LOCUS       DAOPVS010000001        79854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_259_length_79854_cov_57.343112, whole genome shotgun sequence.
ACCESSION   DAOPVS010000001 DAOPVS010000000
VERSION     DAOPVS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 79854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 79854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79854
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_79854_cov_57.343112"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..924
                     /locus_tag="PKL11_00005"
     CDS             76..924
                     /locus_tag="PKL11_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ12006.1"
                     /translation="MIKIVADTTSCIAPEKAAELGIAYIPQIIIFGDDSYRDDNEMDS
                     ATFLKRLRASSSLPKTAAPPPVLYQPIYQEFAQPGNTIFVICPSAEVSGTVRSATVAA
                     QEFPKADIRIIDTRSIGSGLGSVVLQAVEWVKEGVDAATLESNLRDMISREHVYFMVD
                     TLEYLYKGGRIGGAQMLFGSLLQVKPILTVKDGRTEPVESQRTHKRALERVKQMVYSD
                     CPHGPQAHITIMHGDAIEEASKLADELKANLGLTEVPTYQLPPAIMVHAGPGTLAISY
                     FVEKAS"
     gene            943..1608
                     /locus_tag="PKL11_00010"
     CDS             943..1608
                     /locus_tag="PKL11_00010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ12007.1"
                     /translation="MSFLFEDSTLLAINKPAGLRSIADGYDASLPSVRALLSKRYGPV
                     WIIHRLDKDTSGVLLLARNAEAHRQMSIQFENRQVHKTYHAVVRGIPPWREITSKESL
                     RVNGDRKHRTIIDPALGRAAKTDFAVLEQFGDCALIAARPRTGYTHQIRAHLSALGYP
                     ILSDDLYGIRGAPLPGPDERISRLALHAAGIEFSHPQTGRFISIEAPYPADFAHLLMT
                     LKQ"
     gene            complement(1670..3949)
                     /locus_tag="PKL11_00015"
     CDS             complement(1670..3949)
                     /locus_tag="PKL11_00015"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNZ12008.1"
                     /translation="MDIFGLAERGLKTPFEQIAILCVLLVAFISLLYAWLLRGNVLKK
                     DKGTPKMQEVWDAIRVGANSYLSRQLKTILPAILLLGVALFLSVYVVEPTKEAVDRFG
                     DNAVIAVSVGRTIAFFMGAFFSLTVGQLGMRMAIQASVRSASAARRDFNESLSIAYYA
                     GTITGMLTDGLGLFGGTVIFIIFGTAAPDALLGFGFGGTLLALFMRVGGGIYTKAADV
                     GADLVGKVEKDIPEDDPRNAAVVADLVGDNVGDCAGMAADIFESYEVTIVSALILGLV
                     LYESTGSIVWIVYPLIIRAIGVLSSILGTFTVPIWEKFPIKFLRAHDAEEAMFRSYEV
                     SSFNTILWAFILAITYVGDWRMGMLTTIGVGLAVAFNPLTSYFTATKKAPVQDIVKST
                     DTGPATTILSGLSVGMESSVWALVVIVISFVLSLVLYEADGANYVLYAVAMIGIGMLS
                     HTGNNVAMDSYGPISDNANGIAEMAWHGMTDPETVKARQIMADLDAVGNTTKAITKGV
                     AIASAVIAAVSLFASFMADINKVAPGTLTAIDISSTKVFIGFLLGGALPWLFSSLSIR
                     AVTRAAAQVVQEVRAQFRDKAVWEGTKKPDYARVVGISTRSAQKELIPLAIISVVLPL
                     VVGLVLQVEALGGFLAGVILSGQLLAVFMANAGGAWDNAKKAIEDEPRDLAANTGKGS
                     ERHKAGVVGDTVGDPLKDTAGPALNPMIKVVNLVSLLAAPIIVQHQTLGWGGWALALV
                     LTAAIVWAILQSKKSSPAL"
     gene            4390..5601
                     /locus_tag="PKL11_00020"
     CDS             4390..5601
                     /locus_tag="PKL11_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ12009.1"
                     /translation="MENPFINVVDEEDEEYPAIARLIELGRQKTFVTIDDILHFFPDA
                     EQDVEQLEEAFAALMSAGIPYLEDEAPLAEPSEDELILEEEPNERAPEVAIDDLENID
                     TDDTIGLYLKEVSRVPLLTAEEEVELAQRIERGRMAREELARGNVNTRRRLELRKLIE
                     DGWAAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRATKKFDYRRGHKFS
                     TYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLTQRLGREPTVDELAHS
                     LDVPPKKVENMIQVARRPLSLETPTDDEEDSVLGDFIEDDEAPPPDDTVTYNLLREHL
                     EEVLNSLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQALSRLRHPT
                     IRRKLRDYLGE"
     gene            5737..9573
                     /locus_tag="PKL11_00025"
     CDS             5737..9573
                     /locus_tag="PKL11_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ12010.1"
                     /translation="MAKISLRAYQRDIENLIERGQTDEAIAHCKYILRYYPKHIETYR
                     LLGKAYIESQRYTEASDILQRVLSVIPDDLVAQIGMSIIREDEGNLDASIYHMERAFE
                     IQPSNVAVQEELKRLYGRRDGVEPSKIRLTRGALVRMYARGELHQQAIGEIRAALAEE
                     PNRIDLEVILAKMAVLSGKKVEAVEICGRVLAKLPYCAEANRLLAEILPETSRAEDAK
                     IYQQRLIDLDPYLGYISPTTPTTNDVPENAVALERLDWQPGETQPDTRPQWAQAVGAS
                     WDTREEEAPAADWLSAAMPPAEQPSPAAQPSAPDTGIPDFLQAAGWSVSDGDATSRPE
                     TQVSGLEQGEDLGAGELAAAEMPDWLQSIAPPQTGAPAESGTLETEEDSARWLDQMMA
                     AQPPAESVESIAPIDAAAVPPVDLSAMDMPDWLSGLTGETPAENAPSPEPVVGEELAA
                     PADTVPDWLSGLTGETPTTEVPEAEAELFAIEAEAPAEPAEALPDWLSGLTGEAPAFE
                     APEVEAELPAIEAEIPAEPAEVLPDWLSGLTGEAPAAEVPEVEAEFPAIEAEIPAEPA
                     EALPDWLSNIAGETPIIETPEIKAETEIPVLEPEMPAERAETLPDWLSVPTDELPVEA
                     EPAAPQTEATPEWMPETALPEEELVRSETEGAPTIDQALPDWLAGIGEPVEEQPEPQA
                     AEPVAEAPTEDAGFAWLEALAARQGAEANELLVAAEDRRETPPEWATEAENAETAQAP
                     IEELAEPVAEAATELIETPVEEIVETTAVISELPTEAAPTAEESTTAASEDEAFAWLE
                     ALAARQGAEADELLVAAEDRRETPPDWALEAEKPQTEAVEIPEPEAVAAEPVIETAAD
                     LVVEAVEPEAKIGTEPAAELPAEAAAPQTTSTSAEDEAFAWLEALAARQGAEAEELLV
                     AEADRRETPPDWALQAEEPVGTTASSEEPESATVEDTRPTRIRPEAEVSPTEIVPEVT
                     MPVTEAHAEVIEEQPVVGEVPAEPVSTAAATPSEDEAFAWLEALAARQGAEAEELLVA
                     EADRSETPPEWVQEAEASAPEALVETPSEEIIEPVQQLEEAPEPLGGIEPSEEMVAKA
                     PEQPVAEMPAWIAAAVAEEASQPEPPQLEPEAAELEPATTEEAIPDWLQGLAPTEEKP
                     QESVLEWLGREEGGQPITPPRFTEPQVVDQRESPVEEEPVSLPAEEVKDAAEVHRQAQ
                     EALWKGDLKTAMALYNRLIENGLMLEEIIHDLRDALYRYPVDVEVWQTLGDAYARSNL
                     LQEALETYTKAEELIR"
     gene            9590..10045
                     /gene="ndk"
                     /locus_tag="PKL11_00030"
     CDS             9590..10045
                     /gene="ndk"
                     /locus_tag="PKL11_00030"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNZ12011.1"
                     /translation="MERTLVLIKPDAVQRGLMGDIIVRLERRGLKCVAAKFLQVSKEL
                     AEQHYAVHKGKPFYEGLVRYITASPVMAMVWEGPNAIAAVRQTMGSTRPTEAAPGTVR
                     HDFALEMSRNLTHASDAPETAAQEIALWFKPDEIAPWDRCTDPWLFGKN"
     gene            10222..11832
                     /locus_tag="PKL11_00035"
     CDS             10222..11832
                     /locus_tag="PKL11_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12012.1"
                     /translation="MESPDTAAFTYSMLRLPSCMPQIRTVILGQSAELFARSGYPNIE
                     NWQPVSARARRRRCYFNRQDILACFIASRSDIDDVVPALTAYQIEWNKLHDLLKECPE
                     GTIEDAAKGNSVAFTNLAKVLRVTPNDLQRLWVVWGALETAQNGETNDARAARFLQRI
                     AQQKCALRLRLLGGSLSDYWRATRAWWDNIEHQQPGLLDRPVYFISSNTHSLSNLLSG
                     FGLTLQSALEDYLRLPENLKLQEEWQTIQSGQLGSSRENFLYYILKKYQQSEYGREAG
                     QQQIDCEKNAGIYRVPSEHYFDIDAQVVEINKLDITNLDPRLKEKGPAYPGDLSILRN
                     SNALILNVDYPLGLAAYNILSKVAEHASPVLGIYIMGKAASLNGVVGDVMIPNVVHDE
                     HSHNTYLYQNAFTAASVSPYLVYGTVLDNQKAVSVLGTFLQNAEIMDVFYREGYTDIE
                     MEAGPYLSAIYEMSRPKRHPVNELVNLYGVPFDIGVLHYVSDTPLSKGQNLGAGTMSY
                     YGMDSTYATSIAILRRIFQIEALRLSAD"
     gene            complement(11812..12642)
                     /locus_tag="PKL11_00040"
     CDS             complement(11812..12642)
                     /locus_tag="PKL11_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ12013.1"
                     /translation="MTVPLIIVDALLTIVIFYGFYIEPFWLTVSRVQIETPDLKPGET
                     LRIVHLSDLHTEHLTRREPEIVARVAALSPDVIFWTGDYLNLSFLEHDGVMTDTRQLL
                     GQLSAPYGVYAVQGSVDGPLQMQALFSDSSIRVLNDEWIDLPWPGSPVRILGIANRDY
                     PLDRQEFMQLAPDISESAYSILLYHSPDLTPEAAEIGMDLVLAGHTHGGQIRLPFYGA
                     VVTFSRYGKRFEMGQYQIQDTTLYVSRGLGMEGWWFAPRARFLCPPEIVVIDLIGAQP
                     "
     gene            13000..14814
                     /locus_tag="PKL11_00045"
     CDS             13000..14814
                     /locus_tag="PKL11_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12014.1"
                     /translation="MSRSPRIPDYDLQKVVTPNRLLGLWRMMTGFRLKYLGATLSLGV
                     SALSKTLTYMLLRYFVDHYLVAQTGSVPLIFIALGFLGLATIEGSMTFTSGRLAAQTA
                     EGITRRLRNYLFDHIQQLSFSYHDQAQTGDLIERCTSDVDAMRRFFSDQAIGIGRIIL
                     LFTINFIALININPTLAVVSIVVLPLIVAASFFFFKRVSKAYAAYQEQEATLSTTLQE
                     NLTGVRVVKAFARQKYEMDKFERDNWEKFLRGKKLNTMHSLFWPVSDVLCGFQMLLGY
                     FTGAVMAINGTISVGDYLAYTGLITYIIFPMRNLGRIIVQLSTGMVSFGRVREIIKER
                     REPLDEGDYLPEKAVRGELQFLNIGFEYESGSSVLSEINFEVLPGQAIALLGATGSGK
                     TTLVNLLPRFYEYTHGKIELDGVPLQRYPRRYLRQQIGIVEQEPFLFSRSIRENISYG
                     VGRDVDMVEIEAAARAAAIHDVILSFPQRYDTIVGEKGVTLSGGQKQRVAIARTLLKN
                     PRILILDDSTSSVDTETEAEIRAAMETLMKNRTTFIIAHRIQSIMNADLILVMDQGRI
                     IQSGQHETLVSQPGMYRDIYDIQTRIEAELEKEIASVS"
     gene            14804..16633
                     /locus_tag="PKL11_00050"
     CDS             14804..16633
                     /locus_tag="PKL11_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12015.1"
                     /translation="MSANYIEEEEFQTRFSGKIFVRILGLIKPHWPQVVGFLLAILVV
                     SGLDAWFTYLSKQIVDNGIVPRNVDSLLKTLELYGSLIVIQAIATFFFIFFVSILGER
                     VRYDLRRMMFNHLQKLSLSYYSRTPVGWIIARVTSDTERVSDMITWGLLDSAWAVMNI
                     TTSLVFMFAINWRLTLIVLVILPILLYVAIQFRKRILVQYRKVRKQNSKIVGAYNENI
                     TGVRVIKALGREQGNLEEFGVVTGEMYKASYRAAWLSALFLPSVQIITAFAVGSIVYY
                     GGLQANIGGVTVGGIQAFISYVTFMMWPIQDLARVFAEMQHAIASAERIFSLTDAVPD
                     VADRPGSVDPGTIQGDIEFDHVTFAYDDEGKQVLRDFSLLVKRGETIALVGPTGGGKS
                     TIVNLLCRFYEPQAGVIRIGGRDYTEISMHAIQSRIGVVLQTPHLFSGTIRENIRYGR
                     LDATDAEVEEAARIVGAHDFIQRFEHHYDEPVGEGGNLLSTGQKQLISLARAVLAQPE
                     IFIMDEATSSVDTLTEALIQRGMETLMKGRTSFVIAHRLSTIKRADRILVIEDGRIAE
                     MGTHGELLRKGGKYYQLYTKQFRHEMNEAFDPLRQPGKKEQPA"
     gene            complement(16682..17320)
                     /locus_tag="PKL11_00055"
     CDS             complement(16682..17320)
                     /locus_tag="PKL11_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNZ12016.1"
                     /translation="MSHGKIDHSDVPIRLFKSDFMEFFTHVSPITITVIWLPVAVYAL
                     VRSILDVPVVGVWWHIPVGFIGGLFLWTFSEYILHRFLFHYPAKTPATQRIFFLFHGV
                     HHAQPQLKTRLVMPPVVSIPLALIFYGLFYLVFAVLLSAPQWIAPVFSAFITGYLAYD
                     ITHYATHHFPMKTRYLKFLRKYHMLHHFKTPEQRYGVTSPIWDRVFGTYPAE"
     gene            17404..17628
                     /locus_tag="PKL11_00060"
     CDS             17404..17628
                     /locus_tag="PKL11_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12017.1"
                     /translation="MEENKGKVPWYLWPFWAIWKLVIGIIALTGRLVGAILGLVFLIV
                     GVVLTVTVIGAIVGVPFIIFGLLLVIRSLF"
     gene            complement(17609..18163)
                     /gene="yjjX"
                     /locus_tag="PKL11_00065"
     CDS             complement(17609..18163)
                     /gene="yjjX"
                     /locus_tag="PKL11_00065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HNZ12018.1"
                     /translation="MPRVVVASKNPVKIESAQLGFARMFPSRSYDFIGVSVPSGVSAQ
                     PFSDLETYQGARQRALNARQAHPDADYWVGIEGGVDTLMDDLIGFAWVVICSAQQMGW
                     ARTGTFCLPPQVSKLVRQGKELGEADDIVFSRSNSKQENGAVGILTDNAINRAQFYEP
                     AVMLALIPFKNPGLYPADIRTDCE"
     gene            complement(18148..18621)
                     /locus_tag="PKL11_00070"
     CDS             complement(18148..18621)
                     /locus_tag="PKL11_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HNZ12019.1"
                     /translation="MTLPDSPQPFWKTKSMSEMSPEEWESLCDGCAQCCLIRLEDEDT
                     GEIATTYVVCRYLDLHACRCRVYKRRTRLVPTCLQITPELIPQLSWMPKTCAYRLIYE
                     DKDLPSWHPLVSGNRQSVHRAGMSVRGRVVPESVVNEENLEDYITDEWDDPCPEW"
     gene            complement(18618..19622)
                     /locus_tag="PKL11_00075"
     CDS             complement(18618..19622)
                     /locus_tag="PKL11_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12020.1"
                     /translation="MELLILGGTRFLGRALIELALASGHQITLFHRGKSNPDLFPEAL
                     HLIGDRETDLSLLAGRHWDAVIDTSAYIPRVARLAAQALTSLTDQYTFISTLSVYADS
                     SQIGIDETASVGKLDDETVEQVDGQTYGPLKALCEQEILRVFPNNSLIIRPGLIVGRY
                     DLSDRFTYWPHRLFSGGDVLVPGTPQRKIQFIDVYDLSAWILRLIENGASGIFNADSP
                     AGMHTMGELMDACCQLAQSLVQLTWAADDFLLSEGVDPWMGLPLWMPENDPTQAGFYA
                     FDISKARQSGLTYRPLSETVRETLDWELSRPPHTWRAGISSQREAELLEKWHCNQDRK
                     "
     gene            19703..20527
                     /locus_tag="PKL11_00080"
     CDS             19703..20527
                     /locus_tag="PKL11_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HNZ12021.1"
                     /translation="MKKHLCLFGVALLGILINSCTPITITPLPTTSAAAPGNHADIWR
                     PAMNDTWQIQFSGDLDTTIEADIYVVDGFEITPEEIESLHQYGRKVICYLNAGAWEDW
                     RPDAPDYPTKVLGEAMEGWPGERWLDIRQLNLLMPLIETRMDICQSKGCDGIDPDNIH
                     GYTQFTGFELSYNDQIRFNQFLADAAHQRGMSIGLKNDLEQIDDLVEDFDWALNEQCF
                     EYDECDYLASFIKANKPVFNIEYNLEAEGFCSRAEEAGFYSQIKNLELDAWRITCR"
     gene            20518..20823
                     /locus_tag="PKL11_00085"
     CDS             20518..20823
                     /locus_tag="PKL11_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNZ12022.1"
                     /translation="MSVKHFMVEITYTVPAEALSEILPKHRAFLQTGYDQGVLLMSGP
                     QEPRTGGIVIARAESVSALKDFFKNDPYQINQVATYRFVEFVPVKHQPWLGQWVNQE"
     gene            complement(20831..21871)
                     /locus_tag="PKL11_00090"
     CDS             complement(20831..21871)
                     /locus_tag="PKL11_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNZ12023.1"
                     /translation="MKTQRKPVYRKIQAGWRDTLLLLKEFWFPLFVFFLLVVGGGEAY
                     YLLAQRTGESIGGLGASIYHVLSLIFFQPMAGYPQAWYLQLFYYILPFLGLGILALGV
                     TDFASLLFNRRARGKEWEMAVASTFSKHVVLVGLGHLGYRVVKQLRELDEDVVVVELS
                     PNPDLLASVHRLDVPVIEDDASREVILEAAGIRRAKAILLCTQNDSLNLQIALKARNL
                     NPQIQVVVRIFDDDFAEALRQQFGFRALSATGMAAPVFAATAVEIDITPPIVIAGQPN
                     SLARIEISAQSKLIGLQLCELEQRFDLSVVMQSRSGESDVHPAGTSTVQAGDMLAVLG
                     KPEQINRLAHQN"
     gene            complement(21868..22590)
                     /locus_tag="PKL11_00095"
     CDS             complement(21868..22590)
                     /locus_tag="PKL11_00095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNZ12024.1"
                     /translation="MEIQVAVAKINKHGSEESGDTLELVERPGGGISVVMADGKASGH
                     EAKSVSAMVVRRVIGLLADGVRDGAAARAASDTLYTERSGRAGVYLNILSADLQTGTL
                     VLSRNNPTPVFIAQNERVECISTESGAIGVARSVRPSISEIPLQVWMTVVMYTDGVSH
                     AGEASGESLDICTHLLAMIEEQSPTAQQIADSLLQQAVRLDHGRPNDDMTVVALCVFP
                     STGDPIRRLSARFPVPEYPQDQ"
     gene            complement(22644..23294)
                     /locus_tag="PKL11_00100"
     CDS             complement(22644..23294)
                     /locus_tag="PKL11_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNZ12025.1"
                     /translation="MASQVLYRFLTWFFRLLYHQLAWSYDIVAAVVSLGRWNSWIRAI
                     VPYINGECILELGPGTGVLLSALAGDLRFCVGVDESAQMLRQARRRVRRTNSRNVHLI
                     RARGGSIPIQSYSCNAVVATFPTEYIYESRTVHEIRRVLQPGGQVVILISAQVGGGRL
                     SEKLLRKIYAITRQSPGSDTDLEVFRRPYAEAGFTTRLEWITSGRDRLLILIAKSS"
     gene            23432..23502
                     /locus_tag="PKL11_00105"
     tRNA            23432..23502
                     /locus_tag="PKL11_00105"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23464..23466,aa:Gln,seq:ctg)
     gene            23666..24304
                     /locus_tag="PKL11_00110"
     CDS             23666..24304
                     /locus_tag="PKL11_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNZ12026.1"
                     /translation="MEKVVLHATRRSVTGKQVGVLRREGKLPAVLYGHGFETTAILLD
                     LREATRTLAHLTGSSLITIELDGKGHAALVREKQRDYIRNVLLHVDFQVVSMTEKIRA
                     QVPVVITGVAPAVKDFNGVVVTGLNEIEVEALPQDLPQQFVIDIANLVNIGDGIYVRD
                     VVVSDKVHIHEGKDEMIVVITAEMGEEAAEEAAEAPAEPEVIERGKKEEAEE"
     gene            complement(24366..24881)
                     /locus_tag="PKL11_00115"
     CDS             complement(24366..24881)
                     /locus_tag="PKL11_00115"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNZ12027.1"
                     /translation="MPVPEGFRRAAVLLPLLRMDHSWHLLLTHRSHMVGEHKGQVAFP
                     GGGADPEDRDSICTALREANEEIGLLPEDVNILGCLQPLKTITNYFVTPVVGVIPWPY
                     DLDLSPFEVARAFTIPLFWLADPKNWTEKPFIAKGKTDEHVIYYDLYEGELLWGISAR
                     ITLNFLTALGF"
     gene            complement(24959..25525)
                     /locus_tag="PKL11_00120"
     CDS             complement(24959..25525)
                     /locus_tag="PKL11_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12028.1"
                     /translation="MPPRKINRPGLPPPRRGPAHAGPQTLLEQARRWMNNGDYHRASV
                     AFEKLAREADRLAPVRSPLMYFQAGRCRVLMGDPESGLGLIYRALNALALQARWPELH
                     RSAQITISELRDLGAPAQAEALQTWIEQKSASAPDTLPGDVSPVRENIQLPLNCPNCG
                     APLLAKDLEWIDASTVVCNFCGGLVRPQ"
     gene            complement(25506..26396)
                     /locus_tag="PKL11_00125"
     CDS             complement(25506..26396)
                     /locus_tag="PKL11_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNZ12029.1"
                     /translation="MTWNLHGTHANDGDLAELVGLRHKRFILKLEAGAAFHTHRGIVY
                     HDTLIGKPWGSQVFSHNGAPFFLLQPSLADLLRELPRSSQILYPKEIGFILVTMGIGE
                     GQHILEAGTGSGALTSAFAFNVGKAGHITSYDVREDMQNTAARNLDRLGLRERVTLKQ
                     RNIEEGFDETGVDALFLDVPNPYDYIAQARSALKAGGFFGSILPTVNQVEKLLVALRQ
                     NDFAFIEVCEILLRYYKAEPTRLRPTDRMVAHTGYLIFARPVIIDRSVDSGTLLAEAG
                     LVSVPAPDSGAEVDDATPED"
     gene            complement(26393..28294)
                     /locus_tag="PKL11_00130"
     CDS             complement(26393..28294)
                     /locus_tag="PKL11_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNZ12030.1"
                     /translation="MFHVESLLSARLFVSPQLVGDQIYFISNLSGRLSLYRMKFGASV
                     PEPLLPPDIALQNPHLVGGAPFLVFPALGKILVLLDHDGDENYLPMLIPLDGGFPEPA
                     FNGFFEGTRVHVTLGDDKHNLVYLGAESRQEPVVRSYQCNLATGSITELAVSPWGTYP
                     AAFSKDHRRIALLEGYTFGDVTVYLWENGTRKVLYGVPLEDRLPSQEIPLNGIGSLNF
                     TPDEQGLLVTTALYTDTFSPAYLDLSHPGELKPIEVIGLAHSGVGEHVSLEHLKDNRY
                     LLEYNIDGCSWLYEVLFDASKLSLTVSHVLVGQGELANGVLFSHLYDEAGDRMALSFS
                     TATSPTQIYTIDGDQVVRHTNERVLGIPTELLSPGEDASFVSYDGTRVSARLYLPAPA
                     LGFDGKRPLVYYIHGGPQSQERPDFAWFSIPLIQYLTLNGFAVFVPNARGSTGYGLSY
                     TKQVDHDWGGRDRLDHVHALEVLAQHPRVDVHRAGVVGRSYGGYMTLTQAMRHPDLWR
                     GAVDMFGPYDLITFMERIPETWKPYFRIAVGDPIEDREFLMERSPKTYAHQLACPMLV
                     IQGKNDPRVVERESRDVVEYLRSQGKQVEYLMFENEGHDVLKFENRVRCYLAITEFFS
                     QHLGLEPRQ"
     gene            complement(28298..28582)
                     /locus_tag="PKL11_00135"
     CDS             complement(28298..28582)
                     /locus_tag="PKL11_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12031.1"
                     /translation="MTIQSVIFGTLIAALLGFVFHLWKGGGLGRMILYLLLSLIGFWA
                     GHLLGAALNWTFASIGPLHLGTAILGSLAVLGTGYWLSLVQVEKKTERRR"
     gene            complement(28579..29673)
                     /gene="mnmA"
                     /locus_tag="PKL11_00140"
     CDS             complement(28579..29673)
                     /gene="mnmA"
                     /locus_tag="PKL11_00140"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNZ12032.1"
                     /translation="MTSDRPRVAVGLSGGVDSSVAAALLLEQGYSVIGIMLRLWSIPG
                     QEEDNRCCTPDAMAVARRVAAKLGIPFYALDAGPLFYQHVVQNFIQEYAAGQTPNPCL
                     LCNRTIRWGYMLEQAQNLGADYLATGHYARTHKDSNGQIHLLRGLDVHKDQSYVLSLL
                     NQSQLQHTIFPLGHLTKPEVRQRAAQLGLAAADRPDSQDLCFLGDADYRDFLRSSAPE
                     SVQPGPILNRQGQQLGLHQGLPFYTLGQRKGLRIAAPEPLYVISKNIERNELIVGRAD
                     ELGRRNLVAGPINWILGEPSAAQFDAVVKIRYKSSLMRAHITIHDNHAHMAFQTPLRD
                     ITPGQVAVMYNGDEVLGGGIISAWDEEDAA"
     gene            complement(29670..30839)
                     /locus_tag="PKL11_00145"
     CDS             complement(29670..30839)
                     /locus_tag="PKL11_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNZ12033.1"
                     /translation="MTQPNPIYLDYAATTPVDTRVQRAIMDAWAANYGNPSSVHWFGQ
                     RAEAALESAREAVAAFLNAHPSEIYFTSCGTESDNFALRGVARARRMQTGANHILVSP
                     VEHSAVLHTAQQLAALENFDLELLPVDAFGRVDPASVASRIRLSTALVSVVYANNEIG
                     SINPISEIGRVCRQAGIPFHTDAVQAAAYLPMDVQADNLDLLSLGAHKLYGPKSGGVL
                     FVRRGVPIQAVQTGGKQENGLRAGTQDIPSALGIAEALRLIQLERDDHTAHFLQLRDR
                     LIAGVLASIPDVRLTGHPRSRLPNHASFVFEDVDGNTLLALLDAAGFACSSGSACKSG
                     SPRPSEILLALEIPPEWALGSLRVTVGRYTTLEDIENFLTILPQKVTQARRGEHK"
     gene            30966..32387
                     /locus_tag="PKL11_00150"
     CDS             30966..32387
                     /locus_tag="PKL11_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNZ12034.1"
                     /translation="MSTPIRFGTDGWRGVIAEDYTFDNVRRCTQGFADYMLRHGKKDQ
                     WFIVGHDKRFHSENFALAVAEVLAGNGLRVYLTNGATPTPVIAFSVVNRKAAGAINIT
                     ASHNPPTDNGFKVRDENGGAISPEGLNEIESLIPDSVAGVRRMKAAEAEAAGRLVRFD
                     PAPAYIEHLADLIDLQPIRDAGLNIVVDAMWGNGAGWFSHLLAGGTTKVTEIHNTRNP
                     IFPEMKRPEPIQPNINVGLQTTVDLGADVLLITDGDADRVGVGDEQGRFINQLQVYAL
                     LALYMLEVRGERGPIVKTLSTTSMLEKLGKIYDIPIYETGVGFKYVAPMMIETQAMMG
                     GEESGGFAFRDNVPERDGILAGLFILDMMVKLKLKPSELIDLLYSKVGAHYYDRIDTS
                     FSGDRKAREDMILSAKPETIGGLKVTGLNTMDGYKFSLEDGGWVLIRFSGTEPIMRVY
                     CETTHKEAVQAILKDGMKIAGLA"
     gene            32454..33197
                     /locus_tag="PKL11_00155"
     CDS             32454..33197
                     /locus_tag="PKL11_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ12035.1"
                     /translation="MERAQSAGVDRIMVPGIDLESSRAAIRLAEQHDAIFAAVGVHPN
                     DAAGWDKAWFDELEQMAKHPRVVAVGEIGLDYYRDRCPRAKQLAAFEAQLALAKACDL
                     PVVLHSRQSLGDMWAVLQSWEVDRRQQQAPFEAAYGVLHSYEGPAETACALIETGFYI
                     GINGPVTFKNALDRHDVVRVLPLERLLLETDAPYLTPHPHRGQRNEPAYIPLIAQKIA
                     DLKAVPVATVAETTSINADRLFRWRAFVA"
     gene            33157..34161
                     /locus_tag="PKL11_00160"
     CDS             33157..34161
                     /locus_tag="PKL11_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ12036.1"
                     /translation="MQTGCLDGELLLRKSAQFLSLVQEDLAEVEALLRTQAHGYHPDL
                     QAAFNHLLQSGGKRVRPAVTMLIGHMLGAPHDQCITLAASIEMLHTATLVHDDLIDGS
                     LLRRGSPTLNAQWSPGATVLTGDFLFSIAARLAAETDSIEVMKVFARTLTIIVNGEIS
                     QLFTSRCQASRENYYQRIYAKTGSLFETSAYAAALISPVDGKISEAMKQYGYEIGIAF
                     QLVDDVLDFTADEATLGKPVGSDLRQGLVTLPAILYAESNPQDPAAADILNGRCVQEE
                     AEINRLVEAICKSDAITRAMAEASNYVSRAMECLQTAPNSASKAALEDLAQYIVARNL
                     "
     gene            complement(34198..34279)
                     /locus_tag="PKL11_00165"
     tRNA            complement(34198..34279)
                     /locus_tag="PKL11_00165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34243..34245),aa:Leu,seq:gag)
     gene            34339..35379
                     /gene="plsX"
                     /locus_tag="PKL11_00170"
     CDS             34339..35379
                     /gene="plsX"
                     /locus_tag="PKL11_00170"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNZ12037.1"
                     /translation="MAIVLDAMGSDHYPQPELQAAVEAARLYGEEILLVGKEAMLADG
                     LRQLNTGGYPVRIVDAPDVLEMGEKPVDAARRKPRNSMAVGLELVKKGEASAFVTAGN
                     TGGAMFNAYRTLRFLDGVLRPALSALFPVRGGRAVITDIGANSDCRPEFLLQFAVLGS
                     VYARRILKIENPRIGLLSNGEEAGKGNQLVRDTYPLLEQSGLNFVGNLEGKEVYGGMA
                     DVVITDGFTGNVFLKSSEAVAKFLLEMMKEQLTASLPAKLAGAMAKPYLMNLKKVMDP
                     AEVGAAPLLGVNGLVFVGHGRSDARALVSAIRAAREAVQADLLGAMRTAIQEQLAQID
                     ALITQNASGQKE"
     gene            35384..36073
                     /locus_tag="PKL11_00175"
     CDS             35384..36073
                     /locus_tag="PKL11_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12038.1"
                     /translation="MRIVMDEKMVKRNQKVAQYTSLASLVILVIGLIVSFTNPEQIII
                     SFGALLLGFLLSQVGIFYGNRWGKSPRADEILNDELKGLDERYIIYHYCTPVPHLLVG
                     PAGVWVLAPYSQAGKIEYDAAKDRWSHRGGNWHLKVFGQEGLGRPDRDAKITLQDVNK
                     YLHDLAGDDPRRPEAQLALIFTNAKAEVQSQETPLVAMPVAKLKDFIRRKAKEVNFPA
                     DTQTWLEEELP"
     gene            complement(36133..36894)
                     /locus_tag="PKL11_00180"
     CDS             complement(36133..36894)
                     /locus_tag="PKL11_00180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ12039.1"
                     /translation="MEERLQKILARFGYGSRRASETLIIAGRVSVNGKIATLGAKADL
                     QNDEIIVDGRKLPKTEVQMVYVMLHKPRGVLSDSGAEDERKTVRDLIPVPGHLFTVGR
                     LDLDSEGLILLTNDGELANRLTHPRYGHEKEYRVLVEERPDDRQLAALRHGVVLEDGH
                     RTAPADVRIENTAGKNIWLRITLREGRKRQIREMGIRIGLPVLRIVRIRIGTLLLGDL
                     KPGEWRYLTAYEIRRLSATANPEPTHKKYPPRKVA"
     gene            complement(36900..37472)
                     /gene="scpB"
                     /locus_tag="PKL11_00185"
     CDS             complement(36900..37472)
                     /gene="scpB"
                     /locus_tag="PKL11_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNZ12040.1"
                     /translation="MTDASPESTLPLSLEACLEAILFVAASPVPVAQLAQALGRPLEA
                     VEQAIANLEVQLAQGRGISLQRHEGKIQITTAPMLASIVENFLGLEATARLSRAALET
                     LAIIAYRQPVTRPGIDAIRGVNSDGVLKSLLSKGLVQEMGRAEGPGRPILYGTTTEFL
                     QHFGLSSLGQMPPYELPETPDEERNVILKD"
     gene            37489..39129
                     /locus_tag="PKL11_00190"
     CDS             37489..39129
                     /locus_tag="PKL11_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNZ12041.1"
                     /translation="MKVKFLIEFNYTCCMMNCHPQPKNAKITGWLLLLLLLAACTPLA
                     LTNSGMVAVSIQVDGEVIEANTPAGTTVADTLENNDIILDTLDRVEPPSYTIIAEPTT
                     ITVVRIREEYEIEQAIVPFEQQTVPNESLPDRRTMLIQLGENGLQEITYRRVFENGIE
                     TSRTVFKVVTLNEARPEIVMIGVQSPFTPISIPGVIAYLTAGNAWVMSSTTGERRPIV
                     TTGDLDGRVFALSPTGEWLLYTRKSEGEEKSINTLWVVRITDDEPEPFSLDAENIIHF
                     AAWIPSDMLMVLYSTVEPRATAPGWQANNDLRRIRFSENAYIRDEEIMEANSGGIYGW
                     WGMNFAISPDGTQVAYSRPDSVGVVDLESGATSTLLDLLPLQTGGDWAWAPGISWAPD
                     QQILYSSAHIPMSGLANPEASPLFNLSAIVLENGAAFPVVSQTGMFAYPTASPDTLPG
                     DRYLVAYLKAILPEQSDTSRYQLMLMDRDGSNQKLIYPPEGMSGLEPQQVVWAPATGD
                     EPLWLAFIYQGNLYLYEHGTDIIRQVTGDGLIDRIDWK"
     gene            39153..40112
                     /locus_tag="PKL11_00195"
     CDS             39153..40112
                     /locus_tag="PKL11_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12042.1"
                     /translation="MIVLITGAAGFLGSHLCDYLIEQGHTVIGMDNFITGSPDNLAHL
                     AGHPRFSFIRHDVANFIFVPQKVDAVLHFASPASPNPASPYGYTNLPIQTMKAGALGT
                     HNTLGVAKAHGARYLLASTSEIYGDPLEHPQKETYWGHADPIGVRSVYDEAKRFAEAL
                     TMAYHRYHGLDTRIVRIFNTYGPRMRLDDGRVVPNFIQQALQKEALTVYGDGEQTRSF
                     CYVSDLVAGIYKLLLSSEDMPVNIGNPAEISILEFARTINRLTNNPTSIKFIPEQRLG
                     DDPQRRRPDITRAQQILGWQPTISLEEGITRTIPYFVQKMGLA"
     gene            40109..40561
                     /locus_tag="PKL11_00200"
     CDS             40109..40561
                     /locus_tag="PKL11_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNZ12043.1"
                     /translation="MILTNERERTRFLRFAVVGTIGAVIDFGIFNLLIWLGMRAIVAS
                     AISFIAAVISNFLWNRYWTYPDSRSKPLARQMVQFLLVSLVGLGIRAGLFAILEIPLI
                     SLFEKLALPLPLSAAFLGHNLTLAIAILIVLLWNFLANRYWTYNDVSK"
     gene            40653..41768
                     /locus_tag="PKL11_00205"
     CDS             40653..41768
                     /locus_tag="PKL11_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12044.1"
                     /translation="MDLVSGRNFNTNAPQEDLGLAEPLPYPFLALLGQEDMKLALLLA
                     IINPAMGGVALIGPRGTGKTTAVRSLLDLLPDAERSACFYGCLPEDIEAGGIDAVCPN
                     CAQKYARGEKLTRTERVRLVELPLNARLEDVIGGVDERAALNDRMRLKRGILSQADRN
                     ILFIDEINLLSDEIINAILDAAALGTYTVRRGPITATYRSRFILVGSMNPEEGALRPQ
                     IMDRFGLRVIVTGLNKPEERLEAYLRAQAHRRNPRASIAQFMAATLQARMEIEAARSL
                     LPKVTIPEQIARAGITLIQKLQIASLRAEITLFEAAKAYAAADNRETVTLSDLKVVAP
                     LALRLRRSDYMQQYQAKQREEDSEMIRIMEDTLKGEG"
     gene            41774..42772
                     /locus_tag="PKL11_00210"
     CDS             41774..42772
                     /locus_tag="PKL11_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ12045.1"
                     /translation="MAKSGTQTHRQRLETLLGGGRPDRTPVALWRHFPVDDQAPERLA
                     AAIIAFQKSYDFDLIKVTPASSFCVKDWGAADIWQGSAEGTRAYGPPVIQKPEDWENL
                     RRLSPHEGALKAQLECLKLVMGAFKPHTPVLQTIFSPLAQAKNLAGKEQLIVHLRQCP
                     EAVHAGLRIITQTTIDFISEALKTGMDGIFYAVQHAQYGLLTEAEFMTFGRAYDLPVL
                     ATAQSLWLNMGHIHGEQIMFQQITDYPVQILNWHDQHTWPSLKEALSLYPGLVCGGLR
                     QWETLAYGTPEQIWAEARAAQAQTQGKRFILGTGCVTPIIAPHGNLMAVRNSVEAA"
     gene            42777..43373
                     /gene="rsmD"
                     /locus_tag="PKL11_00215"
     CDS             42777..43373
                     /gene="rsmD"
                     /locus_tag="PKL11_00215"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNZ12046.1"
                     /translation="MSTPRVIGGTARGIRLKDVPGDITRPITDRVKEALFNILGGDII
                     DASLLDLFGGTGAVGIEALSRGAAFVRINDLNRAAITTIRANLEATRLQSHAQVTQGD
                     AFSLLQQAADRAFDYIYIAPPQYKGMWQRAIKLVDDHPNWLSDEAWVIVQIDPVEYEA
                     MSLKNLNEFDQRRYGSTLLVFYERGNDEDDASNAELDL"
     gene            complement(43350..43856)
                     /locus_tag="PKL11_00220"
     CDS             complement(43350..43856)
                     /locus_tag="PKL11_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ12047.1"
                     /translation="MPKQDQGVTPNRYAIIPRTLIFLTSGSDILLLKGAPTKHLWANR
                     YNGIGGHVEPGEDLFFAARRELNEEAGVHDADLWLCGILMVDVEPDRGIGIFIFRGEV
                     DKTTIELVPGSEGELAWVSQASLSNLPLVEDLPVILPRVLASMRSTQPFLGRSFYTDG
                     KLKIEFGV"
     gene            complement(43969..44919)
                     /locus_tag="PKL11_00225"
     CDS             complement(43969..44919)
                     /locus_tag="PKL11_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HNZ12048.1"
                     /translation="MIVTTKKLFEAAYGRYAIGAYNINNIEQTMGLFRGNVDSQAPFI
                     IQISKGARSYTDKRMLEAMIRTANDIFPEAIFAVHLDHGDEAACYDCIESGFYSSVMI
                     DASHEDFDTNIAITKRVVDAAHARGIVVEAELGQLGGVEEHVAVDEANAKLTDPQTAR
                     EFVERTGCDSLAVAIGTSHGAFKFSGNQALRFDVLEQIQQQLPNFPLVMHGSSSVPQE
                     EVERINAAGGILKGAKGVDDRQFLRAAQLGVTKINIDTDGRLVWTRVHREFFRDHPDA
                     IDLRPIGKIFMSEYANFIADKNHKLGSAGQIPTVKQLLGI"
     gene            complement(44936..46108)
                     /locus_tag="PKL11_00230"
     CDS             complement(44936..46108)
                     /locus_tag="PKL11_00230"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ12049.1"
                     /translation="MTTKTLGILTGGGDVPGLNPCIKAVVMGALELGYRVIGIRRGWG
                     GLLHYDLNEPSTHDYYIRELKREDVRTIDRTGGTFLHTSRTNPQRVDPKAVPDFLKNS
                     TWGKPAEGSSNIDYTSHVLNVLANLGIDVLTPIGGDDTLSFAVRLHKEGFPVVAIPKT
                     MDNDVFGTDYCIGFSTAVTRSVDIITNMRTSVGSHERIGIVELFGRNSGETSLISAYL
                     ADVDRAVIPEVPCDVGRLARYLIDDKRGKPSNYAMVTISEGAILEGGEVIESGEADAY
                     GHRKLGGVGVVLSEEVKRLTGQNIMYQQLGYMMRSGAPDSLDRMVAMSFGNLAIQLIR
                     RNETGKMVGLHGGKYTTVPVEMVLAGRKRVDVPSYYDIETYRPKVRDFMGVPMFLS"
     gene            complement(46143..47243)
                     /locus_tag="PKL11_00235"
     CDS             complement(46143..47243)
                     /locus_tag="PKL11_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ12050.1"
                     /translation="MTVHNFGANTDGASPWEEFQQSVQTDLDEARRNLQEVTLMYEQS
                     QAELAKLTQRNAAITAHLQQVRSQVEATSKADIQNAYNAALEAQQRLLVMRGQIEKLQ
                     NDQETLKRYVASLERAHKFLSEGHQPPRSMRAKGGGSAVLELVINAQEQERQRLSRQM
                     HDGPAQALSNFIVQADIVARFFEVDLNRSKEELNALKTTAMSTFQKVRFFISELRPMM
                     LDDLGVVPTIRRYADTLKEQSGADISLNVKGNERRFEPYIEVMIFRSMQELIGGAVRH
                     NQELPYKLQINAQMVIEDRLVRVSVSDNGKGMRPEDESESGVGIKLIRERVEMVGGYM
                     EIDSNVGQGSRITFQIPVEPNAPEPEQELRAM"
     gene            complement(47401..50439)
                     /locus_tag="PKL11_00240"
     CDS             complement(47401..50439)
                     /locus_tag="PKL11_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HNZ12051.1"
                     /translation="MKTKWIVLNFLVILAMLLAACGQQHPGSKDVLYVNLVWHQHQPL
                     YYKDDAGVYTRPWVRVHATKDYYDMAAILQEYPDVHVTFNLTPVLIRQLDDYADNGAK
                     DLYWQLAEKPASDLTDDEKRFILQRFFDANWDHIIAVYPRYQELLDLRGGTDTAAIES
                     ALATFTEQDYRDLQIWFNLAWFDPDLLAADPLKSLVEKGRNFTEADKQILFAEALRII
                     QAVIPLHKEMQSDGQIEVITTPFAHPILPLLVDTNLAREGNPGVETPPQFSYPQDALT
                     HLERSVAIYEENFGQPPQGLWPGEGAVAQTIVPFVIKSGYTWMATGEPVLAKSLNLDG
                     FTRDAAETVQQADDLYRPYYVTDANGNQLAVFFRDGVISDKVGFTYSGISGEAAAEDL
                     IQRLENIRTELKDEGAEGPHIVSIILDGENAWENYDNDGKEFFHSLYSLLSESKTLQT
                     VTPSQYLEWFPEQRSLDNLFAAAWFSPNYDTWLGEAEENMAWDYLRQTRAVLAKYDIS
                     KVRTASPEAIAQAQDFMYLAEGSDWFWWYGADQDSGQDDYFDTGFRALLKGVFDSLGE
                     PVPNFVNVPIIQPRPVQAAQPVQGMSTPVIDGKIDGDEWSLGAAYPAEVQTPFASGLG
                     YTYDANNLYLRLDLTRPMSASDQVGFYFVAPRAAGSNPFVNISADEPLLLGVAATHLV
                     EVTAKGSLSAYTGGETGWAKMEALGEASVGDSVIEVAIPFKLFGEMETGDDIRLLTVA
                     QPDNQFIPTGGPAQIIVPEIGNATVVFEVEDPEGDDNGPGTYTYPTDAIFVGKMFDLK
                     SFRVAYDENNIIFKFSFYGAIPNPWGSPNNLAVQTLDVYIDKDPGGGTGARLLLPGRN
                     AALEAGSGWDYAVWAEGWTPQILAPNPETLEPIQITEAQFKIIADAGARTVTLRVPKD
                     VFGEGDPATWGYTAVVLGQEGYPASGVWRVRDVEAAVAQWRFGGAPADTNHTRIIDMI
                     WPSESDITQAEMLSNYPPSTTSAGELTADDFAQIRLLLAK"
     gene            complement(50442..52595)
                     /locus_tag="PKL11_00245"
     CDS             complement(50442..52595)
                     /locus_tag="PKL11_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12052.1"
                     /translation="MTAPSLKPRRFKIHFRWWKRSYSIALVTLLALALRLWAAWQLPL
                     DADEPVYMRAASDYADLMARGDWSGIVNYQGNAEHPPLVKLLYSIPFQLFDPPFGESP
                     ELLFNRIVSAVLGTIAVFLLSLIDPLAGLFLAFHSMAIKYTSEVYLEALPLAAIIGSV
                     LALERASKGPRRWYWLSAVLLGIAIAGKMTYGMIVFVLVYFWLSKRPIPWKQLLLYGG
                     VALVSFWAFNPSLWHDPLSRLQQSLFFHAGYTQSADVLRANYPWYQPVIWIITTVPWH
                     PNVFFFLTSDDIIFWLSIAGFGLALKQRPWTAVWLASILAILLVWPTKWPQYTVILAP
                     AMSLIASGAVRWFIDWARKKDSYWDYLSEMLPRPPKLFWILLFGLVIVLLTGKVVYEV
                     QMAVLRRGWVQMNSTGTPLPSNEIHDLKTTPDGRVALATTNGAAIWTPNEDAPWGQAG
                     QWYVPANSGIMDRNVLSLLQDSRGTWWFGTEQGLSSFDGQTWTSFTASDIRLENAQIN
                     ALDEDSLGQIWVGTLSGASVWDGQTWTAYTAAPGGLLDTSIYSLEIQPTPNGDVIWFG
                     GGLGISRLDWQTQSWQSDDFHDLGLGWAGIADLYLDSKGQMWAGTLGSGLARWDGTGW
                     TFYRTSNSDIPYNYVQAITETDPGIYWVGVGLPTEPGGLLVRFDGQNWKRYNEHNSGF
                     AGSEPLALAEDSIGRLWIGTAAGGLQIYTLQTEEE"
     gene            complement(52592..54250)
                     /locus_tag="PKL11_00250"
     CDS             complement(52592..54250)
                     /locus_tag="PKL11_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ12053.1"
                     /translation="MHKKLVAFLALVVLLSSLLSSCVSPQSQTPASNVVALKQTAMPD
                     NWWNDAVFYEIFVRSFYDSNGDGIGDFNGLTEKLDYLNDGDPATTSDLGITGLWLMPI
                     QVSPSYHGYDITDYYTVNPDYGTMDDFRRFLDAAHARGIHVVIDLVLNHTSEDHPWFE
                     QSKDPASPYRDWYIWSEQDPGYLGPWSQDVWHFSAGAGYYYGIFWSGMPDLNYNNPDV
                     ATEMRNVATYWLSDVGVDGFRVDAARHLIEDGQTQSNTDATHAWFKDFYTLVKTENPE
                     AMTVAEVWDTLFAAVPYVKNDEFDLVFGFELASAYMGGVNGADARQIVDALSFNYGTF
                     PPGEIAPFLTNHDMNRVMSEFAGDEDKARIAAVLLLTSPGVPFIYYGEEIGMIGTKPD
                     EEIRTPMQWSAESGAGFTAGSAWISPKKDYVDKNVTAQLSASDSLLAHYRNLITIRSQ
                     HEALRKGQYIKVTTSDNRVLAAIYATENETILLLINLSPDKIKQVELTSNAEFTPGNY
                     HLADLLGEGRLSNLKIPASGELSGYQPIASLEPYAAYIAVLTQK"
     gene            complement(54283..56058)
                     /locus_tag="PKL11_00255"
     CDS             complement(54283..56058)
                     /locus_tag="PKL11_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ12054.1"
                     /translation="MSDFIFGTLATDALRRQHVDKLRSGITHLQQRSPRHPLPGQTIQ
                     LHLTSGPLLPGSRAWAYWTTDGSDPIGVQGVAFNGHALEMQLSQEEWDTVLWGYIRHF
                     TVELPGQRDDTLVRYRLSCYSPAQGEIFADNGAYYAFYVETAGPPAWTQSAVIYQVFP
                     DRFSPGSHQSWQRPASLGGFFGGTLSAILDKLDYISELGCNTIWLNPIFPSPSHHGYD
                     ATDLFTVEPRLGTLQDFTALLAAAHQSGIRILLDFVPNHWSHLHPTFQNAIADANSPY
                     HNWYTFRHWPDEYETFFGVKSLPQINLRNPAARQHLLEAAKYWLDLGVDGYRLDYAIG
                     PAPDFWADFRRVTRAANPQSWTFGEVVDPPDVQIAFEGQLDGCLDFVLLEGLRRALAY
                     GSWDGVEFGRFLERHTAFFPADFSRPSFLDNHDMNRFLWAAQGDKRRLRLAALCQFTL
                     PGQPVIYYGTEVGLSQQRDVRQGERGVPEESRLPMLWGQEQDLALLEFYRSLIALRQN
                     QLPLQIGSLQIIQSQPGLLVYARQHDNDRIGVALNLTAESMQVNLQLPVRKTLLTSEY
                     GVTVTASDHHPRVIFPPLAGVIWQI"
     gene            complement(56058..56903)
                     /locus_tag="PKL11_00260"
     CDS             complement(56058..56903)
                     /locus_tag="PKL11_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ12055.1"
                     /translation="MSRKPSVWMRILQYAVLIGFAIFAFYPVWFAILASGRLGDRLYT
                     LNLAGMFFPTEWTFENYRVMLFERPFLTWLTNSLKVAVAATIFSLIISTSAAFALSRF
                     RFRGREAFLILLLALSTFPGLLSLVAIAQLLTAVGLYGKHLGLILAYTTGTLVFCTWN
                     LKGYFDTIPIDLEEAAMLDGCGPVEAFTLIALPLARPALAVTAILAFMGSWGDFVFAG
                     VLVPAPDSLKLAVPALYSLANSISVPWGYFAAGAVIVVLPTLMVYLALQRYFESGLTV
                     GGVKG"
     gene            complement(56903..57922)
                     /locus_tag="PKL11_00265"
     CDS             complement(56903..57922)
                     /locus_tag="PKL11_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ12056.1"
                     /translation="MFRDTAPVGLGEPYRIADPPFKNYTDLLGKLFAPPALISLGKML
                     VCLVPLIIANAVNKRFAKEVTPPLASGWVWLMAIISAVILGFVLDFFAALATLMETGD
                     FLVVVLRTVMFVAIRVPLTFLVGLVLALILNSQHLPGRTFFRVVLFIPWGASAVAILI
                     ALVWQFFFRDQGVINQLLLAVFNYKGPVWLNSPVWATVVIVLADIWFSYPFFMVSILG
                     ALQSVPNELYEAADVDGASPWQQLMGITLPLIRPAIVPALVLTSITAFQMFGTAFAIT
                     GGGPQLGADKPGATEYVMVYAYRQIYQVSNYGRATAFAVLIFIMLFLATLYSLRITKI
                     TKGAR"
     gene            complement(58195..59418)
                     /locus_tag="PKL11_00270"
     CDS             complement(58195..59418)
                     /locus_tag="PKL11_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ12057.1"
                     /translation="MSKKLFPVISILLIVAFALAACTPKPTEVKNVTITIWHQWDGKY
                     LEAIQQAFADYTAAHPNVTIDLSKPEDVANALNVAIPAGEGPDIIGWANDKIGEQALK
                     GNIVALNDYGITQDFLTSTYEPAGVNGVVWSGKIWALPESQEGIALIYNKAIVTDEYL
                     PTDPMNFDDLYAKAEKFQEDTGNVLVCNQAFGGSDAYHMSPIFFGFGVPSYIDETGKA
                     NLNTPEAVAAAEWLLKFKDVSLAENSYDICFAALKEGKVGMWWTGPWAVAGIEESGID
                     YGILPFGRPFVGIKTLMMTKNAVDRGNQDVVIDIMKYFTGAEVQKKLSLINKTIPAAT
                     EALLDPEVQALPAVAGFGAALNLGVPMSPSPYSSAQWDPVGKATQAIWQGAATPADAM
                     AAAQTAAEEAVAQMK"
     gene            59710..60522
                     /locus_tag="PKL11_00275"
     CDS             59710..60522
                     /locus_tag="PKL11_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HNZ12058.1"
                     /translation="MNDQWRLVQTQPSDGAWNMAVDEAILESVSRGDSLPTLRLYGWA
                     PPCLSLGYAQPIADVDREALKARGWGLVRRPTGGRAILHTDELTYAVIAPVSEPRVAG
                     SVLESYRRLSRALLLALQKLGLQPNADREYPQSNVGGPVCFETPSNYEITISGKKLIG
                     SAQARRREGVLQHGSLPLHGDLKRITQGLMFVDEAARLQAGERLLEHATTVETGLGRV
                     IGWEQAASAFIEGFREALAIDFVPSELTTNERVRADELTRTQYGNDSWNARI"
     gene            complement(60523..61215)
                     /locus_tag="PKL11_00280"
     CDS             complement(60523..61215)
                     /locus_tag="PKL11_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNZ12059.1"
                     /translation="MSGSQGKSGFIGAILVLVLILGALFLGLTWFIRSATDQALSPLN
                     EANDQLKTQVSNLLNPTPTILPDPVTIITEVRSLARLETIEYTVEKVITAEIGQGELA
                     FLFGDKLLFVAHGSVIAGIDLEKLKAEDIWLEGETLYVKLPPAEIFVATLDNEKSYVY
                     DRETGLFTHGATDLETQARQVAEAEIYKAAIEDGILEQAQQNAENYMTRLLRSLGYNT
                     VIFVAPTPTPAP"
     gene            61328..62308
                     /locus_tag="PKL11_00285"
     CDS             61328..62308
                     /locus_tag="PKL11_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNZ12060.1"
                     /translation="MALVACTKQPTNDRPTATAQVSPTPTATPAPLGSVENPVVMGFV
                     EPTTGSGNYPPEQVELAQAITVNTGFTVSSRVYANWEELLEAMQDGEVHLAWMQPLTY
                     LYASEEGIAEIGLLSNHFGLYYYGVQYLANIESGFTVYYDAVSQQNTADAATALAQFG
                     GRRPCWIEPKSISGYVVPASLLADTEVPTLDGTYVQTHTAAVRALYIKGICDFAATFA
                     ISGDPRTASGVLDDLPDALNRVVIIWRTDASIPSLGLAYAQSLPEQFRQAINLTLTDL
                     VKTDTGKAILAGANDGYDIQDLRVVDDSIYDSLRENVSALSYDLENALGR"
     gene            62408..63049
                     /locus_tag="PKL11_00290"
     CDS             62408..63049
                     /locus_tag="PKL11_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNZ12061.1"
                     /translation="MTFKLVLYLILAGMIILGLPRIIALMYAKPRLYTSVDVPVYQAA
                     IVFGAGLQRDGTPSPVLRDRVATAADLYFEGKVEKLLMSGDNRFVNYNEPGAMQDYAI
                     SLGVPAEDIVLDYAGRRTYDTCYRAHAIFGLDSAVLVTQGYHLPRALILCNNLGLESV
                     GVAADRRVYHGRSYLIWQIRELPATLVALWEAWVTRPLPVLGDYEPIFPDSNP"
     gene            63074..63649
                     /locus_tag="PKL11_00295"
     CDS             63074..63649
                     /locus_tag="PKL11_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNZ12062.1"
                     /translation="MNRDEALILVKEYVKNEGLINHMRSVEAAMRFYAEQMGQDAELW
                     GLAGLLHDFDWEIHPTAEQHPLDGEPILRARDVPEDIIQAILSHADHTGLPRTTLMQK
                     ALYACDEVTGLITAVALVRPSRSLYDLTASSVKKKWKDRAFAAGANREEMEKGAAEFG
                     VDLWSHVDNVILAMRKIAPEIGLVGTLQPEA"
     gene            complement(63661..66456)
                     /gene="gyrA"
                     /locus_tag="PKL11_00300"
     CDS             complement(63661..66456)
                     /gene="gyrA"
                     /locus_tag="PKL11_00300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ12063.1"
                     /translation="MEVGLVRKIDIDHEMQQSYLDYAMSVIVSRALPDARDGLKPVQR
                     RILYAMYDMGLRPDSAYKKSARIVGEVLGKYHPHGDVAVYEAMARLAQPFSMRYPLVD
                     GQGNFGSVDGDPPAAMRYTEARLTAYAIDLLAQLDRNTVDFTRNFDDTLSEPAVLPAA
                     IPNLLVNGASGIAVGMATNIPPHNLGEVVDALIFMLRQWEQMEDISVADLVKFIQGPD
                     LPTGGIILQDHDRNDLLSAYGSGRGRVILRGRVHLEEMSRGRDRIIITELPYQVNKSA
                     LIERIAELAREGDLEGIADLRDESDRQGMRVVIELKAGADEQKILRELYRRTPLETTL
                     SLTLLALVDGEPRLLTLKQALRVYIEHRLEVVRRRSEYDLAKAKQRAHILEGLRIAIN
                     NLDEIITLIRNAPDVETAHARLVKKFKLSDVQAQAILDLMLRRLAALERKKIEQEYKD
                     LQALIKELESLLKSPKKMRGAVETELMAMRQAYGDRRRTQIVSLADGEVAADKLTTTD
                     LTPAEVVWVGVTEDGVIARTSGDELPRISGRQAARWLLRTSTHHTLYLVAQTGRAAAV
                     AVHALPEAEKFADGVKLNKVSPFDADDALAAIFSIPERLEPGQERYVVTVTRLGMIKK
                     SLVSDLSGPSAQGFVLSRVNAGDELGWAFVTDGSSEILLVTALGMLIRFSEQEVRPMG
                     LVAAGVNGIKLETKDRVAAAERLSGEGEILFVSSEGLGWRIEKDGVPLQGRYGQGVLA
                     CKLTGSNRLVGAIFGKRTQTALVHFQQAAARLTRLDEVPLGKRLMKGQDLAPIKAGDA
                     VLNLTAISDGLALWESEIKPTRKPRRTAEKGSAPAEKNAPAKKNPPASPASKKSTSTA
                     KPKTTSKAKPAASSKPTATPKTPVAKPTAKPAAKPKPVSKADTTTSKPASKKQKPASA
                     AGDAKKLKPAPTSRRKP"
     gene            complement(66572..70801)
                     /gene="rpoC"
                     /locus_tag="PKL11_00305"
     CDS             complement(66572..70801)
                     /gene="rpoC"
                     /locus_tag="PKL11_00305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ12064.1"
                     /translation="METKSLVALRINLASPETIRSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTRDYQCYCGKYKNPRYKGIVCDKCGVEVTRSAVRRERMGHIDLATPVAH
                     IWYTRRVPSYLGLLLDISRRNLDRVLYFAQYIVTYVDEDARQKALRRLEDEISVSERE
                     QATQVNARILEVKQSRDRKIAELQQRKTEMEERRDEQIAARLEPVIQEGQSLERTLTD
                     KQGQTLRSALVFSAASQEIVGVGETITAQHMTKVQQIVKERLNQLESEFRDEFARELE
                     QIKMGIMEVRAQADEEMETLRNQLEDQSSVSQNQNSHFRDELQELRPFTFLSESRYRE
                     LKSRWGQVFRADMGAEAFYDILHRLDLDKLSEELWHEARTSKSKQKRKKATTRLKVVE
                     SFRRSGNSPEWMIMTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLVDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPTLKLYQCGLPKAMALELYRPFVISRLVSHNYAANVKGARR
                     FIERNRPEVWEVLEEVIHERPVLLNRAPTLHRLGIQAFEPVLIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSEKAVWEARNLMLASRNLLKPADGEPIISPSKDMVLGVYYLTM
                     RNDLPHSGDGRVFADIDEVEMAYQLSQVDIHANIRVMMETNLDDNGDRLPSPIRKLIE
                     TTVGRVLFNRILPPEIRFVNDVLDKGGVKDLIAEVYEYCGEEVTTEVADNVKAIGFEY
                     AMRSGSTLSVSDITIPAEKDEILTQAQRDVENVERSFRRGLLTEQERDEREIEIWQQT
                     TKDVASAVRRNMDPNGNLATMANSGATKGGFGPISQLAGMRGLMADPAGRIIRLPIQS
                     NFRQGLTALEYFISTHGARKGLADTALRTADAGYLTRRLVDIAQDMIVNEEDCGTHDG
                     LWIRKSDDIAGQSMSVRLFGRLTAARIVDPLTGEVLAERDEMLGHDLIRRIRQAGIDE
                     VKVRSPLTCELVHGLCAKCYGMDLGRGRMVELGSAVGIVAAESIGEPGTQLTLRTFHT
                     GGVAAGSDITTGLPRVEELFEARRAPKGEAVITSIAGVVAVIVNERYSDQRMVRVDHS
                     EMVSDEYDIPEGWEIRVKDEDAISVGDELATQGEAVVKAQHTGRVRLEDRKVIVSYEQ
                     RETEEYEIPTTSRLLIKDGEHVEAGQALTEGSLNPHTVLRIKGREACQMYLMTEIQKV
                     YRSQGQNIHDKHFEVILRKMVGRVQVIRPGDSGYLPSDLVDRLEIRRVNEQLIAEGKQ
                     PARYAEILLGVTKASLSTDSFLSAASFQHTIKVLAGAAIASTEDPLFGLKENVIIGKL
                     IPAGTGFVYGRFRTQAAEEGPQADAELPEEA"
     gene            complement(70948..74265)
                     /locus_tag="PKL11_00310"
     CDS             complement(70948..74265)
                     /locus_tag="PKL11_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12065.1"
                     /translation="MTTLQLGNLNIIWPGGLLGWLGWFALLAVLFWGVRLAWTPGAFK
                     NLRWGLLAAFIALTPFATFLMVAQLSGSGIPLPNMPGDAAAPVLIMFFALPWVLAAGF
                     LGVIPASLVGLISGIILSLWGTHNLFTPIEITGLALVFAMVIRQPYRTTFYRLLRQPL
                     IAVLAVGVIFLPFYLLSSFFTVNGSIAVRMDYALTQSWLSFLARIGELLIAGIVAQIL
                     LLFPAMGWYRPAVLQPSPTESSLSWRFFFSIMPLVIITALVLTLGDWWVAGSAARQMI
                     NGRMSSAAEVAAKSLPYFLEAGQTLVQNYATPDLIPLSPQTQADRLADQVRLSPYFRQ
                     MVLVDSTGAILSGYPESRVANIKLAPEELMAVQLALQGVPIQTYAIPAYTQPADQNPI
                     SAQISFISPIQSETGQMIGVLIGRTDLVSNPYTQPAIEAMQTIAAEGGEGMILDENQR
                     ILYDTMQSPELVMSSYIGQPQTEAAFFEDVSASGTRQLVYYQPGEGRPWAIVLTMPAE
                     GANQLALKIAIPLLLILALFVVVAYSTLRLTLRSITGSLGLMANQSVKIAQGHLDNPM
                     KVRGVDEIGRLGTSFEQMRISLKARLEELNRLLVVSQGVANLEVGEAVSAILEAALGP
                     DSNSARIVLIRDVMMETHESPWVSFGAGPATETYAYLDKQIFDLMRHQDLLPIPNTLR
                     VRRVAPEPGQLFPGALIALPLYYENYYYGVLWVGYESPHNFSESEVRFLSTLSSEAAV
                     AAVNARLYATSEIGRQRLEAVLASTPEPVLVFDEKDRLFLVNPAVLQVPGLIASAMPG
                     QPASEVVLMPELLDLLQTPPESGLVSRELALQNGRIYYASVSPVIADGRQVGKICMLR
                     DITHFKELDTLKSDFVSTVSHDLRSPLTLMRGYATMLQMVGELNEQQQGYVKKMIGGV
                     DNMTRLVNNLLDLGRIEAGIDLRLENIRANEIVSQVVALLQPQAAQKNIQIVSELPED
                     GPVVIEADSALLQQSIYNLVENAIKYTPVGGRVRVHLQARAETILFSVIDTGIGIAPL
                     DLPRLFEKFYRSGRREAYQQHGTGLGLAIVKSIADRHGGRVWVESQLGKGSAFFFEIP
                     REQPAAKKPKQNPKIEN"
     gene            complement(74340..75410)
                     /locus_tag="PKL11_00315"
     CDS             complement(74340..75410)
                     /locus_tag="PKL11_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12066.1"
                     /translation="MPPKILIIEDEDQIRKNIAILLKNNAYEPLEAANGEEGLRLVWE
                     NAPDLIICDIMMPGLEGYEVLKILSQDPNTSAIPFIFLTAKDKRSEIRQGMELGSDDY
                     ITKPYNNAELLTAVQTRLQKRRLMKDLIDARFSDMVRQRSFSVPGYLLPPLSVLLGYS
                     DLLANSTEPLEGSQIRQIGREIQNSAQHIARVLENYRIFSELSLVLANPEQLSIYRQC
                     QCLEAHNLIWELVAVKTLSGDRKEDIKIESESVNLAIQESHFQRVVEELLDRALRLSL
                     PHSQITIVGQKIGWEYRVQISFYGRPALNRNDLDPAADLGLMVVTQLMELYRGRLTIE
                     LTEELSQLSIYLPITQLTETQP"
     gene            complement(75414..76406)
                     /locus_tag="PKL11_00320"
     CDS             complement(75414..76406)
                     /locus_tag="PKL11_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ12067.1"
                     /translation="MTPVWSILSGNAPLNAETDTWDALILSVDSDAPSAVQLIQSVRS
                     RGLYLPILAVGESESIAFSNTLLDAGANRYIPSSLINQYLALALTAALRESQTDHEIA
                     SVKARLTSAVSHEFRNPLTIALSSTELLIAYGESWSSDGRMSQLEQIHTAVQRLNQIV
                     SDVLDLQLLDGTLVAKASPCEDVITICRRAIADVSAWDQNNHPINFALPAPDFTEQAP
                     PDLFYLVVLHLLLNAIRFSVPGKPVLVELSSGLNCYHLTITDQGSGIPADEIPWVFGR
                     FCRGRNVRKIPGVGLGLTIARLAANSLGGDITLDSHLNSGTRAVVTFPTQLTGR"
     gene            complement(76486..77181)
                     /gene="cmk"
                     /locus_tag="PKL11_00325"
     CDS             complement(76486..77181)
                     /gene="cmk"
                     /locus_tag="PKL11_00325"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNZ12068.1"
                     /translation="MNYPKTIAIDGPAASGKSTLGERLASRLGYLYFDTGVMYRAVTW
                     AALRSLKTVDDEAAVTAIANQIQIDVSAPTKTDGRKADILLDGEDITWDIRRPEVDAN
                     VSQVSAYSGVRSAMTAQQRRIGLRGCVVMVGRDIGTVVLPEADVKIYLVASVEERARR
                     RTDEVLARGERASYATILESMRRRDQIDSTRAVAPLRPADDAVILDSDGLDSDAVFAR
                     ACEIVEQCATGDG"
     gene            complement(77195..78886)
                     /locus_tag="PKL11_00330"
     CDS             complement(77195..78886)
                     /locus_tag="PKL11_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12069.1"
                     /translation="MKNRPITIEHILFALAFLLALVSRLWALAATPLGDTEAALALQA
                     AHLFSDRTAAIDPQPAYILMTSVLFAIFQATDFTARLIPALAGSLLVLSPLLFRRQLG
                     RWSAILLAFGLALDPGLTAVSRQAGGMMIGLACTVLALGYYIRRSSAAAGICLALALL
                     SGPNFLPGWLALAIAVLVFGRSSIAASSDDAPAPFSWRNALIWAAGTLIVVGTLLFLQ
                     PRGLSAAAASLGTYFQGWGRASGISLGQLPLALIFYEFLPLVLGLVGGFRSLFKAEPI
                     DRFLFFWFGIALLLALVYPSRQVIDLSWSLLPLWVLAARQAIRWAGIVREHRFISAGQ
                     AALVAALITVVLVNLAALVTQTTLSEAQVFARWAQLAAALILLAILTLLVAGGWSIPT
                     ALNGLTWGVAIVAVVLTVAAMWNAAGRGNYPDRELWRVDAYPAEQDLMLKSLSEISDW
                     AQKNRQPLDLVVIQESPSLRWALKDYPQAEYDSVLPVDARPSTVITDQTNTLQLTAPY
                     SGQDFTIYTAPEWPAMVAREWLEWGFYRRAPVYTTYAILWVRTDLFPGAAINPTP"
     gene            complement(78899..>79854)
                     /locus_tag="PKL11_00335"
     CDS             complement(78899..>79854)
                     /locus_tag="PKL11_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNZ12070.1"
                     /translation="MVFWASMINSLFGASDSGALLGAALQRRITYVGTWLPLTTIVVL
                     AWGLMSALKRRTASEESTSQQITPVQAFAALLALLGAGLALFPEFFFLRDNFGNRMNT
                     IFKFYFQTWIIWSLVAAFASAVLWEALSGFRAVIFSAVWSIVLLMSLTYPVTMLPYYT
                     GNFKVTDNYTLDGTAHIERSQPDEMAAIRWLRDAPYGVVAEAVGGAYSSYARISVNTG
                     LPTVLGWDNHEGQWRGSRDEIGSRTPDMQQLYVSSDWEAVKAILVRYNIRYVYVGPLE
                     RNTYPVNDNLFQTYLKPVFVTNTVMIYEVPQLEIVPLQTTP"
BASE COUNT        19808 a        20042 c        22434 g        17570 t
ORIGIN      
        1 ggcgggtaca cctcggcatc agtatttcag agcgtctggt gctgcgctaa gagataattc
       61 agaaaggatt attccatgat caaaattgtc gctgatacca cttcttgcat tgctccagaa
      121 aaagcagcag agctagggat cgcctatatt ccacagatta ttatttttgg ggacgactcg
      181 taccgcgatg acaatgagat ggacagcgct acatttctta agagattgcg cgcttcatcc
      241 agcctgccaa aaacagcagc accgccgccg gtgctttacc aacccatcta tcaggaattt
      301 gcgcagcccg gcaatacaat ttttgtgatt tgcccctcgg cagaagtcag tggaaccgtg
      361 cgcagtgcaa ctgttgcagc gcaggagttc cccaaagctg acatccggat tattgacacc
      421 cgctcaattg ggtcgggcct gggcagtgtg gtacttcagg cggttgaatg ggtgaaagaa
      481 ggggtagatg cggcaactct tgaatcaaat ctgcgcgata tgatcagccg tgaacatgtg
      541 tactttatgg tagacacgct tgaatatttg tataaaggcg gccgaatcgg cggtgcccaa
      601 atgctatttg gcagtctgtt gcaggttaag ccaattttga ccgtcaaaga cggacgcact
      661 gaaccggttg agagccagcg gacccataaa cgggcgctgg agcgcgtgaa gcagatggtt
      721 tattcagact gcccgcatgg gccacaagcc catattacca ttatgcatgg ggatgcgatt
      781 gaagaagcca gcaaactggc agatgaattg aaagccaacc tggggctaac cgaggtgccg
      841 acctatcagc ttccgccagc tattatggtg catgccggac cgggaacttt ggcaattagt
      901 tattttgtag aaaaagcgag ctgatcgctg gaagactgga atttgtcgtt tctttttgaa
      961 gattcaaccc tgctggcaat caacaaacca gcagggttgc gttctattgc tgatggatat
     1021 gatgccagtc tgccgagtgt gagggctctt ttgagtaaac gttatgggcc cgtctggatt
     1081 atccaccgac tggataaaga taccagtggg gtcttattgc tggcccggaa tgctgaagcg
     1141 catcggcaga tgagtattca atttgaaaat cgtcaggttc acaaaaccta tcatgccgtt
     1201 gtgcggggga ttcctccctg gcgagagata accagcaagg aatccttgcg agtcaacggc
     1261 gatcgaaagc acagaaccat cattgacccg gctcttggca gggcagcaaa aacagatttt
     1321 gcggtattgg aacaatttgg ggattgcgcg ttgatagcgg caaggccgcg cactggctat
     1381 acgcatcaaa ttagagcgca cctcagtgcg ctaggctacc ccattttgtc agacgatttg
     1441 tacgggataa gaggtgcccc gctgcccggg ccagatgaac gcatatcacg attggcgctt
     1501 cacgcagccg gtatcgaatt ctcacaccct caaacaggtc ggtttatctc aattgaggcg
     1561 ccctatccgg ctgattttgc gcatctgcta atgacgctaa agcaataata aaaaacagcg
     1621 ggcggcttta tcaaccgccc gctgttaatg taaatcttgt tacctcgggc taaagcgccg
     1681 gggacgattt cttgctttgc agaatagccc aaacaatcgc agcggtcagc accagggcga
     1741 gagcccagcc accccaaccc agggtttggt gctgaacaat gatcggagcc gcaagcagag
     1801 agaccagatt gacgacttta atcatcgggt tcaatgccgg gccggctgtg tctttgagtg
     1861 ggtcgccaac ggtgtcgccc acaacgccag ccttgtggcg ctcggagcct ttgccagtat
     1921 tggcagctag gtcacgcggc tcatcttcaa tagccttttt ggcattgtcc caggcgccgc
     1981 cggcattggc cataaagaca gccaacaact gaccgctgag gataacgccg gcaaggaagc
     2041 cgccgagggc ctcaacctgg agaaccaggc caacgaccaa gggcagtaca accgagatga
     2101 ttgccagagg aatcagttcc ttctgggctg agcgagtgga aatacccact acacgcgcat
     2161 aatcaggttt tttggtacct tcccaaacag ctttatcgcg gaattgtgcg cggacttcct
     2221 ggacaacctg agcagcagca cgagttactg cccgaattga caatgaagag aagagccagg
     2281 gcaaagcgcc accgagcaag aagccgataa agacttttgt gctggagatg tcgattgccg
     2341 tcagggttcc aggggcgact ttgttgatat cagccatgaa ggaagcaaac aaactgacgg
     2401 cagcgatgac ggccgaggca atagcaacac ccttggtaat ggctttggtg gtattgccca
     2461 cagcgtccag gtcagccata atctggcgcg ccttgactgt ttcagggtcg gtcataccat
     2521 gccaggccat ttcggcgatt ccattggcat tatcagagat ggggccataa gaatccatcg
     2581 caacgttgtt gcccgtgtgg ctgagcatac caataccaat catggcaacg gcgtaaagta
     2641 cataattagc gccatcggct tcgtatagga ccagcgacag tacaaaggag atcacaatca
     2701 caaccaacgc ccaaaccgag gattccatac caaccgacag accggacaga atggtggtgg
     2761 cagggcctgt atcagtcgat ttgacaatat cctgaacagg agctttcttg gtcgccgtga
     2821 agtaggaggt cagaggattg aaggcaaccg ccagacccac gccaatggtg gtcagcatgc
     2881 ccatgcgcca gtcaccgaca taagtgatcg caagaatgaa ggcccagaga atagtattga
     2941 acgaggatac ctcgtaagat ctgaacatcg cttcttcggc atcgtgggct cgcaggaact
     3001 tgatagggaa cttttcccaa ataggaactg tgaatgttcc caaaattgag ctcagtacgc
     3061 caattgcgcg aataatcaac gggtaaacaa tccaaacgat gctacctgtg ctttcataaa
     3121 gcaccagacc gaggatcagg gcagagacaa tggtaacttc gtagctctca aagatatcgg
     3181 cagccatacc agcgcagtcg cctacattat cgccaaccag gtcagcaact acagcagcgt
     3241 tacgcggatc atcttcgggg atatcttttt ctactttacc caccaggtct gcgccaacat
     3301 ctgcagcctt ggtataaata ccgccgccaa cgcgcatgaa aagagcaagc agggtgccgc
     3361 caaagccgaa accaagcaag gcatctggag ctgcggtgcc gaaaataatg aagatgaccg
     3421 taccgccaaa cagtcccagg ccatctgtca gcatgcccgt aattgtaccg gcatagtagg
     3481 caatcgagag agactcattg aagtcgcggc gagcggcaga ggcagaacgg acactggcct
     3541 gaatagccat gcgcatgccg agttgaccca ccgttaagga gaagaaggcg cccatgaaga
     3601 acgcaatggt gcggccgaca gagacagcga taacagcatt atcgccgaaa cgatcaacag
     3661 cctctttagt gggttctaca acgtagacac tcagaaacag tgccacgccg agcaagagga
     3721 ttgccgggag gattgttttc aactggcggc taaggtagct atttgcgcca actcgaattg
     3781 cgtcccagac ttcttgcatc tttggtgtgc ccttatcttt cttgagcacg ttgccccgca
     3841 gcagccatgc atacagcagg ctgataaagg ccacaagcag cacacataga atagcaatct
     3901 gctcaaatgg tgtcttgagg ccgcgctctg caagaccaaa tatgtccata cttcctccaa
     3961 attgataggg atttaaagag actagattat tttacttaaa tgtgaatgat tgtcaattga
     4021 agatgggcga gaatttattc agccgaaaga ttagtattag attagaattt atcaacctta
     4081 ttgactttag ggggagcgca attgtatagt gtattgcggc aaacaattgg cagcaagtgg
     4141 tctgacaatt gctccaaagc aatatgggcg ggctgctttt tataataacc ccgattcggt
     4201 aactgaggtg cgaaggatct gcttgatcag agccaatcgg aaacagttga gagattgtca
     4261 ctatttgagt cagtgccatt ttgtgccaaa tggcctactt gatttttgct tgaaaaacgt
     4321 gtattctgga taacaggact aaaacggggc acggaaatcc attccaataa gaggaattgc
     4381 gcaaactgta tggagaaccc tttcattaac gtagtcgacg aagaagacga agaatatcca
     4441 gcgatagccc gcctgattga actggggcgg caaaaaactt ttgtaacaat agacgatatt
     4501 ctgcacttct ttccagacgc agagcaagac gttgaacaac tcgaagaggc tttcgccgcg
     4561 ctgatgagcg ccggcatccc ttatcttgag gatgaagcgc cgctggctga gccatctgaa
     4621 gatgagttga tccttgaaga agagcccaac gaacgagcgc ccgaagttgc aattgacgat
     4681 cttgaaaaca ttgataccga cgacactatt gggctatatc ttaaagaagt cagccgtgtt
     4741 ccgctactaa cagcggaaga agaagtggag cttgctcaac ggattgaacg cgggcgcatg
     4801 gcccgcgaag aactggcgcg gggtaacgtt aatacccgcc gccggttgga actgcgcaag
     4861 ctaattgaag acggttgggc ggcgcgcgag cacctgatta cggctaactc tcggctggtg
     4921 atcagtgtgg cgaagaagta tatggggcgc ggtgttcctt ttctggatct gattcaggaa
     4981 ggaaatatcg gcctgattcg ggcaaccaaa aaatttgatt accgccgcgg acacaagttt
     5041 tccacttatg ccacctggtg gatccggcag gcagtgaccc gcgcgattgc cgaccaggga
     5101 cgtacaattc gggtgccggt gcatatgggc gaccaaatta acaagctcct gcgagtgcag
     5161 catcaattga cccagcgcct ggggcgcgaa ccaactgtgg acgaactagc acactcgctg
     5221 gatgttcccc ctaaaaaagt ggagaacatg atccaggtag cacggcgacc actttcactg
     5281 gaaacaccaa cagacgacga agaggattct gtgctgggtg atttcattga ggacgacgag
     5341 gcgccgccgc ccgatgacac cgtgacctac aacctgctgc gggagcatct agaagaagtg
     5401 ttgaactcgc tgcctccgcg cgaggtgaga attttacagt tgcgctatgg gctgctggac
     5461 ggacaggctt atacgctgga agaagttggg cgtaagatgg gcgtcacccg tgagcgcgtc
     5521 cggcagattg aggcacaagc actgagccga ctgcgccatc ccaccattcg gcgaaaattg
     5581 cgcgactatt tgggcgaata agtcagactc ttttataaaa acaacggcaa ggagcagtta
     5641 aacagccttg ccgtttgtat tttaaaacag ggcaagcaaa aatgcttgca atattattaa
     5701 atctgatgca tactaaaacg tggaggacct ctcttcatgg ccaaaatatc tcttcgcgca
     5761 taccaacgtg atatagaaaa cctgattgag cgtggtcaga cagacgaagc catcgcccat
     5821 tgcaaatata ttcttcgtta ttatcccaag catattgaga cgtatcgcct gctgggaaag
     5881 gcctacattg aaagccagcg ctataccgag gcgtcggata ttttgcagcg cgtcctgtcg
     5941 gttattccag atgatctggt ggcgcaaatt gggatgagca ttattcgaga ggatgaaggc
     6001 aatttagacg cttccatcta tcacatggag cgcgcctttg aaatccagcc atcgaatgtt
     6061 gctgtgcagg aagaattgaa gcggctttat gggcgccggg atggcgttga accgtccaag
     6121 attcgtttga cccgaggggc cttggtgcgg atgtatgccc gtggagagct ccaccaacag
     6181 gccattgggg aaattcgcgc cgcgcttgct gaagagccca accggatcga tttggaagtc
     6241 attttagcaa aaatggcagt tttatcgggg aaaaaagtag aagctgtaga aatctgtggg
     6301 cgggtgctgg caaaattacc atattgcgct gaagcgaatc gcctgctggc agaaatattg
     6361 cccgaaacca gccgggcaga agatgccaaa atttatcagc agcgcctgat tgacctggat
     6421 ccatacctgg gatatatttc tccaacaacg ccgaccacca atgatgtccc tgaaaatgcg
     6481 gttgcactgg aacggttaga ctggcagccc ggcgagaccc agcccgacac gcgcccgcag
     6541 tgggcgcagg ctgtgggcgc cagttgggat acgcgggaag aggaagcccc tgctgcggat
     6601 tggctatctg cggctatgcc gccggctgag cagccctcac ctgctgcaca acccagtgcg
     6661 ccagataccg gaattccgga ttttctgcag gcagcaggct ggagcgtatc ggatggcgac
     6721 gcgacatcac gcccagagac gcaagtctcg ggattggagc aaggcgaaga ccttggcgcc
     6781 ggagaactgg cggcggctga aatgcccgat tggctgcaat ccattgcgcc gccgcaaacg
     6841 ggtgcgcctg cagaatcggg caccctggaa accgaagaag acagcgcacg ctggctggat
     6901 caaatgatgg cagcccaacc gccggcagaa tcggtggaat ctattgcgcc aatagacgcg
     6961 gctgctgtac cgcctgttga tttatctgca atggatatgc ccgattggct ttctggactg
     7021 accggagaaa ccccggctga aaacgcgcca tcaccggagc cagttgttgg agaagaacta
     7081 gctgctcccg ctgacacagt tccggattgg ctgtcaggtc ttacgggtga aaccccaaca
     7141 accgaagtac ccgaagcaga agccgaactt ttcgcgattg aagccgaggc tcctgcagaa
     7201 ccggccgaag cgctgccaga ttggttgtca ggccttacgg gcgaggctcc ggcgtttgaa
     7261 gcgcccgagg tagaagccga gcttcccgcg attgaagctg aaatcccggc agaaccggct
     7321 gaggtgctac cagattggct gtcaggcctt acgggtgaag ccccggcggc tgaagtgccc
     7381 gaagtagaag ccgagtttcc cgcgattgaa gctgaaatcc cggcagaacc ggccgaagcg
     7441 ctgccagatt ggctgtcaaa cattgcgggt gaaaccccaa taattgagac cccagagatc
     7501 aaagccgaaa ccgagatccc ggttcttgag ccagaaatgc ctgcagaacg ggcagaaact
     7561 ttaccggatt ggttatctgt gcccaccgac gagttgcccg tagaggcaga acccgctgct
     7621 ccgcagactg aggcgactcc tgagtggatg ccagaaacag cactgcccga agaagaactt
     7681 gttcgttcgg aaacagaggg agcccccacc attgaccagg cgctgccaga ttggctggct
     7741 ggaattggcg agccagtaga agaacaaccc gaaccccaag cggctgaacc cgtagcagaa
     7801 gcgccaaccg aagatgcagg ctttgcctgg ttagaggcac tggcagcccg gcaaggtgcc
     7861 gaagcaaatg agcttttggt ggcggccgaa gaccgccgag agacaccccc tgagtgggca
     7921 accgaagccg agaacgcaga aactgcccaa gctccaatag aagaactggc agagcctgtt
     7981 gctgaagcgg cgactgaatt gatcgaaacg ccagtagagg aaatcgtcga aactacggca
     8041 gtcatcagcg aactcccgac agaagcagcc cccacagccg aagaatctac aactgctgcc
     8101 agtgaagacg aggcatttgc ctggttggaa gcgctggcgg cgcggcaggg tgctgaagcc
     8161 gacgaactgc ttgtggcagc tgaagatcgg cgcgagactc cgcccgattg ggctctcgaa
     8221 gctgaaaagc cacaaacaga ggcggtagaa attcccgaac ccgaagccgt ggctgcagag
     8281 cccgtaattg aaacagcggc ggacctggtg gtcgaagcag ttgaacccga agctaaaatt
     8341 ggcactgaac cggcagccga gctgcccgcg gaggctgctg cgccgcagac tacctctacc
     8401 tcggcagagg atgaggcttt tgcatggctg gaggctctgg cggcgcgcca gggcgccgaa
     8461 gctgaagaac tacttgttgc tgaagccgat cggcgcgaga cgccgcccga ttgggcgctg
     8521 caagcagagg agccggtcgg tacgacagct tcatcagaag aacctgaatc cgctacagta
     8581 gaagataccc gcccaacccg gattagaccg gaagctgaag tttcacccac ggagattgtt
     8641 ccagaggtca ctatgcccgt gacagaagca catgcggaag tgatcgaaga acagcctgtg
     8701 gtcggcgagg tgccggcaga acccgtatcc acagcggcag cgactcccag cgaagacgag
     8761 gcatttgcct ggttagaagc gctggcagcg cgccagggcg ctgaagccga agaactgctt
     8821 gtggctgaag ccgatcggag cgaaaccccg cccgaatggg tgcaggaagc tgaagcgagt
     8881 gcgcctgaag cgctggtgga aacgccgagc gaagagatta ttgaacccgt gcaacagcta
     8941 gaagaagcgc cggagccact tggtggaatt gaaccctctg aggaaatggt tgctaaggcg
     9001 cccgagcagc ccgtggcaga gatgcccgcc tggattgcgg ctgctgttgc agaagaagcc
     9061 agccaacctg agccaccgca acttgaacct gaggcagccg agctggaacc cgcaactacc
     9121 gaagaggcga tcccggactg gctgcaagga ttggctccga ccgaggagaa gccgcaagaa
     9181 tcggtgctgg aatggctggg acgcgaggaa ggcggacagc ccatcacgcc gcccaggttt
     9241 accgagccgc aggttgttga tcaaagagaa tcacctgtcg aagaagaacc ggtcagctta
     9301 ccggcagaag aagttaaaga cgccgcagaa gttcatcgtc aggcacagga agccctgtgg
     9361 aaaggcgatc tcaaaactgc aatggcgctt tataaccggc tgattgaaaa tgggctgatg
     9421 ctagaggaaa ttattcacga tctaagagac gccctgtatc gatacccagt tgatgttgag
     9481 gtctggcaaa cgctgggcga cgcctatgca cgcagcaacc tcttacagga agctctggag
     9541 acatatacca aagctgaaga attgatccgc taacattacg gaggctattt tggaacggac
     9601 actggtttta atcaagcccg acgcagtaca acgcggctta atgggcgata ttattgtgcg
     9661 cctggagcgc cgggggttga aatgtgtggc tgccaagttt ctacaagtga gcaaagagct
     9721 tgccgaacag cattatgcag tgcacaaagg caaaccattt tacgaagggc tggtgcgata
     9781 catcacagcc agcccggtga tggcaatggt gtgggaaggg cccaacgcaa ttgcagccgt
     9841 acggcagacg atgggctcca cccgccccac cgaagctgcg ccaggtacag ttcgccacga
     9901 ctttgcactt gaaatgagcc gcaatctgac gcatgcctct gatgcaccag agaccgcagc
     9961 ccaggaaatt gctttatggt tcaaaccgga tgaaattgcg ccctgggatc ggtgtacaga
    10021 tccctggctg tttggtaaaa attagccctc ttttactaag gagagacatt ggaaagaact
    10081 gttccaacca cggcatccgg ggagattgat ctttatctcc ggacaatgta ttcattgctc
    10141 cggaccacgg cggatgtgca aatccggact ctggaagaag tgcatgccgg catcaattcg
    10201 ccgctgcatg ctggcgcccg gatggaaagc ccggatactg cggcgtttac gtacagtatg
    10261 ctcagattgc cttcctgtat gccacagatt agaacggtta ttcttggaca aagcgcggaa
    10321 ttatttgcgc gctctggcta tcccaatatt gaaaactggc agccggtatc ggcacgggca
    10381 cgccggcgac gctgctattt taaccgtcaa gatattctgg cctgttttat tgccagtcga
    10441 tctgatattg atgatgttgt gcccgcgctt acggcttacc agattgaatg gaataagctg
    10501 catgacctgc tgaaagaatg cccagaagga actattgaag atgcggcgaa aggcaattcg
    10561 gtagctttta ccaacctggc gaaagtctta agagtgaccc caaatgattt gcagcggcta
    10621 tgggttgtct ggggggcgct tgagaccgct caaaatggcg aaacaaatga tgcccgggcc
    10681 gctcgatttc ttcagcgaat tgcccaacaa aaatgtgctc tgcggctgcg cttactgggt
    10741 gggtcgctga gcgattactg gcgcgccact cgggcctggt gggataatat tgaacatcag
    10801 cagccaggtc tgctggatcg acctgtatat tttatatcca gcaatacgca cagcctgtct
    10861 aatttactgt ctggttttgg gttgacgctt caatcagcgc tagaggacta cttgagattg
    10921 cctgaaaacc tcaagctgca ggaagaatgg cagaccattc aatcggggca gttggggtca
    10981 agccgtgaga atttcctcta ttacattctc aaaaaatacc aacaatcaga gtatggacgc
    11041 gaagctgggc agcagcaaat agactgcgag aaaaatgcag gaatttaccg agtgcccagc
    11101 gaacactatt ttgatattga cgcacaagtt gttgagatca ataaactgga tattaccaac
    11161 ttggacccac gactcaagga aaaaggaccg gcttacccgg gcgatttatc aattttacga
    11221 aatagtaatg cgcttatctt gaatgtggat tatccgctgg gactggcagc atataatatc
    11281 ttgtccaaag tagccgaaca cgccagcccc gtactgggca tttacattat gggcaaggct
    11341 gctagcttaa atggtgtggt tggggacgtg atgatcccca acgttgtgca tgatgagcat
    11401 tctcacaaca cttatctgta tcagaatgcg tttacagcgg catcagtatc gccttatctg
    11461 gtctacggca ctgtgctgga taatcaaaaa gcagtcagtg tactgggtac gtttttgcag
    11521 aatgctgaaa ttatggatgt cttttatcgg gaaggataca ccgacattga gatggaggct
    11581 ggaccgtatt tatctgccat ctacgagatg tcacgcccga agcggcaccc ggtgaatgag
    11641 ctggtaaatc tgtatggtgt gccttttgat atcggcgttt tgcattatgt gtcagacacg
    11701 ccgctcagca aaggtcaaaa ccttggggcg ggaaccatgt cctattatgg tatggactca
    11761 acctatgcga catctattgc aattttgcga cgaattttcc aaattgaggc actaaggctg
    11821 agcgccgatt aggtctatga ctacaatttc gggcgggcag agaaagcgcg cccgcggggc
    11881 aaaccaccac ccttccattc caaggccacg cgagacatac agtgtggtat cttggatctg
    11941 gtactgtccc atctcaaacc gcttgccgta acgagaaaaa gtcacaacag cgccataaaa
    12001 agggagccgg atctgcccgc catgggtgtg acccgccaga accaggtcca tcccaatctc
    12061 ggctgcttct ggggtcagat caggtgaatg atacagcaag atagagtagg cggattcgga
    12121 aatatctggc gctaattgca taaactcctg acggtccagt ggataatctc gatttgcgat
    12181 gcccaaaatg cggacggggc tgccgggcca gggcagatcg atccattcgt cattcaagac
    12241 ccggattgag ctgtcagaga ataatgcctg catctggagt gggccatcaa ccgaaccctg
    12301 aactgcatag acgccgtatg gtgcagatag ctgccccaac aactggcgag tatcggtcat
    12361 cacgccatcg tgttcaagga aagataaatt gagataatct cccgtccaga agatgacatc
    12421 gggtgacaga gcggcaacac gggcaacgat ctcgggctca cgacgagtga ggtgttcggt
    12481 atgcaaatca ctgagatgga cgatgcgtaa ggtttctccg ggtttcaggt ctggcgtctc
    12541 gatttgtaca cggctgaccg tgagccagaa aggttcgata taaaatccgt agaatatcac
    12601 gatggtgagc agagcatcca caattattaa tgggaccgtc atcctgcgac tgccaacgcc
    12661 aggacggcga cggttcaacc aggcgccgta taggccccac gccatgaaga ggaacaagcg
    12721 aacaaaaacg aatatcagcc aaggggcaag cggatcacca taggagagac ccaaacgggg
    12781 caaaacagcc agagttgcgt aatctaaaat aatacaaaaa acggtaatac cgaatgcttt
    12841 ccagatattg cgacgcattt cccatccttt aaggaatatc taaggaagtc gtattataat
    12901 gcaaattctg tgtcttatta aaagcccccc gcaccgggct catcttacgt cctgttgttc
    12961 aatcatcatt acattacagc accccacagg aggtgcccat tgagcagatc acccagaatt
    13021 ccggattacg acctgcagaa ggttgtaact cccaatcgtc ttcttggcct ttggcgaatg
    13081 atgaccggtt tccgattaaa gtacctggga gcaactttaa gcctcggggt atcggcatta
    13141 tccaaaacgc tgacctatat gctactgcgg tatttcgtgg atcattatct ggttgcacaa
    13201 accggcagcg tacctttgat ttttatagcc ctgggctttt tagggctggc gaccatcgaa
    13261 ggcagcatga cattcacaag cgggcgactg gcggcacaaa ccgccgaagg gattacccgc
    13321 cgcctgcgca attatttgtt tgaccatatt caacagctca gcttttccta ccatgatcag
    13381 gcgcaaactg gcgacctgat tgagcggtgc acctctgatg ttgacgccat gcggcgattc
    13441 ttcagcgatc aggcgattgg cattgggcgg attatcctgc tattcaccat caactttatt
    13501 gcgctgatca atattaatcc aacgctggcg gttgtctcga ttgtcgtgct gccgctcatt
    13561 gtagctgcct cgttcttctt cttcaagcgc gtctcaaaag cctatgcagc ttaccaggaa
    13621 caggaagcaa cactttctac cacgctgcaa gagaacttga ccggggtgcg ggtggttaag
    13681 gcgtttgccc gccagaagta tgaaatggac aagtttgagc gtgataactg ggagaagttc
    13741 ctgcgcggca agaagctaaa caccatgcac tcgctgtttt ggccggtttc tgatgtgctg
    13801 tgcgggtttc agatgctgct ggggtacttc accggcgcag tgatggcgat taacggcaca
    13861 atctcagtgg gtgattatct ggcgtacaca ggcctgatca cttacatcat cttcccgatg
    13921 cgcaatctcg gacgcattat tgtgcagctc tccactggca tggtgtcgtt tggacgcgtg
    13981 cgcgagatta tcaaagagcg ccgagaacct ctggatgaag gtgattattt gccggagaaa
    14041 gccgtgcgtg gggaactcca attcctaaat attggctttg aatacgaatc gggcagcagc
    14101 gtgctgagcg aaatcaactt tgaagtttta cccggacagg caattgccct gttgggggca
    14161 actggctcgg gcaaaacaac cctggtaaac ctgctgccaa ggttctatga atatactcat
    14221 ggcaagattg agctggatgg ggttccactt caacgctacc ctcggcgata cttgcggcag
    14281 cagattggga ttgtggagca ggagcccttc ctgttctcca ggtccatccg cgagaatatc
    14341 tcttacggcg tgggccgaga tgtggacatg gttgaaattg aagctgcggc ccgggcagcg
    14401 gccattcacg atgtgatttt gagctttccg cagagatatg acacgattgt gggtgaaaag
    14461 ggcgtgacct tgtccggcgg acagaaacaa cgggttgcga ttgcgcgcac tttgctcaaa
    14521 aatccccgca ttctgattct ggatgactca acgtcttcgg tggacacaga aactgaagcg
    14581 gaaatccggg cggcgatgga aaccctgatg aaaaatcgga cgacctttat catcgcacat
    14641 cggattcaga gcattatgaa tgcagacttg atcctggtaa tggatcaggg acgcattatt
    14701 caaagcggtc agcacgaaac gctggtatcg cagccgggca tgtatcgaga tatttacgat
    14761 attcagaccc ggattgaggc tgaacttgag aaggagatcg ccagtgtcag ctaattacat
    14821 tgaagaagaa gagtttcaaa ctcggtttag cggaaaaatc tttgtccgca ttctggggtt
    14881 gattaaacca cattggcctc aggttgttgg gtttttgctt gccattctgg tggtatcggg
    14941 cctggatgcc tggtttacct acttgagcaa acagattgtg gacaacggga ttgtgccacg
    15001 gaatgtcgat tcactactaa agacgctgga gctgtacgga tcgttaatcg tcatccaggc
    15061 aattgcgaca ttctttttca tcttctttgt cagcattctg ggtgaacggg tgcgttatga
    15121 cctgcgccgg atgatgttca atcacctcca gaagctttcg ctgtcttact acagccgcac
    15181 ccccgtaggt tggattattg cccgggtgac ctctgatacg gagcgcgttt cagatatgat
    15241 tacctgggga ctgctcgatt ccgcctgggc agtgatgaac atcaccacgt ctctggtgtt
    15301 catgtttgca atcaactggc gcttaaccct gatcgtgctt gtgattctgc caattctgtt
    15361 atatgttgcc attcaattta gaaaacgaat tttggttcaa tatcggaaag tacgcaagca
    15421 gaattcaaag attgttggcg catacaacga gaatatcacg ggcgtgcggg tgatcaaagc
    15481 gctggggcgg gaacagggca atctggaaga gtttggcgtg gttacaggcg agatgtataa
    15541 agcttcttac cgggcggcct ggttaagcgc gctattcctg ccgtcggtgc agattattac
    15601 ggcattcgcg gtggggtcta ttgtgtacta tggcggcctg caagccaata ttggtggcgt
    15661 tacggttggt ggaattcagg cgtttatctc ctatgtgacc tttatgatgt ggccaattca
    15721 ggacctggct cgagtctttg cagaaatgca gcatgccatt gcgtctgctg agcggatttt
    15781 ctcgctgacc gatgcagtgc cggatgttgc tgatcggcca ggatcggttg accccgggac
    15841 aattcagggc gatatcgagt ttgatcatgt aacctttgct tacgatgacg aaggcaagca
    15901 ggtgttgcgt gacttcagtt tgctggtcaa gcgcggcgaa accattgcgc tggttgggcc
    15961 taccggcggc gggaaaagca caatcgtgaa ccttctgtgc cgattctatg agccgcaagc
    16021 tggtgtgatt cggattggcg gtagagatta caccgagatc tccatgcacg ccattcagtc
    16081 gcggattggg gtggttctgc aaacgccgca cctgttctcg ggcaccatcc gagaaaatat
    16141 ccgttatggg cggctggatg cgaccgacgc cgaagtggaa gaggcagcga ggatcgtagg
    16201 agcgcatgac ttcatccagc gatttgaaca tcactacgat gagccggttg gggaaggcgg
    16261 caacctgctc tcaaccgggc aaaaacaact catcagcctg gctcgggctg ttttggctca
    16321 acccgaaata ttcatcatgg atgaagcaac cagctcagtg gacaccttga ctgaagcctt
    16381 gatccagcgc ggcatggaaa cgttgatgaa aggacgaacc agtttcgtga ttgcccaccg
    16441 cctctccacc atcaaacggg cggatcggat tctggtcatt gaagacggac ggatcgctga
    16501 aatgggcacg cacggcgaac tgctgcgaaa gggcggtaaa tactatcagc tctacaccaa
    16561 gcagttcagg catgagatga atgaagcatt tgatccgctg cgccaaccgg gtaagaaaga
    16621 acaacctgct taataaatcg aaacaagaag aaccgccccg ataatcgggg cggttttgat
    16681 tttattcggc gggataggtg ccaaagaccc gatcccaaat gggagaagta acaccatagc
    16741 gctgctcggg cgtcttgaaa tgatgaagca tgtgatattt gcgcaagaat ttgagatagc
    16801 gggttttcat tgggaagtga tgggtggcat agtgtgtaat atcgtaggcc agatagccag
    16861 tgatgaaggc tgaaaaaacg ggagcgatcc attgcggggc agacagcagc acagcaaaaa
    16921 ccaggtagaa gagaccgtaa aagatcaaag ccagggggat actgacaaca ggcggcatga
    16981 ccagccgggt tttgagctgc ggttgggcgt gatgaacacc gtggaacaga aagaagatgc
    17041 gctgagttgc cggggttttg gccgggtaat gaaacaagaa gcggtggagt atgtattcgg
    17101 aaaaagtcca caagaaaagg ccgccaataa aaccaaccgg gatatgccac cagacgccaa
    17161 caacaggaac gtctaaaatc gaacgaacaa gcgcataaac ggcaactggt aaccagatga
    17221 ctgtaattgt aattgggctc acatgggtga aaaattccat gaaatcggac ttgaacaggc
    17281 gaatcgggac atcactgtgg tcgatcttgc cgtgagacat cgtacctcct gggtttagct
    17341 gacaaaattg attttgaaac tcagattgag atcttacgta tattatactg aggtgaaacg
    17401 aaaatggaag aaaataaagg caaggttccc tggtatttat ggcccttctg ggcgatctgg
    17461 aagctggtga ttggcattat tgcactgacc ggccgcctgg tcggcgccat attgggattg
    17521 gtgttcttga ttgttggcgt tgtcttgacc gtgaccgtta tcggcgcgat tgttggtgtc
    17581 ccatttatta tctttggctt gctgctggtt attcgcagtc tgttctgata tcagcgggat
    17641 acagtccagg attcttgaat gggatcagcg ccagcataac tgctggctcg taaaactgcg
    17701 cccggttgat ggcgttgtcg gtcaagatgc cgacagcgcc attttcctgt ttggaattag
    17761 aacgcgaaaa gacaatatca tctgcctcgc ccaattcttt gccctgccga accagctttg
    17821 atacctgggg tggcagacag aatgttccgg tgcgcgccca gcccatttgc tgggccgaac
    17881 agatgacgac ccaggcaaag ccaatcaaat catccatcaa ggtatcgacg cctccctcaa
    17941 tgcctaccca atagtctgca tcaggatggg cttgccgggc attcagagcg cgctgccgtg
    18001 cgccctgata tgtttctaaa tcgctgaagg gttgagcaga gacacctgaa ggcacagaca
    18061 cgccgataaa atcatagctc cggctgggga acatgcgcgc aaaccctaac tgcgcggatt
    18121 cgatttttac cgggttttta gaggctacta ccactcgggg catgggtcat cccattcatc
    18181 agtaatatag tcttccaggt tttcttcatt aacaacactt tccggcacaa cgcggccgcg
    18241 gacggacatt ccggcgcggt gaaccgattg acggtttcct gaaaccaggg ggtgccagga
    18301 tggcaaatct ttgtcctcat aaatcagccg gtaagcacag gtctttggca tccaggaaag
    18361 ctgagggata agttccgggg ttatttgcag acaggtggga acgagcctgg tgcggcgttt
    18421 atagacgcgg cagcggcaag catgcaggtc aagatagcgg caaacaacat atgtggtagc
    18481 aatttcgccg gtatcttcgt cttcaaggcg gatcaggcag cattgggcac acccatcaca
    18541 aagtgattcc cattcctcgg gagacatctc agacatagat ttggttttcc agaatggctg
    18601 aggagaatct ggtagggtca ttttctatcc tgattacaat gccatttttc cagcagctca
    18661 gcttcgcgtt gggaagatat gccagcccgc caggtatgag gtgggcgcga aagttcccaa
    18721 tccagggtct cgcggactgt ttcagataac ggccgatagg ttaagcctga ctgccgggct
    18781 ttagagatat caaaggcata aaaaccggcc tgggttggat cattttcagg catccacagc
    18841 ggcaacccca tccagggatc aacgccctca gacagtagaa aatcgtctgc tgcccaggtc
    18901 agttggacga gcgattgtgc caattggcaa cacgcatcca tgagttcgcc catggtatgc
    18961 atgccagccg ggctgtcagc attgaaaata ccggatgcgc cattctcgat caggcgcaaa
    19021 atccaggcag acaaatcata tacatcaata aattggattt tccgctgcgg ggttccagga
    19081 accagaacgt ctccaccgct gaacaatcga tgcggccaat aagtaaaccg gtcagaaagg
    19141 tcatagcgac ccacaatcag accggggcga attatcaggc tgttgtttgg gaaaacgcgg
    19201 agaatttcct gctcacacaa agcctttagt ggaccatagg tttgcccgtc gacctgctca
    19261 acagtttcat catccagttt tccgactgaa gcagtttcat caattccaat ctggctggaa
    19321 tcggcgtaaa cagacagtgt ggagatgaag gtatattgat cggtgagact ggttaaggcc
    19381 tgtgctgcca gacgggcgac gcgagggatg taagccgagg tatctatcac ggcatcccaa
    19441 tgacgacccg ccaggagaga taaatcggtt tcacgatccc caatcaggtg aagcgcttcg
    19501 ggaaacagat cggggttgga ttttccacgg tgaaaaagag tgatttggtg accgcttgcc
    19561 agggccaatt cgatcaaagc acgccctaaa aatcttgtgc cgcccaaaat gagtaattcc
    19621 atgctagctt caatgctcct tcagcctgag tataaccgaa aatgtaaaat gacaggctgg
    19681 ttcttgctat aataagcgca ttatgaaaaa acatctctgc ctgtttggcg ttgctttgct
    19741 ggggattttg ataaacagtt gtactccaat aaccattacg ccgctgccga caacctctgc
    19801 ggcagcgcct ggaaatcacg cagacatctg gaggccagct atgaacgata cctggcaaat
    19861 tcagttttct ggtgatctgg ataccacaat tgaggcggat atttacgtgg tggatggatt
    19921 tgaaattacg ccagaagaaa ttgaaagttt gcaccagtat gggcgtaagg tgatttgcta
    19981 cctcaatgcg ggcgcgtggg aagattggcg cccagacgcg cccgactacc ccaccaaagt
    20041 tcttggtgag gcaatggagg gttggccagg tgaacgctgg ctggatattc gccagttgaa
    20101 tttgctgatg ccactgattg agacgcgaat ggatatatgt caatccaaag ggtgtgacgg
    20161 gattgatccc gacaatatcc acggctacac gcaatttacg ggctttgaat tatcttacaa
    20221 cgaccagatc cgctttaatc agtttttagc agatgcagca catcagcgcg ggatgtctat
    20281 cgggcttaaa aacgatctgg aacagattga tgatctggta gaagattttg attgggcgct
    20341 aaacgagcaa tgctttgagt atgacgagtg tgattatctg gcctctttta taaaggccaa
    20401 caaacccgtg ttcaatatcg agtacaattt agaggctgaa ggtttctgtt caagggctga
    20461 ggaagccgga ttttattcgc aaatcaaaaa cctggagttg gacgcctgga ggataacatg
    20521 tcggtaaagc attttatggt agagataacc tatactgtgc ccgcagaagc actgagcgag
    20581 atactgccca agcaccgggc attcctgcaa accggttatg atcagggcgt tttacttatg
    20641 tctggaccgc aagaaccgcg aacaggcgga attgtaattg cgcgggcgga atctgtgagc
    20701 gcgctcaaag actttttcaa aaacgatccc tatcaaatca atcaggtggc aacttatcgg
    20761 tttgtggagt ttgtgccagt gaaacaccaa ccctggttgg ggcagtgggt taatcaggag
    20821 tagattaaag ctaattttgg tgcgccaggc ggttaatttg ctctggcttg cccaagactg
    20881 ccagcatatc ccctgcctgg acggttgaag tgccggccgg gtgaacatct gattcaccgg
    20941 agcgggattg catgacaacg ctcaaatcaa aacgctgctc aagctcgcaa agctgcaggc
    21001 caatcaattt tgattgagcg gagatctcaa tccgcgccag actgttgggc tgcccggcaa
    21061 tcacaatagg tggtgtaatg tcaatttcga cagcagtggc agcaaaaacc ggggcagcca
    21121 ttccggtggc gctgagggcg cgaaaaccaa actgttgccg cagggcttcg gcaaaatcat
    21181 catcaaaaat tcgaaccaca acttgaattt gcggattcag atttcgggct ttaagcgcaa
    21241 tttggaggtt aagactgtca ttctgggtgc agagcaggat ggccttggca cgccgtatcc
    21301 cagcagcctc aaggatcacc tcgcggctgg catcatcttc aataacaggc acatccaggc
    21361 gatggacaga ggccaataaa tcggggttgg gagacagttc gacaacaact acgtcctcat
    21421 ccaactcgcg aagctgctta accacgcggt agcccagatg tcccaaaccg accaacacga
    21481 catgtttact aaaagtagat gcaactgcca tctcccattc ctttccacgc gcccggcggt
    21541 tgaacaacag agacgcgaaa tcggtaacgc caagagctaa aattcccagg cctaaaaacg
    21601 gcaggatgta ataaaagagt tgtaagtacc acgcctgcgg ataacctgcc atgggttgaa
    21661 aaaagatcag gctaaggaca tggtaaattg aagcacccag gcccccaatc gattcgccgg
    21721 tccgctgtgc taatagatag taagcctccc cgccgccaac aaccagcagg aaaaacacaa
    21781 atagagggaa ccaaaactcc ttcagcagca gcaaggtatc gcgccaaccc gcctggattt
    21841 tacgatagac gggcttgcgt tgggttttca ttgatcctga ggatattcgg gaaccgggaa
    21901 gcgcgctgaa agacggcgaa ttggatcgcc agtggacggg aaaacgcaga gggcgacaac
    21961 tgtcatatca tcatttgggc gaccatgatc cagccggact gcttgctgca acagcgaatc
    22021 agcaatttgc tgggctgtag gcgactgctc ttcaatcatg gcaagcaggt gcgtgcaaat
    22081 atccaggctc tcgcctgatg cctcgcctgc atgtgagacg ccgtctgtgt acatgacaac
    22141 ggtcatccag acctgcaggg gaatttctga aattgaaggc cgaacactac gagcaacacc
    22201 aattgcaccg ctttcggtgc tgatgcattc cacgcgttcg ttctgggcga taaaaaccgg
    22261 ggtcgggtta ttccggctga gtaccagggt gccggtttgt aaatctgcag agagaatgtt
    22321 gaggtagaca ccggcgcggc cgctgcgctc tgtgtacagc gtgtccgaag ccgcacgggc
    22381 agcagcgcca tcgcgaacac catcagccag caatccaatt acccggcgga cgaccattgc
    22441 agagaccgat tttgcttcat gccccgaggc cttgccgtca gccatcacaa ctgagatgcc
    22501 cccaccaggg cgttcgacca actcaagcgt atcgccgctt tcttcagagc catgtttatt
    22561 gattttggca accgcaacct gtatttccat gccgttgatt ttacttcaaa ttatgcagag
    22621 gtgttagttt ctaaataaga ttatcatgag gatttggcga ttaatatcaa caggcgatct
    22681 ctgcccgacg ttatccattc gagtctggtg gtaaagccgg cttctgcata tgggcgtcga
    22741 aagacctcca agtccgtgtc tgaaccgggg gactggcggg tgatagcata aatttttcgc
    22801 aaaagttttt ctgacaggcg cccgccaccc acctgagcag aaatcaagat aaccacctgc
    22861 ccgcccggct gcaatacacg ccgaatttcg tggacggttc tggattcgta gatatactct
    22921 gttgggaagg ttgccacaac agcattgcaa gaatacgatt gaatcgggat agatccgcca
    22981 cgggcgcgga tcagatgaac gtttctgctg tttgtgcgcc gcacccttct tcgagcttga
    23041 cgcagcattt gagcgctttc atcaactccg acgcagaagc gcaaatcacc cgccagtgcc
    23101 gacagaagca cccccgtgcc agggcccaac tccaaaatgc attcgccgtt gatgtaggga
    23161 acaatcgcac ggatccagga attccagcgc cctaaagaga caacagcggc gacaatatca
    23221 taagaccagg caagctgatg atagagaagc cggaaaaacc aggtaagaaa tcggtataaa
    23281 acttgagatg ccataatcct ctttagggtg ttaccacaga aaattattgg tctgattata
    23341 tcgtgagaaa tgctggcaga gattttattt taatggtgtt aagaggctgg acgaaaccct
    23401 caaaccgtag taaaattctg tccgttcgca gtgggggctc gtctaacggc aggacagcag
    23461 actctggatc tgtttgtaga ggttcgaatc ctttgccccc agcctaacac ctcccatatg
    23521 ggaggtgtat tttttacttg acgaaacttg caatcccggc taaaatccaa ccctgtcaaa
    23581 gcggctgaat ccagccgaaa aactaccaaa tgagcgaagg taacctccat gatgagaggt
    23641 gagaggcgct cgaaggaaaa agaaaatgga aaaagttgtt cttcacgcta cccggcgctc
    23701 tgtcaccggg aaacaggttg gtgtgctgcg ccgcgaaggc aagctgccgg ccgttttgta
    23761 tggtcacggt tttgagacca cggccatttt gctggacctg cgcgaagcca cccgtactct
    23821 ggctcacctg accggctcca gcctgatcac gattgaactg gatggcaagg gtcacgccgc
    23881 cctggttcgc gaaaagcagc gcgattatat tcgcaatgtg ctgctgcatg ttgacttcca
    23941 ggttgtttcg atgaccgaga aaatccgcgc ccaggtgcct gttgtgatta ctggcgttgc
    24001 gccggcagtc aaggacttca atggggttgt ggttaccggg ttgaacgaga tcgaagtcga
    24061 agccctgcca caagatctgc cccagcaatt cgtcattgat attgccaatc ttgtcaatat
    24121 tggcgatggc atttacgtgc gcgacgtggt tgtttcggac aaggttcata tccacgaagg
    24181 caaggatgaa atgatcgtcg tcatcactgc tgaaatgggc gaggaagctg cggaagaagc
    24241 ggccgaagct ccagcggaac ccgaagtgat tgaacgcgga aagaaagaag aagccgaaga
    24301 gtaagcctga ggctccaatc tacgaactat cgacaaacgg cagcggtgac gctgccgttt
    24361 gttgattaaa aacccagggc tgtcaggaaa ttcagagtga tgcgcgccga aatcccccaa
    24421 aggagttccc cttcgtacag atcgtagtaa atgacatgct catcggtttt gcctttggca
    24481 atgaacggct tttcagtcca atttttgggg tcggccagcc agaacaaggg gatggtgaag
    24541 gcacgggcta cctcaaaggg cgataggtct aagtcgtatg gccagggaat aacaccaacc
    24601 acaggggtaa caaagtaatt ggtaatggtc ttcaatggct gcagacagcc caggatattg
    24661 acatcttccg gcagcaggcc gatttcctcg tttgcttctc gcagggcggt acagatcgaa
    24721 tcgcgatctt cggggtcggc cccgccgccg ggaaaggcga cctgaccttt atgttcacct
    24781 accatgtggg agcgatgcgt gagtaaaaga tgccaggagt gatccattct gagcaatggc
    24841 agcagaacag cggcccggcg aaaaccctca gggacgggca attggtcttc aacgcaggaa
    24901 ggatggagag atgcctgaat gcttttttca tccaagtggt ttatttctac atggtgtttc
    24961 attgagggcg caccaatccg ccgcaaaaat tgcagaccac tgtgctggca tcgatccact
    25021 cgaggtcctt tgcaagcagc ggcgctccgc aattagggca gttcagcggg agctgaatat
    25081 tttcacgaac agggctgaca tctccaggaa gcgtgtccgg ggcactggct gatttctgct
    25141 caatccaggt ttgcagcgct tcggcctggg ccggggcgcc caagtcgcgc agctcggaaa
    25201 tggttatttg ggctgatcgg tgcagctccg gccaacgggc ttgaagtgcc agtgcgttca
    25261 aggcgcgata aatcaatccc aggccggatt ccgggtcgcc catcagaaca cgacaacgcc
    25321 cggcttgaaa atacatcaga ggggaacgta caggggccag acgatctgcc tcgcgagcca
    25381 gtttctcaaa agcaactgag gcgcgatgat agtcgccgtt gttcatccag cggcgagcct
    25441 gttcaagcag tgtttgcggg ccagcgtgag ccgggccccg gcggggcggc ggcaagccgg
    25501 gacgattaat cttccggggt ggcatcatcc acctcggcgc cgctgtctgg agcaggaaca
    25561 gaaaccagac ctgcctcggc cagcaaagtg ccagaatcaa cggaacgatc aataatcacc
    25621 gggcgggcga agattagata acccgtgtgg gctaccatgc gatctgtggg gcgcagtcgc
    25681 gtaggctcgg ccttatagta gcgcagcaag atttcgcaga cttcaatgaa tgcaaaatcg
    25741 ttttggcgca aggcaaccag cagtttctca acctgattaa cggttggaag aatgctgcca
    25801 aagaagccac ctgcttttag agcagaccgg gcctgcgcga tgtaatcgta gggattagga
    25861 acatcgagga agagcgcatc tacgccagtc tcatcgaagc cctcttcaat gttgcgctgt
    25921 ttcagcgtga cgcgctcgcg caggccaagc cggtcaagat tgcgggcggc ggtgttttgc
    25981 atatcctcgc gaacatcgta gctggtgata tgtccggctt tgccaacatt gaaggcaaag
    26041 gcagaggtca gcgcgccaga gcctgtaccg gcttccaaaa tgtgctgacc ttcaccaatc
    26101 cccattgtga ccaggatgaa accgatttct ttagggtaga gaatctgcga actgcgcggt
    26161 aattcgcgca agagatctgc caacgagggc tgcaagagga aaaatggggc gccattatga
    26221 ctgaaaacct gactgcccca gggtttacca atcagggtgt cgtgatacac aatcccgcgg
    26281 tgggtgtgaa aggcggcacc ggcctctaat ttgaggatga agcgtttgtg gcgcaggcca
    26341 accagttctg ccaggtcgcc gtcgtttgcg tgggtaccat gtaaattcca ggtcattggc
    26401 gcggctccag gccaaggtgc tggctgaaga attctgtaat agcgaggtaa cagcgaaccc
    26461 ggttttcaaa cttcaagaca tcgtgccctt cattttcaaa catcagatat tcaacctgct
    26521 tgccctgcga gcgcagatac tccactacgt cgcgggattc tctttcgacg acacgcggat
    26581 catttttgcc ctgaatgacc agcatggggc aggccaattg atgggcatag gtcttggggg
    26641 aacgttccat caagaactcc cggtcttcaa tggggtcgcc aaccgcaata cggaagtagg
    26701 gtttccaggt ttcgggaata cgctccatga aggtaattag atcgtacggg ccaaacatat
    26761 caacagcacc gcgccaaaga tcggggtggc gcatggcctg cgtcaaggtc atatagccgc
    26821 cgtaagagcg gcccacaacg ccagcacgat gaacatctac ccggggatgc tgggctaaaa
    26881 cttcgagggc atggacgtga tccagacgat cgcgcccacc ccaatcgtga tcaacctgct
    26941 tggtgtagct gagaccatag ccagtgctgc cacgggcatt gggaacaaaa accgcaaagc
    27001 cgttaagggt caaatattga atgagaggaa tggagaacca ggcaaaatct gggcgttctt
    27061 gggactgcgg gccaccatgg atgtaataga cgagcgggcg ttttccatca aacccaagag
    27121 caggggcagg caggtagagg cgggcagaca cgcgcgtgcc atcataggaa acgaatgaag
    27181 catcttcacc gggagataga agctcggtgg gaatgccaag cacgcgttcg ttggtatgcc
    27241 gcacgacttg atccccatca atggtgtata tttgagttgg ggaagttgcc gtagaaaacg
    27301 aaagcgccat gcggtctccc gcctcgtcgt aaaggtgaga gaaaagtaca ccattggcta
    27361 attcgccttg accgaccagg acatgagata ccgtcaagct cagttttgat gcatcaaaaa
    27421 gcacttcata gagccatgag catccatcta tgttatattc aagtaaataa cgattatctt
    27481 tgaggtgctc cagcgagacg tgttcaccaa caccagagtg tgcaaggcca atgacttcaa
    27541 tcggtttgag ctcgccgggg tgagacagat ctagataagc ggggctgaag gtatcggtat
    27601 agagcgcggt ggtcaccagg aggccctgtt catcaggcgt aaagttgagg ctgccaatgc
    27661 cattgagcgg gatttcctga ctgggaaggc gatcttcaag cggaacgcca tacagaacct
    27721 tgcgggtgcc gttttcccag aggtaaaccg tcacatcacc aaaagtatag ccttccagca
    27781 gggctatccg gcggtggtct tttgagaaag cagccggata ggtgccccag gggctgactg
    27841 ccaattcagt gatgctgcct gttgccaggt tgcattggta ggaacgaaca accggttcct
    27901 gacgcgattc agcgccaaga taaaccagat tgtgtttgtc atcacccagg gttacatgga
    27961 cacgcgtgcc ttcaaaaaag ccgttgaaag cgggttctgg aaaaccgcca tccagcggga
    28021 tcagcatggg gaggtagttc tcgtcgccgt cgtgatccag cagcaccaat attttaccca
    28081 gggccgggaa gaccaaaaac ggcgctccac caaccaggtg cgggttctgc agggcaatat
    28141 cgggcggcaa gagcggctct ggtacagaag cgccaaattt catccgataa aggctcaagc
    28201 gtccactcag attggagatg aagtaaatct ggtctcctac taactgcggt gaaacgaaca
    28261 agcgcgcgga gagtaatgat tcaacatgaa acatagtcta tctccttctt tctgtttttt
    28321 tctcaacctg aaccaggctg agccaatagc cagttccgag gacagccagg ctgcccaaaa
    28381 tggcagtgcc gaggtgcaaa ggaccaatac tggcaaaggt ccagttcagt gcggcgccta
    28441 ataaatgccc ggcccaaaaa ccgatcaggc tgagcaacaa gtacaaaatc atgcggccta
    28501 atccgccgcc tttccaaaga tggaagacaa aaccaagtag agcggcgatc agggttccaa
    28561 aaatcaccga ttgaattgtc atgcagcatc ctcctcgtcc caggctgaga ttattccgcc
    28621 gcccagtact tcatccccat tatacatgac tgctacttga cccggggtaa tatcacgcag
    28681 cggggtttga aatgccatat gggcgtggtt atcgtgtatg gtgatatggg cacgcatcag
    28741 ggatgattta taacggattt tgacaacggc atcaaattgg gcggctgacg gctcgccaag
    28801 gatccagttg atgggaccag ccaccagatt acgccgcccc aattcgtcag ctcgaccaac
    28861 gatcagttcg ttgcgctcaa tattttttga gattacatat aaaggctcag gggcggcaat
    28921 ccgcaaccct ttgcgctgac cgagggtata aaaagggagc ccctggtgca ggcccaactg
    28981 ctggccctga cggttaagga tggggccggg ttggacggat tccggggccg aggaacgcaa
    29041 aaagtcgcgg taatcggcat cgccaaggaa gcataaatcc tggctgtcgg ggcggtcggc
    29101 cgctgcaagg cctaattgtg cagctctctg ccgcacctcg ggtttggtta agtgccccaa
    29161 agggaagatg gtgtgctgca attgagactg gttcagcagg ctgagtacat aggattgatc
    29221 tttatgtaca tcgagaccac gaagtagatg aatttggccg tttgaatcct tatgcgtgcg
    29281 ggcataatgg ccagttgcca gatagtcggc gcccaggttc tgggcctgtt ccagcatata
    29341 gccccagcga atcgtgcgat tacataacag acagggatta ggggtttgcc cggcagcata
    29401 ctcctggata aagttttgaa ccacgtgctg gtaaaacaat ggtccagcat ccagggcata
    29461 gaagggaatg cccagttttg cagcaacgcg ccgggctaca gccatggcat cgggggtgca
    29521 gcagcgattg tcctcttcct gtccggggat actccagagc cgcagcatta tgccgatcac
    29581 agaatagccc tgctcaagca gcagggcggc tgcaaccgag ctatctacgc caccggatag
    29641 accaacggcc acgcgagggc gatctgacgt catttgtgct ctccgcgacg ggcctgagtg
    29701 actttttgtg gcaaaattgt taagaagttt tctatatctt caagagttgt gtagcgccca
    29761 accgtcaccc gcaaagatcc caaggcccat tcaggaggta tctccagcgc cagcaagatt
    29821 tcggaggggc gcggactgcc cgatttacag gcagacccgg aagaacaggc aaagccagca
    29881 gcatccagca gggctaacag ggtattgcca tctacatctt caaaaacgaa actggcgtga
    29941 ttgggcaggc gagaccgggg atgcccggtc agacgcacat cagggatgct tgccagaaca
    30001 ccggcgatta atcggtcgcg caattgcaga aaatgggctg tatgatcatc gcgttccaac
    30061 tggatcaagc gcaatgcctc ggcaatacca agagcggaag ggatgtcttg tgtgcccgcc
    30121 cgcagaccgt tttcctgctt gccaccggtt tgaacagcct ggatgggcac accacggcgg
    30181 acaaacaata caccgccgct tttagggccg tataatttat gcgcgccaag cgacagcaag
    30241 tctaaattat ctgcctggac atccattggc aaataggcag ccgcctgaac tgcatcggtg
    30301 tggaatggga tgccggcctg acgacaaacc cgcccaattt cagatattgg attgatagaa
    30361 ccgatctcgt tgttggcata aacgacagac accagtgcag tggataagcg gatgcgagag
    30421 gcaaccgaag ctgggtcaac gcggccaaat gcatctacag ggagcagttc aagatcaaag
    30481 ttttcaagcg cggccagctg ctgggcggta tgcaggacag ccgaatgttc aaccggggat
    30541 accagaatgt ggttcgctcc ggtctgcata cggcgcgcac gagcaacacc gcgtaaagcg
    30601 aagttatcgc tctcagtgcc gcaagaggta aagtatattt ctgatggatg agcatttaaa
    30661 aaagcagcta cggcttcacg ggcagattcc aaggctgcct cagcccgctg gccaaaccag
    30721 tgtaccgacg agggattacc atagttggcg gcccaggcat ccattatcgc ccgctggacc
    30781 cgagtatcca ccggtgttgt ggctgcgtaa tccaaatata tggggttggg ttgcgtcata
    30841 aggtctcctg ggctgctccc ccgcagcgtt gtgtaacctt ggattatagc acctatcagt
    30901 gcatcttcaa tcctgatata ataacatcag tttgtcaaat atccattcat tttagggagg
    30961 ccggtatgag tacccccatt cgttttggca ctgatggctg gcgtggtgtc attgctgaag
    31021 attatacgtt tgataatgtg cgccgctgca ctcagggttt tgcagattac atgctgcgcc
    31081 acgggaaaaa ggatcaatgg ttcatcgtcg ggcatgataa gcgttttcat tcagagaatt
    31141 ttgcgctggc cgtggccgaa gtactggccg gcaacggtct gcgagtgtac ctgaccaatg
    31201 gagctacccc aacccctgtg attgcgttct cagtggttaa tcgcaaggcg gccggggcta
    31261 tcaatatcac ggcgtcacat aatccgccga ctgataatgg tttcaaggta cgagacgaga
    31321 acggtggagc aatttcaccg gaaggtttga atgaaattga aagcctgatc cctgattcgg
    31381 tagcaggtgt gcgtcgaatg aaggctgccg aggctgaagc tgccgggcgg ctggtgcgat
    31441 tcgatccggc gccggcctat attgagcatc tggcagactt gattgatttg cagcccatcc
    31501 gcgatgcggg gttgaatatc gtggtggatg cgatgtgggg gaatggggcg ggctggttct
    31561 cgcacctgct tgcgggtggc acgaccaaag ttaccgaaat tcataacacg cgcaatccca
    31621 ttttccctga gatgaaacgc ccagaaccga ttcagccgaa cattaatgtg ggtttgcaaa
    31681 ccacggtaga tctgggagcg gatgtgctgc tcatcaccga tggcgatgct gaccgggttg
    31741 gcgttggaga cgaacagggg cggtttatta accagctcca ggtttatgct ctactggcac
    31801 tgtatatgct ggaagtgcgc ggtgagcgcg gcccaatcgt caagacctta tcaacgacca
    31861 gcatgctgga aaagctgggc aagatttatg atataccgat ttatgaaacc ggcgttggtt
    31921 ttaaatatgt tgcacccatg atgattgaaa cccaggcgat gatgggtggc gaagaatcgg
    31981 gtggctttgc ttttagagat aatgtgccag agcgcgatgg tattctggcg ggtttgttta
    32041 ttctggatat gatggtcaag ctaaagctta aaccatcgga gttaattgac ctgttgtaca
    32101 gcaaggttgg ggcgcattac tacgaccgca ttgacacctc cttcagcgga gaccgcaagg
    32161 cacgcgaaga tatgatcttg agcgccaagc cagaaaccat tggcgggtta aaggtcaccg
    32221 gtctgaacac aatggatggc tataaattct cgctggaaga tggcggatgg gtgctgattc
    32281 gcttctcggg aacagaaccg attatgcgcg tttactgcga gacaacccac aaagaagccg
    32341 ttcaggccat tctaaaagac ggtatgaaga ttgcagggtt ggcctgatct gtgcttaccg
    32401 acacacactg tcacctgaac cttaacttat ttcaagagga tattactgca gtattggaac
    32461 gcgcccaatc ggcgggtgtt gaccggatta tggtgccagg aatagacctg gaaagcagtc
    32521 gagcagcgat caggctggcg gagcagcatg atgcaatctt tgcagcggtg ggtgtgcacc
    32581 ccaatgatgc ggctggctgg gataaagcct ggtttgacga gttggaacag atggcaaaac
    32641 accctcgcgt ggttgccgtt ggagagattg ggttggatta ttatcgcgat cgctgcccac
    32701 gcgccaaaca gctggctgcg tttgaagctc agcttgcatt ggcaaaagca tgtgacctgc
    32761 ccgtggttct gcacagtcgc caatcccttg gcgatatgtg ggcggtctta caaagctggg
    32821 aggttgatcg ccgccaacaa caggcgccat tcgaagcagc ttatggtgtg ttacactcgt
    32881 atgaaggccc cgctgaaact gcctgtgccc tgatcgagac tggattttat atcggcatca
    32941 atgggcctgt gactttcaaa aatgcgcttg accgtcatga cgttgttcgg gttttgccat
    33001 tagaaagatt gcttttggaa acagatgcgc cctatttgac gccccacccg caccgcggac
    33061 agcgcaatga acctgcctat atccccttga ttgcccaaaa aattgccgat ctcaaagcgg
    33121 ttccagtggc aacggttgcc gaaacgacca gcatcaatgc agaccggctg tttagatgga
    33181 gagcttttgt tgcgtaaatc ggcccaattc ttatctctgg ttcaagaaga ccttgctgaa
    33241 gtggaagcat tgctgcgcac gcaggcgcat gggtatcacc ctgatctgca ggcggcgttt
    33301 aatcatttgc tgcagtccgg cgggaaacgc gttcgtccgg cggtcacgat gctgattggt
    33361 catatgctgg gtgcgcctca tgatcagtgt atcacgctgg ctgcatccat tgaaatgctg
    33421 cacactgcca cactggttca cgatgacctg attgacggct cgctgctgcg acgtggctcg
    33481 ccaacgttga atgcgcaatg gtcacctgga gcgactgtat taacgggtga ttttctcttt
    33541 tcgattgcgg cccggctggc cgcagagacg gactcaattg aggttatgaa ggtttttgcg
    33601 cgtacattga cgatcatcgt caacggtgaa atctctcaat tgtttaccag ccgttgccag
    33661 gcaagtcgag aaaattatta tcaacgtatc tacgccaaaa caggatcgct gtttgagaca
    33721 tcagcttatg ccgcagcatt gatcagcccg gtggatggaa aaatttccga agctatgaag
    33781 cagtatgggt acgagattgg cattgctttt cagcttgttg acgatgtttt ggactttacg
    33841 gccgatgaag caacgctggg caaaccagtt ggcagtgatc ttcgtcaggg attggtgacg
    33901 cttccggcga tcctgtatgc cgaatccaac ccgcaagacc cggctgcagc cgatatattg
    33961 aatgggcgtt gcgtacaaga agaagccgag attaaccgat tggtagaagc tatctgtaag
    34021 agcgatgcaa taacgcgcgc aatggcagag gcctcgaact atgtcagccg cgcgatggaa
    34081 tgtttgcaga ctgcgccaaa ctctgccagc aaagctgcac tggaagactt ggcgcagtat
    34141 attgttgccc ggaaccttta aaaacagaaa aagccgcttt gagcggcttt tttgcagtgc
    34201 gctggagagg acttgaacct ccacggatct ctccacatgg ccctcaacca tgcctgtctg
    34261 ccagttccag caccagcgcg tgtgcaagcc gtattataat cggctctgat acattgtcaa
    34321 gcaaaatgag gagcaattat ggcaattgtg ctagatgcaa tgggaagtga tcattatccg
    34381 cagccggaat tacaggcagc cgttgaggcg gcccgcctgt acggtgagga gattttgctg
    34441 gttggcaaag aagccatgct ggcagacggc ctgcgtcaat taaatactgg cggctacccg
    34501 gtgagaatcg tggatgcccc ggacgtcctg gaaatgggcg aaaaacctgt tgacgccgcc
    34561 cgacgcaagc cacggaactc gatggctgtg ggcttggaac tggtcaagaa aggagaggcc
    34621 agcgcattcg tcaccgcggg caataccggc ggggctatgt tcaacgcgta ccgcacacta
    34681 cggtttttag atggcgtgct gcgcccagcg ctgtcggcac ttttcccagt gcgaggcgga
    34741 cgggccgtga tcaccgatat cggcgccaac tctgattgcc gccctgagtt tcttttacag
    34801 tttgcggttc tgggcagcgt ttatgccagg cggattttga aaatcgaaaa tccgcgcatt
    34861 gggctgcttt ccaatgggga agaagcaggc aagggcaacc agcttgtgcg agatacttac
    34921 cccttgctgg agcagagcgg cctgaatttt gtgggcaacc tggaaggcaa agaggtttat
    34981 ggcgggatgg ccgacgtggt catcaccgac ggatttacgg gcaatgtgtt tctgaaaagc
    35041 agcgaagccg tggcaaagtt cttgctggag atgatgaaag agcagttgac ggcctctttg
    35101 cccgcaaaac tggctggggc aatggccaag ccgtatctca tgaatttgaa aaaagtcatg
    35161 gatccggctg aagtgggagc ggcgcctctg ctgggtgtga atggattggt atttgtgggg
    35221 cacgggcgtt ctgatgcccg ggcgctggtc agcgccattc gggcggctcg cgaagccgtt
    35281 caggctgatt tgctgggcgc gatgcgcaca gccattcaag aacaactggc gcagattgat
    35341 gcgctgatca ctcaaaacgc ctccgggcag aaggaataac tacatgcgca tcgttatgga
    35401 cgaaaaaatg gtaaagcgca atcaaaaggt tgcgcagtat acctccctgg cttcgctggt
    35461 catcctggtt atcgggttga ttgtatcgtt taccaaccct gagcaaatca tcatctcttt
    35521 tggggcactg ctgctgggct ttttactctc tcaggtgggt attttttacg gcaaccgctg
    35581 gggcaaatca ccgcgcgccg atgaaattct gaatgacgaa ctcaaaggcc tggatgagcg
    35641 ctatattatt taccactact gtacgccagt tccgcacctg ctggttggtc cggcgggggt
    35701 ctgggtgctg gcgccatatt ctcaggccgg gaaaattgag tatgacgcgg ctaaagaccg
    35761 ctggtcgcac cgaggcggca actggcatct caaggtgttt gggcaagagg gcctgggacg
    35821 ccccgaccga gatgccaaga taacgctgca agatgttaat aagtacctgc acgacctggc
    35881 aggggacgac ccgcggcggc ccgaggcgca gcttgcgctt atcttcacca atgccaaggc
    35941 tgaggttcaa agccaggaaa ccccactggt ggctatgccg gtggcaaaat taaaggactt
    36001 tattcggcgc aaggctaaag aagtcaattt cccggcagat acgcagacct ggctggaaga
    36061 agaattgccg taaaacagag cactggttga taaaaaatac cgggccttga caagaaggtc
    36121 cggtatttta tttcaggcta cttttcgggg tgggtatttt ttgtgggtgg gttcgggatt
    36181 ggctgtggca cttaagcggc ggatttcata ggcggtgaga tagcgccatt cgccgggttt
    36241 aagatcaccg aggagcaagg tgccaattcg gatgcgcaca atccgcagaa cggggagtcc
    36301 gatgcggata cccatctcgc ggatctggcg tttacggcct tctcgcaggg tgatgcgcag
    36361 ccaaatgttt ttcccggcgg tattttcgat gcgcacatcg gcaggggcgg tgcggtgtcc
    36421 gtcttcaaga acaaccccgt gacgcaaggc agccaactgg cggtcatccg gacgttcctc
    36481 taccagcacc cgatattctt tttcgtgacc ataacgcgga tgggtcaggc ggttcgccag
    36541 ttcaccatcg ttggtcaaca aaatcaagcc ttcgctgtcc aagtccaagc gacccacggt
    36601 aaagaggtgt ccaggcacag gtatcaggtc acgcaccgtt ttgcgttcat cctcggcgcc
    36661 cgaatctgag agcacaccac ggggtttgtg cagcatgacg tacaccatct gcacttcggt
    36721 cttaggtaat ttgcgaccat ccacaataat ttcatcgttt tgcagatcgg ctttagcgcc
    36781 cagggtggca attttgccat taacgctaac gcggccggcg ataatgaggg tttcagaggc
    36841 gcggcgagag ccatagccaa aacgagccaa gattttttgc agtcgttctt ccattgagat
    36901 cagtccttta agataacatt acgctcttca tcaggtgttt cggggagttc gtagggcggc
    36961 atctgaccta acgatgacaa tccgaaatgc tgcaagaact cagtggtggt gccgtagagg
    37021 attgggcggc ctgggccttc ggcgcggccc atctcttgaa ccaggccttt acttaacagg
    37081 cttttcaaaa cgccatcgct gttgacgccg cgaatggcat caatgccggg gcgagtgacg
    37141 ggctggcgat aagcaatgat tgccagagtt tccaacgctg cccggctgag gcgtgcagtt
    37201 gcctccaggc ccagaaaatt ctcaacaatc gaggccagca tgggggccgt ggtgatctgg
    37261 attttgccct cgtgacgctg aaggctgatg ccacgcccct gggctagctg aacttccagg
    37321 ttcgcgatgg cttgttcaac cgcttctaat ggcctgccga gcgcctgggc aagctgagca
    37381 accggaaccg ggctggcggc gacgaataga atcgcctcca ggcaggcctc gagagataat
    37441 ggcaatgtcg attcgggtga tgcgtcagtc atgctataat atacaatctt gaaggtcaaa
    37501 tttttaatag agttcaatta tacctgctgt atgatgaatt gtcacccaca acccaaaaac
    37561 gctaaaatta ccggctggtt attactttta ttattactgg ctgcctgtac gccgctagcc
    37621 cttaccaatt cggggatggt tgcggtttct attcaggtgg atggggaggt cattgaggct
    37681 aacacaccgg cgggtaccac tgttgctgac acgttagaga acaacgacat catcctggac
    37741 acattagacc gggtggagcc cccaagctat accattattg ctgaaccgac aaccattacc
    37801 gtggtaagaa ttcgtgaaga atacgagatt gaacaggcta ttgtaccgtt tgaacagcaa
    37861 actgttccaa atgagtcatt acctgacagg cgcacgatgc tgattcaact tggcgaaaat
    37921 gggctgcaag agattactta tcgacgagtg tttgaaaatg gcattgagac ctcgcgaact
    37981 gtattcaagg tggtaacgct caatgaggcc agaccagaaa ttgtgatgat tggggtacag
    38041 tcccccttta caccaatcag tatccccggt gtgattgctt acctgacagc cggcaatgcc
    38101 tgggtgatga gcagcacaac cggtgaacgc cgtccaatcg ttacaactgg ggacctggat
    38161 ggccgggtat ttgcattgtc gccgacaggg gaatggctgc tctatacacg gaaaagcgag
    38221 ggcgaagaaa aaagcatcaa caccttatgg gtagtgcgga ttacagacga cgagcccgag
    38281 cctttcagcc tggatgccga gaatatcatt cattttgcgg cctggatacc atcggatatg
    38341 ctgatggtgc tttactccac cgttgagcca agggctacag cacctggctg gcaggccaat
    38401 aacgatttac gccggattcg gttctcggaa aacgcctata ttcgcgacga agaaattatg
    38461 gaagccaact ccgggggaat ttacggttgg tgggggatga attttgcgat atcgccggac
    38521 ggcacgcagg tagcctactc acggcctgac agtgtggggg tggttgatct tgaaagtgga
    38581 gcaacaagca ccctgcttga cctactgcca ctgcaaacag gcggtgactg ggcatgggct
    38641 ccgggcatca gctgggcacc ggatcaacaa attttgtatt cgtccgccca catcccaatg
    38701 tcagggctgg caaacccaga agcatcgcct ctgtttaact tgagcgcgat cgtactggaa
    38761 aacggcgcag cgttcccagt agtatcgcaa acgggcatgt ttgcctatcc gacagcatcg
    38821 cctgatacgc tgccggggga tcgttatctg gtagcttatc tgaaggcgat tctaccagag
    38881 cagagcgata ccagccggta tcaattgatg ttgatggatc gcgatgggtc caatcaaaaa
    38941 ttgatctatc cacctgaggg gatgtcggga ttggagccgc aacaggtggt ctgggcgcca
    39001 gcgacaggtg atgagccgct ctggctggca tttatctatc agggtaattt atatctctac
    39061 gagcacggca cggatataat ccggcaggta accggagatg gtcttattga tcgaattgat
    39121 tggaaataat tctgaaggat tgagggctta atatgattgt attgattact ggggccgcgg
    39181 gttttttggg ttcgcatttg tgcgattatt tgattgagca gggacatacc gtcatcggga
    39241 tggataattt catcactggc agtccggaca atctggcgca ccttgccggg cacccccgat
    39301 tcagtttcat ccggcacgac gtagcgaatt ttatctttgt gccgcaaaaa gtagacgctg
    39361 tattgcattt tgcatctccg gcaagcccca acccggcctc gccttatggc tatacgaatc
    39421 ttcccatcca aaccatgaaa gccggagcgc tgggaacgca taatacgttg ggagtcgcca
    39481 aagcacacgg tgcgcgctat ttactggcat caaccagtga aatttacggc gacccgctgg
    39541 aacatcccca aaaagaaact tactggggac atgcggaccc cattggggtt cgatctgtgt
    39601 atgatgaagc caagcggttt gccgaagcac tcaccatggc ttaccatcgg tatcacggtt
    39661 tagacacccg gattgttcgc atcttcaaca cttatgggcc gcggatgcgg ctggatgatg
    39721 ggcgcgtggt acctaacttt atacaacaag cgttacaaaa agaagcattg acagtttatg
    39781 gcgacgggga gcaaactcgc agcttttgtt atgtcagcga tttggtggcg ggaatttaca
    39841 agttgctttt atccagcgaa gacatgccag tcaatatcgg taacccggct gaaatcagca
    39901 ttctggagtt tgcgcgaact attaaccgct tgacgaacaa cccgacatcg atcaagttca
    39961 ttcctgaaca gcgtctgggg gatgatcccc agagaaggcg ccccgatatt acccgggcgc
    40021 agcaaattct cggctggcag cccaccatct cgctggaaga aggcattaca cgtacaatcc
    40081 catactttgt tcaaaaaatg gggctggcat gatcctcacc aacgagcgtg aacgaacccg
    40141 gtttttgcgt tttgctgttg ttggcaccat tggcgcagtg attgattttg gtatcttcaa
    40201 cctgcttatc tggctgggta tgcgggcgat tgtggccagt gcaatttcgt ttatagctgc
    40261 agtgatcagc aattttctct ggaaccggta ctggacctac ccggattcgc gcagcaaacc
    40321 acttgcgcgc cagatggtgc agtttttatt ggtcagcctg gttgggttag gcatccgagc
    40381 tggactgttt gccattcttg aaatcccatt aatcagcctg tttgaaaaac tggcgttgcc
    40441 cttgccgctc agcgcggcgt ttctggggca caatttaacg ctggcaatcg ccattttgat
    40501 tgtgttgtta tggaatttcc ttgcgaaccg atattggacg tataatgatg tcagcaagtg
    40561 actctatgga cgccaattta cctgtgccac aacgcgctga atcccccaaa gacagccaga
    40621 cggtttcttc cgtcaggtca ttaaaggagt tggtggatct agtctcaggc aggaatttca
    40681 acaccaacgc acctcaggaa gacctgggac tggctgaacc cctaccctac ccatttctgg
    40741 cgctacttgg gcaggaagat atgaagctgg cgctgctgct ggcaatcatc aatccggcaa
    40801 tgggcggggt tgcgctgatt gggccacgcg gcacgggcaa gacaacggcg gttcgcagcc
    40861 tgctcgattt gctgccagac gctgagcgca gcgcctgctt ttatggctgc ctgcctgaag
    40921 atattgaggc gggtggcatt gatgcggtct gccccaactg tgcgcaaaag tacgcccggg
    40981 gagaaaaatt gacccgcact gaacgggtac ggctggttga actccccttg aacgcccggc
    41041 tagaagatgt gattggcggg gtcgatgagc gagcggcgct gaatgatcga atgcggttaa
    41101 agcgcgggat cctttcgcag gcagaccgga acattctatt tattgatgag attaatctgc
    41161 tctccgatga aatcatcaat gcgattttag atgctgccgc cctggggacc tataccgttc
    41221 ggcgaggccc cattacagcc acctatcggt cgaggtttat tttagtcggg tcgatgaatc
    41281 cagaagaagg cgccttgcgc ccgcaaatta tggatcgatt tggcctgcgg gtgattgtaa
    41341 cgggcttaaa taagcctgaa gagcgcctgg aggcgtatct gcgcgcacag gctcaccgcc
    41401 ggaatccgcg cgcttcaata gcccagttta tggccgcaac actgcaagca cggatggaaa
    41461 ttgaggcagc gcgatcactg cttcccaaag tgaccatccc ggaacagatt gccagggcgg
    41521 gcattacgct gattcagaag ctgcagattg cttcgctgcg ggcagagatc acactttttg
    41581 aggcggcaaa agcatatgct gctgcggata accgggagac ggtgacactg tccgatctca
    41641 aggttgtggc tccactggcg cttcggttaa ggcgatcaga ttatatgcag cagtatcaag
    41701 ccaagcagcg agaagaagac agcgagatga tccgtatcat ggaagacacg ctaaaaggcg
    41761 aggggtagtc actttggcga aatcaggcac gcagactcat cggcagcgac tggagacgct
    41821 tttaggaggc gggcgacctg atcggacacc cgttgcgctc tggcggcatt ttccggtaga
    41881 tgatcaggcg cccgagcgtt tagccgcagc catcatagcg ttccagaaga gctacgactt
    41941 tgatttaatc aaagtgacgc cggcttcctc attctgtgta aaagattggg gtgctgcgga
    42001 tatctggcaa ggcagcgccg agggcacacg ggcctacggg ccaccggtca ttcaaaagcc
    42061 agaagactgg gaaaatctgc ggcggttatc accccatgaa ggtgctttaa aagcgcagct
    42121 agaatgcttg aagctggtta tgggggcgtt taaaccgcat acgcccgtat tgcaaaccat
    42181 cttcagcccg ttggcgcagg caaagaatct ggcgggaaaa gaacagttga ttgtacatct
    42241 gcggcaatgc ccggaggctg tgcacgcagg actgcggatc atcactcaaa ccacgattga
    42301 ctttatttcc gaagccctga aaaccggcat ggacggcatt ttttatgctg ttcaacatgc
    42361 acaatacggg ctgctgaccg aagcggaatt tatgaccttt gggcgagcgt atgacttacc
    42421 cgtactggca actgcgcaat ccctgtggct caatatgggg catattcacg gggaacagat
    42481 tatgttccag cagatcacag attaccctgt gcaaattctc aactggcatg atcagcatac
    42541 ctggccttca ctcaaagaag ctctatcctt atatccggga ttggtatgcg gtggactgcg
    42601 gcagtgggaa accctggctt atgggacgcc agaacaaatc tgggcagagg cgcgtgccgc
    42661 gcaagcgcag acccagggga aacgcttcat cctcggcacg ggttgcgtta cgccaattat
    42721 tgccccacac ggcaacctga tggcggttcg taacagcgtg gaggcggcat aataacatgt
    42781 ctacgccaag ggtcattggt ggaaccgcac gcggcatcag gctgaaagat gtgccagggg
    42841 atattacacg cccgattaca gaccgggtca aagaggcgct gttcaatatt ctgggcggcg
    42901 atattattga cgcgagcctg ttggatttgt ttggcgggac cggggcggtt ggaattgagg
    42961 cgttatcacg cggggcggct tttgtacgga ttaatgactt gaaccgggct gcgataacga
    43021 ccattcgagc gaacctggaa gcgaccagat tgcagagcca tgcccaggtt acccaaggag
    43081 acgcgttcag cctgcttcaa caggcggcag atagggcgtt tgattacatc tacattgctc
    43141 caccccaata taaggggatg tggcagcgag caattaagct ggtggatgat catccaaact
    43201 ggctgtcgga tgaggcctgg gtcatcgttc aaattgaccc ggttgaatat gaggcaatga
    43261 gcctcaagaa tctgaatgag tttgaccaac ggcgatacgg cagcacgctg ctggtgtttt
    43321 atgagcgtgg aaatgacgaa gacgacgcct caaacgccga actcgatctt tagttttcca
    43381 tccgtataaa aagaccgccc aaggaaaggc tgagtgctgc gcattgatgc cagtacacgc
    43441 ggtagaataa ccggcaggtc ttctaccagc ggcaggtttg agagggaagc ctgcgatacc
    43501 caggccaatt cgccttcaga gccggggaca agctcaatag tggttttatc tacttcgccc
    43561 cggaaaatga agatgccaat gccgcgatcg ggttcaacat caaccataag aatgccgcag
    43621 agccataaat ctgcatcatg gacgccggct tcttcattta attctcgcct tgcagcgaaa
    43681 aataaatctt ctccaggttc aacatgcccg ccaataccgt tatagcgatt agcccagagg
    43741 tgtttggttg gagcgccttt gagcagcaaa atatcgctgc cgctggtcag gaagattaat
    43801 gtgcggggaa taattgcata acgattgggc gtaacaccct gatcttgttt gggcatgaaa
    43861 cctcatctct tgtcattgtc tcttctgaag aaggcagaga tttgctctga taaacaccgg
    43921 ggcggacatt gaatgtccgc cccaggcaaa gtgatttgac tgattgagtt agatacccag
    43981 cagttgtttg acagtgggga tttggccggc gctgccaagt ttgtggttct tgtccgcaat
    44041 gaaattggcg tattcagaca tgaaaatttt accgatgggg cgcaggtcaa tcgcatcggg
    44101 atggtcacgg aagaattcgc ggtgaacgcg cgtccaaacc agacgaccat cagtatcgat
    44161 attgatcttg gtgacgccaa gttgagcagc gcgcaagaac tggcgatcat ctaccccctt
    44221 ggcgcccttg aggatgccgc ccgcggcatt gatgcgctca acttcttcct gaggaaccga
    44281 actggagcca tgcattacaa gggggaagtt gggcaactgc tgctggattt gttccaggac
    44341 atcaaaacgg agtgcctgat tgcccgagaa tttgaaagcg ccgtggctgg tgccaatagc
    44401 aacggcaagg gaatcgcagc cggtgcgttc tacaaattcg cgcgcagttt gcgggtcggt
    44461 gagcttggca ttggcctcgt caaccgcgac atgctcttcc accccaccca actgacccag
    44521 ctcagcttca actacaatac cgcgagcatg ggctgcatcg accacgcgct tggtaatggc
    44581 aatattagtg tcaaaatctt cgtggctggc atcaatcatc acggagctgt aaaatccgct
    44641 ctcaatgcaa tcgtagcaag cggcctcatc accatgatcc agatggacgg caaaaatggc
    44701 ttccgggaag atgtcgttgg cggttcggat catggcttcc agcatgcgtt tgtcggtata
    44761 ggagcgggcg cctttgctga tctgaataat gaagggggcc tggctgtcga cgttgccgcg
    44821 gaacaatccc atggtttgtt ctatgttatt gatgttgtaa gcgccgatgg cataacggcc
    44881 atatgcggcc tcaaatagtt ttttggttgt tacaatcatt tctgtctcct tttggctagc
    44941 ttaagaacat gggcacgccc ataaagtcac gaactttggg gcggtaggtt tcgatatcat
    45001 aatagctggg gacgtccaca cgcttgcgcc cggcaagaac catttcaacc gggacagtgg
    45061 tgtacttgcc gccatgcaag ccaaccatct taccggtttc attccggcga atgagttgga
    45121 tggcaagatt gccaaaggac atggcaacca tgcggtccag cgagtcgggt gcgccagagc
    45181 gcatcatata gcctaactgc tggtacatga tattttgccc ggtgaggcgt ttgacttcct
    45241 cagacaaaac aactccaaca ccgcccagtt tgcggtgacc ataggcatca gcctcgcccg
    45301 attcaatcac ttcgccacct tccaggatgg cgccttcaga gatggtgacc atggcatagt
    45361 tggatggctt gccgcgtttg tcgtcgatca ggtagcgcgc cagacggcct acatcgcagg
    45421 ggacttcggg gataacggcc cggtccacat cagccaggta ggcactgatg aggctggtct
    45481 cgcctgagtt acggccaaac agttccacga ttccgatgcg ctcatgtgaa cccacagatg
    45541 tgcgcatgtt ggtgatgata tcaacactgc gggtaactgc agtcgagaag ccgatgcagt
    45601 agtcagtgcc aaagacgtca ttatccatgg ttttggggat ggccacaacc ggaaagcctt
    45661 ctttgtggag gcgaactgca aagctcaagg tatcatcacc gccgatgggg gttaagacat
    45721 caatgccaag attggcaagg acattcaaga catggctggt gtaatcaata tttgaggagc
    45781 cctcagcggg ttttccccag gttgaatttt taagaaaatc ggggacggct ttggggtcaa
    45841 cgcgctgggg gttggtgcga gacgtatgaa gaaatgtgcc gccagtgcga tcaattgtgc
    45901 ggacgtcttc gcgtttgagc tctcgaatgt agtagtcgtg agtagagggt tcgtttagat
    45961 cataatgcaa taaaccaccc cagccgcggc gaatgccaat cacccgatag cccagttcaa
    46021 gggcgcccat gacaacagct ttgatgcagg gattcaaacc cggcacatcg ccgccccccg
    46081 ttaaaattcc aagtgtttta gtcgtcataa aatctcagcc tccaaataaa gaataaagcg
    46141 tgctacatgg cccgcaattc ctgctcaggc tcgggcgcgt tcggttcaac cggaatttgg
    46201 aaggttatgc gactgccctg cccaacattc gaatcaattt ccatatatcc cccaaccatt
    46261 tcaacacgtt cacgaattag tttgatgccc acaccactct cagattcgtc ctccgggcgc
    46321 atgcctttgc cgttatcact gacagagaca cgcaccaggc ggtcttcgat gaccatttgg
    46381 gcattgatct gcaatttata gggtagttct tgattgtggc gaacagcgcc accgattagc
    46441 tcctgcatgg agcggaaaat catcacttca atgtaaggtt caaagcgacg ctcgttgcct
    46501 tttacattca ggctgatgtc ggcaccagac tgttctttga gagtgtcggc gtagcggcgg
    46561 atggtgggaa ccacacccaa atcgtccagc atcatggggc gtagctcgga gatgaagaag
    46621 cgcaccttct ggaaggtgga catggctgtt gttttgaggg cgttaagttc ctctttggag
    46681 cggttcaggt ccacttcaaa gaaacgagcg acaatgtctg cctggacgat gaagttggat
    46741 aaggcttgag ccgggccatc gtgcatctgg cgggaaagcc gctggcgttc ttgctcctgg
    46801 gcattgatca ccagttcaag aacggcagag ccaccgccct tggcgcgcat tgagcgcggc
    46861 ggctggtgac cttcggagag gaatttgtgg gcccgctcca gcgaggcgac ataacgtttg
    46921 agggtttcct ggtcattttg gagcttctca atctggccgc gcatcaccag gaggcgctgc
    46981 tgggcttcga gagcggcatt gtatgcattt tgaatgtcgg ccttcgaagt agcttccacc
    47041 tgggaacgaa cttgctgaag gtgcgcggtg attgcggcgt tacgctgtgt caactttgcc
    47101 agttcggcct gactctgctc gtacatcaat gtgacttcct gtaagttgcg gcgagcttcg
    47161 tctaaatcgg tctgaaccga ttgctgaaat tcttcccagg ggcttgcgcc atcggtattt
    47221 gcgccaaagt tatgcacggt catctcgcgc tccttctgct taaattagtc caaatttaag
    47281 gggaactccc tcaaagtaat aagtatagca caacctgttc aatcaacgca aactgtttcg
    47341 tttgcggccg tcgctgcgga gatgcttgat ctccgcagcg acaaggcgaa cagggttgga
    47401 ttatttggct aacaacaagc gaatctgggc aaagtcgtca gcggttaact cgccggcact
    47461 ggtcgtgctg gggggataat tgctcagcat ttctgcctga gtgatatctg actcagacgg
    47521 ccagatcata tcgatgatac gggtgtggtt ggtatcggca ggggcaccgc caaaacgcca
    47581 ctgggcaacg gcagcctcaa catcacgcac acgccagacg cccgaggcag ggtagccttc
    47641 ctgccccagg accaccgcag tgtaccccca ggttgccgga tcgccttcgc caaacacgtc
    47701 cttgggcacg cgcaaagtca cagttctggc accggcatca gcaataattt tgaattgtgc
    47761 ttcggtaatc tggatgggct ccagcgtttc cgggttgggg gccaggatct ggggagtcca
    47821 gccttcggcc caaacggcat aatcccagcc gctgcccgct tcaagggcgg cattgcgtcc
    47881 aggcagcagc aggcgggcgc cggtgccgcc accggggtct ttgtcaatat agacatccag
    47941 ggtttgaacc gcaaggttgt tgggggagcc ccagggattt ggaatagcgc catagaatga
    48001 gaatttgaag atgatgttgt tctcgtcata ggctacccgg aaactcttga ggtcaaacat
    48061 cttgccaaca aagatggcgt cggtaggata agtgtaagtg cccgggccgt tatcatcgcc
    48121 ttcggggtct tcgacctcaa atactacggt ggcattgccg atttcgggca caattatctg
    48181 agcaggacca ccggtgggaa tgaattgatt gtcgggctgg gcaacggtca gcaaacggat
    48241 gtcatcgcca gtttccatct cgccaaaaag tttgaaggga atggctactt ctatcacgga
    48301 atcaccaact gaggcctcac ctaaagcctc cattttggcc caaccggttt caccacctgt
    48361 ataggcagac aggctgcctt tggcggttac ttcgaccagg tgggtggcag caacacccag
    48421 cagcaatggc tcatcagctg agatgttgac aaatgggttg gaacctgcag cacggggtgc
    48481 gacaaagtag aaacccacct gatcggaagc agacattggg cgggtcaagt caagccggag
    48541 ataaagattg ttggcgtcgt atgtgtagcc cagaccacta gcaaaaggcg tttgcacttc
    48601 agcgggatag gctgcgccca gagaccattc atcaccatca atcttgccat caatgaccgg
    48661 tgtgctcatt ccttgaaccg gttgagctgc ctgcaccggg cggggttgaa tgataggaac
    48721 gttcacaaaa ttggggaccg gttcacccaa tgagtcgaat acgcctttca aaagcgcccg
    48781 gaaacctgtg tcaaagtagt catcctggcc cgaatcctgg tcagcaccgt accaccagaa
    48841 ccagtcagaa ccttcggcca ggtacataaa atcttgagcc tgggcgattg cttctggaga
    48901 agctgtgcgc actttcgaaa tatcgtattt agccagcacg gcgcgcgtct gccgcaggta
    48961 atcccaggcc atattttctt cagcttcacc gagccaggta tcataattgg ggctaaacca
    49021 ggccgcggca aagagattat ccagcgagcg ttgttcgggg aaccattcaa ggtattggga
    49081 tggggtgacg gtttgaagtg tcttgctctc tgaaagcagg ctatagaggc tgtggaaaaa
    49141 ttccttgccg tcgttatcat aattttccca ggcgttttca ccatccagga tgatgctgac
    49201 aatatgcggg ccttcggcgc cttcgtcttt cagctcagta cgaatatttt ctaggcgctg
    49261 gataagatct tcggctgcgg cttcaccgga aataccggaa taagtaaagc cgactttgtc
    49321 tgagatgaca ccatcgcgga aaaagacggc caattgattg ccgtttgcgt ccgtcacata
    49381 gtaggggcga tagagatcat ctgcctgctg gacggtctca gcagcatccc gcgtaaagcc
    49441 atccaggttg aggctcttcg ccaaaacggg ttcaccggtt gccatccagg tgtagccgct
    49501 tttgatgaca aagggcacaa tggtttgcgc cacagcgccc tcgccgggcc acaggccttg
    49561 cggcggctgg ccaaaatttt cctcataaat ggcaacgctt cgctccagat gagtcagggc
    49621 gtcttgcggg tatgagaact ggggcggggt ttctacaccg gggttgccct cgcgcgccag
    49681 attggtatct accaacagcg ggagaatggg atgggcaaac ggtgtggtta tgacttcaat
    49741 ctgcccatca gattgcattt ctttatggag gggaatcact gcctggataa tacgtaaggc
    49801 ttcagcaaaa aggatttgtt tatcggcctc agtaaaattg cggcccttct cgaccaagga
    49861 tttcagggga tcagcagcca gtaggtcagg atcaaaccag gccaggttga accaaatttg
    49921 aagatcgcga taatcctgct cggtgaaggt tgcaagcgcg gattctatag cggccgtgtc
    49981 tgttccgccg cgcagatcaa gcagctcctg gtagcgggga tatacggcaa ttatgtgatc
    50041 ccaattggca tcaaaaaaac gctgaaggat gaaacgcttc tcatcgtctg tcaaatcact
    50101 ggcgggtttt tcggcaagct gccagtagag atctttggcg ccattatcag cgtaatcatc
    50161 cagttggcgg atgagaacag gggtaaggtt gaatgtcaca tggacgtcag gatattcctg
    50221 caaaatggca gccatgtcgt aataatcttt tgtggcgtgg acgcgcaccc aggggcgagt
    50281 atatacgccg gcatcatctt tgtaatacag aggttgatgc tgatgccaga ccaaattgac
    50341 gtacagaaca tctttactgc ctggatgctg ctgaccacag gcagccagca acatcgcgag
    50401 aatgaccaaa aagttaagca caatccattt tgttttcatg ctcattcctc ctcagtttga
    50461 agcgtgtata tttgtaaccc gcccgcggcg gtgcctatcc ataatcgtcc gatgctgtct
    50521 tcagccaggg caagcggctc tgaaccggca aaaccagagt tatgttcatt atagcgtttc
    50581 cagttctgac catcgaagcg aaccaaaagg ccgcccggct cggtgggcaa gccaaccccc
    50641 acccagtaga tgcccgggtc ggtttctgtt atggcttgaa catagttata aggaatgtct
    50701 gagttgctgg tgcggtaaaa cgtccagccg gtgccatccc agcgagccag gccgctgccc
    50761 agcgtaccgg cccacatctg gcctttggaa tccagatata gatctgctat gccagcccag
    50821 ccaagtccca gatcatgaaa atcgtcagac tgccaggatt gggtttgcca gtcaaggcgg
    50881 ctgatgccca aaccgccgcc gaaccagatg acatcgccgt tgggggtggg ttgaatttca
    50941 agcgagtaga tggaagtatc caacaggcct ccgggggcag cagtgtatgc agtccaagtt
    51001 tggccatccc aaacagaggc gcctgacagg gtgccaaccc aaatttggcc cagcgaatct
    51061 tcgtccaggg cattgatttg ggcattttct aatcggatgt cagatgcggt aaagctagtc
    51121 caggtttgac cgtcaaacga tgaaagacct tgctcggttc caaaccacca ggttccgcgg
    51181 ctgtcttgta agagggataa aacgttccga tccatgatgc ccgaattggc aggcacatac
    51241 cactgacctg cctgtcccca gggggcatcc tcgttagggg tccaaatggc ggcgccgttg
    51301 gtcgtcgcca gggcaacccg gccatcgggg gtggttttca aatcatggat ttcattggag
    51361 ggcaatggtg tgcccgtgct gttcatttgc acccagccgc ggcgcaaaac cgccatttgc
    51421 acttcataaa cgactttgcc ggtcaataag acaatgacaa ggccgaaaag caggatccaa
    51481 aacaatttgg gtgggcgggg cagcatctca gagagataat cccaatagga atctttttta
    51541 cgcgcccaat caataaacca gcgaacagcg cccgaggcga tcaggctcat ggctggcgcc
    51601 agaataactg tatattgagg ccacttggtc ggccacacca ggagaattgc caagatggaa
    51661 gccagccaga ccgcggtcca gggacgttgt ttcagcgcca ggccaaaacc agcaatggag
    51721 agccagaaaa taatatcatc tgaagtcaaa aagaagaaaa cattggggtg ccagggtact
    51781 gtggtaataa tccaaattac gggctgatac cagggataat ttgcccgaag gacatctgca
    51841 ctctgggtgt agccggcgtg gaagaaaagc gactgttgaa gccggctcag tggatcgtgc
    51901 cagagggatg gattaaaagc ccaaaacgag actaaagcca cacctccata gagcagcaac
    51961 tgcttccagg gaattgggcg ttttgaaagc cagaaataga ccaggacaaa tacaatcatg
    52021 ccataggtca tttttccggc tatggcaatc cctaacagca ctgccgaaag ccaataccag
    52081 cggcgggggc cttttgaggc gcgttccaat gccagcacag agccaattat agctgccagg
    52141 ggaagcgcct ccaggtacac ctcactggta tatttgattg ccattgaatg aaaagccagg
    52201 aacaggccgg ccagtggatc aatcagactt aagagaaaga cagcaattgt gccaagaacc
    52261 gcagacacaa tgcggttaaa aagcagctct ggtgattccc caaagggggg atcaaacaat
    52321 tggaagggaa tgctgtacaa caatttaacc agcggcggat gctcggcgtt cccctggtag
    52381 ttaacaattc cagaccaatc accgcgagcc atcaaatctg cgtaatcgct ggcggcgcgc
    52441 atatagacgg gttcatcggc atccaggggc aactgccagg ccgcccataa gcgaagcgcc
    52501 agggccagca gtgtcaccag ggcaatggaa taactccgct tccaccagcg aaagtggatt
    52561 ttgaaccggc gtggtttcag tgaaggggcg gtcatttctg ggtcagaacg gcaatataag
    52621 cagcgtaagg ttccagtgag gcgatgggtt gatagccaga caactcgccg ctggcgggga
    52681 ttttcaagtt agacagacgg ccctctccca agaggtcagc cagatgataa tttccagggg
    52741 taaattcggc atttgaggtt aattcaacct gcttaatttt gtcagggctt agatttatca
    52801 gcaagagtat tgtttcgttc tcagttgcat aaatggccgc cagtacccga ttgtcactgg
    52861 tggtcacctt gatatattgt ccctttctca gggcttcgtg ctgactgcgg atggtgatta
    52921 aatttcggta atgagccagc agtgaatcag atgcagacaa ctgagccgtg acatttttat
    52981 caacataatc tttcttgggg ctgatccagg ctgagcctgc ggtaaaacca gcgcctgatt
    53041 cagcggacca ctgcatggga gtgcggatct cttcatcggg cttggtgccg atcatgccaa
    53101 tttcttcacc atagtaaatg aaaggaacac ccggagaggt tagcaggagt accgccgcaa
    53161 tgcgcgcctt atcctcatcg ccggcaaatt cgctcatcac ccggttcatg tcgtgattgg
    53221 tcaagaaggg cgcgatttcg cccggaggga aagtgccata attgaaggag agggcatcaa
    53281 caatctggcg ggcatcagcg ccattgacgc cgcccatgta tgccgaagcc agttcaaaac
    53341 caaaaaccag gtcaaattca tcatttttga cgtagggaac cgcggcaaac agcgtatccc
    53401 agacttcggc aaccgtcata gcctcggggt tttcggtttt taccagtgta taaaaatctt
    53461 tgaaccaggc gtgggtggca tcggtattgc tctgggtttg accatcttca atcaggtgcc
    53521 gggcggcatc aacacgaaaa ccatccacac caacatccga aagccaatag gtggcgacat
    53581 tgcgcatctc ggtggcaaca tcgggattgt tgtaattcaa gtcgggcatg cccgaccaga
    53641 aaatgccgta atagtacccg gcgccagcag agaaatgcca gacatcttga ctccaggggc
    53701 ccaggtaacc ggggtcttgc tctgaccaga tataccaatc gcgatatggc gaggcaggat
    53761 ctttggactg ctcaaaccag ggatgatctt cagacgtgtg attcaagacc agatcaatca
    53821 cgacatggat gccacgagca tgagcggcat ccaggaagcg gcgaaagtcg tccatggtgc
    53881 cgtagtcggg attgacagtg taataatcag taatgtcata accgtgatac gaggggctga
    53941 cttgaatcgg catgagccaa aggccggtga tgcccagatc actggttgtg gcagggtcgc
    54001 catcattaag atagtccaat ttctccgtca ggccattaaa atcaccaatg ccatcaccat
    54061 tgctgtcata gaagctgcgg acaaagatct cgtaaaatac ggcgtcattc caccagttgt
    54121 ctggcattgc agtttgtttg agcgcgacaa catttgacgc aggagtttgc gactgggggg
    54181 aaacgcagga gctcaacaga ctcgataata gaacgaccag cgctaaaaag gcaaccagtt
    54241 ttttatgcat atgaaaatcc ttgtctaaat cagttgtaac agctagatct gccagataac
    54301 gccagccagc gggggaaaaa tgacccgagg atgatgatct gaggcagtaa cggtaacacc
    54361 atactcggat gtcaggagcg tttttctgac aggaagctgc aaattcacct gcatggattc
    54421 ggcggttaaa tttagagcaa caccaatgcg gtcattatca tgctgccggg catataccag
    54481 taaacccggt tgagattgga tgatttgtag actgccaatt tgcagcggca attggttttg
    54541 ccgaagcgca atcagagaac ggtagaattc cagcaaggcc aggtcttgct cctggcccca
    54601 caacattggc aggcgggatt cttcgggaac gccacgctcg ccctggcgaa catcacgctg
    54661 ctgggagagg ccgacctcgg tgccgtagta gatgacgggc tgccccggca aggtgaactg
    54721 gcaaagtgca gccagacgca ggcggcgttt gtcgccctgg gccgcccata agaagcgatt
    54781 catatcatgg ttatccagga aggaggggcg agagaaatcc gctgggaaga aggctgtatg
    54841 gcgttccagg aagcggccaa attcgacacc gtcccaactt ccgtaagcga gggctctgcg
    54901 aagcccttcc agcaagacaa aatctaaaca accatccagt tggccttcaa aagcgatttg
    54961 gacatcgggt gggtcgacga cctcgccaaa ggtccaagac tgggggttgg cggcgcgggt
    55021 cacacgccga aaatcagccc agaagtcggg agcagggcca atagcatagt ccaggcgata
    55081 accatctacc cccaggtcca gccagtattt tgcggcttcc agcaggtgtt ggcgggcggc
    55141 cggattgcgc aggttgatct ggggcaggga ttttacgcca aaaaaggttt catattcatc
    55201 cggccaatga cggaaagtgt accagttatg atatgggctg ttggcatcgg cgattgcatt
    55261 ttgaaacgtt ggatgcagat gcgaccagtg attgggaaca aaatccagca agatgcggat
    55321 tccggattgg tgagcggcag caagcaaggc ggtaaagtct tgtaatgtac ccaggcgcgg
    55381 ttcaacagtg aaaagatcgg tggcatcgta accgtgatgt gagggactgg gaaaaattgg
    55441 gttcagccag atggtattgc agcctagttc agagatgtag tccagtttat ccagaatcgc
    55501 actcagggtg ccgccaaaaa atccgcctaa tgaggcaggg cgctgccagg attgatggct
    55561 gccaggtgaa aagcgatcag ggaaaacctg ataaatgacg gctgattgcg tccaggctgg
    55621 cggcccggct gtttcgacat agaaggcata ataggcgccg ttatccgcaa agatttcacc
    55681 ttgcgccggc gagtagcagc tcaggcggta gcgcaccagg gtatcatcac gctgaccggg
    55741 taattcaacg gtaaaatggc ggatgtagcc ccataagacc gtatcccatt cttcctgaga
    55801 aagctgcatt tcaagcgcat gaccattaaa ggccacgccc tgaacgccga tggggtcgct
    55861 gccatcagtc gtccaatagg cccaggcccg actccctggg aggagcgggc ctgaggttag
    55921 gtgtaattga attgtctgtc cgggaagcgg atggcggggg gaacgctgct gaagatgcgt
    55981 aatccctgag cgtaatttat ctacatgctg gcgccgtaaa gcatcggttg ccagggtacc
    56041 aaagataaaa tcgctcatta acctttcaca ccaccgactg taaggcccga ttcaaagtag
    56101 cgctgcaatg ccagataaac catcaaggtc ggcagcacta cgatcacagc gcctgcggcg
    56161 aagtagcccc agggtacgct gatactgttg gccaaagagt acaaggcagg caccgccagc
    56221 tttagagagt cgggagcagg tacgaggacg ccagcgaaga caaagtcacc ccaggagccc
    56281 atgaaggcga gaatggcggt cacagccaga gccggccggg ccaggggcag ggcgattagc
    56341 gtgaaagcct caaccgggcc gcagccgtcc agcatggctg cctcttccag gtcaatcggg
    56401 atggtatcaa agtagccttt caggttccag gtacaaaata ccagtgtgcc ggttgtgtat
    56461 gcgagaatca aacccaggtg tttgccatac aggcccacgg ccgtgagtag ttgggcaatg
    56521 gccaccagcg aaagcaggcc cgggaaagtg gacagggcca gcagcaggat gaggaaggct
    56581 tcacgaccgc ggaagcggaa gcgggagagc gcaaaggcgg ccgaggtgga aataatcagc
    56641 gagaagattg tggcagccac tgcaactttg aggctgttgg tcaaccacgt caggaagggg
    56701 cgctcaaaca gcatgacccg gtagttttca aatgtccatt cggtggggaa gaacatgcca
    56761 gcaaggttga gggtgtacaa acggtcaccc aagcgccccg aagccagaat ggcaaaccag
    56821 acggggtaga aggcaaagat ggcgaagcca atcagaactg cgtattgcag gatacgcatc
    56881 cagacagatg gtttgcggga cattagcgcg ctcctttcgt aatcttggta atacgcaggc
    56941 tgtagagcgt agccaaaaac agcataatga aaatcaatac agcaaatgcc gtagctcgac
    57001 catagttaga gacctgataa atctgccggt aggcatagac cattacgtac tctgtagcgc
    57061 caggtttatc agcgcccaac tgcgggccgc caccggtgat ggcgaaagcg gtaccgaaca
    57121 tctggaaagc ggtaattgag gtgagcacca gagcaggaac gatggccggg cgaataagcg
    57181 gcagagtgat acccatcaat tgctgccagg ggctggcgcc atccacatcg gcagcttcat
    57241 aaagttcatt ggggacggat tgcagagcgc ccaaaatgga gaccatgaag aagggatagc
    57301 tgaaccagat atctgccagc acaatgacaa cggttgccca gaccgggctg ttcaaccaga
    57361 ctgggccttt gtaattaaac acggccagca atagctggtt tatgacgccc tgatcgcgga
    57421 agaagaactg ccagaccaaa gcaatcaaaa tagcaacagc cgaagcgccc caggggataa
    57481 agagcaccac acgaaagaat gtgcgtccgg gcaaatgctg gctgttaaga atgagagcca
    57541 gaacgaggcc aaccaggaag gtcaggggga cgcgaatggc aacaaacatg accgtccgga
    57601 gaacgaccac caggaagtcg ccagtttcca tcaatgttgc cagggctgca aaaaagtcca
    57661 aaacaaagcc aaggatgacg gctgagataa tggccatcag ccagacccag ccggacgcca
    57721 gcggcggtgt gacctccttg gcaaaacgct tgttgaccgc gtttgcaatg atgaggggca
    57781 ccagacaaac cagcattttg cccagggata ttaacgccgg cggggcaaat aatttgccca
    57841 atagatcggt atagttcttg aagggcgggt ctgcgatccg atatggttcg cccaatccga
    57901 caggagctgt gtctcggaac actggccaac aaagtgggtc taacactcct gaaagagcaa
    57961 ccgagcaatc tggatttggc ttgtacttat tccgggtagt gaaggatgta tagatgttga
    58021 gaatggtggg ataaatattg aaaatcagaa ctgcgatgat tgtgggcagt agaaagagca
    58081 atacagtaag tgcgcgtcgc cgaccggacc agacagacat atttcacgct cctcccaggg
    58141 atttttacga aaaagcgggg agagccttgc gactctcccc gcgacagccc aaaattactt
    58201 catctgggcg acggcttctt cagccgcagt ctgcgcagca gccatggcat cggcgggggt
    58261 agcagcaccc tgccagatcg cctgggtagc cttgccgacg ggatcccact gagccgagga
    58321 gtacggcgag ggggacatgg ggacgcccaa attcagggcg gcgccaaaac cagcaaccgc
    58381 gggcagagcc tggacttcgg ggtccaagag ggcttcggta gcggcgggga tggtcttgtt
    58441 gatgagggag agtttcttct gaacctcagc accggtgaag tatttcatga tgtcgatgac
    58501 aacatcctga ttgccacgat ccacagcgtt tttggtcatc atgagggtct tgatgccaac
    58561 aaacgggcga ccgaagggca gaatgccgta gtcgatacca gattcttcga tgccagcgac
    58621 ggcccacggg ccagtccacc acataccaac tttgccttcc ttgagggcgg cgaagcagat
    58681 atcgtagctg ttttcggcca gggagacatc cttgaatttc aggagccact cggcggcggc
    58741 aacggcttcg ggggtgttca ggttggcttt accagtttca tcgatgtagc tggggacgcc
    58801 gaaaccaaag aagatggggg acatgtggta ggcatcgctg ccaccgaagg cctggttgca
    58861 gaccaacacg ttgccggtat cttcctggaa tttctcggcc ttggcgtaca ggtcatcgaa
    58921 attcatggga tcggtgggca gatattcatc ggtcacgatg gctttattgt agatcagagc
    58981 aatgccttcc tgggattcag ggagggccca gatcttgccc gaccaaacca caccattgac
    59041 gcccgcgggc tcgtaggtgc tggtcaggaa gtcctgggtg atgccataat cattcagggc
    59101 aacgatgttg cccttgaggg cctgttcacc aattttgtca ttggcccagc cgatgatgtc
    59161 ggggccttcg cccgcgggga tggccacatt cagggcattg gcgacatctt cgggtttgga
    59221 gaggtcaatg gtgacattgg ggtgagcggc agtgtaatca gcgaaagcct gttgaatggc
    59281 ttccagatac ttgccatccc actgatgcca gatggtgatg gtcacattct tcacctcggt
    59341 gggcttgggc gtgcaagccg ccagggcgaa ggcgacgatc agcaggatgc tgatcacggg
    59401 gaacaacttc ttcgacatgt tttcctcctc aaaaggatgt gaatagaatc ttacaaaagg
    59461 tcccatttgg gaaccctttt aacaagggga aggtggagaa ttcagaggtg ttttttgtat
    59521 ccctcgaaag cacctccttt ctggaaaaca cctgttctct tagggcaggc tcttcaacag
    59581 attgcataga ttgcatggta ctattgtaat ccatcagaca agattgtcaa gcattaaatt
    59641 tagtgaaagt aatacaaatt ggttgtatga acattatagc acattgcttt tcaggtataa
    59701 tctggcgata tgaatgatca atggcgactg gtgcagacac aaccaagtga tggcgcctgg
    59761 aatatggcgg ttgatgaagc gatcctcgaa tctgtcagcc ggggtgattc gctgccgact
    59821 ttgaggctat atggctgggc tcccccctgc ctttcgctgg gctatgcaca accgattgcg
    59881 gatgttgatc gggaggcgct gaaggcgcgc ggttggggtt tggttcggcg cccaaccggt
    59941 ggaagggcga ttttgcacac agatgaattg acctatgctg tgattgcgcc agtatcagaa
    60001 ccccgtgtgg cagggagcgt gctggaaagc taccgacggc tttcacgggc gctgctgttg
    60061 gcactgcaga aactgggttt gcagcccaac gccgaccgcg aatatccaca aagcaacgtt
    60121 ggtgggccag tttgctttga aaccccatcc aactatgaga ttacgatttc cgggaagaaa
    60181 ttgattggct cggcacaggc gcggcggcgc gagggtgtat tgcagcatgg ctccctgccg
    60241 ctgcacggcg acctgaaacg cattacccag gggttgatgt tcgttgatga ggcagccagg
    60301 ctccaggctg gcgagcggct gcttgaacac gctacgacag tagagactgg actggggcga
    60361 gtgattggct gggaacaggc agccagcgct tttattgaag ggtttcgaga agcattggct
    60421 attgattttg taccctctga attgactaca aacgaaaggg tgcgcgccga cgaactgacg
    60481 cgcacccaat atggcaacga tagctggaat gcccgaattt aatcagggcg cgggcgtggg
    60541 tgtcggggca acaaagataa ctgtgttata tcccagactg cgcagtaagc gcgtcatgta
    60601 gttttcggcg ttttgctgag cctgctctaa aatgccatct tcaatggcgg ctttgtagat
    60661 ttcagcttcg gcgacctgac gggcctgggt ttccaaatcg gtagcgccat gggtaaaaag
    60721 gcccgtctca cggtcgtaaa cgtaggattt ttcattgtcc aaagtggcga caaaaatttc
    60781 agctggcggc agtttcacat acagagtttc gccttcaagc caaatatcct cggccttcag
    60841 cttctccaga tcgatgccag cgatgacgct gccatgggcc acaaaaagga gcttatcacc
    60901 gaacaggaaa gctaactctc cctgcccaat ctctgcagta atcacttttt caaccgtgta
    60961 ttcaattgtt tcgaggcgag ccagagagcg aacttcggtg atgatggtca cggggtcggg
    61021 cagaattgtg ggggtcgggt tgaggagatt tgatacctgg gtcttcaact gatcattggc
    61081 ctcattcaag ggtgaaagcg cctgatcggt tgcagagcga ataaaccagg tgagccccag
    61141 aaaaagggcg cccaagatta agaccaaaac caggattgcg ccgataaaac cagatttacc
    61201 ctgagaaccg ctcattttca aacctcctga atcagtaaat ggatgcgttt aaatggttat
    61261 aattcacaat atgaaaaatt gcaaggcaaa ctcattgtat tatgccatag ctgccctcgg
    61321 gatactcatg gcgctggtag cttgcacaaa gcagccgacc aatgatagac ccacggcaac
    61381 cgcccaggtt tcgcctacgc ccaccgccac accggcacca ttgggaagtg ttgaaaaccc
    61441 ggtagtgatg ggttttgttg agccaacaac cggttccggc aattatcctc cagagcaggt
    61501 tgagctggct caggcaatca cggtcaatac agggttcact gtatcgagcc gggtatatgc
    61561 caactgggag gaactgctcg aggccatgca agacggtgag gtgcatctgg cgtggatgca
    61621 gccactcaca tacctctatg cgagtgagga aggaattgcc gagataggac tgctttccaa
    61681 tcattttgga ttgtattact atggtgtgca gtatctggca aacatcgaaa gtggttttac
    61741 agtttactac gacgcggtca gccaacagaa tacagccgat gcggcaactg ccctggcaca
    61801 atttggcggg cggcgaccct gctggattga gccaaaatcc atttcgggat acgttgtgcc
    61861 agccagtttg ctggcagaca ccgaggtacc aacgctggat gggacctatg tgcaaacgca
    61921 cacggcggcg gtgcgtgctt tatacatcaa ggggatttgt gattttgctg ccacatttgc
    61981 catttctggc gatccgcgca cggcttccgg ggtgctggat gatctgccgg atgcgttgaa
    62041 ccgggtggtt atcatctggc gaacggatgc gagtattccg agcctggggc tggcttatgc
    62101 acaaagcctg cccgagcaat tccgacaggc gatcaatttg acgctgactg atctggtcaa
    62161 aacggatacg ggcaaggcta tcctggcagg cgccaatgat ggttatgata ttcaagatct
    62221 gcgagttgtt gatgactcta tctacgattc gctgcgagaa aatgtaagtg cgctttcgta
    62281 tgatctagaa aacgcactgg gacgttgacg attgaaacgt ggtaaattac gttctgtgcc
    62341 tcagactggg attcgcccac cgcgtaagcg cattaactgg cgtagattgc tacgaacggg
    62401 gattaaagtg acttttaaac tggtgctcta cttgatcctg gctggcatga ttattttggg
    62461 gctgccgcga atcattgcgc tcatgtacgc caagcccagg ttgtacacat ctgttgatgt
    62521 gcccgtatat caagcggcga ttgtttttgg tgccggattg caacgggatg ggacaccatc
    62581 accagtgctg cgagaccggg ttgccactgc ggcggacctt tattttgagg gcaaggtgga
    62641 aaagctgctg atgagcgggg ataatcgctt tgttaactac aacgagccag gcgcaatgca
    62701 ggactatgcc atttcgctag gtgtgccagc ggaagacatt gtactggatt atgctggacg
    62761 ccgaacctac gacacctgct accgggcgca tgccatcttc ggattggatt cagcagtgct
    62821 ggtgacgcaa ggataccacc tgccaagggc attaatcctg tgcaacaatc tagggcttga
    62881 atcagttggg gtggcggcag accgacgggt ctatcatggc aggtcctatc tcatctggca
    62941 gatccgagaa cttccagcta cgctggtggc attatgggaa gcctgggtga cgcgcccatt
    63001 gccggtactg ggcgactatg aacccatttt tccagacagc aatccttgat tgacatgaaa
    63061 tgaggtaaca acaatgaatc gagatgaagc cttaattttg gtaaaagaat atgtgaaaaa
    63121 cgaagggtta atcaaccaca tgcggtctgt ggaggctgct atgcgtttct atgccgaaca
    63181 gatgggacag gatgcagaat tgtggggttt agctggtctg ctgcacgatt ttgactggga
    63241 gatccacccc acggcggagc aacatccgct ggatggcgag ccgattctgc gggcgcgtga
    63301 tgttccagaa gacatcattc aagcaattct gagtcacgca gaccatacgg ggctcccccg
    63361 cacaaccctg atgcaaaaag cgctctacgc ctgcgatgaa gttacaggac tgatcaccgc
    63421 cgttgcgctg gtgcgccctt cccgctcgct ttatgacctg accgcctctt ctgtgaaaaa
    63481 gaagtggaag gatcgtgctt ttgctgctgg cgccaatcgc gaagagatgg aaaaaggcgc
    63541 ggctgagttt ggtgtggatt tatggagcca cgtcgacaac gttattctgg ctatgcgaaa
    63601 aatagcgccc gaaattggcc tggtcggcac gcttcagccg gaagcctgac ccctacagtc
    63661 tcatggctta cgccgtgaag tcggtgcggg tttgagtttc ttcgcgtcgc cagcagcaga
    63721 ggcgggtttc tgcttttttg aagccggctt tgaggtcgtt gtatctgctt ttgacacagg
    63781 cttgggtttg gctgccggtt tagcggtagg tttggctaca ggggtttttg gcgttgcggt
    63841 tggtttggaa ctggccgccg gtttggcctt tgaagttgtt ttgggtttgg cggtcgatgt
    63901 ggatttctta gaagccgggg aggccggggg attcttcttt gcaggggcgt ttttttctgc
    63961 cggggccgaa cctttttcag ccgtgcggcg aggtttgcgg gttggtttga tttcagattc
    64021 ccacagagcc aggccatcag agatcgcggt caaattaagt acggcatcac ccgcttttat
    64081 tggagccagg tcctggccct tcatcaaacg cttgcccagg gggacttcat ccagccgggt
    64141 aagcctggca gcggcctgct ggaagtgaac caaggcagtt tgcgtacgtt tgccaaaaat
    64201 tgcgcctacc agacggttgg agccggtcag cttgcaggcc aagacgccct ggccatagcg
    64261 cccttggagc ggaacgccat ctttctcaat tcgccacccc aagccttcag atgagacaaa
    64321 caggatttca ccttcaccag acaggcgttc tgcggcagcc acgcgatcct tggtctccag
    64381 tttaataccg ttgacacccg ctgcgaccag ccccattgga cggacttctt gctcagagaa
    64441 gcggatcagc attccgagtg cggtgaccag cagaatttca gaactgccat cggtcacaaa
    64501 ggcccagccc agttcgtcgc cagcattgac gcggctcaaa acaaaaccct gcgctgatgg
    64561 gccagagaga tcgctgacca ggctcttctt aatcatgccc agtcgggtaa ccgtaacaac
    64621 ataccgctcc tggccgggtt caagacgttc gggaattgag aaaatagctg ccagcgcatc
    64681 atcggcatca aaaggtgata ctttgttgag cttgacccca tcggcgaatt tctcggcttc
    64741 aggcaaagca tgtacagcta ctgcggccgc gcggccggtc tgggctacca gataaagagt
    64801 gtggtgggtg ctggttcgca gtaaccagcg cgcagcctga cggccgctaa tgcgtgggag
    64861 ttcatctccg gaggtgcggg caattacgcc atcttcggtg acacccaccc atacaacctc
    64921 tgcgggggtg agatcggtgg tcgtcagttt atcggcagcg acctcaccat ctgccaggga
    64981 aacgatctgc gttcgacggc gatcgccata ggcctggcgc atggccatca attctgtctc
    65041 caccgccccg cgcatcttct taggtgactt gagcaggctt tccaattctt tgattaatgc
    65101 ctggaggtct ttgtattcct gctcaatttt cttacgctcc agagcggcca ggcggcggag
    65161 cattaaatcc agaatcgcct gagcctgaac atcgctaagc ttgaattttt tcaccaggcg
    65221 ggcatgggcg gtctcaacat ctggggcgtt gcggatgagg gtaataattt catccaggtt
    65281 gttaatggct atacgcaaac cctccaggat atgagcgcgc tgtttggctt tggcaagatc
    65341 gtattcactg cgccggcgaa caacttccag gcggtgctcg atatagacgc gcaaggcttg
    65401 cttaagagtc agcaggcgcg gctctccatc taccagtgcc agcagtgtga gactcagggt
    65461 ggtttccagg ggggtgcggc ggtagagttc acgcaggatt ttctgctcat ctgcgccagc
    65521 cttgagttcg atgacaactc tcatcccctg ccgatctgat tcgtcgcgca aatcagcgat
    65581 gccttcgagg tcgccttcgc gagcaagctc ggcaatgcgc tcaatcagag cagatttatt
    65641 gacctgatag ggtaattcag taatgatgat acgatcacgg ccacggctca tttcttccag
    65701 atgaacacgc ccacgcaaaa taacccgccc acgcccactg ccataggcgc ttaataaatc
    65761 attgcgatca tgatcttgaa gaataatacc acctgtgggc agatcaggac cctggataaa
    65821 tttcaccaga tcagccacgg aaatgtcttc catctgctcc cactggcgca gcataaagat
    65881 cagggcatca acgacttcac ccaggttatg gggtgggata ttggtagcca ttcctacggc
    65941 aatgccgctg gcgccattga ccaaaagatt aggaatggcg gctggtagta ccgccggctc
    66001 gctaagggta tcgtcaaagt tgcgggtaaa gtcaaccgta ttacggtcta attgcgccag
    66061 aagatcaatg gcgtaggctg ttagacgggc ttcggtgtaa cgcatggccg ctggcgggtc
    66121 gccatccacg ctgccaaagt tgccctggcc atcaaccaaa gggtagcgca tggagaaggg
    66181 ctgcgccaag cgagccatgg cttcatagac agctacatcg ccatgggggt gatatttacc
    66241 cagtacttcg ccaacgatac gagcagattt tttgtaagcc gaatcgggac gcaggcccat
    66301 atcatacatt gcatataaga tgcgccgctg gaccggtttg aggccatctc gggcatctgg
    66361 gagtgcacgt gagacgatga cgctcatggc gtaatcgagg taagactgct gcatctcgtg
    66421 atcgatatct atcttgcgaa ccagaccaac ttccatatcg cactccttgg agaaattatc
    66481 ccctctattg tatcaggtca ttaaaacaat ggggcgcggc gcaccatcct aaaatggttc
    66541 gaccgcgccc caatttgcat tttgttatat gttatgcttc ttcagggagt tcagcgtccg
    66601 cttggggtcc ctcttcggca gcctgggtgc ggaatcgtcc gtagacaaag cctgtgcccg
    66661 ccgggatgag cttgccgata atgacattct ctttgaggcc aaagagcgga tcctcagttg
    66721 aggcaatggc agcacccgcc agaaccttga tggtgtgctg gaaggaggcc gcagagagga
    66781 aggaatcggt gctcagggag gccttggtga cacccagcag gatctctgca taacgggcag
    66841 gctgcttgcc ttcggcaatg agctgctcgt tgacgcggcg gatctcaagg cgatcgacaa
    66901 ggtcgctggg caggtagccc gagtcgccgg ggcggatgac ctgaacacga ccaaccatct
    66961 tgcgcaggat aacttcaaag tgcttgtcgt ggatgttctg accctgagag cgataaacct
    67021 tttggatttc ggtcatcagg tacatctgac aagcctcgcg gcctttgatc cgcagtacag
    67081 tgtgcggatt gagcgagccc tcggtcaacg cctgaccagc ctcaacatgt tcaccatctt
    67141 tgatcagcag acgggaagtt gtcggaattt cgtattcttc agtttcgcgc tgttcataag
    67201 acacgatcac tttacgatct tcgaggcgaa cgcggcctgt atgctgagct ttgaccacag
    67261 cctcgccctg ggtggcaagt tcgtcgccca cagagatagc atcttcatcc ttgacacgaa
    67321 tttcccagcc ttcggggata tcgtactcat cgctgaccat ttcgctgtga tccacgcgaa
    67381 ccatgcgctg gtcgctgtag cgctcattga caatgaccgc aaccacgcca gcaatgctgg
    67441 tgataacagc ttcacccttc ggggcgcggc gagcttcaaa cagctcttcc acgcggggca
    67501 gaccggttgt aatatcactg ccggcagcca caccacctgt gtggaacgtg cgcagcgtaa
    67561 gctgtgtgcc gggttcacca atggactcgg cggcaacgat acccacagca gatccaagtt
    67621 caaccatgcg gccgcggccc aagtccatgc catagcattt ggcgcagaga ccatgcacca
    67681 gttcacaggt cagtggcgaa cgaaccttga cttcgtcaat gcctgcctga cgaatgcggc
    67741 ggatcaaatc gtgacccagc atttcatcgc gctcggcaag aacttcgccc gtcaagggat
    67801 caacaatgcg ggcggccgtc aggcgaccaa agaggcgcac agacatggac tgtccggcga
    67861 tatcgtcgct cttgcgaatc cagaggccgt cgtgtgtgcc gcagtcttct tcattaacaa
    67921 tcatatcctg ggcaatatcg accaggcggc gggtgaggta accggcatcg gctgtgcgga
    67981 gcgcagtatc agccagaccc ttgcgcgcgc cgtgggtaga gatgaagtat tccaaagcag
    68041 tgaggccctg gcggaagttt gactggatag gcagacgaat gatgcggccc gccgggtcag
    68101 ccatcaaacc gcgcatacca gcaagctggg agatgggacc gaaaccgccc ttggtcgcac
    68161 cagagttcgc catggtcgcg aggttgccat tgggatccat gttgcggcga acagcggagg
    68221 caacatcctt ggtggtctgc tgccagattt caatttcgcg ttcgtcacgt tcctgctcgg
    68281 tgagcagacc acggcggaag gagcgttcta cgttttccac atcgcgctga gcctgggtca
    68341 ggatttcgtc tttttcagca ggaatagtga tatcggagac tgaaagcgtg gagcccgagc
    68401 gcatggcgta ttcaaagcca atggccttga cgttgtcggc gacctcggtg gtgacttcct
    68461 cgccacaata ctcataaact tcggcaatca aatctttcac accgcccttg tcgagcacat
    68521 cgttgacaaa gcggatttcg ggcggcagga tgcgattgaa gagaactcgg ccaaccgtcg
    68581 tctcgattaa tttccgaatc ggagagggca aacgatcgcc attgtcgtcc aggttggttt
    68641 ccatcatcac gcggatattg gcgtgaatgt ccacctggct caattgatag gccatttcga
    68701 cctcatcaat atcggcgaag acgcggccat caccagaatg aggcagatcg ttgcgcatgg
    68761 tcaggtagta gacacccagc accatgtctt tggaggggct aatgatcggt tcaccatcgg
    68821 caggtttgag caggttgcgg gaggccaaca tgaggtttcg ggcttcccat acagctttct
    68881 cggaaagcgg gacatgcaca gccatctggt cgccgtcaaa gtcggcgttg aaggccgtcg
    68941 ttactaacgg atgcaattgg atggcactcc cctcgataag caccggttca aaagcctgga
    69001 tgcccagacg gtgcagggtt ggggcacgat tgagcaacac agggcgttca tggatcactt
    69061 cttccagaac ttcccaaact tcgggacgat tgcgttcaat aaagcgccgg gcgcctttga
    69121 cgttggcggc gtagttgtgc gagaccagac gggaaatgac aaacgggcgg taaagttcca
    69181 gggccatagc cttgggcaaa ccgcattgat acagtttgag cgtcggaccg accacaatta
    69241 cagaacggcc agaatagtcc acacgtttgc ccaacaggtt gcggcggaag cggcctttct
    69301 tacccttgag catgtcgctg agggatttga gctcgcggcg gccgcggcgc gaaagggctt
    69361 tgccgcgctg agagttatca accagggagt ccacggcttc ctggagcatg cgtttttcgt
    69421 tgcggacaat gacatccgga gcgccaagtt ccaataagcg cttgaggcgg ttattacgat
    69481 tgatgacgcg gcggtagaga tcgttgaggt ccgaggtggc aaaacggcca ccatccaatt
    69541 gaaccatggg gcgcaaatcg ggagggatga ccggtaagac cgtcataatc atccactcgg
    69601 ggctgttgcc agagcggcgg aaggattcaa caaccttcag gcgggtggta gcctttttgc
    69661 gcttctgctt gcttttggaa gtgcgggctt cgtgccagag ttcttcggaa agtttatcca
    69721 gatcgaggcg gtgcaggata tcatagaagg cttcggcacc catatctgca cggaaaacct
    69781 ggccccagcg agatttcagc tcgcgatagc ggctttcgct caggaaggta aaggggcgca
    69841 gctcttgaag ctcatcgcgg aaatgggagt tttgattttg agaaaccgag gactgatcct
    69901 ccaactggtt gcggagggtt tccatttctt cgtcagcctg ggcgcggact tccatgatgc
    69961 ccattttgat ttgttcaagc tcgcgggcga actcatcacg gaattcagat tccaactggt
    70021 tcaggcgttc tttaacaatc tgttgaacct tggtcatgtg ctgggcggta atggtttcgc
    70081 caacaccaac aatttcctgg ctggcagcgg aaaatacaag cgccgagcgc agggtctgcc
    70141 cctgtttatc ggtcagtgtg cgctccagcg attgaccttc ctggatgacg ggttcgagac
    70201 gggcggcaat ctgctcgtcg cggcgttctt ccatttcggt tttgcgctgc tgcagttcgg
    70261 cgattttccg atcgcgcgac tgtttgactt ccagaatgcg ggcattgacc tgggtggcct
    70321 gctcgcgctc agaaacactg atttcatctt caaggcggcg caaagccttc tggcgggcat
    70381 cctcgtccac ataagtcaca atgtactggg cgaagtaaag cacgcggtcc aggttacggc
    70441 gggagatgtc gagcaggagg ccgagatagg agggcacacg ccgggtgtac cagatatgcg
    70501 ccacaggggt agcaagatca atgtgcccca tgcgttcgcg gcgcacggca gagcgcgtaa
    70561 cttcaacacc gcatttatcg cagacaatac ccttgtatcg agggttttta tatttgccac
    70621 aatagcactg gtaatcacgg gtggggccaa aaatggcctc acagaacaac ccgtctttct
    70681 cgggtcgcag ccgccggtag ttgatcgtct cgggcttgag gacttcgcca taagaccagg
    70741 atcgaatcgt ttctggcgag gccaggttga tgcgcagtgc gactaagctc ttggtttcca
    70801 cttttcagcc tcctcaatgc ggttcagtcg tataaaacaa agcgagcgct cgagaagatt
    70861 ttcccagcgc tggaacgaat ttcagggcat tctaatagcg tacatgcaaa cgagtattat
    70921 aacacgactt tttccgaaat accagcacta attttcgatt tttggatttt gcttgggttt
    70981 tttagcagca ggctgctcgc ggggtatttc aaagaagaag gcgctgccct tgcccaactg
    71041 gctttccacc catactcgcc caccgtggcg gtcagcaatg gacttgacaa tcgccaggcc
    71101 caggccggtg ccgtgctgtt gataggcttc tcggcggccg ctgcgataga atttttcaaa
    71161 caggcgcggc aagtccagcg gggcaatgcc aatgcctgtg tcaatcacgg agaagagaat
    71221 ggtttcggcc cgcgcctgga gatggacgcg cacccgccca ccgacggggg tatatttgat
    71281 cgcattctca accaggttgt agatggattg ctgtaacaga gcgctgtcgg cttctatcac
    71341 caccgggcca tcttcgggca gttcggagac aatctggatg tttttctgcg ccgcttgagg
    71401 ctgcaaaagc gcaaccacct gactaacgat ttcgttagcc cgtatatttt caagccgcag
    71461 gtcaatgcca gcttcaattc ggcccagatc caagaggttg ttcaccaggc gcgtcatatt
    71521 gtcaacgccg ccaatcatct tcttgacata gccttgctgc tgctcgttaa gctcgccaac
    71581 catttgcagc atggtggcgt atccccgcat gagggtcagg ggagaacgta aatcgtggct
    71641 gacagtggag acaaagtcgg atttcagtgt atcgagttct ttgaaatggg tgatgtcgcg
    71701 taacatgcaa attttaccga cctggcggcc atcagcaata accggcgaaa cgctggcata
    71761 ataaatccga ccattttgca gcgccagttc gcgtgagacc aggccgcttt cgggtggcgt
    71821 ttgcagcaaa tcgagcagtt cgggcatcag aacgacttcg ctggcgggct gcccgggcat
    71881 agctgaagca attagcccgg ggacctgtag aactgccggg ttgaccagga aaaggcggtc
    71941 tttctcatca aatacaagca cgggctcggg ggtcgaagcc agaaccgctt cgaggcgctg
    72001 gcggccaatt tctgaggtgg catacagccg ggcatttacg gcagcaacag cagcctcgct
    72061 ggacagtgtg ctgaggaagc ggacttcgga ttccgagaaa ttatgaggcg attcgtagcc
    72121 aacccagaga accccgtaat agtagttttc ataatacagg ggcagggcaa tcagcgcacc
    72181 cgggaaaagc tgaccgggtt cgggcgctac gcggcggacg cgcagagtgt tggggatggg
    72241 cagcaaatct tgatggcgca tcaggtcgaa gatttgtttg tccaggtagg cataggtctc
    72301 agttgcgggg ccggcgccaa aagataccca gggggattcg tgcgtctcca tcataacatc
    72361 ccggattagt acgatccggg cgctatttga atcgggtccg agagctgctt cgagaatggc
    72421 actgacggcc tcaccgacct ccaggtttgc cacgccctga ctgaccacca acaggcggtt
    72481 cagttcttcc aggcgcgcct tgaggctgat gcgcatttgt tcaaatgaag tgcccaggcg
    72541 cccaatttca tccacgccgc gaactttcat agggttatca aggtggcctt gagcaatttt
    72601 gaccgattgg ttggccatga ggcctaaaga gccggtaata gagcgcaggg tcagccgcag
    72661 tgtggaatag gccacaacaa cgaacaaagc caatatgagc agtagtggaa tggcgatttt
    72721 aagcgcaagc tgatttgcac cttcggcagg catggtcagg acaatcgccc aggggcgccc
    72781 ctcgcctggc tgataataga ccaattgacg ggtgccgctg gcagaaacat cttcaaaaaa
    72841 cgcagcttcg gtttggggtt ggccaatata actgctcatc accaattcgg gtgattgcat
    72901 ggtatcgtac aaaatgcgtt ggttctcgtc aagaatcatg ccttcgccgc cttcggcggc
    72961 aattgtctgc atggcttcaa tggctggctg ggtatatggg ttggatacca gatctgtgcg
    73021 cccaatcaat accccaatca tttgtccggt ctcgctttga attggggata tgaatgagat
    73081 ttgtgccgag attgggtttt ggtcggccgg ttgggtatag gccggaatgg cataggtttg
    73141 aatcgggacg ccctgcaagg ctaattgaac agccataagt tcttcaggcg ccagcttgat
    73201 atttgccacc cgagattcag gataccccga aagaatggcg ccagtggaat ccactaaaac
    73261 catctgtctg aagtagggcg acaaccgaac ctggtcggcc agacgatcgg cctgagtctg
    73321 aggcgaaagc gggatcaaat cgggggtggc ataattttga accaaagttt gtccggcctc
    73381 caaaaagtat gggaggcttt tggctgctac ttcggcagcg ctggacatgc gcccgttgat
    73441 catctgccgg gcagcggagc cagccaccca ccagtcgccc agggttagaa ccaaggccgt
    73501 gatgataacc agcggcatga tgctgaagaa aaatctccag gagaggctgg attcggttgg
    73561 agagggctgc agaactgcgg ggcggtacca ccccatggct gggaacagca ataagatttg
    73621 agcaacgatg ccggcgatca ataattcgcc tatgcgcgcc aagaaggaga gccaggactg
    73681 cgtcagggca tagtccatac gaacggcgat agaaccgttg acggtaaaga aggacgacaa
    73741 caagtaaaac ggcaggaaga tgacaccaac tgccagaaca gctatcagcg gttgtcgcag
    73801 caaccggtag aaagttgtgc ggtagggctg gcggatcacc atggcgaaga ccagagccaa
    73861 accggtaatc tctatagggg taaataagtt atgggtgccc catagagaca ggatgatgcc
    73921 gctaatcagc ccaaccagag acgccggaat gacgccgagg aaacccgccg ccagaaccca
    73981 ggggagggca aaaaacatga tcagcaccgg agcggctgca tcgcctggca tgttaggcaa
    74041 tgggatgccg ctgccagata gctgcgccac catcaaaaag gttgcgaagg gggtcagggc
    74101 aatgaaagct gccagcaagc cccagcgcag gtttttgaaa gcgccgggag tccaggcaag
    74161 ccgaacaccc cagaagagca ctgccagcaa agcaaaccag ccaagccagc caagtaatcc
    74221 tccaggccag atgatattca gatttcccaa ctgtagagta gtcattgagg gcatgggaga
    74281 agtggttgca agaggtgtac cagaattata gcatcaggcg attaatttat ggtctgattt
    74341 cagggttggg tttcggtaag ctgggtgatg ggcagatata tagagagctg tgacagttct
    74401 tcggtcagtt caatggttaa gcgcccgcga tacaattcca tcaattgggt gacgaccatc
    74461 aaacccaggt cagcggcagg gtcaagatcg tttcgattta atgcggggcg gccataaaaa
    74521 gagatctgga cgcgatattc ccacccgatt ttttgaccta cgatcgtgat ctgcgaatga
    74581 ggcagcgata agcggagtgc ccggtcgagc aattcttcga ccaccctttg aaagtggctt
    74641 tcctggatgg cgagattgac actctctgat tcaatcttga tgtcttcttt acgatcgcca
    74701 gacagcgttt tgacggcgac caattcccaa atcagattat gcgcttcaag gcactggcac
    74761 tggcgataga tgcttagctg ttcggggttt gccaaaacca ggctcaattc gctgaaaatg
    74821 cgataatttt ccagaacgcg ggcgatgtgc tgggcagaat tttgaatttc gcggccaatc
    74881 tggcgaattt gactaccttc caacggctca gtgctatttg ccaggaggtc agaatagccc
    74941 aataatacgc tcaaaggagg cagcagatat ccggggaccg aaaaagacct ctgccgtacc
    75001 atatcactaa agcgggcatc aatcaggtct ttcatcaagc gccgcttttg cagacgggtt
    75061 tgcactgccg ttaataattc ggcgttgtta tagggcttgg tgatgtagtc gtcagagccg
    75121 agttccatcc cctggcggat ttctgagcgc ttatctttag cggttagaaa tataaaagga
    75181 atggcagagg tgtttggatc ctgactgagg attttgagga cttcatagcc ttcaagcccg
    75241 ggcatcataa tgtcgcagat gattaaatct ggtgcgtttt cccacaccag gcgcaaacct
    75301 tcttcgccat ttgccgcttc aaggggttcg taggcattgt ttttcagcag aatggcgata
    75361 ttcttgcgga tctggtcttc gtcctcaata attaaaatct tagggggcaa ttgttaccta
    75421 cctgtaagct gcgtggggaa tgtgacaact gcgcgggttc cagaattcag gtgactatcc
    75481 aacgtaatat cgcctcccag gctgtttgca gccagacggg caatcgtcaa gccgaggcca
    75541 acaccgggaa ttttgcgtac gttacgccca cggcaaaagc gcccaaacac ccaggggatt
    75601 tcatcggcag ggatacctga gccctgatcg gtgattgtca ggtgatagca attcaagccc
    75661 gatgacaatt ctacaagcac aggcttaccg gggacggaga atcgaatggc attgagcagc
    75721 aggtgcaaaa ctaccagata aaataagtca ggtggagctt gctcagtaaa atcaggcgcg
    75781 ggaagcgcaa agttaatcgg gtggttgttc tgatcccatg cggaaacatc agcaatggca
    75841 cgccggcaga tggtgataac atcttcacaa ggggatgctt tggcaaccag ggttccatcc
    75901 agcagttgca gatcaagaac gtccgaaacg atttgattca agcgctggac ggcagtatgg
    75961 atttgttcga gctgcgacat gcgtccatct gaagaccagg attcgccata ggcaattaac
    76021 agctcagtgg acgatagcgc aattgtcagg gggttacgaa actcatgaga tacagcggag
    76081 gtgagacggg cttttaccga ggcgatttca tgatcagttt gagattcccg caaggctgcg
    76141 gtaagagcca atgcaagata ctggttgatc aagctagagg gaatgtatcg attggcacca
    76201 gcatccagca aggtgtttga gaaggcaata gattcggatt caccaactgc aaggatgggc
    76261 agatacagcc cacgtgagcg aactgattga atcagttgaa ctgctgatgg cgcatctgaa
    76321 tcaaccgata aaatcagtgc gtcccaggtg tcggtttctg cgttgagcgg agcattaccg
    76381 gacaatattg accaaaccgg cgtcattgta aagccgcccg cagcaagcgc atttagaatc
    76441 tgctgcgagt cggcgtcatt caaaaaggcc agggcaaggc gaaggttatc catctccagt
    76501 agcgcactgt tctacaatct cacaggctcg ggcaaagacg gcatctgagt ccagaccatc
    76561 cgaatccaga atcacagcat cgtcagccgg gcgaagcgga gcaaccgccc gggtcgagtc
    76621 gatttgatcc cggcggcgca ttgactcaag aatggttgca tagctggcgc gctcaccccg
    76681 ggcaagaacc tcatcagtgc ggcggcgggc gcgttcttcc accgaggcga ccagatagat
    76741 tttcacatcg gcttcgggta aaacaaccgt gccaatatcc cgccctacca tgaccacaca
    76801 acctcgcaag ccaatgcggc gctgctgggc tgtcattgct gatcgaacgc cagaataagc
    76861 tgacacctga gaaacattag catccacctc ggggcggcga atatcccagg tgatatcttc
    76921 gccatccaat aagatgtctg ctttgcgacc atcggttttt gttggtgcgc tgacatcaat
    76981 ttggatttga ttggcaattg ccgtaaccgc agcctcgtca tccacggttt tcagtgagcg
    77041 cagcgctgcc caggtaaccg cccgatacat taccccggta tcaaagtaga ggtatcccag
    77101 tcgcgaagcc aggcgctctc ccagcgtgga tttgcccgat gcggccgggc cgtcaattgc
    77161 aatggttttt ggatagttca taaaatcacc ttgttcaggg agtgggattg attgctgctc
    77221 cagggaatag atcggtacgg acccataaaa tggcataggt ggtgtacacc ggcgcacggc
    77281 gataaaaccc ccattccagc cattctctgg ccaccatagc aggccattcg ggagcagtgt
    77341 aaattgtaaa atcctggccg ctgtagggtg cggttagttg cagtgtattt gtctggtctg
    77401 tgatcactgt cgaaggccgg gcatccacag gtaaaaccga atcatattct gcttgaggat
    77461 agtccttcaa agcccagcga agtgatggtg actcttgaat cacaaccaga tccagaggct
    77521 ggcggttctt ttgcgcccaa tctgaaatct ctgataacga tttgagcatt aaatcttgct
    77581 cggcagggta ggcatcaaca cgccaaagct cacgatccgg gtaattcccc cgcccagcag
    77641 cgttccacat ggctgcaaca gtcagcacga cagccacaat agcaacgccc caagtcaggc
    77701 catttagcgc ggtcgggatg gaccaaccgc cagcgactaa caaggtcaga atcgccagca
    77761 ggatgagtgc agctgcaagc tgggcccaac gcgcaaaaac ctgcgcctca gacaaggttg
    77821 tctgggtgac cagcgccgca aggttgacaa gcactactgt aatcagtgcg gcgactaaag
    77881 cggcctgacc ggcgctgata aagcgatgtt cccgcacaat gccagcccag cgaatggcct
    77941 ggcgcgcggc caaaacccag aggggcagca gagaccagga caggtcaatg acctgccgac
    78001 tggggtaaac caaggccagc agcagtgcaa taccgaacca gaagaaaagg aaccgatcaa
    78061 tgggttctgc tttgaataag ctacgaaaac cgccgaccaa ccccaagaca agcggcaaaa
    78121 actcatagaa gatcagcgcc agaggcaatt gtcccagaga gatgccagaa gcgcgccccc
    78181 agccctggaa atatgtgccc aggctggctg cggcagcgct caaaccgcgc ggttgcagga
    78241 acaacaatgt acccacaacg attaacgtgc ctgcggccca gatgagggcg ttacgccaag
    78301 aaaatggagc aggcgcatca tcagaagaag cggcgataga ggatcgcccg aagaccagaa
    78361 cagcaatcgc aagggccagc cagccgggga ggaaattggg accgcttagc agggcaagcg
    78421 ccaggcagat tccggcagcg gccgatgagc gtctgatgta gtagcccagg gccagaactg
    78481 tacaggccaa gccaatcatc attcccccgg cctgacggga aacagctgtt aaaccaggat
    78541 ccagcgccag accaaaagcc agcaagattg ccgaccagcg ccccaactgc cgccgaaata
    78601 gcaggggcga caagaccagg agactgcctg ccagggcggg gatcaggcga gcggtgaaat
    78661 ctgtagcctg gaaaatggca aataaaacgc tggtcatcaa aatataggcg ggttggggat
    78721 caattgcagc ggtgcgatca ctgaatagat gggcagcctg aagcgccaat gcagcttcgg
    78781 tatcgccaag aggtgtggca gcaagagccc agagccggga aaccagggcc agcagaaaag
    78841 ccagcgcaaa taagatgtgt tcaatggtaa tgggtctgtt tttcatcagg cttgggcctc
    78901 acggggtggt ttgcaggggc actatttcca attgggggac ttcgtaaatc attacggtat
    78961 tggtcacaaa aaccggtttt aggtaggtct ggaagaggtt atcgttaaca ggataggtgt
    79021 tgcgctccaa ggggccaacg taaacatatc gaatattata cctgaccagg atggccttca
    79081 cggcttccca atctgagctg acgtaaagct gctgcatatc gggtgtgcgg ctgccaatct
    79141 catctcgact accgcgccat tggccctcgt ggttgtccca gcccaatacg gtgggcaaac
    79201 ccgtgttgac cgagatgcgc gcataagagg aataggcgcc gccgacggct tcggcgacta
    79261 ccccataggg cgcatcgcgc agccagcgaa tagcagccat ttcatccggc tgactgcgct
    79321 cgatgtgagc agtgccgtcg agcgtgtaat tatcggttac tttaaagtta cctgtgtaat
    79381 aaggcagcat ggttacggga taggtcagag acatcaacag gacaatggac caaaccgcgc
    79441 taaagatgac ggcccggaat ccgctcagcg cctcccagag gacggcagag gcaaaggcag
    79501 ccaccagcga ccagatgatc caggtttgga agtaaaactt gaagatggta ttcatgcggt
    79561 tgccaaagtt gtcccgcagg aagaaaaatt ccgggaacag ggccaggcca gcgcccagaa
    79621 gggccaacag tgcggcaaag gcctgaacag gggtaatttg ctgggatgtt gattcttctg
    79681 aagcagtgcg acgttttaaa gcagacatta gaccccaggc caggacaaca atggtcgtca
    79741 ggggcagcca tgtgccgaca taagttattc tgcgctgcag ggcggcgcca agcagcgcgc
    79801 cgctgtcgga agctccaaac aggctgttaa tcatactggc ccagaatacc atgc
//
LOCUS       DAOPVS010000002        70817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_338_length_70817_cov_58.853735, whole genome shotgun sequence.
ACCESSION   DAOPVS010000002 DAOPVS010000000
VERSION     DAOPVS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 70817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70817
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_70817_cov_58.853735"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..538)
                     /locus_tag="PKL11_00340"
     CDS             complement(158..538)
                     /locus_tag="PKL11_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12071.1"
                     /translation="MGHTILYIEDNPDNMLLVRRVLESRGYTLLSAVNGLDGVQIAET
                     QEVDLILVDINLPDIDGYEVTRRLRSSSKTALAYTPIIAITANALKGDADKALSAGCD
                     VYMSKPINIRELGARVEAFVPSQD"
     gene            complement(657..1049)
                     /locus_tag="PKL11_00345"
     CDS             complement(657..1049)
                     /locus_tag="PKL11_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12072.1"
                     /translation="MENAILCIEDNPHNMLLMKRILEASGYVLFQSNTGLDGIRIAET
                     ENVGVILLDINLPDIDGYEVIRRLRSSQKTGLASIPIIVLSANALVGDDQKALSVGCN
                     MYLTKPVDIQDLRDAVNKYIPAPNSLDE"
     gene            complement(1065..2711)
                     /gene="thrC"
                     /locus_tag="PKL11_00350"
     CDS             complement(1065..2711)
                     /gene="thrC"
                     /locus_tag="PKL11_00350"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ12073.1"
                     /translation="MAKLKYLECVLCGHQQPYVPLVAAVCQQCGSQWLEARYDYVAFK
                     QNILRGLPGRPANLWRYQDILPVEAPAAINLYPAGGTPLWLSQRFAPALGHNTVYIKD
                     ERYGPTSSFKDRQAAVGVAAMLESGVKEAVIASTGNAAVAYAAACARAGIKLWVFMTS
                     LVPQEKLREAALFGAEVIRVSGNYDQTKQIAAQFAQRRQLFLDRGAGSLPARESMKTI
                     AYEIVEQLNWRSPDWYIQAVSGGLGPLGVYQGFKELFSMGLIDRIPKLAVIQTEGCAP
                     MVRAFKAGKDTADPVIPDTCIIILSTGDPGSSYNYLWRLTQQFGGTMESVTDAEAFAA
                     MRSLAKSEGMAVEPATAVAFAGLEKMLRNGIIRPDEQVVINCTGHTFPVEKHVLGDQW
                     AVDVHLSEGQAAAPREGVQAALENLDEKTTTVLLIDDNGDDALLIRRLLEARKKYRVF
                     HAKDGIEGLKMARQKRPDLIVSDLTMPGMDGFGLVEALKSDELTRNIPVVVVSARDIT
                     PEERQRLSGHIEAMYQKGSLPSRKFVEQVVQVIEEEKDKE"
     gene            complement(2713..4578)
                     /locus_tag="PKL11_00355"
     CDS             complement(2713..4578)
                     /locus_tag="PKL11_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNZ12074.1"
                     /translation="MPKKKIDTRLHDLFSGFDRPEAHPETDAAPVQGSDLEAAQPQPL
                     EPAEVLIPASEPAAPAVPEPAAPISLPQAPIIYEREQVGYAFTGNTLQRVRESVLKQV
                     DLKNAILAPLTTSGQPIGAVVVEPPPSQTWTSEEQSLIEAVAQQVSLQIQSLRLLSSA
                     ERARTEAQAATRRFIHESWDAYLDGIHQNERIGYAYDQNGVEPCLDPAPPQVTYQESV
                     EVMDTQVGRVMLQTDEGHALSDDEQALVSAVARQVGQQVENLRLLADAARARAEAEDA
                     SRRLTREGWSGFAQANADLTPGYVYDLNQVSPLTSQPQNVSLSQPLMVRGEVIGELAV
                     AGLNNPSPEAAGLMAEIATQASVHLETLRLNEELRRRAFELQELDRLKSSFLANMSHE
                     LRTPLNSILGFSDVMLEGIDGPLTEYMENDLRLIQKNGQHLLHLINDVLDMAKIESGR
                     MNLHPEKVKLSDVFTEVINITSSLAGEKGLDVFIESTSNTDLEIYVDVTRLRQVMINL
                     VNNSIKFTEKGFVSIRAEEMPDDQIMISVRDTGIGIPPDHLESIFQEFSQVDTSTTRK
                     VGGTGLGLPISRRLVEMQGGRMWAESSNVPGEGSTFYVILPREAKISEPIEVVAK"
     gene            complement(4608..7556)
                     /locus_tag="PKL11_00360"
     CDS             complement(4608..7556)
                     /locus_tag="PKL11_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12075.1"
                     /translation="MTHTTAANSSKKPLLSNRGIRARLTALVMILTIPLLIGVAVYIS
                     SRAGSEIQNQSLLHLEEKTLSLSTTVSTWLDSQTRSLKQTTMLPSITAMRATQQKPVL
                     EVITQAYPNLFLVHTLDLNGLNVARSDAGAQTDYSDRAWFQAARGGTPVTYEVLISRT
                     INKPALSMSAPIYSGDQIVGVVGYTSELTEISDEVVSTEEGGTITFIVDANGFMVAHP
                     DPTYTTEKLADFSAYPPVAALIRGETGQLTFTDDSGVKWNAYAGRLENGWGIITQQPN
                     AELLAPVRQFQTVAGILILLGSLAMAILVGIAIQRLLRPIGLLTDTVTAIAGGDLNQV
                     AEVVSQDEIGVLATAFNNMTGRLRESFTTLEQRVAERTQSLELAAEVGRSVSQVRALD
                     VMLKDAAELIRSRFNLYYVQVYLTDPIQNSLVLKSGTGSVGAELLSRNHRLPLQTSSI
                     NGRAAIEKHSIVVADTAASLTFKPNPLLPDTRSEMAIPLMVGERVVGVLDLQSSEPNT
                     LNTEILTAFEALAGQLAIAIQNASSLAETQQARAEVEAQARRLARANWVEYLDAVHVP
                     ESFGYVFDGQEVAPLDASETTPTAEGGAAFAAPIAVTGEVLGNLVVELAGQPPMARTE
                     ELVNSVARQVAQQIENLRLLESAERARAEAEKVSRRMTGEGWKEYLDVTGRDSLGFLY
                     NLSEVRPVAFDNDQLAQETGVTLPLKVRDEPVGTLMVQGLDTADNDALGLINTVAERL
                     GAHIESLRQQAQTKAALEQTENLSAAGLRLAQASDLQDMLQIIYDTLNIPVINRQLLG
                     VFNYNAANELVDMEIAANWYSGVGTEPTEIGHRYTLEEINAQAYFTIPEPLFSNDMQN
                     DPRVIEGPRQLVIHQNIRAMASMPLFIAGRQVGVLLLEGQEPYKFSPADTRLFIAMAP
                     QIATVLENRRQFERAQKQAERQSTLNLISQKIQSATTVEAVLQIAARELGHALGAPRT
                     IAQLSIKDQE"
     gene            complement(7601..9721)
                     /locus_tag="PKL11_00365"
     CDS             complement(7601..9721)
                     /locus_tag="PKL11_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNZ12076.1"
                     /translation="MGLTTTSNSNQTASQPRRTFSFRARLILASMVIIFLAVAGMGFY
                     VFYRAQQTDLYLTQQLDTTVRQQAETELQKSIAAETETLNNFFAALRKEITTLGDTTV
                     KLLDNESKLNAGDYWNAEDVLYHLSNSNWDNPNSDVVSVFIPGTVELTPDLVSQLNTL
                     VQLDFTTPAILAANPDAVAAYFGGTPGETMYYPNVDLAALVPPEFDVTQRPWFIAASP
                     AQNPTKSAVWSDPYLDAATNGLIITVSYPVYDSSGEFRGVQGMDVQLNQITAVVGNLK
                     VGQTGHAFLLDKSKRLIAMPAAAYADFGITPDAYPLGNVLDQAVLSKLSPDFAAAVEK
                     LTTGESGLETVTINGVENFIIYAPVSEVGYGLAIVVPTEELLAGATEAQEQIAQSTRN
                     SLVLGAVLVLVILILAFLAAFFIANRLVKPLGALTTVAGEIARGNLDVEAQVGGQDEI
                     GLLASTFNSMAAQLRASIGALEQRVAARTQDLETVAEVSTATATILETDRLLQSVVDL
                     TKERFKLYHSHIYLLDETGQNLVLTAGAGEPGRVMVAEKRTIPLDREQSLVARAARES
                     KGVIVNDVTQAPDFLPNPLLPDTRSELAVPMIVGGKVIGVFDIQSDQVDRFTESDVNV
                     QTTLAAQIATSIQNVRSFEQTKAQADFEALASDIGEKIQRTSSVQEALQTAIRELGVV
                     LSASRVSASIAPVRQTDADQIEPN"
     gene            complement(9754..11754)
                     /locus_tag="PKL11_00370"
     CDS             complement(9754..11754)
                     /locus_tag="PKL11_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12077.1"
                     /translation="MLNRLFDFARQGTVRIRLIVAFLLVLVLAGLVSITILGNFNSVV
                     NRLERFTSVDAQVERLLLTTSRRVASSQLNLNRYIQDYVPSPYEALDDVDQALIGLNE
                     GLALDIDKATADNLTLIITSLTNYQTQIGELQTAQDAGDAAQATRLESQLQRLGNDIS
                     VRLESMADENVKQVTATNTDVLAQAQNSIRTGVILIIVGLVMAVLLAVFNTISITRPL
                     EALRLGTEEFQRGNLEAEVSTAGRDEFTVLARTFNNLAKQIRELILDLEKRVADRTKA
                     LATSTEVSRRLSTILEQKELVAAVVDQVNEAFGYYHTQIYFFDDRQENLVMAGGTGTA
                     GEKLLAQFHKIRAGRGLVGRAAETNEPVLVPDTSQNPDWLPNPFLPETKSEIALPITL
                     GNRVLGVLDVQQNVANGLQQADIDALQSIANQIAVAIQNIRQYESTQKIASDMSVVAN
                     VGIATSTIIDPDRLLQEVVDLSKQSFNLYHAHIYLLNDAGDTLVLTAGAGEVGRQMVA
                     ENRTIPLDREQSLVARAARSREGVVVNDVTLAPDFLPNPLLPDTRSEMAVPMLVANKV
                     VGVLDVQSDEANHFTDVDVSIQTTLASQVGVALQNARSFEQIQNQAVRESAVNLITQR
                     IQSTTSVEDALQIAVRELGHALGMKPTSVILDPDALTAESGK"
     gene            complement(11765..12790)
                     /locus_tag="PKL11_00375"
     CDS             complement(11765..12790)
                     /locus_tag="PKL11_00375"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12078.1"
                     /translation="MNKRWFSLLVLALMVITPLLSACNNNGGAPEDQVTTPTAVTLNL
                     QWLPQAQFAGYYVALDKGWYAEEGIDLTIRPGGPDISALDAVRTGSAQFGTSLLADII
                     VQGQQSQDLVSIAQIQQSNGLLLIAKKDSGIATPADFANHTVGVWLGNWEAQFDALLA
                     KEGIDPNSFTLVSQGFSMDSFINGELDVASAMVYNEYYVVLESGYKPEDINIIDYADY
                     GLDFPGDTLFTSKKMIEDNPDLCLRMVRASLRGWQYAIENPQEAVDIVMKYDESGSLT
                     REHQVSMMNEIIKIVQSDLIQVGYTDRNDVRRTIDTLYSYNVLDTQVNPDLFFNNTFW
                     EQAQTKP"
     gene            complement(12859..13320)
                     /locus_tag="PKL11_00380"
     CDS             complement(12859..13320)
                     /locus_tag="PKL11_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12079.1"
                     /translation="MKTQKFPADVHARVLVVDDHPTTATTLARAISLLGPGIEAVIAS
                     TGEQAFEMVKEHPVDVLITDMIMPGINGLQLVEKLRALPEICPAYIIVITAYEIPDLE
                     KAAQKLSVNDVLLKPVRPDWICQLIASAIAHLTGQAGARPSAPPQNKHTSG"
     gene            complement(13439..14239)
                     /locus_tag="PKL11_00385"
     CDS             complement(13439..14239)
                     /locus_tag="PKL11_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ12080.1"
                     /translation="MNITGKLLIVDDEQVIRHTLARILQSSGFEVTTAENAEQGLAFL
                     ETSHFDLVFMDLRLPGMPGLDALDIIRTRCPGLPVVLFTALPDLDSAVEALRRGATDY
                     LLKPLKPEIVIERIQAILLAGQKERRKREIQAQIDALQGELNQLNDEGAALPPTASPP
                     PTSERYLKRGDIALDLYTRRVTVGENSISLLPTSFDFLLVLARHAPEVVDYQTLVAEA
                     QGYKADAREAQELVKWHIHQIRQAIEPDAREPRYIFNVRGVGYRLVSD"
     gene            complement(14236..>16222)
                     /locus_tag="PKL11_00390"
     CDS             complement(14236..>16222)
                     /locus_tag="PKL11_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12081.1"
                     /translation="DQLIGKNALDIIPFTTEARQCVLSHLHTGAIETYETEMNTPDGS
                     VISAEMSCRDIDYQGQPVRLLTLRNITQRKQTEVNLTRQLERLNVLHTIEHTIASVTD
                     LRAILNIFVQQIVDQVHVDACAILLYNPPTRTLDFAAKQGFITHALEFTHLNIGSGLA
                     GRAAQDRQVVFMPDLAEMTNNPALTQAIANEHFTAYLGVPLVAKDNLLGVLEIFHRSP
                     LTVEQDWVKFLEILANEAAIAIDNARLLSDAQQSYKETSALYQINKKLITSIDPQELM
                     DEVVSLLKVSFGYHYVQIFVADPKTGDIVMRAGSGEIGARLKAEGYHLSPGDGIVGFT
                     AETGGPFFTNSVEDLMVFVRPPYLADTRSELAVPIRSGDKFLGLLDIHQVAPMILTER
                     DVQLVTAVADQLAEALQKASLYADLQEALRHEKATRDQLVHNEKLIVAGRLLASVSHE
                     LNNPIQAIQNALFLLKEEQTLSDQGRQDLDIVLSETDRMATMLTRLRHTYRRVNAAEF
                     KPVNINDIVQEVGALVATHLRHHQISFELQLDHALPPISGLEDQLRQVALNLVMNAVE
                     AMPGGGHLKVTAQHLPDTHEALVTVSDTGPGIQDDILPNIFEAFTTNKEHGTGLGLTI
                     SQEIILRHGGRIQAENNPQRGATISIWLPVTEVKGEE"
     assembly_gap    16223..16322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16323..16574)
                     /locus_tag="PKL11_00395"
     CDS             complement(<16323..16574)
                     /locus_tag="PKL11_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ12082.1"
                     /translation="MDDSRPRLQQESGDMSGTPEGGILKTNEHRYQHLASLTTEGLMI
                     HRDGVILDANLAFAEMLGYPNPDQLIGKNALDIIPFTTEA"
     gene            complement(16742..17632)
                     /locus_tag="PKL11_00400"
     CDS             complement(16742..17632)
                     /locus_tag="PKL11_00400"
                     /inference="COORDINATES: protein motif:HMM:NF023982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12083.1"
                     /translation="MTLPPSPAIELVPASQFTIEQLTALYNQTRVDYLVPMPMNAARL
                     AEYVNTYDVDLDHSLVARQGDDILGLAMLGVRKDRTWITRLGVLPTTRRHGTGQALME
                     GLLEQTHRLGIKFCMLEVIKNNTPAHTLFLKMGFKEIGELLVLRRSPTLPPPDPVIAD
                     AQRLELVEALDLVARNRGTQPWTNQSETLNNARDVSGLRVTLPDGSHGWLVYQRQKFT
                     LTRFAYKTDVGDPTAVATAFLSHLHYMYPRLDTQLENIQVNDPHLPALQKTGYVEAFR
                     RIEMWWGDPPPSLLASKAKS"
     gene            complement(17604..18008)
                     /locus_tag="PKL11_00405"
     CDS             complement(17604..18008)
                     /locus_tag="PKL11_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12084.1"
                     /translation="MTKILLIEDIPDNAELTCKILVSAGYEVVHAPEAELGMEQAVSV
                     KPDLILLDLGLPDYDGQTLAGWLSADPRTAAIPIVAYTAWPEATARQMVTSYGCAGYI
                     PKPIIKVNDFLSQIASFLKPNQSYDSSTQPGN"
     gene            complement(18181..18711)
                     /locus_tag="PKL11_00410"
     CDS             complement(18181..18711)
                     /locus_tag="PKL11_00410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ12085.1"
                     /translation="MKFEVLHRDVVYQGRAFDVARLRLRVPGGDQKHYDLVEHRDSIS
                     IIPLDSDGQIWFVRQYRVGVDGELLELPAGVLEPGERPESGAAREVREEVGMAAAELR
                     YLGGFYLTPGYCNEFHSIFLATGLYSAPLAADPDEYLQIEKLPVAQAFQRAERGEFRD
                     AKTMAALFLLKGALPG"
     gene            complement(18708..19322)
                     /locus_tag="PKL11_00415"
     CDS             complement(18708..19322)
                     /locus_tag="PKL11_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ12086.1"
                     /translation="MTRKFLIPVLAIVILALALSSCKLPASTPAPVAATATSEISFPT
                     SPAAPGTTPVVQSQPTAQPTPTVMVVTAITPIPPVSPTANPYTYVAPARPQNYTIQKD
                     ESPYCIARRFDLDPDSLLALNGLTKNSIVQPGDVLKIPQTGNWSAGNRYLAAHPATYT
                     VRSGDSIYKIACAVYGDVDPLAIAAANGLAAPYTLSVGQVLQIP"
     gene            19529..20251
                     /locus_tag="PKL11_00420"
     CDS             19529..20251
                     /locus_tag="PKL11_00420"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ12087.1"
                     /translation="MTNTPSPTLTSLDNPISLEMRAYDALKAAILSFALMPGEPLVEA
                     ELARRLGISKTPVRNALARLEKEGFIAKQPYKGYNVAEISQQTVIEIFAVRAALEGLA
                     AREATAHLTSEALAEAAVLLAEHAGAAERGDLEQASTLNRQFHDLLLRLANNAWLTQI
                     LSNLDEHLQRYRLLSAFQAGRLEKSVAEHAAILQALQAGDAAAAEVAARQHLFSVSAD
                     LAEQDFNQLVGLAHQQRSPAVE"
     gene            20294..21763
                     /locus_tag="PKL11_00425"
     CDS             20294..21763
                     /locus_tag="PKL11_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12088.1"
                     /translation="MSPKFNYRYQLYKHLQANTQAIYAESRKAAEDLGLLPEFRGALG
                     LTGAVSGCHGLLSRAVEEAVNEVSRKVLPSAVLDEQLRDLVKSYYGDEYDAALVNTCE
                     AALNTAFDVLCMPPTLGRGEPYRTRYMALYERHLHHQGAYGRPFPPKYKEINAERGEA
                     AGEYGVQGKRAFNLDTLVVKPVGATYECHGIKFNPIPNMLHVDAAATIERVAEACARQ
                     PELMAGIASLGYDTPGYGYGQKDANGAPLLQVGLAEIANCYDIPYIVDNAWGVPFIGT
                     DIRKTGAHVMMYSMDKASGAPTCGLIIGKEEEMVLMRRALGIHGARYGTLSSHGKASH
                     VGFDPGKEALAGAIAAMRILKDQPEITLKALDDLYTIVMEEFESLPTPLRQGWVIYKS
                     VNCQSVELNYYDSWANGAWGLPVFSIEDMYAGSHLLQNAMSQMGMIPTIAYDANIMIS
                     NGVGNLDEEGRLLERPTRLAVRGMFKIMEILGRYAGMLD"
     gene            21905..22321
                     /gene="ssb"
                     /locus_tag="PKL11_00430"
     CDS             21905..22321
                     /gene="ssb"
                     /locus_tag="PKL11_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ12089.1"
                     /translation="MSFHTIVVVGRLGRDPEMRYSPSGDAVTSFSIATDRQYTDSSGQ
                     RVKETIWFRVSVWGKQAESCNTYLKKGKMVLVEGRLVCDGATGGPKIFKRQDGSSGAS
                     FEISASTVRFLSPRTEGEFEPGEAPAPEAGDEDIPF"
     gene            22328..22651
                     /locus_tag="PKL11_00435"
     CDS             22328..22651
                     /locus_tag="PKL11_00435"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNZ12090.1"
                     /translation="MTHPTPPPGLELPPDLEPLYSNLARISHTPAEFVIDFARLLPGQ
                     TNLNVSTRLVMSPVSLKLFFRALAENLARYEASFGEIKLPGDTSLATDLFRNIQPPPP
                     PPAAE"
     gene            22658..23311
                     /locus_tag="PKL11_00440"
     CDS             22658..23311
                     /locus_tag="PKL11_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HNZ12091.1"
                     /translation="MEPLQILSEQVHQIFESRNQARDRALAQARQLTRHAAQSIRAIH
                     RSEVETAHQHLTEAAAIVAALRQELAAFPDLFYAGYTQDAIKEFAEANITCALIENRA
                     LPMPEDLGVEYATYLNGMAEVPGELRRRCLDILRSGYSAEAERLLGWMDEIYSLMITV
                     DYPDAITNGLRRQTDLLRGIVERTRADLTLSQREDRLHNAMSSLAEQLADHAGDSSL"
     gene            23289..23528
                     /locus_tag="PKL11_00445"
     CDS             23289..23528
                     /locus_tag="PKL11_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12092.1"
                     /translation="MRAIRASELGSYRFCERAWWYQLQGVESDNLTDLAAGSSYHQSH
                     GRRTLSAGLMRAAGWLLLLGGLVTLVVWLTSQAIK"
     gene            23535..24044
                     /locus_tag="PKL11_00450"
     CDS             23535..24044
                     /locus_tag="PKL11_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HNZ12093.1"
                     /translation="MPLLIILLLVGAVLLLWLGGRQRKESGLPTGRIIYADPALWGET
                     EAPLYDSSTGLTGRPDYLVRQGEETLPVEVKSRYAPAEPYAGHVLQLAAYCRLVEAET
                     GRRPPYGILVYRNRSFAIDYTPALEAELLDLLKEVRQQEMRGEPDRSHEEPGRCERCG
                     FRKTCNQRL"
     gene            24101..24937
                     /locus_tag="PKL11_00455"
     CDS             24101..24937
                     /locus_tag="PKL11_00455"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNZ12094.1"
                     /translation="MMHASLPEAAGEAEQICQFLKLRGQTCVCASFSDEAMRQQVQAG
                     EFDLVIALGGDGTMLRASRLCALPGTPVLGINLGHFGFLTEVDRAEWPTRLAQLLEGN
                     YRLERRMMLRAEHWRGEEMLAHWLLLNEVVVCRGATVRPIRVSAWVDNEPLTSYVADG
                     LIAASATGSTAYALAAGGPVLPPELRSIVIVPVAPHLSMDRAIVLPEGAQVVFQVHGH
                     SPQAVYSGDGQTPVPLQDGDAIHTRADAHTAQFIRFQAPGIFYRNLTMYMEQNPTAGL
                     PQ"
     gene            24934..25476
                     /locus_tag="PKL11_00460"
     CDS             24934..25476
                     /locus_tag="PKL11_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HNZ12095.1"
                     /translation="MTQETTDNSYPTLYCANHPQRPTLLRCNRCEKPICSDCAVKTPV
                     GYRCKECVRGQQKVYDTAKKWDYPVAFLVAAIIAFLGSFTTAIGFFTIFLTPIIGVLA
                     AEAVRWATRRRRSPALHKIAALSAGLASLPLVIFQVINTVLMLSDGYGLWYAIGPMMW
                     QGIYLILIISTVYYRLSGKG"
     gene            25489..27240
                     /gene="recN"
                     /locus_tag="PKL11_00465"
     CDS             25489..27240
                     /gene="recN"
                     /locus_tag="PKL11_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNZ12096.1"
                     /translation="MLTELRIENFAIIQHLELSFGSGLIAFTGETGAGKSIILDAVEA
                     VIGGRADAAQIRSGAERASVEAIFRLPETSRDDIAALLEAEDLLDDPNEIILGRELRA
                     NGRSIARVNGRTVNLSLLRSLGSYLVDIHGQSEHLSLLNVRAHMGLLDRFAEASEALA
                     VYRQTYQAVRAVQRELVGLQTQEADVARRVEMLTYQAEEIESARLHAGEEDELRLERN
                     RLANAENLASAAQQALTLLDEGSPEAAAITDLLGQMVQLLHTLERVDPTQAGLAEQAA
                     GLSENAADLALSLRAYLEQIEANPRRLEALEDRLALIHDLKRKYGGSIEGALARAVEA
                     RQQLEAITHSAERIAELQSQMTDLRARLTAQAQTLSALRQQAAVKLGQAVEVELADLN
                     MGGARFAVDLQTEPDANGIEVIPGKPVSFDENGVDRCEFLIAPNPGEGLKPLVKIASG
                     GETSRLMLALKKVLTEADAVPTLIFDEIDQGIGGRVGMVVGEKLWLLGRRHQVLCVTH
                     LPQLAAYGDQHYCVRKQVADGRTLTQVEALNDAGRRDELAQMLGSLTAANRSAAQETL
                     AVARQRALELARRAQGS"
     gene            27244..30768
                     /locus_tag="PKL11_00470"
     CDS             27244..30768
                     /locus_tag="PKL11_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ12097.1"
                     /translation="MAPDGLLQILMLGPPAVRQNDQLIEIPRKQTRGLLFYLACQPGP
                     VTRDELVDLFFEDEDEMSARRHLTEAITKLRGALFAPIYVVTKPETVELDARLIYSDV
                     VEFSRLIHESGRPGGSGNRPQLPTATFQRLSRAVDLWRQPARFLSGVRLPEGNNLEKW
                     LKQLSQELEVDWMTAITQLADHLAANGELQTAIERLQLGLNLDPTNAGLNIRKLRCLI
                     QSGNIKQAAEYANFVAEQYDDEGLYLPGDLAEVMQQVLTLENQPVPELHPNWEGLVQA
                     QNKFVGQEEALEELKRAYQRGGAALVLGEAGSGKSRLVYEFVRRQSPQPRQLLALCRP
                     LEENLPLQPWVDLLRRHVQPSEWQSIEAAWLKPLARLLPDLAILRPELKPEQDADNEV
                     PQTQFFEALHQVLLNLASRQRVVLFVDGAQWGDEATYAALVYLLERDFFNKNGLLILA
                     VRPQESNPSLEKLRNASNAGRSLTAIDMQPLNLDEIAQVARSVLGQAPTQQLAFRLKM
                     DSGGNPYYLLELLYALQDLHVDMNNPYLPEALPSSGSLIALIQTRTQQLPPQCREVLE
                     AAAVIGKRFTLDHLQAVARLPMETTAETLTMLIRAQLIREMPEARPAGGYGFVHDITQ
                     EAIRQSLLRPRRQVLHRRLALALEASAQPQTINLEKLAYHWEEAGDLQKAFNYWFENG
                     KNAEINARWQEAKNAFKRADRLREQLGDALADQQIYLLYRWWAGTFFYTMEIDQLRLT
                     SQKLFQAGEQRSSALMMGCALRCMADADAMEHQPAQGLVNIERAMTYLEPIQDYYELV
                     EAQSCRGLCLWQLGRYSESLDAWTAAAALSENQEPIGTRAAGLFARIRLAIAFGRLGW
                     PRKAEAEARKIIQESLELRRPVLGFYGRNILAGAQDRMGQHYMALEITRSVLAAARES
                     HNPGLVGNILFVMAATQTQLGRLDDAWETITTLLNNVSQIEFVDILKLANLLRGDIFR
                     LLEFYPAAVKNYQDGLGSTLSGIYTLENLLGLGTTLLLQGEGDLGHHFLERVINEAQA
                     QELNIYQIEAELALTYAQILNGEIETAAQRLEPLERQLAERELTVLPCNPDKLHGLMA
                     LQRGDYPAALALGQACLNRGLERQNPWLQMEGLELCLAAAPKEEAASWQRQIEALLNW
                     LDSQTRHAELRPAFANFQKRVLERISRR"
     gene            30858..32150
                     /locus_tag="PKL11_00475"
     CDS             30858..32150
                     /locus_tag="PKL11_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNZ12098.1"
                     /translation="MNTKPSTTNRGCVVGVVLLFSMLIWSVLPIDVRAQQSQTSITTY
                     LPLILKPSGGPQYVLVAWNDLGMHCYNRDFSSLAVLPPYNNLWAQVIRRGDPPQIVSD
                     NIQVTYLFPDNTYSVEKSNFWDYAQDLFNLGTPLTPNIGLTGVGMAGEMAFNPTNNAF
                     IVEGIPLTEFRDSDPSTPYPYQLAEIAAKNATTGTALASLTVVAPVSTEMTCNDCHSD
                     NGRGNEGISTGDVEINILTKHDDENSEDYPVGGEYGNSLVGNQPVLCADCHSSNALGL
                     PGAPGLPSLSNAMHTKHAEIDPPQPNCYQCHPGPTTQCLRDVMSTQYGMTCEDCHGAL
                     DVVAQNPDPWLQEPRCDGCHTGSGYEQNNALYRHSTGHGGVYCEACHDSTHAIAPSNQ
                     GNDGIKFTQLQNAAGPLTKCTVCHLTDPGGTNPHDVIP"
     gene            complement(32163..33206)
                     /locus_tag="PKL11_00480"
     CDS             complement(32163..33206)
                     /locus_tag="PKL11_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNZ12099.1"
                     /translation="MIPPINSEQMLAFLAGLLNTPSPTGFADAGINFTEAALQAYPAL
                     TFTRTRKGALVARWPGQADDSPRCLTAHVDTLGAMVKEIKSNGRLKLTKIGGYAWNTV
                     EGEGCTIFTADGRKVRGSLLLAKASTHVYGAEVNDAKRGDDAMEVRLDEITSSADQTR
                     ALGIQVGDFVAFDPRVEITNGFVRSRHLDDKACVACIVAAVAAISAAGLTPRQTTYLH
                     ISNYEEVGHGASAGVPAEVTELISVDMAAVGEGQTSDEFHASICVKDSSGPYHHGLSN
                     HMRRLADQYQIPYKADIYPYYSSDGAAFWRSGGDVAVALIGPGVDASHNYERTHTEAL
                     LATTNWLLAYLLN"
     gene            complement(33240..33668)
                     /locus_tag="PKL11_00485"
     CDS             complement(33240..33668)
                     /locus_tag="PKL11_00485"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNZ12100.1"
                     /translation="MTQFNFSYPIPIRYSDLDPQWHVNNARFLSFIEQARYAYLIELG
                     LWDGVDFFNLGLIVADIHMAYLAPIELGQTVRVYIRADRLGNKSLTFAYEIRDEATGL
                     LHARAETVMVAYDYHTHQSIPLPQSWRERITAYEAAAPAN"
     gene            33744..33818
                     /locus_tag="PKL11_00490"
     tRNA            33744..33818
                     /locus_tag="PKL11_00490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33778..33780,aa:Val,seq:cac)
     gene            33926..34687
                     /locus_tag="PKL11_00495"
     CDS             33926..34687
                     /locus_tag="PKL11_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12101.1"
                     /translation="MPTESPTPLIELRQVVKVYQTAAGEFPALRGIDASIYAGEFLGI
                     IGKSGAGKSTLLNMLTGVDHLTAGEVVVWGNGEPVAIHKLSENDRALWRGRTMGVVYQ
                     SFQLLPMLTLVDNILLPMDLCGLYKPGASRARAYELLRMVELEEHATKLPDFISGGQQ
                     QRVAIARALANDPPILVADEPTGSLDSVTADHVFQVFEQLVSQGKTIIMATHDASLMP
                     RFSRHLYIQDGQIVPDPTPADPPATLMAAPHRRKR"
     gene            34684..35811
                     /locus_tag="PKL11_00500"
     CDS             34684..35811
                     /locus_tag="PKL11_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ12102.1"
                     /translation="MIPAQVSTQTGETAIQLQKVVKTFKNAAGEFTVLKGIDLNLHRG
                     EFVSIVGKSGSGKSTLLNMITGIDHPTGGQVIINGVNIYTLNESQRALWRGKNLGIVF
                     QFFQLLPMLTLLENVMLPMDYAGLYDFDERPKRALGLLQLVGLEGQAHKLPAAVSTGQ
                     QQSAAIARALATDPPIICADEPTGNLDSRSAEAIIGLFERLVEAGKTIAMVTHDPTLT
                     RRTTRTIIISDGELIDETVAQALPQLGHRQMLEASRQLERFTCQPNSPILQQGQHVDY
                     FYMIVSGSVEVVLCNRRQEYTVARLGPGQFFGEVELIHGGQSIACVRAVPDGPVTLAA
                     LSRDKFNQLIKSSPLTEEALAQVVQLRLAENRTGDRRQRQR"
     gene            35829..38261
                     /locus_tag="PKL11_00505"
     CDS             35829..38261
                     /locus_tag="PKL11_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12103.1"
                     /translation="MRPRWRKVLHDLIDNISRTTLVVLSIVVGVFSIGVIIGAYVIIS
                     NDMSASYAANHPMNIELRCGAFDDDLLSMVKHMNGVEAAEGRSFFTMRVRLAGGEQWE
                     TLNIVGVDDFEKLDINRLNIISGTNLPADEQVLLEKKTLDNLDVVVGDTLEFELLDGS
                     IKTLQVVGIVQDPATGAGDFLAQPFAYVTTGTLPFLGQTESFNRMFVTVTEGQNDLEH
                     LDEMSAAIRDKIEKSGIGVYTSRTTKTNEHPMASTVQAVLGILLALGVLILFLSSSLI
                     ANTLSALLNQHLRHIGVMKLVGARRGQILQMYFALILAFGVIALVIAVPLGGQGAYSL
                     ANFIASKINFNLLGYRIVPFSLLVQIVVGLAVPLLAGLLPVLSGSRTTVLQALTGSIN
                     EDSHSQVAGSLPEEAGFERLEARVAHTLGRQSIHVPRPLLLSLRNTFRRKGRLALTLF
                     TLTMGGAIFIAVFNVQVTLHDYIDQIGKYFLADVTLDFDRYYRLNDVKTTAMNIPGVV
                     DVEGWAYASAEVLYPNDRVARNITILAPPASSQLVSPLLVEGRWIQPGDEKVITISEA
                     ILEEFPDLHAGQTLRLNVNGRKDDWLVVGIFKFVDQQGTFAYSTYEYISHQAHMANRA
                     FSYRIVTVQHDPAYQQKMGEILDAAFRARGFHVSQARTGQSTLALAAESLDILVTFLL
                     IMALLTALVGSIGLAGTMGMNVMERTREIGVMRSIGAADKEIMRSVIIEGMVIGMISW
                     LLGALLSFPFTYLLTYIISVAIFHSPIAVHFTATGFGVWLLVVLVLSAVASALPARNA
                     ARLTIREVLAYE"
     gene            38285..39445
                     /locus_tag="PKL11_00510"
     CDS             38285..39445
                     /locus_tag="PKL11_00510"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNZ12104.1"
                     /translation="MKKVPMIVILILVMLLTACGKATEAAPIANPAPAVDVVIAEGHL
                     IPNQSQYLVFPVRGRVAEILVSEGDSVDMGQVLIRLGDSEQAQAGLAAAQLELTQAQQ
                     DYDALIRTADLGHAQAWQAYIDAQKVRAAAQLAWDRLDLSAIEIEIDNAQAEVTSRQT
                     DLDEAQTDFDKYRFLPVENVTRQTYEDSLRTAQIDYDTAVQKREDLAHQRDAVQATLD
                     AARATEAEALRDYDNTVSGADVDQLALAQARLDAAQAQVSAAQAALDNYELKAPFNGT
                     IADIDIRVGEWAGPEAWVAALVDTSAWYVETSDLSELDMVEIGVGQSVEVTADALPDV
                     VMTGVVESISAAPRIQGSDILYTVQIRMDEVDPSLRWGMTMEVTFTPQEANR"
     gene            39531..40289
                     /locus_tag="PKL11_00515"
     CDS             39531..40289
                     /locus_tag="PKL11_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ12105.1"
                     /translation="MTEFSRMPRYDIRNDGAGPYAIFYCDICGREFRSQPDVVGTISS
                     DIGRQAASGLLRKIPLVGSAVANKVTGEDPRYSYNLTPAQLESAWKQVQQHFRQCPTC
                     TRIVCLSDFDTQSGYCQEDSPRTDEIAESRGQQAGAALKGFAAALGLDRVASQVSNAA
                     EAAQRATSAMARCPKDGTLAAPGTKFCPECGTAMVQPAAAACPKCGADVHGAKFCPEC
                     GTKIEAAPRPANCPQCGTATEGAKFCPNCGAKQG"
     gene            40403..40864
                     /locus_tag="PKL11_00520"
     CDS             40403..40864
                     /locus_tag="PKL11_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12106.1"
                     /translation="MTPIVRPCELKDLPAVLGLLTQLEEVAHAQSDLKLERLRELFVE
                     MAECPEIYLNLVCEADGQVAGFLSMIFYRTFFHRGGTALVNELVVDEKRRGLGLGQLL
                     MQTAITEALARDMDEIEVGTEEDNQAAQAFYSRAGLNERYVLLGMEFETEE"
     gene            complement(40865..41902)
                     /locus_tag="PKL11_00525"
     CDS             complement(40865..41902)
                     /locus_tag="PKL11_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ12107.1"
                     /translation="MSLYAEIFEQPAVLTRLLTTQRDAVLAIARDLRQRSVDYVFLAA
                     RGTSDNAGRYANYLWGAANQLPVALATPSLFTFYHQPPRLKNALVLGISQSGQSPDIV
                     GVLEEGRRQGCPTLAITNCADSPLAKAADYVLDICAGPERAVAATKTYTAELLALAML
                     SAALADDAAAWEALAQVPDWVAQTLQQDSSIATAAERYRYMQQCVVLGRGFNYATAFE
                     WALKLKELTYVSAEPYSSADFLHGPIAMVERGFPVMAVAPTGQVYAPQMELLHHLKDD
                     LLAELVIISNDESALAMGKSAIRIPAATPESLTPIVSIPAGQLFAYHLTRAKGYDTEQ
                     PRTIRKVTETR"
     gene            complement(41930..42781)
                     /locus_tag="PKL11_00530"
     CDS             complement(41930..42781)
                     /locus_tag="PKL11_00530"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ12108.1"
                     /translation="MKFHLDTADLYIPDGQPAAAALARTTHLAIAAHQDDIEIMAAAP
                     ILECFQRTDRWFTGVVVTDGRGSPRAGLYAQYDNEEMRLVRFKEQRKAACVGEYAAQV
                     ILDYPSSAVKSGADDRPVEDLVALLKAARPQVVYTHNLADKHDTHVGVALKAIAALRR
                     LPPAERPQQVFGCEVWRGLDWMLDEEKIAFDLSDQENLQSALLGVFDSQISGGKRYDL
                     STLARRRANATYYASHGVDTATGLAFAMDLTPLVADPDLDPADFIRAAIDRFQSDVAA
                     RIARLRA"
     gene            complement(42791..44182)
                     /locus_tag="PKL11_00535"
     CDS             complement(42791..44182)
                     /locus_tag="PKL11_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ12109.1"
                     /translation="MASLTLVPPAILPPLDPNFRPASLANRHYRRRVGAAGVPVVLGL
                     EREDGRISRCETAIFPPGHPEAGENLPYIERLVKFLLWQRGGFRLYVGGPAELAAQLR
                     AIYALTGPRRFDVDFMGSQVYQHPFEVVACRPEDVPPAREQGQAIGRHLNGCRIGFDL
                     GASDRKVSAVLDGRVVFSEEVIWEPKNHADPAYHYREILTAIRSAAAHLPRVDAIGGS
                     SAGIYIDNRPMVASLFRAVPPDQYDQIRTLFVRLSAELGVPIYVINDGDVTALAGAMS
                     LEENGILGIAMGSSEAAGYVDVEGHILGWLNELSFAPVDYAPTAPVEEWSGDAGCGSQ
                     YFSQQCVFRLAPQAGIHIPANITDAEKLQTVQALLEADHPGAVQIWQSMGIYLGYALA
                     HYADFYTMRHVLILGRCTSGRGGEIILQTARQVLAAEFPDLAGAFQIQLPDEKSRRVG
                     QSIAAASLPEIKP"
     gene            complement(44324..45742)
                     /locus_tag="PKL11_00540"
     CDS             complement(44324..45742)
                     /locus_tag="PKL11_00540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNZ12110.1"
                     /translation="MPKSYQASLARIEQRFRTFHPFYADGWRELPLTERMAARFIPGA
                     SMAIVEGGEIIWAGAYGVRRQGDPDPVTPETCFQAASISKPVAALTALSLVAEGRLSL
                     DADLRDFLRTWQIPARAGWQPRVTLRQLLSHSGGITVDGFLGYPARSAVPTLQQILNG
                     EPPANSPAIRVDTLPGFHFRYSGGGYTIVQQLIEDLTGQPFWQAARQRVLEPAGMTHS
                     TYLDPLPADWHSRAAVGHRNNGDPLIGNWHRYPESAAAGLWTTPTDLARLIVLLEATQ
                     RGETDRLVRPDLLADMLTPQIPLRGIGLDGDEGLGFFLTGEPDDLYYGHGGSNEGYRN
                     QILARKGRGQAAIIMTNSDSGDALTDEWINNVALEFGWDSFVDTPFLPMPNPPANAAL
                     AEFAGDYASGDWQFHIRHTPAGLTLEIPGQPALALCPRDVDRFYLTQLNASIQFQRED
                     GRITALAFRQNGEDLTAERVAE"
     gene            45977..47278
                     /locus_tag="PKL11_00545"
     CDS             45977..47278
                     /locus_tag="PKL11_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12111.1"
                     /translation="MSTTPPAVVPFESQPPQVMKRNFFLGAINGVLFVFAETLFDPTL
                     VVTSLANQLGASAFILGLFGPISSAGWYLPPFFVAGYVQSRPLKIAVYRGMSFIRIAC
                     WILLAAVVNLVRDPAWILISLIVAYSIASLASGIGGLPFLEVVSKTVPPVRRGELFAW
                     RLGLGGLLGITASGLVRWLLDPATPITFPNNYGLLAIGFCLFASISLMLFNQIKETPD
                     ANPRPSRPFRQQLRIALGIVRTSPNYRRHLLTQALLFVGVSAVTFYMVYVNRELDAPL
                     QWVGVYLAVQMVANLLANLLFGWLSRRVGNQKVLTVGAAAGVIAAAIMTGMVLLAKPL
                     AISGLTAAWLLTPVFAMVGIRVAAVGVASPSLMLNIVPPEERSLMIGFTQTVGGMVVL
                     LTMTSGLIVGAVGYPALMIISLAAYIGAVLLALQIREHPDL"
     gene            47337..48239
                     /locus_tag="PKL11_00550"
     CDS             47337..48239
                     /locus_tag="PKL11_00550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNZ12112.1"
                     /translation="MNTTAQTSRKYLWIYILIAFGFTWALWIPTLLLSEQKSYTLPLA
                     ANYPALFEEGFVNSEHILLAVLFSLAVYGPLLAATITAGLEGGKSGVQALFASFKRWG
                     SGKWYGRAALVLIAVAGVPMLIGLLTGLTKPNGTALLAFLPWLLPIFIMQILTSGLGE
                     EPGWRGYLLPRLQQTLSGEKPVWVLGIIWAVWHFPFTIYSTLATTVEMPAAALVTTII
                     MSLAGQTISLIGVTYLYVWIYNHTRSLPLVIFFHALSNTLPLIPLGGWSPLVSLLTAA
                     MPWVIVFVLEKKLPKGEFPGQPVQ"
     gene            complement(48243..49019)
                     /gene="recO"
                     /locus_tag="PKL11_00555"
     CDS             complement(48243..49019)
                     /gene="recO"
                     /locus_tag="PKL11_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNZ12113.1"
                     /translation="MPKEQRSLRVEAVVLRHLDWGEADRLLTLFTREAGKLRAVAKGA
                     RKVRSRKAGHLEPFTRVALMLARGRDAWIVTQAETVDAYLPIGQDLTRIGYASYVIEL
                     LDRFTYEEGENRPLYNLLTDTLARIAALPDPFLAVRYYDIRLLDLSGFRPELFHCVGC
                     GALIQPQDQFFSALQGGALCPRCGLNPGAQAWPISMAALKYLRHLQRSTYAEAARADP
                     SEPVRREMESLLQQYLTYLLERRLNTPQFLREVRRTYPAE"
     gene            49090..49725
                     /locus_tag="PKL11_00560"
     CDS             49090..49725
                     /locus_tag="PKL11_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNZ12114.1"
                     /translation="MYLQPEDLLKIVLAVLVGGLIGIEREYRDKAAGFRTLIFICVGA
                     TLFTILSVRLAGDNDPTRIAASIVTGVGFLGAGVILRDGGRVIGLTTAAAIWLTAALG
                     VGLGGGLYALVGVSTVLALIVLWVLPFFEHRLDNLWEERTYEIVCALAPEKVAELEHL
                     FAQNKLRVKTHRLTRSGGEMTLNLLVRGPLKGHEKVLEQIFARDDVHTCHY"
     gene            49740..51200
                     /locus_tag="PKL11_00565"
     CDS             49740..51200
                     /locus_tag="PKL11_00565"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNZ12115.1"
                     /translation="MIDWIIIGAAGLLVVSVLASRISDRFGVPVLLIFLALGMLSGSD
                     GIGGIYFDNPQIAQSVGVVALVLILFSGGLDTEWRSVRPIVKEGLLLSTVGVLLTALG
                     VGLIAALLLKLSIVEGLLLGAVISSTDAAAVFSVLRAKGINLKGQLKPLLEFESGSND
                     PMAIFLTVGLIQILTQPDFSTLDLLGSFVLQMLLGAGLGVAAGYGMLFLLNRIRLGYD
                     GLYPVFTTALVFLTFGLTNVVGGNGFLAVYLAGIMLRRGDFIHRKSVMRFHDGLAWLM
                     QITMFLTLGLLVFPSRLPPIMGVGLLIAIGLMFVARPIGVFLALLPSKLKIREKAFIS
                     WVGLRGAVPIILATYPLLAGLEQAELMFNVVFFVVLTSTLFQGTLIPQVARWLKVDAP
                     LEAKRVYPLEYTRMEGLSSELKEYPIPADSFVVGKSIVELGLPQQFLIMLIARGNEFV
                     LPSGGTVLEAGDVLLALTDAESSEIVREQIAIKTAS"
     gene            51238..54204
                     /locus_tag="PKL11_00570"
     CDS             51238..54204
                     /locus_tag="PKL11_00570"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ12116.1"
                     /translation="MSTASVPLAFLFTDIEGSTRLWEAHPDAMQAALACHDARLRAVI
                     AAHGGEVFKTIGDAFCVTFSSPLEAVQAALEAQRELTAEPCGALELRVRMAVHSGTAE
                     SRDGDYFGQPLNRVARLLAAGHGGQVLVSQAVHEQVKDQLPPEVCLLDLGERRLKDLA
                     QPEHIYQLAGPGLRDDFPALRTLEAFRHNLPTQLTSFVGRVRELKELQNRLSEQRLVT
                     LIGPGGTGKTRLAIQAATEMLDHYADGTWLVELAPVTEPTQVMRATAMTFGLRESEDR
                     PLLQVLNDYLHGKNLLLILDNCEHVIDACASLAEALLEVNPQLHLLASSREALGLTGE
                     NIFRVPSLEAPDPRHLPPWEQFSHCEAVRLFAERAAAAQPEFELTPDNGPAVAQICQR
                     LDGIPLAIELAAARVKVLSVEQIAARLDDRFRLLTGGSRTALPRQQTLRALIDWSYDL
                     LNEAERVLFRRLAVFSGGWTLEAAETICSGSINGQELTEWEVLDLMSGLVNKSLVLTE
                     LRGGETRYRMLETIRQYTRDRLLESGESTACRNRHLQYYLNIAEQLEPELWRDQQLAA
                     LKRLDRENDNLRAALEWCLAEESGPNISAGLRIACTLPPYWGIRGLWTECNERFQTLL
                     GYPNAEVSDRLRLKALNRAGLAAREQSNSTLVQLYFGAALPLAEKLGERGEQAYALLG
                     LHRTDESIAIFRELNDPVGLLRALAARGTIANAAGDFETAKRAFEENLALCHAIGHRI
                     GAAGTLVALSFLGRMDNDLDAAERAGQEALEIFRTADDRPGIGGATYMLGLVKMQRGD
                     LDAARVYFNENMAIQNELGVVPFIGGATLILSELERLAQNYDRALELLERCQKIFVRA
                     QDPLNLALTYINQADILQRQGRMDEAREALKIYFASPGINYATYALGLLNVAYLGWQA
                     GQAETGVRFLGAFAGVPGIEQLLTPADYEQMETVTTGLRALLMPEAYQAAYAAGMQLA
                     KEDLGAVIAEAVDYLR"
     gene            54228..54620
                     /locus_tag="PKL11_00575"
     CDS             54228..54620
                     /locus_tag="PKL11_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNZ12117.1"
                     /translation="MEKPAYKYSDITQKIIGAALKVHSALGNGYLEVIYQRAMVIEMR
                     KQNLSFEREVEVPIFYDGEHIGTRRVDFIVEGKVIVEIKATTQLQDVHLAQAINYLEA
                     YGSEIGLLLNFGAKSLQIKRLIKSRGES"
     gene            complement(54982..55938)
                     /locus_tag="PKL11_00580"
     CDS             complement(54982..55938)
                     /locus_tag="PKL11_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNZ12118.1"
                     /translation="MTNPFWNPVEKRVRSLWRLVIQALFMFLVAGIISAVPVVVLTLL
                     SSQIPSNFFLMNVVTVIGMALAMLLSLWLAGLFLDRRPLLDFGLHFSRGWWLDFAFGL
                     ILGAVLMAIIFGVELAAGWVTITGYWQVDTPGLPFWATFSLILVQFICVGIYEELWSR
                     GYHLRNLAEGFNFGGKMPRAALLVAYLISSSVFGLLHMGNPNASLISTFNIILAGLFL
                     GLGFVLTGELAIPIGLHITWNFFQGAIFGFPVSGTPRTATLIGIQQGGPDLWTGGAFG
                     PEAGLIGLLAIALGSLLIWLWVRWRRGTSPLHTRLAVYTPKS"
     gene            complement(55953..57392)
                     /locus_tag="PKL11_00585"
     CDS             complement(55953..57392)
                     /locus_tag="PKL11_00585"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNZ12119.1"
                     /translation="MPRDRQVYLLDPQDLPPETIAVTFAKTSRSPQSFRQIAAELTDA
                     RSADFHEKWVVGYGHASVAEHAVLHIAVENISRLAVETLESNRLASYTEKSTRYQQWG
                     PEDFFIPPELDRHLLRERFAETCRDLFATYTRCLPAVLEAIQREDPRSPTESEEAWLR
                     RVRPQSVDVCRFLLPAAALANVGMTINARALEHAISKLLSHPLAEVQAIGAEIKFAAR
                     QSVPTLVKYADAVPYLQSTAARLSAQSAALPRALDVLAPDETAWCRLVDFDASAENRA
                     LAAALYRFGSQSYAQALAAVQALPPAGRSQLAQAALADLGAHDIPLRELEYATFTFDL
                     ILDQGAYAELKRHRMMTQTPQALTAGLGYALPRRFAAAGVAGDVRRALDAAHQTWAAL
                     AEFDPNVASYIVPNGFNRRVLLQTNLRSLMHFIALRSAPNAHFSIRRIAHRMAELVQQ
                     AAPLLGSYLRTAPGETWQQVEQAHFETTA"
     gene            complement(57382..58308)
                     /locus_tag="PKL11_00590"
     CDS             complement(57382..58308)
                     /locus_tag="PKL11_00590"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNZ12120.1"
                     /translation="MRHEDSLEALHLTGAWVTIGTFDGVHRGHQALIRTLVQDSAAAD
                     APAVVITFTPHPLVALRGSTGPYCLTSPANRARLLGELGVDVVITLPFNADLAALTAD
                     EFMQRVQRALGLRHLRVGPDFALGRGRAGNVEKLRQIGAELGYQVSLFPTVTLDGEPV
                     SSSRIRSALLAGEVGTAARLLGRPHSVEGLVVHGDGRGRGLGFPTANLDISPSRLLPA
                     NGVYTTWAWAGGERLPAVTNIGVRPTFNGEPALRVEAHLLDIDRDLYGQVLQLEFLSR
                     LRTEQRFNSIDELMAQIRRDAQSAREVFAHAA"
     gene            complement(58311..59225)
                     /gene="truB"
                     /locus_tag="PKL11_00595"
     CDS             complement(58311..59225)
                     /gene="truB"
                     /locus_tag="PKL11_00595"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNZ12121.1"
                     /translation="MGKPFQDPNDAISGVLVVDKPVGLTSHDVVQVIRKGTNLRRAGH
                     TGTLDPRASGVLVILLGPAVRLSEYVSASDKRYQAVIELGVSTDTYDADGEVLTSAPV
                     DNITEEQFSEALAGFVGEIEQVPPPYSAIKIKGRHAYDMAREGEEVDLAPRKINVYSL
                     ELLEWDPPEAVIDVYCSSGTYVRSLANDLGTKLGCGAHLVGLRRTRSGRFTLRDAIPL
                     RKLREAFDNGTWGQYIIPAAEALSDWPALVLDEKQIDEVRHGHIVAGEGAAEAMVRGV
                     TADGELVALLVYDAEKGGWQPKKVFVAS"
     gene            complement(59279..60253)
                     /locus_tag="PKL11_00600"
     CDS             complement(59279..60253)
                     /locus_tag="PKL11_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNZ12122.1"
                     /translation="MFEPATALDDQIRTLFANAERILILSHIRPDGDAVGSTLGLGLA
                     LQAAGKQVQMVLADGVIRTFKHLPGADQILQQPAGEFDVLVTLDASDPRRLGEPLEGR
                     TPDLNIDHHVTNLNYGRLNLVVPEAVATAAILAERLPLWGLSITPDAAAALLTGLLTD
                     TIGFRTSNMNADALRLGAMLIDHGADLPELYRRALIVRTYSATRYWGLGLSRLDRKGR
                     LCWTSLTLEDRKQSGYQGNDDADLTNILSSIDECDIVVLFIEQREGTVKVSWRSQPGY
                     DVSQLAFSFGGGGHSAAAGADIPGTLDEVRPRVLQATEALLKAAPAAA"
     gene            complement(60253..60657)
                     /gene="rbfA"
                     /locus_tag="PKL11_00605"
     CDS             complement(60253..60657)
                     /gene="rbfA"
                     /locus_tag="PKL11_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNZ12123.1"
                     /translation="MPSQLRLQRIADRIRQELSEMLVMSNVRDPRLTGVFITDVKVDR
                     ELAFADVYFSAVEGQERAKEILAGLNSASGFLRRMLAERVDLRVFPRLRFHWDPTPER
                     ADRIERLLAEIRPDTPTPPPADDAADEDEDAE"
     gene            complement(60721..62505)
                     /gene="infB"
                     /locus_tag="PKL11_00610"
     CDS             complement(60721..62505)
                     /gene="infB"
                     /locus_tag="PKL11_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ12124.1"
                     /translation="MSEQGQKQIELPAVIIVRDLAAKLETSPIKVMKILMDVGVMANI
                     NQQVDFDTAAVVASEMGFEAHPEAIDIVEEKETGEIPLWRQLIANEDQRNLANRAPVV
                     TILGHVDHGKTTLLDAIRHTNVAGGEAGGITQHIGAYQVEHKGHTITFLDTPGHAAFT
                     AMRSRGAQGADVVVLVVAANDGVMPQTREAIAHARAARVPIIVAMNKVDLDGANPDFV
                     KRQLADMDLVPDEWEGNTLVVPVSAKLRRGIEDLLEAILLVAENMDIQANPNGRTIGT
                     VIEAKVDRAKGVVATLLVQNGTLSVGDIVLAGTAWGRIRAMFDFRGRKLQKAGPSTPV
                     QIMGLSEVPEAGDLFQVLPSDREARQVVEDRRTKAQAQRAAAPKATLEELFSRVQSGE
                     ERELRLIVKADVQGSLEPIINELNNLGKESTIGLNVLHAETGNISENDVMLATASHAI
                     VIGFNVQADLAARRLAETEGVSIRLYNIIYRLTEDIEKALKGMLAPEIKEVVVGRAEV
                     LAVFKISKVGNIAGCKVKEGELRRNGKVRLLRAGETLYEGDMSSLKHEKEDVNVVRLG
                     FECGVALRNFTNIEPGDVVECYVLEKSL"
     gene            complement(62658..62972)
                     /locus_tag="PKL11_00615"
     CDS             complement(62658..62972)
                     /locus_tag="PKL11_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNZ12125.1"
                     /translation="MTKKVKPRVKHTPQRTCVGCRLVMPKRTLSRVVRTPEGVQLDPS
                     GKANGRGAYVHNSRDCWQRALKGALANALRVELSDSDRTQLEAFVQTLPEETPPAPVV
                     SA"
     gene            complement(62997..64901)
                     /gene="nusA"
                     /locus_tag="PKL11_00620"
     CDS             complement(62997..64901)
                     /gene="nusA"
                     /locus_tag="PKL11_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNZ12126.1"
                     /translation="MKNEFTLAFNEVLEEKQLPKEVILKALEMAMVSAYRRAVNASAA
                     QHVEATVDVDTGKVSVRAEKEVVEEVVDPHTEVPLAEARKVNPDAQLGDMVIVDSTPA
                     DFGRVAAQTARQVIQQRIRDAERQAQIDHYEKQIGEIISGVVQAVNATGLTIGLEMKA
                     EGTMPRKELIPGERFRIHDRVRALLAEVKDSPRGPQIILSRTHRNFLRRLLETEVPEI
                     FHGVVEIRSIAREPGQRAKVAVSATQSGIDPVGACVGIRGVRIQAIVRELHDEKIDVI
                     EWNADPAIFIAKAISPARVTGVYLNDQSVNGKTATVVVPEDQLSLAIGRDGQNARLAA
                     KLTGWRIDIKSLPEAASEALKNITEDASLAAIVDAALVEKISDMLVRKAEGRSLSPEE
                     YDQMGQFVDRVQRRAVRFAAEKPADTVEARQSHPDIPASAYETNILDSGLPEHVCYIL
                     QEAGYATVSDLALQMKKMPDDILRLNGIGRKAMDEINKLMTALAEKAQADAAAVAEAE
                     AAPAAVEVEVEPVAQPEAVVVEVEAEAAPVQPEPEAEPVAQPEAVVEPAAEAETEAEP
                     SLEEIFSLRPETLHPIAVDEEEEEDSKGKPGKKKPGKKKRRPVVEYDPDTGAAVYRSP
                     HQESDDDWEL"
     gene            65125..66516
                     /locus_tag="PKL11_00625"
     CDS             65125..66516
                     /locus_tag="PKL11_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12127.1"
                     /translation="MRLYEILILAGNGLLAVLMLLRVRRALPLAAGALTLLATLLHVL
                     LEGTRWQMIPLYGLTALLIGLTLYRARQNPAPRRRWRWVWIVAAAACGILPALMPVPK
                     LPQPTGPYDVGTTTFYWVNPDAVDPYTGEVRRLMVQAWYPTDKPSTAEPAPYFPDLKV
                     MAPAIARNFGLPDFMLNHLELSQTHTTLEPPFASDLTQTPVLIFSHGWTGMRYQNTFQ
                     VEELVSHGYVVIAADHPEGAVITIYPDGDVIFANQAALPRSAPAAEFNAAARIVGQMW
                     VSDLRFMRAQMDDLQSGAIASPLAGHLDLARVGAFGHSTGGGAVAEFCYNNEDCQAVL
                     TMDAWMVPYDRVVPAHGLTQPALFMRSEKWSLPGNPPLAEALYENSHSPAYLVEIADT
                     RHFDFTDISQLTPLLTLMKLKGTLDSDRAMTIINTYTLAFFDEALRGDASGVPALEGL
                     YPEAPLEINTGAP"
     gene            complement(66519..67193)
                     /locus_tag="PKL11_00630"
     CDS             complement(66519..67193)
                     /locus_tag="PKL11_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNZ12128.1"
                     /translation="MTLHKLSPSDLTFLWDECPRCFYLKVVRKFGRPASAFPAIFSKI
                     DSLMKAYFEGRSTTAIYPGLPPGKVQFGERWVQSQPITLPGHVDSCYLLGKFDTVVSF
                     EDGSYGVVDFKTSQARPSHLAFYGRQLHAYAYALEHAAGGKLSLSPISRLGLLVVEPI
                     QMTQISDQIAYLGSVTWQEIPLNEAGFLEFIGKVLAVLEQPEPPEAAPNCAWCQYRAE
                     VRQRDD"
     gene            complement(67303..68541)
                     /locus_tag="PKL11_00635"
     CDS             complement(67303..68541)
                     /locus_tag="PKL11_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HNZ12129.1"
                     /translation="MSEHINNVTRRKQIILNVLRGLHEGKSLEEVKAEFGALAAEISS
                     AEIAEVEQMLMNEGLPAEEIQRLCDVHVAVFRDNLDNQRPPEAIPGHPVHTFRAENQM
                     VLRLLDAMTADLARWDAEGDSTARPLAASLENLMQLDRHYLRKENMLFPYLEQVGFRG
                     PSTVMWGIHDDIRGALRQLKSALAEATPELKKVSALFNAVAMMIREMVYKEEKILFPA
                     ALERLKEADWVAIRDQETEIGYCLVTPGNEWMPRPQDLHAADPQPPAQPQGSLPEGLL
                     ALSTGALSLEQINLMLCNLPVDITYVDENDEVRFFSQTRERIFDRTPAIIGRTVQNCH
                     PPQSVHRVQQILDDFRAGLRSVAEFWIQMGGKFVHIRYFALRDAAGKYHGALEVTQDV
                     AGIRALQGERRLLDDAPARP"
     gene            68656..69369
                     /locus_tag="PKL11_00640"
     CDS             68656..69369
                     /locus_tag="PKL11_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ12130.1"
                     /translation="MVPNLPLKPELLRQTELFAGLTDTDLQTIQQAATVRQVERDGFF
                     FYQGDPAEQVLALTEGRVRLAQVTPDGQQVILNLISAGALFGIIAAIKATDYPVSAQA
                     MEDSTALAWSREAMMGLVGRFPLLGMNIMRLMAEHVQETQNRLRELTTERVERRLART
                     LLRLAAQTGVKTPEGVEIGLALTRQDLAEMIGATLYTVSRILSQWEAQGLVRSGRERV
                     MIRFPHGLVSIAEDLPQRE"
     gene            69436..69660
                     /locus_tag="PKL11_00645"
     CDS             69436..69660
                     /locus_tag="PKL11_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNZ12131.1"
                     /translation="MSQPLDPQCSVATLLRQWPAAVPVFLQRRMACVGCSMAAFETLE
                     DAARVYKIQPAQFLEELQQAIQNSALEEKP"
     gene            69657..69980
                     /locus_tag="PKL11_00650"
     CDS             69657..69980
                     /locus_tag="PKL11_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ12132.1"
                     /translation="MTTPYQYFASLPATVDSIPAESIVSRELHKDDRVKAIVFAFAPG
                     QELSEHTASVPAMIQILEGECTLTLGKDSFEAKPGAWAFMEPNLPHSILAHTPVKMLL
                     IMLAK"
     gene            70070..70738
                     /locus_tag="PKL11_00655"
     CDS             70070..70738
                     /locus_tag="PKL11_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNZ12133.1"
                     /translation="MIPETEIFIRLLVAAALGALVGLERERQNQPAGLRTHIILVIGS
                     ALAMTLSINLATQYREIAPNGDPARLAAQVLSGIGFLGAGAILRYGTNVKGLTTATSL
                     WTMAVVGLVIGAGHYWIAVITTILLLAVLTILNLLETHWLSAYLVRVITISCDVRPRI
                     KEDITAALNKITKRVRLASVERNLRHARQQLKYTIRVSERDDLTDLESALSAIEGVRS
                     YMIE"
BASE COUNT        14762 a        18933 c        22001 g        15021 t
ORIGIN      
        1 aggacgcggc cctctcaagg ccaaaacagg ggttcgagtc cccttggggg cacaagagat
       61 ttaggatttt agatttagga ttgctgattc gcgatcaaaa agccctctcg acaagagagg
      121 gctttggctt ttgttgggat aaaccaggag tgggcgatca atcttgcgag gggacaaagg
      181 cttcaacccg ggcgccaagt tcacggatgt tgatgggttt ggacatgtag acatcacaac
      241 cggcagagag ggctttgtcg gcatcgccct tgagggcgtt agcggtgatg gcaataatag
      301 gggtgtaggc cagcgccgtt ttcgagctgc tgcgcaggcg gcgggtgact tcgtagccat
      361 caatatcggg caggttaata tccaccagga tcaggtcaac ctcctgggtt tcggcgattt
      421 gaactccgtc caggccgtta actgccgaga gcaatgtgta gccccgcgat tccaacacgc
      481 gccggaccaa gagcatgtta tcgggattat cttcaatgta taaaatcgtg tgccccatca
      541 tgcacctcct tgaacattag ttggccgttc aaatccgcca gataaaactt ccgcaggggc
      601 ggcgagtgag tgagattgat gcatcaatgc tttggtttcc ataaactttt gtgcgctcat
      661 tcgtccaatg aatttggcgc ggggatgtac ttgttgacgg catcccgcaa atcttgaata
      721 tcaaccggct tggtgagata catattgcag cccaccgaca gggctttctg atcatcgccg
      781 accagggcgt tggccgaaag gacaataatg gggatggagg ccaggcctgt tttctgactg
      841 gagcgcagcc gccggatgac ttcataaccg tcaatatccg gcaagttgat atccagtagg
      901 atcacaccca cgttttctgt ctcagcaatg cgaataccat ccaacccggt gttcgattgg
      961 aacagaacat accccgaagc ttcaagaata cgcttcatca ggagcatatt gtgcggatta
     1021 tcttcgatgc ataaaatggc attttccatg atttgcacct ggccttactc tttatccttt
     1081 tcttcttcaa tcacttgcac aacctgctca acaaacttgc gcgagggcag cgaacctttt
     1141 tgatacatgg cttcgatgtg tccgctgagc cgctggcgtt cttcaggggt aatatctctg
     1201 gcgctgacca caactaccgg gatattgcgg gtgagttcat cggatttcag cgcctccacc
     1261 agtccaaagc catccattcc gggcatggtc aggtctgaga cgatgaggtc ggggcgtttc
     1321 tggcgggcca tcttcaggcc ttcgatgccg tctttggcat ggaaaacccg gtatttcttg
     1381 cgggcttcca acaggcggcg gatcaacagg gcatcatcgc cattgtcgtc gatcaaaagc
     1441 acggtggtgg ttttctcgtc cagattttcc agggcagcct gcaccccttc tcggggagcc
     1501 gcggcctgac cttcactgag atgaacatcc acagcccact ggtcgcccag gacgtgcttt
     1561 tcaaccggga aggtatgccc cgtgcagttg atgaccacct gttcgtccgg gcggataatg
     1621 ccgttacgga gcatcttttc caacccagca aaggccaccg cagtggcggg ttctacagcc
     1681 atcccttcgc ttttcgccag tgaccgcatg gcggcaaagg cttcggcatc agtcacggat
     1741 tccatggtgc cgccaaactg ctgggtgagg cgccacagat aattgtacga gctgccgggg
     1801 tcacccgtgg ataggataat gatgcaggtg tcggggatga caggatcggc ggtatcttta
     1861 ccggctttga aagccctgac catgggggcg cagccttcag tctgaatgac ggcgagcttg
     1921 ggaatgcggt caatcaggcc catgctgaac agctctttaa agccctgata gacccccagc
     1981 gggccaagcc cgccgctgac ggcttgaatg taccagtcgg gtgagcgcca gttcaactgc
     2041 tcaacgatct cgtaggcaat ggttttcatt gattcgcggg cggggagtga accggcgccc
     2101 cggtcgagga agagctggcg gcgctgggca aactgggcag caatctgctt ggtctgatca
     2161 tagttgccgc tgacgcggat gacttcggcg ccaaacaggg cagcctcgcg cagtttttcc
     2221 tggggaacca ggctggtcat aaagacccag agcttgatgc cggcgcgggc gcaggctgcg
     2281 gcataggcta ccgccgcgtt accggtggag gcgatgacgg cctccttgac gcccgattcg
     2341 agcatggcgg ctacaccaac cgccgcctgg cggtctttga aagaactggt ggggccgtaa
     2401 cgttcgtctt tgatgtagac cgtattatgt cccagggcgg gggcaaagcg ctgcgacagc
     2461 cagagcggtg tgccgccggc tggatacaaa ttgatggctg caggggcttc gacgggcaaa
     2521 atatcctgat agcgccacag gttggccggg cgaccgggca ggccgcgcag gatattctgt
     2581 ttgaatgcga cataatcata gcgggcctct aaccactgcg agccgcactg ctggcaaaca
     2641 gctgcgacca gcggcacata aggctgctga tgaccacaga ggacgcattc caaatacttg
     2701 agctttgcca tgttacttcg cgacaacctc aatgggttcg gagattttag cctctcgggg
     2761 taagattaca tagaaggttg aaccttcccc agggacattg ctgctttcag cccacatcct
     2821 gccgccttgc atttcaacca ggcggcggct gatgggcaag cccaggccgg tgccgcccac
     2881 tttgcgggtg gtggatgtat caacctgtga gaattcctgg aagatgcttt ccagatggtc
     2941 gggggggatg ccgatgccgg tatcccggac ggatatcatg atctgatcat cgggcatttc
     3001 ttctgcccga atggatacaa atcctttttc agtgaatttg atcgagttat tgaccaggtt
     3061 gatcatcacc tggcgcaagc gggtaacatc cacataaatt tcaaggtcgg tgttggaggt
     3121 ggattcaatg aagacatcca gacctttttc gccagccagc gaagaggtaa tgttgatgac
     3181 ttcggtaaag acgtcgctca gcttgacctt ttcgggatgc aggttcatgc gcccagattc
     3241 aatcttggcc atgtcgagca cgtcgttaat cagatgcagc aggtgctgac cgtttttctg
     3301 aatcaggcgc aagtcatttt ccatgtactc ggtcagcggg ccatcaatgc cttccagcat
     3361 cacatctgag aagccgagga tggagttgag cggggtgcgc aactcatggc tcatgttcgc
     3421 caggaaggag gatttgaggc gatccagttc ctgcagttcg aaggcacgcc ggcgcagttc
     3481 ttcgttcaac cgcagggttt ccagatggac gctggcctgg gtggcaattt cagccatcag
     3541 tccggcggct tcgggcgagg ggttgttcaa cccggcgaca gccagttcgc caatcacttc
     3601 gccgcgcacc atcagcggtt gagacaggct gacgttctgg ggttgagatg tcaacggaga
     3661 gacctgattc aggtcatata catagccagg ggtcagatcg gcattggcct gggcaaaacc
     3721 gctccagcct tcacgcgtca ggcggcggct ggcatcttcg gcttcggcgc gggcccgggc
     3781 agcatctgcc agcaggcgca ggttttcaac ctgctgcccc acctggcggg ccacagccga
     3841 gaccagggcc tgctcgtcat ccgacagcgc atgaccttca tcggtttgga gcatcacacg
     3901 gcccacctgg gtgtccataa cctcaaccga ttcctgatag gttacctgag gcggggcggg
     3961 gtccagacag ggctcaacgc cgttctgatc ataggcatag ccgatgcgtt cattttggtg
     4021 aatgccatcc aggtaggcat cccagctttc gtgaatgaaa cgccgggtgg cagcctgagc
     4081 ttcagtacgg gctcgctcag cagaagacag caggcgcagg ctttgaattt gcagcgagac
     4141 ctgctgggca actgcctcaa tcaggctctg ttcttcagaa gtccaggttt gggatggcgg
     4201 aggctccacc accaccgcgc caatgggctg cccgctggta gtcaggggcg ccaggatggc
     4261 gtttttgaga tccacctgct tcaagacaga ttcgcgcacg cgctgaaggg tgttgccggt
     4321 gaaggcgtag cctacctgct cgcgctcata gataatgggt gcctggggca gactgatcgg
     4381 cgccgcaggt tctggcaccg ccggggcagc cggttccgac gcggggataa ggacttccgc
     4441 tggttccaga ggctggggct gggctgcctc caggtcgctg ccctgaactg gcgcggcatc
     4501 tgtctccgga tgggcttccg ggcgatcaaa ccccgagaac aggtcatgga gccgagtatc
     4561 aatcttcttc ttgggcataa gtcatggcct cctcagcctg gctgcgctta ttcctggtcc
     4621 ttgatgctca gttgggcaat ggttcgcggc gcaccgaggg cgtgtccgag ttcgcgggca
     4681 gcaatttgca gcacggcttc aaccgttgtg gcgctctgaa tcttctggct aatgaggttc
     4741 aaagtactct ggcgttccgc ctgcttttga gcacgttcaa actggcggcg gttttccaaa
     4801 acggtggcaa tctgcggggc catggcgatg aacaggcggg tatctgcagg tgagaacttg
     4861 tacggctcct gaccttcaag gagcaggaca cccacctgcc gcccggcaat gaagagcggc
     4921 atggaagcca tggcgcggat gttttgatgg atcacaagct ggcgtgggcc ttcaatcacg
     4981 cgcgggtcgt tctgcatatc gttcgagaag agcggctcgg ggatggtgaa gtaggcctgg
     5041 gcgttgatct cttccagggt gtagcggtgg ccgatttcgg tgggttcggt gccaacgccg
     5101 ctgtaccagt tggcggcaat ttccatgtct accagttcgt tggcggcatt gtagttgaac
     5161 acgccgagca gctggcggtt aatcaccggg atgttcaggg tgtcatagat aatttggagc
     5221 atatcctgaa gatcactggc ctgagccagg cgcaagcctg ccgctgacag gttttcggtt
     5281 tgctccagcg cggctttggt ctgagcctgc tggcgcaagc tttcaatatg tgcgcccagg
     5341 cgttcggcca cagtattgat caggcccaga gcgtcgttat cggcagtatc gaggccctga
     5401 accatcaggg tgcctacggg ttcatcgcgc actttgaggg gcagggtgac gccggtttcc
     5461 tgagccagtt gatcgttatc aaacgcaacc ggtcggactt cagacaggtt atagaggaag
     5521 cccaggctgt cacgcccggt gacatccagg tattctttcc agccttcacc ggtcatacgg
     5581 cgggagactt tttccgcttc ggcgcgggcg cgctctgcag attcgagcag gcgcaggttt
     5641 tcgatttgct gcgccacctg gcgggccacg ctgttgacca gttcttcggt gcgggccatg
     5701 gggggttggc ccgcgagttc aaccaccagg ttgcccagca cttcgccagt tacggcaatg
     5761 ggtgcagcaa aggcggctcc accttcggcg gtgggggttg tttcagatgc gtccagcggg
     5821 gcaacttcct gcccatcaaa cacatagcca aatgattcgg gcacgtgaac ggcatcaagg
     5881 tattccaccc agttggcacg cgccaggcgg cgggcctggg cttccacctc agcacgggct
     5941 tgctgggttt cggccaggga ggaggcgttt tgaatggcga tagccaactg accggccagg
     6001 gcttcaaacg cggtgaggat ttctgtgttc agtgtgtttg gctcgctgct ttggagatcc
     6061 agtacaccca ccacccgttc gcccaccatg agcgggatgg ccatttctga gcgggtatca
     6121 gggagcaatg ggttgggttt gaaggtcagg ctggcggctg tatcggccac cacaatagaa
     6181 tgtttctcga tggcagcgcg gccattgatt gaagaggttt gcaggggcag gcggtgattg
     6241 cggctgagca gttcggcgcc caccgaaccg gtgcccgatt tgaggaccag gctgttctga
     6301 atggggtcag tcagatagac ctgcacatag tagaggttga agcgtgaacg gatcagttcg
     6361 gcagcgtctt tcaacatcac atcgagggcg cgcacctgcg agaccgagcg gccaacttca
     6421 gcggccagct ccaggctctg ggtacgttct gccacgcgct gttccagggt tgtaaaggat
     6481 tctcgcaacc tgccagtcat attattgaag gctgttgcca gaacaccaat ttcgtcctgg
     6541 ctcacgactt cggcgacctg attcaaatcg ccaccggcga tggcggtcac ggtatcggtc
     6601 agcagaccaa ttggccggag gagccgctgg atggcgattc caaccagaat ggccatcgcc
     6661 agcgagccca gcaggatcag gataccagca acggtctgga actggcgcac gggagccagc
     6721 agttcggcgt tgggctgctg agtgataatg ccccagccat tctccaagcg tcccgcataa
     6781 gcattccatt tgacgccact atcatcggta aacgtcaact gcccggtttc gccgcggata
     6841 agagctgcaa cgggtggata ggcgctgaaa tcggccagtt tttccgtggt gtaggtggga
     6901 tcaggatggg caaccatgaa accattcgca tccacaatga aggtaatggt accgccctct
     6961 tcagtgctga ccacttcatc gctgatttcg gtcagttcac tggtgtagcc caccacgccg
     7021 acaatctggt cgccgctgta gattggggca gacatggaga gcgctggctt gttgattgta
     7081 cggctgatca gcacctcgta agttaccggg gtgccaccgc gagccgcctg gaaccaagca
     7141 cggtcactat aatcggtttg agcaccggca tcgctgcggg ccacgttcag gccgttcagg
     7201 tccagggtgt gaaccagaaa gaggttggga taggcctggg taatgacttc caaaaccggt
     7261 ttttgctggg tggcccgcat ggccgtaatg ctgggaagca tggtcgtttg cttcagagag
     7321 cgggtttgtg aatccagcca ggtggaaact gtggtggaca gcgaaagcgt tttctcttcc
     7381 aggtgcagca aggattgatt ttgaatttcc gaacccgccc gggaactaat gtacacagca
     7441 acaccgatca agagcgggat agtcaaaatc attaccagag ccgtcagacg agctcgaatg
     7501 ccacggttgc ttaaaagtgg cttcttagaa gaattcgcag ctgttgtatg ggtcatattt
     7561 cactctccat aattatcaga ggaggacatc gtctgacagg ttaattaggc tcaatttggt
     7621 cagcatcggt ctggcgcaca ggcgcaatgc tggcgctgac acgcgaggcg ctcaggacaa
     7681 cgcccagttc acggatggcg gtttgcagag cttcctggac agagctggtg cgctgaattt
     7741 tctcgccgat gtcgctggcc agggcctcaa agtcggcctg agctttggtt tgctcaaagg
     7801 agcgcacgtt ttggatggat gtagcaatct gagcagccag ggtggtctgc acgttgacat
     7861 ccgattcggt gaagcggtcc acctggtcgg attggatatc aaacacgcca atcaccttac
     7921 cgccaacgat catgggaaca gccagctcag agcgggtatc gggcagcagg gggttgggca
     7981 ggaagtcggg cgcctgggta acatcattga cgataacgcc tttgctctca cgggcagcgc
     8041 gtgccaccaa cgactgttcc cgatcgagcg ggatggtgcg tttttctgct accatcacac
     8101 ggcccggttc accggcgccg gccgtcagta ccagattttg accagtctca tccagcaggt
     8161 aaatatgcga gtggtacagt ttgaagcgtt ctttggtcag atcgaccacc gattggagca
     8221 gtcggtctgt ttccagaatg gtggcggtgg ctgtagatac ctcagccacg gtctccaggt
     8281 cctgagtccg agcggccacg cgctgctcca acgcgccaat ggacgcccgc aactgggcgg
     8341 ccatgctgtt gaaggttgag gcgagcaggc caatttcatc ctggccgcca acctgggctt
     8401 caacatccag gttgccgcgg gcaatttctc cagcgacagt ggtcagagcg cccagcggtt
     8461 tgaccagacg gttagcaata aagaaggcgg cgaggaaggc caggatcagg atgaccaaaa
     8521 ccagtacagc acccagcacc agcgagttgc gggtggattg ggcaatctgc tcctgggctt
     8581 cggtggcgcc agccagcaat tcttctgtgg gaactacaat ggccaagcca tagccgactt
     8641 ctgaaaccgg ggcgtagatg atgaaatttt caacgccgtt gatggtgacg gtttccagcc
     8701 cgctttcgcc agtcgttaat ttttcaacgg cagcagcaaa atcgggggag agttttgaca
     8761 gcacagcctg gtccagcacg ttgccgaggg ggtaggcatc gggggtaatg ccaaagtcag
     8821 cataggcggc tgcgggcatg gcgatgaggc gtttgctttt atccagtagg aaggcgtgac
     8881 cagtctgacc aactttaagg ttgccaacaa ccgctgtaat ctggttcaac tgaacatcca
     8941 tgccctgcac accccggaac tcaccggagc tgtcataaac ggggtaacta acggtaatga
     9001 tgaggccgtt ggtggcggca tccaggtagg ggtcagacca gacggcgctc ttagtggggt
     9061 tttgggctgg cgatgcagca ataaaccagg ggcgctgggt gacatcaaat tcagggggca
     9121 ccagggctgc cagatcgaca ttggggtaat acattgtctc gccgggggtg ccgccaaagt
     9181 aagctgccac agcgtcgggg ttggctgcca ggatggccgg cgtggtgaag tcaagctgaa
     9241 ccagcgtatt taactgggaa accagatcgg gggtcaactc taccgttccc ggaataaaga
     9301 ccgagaccac gtctgaattt gggttatccc agttgctgtt cgacaggtga tacagcacgt
     9361 cttcagcatt ccagtagtcg ccggcattga gtttggattc gttatccaat agttttacgg
     9421 ttgtatcgcc gagggtggta atttctttgc gcagggcggc aaaaaagttg ttcagggttt
     9481 ctgtttcagc cgcaatgctt ttttgcagtt cggtttcggc ctgctggcga acggtggtat
     9541 ccaactgctg ggtcaggtaa aggtcggtct gctgggcgcg ataaaagacg tagaatccca
     9601 tgccagcaac tgccaggaaa ataatcacca tggatgccag gatcaggcgg gcgcgaaaac
     9661 tgaacgtccg gcgcggctga ctggcagttt gatttgagtt agaagtggtt gttaggccca
     9721 ttttgattct ccgtaatcgt ctgggggatc cgtttatttg ccggattcgg ctgtcagggc
     9781 atcgggatcc aggattactg aagtgggttt catgcccagg gcatggccca attcacgcac
     9841 cgcaatctgc agggcatctt caacactggt ggtgctctgg atgcgctggg tgatcaagtt
     9901 gacggcgctt tcacgcacgg cctgattctg gatctgttca aaggagcggg cgttctgcag
     9961 ggcgacaccc acctgagagg ccagggtggt ctggatgctg acgtccacgt cggtgaagtg
    10021 gttggcttca tcagactgca catccagcac gcccaccact ttattggcca ccagcatggg
    10081 gacggccatt tctgagcggg tgtctggcag aaggggattg ggcaggaagt ccggagcaag
    10141 ggtgacgtcg ttgaccacca caccttcacg ggagcgggcg gcacgcgcca ccagtgattg
    10201 ctcacggtcc agcgggatgg tgcggttttc agcaaccatt tgacggccca cctcgccggc
    10261 gccagccgtc aggaccagtg tatcgccagc gtcattcaaa aggtagatat gggcatgata
    10321 cagattgaag gattgtttgg agagatccac cacttcttgc agcaggcgat cagggtcaat
    10381 gatcgtggag gtggcaatac ccacgttagc caccacgctc atgtctgagg cgattttctg
    10441 cgtgctttcg tactggcgga tgttctgaat agccacagca atctggttag caatggactg
    10501 caaagcgtca atgtcggctt gctgcaaacc attcgccacg ttttgctgca catccaaaac
    10561 gccaagaacc cggttgccca gggtgattgg cagcgcaatt tcggattttg tttcgggcag
    10621 gaaggggttg ggcagccagt cggggttttg cgaggtatct ggcaccaaaa cgggttcgtt
    10681 ggtttcagcc gcccgcccca ccagaccacg cccggcccgg attttgtgga actgggccag
    10741 gagcttttca ccggcggtgc cggtgccacc agccatcacc aggttttcct ggcgatcatc
    10801 gaagaagtaa atctgggtgt gatagtagcc gaaagcctca ttcacctggt ctaccactgc
    10861 ggcgaccagt tctttttgct ccaaaatggt ggagaggcgg cggctgactt cggtggaggt
    10921 ggcgagcgct ttggtgcggt cggcaacgcg tttttcaagg tcgaggatca gctcacggat
    10981 ctgtttcgcc aggttattga aggtgcgcgc caggactgtg aattcatccc gcccggccgt
    11041 gctgacttcg gcttcaaggt tgccgcgctg aaattcttca gtgccaagcc gcagggcttc
    11101 gagagggcgg gtgatgctga tggtattgaa gacagccaac aaaacagcca taaccaggcc
    11161 cacaataatc aaaatcacac cggtgcgaat gctgttttgc gcctgagcca acacgtctgt
    11221 attggtggcg gtgacctgct tgacgttttc atccgccatg ctttccagcc gcacgctgat
    11281 atcgttgccc agacgttgta actgcgattc caaacgggtg gcctgggcgg catcgccggc
    11341 atcctgagcg gtttgcagtt cgccaatctg agtctgatag ttggtgagag aggtgataat
    11401 cagggtcagg ttgtcagcgg ttgccttgtc aatatccagc gccagacctt cgttcagccc
    11461 aatcaacgcc tggtcgacat catccagggc ttcgtaaggg ctggggacgt agtcttgaat
    11521 gtagcggttg aggttcaact gggatgacgc cacacggcgg gaggtggtca gcagcagacg
    11581 ctcaacctgg gcatccacgc tggtgaagcg ctccaggcgg ttgaccaccg agttgaagtt
    11641 gcccaggatg gtgattgaga ccaggcccgc cagtaccaac accagcagga acgcaacgat
    11701 cagacggata cgaaccgtcc cctgtcgcgc aaaatcaaaa aggcgattta acatagccaa
    11761 cctcctaggg tttggtctga gcctgttccc aaaacgtatt gttgaagaac aaatcggggt
    11821 taacctgcgt atcgaggacg ttgtaagaat agagggtatc aatcgtccgg cggacgtcgt
    11881 tgcggtcggt ataacctacc tggatcaggt cgctttggac gatcttgatg atttcgttca
    11941 tcatggagac ctgatgttca cgggtcaggc tgccgctttc gtcgtatttc atcacaatat
    12001 caaccgcttc ctggggattt tcaatggcat actgccagcc gcgcaggctg gcgcgtacca
    12061 tgcgcaggca caggtcgggg ttgtcttcaa tcatcttttt ggaggtgaac agggtgtcgc
    12121 cgggaaaatc gaggccatag tcggcgtagt cgataatgtt gatgtcttca ggtttgtagc
    12181 cgctttccag aaccacatag tattcgttat aaaccatggc cgaggccaca tcgagttcac
    12241 cattaataaa gctgtccatg ctgaagccct gcgagaccag ggtgaaagag ttagggtcaa
    12301 tgccttcttt agccagcaaa gcatcaaact gggcttccca gttgcccagc cagacgccca
    12361 cggtatggtt ggcaaagtcg gcgggggtgg caatgcccga gtcttttttg gcgatcagca
    12421 gcagaccgtt ggattgctga atctgggcga tactgaccag atcctgggat tgttgaccct
    12481 gtacaataat gtcggcaagc agactggtgc cgaactgggc agagccagtg cggacggcat
    12541 cgagggcgga aatatcgggg ccgccagggc ggatggtgag gtcaatgcct tcttcggcgt
    12601 accagccctt gtccagggcg acataatagc cggcaaactg cgcctggggc agccactgca
    12661 ggttgagggt tacagcagtc ggcgtggtga cctgatcttc aggggcgccg ccattgttgt
    12721 tgcaggcgct caagagtggt gtgatcacca tcagggcgag gacaagcagt gaaaaccagc
    12781 gtttgttcat gttatagctc cttgaattta gagataatgg gggaattcag acagggttgg
    12841 taaaggttgg ataagggctt atccagaagt gtgcttgttt tgcggagggg cagatggccg
    12901 agcgccagcc tgcccggtga gatgggcaat ggcactggcg attagctggc agatccaatc
    12961 ggggcgcacc ggcttgagca gcacatcatt gacggacagc ttttgcgcgg ccttttcgag
    13021 atcaggaatc tcgtaagctg taatcacgat gatgtaggcc gggcagattt cgggcagggc
    13081 gcgcagtttt tcaaccagct gcagaccatt gatacccggc atgatcatgt cggtgatcag
    13141 aacatccacc gggtgttctt tcaccatttc aaaggcctgc tcgcccgtgc tggcaatgac
    13201 cgcctcaata ccgggaccga gcagggaaat agcgcgcgcc agggttgtgg ctgtggtggg
    13261 gtgatcgtca acgaccagga cgcgggcatg gacatcggca ggaaattttt gggttttcat
    13321 cagtactatt ttgcctgatt ctgacccgtt tgacagattg tgtttgaaca ggaatggttg
    13381 ggaaaagttg gtaaagcatt tccccgtgtc actattgttt tggtcggttt gtgggtggtt
    13441 agtcgctcac caggcgatag ccaaccccgc gcacattaaa gatgtagcgg ggctcacggg
    13501 catctggctc aatggcttgc cggatctggt gaatatgcca cttgaccagt tcctgcgctt
    13561 cgcgagcgtc ggctttgtaa ccctgggctt cagccaccag ggtttgataa tcgaccactt
    13621 cgggcgcatg ccgcgccagc accaggagga agtcgaaaga tgtgggcagc aggctgatgc
    13681 tgttttcacc gaccgtgacc cggcgagtgt ataggtccag ggcaatatct ccgcgcttca
    13741 aataccgctc tgatgtgggc ggcggcgagg cagtcggggg caaagccgca ccttcgtcat
    13801 tgagctggtt caattccccc tgcaaggcgt caatctgggc ttgaatttcg cgcttgcggc
    13861 gttctttttg cccggcaagc aggatggcct ggatgcgttc tatgacaatc tcgggtttga
    13921 gcggtttcaa gaggtaatcg gtggcgccgc ggcgcagggc ttctacggca gagtccagat
    13981 cgggcagggc ggtgaagaga acaaccggca atccggggca gcgggtgcga atgatatcca
    14041 gggcatctaa accgggcatg cctggcaagc gaagatccat gaaaaccagg tcaaagtggg
    14101 aggtttctaa aaacgccaac ccctgctcgg cattttcggc ggtggtcact tcaaagccag
    14161 atgactgcaa gatgcgcgcc agtgtatgcc ggatgacctg ctcgtcatcc acaatcaaca
    14221 gcttgcccgt gatgttcatt cctccccttt gacctctgtt accggcagcc agatggagat
    14281 tgtggcaccg cgctgcgggt tgttttcggc ctggatgcgg ccaccatgcc ggagaataat
    14341 ctcctgagag atggttagtc ccaggccggt gccatgttct ttattggtgg taaaggcctc
    14401 aaagatattc gggagaatat cgtcttgaat acccgggccg gtatctgaga cggtaaccag
    14461 ggcttcgtgt gtgtcgggga gatgctgggc ggtgactttg agatgcccgc ctccgggcat
    14521 ggcttcgacg gcattcatca ccagattgag cgccacctgc cggagctggt cttccaggcc
    14581 agagatgggt gggagggcat ggtccaactg gagttcaaac gaaatttgat gatgccgcag
    14641 gtgtgtggct accagagcac ccacttcctg aacaatatcg ttgatattaa ccggcttaaa
    14701 ttcagccgcg ttcacccggc ggtaggtgtg acgcaggcgg gtcagcatgg ttgccatgcg
    14761 atctgtttct gagaggacaa tatccagatc ctgccggccc tggtcagaca gggtttgctc
    14821 ttctttgagt aagaaaaggg cgttttgaat ggcctggatg gggttattca attcatggga
    14881 gacggaagcc agcaggcggc cagcgacaat caacttctca ttatgcacaa gctggtcgcg
    14941 ggtggctttt tcgtgccgca gggcttcctg caggtcggca tagagggaag ctttttgcag
    15001 cgcctcggcc aactggtcgg caacagcagt gactagctgg acatcgcgtt ctgtcaaaat
    15061 catgggcgca acctggtgaa tatccagcag ccccaggaat ttatcgccac tgcgaatggg
    15121 aacggccagc tcggagcggg tatctgccag gtagggcggg cgtacaaaca ccatcagatc
    15181 ttcaacgctg ttggtaaaga aggggccgcc ggtttcggcg gtaaagccca caatgccatc
    15241 gccgggagaa aggtgataac cttcggcctt cagccgggcg ccaatttcgc cgctgccagc
    15301 acgcatgacg atatcaccgg ttttagggtc ggcgacaaag atttgcacat agtgatagcc
    15361 aaagctgacc ttcaacaggc tgacgacttc gtccatcagt tcctgcgggt caatggaggt
    15421 gatcagcttt ttgttaatct ggtacagggc gctggtctct ttatagctct gctgggcatc
    15481 gcttaacagc cgggcgttat caatggcaat ggcggcttcg ttagccagaa tttcgaggaa
    15541 tttcacccag tcttgttcaa cggttaatgg gctgcggtga aagatttcca gaacgccgag
    15601 caaattatct ttggcgacca acgggacgcc caggtaggcg gtaaaatgct cattagcgat
    15661 ggcctgcgtc agggcggggt tattggtcat ttccgccagg tcgggcatga aaaccacctg
    15721 ccgatcctgc gccgcgcgcc cggccaaacc gctgccaata ttgaggtgcg taaattccaa
    15781 ggcgtgggtg atgaagccct gtttggcagc aaaatcgagg gtgcgggttg gggggttgta
    15841 cagcaggatg gcgcaggcat caacatgaac ctgatcgaca atttgctgca caaaaatatt
    15901 cagaatagcg cgcaggtcgg tgacgctggc aatggtgtgt tcaatggtat gcagcacatt
    15961 caggcgctca agctggcggg tcagattaac ctcggtctgt ttgcgctggg tgatgtttcg
    16021 caaggtgagc aggcgcaccg gctggccctg gtaatctata tcgcggcagc tcatttctgc
    16081 ggagattacc gaaccatccg gggtgttcat ctcggtctcg taggtttcga tggcgccggt
    16141 atgcagatga gagagtacgc actgcctggc ttcggtggtg aaggggatga tatccagagc
    16201 atttttgcca ataagctggt ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnggcttcgg tggtgaaggg gatgatatcc agagcatttt tgccaataag ctggtctgga
    16381 ttgggataac ccagcatttc ggcaaatgcc aggttggcat ctagtatgac gccatcccga
    16441 tggatcatca atccttcggt tgtcagcgag gccagatgtt gataacggtg ttcgttggtt
    16501 ttcaaaatac caccctcagg cgtgccggac atatcccctg attcttgctg caatcgtggc
    16561 cgagaatcgt ccatgcacag tctcctgtct gcagatcaaa acattatgag gcattgtctg
    16621 gtttgagaat ttgaaagaca aacctttcct atcaaattca cctgaattat tatactcaaa
    16681 attggaatac aacatattga aaacagatgg gaaattttta ctaaatccgg cagccagggg
    16741 ttcaggattt ggccttggac gcaagcaggc tgggtggcgg atcgccccac cacatttcta
    16801 tgcgccggaa ggcttcaaca taacctgttt tctggagggc cggcaggtgg ggatcgttga
    16861 cctggatatt ctcgagctga gtgtccagcc ggggatacat gtaatgtaaa tgggagagga
    16921 aggcggtggc aactgcggtt ggatcaccca catcggtttt ataggcaaaa cgcgtcaggg
    16981 taaatttttg gcgctgataa accagccagc catggctgcc gtccgggagg gtgacgcgca
    17041 gaccagagac atcacgggcg ttgttgaggg tttcggactg attggtccag ggttgggtgc
    17101 cgcggttgcg ggcaaccagg tccagcgcct ctaccagttc caggcgctgg gcgtcggcaa
    17161 tcacggggtc gggcgggggg agggtgggag aacggcgcag gaccagcagt tcgccaattt
    17221 ccttaaagcc catcttcaag aacagggtgt gcgccggagt gttgttttta atcacttcca
    17281 gcatacagaa cttgatgccc agccggtggg tctgctccag caggccttcc atcagggcct
    17341 gcccggtgcc gtgccggcgg gtggtgggca agacgcccag gcgggtgatc caggtgcggt
    17401 ctttgcgcac gcccagcatg gccagcccaa gaatatcgtc tccctgccgg gcaaccaggg
    17461 agtgatcaag atcaacatca taggtattga catactccgc caggcgggcg gcgttcatgg
    17521 gcatgggcac cagataatcg acgcgcgtct ggttatacag cgcggtgagc tgctcaatgg
    17581 taaattgact ggcaggaaca agttcaattg ccgggctggg tggaagagtc ataggattga
    17641 ttgggcttca aaaaagaggc aatctgggat agaaaatcat tcactttaat gatgggtttg
    17701 gggatatacc ccgcacagcc ataactggtc accatctgac gggcagtggc ctcgggccag
    17761 gccgtgtagg ctacgatggg gatggcagcc gtgcgcgggt cggcgctgag ccagccagcc
    17821 agggtttgac catcataatc gggcagaccc aggtccaaca agatgagatc gggctttaca
    17881 gacacagcct gttccatgcc aagctcggcc tcgggggcgt gaaccacttc atacccggca
    17941 gagaccagaa ttttacaggt cagttcagcg ttatcaggaa tgtcttcaat cagcaggatt
    18001 tttgtcatca agcatctccc agcctcatca actcatgttg acagactgac ttaaattata
    18061 agggaaaatc gcgttaaaac agccgcaatc taaccggctt actaaactgt aaagaagaga
    18121 cagcccgccc tcaggcagca gcttgagggc gggctgtata cggttgagaa gattgccggc
    18181 ttaaccgggc agggcgcctt tgaggaggaa cagagcagcc atggtttttg catctcgaaa
    18241 ttcgccacgc tcggctcttt ggaaggcctg cgcgaccggc agcttttcaa tttgcaaata
    18301 ctcatcggga tcggcggcca gaggcgccga atagagaccg gtagccagga agatggagtg
    18361 aaattcgttg cagtagcccg gggtaaggta aaagccgccc aggtaacgca gctcggcggc
    18421 agccatgcct acttcttcgc gaacttcacg cgcggcgccc gattcggggc gctcgccggg
    18481 ctccagcacg ccagcgggca gctcgagcag ttcaccatct acgcccacgc ggtactggcg
    18541 gacaaaccag atctgtccat cgctgtccag cggaatgatg gaaatggaat cacggtgttc
    18601 aaccaggtcg taatgcttct ggtcgccacc ggggacacgc aaccggaggc gggcgacatc
    18661 aaacgcccgc ccctggtaaa cgacatcgcg atggaggact tcaaatttca cggaatttgc
    18721 agcacctggc ctaccgacaa cgtgtagggg gccgccagac cgttggctgc ggcaatcgcc
    18781 aggggatcta catcaccata aactgcgcag gcaatcttgt agatcgagtc gcctgaccgg
    18841 acggtgtagg tggccggatg cgccgccaga tagcggttgc ccgctgacca gttgcccgtc
    18901 tgggggatct tcagcacgtc gccgggctgg acaatggagt tcttggtcag gccattgagg
    18961 gccagcaggc tgtcggggtc caggtcaaaa cgccgggcga tgcaataggg gctctcatcc
    19021 ttctggatgg tgtagttctg cgggcgagcc ggggcgacat atgtgtaggg gttggctgtc
    19081 ggcgagactg gcggaatggg ggtgattgcc gtaacaacca tcacggtggg ggtaggctgc
    19141 gcggttggct gagattgcac aacaggcgta gtcccgggcg ctgccggcga agtcgggaag
    19201 gaaatttcag aggtggctgt ggctgccacg ggggccgggg ttgaagccgg gagcttgcag
    19261 ctgctgagcg ccagcgccag gataacgata gccagcacag gaatgaggaa tttgcgtgtc
    19321 attgggaatt ctctccttga tttgaatcga ttttgccccc ttgagggtgc aaaattagcg
    19381 aaatttgcga aatttttaag gtttttccca tactagcata accttgactc gccgtcaagc
    19441 ggatgagggg aatgattttt cagcgagagg gcttgacaat tgatggggtg gtcactataa
    19501 ttatggtata cggtataccg tataccatat gacaaacacc ccttccccaa cccttacctc
    19561 tctggataat cccatctcgc tggagatgcg ggcttatgat gcgctgaagg ctgcgatttt
    19621 gagctttgca ttgatgcccg gcgaaccgct ggtagaagct gaactggcgc ggcggttggg
    19681 catcagcaag acgccggtgc gcaacgccct ggcgcggctt gaaaaagaag gctttatcgc
    19741 caagcagcct tataaaggct acaatgtggc cgaaatttca caacaaacag tgattgagat
    19801 ttttgcagtc cgcgctgcgc tggaaggcct ggcggcccgt gaagccacag cccatctgac
    19861 gtccgaggca ctggccgaag cggcagtcct gcttgcagaa catgccgggg cggcagagcg
    19921 cggtgatttg gaacaagctt cgacgcttaa ccggcagttt cacgatctgc tgctgcggct
    19981 ggcaaacaat gcctggctaa cccagatcct ctccaatctg gatgagcatt tgcagcgcta
    20041 ccggctgctt tcagcctttc aggcggggcg gttggagaaa tcggtggcgg aacacgcggc
    20101 cattctgcag gcgctgcaag ctggcgatgc tgccgccgct gaggtggctg cccggcagca
    20161 ccttttctcg gtctcggcag acctagccga gcaggacttt aatcaacttg tcgggctggc
    20221 tcaccaacaa cgcagcccgg ctgtcgaata gatttccacc ttatctcttt tcactttacc
    20281 caaggagttt tgtatgtccc ccaaatttaa ctatcgttac cagctttaca agcacctgca
    20341 ggccaatacg caggccatct atgccgaatc acgcaaggcg gctgaagacc tgggtttgct
    20401 gccagagttt cgcggcgcac tggggctgac cggggccgtc tcgggctgtc acgggctgct
    20461 ttctcgggcg gtggaagagg ccgttaatga agtctctcgc aaggtgctgc ccagtgcggt
    20521 gctggatgaa caactgcgcg acctggtgaa atcttactat ggtgatgaat atgacgcggc
    20581 gctggtgaat acctgcgagg cagcgttgaa tacggccttt gatgtgctct gtatgccgcc
    20641 cacgctgggc cgcggcgagc cttaccgcac ccgctatatg gcgctctacg aacggcattt
    20701 acaccatcag ggcgcctatg gccggccgtt cccgcccaag tacaaagaaa tcaatgccga
    20761 gcgcggcgaa gctgctggcg aatacggcgt gcagggcaaa cgcgccttca acctggatac
    20821 gctggtggtc aagccggtcg gtgcgacgta tgaatgccac ggcatcaaat tcaacccgat
    20881 ccccaacatg ctgcacgtgg atgcagcggc gaccattgaa cgggtagctg aagcctgcgc
    20941 ccgccagccc gaactgatgg ctggcatcgc ctcgctgggt tatgataccc ccggctatgg
    21001 ctacgggcag aaggacgcca acggcgctcc gctgctgcaa gttggcctgg cggaaatcgc
    21061 gaactgctat gatattcctt atattgtaga taatgcctgg ggcgtgccct ttattggtac
    21121 cgatattcgc aagacgggtg cacatgtgat gatgtattca atggacaagg cctcgggcgc
    21181 tccgacctgc ggtttgatca tcggcaagga agaagagatg gttttgatgc gccgggcgct
    21241 gggtattcat ggcgcgcggt acggcacgct gtcttcacac ggcaaggcct cgcatgtggg
    21301 ctttgacccc ggcaaggaag cactggcggg cgccatcgcc gccatgcgca tcctgaaaga
    21361 tcagcctgaa atcacgctga aggcgctgga tgacctgtac acgattgtga tggaagaatt
    21421 tgaatcactg ccgaccccgc tgcgccaggg ctgggtgatt tacaagtcgg tcaactgcca
    21481 atctgtcgag ctgaattact acgattcctg ggccaacggc gcctggggct tgccggtctt
    21541 ctcgattgag gatatgtatg cgggcagcca tctgctgcag aacgccatgt cacagatggg
    21601 tatgatcccc accattgcgt atgacgccaa cattatgatc agcaacggcg tgggcaacct
    21661 ggacgaagaa ggccgtctgt tggaacgccc aacccgcctg gcggtgcgcg gcatgttcaa
    21721 gattatggag attctgggcc gctacgccgg aatgctcgac taacttcaac ttcttctttc
    21781 tccttcaggt gcggtttttg ctttgacaaa aaccgcaccc ttattttaag gttacggatt
    21841 tgcgattccg tgtaaaataa tcatactgta ctttcgcaaa ccctgtaccc tggaggaaaa
    21901 tacgatgagt tttcacacaa ttgttgttgt tgggcggctg ggccgcgacc ccgaaatgcg
    21961 ctattcaccg agcggcgacg ccgtgacgag tttttccatt gccactgacc gccagtacac
    22021 cgattcgagc ggacagcgtg tcaaagagac gatctggttc cgtgtgtctg tgtggggcaa
    22081 gcaggccgaa agctgcaaca cctacttgaa gaagggtaag atggtgctgg tggaaggccg
    22141 cctggtctgt gatggagcta cgggcggtcc aaagattttc aagcggcaag acggaagctc
    22201 tggcgcgtcg tttgaaattt cggccagcac ggtgcgcttc ctgtcgccgc gcactgaggg
    22261 cgagttcgaa cccggcgaag ctccggcacc cgaagcgggc gatgaggata tccccttcta
    22321 aacagccatg acacacccca cccccccgcc cggtttagag ctgccccccg acctggagcc
    22381 gctctacagc aacctggcgc gcatcagcca caccccggct gagtttgtga ttgactttgc
    22441 gcgcttgctg cctggtcaaa ccaatctgaa cgtctcgacc cggctggtga tgtcgccagt
    22501 gagtctcaag ttgtttttcc gcgccctggc cgaaaacctg gcgcgctacg aagccagctt
    22561 tggcgaaatc aagctgcccg gcgatacgtc gctggcgacc gatcttttcc gtaatattca
    22621 acccccgccg cccccaccgg cggcggaatg aggctgcatg gaacccctgc aaattctcag
    22681 cgagcaagtg catcagattt ttgaaagccg caaccaggcc cgcgaccggg cactggccca
    22741 ggcccggcag ttgacgcgcc atgcggcgca gtcgattcga gctattcatc gcagtgaggt
    22801 tgagacggcg caccagcatc tgacagaagc ggcggcgata gtggccgcac tgcggcagga
    22861 actggcggct tttccagacc tgttctatgc aggttatacg caagacgcca tcaaggagtt
    22921 tgccgaggcc aatatcacct gcgcgctgat tgaaaaccgg gcgctgccga tgcctgaaga
    22981 cctgggcgtg gaatatgcca cctacctgaa cggcatggct gaagtgccgg gcgagctgcg
    23041 ccgccgctgc ctggatatcc tgcgcagtgg ttattcggcg gaggctgaac ggctgttggg
    23101 ctggatggac gagatttaca gtctgatgat tacggtggac tatcccgatg ccatcactaa
    23161 cggcctgcgg cgccagactg acctgctgcg cggcattgtg gaacgcaccc gcgcagacct
    23221 gacgttgagt cagcgggaag atcgactgca taacgccatg tcgtcgctgg cagaacaact
    23281 ggctgaccat gcgggcgatt cgagcctctg aactgggcag ttaccggttt tgtgagcgcg
    23341 cctggtggta tcaactgcag ggggttgaat cagataatct gaccgacctg gcagccggga
    23401 gcagttatca tcaatcgcat ggccggcgga cgctctccgc cggcctgatg cgcgccgccg
    23461 ggtggctgct gctgctgggc gggctggtga cgctggtagt ctggctgacc agccaggcga
    23521 ttaagtaggc gctgatgccg ctgttaatca tcttgctgct ggttggcgcc gtgctgctgc
    23581 tgtggctggg gggacggcag cgcaaggaaa gcgggctgcc aaccggccgg atcatctatg
    23641 cagacccggc cttatggggt gagactgaag cgcccctgta tgattccagc accgggctga
    23701 cgggccgccc cgattatctg gtgcggcagg gcgaagaaac gctgcccgtg gaagtgaaaa
    23761 gccgctatgc ccccgccgag ccttatgccg ggcatgtgct gcaactggcg gcttattgcc
    23821 ggttggttga ggcggagacc gggcggcgcc cgccctatgg gattctagtt taccggaacc
    23881 gcagctttgc gattgattac acacccgcgc tggaagccga gctgttggac ctgctgaaag
    23941 aagttcgtca gcaggaaatg cgcggggaac cagaccgctc tcacgaggag ccgggacggt
    24001 gcgagcgctg cgggtttcgc aaaacctgta accagcgcct gtaagttggt ataatcaaac
    24061 ttggcctatg tccaattcca gtcctcaacg cgttgctgtg atgatgcacg cttcactgcc
    24121 ggaagccgcc ggtgaagcag agcaaatctg ccagtttttg aaactgcgcg gacagacatg
    24181 cgtctgcgcg agtttttcag acgaggccat gcggcagcag gtgcaggctg gcgagtttga
    24241 cctggtgatt gcgctgggcg gggatggcac catgctgcga gccagccgcc tctgtgcgct
    24301 gccgggcacc ccagtcctgg gcattaacct ggggcatttt ggttttctaa ccgaggtgga
    24361 ccgcgctgag tggccgaccc gactggcgca attgctggag ggcaactatc ggctggagcg
    24421 ccgcatgatg ctgcgcgccg agcactggcg cggcgaggag atgctggcac actggctgct
    24481 gctcaatgag gtggtggtct gccgcggcgc aaccgtacga ccgattcggg tgagcgcgtg
    24541 ggtagacaat gaacccctga ccagctatgt ggctgacggg ctgattgcag catcggcgac
    24601 gggctccacc gcttatgctc tggctgccgg ggggcccgtg ctgccgcccg aactgcgcag
    24661 tattgtgatt gtgccggtag cgccgcacct ttcaatggac cgcgctattg tgctgcctga
    24721 aggcgcccag gtggtttttc aggtgcacgg acacagccca caggctgttt acagcggcga
    24781 cgggcaaacg ccagtgccgc tgcaagacgg ggatgccatt cacacccggg cggatgcgca
    24841 tacagcccag tttattcgtt ttcaggcccc gggcattttt tatcgcaacc tgaccatgta
    24901 tatggaacaa aacccgaccg caggattacc gcaatgaccc aggaaaccac tgacaattct
    24961 tacccgacgc tttattgtgc caatcaccca cagcgcccca ccctgctgcg gtgcaatcgc
    25021 tgtgaaaagc cgatttgcag cgactgcgct gtcaaaacac cggtgggtta ccgctgcaaa
    25081 gaatgtgtgc gcggtcagca aaaggtgtat gataccgcca agaaatggga ttatccggtg
    25141 gcctttttgg tagccgcgat cattgccttt ctgggcagtt ttaccactgc aattggattc
    25201 tttaccatct tcctcacccc gattatcggc gtgctggctg ccgaggcggt gcgctgggcg
    25261 acccgccggc ggcgcagtcc ggcgctgcac aagattgcgg cgctgtctgc ggggctggca
    25321 tcgctgccgc tggttatttt tcaggtcatc aacactgtcc tgatgctgtc tgatggctat
    25381 ggcctctggt atgcgattgg ccccatgatg tggcagggta tatatcttat tctgatcatc
    25441 agcactgttt actaccgact atctggcaaa ggctaaacga aagacaccat gctgaccgaa
    25501 ctgcgaatcg agaattttgc catcatccaa catctggagc tcagttttgg ctccgggctg
    25561 attgccttca cgggtgagac cggtgcgggc aaatccatta ttctggatgc ggttgaggcg
    25621 gtgattggcg gacgcgctga tgcggcgcaa attcgcagcg gcgccgaacg cgccagtgta
    25681 gaggcgatct tccggctgcc agaaacgagc cgggacgata ttgccgcctt gctggaagca
    25741 gaagatttgc tcgatgaccc caacgagatt attttggggc gcgaactgcg cgccaatggc
    25801 cgcagcattg cgcgggtgaa cgggcgcacc gtgaacctga gcctgctgcg cagcctgggc
    25861 agctatctgg tggatattca cgggcagtct gagcatcttt cactgctcaa tgtccgggcg
    25921 cacatgggat tgctggatcg ctttgccgag gcgagtgaag ccctggcggt ctaccggcag
    25981 acctaccagg ccgtgcgggc cgttcagcgc gagctggtgg gtctccaaac tcaagaggct
    26041 gatgtggcgc ggcgggtaga aatgctgacc tatcaggccg aagaaattga atctgcccgc
    26101 ctgcatgctg gcgaagaaga cgaactgcgc ctggaacgca accggctggc aaatgccgaa
    26161 aacctggcaa gcgcggctca gcaggcgctg acgctgctgg acgagggcag ccccgaagcc
    26221 gcagccatca ctgacttgct gggacagatg gtgcaactgc tgcacaccct ggaacgcgta
    26281 gacccaaccc aggccggact ggctgagcag gcggcgggat tatctgaaaa tgcagccgac
    26341 ctggcgctca gcctgcgggc ctatctggaa caaattgaag ccaacccccg ccgattggaa
    26401 gccctggaag accggctggc gctcatccac gatctgaagc gcaagtatgg cggcagcatt
    26461 gaaggcgccc tggcaagggc cgttgaggcc cgccagcagt tggaagccat cacccattcg
    26521 gcggagcgca ttgccgagct gcagagccag atgaccgacc tgcgcgcccg gctgaccgcc
    26581 caggcacaga ccctttcggc gctgcggcag caggcagccg tcaaactggg acaggcggtg
    26641 gaggttgagc tggctgattt aaatatgggc ggcgcacgct ttgccgtgga tttgcagacc
    26701 gagcccgacg cgaacggaat tgaagtgata ccgggcaaac cggtgagttt tgatgaaaat
    26761 ggggtggatc gctgtgaatt tttgattgcc cccaatcccg gcgagggctt gaagccgctg
    26821 gtcaagattg cctccggggg tgaaacctcc cggttgatgc tggccttgaa gaaggtgcta
    26881 accgaggctg atgctgtgcc cacgctcatc tttgatgaga ttgatcaggg tattggcggt
    26941 cgggtcggca tggtggtggg ggaaaagctg tggctgctgg ggcggcggca tcaggtatta
    27001 tgcgtgacac acctgccgca gttagccgcc tatggcgacc agcattactg cgtccgcaag
    27061 caggtggctg atggcagaac actgacacag gtggaagcgc tgaacgatgc gggacggcgg
    27121 gatgaactgg cgcagatgct gggcagcctg acggcagcca accgttcggc tgcgcaggaa
    27181 accctggctg tagcccgcca gcgggcactg gagttggccc ggcgggctca aggaagctga
    27241 gccgtggccc cggatggact gctacagata ttaatgctgg ggccgccagc cgtgcggcaa
    27301 aacgatcaac tgattgaaat tccgcgcaag caaacgcgcg ggctgctctt ttacctggcc
    27361 tgccagccgg ggccggtgac gcgcgacgaa ctggttgatt tgttctttga ggacgaagac
    27421 gaaatgtcgg cccggcggca cttgaccgaa gcgattacca agctgcgcgg agccttgttt
    27481 gcgccgatct atgtcgtcac caaaccagag acggtggaac tggacgcgcg gctgatctat
    27541 tcagatgtgg tggaatttag ccgcctgatt catgagagtg ggcgcccggg aggcagtggc
    27601 aaccgcccgc agcttccgac ggcaaccttt cagcggttga gccgggcggt ggatttgtgg
    27661 cggcagccgg cgcggtttct ctcgggagtg cgcctgccgg aaggcaataa tctggaaaaa
    27721 tggctgaaac aacttagcca ggaattagaa gttgattgga tgactgccat cacccaactg
    27781 gctgaccacc tggcagctaa cggggaatta caaaccgcca tagagcggct gcaattgggg
    27841 ctgaaccttg atccgactaa tgccgggtta aacattcgga agctgcgctg cctgattcag
    27901 agcggcaata tcaaacaggc agcggaatat gccaactttg tcgctgagca atatgatgat
    27961 gaaggtctct acctgccagg cgacctggcc gaagtgatgc agcaggtgct gaccttggaa
    28021 aatcagcccg tccccgaact gcaccccaac tgggaagggc ttgtgcaggc gcagaataag
    28081 tttgtggggc aggaagaagc cctggaagaa ctgaaacggg cctatcagcg cggcggggct
    28141 gcgctggtac tgggtgaagc aggttcgggt aagagccggc tggtttacga atttgtgcgc
    28201 cgccaaagcc cacagccgcg acagctgctg gcattgtgcc gcccgttgga agagaatctg
    28261 ccgctgcaac cctgggtgga tttgctgcgc cggcatgtac agccttcaga atggcagagc
    28321 attgaggccg cctggctcaa gccgctggcg cgcctgctgc ccgacctggc gattttgcgc
    28381 ccggaactca agccagagca agacgctgac aacgaggttc cccagacgca gttttttgag
    28441 gcactgcatc aggttctatt gaacctggcc agccgccagc gtgtggtgtt gtttgtggat
    28501 ggggcccagt ggggcgatga agcgacttat gcggcgctgg tctatctgct ggagcgggat
    28561 ttcttcaaca aaaacgggct gctgattctg gcggtccgcc cgcaggaaag caacccctca
    28621 ttggaaaagc tgcgcaacgc atccaatgcc ggacgcagcc tgacggccat tgatatgcag
    28681 cccctcaacc tggacgagat tgctcaggta gcccggtccg tccttgggca ggcgcccaca
    28741 cagcaactgg cctttcggct caagatggac agcgggggca atccgtatta tttattagag
    28801 ctgctctatg cactgcaaga cttgcatgtt gatatgaaca acccttacct gccagaagcg
    28861 ctgcccagtt ctggcagcct gattgcgtta attcagaccc gcacgcagca actgccgccc
    28921 cagtgccgcg aagtgctgga agccgcggcg gtgattggca aacgctttac gctggatcac
    28981 ttgcaggcgg tggcccggct gccgatggaa acgacagccg agacgctgac gatgctcatc
    29041 cgggcgcagt tgatccgtga gatgccagag gcgcgcccgg caggcggcta cgggtttgtg
    29101 catgatatca cccaggaggc aattcgacag agcctcttgc ggccgcgccg gcaggtactg
    29161 caccggcggc tagcgctggc attggaagcc agcgctcaac cgcagaccat caatctggaa
    29221 aaactggcct accactggga agaggccgga gatctgcaaa aggcctttaa ttactggttt
    29281 gaaaatggga aaaatgcaga aattaatgcc cgctggcagg aagccaagaa tgctttcaaa
    29341 cgggctgacc gcctgcggga acagttgggg gatgcgttgg ctgaccaaca gatttacctg
    29401 ctgtaccgct ggtgggcagg gacatttttc tacacaatgg agattgacca actgcggctg
    29461 accagccaaa aattatttca ggcaggcgaa cagcggagca gcgcgctgat gatgggctgc
    29521 gcgctgcgct gtatggcaga tgccgatgcg atggaacatc aaccagcgca gggattggtg
    29581 aatattgaac gcgccatgac gtacctggag ccgattcagg attattatga actggtggag
    29641 gcgcaaagct gccgagggtt gtgcctgtgg caactgggac gctacagcga atctctggac
    29701 gcctggactg ctgcggctgc attgagcgag aatcaagagc cgattggcac ccgcgcggcc
    29761 gggttgtttg cccggattcg gctggcgatt gcttttggcc ggctgggctg gccacgcaag
    29821 gctgaagccg aagcccgtaa gattattcaa gagagcctgg aactgcggcg ccccgttctg
    29881 gggttttatg ggcgcaatat tctggccggg gcgcaggacc gcatggggca gcattatatg
    29941 gcgctggaaa tcacccgttc ggtgctggca gcggcccggg aaagccataa tcccgggctg
    30001 gtgggaaata tcctctttgt gatggcagcc acccaaaccc aactgggcag gctggacgat
    30061 gcctgggaaa cgatcaccac cctgctgaat aatgtctctc aaattgagtt tgtcgatatt
    30121 ctcaagctgg ctaatttgct gcggggtgat attttccggc tgctggagtt ttacccggcc
    30181 gcggtgaaga attatcaaga tgggctgggg tctactttga gcggcattta cactcttgaa
    30241 aatctgcttg gcctgggaac aaccctgttg ctgcaaggtg agggggacct gggacatcat
    30301 tttctagaac gagtgattaa cgaggcgcag gctcaggaat tgaatatcta tcaaattgag
    30361 gctgaactgg cattaaccta tgcgcaaatc ctgaatgggg agattgaaac cgcagctcag
    30421 cggctggagc cgcttgagcg ccagttagcc gagcgtgagc taaccgtttt accctgcaac
    30481 cccgataagc tacacggatt gatggcactg cagcgagggg attatccggc agcgctggcg
    30541 ctggggcaag cctgcttaaa tcgggggctt gaacgccaaa atccctggct gcagatggaa
    30601 ggtttggagc tttgcctggc cgccgcgccc aaggaagaag cggcaagctg gcagcggcaa
    30661 attgaagcac tgctaaactg gctcgattct caaacccggc acgctgaact gcgccccgct
    30721 tttgctaact ttcaaaagcg agtgctggag cggattagtc ggagataatt gcctgcttgt
    30781 atttgtttca gaagtatgct agcatgaatc ggcgccgttg atctttgtcg tattgtaaat
    30841 tctccctgga gcgaaaaatg aataccaagc cttctactac caaccgcggg tgcgtggtgg
    30901 gggttgtttt attattcagc atgctgatct ggtctgtgct gcctattgat gttcgagcac
    30961 agcaaagtca gaccagcatc actacctatt taccattgat cctaaaacca tccggggggc
    31021 cacaatatgt gctggtggcc tggaatgatt taggaatgca ctgctacaac cgcgattttt
    31081 ccagtctggc tgttttgccg ccttataaca acctgtgggc acaggtgatc cggcgtggag
    31141 acccgccaca gattgtttcg gacaatatcc aggtcactta tttattcccg gataatactt
    31201 actccgttga gaaatctaat ttctgggact acgcccagga tctgttcaac ctgggaaccc
    31261 cattaacacc caatattggt ctcacaggtg taggaatggc aggcgagatg gcattcaacc
    31321 cgacgaataa tgcctttatt gtagaaggaa tacctctgac cgagttccgc gacagtgacc
    31381 catctacacc ttatccctat caattggcag agatcgccgc caaaaacgcg acgactggga
    31441 cagcgcttgc cagtttgacg gttgtagcgc ctgtatccac agaaatgacc tgcaatgact
    31501 gccatagtga taatgggcgc ggcaatgagg gtatatcaac cggtgatgtg gaaattaata
    31561 ttctaaccaa acatgatgat gaaaattccg aggattaccc ggttggtggt gaatatggta
    31621 acagcttagt tggaaatcaa cctgtgctat gcgccgattg ccattcatcg aatgcattgg
    31681 gcttgccagg agcgcctggg ctgccgagtt tatccaatgc aatgcacacc aaacatgctg
    31741 aaattgatcc gccgcagccc aattgctacc aatgccatcc cggacctacc acacaatgcc
    31801 tgcgggatgt catgtctact cagtatggaa tgacttgcga agattgtcac ggggccttgg
    31861 atgttgtggc gcaaaacccc gatccatggc tgcaggagcc gcgctgcgat ggttgccata
    31921 ccggttcggg atatgagcaa aacaatgcgc tataccgaca ctcgaccggg catggcggag
    31981 tttactgcga agcctgccac gacagcaccc acgccattgc tcccagcaat cagggcaatg
    32041 atggcattaa gttcacacaa ttacaaaatg cagccggacc tctgacaaaa tgcaccgtct
    32101 gccatctcac tgatcccggc gggacaaacc cgcacgatgt gataccttaa gagtacctgg
    32161 gctcaattaa gtaaataggc cagcagccag ttggtggttg ccagcagggc ttcggtatgg
    32221 gtgcgctcat aattgtgcga ggcgtccacg ccggggccga tcagcgccac ggccacatca
    32281 ccgccagagc gccagaatgc ggcgccatcg gagctgtaat aggggtagat gtcggcttta
    32341 tatgggatct gatactggtc tgccaggcgg cgcatgtgat tgctgagacc gtggtggtag
    32401 gggccgctgg agtctttgac acagatggag gcatggaatt cgtcagaagt ctggccttcg
    32461 ccaaccgcag ccatgtccac cgagatcaac tcagttactt cggcgggaac gcccgcggaa
    32521 gcgccgtgcc cgacctcttc gtaattgctg atgtgcaggt aagtggtctg gcgcggggtg
    32581 agacctgcgg cagaaatggc ggcaaccgcc gccacaatac aggccacgca ggccttgtca
    32641 tccaggtggc gggaacgcac gaagccgttg gtgatttcaa cgcgggggtc gaaagctaca
    32701 aaatcaccaa cctgaatacc cagggcccgg gtctgatcgg ctgaagatgt gatttcatcc
    32761 aggcgcactt ccatggcatc atcgccgcgc ttggcatcat tcacctcggc gccatagaca
    32821 tgggtggagg ctttggccag gagcaacgag ccgcgcactt tgcgcccgtc ggcggtgaag
    32881 atggtgcagc cctccccctc cactgtattc caggcatagc cgccaatttt ggtcagcttg
    32941 aggcggccgt tggatttgat ttctttgacc atggcgccca gggtatctac atgggccgtc
    33001 aggcagcggg gggagtcatc ggcctgaccg ggccagcggg ccaccagggc gcctttgcgg
    33061 gtgcgggtaa aggtcagagc ggggtaggcc tgtaaggccg cttcagtgaa gttgatgccg
    33121 gcatcggcaa aaccggtggg gctgggggtg ttcagcaagc cagcaagaaa agcaagcatc
    33181 tgttcagaat tgatgggggg gatcacggga aggctcctgg gtgaaagaga ttgggtgaat
    33241 taattcgccg gggcggctgc ctcgtaagcg gtgatgcgct cacgccagct ttgcggcagg
    33301 ggaatgctct gatgcgtatg atagtcgtag gcgaccatga cggtttcggc gcgggcatgc
    33361 agcagcccgg tagcttcatc tcgaatttca taggcgaagg tgaggctctt gttgcccagc
    33421 cggtcagcgc ggatatagac gcgcacggtt tgaccgagct caatgggggc caggtaagcc
    33481 atatgaatat cggcgacaat gaggcccagg ttgaagaagt ccacgccatc ccagaggccc
    33541 agttcgatga ggtaggcata gcgggcctgc tcaataaagc tgaggaagcg ggcgttgttg
    33601 acgtgccact gggggtccag gtcactgtag cggatgggga ttgggtagga gaagttgaat
    33661 tgggtcatgg ctccattata ctcaaaaacc ggccgcggca atccttgtca agggtggcgc
    33721 catctgctat aatcaggtcg ttcgggcgcg tagctcagtt ggttagagcg cacggttcac
    33781 atccgtgagg ttcgtaggtt cgagccctat cgcgcccacc tcaatgggcc ttaattgccc
    33841 gccctcactc ccccgcaaag gctcatcctt tgtggggttt gtgttttacc cgaaaaagat
    33901 tatctactga tggcctgatt tctggatgcc caccgaatcg cccacccctc tcattgaatt
    33961 acgtcaggtc gtcaaagttt accagacggc tgccggggaa tttccagccc tgcgggggat
    34021 tgatgcgagc atttatgccg gcgaatttct ggggattatt ggcaaatctg gggcgggaaa
    34081 atccaccctg ctcaacatgc tcactggtgt cgatcatctt actgccggcg aagtggttgt
    34141 gtggggcaat ggagagcccg ttgccatcca caaattatct gagaacgatc gggcgctctg
    34201 gcgaggacgg accatggggg tggtatacca gtcttttcaa ctgctgccca tgctcacatt
    34261 ggtagataat atcttgctgc caatggattt gtgcggcctg tataagcccg gtgcaagccg
    34321 ggcgcgggcc tacgaactgc tgcgcatggt agagttggaa gagcacgcca ccaaactgcc
    34381 cgattttatc tctggcgggc agcagcagcg cgtggccatt gcacgcgccc tggcaaacga
    34441 cccacccatt ctggtcgctg atgaacccac cggcagtctg gattcggtta cggcagacca
    34501 tgtatttcaa gtctttgagc agttagtctc ccagggcaaa accatcatta tggcgactca
    34561 tgacgccagt ttgatgccgc gtttttcacg tcacctgtat attcaggatg gccaaatcgt
    34621 ccctgacccc actccagcgg atccacctgc cacccttatg gcagcacccc accggaggaa
    34681 gcgatgatac cggcgcaggt ttcaacccaa acaggcgaaa cagccattca acttcagaag
    34741 gtagtaaaaa ccttcaagaa tgctgcgggg gagtttactg tgctgaaagg gatcgacctg
    34801 aacctgcatc gcggcgagtt cgtttcaatt gtggggaaat cgggcagtgg caaatcgacc
    34861 ttgttgaaca tgatcaccgg cattgaccac cctacgggag gacaggtcat catcaacggg
    34921 gtgaatatct atacgctgaa tgagagccag cgagctctct ggcgcggtaa aaacctcggg
    34981 attgtcttcc aatttttcca acttttgccc atgctgacct tgctggaaaa tgtgatgctg
    35041 ccaatggatt atgccggtct gtatgatttc gatgagcgcc caaaacgcgc cctggggtta
    35101 ctccaactgg ttggtttaga gggacaggcg cacaaactcc ctgcggctgt ttccacgggg
    35161 cagcagcaaa gtgcagctat tgcgcgcgcc ctggctaccg acccgcccat catctgtgcg
    35221 gatgagccaa ccggtaactt agactcccgc tcggctgaag ccatcattgg attgtttgaa
    35281 cgcctggtgg aggccgggaa aaccattgcc atggttaccc acgatccgac attgacgcgg
    35341 cgtacaaccc gcacaatcat catctcagac ggcgagctga ttgatgaaac cgtggcccag
    35401 gcactgcctc agttgggaca ccgccagatg cttgaagcct cgcgccagtt ggagcgtttt
    35461 acctgccagc ccaacagccc gatcttacag caaggccagc atgtggatta tttctatatg
    35521 attgtgagcg gcagcgtgga agtggtttta tgcaaccgcc ggcaagagta cacggtagcg
    35581 cggctggggc cggggcaatt ttttggcgag gtagaattga tacatggagg ccaatcgata
    35641 gcctgtgtga gggcagtgcc cgatgggccg gttacgctgg cagcactttc gcgcgacaaa
    35701 tttaaccaat tgattaaaag ctcacccctg accgaagaag ccctggccca ggtggtgcag
    35761 ctccgactgg cagagaaccg cacgggagac cggcggcaaa ggcagagata agaaatggag
    35821 aacaccctat gcgtccacgc tggcgcaaag tactgcacga tctgattgat aatatttcgc
    35881 gcaccacgct ggttgttctt tcaattgtgg tgggtgtgtt ttccattggc gtcatcattg
    35941 gggcctatgt catcatttca aatgatatga gcgccagtta tgcggccaac catcccatga
    36001 acatagaact gcgctgcggt gcttttgatg acgatctgct ctcgatggtg aagcacatga
    36061 acggcgtaga agcggctgaa gggcgcagct ttttcaccat gcgggtgcgt ctggctggcg
    36121 gagaacaatg ggaaacgctc aatattgtgg gcgttgatga ttttgaaaaa ctggatatca
    36181 atcggttaaa tatcatcagc gggacgaacc tgccagcaga tgagcaggtg ctgctggaaa
    36241 agaaaacact ggataatctg gatgtggttg tgggcgacac gcttgagttt gagctgctgg
    36301 atggaagcat caaaaccctg caagtggttg ggattgttca agaccccgct accggcgcag
    36361 gtgattttct cgcgcagcca tttgcctacg tcacgactgg cacactgccg tttctgggac
    36421 aaactgaaag ctttaaccgc atgtttgtca cggtgacgga gggacagaat gatctggaac
    36481 atttggatga gatgtctgca gctattcgag ataagattga aaaaagcggc atcggggtct
    36541 atacctcgcg gacaacaaag acaaacgaac accccatggc ctcaaccgtg caggctgttt
    36601 tgggtatttt gctggcgctg ggggtattaa ttttattcct cagtagttcg ttaattgcca
    36661 atacgctcag cgctttgctt aatcaacacc tgcggcatat tggcgtgatg aaactggtgg
    36721 gtgcgcgccg cggtcagatt ctgcaaatgt actttgctct gatcctggct tttggtgtga
    36781 ttgcactggt gatcgccgtc ccattgggtg ggcaaggcgc ttatagcctg gctaacttta
    36841 tagccagtaa gataaacttt aacctgctgg gataccgtat tgtgccattt tccctgctgg
    36901 tgcagattgt tgttgggctg gcggtgccct tgctggcggg attactaccc gtactgagcg
    36961 gctcgcgaac cactgttctg caagcgctga ccggcagcat caatgaggat agccattctc
    37021 aggtggcggg cagtctgcca gaagaggctg gttttgagcg gcttgaggcc cgggtggcgc
    37081 atacattggg tcgccagagc atccatgttc cccggccatt gctgctttca ctgcgcaata
    37141 ctttccggcg taaagggcgg ttggcgctga cacttttcac gctgacgatg gggggggcca
    37201 ttttcatcgc agttttcaat gtacaggtaa ccctccacga ctatattgac cagattggca
    37261 aatattttct ggccgatgtt accctggatt ttgatcgtta ttaccggctg aacgatgtta
    37321 aaacgactgc catgaacatc ccaggggtgg ttgatgtgga agggtgggct tatgccagtg
    37381 cagaagtcct ctaccccaat gaccgggtgg cgcgaaatat caccattctg gcacccccgg
    37441 ccagcagcca actggtatcg ccgctgctcg tggaagggcg ttggattcaa ccgggagatg
    37501 agaaagttat caccattagt gaggctattc tggaggagtt tccagacctt cacgccgggc
    37561 agactttacg actcaacgtc aatggacgca aagatgactg gctggttgtg ggcattttta
    37621 aattcgtgga tcagcagggg acatttgcct atagcaccta tgaatacatc tcacatcaag
    37681 cgcatatggc gaaccgcgcc ttttcatatc gcatcgtaac cgttcagcat gacccggcct
    37741 atcagcaaaa gatgggtgaa attctggatg cagccttccg cgcccgcggg ttccatgtca
    37801 gtcaggcgcg caccgggcaa tctacactgg cactggcagc agaaagcctg gatatcctgg
    37861 tcactttcct gctgattatg gccttgctga cggcactggt gggcagcatt ggcctggcgg
    37921 ggacgatggg gatgaatgtg atggaacgga cgcgcgaaat tggcgtgatg cgttccattg
    37981 gcgcggcaga caaagaaatt atgcgcagcg tgatcatcga agggatggtg attggtatga
    38041 tttcctggct gctcggcgcg ctgctttcat tcccatttac ttatctgctc acatacatta
    38101 ttagcgtggc tatttttcac tcaccgattg ctgtgcattt cacagccacc ggttttggtg
    38161 tgtggctgct ggttgtgctg gtcttatcgg cggtagccag cgcattgcca gctcgtaacg
    38221 ccgcccgcct gaccattcgt gaagtactgg cttacgagta attcgtcaat tgaaggagaa
    38281 tcccatgaag aaagtcccga tgattgtgat cctgattctg gtcatgctat tgaccgcctg
    38341 cggaaaagcg accgaggctg cgcccattgc caatcctgcg cctgcagttg atgtggtgat
    38401 tgcggaagga catttaattc ccaatcagag ccagtatttg gtttttccag ttcgcgggcg
    38461 tgtggcagaa attctggtaa gtgaaggaga cagcgtcgac atggggcaag ttttgatccg
    38521 gctgggggac agcgagcaag cccaggcggg gcttgcggct gcgcagttgg agctgaccca
    38581 ggcgcagcaa gattatgatg cgctgatccg cacagccgat cttgggcatg cccaggcctg
    38641 gcaagcctat attgatgcgc agaaagtgcg tgcggctgcc caattagcct gggatcggct
    38701 ggatctgagt gccattgaga ttgagattga taacgcccag gcggaggtca cttcccggca
    38761 aaccgattta gacgaggcgc agaccgactt cgataaatat cgcttcctgc ctgtggaaaa
    38821 tgtcacccgc caaacctatg aggacagcct gcgcacagcc cagatagatt atgatacagc
    38881 cgtgcagaaa cgggaagacc tggctcatca gcgcgatgcc gttcaggcaa cattggatgc
    38941 agctcgcgcc accgaagccg aagctctgcg cgactacgat aacacggtct ctggcgcgga
    39001 tgttgatcaa ctggcgctgg cacaggcccg gcttgatgcc gcccaggcgc aggtcagtgc
    39061 agcacaggct gcactggata attatgagct caaagccccc ttcaatggaa ccatagcaga
    39121 cattgatatt cgggtgggcg aatgggccgg gccagaagcg tgggtggcag ccctggtgga
    39181 caccagcgcc tggtatgtag aaaccagtga cctctctgaa ctggatatgg tagagatcgg
    39241 cgtgggacaa tcggttgagg tgactgccga cgcccttccc gatgtggtca tgacgggggt
    39301 tgtggaatcg atcagcgcag cgccccggat acagggcagc gacatcttat atacggtgca
    39361 gattcgcatg gacgaggtgg accccagcct gcgttggggt atgacaatgg aagtgacctt
    39421 tacgccgcaa gaggcaaacc gctgattcgc tgagttaact tgacagaccg gattgtgccg
    39481 ggatagaatg aggatgttcc cttctcataa tattcttaag gagttttcaa atgactgagt
    39541 tttcacgtat gccgcgctat gatatccgca atgacggcgc ggggccatac gccatcttct
    39601 actgtgatat ctgcgggcgc gagtttcgca gccagccgga tgtggtgggc accatatcat
    39661 ccgacattgg ccgccaggct gccagcggcc tgctgcgcaa gattccgctg gtgggcagtg
    39721 cggtagccaa caaggttacc ggggaagatc cccgctacag ctacaacctg accccggcgc
    39781 agctggaatc agcctggaag caggtgcagc agcattttcg ccagtgcccc acctgcaccc
    39841 ggatcgtctg cctgtcggac tttgatacgc agtcgggcta ctgccaggaa gacagcccgc
    39901 gcaccgatga gattgccgag tcgcggggtc agcaagccgg cgcggcgctg aagggttttg
    39961 ccgcggcgct ggggttggat cgcgttgcca gccaggtttc aaacgcggct gaggccgctc
    40021 agcgagcgac cagcgccatg gcacgctgcc ccaaagatgg gaccctggca gcaccgggaa
    40081 ccaagttctg cccggaatgt gggactgcca tggttcaacc tgcggcagca gcctgcccca
    40141 agtgcggagc cgacgtacac ggcgccaagt tctgcccgga atgcggcacc aagattgagg
    40201 ctgctccccg cccggccaac tgcccgcaat gcggcaccgc cacagaaggg gccaaattct
    40261 gccccaactg cggcgccaag caggggtaac ctgccagcat tttaaaacct cactgcgccc
    40321 tccgagtgga gggcgcagcg tttttgatca agatgagcca aattggatct gacagtataa
    40381 tgatgtcaag gagatgtttg ccatgacccc gattgttcgc ccctgtgaat tgaaggattt
    40441 gcccgctgtg ctgggactgc tgacccagct tgaagaagtg gcgcacgccc aatcagattt
    40501 gaaactggaa cggctgcgcg aactgtttgt tgagatggca gaatgcccgg aaatctacct
    40561 caacttggtc tgcgaggcgg atggtcaggt ggcgggtttt ctgtcgatga ttttttaccg
    40621 gacgtttttt catcggggcg gcaccgcgct ggtgaatgaa ctggtggttg acgagaagcg
    40681 gcgcggattg gggctgggtc aactgctgat gcaaaccgcc atcaccgagg cgctggcgcg
    40741 cgatatggat gagattgagg tgggcacaga agaagataac caggccgccc aggcctttta
    40801 cagccgggcg ggcctgaatg agcggtatgt actgctggga atggaatttg aaaccgaaga
    40861 atagttagcg ggtttcggtc actttgcgga tggtgcgggg ctgctccgta tcgtagccct
    40921 tggcgcgggt caggtgatag gcaaacaact gcccggcagg aatgctgaca atcggcgtga
    40981 gggattcagg ggttgctgcc ggaatacgaa tggccgactt gcccatggca agggccgatt
    41041 cgtcgttgga gatgatgacc agttctgcca gcagatcatc tttgaggtgg tgcagcagtt
    41101 ccatttgtgg ggcataaacc tgcccggtag gggcaacggc catcaccggg aagccgcgct
    41161 caaccatggc aatggggccg tgcaaaaagt cggcggatga atagggctcg gcagagacgt
    41221 aggtgagttc tttgagcttg agtgcccact caaaggcggt ggcatagttg aagccgcgcc
    41281 ccagcaccac gcactgctgc atgtagcggt aacgctcagc cgcggttgcg atggagctat
    41341 cctgctggag ggtttgcgcc acccaatcgg gcacctgggc aagggcttcc caggcggcag
    41401 cgtcatcggc gagggcagca gagagcatcg ccagagccag cagttcggcg gtgtaagttt
    41461 tggtggcggc gacagcccgc tcggggcccg cgcagatgtc cagcacataa tcggcggctt
    41521 ttgccagcgg ggaatcggcg caattggtaa tagccagcgt ggggcagccc tggcggcggc
    41581 cttcttccag cacgccgaca atatcgggtg attgaccgga ctgggagata cccagcacca
    41641 gggcattctt gaggcgcggc ggctgatggt agaaggtgaa cagcgacggg gttgccagcg
    41701 ccacgggcag ttgattggcc gcgccccaga gatagttggc ataacgccca gcattatcgg
    41761 aggtgccgcg ggcagccagg aagacgtaat ccacactgcg ctgacgcagg tcacgggcaa
    41821 tcgccagcac ggcatcacgc tgggtggtga gcaggcgagt caaaacagcg ggttgttcaa
    41881 agatttcggc atacaggctc atgagggttt ggcttctttc ggttgaggat taagcgcgca
    41941 ggcgggcaat gcgggccgcg acgtcgctct ggaagcggtc gatagcggcg cgaataaaat
    42001 cagcggggtc gaggtcaggg tcagctacca ggggggtcag atccatggcg aaggccaggc
    42061 cggtggctgt atctacgccg tgagaggcgt aataggtggc gttggcgcga cggcgggcca
    42121 gggtggacag gtcatagcgc ttgcccccgc tgatctgcga atcaaagacg ccaagcaagg
    42181 cagactggag gttttcctgg tcggagagat caaaggcgat cttctcctcg tccagcatcc
    42241 agtccaggcc acgccagacc tcacaaccaa agacctgctg cgggcgctcg gcgggcggca
    42301 ggcggcgcag ggcggcaatg gctttaagcg ccacgccaac gtgggtatcg tgcttgtcgg
    42361 cgaggttgtg ggtatagaca acctgcgggc gggctgcctt cagcaaggcc accagatctt
    42421 ccacggggcg atcatcggcg cccgatttga cggcagagct gggataatcc aggatcacct
    42481 gggcggcata ctcgcccaca caggcggctt tacgctgctc cttgaagcgc acgaggcgca
    42541 tttcttcatt gtcatattgg gcataaagcc cggcacgggg tgagccgcgg ccatccgtga
    42601 ctaccacgcc agtgaaccag cgatctgtgc gctgaaaaca ttccagaatg ggtgcggcgg
    42661 ccataatctc gatgtcatcc tgatgggcgg cgatggccag atgtgtggtg cgcgccaggg
    42721 cggcggccgc gggctggccg tcggggatgt aaagatcagc agtatccaga tggaatttca
    42781 tgccaggctc ctaaggtttg atttccggca ggctggccgc ggcaatggac tgccccacgc
    42841 ggcggctttt ctcgtcgggt aattgaattt ggaaagcgcc agccagatcg ggaaactcgg
    42901 cagccaggac ctgccgggcg gtctgaagga taatttcgcc gccgcgaccc gaggtgcagc
    42961 gccccaggat cagcacatgg cgcatggtgt aaaaatcagc atagtgggcc agggcataac
    43021 ccaggtaaat gcccatgctc tgccagatct gcaccgcgcc ggggtgatcg gcttcaagca
    43081 gcgcctgaac cgtctgcagc ttctcggcat cggtgatatt ggcggggatg tggatgccag
    43141 cctgcggcgc cagccggaag acgcactgct gcgagaaata ctgcgagccg cagccagcat
    43201 cgccggacca ttcttccact ggagcggtgg gggcataatc taccggggca aatgaaagct
    43261 cattcaacca gccaaggata tgcccttcga catcaacata gccggctgcc tcgctggagc
    43321 ccatggcaat gcccagaata ccattctctt caagcgacat ggcgcccgcc agggcggtca
    43381 catcgccatc gttgatgaca taaatcggca cgcccagctc cgcagagagg cgcacaaaca
    43441 gggtgcggat ctgatcgtac tggtcgggcg gcacagcgcg gaaaagcgag gcgaccatgg
    43501 ggcggttatc tatgtagatg cccgccgaac tgccgccaat ggcgtccaca cggggcaggt
    43561 gcgccgcggc tgagcggatg gcagtcagaa tctcgcggta atgataggcc gggtcagcgt
    43621 gatttttggg ttcccagatc acctcttcgc tgaagaccac gcggccatcc agcaccgcgc
    43681 tgaccttgcg gtctgaggcg cccaggtcaa aaccgatgcg gcagccgttg aggtggcggc
    43741 caatggcctg cccctgctcg cgggcggggg gaacatcctc ggggcggcag gcaaccacct
    43801 caaaggggtg ctgatagacc tgactgccca taaaatccac atcaaagcgg cgcgggccgg
    43861 tgagggcgta aatggcacgc agttgagcgg ccagttcagc cgggccgccc acgtagaggc
    43921 gaaatccgcc gcgctgccag agcaggaatt tgaccagccg ctcgatgtag ggcaggtttt
    43981 cgccggcctc cgggtgaccg ggggggaaga tggcggtttc gcagcgcgag atgcggccat
    44041 cttcacgttc gaggcccagc accaccggta cgccggcggc gccaacacgg cggcggtaat
    44101 gccggttggc caggctggcc gggcgaaagt tggggtcaag cgggggaaga atggcgggag
    44161 ggacgagagt taatgaagcc atgcagcaca atccttaaac ggtcagaact gcatggattg
    44221 tacttcaaaa cccggggcgc ggtcaaaaca tccgggtacc ggtggggaaa attttacatc
    44281 tatctggata acaggccaaa cacagaggcg ttaacgacac acgttactct gccacccgtt
    44341 cagcagtcag gtcttcgccg ttttggcgga aggccagggc ggtgatgcgg ccgtcttcac
    44401 gctggaattg gatggaggcg ttgagctggg tgaggtaaaa gcggtccaca tcccgcgggc
    44461 aaagcgcaag cgcgggctga ccggggattt cgagggtgag gccggcggga gtatgacgga
    44521 tgtggaactg ccagtcgcca gaagcataat cgccggcgaa ttcggccagg gcggcgttgg
    44581 cgggtggatt gggcatgggc aggaaggggg tatctacaaa gctgtcccag ccaaattcca
    44641 gggccacgtt gttgatccat tcatcggtca gggcgtcgcc gctgtcagag ttggtcatga
    44701 tgattgcagc ctgcccccgc cccttacggg ctagaatctg gttgcggtag ccttcgttgg
    44761 atccgccgtg accgtaatac agatcatccg gttcgccggt gaggaagaag cccaggccct
    44821 catcgccatc cagaccaatg ccgcgcagcg gaatctgcgg ggtaagcata tcagccagca
    44881 ggtcggggcg caccaggcgg tcggtttcgc cgcgctgggt ggcttccagc aggacaatca
    44941 ggcgggcgag atcagtgggc gtggtccata gaccagcggc ggcactttcg ggatagcggt
    45001 gccagttgcc aatcagcggg tcgccgttat tgcggtgccc cacagcagcg cgggagtgcc
    45061 agtcggcagg cagggggtcc aggtaggtag agtgcgtcat cccggcgggc tccagcacgc
    45121 gctggcgggc ggcctgccag aacggctgac cggtgaggtc ttcgatgagc tgctgcacaa
    45181 tggtatagcc gccgccggaa tagcgaaaat ggaaaccggg gagggtatcc acccgaatgg
    45241 ccggggagtt ggcgggcggc tcgccgttca aaatttgctg gagcgtgggc acggcagaac
    45301 gggccggata gcccaggaag ccatccacgg tgatgccgcc ggagtgtgag agcagttggc
    45361 gcagggtgac gcgcggctgc cagccagctc gggcaggaat ctgccaggtg cgcagaaagt
    45421 ctcgcaggtc ggcatcgagg gacaggcggc cttcagccac caggctgagg gccgtgagcg
    45481 cggccaccgg cttgctgatg gaagccgcct ggaagcaggt ctccggggtg accggatcgg
    45541 gatcgccctg gcgccggacg ccataggccc ccgcccagat gatctcgccg ccttcgacaa
    45601 tggccatgct ggcgccgggg ataaaccgcg cggccatgcg ctcggtcagc ggcagttcac
    45661 gccagccatc ggcatagaat gggtgaaagg tgcggaaacg ctgctcaata cgcgccagcg
    45721 aggcttggta acttttgggc attggagaac tccagttggg gggatggcta aattataaac
    45781 cgaacagaca gggttttaag gcttaatttt aaattttcaa aatggttgta gattgatacc
    45841 aaggaaaagg ggtgcgcctg tcatttaatg cgggcttaaa atcctgacgg aacaaaagcc
    45901 gtgttccggc ttgcttgact ttcaatgctc accctgatag gattgacgcg attttccatt
    45961 tgcaggagat tgtatcttga gcactacccc gcccgctgtt gtgccgtttg aatcacagcc
    46021 gcctcaggtg atgaaacgga atttcttcct gggcgccatt aacggcgttt tatttgtatt
    46081 tgcagaaacc ctgttcgatc ccaccctggt ggtcacctca ctggccaatc aactgggggc
    46141 ttcggcgttc attctgggtt tgtttggacc catcagcagt gcagggtggt atctgccgcc
    46201 gttctttgtg gcggggtatg tccaaagccg cccattgaaa atagcggttt accgggggat
    46261 gtcgttcatc cgaatcgcct gctggatttt gctggcagcc gtggtcaatc tggtgcgaga
    46321 cccggcctgg attttgattt ctttaattgt cgcttacagc attgcctcgc tggcctcagg
    46381 gatcggcggg ctgccgtttc tagaagtggt gagcaagacc gtgccgcccg tccgccgcgg
    46441 cgagttgttt gcctggcggt tggggctggg cggactgctg ggcatcaccg cgagcgggct
    46501 ggtgcgctgg ctgctggatc cagcaacccc gattacattc cccaataatt acggcctgct
    46561 ggcaattgga ttctgcctgt ttgcatcgat ctcgctgatg ctgttcaacc agatcaaaga
    46621 gacgccagac gcaaacccgc ggccctcccg gccttttcgg cagcaactgc gcattgccct
    46681 ggggattgtg cgcaccagcc cgaactaccg acggcacctg ctgacacagg ccctgttgtt
    46741 tgtgggcgtg tctgccgtaa cgttttatat ggtgtatgtg aaccgggaac tggatgcacc
    46801 gctgcaatgg gtgggcgtct acctggcggt gcaaatggtg gccaacttgc tggccaacct
    46861 gctgttcggc tggctctcac ggcgggtggg gaaccagaaa gtgctgactg tgggcgcggc
    46921 tgctggtgtt attgccgcag ccatcatgac cgggatggtg ctgctggcaa agccgctggc
    46981 tatttcggga ctgactgcgg cctggctgct gacgccggta tttgccatgg tgggcattcg
    47041 ggtggcggcg gtgggcgtag cctcgcccag cctgatgctc aatattgtgc caccggaaga
    47101 gcgctcgctg atgattggtt ttacccagac agtgggcggg atggtggtgc tgctgaccat
    47161 gaccagcggc ctgattgtgg gcgcggtggg ttatccggcc ttgatgatca tttcactggc
    47221 ggcctatatt ggcgccgtgc tgctggcact gcaaattcgc gagcaccctg atctgtgata
    47281 agttttatgg tatcattcgg cattccctga ccgccatcca acaaaggtta tccaatatga
    47341 acacaaccgc ccaaacatcc cgcaaatacc tgtggattta tatcctgatt gcctttggtt
    47401 ttacctgggc actgtggatt cccacgctgc tgctcagcga gcaaaagagc tacacactgc
    47461 cgctggcggc gaattatccg gcgttgtttg aagaggggtt tgtcaattcc gaacatattt
    47521 tgctggcggt cttgttcagc ctggcggttt atggaccgct gctggccgcc accatcacgg
    47581 cggggctgga gggcggcaag agcggcgtgc aggcgctgtt tgcatccttc aaacgctggg
    47641 gcagcggcaa gtggtatggg cgggcggcgc tggtcctgat tgccgttgca ggagtgccca
    47701 tgttgattgg gctgctgacg ggtctgacaa aacccaacgg gacagcgctg ctggcgtttt
    47761 tgccgtggct gctgccgatc tttatcatgc aaattctcac cagcgggttg ggggaagaac
    47821 ccggctggcg cggctatttg ctgccccggc tgcagcaaac cctcagcggc gagaagccgg
    47881 tctgggtgct gggaatcatc tgggcggtgt ggcattttcc gttcaccatc tattccacat
    47941 tagccaccac ggttgaaatg cccgccgcgg cgctggttac caccatcatc atgagtttgg
    48001 cggggcaaac catctcttta attggcgtca cttacctgta tgtctggatt tataaccaca
    48061 cccgcagtct gccgctggtg atcttcttcc acgcgctaag caataccctg cccctgattc
    48121 cacttggcgg atggagcccg ctggtgagcc tgctgacggc agcaatgccc tgggtgattg
    48181 tctttgtgct ggagaaaaaa ctgcccaaag gtgagtttcc gggacagccg gtgcaataag
    48241 ctttactcag ccgggtaagt gcggcgcacc tcacgcaaaa actgcggggt gttcaatcga
    48301 cgttccagca ggtatgtcag atactgctgg agcaggctct ccatctcccg gcggaccggc
    48361 tcggaggggt cggcgcgggc ggcttcggca taggtggagc gctgcagatg gcgcaggtat
    48421 ttgagagccg ccattgaaat gggccaggcc tgcgcgcccg gattgaggcc gcagcgcggg
    48481 cagagcgctc cgccctgcag ggcagagaag aactgatcct ggggttgaat cagagcgccg
    48541 cagcccacgc agtggaacag ctccggacgg aagccggaca ggtcaaggag gcggatatcg
    48601 taatagcgca ccgccaggaa gggatcgggc agggcggcaa tgcgcgccag ggtatcggtc
    48661 aacaggttat agagggggcg attttcgccc tcctcatagg taaagcggtc taaaagttca
    48721 atcacataag aggcgtagcc gatgcgggtc aggtcctgac cgatgggcag ataggcatcc
    48781 accgtctcag cctgggtgac aatccaggca tcacggccgc gcgccagcat gagcgccaca
    48841 cgggtgaagg gctccaggtg cccggccttt cgggagcgca ccttgcgggc acccttggca
    48901 acggcgcgca gcttgccagc ctcgcgcgtg aagagggtga gcaggcgatc cgcctcgccc
    48961 caatccagat ggcgtaatac cacggcttca actcgtagag aacgctgctc ttttggcatg
    49021 agtctattta accacaattc ggcaagtttt tgttacaata aaatcaaact tctctccgga
    49081 gcgataataa tgtacttgca acccgaagac ctgttgaaaa ttgtgctggc tgtcctggtc
    49141 gggggattga ttgggattga gcgcgaatat cgtgataagg cggctggatt tcggacgctg
    49201 atttttatct gcgtgggagc cacattattc accatcctgt ctgtgcggtt ggcgggagat
    49261 aatgacccga cccggattgc cgcaagcatt gtgacgggag tggggttcct gggcgccggt
    49321 gtgatcctgc gggacggcgg gcgcgtgatt gggctgacga cggccgcggc catctggctg
    49381 acggcggcgc tgggggtggg cctgggcggc ggactttatg cgctggtggg cgtctcaacg
    49441 gtgctggcgc tgattgtgct gtgggtgctg cccttttttg aacaccgcct ggataacctt
    49501 tgggaagagc gcacttatga aattgtctgt gcgctggcac cagagaaggt ggctgaactg
    49561 gagcacctgt ttgcacagaa taagctgcgc gtcaaaaccc accgcctgac ccgttctggc
    49621 ggcgagatga cccttaacct gctggtgcgg ggcccgctaa aaggccatga gaaggtcctg
    49681 gagcagattt ttgcccgaga tgatgtgcac acctgccatt actagaacag gcggcgctga
    49741 tgattgattg gattatcatc ggcgcggccg ggctgctggt ggtcagcgtg ctggcaagcc
    49801 gtatttctga tcggtttggg gtgccggtgc tgctcatctt cctggcgctg gggatgctgt
    49861 ccgggtctga tgggattggg ggcatctatt tcgataatcc acagattgcc caatcggtag
    49921 gggtggtggc gctggtgctg atcctgtttt cgggcggttt ggataccgaa tggcgcagcg
    49981 tccggccaat cgtcaaagag gggctgctgc tttcaactgt gggcgtgctt ttgaccgcgc
    50041 tgggtgtggg gttgattgcc gccctgctgc tcaagctctc aatcgtggaa gggctgctgc
    50101 tgggtgcggt gatctcatcc accgatgcgg cggcggtctt ttcggtgctg cgcgccaagg
    50161 gcatcaacct gaaggggcag ctcaagccac tgctggaatt tgaatcgggc agcaatgacc
    50221 cgatggcaat tttcctgacg gtgggattga ttcaaattct gacacagcct gatttctcaa
    50281 cgctggatct gctgggctcg tttgtgctgc aaatgctgct gggggccggg ctgggggtgg
    50341 cggctggcta tgggatgctt ttcctgctca accggattcg cctgggctat gacgggcttt
    50401 acccggtctt caccacggcg ctggtttttc tgaccttcgg gctgaccaac gtcgtcggtg
    50461 ggaacgggtt tttagccgtg tacctggccg ggattatgct ccggcggggc gatttcatcc
    50521 accgcaaaag tgtaatgcgc ttccacgacg ggctggcctg gttgatgcaa atcaccatgt
    50581 tcctgacgct gggcctgctg gttttcccct cccggctgcc gccaatcatg ggtgttggcc
    50641 tgctgattgc cattgggttg atgtttgtgg cacggccgat tggcgttttt ctggccttgc
    50701 tgccgagcaa actcaagatc cgtgaaaaag cgtttatttc ctgggtgggg ctgcgtgggg
    50761 ctgtgccgat catcctggca acttaccccc tgctggccgg gctggagcag gccgagctga
    50821 tgtttaatgt cgtcttcttt gtggtactga cctccaccct gttccagggc acactgattc
    50881 cgcaggtggc ccgctggttg aaagtggatg cgccgctgga ggcaaaacgg gtctatccac
    50941 tggaatacac ccgcatggaa gggctgagca gcgaattgaa agaatatccc attccggcgg
    51001 attcgtttgt ggtggggaag tctattgtgg agttggggct gccgcagcaa tttttgatta
    51061 tgctgattgc ccgaggcaac gaatttgtgc tgccgagcgg cggcacggtg ctggaagccg
    51121 gcgatgtgct gctggccctg accgatgctg aatcaagcga gattgtgcgc gagcaaattg
    51181 cgattaagac ggcttcttga gcccgctttt tttatgatag cctgataaaa taaccttatg
    51241 tccaccgcgt ctgtcccgct ggcgttcctg ttcacggata ttgaaggctc tacccgtttg
    51301 tgggaggctc acccagatgc aatgcaggct gcactggcct gccacgatgc ccgcttgcgg
    51361 gcagtgattg ccgctcacgg gggtgaagtt tttaagacca ttggtgatgc tttctgcgtc
    51421 accttctctt cgccattgga ggccgttcag gcggcgctgg aagcccagcg agagctgacc
    51481 gcagagccct gcggcgcgct ggaactgcgg gtgcggatgg cggtgcacag tggaactgcc
    51541 gaaagccggg atggcgatta ttttgggcag ccactcaacc gggtggcgcg cctgctggca
    51601 gccgggcacg gcgggcaggt gctggtctcg caggctgtcc acgaacaggt gaaagatcaa
    51661 ctgccacctg aggtctgttt attggacctt ggcgagcggc gcctgaaaga tctggcccaa
    51721 cctgaacata tttatcaact ggcgggccct ggcctgcggg atgattttcc ggcactgcgg
    51781 acgctggaag cgtttcggca taatctaccc acccagttga cctcttttgt aggccgggtg
    51841 cgtgagctga aggaactgca aaaccgcctg tctgaacagc ggctggtaac cctgattggc
    51901 cccggcggca caggaaaaac ccggttagcc attcaggctg ccaccgagat gctggatcat
    51961 tacgccgatg gcacctggct ggtggaactg gcgccggtga ccgaaccaac tcaggtaatg
    52021 cgcgccacgg ccatgacctt tgggctgcgg gagtctgaag atcgcccgct gctacaagtg
    52081 ttgaatgatt atctacatgg caaaaatttg ctcttgattc tggataactg cgagcatgtg
    52141 attgatgcct gtgcatccct ggctgaagcg ctgctggagg tcaacccgca gctgcactta
    52201 ctggcctcat ctcgcgaggc gctggggctg accggtgaga atattttccg ggtgccctcg
    52261 ctggaagcac ccgacccgcg gcatttgcca ccctgggagc agttttcaca ctgtgaggcg
    52321 gtgcgcttat ttgctgaacg ggcagccgca gcccagcccg aatttgaatt gacgccggat
    52381 aatggcccgg cggtagcgca aatctgtcag cggctggatg gcattccgct ggcgattgag
    52441 ctggccgccg cgcgggtgaa ggtgttgagc gtggaacaaa tcgctgcccg gttggatgac
    52501 cgcttccggc tgttgacggg cggcagccgc accgctctgc cgcgccagca aacgctgcgt
    52561 gcgctgattg actggtctta tgatctgctc aacgaggccg agcgggtgct gttccggcgg
    52621 ttggcagttt tctcaggcgg ctggacgttg gaagcagcag agacgatctg ctcgggcagt
    52681 attaacgggc aggaactgac cgagtgggaa gtgctggacc tgatgagcgg gctggtaaat
    52741 aagtcgctgg tgctgacgga actacgcggc ggcgaaaccc gctaccgaat gttggaaacc
    52801 atccggcagt acacccgcga ccggctgctg gaatccggag agagtacggc ttgccgtaac
    52861 cggcatttgc aatattacct gaatatagcc gaacaattgg aaccggaact atggcgagat
    52921 caacagttgg cggcgttaaa acggctggat cgtgaaaatg ataatttgcg agccgccctc
    52981 gagtggtgcc tggcagagga aagcgggccc aatatcagcg caggactgcg gattgcctgc
    53041 acactgccgc cctactgggg gatacgcggt ctctggacag agtgtaatga gcgttttcaa
    53101 acacttttgg ggtaccccaa tgcagaggtc tctgaccggc tgcgactgaa ggcgctcaac
    53161 cgggcggggc tggctgcccg agagcaaagc aatagcaccc tagttcagct ttactttgga
    53221 gccgcactgc cgctggctga aaaactgggt gagcgcggtg aacaggccta cgcgctgttg
    53281 ggcctgcacc gaaccgacga atcaattgcc atttttagag aattgaatga cccggtgggg
    53341 ctgctgcgag ccctggctgc caggggcaca atcgcaaatg cagctggcga ttttgagacg
    53401 gcaaagcgcg catttgaaga aaacctggcg ctgtgtcatg ccattgggca ccgcattggc
    53461 gccgccggta cgctggtggc gttgagcttt ttggggcgca tggataacga cctggatgca
    53521 gccgagcgag ccgggcagga ggcgctggag attttccgca cagccgatga ccgcccgggg
    53581 attggtgggg caacctatat gctgggactg gtcaaaatgc agcgcggcga tttggatgcc
    53641 gcgcgggtgt atttcaacga aaatatggct attcaaaatg agctgggcgt ggtgccattc
    53701 attggtggag caaccctcat actatcagaa ctggagcggt tagcccaaaa ttatgaccgg
    53761 gcactggaac tgctggagcg atgtcagaag atttttgtgc gcgcccagga tccactaaat
    53821 ctggcactga cctacatcaa tcaggcggat attcttcagc ggcaggggcg catggatgaa
    53881 gcccgggagg cgctcaagat ttacttcgct tcaccgggga ttaattacgc cacctatgcg
    53941 ctggggttgt tgaatgtggc ctacctgggc tggcaggcag ggcaggcgga aaccggggtg
    54001 cgcttcctag gagcttttgc gggggttccg ggcattgaac agctgctgac cccggcagat
    54061 tatgagcaaa tggagactgt gaccaccggg ctgcgcgctt tattgatgcc agaagcctac
    54121 caagctgcgt atgccgctgg aatgcagctg gcaaaagaag acctgggcgc ggtgattgcc
    54181 gaggcggtgg attatttgag gtgataaaaa ggagggaaaa aattaaattg gaaaagccag
    54241 cttataaata cagtgatatc acccaaaaaa ttatcggtgc ggcgctgaaa gtccattcag
    54301 cgcttggcaa tggctatctg gaagtgattt accagcgcgc gatggtgatt gagatgcgta
    54361 agcaaaatct ttcctttgag cgcgaggtgg aggtgccgat tttttacgat ggagagcata
    54421 ttgggacgcg ccgcgttgat ttcattgtgg aaggcaaggt gattgttgaa atcaaggcga
    54481 caacgcagtt acaagatgtt catctggcac aggctataaa ttatctggaa gcttatgggt
    54541 ctgaaattgg tcttttgttg aattttgggg cgaagagttt gcagattaag cgattgatca
    54601 agagccgggg ggaaagctga accacggatt atgcggatta tggggctgcg gtaataagtg
    54661 aggtacgtgg gtgggggaat tcagaaccgc tgatgacgct gatttcactg attgggagga
    54721 tacggtaatc cgtaaagtct acggttgggg ggagttctga accactgatt acacggattt
    54781 cactgattac gctggtgtag taatctgcgt aatccgcgga atctgtggtc gggggagttt
    54841 agaaccgcgg attacgcaga tttcactggt tttgctgatg tagcaatctg cataatctgc
    54901 gaaatctgtg gtgggggagg tgaaccattg attaccctga ttttgcggat aggtaatcta
    54961 tgtaatcagc gcaatctgcg gttatgattt cggtgtatat acagccagac gggtatgcag
    55021 gggggatgtg ccccggcgcc agcggaccca caaccagatg agcagggatc caagggcgat
    55081 ggctaacagg ccaatcaggc ctgcttcggg gccaaaggcg ccgccggtcc agaggtcagg
    55141 gccgccctgc tgaatgccga tgagggtggc ggtgcggggt gtgccgctga cggggaagcc
    55201 aaagatggcg ccctggaaga agttccaggt aatgtggagg ccgatgggga tggccagttc
    55261 gccggtgaga acaaagccca ggccaagaaa gagtccagcg aggatgatgt tgaaggtgct
    55321 gattaggctg gcgttggggt tgcccatgtg cagcaggcca aataccgatg aggagatgag
    55381 ataggcaacc agcagggcgg cgcggggcat tttgccgcca aagttgaagc cttcggccag
    55441 gttgcgcagg tgatagccgc gcgaccaaag ctcttcgtag atgcccacgc aaataaactg
    55501 caccagaatc agactaaagg tggcccagaa gggcaatccg ggagtgtcca cctgccaata
    55561 gccggtgatg gtgacccagc cagccgccag ttcaacacca aagatgatcg ccatcagcac
    55621 ggcgccaagg atcaggccaa aggcaaaatc gagccaccag ccgcgggaga agtgcaggcc
    55681 aaagtccagc agggggcggc ggtctaaaaa cagacccgcc agccagagtg agagcagcat
    55741 ggccagggcc atgccaatga cggtcacaac gttcatcagg aaaaagtttg atggtatctg
    55801 actggacagg agggtcaaga caaccaccgg cacggcgcta attatcccgg cgaccagaaa
    55861 catgaacagg gcttgaatca ctaatcgcca gagcgagcga acgcgctttt caacgggatt
    55921 ccagaagggg ttagtcatgg gggattctcc aattatgctg tggtttcaaa atgcgcctgc
    55981 tccacctgct gccaggtttc gccgggggcg gtgcgcaggt agctgcccaa aagcggggcg
    56041 gcctgctgca ccagttcggc catgcggtgg gcaatgcggc ggatggagaa gtgggcgttg
    56101 ggggcagagc gcagggcgat gaagtgcatg agggagcgca ggttggtctg gagcagaacc
    56161 cgccggttga agccgttagg tacaatgtaa gatgccacgt tggggtcaaa ttcggccagg
    56221 gcagcccagg tttgatgggc ggcatcgagg gcgcggcgga catcaccggc aacgcctgcc
    56281 gcagcgaagc ggcgcggcag agcatagccc agcccagccg tgagcgcctg gggggtttgt
    56341 gtcatcatgc ggtggcgttt gagttcggca taggcgccct gatccaaaat caaatcaaag
    56401 gtaaaggtgg cgtattccag ttcacgcagg gggatgtcgt gcgcgcccag gtctgccagc
    56461 gcagcctgcg ccagttgaga acgcccggca ggcggtaaag cctgcactgc cgccaaagcc
    56521 tgggcataag actggctgcc aaaacgatac agcgccgcag ccagggcgcg gttttcagca
    56581 ctggcatcaa aatctaccag gcggcaccag gcagtttcat ccggggcaag gacatctaaa
    56641 gcgcggggca aagccgcaga ctgggccgat agccgcgccg cagttgattg caggtaaggg
    56701 acggcatcag catatttgac cagggtgggc acgctctggc gcgccgcaaa tttgatctcg
    56761 gcgccgatgg cctgcacctc agccagggga tgcgaaagca gcttgctgat ggcatgttcc
    56821 agggcgcggg cgttgattgt catgccgaca ttggcaagcg cagccgcggg cagtaaaaag
    56881 cggcaaacat ccacgctttg ggggcgcacg cgccgcagcc aggcctcttc tgattctgtc
    56941 gggctgcgcg ggtcttcacg ctgaatggct tccagcacgg cgggcaggca gcgggtatag
    57001 gtggcgaaca gatcgcggca ggtttcagcg aagcgttctc gcagcaaatg ccggtccagt
    57061 tcggggggaa taaaaaagtc ctccgggccc cattgctggt agcgagtgga cttttcggta
    57121 taagatgcca ggcggttaga ttcaagcgtc tcgactgcca ggcgggagat gttttcaacc
    57181 gcgatgtgca gcacggcatg ttcagccacc gaagcatgcc cgtagcccac cacccatttt
    57241 tcgtgaaaat cagcgctgcg ggcgtcggtc agttcagcgg caatctgacg gaaagactgc
    57301 ggtgaacggg aggtcttggc aaaggtcacc gcgatggttt cagggggcag gtcctgcggg
    57361 tcgagcaaat acacctgacg gtcacgcggc atgggcaaac acctcccggg cagattgggc
    57421 atcacgccgg atctgggcca ttaattcatc aatcgaatta aaacgctgct ccgtgcgcag
    57481 gcgggagaga aactcaagct gtaagacctg accgtatagg tcgcgatcga tatccagcaa
    57541 atgcgcctca acgcgcaggg ctggttcgcc gttaaaggtg gggcgcacgc caatattggt
    57601 gacggctggc aggcgctcac cgccggccca ggcccaggtg gtataaaccc cgttggcagg
    57661 caaaagccgg gagggagaaa tatctaaatt agcggtcgga aagcccagcc cgcgcccgcg
    57721 gccatctcca tgaacgacca ggccctccac gctgtgcggg cggcccagca agcgggcggc
    57781 agtgcccacc tcccctgcca gcagggcaga gcggatgcgg ctggatgaga caggttcacc
    57841 atccagggtg acggtaggga agagcgagac ttggtatcca agttctgcgc cgatctggcg
    57901 cagtttttca acattgcccg cacggccgcg tcccagggca aagtcgggac ccacgcgcag
    57961 gtggcgcagg cccagggcac gttgaacgcg ctgcatgaac tcatcggcag tcagagcggc
    58021 caggtcagca ttgaagggca gggtgatcac cacatccacg cccagctcac ccaacaggcg
    58081 cgcccggttc gccgggctgg tcaggcagta ggggccggtg ctgccgcgca gggctaccag
    58141 cgggtgcggt gtgaacgtaa tcacgaccgc gggagcatct gcggcggcag aatcctgcac
    58201 cagggtgcgg atcagggcct gatggccgcg gtgaacgcca tcaaacgtgc caatggtgac
    58261 ccaggcgccg gtcagatgca gcgcctccag gctgtcttcg tgacgcatta tcagctggct
    58321 acaaagactt ttttaggctg ccagccgcct ttttcggcgt cataaaccag cagagccacc
    58381 agctcgccat cggcagttac gccacgcacc atggcctcag cggcgccttc accggcgacg
    58441 atgtggccgt gccgcacttc gtcaatttgt ttttcatcca gcaccagggc aggccagtca
    58501 ctgagagcct cggcagcggg gatgatgtat tggccccagg tgccattatc aaaggcctca
    58561 cgcagtttgc gcaatggtat ggcatctcgc agggtgaaac ggccgctgcg agtgcggcgc
    58621 agccccacca gatgcgcacc gcagcccagc ttggtgccca gatcgtttgc cagagagcgg
    58681 acataggtgc ccgaggagca gtagacatca atcaccgcct ccgggggatc ccattccagc
    58741 agttcaaggc tgtagacgtt gatcttgcgg ggcgccaggt caacttcttc accctcgcgg
    58801 gccatatcgt aggcatggcg gcccttaatt ttgatggcgg aatagggcgg cggcacctgt
    58861 tcaatttcgc ccacaaaacc ggccagggct tcactgaact gttcttcggt gatgttatca
    58921 accggtgcag aggtcaagac ctcgccatcg gcatcgtagg tgtcggtaga aacacccagc
    58981 tcgatcactg cctgatagcg tttatcagaa gcagagacgt actcgctcag gcgaactgcc
    59041 ggccccagca aaatgaccag cacaccagag gcgcgcgggt caagcgtacc ggtatggccg
    59101 gcgcggcgca ggttggtgcc tttacgaatg acctggacca catcgtgaga cgtcagtcca
    59161 acgggcttgt ccacaaccag gacgccggaa atcgcatcat ttggatcttg gaacggtttc
    59221 cccatggctt tttacctctg tttcgtttat cctttttgta taagaattat aacatttttc
    59281 aagccgcggc aggcgcggct ttgagcagcg cttcggtggc ctggagtaca cgcgggcgaa
    59341 cttcatcgag cgtgccggga atatctgctc cggcagccgc actatgacca ccgccgccaa
    59401 agctgaaggc cagctgcgag acatcatagc cgggttggga gcgccagctg accttgacgg
    59461 tgccttcacg ctgttcgatg aacagcacca cgatatcaca ctcgtcaatg ctggagagga
    59521 tattggtcag gtcggcatca tcattgccct ggtaaccgct ttgtttgcga tcttcaagcg
    59581 ttaggctggt ccagcacagg cggcctttac ggtcgaggcg ggaaaggccc agcccccagt
    59641 agcgggtggc agaataagtg cgcacaatca gggcacggcg gtagagttcg ggcaggtcgg
    59701 ccccgtgatc aatcagcatg gcgccgagcc gcagggcatc ggcattcatg ttggaggtgc
    59761 ggaagccgat ggtatcggtc agcagcccgg tgagcagagc cgcggcggca tcgggcgtaa
    59821 tggacaatcc ccacaggggc agccgctccg ccagaatggc tgccgtggcg acagcctcgg
    59881 gcaccaccag gttgaggcgc ccataattca gattggtgac gtggtgatca atattcaggt
    59941 cgggggtgcg gccttccagg ggttcaccca ggcggcgcgg gtctgaggca tccagtgtga
    60001 ccagcacatc aaattcgcca gcgggctgct gcaaaatctg atcggcgccg ggcaggtgtt
    60061 tgaaggtgcg aatgacgcca tcggccagga ccatttgcac ctgcttgccc gcagcctgca
    60121 gcgccaggcc caggcccagg gttgagccca ccgcgtcgcc atcagggcga atgtgcgaga
    60181 gaattaatat ccgttcggcg ttggcgaaca gggtacgaat ttgatcatcc agggcggtgg
    60241 ccggttcaaa cattattcgg catcctcgtc ttcatccgcg gcgtcatcag ccgggggcgg
    60301 ggtgggcgtg tcggggcgga tttcagccag caggcgttca atccggtcgg cgcgctcggg
    60361 ggtggggtcc cagtgaaagc gcaggcgggg gaagacacgc aggtcaaccc gctcggcaag
    60421 catgcggcgc aggaaaccgc tggcgctgtt taagccagcc aggatttctt tggcgcgctc
    60481 ctgcccctca accgcagaga agtagacgtc ggcaaaggcc aattcacggt ccactttgac
    60541 atcggtgatg aaaacacccg tcagacgcgg atcgcgcacg ttgctcatca ccagcatttc
    60601 agataattcc tgccgaatcc ggtcggcaat ccgttgcagc cttaattgcg aaggcatggc
    60661 atctctcctt tgcagacttt agtccgcact ccaaacaaca gatggcgctc taaaatcaga
    60721 ttagaggctc ttctccagaa cataacactc aaccacgtcg ccgggttcga tgttggtaaa
    60781 gttgcgcagg gcaacgccac attcaaagcc cagacggacg acattcacat cttctttttc
    60841 atgcttcagg ctggacatgt cgccttcgta aagcgtttcg ccggcgcgga gcagacggac
    60901 cttgccattg cggcgcagtt cgccctcttt gaccttgcag ccagcaatgt tgccgacctt
    60961 gctgatcttg aagaccgcca ggacttcggc acggcccacc accacctctt tgatttccgg
    61021 cgccagcata cccttgagcg ctttctcaat atcttcggtc agccggtaga tgatattgta
    61081 gaggcggatg gagacgcctt cggtctcagc caggcgccgg gcagccaaat cggcctggac
    61141 gttgaagccg atcacaatgg cgtgggaagc cgttgccagc atgacatcat tttcactgat
    61201 attgccggtt tcggcatgca gcacgttgag cccaatggtg ctttccttgc ccaggttatt
    61261 cagctcgttg ataataggtt ccagcgagcc ctgcacatcg gctttgacaa tcagccgaag
    61321 ttcgcgctct tcgcctgact gcacgcggct gaagagttct tcaagggtgg ccttgggggc
    61381 tgcggcgcgc tgcgcctggg ccttggtgcg gcggtcttcc accacctggc gcgcttcacg
    61441 atcactgggc agcacctgga aaaggtcgcc ggcttcgggg acttcgctga ggcccatgat
    61501 ctgcacgggg gtggaggggc cagccttttg cagcttgcgg ccgcggaaat caaacatggc
    61561 ccggatgcga ccccaggcgg tgccagccag cacaatatca cccacagaca gggtgccgtt
    61621 ctgcaccagc agggtggcga ccacgccctt ggcgcggtcc actttggctt caatcacggt
    61681 gccgatggtg cggccattgg ggttggcctg aatatccatg ttctcggcta ccagcaggat
    61741 ggcttccagc aggtcttcga tgccgcggcg gagcttggca gagaccggca cgaccagggt
    61801 attgccttcc cattcgtcgg gcaccaggtc catgtcggcg agctggcgct tgacaaagtc
    61861 gggattggcg ccgtccaggt ccaccttgtt catggcgacg atgatcggca cacgggcggc
    61921 gcgggcgtgg gcaatggcct cacgggtttg gggcattacg ccgtcattgg cagccaccac
    61981 cagaacaacc acatcggcgc cctgggcacc gcgggaccgc atggcggtaa aagccgcgtg
    62041 accgggggta tcgaggaagg tgatggtgtg gcctttatgt tccacctgat aggcgccgat
    62101 atgctgggta atgccgcccg cctcgccgcc agccacgttg gtgtggcgga tggcatcgag
    62161 gagggtggtt ttgccgtgat cgacatgccc cagaatggtg accaccgggg cgcggttggc
    62221 aaggttgcgc tggtcttcgt tggcaatgag ctgccgccag agggggattt cgcccgtttc
    62281 tttttcttca acaatatcaa tggcttcagg atgggcctca aagcccattt ctgaagccac
    62341 aactgcggcg gtgtcaaaat cgacctgttg atttatgttt gccatcacac cgacatccat
    62401 caggattttc atcaccttga tggggctggt ctccagcttt gcagccagat cgcgcacgat
    62461 gatgacggct ggcagttcga tctgcttctg cccttgttca ctcataaaac acctcctcgg
    62521 cacttccggt aaagatgccc cctccgccag accgctccct ggcgatgaaa cccaaaaggg
    62581 catcagcttt ccggcggcgc cgggcagggt gcgggcaaat tagcggttca tctttgttaa
    62641 gcgttcaggc tgccggttca cgcggacacc acaggggctg gcggcgtttc ttccggcaaa
    62701 gtctggacaa aggcttccaa ttgggtgcga tccgagtcgc tcagttccac gcgcagggcg
    62761 tttgccaggg cgcctttgag ggcgcgctgc caacagtctc ggctgttgtg cacataggct
    62821 ccccgcccgt ttgccttgcc cgaagggtcc agttggacac cctcgggcgt gcgcaccacc
    62881 cgtgagaggg tgcgcttggg cattaccagc cgacaaccta cgcaggtccg ctggggggta
    62941 tgtttgactc ggggtttgac tttcttggtc acctgtcctg actctaaatc acaatcttaa
    63001 agttcccaat cgtcatcgct ttcctggtga gggctgcgat agacggcggc gccggtgtcg
    63061 gggtcgtatt cgaccacggg gcggcgtttc ttcttgccgg gcttcttctt gccaggtttg
    63121 cccttgctgt cttcctcttc ctcctcatcc acggcaatgg ggtgcagggt ttcggggcgc
    63181 agggagaaga tctcttcaag ggagggctcg gcttcagttt cagcttcggc agcgggctca
    63241 acaacggctt cgggctgggc cacaggttcg gcttcgggtt cgggttgaac cggggccgct
    63301 tccgcttcca cttccacaac aaccgcttcg ggttgagcca caggctccac ttcaacttca
    63361 acggcagcgg gcgcagcctc ggcttcggca acagcagccg catcagcctg ggctttttcg
    63421 gccagagccg tcatcaattt attgatctca tccatcgctt tgcggccaat gccgttgagg
    63481 cgcaggatgt catcgggcat tttcttcatc tgcagggcca ggtccgatac ggtggcatag
    63541 ccggcctctt gcaggatgta gcaaacgtgt tccggcaggc cgctgtcgag aatattggtc
    63601 tcgtaggcgc tggcggggat atcggggtga gactggcgag cttcaaccgt gtcggcgggc
    63661 ttctcggcgg caaagcgaac cgcgcggcgc tgcacccgat cgacaaactg gcccatctga
    63721 tcatattctt ccggcgagag cgagcggcct tcggccttgc gaaccagcat atcgctgatt
    63781 ttctcaacca gggcagcatc cacaatggcg gccagggagg catcttctgt gatgtttttc
    63841 agggcttcgc tggcggcttc gggcaggctc ttgatgtcaa tgcgccagcc ggtcagcttg
    63901 gccgccaggc gggcgttctg accatcgcgg ccaatggcca ggctgagctg gtcttcgggc
    63961 acaaccacgg tagcggtctt gccattgaca gactggtcat tcaaatagac gccagtgacg
    64021 cgcgccgggc tgatggcctt ggcgataaag atggcggggt cggcgttcca ttcgatcacg
    64081 tcaatctttt cgtcgtgcag ttcgcgcaca atggcctgga tgcgaacacc gcggatgccc
    64141 acgcaggcgc ccaccgggtc gatgccgctc tgggtagctg agacagccac cttggcgcgc
    64201 tgaccgggtt cacgggcgat ggagcggatc tccaccacgc cgtggaaaat ttcgggcact
    64261 tcggtttcaa gcaggcggcg caggaagttg cggtgcgtgc gagacagaat gatctgcggg
    64321 ccgcgcgggc tgtctttgac ctcagcaagc agggcacgca cacgatcgtg tatgcggaag
    64381 cgctcgccag ggatcaattc tttacgcggc atggtgcctt cggccttcat ttcgaggcca
    64441 atggtcaggc cggtggcatt gacagcctgc acaacgccgc tgatgatttc gccaatctgt
    64501 ttttcgtaat ggtcaatttg agcctggcgt tcggcatcgc ggatgcgctg ctgaatgacc
    64561 tgccgggctg tctgggccgc cacgcggcca aagtctgccg gggtggagtc cacaatgacc
    64621 atatcgccca gttgggcatc cgggttgact ttgcgagcct cagcgagggg cacctcggta
    64681 tgcggatcga caacttcttc cacgacttct ttttccgccc ggaccgatac cttgccggta
    64741 tcaacatcca cggtggcttc tacatgctgg gcagccgaag cattgacggc gcggcgataa
    64801 gccgacacca tcgccatttc cagcgccttc aggatcactt ccttggggag ctgtttctct
    64861 tccagcactt cattaaaggc cagggtaaat tcgttcttca tccttttcgt tctccaacca
    64921 tcacccatat atgcagaaaa gtggggggcg cccacttttc agtgcttgcg ttacgttgtt
    64981 ccaacgggat tattttagca cagagccaaa tgtttcgcaa gggattgggc gcgatttttt
    65041 cgcggctggc gcagggcttg actttttttt caagtggcgc aatgatagtg taatctttca
    65101 gtatcggaga aagcaggaaa aacgatgcgg ctttatgaga ttttgatcct ggcgggaaat
    65161 gggctgctgg cagtgctgat gctgctgcgg gtgcggcggg ccttgccgct ggcggcagga
    65221 gcactgacgc tgctggcaac actactgcac gtgctgctgg aaggcacacg ctggcagatg
    65281 attccgcttt acgggttgac ggcgcttttg attggactga ccctctaccg ggcacggcaa
    65341 aaccctgccc cacgccggag atggcgctgg gtatggattg ttgcggcggc agcctgtggg
    65401 attctgcctg cgctgatgcc cgtgcccaaa ctgccgcagc ccaccgggcc ttatgacgtg
    65461 ggtacaacaa ctttttactg ggtcaatccg gatgcggttg atccgtacac cggggaggtg
    65521 cggcggctga tggtgcaagc ctggtacccc accgataagc cctccaccgc cgaacctgcg
    65581 ccttacttcc ctgacctgaa ggtgatggcg ccagccatcg cgcgcaattt cggtttgccc
    65641 gatttcatgc ttaatcacct ggagttgagc cagacgcata ccacgctgga accgcccttc
    65701 gccagcgact tgacccagac gccggtgctg attttctcac acggctggac gggcatgcgc
    65761 tatcaaaata cctttcaggt cgaggaactg gtcagccatg ggtatgttgt gattgcggct
    65821 gatcatccgg aaggggcggt catcaccatt tatcccgatg gggacgtgat atttgccaat
    65881 caggccgccc tgccgcgcag cgcaccggcg gcggagttta atgcggcagc ccggattgtg
    65941 gggcaaatgt gggtcagtga tttgaggttt atgcgcgctc agatggatga cctgcaaagc
    66001 ggcgccattg ccagcccgct tgccgggcac ctggacctgg cgcgggtggg cgcctttggg
    66061 cactccaccg gcggcggggc ggtggcagag ttctgttaca acaatgagga ttgccaggcg
    66121 gtattgacca tggatgcctg gatggtgccc tatgaccggg tggtgccggc gcatgggctg
    66181 acgcagcccg cgctgttcat gcggagcgag aagtggtcgc tgccgggaaa ccctccgctg
    66241 gctgaggcgc tctatgaaaa cagtcacagc ccggcttacc tggtggagat tgccgatacc
    66301 cggcactttg attttaccga catttcgcag cttaccccgc tgctgacgct gatgaagttg
    66361 aaaggtacgc tggattctga ccgggcgatg acgatcatca atacctacac actggccttt
    66421 tttgatgagg ccctgcgggg ggatgcgtct ggagtgccag cgctggaagg gctttacccc
    66481 gaagcgccgc tggaaatcaa caccggcgcg ccgtaaggtt aatcatcacg ctggcgcacc
    66541 tcagcccggt actggcacca ggcgcagttc ggggcagctt cgggcggttc gggctgttcc
    66601 aaaacggcaa gcaccttgcc gataaattca agaaaaccgg cttcgttcag ggggatttcc
    66661 tgccaggtga ccgaacccag ataggcgatc tgatcggata tctgcgtcat ctgaatgggc
    66721 tcgaccacca gcagccccag gcggctgatg gggctgagcg agagcttgcc gccggctgcg
    66781 tgttccaggg cataggcgta ggcgtgaagc tgccgcccat aaaaagccag atgcgatggg
    66841 cgcgcctgcg aggtcttgaa atctaccacg ccgtaagagc cgtcttcaaa cgagaccacc
    66901 gtatcgaact tgcccagcag atagcaggaa tcgacatgcc cgggcagggt gatgggctga
    66961 gactgcaccc agcgctcgcc gaattgaacc ttgcccggcg gcaaaccggg gtaaatggca
    67021 gtggttgagc ggccctcaaa ataagccttc atcaggctgt caattttgga gaaaatggcc
    67081 ggaaaggccg aagccgggcg gccaaacttg cgcaccacct tcaggtagaa gcagcgtggg
    67141 cattcgtccc acaaaaaggt caggtcagag gggctgagtt tatgcaaagt catggggcag
    67201 atcctggggg atgggatgtc ttcatcatat aacagagcgc ccaaaaacac aaatcaaaag
    67261 cggcccctcc gaagtggaag ggccgctaga aagcggatgg gattatggcc gggccggggc
    67321 atcatccagc aggcggcgct cgccttgcag ggcgcgaatg ccggcgacat cctgggtgac
    67381 ctcgagggcg ccgtggtatt tgccagcggc atcacgcagg gcgaagtagc gaatatgcac
    67441 aaacttgccg cccatctgaa tccagaactc ggccacgctg cgcaggccag cccggaaatc
    67501 atccaggatt tgctggacgc ggtgcacgct ctgcggcggg tggcagtttt gcacggtgcg
    67561 cccaatgatg gccggggtgc ggtcaaaaat gcgttcacgg gtctgggaga agaaccgcac
    67621 ctcatcgttc tcgtccacat aggtaatatc caccggcaag ttgcagagca tgaggttgat
    67681 ttgctccaga gagagtgcac cggtgctgag cgccagcagg ccttctggca ggctgccctg
    67741 cggctgggcg ggcggttgcg gatcggcggc atgcaggtcc tgcgggcgcg gcatccattc
    67801 attgccggga gtgaccaggc agtagccaat ttcggtttcc tggtcgcgga tggcgaccca
    67861 gtcggcttct ttgaggcgct ccagggcggc ggggaagagg attttctctt ctttataaac
    67921 catctcacgg atcatcatgg caacggcatt gaagagtgcg ctgacctttt tcagttcggg
    67981 ggtggcctct gccagggcgc tcttgagctg gcgcagcgcg ccgcgaatat catcatgaat
    68041 gccccacatc acggttgaag ggccgcgaaa accaacctgt tcgaggtagg ggaaaagcat
    68101 attttctttg cgcagatagt ggcggtcgag ctgcatcagg ttttccaggc tggcggccag
    68161 ggggcgggcg gtggagtcgc cttcggcatc ccagcgggcc aggtcagcgg tcatggcgtc
    68221 gagcaggcgc agcaccattt gattttcggc ccggaaggtg tgcaccgggt gtccggggat
    68281 agcttcggga gggcgctggt tatcaaggtt gtcgcggaaa acggcgacat gcacatcaca
    68341 caggcgttga atttcttcgg cgggcaggcc ctcattcatc agcatctgtt ccacttcggc
    68401 aatttcggct gaactgattt cagccgccag ggcgccaaat tcggccttga cttcttcgag
    68461 gcttttgcct tcatgcaaac cgcgcagcac attgaggatg atttgcttgc ggcgggttac
    68521 gttattaata tgttcactca ttgcgggctc cttcaatcaa ttgatgagat taatgatcaa
    68581 gtatgcttag attatgccag attcagacgg cagcgtcttt gcgctggcgc aaacaacagg
    68641 gcgtgataca atttaatggt gccaaattta cccctcaaac ccgaattact ccgccagacc
    68701 gagctgtttg ccgggttgac ggataccgac ctgcagacaa ttcaacaggc ggctactgtg
    68761 cgccaggtgg agagggacgg ttttttcttc tatcagggcg acccggcaga gcaggtgctg
    68821 gcgctgacgg aagggcgggt gcggctggcg caggtgacac ccgatgggca gcaggtgatc
    68881 ctcaacctga tttcagccgg agcgctgttt ggcattattg cagcaatcaa ggccacagac
    68941 tatcccgttt cagcccaggc aatggaagac agcacagcgc tggcctggtc tcgagaggcg
    69001 atgatggggc tggtggggcg gttcccgctg ctgggaatga atatcatgcg gctgatggct
    69061 gagcatgtgc aagaaacgca gaaccggctg cgagaactga ccaccgagcg ggtggaacgc
    69121 cgactggcac ggacactgct gcggctggcg gctcagaccg gagtgaagac ccccgaaggg
    69181 gttgagattg gcctggcctt gacccggcaa gacctggccg agatgattgg tgccacgctg
    69241 tacaccgtca gtcggatttt aagccagtgg gaagcacagg ggctggtgag atcgggacgg
    69301 gagcgggtga tgatccggtt tccgcatggg ctggtcagca ttgccgaaga tctaccgcag
    69361 cgggaataaa ttgcccgggc ttatttgcgc tggcgcaaag aacatcaagc aaaatcgggg
    69421 tatgattaca acatgatgag ccaacccctt gatccccagt gttcagtagc caccctgctg
    69481 cgtcagtggc cagcagccgt gcccgttttc ttacagcggc gcatggcctg cgtgggctgc
    69541 agcatggctg cgtttgaaac cctggaagac gcggcccgtg tgtacaaaat tcaacctgcc
    69601 cagttcctgg aagaactgca gcaggccatt caaaattcag ctttggagga aaaaccatga
    69661 ccacccccta tcaatatttt gccagcctgc ccgccaccgt ggacagcatt ccagcggaga
    69721 gcattgtcag ccgcgagctg cataaagacg accgtgtgaa agcgattgta tttgcctttg
    69781 cccccggaca ggagctttcg gagcatacag cctcggttcc ggcgatgatt cagattctgg
    69841 aaggcgaatg caccctgacg ctgggcaaag attcgtttga ggccaagccc ggcgcctggg
    69901 catttatgga gcccaacctg ccgcacagca ttctggcgca tacgccggtt aaaatgctgc
    69961 tgattatgct ggcaaagtaa ttccataact gatgtacaat gaagggacgc aaacgactgc
    70021 gtcccttttt gtattgagga aacccaactc atctcttgag gtgaccgata tgatccctga
    70081 aaccgaaatt tttatccgtt tactggttgc tgcggcgctg ggcgcgctgg tggggctgga
    70141 acgcgagcgg caaaatcagc ccgcggggct gcgcactcac atcattctgg tcattggctc
    70201 ggcgctggcg atgacgcttt cgattaacct ggcgacacag taccgcgaga ttgcgccaaa
    70261 cggcgacccg gcccggctgg cggcgcaggt gctttcgggc attggctttc tgggagcggg
    70321 ggctattctg cgttacggca ccaacgtgaa aggcctgacc acggctacct cgttgtggac
    70381 catggcggtg gttgggttgg tgatcggtgc agggcattac tggattgccg tgattaccac
    70441 cattttgctg ctggcggtac tgacaatcct caacctgctg gaaacgcact ggctgtcggc
    70501 ttatctggtg cgcgtgatta ccatctcgtg cgatgtgcgc ccgcggatta aggaagacat
    70561 cactgcggcg ctgaacaaga ttaccaaacg ggtacggctg gcgagcgtgg aacgcaacct
    70621 gcggcatgcc cggcagcagt tgaagtacac catccgcgtt tcagaacgtg atgatctgac
    70681 cgacctggaa tcggcgctca gcgcgattga gggtgtgcga tcttatatga ttgaataacc
    70741 tagctgccat tgtgagctaa cagccattcc cggtggagga tggcatacca ggcttcatcc
    70801 acataggcgc cgttgag
//
LOCUS       DAOPVS010000003        62734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_418_length_62734_cov_55.388280, whole genome shotgun sequence.
ACCESSION   DAOPVS010000003 DAOPVS010000000
VERSION     DAOPVS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 62734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62734
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_418_length_62734_cov_55.388280"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PKL11_00660"
     CDS             complement(<1..1054)
                     /locus_tag="PKL11_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ12134.1"
                     /translation="MNKTDITGREPMFSLNRPKEAGGWRLSVQNLGIVLNTAAPEAVS
                     IEAAEAALNHKDYYVRYNAARLLMRRSDQPARQAMQRTLQSGAVPARASAARYLYGFS
                     WYTARPMLKKALQDQDARVREAAVYALCDCRQPAAYQMLIEALAEENDLVRAAAAWGL
                     RTHSDPAAVLVLEQVLKAEDEDVRVEALETLGLMEIPQGIALVLHSLDDPVLAVRYAA
                     GLSWLELCGTACLKDYATRIEQARGENRQPLLRALFHASNYLGIELSAPDVLATLLPA
                     LLAALHDAHPAVRLGATWPLAWMQHPQADEALRSAFAVEMDADVQAEMLYIIHSLSPK
                     LGAELLLTAESSPAEVV"
     gene            complement(1065..2636)
                     /locus_tag="PKL11_00665"
     CDS             complement(1065..2636)
                     /locus_tag="PKL11_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNZ12135.1"
                     /translation="MPHQLAIDFGTTNTVLARWNADLNYAETVMLPGLSQPAESSQPA
                     LVPSLVYVDSEEVVVGHAVRQRGLDRQRTQRLFRNFKRGIVAPQPESKRVIDGNRWTD
                     WDAGRAFLSRVLGALPFPISELETLVIATPVVAFEQYITWLDESVGNLSREQIRVIDE
                     STAAALGYAVTEPGAMVLVVDFGGGTLDLSVVQLPESGSKVGGVLRTLLGLKGTSTCT
                     ARVLAKSGQMLGGSDVDHWLAEEALRQIGINADSLSDDETPLLTACEALKIQLSSQER
                     AELAFNIGEQTHHLQFTRAELEDVLEAHGFYAAMRRVVEKTMYLARQRGIYREDIRFV
                     LMIGGTSLMPSVQQLMHRYFTEGIVRCDKPFTAVAEGALQLARGASLEDYLTHSFGLR
                     HLENGTPSFSEIIPMGTPCPTPKPFELTLCAAHPGQQEIEFVIGEIDSETIEKVDVRY
                     EGGVPVFVTGDDELAVQTLEGNVCVPLTPPGKPGEDRLKASFSIEADGLLKLSVTDLQ
                     TGKMLLENHPLTRLR"
     gene            2705..3367
                     /gene="pxpB"
                     /locus_tag="PKL11_00670"
     CDS             2705..3367
                     /gene="pxpB"
                     /locus_tag="PKL11_00670"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNZ12136.1"
                     /translation="MPAVSNLTVHPYGDSAVIVYLGSQLDRNVNQQVHALASRVMQAQ
                     LPGICEVVPGYVSLVIHFDPLQTSLPAVMDGVNDLHASSGQQPLPPARRVEIPVRYGG
                     EGGPDLDFVARHAGLTTAEVIRRHAAAEYTVYMMGFLPGFPYLGGLDVSIAAPRLETP
                     RRRVPVGSVGIAGEQTGIYPLASPGGWRLIGRTEVALFDLTAHPPCLLAPGDQVQFIP
                     IR"
     gene            3586..4821
                     /locus_tag="PKL11_00675"
     CDS             3586..4821
                     /locus_tag="PKL11_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ12137.1"
                     /translation="MRTFNRISIPILAALILLALWGCGPQTPLPPSHSVTPTATPLIA
                     YSLTPAPELPSTTPTPAGALPIWAPFAAPRQTPVTPIPAPLTGLSLPDEVRVLAVLGM
                     DQPAPYPGRTDAILLIIYHPRLAKASLLSVPPDLLGYVPGQTMQRLNTAYAVGDFRTI
                     SDALAYNLGLRPDDWLVIYEDALIWFVDDIGGLEITLLEPLPPHACGGIPAGPQVLNG
                     EQTLCYLRYRDGSDELARNFRQQEVARILFMGLLESGSLVNLPDLYSDYRPFIETSLS
                     LEDLLLAVPLAVQLGDENRIGYFQIQPADLTPWEISGPIPAPVVLSIPDAVRQRIQQA
                     INFVLTPEPFTNTVLTLEAALTVAPTPTQTPTLTLTPTPTVTYTPTITLTPTETLTPS
                     ITPTPTQTFTPSPTATPTP"
     gene            complement(4825..5775)
                     /locus_tag="PKL11_00680"
     CDS             complement(4825..5775)
                     /locus_tag="PKL11_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNZ12138.1"
                     /translation="MKSYVKLGILILAMVLAGCTATPTPTAQPTRAGAFTPLPSATPL
                     PSATPTSPYTATPLPTPTPTPRTHVVKANEDMFGIALRYGVPVEVIKTLNPTVDPRIM
                     SIGTVLLLPEVTPPPPGEATPTPTAAPLQLGEPACLPDAGGGWWCFVTASSESRLENV
                     AVLMQFTDPQSGQEAAETATLPLNVLPAGQALPLAAHFSNALPAGKPALQLVSALPLA
                     ADDPRYMPVQIVNPTTTIDINGKWAAVSGQAALSEGAVEARWCRVLAVAYDEAGAVIG
                     LRWWDSPAPLSTGDEAEFAFTVYALDGQIAQVALFVEAGQ"
     gene            5908..9114
                     /locus_tag="PKL11_00685"
     CDS             5908..9114
                     /locus_tag="PKL11_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ12139.1"
                     /translation="MEAFLGLLVGALGYGLVEIAKKTADLVIDPALEPAKNQIKAWLQ
                     KNFTQQQRDDRLKTTILQALTQLSLPKDEDPLRAWAQQTALDRLTAPANAALRRQFAL
                     AVLAHTNPHAEPPPDLLLALRWPLPRRAELTSLLTALRQKLADNPDWQPLLHYFTEAA
                     QLNLLTGLLSAVQKLLDAVIQTPSGPALLTVSGQPVMPEADFWQIQAGYCDKVKKCWS
                     EHLISGLAPEAGRPVQMPLAEMYFEPALLPARDPRERIEEEQKMLTRDALERLNHEMA
                     RGRDRLVEAFGQHPRLVLLGKPGSGKTITLHYTALMLAQGESGAARLGLPASALPILI
                     RLADYAINLTTNPGQTLHQALTEEFLKLCPYVEHASLFLEKALQQGRCLILLDGLDEV
                     GDFGQKLIDGQTLHQVVLKRIREFEQLYCRSESCNRILITSRLEGYAPGSLPGFAEAE
                     LSPLSFPDEVQTFLVYWFTAYRQRTLSLTWDQARYQAAQSVQDDLMPKIAASPGVTLL
                     ATNPLLLTILALLFAMGKRLPNQRVELYKMVVMTMIENWRHAQTDHASRLFDHIQPRD
                     ILRVLAELSFWLHSQLPGGTMMADQWQAQTHSQLLKNDLCLPKEAAALTEDFLDYARR
                     ENGLLTERSAGRFGFFHLTLEEYLAAWKIAHKPQEERQRILVERWPDPYWEEVLLLTA
                     GELQDGEEDALKNFLLSILALESTDPQLAGRPALLAGRALADIQNRKRSLNELVIPAL
                     QAVAQDTDPISRLPHTPGRVPVATRAAAAEVCDELGWLPPDLDDFIPLPAANLRVGKY
                     PVTNSQYARFLFADDFAHPPHWTDFPQFDEQSRPMGPSTDAAGLRWLETARQDPDLSP
                     DGKRVLPRYWTDPRLGASRPGAPVVGLTWYEANAYCQWLAAHWANPALHGGQAPGWGI
                     ASARLLTEADYRQAASAAQYPALDRFPWDAETPTTDQAEILRRANLGPADLNRTTPVW
                     MYPAGQSLLHIFDLAGNCWEWQANYYDKNHNLPALRGAAFLDGVGLARLSGRLWGSPL
                     SREGARRLSGLPGLPITLGAGTLRSGTLARPQFSRQPA"
     gene            9134..9478
                     /locus_tag="PKL11_00690"
     CDS             9134..9478
                     /locus_tag="PKL11_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNZ12140.1"
                     /translation="MPTPEMPIFTRTFDFLTWLLPTTQSFPRAHRQSFTLRLLGAAFE
                     LSDQLEVANRRSGAERRHALALADEALDRVRVYLRLAVRWHWLTPGQYRHVAVMTVEI
                     GKLLGGWIKTVH"
     gene            9862..10929
                     /locus_tag="PKL11_00695"
     CDS             9862..10929
                     /locus_tag="PKL11_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNZ12141.1"
                     /translation="MYAQICSWDNLWLAFQKASRGKRSRPAAAAFEHRLEDNLLDLQA
                     DLLNFTYTPGPYDSFYIHEPKRRLISAAPFRDRVVHHVLCNLIEPIFERSFIFDSYAN
                     RVGKGTHRALNRAQQYARRYPYVLQCDIRQYFPCIDHAILQNILAQKLKDPAVLWLCS
                     RIIASGASVLSETPEQVYYPQDDLFAALRPRGLPIGNLTSQFWANVYLNGFDHFVKQT
                     LRCPAYIRYVDDFLLFAHTKAALWQYRSALQQRLAQLRLSIHAGAHPFPVTEGLPFLG
                     FIIHPAWRRLKRRKGLYFCRQYRRKQQQYQQGLIALSTLTASAQGWINHLRYANTTGL
                     RQAVFQTPIPPPNRQTLPPTA"
     gene            complement(10942..11862)
                     /locus_tag="PKL11_00700"
     CDS             complement(10942..11862)
                     /locus_tag="PKL11_00700"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNZ12142.1"
                     /translation="MFELVFLGTSASAPSAKRSLPALVVKHDEHRFLVDCGEGTQRQI
                     LQSGIGFKRLNQILITHGHLDHILGLAGLLSTYLRWEAIDELTIYGGRAALERIYDLL
                     YGVVLRGAQAPMPIQMQAVERGTFWSVDGFSISAFPVQHRGPDCLGFVFEEPGRRPFL
                     PEKAEALGIPAGPWRRDLAAGQAITLPDGRTIEPETVLGDFKPGTRLVVVGDTGETDS
                     LVEICRGADGLVIEATYLDEEADMARQFNHLTARRAAELALKAGVGALYLTHVSRRYR
                     EKDILAEAQSVFPTAVVARDFDSFTVKAAG"
     gene            11980..12468
                     /locus_tag="PKL11_00705"
     CDS             11980..12468
                     /locus_tag="PKL11_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HNZ12143.1"
                     /translation="MELIPVQIQKPEEINFILGQSHFIKTVEDLHEALVNAVPGIQFG
                     LAFCEASGERLVRWSGTNAEMIELARQNALAIGAGHSFIIFLAPGFYPINVLKAVQNA
                     PEVCRIFCATANPTTVIVAQLGAGRGILGVVDGDQPIGVEDEAGIAWRTQFLRRIGYK
                     LG"
     gene            12544..13494
                     /locus_tag="PKL11_00710"
     CDS             12544..13494
                     /locus_tag="PKL11_00710"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ12144.1"
                     /translation="MKRIIPLLLVFSLLLAGCSILNFGQAQPTPSDADMATQVALVLT
                     NQPSPTSPADVPTQQIEALPTVALPTPTINIPATATVQPKPTDGGGAPTPTALSISPT
                     PLVQPTDTPTPLPTPTFPTNDPRQTLGNPTFTDKMDNSDYWPTGEDTYTAVTFRDGKM
                     LLTALTETDGWRLITYGPTANFYVEITARFEACSGTDHFGFYARVPEKNPATRGYLIG
                     INCLGQYRFAEWDGTTDPGTWTTHVYWTTNAAILAGANQTNRIGLLAENKHLTLYVNG
                     VKVGEMTDESYQSGYLGLFVGSDETQNLTVQVDEFNFWAR"
     gene            13615..14409
                     /locus_tag="PKL11_00715"
     CDS             13615..14409
                     /locus_tag="PKL11_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNZ12145.1"
                     /translation="MTDLGPYRPLIDALPVDLPALVDCLQHLVVHIFWAKRYGLQLTE
                     DREKEVQLRPAQAKLARLLELDARPLNEPRPLDRRLVCNCRDITTLLCAIRRAQGYPA
                     RARCGFATYFIPNHYEDHWVAEVWDASAARWVLADAQLDTLQREVLGITFDPLNLPPG
                     QFINGGAAWQLCQTGQADPDNFGIFEWHGWDFVRGNVLRDCLALTRVEVLPWDFWGLL
                     NTPWAESSPADRKLVDDLAAASAAGDASAVQAYLAVDPRLPFPSAV"
     gene            14430..15371
                     /locus_tag="PKL11_00720"
     CDS             14430..15371
                     /locus_tag="PKL11_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12146.1"
                     /translation="MTAVAIRTQNLTRTFETVRAVDGISFEVPSGIVFGFLGPNGSGK
                     TTTIRLLLGLLEPTSGGAEVLGFDTATHADDIRQRCGALLEHTGLYERMTAEDNLEFY
                     GRVWHLNAAERQQRIHDLLDAVGLWDRRKEPVGNWSRGMKQKLAVARAMLHRPQIIFL
                     DEPTAGLDPIAAVALRDELAALVQQQGVTVFLTTHNLAEAEKLCQQVAVIRQGQLMMI
                     GSPDELRLRQGGSRLEIVGRGFTEAALSILRQQPQVKEVTRQDQHITIALKDDADTAP
                     LVQLLVSQGVQIDEVRRGKASLEEVFLTLMEEDKHVE"
     gene            15361..16098
                     /locus_tag="PKL11_00725"
     CDS             15361..16098
                     /locus_tag="PKL11_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12147.1"
                     /translation="MLNDIFTLIWKEWKEVFLQRSSGRMGLVSLLIFVGMFGIFLPLQ
                     SGVEWLVSPMSMALAMWMPIFLVLGLVTDAFAGERERHTLETLLASRLSDTAILFGKI
                     GAGVAYGFTFALVTLIVGAVTINIAFPAGGLKFYTPALFFGGLAFSLLICLLMASLGV
                     LVSLRSPTARVAYQKLSIVMLVIVLLPTLLLPLLPEDLQMSVQMALQNINWGAIMLVA
                     AVVLIAAVAVLIGLAKARFQRAKLILD"
     gene            16207..16704
                     /locus_tag="PKL11_00730"
     CDS             16207..16704
                     /locus_tag="PKL11_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNZ12148.1"
                     /translation="MTKTLDNLQAAFAGESQANRRYLAFAKKADEEGFHQIARLFRAA
                     AHAETIHAHAHLRAMDGVKSTAENLQEAIGGESYEVTTMYPPMIEEAKAEGATKAAKS
                     FSMAWEVEKVHEALYRHALESIVQDEEEYDYYVCPICGYTHARTAPNKCPVCGAPGSK
                     FEVIK"
     gene            16899..17255
                     /locus_tag="PKL11_00735"
     CDS             16899..17255
                     /locus_tag="PKL11_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNZ12149.1"
                     /translation="MLQDYYPLIIIFTLALALGFLVVALGTIFGPRRPTKRKGAPYES
                     GMVPYGQGKRRMSVRYYLIAVLFILFDVEIVFFLPWAVILRELGIPGLIEMILFLLVL
                     MVGFIYAWKKGALEWD"
     gene            17246..17728
                     /locus_tag="PKL11_00740"
     CDS             17246..17728
                     /locus_tag="PKL11_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNZ12150.1"
                     /translation="MGLEQKTGNLGVVTVKLEDAINWSRTNSMWPMLFGLACCAIEMM
                     AAQAANYDMSRFGMELMRASPRQSDLMIVAGRVSRKMAPVLRQLYDQMPDPKWVIAMG
                     DCASCGGVFNNYAIVQGVDEVVPVDIYVAGCPPRPEGLINGVMALHEKVKREKLKDWA
                     "
     gene            17730..18242
                     /locus_tag="PKL11_00745"
     CDS             17730..18242
                     /locus_tag="PKL11_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNZ12151.1"
                     /translation="MDAHNQPIAALFVDRFGAQAAEFRGELTLTLPADQAPAAALAAR
                     DELGYNLLLTATAVDYWPQQSPRFHVIYQLRSLKTNYVLRLCIPLNGNAPVMPTLEGI
                     YPNANWKEREIWDMFGIRFEGHSDLRRIILPADWEGHPLRKDYPLGYEEPQFTFNFDE
                     IRLHKPRGEE"
     gene            18247..19476
                     /gene="nuoD"
                     /locus_tag="PKL11_00750"
     CDS             18247..19476
                     /gene="nuoD"
                     /locus_tag="PKL11_00750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNZ12152.1"
                     /translation="MSDIQEVNLGELTHLVSPRALTGETMLLNMGPQHPATHGVLRLL
                     LELDGEIIITCIPDIGFLHTGVEKNMEAKTYQKAEVMSDRLDYLNPIGNNLVYCLAVE
                     KLAELDVPPRAQALRVILAELQRISSHLLWLGTSALDLAAMSVFLYGMREREIILDIF
                     ELVSGARMMTTYVRPGGLWRDVPVEFEKAVRDFLALMPRRLAEYDALLTKNTIFLERT
                     RNIGVISAEDAVRYGLSGPVLRGCGVAHDLRKDAPYSGYEQYDFNIPTQTAGDIYARY
                     LVRVQEMHESLKIVRQALDNLPYGPVRSNNRKFVPPPRSELGTSMEAVIHHFKLWTEG
                     FSVPQGAVYVATESPRGELGVYLESDGGPKPYRIHFNTPTFANLQALPLMVRKAFVAD
                     VVGVIGSIDIVLGDADR"
     gene            19473..19988
                     /locus_tag="PKL11_00755"
     CDS             19473..19988
                     /locus_tag="PKL11_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ12153.1"
                     /translation="MNLLLQQSSAEVQQILAHYPAEHRRAAVMPLLFLAQRTTGRVDS
                     QALADIAEITGISSTDVASIMGFYTLFHAEEDGGGRFRVQVCTDMPCALRGSRQFLAA
                     FCEALGIQPGQTTPDGLITVEEVKCLAACDHAPFLQVQGDGELTDYPDQTVESALALV
                     ETLRQKPWARY"
     gene            20000..21241
                     /gene="nuoF"
                     /locus_tag="PKL11_00760"
     CDS             20000..21241
                     /gene="nuoF"
                     /locus_tag="PKL11_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNZ12154.1"
                     /translation="MTEYLLLRHRDLPDIDQLPIYRANGGFAAFEKALKTQQPVDVLN
                     TVKASGLRGRGGAGFPTGTKWSFLPNTIWPHYVVANADESEPGTFKDREILERNPFQF
                     LEGLMLACFAVQAQVGYIYLRGEFWPLAQKLNQRIAELEQTGLLGDHILGTDYTLRLY
                     THLGAGAYICGEETALLESIEGKLGQPRLRPPFPAVSGLFGKPTVINNVETLTNVPLI
                     VDRGADWFKTLGTEDSPGVKIFSLSGRVNRPGNYELPFGVTFRTLIDQYGGGILDGKA
                     IKAIMPAGASSTILIADDRTLDTPMDYKSVAAIGGQLGSASVIILDETVNMADLVNQT
                     VHFFKHESCGKCTPCREGTYWMAHITDRLAAGQPSPQDAALLGSVARQMQNKCLCALG
                     EFSTMAVVSALEKFPQDFGIS"
     gene            21255..23642
                     /gene="nuoG"
                     /locus_tag="PKL11_00765"
     CDS             21255..23642
                     /gene="nuoG"
                     /locus_tag="PKL11_00765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNZ12155.1"
                     /translation="MTKQITLTIDNQVITAPEGMLIVDAAKQAGVNIPVFCYHPKMEP
                     VGMCRMCLVDVGRPMVDRATGQTVLDADGRPKIGFGPKLETACTTPVSEGMVVITESE
                     KVKAARKDVLEFLLTSHPLDCPVCDKGGECPLQNLTLAHGPGESRFLYSDKQHLNKRV
                     PLGELIFLDRERCIQCGRCTRFQHDVVDDPVIGFYQRGRALEIVTLSEPGFDSIFSGN
                     TTDICPVGALTTADFRFGARPWEMKPAASICNHCPVGCNLTFNVRREAAANGRTVIKR
                     VLPRQNEQVNEIWICDKGRFVYQYTESPDRLTQPLIRKNGELVPASWDEALELAAQKL
                     KGLQPLLLAGGRLSNEALFNLRQVTESLGGQSCLYTHMSGGNLAVQAGLTAGSNLGGL
                     GKGDAILVVASDLHAEAPLWWLRVKQAAARGAALIVLTARPTRLDKFARFALRYAPGG
                     EGAALTALLSGEGEAAAAFAGAQNAIIFYGSDGLDLVGTERVARLSAELLEKTGHLGR
                     PNNGLVPVWPRANDQGAFELGYAPATDLPAALRSAAVAWIAAADPAGDAPALAEALDA
                     TDFVVVQELFLTETARRADIVFPAQAFTEQEGSFTSGERRVQRFYPAVPPQPDTRPDF
                     AIAAQVGQRLGLKLADSAVSLVFDQVAQVVPAFNGLTFPALAETAPQWPEIARSDLYY
                     GGTSYDNQQGLGVHLAAPKAPLHLPELPPAVPLPAGLVALPVTRLYDQGALIARTELL
                     RGRLSPAAVWMHPATAEQHGFSEGQPVEVLLAGCKVSRTVCLDDTLPQGLLIALR"
     gene            23654..24658
                     /gene="nuoH"
                     /locus_tag="PKL11_00770"
     CDS             23654..24658
                     /gene="nuoH"
                     /locus_tag="PKL11_00770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNZ12156.1"
                     /translation="MESGMLWEWIIKAAVLLLIMTGGFAYVTWYERKVLAHMQVRIGP
                     NRAGPLGLLQPVADGIKLIFKEELIPAGADKLLFILAPVITVIPALIVTAVIPWGETV
                     NLFGRPVALHIADVNVATLYIMAVTSISVYGITLAGWSSNNKYATLGGLRATAQMISY
                     ELALGLSFVGPIIIAGSMSMLDIVRTQQNTVWFVVLQPLAFIIYFIASIAEINRSPFD
                     MPEAEQELTAGYHTEYSGMKFALFFMAEYIKMIAISMIGASLFLGGFDGPFVDRLPGL
                     GPLWLFIKIVLWLFMLIWIRATLPRIRYDRLMAFGWKILFPLALLNVLITSIVVVLIG
                     "
     gene            24669..25157
                     /gene="nuoI"
                     /locus_tag="PKL11_00775"
     CDS             24669..25157
                     /gene="nuoI"
                     /locus_tag="PKL11_00775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNZ12157.1"
                     /translation="MLKGLLTTLKSLLEKPVTVQYPKEKRPVRPRFKGRHVLKRYDNG
                     LEKCIGCALCAAACPADAIFVEAAENTDAERYSPGERYSRVYEINLLRCIYCGFCEDA
                     CPTEAIVLGHDYELAFADRRDAVYTKDMLLEPAPQGAPGTPQRTPPGVFLRPIPDMKD
                     PE"
     gene            25168..25698
                     /locus_tag="PKL11_00780"
     CDS             25168..25698
                     /locus_tag="PKL11_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNZ12158.1"
                     /translation="MPVIQIVIFAILGLVAVGTAVGMLLSRNAVYAALFLVLNFATIA
                     ILYLILGAPFIALAQITVYAGAIMVLFLFVIMLLGVEKLPFKERLRGQRLIAILLGVV
                     FLGEAVLYLVYRGHAGQLPLPDAIQFVSPAELGRVLFSQYMLPFEITSIILLAALVGA
                     IVLTHHDKPTPAAEQI"
     gene            25709..26008
                     /gene="nuoK"
                     /locus_tag="PKL11_00785"
     CDS             25709..26008
                     /gene="nuoK"
                     /locus_tag="PKL11_00785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNZ12159.1"
                     /translation="MPITYYIVLSSILFVLGVIGVVVRRNAIVIFMSLELMFNAANLL
                     FVAFAAYYQIVTGQLFVFFVMTVAAAEVAVGLALMVAIFRAKHSIDVDQMSSLKG"
     gene            26012..27970
                     /gene="nuoL"
                     /locus_tag="PKL11_00790"
     CDS             26012..27970
                     /gene="nuoL"
                     /locus_tag="PKL11_00790"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNZ12160.1"
                     /translation="MDTLFALVPWVVFAPVLGILINLLVGKRLGEKAVGIIGSLASGA
                     AFIISVILAVALGQHPEGVVHPLAEWMTIGDFSVRWAFQVDTLSTTMMLVVSGVGTLI
                     HIYSIGYMHADTHHHGDPGRFRRFFVYLNLFIAMMMVLVTADSYLMLFVGWEGVGLCS
                     YLLIGFWYEKGEAGLGNARAGRKAFIVNRVGDFGLLIAMFVMFWAFGSLDFATVFAQA
                     GTVAAGALLAITFFMLLAVTGKSAQLPLFVWLPDAMAGPTPVSALIHAATMVTAGVYL
                     VARSQPLFALVPAAQTAVTWVGALTALFAATIAVGQYDIKKVLAYSTISQLGFMVAAV
                     GMGAVVGGMFHLITHAFFKALLFLAAGSVILGLERGHEAALNGQHLRHDHFDPQDMRN
                     MGGLRPRMRITYWVYLIGALALAGIAPLAGFFSKDEILAAASEQSLAVFIILAIAAFF
                     TAFYMGRQLILVFGGKPRSRAAEGAAENSPIITVPLIILAVLAALGGLLNLPGLHWLE
                     TWLEHSLAGLSAGHFILWVALLSLAVALLGFWLAWSIYARKPRQKPAEPDPLAAPLGD
                     IYRGMERKWWVDEFYRAVLLRPAAWLANLLAGPVDQGGIDGAVMGTGRLTRLAARGLS
                     LLQNGFVRGYALVMLLGVVAILTYLVLR"
     gene            28005..29498
                     /locus_tag="PKL11_00795"
     CDS             28005..29498
                     /locus_tag="PKL11_00795"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNZ12161.1"
                     /translation="MNWWTNPLVILIAFPLAGAALIALIGSRQTRLIRWLALAVTLIE
                     FGLSLWLLARFNPADGNLQLMVDQPWVDLFGLPVRLLLGVDGLSLLMVLLTTLLTPIA
                     ILSTWTAVEDRVKGFMLFFLLLESAMLGVFLALDLVLFFLFWELTLIPMYFLIGIWGG
                     ARRIMAAVKFFLFTMAGSILMLVAILYMTNAAGTAALPELMQQRALFSGAALWLFLAF
                     GIAFAIKVPMWPLHTWLPDAHVEAPTAGSVILAAVLLKMGAYGFLRFNLSLFPQTFVQ
                     LAPWIGGLAVVGILYGAAVAYAQKDLKKLVAYSSVSHLGFVMLGIAALNPQGLAGAVL
                     QMVNHGVSTGALFLFVGMLYERRHTRDLADFGGLWKVMPILGALTLIIMLSSVGLPGL
                     NGFVGEFNILLGAFGSSALGSFWFAALAALGVVLAAAYLLKMFEQTFLGPIKHEENRA
                     LLDLNPREIITLLPLLVLMFWIGLYPAPFLNLLNPTVTQILSLVAVP"
     gene            29511..30941
                     /locus_tag="PKL11_00800"
     CDS             29511..30941
                     /locus_tag="PKL11_00800"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNZ12162.1"
                     /translation="MIQTDLYVLLPVIIVIAWALMLLLVDLWVPKNRKGITAGLAAFG
                     LAIALGFSLSQSGSTLQGFSGMVVLDPFARFLNVLILGSGLAAIALAYDYLKRMGLER
                     GEYYTLLLISISGMMLMAQAHDLIIVFLALELLSIPLYVLAGFARPRLESEEAALKYF
                     LLGAFASGFVLYGVALIFGATGSTNLESIMGSIGSANPAMLLIGAGLLLVGFGFKVAA
                     VPFHMWAPDVYQGAPTPVSGFMSVGVKAAGLAALLRVFIVALPALAADLTPLLYALAI
                     LTMLVGNLLALAQTNLKRLLAYSSIANAGYLLMAFVPYGNKAMVAESNAAMLFFLAAY
                     ALGSLGAWAVVVSLERAEGRGLELDDYAGLGRKYPWLAIAMLIFMLSFTGAPLTLGFW
                     GKFYLFKVAVDAGFWPLALVGLLTSVISAYYYLRVVVVMYMQAGQPEAQGDRWVTLLA
                     VALAALLVLLSFFPGPLLNFAALAGL"
     gene            complement(31001..31786)
                     /locus_tag="PKL11_00805"
     CDS             complement(31001..31786)
                     /locus_tag="PKL11_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNZ12163.1"
                     /translation="MNSLFATERLKALLGFPFRDPKAGQKFLIGSLLIFAQFIIPILP
                     GIIVMGYLSQVLRRVTRGSGEATMPEWDNWGELFMDGLRVFGAIFVYSLPMILIVSVL
                     YFLMFAPLAAVPFLAEGNSDMAGLAIVLVLIAQLFMFLMIAVMMVVYIVYIILLPPMQ
                     VHTAAKNEFGAAFQIGKWWKIFRANFSGFMIALIIIIGVYALLMIGVQILYMTLVLCF
                     LIPFVTAPVTFYIGLITYALLGEVYRVGEEKAFPTEAVAEIAA"
     gene            complement(31973..33235)
                     /locus_tag="PKL11_00810"
     CDS             complement(31973..33235)
                     /locus_tag="PKL11_00810"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ12164.1"
                     /translation="MIDQNLPHVVILGAGFGGLRAARALAKRPVRVTLVDRNNYHLFQ
                     PLLYQVATAGISPDEIAYPVRAILRRQKNLEFRMAEAQRVNLAAKTLETNTGDMPYDY
                     LILAAGGQTNFFGLDAIARHGFGLKTLTDATNIRNHVLSCFERAAQEPNPDTRRALLT
                     FVVVGGGPTGVECAGAISELIRLVLSKDYPALNLKEVRILLLEAADRLVTHLPASLGE
                     ETARSLWQKHVEVRFGAAVENFDGMRVTLKGGEIIPTHTVIWGAGVQAASIMDTLGAE
                     QGAQRRVAVLPTLQLKEHPEVFVIGDAALLAGEDGRPLPMVAPVAMQQAETAADNLVR
                     QMKGEALQPFHYQDPGAMATIGRNQAVARLGKLEFHGFMAWMIWLVVHLMQLVGFRNR
                     LVVLINWAWDYLFYDRAVRLIEGERCQE"
     gene            33410..34390
                     /locus_tag="PKL11_00815"
     CDS             33410..34390
                     /locus_tag="PKL11_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ12165.1"
                     /translation="METKPTFKVRFWGVRGSYPTPGPETVRYGGNTPCVELEVNGRTI
                     ILDSGTGIIPLGRSLARRARERGGSLEATLLLSHLHHDHTQGFPFFVPVHFPNANLHL
                     YGPNLLGADPQMTLESVMQPPYFPLRLADLNARITFGTLRETDLLLIGEAVGGVTVRP
                     TSETAQYDHADVVQVRSLRSYAHPNGVMHYKISWNGLSVVYATDTEGYVNGDQRLAAF
                     ARGASLLIHDAQYTDEHYLGLLPGISITQGYGHSTVSMACQAAASAGAQRLALFHHAP
                     EYDDARLDQVAVQAQALFPDVLVAAEGLEIDLAAGLDMQTSKINLPLAVS"
     gene            34471..35187
                     /locus_tag="PKL11_00820"
     CDS             34471..35187
                     /locus_tag="PKL11_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ12166.1"
                     /translation="MSPVSPQTLAQIPLLNGLTPEQCDYLAARLHLRNFPGGVDIITA
                     GAEGQVIYILLQGTVKVYVPELNGNEVIVNILGPGDTVGEMSLVDRISRSASVITMEP
                     TTTLWMGQADFQAALETIPVLSQNLLRILSRRVRSSTAHIRALAALDVHGRVARQLLT
                     FAERYGQVNADGSATIRVRLTQGEISELVGASRKRVNQVMVAFRKAGWVTSDANYHIT
                     LLNQPALIRVSETAAIESYE"
     gene            complement(35189..36397)
                     /locus_tag="PKL11_00825"
     CDS             complement(35189..36397)
                     /locus_tag="PKL11_00825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HNZ12167.1"
                     /translation="MKRWILLIALIVLLLTGCTTAPQVGTPNIDRGSYLTGHTLTETA
                     AGEWETGFHLLPATDNEPQGILLNGSTNGNAVRLVIRDLDGQTVWEGEPCSGTFKYSV
                     VVDTLPAGEYALWLVWDGPVTAQLDLYIVPGEAVRLPEVPPLALLGGIGMTLVALGYV
                     IYAAVRRLGWGYLGLGALGWIVSVFLKFMVAIPANTPIYNLLIGETRGGIGEWAFHLW
                     VGLLTGIFEVLFIWLILRYTRVGKASWKKALAFGIGFGAFEALVLGLASAGSTLMAMV
                     SPGLLSTVNIAALAQNANPLWGLAPVSERFFVVLVHIFSNVLMFHAIATRKPGGLWIA
                     FIYKTLIDTLASYAQLAGLDTLTKIWTVEAIIAVWGVLGWLGTRWVSKHYPQQPEGEP
                     LPQPENQGPA"
     gene            36543..37220
                     /locus_tag="PKL11_00830"
     CDS             36543..37220
                     /locus_tag="PKL11_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ12168.1"
                     /translation="MKERILVVEDDDAIVRVLQRALSYEGYQVDAAGDGETGLKIALE
                     HRPDLVILDLMLPGMDGLEVCRRLRGVGNMPVLMLTAKDTVPDRIQGLDAGADDYMIK
                     PFDVDELLARVRALLRRTETERATILTFGDLVLDTSTRQASRLGRVIALSPKEYDLLE
                     LFLRHPRQVLTRDLIFDRVWGYDFGGESNVLDVYIRYLRQKLEQEGETRLLHTVRGVG
                     YVMRETA"
     gene            37220..38584
                     /locus_tag="PKL11_00835"
     CDS             37220..38584
                     /locus_tag="PKL11_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ12169.1"
                     /translation="MSLRTRLTLRQVAIFALGLVALCALVYALVSFVVLNKIDTLLAD
                     SAASLVDRMRLTEGDNFDIQSLRTVPLPEDLYLQVWGSDRLLQYSHPATFQIAMDTAG
                     WDLGRPAYTTVNRTGLQMRVLSVPLETARGPAGMLMVGFSLSLLTLARQTLLLIISVV
                     SVLLLCLMALSAYLSNSQMLTGLTKLTRLMPELIKMNDLSQRLPDTLLKNDEVGALSR
                     TFNAALDRLETLLTSQRRFMADVSHELRTPLTVIKGEVGLMRRMGKGDKESLHSIEAE
                     VDRLTRMVGDLLMLAQAESGRLPLNLSMINLDEVLMEVYQQALVLSPPGVLVRLLEME
                     PLVIEADRDRIKQVLLNLAGNALRYTASGGQVMLSLRSASGGVQIQVSDTGKGIPTDD
                     LPRIFERFYRGERSRKREKEGGLGLGLSISYLIIKAHGGRIDVTSQESVGTTFTIWLP
                     LTQR"
     gene            complement(38705..39460)
                     /locus_tag="PKL11_00840"
     CDS             complement(38705..39460)
                     /locus_tag="PKL11_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12170.1"
                     /translation="MEFSPTRSASDFLTGAFRRFRPGLLFGVLILSALLAFELFNYST
                     TDYALRDLLGNLQFAGIPWATILAIAFCGMDFAGIARLFTPEQGADEPKEVWYLFGAW
                     LLAATMNAVLTWWGVSMAIANHAVASVAVVDARVITQIVPIFVAVLVWIIRILVIGTL
                     STAGERIFSDMRKSSGYSRPRTTAPSASVPVGRPLTSTPAARPTASNPAPAARPYPRP
                     LSTATSSRPEPSYHSLSGQPVSRTSAGDPPARG"
     gene            complement(39562..40266)
                     /locus_tag="PKL11_00845"
     CDS             complement(39562..40266)
                     /locus_tag="PKL11_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNZ12171.1"
                     /translation="MKPAHTPTAKIETQWNKLFLFGGIAGLAMLGIMVAQITIFIIWP
                     PPETVPDFFVLFQKSWLLGLLSMDLLYLINNAVLILIYLALFMALKHTHPSAMLIALV
                     LGLVGIAAYYASNTAFEMLRLSQFYAEAADETQKAVFLGAGEAMLAIYKGTAFDVYYV
                     LNGIMLLITAAVMLKSELFSRATAIWGLAAGVLMSIPSTAGTIGLIFSLASLVPWAVF
                     LALVVRRFFKLAKLGS"
     gene            complement(40407..40838)
                     /locus_tag="PKL11_00850"
     CDS             complement(40407..40838)
                     /locus_tag="PKL11_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12172.1"
                     /translation="MEKKNTFTKVLAIAGTVLAWIPILFMVVTCVIGSIQSGEFLCDY
                     LIPAELMWVVFIGAALLIWAAIRARTQLKWIGWSLGIGLALLGGGLLTAQLTGLASGR
                     IANEGLPFVAVIAMLIGYDLAVIAMGIGGILLIKALYQHDK"
     gene            complement(40894..42150)
                     /locus_tag="PKL11_00855"
     CDS             complement(40894..42150)
                     /locus_tag="PKL11_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12173.1"
                     /translation="MKKLPAESIYYIRAATGELNSGLIFTSIWVLYYTVMQLSLIEVS
                     VLYIVLTVSNFILEIPTGIVADLYSRRLSVIIGGIFVGLAFMTVGIFPFFAIALLAGF
                     IEAIGDTCISGALQAWITDEVGEDNVGNVFLRGRQVATPAGWVGVILSIGLAAWMNYQ
                     APIILGGVLWLLLTIFLILYMPETNFKKNTEINRQSWRNQMKASSATFAEGIRLVRGS
                     TTLQALFWAFLLGSAFADGFYKFSRAHVLQSFTLPIITLPLLGALKDNLWFGVLEMLQ
                     GLFCLAGAEIIRRKIKLSDRGAAARTLLSLSAMTTVAVVIFTLTQHFGLAMATWVIVG
                     GLQNLSTPITEAWMNQNIPSKIRATLISMSSQAGMVGTLGISTGLGAFGDRFGVRRAL
                     GLSSIILLPMMLIYARKKDGAQNGCD"
     gene            complement(42131..42460)
                     /locus_tag="PKL11_00860"
     CDS             complement(42131..42460)
                     /locus_tag="PKL11_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HNZ12174.1"
                     /translation="MVKCKITVLKRGFNQDLVERYVEHERKKTLGPCEVFREGQEFIT
                     DVFGGIPAGFCPWAWDDIYKVLVGYAAGGSFGMWYEDGNCLIACCTDGTRPVYFKIEK
                     IDEEAAG"
     gene            42613..43719
                     /locus_tag="PKL11_00865"
     CDS             42613..43719
                     /locus_tag="PKL11_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ12175.1"
                     /translation="MDITALRAEFPVTDHVIYLNNAAESPLNNRVRQRLADYLILASE
                     APHKKPSVRHAVKAMLSDLFGGAADEYALVTSTGVGVSIVAAGYPWKPGDNVVLPADQ
                     HWNNTFPWLALRQRGVDVRLVPVDPDYRVNPQAIADRVDKNTRILATAAVEFNTGFRA
                     DLKQLSQIAHQAGALFMVDGIQAAGVCPLNIDADGIDILAAAGFKWLFGMPGTGFLYV
                     NRRAQDLIQPVMPGMFAADNNHTRDLLYFPDARRYETGTIAYSHFHSWTAGLELLKEL
                     GIVNIHARVLHLTDYLIAGLRARNIKITSPVEHIAERSAILSLTIGSPQENQALYEKL
                     FAQDIIVAPRDGRIRVSPNFFNTEAEIDRLLQAL"
     gene            complement(43729..44352)
                     /locus_tag="PKL11_00870"
     CDS             complement(43729..44352)
                     /locus_tag="PKL11_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12176.1"
                     /translation="MLQNQSRVTAIMLIGLGYVLKFISYSFLTAVVLIGFTGLVFIYI
                     NVVGTDMSVLKYFGFLLPLDANGSGSITEKDIMRVYGVLSMGLFGVSVLWDWLKRWIK
                     RSRAGSEPANVEVNVEGEGVQAPRRRLPNVLKRILISSVLITLIYVAVFVVIPSAPMA
                     TGENPLLLYPIMIIFLIIALIANVIFIGIDTAANKLINLGLSNLINQ"
     gene            complement(44374..44793)
                     /locus_tag="PKL11_00875"
     CDS             complement(44374..44793)
                     /locus_tag="PKL11_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12177.1"
                     /translation="MKYKWLRILIGVLTGFIALTAIGGGGALLTGMEGERFPLEWLQG
                     SIFKDYTVPGLLLAGVVGGSALLTCITIFRKLNAGIVAAMVAGLSMMGFIVGEFLILM
                     QVPPGPTPIELFYFGLGLVIFLLGGLLWIKEKQPAHH"
     gene            complement(44828..45322)
                     /locus_tag="PKL11_00880"
     CDS             complement(44828..45322)
                     /locus_tag="PKL11_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12178.1"
                     /translation="MKTFQEHMIEYKKLLQQGAVQAAYQGLMDYILALRTYFKNKYPD
                     FAVPSSIYPGYMDMTYFSLNPESFRQRGLKIAIVFLHEAFRFEVWLAGANKQVQAKYW
                     KLFQESGWAQYRLVPTIQGADSIIEHILVDNPDFSDLERLTGQIERETLTFIADVEPF
                     ITRH"
     gene            complement(45399..46055)
                     /locus_tag="PKL11_00885"
     CDS             complement(45399..46055)
                     /locus_tag="PKL11_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12179.1"
                     /translation="MDQTSTAANTSQIAIAKTMLSLENQLKGGVSWFFWIAGLSIINS
                     IIFFTGGSITFVVGLGATQFIDGVVSGVVNKVSSGTGTIIRVIGYGLDFLLAGTFFTC
                     GILGRKRLRWVVIGGMILYGLDGLLALAFGDWLSTIFHVLALFGLWKGQKAIKELGLL
                     EKNQSAGDLAAIQMMVKETQPVSNGAPRKNLVRFLLIVTVPFLILLGVLIVMILLNYK
                     "
     gene            complement(46226..47029)
                     /locus_tag="PKL11_00890"
     CDS             complement(46226..47029)
                     /locus_tag="PKL11_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12180.1"
                     /translation="MDVKSKVFVVTGGGNGIGRELVMLLLQKGARVAAVDISEKALQE
                     TVNLAGSQKDRLSTHVVNITDRAAVEALPAAVMAAHQAVDGLINCAGIIQKFVNFKEL
                     DYAEIDRVIDVNLYGTLFMTKVFLPYLMTRPEANITNVSSMGGFLPVPGQLLYGASKA
                     AVKLFTEGLNSEMAGTNVHVTIVFPGAIATNIAQNSGAHIDTGKSRPEKTMKTTPANV
                     AAEVIVKGIERNSYRVLVGSDAKMMDFLSRLAPKQAAGFINKAMGSLLQ"
     gene            complement(47217..47768)
                     /locus_tag="PKL11_00895"
     CDS             complement(47217..47768)
                     /locus_tag="PKL11_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12181.1"
                     /translation="MENTNPPAPAAPQYRIDENMVRAKIENYKHEQNLVLGIIGGAIG
                     GLIGAAAWAAITYFTEYQIGWIAIIVGFLVGFGVRTLGKGLDKIYGIIGGVIALGSVL
                     LGNFLASMGFLAKGLELSFLDTLLGFNYRLTFELLKETFSVIDLVFYGLAVYAGYRNS
                     FRNITKAQLLEGAVTRQDSGLPG"
     gene            complement(47821..48201)
                     /locus_tag="PKL11_00900"
     CDS             complement(47821..48201)
                     /locus_tag="PKL11_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12182.1"
                     /translation="MKIKRVTRLLIFTVICLVLGGCTTPAITEAQVEDLNENQFTIQT
                     NTQLTFQTLKIGVENIWAEDYSTRDGETVNGLTAALWLFVEDKSDLDQTVRVHSGMAL
                     EIDAYIINIVEVREDGIILEIISQ"
     gene            complement(48355..49002)
                     /locus_tag="PKL11_00905"
     CDS             complement(48355..49002)
                     /locus_tag="PKL11_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12183.1"
                     /translation="MNWDVSGLIIEGICCTGKTTLIQALTRSPRYNQKSYPSSVVISE
                     HHTQRVLERKQALEGLVPDDHIALLEFYVQFFEKIHERSSQMDWNARNRTNHRIPFLL
                     ERFHFTHVFHYGLDWELVRSIDERLAEINSKVCVLEINEADMERRIILERNEGWRKYL
                     QNFGRTNSEIVDYYQKQQALLLSLLQKTKLQWLKINTSETTVEGAVSQVLNFWDI"
     gene            complement(49044..49559)
                     /locus_tag="PKL11_00910"
     CDS             complement(49044..49559)
                     /locus_tag="PKL11_00910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNZ12184.1"
                     /translation="MPIENILIRDEREDDAEVITAVTAAAFETMEISDHTEQFIVKAL
                     RAAGALTVSLVAEVDGAVVGHIAFSPIAISDGTPDWYGLGPVSVLPKDQRMGIGKALI
                     WEGLARLKGLGAKGCCLVGHPEYYGRFGFENVTGLGLEGVPPEAFFALAFDGPMPQGS
                     VAFHEAFGARE"
     gene            complement(49609..50256)
                     /locus_tag="PKL11_00915"
     CDS             complement(49609..50256)
                     /locus_tag="PKL11_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12185.1"
                     /translation="MKKKLWLIPMLFIIVACGLLPPTTEDLDERVNATLTAIAQSNPP
                     ATVTPEAAVPTATQTAAPATAAPTLSLEQLRNGVYNSADWGQFQLTDGIYYRTPPTTQ
                     ESPEAYTTHMLDLVLFGDIDGDGVEDAIVFLTTQSGGTGHFVEMAAVLNQNGTANNVS
                     MLSLGDRVVVESGGIQAGLITLNLRVHGPNDGLCCPSQSATFTYRLDNQQLVQLP"
     gene            50363..51034
                     /gene="ung"
                     /locus_tag="PKL11_00920"
     CDS             50363..51034
                     /gene="ung"
                     /locus_tag="PKL11_00920"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNZ12186.1"
                     /translation="MPIPESWLPALSGETTQPYYQQLYRFVGEERRKYKIYPPGEDVF
                     SALRYTPLEQVRVVIIGQDPYHDENQAHGLAFSVRPGVTPPPSLINIYKELQTDLQFR
                     IPNHGCLIPWAEQGVLLLNAVLTVRAHQANSHQGKGWESFTDAVLRAVNAKTSRVVFL
                     LWGSYAQRKAEWIDANRHTVLKAPHPSPLSASRGFFGCRHFSKANAALQSAGLPLINW
                     QLPDM"
     gene            complement(51082..52248)
                     /locus_tag="PKL11_00925"
     CDS             complement(51082..52248)
                     /locus_tag="PKL11_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12187.1"
                     /translation="MINRVYLQQTLHKKLEANRVVALVGPRQCGKTTLAREFVPSDSV
                     NYFDLEDPLSLARLDQPMTALQNLSGLVVIDEVQRRPDLFPVLRVLADRTPLPVRFLI
                     LGSASPELLRQSSESLAGRISTLQMSGFSLQEVGVQAQSTLWRRGGFPLSFVAPDESS
                     SLEWRKDFIRTFLERDIPQFGFSIPSTSLFRFWSLLAHYHGQVWNATEAARTLNVSEN
                     TTRRYVDLLQDLYMVRLLQPWHANLGKRQVKSPKVYIRDTGLLHILLGIHTEQELLLH
                     PRSGASWEGFVIEEVIKAAAPDEVYFWGTHSGAELDLLLIKDGRRIGVECKRVDAPRL
                     TPSMRAAMQDLELSDLLVVYPGSKPYSLGEKIKAIPLSSLAGEWLSTGDELKDG"
     gene            complement(52447..53421)
                     /locus_tag="PKL11_00930"
     CDS             complement(52447..53421)
                     /locus_tag="PKL11_00930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12188.1"
                     /translation="MKPLPKYHIRPLTPDDQAFLWEMLYQSLYVPAGGDPLPRQIVRQ
                     PEIARYVENWGRVHDCGFIAEDESGKPLGAAWMRLFKGEEHGYGYINDATPEVGMAVL
                     PEYRGQGIGTELLMRLLAAAKGQYAYVSLSVGNGNPAAHLYERLGFEVVKAMGDSVIM
                     RKKMNPDSAHKSRWEIAEVVFGIPFLISLGVHAVAPLSVPPGGWRLGIIISGVVLIIA
                     AIGVVRLARRELRKYNQPTDPGQPTSRLVNTGVFRYSRNPLYLASVMLFLGLGLALNR
                     LWAVAALVLSIVICIQVLIRPEERYLAAKFGEDYQQYAASVHRWLGRR"
     gene            53574..53843
                     /locus_tag="PKL11_00935"
     CDS             53574..53843
                     /locus_tag="PKL11_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNZ12189.1"
                     /translation="MPVPPEGKRFYGAITVSERGQIVIPADARRDFNIQVGDKLLVLG
                     DLEQGLALIKASALLERSPDFFQFLLGSRPPEAPAQFPQPPEEAE"
     gene            53849..54691
                     /locus_tag="PKL11_00940"
     CDS             53849..54691
                     /locus_tag="PKL11_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12190.1"
                     /translation="MDALSVSHLEKTYPAFHLRDVSFSVQPGRIMGFIGRNGAGKTTT
                     MKSILNLVHPNAGSIQFFGLDMPAHEFEIKQRIACIFGAANYYSQSLLQTIADVYRRF
                     YPNWDDALYRTYLQHFALDPGKRLKQLSQGMSIKFALTLALSHHAELLILDEPTSGLD
                     PVSRDELLHIFEDIVDHEGASIFFSTHITSDLDKNADDILYIKDGQIIACEEKDAFVA
                     SYRKVDGLTADLPADLKTRIIGFHERKGEFSGLVRVTDARGVSGLNFTPADLESIMVC
                     TERG"
     gene            54695..55336
                     /locus_tag="PKL11_00945"
     CDS             54695..55336
                     /locus_tag="PKL11_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HNZ12191.1"
                     /translation="MKNLLYKEWKLAIHPTGYIFLLFGVMLLIPNYPYFVAFFYQTLA
                     IFFTFMNGNTTNDIFFTALLPVRKRDVVKARLGAVIGLEVLQMLIAVPFAILRSQIIP
                     TENLAGLDASLALFGLVFIMFGVFNVVFLPQFYRTGYKTGTPYLLATLVMTLVIVAAE
                     LVIQLTPALKQALDTPACTLPQIIVLFAGLLIFALLNLVAYRRSASAFERLDL"
     gene            complement(55413..55967)
                     /locus_tag="PKL11_00950"
     CDS             complement(55413..55967)
                     /locus_tag="PKL11_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNZ12192.1"
                     /translation="MFSDIQQLIWLFPIAFILHDFEEILLFEPWLKKNSGLIMSRIPN
                     KAPAFLAKQMPIILGKTTTQFAFPIFLIFVLTCISAFLAAEFQSYPIFLVASSLFFLH
                     GFMHVGQAIILRRYIPALITSVLIAIPYGIILFWKLLEAGVVSLWGLAIYFGVAIVLA
                     VPFIVGMHILGEAFYQRVMKILIG"
     gene            complement(55989..56537)
                     /locus_tag="PKL11_00955"
     CDS             complement(55989..56537)
                     /locus_tag="PKL11_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12193.1"
                     /translation="MAYLKLGETIADYSIGAVERNRLNPRILIFTEGTILGPERWWGW
                     LNDKRYAPIGKSADKISTWERQGARIAYLTSRRRLADVNAIREILGRGLFSGQYLFYR
                     GEHEQYRDIAEQVIPDILIEDDCRSIGGRWQMTITNVKDEVRQLIHSVVVREFMGIDH
                     LPDDLQELFLWNPTPASGTENR"
     gene            complement(56550..57821)
                     /locus_tag="PKL11_00960"
     CDS             complement(56550..57821)
                     /locus_tag="PKL11_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12194.1"
                     /translation="MKKHFLIFVVCLQLLLASCSPIAQENNADAMATDQDEWQCIGTV
                     DDFLYQMAKITRNDELYSLSDIYPSAPWELSAEIPETVYEEISNGLVTRVNIELVREY
                     SGQTELWLRSDDKIIKHNAGTENFSIISPFPYDQSGKTYLNVIVRDLFEISTGEIVGV
                     NYPQNNNTEWDQPIPVFSKYNEFENRFEFYDIGLPYWVDGAGYGNLSMSPQDDVIISK
                     SDEVIWIYQQQDGLYSYNPMDAELKHYETSFDGIVQSMVPSPDGFLLFYQEKQASWKL
                     LPGELAKYYPDSNTVEKIRVPLFRWPDYGTWLYTDSGDLWIGIHGYLSKDGTWVLKNP
                     NRLKYIHLGLNTESYNWAHPKLIFQSSNGYLWYTNENQDFLGVDGSAWYDPTSDKGCW
                     FTTGSGNIVEDNLKNLWMAIGDKIYKYSVSE"
     gene            complement(57903..58433)
                     /locus_tag="PKL11_00965"
     CDS             complement(57903..58433)
                     /locus_tag="PKL11_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12195.1"
                     /translation="MTPAIVIETPRLILRTVTMVDVDGVACSWKLDEGAISTAEAVLK
                     IEAMMAHYAQNRPGKIVHLCLAIIFKATNDFIGWCGLDHTNPKDADPALFYLLKSSYW
                     GQGLATEAAGALLNYAFGQLELASIHGGAAPDNLASKRVMEKIGMNYLGLDDEGGYAF
                     KITRDEYLTNSRKRRG"
     gene            complement(58441..59301)
                     /locus_tag="PKL11_00970"
     CDS             complement(58441..59301)
                     /locus_tag="PKL11_00970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNZ12196.1"
                     /translation="MHLRNLLSRRPLAAYIVLAFGLSWLLWWVAELGEKPVLFLDWLG
                     GFGPAFAAILLTGLLEGREALEMLLRRVFAWRVHPLLYLAAVTLPLIGTLLLIALYGL
                     INGKPETIKMIGPWLATLARTSPVLLMSLLLGWIVVTGEEIGWRGYLLPRLRARHSDL
                     AASLMVGLVWGLWHLPELWPFKAGSAPITLLFFMADIVIIAVLYTWLYANSRGSILLV
                     SLFHGVYDLMVMYASASLPFIRETRGLETVILLGMAAVVVAIYGPRRFEHGEQARNVP
                     DKVLIDAGPE"
     gene            complement(59375..60757)
                     /locus_tag="PKL11_00975"
     CDS             complement(59375..60757)
                     /locus_tag="PKL11_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12197.1"
                     /translation="MKKTAAFILTMLLLMILSTPTHADVAPPNPPPGSNINPSQDTKV
                     QMVAENVLMVVKEVSTDVYVIEVTADFAMKNLGETDEQMRVRFPLENAMGWGGDNYPV
                     ARYFKASVNQTSVPTEFVTETYLDDSFSPADWAVFDVNFPVGQVVNIRVSYTTDLQDG
                     FGVGDPDSFTEIQYFLGTGAGWYQSIGTVAITLRLPYTVCPATIDAYWPDHDNAHISE
                     IGHEIRWHWQNYEPNPREIIGVTIIHPNYWREILDMEARIGIDPGNVDLAIELSNKYK
                     RASFVKLGNPPGQLSHLAEITIQQALVLHPDDIRLHLELLEMYYDWYLWDYYYMSDDT
                     SLENSKQLLNYEMGVVLALDPTNQRIHEIQAEIANAKEEDRWSVLYPTPSATPLVCPS
                     ATPTRTIVAKTPTPKPSETPVPMITPTGTPEVSQKSNPIGGQVLAGVVMLVLGFIAGV
                     VFMKNRARPV"
     gene            complement(60965..61441)
                     /locus_tag="PKL11_00980"
     CDS             complement(60965..61441)
                     /locus_tag="PKL11_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ12198.1"
                     /translation="MTETQNSNSLCNEEPKNRIPLEVGAMTPEQIAMIFTHLPVDVTF
                     VDEGNDVRFYSGGHNRVFERTPDIIGRNVLNCHHPDSVQIVFKILEDFRAGTRDTAEF
                     WIQTKPQEGQESQFIHIRYFAVRDPQGKFLGTLEVTQNVTEIRKLQGERRLLDEVK"
     gene            complement(61552..62406)
                     /locus_tag="PKL11_00985"
     CDS             complement(61552..62406)
                     /locus_tag="PKL11_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ12199.1"
                     /translation="MIITFLGTAAANAFPEAFCKCKNCEQARLLGGSSLRKRSSLLVN
                     DDLLIDLNPDIMAAAQNHGCTLTNVRYCLQTHSHADHFDLSHLLSRSPEYGTLGTPIL
                     NFYASAETLEKAAEIFERDLADYSLLSPEAEVRLKLKLHQIEPLQPFSMGNYRIIAFP
                     ANHAPGMGALLFAVEENGRSIFYGTDTASLFEETWQAFHQFQMKFDLVILDHTYGPSQ
                     PGSDHLSAHQVIEHVQRMRSEGLLKSNGRAFATHIAHEGNPAHPELAAFAAEHGYEVA
                     YDGLILEV"
     gene            complement(62445..62645)
                     /locus_tag="PKL11_00990"
     CDS             complement(62445..62645)
                     /locus_tag="PKL11_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12200.1"
                     /translation="MGPVQQIRVLRGGDNHCYQIVTPEHEVVLKEMSINAMNHPKMEP
                     VVAETLNSAANPTPRFYPPIGC"
BASE COUNT        13327 a        19396 c        15723 g        14288 t
ORIGIN      
        1 gcaccacctc ggcggggctg ctttctgctg tgagcagcag ttcggccccc agcttcggcg
       61 agagtgagtg gatgatgtac agcatctcag cctggacatc ggcgtccatt tcgacggcaa
      121 acgcactgcg cagcgcctca tctgcttgtg gatgctgcat ccatgccagc ggccaggtgg
      181 cgcccagccg gactgccggg tgagcatcat ggagtgctgc caacagagcg ggcagcaggg
      241 ttgccagcac gtctggcgcc gataattcga tccccagata attgctggcg tggaataacg
      301 cccgcagcag cggctgacgg ttttctccgc gggcttgctc aatccgggtg gcataatctt
      361 tcaggcaggc cgtgccgcac agttccagcc agctcaaccc ggccgcgtag cgtaccgcca
      421 gtaccgggtc atccaggctg tgcagtacca gtgcaatccc ctgtggaatt tccatcaatc
      481 ccagggtttc cagtgcttcc acccgtacat cttcatcttc ggctttcagc acctgttcca
      541 ataccagcac agcagccggg tcgctgtgtg tccgcagtcc ccaggcggct gccgcccgca
      601 ccaggtcgtt ttcctctgcc aacgcttcaa tcagcatctg ataagccgcc ggctgccggc
      661 agtcgcacag ggcatagacc gccgcctcgc gcacccgggc atcttgatcc tgcagcgctt
      721 ttttgagcat gggccgggca gtgtaccatg aaaaaccata cagataccgt gccgcactgg
      781 cccgcgccgg aaccgcgccg ctttgcaagg tgcgctgcat ggcctgtcgg gcgggctggt
      841 cgctgcggcg catcaataaa cgggccgcgt tgtaccgcac ataataatcc ttgtgattaa
      901 gcgccgcttc ggccgcctca atggatactg cctccggtgc ggctgtattt aagacaatcc
      961 ccagattctg caccgaaagc cgccaccctc ccgcttcttt ggggcggttg agagaaaaca
     1021 tgggttcgcg tccggtgata tcggtcttat tcatgcctgg ctcctcagcg cagccgcgtc
     1081 agcgggtgat tttccagcag cattttgccg gtttgtaaat cggtgacgct caacttgagc
     1141 agcccatccg cctcaattga aaagctggct ttcagacggt cttcacctgg tttgcccggc
     1201 ggcgtaagcg gcacacacac gttgccttcc aatgtttgca ccgccagttc atcatcccca
     1261 gtaacaaaca ccggaacgcc accctcatac cgcacatcca ctttctcaat ggtttcgctg
     1321 tcaatttccc caatcacaaa ctcaatttcc tgctgcccgg ggtgtgccgc gcacagtgtc
     1381 aattcaaatg gtttcggggt ggggcagggt gtccccattg ggatgatttc actgaagctg
     1441 ggtgtgccgt tttctaagtg ccgcagacca aagctgtgtg tcaggtaatc ttccagtgaa
     1501 gcgccgcgcg ccagttgcaa tgcgccttca gctaccgccg tgaagggttt atcacaccgg
     1561 acgatccctt ccgtaaagta gcgatgcatc aactgctgca ccgagggcat caaagacgtg
     1621 cccccaatca tcaacacaaa ccggatatcc tcgcggtaaa taccccgctg ccgtgccagg
     1681 tacattgtct tttcaaccac ccggcgcatg gcggcataaa acccatgcgc ctcaagcacg
     1741 tcttccagct ctgcccgggt gaactgcaaa tggtgagtct gctccccgat gttaaacgcc
     1801 agctcagccc gttcctggct tgatagctga atcttgagcg cctcacaggc ggtcaagagc
     1861 ggcgtttcat cgtcgctcag gctgtctgca ttgatcccaa tctggcgcag agcttcctct
     1921 gccagccagt gatccacgtc actgccgccc agcatttgcc cggatttcgc cagcactcgc
     1981 gccgtgcagg tggaggtccc tttcaacccc agcagggttc gcagcacacc tcccaccttt
     2041 gagccacttt cgggcagctg caccaccgag aggtccaggg ttccgccgcc aaagtccacc
     2101 accagcacca tggcgccggg ctcggtcacg gcatagccca gtgccgccgc agtcgattcg
     2161 tcaatcaccc ggatctgttc ccggctcagg ttcccgactg actcatccag ccaggtaatg
     2221 tattgctcaa aagcaaccac cggtgtcgca atcaccagcg tttccagctc gctgattggg
     2281 aaaggcagcg cccccagcac ccggctgaga aaggcccgcc cggcgtccca atcagtccag
     2341 cgattgccgt caatcacccg ctttgattcg ggctgaggcg ccacaatccc gcgcttgaag
     2401 ttgcggaaca gccgttgtgt tcgctgccgg tccagcccgc gctgtcgcac ggcatgccca
     2461 accaccactt cctcgctgtc tacataaacc agcgaaggta ccagtgccgg ctgagaactc
     2521 tccgctggct ggctcagacc cggcagcata actgtctcag cgtagtttaa gtccgcgttc
     2581 caacgcgcca gcaccgtatt ggtggtgcca aaatcaattg ctaactgatg gggcatgaaa
     2641 aaatccggaa aaattcaggt tgaaaaatct tgttaattgt atcataacgg gtaaaattga
     2701 cctcatgccc gctgtctcta atttgaccgt tcatccctat ggcgattcgg ctgtgattgt
     2761 ttatctcggc agccagctcg accgcaatgt taatcaacag gttcatgccc tggcgtcccg
     2821 ggtgatgcag gcgcagcttc ccggcatttg tgaggttgtg cccggatatg tttcccttgt
     2881 gatccatttc gacccgctgc aaacctcact gcctgctgtc atggatggtg tgaacgattt
     2941 acacgcctca tcggggcagc agccgctccc tccagcgcgc cgtgttgaaa ttccggtgcg
     3001 ttatggcggt gaagggggcc ctgacctcga ttttgtcgcc cgtcatgccg ggctgaccac
     3061 tgccgaagtc atccgccgcc atgccgcggc tgagtatacg gtctatatga tgggctttct
     3121 gccgggtttc ccgtatcttg gcggtctgga tgtaagcatt gccgcgccgc gcctcgaaac
     3181 cccacgccgc cgcgttccag tgggttcggt tggcattgcc ggtgaacaga cgggcatcta
     3241 tccgctcgcg tcccccggcg gctggcgtct gattgggcgc accgaagttg ccttatttga
     3301 cctgaccgcc catccaccct gcctgctggc gccgggcgat caggtgcagt tcatccccat
     3361 ccgttgaccc cgccctcact catccgaggt gggagcaaga caggccattg ccgcaaagag
     3421 cagcccaacc acagccgcag caagataaat aacagccata ttgttccacc tccatttctt
     3481 agaaatctct acaagcatta acccagatca acccaaaagg ttgccctgat aagacgctgg
     3541 cgcatccacc ggggttcccg ttggcgtgtg ctataatccc agcacatgcg cacgttcaat
     3601 cgcatttcaa tcccgatttt ggccgcgttg attttattgg ccctgtgggg gtgtgggccg
     3661 caaacgccgc ttcccccatc gcacagtgtc accccgacag ctacgccact aattgcgtat
     3721 agtcttacgc ctgcgccgga attgccatct accacgccaa ccccggcggg cgctctgccc
     3781 atctgggcgc cgtttgctgc tcctcgtcaa acgcccgtca cgcccatccc cgcgccgctc
     3841 accggtctct ccctcccgga tgaagtccgc gtgctggctg tcctcggcat ggatcagccc
     3901 gcgccttacc cgggtcgcac cgatgccatc ctgctcatca tttatcaccc gcgtcttgcc
     3961 aaggccagcc tgctatccgt cccacccgat ctgttgggct acgttccggg tcagaccatg
     4021 cagcgcctga acactgccta tgccgtgggt gattttcgca cgatttccga tgccttggcc
     4081 tacaacctgg gtctgcgccc cgatgactgg ctggtgattt acgaggatgc cctgatctgg
     4141 tttgtagatg atatcggcgg cctggaaatc accctgctcg agcctttgcc gccccatgcc
     4201 tgcggcggca tccccgctgg tccgcaggtc ttgaatggcg aacagaccct gtgctacctg
     4261 cgttatcggg atggcagcga cgagctggcg cgcaattttc gtcagcagga agttgcccgc
     4321 atcctcttca tgggtctgtt ggagagcggt tcgctcgtca acctgcctga tttatattcc
     4381 gattaccgcc catttatcga aaccagcctc tcgcttgaag acctgctgct ggccgttcct
     4441 ctggctgtcc aactgggcga tgaaaaccgg attggctatt tccagatcca acctgctgat
     4501 ctaaccccct gggaaatttc cggacccatc cccgcgccgg tagtcttgtc cattccagac
     4561 gcggtgcgcc agcgcatcca gcaggccatc aattttgtgc tgacccccga accgttcacc
     4621 aatactgtgc tgaccctcga agccgccctg acggttgccc ccacgcccac gcagaccccc
     4681 accctgaccc tgacgcccac cccgacggtc acctatacgc ccacgatcac actgaccccc
     4741 accgaaaccc tgactcccag catcacgcct acccccacgc agacgttcac cccctcgcca
     4801 acggcaaccc ccacgccctg acggctactg cccggcctct acaaacagcg ccacctgcgc
     4861 aatctgacca tccagcgcgt aaacggtgaa cgcgaactcc gcctcatctc ccgtggagag
     4921 cggtgccggg ctgtcccacc agcgcaggcc gatcaccgcc ccggcttcat cataggccac
     4981 cgccagcacc cgacaccagc gcgcttccac agctccctcg ctcaacgccg cctgtccgct
     5041 gactgcggcc cacttcccat tgatgtcaat ggttgtggtc gggttcacga tctgcactgg
     5101 catatagcgc gggtcgtcgg ccgccagcgg cagtgcactg acgagctgca aagccggttt
     5161 tccggcaggt aaggcattgc taaagtgcgc cgccagcggc agcgcctgcc cggccggcag
     5221 cacattcagc ggcagggtgg cagtttctgc cgcttcctgc ccgctttgcg gatcggtgaa
     5281 ctgcatcagc accgccacat tctccagccg gctctcgctg gatgccgtca caaaacacca
     5341 ccagccgccc ccggcatcgg gcaggcaggc tggttcgccc agttgcagcg gcgcggcagt
     5401 tggggtgggg gtggcctcgc ccggcggcgg cggtgtcact tcgggcagca gcagcaccgt
     5461 gccaatgctc ataatccgcg ggtcaacggt tgggttcaac gtcttgatga cttccacggg
     5521 tacgccgtag cgcagcgcaa tcccgaacat atcttcattg gccttcacca catgcgtgcg
     5581 cggtgttggg gtgggggtgg gcagcggcgt ggccgtatag gggctggtgg gtgtggctga
     5641 tggcagcggc gtggcagaag gcagcggcgt gaacgccccc gcccgcgtgg gctgcgccgt
     5701 tggggttggt gtggcagtac agccagccag caccatggcc agtatcaaaa tgcccagctt
     5761 tacataggat ttcatgctca aaattatacc attcccccgc cggtcaccct caccgtcaaa
     5821 tgcggccaaa ttttgcccaa catttgccca acatttgccg ggtttatgct atcatcaagt
     5881 catccttaca gatgggagtc agcaatcatg gaagcattcc tgggactgtt ggttggggcg
     5941 ctgggctatg ggctggttga aattgctaaa aaaaccgccg accttgtcat tgaccccgct
     6001 cttgagccgg ccaaaaatca gatcaaggcc tggcttcaga aaaatttcac ccagcagcaa
     6061 cgtgatgacc gcctgaaaac caccatcctc caggcgctga cccaactatc cctgcccaaa
     6121 gatgaagacc ccctgcgcgc ctgggcccag caaaccgccc tcgaccgcct gaccgccccc
     6181 gccaatgccg ccctgcgccg ccagtttgcc ctggcagttc tggcccacac caacccccat
     6241 gccgaacccc cgcccgacct gctgctggcc ctccgctggc cgctgccccg ccgcgccgaa
     6301 ctgaccagcc tgctcaccgc tctgcgccag aaactggccg acaaccccga ctggcagccc
     6361 ctgctccact actttactga ggccgcccaa ttaaacctgc tcaccggcct gctctcggct
     6421 gttcaaaaac tgctggatgc cgtcatccag accccctctg gcccggccct gctcaccgtc
     6481 tcgggtcagc cggtgatgcc cgaggccgat ttctggcaga tccaggcggg ttattgcgat
     6541 aaggtgaaaa aatgctggtc cgaacatctg atctccggcc tggcccccga ggccggccgc
     6601 ccggttcaaa tgccgctggc cgaaatgtat tttgaacccg ccctgctgcc cgcccgcgac
     6661 ccccgcgagc ggattgaaga agagcagaaa atgctcaccc gcgatgccct cgaacgcctc
     6721 aatcacgaaa tggcccgcgg ccgcgaccgc ctggtcgaag cctttgggca gcacccccgg
     6781 ttggtactgc tgggcaaacc cggttccggc aaaaccatca ccctgcacta caccgccctg
     6841 atgctcgccc agggtgaaag cggcgcggcc cgcctgggcc tgcccgccag cgccctgccc
     6901 atcctcatcc gcctggccga ctacgccatc aacctcacca ccaaccccgg ccaaaccctg
     6961 catcaagccc tgactgaaga atttctcaaa ctctgcccct acgtcgaaca tgccagcctc
     7021 ttcctggaaa aagccctcca gcagggccgc tgcctgatcc tgctggacgg cctggatgaa
     7081 gtcggcgact ttggccaaaa attaatcgat ggccaaaccc ttcaccaggt agtactcaaa
     7141 cgcatccgcg aatttgaaca gctttactgc cgctccgaat cctgcaaccg catcctgatc
     7201 accagccgcc tcgaaggcta tgcccccggc agcctgcctg gctttgccga agccgaactc
     7261 agcccgctga gtttccccga cgaggttcaa acctttctcg tctactggtt caccgcctac
     7321 cgacagcgca ccctctccct cacctgggat caagcccgct accaggcggc ccagagtgtg
     7381 caggacgatc tgatgcccaa aattgccgcc tcgccgggcg tcaccctcct ggccaccaac
     7441 ccgctcctgc tcaccatcct ggccctgctg tttgccatgg gcaagcgcct gcccaaccag
     7501 cgggtagaac tgtacaaaat ggtggtcatg accatgattg aaaactggcg ccatgcccag
     7561 accgatcacg ccagccgtct ctttgaccac atccaacccc gcgatatcct ccgcgtcctg
     7621 gccgagctgt ctttctggct gcacagccag ctccccggcg gcaccatgat ggctgaccag
     7681 tggcaggccc agacccacag ccagttgctc aaaaacgacc tctgcctccc taaagaggcc
     7741 gccgccctga ccgaagactt tttagattac gcccgccgcg aaaacggcct gctgaccgag
     7801 cgcagcgccg ggcgctttgg cttcttccac ctcaccctcg aagaatacct ggccgcctgg
     7861 aagatcgccc acaagcccca ggaagaacgc cagcgcattt tagtggagcg ctggcccgac
     7921 ccctactggg aagaagtgct cctgctcacc gccggcgaac tgcaagacgg cgaagaagat
     7981 gccctcaaaa acttcctgct ctccattctg gccctcgaat ccaccgaccc ccaactggcc
     8041 ggccgcccgg cgctgctggc cggccgcgcc ctggccgata ttcaaaatcg caaacgcagc
     8101 ctcaatgaac tggtcatccc ggccttgcaa gcggttgccc aggatactga tccaatttcc
     8161 cgcctcccgc acactcccgg ccgggtgccc gttgccaccc gcgccgccgc cgccgaggtc
     8221 tgcgatgaac tgggctggct gccccccgac ctcgatgact tcatccccct ccccgcagcc
     8281 aacctccggg ttggcaaata ccccgtcacc aacagccaat atgcccgctt tctctttgcc
     8341 gatgattttg cccacccacc ccattggacc gacttccccc agttcgatga acaatcccgc
     8401 cccatgggtc catccaccga cgcggccggc ctccgctggc tggaaaccgc ccggcaagac
     8461 cccgacctgt cgcccgatgg caaacgagtc ttgccccgct actggaccga cccccgcctt
     8521 ggcgcttccc gcccgggcgc gcccgtggtg ggcctcacct ggtacgaagc caacgcctac
     8581 tgccagtggc tggccgccca ctgggccaac cccgccctgc atggcggcca ggcccccggc
     8641 tggggcatcg cctcggctcg cctgctgacc gaagccgact accgccaggc cgccagcgcc
     8701 gctcaatatc cggccctcga ccgcttcccg tgggatgctg aaacccccac cacagaccag
     8761 gctgaaatcc tgcgccgtgc caacctgggc cccgccgacc tcaaccgcac caccccggtc
     8821 tggatgtacc ccgccggcca gagcctgctg cacatctttg acctggccgg caactgctgg
     8881 gaatggcagg ccaattatta tgacaagaac cacaacctgc ctgcgctgcg cggcgcggcc
     8941 ttcctcgatg gtgtaggcct cgcgcggctt tcggggcgcc tctggggcag cccgctcagc
     9001 agggaggggg ctcgtcggct ttcgggtttg cctggtctgc ccataactct gggcgctggc
     9061 actctgcgct ctggcactct ggcccgcccc caattttcac ggcagccggc ataattcttg
     9121 caactattcc aacatgccaa ctcccgaaat gcccatcttc acccgcacct tcgatttcct
     9181 cacctggctc ctgcccacca cccagtcctt cccccgcgcc caccgtcaaa gcttcaccct
     9241 ccggctgctc ggcgccgcct ttgaactcag cgaccagttg gaagttgcca accggcgcag
     9301 cggcgcagag cgccgccacg ccctggccct ggccgatgaa gccctcgacc gcgtgcgggt
     9361 ctacctgcgg ctggccgtcc gctggcattg gctgaccccc ggccagtacc ggcatgtcgc
     9421 cgtcatgacg gttgaaattg gcaaactgct cggcggttgg atcaaaacag ttcattaaca
     9481 tctgtggcaa atcgcggccc tgggccgctt tgcggggacc cgcccaaaga caagcctgcg
     9541 ctgcgcggcg cggccttcaa caataatgta gacaacgcgc ggctttcgag gcgcaacagg
     9601 aacaacccga acaacaggaa caggaacatc ggctttcggg tttgcctggt ctgcacactc
     9661 ttttttatgg cctctttcat cctgtttgaa taaaggcgct gcccggtatt gcggtgcggt
     9721 cacggcttca ccgccgagga aaagaatggt ggggcaggtt cctggccgcg ctggcttctg
     9781 ccagccgggc gaataacaac cgtcccgcgc tcccggtgcg tcccgagcgc gggacaaatt
     9841 tgctcccacc ccctgatctt tatgtatgct caaatttgtt cctgggataa tttatggctc
     9901 gcttttcaaa aggccagccg cggcaaacgc tcgcgcccgg ctgccgcagc ttttgaacac
     9961 cgcctggaag acaacctgct ggacctgcag gccgatttac tgaactttac ctacaccccc
    10021 ggtccgtatg acagcttcta cattcatgaa cccaaacggc gcttaatttc tgccgctccc
    10081 tttcgcgacc gcgtcgtcca ccatgtgctt tgcaacctca tcgaacctat ttttgagcgc
    10141 agctttattt ttgacagcta tgccaatcgg gtgggcaaag gcacccatcg cgccctcaac
    10201 cgcgcccagc agtatgcccg ccgctatccc tatgtgttgc aatgcgacat tcggcagtac
    10261 ttcccctgca tagaccacgc cattttacag aacatcctgg cgcaaaaact taaagatccg
    10321 gcggtgctct ggttatgctc ccgcatcatc gccagcgggg caagcgtgct gagtgaaacc
    10381 cccgagcagg tgtactatcc ccaggatgac ctgttcgccg cgctgcgccc gcgcggcctg
    10441 cccattggca atctcaccag ccagttctgg gccaatgtct atttgaacgg ctttgaccat
    10501 ttcgtcaaac agaccttgcg ttgtcccgcc tacatccgct atgtggatga ttttctgttg
    10561 tttgcccata ccaaagccgc cctttggcaa taccggtctg ccctgcagca gcggctggcg
    10621 cagctccgcc tgtccatcca tgccggtgca caccctttcc ccgtcactga gggccttccg
    10681 tttctgggct tcatcatcca tccggcttgg cggcggctaa aacgacgcaa gggcctttat
    10741 ttttgccgtc aatatcggcg caaacagcag cagtaccagc agggcctgat tgccctgtca
    10801 accctgaccg ccagcgccca gggttggatc aatcatctgc gctatgccaa cacaacaggt
    10861 ttgcgccagg ccgtttttca aaccccaatt ccacccccaa accgccaaac cctgcccccc
    10921 actgcctgaa tcctaaatct cctaccccgc cgccttcacc gtaaagctgt caaaatcgcg
    10981 cgccaccacc gcagttggga ataccgattg cgcctcagcc agaatatcct tctcgcggta
    11041 gcgccgggag acatgcgtca ggtacagcgc gcccaccccg gctttcagcg ccagctcggc
    11101 ggcgcgccgc gccgtcaggt ggttgaactg ccgggccatg tcggcttctt catccaaata
    11161 ggtcgcttca atcaccaacc catctgcccc acggcagatc tcaaccagcg agtcggtctc
    11221 tccggtatcg cccaccacca ccaaccgtgt gccaggcttg aagtctccca gcacggtttc
    11281 cggctcaatc gtccgtccat ccggcagggt gatggcctgc cccgccgcca ggtcgcgccg
    11341 ccacgggcct gctggaatgc ccagcgcctc ggctttttct ggtaaaaaag gccgccgccc
    11401 cggttcctca aacacaaacc ccaggcagtc gggcccgcgg tgctgcaccg gaaacgccga
    11461 aatactgaaa ccatccaccg accagaacgt gccccgctcc accgcctgca tctgaatggg
    11521 cattggcgcc tgcgcgccgc gcagcaccac cccatacagc aggtcgtaaa ttcgctccag
    11581 cgccgcccgg cctccgtaga tggtcagctc gtcaatcgct tcccagcgca gataggtgga
    11641 caataacccc gccagcccca ggatgtgatc caggtgcccg tgcgtaatca aaatctgatt
    11701 gagccgcttg aacccgatcc ccgactgcaa aatctgccgc tgggtcccct cgccgcaatc
    11761 taccagaaac cggtgctcat cgtgcttgac caccagcgca ggcaggctgc gttttgccga
    11821 tggggccgag gccgaagtgc cgagaaaaac aagttcaaac aagcggttct atcctttcca
    11881 atgccgggtt tcagcgtccg ttgattttac cctatcttcg ttctttttgg gtttttcgtg
    11941 tacaataatc ccagattcaa agtttcagga gtggaatcta tggaactcat ccctgttcaa
    12001 attcaaaaac cggaagaaat taattttatc ctcggtcagt cgcacttcat taaaacagtc
    12061 gaggatctac acgaagccct ggtcaatgcc gtccccggca ttcaatttgg cctggcattt
    12121 tgtgaagcct ccggcgagcg cctggtgcgc tggagcggca ccaacgccga aatgattgaa
    12181 ctggcccgcc agaacgccct ggcaattggc gccgggcaca gctttattat tttcctcgcc
    12241 cccggttttt atcccatcaa tgtcctcaaa gcagtccaga acgcccccga agtctgccgt
    12301 attttctgcg ccactgccaa ccccaccacg gtgattgtcg cccaactggg cgctggtcgg
    12361 ggcatcctcg gtgtggtgga tggcgaccaa cccattgggg ttgaggatga agccggcatc
    12421 gcctggcgca cgcaattctt acgccgcatt ggctacaaac tgggctaggg aactttcatc
    12481 ccgggcccgg cgtcatttcg tcagacggaa tttgctccgt cattcatcaa ggagaaaaga
    12541 gaaatgaagc gtattatccc cctgttgctg gtgttcagcc tgcttctggc tggctgcagc
    12601 attttgaact ttggtcaggc ccaacccacc ccctcggatg ccgatatggc cactcaggtc
    12661 gccctcgtcc tgaccaacca gcccagcccc acctccccgg cagatgtgcc cacccagcag
    12721 attgaagccc tgcctacggt ggccctgccc acgcccacca ttaacatccc ggctacggca
    12781 acggtgcagc ccaaaccgac cgatggcggt ggcgcaccca cccccactgc tttgagcatc
    12841 tctcccacgc cgcttgttca acccaccgat acccccacgc cgctgcccac ccccaccttc
    12901 cccaccaatg atccccgcca gacgctgggc aatcccacct tcaccgacaa aatggataac
    12961 agcgattact ggcccaccgg cgaagatacc tacaccgcgg tcaccttccg cgatggcaaa
    13021 atgctgctga ccgccctcac cgaaacggat ggctggcggc tcatcactta cggccccacc
    13081 gccaacttct atgtcgaaat caccgcccgt tttgaggcct gctccggcac cgatcacttc
    13141 ggcttctatg cccgcgtccc cgagaaaaat cccgctaccc gtggctacct gatcggcatc
    13201 aactgccttg gccaataccg ctttgccgag tgggatggca ccaccgatcc cggcacctgg
    13261 accacccacg tctattggac caccaacgcc gccattctgg cgggtgccaa ccagaccaac
    13321 cgcattggtc tgttggctga aaataaacac ctcaccctgt atgtcaacgg cgtcaaggtc
    13381 ggcgagatga cggatgagag ctatcagagc ggctacctcg gcctctttgt tggctccgat
    13441 gaaacccaaa acctcaccgt ccaggttgac gaatttaatt tctgggctcg ctaatttttt
    13501 gaccttttca gcggccttgt cgggttcccc ggcagggccg cttcaggccg cctgatgagg
    13561 taataaccca tggtcaccgc tccctctgtt tctgattttt gtcagccggg ccccatgaca
    13621 gatctgggcc cctaccgccc gctgattgac gccctgcctg tagatctccc cgccttggtg
    13681 gactgcctcc agcacctggt agtccacatt ttctgggcca aacgatatgg tctgcaatta
    13741 accgaagacc gtgaaaaaga agtccagctt cgcccggctc aagccaaact ggcccgcctg
    13801 ctcgaactgg atgcccgccc gctgaacgaa ccgcgcccgc tggatcgccg cctggtctgc
    13861 aactgccggg atatcaccac cctgctgtgc gccattcgcc gcgcccaggg ctatcctgcc
    13921 cgggcccgct gcggcttcgc cacctacttt atccccaacc attacgaaga tcattgggtc
    13981 gccgaggtgt gggatgcatc tgccgcgcgc tgggtgctgg cagatgccca gttagatact
    14041 ctgcagcgtg aagtgctggg catcactttt gatccactca acctgccgcc ggggcagttt
    14101 atcaacggcg gcgcggcctg gcagctctgc cagaccggtc aggctgaccc cgacaacttt
    14161 ggcatttttg agtggcacgg ctgggatttt gtgcgtggca atgtcttgcg cgactgcttg
    14221 gccctcaccc gtgttgaagt gctgccctgg gatttttggg gcctcctcaa caccccctgg
    14281 gctgagtcat ctcccgctga ccgcaaactg gtcgatgacc tggctgcggc ctctgccgcc
    14341 ggagatgcct ccgctgttca agcttacctt gctgttgacc cgcgcctgcc cttccccagc
    14401 gcggtgtaaa tccattctct ggagctcaca tgactgctgt tgcaatccgt acccaaaatt
    14461 tgacgcgcac ctttgaaacc gtgcgcgccg tggatggaat ttcttttgaa gttccctccg
    14521 gcatcgtctt tggttttctg ggccccaacg gctcgggtaa aaccaccacc atccgcctgc
    14581 tgcttggcct gctggagcca acctctggcg gcgccgaagt cctcggtttt gataccgcca
    14641 cccacgccga tgatatccgt cagcgctgcg gcgccctgct ggaacacacc ggtctttatg
    14701 aacgcatgac cgccgaggat aacctggaat tttatggccg tgtctggcat ttgaacgccg
    14761 ccgagcgcca gcagcgcatc cacgacctgc tcgacgccgt gggcctctgg gatcgccgca
    14821 aagaacccgt gggcaactgg agccgcggca tgaagcaaaa gctggctgtg gcccgcgcca
    14881 tgcttcaccg ccctcaaatc atcttcctcg atgagcccac cgccggcctc gatccgattg
    14941 ctgccgtagc cctgcgggat gaactggctg ctctggtgca gcagcagggt gtgactgttt
    15001 tcttgaccac ccacaacctc gccgaagccg aaaagctctg ccagcaggtg gcggttatcc
    15061 gtcagggtca gcttatgatg atcggctcgc cggatgaact gcgccttcgc cagggcggtt
    15121 cccgcctgga gatagtgggg cgcggcttta ctgaggcggc cctgtccatt ttgcgacagc
    15181 agccccaggt caaagaggta acccgtcagg atcaacacat caccatcgcg ctcaaggacg
    15241 atgccgatac agcccccctc gtccagcttc tggtcagcca gggcgtgcag attgacgaag
    15301 tccgccgcgg caaagcctcg ttggaagagg tctttttaac cctcatggag gaagacaagc
    15361 atgttgaatg atatttttac cctcatctgg aaggaatgga aagaggtttt cctccagcgc
    15421 agttccggcc gcatgggcct ggtcagtctg ttgatttttg tgggcatgtt cggcatcttt
    15481 ttgcctttgc agtccggtgt cgagtggctg gtaagcccca tgagcatggc gctggcaatg
    15541 tggatgccca tcttcctggt tctgggcctg gtcaccgatg cttttgccgg tgagcgcgag
    15601 cgccacaccc tcgaaacact gcttgccagt cgcctctccg ataccgccat cctgttcggt
    15661 aagattggcg ccggggttgc ctatggtttt acctttgccc tggtgacgct gattgttggc
    15721 gctgtcacca ttaacatcgc tttcccggcg ggcggcctca agttttacac ccccgcgctg
    15781 ttctttggcg ggctggcctt cagcctgctc atctgccttc tcatggcttc gcttggtgtt
    15841 ctggtctcgc tccggtcacc caccgcccgg gtagcctacc agaagctttc gattgtcatg
    15901 ctggtgattg tgctgctgcc aacgcttttg ctgccgctgc tgcctgaaga tcttcaaatg
    15961 tcggtgcaga tggctctgca aaacatcaac tggggtgcca tcatgctggt agcggctgtg
    16021 gtattaattg ccgccgttgc cgtgctgatt ggccttgcca aagcccgctt ccagcgcgcc
    16081 aaattgatcc tggactagcc gcaggtgaca agcctcatcg ttacaggtga ttttttgctc
    16141 acacctatcg cctccggtat gtgatatgct taacctgtct gatctgaccc aaaaaggaga
    16201 cataaaatga ccaagacttt ggataatcta caagccgctt tcgctggaga atcccaggct
    16261 aaccgccgct atctggcctt tgccaaaaag gccgacgaag aaggcttcca tcaaattgcc
    16321 cgcctcttcc gcgccgcggc ccatgctgaa accatccacg cccatgccca tctgcgtgcc
    16381 atggatggcg tcaaatctac cgccgagaat ttgcaggaag ccattggcgg cgaaagctac
    16441 gaagtaacca ccatgtaccc gcccatgatt gaagaagcca aagccgaagg cgccaccaag
    16501 gctgccaaga gcttcagcat ggcctgggaa gtcgaaaaag tccacgaagc gctctaccgc
    16561 catgcactcg aaagcatcgt tcaggacgaa gaagaatacg actactacgt ttgcccgatt
    16621 tgcggttaca cccacgcccg caccgccccc aacaaatgcc ctgtctgtgg cgctcctggc
    16681 tcaaaattcg aagtcatcaa ataaccctaa cgctcttcat ggttctaaaa tcccccggca
    16741 tcccggggga ttttgcttgg tttattcagg tgatatttgc ccggcttctc tgtataataa
    16801 gggtaatcac agacaattat tgtgtggctt taatcgcgtc ctgtgatata ctaaacccgc
    16861 tattccgata aatttagtaa gctttcgccg aggtggtcat gctgcaagat tactaccctc
    16921 tcattatcat cttcactctg gctctggcgc ttggctttct ggtggttgcc ctcgggacga
    16981 tcttcggtcc ccgccgccct accaaacgca agggcgcgcc ctacgaatcg ggcatggtcc
    17041 cctatggtca gggcaagcgc cgcatgtcag tccgctacta tctgattgcg gtgcttttca
    17101 tcctctttga tgttgaaatc gtcttcttcc tgccctgggc ggtcatcttg cgtgaactgg
    17161 gcatccccgg actgattgag atgattctct tcctgttggt attaatggtg ggcttcatct
    17221 atgcctggaa gaaaggagcg ctggaatggg attagagcaa aaaaccggca acctgggcgt
    17281 tgtcaccgtt aaactggaag acgccatcaa ctggtcgcgc accaattcaa tgtggcccat
    17341 gctctttggt ctggcgtgct gcgccattga gatgatggct gcccaggctg ccaattacga
    17401 tatgagccgc tttggcatgg aactgatgcg cgccagcccg cgccagtcgg atctgatgat
    17461 tgttgccggg cgcgtcagcc gcaagatggc ccccgtgctg cggcagcttt acgatcaaat
    17521 gcccgacccc aagtgggtaa ttgccatggg cgactgcgct tcctgcggcg gcgtgttcaa
    17581 caactacgcc attgtccagg gagtggatga ggttgtcccg gtggatatct atgtggcggg
    17641 ttgtccaccc cgccccgaag ggttgattaa cggggttatg gcgctgcatg aaaaagtcaa
    17701 gcgagagaaa ctgaaggatt gggcctgaaa tggatgcaca caatcaaccg atagctgctc
    17761 tttttgtcga tcggtttggc gcccaggcag ctgagtttcg cggcgaactg accctcacac
    17821 tgcctgctga ccaggcccct gccgcggccc tggctgcccg ggatgaactg ggctacaacc
    17881 tgctccttac cgccaccgcc gtggactact ggccccagca gtccccgcgt ttccatgtga
    17941 tttatcagct ccgcagcctc aaaaccaatt acgttttgcg cctgtgcatc ccgctcaacg
    18001 gcaatgcgcc cgtcatgccc acgctcgaag gcatctatcc caacgctaac tggaaagaac
    18061 gcgaaatctg ggatatgttt ggcatccgct ttgagggcca ctctgacctg cgccgcatca
    18121 tcctgcccgc cgactgggaa ggtcaccccc tgcgcaaaga ctatccgctg ggttatgaag
    18181 agccgcagtt caccttcaac tttgacgaaa ttcgcctgca caagccccgc ggcgaggagt
    18241 gaacccatga gtgatattca agaagtcaat ctaggcgaac tgacccatct ggtctcccct
    18301 cgcgccctca ccggcgaaac catgctgttg aacatgggcc cccagcaccc cgccacacat
    18361 ggcgtgctgc gcctgctgct cgaactggat ggcgaaatca tcatcacctg catccccgat
    18421 attggctttc tgcacaccgg cgttgagaaa aacatggagg ccaaaaccta ccagaaggcc
    18481 gaggtgatgt ctgatcggtt ggactacctc aacccgattg gcaacaatct ggtttactgc
    18541 ctggccgtgg agaaactcgc cgagctcgat gtgccgcccc gcgcccaggc tctgcgtgtg
    18601 atcctggctg aattgcagcg tatctcctcg catttactct ggctgggcac ctccgccctc
    18661 gacctggcag ccatgtctgt cttcctctac ggcatgcgcg agcgcgagat catcctggat
    18721 atttttgagc tggtctctgg cgcccgtatg atgaccacct atgttcgccc cggcggcctc
    18781 tggcgtgatg tgcccgtaga atttgaaaaa gccgtgcgcg attttctcgc cctgatgccc
    18841 cgccgcctgg ctgaatatga tgccctgctg accaaaaaca ccatcttcct tgagcgcacc
    18901 cgcaacattg gcgtcatctc tgccgaagac gccgtccgct atggcctctc gggtccggtg
    18961 ctgcgcggtt gcggcgtcgc ccacgacctg cgcaaagatg ccccctacag cggctacgag
    19021 cagtatgatt tcaacattcc cacccagacc gccggcgata tttacgcccg ctacctcgtc
    19081 cgggtgcagg aaatgcacga gtccctcaaa attgtccgtc aggcgctgga taatctgcca
    19141 tatggcccgg tgcggtccaa caaccgcaaa tttgtccccc cgcctcgttc tgagcttggc
    19201 accagcatgg aagccgtcat ccaccacttc aagctctgga cggaaggctt ctcggttcca
    19261 cagggggcgg tttatgttgc cacagaatcc ccgcgcggtg agttgggcgt ctatctcgaa
    19321 agcgatggcg gtcccaaacc ctaccgtatt cactttaaca cccccacctt tgccaatttg
    19381 caggccctgc cgctgatggt gcgcaaggcc tttgtcgccg acgtggtcgg tgtgattggc
    19441 agcattgaca tcgttctggg agatgcagac cgatgaacct tttacttcaa caatcatccg
    19501 ccgaagttca acagatcctg gcgcactacc ctgccgaaca ccgccgtgcc gccgttatgc
    19561 cgctgctctt tttggcacag cgcaccaccg gccgcgtgga tagtcaggcc ctggccgata
    19621 ttgccgagat caccggcatt tcctccacag acgtggcctc aattatgggc ttttacaccc
    19681 tcttccacgc cgaggaagac ggcggcgggc gcttccgggt tcaggtctgc accgatatgc
    19741 cctgcgccct gcgcggttcc cgccagtttc tcgctgcctt ttgcgaagcc ctgggcatcc
    19801 agcccggtca aaccaccccc gatggcctga ttaccgtgga agaggtcaaa tgcctggctg
    19861 cctgcgacca cgccccgttc ctacaggttc agggcgatgg cgaactgacc gattacccgg
    19921 atcagaccgt tgagtctgct ctggcacttg ttgaaaccct gcgccagaaa ccctgggcgc
    19981 gctactaaag gggcgcatta tgacagaata cctgctctta cgtcatcgag atctcccgga
    20041 tattgatcag ttgcccatct accgcgccaa cggcggtttt gccgcctttg agaaggccct
    20101 caagacccag cagccggtag acgttttgaa taccgtcaag gcctctggct tacgcggccg
    20161 cggcggagcg ggcttcccca ccggcaccaa gtggagcttc ctgcccaata ccatttggcc
    20221 tcactatgtt gttgccaatg cggatgaatc cgagccgggc accttcaaag accgtgaaat
    20281 cctggaacgc aaccccttcc agttcctcga agggttgatg ctggcctgtt ttgccgtcca
    20341 ggctcaggtg ggctatatct acctgcgcgg cgaattttgg ccgctggcac aaaaactcaa
    20401 ccagcgcatt gccgagctgg aacagactgg cctgctgggc gatcacattc tcggcacaga
    20461 ttacaccctc cgcctctaca cccacctcgg cgctggcgct tacatctgcg gcgaagaaac
    20521 cgccctgctg gaatcgattg aaggcaagct gggccagccg cggctgcgcc cacccttccc
    20581 ggcggtctcg ggcctgtttg gcaaaccaac cgttatcaac aatgtcgaga ccctgaccaa
    20641 cgtgcccctg attgtagatc gcggcgccga ttggttcaag accctgggca ccgaagacag
    20701 ccccggcgtc aagattttct cgctctctgg ccgcgtcaat cgccccggca actatgagct
    20761 gccgtttggc gtcaccttcc gcaccctcat tgaccagtac ggcggcggca ttttagatgg
    20821 caaagccatc aaggccatca tgccggcggg cgcatcttcc accatcctga tcgctgatga
    20881 ccgcaccctg gatactccca tggattacaa atcagtggcc gctattggcg ggcagttggg
    20941 ttcggcttct gtgattattc tggatgaaac cgtcaatatg gccgacctgg tgaaccagac
    21001 tgtgcatttc ttcaagcatg aatcctgcgg caaatgcacg ccctgccgcg aaggcaccta
    21061 ttggatggcg cacatcaccg accggctggc tgccgggcag ccttctcccc aggatgccgc
    21121 gctgctgggt tcggtcgccc gccagatgca gaacaagtgc ctgtgcgccc tgggcgaatt
    21181 ctccaccatg gccgtcgtca gcgccctgga aaaattcccc caggattttg ggatctcata
    21241 gaggagcagc ccagatgacc aaacagatta ccctgaccat tgacaatcag gtcattacgg
    21301 cccccgaagg catgttaatt gtcgatgccg ccaaacaggc tggtgtgaat atccccgtct
    21361 tctgctatca ccccaagatg gaaccggtgg gcatgtgccg tatgtgtctt gtggacgtgg
    21421 gccgccccat ggtagaccgc gccaccggac agaccgtgct ggatgccgac ggccgcccca
    21481 agattggttt tggccccaag ctggaaacgg cctgcaccac ccccgtttct gagggcatgg
    21541 ttgtgatcac tgagagtgaa aaggtcaaag ccgcccgcaa agacgttttg gaatttttgc
    21601 tcacctcgca cccgctggac tgccccgtct gcgacaaagg cggcgaatgc ccgttgcaaa
    21661 acctcaccct ggcacatggc cccggcgaga gccgcttcct ttactcagac aagcagcacc
    21721 tcaacaagcg tgtacccctt ggcgaactga tctttctgga tcgcgagcgc tgcattcagt
    21781 gtggtcgctg cacccgcttc cagcacgacg ttgtagatga ccccgtgatt ggtttctacc
    21841 agcgcggtcg ggcgctcgaa attgtaaccc tctccgagcc cggttttgac tcgattttct
    21901 ccggcaacac caccgatatt tgcccggttg gtgccctcac cactgctgac ttccgttttg
    21961 gcgcccgccc ctgggagatg aagcccgcag cttccatctg caatcactgc cccgtaggct
    22021 gcaacctgac cttcaacgtc cgccgggagg ccgccgccaa cggccgcact gtgattaaac
    22081 gggtcctgcc gcgccagaat gaacaggtca acgaaatttg gatctgcgac aagggccgct
    22141 ttgtctacca gtacaccgaa agccccgatc gcctgactca gccgctcatc cgcaagaatg
    22201 gtgagctggt ccccgcctcc tgggacgaag ctctggagct tgccgcccag aaattaaagg
    22261 gtttgcagcc cttgctgctg gctggagggc gcctgtctaa cgaggcgctg ttcaacttgc
    22321 gacaggtcac tgaatcactc ggcgggcagt cctgtctcta tactcacatg tccggcggta
    22381 atcttgccgt tcaggctggc ctgacggcag gcagtaacct cggcggactg ggcaagggcg
    22441 atgccattct ggtggtcgcc agtgacttgc acgcagaagc gccgctctgg tggctgcgcg
    22501 tcaaacaggc tgctgcccgc ggcgccgcgt tgattgtgct cactgcccgc cccacccggc
    22561 tggacaagtt cgcccggttt gcgctgcgtt atgcccctgg cggcgaaggc gccgccctca
    22621 ccgctctgct ctcgggcgaa ggcgaagctg ctgcagcttt tgcaggtgcg cagaatgcca
    22681 tcatcttcta cggctcagat ggccttgatt tggtcggcac agaacgggtt gcccgtctct
    22741 ctgctgaatt actggaaaag accgggcacc tgggtcgccc aaataacgga cttgtgccag
    22801 tctggccgcg ggccaatgac cagggcgcct ttgaactggg gtatgccccc gccacagatc
    22861 tgcccgctgc actccgttct gctgctgtgg cctggattgc cgccgccgac cccgccggtg
    22921 atgcccccgc tcttgccgaa gccctggatg cgactgactt tgtcgtggtt caggagcttt
    22981 tcctcaccga gaccgcccgc cgcgccgaca ttgtcttccc ggcgcaggcc ttcaccgagc
    23041 aggaaggcag cttcacttcc ggcgagcgcc gtgttcaacg cttctacccg gctgtgcccc
    23101 cacagcctga tacccgcccg gattttgcca tcgccgcgca ggtgggtcag cgccttggcc
    23161 tgaaactggc agacagtgct gtctctttgg tctttgacca ggtggcgcag gttgtcccag
    23221 cctttaatgg actcaccttc ccggctctgg ccgaaacggc gccccagtgg cccgaaattg
    23281 cccgcagtga tctgtattac ggcggcacca gctatgacaa tcaacagggt ctgggcgtcc
    23341 atctggctgc gcccaaagcc ccgctccact tgcccgaatt gccccccgcc gtgccgcttc
    23401 ccgccgggct ggttgccctg ccggtcacac ggctctatga ccagggcgca ctgattgccc
    23461 gcaccgagct cctgcgcggc cgcttgtctc ccgccgcggt ttggatgcac cccgccacgg
    23521 ctgagcagca cggcttttca gaagggcagc cggttgaggt gctgctggct ggctgcaagg
    23581 tctctcgcac cgtctgtttg gatgataccc tgccgcaagg cttactgatt gcgctgcgtt
    23641 gatcgaggtg aacatggaat ctggcatgct ctgggaatgg attattaaag cggcggtctt
    23701 gctgctcatt atgacgggtg gctttgccta tgtcacctgg tatgagcgta aggtactggc
    23761 gcacatgcag gtgcgcatcg gccccaaccg cgccggcccg ctgggcctgc tccagccggt
    23821 ggctgatggc atcaagctga ttttcaaaga ggaactcatc cccgccgggg cagataaact
    23881 gctctttatc ctggccccgg ttatcaccgt catcccggct ttgattgtta ccgccgtcat
    23941 cccttggggt gaaacggtta acctgtttgg tcgcccggtg gccctgcaca ttgccgatgt
    24001 gaacgtcgcc acgctttaca tcatggcggt cacgtccatt tcggtgtatg gcatcacgct
    24061 ggcgggctgg tcgtccaaca acaaatacgc caccctgggc ggcttgcgcg ccaccgccca
    24121 gatgattagc tacgaactgg cgctggggct ttcgtttgtc ggccccatca ttattgctgg
    24181 ctccatgagc atgctggaca tcgtccgcac ccagcagaac acagtctggt ttgtcgtcct
    24241 tcagccgctg gctttcatca tctacttcat cgcctccatc gctgagatta accgctcacc
    24301 gtttgatatg cccgaggcgg aacaggaact gaccgccggt taccacaccg aatattccgg
    24361 catgaagttt gccctcttct tcatggctga atatatcaaa atgatcgcta tctcaatgat
    24421 tggcgcctcg ctcttcctgg gcggttttga cggccccttt gtggatcgcc tgcctggtct
    24481 gggcccgctc tggcttttca ttaaaatagt tctgtggttg tttatgctca tctggattcg
    24541 cgccaccctg ccgcgcattc gctatgaccg tctgatggcc ttcggctgga aaattctctt
    24601 ccccctggcc ctgcttaacg ttttgattac ctcgatcgtt gttgtcttga tcggataagg
    24661 agcaagcgat gctgaaagga ttgctcacca cactcaaatc cctgctggag aagcccgtca
    24721 cggtgcagta tcccaaagag aagcgtcccg tccggccgcg cttcaaaggc cgccacgtcc
    24781 ttaagcggta tgataacggc ctggagaaat gcattggctg cgcgctctgt gctgctgcct
    24841 gccctgccga tgcaatcttc gtggaggccg ccgaaaacac cgatgccgag cgctattcgc
    24901 ccggcgagcg ctactcccgc gtgtacgaaa tcaacctgct gcgctgcatt tactgcggct
    24961 tctgcgaaga tgcctgcccc acagaggcca ttgtcctcgg gcacgattat gagctggcct
    25021 ttgcagaccg gcgggatgca gtctatacca aagacatgct gctggagccc gctccgcagg
    25081 gcgcccccgg caccccgcag cgcacaccgc ccggcgtctt cctgcgcccc attcccgata
    25141 tgaaagatcc agagtaggag cccgcctatg cctgtcattc aaattgtcat ttttgccatt
    25201 cttggattgg tggcggttgg caccgccgtg ggcatgttgc tcagccgcaa tgcggtttat
    25261 gcggcccttt tcctggtgct caactttgcc accatcgcca tcctctacct gatcctgggt
    25321 gcgcccttta tcgccctggc gcaaatcact gtctatgcgg gcgccatcat ggtactgttc
    25381 ctgtttgtca tcatgctgct gggcgttgaa aaactgccct tcaaagagcg tttgcgcggc
    25441 cagcggctga tcgccatcct gctgggggtg gtcttcctgg gcgaggcagt gctttacctg
    25501 gtctatcgcg gtcatgccgg tcagttgccc ctccccgatg ccatccagtt cgtctctccg
    25561 gcagagttgg ggcgggtgct tttcagccag tacatgctgc cctttgaaat cacctcgatt
    25621 atccttctgg ctgcgctggt cggcgccatt gtcttgaccc accacgataa gcccacccca
    25681 gcggcagaac agatctgagg aggtcgccat gccaatcacc tactacattg tgctctcgtc
    25741 catccttttc gtcctcggcg taatcggggt ggtggtccgc cgcaatgcca tcgtcatctt
    25801 catgtcgctg gagctgatgt tcaacgcggc gaacctgtta tttgtggcct tcgctgcgta
    25861 ttaccagatc gtcaccgggc agctcttcgt cttttttgtg atgaccgtgg ctgcggcaga
    25921 agttgcggtt ggcctggctc tgatggttgc tatcttccgg gccaaacaca gtattgatgt
    25981 tgatcaaatg agcagcctga aagggtaaac catggataca ctttttgcac tggtcccctg
    26041 ggttgtcttc gcccccgtgc tgggcattct gatcaacttg ctggtcggca agcggctggg
    26101 cgagaaggcc gtcggcatca tcggcagcct ggcttctggc gcggccttta tcatctcggt
    26161 gatcctggcg gtggcgctgg gccagcaccc tgagggcgtg gttcaccccc tcgccgaatg
    26221 gatgaccatt ggcgatttct ccgtccgctg ggccttccag gtggataccc tttccaccac
    26281 catgatgctg gtggtcagcg gtgtgggcac cctgatccac atctactcca tcggctatat
    26341 gcatgccgat acccaccatc acggcgaccc ggggcgcttc cgccgcttct ttgtctatct
    26401 caacctcttc atcgctatga tgatggttct ggtgaccgcc gacagctacc tgatgctctt
    26461 cgtcggctgg gaaggtgtgg gtctgtgttc ttacctcctg atcggtttct ggtacgaaaa
    26521 aggcgaagcc ggcctgggca atgcccgcgc cgggcgcaag gcctttatcg tcaaccgcgt
    26581 gggtgacttt ggcctgttga ttgccatgtt cgtcatgttc tgggcctttg gctcgctcga
    26641 ttttgccacc gtttttgccc aggctggcac ggtcgccgct ggcgcattgc tggctatcac
    26701 tttctttatg ctcctggcag tgacgggtaa atcggcgcag ctcccgctct ttgtctggct
    26761 gcccgatgcc atggccggcc ccaccccggt ttcggccctc atccatgccg ccaccatggt
    26821 tactgcaggt gtctatctgg tagctcgctc gcagcccctc tttgccctcg tcccggcggc
    26881 tcaaaccgct gtcacctggg tcggcgcact gacggccctc ttcgccgcca caattgcggt
    26941 tggacagtat gatatcaaga aagttctggc ctactccacc atcagccaat tgggcttcat
    27001 ggtcgctgca gttggcatgg gcgctgtggt cggcggcatg ttccatctga ttacacatgc
    27061 cttttttaag gcgctgctct ttctggcggc tggctcggtc attcttggcc tggagcgcgg
    27121 ccacgaagcc gccctcaacg gtcaacacct gcgccacgat cactttgacc cgcaagacat
    27181 gcgcaatatg ggcggtttgc gcccccgcat gcgcatcacc tactgggttt atctgattgg
    27241 cgctctagcg ctggcgggca tcgccccact ggcgggtttc ttctccaaag atgaaatcct
    27301 ggctgcagcc agcgagcagt cactggctgt gtttatcatt ctggccattg ccgctttctt
    27361 tacggcgttc tacatgggcc gccagttgat cctggtcttt ggcggcaagc ctcgctcccg
    27421 cgccgccgaa ggtgcggccg aaaattcacc catcatcact gtcccactga ttattcttgc
    27481 cgttctggcg gctctgggcg gtctgctcaa cctgccgggt ctgcactggc tggagacctg
    27541 gctggaacat tccctggcgg gcctttccgc agggcatttc atcctctggg tggcgctgct
    27601 ctcgctggct gtggccctgc tggggttctg gcttgcctgg agcatctatg cccgcaaacc
    27661 ccgccagaaa cccgctgagc ccgacccact tgccgccccc ctgggtgata tctaccgggg
    27721 catggagcgc aaatggtggg ttgatgaatt ctaccgggct gtgctgctgc gcccggcggc
    27781 ctggctggca aacctgcttg ctggtccggt ggatcagggc ggcattgatg gcgccgtcat
    27841 gggcaccggg cgactgacgc gtctggctgc ccgcggcctc agcctgctgc aaaacggctt
    27901 tgtccgcggc tacgccctgg tgatgctgct gggcgttgtc gccattctca cttacctggt
    27961 gttgcggtaa tgcgcaggcc gccactgaat gaagaggaac acttatgaac tggtggacta
    28021 accccctcgt catactcatc gccttcccgc tggctggcgc tgcgctgatt gcgctcatcg
    28081 gcagccggca gactcgcctc atccgctggc tggcgctggc cgtcacgttg attgaattcg
    28141 gcctctccct ctggctgctg gcccgcttca accccgccga tggcaatctg cagctcatgg
    28201 tggatcaacc ctgggtggac ttgtttggcc tgccggtgcg cctgcttctg ggcgtggatg
    28261 gcctcagcct gctcatggtg ctgctgacca ccctcctgac cccgattgcc atcctctcca
    28321 cctggactgc ggtggaggat cgcgtcaagg gcttcatgct tttcttcctc ctgctcgaaa
    28381 gcgccatgct gggtgttttc ctcgccctcg atctggtgct cttcttcctc ttctgggagc
    28441 tgaccctgat tccaatgtat ttcctgattg gcatctgggg cggtgcccgc cggattatgg
    28501 ctgcggtcaa gttcttcctg ttcaccatgg caggctccat tttgatgctt gtcgccattc
    28561 tttacatgac caacgcggct ggcaccgcgg ccctgcccga actgatgcag cagcgggcgc
    28621 tcttctctgg cgcagcgtta tggctctttc tggcgtttgg catcgccttt gccatcaagg
    28681 tgcccatgtg gccgctgcac acctggctgc ccgatgcgca tgtcgaagcc cccacagccg
    28741 gctcggtcat tctggcggct gtcttgctca agatgggcgc ctacggcttc ctgcgcttca
    28801 atctctcgct ctttccgcag accttcgtcc aattggctcc ctggattggt ggtctggcgg
    28861 tggtgggcat cctatatggc gcagccgtgg cctatgccca aaaagatctg aaaaagctgg
    28921 tggcttattc ttctgtcagt catctgggct ttgtgatgct cggcatcgcc gctctcaatc
    28981 cccagggcct cgctggcgca gttctgcaaa tggtcaatca tggcgtcagt accggcgccc
    29041 tgttcctctt tgtcggcatg ctctacgagc gccgccacac ccgcgatctg gctgactttg
    29101 gcggtctttg gaaggtgatg cctatcctgg gcgccctaac cctgatcatt atgctttcat
    29161 cggtgggcct gccgggtctc aacggctttg tcggcgagtt caacatcctg ctcggtgctt
    29221 ttggctccag tgcgctcggc agcttctggt ttgctgcgct ggccgcgctg ggtgtggtgc
    29281 tggcggcagc ctatcttctc aaaatgtttg agcagacctt cctcggcccc atcaaacacg
    29341 aagaaaatcg agccctgctc gacttgaacc cgcgggagat catcaccctg ctgcccctgc
    29401 tggtgttgat gttctggata ggcctctatc cggcaccctt cctcaacttg ctgaacccca
    29461 cggtgacgca aatcctcagc ctggtggcag ttccttagtg gaggttgacc atgatccaaa
    29521 ctgacctgta tgtcctcctc cccgtcatta tcgtcatcgc ctgggcgttg atgctgctcc
    29581 tggtggacct ttgggttccc aaaaaccgca aaggcatcac cgcgggtctg gctgcctttg
    29641 gcttggccat tgccctgggc ttttcgctct cgcagtctgg ttcaaccttg cagggcttca
    29701 gcggcatggt ggtcctggat ccctttgccc gcttcctcaa cgttttgatt ctgggcagtg
    29761 gtctggcagc catcgccctg gcttacgatt acctcaaacg tatgggcctg gagcgcggcg
    29821 agtattacac cctgctgctg atctccatca gcggcatgat gctcatggct caggcgcatg
    29881 atctgattat cgtcttcctg gccctggaac tgctctccat cccgctctat gtgctggcag
    29941 gctttgcccg ccctcgcctg gaatcagaag aggctgccct caagtacttc ctgttgggcg
    30001 cttttgcctc gggctttgta ctctatggtg tagcgctgat tttcggcgcc actggctcaa
    30061 ccaaccttga aagcatcatg ggctcaattg gttcggccaa ccccgccatg ctgctcatcg
    30121 gtgccgggct gctgctggtc ggctttggct tcaaggtcgc cgcggtgcct ttccacatgt
    30181 gggctcccga tgtctatcag ggcgcaccca cccctgtcag cggttttatg tcggttggcg
    30241 tcaaagctgc cggactggca gcgctgctgc gggtctttat tgtggccttg cccgcacttg
    30301 ccgccgatct gacacccctg ctctatgcgt tggccattct caccatgctg gtcggcaatc
    30361 tgctggcgct ggcccagacc aatctcaagc gcctgctggc ttattccagc attgccaatg
    30421 ctggctacct gttgatggcc tttgtgcctt atggcaataa ggccatggtc gccgaatcca
    30481 atgccgccat gctcttcttc ctggcggcgt atgcgctggg tagcctgggt gcctgggccg
    30541 tggtagtgag cctggagcgt gccgagggcc gcggcctgga attggacgat tacgccgggc
    30601 tgggacgcaa atatccctgg ctggcaatcg ccatgctaat cttcatgctc tcctttaccg
    30661 gcgcaccgct cacgctgggc ttctggggca agttctatct cttcaaggtt gccgtggatg
    30721 ctggcttctg gccgctggcg ctggttggct tgctcacctc ggttatctcg gcttactact
    30781 acctgcgggt ggttgtagtg atgtacatgc aggccgggca gcccgaggcc cagggtgacc
    30841 ggtgggtcac cctgctggcg gtggctctgg cagctttgct ggtgctgctc agctttttcc
    30901 caggtccctt gctcaacttt gcagccctgg caggtctcta atctgacaaa aaaccggctg
    30961 gattttcacc agccggtttt tttgtgcttt gtcgtcaggc ctatgccgca atctcggcca
    31021 cagcttcggt cgggaatgct ttttcttccc caactcggta gacctcaccc aggagtgcat
    31081 acgtgatcag gccaatgtag aaagtaaccg gtgcggttac aaatggaatc aggaagcaca
    31141 gcaccagggt catgtaaaga atctggaccc caatcatcag aagcgcatag acaccaatga
    31201 tgataatcag tgcgatcata aaaccgctga agttcgcccg gaaaatcttc caccatttgc
    31261 caatctggaa tgccgcgcca aactcgtttt ttgccgcagt atgcacctgc attggcggca
    31321 ggagaatgat ataaacgata taaacaacca tcattacagc aatcatcagg aacatgaaaa
    31381 gctgagctat caatactaaa acgattgcca ggcctgccat atcgctgttg ccctctgcca
    31441 ggaagggtac cgcagccaat ggggcaaaca ttaagaaata aagcacgctg acaatcagga
    31501 tcatgggcag ggaatagaca aaaattgcgc caaacaccct caacccgtcc atgaacagct
    31561 cgccccagtt atcccattcg ggcatggtgg cttcgccact gcctcgcgtc acgcggcgca
    31621 gcacctgaga caggtaaccc atgacaatga ttcctggcag gatggggatg ataaactgtg
    31681 cgaaaatcag cagcgatcca atcagaaact tctgccctgc tttaggatcg cgaaatggaa
    31741 agcccagtaa ggctttcagt ctctcagttg caaacaacga attcatatgt ccctcccgga
    31801 cagcaaaatg atagaacgaa ccatttgatt atagcaaaat tttgctccgc cgctgcagat
    31861 tttacatccc ccgacctttt ttacaccaat tttacaaccc gaggtaagac cgcccacgca
    31921 gagatcttgc ctcgggtttt gaatggctta tcgtcttccc agcattcagt gcttattcct
    31981 gacaccgctc accttcaatc aaccgcacgg cccggtcata gaataggtaa tcccaggccc
    32041 agttgatcag caccaccagc cggttgcgga agcccaccag ctgcatcaaa tgcaccacca
    32101 gccagatcat ccaggccata aacccgtgaa attccagctt gcccagccgg gccaccgcct
    32161 gattgcgccc aatcgtcgcc attgctccgg ggtcctggta gtggaagggc tgcaaggctt
    32221 cccccttcat ctgccgcacc aggttatctg cggcggtctc ggcttgctgc atggcaaccg
    32281 gcgccaccat cggcagtgga cggccatcct cacccgccag cagggcggca tcgccaatca
    32341 caaacacttc gggatgttcc ttcaattgca gggtaggcag cactgccaca cggcgttgag
    32401 cgccctgttc agcccccagc gtatccatga tgctcgcggc ctgcacaccc gcgccccaga
    32461 tcacagtatg ggtgggaatg atttcgccac ctttcagcgt cacacgcata ccatcaaaat
    32521 tctccaccgc cgccccaaag cgcacctcca cgtgtttctg ccacaatgaa cgggcggtct
    32581 cctcgcccag gctggcaggc aggtgggtca ccagacggtc agccgcttcc agcagcaaaa
    32641 tgcgcacctc tttcaagttg agcgccggat aatcctttga cagcaccagc cggatcagtt
    32701 ccgagatcgc ccccgcacat tccactccgg taggtccgcc gcccaccacc acaaacgtca
    32761 gcagcgcccg ccgggtatcc gggttgggtt cttgtgcggc gcgttcaaaa cagctcaaaa
    32821 cgtggttgcg gatattggtc gcatcggtca gagttttcaa tccaaagcca tgccgggcaa
    32881 tggcatccag cccaaagaag ttggtctgcc caccagcggc cagaatcaaa tagtcgtaag
    32941 gcatatcgcc ggtgtttgtt tcgagggttt tagctgccag gttgacgcgt tgcgcttcag
    33001 ccatgcggaa ttccagattc ttctggcggc gcaaaatagc ccgcaccggg taggcaattt
    33061 catccggcga gatgcccgcc gtagccacct gatacaacag cggctgaaac aggtgatagt
    33121 tattccggtc caccagcgta acccgcacgg gccgttttgc cagggcgcgg gcggcgcgca
    33181 gtcctccaaa tccagccccc aggattacaa catggggtaa gttctgatca atcatcggca
    33241 ttgtctcctt cattgcaatg tccccatttt actcaaatca tatccctcag gtcaaatttt
    33301 gtagaacctg tcccatactt ctcacccggg gtgggaaata tttccttgtg aaccctgtca
    33361 tcaaagagga taatgagact gcaacagtcc attatcaagg agaatgacca tggagaccaa
    33421 acccaccttc aaagttcgtt tctggggcgt gcgcggcagt taccccaccc ctggccccga
    33481 gaccgtccgc tatggcggaa ataccccctg cgttgagctg gaagtgaatg gccgcaccat
    33541 catccttgac agcggcaccg gcatcatccc gttggggcgc agcctggccc gccgggcgcg
    33601 tgaacgcggc ggctcgctgg aagccacgct tctgctcagc cacctgcatc atgaccatac
    33661 ccagggcttt ccgttctttg tgccggtcca tttccccaat gccaacctgc acctctatgg
    33721 ccccaatctg ctcggcgccg acccgcaaat gacccttgag tcggtcatgc agccgcccta
    33781 ttttcccctg cgcctggcag acctgaacgc ccgcatcact ttcggcaccc tgcgcgagac
    33841 tgacctgctg ctcattggcg aggcagtcgg cggcgttacg gttcgcccca ccagcgagac
    33901 tgcccagtat gaccatgccg atgtggttca ggtgcgctcg ctgcgttcct acgcccaccc
    33961 caacggcgtg atgcactaca aaatcagttg gaacggcctc tcggttgttt atgccaccga
    34021 cactgaaggc tacgtgaacg gcgatcagcg cctggcagcc tttgcccgcg gcgcatcctt
    34081 gctgatccac gatgcccagt acaccgatga gcattacctg gggctgctgc ccggcatttc
    34141 aatcacccag ggctatggtc attccaccgt cagcatggcc tgtcaggcgg ctgcctcggc
    34201 tggcgcccag cgcctggcgc tcttccatca cgctcccgaa tacgatgatg cccggctcga
    34261 ccaggtggcg gttcaggctc aggccctctt ccccgacgtg ctggtcgccg ccgaaggtct
    34321 tgaaattgac ctggccgccg gccttgacat gcaaacctca aaaataaatc tccccctggc
    34381 tgtaagctga atggaccttt cctcggctat aataggaggt gcggcatgtt aaccgctgtc
    34441 caatccccgt tttgcttatc cagaattccc atgtcccctg tctcgcctca aaccctggct
    34501 caaatcccgc tgctcaatgg cctcactccc gagcagtgtg attatctggc tgcccggctg
    34561 cacctgcgta actttccggg cggggtagat atcatcaccg ccggagccga aggtcaggtg
    34621 atctatatct tgcttcaggg cacggtcaag gtctatgtgc ccgagctgaa cggcaacgag
    34681 gtgattgtca atatcctcgg ccccggcgat acagtagggg agatgagcct ggtagatcgc
    34741 atcagccgct ctgccagcgt catcaccatg gagccaacca ccacactctg gatgggtcag
    34801 gccgatttcc aggctgccct cgagacgatc ccggtgctgt cgcagaacct gctgcgcatt
    34861 ctctcgcggc gggtccgctc atccacggcg cacattcgcg ctctggctgc gctggatgtc
    34921 catggtcggg ttgcccgcca gctgctgacc ttcgccgagc gctacgggca ggttaacgcc
    34981 gatggctcgg ctaccattcg cgtacgtctc acgcagggcg aaatttctga gctggttggc
    35041 gcgtctcgca agcgggtcaa tcaggtaatg gttgcgtttc gcaaggccgg ctgggtcact
    35101 agcgatgcca actaccacat caccttgctc aatcaacccg ctttgatccg cgtcagcgaa
    35161 accgccgcaa tcgagagcta cgaataacct acgccgggcc ctggttttca ggttggggga
    35221 gaggctcacc ttccggctgc tgggggtaat gcttgcttac ccagcgcgtt cccagccacc
    35281 ccaacacacc ccacaccgca atgatggctt ccaccgtcca gattttggtc agcgtatcca
    35341 gcccagccag ctgcgcataa gatgccagtg tatcaatcag cgtcttgtaa atgaaggcaa
    35401 tccataggcc gccgggcttg cgcgtggcaa tcgcatggaa catcaacaca ttggagaaga
    35461 tgtgcaccag caccacaaag aatcgctctg agaccggcgc caatccccac agcggattgg
    35521 cgttctgcgc cagagccgca atatttacgg tggacagcag ccccggcgat accatcgcca
    35581 tcagggttga cccggctgaa gccagcccca gcaccagcgc ctcaaaagcg ccaaacccaa
    35641 tcccaaaagc cagggctttc ttccagcttg ccttgcccac ccgcgtgtag cgcaggatca
    35701 accagatgaa caacacttca aaaataccgg tcagcagccc cacccacaga tgaaaggccc
    35761 attcaccaat cccaccgcgc gtttcgccga ttagcaaatt gtaaatcggt gtgttggctg
    35821 gaatggcaac cataaatttg agaaatacag agacgatcca tcccagcgct cccaggccca
    35881 ggtatcccca gcccaaccgt cgcactgccg cgtaaattac ataaccgagg gccaccagcg
    35941 tcatcccgat gccgcccagc agcgccagcg ggggcacttc gggtagccgc accgcctcgc
    36001 ccggaacgat gtacaggtcc agttgtgcgg ttaccggccc atcccagacc agccacaggg
    36061 catattcccc ggcaggcagc gtatccacca ccacgctgta cttgaacgtt ccgctgcaag
    36121 gctcgccttc ccagacagtt tgcccatcca ggtcacgtat aaccaaccga accgcattgc
    36181 cgttggtgct gccattgagc aaaatgccct gcggctcatt atcagttgct ggcagcaagt
    36241 gaaaacccgt ctcccattcc ccggcggcgg tttcggtcaa tgtgtgtccg gtcaaatagc
    36301 tgccccggtc aatatttggc gttccaacct gcggtgctgt agtgcatccc gtcagcagca
    36361 gtacaatcaa ggcgatgagg aggatccagc gtttcataat aacagctcca atgatgaaat
    36421 ctgcaaagca ttatactacg tccaaaattc ccctgagggc taaatcctcc gtttcatccg
    36481 cttgcccaaa tttgtattta tggtatagtt acattaaagt gttgtgatac gctggaggaa
    36541 tcatgaaaga acgaattttg gttgtagaag atgatgatgc cattgtccgg gtattgcagc
    36601 gtgccttgag ctatgaaggc taccaggtgg atgctgccgg agatggtgaa accggcctca
    36661 aaatcgcact ggaacaccgc cccgacctgg tgattttgga tttaatgctc cccggcatgg
    36721 atggtctgga agtctgccgc cgcttgcggg gggttggaaa tatgcccgtg ctgatgctca
    36781 ctgcgaaaga taccgtcccc gaccgcattc aaggcctcga cgctggcgca gatgactaca
    36841 tgatcaaacc cttcgatgtg gatgaactgc tggctcgcgt acgcgccttg ctgcgccgca
    36901 ccgaaaccga gcgcgccacc atcctcacat ttggtgatct ggtcttagat acctccaccc
    36961 gccaggccag ccgcctgggc cgggtgattg ccctcagccc caaagaatat gacctgttgg
    37021 agctcttcct gcgtcatccc cgccaggtgc tgacccgaga cctgatcttt gaccgcgtct
    37081 ggggctacga ctttggcggc gaaagcaacg tgctggatgt gtacattcgt tacctgcgcc
    37141 agaagctgga gcaggaagga gaaacgcggc tgctccacac cgttcgaggt gtgggttacg
    37201 tcatgcgcga gacggcctga tgtccctgcg tacccggctg acactgcgcc aggtggctat
    37261 atttgccctc gggctggttg cgctttgcgc actggtctat gccctggtca gctttgtcgt
    37321 tttgaataag atcgataccc tgttggcgga ttcggctgcc tcgctggtag atcgaatgcg
    37381 cctgaccgag ggcgataact ttgatattca atctttacgc accgttccat tgccagaaga
    37441 cctctatctg caggtctggg gtagtgaccg gctgttgcag tacagccatc cggccacatt
    37501 tcaaatcgcc atggataccg ccggctggga tctgggacgc cctgcttaca ccactgtaaa
    37561 ccgtaccggc ttgcaaatgc gcgtcttgtc cgtgcccctc gaaaccgccc gcggcccggc
    37621 cggcatgttg atggtgggct tcagcctctc cctgctgaca ctggcccgcc agacgctgct
    37681 gttgataata agtgtagtaa gcgtattgct cctctgtctc atggcgctgt cagcctatct
    37741 ttcaaacagt cagatgctca ctggcctgac caagctgacc cgtctgatgc ccgaattgat
    37801 caagatgaat gacctttcgc agcgcctgcc cgatacgctg ctcaaaaacg acgaggtcgg
    37861 cgcactcagc cgaaccttca atgccgccct ggatcgtctg gaaaccttgt tgacctctca
    37921 gcgccgcttc atggccgatg tcagccacga actgcgcacc ccgttgacgg tcatcaaagg
    37981 cgaggtgggt ttaatgcgcc gcatgggcaa gggcgataaa gaatccctgc acagcattga
    38041 ggccgaagtg gaccgactga cccgcatggt cggtgatttg ttgatgttgg ctcaggcaga
    38101 atcaggtcgg ctgcccctga acctgagcat gattaacctg gatgaagtcc tgatggaagt
    38161 ttaccagcag gctctggttc tatcaccgcc gggggttctg gtgcgcctgt tggaaatgga
    38221 gccgctggtg attgaggccg accgtgaccg catcaaacag gtgctgctca atcttgctgg
    38281 taacgccctg cgctataccg ccagtggcgg tcaggtcatg ttatcactgc gcagtgcctc
    38341 gggtggggtt caaatccagg ttagtgatac gggcaaaggc atccccaccg atgatttgcc
    38401 ccgcattttt gagcgcttct atcgcggcga gcgttcccgc aagcgcgaaa aagagggcgg
    38461 cctgggcctg gggctgtcta tctcctattt gattatcaaa gcccatgggg gcagaattga
    38521 tgtgacctca caggaaagcg tgggaaccac attcaccatc tggctgccgc tgacgcagcg
    38581 ctgatttgca gatggagcgc agacttttgc ccacatgttc cgtcctctgg taatttaacg
    38641 catcggcgcg ccggtgaggc tagccgacgc gccgtgcaga agggagagca ttggggctat
    38701 tagatcaacc gcgggctggg ggatcgccag cggaagttcg cgaaacaggc tgcccggaga
    38761 gggaatggta ggagggttct gggcgggaac tggtagccgt ggagagcggg cgggggtaag
    38821 gccgggcggc cggagccgga ttggaagctg tcgggcgggc tgccggggtg gaggtcagcg
    38881 ggcggccaac cggcactgag gctgaaggtg ctgtggtgcg cggacgagag tatccgctgg
    38941 acttgcgcat atccgagaag atgcgctcgc cggccgtgga aagtgtgccg atgaccagga
    39001 tacggatgat ccagaccagc actgccacaa aaatgggcac aatctgagtg atcacacggg
    39061 catcgaccac ggccaccgaa gccacggcat ggttggcaat cgccatcgaa acgccccacc
    39121 aggtaagcac ggcgttcatg gtggccgcca gcagccaggc gccgaagaga taccagacct
    39181 ctttgggttc atcagcgccc tgttcagggg tgaagaggcg ggcaatgccg gcgaaatcca
    39241 tgccgcagaa agcaatggcg aggatggtgg cccaggggat tccggcaaac tgaagattgc
    39301 cgaggagatc gcggagggcg taatcggtgg tggaatagtt gaagagctca aaggcgagga
    39361 gggccgagag gatcagcacg ccgaagagca agccggggcg aaagcggcgg aaagcgccag
    39421 tcaggaaatc agaagcagaa cgggtgggtg agaattccat ggaaaactcc ttatgagcta
    39481 gaacggttat tctagttcgt attatagaac atatattcta taatgtcaat aacgagtttt
    39541 ccgaaatttg cccgctttcg attagctgcc cagcttggcc agcttgaaaa acctccgcac
    39601 tacaagcgct aaaaataccg cccacggcac cagcgatgcc agtgagaaaa tcaacccaat
    39661 cgtccccgca gtagatggaa tggacatcaa aacgcccgcg gccagccccc agatggcggt
    39721 tgccctggag aacagctcac ttttcaacat cacagccgcc gtaatcaaca gcataattcc
    39781 attcaaaaca taatatacat caaaagccgt gcccttgtaa atcgccagca tagcctcccc
    39841 cgcacccagg aaaaccgcct tttgggtttc atcagcagct tccgcataga actggcttag
    39901 ccgcagcatc tcaaatgccg tattagaggc atagtaagcc gcaataccca ccaatcccag
    39961 caccagcgct atcagcatcg ctgatgggtg ggtgtgtttc aatgccataa aaagtgccag
    40021 gtaaatcaga attaaaaccg cattgttgat caggtaaagc agatccatgc ttaaaagccc
    40081 cagcagccag ctcttctgga ataatacaaa gaaatccggc acagtttccg gcggcggcca
    40141 gataataaag atcgtaatct gggcgaccat aatccccagc atagccagtc cagcgatacc
    40201 gccgaataga aagagtttgt tccactgggt ctcaatttta gcagttggcg tgtgggcagg
    40261 tttcatgtgt attttttacc ttatgtgggg gttatttgga aattcttgtc agacaactct
    40321 tttttaccat acctccagaa taaaagctac ccattcatcg ggaaatgaat gggtagcttt
    40381 cggtcactga gatcgaccgc aatagtctat ttatcgtgtt gatacagggc cttgattaac
    40441 agaatcccgc cgatccccat ggcaatcact gccagatcat atccgatcag catggcaatc
    40501 actgccacaa acggcaggcc ttcatttgct attcggcctg aagccagccc agttaattgg
    40561 gcggtcaata aaccgccgcc cagcaatgcc aacccaatcc ccagactcca gccaatccac
    40621 ttgagctgtg tccgggctcg aatagccgcc cagatcagca gcgccgcgcc gatgaagacc
    40681 acccacatta attcagccgg gataaggtaa tcgcacagga actcaccgga ttgaatactc
    40741 cctataacgc aggtcaccac catgaacaga attggaatcc acgccaacac cgtgccagca
    40801 atggcaagca ccttagtgaa tgtatttttc ttttccattt tatccttcct tgggaaacct
    40861 ttgacgataa tttattatag taggttaaac caattaatca cagccgtttt gggcgccatc
    40921 ttttttgcgg gcataaatca gcatcatagg cagcagaatt atactggaaa gccccagcgc
    40981 tcgccggaca ccaaaacggt caccgaaagc tcctaatcca gtgctgatac ccagtgtccc
    41041 gaccatacca gcctggctgc tcatcgaaat aagggttgct ctaatcttag aggggatatt
    41101 ttgattcatc cacgcctcag tgatgggagt actgaggttt tgtaacccac ccacaatcac
    41161 ccaggtggcc attgccaatc caaagtgctg ggtaagcgta aaaataacca ccgcaacggt
    41221 tgtcatagcc gaaagactta acaacgttcg cgccgcagcc ccacgatcac tcaatttgat
    41281 cttgcggcgg atgatttcag ctcccgcgag acagaacaat ccttgcagca tctccagcac
    41341 cccgaaccac aaattatctt tcagcgctcc cagcagtggc agcgtaatga ttggcagtgt
    41401 aaaactctgc aagacatggg cgcgcgaaaa tttatagaac ccatctgcaa atgcgctgcc
    41461 caataggaat gcccagaata aggcttgcaa ggtggtagag ccgcgcacca gtctgattcc
    41521 ttcagcaaat gttgccgaag atgctttcat ctgattccgc caagactggc ggttgatttc
    41581 agtgtttttc ttgaaatttg tctccggcat atagaggatc aagaaaatgg tcagcaagag
    41641 ccacagcaca ccccccagga taatcggggc ttggtaattc atccaggctg ctaaaccgat
    41701 gctcaagatt acgcccaccc agcctgccgg tgtggcaacc tgccgcccgc gtaaaaacac
    41761 attgccgaca ttgtcttcac ctacttcgtc ggtaatccag gcttgcaatg ctccgctgat
    41821 gcaggtatcg ccgattgctt caataaaacc agccagtaaa gcaatggcaa aaaacgggaa
    41881 tatacccacg gtcatgaatg ccagcccgac aaaaattccc ccaataatga ctgataatcg
    41941 ccgggaatac aaatcggcca cgatcccggt cggaatctcc aatataaaat ttgagaccgt
    42001 cagcacaatg tagagaactg aaacctcgat caggctcaat tgcatgaccg tgtagtagag
    42061 cacccaaatg ctggtgaaaa tcaacccgct gttgagttca cctgttgctg cgcgaatata
    42121 gtaaatcgat tcagccggca gcttcttcat cgattttttc gatcttaaag tacactgggc
    42181 gggttccatc tgtacagcat gcaatcaggc aattgccatc ctcataccac atgccaaaac
    42241 tacccccggc ggcatatccc accagtacct tgtaaatatc atcccaggcc caggggcaaa
    42301 aaccagccgg gatgcctcca aatacatcag tgataaattc ttgcccttct ctaaatactt
    42361 cgcagggccc cagcgtcttt ttacgctcat gttctacata gcgttccacc agatcctgat
    42421 taaatcctcg tttcaacaca gtgattttgc acttgaccat gagaatcttc ctgttaggta
    42481 attttgatga atggaaattc tggccgaaac accagcttgc agcgattatc tcacaaaaat
    42541 tggaacctca ctgtacagtt ccttgggcat gataaaataa aaccactttc aacaaccctc
    42601 aaggagacca ggatggacat tactgctctg cgcgctgaat ttccagtgac cgaccatgtg
    42661 atttacctca ataatgctgc cgaatccccc ctcaacaacc gggtccgtca gcgcctggca
    42721 gattatctca tccttgccag cgaagcaccg cataagaagc catcggtccg gcatgccgta
    42781 aaagccatgc tgtctgatct ctttggcggc gccgccgacg aatatgccct ggtcaccagc
    42841 acgggggtag gtgtcagcat tgtcgcggca ggctatccct ggaaacctgg cgataatgtg
    42901 gtcttacctg ccgatcagca ttggaataac actttcccct ggctggcgct gcgccagcgc
    42961 ggcgtggatg tccgcctggt tcccgtggat cccgattacc gggtcaatcc tcaagccatc
    43021 gccgaccggg tcgataaaaa cacccgcatc ctcgcaaccg ccgctgtgga attcaacact
    43081 ggcttccgtg ctgatctcaa gcagctcagc caaattgccc atcaagctgg tgcgcttttc
    43141 atggtggatg gcatccaggc tgcgggcgtc tgcccgttga acattgatgc tgatggcatt
    43201 gatattctgg ctgcggcagg tttcaaatgg ctctttggca tgcccggcac gggctttcta
    43261 tacgtcaacc ggcgggcgca ggatctcatc cagcccgtca tgcccggtat gtttgccgcc
    43321 gacaataatc acacccgcga cctgctctac ttccccgatg cccgccgcta cgagaccggc
    43381 acaatcgcct actcacactt ccattcctgg accgccggac tggaactgct caaagagttg
    43441 ggcattgtca atattcacgc ccgggtgctg cacctgactg attatctcat tgccgggtta
    43501 cgcgccagaa atataaaaat cacctcgccg gtagagcata tcgccgaacg ctctgccatc
    43561 ctctccctca caattggttc tccccaggag aatcaggcgc tctacgaaaa gctgttcgcc
    43621 caggatatca tcgtggcccc gcgtgatggc cgcatccgcg tcagcccgaa cttcttcaac
    43681 accgaagctg aaattgaccg actcttgcag gctttatagc ctggtaggtc attgattaat
    43741 gaggttggat aaccccaggt taatcaattt attggccgct gtatcgatcc caataaaaat
    43801 cacattggca atcagggcaa tgattaaaaa gataatcata atcggataca acagcaaagg
    43861 attctcgcca gttgccatgg gcgcacttgg gattacaaca aacaccgcca cgtaaatcag
    43921 cgtaatcagc acgctgctaa tcagaatacg cttgaggacg ttcggcagcc tccgccgcgg
    43981 tgcttgcacg ccttcgccct caacattgac ctccacgttc gctggctcac tgcctgctct
    44041 ggagcgtttg atccagcgct tcagccagtc ccacagcacc gaaacgccga acaaacccat
    44101 actcaatacc ccatataccc gcatgatgtc tttctctgtg atcgatccac ttccattggc
    44161 gtcaagcggc aggagaaacc caaaatattt taaaaccgac atgtcggttc ctacaacatt
    44221 aatgtatata aataccagcc ctgtaaaacc gatcaggacc accgcggtca aaaaactata
    44281 actgataaac ttcagaacat atcccaatcc aatcagcata atggctgtta cgcggctttg
    44341 attctgcaac atcgtctcct ccatggacct ttcttaatgg tgtgctggtt gtttctcttt
    44401 tatccacagc agcccaccca gtagaaaaat gaccagcccc agcccaaagt aaaacagctc
    44461 aatgggggtt gggccgggtg gaacctgcat tagaatcaag aattctccga caataaaccc
    44521 catcatactc aaccctgcca ccatcgccgc cactatccct gcattgagtt ttctgaaaat
    44581 tgtgatgcac gtcagcagcg cgctgccccc caccacccct gccagcagca gccccgggac
    44641 cgtgtaatcc ttgaaaatgc tgccctgcag ccattccagc ggaaaccgtt ccccctccat
    44701 gccggtcagc agtgccccac cgccgccaat cgcggtcagc gcaatgaacc ccgtcagtac
    44761 cccgatgagt atccgcagcc atttatattt catacttgtt ctctccctgc cgtatttcgc
    44821 cagcccctca gtgacgagtg ataaatggct caacgtcggc aataaacgtc aacgtttcgc
    44881 gctcaatctg cccggtcaac cgctccaggt cactgaaatc cgggttatcc accagaatgt
    44941 gctcaatgat ggaatcggcg ccctggatgg ttggcacaag ccgatattga gcccagccgc
    45001 tttcctgaaa cagcttccag tatttagcct gcacctgctt gttggcgccc gccagccaga
    45061 cctcaaaccg aaacgcctca tgcagaaaga caatcgcaat cttcaggccg cgctgcctga
    45121 acgattctgg attgagcgag aagtaagtca tatccatata gccggggtaa atgctgctgg
    45181 gcacggcaaa gtcggggtat ttgtttttga agtaggtccg caacgccagg atatagtcca
    45241 tcaatccctg ataagctgcc tgaaccgccc cctgctgcag cagctttttg tattcaatca
    45301 tatgctcctg aaaagttttc atggcgtctt atgactctgc tgaaaattag tttgattatt
    45361 ttgaaaactg cgaattacct gttgatatca gataagcgtt atttgtaatt gagcaaaatc
    45421 atcacaatta aaacacccaa caaaatcaga aatggcacag tgacaatcaa taaaaaacgg
    45481 acaagatttt ttcgcggagc cccattactg acgggctgcg tttccttgac catcatttgt
    45541 attgctgcca aatctccagc ggattgattt ttttctaata gccctaactc cttaatggct
    45601 ttctgtccct tccagagacc gaataatgcc agaacgtgga aaatagtaga cagccaatct
    45661 ccaaatgcta aagccaacag gccatctaat ccatataaaa tcatcccacc aattacaacc
    45721 cagcgcaatc tttttcttcc caagatacca catgtgaaaa aggtgcccgc taaaaggaaa
    45781 tctaatccat atccaatgac ccgaatgatc gtacctgttc cagagcttac cttgtttacc
    45841 acgccagaaa cgacgccatc aataaattga gttgcaccca agcctaccac aaaagtgatt
    45901 gacccacccg taaagaatat aatggagtta ataatggaca accccgcaat ccagaaaaac
    45961 caactaacgc cacctttcag ttggttctct aatgaaagca ttgtttttgc gattgcaatt
    46021 tgactcgtat tggcggcagt tgaggtctga tccatctttt ctccttgata agtgaatttc
    46081 tctggcttca gtgctccagg ataaatagcc gaaataatac tctgcttatt atacctgcac
    46141 aaataaaaaa agcccccgac ttaccccggc tgttcccaat ggggccagcc agagattgtc
    46201 ggaggcaaag attgaatctg taatcttatt gcaacagcga ccccatagcc ttgttgatga
    46261 agccggcggc ctgttttggg gccagccggg agagaaaatc catcattttg gcatcactgc
    46321 ccaccagaac ccgatagctg ttgcgctcaa tgccttttac aatcacctct gcggccacgt
    46381 tggccggggt ggtcttcatc gtcttttcag ggcgtgactt gcctgtatca atatgcgccc
    46441 ccgagttttg agcaatattg gtcgcaatcg ctcccgggaa gacgatggtc acatgcacat
    46501 tcgtccctgc catttctgaa ttcaaaccct cggtgaacag tttgaccgct gcctttgatg
    46561 ccccgtacag cagctgcccc ggcacgggca gaaagccccc catgctcgac acattggtaa
    46621 tattggcttc tggtcgggtc atcaaataag gcagaaaaac cttggtcata aagagcgtgc
    46681 cgtacaggtt cacgtcaatc acccggtcaa tttcggcata atccagttcc ttgaaattga
    46741 caaacttctg gataatcccg gcgcagttga tcaatccatc aaccgcctga tgcgccgcca
    46801 taaccgctgc cggcaaggcc tctaccgcag cccggtcggt gatgttcacc acatgggtcg
    46861 agagccgatc tttctggctg ccggccaggt tcaccgtctc ttgcagagcc ttctcgctga
    46921 tgtccacagc cgccacccgg gcgccttttt gtaagagcag catcaccagc tcccggccga
    46981 tgccattacc gccgccggtc acaacaaaca ccttcgattt cacgtccatt ttcttactcc
    47041 tgttggtagg ccaaaatcaa tcacttataa gataaataat atcctatata tttataatta
    47101 taatctaatt tattaaccat tacaatcact cgcacaggcc agttttagct tgtgcgagtg
    47161 atcattttga tgacgctgca tctctccccc gtcaggtgga tttgggcctc cccagtctac
    47221 ccgggcagcc cgctatcctg gcgggtgacc gccccctcca ataattgcgc tttcgttata
    47281 ttgcgaaagc tgtttcggta acccgcatag acagccagcc cataaaatac cagatctatc
    47341 accgaaaaag tttctttcag cagttcaaag gtcaatcgat agttaaatcc aagcagagta
    47401 tccaaaaagc tgagttccag acctttggct aaaaagccca tcgacgccaa aaaattaccc
    47461 agcagaacac tgcccagcgc aatcacgccg ccgataatcc cataaatttt gtccaaccct
    47521 ttacccaggg tcctgactcc aaacccaacc aaaaagccca caatgatggc aatccaaccg
    47581 atctgatact cggtgaagta ggtgatcgca gcccaggctg ccgcgccgat cagcccgcca
    47641 attgcgccgc cgatgatgcc gagcaccaga ttttgctcat gcttatagtt ttcaattttt
    47701 gcccgcacca tattttcatc aattcgatat tgcggtgcgg ccggcgctgg gggatttgtg
    47761 ttttccattt tttgtctcct taaaatgatt gaaatagggg aattttttgt gcagtgaaga
    47821 ctattgagaa atgatttcta agattatgcc gtcttccctg acttcaacga tgttaatgat
    47881 atacgcgtca atctcaagag ccattccgga gtgaacgcgt acagtttgat ctaaatcact
    47941 tttgtcctct acaaataacc acagggctgc agttaatcca ttcactgttt ctccgtcgcg
    48001 ggttgaataa tcttcagccc aaatattttc cacaccgatt tttagtgttt gaaatgtcaa
    48061 ttgcgtgttt gtttgaatgg taaactgatt ctcgttcaga tcttcaacct gcgcttcagt
    48121 tattgccggt gtggtgcagc cgccgagcac caaacaaata accgtaaata ttaataagcg
    48181 cgttaccctc tttattttca attcatctcc aggaggtgca aaatgggaag atcaataaac
    48241 tctctgtgtt tggatggttg gaaatgtccc tgctgtttag acaattatat cacttgtgca
    48301 gtctgcctca gccgggaatt gatttggagg cagggaaatt acgccagacg ttggtcaaat
    48361 atcccaaaag ttgaggactt gtgaaactgc accttcaaca gttgtttctg atgtgtttat
    48421 ttttagccac tgcaatttcg ttttttgcag caggctcaga agcaaagcct gctgtttttg
    48481 gtaataatct acaatctcgc tgttcgtacg accaaaattt tgcagatatt ttcgccaccc
    48541 ttcatttctc tcaaggataa tccttcgctc catatcggct tcgttaattt caagaacgca
    48601 taccttactg ttaatttctg ccaggcgttc atcaatagag cggacgagct cccaatctaa
    48661 accataatga aatacatgcg taaaatgaaa gcgctctaac aagaaaggaa tccgatgatt
    48721 cgtgcggttt cttgcattcc aatccatttg gcttgagcgc tcatgtatct tttcaaagaa
    48781 ctgaacataa aactctaata atgctatatg atcatcaggg acaagccctt ctaaggcttg
    48841 ttttctctct aaaactctct gcgtgtgatg ctccgaaatg accacagatg aaggataact
    48901 tttttgatta tagcggggac ttctggttaa agcctgaatc agggttgtct tcccggtaca
    48961 gcaaattcct tcaatgatta gaccagagac atcccagttc atatttgtgc ctttttgcta
    49021 gtgtctgcac cccgataaag cttttattcc cttgccccaa acgcctcatg gaacgccacg
    49081 ctgccttgcg gcatggggcc gtcaaatgca agcgcaaaaa aggcttctgg cggcacccct
    49141 tcaaggccca gaccggtaac attctcaaac ccaaaccgcc cataatattc cgggtgccct
    49201 accaggcagc agcctttggc tcccagaccc ttcaagcggg ccagtccttc ccaaatcagc
    49261 gctttgccaa tccccatccg ctgatctttt ggcaacacag ataccgggcc cagcccatac
    49321 caatccggcg tgccgtctga aatggcaata ggcgaaaacg caatgtgtcc caccaccgcc
    49381 ccatccactt ctgcaaccag cgatactgtc aaggctccgg ctgctcgcag cgccttgaca
    49441 ataaactgct cggtgtgatc gctgatctcc atcgtctcaa aggcggcagc cgttaccgca
    49501 gtgatgactt cagcatcgtc ctcacgctcg tctcggatca agatattctc gattggcatt
    49561 gtcatctccc gggtatgcaa aggattttgg ctggggtcag atgctcgttc agggcaactg
    49621 taccaattgt tgattatcca ggcgataggt gaaagttgcg ctttggctgg ggcagcacag
    49681 tccatcgttg ggcccatgca cgcgcaggtt gagcgtaatc aagccagctt gaatccctcc
    49741 cgattcaacc acaacccggt cgcccagaga cagcatggac acgttgttgg cggtcccatt
    49801 ctgattcaaa accgctgcca tttccacaaa atgcccggtg cctccgcttt gtgtggtcag
    49861 aaagacaatc gcgtcttcaa cgccatcccc gtcaatatcg ccgaacagca ccaggtccag
    49921 catatgggtg gtgtaagcct caggcgactc ctgggttgtt gggggtgtgc ggtaataaat
    49981 cccgtccgtc agttggaact gcccccagtc tgccgagtta tagaccccat tgcgcagctg
    50041 ctccagcgag agcgtgggcg cagccgttgc cggcgccgca gtctgtgttg cggtcggcac
    50101 cgccgcttcc ggcgtaacgg tggcaggtgg attgctctga gctatcgcag tcagtgttgc
    50161 gttcacgcgc tcatccaaat cctcggtggt tggcggcaac aacccgcacg ccacaatgat
    50221 aaacagcatc ggtatcagcc agagtttctt tttcatggct ctattgtagt cccatttgca
    50281 ggtaaaataa gactgtcttc atcatccctt ctcaggctgc cgcaggctgg ctcgctgccg
    50341 cggtcagcgt caggagcatc ccatgcccat tcccgaaagc tggcttcctg ccctcagcgg
    50401 cgaaaccacc caaccttact accagcagct ctaccgcttt gtcggcgaag aacgccgtaa
    50461 gtataaaatt tatccccccg gcgaagatgt cttcagcgcc ctgcgctaca cgccactgga
    50521 gcaggtgcgg gtcgtcatca tcgggcaaga cccctaccac gatgagaacc aggctcacgg
    50581 actggcattt tcggtgcgtc cgggcgtcac cccgccgccc tcgctgatca atatctacaa
    50641 agagctgcaa accgacctcc agtttcgcat tcctaatcac ggctgcctga ttccctgggc
    50701 tgagcagggt gtgctgctgt tgaacgccgt gctgaccgtg cgcgcccacc aggccaactc
    50761 acaccagggc aaaggctggg agagcttcac cgatgccgtg ctgcgcgccg tcaatgctaa
    50821 aaccagccgg gtggtctttc tgctgtgggg cagctacgcc cagcgcaaag ccgaatggat
    50881 tgacgccaac cgccacaccg tactgaaagc cccgcaccct tccccgctct cggcctcccg
    50941 tggcttcttt ggctgccgcc acttctccaa agccaatgcc gccctccagt ctgccgggtt
    51001 gcccctcatc aactggcagc tgccggatat gtaggtccgg gcatcttatg agatatttgt
    51061 gcttctaagt tttcggggcg attatccgtc tttcaattca tcccctgtgc tgagccactc
    51121 tccagccaga ctggagagcg gtattgcttt gatcttctca cccagtgagt aaggttttga
    51181 tcccggatag acgaccaaca ggtcagataa ttctaaatct tgcatggcag cccgcatgga
    51241 tggtgttagg cggggagcat caacgcgttt gcattctacc ccaattctgc ggccatcttt
    51301 aatcagcagt aaatccaatt cggctccgga gtgcgttccc caaaaatata cctcatctgg
    51361 agcagcggct ttaataactt cttcaatcac aaacccctcc catgatgcac cgctgcgcgg
    51421 atgtaaaagt aattcttgct cggtgtgaat acccagaaga atgtggagca agccagtatc
    51481 gcgaatatag accttgggtg attttacctg ccgttttccc aggttggcgt gccatggttg
    51541 aagcagccgt accatataaa gatcctggag caaatccaca taccggcgag ttgtgttctc
    51601 actcacattt agggtgcggg cagcttcagt cgcattccac acttgtccgt gataatgagc
    51661 cagcaggctc cagaatcgga aaaggctggt ggaaggaatg ctaaaaccga actgtggaat
    51721 atcgcgttcc aggaacgtgc gtataaaatc tttgcgccat tctaggctgg atgattcatc
    51781 aggcgcaaca aacgaaagtg gaaatccacc gcgccgccag agggtgcttt gtgcctgtac
    51841 gcccacttcc tgcaggctga atccgctcat ttgaagggtt gaaattctcc cggccaggct
    51901 ttcagaagac tggcgtaaga gttcgggaga ggcgctcccg aggattaaaa aacgaaccgg
    51961 tagaggtgta cggtctgcca gcacccgcaa cacaggaaaa agatccggac gccgttgaac
    52021 ttcgtcaatg accaccagcc ccgacagatt ctgcaaagcg gtcatgggtt gatcgagacg
    52081 ggccaggctt aatggatctt ccagatcaaa gtaattaaca gagtctgacg ggacgaattc
    52141 ccttgctaga gtggttttgc cacactgtct tgggccgacc agggctacaa cacgattggc
    52201 ttcaagtttc ttgtgaaggg tttgttggag gtaaacgcgg ttaatcatat ctgtataata
    52261 ccatgaaatt tcgttatcaa ataacgaaat ttcatggtat tttagggttt acggtcattc
    52321 ttttaactat ctatagagac tctgcaacca tagttgatgg gggatggccg gtactgagat
    52381 ccatcgattg gcagctgccg gatttgtaac ccgtatcgcg cgtcagacca ttcctgtctc
    52441 tacggtttac ctgcgcccca gccaccggtg aaccgaggcg gcatattgct ggtagtcctc
    52501 accaaatttc gccgccagat agcgctcctc cgggcggatc agaacctgaa tacagatcac
    52561 aattgaaagc accagcgccg ccaccgccca caaccggttg agcgccaacc ccagccccag
    52621 aaacagcatc acactcgcca ggtaaagcgg atttctcgaa taccgaaaca cccccgtatt
    52681 caccaaccgg ctggttggct gccccgggtc tgtgggctga ttgtatttcc gcagctcccg
    52741 gcgagccagc ctcacaacgc caatcgctgc aatgatcaaa acaaccccgc tgatgatgat
    52801 acccagccgc caaccccccg gcggcaccga aaggggcgcc accgcatgca cacccagact
    52861 gatcagaaac ggaatcccaa aaacaacttc tgcaatctcc cagcggcttt tgtgggctga
    52921 atcagggttc atcttctttc tcataatcac cgaatcgccc atcgctttca ccacctcaaa
    52981 ccccagccgt tcatagagat gcgccgccgg gtttccgttc cccacgctga gggagacata
    53041 cgcatactgc cctttggcgg cagccagcag gcgcatcagc agctctgtgc cgatcccttg
    53101 cccgcggtac tctggcagca ccgccatccc aacctcagga gtggcatcat tgatgtaccc
    53161 gtagccgtgt tcttcgcctt tgaataagcg catccaggcc gcccccagag gcttgccgct
    53221 ctcatcctca gcaatgaatc cgcagtcatg cacccgcccc cagttttcaa catatcgcgc
    53281 aatctcgggc tgccgcacaa tctgacgcgg caaagggtct ccacctgctg gcacatacag
    53341 agactgatac agcatctccc acagaaaggc ctgatcgtca ggcgttagcg gtcgaatgtg
    53401 atatttgggt aagggcttca ttaaacagcc tttgttgata caaaacgatg atgtcccgca
    53461 gcattttggt gtaacccaat ctcaaaaata attatacccc acaaccttga caaaccaccc
    53521 gaatcgccgt aagatatatc ttgtaaatct tacttatctt gcgaggtaac cccgtgcccg
    53581 taccccccga aggcaaacgc ttttatggcg caatcaccgt cagtgagcgc ggccaaattg
    53641 tcatcccggc cgacgcgcgc cgcgatttta atatccaggt cggcgataaa ctgctggttc
    53701 ttggcgatct cgagcagggc ctcgcgctga tcaaggcctc cgcgctgctg gagcgcagcc
    53761 ccgatttctt ccaattcttg ctgggcagcc gcccgcccga agcgccggcg cagttccccc
    53821 agccccccga ggaagcagaa tagtcaaaat ggacgcttta tctgtcagtc atcttgaaaa
    53881 aacctacccc gcctttcacc tgcgtgatgt ctcgtttagc gtgcagcccg ggcgcattat
    53941 gggcttcatc gggcgcaacg gcgccggtaa aaccaccacc atgaaatcta tcctcaacct
    54001 ggtgcatccc aatgccggct ccattcaatt cttcggcctg gatatgcccg cccacgagtt
    54061 tgaaatcaaa caacggatcg cctgcatctt tggcgccgcc aattattaca gtcaaagcct
    54121 gctgcagacc attgctgatg tgtaccgccg cttttacccc aactgggatg acgcccttta
    54181 ccgcacctac ctgcagcact ttgcgctgga ccccggcaaa aggctcaagc agctctcgca
    54241 gggcatgagc atcaaatttg ccctcaccct ggccctgtcg caccacgccg aactgctgat
    54301 cctcgatgaa cccaccagcg gcctggatcc cgtctcccgc gatgaactgc tgcatatctt
    54361 tgaagatatt gtggaccacg aaggtgccag catcttcttc tccacacaca tcacctcaga
    54421 cctggacaag aacgccgatg atatcctcta tatcaaagat ggtcagatca tcgcctgtga
    54481 ggaaaaagat gcctttgtcg cctcttaccg caaggtggat ggcctgaccg ccgacctgcc
    54541 cgccgatttg aaaaccagaa tcatcggttt ccacgagcgc aaaggtgaat tttccgggtt
    54601 ggtgcgcgtc accgatgccc ggggcgtcag cggcctgaat tttacccctg cagatctcga
    54661 atccatcatg gtctgcaccg aaagaggtta agacatgaaa aacctgctgt acaaagaatg
    54721 gaagctggcc atccacccca ccggttacat ttttctgctc tttggggtca tgctgttaat
    54781 ccccaattat ccctattttg tagccttctt ttaccagacc ctggccatct tcttcacctt
    54841 tatgaacggt aacaccacca acgatatctt ctttactgcc ctgctgcccg tgcgcaagcg
    54901 tgatgtggtc aaagcccgcc tgggtgccgt cattggcctg gaggtgctgc agatgctcat
    54961 tgctgtgccg tttgccatcc tgcgcagtca gatcatcccc accgagaacc tggccgggct
    55021 cgatgccagc ctggcgctgt ttgggctggt ctttatcatg tttggggtct tcaatgttgt
    55081 gtttctgccg cagttctatc gcaccggcta taagaccggc accccctacc tgctggctac
    55141 cctggtcatg acgctggtca tcgtagccgc cgaactggtc attcaactga cgcccgccct
    55201 caaacaggcc ctggataccc ctgcctgcac actgccacaa atcatcgtcc tgtttgccgg
    55261 gttgttaatc tttgccctgc tgaacctcgt tgcctatcgc cgttccgcca gcgccttcga
    55321 gcggctggac ctgtaaaccc cgccgcctta ccgtcgaaaa tcaaatccta cccggccgcg
    55381 ccacccaccc cgggcgccgc cgggtgattg ctttagccga tcagaatttt catcacgcgc
    55441 tgataaaacg cctcgcctaa aatatgcatt cccacaataa agggaaccgc caggacaatt
    55501 gccaccccaa aataaatcgc cagaccccaa agactaacaa cccctgcctc aagcagcttc
    55561 cagaacagaa taatgccata cggaatggcg atcagcaccg atgtgatgag cgccgggatg
    55621 tatctgcgca gaataatggc ctgcccaaca tgcataaatc catgcaggaa gaacagactg
    55681 ctggcgacca aaaatatcgg atagctttga aactcggcgg ctaaaaacgc tgaaatgcag
    55741 gttaacacaa agatcaaaaa aatcgggaaa gcaaattgcg tggtcgtctt gccgagaatg
    55801 attggcattt gttttgccag aaatgccggg gctttattcg ggatgcggct cataatcaat
    55861 cccgagttct tcttcagcca gggctcaaac agcaagatct cttcaaaatc atgcaggata
    55921 aatgcaatcg ggaataacca gatcaattgc tgaatatcag aaaacatatt gaacaccttt
    55981 cgaatcgatt aacgattttc cgtgccagaa gcaggcgtcg ggttccataa gaatagctct
    56041 tgcaaatcat ctggcaggtg gtctatcccc ataaattccc gcacgaccac agaatgaatg
    56101 agctgcctga cttcatcctt gacattcgtt atggtcattt gccagcgccc accaatgctc
    56161 cggcagtcat cttctatcaa aatatcaggg atcacctgct cagcaatatc tcgatactgc
    56221 tcatgttccc ctcggtagaa cagatattgc ccagaaaaca accctctccc aagtatctcg
    56281 cgaatcgcat taacatccgc caaccttctt cgagacgtga ggtatgcgat cctggcacct
    56341 tgtctttccc atgtgctgat tttatcggcg ctcttcccaa tcggagcata cctcttatcg
    56401 ttgagccaac cccaccaacg ctcaggcccc aggatggtac cttcagtaaa tataagaatt
    56461 ctagggttga gcctgtttcg ctccactgcc ccgattgaat aatctgcaat tgtttcgcct
    56521 aatttcaagt atgccatgta tgggttccct tattcagaaa ccgaatattt gtaaatctta
    56581 tcaccaatcg ccatccaaag atttttcagg ttatcttcta caatgtttcc tgatccggtt
    56641 gtaaaccaac agcccttatc tgacgtgggg tcataccacg ctgaaccatc gacccccaaa
    56701 aaatcctgat tttcatttgt gtaccaaaga taaccatttg aactttgaaa aattagcttt
    56761 ggatgcgccc agttataact ttcagtgtta agacctaagt gaatatactt caacctgttt
    56821 ggattcttta agacccaggt gccatctttc gataaatacc catgaatccc gatccatagg
    56881 tctccagaat cagtatatag ccaggtgccg tagtctggcc agcgaaataa tggaaccctg
    56941 attttttcaa cagtgttcga atcgggataa tatttcgcca attcgccagg taataatttc
    57001 caggatgcct gcttttcctg ataaaacaat aaaaacccgt ctggtgatgg aaccatactt
    57061 tgaacaatgc cgtcaaatga tgtttcataa tgtttaagtt ctgcatccat tggattgtag
    57121 ctgtacaacc catcttgctg ctgataaatc caaataacct catcgctttt gctgatgatt
    57181 acgtcatcct gtgggctcat gcttaaattt ccgtatccag caccatcaac ccaatacggt
    57241 aacccaatat cgtaaaattc aaacctgttt tcaaattcgt tatatttact aaacacagga
    57301 ataggttgat cccattccgt attattattt tggggataat tcaccccaac aatttccccg
    57361 gttgatattt caaataagtc tcgaacgatc acgtttaggt aagttttccc gctttgatcg
    57421 tatgggaagg gtgagataat cgaaaaattc tctgttccgg cgttgtgctt gataattttg
    57481 tcgtcagacc tgagccataa ttctgtttgc ccagaatatt ctctcaccag ttcgatattg
    57541 acacgagtaa cgaggccgtt tgatatctct tcgtaaaccg tttcaggtat ctccgcactt
    57601 aactcccatg gagcagaggg ataaatgtct gataagctgt agagctcatc atttctggta
    57661 atttttgcca tttgatataa gaaatcatca actgtaccaa tgcattgcca ttcatcttgg
    57721 tctgttgcca tcgcatctgc attgttttcc tgtgcaattg ggctacatga ggccaataat
    57781 aattgcaaac aaaccacaaa aatgagaaag tgttttttca tgttaccctc cacaagcctg
    57841 catgtttccc acgcgagcaa taattcatcc gtcgctgcgc cgatatttgc cagtaaattc
    57901 ttttaacccc ggcgttttct actattggtt aaatattcgt ctcgcgttat cttaaaagca
    57961 tatccaccct catcatccaa ccccagatag ttcattccaa tcttttccat cacccgtttg
    58021 gatgccagat tatcgggcgc cgcaccaccg tgaatgctgg ctaattccaa ttgaccaaaa
    58081 gcatagttca gcagcgctcc ggcggcctcg gtcgccagcc cctgccccca atagctgctt
    58141 ttcaacaggt agaacaaagc cgggtcggcg tctttcgggt ttgtgtggtc cagcccgcac
    58201 cacccgataa aatcattcgt cgccttgaag attatcgcca ggcaaagatg aacaattttc
    58261 cccggcctgt tctgcgcata atgggccatc atcgcttcga tttttagcac agcctctgcc
    58321 gtggaaattg ctccctcgtc caatttccaa ctgcaagcca cgccatccac atcgaccatc
    58381 gtcaccgttc gcagaatcag tcggggtgtt tcaatcacaa tggccggtgt catggttgat
    58441 ttattcaggc ccagcatcaa tgagcacctt atccggaacg ttccgggctt gttcgccatg
    58501 ctcaaaccga cgcggtccat agatcgccac aaccaccgcc gccatgccta gcaaaatgac
    58561 cgtctccagc ccgcgcgtct cacggataaa tggcagtgat gccgaagcat acatcaccat
    58621 cagatcatag accccgtgga acagcgaaac cagcaatata ctgccccggc tgttggcata
    58681 cagccaggta tagagcacag cgataatcac aatatcggcc ataaagaaaa gcaaggttat
    58741 tggagcactc ccggctttga acggccatag ctcgggcagg tgccacaacc cccacaccag
    58801 cccaaccatc aggcttgccg ccaggtctga atgccgcgcc cgcagccgcg gcagcaaata
    58861 accgcgccag ccaatttcct cccccgtaac cacaatccac cccagcagca gggacattaa
    58921 caaaaccggg ctggtgcgcg ccagtgtagc cagccacgga ccgatcattt tgatcgtctc
    58981 gggtttgccg ttgatcagcc catacaacgc aatcagcagc agcgtgccga tgaggggtaa
    59041 ggtcaccgcg gccaggtaga gcaggggatg aacccgccag gcaaaaaccc gcctcagcag
    59101 catctccagg gcctcgcgcc cttccagcag tccggtgagc agaatagccg caaaagctgg
    59161 cccaaagccg cccagccaat caagaaacag gacaggcttt tctccaagct ctgccaccca
    59221 ccacagcagc caggaaaggc caaaagccag aacaatataa gctgccagcg ggcggcgtga
    59281 gagtaagttg cgcaggtgca ttagattact tcatctgtat cttgccatca gaaaaatgat
    59341 atgttccatg atactgagcc gccttgcaca ttttttacac aggtcttgcc cggttcttca
    59401 taaaaactac cccggcaata aacccaagta ccagcatcac tacacctgct aaaacctgcc
    59461 cgccaatagg attgctcttt tggctgacct ctggcgtgcc cgtaggcgta atcatcggga
    59521 ccggcgtttc actcggtttt ggcgtgggtg ttttggcaac tatggttcgg gttggcgtag
    59581 cagacggaca taccaaaggt gtggccgatg gcgttggata taacacggac cagcgatcct
    59641 cttctttggc attagcaatc tcagcctgaa tttcgtggat gcgctgattt gttggatcta
    59701 acgccaaaac gacacccatt tcgtaattca ataattgttt agaattttcc agactcgtat
    59761 catcactcat ataataataa tcccataggt accaatcata atacatttcg agtaattcaa
    59821 ggtgcaggcg aatatcgtct ggatgtaaaa caagcgcctg ttgaattgtg atctctgcaa
    59881 gatggctcag ttgacctggt gggttaccca atttgacgaa ggaggctctt ttatatttat
    59941 ttgataattc aatggctaag tcaacgttac ccgggtcaat ccctatcctg gcctccatgt
    60001 ctagaatttc tcgccaataa tttggatgaa tgatcgtaac cccgatgatt tctcgtggat
    60061 taggttcgta attctgccag tgccaccgaa tctcatggcc aatttcgcta atgtgagcat
    60121 tgtcatgatc tggccaataa gcatcgatcg ttgctggaca gaccgtatat ggtagtcgta
    60181 gtgtaattgc cactgtccca atggactggt accaaccggc gccggttccc aggaaatatt
    60241 gaatttccgt gaagctgtcg ggatcaccta cgccgaagcc gtcctgaaga tccgtggtgt
    60301 aactcacccg aatattcaca acttgtccaa caggaaagtt gacatcaaag actgcccaat
    60361 cagcaggaga aaaactgtcg tccaaataag tttctgtaac aaattctgta ggtaccgaag
    60421 tttgattgac acttgccttg aaatatctgg caaccgggta attatcaccg ccccatccca
    60481 tggcattttc taatgggaag cggacgcgca tttgctcatc agtttccccg aggtttttca
    60541 tcgcaaaatc tgccgtaact tcaatgacat aaacatcggt tgaaacttct ttgaccacca
    60601 tcaagacatt ttctgccacc atttgaactt ttgtatcctg gcttgggttg atattgctgc
    60661 ccggtggtgg gtttggcgga gcaacatctg catgggttgg agtactcaag atcatcaaca
    60721 agagcattgt gagaatgaag gctgctgtct ttttcattgt ctcaaatcct atgtttgatt
    60781 cggttcatgg cgcgtgatta tttcttaccc gctgctgcaa ttcaattata ctattatacc
    60841 cactcagaat tactcccccg cgtaaaggaa aaccacgagg cgtgtttatc gccagggaat
    60901 ccgtataaag gttggacctc tgccagcgtc aatctttgcg ctggcagagg aatagctgat
    60961 tagcctactt cacttcatcc agcagccggc gctcgccctg caatttccga atttccgtga
    61021 cattctgcgt cacttccagc gtccccagaa acttcccctg cgggtcacgg acggcgaagt
    61081 agcggatatg gataaactgg ctctcctggc cttcctgcgg cttcgtctgg atccagaact
    61141 cagccgtgtc ccgtgtcccc gcgcgaaaat cctccaatat tttgaagaca atttgcacac
    61201 tgtctggatg gtggcagttt aagacattgc gcccaataat atccggcgtc cgctcaaaga
    61261 cccggttgtg cccgccagag taaaaccgca catcgttgcc ctcatccaca aacgtcacat
    61321 ccaccggaag atgggtaaag atcatggcga tctgctcggg cgtcattgcc cccacctcca
    61381 gcggaatgcg gttcttgggc tcctcattac acaggctgtt tgaattttgt gtctcagtca
    61441 taccatcctc cagttcaagc tgcaattggc aaacgaattt ctataaaatc catcttattc
    61501 gcttatattt taccatgccg ccgttgccgc ttgtgcatgg atagcttgct tttagacttc
    61561 aaggatcaat ccatcataag ccacttcata accatgctcc gccgcaaacg cggccagttc
    61621 cggatgcgcc ggattgccct catgggcaat atgagtggca aaggcgcgac cattggattt
    61681 taataaccct tcagatcgca tccgctgaac atgttcaata acttgatggg cgcttaagtg
    61741 atcgctccca ggttggcttg gcccgtaagt atgatccaag atgacaaggt caaatttcat
    61801 ttgaaattga tgaaatgctt gccaggtttc ttcaaagagg cttgccgtat cggtgccgta
    61861 aaaaatactg cgcccgttct cctcaactgc gaataacagg gcccccattc caggcgcatg
    61921 attcgccgga aaggcaatga tgcggtaatt gcccatgctg aagggttgta aaggttctat
    61981 ttggtgaagc ttaagcttta gccgtacttc agcctcgggc gataaaaggc tgtaatctgc
    62041 aaggtctctc tcaaagatct cggctgcctt ttccaaagtt tcagccgaag cgtaaaaatt
    62101 taggatgggc gtaccaagcg tgccatattc cgggctgcgt gacagcaagt gagacaaatc
    62161 gaaatggtca gcatgagaat gggtttgcaa acaataacgc acgttggtca aggtgcagcc
    62221 atggttttgc gccgccgcca tgatgtcagg attcaggtca ataagtaaat catcattgac
    62281 caacaggctg gaccgctttc ttaaactgct ccccccaagc aatcgtgcct gttcacaatt
    62341 tttacactta caaaatgctt ctggaaaagc attcgcagcc gcagttccaa gaaatgtgat
    62401 gatcatgtct tttcctattc ctaatctttt tacggcaggc taagttaaca accaataggc
    62461 gggtagaagc gaggcgtcgg attcgctgcg ctgttcagtg tctcagccac cacaggctcc
    62521 atctttggat ggttcatcgc attgattgac atctctttaa gcacaacctc atgctcaggt
    62581 gtcacaatct ggtagcaatg gttatcgcca ccacgcagaa cgcggatctg ttgaacaggg
    62641 cccatcctct tcttccttca aagctggttg tccaatggct tgcgttattg gttggcatta
    62701 taatcctggt gacaccttaa acgtagttac atgg
//
LOCUS       DAOPVS010000004        45418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_755_length_45418_cov_59.706787, whole genome shotgun sequence.
ACCESSION   DAOPVS010000004 DAOPVS010000000
VERSION     DAOPVS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 45418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45418
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_45418_cov_59.706787"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..>1803
                     /locus_tag="PKL11_00995"
     CDS             85..>1803
                     /locus_tag="PKL11_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12201.1"
                     /translation="MKTLRRSCCVIFMILWVLSLIMPGTGVQARTLSTVTISGIITDG
                     GGHGYPLYARVSLEDGETAYIAYTDPFSGAYSLNVTGDVTYQMHIEAVIPGYLAEETE
                     ITPATNETVNAALEVDAFRCIAQGYQRVIYGGGTFENFDGVPAPPMIPGEWGQVDTLG
                     TGGNWATATNTQQPAGFPPYSSPNLAYFNSYSAPDIRSRLYTLAGTDASTSDGVMVEV
                     QVFHDTGLPYNDDRLQMQYSLDGVNWVNVGEPIHRYDGSNGWKQHMVTLPAGAAVYLG
                     IEGISEWGYDIHIDSIDWNPYTCEPEDHGGIVAGYVTDSTTGEPLVGRVLGNEISNTL
                     FIDEDPEHAGLYWLFDTIDHKDPAVITASAYPWYLPETANVDVIENGLVRVDFALEPA
                     PLQTISGVAADGSGHTYPLYTQIHVTGNLDGLPGNGFEFDQWVYTDPFTGEYQITVPD
                     QHTYGLETETFFDGYDAVLVEATVNGSPLTQDFHLQASAGCTAPGYTPLKAAQPDLTE
                     VMADCVPQSGGLVGGYINDGNTGLPVIGAAVSSDGVPGFNVQSVWWGTDSAHAGVYLA
                     FQRNPPA"
     assembly_gap    1804..1903
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1904..3348
                     /locus_tag="PKL11_01000"
     CDS             <1904..3348
                     /locus_tag="PKL11_01000"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNZ12202.1"
                     /translation="MLVHLDLATGASVPIGPTGVNGTEVQGLDFDEATGTLYWSASIT
                     PAESKLYTISPITGAATLVGSIGASQLVGCLAVASFNPPNVSWLSLGQTTGTLAAGES
                     ISIPVNFAPDVLLPGIYEAGLLLVDNTPYMPILGGNFIQGRGRIPITMTVEPPLFEKS
                     ASPETTHGSQLTYTLSFDPLAATGTIAQIVDELPAGVTYVDGSLTVSSGYASYNPGTN
                     TIVWASVPPKSTKDQGTIVETLVTITFDVMVTAPRGETISNRADLSYGGYVADRTVDT
                     VVVNAPPSADDQAFSTDEDVDYSGTVAGSDADGDTLTYGTISEPEHGEITLNAETGAF
                     TYSPDDNYNGSDGFTFSVADGYGGTDTGTINITITPVNDAPIPPAVSDVQWVMNAAHT
                     YAIPPFEDPDGDALTYTASLSGGGNLPEWLSFDDQTRTFSGTPGEADLGDYPIEITAA
                     DPDVATGTTTFTLKVVAELMVNIYLPAILR"
     gene            3571..6015
                     /locus_tag="PKL11_01005"
     CDS             3571..6015
                     /locus_tag="PKL11_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ12203.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPE
                     GVVPQILEILKVDPTQLVERLDYILRTSPKASIFGAGAGQIFITPRVKRIIDLANEEA
                     SRLKDEYISTEHIFLAILSERNTPAARLLESAGITRERVNEAIGQLRGGQRVTDPQAE
                     TRYRVLEKYSRDLTQLAREGKLDPVIGRDQEIRRVMQILSRRTKNNPILIGEAGVGKT
                     AIVEGLAQKIASNDVPEILMGRRVIALDLGAMIAGSRFRGEFEERLKAAIEEVQRSEG
                     EIILFIDELHTVVGAGAAQGAMDASNMLKPALARGELQCVGATTLDEFHKYIEKDSAL
                     ERRFAPVYVDEPTVDDTIKMLFGLRDRYEAHHKVHFADDALVSAARLSSQYVTDRRLP
                     DKAIDLMDEAAAKLRVALYLMPPDLKQKKTELDRLRAEEEQAGLERDYERAARKKAER
                     IRLETEFNEERDRWEEEHKLDEVVDVNDIAEVVAQWTGIPISQMMENEAERLLHMEDR
                     LHERIIGQEDAIHAISDAIRRARSGLKDPQRPIGSFIFIGPSGVGKTELAKALAEFMF
                     GDAEALVRLDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDEIEK
                     AHPEVWNALLQILDDGRLTDGQGNVVDFRNTVLIMTSNLGTEFVQRGGTLGFLTADTT
                     GEERQTHDKIEKALKSTFRPEFLNRIDEIIMFAPLSLDEMRLIVDLQMKEVSTRLEEH
                     GLHVALSPAAREWLAQVGYDPAFGARPLRRALQKHVESPLSVSLLSGQFAQGDTVLVD
                     LDTEKNALTFLKADQA"
     gene            6114..7184
                     /gene="gmd"
                     /locus_tag="PKL11_01010"
     CDS             6114..7184
                     /gene="gmd"
                     /locus_tag="PKL11_01010"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ12204.1"
                     /translation="MKKALITGITGQDGSYLAELLLSKGYEVHGIIRRASTFNTRRVD
                     HLYHDPHANGEDVKLYLHYGDVSVGETLLNVIYNVRPDEIYHLAAQSHVRVSFDMPEY
                     TGDITGLGTIRILDAIRKSGTEARFYQASSSEMFGGAKPPQNEETPFEPRSPYAAAKV
                     YAYWVTRNYREGYKMFACNGILFNHESPRRGETFVTRKITRALAAIRAGKQKHLYLGN
                     LDSRRDWGYAPEYVEVMWRMLQQDQPKDYVIGTGESHSIREFLDEAFGYAGMDWHQFV
                     EIDPRYFRPTEVDYLLSDPSRSRNELGWEPKVRFHELVRIMVDADLELQGLPCPGEGR
                     KIIEDRFGGWHRWEHQVVSMER"
     gene            complement(7286..7975)
                     /gene="rsmI"
                     /locus_tag="PKL11_01015"
     CDS             complement(7286..7975)
                     /gene="rsmI"
                     /locus_tag="PKL11_01015"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNZ12205.1"
                     /translation="MMKPPGTLYIVATPIGNPGDITQRALEVLASVDAIICEELRPAT
                     TLLKRLEITPRQLLTLNEHNEAERVDEIVNRLAMGESFALISDCGTPVFADPGHALVR
                     RTAELEFRVQPIPGVSSLMTALSLLDFKLERFVFGGFLARQPDQRRQELQRLRSLRMP
                     IVLMDTPYRLGALLEEVSRAFGANHSILLAMDLTLPSEAILRGSITEVRQQVGARKAE
                     FILIVYAPADR"
     gene            complement(7972..8817)
                     /locus_tag="PKL11_01020"
     CDS             complement(7972..8817)
                     /locus_tag="PKL11_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNZ12206.1"
                     /translation="MLDALLQPLTYAFMQRGLLAAILVGIICAVIGCYVVLRSMAFLG
                     DALAHAILPGVAVAYLIGGNLLIGALAAAVLVALSIGFLSRRGDLKEDTAIGILFSAA
                     LALGVALISSIRTYAVDLSHILFGDVLGVTPADLWLTAGLGVVVIGLVAFFYRPFLVM
                     AFDPVLATTLRWRTGLLRNLLLVLLALTIVISMQTVGIGLVAAMLVTPAATAYLLTRR
                     LRVMMLLSAGIGAFSGIVGLYVSYYLNIASGAAIVLSATLFFLLAFFLAPQRGLLWQV
                     LRRHA"
     gene            complement(8810..9634)
                     /locus_tag="PKL11_01025"
     CDS             complement(8810..9634)
                     /locus_tag="PKL11_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNZ12207.1"
                     /translation="MSESQKQPLADNPARLELDHVTVGYNGRAALQDITFDVPHGAQV
                     AVVGPNGAGKSTLFKVLVGLLPVRAGQIRIHGQPLGHHHDCVAYLPQREEVDWRFPVT
                     VWDVVMMGRYGRAGWLRRPSASDRAAVLRALEQLNIKAIARSPIGELSGGQQQRAFLA
                     RALAQEPHILLMDEPFTGVDSATQEATLNLLDDLRQSGVTTLVSTHDLKLAAERFERT
                     LLLNHRLIAYGPSEQVFTHQHVAEAFGGQLLSLGGSAEIVVDQCCPHEHDEEHTHA"
     gene            9722..10708
                     /locus_tag="PKL11_01030"
     CDS             9722..10708
                     /locus_tag="PKL11_01030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12208.1"
                     /translation="MMSAPTQLDHLREVDARRDLNAIANLIDICFWAHLDPEGRAYLN
                     RLRRLAVQLQVVPLRSMANAAVPIQGFVWEEKGEVVGNLTLIAFRSRGQLLYLIANVA
                     VHPDYRQKGIGRALTQRALDYIRATGAASAWLHVRADNPTARHLYDTLKFEERCQRVN
                     WVWRPEDQVPVVKPPAGIAVGTRRKAEWGQQRAWLQSNYPAAVDWNLMFDAQRLKPGE
                     LRSLWRWLNSANQTHLTVRRDDQPLGLLTWETGAGQHSFFWLAAPDQPDEAALTWLLM
                     QARHEWTTRARFYNAALRPMQINYAAGQAAAAFEAAGFSAENHLVWMEHPLR"
     gene            10761..11144
                     /locus_tag="PKL11_01035"
     CDS             10761..11144
                     /locus_tag="PKL11_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNZ12209.1"
                     /translation="MKKFLIRLAVNAVALYAAVALLKDTYIYPQSENWTSIIWLALIF
                     GLVNAILRPIVITVGCPVIILTLGLGTLLVNTLMFWLAGQIGLRFDVGFEVEGFWGAF
                     LGALIVSVVSFILSLIFRDARKSRD"
     gene            complement(11213..14638)
                     /locus_tag="PKL11_01040"
     CDS             complement(11213..14638)
                     /locus_tag="PKL11_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ12210.1"
                     /translation="MSEKSESWLDRPLTAYARWLTVENLLAVLIILIALVSRLYGVGW
                     RAMSHDEVNHVVPAFDLYEGRGYRHDPITHGPMQFHLLALSYTLFGDNDFSARIPAAL
                     FSTAAVAFVLFGFRRYLGRKGALFAGIFFLISPYMLFYGRYTRNEGFIELLGVMMLFA
                     VLRYLDNGKPSSLYILTAALVLHFCTKETSFIYAAQLLLFLALLFLVRIAQTRWPEDR
                     RRTLFIVLMAVALSLIIIGVGLGTYEADLTKQAALDPASVSPQLINTILTATLFCVGL
                     AIISGVIALVTLVRSLGLKAIRAERSFDLLILVGTLVLPQLTAFPIKMLGGNPLDYTS
                     TAGIITIIVTLVILLVISGLIGMWWNAPLWIGNAVLFYAVFAVLYTTFFTNGYGFFSG
                     IVGSLGYWLSQQGEQRGSQPWYYYAFLQIPMYEYLAALGTLLAAYLGFRHNRLSALPG
                     DSPTAEPPQPQPLPAPIASEANDPTPAPEPVARPAKVPTLALLLFWSLTALVAYSVAG
                     EKMPWLTVHITLPMLLASGWSVGWLADKIDWQSLRNKEGLASVFMIAAFLLGVANLLG
                     SVLGLNPPFQKPLTGNTLTTEQLSATYAFVFWSIVTVFCAWGTFNLLRKWRTVEILRL
                     TALVVFVFLAVLTTRHAYRASFINYDNAKEFLVYAHASGDFKDVVEQVEEISRRTTGG
                     RDAIVAYDNDVLYGGWWYFRDYPNKRWFTDPTRDLETADMIVVGEANFSRLEPIVKDQ
                     FIGFDYMRLWWPMQDYYGLTWQRIGNALKSPQLRTGLYNIWLNRDYDLYAETTGKTNL
                     TLENWQPSARMRLYVRRDIAAQIWNYGVAAAAAPEPEVDPYQEKIQVLSPDIVVGVGG
                     DPVQLTNPHGLAVAPDGSLYVADSRAHRILHFSADGETLLNSWGQFAAVEAGVQPAGG
                     YFNEPWGVAVGPDGSVYVTDTWNYRIQKFTADGTFLTAWGTTDPAAGGPVFYGPRGIA
                     VDSDGRVYVTDTGNKRVVVFTSEGQTITEFGTAGLSLGEFDEPVGIAVTVDGRVMVAD
                     TWNRRVQIFLPNENHTAFTPLSAFDISGWKGQDVENKPFLAIDNRGHIFVTDPPNYRV
                     LEFTDTGEFVGGWGGYSTLSDGFANPSAVVVDAQGHAWVSDSANNLILRFTLP"
     gene            14782..15975
                     /locus_tag="PKL11_01045"
     CDS             14782..15975
                     /locus_tag="PKL11_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12211.1"
                     /translation="MMPSKPARYSLFALLYFSQGAVLSFFTAFNAIYLLSHDVSMTRI
                     GLMGTIALLPFIFKIFLGMLSDRVNLLGHGHRKPYILIGLVVQAVCLLVVPQVNPGTH
                     FWLYVALAFLLQLGMALYDTCTDGLALDTTPPEEEGTIQGFMVGGRALGVVLIAGIIG
                     LVADRFSWAVAFYTLAALSLLPIPLLLKAREIKQSEGRRFEWGAFKAFAQPAVIGLGL
                     LGALYSLIINGANEIVNPFLQSRFSISTTQAGFLTAVWGLGVVLGGITGGRLTDRLGH
                     ARATQVAMIMALAAIVPLAFIPSAWLAWGLVAVFGLAYGFYETVYFAISMNYTDRRIA
                     ASMFALLMAVANIGTGIGLGISGALVDSLGYPITFIILAGLNLLAIPLLTMIFGRATE
                     KVQEY"
     gene            15979..16509
                     /locus_tag="PKL11_01050"
     CDS             15979..16509
                     /locus_tag="PKL11_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNZ12212.1"
                     /translation="MPTNLPEMPFPNSYWVEPGRFMAGEYPGVYEVSHSKLRLERLLK
                     AGVSVFFDLTQENDARPYLPLLNETAALLGRKIHYIRMPIVDFFIPTVEEMKIMLNQI
                     DAALAARQTIYVHCIGGLGRTGTVVGCYLVRHGQNGEQALLSIQGLREHVPNWWESSP
                     ESPAQREMVRNWKPGQ"
     gene            complement(16518..16955)
                     /locus_tag="PKL11_01055"
     CDS             complement(16518..16955)
                     /locus_tag="PKL11_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12213.1"
                     /translation="MSNLFGSPGFKSLVEKYCKQQDWKIAEIDDKHATIRFNMESGRV
                     QTLYIIKYDNTLEFSVPSLAAFDSEDKIPHFLSTILLKKSATQKIGFWTIEEIGGKHV
                     YSVMQNVEMQLLEAEYFTKVVRALVSQCDDFEGLLLKMLEDNK"
     gene            17185..17763
                     /gene="rplI"
                     /locus_tag="PKL11_01060"
     CDS             17185..17763
                     /gene="rplI"
                     /locus_tag="PKL11_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNZ12214.1"
                     /translation="MPKGLEKMKVLLIKDVYKLGRAGDVRRVADGYGRNFLLPQGFAI
                     LATQGALKQAETIRRKAEARRIVLNNEMSGIANLVSGLVLNFTSKAGETGKLYGSITT
                     QMIADAINEKLETKIDRRQIECQPIRTLGEHKAHIRLTIDLIPEVKIVVQREGERKAA
                     PAVKPQPEVVETTPAEVPAEPAAEEVEEEAAE"
     gene            17846..19198
                     /locus_tag="PKL11_01065"
     CDS             17846..19198
                     /locus_tag="PKL11_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNZ12215.1"
                     /translation="MSPTIGAQLKQAREARGLTLEQAAHATRLRLHYLRALEEDQREV
                     LPSEVQGRGFLRLYADFLGLPVQPLLDAWKTNQVVIEALPPAKVEAPTVTEPVKEESV
                     APEVVIAPEPPPPAVINPPTTPRAEIIAPTAESRQILQQIGAELKQQREGLSLSLADI
                     EKHIHVRQHYLKALEEGRFEVVPSPVQARGMLSNYASFLNLDTENMLLRFAEALQTRR
                     TENLPAAPQGRGAAKPRAAKAPGWTRFLTPDLLIGGTLVIVLVVVIIWSTMNISAIGT
                     TPATQTAPSISDVLLTTTPLALPTSDLPVTESTGISPEMPGDATVPTLTLAPLNTDPM
                     QIVVIGLQRTYLKVTVDGAEVFSGRVVPGNAYNFSGSEQIELIAGNAAGVQIFYNQSD
                     LGTLGAIGQVVSFIFTPGEVITPTPSFTATSPATPTPTPTPTLTPTPGATPTVTQFVP
                     "
     gene            19202..20530
                     /gene="rimO"
                     /locus_tag="PKL11_01070"
     CDS             19202..20530
                     /gene="rimO"
                     /locus_tag="PKL11_01070"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNZ12216.1"
                     /translation="MKANTFYLVSLGCAKNTVDSTSMAQLMESGGYRAVEKPGQANIL
                     IVNTCGFIQPAREESLQELRTLAKRKKPGQILIAAGCLAERHREQLAREVPGLDGILS
                     TRRWMDVLNMVQHLRSEGHPQQPYDLPEAPDVGREDHGVLRSALQGSSAYLKIADGCR
                     RPCAFCSIPLIKGPAVSRRPEQIIAEAQTLADMGAQELILIAQDTTDYGSDLGLKDGL
                     AALLEELTRAVPGVPWIRVLYAYPGYVTDRLIEVMAGHPQILPYLDMPLQHAHPDTLL
                     RMRRPANMEWVHRTLEKMRKALPALALRTTFIVGYPGETEAEFQTLLDFVNEIQFDRV
                     GVFPFSFEPGTTSEPLGDPIPTEVKEERIERLMTLQSGISLERNQTWVGKTLPVLTEG
                     SNDGITVGRSYRDAPEIDGLVFVDGELPIGQIQTVRITGALTYDLTGEPI"
     gene            20541..22049
                     /locus_tag="PKL11_01075"
     CDS             20541..22049
                     /locus_tag="PKL11_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ12217.1"
                     /translation="MSRTDWLALLLAILTFLGAAWITDQVFEGLPHLEDEMAYVWQAQ
                     AIEGGKLLLPTPPCPRCFLQPFVVDYGGMRTGKYPPGWPTLLAVALRLGLQDRINPLL
                     AGLAIWLVYRLGKKITDERTGILAAFLTATSPLFLMNSGALLSHPWTLVLSATVALGW
                     LDSFVIPNKLPRAVPLTAAALALGALALTRPLTAVGVALPFIIHALVLFIRGPREVRW
                     RLVGFAALAGSLAGLHFLWQAAVTGNPLTNPYTLWWPYDQIGFGPDVGLQEGGYQPIH
                     AWYTTSYNLWVAARDMLGWGTVSWLMLPFGLLALRRNKNALLVGSVLPALVLTYAFYW
                     IAAWVLGPRYYFEGLYSWTIFTAAGIRWLAGQPFAKTASRWKQWGQKARYAAVTALTI
                     CLIAANLIYYTPQRLGALQGLYSVSATHLDPFRAAETKGMTPALIMVHPVDSWIEYGR
                     LLSLSSPYLNTPFIFTVSRGPEKDAQVAALYPDRKVYHYYADTPWVFYEAPR"
     gene            complement(22046..23266)
                     /locus_tag="PKL11_01080"
     CDS             complement(22046..23266)
                     /locus_tag="PKL11_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12218.1"
                     /translation="MSDKDPAETVPSRLNQSQAVTRPTPLASSPLETLPVKPEITEEP
                     PAPPPAEIEPPTAEPPAKPRRSRWFWLGLLIVLLAAALSAWVGYRAGIARRLAAEQEQ
                     RVMLASIQYGLAMQDIESGRLDAARQRLEYVIETDPTFPDAQQKLMDVMLAQAQLKTP
                     TPAPILTPTPQFTPTPDLRGEVDLYQSVLNHMAAQEWDAALTTLDQLRDTNLNYRTVD
                     VDGLYYICLRFRGAQRILYEGNLELGVYDLTLSEQFSQLDEEALGYRNWARYYMAGAS
                     FWRVDWVRVINYFSDIVPYVPNLRDSSGYTAGERYRLALIGYGDQLAAAGDYCEAQKQ
                     YEEALTLAPDPVLQPTLQDVVDRCERGEGEEDGGEDSPPAETPPPDSTPTETPPTEVP
                     TEVPTEIPGVTPTP"
     gene            complement(23285..>23725)
                     /locus_tag="PKL11_01085"
     CDS             complement(23285..>23725)
                     /locus_tag="PKL11_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNZ12219.1"
                     /translation="TRGYFAALTGHALMEVASRSHYAAQDARTPMRTGALNLTVYLVL
                     GLTLSNFLGAPGLSLAEAGAFTSQAVLLYIFLNRKLPQNLQVGSSLVRGGLAAVLGAG
                     LALGVLWLAAPLLGQTIASVAAMLAGAALALVIVWREVRLLLHL"
     assembly_gap    23726..23825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23826..24511)
                     /locus_tag="PKL11_01090"
     CDS             complement(<23826..24511)
                     /locus_tag="PKL11_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNZ12220.1"
                     /translation="MDNSKPHTNRVTRAALLLAGLFAIDKVVAFVRQVLIARQFGLSS
                     ELDAFNIANNVPDMLFLLISGGGLAMAFIPVLSETLSRDGRRAAWDLFSRIANLAFLA
                     TLVMAAIIALLADVIVRSAVASGFTPDQQHLVINLMRLNLIATVIFSISGLVVAGLQH
                     NQHFLLPALAPILYNIGQIIGALFFSPTEGLTIAGVTLPTLGLGVQGLVYGVILGALM
                     HLGIQIPGLAR"
     gene            complement(24504..25316)
                     /locus_tag="PKL11_01095"
     CDS             complement(24504..25316)
                     /locus_tag="PKL11_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNZ12221.1"
                     /translation="MIAFMESLHPVVQALLATLFTWGVTALGSAGVFLTKQVDRRLLN
                     AMLGFAAGVMIAASYWSLLAPAIEMSAGMGVPEWMPAVIGFLAGAGFLFAVDKTLPHI
                     HLNIPGFKAEGVKTKWQRSILLMLAITLHNFPEGLAVGVAFGAAAAGLQEATVAGAIA
                     LALGIGLQNFPEGLAVSAPLRREGLSRRRSFFFGQLSAVAEPIAGVIGAAAVLLMRSL
                     LPYALAFAAGAMIYVVVEELIPEAQQGEHTDVSTIGVMLGFAVMMLLDVALG"
     gene            complement(25392..25733)
                     /locus_tag="PKL11_01100"
     CDS             complement(25392..25733)
                     /locus_tag="PKL11_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNZ12222.1"
                     /translation="MDRNTVVLFTRFGLGHGPEALQQALAVKFLTLTLESGQLPARIL
                     FYTDGVRLACTGSPVLSQLNELAARGVELVLCKTCLDTYNLADDVQVGIVGGMGDILE
                     SLQQAGKVISL"
     gene            25993..27075
                     /locus_tag="PKL11_01105"
     CDS             25993..27075
                     /locus_tag="PKL11_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ12223.1"
                     /translation="MQTYPIPMVPGPVSVPPEILAEYQVDFGSADLEPEFLALYQQTE
                     ASLQKVMRTKNSVAIQSGEGMLALWGALKSCLQPGDRVLSVATGVFGYGIGDLARTVG
                     AEVRTIGLGHDQTIENWEEIEQAILEFRPKMITAVHCETPSGTLNPLAQLGELKRRHQ
                     VPLLYVDAVASIGGAPVLTDAWQIDLCLGGSQKALSAPPEMCFLSISEAAWEIIEAVG
                     YAGYDALKPFRTAVRDFYFPYTPSWHGVAALNVAAGRLLHEGLEYAWARHEAVAQYCR
                     ERVEKMGLKLFPAAGAIPAPTVTAVYVPKNLSWCELDKRFRAHGLVVGGSYGPLAEKV
                     FRLGHMGSQANRELVSRALDVIAAVI"
     gene            complement(27132..27923)
                     /locus_tag="PKL11_01110"
     CDS             complement(27132..27923)
                     /locus_tag="PKL11_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ12224.1"
                     /translation="MPLGWKDFSTTSFNALLLLERVQILWLPVGNVPIPSLAAALHGN
                     PAVLWFLQHKCPEISPWLAEIMSAHPAPENPAALRTAEVQVLQALEDWLVYVLEPECY
                     DRQPFTRWDSAELLNMVDFSGKTVIDVGAGTGRLALTVAPLARLVYAVEPVTNLRDYM
                     RTRAAALGLTNFYAVDGLITTLPFPAAFADITMGGHVFGDELTAEWQELVRVTRPGGL
                     ILLCPGNNDKDNPTHQFLMAQGCQWSVFEEPTDGLKRKYWNQLPG"
     gene            28036..29031
                     /locus_tag="PKL11_01115"
     CDS             28036..29031
                     /locus_tag="PKL11_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12225.1"
                     /translation="MKTRLWLTIGLVLMALTISACFCSMPDEDCPDGMYYDDEYEECV
                     YEDEEDATNADPTDWDTEEGDDSDDGNVFEDPSDDEASAYDDELVLAEYQVDGDELYN
                     ADLPSDIPDELVKYQEDEDAHYEAWEVFTQLMPQEYRESVTSFAIFTDGESETLAWVE
                     PVTDDLSEWRLGLDVIDVESDPDEFLYTLLHEYSHIFTLSPPQVDLGGGTCSYYETDE
                     GCTEPDSYLSAFYEQFWTDIWDEWQAFQDEPDESLYEDYLTEFYEAHEDEFITEYAAT
                     DPSEDIAECWSYFIMDPRPNGDTIAEEKILFFYDYPEMVEMRDEIQARLEEYFGR"
     gene            29098..29529
                     /locus_tag="PKL11_01120"
     CDS             29098..29529
                     /locus_tag="PKL11_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNZ12226.1"
                     /translation="MNTRLTRSKTERMLGGVCVGLGRYLKIDPVFVRLFFILLAIGDG
                     IGVLLYLILWLVLPEESAEEASLADRARQMGADFGTAISHPNPKTGMYLGIGLIVLGA
                     VYLLNNLNIPGLAWLNLHYLWPVLLILGGLVLLLRRDQSQP"
     gene            29639..30637
                     /locus_tag="PKL11_01125"
     CDS             29639..30637
                     /locus_tag="PKL11_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12227.1"
                     /translation="MKLIYENDLSEAGEFKRFARQAETAAFLGSPALCLDGLVVFPNL
                     ELTCGLIEVEIAAEKSCYPGVAFRVQDQGNYELAYAQPHTSGQWDAIQYDPVFHGTNT
                     WQLYHGAAYQQTAQVPTHEWVRLRVGFTENRMVFWVNEQPPLLVEHLAHPTRAGWMGL
                     WTYLPAYYRNLKIWDAPQFPEQPGIAPNCPSGTVTEWQVEGYGVIPCEPNGSLNVSRY
                     LPISAQQVQLKRSFEISAGEVLVNGPVNLGFGFSDTLALELDGQVIYQGENKFGGFET
                     RESRGYVDQKSHHLALEIAPGQHTLTATLGISEYFGWGLALAMEGPAIHWLPAGLE"
     gene            complement(30665..32299)
                     /locus_tag="PKL11_01130"
     CDS             complement(30665..32299)
                     /locus_tag="PKL11_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ12228.1"
                     /translation="MLSVSNISKSFGLDTVLSKINFTVNSGERLGLVGPNGCGKTTLM
                     RIIMGLEKPDEGGFHFIPSSLQVGYLPQGFTYPPGETIARFIARMEGDLPALTAELEE
                     LAPRLTTPEHQQRYDQLLNLIQVAAENAGRTASVLAALGVDNLPPDQLSQSLSGGQKT
                     RLALAGVLISNPQLLLLDEPTNHLDLNMLEWLENWLLSFRGGVLLVSHDRALLDQVAT
                     GILELDPLTHTLRAYAGNYADYLEQKLIEREHQWQAFQDQQVEIARLEQSARNIRGLA
                     KFRKGGKADTGDKFAKGFFANRSLGTMRRAKALEARLEYLRTAGRVDRPSRTWQMKVA
                     FGATPPSGKDVLATEELAIGYANGPLLSDLNLRLRLGERVALIGPNGSGKTTLLRTIA
                     GVLPPLSGQVRLGVNVRLGYLRQEQEDLDPTLNALETIQRLAGFPETEARQFLSLYLF
                     TQDDVFTPVGKLSYGERARLSLGALVVSGCNFLLLDEPINHLDIPSRARFEQALTTFE
                     GTALAVVHDRYFIDGFATTLWQVENNGIRVIYGAEH"
     gene            complement(32511..32693)
                     /locus_tag="PKL11_01135"
     CDS             complement(32511..32693)
                     /locus_tag="PKL11_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12229.1"
                     /translation="MVRLELSPEQYQLLVELLESCLSDLRMEIVDTDRIDYKDMLKDR
                     KDVLLSLLETLRSASA"
     gene            complement(32786..33685)
                     /locus_tag="PKL11_01140"
     CDS             complement(32786..33685)
                     /locus_tag="PKL11_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12230.1"
                     /translation="MPGQKNNPSRRLSRPIHLRWLVTRLLLIFVLAWTFSLGGLSAIY
                     VRSLMVPPCPASLAERPGYQSVTIATSDGYHLRGWWRPPQNGAVILLLAGNGGSRDSM
                     LGDAELLARHGYGILTLEYRNCQGGAATLGYREATDLAAMLDFAQSQPGVAWVGVLGF
                     SAGGAAAILGAADLPGIDAVVAEGHYASLHYEITNANSPVLSPAWQVQQWVVGLFWVA
                     LGHSPRQISPLDTLPHLAPRPVLLIFGEFEADNNRAFDQLAAAGPAAQMWIVPGAGHG
                     GYAQLAPLEYEQRIIAFFDSARR"
     gene            complement(33666..34706)
                     /locus_tag="PKL11_01145"
     CDS             complement(33666..34706)
                     /locus_tag="PKL11_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNZ12231.1"
                     /translation="MDVAPEVLAAQARPILPFRSSEFENFFRATAEKAQPLFGGGGRV
                     FQGSFSGTGMQEASIRNFVSSAVLICVNGASSERWYDIAIANGKSADRLDADWGQPIL
                     PGELTTALAQRQYDAVAIVHNETSTGVENPLPELAAAVRTASPDTLILVDAVSSFGGV
                     RLEMDPWGIDFLFAASQHCLALPPGLSLAAASDRAMQRAEAVPDRGWYFDLVRMERHR
                     LKDSLPSTPAMPLLYALDAQLDRMMAEGFEARYERHAALSARVREWAAGHNLPTLAPE
                     PYTSKTSTVLRNADNWDMTDLNKFLLQRGMRIANGYGRLRETTFRIATMGELQPADVE
                     NLLRALDAWAEK"
     gene            complement(34748..34945)
                     /locus_tag="PKL11_01150"
     CDS             complement(34748..34945)
                     /locus_tag="PKL11_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12232.1"
                     /translation="MSKQQKRNTNGISQTSPTPVAPVTTAARTTEFNPNYDYVIKDLR
                     RIGLLAGSFFVILIVLSFFLR"
     gene            complement(34970..35887)
                     /locus_tag="PKL11_01155"
     CDS             complement(34970..35887)
                     /locus_tag="PKL11_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HNZ12233.1"
                     /translation="MSRHRRVEDLTVDELRQLLIEKRRAERRARIDHFRRTGRMILVE
                     PLPAPAPIDGLRSIPTEEADLPAGAPAAPSKRQRVLNSFLSAIEVLAVIGLIFILFNG
                     MNILRNLNSEVAALIEQPTLTPTPLVMAVVLPSGHTPPNAVDGGGRPNEAEIPEHLRP
                     LAQTLANLPVPTPGPEQAQRLQIPDIDVDAPVVQGDGDEQLKKGVGQNIYSVNPGQNG
                     NLILAAHNDVYGEIFRHLDKLEPGDQIIVYTSLRAYVYVVEQTQIVEPTRVEVMAQTT
                     EPIVTLISCYPYLKDNMRIVVTASLLEQK"
     gene            complement(35936..36316)
                     /locus_tag="PKL11_01160"
     CDS             complement(35936..36316)
                     /locus_tag="PKL11_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNZ12234.1"
                     /translation="MPAPCWRLVPVGWAPVLASKLFRSLKTSEFPAPSSRPPTIPCNE
                     CPAGQMHLQNVTYFTWLADQLITVSDFPAWVCDVCGRREYDPRALNQLSLILSPNAGR
                     AMTRRRGPVKRAHPRRKAPRSNHP"
     gene            complement(36231..36974)
                     /gene="deoC"
                     /locus_tag="PKL11_01165"
     CDS             complement(36231..36974)
                     /gene="deoC"
                     /locus_tag="PKL11_01165"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNZ12235.1"
                     /translation="MKLNQLLQLAQSYEAQLPPFQPEYAADPAGLAGMIDHTLLKPEA
                     TPAQVEALCAEAVQYRFAAVCVNPIYVDLVARRLAGSPVATCTVVGFPLGAVPAETKA
                     FETRQAVEAGAREIDLVIPVGLLKAGEAQAVHDHIAAVVQAAKPGGALVKVIIETALL
                     TRSEKILACLLSRAAGANFVKTSTGFAASGATPEDVELMRRVVGLDLGVKASGGVRSL
                     ADARAMLAAGASRLGTSSGIKIVQESQNV"
     gene            complement(36971..37567)
                     /gene="lepB"
                     /locus_tag="PKL11_01170"
     CDS             complement(36971..37567)
                     /gene="lepB"
                     /locus_tag="PKL11_01170"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ12236.1"
                     /translation="MEDYRAEPVAQDPPEVKKKPAWKTFLSEVLQTLLLAAVLYFLID
                     SMVGRVRVENISMQPTLKADELLLVNKLAYRLGNMHHGDIVVFHYPGNPTEDYIKRLI
                     GLPGDEVRVENGQVLVNGYALTEPYIAAPPSYTGVWNVPEGSVFVLGDNRNQSSDSHS
                     WGFVPIENLLGRALVIYWPLNEVRLLNGPDIVNAASTP"
     gene            complement(37654..38574)
                     /locus_tag="PKL11_01175"
     CDS             complement(37654..38574)
                     /locus_tag="PKL11_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ12237.1"
                     /translation="MSLLTLSSVLFGAAQTNKAPIEAQAVVVDIWPEYDRTAVLVIYT
                     IRLSPQVKLPATISLSIPRAATTLYQASYYVGSSDSAPTPLNVQTISGADWTQIKLTA
                     PTSLIRLEFYDPRLLINEATRTYTYIWPADFTVDDLTVRIQNPIGATQMEITPHLGGG
                     SLGEDGLIYYETTIGKVDVGMPFNLALTYQKNDSTLTFTTQPVSAASPLDEHIAGRTR
                     LVTILPWLIAGFLLLVALILGVWITWRRMNPEWVEKYRPVWARQLRPKNNLSHDTAVF
                     CPECGKRAEASDLYCRVCGTRLRGRAAETH"
     gene            38780..41302
                     /locus_tag="PKL11_01180"
     CDS             38780..41302
                     /locus_tag="PKL11_01180"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNZ12238.1"
                     /translation="MGDSSRFYLGKKFDLASGKLLDQPVLYDPADLTTHAIITGMTGS
                     GKTGLGIGMLEEAALQRIPAIIIDPKGDLTNLVLHFPDLLPQDFEPWLDPEAARRAGK
                     PLPDLAAETAARWKQGLADWGLGREQLLALQQAAQYSIFTPGSSSGLPVNILSSFRAP
                     EIPWEENSEILREKVSSTVTALLGLVGIDNIDPLRSREHILISNLLEHAWSRGQSLDL
                     TELILQTQTPPFDRLGAFPIDNFYPEKERFELAMLLNNFLATPSFQIWLEGQPLDIGA
                     MLYMPDGRPRHNIFYLAHLSDNERMFFTTLLFAAVEGWMRAQRGTSGLRLMVYFDEIM
                     GYLPPVANPPSRPIMLRMLKQARAFGVGLVLSTQNPVDVDYKALSNAGTWMIGRLQTD
                     QDKQRLLDGLESASGALDRADADKLISGLQKRVFLLHNVHQSAPVTFQTRWALNYLAG
                     PLTRGEIPALNKLAKAVAPTAAPAAAAIPVSPQAASIAETQPTPVAAAAAPKPAAPAP
                     NGSQTRPAAPVGVMEFFLPNDLGISQALAAANLQLAGLIEPEGILYQPALLAQAQVRY
                     TSRQYNVDSARRVAVLLPQADAGLIRWEEYPWGPYEPAALSSQALPKARFEALPAWLT
                     DARRLTALQKDFIEWVYRNGSIRLRSNPTLKLVAGPDVSQAQFREQCSSAAKQAMQAE
                     VDKLETTYRTKLETMKQKIKRQEKEVESQEDEVSQRRTEELGTHGELVLSLLGGRKRS
                     ISSSLTKRRLTQQAKADLEEEKQELDALEEQLIAMEAEHKAALAAIQERWSQAANDQS
                     EVPLSPLKKDIFVEAFGIAWTPYYLIRNGDKLEKLAAFTAPA"
     gene            complement(41368..41976)
                     /gene="recR"
                     /locus_tag="PKL11_01185"
     CDS             complement(41368..41976)
                     /gene="recR"
                     /locus_tag="PKL11_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNZ12239.1"
                     /translation="MPVLPLPVQNLITALERLPGIGPKSASRLAFYLLRAPDEISGQL
                     AEALLKLKSSTALCQICFNISLAEQPICEICASSRRDASVICVVEEPLDVLALERTAG
                     YEGRYHVLHGVISPIEGIGPEDLKIRPLIERIQAGGVREVILATNPSMEGDATALYLR
                     QQLLPLGVQVTRLARGLPVGGDLEYADQNTLLRALSGRSEMG"
     gene            complement(41986..42333)
                     /locus_tag="PKL11_01190"
     CDS             complement(41986..42333)
                     /locus_tag="PKL11_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNZ12240.1"
                     /translation="MAKGFNRPVGGRNQGGGMMQQIQRLQEQMAQAQAQLAEETVTAT
                     VGGGVVKVTMTGAQVCRSVEIDPEVLKDADAEMLQDLILSAVNLALDQSRELAAQRME
                     PLTGGLAGMPGLF"
     gene            complement(42353..44005)
                     /gene="dnaX"
                     /locus_tag="PKL11_01195"
     CDS             complement(42353..44005)
                     /gene="dnaX"
                     /locus_tag="PKL11_01195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNZ12241.1"
                     /translation="MTQALYRKWRPHLWEQVVGQEHVVQTLKNAIRSERVGHAYLFAG
                     PRGTGKTTTARLLAKAVNCLEPDPAQRPCDHCEFCTAVNEGRFLDLIEIDAASNTSVD
                     DVRDLRDKINFSPSQGHYKVYIIDEVHMLSTAAFNALLKTLEEPPAHAIFILATTEVH
                     KIPATVLSRCQRHEFRRIPVSNIVANLQEIASNEGLKADEDALTLIARQATGSMRDAI
                     SLLDQVASTGQHISLDLVQTVLGTATSKSVLDLVGAIRSRQPAAGLDSIHQALDSGTD
                     PRQFARQTIDYLRALLLVSMGNARLVDATDEARAQMAALAQDFTRWQLLEIVRLFNAA
                     ANEARSGWQPGLLLEVALAESIAAMEQAEMRQMSVPQAVAYPEPIITPPLAQSAPPQQ
                     APVTAPPPLPPEPVPEQRSPTPAAEQPAAAQAASTPAAPLTGGELTLQALTQAWPQIR
                     MIVKKTRPPTEALLNSCKNISLREGVLILGFSTEVVKSKMESNDNIRITQDAIAQLLR
                     VRPAIRCVVVGARGGGLSADGEDPDGLVGTALNLGGKIVQKE"
     gene            complement(44022..44537)
                     /locus_tag="PKL11_01200"
     CDS             complement(44022..44537)
                     /locus_tag="PKL11_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNZ12242.1"
                     /translation="MKSWKRVIPYLLLNVLVSALTTILVWLWVVRPQYTALLAELQPS
                     PQPTLALTLLPNPTPLPTATVPPLGEPLVEITSVFGLGDVSTESITLTRIGSGDLWMT
                     GWQLRDEDGHVFTFPEMIWNSGSIQIYTLAGRNTAAGLYWGLTEPVWESGELVLLTDS
                     QGNERAQLRLP"
     gene            44600..45091
                     /locus_tag="PKL11_01205"
     CDS             44600..45091
                     /locus_tag="PKL11_01205"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNZ12243.1"
                     /translation="MGKMPYLIDGHNLIPKVPGLSLGAMDDELSLIDQLQVFCRISRQ
                     QVEVYFDGAPAGQAGTRRYGQIPVHFVRLGRTADDAIAERLARLGRAARNWRVVSSDR
                     RVQAETRARGAEVVTSEVFAAEMRAAQARADEQPGGADRKLSSDEVDDWLDLFNRGNE
                     KPG"
     gene            45344..45417
                     /locus_tag="PKL11_01210"
     tRNA            45344..45417
                     /locus_tag="PKL11_01210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45378..45380,aa:Arg,seq:cct)
BASE COUNT        10184 a        11817 c        14082 g         9135 t
ORIGIN      
        1 cttgactctg acagtgtggt cattaaaata taattaatgt ctgcgttgcg gatatgtttt
       61 gcggggttct ctgggagaaa agcgatgaag acactaagac ggagctgctg cgtaatattt
      121 atgatcctct gggtgttgag cctgattatg cccggtacgg gtgttcaggc caggacgcta
      181 tcaacggtga ccatcagcgg cattatcacg gatggcgggg ggcatggcta tcccctgtac
      241 gcgcgggtga gcctggaaga tggggagaca gcgtatatcg cctatacaga cccattcagc
      301 ggggcgtata gtctgaatgt aaccggagat gtcacttatc agatgcacat tgaagccgtc
      361 atccccggtt atctggcaga agaaaccgaa ataacaccag caacgaatga aactgttaac
      421 gcggcgttgg aagtggatgc gttccgctgc attgcgcagg ggtatcaacg cgtgatatat
      481 ggcgggggaa cgtttgaaaa ttttgacggt gttcccgcac caccgatgat accgggcgaa
      541 tgggggcagg ttgatacctt gggaacagga ggcaactggg cgactgctac aaacacacaa
      601 cagccggctg gttttccacc ttattcctcg ccgaacctgg cctatttcaa ttcttattcg
      661 gctccggata ttcgatcaag attgtacaca ctggcgggaa cagatgcatc caccagtgat
      721 ggggtaatgg tcgaggtcca ggtattccac gataccgggc tgccctataa tgatgatcgc
      781 ttacagatgc aatattcact ggatggcgtc aactgggtga atgtgggtga gcccatccac
      841 cggtacgatg ggtcgaacgg atggaagcag catatggtca cgctgccagc cggggcagcg
      901 gtctacctgg gcatcgaggg gatcagcgag tggggatacg atattcatat tgacagtatt
      961 gattggaacc cgtacacctg tgagccggaa gatcacggcg gtattgtggc ggggtatgtg
     1021 accgacagca ccacgggtga accgctggtg gggcgcgtct tgggcaatga gatcagcaat
     1081 acgctattca ttgacgaaga cccggagcac gccgggttat actggctttt tgatacgatt
     1141 gaccataaag acccggcggt catcacggct tcggcttacc cctggtattt gccagagacg
     1201 gcgaatgttg atgtgattga aaacgggctg gtgcgggttg attttgcact ggaacctgcg
     1261 ccgttacaaa ccatcagcgg ggtggcagcg gatggcagcg ggcacaccta tccactctat
     1321 acccaaatcc atgtgaccgg caacctggac gggctgcccg ggaacgggtt tgagtttgat
     1381 caatgggttt atacagaccc cttcacgggg gaatatcaga ttacggtacc cgaccagcat
     1441 acttatgggt tggaaacgga aacctttttc gatgggtatg atgccgtact ggtcgaagca
     1501 acggtcaatg gcagcccact gactcaagac tttcatctgc aagcttctgc tggctgcacc
     1561 gcgccgggat ataccccctt gaaagcggca caacccgatt tgactgaggt aatggcagac
     1621 tgcgtccccc agtcgggagg gctggtcggg gggtatatca atgatggtaa tacggggctg
     1681 ccggtgatag gggcagcagt aagctcggat ggggtgccgg ggttcaacgt gcagtcggtt
     1741 tggtggggca cagacagcgc acatgccggc gtgtacctgg cttttcagcg caacccgcca
     1801 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatatgtt ggtccacctt
     1921 gatctggcaa ccggcgccag tgtgccaatt gggccgaccg gggtcaacgg caccgaggtt
     1981 cagggattgg attttgacga agcaacgggc acattgtact ggtcagccag cattacaccg
     2041 gcagagagta agctctatac tatttctccc atcaccggcg cagccacact ggtgggatca
     2101 attggcgcca gccaactggt gggctgcctg gcggttgcca gctttaatcc gccaaatgtt
     2161 tcatggttaa gcctggggca aactaccgga acactggcag cgggggaatc gatcagcatc
     2221 ccggtaaatt ttgcgccgga tgtgctgctg cctggaattt atgaagccgg gctgctgctg
     2281 gttgataata caccttatat gcccattctc ggcggtaact ttattcaagg gcgcggacgc
     2341 attcccatta ccatgactgt ggaaccaccg ctatttgaga aaagcgcgag cccggagacc
     2401 acgcatggca gccagctcac atatactctg agctttgatc cgctggcggc aaccggaaca
     2461 atcgcccaaa ttgtggatga gctgcccgcc ggggtcacct atgttgacgg gtcgctcacg
     2521 gtgagcagcg gttatgccag ttataacccg ggcaccaata ccattgtatg ggcttctgtg
     2581 ccacctaaat ctaccaaaga tcagggaaca attgttgaga cactggttac cattacgttt
     2641 gatgttatgg tcacggcacc gcgcggcgaa acgatcagta accgggcaga cttgagctat
     2701 ggcggctatg tggctgaccg aacggttgat accgtagtgg ttaatgcccc gcccagcgct
     2761 gatgatcagg ccttcagtac agatgaagat gtcgattatt ccggtacggt ggctggctct
     2821 gatgctgacg gcgatacact gacttatggg accatcagcg aaccggaaca tggggagata
     2881 accctcaatg ccgagacggg cgccttcacc tactcgccgg atgataacta caatggaagc
     2941 gatggcttta cattcagcgt ggcagatggc tacggcggaa ctgatacggg tacgatcaac
     3001 atcaccatta cccccgtcaa cgatgcgcca atcccacccg cggtttcaga tgtgcagtgg
     3061 gtgatgaatg cagcgcatac ctatgcgatt ccaccgtttg aagacccgga tggcgatgcc
     3121 ttgacttaca ccgccagttt gagcggcggg gggaatctgc ctgagtggtt aagttttgat
     3181 gatcagaccc gcaccttcag cggaacgcct ggtgaggctg atttgggaga ttatccgatt
     3241 gagattaccg ccgccgatcc cgatgtggca actgggacaa caacgttcac gctgaaagtg
     3301 gttgcggagt tgatggtaaa tatctacctg ccggctattc tcagataatg aggtttggta
     3361 tgcccacgca gcccctcccc gcacgagcgg ggaggggcaa cgcgtttagc caaattcatt
     3421 ttctgaaagg cttgagagga taagggcttc atggcggaga gccggtgaaa gttctgataa
     3481 aatattgata tatcgccagc acgacattaa tacacggcgt gctataatcg ccttactggc
     3541 ctgattcggc ccgaaaagga acccacacct atgatgagat ttgaccgctt tactgaacgc
     3601 gcccaggaag ctgcccagcg tgcggctgag attatccagc gctatgggca taatcaaatt
     3661 gatactgagc atatcctgct ggcgctgatt gaacaaccgg agggggttgt accgcaaatt
     3721 ctggaaattc tcaaagttga ccccacccaa ctggtggaac ggctggatta tattctgcgc
     3781 accagcccca aggcaagcat ttttggcgcc ggagccgggc agatcttcat cacgccgcgg
     3841 gtcaagcgga ttattgacct ggcgaacgaa gaagccagcc ggttgaagga tgagtatatc
     3901 tcgacagagc atatctttct ggcgatttta tcggaacgca atacgcccgc agcccggctg
     3961 ctggaaagcg ccgggattac gcgcgagcgg gtgaacgagg ccattggcca actgcgcggc
     4021 gggcagcggg tgaccgaccc gcaggctgag acccgctacc gcgtcctgga aaagtactcg
     4081 cgtgacctga cccaactggc gcgcgagggc aagctggacc cggtgattgg gcgcgaccag
     4141 gagatccggc gggtgatgca aatcctgtca cggcgcacca aaaacaaccc gattctgatc
     4201 ggcgaggccg gcgtgggcaa gactgcgatt gtggaaggcc tggcgcaaaa aatagcctct
     4261 aacgatgtgc cggaaatttt gatgggacgg cgcgtgattg cgctggacct gggcgcgatg
     4321 attgcgggat ctcggtttag aggtgagttt gaggagcgcc tgaaggcggc gattgaagag
     4381 gtgcagcggt cggaaggtga gatcattctg ttcattgatg agctgcacac cgtcgtaggg
     4441 gctggggcgg cccagggcgc catggacgcg tccaacatgc tcaaaccggc gctggcgcgc
     4501 ggtgagctgc agtgcgtggg ggcaaccacg ttagatgagt ttcacaagta cattgaaaaa
     4561 gactcggcgc ttgaacgccg ttttgcgccc gtctatgtgg acgagccaac tgttgacgac
     4621 accatcaaaa tgctgtttgg gctgcgcgac cggtatgagg cgcatcacaa ggtgcatttt
     4681 gcggatgatg cgctggtatc tgcggcccgg ttgtcttcac aatacgtcac tgaccggcgt
     4741 ctgccggaca aggcgattga tctgatggac gaagccgccg ccaagctgcg cgtggcattg
     4801 tacctgatgc cgcctgacct gaaacagaag aaaaccgagc ttgaccggct gcgcgctgaa
     4861 gaagagcagg ctggcttgga gcgcgattat gaacgagccg cgcgtaagaa agccgaacgc
     4921 atccgcctgg agacggagtt taacgaagaa cgcgaccgct gggaagaaga acacaagctg
     4981 gatgaagtgg tggatgtgaa cgatattgcc gaagtggtgg cacagtggac gggcatcccg
     5041 atcagtcaga tgatggaaaa cgaagccgaa cggctgctgc acatggaaga ccggctgcac
     5101 gagcggatca ttggtcagga agatgccatc catgccattt cggatgccat ccggcgagcg
     5161 cgttcggggt tgaaagaccc gcagcgccca attggatcat tcatcttcat tgggccatcg
     5221 ggcgtgggca agaccgaact ggcaaaagca ctggcagagt tcatgttcgg tgatgccgaa
     5281 gcactggtgc ggctggatat gagcgaatat cgcgagcagc ataccgtctc gcgcctgttt
     5341 ggggcgcccc ccggctatgt gggctatgaa gaaggcggcc aactgaccga ggctgtccgg
     5401 cgccggcctt accgcgtgat tctgtttgat gaaattgaga aggctcaccc ggaagtctgg
     5461 aatgcgctgc tgcaaattct ggatgacggc cgcctgaccg atggacaggg caacgtggtg
     5521 gacttccgca atacggtgct gatcatgacc agcaacctgg gcaccgaatt tgtgcagcgc
     5581 ggcggcacgc tgggcttctt gaccgccgac acgaccggtg aagagcgaca gacgcatgat
     5641 aagattgaaa aggcgctgaa gagtaccttc cggcctgagt tcctcaaccg gattgatgag
     5701 atcatcatgt ttgcgccgct ctcactggat gagatgcgac tgattgtgga cttgcagatg
     5761 aaggaagtga gcacacggct ggaagagcat ggactgcatg tggcattaag cccggcggcc
     5821 cgggagtggc tggcgcaggt gggatatgac ccggcttttg gcgcccggcc actgcggcga
     5881 gcgctgcaaa agcatgtgga gagcccatta tccgtcagcc tgttgagcgg acagtttgcc
     5941 cagggtgata cggtactggt ggacctggat accgagaaga acgccctgac gttcctcaag
     6001 gcagaccagg cttaaagatc cccaagatca gctctacggg cgggtgattc tctcacccgc
     6061 ccgctttttt gggtgtggta caattcgggc gtatccacaa caggaggatc atgatgaaaa
     6121 aagcgttgat tactggcatc accgggcagg atggttcgta cctggccgag ctgctgctct
     6181 cgaaaggtta tgaagttcac ggcatcatcc ggcgggccag caccttcaac acccggcggg
     6241 tggatcatct gtatcacgac ccccatgcca atggagaaga tgtcaaactg tatctgcatt
     6301 acggcgatgt atcggtgggt gaaacgctgc tcaatgtgat ttacaatgtg cgcccggatg
     6361 aaatctatca tctggcggca caaagccatg tgcgggtgag ctttgacatg cccgaatata
     6421 ccggggacat caccggactg ggaacaatcc gtattctgga tgccattcgc aagagcggca
     6481 ccgaggcgcg gttctaccag gcctcatcca gcgagatgtt tggcggcgcc aaaccgccgc
     6541 aaaacgaaga gacacccttt gagccacgca gcccctatgc ggcagccaaa gtatacgctt
     6601 actgggtgac gcgcaattac cgcgagggct ataagatgtt tgcctgcaac gggattttgt
     6661 tcaatcacga gtcgccccgc cgcggcgaaa cgtttgtaac ccgcaagatc acacgggcgc
     6721 tggcggccat tcgggcgggg aagcaaaagc acctgtacct gggtaacctg gattcacgcc
     6781 gggattgggg ctatgcgccg gagtacgttg aggtgatgtg gaggatgctg cagcaagatc
     6841 agcccaagga ttatgtcatc ggtacgggcg agtcccactc aatccgagag tttttggatg
     6901 aggcctttgg ctatgcgggg atggactggc atcagtttgt ggaaattgac ccgcgttact
     6961 tccgcccaac cgaggtggat tacctgcttt cagacccgag ccggtcacgt aatgagcttg
     7021 gctgggagcc caaagtgcgc ttccatgaac tggtgcggat tatggtagat gctgacctgg
     7081 aactgcaagg gctgccctgc cccggcgagg gtcgcaaaat cattgaggat cggtttggcg
     7141 gctggcaccg atgggaacat caggtggtca gcatggagcg gtaaggcgcc gaccgaaaag
     7201 gttataagaa accccattca gagcttctgg atggggttct tcttgatttg gggacaataa
     7261 agaaatcagc gttaattcaa gggggttatc gatcagccgg ggcgtaaacg atgaggataa
     7321 attcagcttt gcgggcgccg acctgctggc gcacctcggt gatggagccg cgcaggatcg
     7381 cctcggaggg gagagtcaga tccatggcaa gcaagatgga atgattggcg ccaaaggcgc
     7441 gagaaacctc ttccagcagg gcgcccaggc ggtagggcgt atccataagg acgatgggca
     7501 tgcgcaggct gcgcaggcgc tgcagttcct ggcggcgctg atccggttgg cgggccagaa
     7561 agccgccaaa gacaaaccgc tccagcttga aatccagcag ggagagggca gtcatcaagg
     7621 atgagacgcc cggtatcggc tggacacgaa attcaagttc ggcagttcgg cgcaccaggg
     7681 catgaccagg gtcggcaaat acgggcgtgc cgcagtctga aatcagggca aaggactctc
     7741 ccatggccag gcggttgaca atttcatcca cacgctcggc ttcgttatgc tcattgaggg
     7801 taagcaactg gcgcggggta atctcaagac gcttgagcaa ggtggttgcc gggcgaagtt
     7861 cctcacagat gattgcgtct accgaggcaa gcacctccaa agcccgctga gtgatatcgc
     7921 cagggttgcc aatgggtgtg gcaacaatgt agagggtgcc gggtggtttc atcatgcgtg
     7981 cctccgcagt acctgccaca acagaccacg ctgcggggcc aggaagaaag ccagcaaaaa
     8041 gaatagggtt gccgacagca caatggctgc acccgaagcg atatttagat agtaactgac
     8101 atacaggccc acaatgccag agaaagcccc gatgccagcc gagagcagca tcatcacccg
     8161 caggcggcgg gtgagcaggt aggcggtggc agccggagta accagcatgg cggcaaccag
     8221 gccaatgccg actgtttgca tggaaatgac aatagtcaaa gccagcaaaa ccagcagcag
     8281 attgcgcagc aaacccgtcc gccagcgcag ggttgtggca aggacagggt caaaagccat
     8341 gaccaggaag gggcggtaga aaaaagccac caggccaatg accactacgc ccaggccagc
     8401 cgtcagccat aaatcggcgg gggtgacccc cagcacatcg ccaaaaagga tgtggctgag
     8461 gtccactgcg taggtgcgga tgctgctgat cagggctaca cccaatgcca gcgccgcgga
     8521 gaagaggatg ccgatggccg tatcttcttt caggtcaccg cggcgggaga ggaaaccgat
     8581 ggaaagcgcc accaggaccg ctgccgccag tgcaccgatg agcaggttgc cgccgatcag
     8641 atacgccaca gcaacaccgg gcaaaatggc atgtgccagg gcatcgccga ggaaggccat
     8701 ggagcgcaag acaacataac agccgattac ggcgcagatg atgcctacca aaatggctgc
     8761 cagcaaaccg cgctgcatga aggcataggt caggggttgg agcaaggcat caagcatggg
     8821 tatgctcctc gtcgtgctcg tgcgggcagc attgatcaac aacaatttca gcactaccgc
     8881 ccaggctgag caactgaccg ccaaaagcct cggcaacatg ctgatgggtg aaaacctgct
     8941 cggacgggcc gtaggcgatc agacggtggt tgagcagcaa agtgcgttca aaccgttcgg
     9001 ctgccagctt gaggtcgtgg gtggagacca gggtggtcac accagactga cgcagatcat
     9061 ccagcaggtt cagggtggcc tcctgagtgg cagagtctac gccggtgaag ggttcatcca
     9121 tcagcagaat gtgcggctcc tgagccaggg cacgcgccag aaaagcccgc tgctgctgcc
     9181 caccggagag ttcaccaatg gggctgcggg caatagcctt gatgttcaac tgctccaggg
     9241 cgcgcagcac cgcggcacgg tcactggcag aaggacggcg cagccagccc gcccgtccat
     9301 agcgccccat catcaccacg tcccaaacgg tgacggggaa gcgccagtcc acctcttcac
     9361 gctggggcaa atacgccacg caatcatgat gatgacccag cggctgacca tgaatccgga
     9421 tttggccggc gcgtaccggt aaaaggccca ccagcacctt gaaaagtgtt gatttgccag
     9481 cgccattggg ccccacaacg gcaacctggg cgccatgcgg gacatcaaag gtgatgtctt
     9541 gcagggctgc ccggccatta tagccaacgg tcacatggtc taactctaac ctggcaggat
     9601 tatcggccag gggttgtttt tgagactcag acaagcacaa acctcttatc gaaaatgatt
     9661 ttcaattata gccagcgggc aggcatttgt caatcaagac caggacgagt acaattatcg
     9721 tatgatgtct gcgccgaccc agttggatca cctgcgagag gtggatgccc gccgcgattt
     9781 gaacgcgatt gcgaacttaa ttgatatttg cttctgggcc cacctggacc cggaaggacg
     9841 ggcttatttg aaccggctgc ggcggctggc agttcagcta caggtggttc cgctgcggag
     9901 catggcaaat gcggcagtgc caattcaggg gtttgtgtgg gaagaaaaag gcgaggttgt
     9961 tggcaacctg acgttgattg cttttcgcag ccgggggcag ctgctgtatc tgattgccaa
    10021 tgtggcggtg caccccgatt accgacagaa agggattggg cgggcgctga ctcagcgcgc
    10081 gctggattac atccgcgcga cgggcgctgc cagcgcctgg ctgcatgtgc gggcagacaa
    10141 tccgacagcc cgacacctgt acgacacctt gaagtttgaa gaacgctgcc agcgggtaaa
    10201 ctgggtctgg cggcctgagg atcaggttcc ggtggtcaaa ccgccagcag gaatagcggt
    10261 aggaacacgc cgaaaagccg aatgggggca gcagcgagcc tggctgcaga gcaattaccc
    10321 ggccgcggtg gactggaatt tgatgtttga cgctcagcgg ctgaagcctg gcgaactgcg
    10381 aagcctgtgg cggtggttga acagcgccaa tcagacgcat ctgactgtgc ggcgggatga
    10441 ccagccgctg gggctgctca cctgggagac cggcgccggg cagcacagct ttttctggct
    10501 ggcggcgcca gatcagcccg acgaagccgc actgacctgg ctgctgatgc aggcacgcca
    10561 cgaatggacc acccgggcgc ggttttataa tgcggctctg cggcctatgc aaatcaacta
    10621 tgctgccggg caggctgcgg cagcctttga agcggcgggg ttttcggcag aaaatcatct
    10681 ggtatggatg gaacatcccc tgcggtgaac gcctgcaacc tttgacctaa aaagtcgtat
    10741 atcttagcgg aggaacagat atgaaaaagt ttttgattcg tctggcggtt aatgcggtgg
    10801 cactgtatgc tgccgtggcc ttgttgaaag atacctatat ttacccacaa agcgaaaact
    10861 ggaccagcat tatctggctg gcgctgattt ttggcctggt gaatgcgatt ttgcgtccaa
    10921 ttgtcataac ggtgggctgc ccggtgatca tcctgacgct ggggctgggt acactgctgg
    10981 tcaacacgct gatgttctgg ctggcaggtc aaattgggct gcgctttgac gttggctttg
    11041 aggtggaagg cttctggggc gctttcctgg gggcgctgat tgtcagcgtg gtcagtttta
    11101 tcctcagctt gatcttccgc gacgcgcgca agagccggga ttaatcctac caaaacgaaa
    11161 accgggcggt tcagcagacc gcccggtttt tattttctct caaaccgatt tatcagggca
    11221 gggtaaagcg caggatcaga ttgttggctg agtcggacac ccaggcgtga ccctgagcat
    11281 ccactaccac agcactgggg ttggcaaagc catctgacag ggtgctgtag ccgccccagc
    11341 cgccgacaaa ttcgccggtg tcggtaaatt cgagcacgcg gtaattaggc ggatcggtca
    11401 caaagatatg gccccggtta tcaatcgcga ggaagggttt gttctccaca tcctggcctt
    11461 tccagccaga gatatcgaat gcgctgaggg gggtgaaggc ggtgtgattc tcatttggga
    11521 ggaagatttg cacccggcgg ttccaggtat cagccaccat gacgcggcca tcaaccgtta
    11581 cagcgatgcc gacaggctcg tcaaactcgc ccagcgagag gccggcggtg ccaaactcgg
    11641 tgatggtttg accttcagaa gtgaagacca ccacgcgctt gttgcccgta tcggtgacat
    11701 agacgcggcc atcagaatcg acagcaatgc cgcgcgggcc atagaagacc gggccacccg
    11761 cagcagggtc ggtggtgccc caggcggtca aaaacgtgcc gtcggcagtg aatttttgga
    11821 tgcggtaatt ccaggtatcg gtgacataaa ccgagccatc gggaccaact gccacgcccc
    11881 agggctcatt gaagtagccg ccagccggtt gaacgccagc ctccaccgcg gcaaactggc
    11941 cccaggaatt gagcaaagtt tcgccatcgg cgctgaaatg cagaatccga tgcgcccggg
    12001 agtcggcgac atagagtgag ccatcggggg ccacggccag gccgtgcggg ttggtcagtt
    12061 ggaccgggtc tccgccaacg ccgaccacaa tatccgggct gagcacctgg atcttctcct
    12121 gataggggtc aacctcgggc tcgggagcgg cggcggcagc cacgccataa ttccagatct
    12181 gagcagcgat atcgcggcgg acatagagcc gcatgcgcgc cgagggctgc cagttttcca
    12241 gggtcaggtt ggtcttaccg gtcgtttcag cgtagagatc atagtcgcgg ttcagccaga
    12301 tgttatacag cccggtgcga agctgcgggc tcttgagggc gttgccaatg cgctgccagg
    12361 tgaggccgta gtagtcctgc atgggccacc agaggcgcat gtaatcaaag ccgatgaact
    12421 gatctttgac aattggttcc aggcgggaga agttggcttc gcctaccaca atcatatcgg
    12481 cggtttcgag gtcgcgggtg gggtcggtaa accagcgctt gttgggatag tcgcggaagt
    12541 accaccagcc gccatacaag acatcgttgt cataggcgac tatggcatcc cgcccgccgg
    12601 tggtgcgccg cgagatctct tcgacctgct cgacaacgtc tttgaaatca ccggaggcat
    12661 gggcatagac caagaactct ttggcgttat cgtagttaat gaaggaagcc cgataggcat
    12721 ggcgggttgt aagcactgcc agaaagacaa aaacgaccag agcagtcagg cgcaggattt
    12781 caacagtgcg ccatttgcgc aacaggttga aggtgcccca ggcgcaaaag acggtgacga
    12841 tggaccagaa gacaaaggcg taggttgccg aaagctgttc ggtggtcagt gtgtttccgg
    12901 tgaggggttt ttggaagggt gggttaagcc ccagaacgct gccaagcaga ttggcaaccc
    12961 ccagaaggaa ggcggcgatc atgaagacag aggccagacc ttctttgtta cgcagcgatt
    13021 gccagtcaat tttgtctgcc agccagccca ccgaccagcc cgaagccagg agcatgggca
    13081 gggtaatgtg aacggtgagc cagggcatct tttctccggc caccgagtag gcaaccaggg
    13141 cggtcagaga ccagaagagc aagagggcca gggtgggcac ttttgctggc cgggcgacgg
    13201 gttcaggcgc cggggttgga tcattggcct cggacgcaat tggtgcaggc aggggttgtg
    13261 gctgtggcgg ctcggcagtt ggcgagtcgc cggggagggc agacaggcgg ttgtggcgga
    13321 agcccaggta ggccgccagc agggtgccca gggcagccag atactcatac atggggattt
    13381 gcaggaaggc atagtaatac cagggttgag aaccgcgctg ctcaccctgc tgactgagcc
    13441 agtaacccag cgagccgaca atgccggaga agaaaccgta tccgttggtg aagaaggtgg
    13501 tgtataagac tgcaaaaaca gcatagaaca gcacggcatt gccaatccat aatggtgcgt
    13561 tccaccacat gccaatcagg ccggagataa ccagtaagat gaccagtgtg acaatgatgg
    13621 tgataatgcc agccgtggag gtataatcca gcgggttgcc gcccagcatt ttgatgggga
    13681 aggcggtcaa ctgcggcagg accagcgtgc cgaccagaat gagtaagtca aaagaacgtt
    13741 ccgcacggat tgctttcaaa ccaagggagc gcaccagcgt caccagggca atgacgcccg
    13801 agataattgc cagcccgacg caaaagagcg ttgccgtgag gatggtatta atcaattggg
    13861 gggagacgga cgcgggatcg agagcggctt gcttggttaa atcggcctca taggtaccca
    13921 ggccaacgcc gatgataatc agtgataatg cgacggccat cagcacgatg aacagcgtcc
    13981 ggcgccgatc ctcgggccag cgggtctgag cgatgcgtac cagaaagagc agcgccagga
    14041 agagcagcaa ctgggcggcg taaataaagg aggtttcttt agtgcaaaaa tgcaggacca
    14101 gggcagccgt gaggatataa agcgaggatg gtttaccgtt atccagatag cgcaacacag
    14161 caaagagcat catcacgccg aggagctcaa taaagccctc gttgcgggtg tagcgaccat
    14221 agaataacat gtagggcgaa atcaagaaga agatgccggc aaacagcgct cccttgcgcc
    14281 ctagatagcg gcggaagccg aacaagacga aggccaccgc cgcggtgctg aacaaagccg
    14341 ccgggatgcg ggctgagaag tcgttgtcac cgaacagggt atagctgagc gccaggaggt
    14401 ggaactgcat ggggccatgg gtgatggggt cgtgacgata gccgcggcct tcgtagaggt
    14461 caaaggcagg gaccacgtga ttgacttcat catggctcat ggcgcgccag ccaacgccat
    14521 ataaacgcga aaccagggct atcaggatga tgagtaccgc cagcaagttc tcaacggtca
    14581 accagcgggc ataggcagtc aggggacgat cgagccagga ttcggatttc tcactcatag
    14641 gtgtcttttc cagaggtgat gatgggttga ttgccgcaag gtttttgacc agggcaacat
    14701 gggttgatta tactcgaatt gaggcagggt ttcagattgt aatgatacaa tacatctata
    14761 cccccaatcc aggatgctcc catgatgcct tccaagccag cgcgttacag cctgtttgca
    14821 ctgctctatt tttcgcaggg tgcggtgttg agctttttca cggcgtttaa tgccatttat
    14881 ttgctctcgc atgatgtgag catgacccgg attggtttga tgggcacaat tgcactgctg
    14941 ccctttattt ttaagatttt cctgggcatg ctcagtgacc gggttaattt gctggggcac
    15001 gggcaccgca agccgtatat tttgattggg ctggtggtgc aggcagtgtg tctgctggtg
    15061 gtgccgcagg tgaacccggg cacacacttc tggctgtatg tggccctggc ctttttgctg
    15121 caactgggca tggcgcttta cgacacctgc accgatggtc tggcgctgga taccaccccg
    15181 ccggaagaag aagggaccat tcaagggttt atggtgggtg ggcgagcgct gggggtggtg
    15241 ctgattgccg ggatcattgg gctggtggca gaccgctttt cgtgggcggt ggcgttttac
    15301 acgctggcgg cgctgtctct gctgcccatt ccgctgctgc tgaaggcgcg cgagatcaaa
    15361 cagtctgaag ggcggcggtt tgaatgggga gcattcaagg cttttgcaca accagcggtc
    15421 attggactgg gactgctggg ggcgctgtat tcgctcatca tcaacggcgc taatgaaatc
    15481 gtcaatccgt ttttgcaaag caggttcagc atctcgacca cacaggcagg gtttttgacc
    15541 gctgtctggg ggttgggcgt ggttctgggg ggcatcacgg gtgggcggct gaccgaccgg
    15601 ctgggacatg cccgcgccac gcaggtggcg atgatcatgg cgctggcagc aattgtgccg
    15661 ctggcgttta ttcccagcgc ctggctggcc tgggggctgg tggcggtctt tggactggct
    15721 tacgggttct acgagaccgt ttactttgcc atttcgatga attacaccga ccggcgcatc
    15781 gccgcatcga tgtttgcgct gctgatggcg gtggccaata tcggcacagg cattgggctg
    15841 gggatcagcg gcgcgctggt ggatagcctg gggtatccaa tcacttttat cattctggca
    15901 gggctgaacc tgctggcaat ccccttatta accatgattt ttggacgggc aactgaaaag
    15961 gtacaggagt attaatacat gcctacaaat ttaccggaaa tgccatttcc caattcttac
    16021 tgggttgagc ctgggcgatt tatggcggga gaataccccg gcgtgtatga agtcagccat
    16081 tcaaaattaa gattggagcg gcttttgaaa gccggagtat cggtattttt tgatttgacg
    16141 caggagaacg atgcccgccc ctatttgccg ctgctcaatg agaccgcggc cctgctgggg
    16201 agaaaaattc actatatccg catgccgatt gtggactttt tcatcccgac ggtggaagag
    16261 atgaagatta tgctgaatca gattgatgcg gcgctggctg cccggcagac catttatgtt
    16321 cactgcattg gcggactggg gcgcaccggc acggtggtgg gatgttacct ggtgcggcat
    16381 gggcaaaatg gcgagcaggc cctgctgtca attcagggcc tgcgggaaca tgtaccgaat
    16441 tggtgggaga gttcgcctga gtctcccgct cagcgagaga tggtgcgaaa ctggaaaccg
    16501 gggcagtaag ccgcctgtta tttattgtct tccagcattt tcagcagcaa accttcaaaa
    16561 tcatcgcact gtgaaaccag ggcgcgaact actttggtga agtattcggc ttccaaaagc
    16621 tgcatttcca cgttctgcat cacagaatag acgtgcttgc cgccaatctc ctcaatcgtc
    16681 cagaagccaa ttttttgcgt ggcgctcttc ttgagcagga tggtcgagag aaaatgcggg
    16741 attttgtctt cggaatcgaa ggccgccagg ctggggactg aaaattcgag ggtgttatca
    16801 tacttgatga tgtacagcgt ctggacgcga cccgattcca tgttgaagcg gatggtcgca
    16861 tgcttatcgt caatttcggc aatcttccag tcttgctgct tgcaatactt ttctaccagg
    16921 cttttgaaac ccggtgagcc aaatagattg ctcatataaa catcctttct ggttgaatgg
    16981 gagatgaaga ggatacggca aattctgaaa ttagtatatg acaatgggtt gaaaaaagca
    17041 atatcaacac aaaaaagaga ggcagtaaaa atcgcggcga gccctggtaa gcgttaacag
    17101 ataggccgat ttatacccgg gaaggtcttt ctatggtaaa atctgtccgc ctgaggcgtt
    17161 gtacccttcg ccttggagta ttttgtgccg aaagggcttg agaagatgaa agttctattg
    17221 attaaggacg tttacaagtt gggtcgagct ggtgatgtgc gccgcgtggc tgatggctac
    17281 ggacgcaact tcctgctgcc gcagggtttc gccattctgg caacccaggg cgcgctgaaa
    17341 caggccgaga ccatccgccg caaggccgaa gctcgccgca ttgtactgaa caatgagatg
    17401 agcgggatcg ccaatctggt cagcgggctg gtgctgaatt tcaccagcaa agccggtgaa
    17461 acgggcaaac tgtatggctc cattaccacc cagatgattg ccgatgccat caatgaaaag
    17521 ctggagacca agattgaccg ccgccagatc gaatgccagc ccatccgcac gctgggcgaa
    17581 cacaaggcgc acatccgcct gacgattgat ctgatcccgg aagtgaagat tgtggtgcag
    17641 cgcgaaggtg agcgcaaggc cgccccggct gtcaaaccgc agcctgaggt tgttgaaacc
    17701 acacccgctg aagttccggc cgaacctgcc gctgaagaag tagaagaaga agcggcggag
    17761 taatccccca ccccaaacgg gatttcaaat gaccggccgg gggcagcctg ttggctggcc
    17821 tggccggtct tgtttttgag gacccatgtc ccccaccatt ggagcgcagt taaaacaagc
    17881 ccgcgaagcc cgcggcctga ctctggagca ggcagctcac gccacgcgcc tgaggctgca
    17941 ttacctgcgc gctcttgaag aagatcagcg tgaggtacta ccctcagaag tacaggggcg
    18001 gggatttttg cgcctgtatg ccgatttttt gggtctgcca gtccagccgc tgctggatgc
    18061 ctggaagacc aaccaggttg ttatcgaagc cctgccaccg gcaaaggttg aagctccgac
    18121 cgtaaccgag ccggtaaaag aagaatccgt ggctccagag gttgtgattg cgccagagcc
    18181 cccgccacca gcggtcatca acccgccgac gaccccgcga gccgagatca ttgcgcccac
    18241 ggcagaatcg cggcagattt tacagcaaat tggggccgag ttaaagcagc agcgcgaagg
    18301 attgagcctg tcgctggcag atattgaaaa acatattcac gtacgacagc actatctgaa
    18361 ggcgctggaa gaggggcggt ttgaagttgt gccttcgccg gttcaggcgc gagggatgct
    18421 gagcaattat gccagcttct tgaatctgga tactgaaaat atgctgctcc gttttgccga
    18481 ggcgcttcaa acccggcgga cagagaacct gccagccgcc ccgcaggggc gcggcgcggc
    18541 caaaccccgc gcagcaaaag cacccggttg gactcgattt ttgacccccg atctgctgat
    18601 tggtggaaca ctggtgattg tgctggtggt ggtgatcatc tggagcacga tgaatatttc
    18661 tgccattggc accacgccag ctacccaaac agccccctca atttctgatg tactgctgac
    18721 cacaacaccg ctggcgctgc cgaccagcga cctgccagtg acagaatcaa ccggcatttc
    18781 tcctgagatg cccggggatg ccaccgttcc aactttgact ctggcgccgt tgaatacaga
    18841 cccaatgcag attgtggtga ttgggctgca gcgcacctat ctaaaagtta cagttgacgg
    18901 ggcggaagtc ttcagcgggc gggtggtgcc ggggaatgca tataattttt cgggcagtga
    18961 gcaaattgaa ctgatcgccg ggaatgccgc cggagtgcaa attttctaca atcaaagcga
    19021 cctgggaacg ctgggggcaa ttgggcaggt ggtcagtttt atcttcacac cgggcgaagt
    19081 catcacccct acgccgagct ttacagctac ctcgccagcc acgcccaccc ccaccccaac
    19141 ccccacgctg acgccaaccc ccggcgccac gccaacggta acgcaatttg tgccataaat
    19201 catgaaagca aacacttttt atctggtatc actgggctgc gccaaaaaca cggttgattc
    19261 aacttcgatg gcgcaattaa tggagagcgg cggctaccgg gcggttgaaa agccggggca
    19321 agccaatatt ttgattgtca acacctgcgg tttcattcaa ccagcgcggg aagaatcgct
    19381 gcaagaatta cgcacactgg caaaacgcaa aaagcccgga caaattttga ttgctgcagg
    19441 ctgcctggca gaacggcacc gcgagcagct tgcccgcgaa gtgccagggc tggatgggat
    19501 tctctcaacc cggcgctgga tggacgtgct caacatggta cagcatctcc gcagcgaggg
    19561 gcacccccag cagccttatg atttacccga agcgcccgat gtggggcggg aagatcacgg
    19621 tgtgctgcgc tcggcgctgc aaggcagcag tgcatacctc aaaattgcgg atggctgccg
    19681 ccgcccctgc gcgttttgct ccatcccatt aattaaaggg ccggctgttt cacgccgccc
    19741 cgagcagatc attgctgagg cgcaaacact ggccgacatg ggcgcccagg agctgatcct
    19801 catcgcccag gatacaacgg attacggcag tgatctgggg ctcaaagatg ggctggcggc
    19861 gctgctggaa gaattgaccc gggctgtgcc gggagtgccg tggattcggg tcttgtatgc
    19921 ttatccgggc tatgtgactg accgtctgat tgaagtgatg gccgggcacc cgcagatttt
    19981 gccttacctg gacatgccac tacaacatgc gcaccccgac acactgctgc gaatgcgccg
    20041 cccggctaac atggaatggg ttcaccgaac gctggagaag atgcgcaagg cgctgcctgc
    20101 gctggcacta cggaccactt tcattgtcgg gtatcccggc gaaactgagg cagaatttca
    20161 gacgctgctg gattttgtga acgaaatcca gtttgaccgg gtgggggtat tcccattctc
    20221 gtttgagccg ggcacaacca gcgaaccgct gggcgacccc attccaaccg aggtaaaaga
    20281 ggagcggatt gagcgcctga tgacgctgca gagcggcatt tcactggagc gtaaccagac
    20341 ctgggtgggg aagacactgc ccgtgctgac agaaggctcc aacgatggca tcaccgtggg
    20401 acgatcatac cgggatgcgc cggaaattga tgggctggtt tttgtggacg gggaactgcc
    20461 cattgggcaa attcaaacgg tgcgcattac cggcgctttg acctacgatt tgaccggcga
    20521 accgatctaa aattatccgc atgtctcgca ctgactggct ggcgctgctg ctggcgatcc
    20581 tgactttttt aggtgcggcc tggattacag accaggtatt tgaaggtctg ccgcacctgg
    20641 aagacgagat ggcttacgtc tggcaggccc aggcgattga aggcggcaaa ctgctgctac
    20701 ccacaccgcc ctgcccgcgc tgttttctgc agccctttgt ggtggattac ggcgggatgc
    20761 gcacggggaa atatccgcca gggtggccca cgctgctggc tgtggcactg cgcctggggt
    20821 tacaggatcg gatcaacccg ctgctggcag ggctggcaat ctggctggtt taccggttgg
    20881 ggaaaaagat caccgatgaa cgcaccggga tactggcggc ttttttaacc gcaacatcgc
    20941 cgttgttttt gatgaacagc ggcgcattac tttcgcaccc ctggacgctg gtgctcagcg
    21001 ctactgtggc gctgggctgg ctggacagct ttgtaatccc caataaacta ccccgggccg
    21061 tgccgctgac tgccgcagcg ctggcattgg gggcgctggc gctcacccgc ccgctaacgg
    21121 cggtgggtgt ggcgctgccg tttatcattc atgccctggt attattcatt cgagggccgc
    21181 gcgaggtgcg ctggcggctg gtggggtttg ctgcgctggc tggaagcctg gcggggctgc
    21241 acttcttgtg gcaggcggct gttacgggca atccactgac caacccttac acgctctggt
    21301 ggccctatga tcagattggg tttgggcctg atgtaggttt gcaggagggg gggtatcagc
    21361 cgatccatgc gtggtacacc accagttaca acctgtgggt ggcggcgcgg gatatgctgg
    21421 gctgggggac ggtctcctgg ttgatgctgc catttggcct gctggcgctg cgccgcaata
    21481 aaaatgccct gctggtaggg agtgttctac cggcgctggt gctgacttat gctttctact
    21541 ggattgcggc ctgggtgctg ggaccacggt attattttga agggctttac agttggacca
    21601 tttttaccgc tgcaggaatt cgctggctgg cggggcagcc gtttgccaaa acagcatccc
    21661 ggtggaaaca atggggacag aaagcacgct atgcagccgt tacggcgctg accatctgcc
    21721 tgattgccgc taacctgatt tactacacgc cgcagcgcct gggcgcgctg caggggctgt
    21781 atagtgtatc agcaacgcac ctggacccct tcagggcggc tgaaaccaag gggatgaccc
    21841 cggcactgat catggtgcat ccggtggact catggataga atatgggcgg ttgctatctt
    21901 taagcagccc atacctcaat acgccgttta ttttcacggt ctcacgcggg ccggagaagg
    21961 atgcccaggt ggcagcgcta tacccggacc ggaaggtgta tcactactat gccgacacgc
    22021 cgtgggtgtt ttacgaggcg ccgcgttagg gtgtgggggt cacaccaggg atttctgttg
    22081 gtacctcagt cgggacttcg gttggcgggg tttcggtggg ggttgaatcg ggcggtgggg
    22141 tttcagcggg agggctatcc tcaccaccgt cttcttcgcc ttcaccgcgt tcacagcgat
    22201 ctaccacgtc ttgcagggta ggctgcagca cgggatcggg cgcgagggtc agggcttctt
    22261 cgtattgttt ttgcgcttca caataatcac ctgcggcggc aagctgatcg ccataaccaa
    22321 tcagcgccag gcggtaacgc tcaccggcgg tatagccgga agaatcgcgc aggttgggga
    22381 cgtaggggac aatgtcggag aagtaattga tgacccgaac ccagtccacc cgccagaaac
    22441 tggctcccgc catgtaatag cgggcccagt tgcggtagcc cagggcttct tcatcaagct
    22501 gggagaactg ttctgacagg gtcagatcat aaacgccaag ctcaaggtta ccttcatata
    22561 aaatacgttg ggcgccgcgg aagcgcagac agatatagta gaggccatcc acatctacag
    22621 tacggtagtt caggttggtg tcccgaagct ggtcaagggt tgtcagggcg gcatcccact
    22681 cctgggcagc catgtggtta agcaccgatt gatacaggtc aacttcgccg cgcaggtcgg
    22741 gcgtgggggt gaactgcggt gtgggtgtta gaatgggggc cggagtcggg gttttaagct
    22801 gggcctgggc aagcatcaca tccatcagct tttgctgggc gtccgggaag gtcgggtcag
    22861 tttcgatgac atactccagg cgctgacggg cggcatcaag acggccggat tcaatgtctt
    22921 gcatggccag accatattga atgctggcca acatgactcg ctgttcttgt tcggccgcga
    22981 ggcggcgggc gatgccagcc cggtatccca cccaggccga cagggcagcc gcgagcagga
    23041 cgatcagtaa gcccagccag aaccagcggg accgacgggg tttggcgggt ggctcagcgg
    23101 tgggtggttc aatctccgct ggcggggggg ccggcggttc ttcggtgatc tcaggtttga
    23161 cgggcagtgt ctccaaaggg ctggaagcga gcggggtagg ccgggtgacg gcctggctct
    23221 ggttcagccg ggaaggcacg gtttcagcag gatctttatc agacatgggg attattatac
    23281 cgcactaaag gtggagaagc aggcgaactt cacgccagac gatgacgagg gccagggcag
    23341 ctccggccag catggccgcc accgaggcaa tcgtttggcc cagcagcgga gccgccagcc
    23401 agagaacacc cagggcaagc ccggcgccca gaacggcggc aaggccgccg cgcacgaggc
    23461 tgctgcccac ctgcaagttc tgcggcagct tgcgattgag gaagatatag agcagaacgg
    23521 cctgcgaggt gaaggcgcca gcctcagcca ggctgagacc gggtgcgccc agaaaattgg
    23581 acagcgtgag tcccaagacc aagtaaacag tcaggttgag cgccccggtg cgcatggggg
    23641 tgcgggcatc ctgggcggca taatgggaac ggctggcgac ttccatgagg gcatgccccg
    23701 tgagagccgc aaaatagccg cgggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23821 nnnnncgcgc caggcccgga atctggatgc ccaggtgcat gagcgcgccc aaaatgacgc
    23881 cataaaccag accctggacg cccagaccca aggtgggcag ggtgacgcca gcaatggtca
    23941 gaccttcggt gggcgaaaag aacaacgcgc cgatgatttg gccgatattg tagagaatgg
    24001 gcgccagggc cgggagcagg aaatgttgat tatgctgcaa gcccgcgact accaggccgc
    24061 taatggagaa gattacggta gcaatcaggt tgaggcgcat caagttaatc accaggtgct
    24121 gctggtcggg ggtgaagcca gaggcaaccg ctgaacgaac aatcacatcg gcgagtaagg
    24181 cgatgatggc tgccatcacc aaagtagcca gaaaggccag gttggcaatc cggctgaaga
    24241 ggtcccaggc ggcacggcgg ccatcgcgag agagggtttc gctgagcacg gggatgaagg
    24301 ccatggccag gccgccgccc gagatgagca ggaaaagcat gtccggcacg ttattggcga
    24361 tgttgaacgc atccagctcg gaagaaaggc caaactggcg ggcaatcaac acctggcgga
    24421 cgaaggccac aactttgtca atcgcaaaga gcccggccag taacagggcg gcacgggtaa
    24481 cccggttggt atgaggtttt gaattatcca agcgcaacat ccaggagcat catcacagca
    24541 aagccgagca tgacgccaat agtagaaaca tccgtgtgtt cgccctgttg ggcttcgggg
    24601 atcagttctt caacaacgac atagatcatg gcgccagccg caaaggcaag cgcgtagggc
    24661 agcagagagc gcatgagcag cacagccgcg gcgcctataa caccggcaat cggttcggca
    24721 accgctgaaa gctgcccaaa aaagaagctg cggcggcgtg aaaggccttc tcgccggagg
    24781 ggggccgaaa cagccagacc ttcgggaaag ttttgcaggc caatgccgag cgccagggca
    24841 attgcaccag cgaccgtggc ttcttgcagc ccggcagcag ctgcgccaaa tgccaccccc
    24901 accgccagtc cttcggggaa gttgtggagg gtgatggcaa gcatcaagag gatgctgcgc
    24961 tgccatttgg ttttgacccc ctcggccttg aaaccgggaa tattcaagtg aatgtgcggc
    25021 agggttttat ctacagcgaa gagaaaaccc gccccggcga ggaagccaat caccgcgggc
    25081 atccattccg ggacgcccat gccagcagac atctcaattg ctggagcaag cagcgaccag
    25141 tagctggcgg caatcatcac gccggcggca aagccgagca tggcattgag caaccggcga
    25201 tctacctgtt tggtgagaaa gacacccgca gaccccagag cggtaacgcc ccaggtaaag
    25261 agggttgcca ggagggcctg cacaaccggg tggagcgatt ccatgaaagc aatcatcagt
    25321 gcacctcttt gttcaggtcg aatggaatga ttatacccga ttgttttgtc aagctttggc
    25381 aagcagtaaa attacaggct gatgacctta cctgcctgct ggagtgattc cagaatatcg
    25441 cccatgccgc caacaatgcc cacctgcaca tcatctgcca ggttgtaggt atccaggcag
    25501 gttttgcaga gaaccagttc cacgccgcgg gcggcaagct cgtttagctg gctgagcaca
    25561 ggggaaccgg tgcaggccaa gcggacgcca tcggtataga ataaaatccg agcgggcagt
    25621 tggccagatt cgagggtgag ggtgaggaat ttaaccgcca gggcttgctg caaggcctcg
    25681 gggccgtggc ccaatccaaa acgagtgaac agaacaaccg tattgcgatc catattaacc
    25741 tccaaaagac aaaaataacc caccggtttg caccggtggg caggtggagg taaccccagt
    25801 tctgctcagg cagtcggcca accgctgccc ggtgcaaaag ccaaacgctt tcggcgccgt
    25861 ccggcgcact ggaagcgagg tcaggtgtag cggtgggggt gactgaacat gatagagatt
    25921 atagcggatt ttgtggcata atgggattat cagtctgtga ttatcatttg aagccatcca
    25981 gaggaggctg ccatgcaaac ctatcctatc ccgatggtgc cggggccagt cagtgtgccg
    26041 ccagagattc tggctgagta tcaggttgat tttggctcgg cagacctgga accagagttc
    26101 ttggcgctgt atcagcagac agaagcaagt ctgcaaaaag tgatgcggac taaaaattct
    26161 gtggcaatcc aatctggcga ggggatgctg gcgctgtggg gagcgttgaa aagctgtctg
    26221 cagccggggg atcgggtttt gagcgtggca accggtgtgt ttggctacgg cattggcgac
    26281 ctggcccgga cggttggggc agaagtgcgc acgattgggc tggggcatga ccaaacgata
    26341 gagaactggg aagaaattga acaggccatc cttgagtttc gacccaagat gattacggcc
    26401 gtgcactgcg agacgccatc gggaacgctc aatccgctgg cgcagttggg ggagttgaag
    26461 cgcaggcacc aggtgccgct gctttatgtg gatgcggtgg caagcatcgg cggggcgcca
    26521 gtgttgacgg atgcgtggca gattgacctg tgcctgggcg gatctcaaaa ggcgctctcg
    26581 gcgccgcctg agatgtgctt cctgtcgatc agcgaggcgg cctgggagat catcgaagcg
    26641 gtagggtacg cgggctatga tgccttaaag cccttccgaa ccgcggtgcg agacttctac
    26701 tttccctata ccccaagctg gcatggggtg gcggcgctga atgtggcagc cgggcggctg
    26761 ctgcacgaag ggttggaata tgcctgggcc cggcacgaag ccgtggcgca gtactgccgg
    26821 gaaagagttg aaaaaatggg attaaaactg ttccccgcgg ctggggccat cccggcgccg
    26881 actgtcacgg cggtttatgt gcccaaaaac ctaagctggt gcgagctgga caaacggttc
    26941 agggcgcacg ggctggtggt gggtgggagt tacggaccgc tggcagagaa ggtgttccgc
    27001 ctggggcata tggggtctca ggccaaccgg gagctggtga gccgggcgct ggatgtgatt
    27061 gctgcggtga tttagaggac cggcggcagc acatggccgt ttttacgacc ggacaacagg
    27121 gttagaaccg attaaccggg caactgattc caatatttac gcttgaggcc atcggtgggc
    27181 tcttcaaaaa cagaccactg gcagccctgc gccatcagaa attgatgcgt ggggttgtct
    27241 ttatcgttat tgccggggca gagtaagatc aagccgccgg gacgggtgac ccgcaccagt
    27301 tcctgccatt cggcagtcag ctcatccccg aagacatgcc cacccatagt gatatcggca
    27361 aaggcagcgg ggaagggcag ggtggtaatc aggccatcga ccgcgtagaa atttgtcagg
    27421 ccgagggcgg ccgcgcgggt gcgcatataa tcgcgcaaat tggttacggg ttctacggca
    27481 tagacgagcc gggcgagcgg ggccacagtc aaagcgagcc gcccggtgcc tgcgcctacg
    27541 tcaatcacgg ttttgcctga aaaatcgacc atattcagca gttcggctga gtcccagcgg
    27601 gtgaatggct ggcggtcgta acattcgggc tccagtacat agaccagcca atcttccaac
    27661 gcctgcagga cctggacttc agcggtgcgc aacgcggctg gattttcggg ggcgggatgg
    27721 gcagacatga tctccgccag ccagggggag atttcggggc acttgtgctg caggaaccag
    27781 agcacagccg ggttgccatg caatgcagcc gccagagatg gaatggggac attacctacc
    27841 ggcagccaga ggatttgaac ccgctccaga aggagcaggg cgttgaatga tgtggtgctg
    27901 aaatctttcc aaccgagggg catgagaaaa ctccgaaaat gtgaacgtca gagaacagag
    27961 tatatcaggc gggtggctct tttggtagaa tagaaccagc ttgattttaa cctgtatttt
    28021 ctggagatga agcatatgaa aacccgctta tggctgacga ttggcctggt gctgatggcg
    28081 ctgaccattt cagcctgttt ttgctcaatg ccggatgagg attgcccgga cggcatgtat
    28141 tatgatgatg aatacgaaga gtgtgtttat gaggatgagg aagacgccac caacgctgac
    28201 ccaaccgact gggatacaga agaaggcgat gacagtgacg atgggaatgt ttttgaagat
    28261 ccctcggatg atgaagccag cgcttatgac gatgaattgg tgctggcaga ataccaggtg
    28321 gacggcgatg aattatacaa tgctgatcta cccagcgaca ttccggatga attggtgaag
    28381 tatcaggaag atgaggatgc gcattacgaa gcctgggaag ttttcactca attgatgccc
    28441 caggaatacc gcgagagcgt cacctcattc gcgatcttca cggatggcga gtcagaaacc
    28501 ctggcctggg ttgagccggt taccgacgac ctgtctgaat ggcggttggg gctggatgtc
    28561 attgatgtgg agagcgaccc ggatgaattt ttgtacactc tgctgcatga gtattcccat
    28621 atctttacgc tgagcccgcc ccaggtggac ctgggcggcg gaacgtgctc ttattatgag
    28681 acagatgaag gctgcacaga gccggactct tacctcagtg cattttatga gcagttctgg
    28741 actgatatct gggatgagtg gcaggctttt caagatgaac ctgatgagtc gctttacgaa
    28801 gattacctga ccgagtttta tgaagcgcat gaggatgaat ttatcaccga atatgcagcc
    28861 accgacccca gcgaagacat tgccgaatgc tggtcctatt tcatcatgga tccccgcccc
    28921 aacggcgata cgattgcgga agaaaaaatc ctgttcttct acgactatcc cgaaatggtt
    28981 gaaatgcggg atgaaattca agcccggctg gaagaatatt ttgggcgtta gtgaatgaaa
    29041 cgcaaaggct gattctgcgt aaatagagta tctgaaatta cgcttgaagg agttcttatg
    29101 aatacccgtc tcactcgcag taaaactgag cggatgctgg ggggcgtttg cgttggattg
    29161 ggccgctatt tgaagattga tccggtattt gtgcggttat tcttcattct gctggctatt
    29221 ggtgatggaa ttggcgtgct gttgtacctg atcctgtggt tggtgctgcc cgaagagagc
    29281 gccgaagaag ccagcctggc cgaccgggcc cggcaaatgg gcgcggattt tggcacagcc
    29341 atcagccacc ccaaccccaa gaccggaatg tacttgggca tcgggttgat tgtgctggga
    29401 gctgtgtatc tgctgaataa cctgaatatt cccggactgg cctggctgaa tctgcattac
    29461 ctgtggcccg tgctgttgat tttgggtggt ttggtgctgc tgctccggcg ggatcaatcc
    29521 cagccctgag cataggtgat tgaacgctga tttattctgc ggcagtttga cgaactgccg
    29581 cagatttgtt tacggagcag ggcaaccggg gttacaatcc atttgaggaa accataccat
    29641 gaaactgatc tacgaaaacg atttatctga ggcgggtgaa tttaagcgct ttgcgcgcca
    29701 ggcagagaca gcagcttttt taggcagccc ggctctgtgc ctggatggac tggtggtctt
    29761 tccaaactta gagctgacct gtggattgat tgaggttgaa attgcggctg aaaaatcctg
    29821 ctatccgggg gtggcttttc gagtacagga tcaaggcaac tacgagttag cctatgcgca
    29881 accccacacc agcggtcagt gggatgccat tcagtatgac ccggttttcc acggcaccaa
    29941 tacctggcag ctgtatcatg gggcggctta ccagcaaact gcccaggtac ccacgcatga
    30001 atgggtcagg ctgcgggtgg gatttaccga gaaccggatg gttttctggg tcaatgagca
    30061 gccacccctg ctggtggaac atctggctca cccaacccgg gcaggctgga tgggattgtg
    30121 gacgtatttg ccagcgtatt atcgcaacct caagatctgg gatgcgccgc agttccccga
    30181 gcagcctgga attgcgccca actgtccgag tggaacggtg actgagtggc aggtggaagg
    30241 ttacggcgtg ataccctgtg aacccaatgg cagccttaat gtcagtcgat atctccccat
    30301 ctcagcacag caggtgcagt tgaagcgcag ctttgagatc agtgcgggcg aggtgctggt
    30361 gaatggacca gtcaaccttg gttttgggtt cagcgacaca ttagcccttg agctagatgg
    30421 gcaggtcatt tatcaaggtg aaaacaagtt cggcgggttt gaaacccggg agtcgcgcgg
    30481 ctatgtggat cagaaaagcc atcacctggc gctggagatt gcccccggac agcacaccct
    30541 gacggcaaca ttaggcatca gcgaatattt tgggtggggg ctggcgctgg caatggaagg
    30601 gccggcaatc cactggctgc ctgccgggct ggaataaccc gccaggcaag ccagtagggg
    30661 cggatcaatg ctccgcccca tagatgacgc ggatgccgtt gttttcaacc tgccagaggg
    30721 ttgtggcaaa accatcaata aagtagcggt catgcaccac cgccagggca gtgccttcga
    30781 aggtggtcag agcctgctca aaacgcgccc ggctggggat atccagatgg ttaatcggct
    30841 catccagcag gaggaaattg cagccgctga ccaccagtgc acccagcgag aggcgggccc
    30901 gctcgccata ggagagcttg ccgaccgggg taaagacatc atcctgtgtg aagagataaa
    30961 gcgataaaaa ctgacgcgcc tctgtctctg ggaagccagc cagccgttga atcgtttcaa
    31021 gcgcattgag ggttggatcg agatcttcct gctcctgacg tagatagccc aggcgcacgt
    31081 tgacgcccag gcgtacctgc cccgagagcg gcggcagcac tccggcaatc gtccgcagca
    31141 gggtggtttt gccgctgccg ttggggccaa tgagggcgac tcgctcgccc aggcgcagac
    31201 gcagattcag atcactcaac aaagggccat tggcgtaacc gatggcaagt tcttcagtcg
    31261 ccagcacatc tttaccgctg ggcggggtgg cgccaaacgc aactttcatt tgccaggtac
    31321 gagagggacg atccacgcgg cctgcggtgc gcaggtattc caggcgggct tcaagcgcct
    31381 tggcgcggcg catggtgccg aggctacggt tggcaaagaa ccccttggca aacttatcgc
    31441 cggtatcggc tttgccgccc ttgcggaatt tcgccaggcc gcggatattc cgggctgact
    31501 gctccaggcg ggcaatttca acctgttgat cttgaaaagc ctgccattga tgttcgcgct
    31561 caatcagttt ttgctccagg tagtcggcat aattgccggc ataggcgcgc agcgtgtgcg
    31621 tcagcgggtc aagctccagg atgccagtgg ccacctgatc cagcagggcg cggtcatgcg
    31681 agacgagaag caccccaccg cgaaaactga gcagccagtt ttcgagccat tcgagcatgt
    31741 tcagatcaag gtgattggta ggctcatcca gcagcagcaa ctggggattg gaaatcaaca
    31801 ccccagccag ggccaggcgg gttttctggc cgccgctaag gctttgagac agttggtccg
    31861 ggggcaggtt gtctacaccc agggctgcca gcacagatgc ggtgcgccca gcgttctctg
    31921 cggccacctg gatcagattc agcaactggt catagcgctg ctgatgttcg ggagtggtca
    31981 gccggggagc caattcttcc agttcagccg tcaaagcggg cagatcgccc tccatgcggg
    32041 caatgaaacg ggcaattgtt tcgccgggtg gataggtgaa tccctggggc aggtacccga
    32101 cctgcagcga ggacgggatg aagtggaaac cgccctcatc gggtttttca aggcccatga
    32161 tgatgcgcat caacgtggtt ttaccacacc cattggggcc aaccagaccc aaacgctcac
    32221 ctgaattgac ggtgaaattg attttactga gaacggtgtc gagaccgaat gattttgaaa
    32281 tattagaaac tgagagcata taacctgtac tttcgcgatc caaataaaaa gccggaggct
    32341 gctgcctccg gcaagaattt caacgccaag ctgcgcgaaa cacaccgata cagaagccta
    32401 atcgcaaaga aaattacagg ttgctatccg tcatttaata cactcctact aagccgacgg
    32461 catgattata ccacggcgct ggcgtaatga tgaaggttga caaggcggat tcaagctgag
    32521 gcactgcgca aggtttcaag caggctgaga aggacatcct tgcgatcttt gagcatatcc
    32581 ttgtagtcaa tccggtcggt gtcgacaatt tccatgcgca ggtcagagag acaggattca
    32641 aggagttcga caagcagttg atattgttca ggagatagtt cgaggcggac catggcacac
    32701 gttcctttct gccagccgcg ccccaatctg gcgcggaatt ggcctcactg atacgatagc
    32761 ataaaccgcg ctctaaaaca accggttaac ggcgagctga gtcgaaaaat gcaatgatac
    32821 gctgttcata ttcgagcggc gccagttgag catagccccc atgcccggcg ccagggacaa
    32881 tccacatctg ggcggcaggg ccggctgcgg ccagttgatc gaaggcccgg ttattatcag
    32941 cctcaaattc gccaaagatg agcaagacgg gacgcggcgc aaggtggggc agggtgtcga
    33001 ggggggaaat ctgacgaggg ctgtgaccca gcgcaaccca aaacaagccc acaacccact
    33061 gctgcacctg ccaggctggt gacagcaccg ggctgttggc attggtaatt tcatagtgca
    33121 ggctggcata atgcccttca gcaaccacag cgtcaatgcc gggcagatcg gcagcgccaa
    33181 gaatggcagc ggcaccacca gccgaaaagc ccaacacccc cacccacgcc acaccaggct
    33241 gggattgagc aaagtccagc atggcggcga ggtcagtggc ttcacgatag cccagggtgg
    33301 ccgcgccccc ctgacaattg cgatattcaa gcgttaagat gccgtagccg tgccgggcca
    33361 ataattcggc atccccaagc atggaatcac gggaaccgcc gttgcccgcc agcagcaaaa
    33421 tgacggcgcc gttctgcggt gggcgccacc agccgcgcaa gtgatagcca tctgaggtgg
    33481 cgatggtgac cgattggtag ccggggcgtt cggcgagaga agccgggcag ggcgggacca
    33541 tcagactacg aacatagatc gcgctgagcc cgccgaggga gaatgtccag gccagaacaa
    33601 agatcaggag cagccgcgtg accagccagc gcaggtggat tggccgggac aggcggcggc
    33661 tggggttatt tttctgccca ggcatccagg gcccgcagta aattttccac atcggcgggt
    33721 tgaagctcgc ccatggtggc aatgcggaag gtggtttcgc gcaggcggcc atagccattt
    33781 gctatacgca tgccgcgctg gagcaggaat ttattcaggt cagtcatatc ccagttgtcg
    33841 gcattacgca gaacagtgct ggttttggag gtgtaaggct cgggtgccag agtgggcagg
    33901 ttgtgcccgg cagcccattc tcgcacacgc gccgagagcg ccgcgtggcg ctcataacgc
    33961 gcctcaaatc cctcggccat catccggtca agttgggcat ccagggcata gagcagcggc
    34021 atggcgggcg tgctgggcag ggaatctttg aggcggtggc gctccatgcg caccaggtca
    34081 aagtaccagc cgcggtcggg tactgcttcg gcacgctgca tggcccggtc agaggcggca
    34141 gccagtgaaa gcccgggcgg cagcgccagg cagtgctgcg aggcggcaaa gagaaaatca
    34201 atcccccagg ggtccatttc cagcctgaca ccgccaaagg atgagacagc atctaccagg
    34261 atcaatgtgt cggggctggc ggtgcgaacc gcagccgcaa gttctggcag cgggttttca
    34321 accccggtgg aggtttcgtt atgcacgatg gcaacagcat cgtattggcg ctgagccagg
    34381 gcggtggtca attcaccggg caggatgggc tgaccccagt cggcgtccag ccggtcggcc
    34441 gatttgccat tggcaatggc gatatcgtac cagcgttctg atgatgcgcc gttgacgcaa
    34501 atcagaactg cggaggagac aaaattacgg atcgaggctt cctgcatgcc cgtgcccgaa
    34561 aatgagccct ggaaaacacg cccgccaccg ccaaacaaag gctgagcctt ttcggcggtg
    34621 gcgcggaaga agttctcaaa ttcgctgctg cggaatggca atattgggcg ggcctgggcg
    34681 gcaagcacct caggggcgac atccaccgat cccggaacaa acgaggggga cacgggaggg
    34741 gtttagatta ccgcaggaag aacgacagga cgatcagaat aacaaagaag gaacccgcca
    34801 acagaccaat acggcgaagg tccttaatga cgtaatcgta attggggttg aattccgtag
    34861 tgcgggcggc ggtggtcacc ggggcaaccg gggtggggga agtctggctg atgccattgg
    34921 tattgcgttt ctgctgcttg ctcatcaaat tctccttaaa gatagaaaat tatttttgct
    34981 ctaacaaact tgcggtgaca acaatccgca tattgtcttt gagatagggg taacaggaaa
    35041 tcagggtcac aatgggctcg gttgtctgcg ccattacctc aacgcgggtt ggctcaacaa
    35101 tctgggtctg ttcaacgacg tacacatagg cccgcaggct ggtgtagacg atgatttgat
    35161 ctccgggttc cagcttatct aaatggcgga aaatttcgcc atagacatca ttatgggcgg
    35221 caaggattaa gttgccgttt tggccgggat tgacgctgta aatgttctgt ccaacgcctt
    35281 ttttgagctg ctcgtcgcca tcaccctgga ccaccggggc atccacatca atatcaggga
    35341 tctggaggcg ctgggcctgc tcggggccgg gggtggggac gggcaggttt gccagggttt
    35401 gcgccagggg gcgcaaatgc tcgggaattt cagcctcgtt ggggcgcccg ccgccatcca
    35461 cagcattggg gggggtatgg cctgacggca ggacaactgc catgaccagg ggggtgggcg
    35521 tgagggtggg ctgttcaatc agggcagcga cctcagagtt gaggttgcga agaatattca
    35581 tgccgttgaa gaggatgaag atcagcccga tgactgccag cacttcaatg gctgaaagaa
    35641 aactattgag cacgcgctgc cgtttggagg gtgcggccgg ggcgccagca ggcaggtcag
    35701 cttcttcagt cggaatgctg cgcaaaccgt caattggcgc gggagccggc aggggctcaa
    35761 ccaggatcat gcggccggtg cggcggaagt gatcaatgcg ggcgcggcgt tcagcccgtc
    35821 gcttttcaat aagcaactgg cgaagttcgt caaccgtcaa atcttctacg cgccgatgcc
    35881 gagacacacg tcttcctttg aaccagattg gcctgattat aaccgatttg cgggtctatg
    35941 gatgattaga ccggggcgct ttgcggcgcg ggtgggcccg tttgacgggg ccgcgacggc
    36001 gcgtcatggc gcggcctgcg ttggggctca ggatcaggct gagttggttg agggcgcggg
    36061 gatcatattc ccgccgcccg caaacatcgc aaacccaggc cggaaagtca gatacggtga
    36121 tcaactggtc tgccagccag gtgaagtagg tgacattctg caggtgcatt tgacccgccg
    36181 gacattcatt gcacggaatg gtcggcgggc gcgaggaggg agccggaaac tcagacgttt
    36241 tgagactcct gaacaatttt gatgccagaa ctggtgccca gccgactggc accagccgcc
    36301 agcatggcgc gggcatcggc gagggagcga acccccccgg aggccttgac gcccaggtcc
    36361 aggccaacca cgcggcgcat cagttcaaca tcctcggggg ttgcgccact ggcggcaaac
    36421 ccggtggagg ttttgacaaa attggcgccc gcggcgcggc tgagcaggca ggccaaaatc
    36481 ttctcactgc gggtgagcag ggcggtttcg atgatgacct taaccagcgc accacccggc
    36541 ttagctgcct gaaccacggc agcaatatga tcgtgtacgg cctgggcttc gcccgctttc
    36601 aacaggccca cggggatgac gagatcaatc tcgcgggcac cggcttcaac cgcctgacgg
    36661 gtttcaaagg ctttggtttc agcagggaca gcgcccagcg ggaacccgac cacggtacag
    36721 gtggcaacag gggaacccgc aaggcggcgg gccaccagat cgacataaat ggggttgacg
    36781 caaaccgcgg caaaacgata ttgaacggct tcggcgcaga gcgcttcaac ctgggccggg
    36841 gtggcctctg gcttgagcag ggtgtggtca atcatcccag ccaggccagc cgggtcagcg
    36901 gcatattcgg gctggaaggg cggaagctgg gcttcgtagg actgggcaag ttgtaatagt
    36961 tgattaagtt tcatggggta gaggcagcat tcacaatatc aggaccattg aggaggcgga
    37021 cttcgttcag cggccaatag atgaccagag cccggccaag cagattttcg ataggcacaa
    37081 agccccagga gtgcgaatcg gaggattggt tgcggttgtc gccgaggaca aagacagagc
    37141 cttcgggcac gttccaaacg ccggtataag acggtggagc ggcaatatag ggctcagtca
    37201 gggcatagcc attgaccagc acctgaccat tttcaactcg cacctcgtcg ccgggcaggc
    37261 caatcaggcg tttgatgtaa tcttcagtgg ggttgccggg atagtggaaa acgacaatat
    37321 cgccgtggtg catgttcccc aggcggtaag ccagtttgtt gaccagaagc agctcgtcgg
    37381 ctttgagcgt gggctgcatg ctgatatttt caacccgaac ccggcccacc atgctgtcaa
    37441 tcaggaagta gagaactgct gccaggagca gggtctgtaa aacctcactc aaaaaggttt
    37501 tccaggcggg tttcttttta acctccgggg gatcttgcgc caccggttcc gcgcgatagt
    37561 cttccaaaat acctccgata caaccgctaa tgcctgtgaa caggtcgtct gggttgatta
    37621 tatatccgct tgtcaattgc gacaagcgcg cgattaatgg gtttctgccg cccgcccccg
    37681 cagccgggtg ccgcacaccc gacagtagag gtcgctggct tcagcacgct tgccgcattc
    37741 cgggcaaaaa acggctgtgt cgtgagagag gttgtttttg gggcgcaact ggcgcgccca
    37801 gactgggcgg tatttctcaa cccattcggg gttcatgcgc cgccaggtaa tccagacacc
    37861 gaggatgagt gcgacaagca gcagaaatcc ggcaatcagc caggggagaa tagtcaccag
    37921 acgcgtccga ccggcaatat gttcatccag gggtgaggcc gcgctgacag gctgggtggt
    37981 gaaggtgagg gtgctgtcgt ttttctggta ggtcagcgcc agattgaagg gcatgccaac
    38041 atccaccttt ccaatggtgg tttcataata aatgaggcca tcttcaccca gagagccgcc
    38101 ccccaggtgg ggggtaattt ccatctgggt agcgccaatg gggttttgaa tccggacggt
    38161 taaatcgtca acagtgaaat ctgccggcca aatgtaggta taggttcggg tggcttcatt
    38221 aataagcagc cggggatcat aaaattccag gcggatgagg ctggtggggg ctgtcagttt
    38281 gatctgcgtc caatccgcgc ctgaaatggt ttgaacgttc agtggcgtgg gggcagaatc
    38341 cgaactgccg acatagtacg acgcctgata gagcgtggtc gctgcccgcg ggatagagag
    38401 gctgatggtg gcaggcagct tgacctgcgg tgacaggcga atggtataaa tgaccaggac
    38461 ggcggtgcgg tcatattcag gccaaatatc tacaaccacc gcctgggctt caatgggggc
    38521 tttatttgtc tgggccgcgc caaacagcac tgagctgagg gttaggaggc tcaagatgag
    38581 ataaaatgct gaccggcttc tgggcataac tcaatcttac catgaatgca ggtgggtgat
    38641 gatgaggcgc agttaaattt tttagttgtt cgaagcggtt ttctggctgt gatttgagcg
    38701 tttctccgct ataattggtt tatgaaaatg gggcggacaa aggtccggcc gcgcagacac
    38761 aaaatagaaa agaggttata tgggcgactc ctcacgcttt tatctgggta aaaaatttga
    38821 tctggcaagc ggcaagctgc tggatcaacc tgtgctgtac gatccagccg acctgaccac
    38881 tcacgccatc atcaccggca tgacgggctc gggcaagacc gggctgggga ttggaatgct
    38941 ggaagaagct gcgctgcagc gtatcccggc gattatcatt gatcctaaag gcgacctgac
    39001 caacctggtg ctgcattttc cagatttgct gccgcaagac tttgaaccct ggctggatcc
    39061 agaagctgcc cggcgggcgg gaaaaccgct gcccgatctg gccgccgaaa ctgccgcccg
    39121 gtggaagcag gggctggcag actggggact gggccgggag cagcttttgg cgctgcagca
    39181 agccgcccaa tacagcatct ttacgccggg ctcatcttca gggctgccag tgaatatttt
    39241 gtcatcgttc cgggcgccag aaattccgtg ggaggagaac agcgaaattt tacgcgagaa
    39301 ggtctccagt acggttacgg cgctgctggg cctggtgggc attgataata ttgatccact
    39361 gcgctcacgg gagcatatct tgatttccaa tttactggaa catgcctgga gtcggggaca
    39421 gtcgctggat ttgactgagt tgattttgca gacccaaacc ccgccctttg accgactggg
    39481 ggctttccca attgacaact tctacccgga aaaagaacgg tttgaactgg ccatgctgtt
    39541 gaataacttc ctggcgacgc cctccttcca gatctggctg gaggggcagc cgctggatat
    39601 tggggcaatg ttgtacatgc cggatggccg cccgcggcat aacatcttct acctggcgca
    39661 tttgagcgac aacgagcgga tgttctttac cacgctgttg ttcgctgcag tggaaggctg
    39721 gatgcgggcg cagcgcggca ccagcggact gcgtctgatg gtgtactttg acgaaattat
    39781 gggctatttg ccgcccgttg ccaacccgcc ttcgaggccc attatgctgc ggatgctcaa
    39841 acaggcacgc gcttttggcg tcggcctggt gctgtccacc caaaacccgg tggacgtgga
    39901 ctacaaagcc ctatcgaacg cggggacgtg gatgattggc cgcctgcaaa ccgaccagga
    39961 caaacagcgc ttgctggacg ggctggaatc ggcctcgggt gcgctggatc gcgccgatgc
    40021 tgataaatta atctccggcc tgcagaagcg cgttttcttg ctgcataatg tgcaccagtc
    40081 ggccccggtt actttccaaa cccgctgggc gctcaattac ctggccgggc cgctgacccg
    40141 gggcgaaatt ccggcgttga acaaactggc aaaagccgtt gctcccacag ccgcgccggc
    40201 cgcggcggcc attccagtta gcccgcaggc tgcctcgatt gctgaaacgc agcccacccc
    40261 ggtggcggct gcggctgccc caaaaccagc cgcaccggcg ccgaatgggt cacaaacccg
    40321 cccggcggcc ccggtgggtg tgatggagtt cttcctgccg aatgatctgg gtatcagtca
    40381 ggcactggcc gcggcaaacc tgcaactggc cggactgatt gaaccagaag ggatcttgta
    40441 tcaaccggcg ctgctggctc aggcgcaggt tcggtacacc tcacggcaat ataatgtaga
    40501 cagcgctcgg cgcgtggcgg tactgctgcc ccaggcggat gccgggctaa tccgctggga
    40561 ggaatatccg tggggaccgt atgagccagc ggctttaagc agccaggccc tgcccaaggc
    40621 ccgctttgaa gcgctgccag cctggctgac cgatgcacgc cggttgacgg ctctgcaaaa
    40681 agattttatt gaatgggttt atcggaacgg cagcattcgg ttgcgctcca acccgaccct
    40741 gaaactggtg gctggcccgg atgtcagtca ggcgcagttc cgcgaacagt gcagcagcgc
    40801 agccaagcag gccatgcagg ctgaagtgga taagttggaa accacttacc gcaccaagtt
    40861 ggagacgatg aagcagaaga tcaagcggca ggagaaagag gttgaaagcc aggaagatga
    40921 agtatcgcag cggcggaccg aagaactggg cacacatggc gaactggtgc tcagtctgct
    40981 gggtggacgc aagcgcagca tctcatcatc actgaccaag cgccgcctga cccagcaagc
    41041 caaggccgat ctggaagaag aaaagcagga actggatgcc ctggaagaac aattgattgc
    41101 catggaagcc gaacacaagg ccgcgctggc ggctattcaa gagcgctggt cacaggccgc
    41161 caatgatcag agcgaagtgc cgctatcgcc cttgaagaag gatatttttg tagaggcttt
    41221 tggaatcgcc tggacaccct actacttgat ccgcaacggg gataaactgg aaaagctggc
    41281 ggcttttacc gccccggctt agagagcaga acctttttga gataaaaaat cgcccggcag
    41341 acgttcgccg ggcgatactt tttcgggtta tcccatttcg ctgcgccctg aaagggctcg
    41401 cagaagggtg ttttggtcgg catattccag gtcgccgccg acgggcaggc cgcgcgccag
    41461 acgggtgacc tgcaccccca ggggtaaaag ctgctggcgc agatagagtg cggtggcgtc
    41521 gccttccatg ctggggttgg ttgccagaat gacttctcgg acgccgcctg cctgaatgcg
    41581 ctcaatgagg ggacgaattt tcaaatcctc ggggccaatg ccctcaattg gagaaatgac
    41641 accatgaagg acgtgatagc ggccctcata acccgcggta cgctccagcg ccaggacatc
    41701 gaggggttct tcaactacgc aaatcacgct ggcatcacgg cgagaactgg cacaaatttc
    41761 gcagatgggc tgctcagcca gcgagatatt gaagcaaatc tggcagagtg cggtggatga
    41821 tttgagtttg agcagggcct cggccagctg gccggagatt tcatcggggg cgcgcagcag
    41881 gtaaaaggcc aggcgggaag ctgacttggg gccaatgccg ggcaagcgct ccagggcagt
    41941 gatgagattc tgaacaggca gaggtaagac gggcataagc tgcgcctaga acaggcctgg
    42001 catacctgcc agaccgccag tgagcggttc catgcgctga gcagccagtt cgcgggattg
    42061 atccaaagcg aggttgacgg cgctgaggat caggtcttgc agcatttcag catcggcgtc
    42121 tttcaagact tcggggtcaa tctcaactga acgacagacc tgggcgcccg tcatggttac
    42181 cttgaccacg ccgccgccaa cagtggcggt gacggtttct tcggccaatt gggcctgcgc
    42241 ttgcgccatc tgttcttgca ggcgttgaat ctgctgcatc ataccgccgc cttgattacg
    42301 gccaccaacg gggcgattaa atcctttagc cattctttat actcctctac ggttattctt
    42361 tttggactat tttaccaccc aggttgaggg cggtgcccac cagtccatcg gggtcttcgc
    42421 catcagcaga gagccctcca ccccgagcgc caaccaccac acagcggatg gcggggcgca
    42481 ctcttaaaag ctgggctatg gcatcctgag tgatgcggat gttatcattg ctttccattt
    42541 tggacttaac cacctcagtg gaaaagccca gaatcagcac accttctcgc agcgaaatgt
    42601 tcttgcaaga gttcagcagg gcttctgtag gcgggcgcgt ttttttcaca atcatgcgga
    42661 tttggggcca ggcctgggtc agcgcctgaa gggtcagttc tccaccagtc aggggtgcgg
    42721 ccggggttga ggcagcctgc gctgcagcgg gttgctctgc cgcgggggtt ggagagcgct
    42781 gctcgggcac tggttctgga ggcaaggggg gtggggcagt cacgggcgcc tgttgaggtg
    42841 gcgctgactg tgccagaggc ggagtgatga tcggttctgg ataagccacc gcctggggaa
    42901 cggacatctg gcgcatttcg gcctgttcca tggcggcaat tgattcagcc agggctactt
    42961 caagcaagag gcccggctgc cagccgctgc gggcctcatt ggcggcggcg ttgaagagcc
    43021 gcacaatttc aagcagctgc caacgagtaa aatcctgagc cagggcagcc atctgggcgc
    43081 gggcttcatc ggttgcatcc accaggcggg cgttgcccat cgagaccagc agcaacgcac
    43141 gcagataatc aatggtttgg cgggcaaact ggcgcgggtc ggtgccgctg tccaaagcct
    43201 gatgaatgct gtccaatcca gcggcaggct ggcggctgcg gatggcgcca accagatcaa
    43261 gtaccgattt gctggtggct gtgcccagaa ctgtttggac cagatctaag gaaatgtgct
    43321 gaccggttga ggccacctga tcaagcaggg agatggcatc gcgcatgctg ccggtagcct
    43381 ggcgggcaat cagcgtcagg gcatcttcat cggctttcag cccttcgtta ctggcgattt
    43441 cttgcaagtt ggcaacaata ttgctgaccg ggatgcggcg gaactcgtga cgctgacacc
    43501 gggaaagaac agtggcaggg attttatgaa cctctgtggt ggcaaggata aaaatggcgt
    43561 gggcgggggg ttcttccaga gtcttcaaca gggcgttaaa agctgcggtg gagagcatgt
    43621 ggacttcgtc gataatatag actttataat gtccctggga aggcgagaaa ttgattttat
    43681 cgcgcaggtc gcgcacgtca tccacactgg tgtttgaggc ggcatcaatt tcaatcaaat
    43741 cgaggaagcg gccttcgttg accgcggtac aaaactcgca atggtcacag ggccgctggg
    43801 cgggatcggg ctcaaggcag ttgacggctt tagccagcag gcgggcggtg gtcgtcttgc
    43861 cggtgccgcg gggcccggca aataaataag catggccaac acgctcgctc cgaatggcat
    43921 tcttcaaagt ctggacgaca tgctcttgcc cgaccacctg ctcccagaga tgagggcgcc
    43981 atttacggta aagtgcttgt gtcataacag agttctcctc atcagggcag gcgcagttga
    44041 gcgcgctcat tgccctgtga atcggttaat aaaaccagct cgcccgattc ccagacgggc
    44101 tcggtcaggc cccagtataa gcctgcggcg gtgttacgcc cggcaagggt atagatctga
    44161 atggaacccg agttccagat catctccggg aaggtgaaga cgtgaccatc ttcatcccgg
    44221 agctgccagc cggtcatcca cagatcgcca gagccgatgc gcgtcagggt gatggattcg
    44281 gtggacacat cgcccaggcc aaagaccgag gtgatttcaa ccaggggttc acccagtggg
    44341 ggtacggtgg ccgttggcaa aggggtgggg ttgggcagca gggtcagtgc cagggttggt
    44401 tgaggggagg gctgaagttc tgccagcaag gcggtatact gcgggcgcac aacccacaac
    44461 cagaccagga tggtggtcag cgcggagacc agcacattta ataggagata agggatgaca
    44521 cgtttccagg acttcatgtt ggacctttat cagatgggct tgccctgccc cttgaatgat
    44581 agcacagaat gacggtaaaa tgggtaagat gccttacctg attgatggtc ataatttgat
    44641 tcccaaagtg cccgggctga gcctgggcgc aatggatgat gagttgagcc tgatcgacca
    44701 gttgcaagtc ttttgccgga taagccggca gcaggtggaa gtctattttg atggcgctcc
    44761 cgccggacag gcgggcaccc ggcggtatgg gcaaattccg gttcattttg tcagactggg
    44821 gcggacagca gatgatgcta ttgcagagcg gctggcccgg ctgggacggg cagcgcgcaa
    44881 ctggcgggtg gtcagttcgg accggagggt gcaggctgag acccgtgccc gcggcgctga
    44941 ggttgtgact tcagaagttt ttgcggctga gatgagggcc gctcaggcta gagcagatga
    45001 gcagcccggc ggcgctgacc gaaagttaag ctcagatgag gtggatgact ggctggatct
    45061 atttaaccgg gggaatgaaa agccaggcta aggcgattat caggtaactc ttaagtgtaa
    45121 ttaattgatt tttgataatt ttgatgtata ttatcgacaa gacaccggtt cgtttttact
    45181 ggtagaaggt gtcgaaccag attgctattt ctgcttgtcc cccccatgca gcagcatctg
    45241 gttaggttcc ctccctgaag attgccaggc atgtccccca tgcctggcaa tcgttttaac
    45301 aggcttgaag ggggataatg aagatgctat aatcaacact gccgccccag tagctcaaat
    45361 ggacagagca aggcactcct aaggcctagg ttgggggttc aattccctcc tggggcac
//
LOCUS       DAOPVS010000005        43348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_811_length_43348_cov_58.971381, whole genome shotgun sequence.
ACCESSION   DAOPVS010000005 DAOPVS010000000
VERSION     DAOPVS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 43348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43348
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_811_length_43348_cov_58.971381"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..571
                     /locus_tag="PKL11_01215"
     CDS             74..571
                     /locus_tag="PKL11_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12244.1"
                     /translation="MAKRTAKPKSKPRPETNSNLWLWLILGGAVLALVIVAITANPRQ
                     PSAAATILPPTVVLPTLTSSSPAAPITPAVTTEIPTVVAAAPDPAVLVTPDPTWPYPE
                     IPRVTLEGARAAFDSGAAVIVDTRSAESFAQSHAVGAINIPVNEMEALYTQLDPNQWI
                     IPYCT"
     gene            complement(743..2161)
                     /gene="glnA"
                     /locus_tag="PKL11_01220"
     CDS             complement(743..2161)
                     /gene="glnA"
                     /locus_tag="PKL11_01220"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNZ12245.1"
                     /translation="MFQDFIEAATFVRENDIRMIDLKFCDLWGRWHHVTISASEFTPE
                     LMEAGIGFDGSSVGFKSVKAGDMALIPDLTTGFLDPFWEMPTLSFICDTVEADTKERF
                     AGDPRIVVQRAEAYMQEHGFATESLWGPEFEFYVFNDVVLENTVNAASYRVDAYEGQW
                     NSVQGGDGHLLPLHGGYHAIPPRDQTYDLRARMVMTLEQVGVPVKYHHHEVGGPGQCE
                     IETPLMGLLAAADASLVIKYISKMVANAAGQTVTFLPKPLYGEAGSGMHFHQQLLRDG
                     ANLFYDAKGPNLLSKTALYYIGGLLTHAPAVLAFTNPSTNSYRRLVPGFEAPISAFFS
                     SGNRSAAIRVPKYATQPDTVRFEFRPPDATCNPYLAMAAQLMAGLDGIRRKIDPTPAG
                     FGPIDDDIFSWPAEKRATIKSLPTSLEEALDALEADYDFLLEGGVFTEEMLRTWVKGK
                     RAEDLQVRSRPHPYEIQMYFDL"
     gene            complement(2210..3427)
                     /locus_tag="PKL11_01225"
     CDS             complement(2210..3427)
                     /locus_tag="PKL11_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNZ12246.1"
                     /translation="MSTYPGKGKVAVLKTSPETVLEDIDRLMVMAGVEQALPKNVPTG
                     IKINISWQTWYPACSSTPWQLEGTIRTLQRLGYPDIMGIHNDTVVVNTRDGEANNKHR
                     FVTDKYNVPCMYLYEQQFEWQQYELRHGDYLVLDRVFPEGVFIPKALIGRNIVHLPTV
                     KTHVFTTITGAMKNAFGGLLHRNRHWTHSVIHETLVDLLRIQQEIHPGIFAVMDGTFA
                     GDGPGPRAMRWHEKNILLASADQVAIDAISAKLQGFDPMSIPFIRMAHEMGLGVGDPR
                     EIEIVGYDIEQEPAWHFKQEDTFASRGQKMIYHGPLKPLEKPLLQSPLVPWSFFASNF
                     YHNVYWYPFVGRRRVEEALQTQWGRLFRSYGDGEVVMPGMEPKTVITAAAATAAVAAL
                     AGLGIWALTRKKK"
     gene            complement(3508..4971)
                     /gene="purB"
                     /locus_tag="PKL11_01230"
     CDS             complement(3508..4971)
                     /gene="purB"
                     /locus_tag="PKL11_01230"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNZ12247.1"
                     /translation="MYKQRNSSKIEENDTGEDFMEFETYRSPFSFRYGSAEMRRLWSE
                     QEKRRIWRRIWLALAETEAEFNLIPTNALADIQAHVDDIDIERALEIESEIHHDLMAE
                     LRTFAEQCPTGGGSLHMGATSMDIEDNADALRIRQALDLLLPRLQALLLSLAEQVERQ
                     ADLPVMAFTHLQPAEPTTLGYRLSTYAQDLLEDYHALVGRRNQIRGKGFKGAVGTGAA
                     YGELVGVERLGPFEASLSARLKLPFYPVTTQTSPRKQEYTLLCALAGLGQSLYKFAFD
                     LRLLQSPPLGEWSEPFGKKQVGSSAMPFKRNPIQAEKIDSLARTLAQMPVTAWHNAAH
                     SLLERTLDDSANRRTLLPEAFLTCDELLITAEKMIKGLVINEAAIQRNLEMYAPFAAT
                     ERVLMAVAKRGADRQEMHERIRQHALQAWAAVQQGQPNPLLTLLMYDDLIARWLKVEE
                     LRPLCEVQGYLGLAPAHARQMAGRIRAELGEASHRNG"
     gene            complement(5029..7008)
                     /locus_tag="PKL11_01235"
     CDS             complement(5029..7008)
                     /locus_tag="PKL11_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12248.1"
                     /translation="MNKHLTVWLAMFIGFSLVLAACTPAPGALATAQPEAQSTPQPGE
                     EQTAAPVEYPTALPPTEAPRPEANQVISPENGAQLTLINVLGKGNITSSAWSPDGSRV
                     AVGAGYGIYIYDRQNLEMRMFIDTHSYITAVAYSPDGNQLFSGGSEIEVWDAQSGAEV
                     GQFEGSGGTATYLALSPDGKVLASSGADGVIHLWDVSTRAELRQLEGHTQQIHGLVFS
                     PDGTRLASAGLDHIAIIWDVNNATALHSLDHGNAPVYSVDFSPDGKTLASAGSQAFHL
                     WDVESGVETAKLEEQSLGLTMSIAYAPDGKTLVGGCFDGSVHILEAGSLQAVGRLEHG
                     SKVFGAEYAPDGQTIFSYSYDGVLHLWDVASGAELQQASGHINFIFSLAFAPDGTKLA
                     AGAGDGSMQVWAVPSGTPLMRLEGHTVQTRGAAFSPDGGILASGGNDAIIKLWDAQSG
                     AELRTLEGHEYLINSLAFSPDGSLLASGSKDGTVRLWDAANGAGLMELNGGMGAVSSV
                     AFAPDGSTLAAGGSDGSIILWSPVSGEEMRRLTGPAEGVLSLAFSPDGKMLASGGADG
                     KIDLWDANSGNSLGQLGGDMKSVNSLAFSPDGSLLAAGCASEDNLRLWDVNTATEILR
                     LRAHTSSITGVAFSADGMILASGAASGDVRLWGVP"
     gene            7263..9170
                     /locus_tag="PKL11_01240"
     CDS             7263..9170
                     /locus_tag="PKL11_01240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ12249.1"
                     /translation="MAGFCLEDHLDTHNITQNLNQGQSPPPKQLQPGYVLLDRYLIQE
                     VIGVGGMGSVYRARDLHFQNVIKLVAVKEMINSSPDPIVRQTIVQNFEREANILAALN
                     HPSIPKIFDFFSHDERSYLVLEFVHGKDLEALLNERDDFFPEEQIIGWAVELCDVLQY
                     LHLHKPEPIIFRDMKPSNVMVNQQGHIVLVDFGIAKSFRTGQKGTMIGTEGYSPPEQY
                     RGEATPLADIYALGATLHHALTRRDPRLEPPFSFAERPVRASNPGVSQELETIVTTAL
                     RYNPDERFPSAEAMKDALLTVARKTGVLSKISMPSTDRSSTTIKPLWTFKCEDEIRGS
                     AVFENGVVYIGSYDNNLYAVNAGNGQFIWKYPANGGIVGRPVLSEGNLYFGSEDNSLY
                     VISSRTGRIVSSFQTQGPIRSSPRIAEGHIFFGSDDGFLYAINQTSTRSSWRFEVGEP
                     VRSTPAASQDAIFFGTEGGEFLSVDFRGQARWRFRAKRAVTSSPVVTQGTVYFASVDS
                     SVYALDIRSGWMLWRFRMEKGSVSSPCRAENMLFVGSADGNIYCLDAGNAKEIWHFKT
                     GHQVSGSPAIYRDSVYCGSADGNIYCLEYRTGRLRWRFTTGGPITGTPVIVDDVLYIG
                     SADHLLYALLA"
     gene            9203..10105
                     /locus_tag="PKL11_01245"
     CDS             9203..10105
                     /locus_tag="PKL11_01245"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNZ12250.1"
                     /translation="MIQFFKNLFPRKKSEALPSAKDMIQTAPLDAESEDPAAGRSPYR
                     PAQFLVGCAQSVGIQRDHNEDTLFAMSSVLADGVNDIPFGIFIIADGMGGHQYGEIAS
                     GVAARTMANHLLARVYQPLTNPDLERSNDSLQELMETGVREAHQIVLRKAPGGGTTLT
                     AALLLGEQITIAHVGDSRAYFIYSDGRIRPLTQDHSLVQRLVELGQLTAEQAVNHPNK
                     NVLYRVLGQTEPFRPDIENRMMPHPGYLMVCSDGLWGVLPEDDLLKIVQQSSNPTEAC
                     HKLVEAANAAGGPDNISVILVQFL"
     gene            10118..11587
                     /locus_tag="PKL11_01250"
     CDS             10118..11587
                     /locus_tag="PKL11_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNZ12251.1"
                     /translation="MPSGADYYNALGLTIDATPEEIRTAYFEAARRLHPDINPDPKAQ
                     EIFLQIQTAYDVLSNPRRRADYDAGLPADLRARPDINLSVKYSRPAVPAIGEAQLFYV
                     LLDLNCTAELDATRTPPVNLCLVIDRSTSMRGERMDMVKANVVQLVRQLRPHDILSLV
                     LFSDRAEVFLPPMRVADINRLESQISMVQCGGATEIFRGLEAGYSLLTRYGDSRANRH
                     LVLLTDGHTYGDEAPALNLAERAAQENIATSALGIGSEWNDVFLDRLTSFSGGNTYLI
                     NSARELENFFDKKIRGLEVQYARSMRVSFQSDPNVLLRYVFRLRPDVGPLPVEHSFSL
                     GSLQYGRSASLLLEFLVEPLAQGAQEIHFMHGQIQLDIPSLTNRGGRILLDIKRPVLS
                     TIELEPPPPAVIDAISRLTLYRLQEKAHAEVAAGQITDAAQHLQHLATHLLAQGDRKL
                     AHTVLMEAEHIQQNHEFSEEGSKRVKYGTRALLMSPGSE"
     gene            11595..12104
                     /locus_tag="PKL11_01255"
     CDS             11595..12104
                     /locus_tag="PKL11_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNZ12252.1"
                     /translation="MIICPNCHHHELPGAMFCSECGAQINLADTLTTQSIRPSTADLP
                     GAPAEAAPIPPVSGMGDAIVSLHILEAGKVIPLEGRTEFTLGRAAEGQPILPDIDLSS
                     YDAYTLGVSRLHAAIKIIGQRVVMVDLGSSNGTRVNGQKIIPNGDFPLSHGDMLSLGK
                     LKIQVLIHY"
     gene            12118..12357
                     /locus_tag="PKL11_01260"
     CDS             12118..12357
                     /locus_tag="PKL11_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12253.1"
                     /translation="MPTLLVHVANEDPILGEVDDIPQASDVLIYIKNPRRRDGKDIHY
                     IEPAVTLIGIPFWRINFFEVMPSTEEEDIISFVRE"
     gene            12366..13079
                     /locus_tag="PKL11_01265"
     CDS             12366..13079
                     /locus_tag="PKL11_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ12254.1"
                     /translation="MDDSYRNRLILVVDDEERMARFIRLNLEHDGFRVVEAYRGLQAI
                     DRVRTELPDVVILDVMMPDMDGFEVLKLIRENGTVPVIMLTARGEEDDRIRGLELGAD
                     DYVTKPFSPRELVSRVRAVLRRSEMTAAPTSDVIVVDDRLKLDFGRREVWVAGELVKL
                     RPTEYRLLYHLVQNAGWVLTYDQLLTRVWGYEYRDEPHYVRLYINYLRQKIEEDAANP
                     KYILTERGVGYRFVDYRRM"
     gene            13141..13503
                     /gene="trxA"
                     /locus_tag="PKL11_01270"
     CDS             13141..13503
                     /gene="trxA"
                     /locus_tag="PKL11_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ12255.1"
                     /translation="MSEPIHVTDAEFDATVLKSTLPIIVDFWAPWCGPCRMVAPILDK
                     LAKDFDGKLTVAKVNTDENPEWATRFGVQGIPTMLFVFGGKVVHTQVGALPEAALRDI
                     VTQFLDFAASAANGPTPA"
     gene            complement(13588..14871)
                     /gene="murA"
                     /locus_tag="PKL11_01275"
     CDS             complement(13588..14871)
                     /gene="murA"
                     /locus_tag="PKL11_01275"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNZ12256.1"
                     /translation="MEKFIIEGQHPLHGEMTPAGNKNAALPLLAACLLTDEPVLMHNV
                     PAIGDVKAMRALLESLGVEITTVSNHTWQIHAREVRPADLDPDMCRKIRASILLAGPM
                     TARSGELHLPPPGGDVIGRRRVDTHLLALRKLGADVHYDRSFHFQAKDGLKGADMLLD
                     EASVTGTENAIMAAALAHGESIIRNAASEPHIQELCHFLNQLGARIENIGSNTLHIKG
                     VPRLHGGEFTIGPDYLEVVSFIGAAAVTHGSIRIRNAGPQYLDMIHLVFGRLGVTWNT
                     EGQDIVVPAEQEFVIEPDLGGMIPEISVMPWPAFPTDLMSMALVVATQSTGTILFHDW
                     MYPSRMFFIDKLVGMGAHIVLCDPHRAIVQGPSPLSGEKMESPDIRAGMSLVLAALSA
                     KGTSVIRNIGQIDRGYERVDEKLTALGAVIERTSD"
     gene            14937..15704
                     /locus_tag="PKL11_01280"
     CDS             14937..15704
                     /locus_tag="PKL11_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ12257.1"
                     /translation="MYLLDPNPSGSPCVLLLHGLGMDGSSWFLQTRALVAAGYRPLAV
                     DLPGFGQSPRSNGRWTMRRAVAELVAELTARGLPPIPLVGISLGGALALQIALDYPHI
                     PTRLVLINAFPCLRPLSREMSRYLLRRAFRSAFVGPRAQAEAVAAHLFPRAEQTFLRT
                     EVVSRILQTDQRTYRAAMRAIALLDLRPRLAELRLPVLVITGERDGTIPPRNQTQMAR
                     RIPGAVQVVIPDAGHGVVIEQPDRVNQELLAFLRPSV"
     gene            complement(15668..16420)
                     /gene="tatC"
                     /locus_tag="PKL11_01285"
     CDS             complement(15668..16420)
                     /gene="tatC"
                     /locus_tag="PKL11_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNZ12258.1"
                     /translation="MSRAANELTFLGHIEELRRRLLIAVGALIAATLVGLALTPQLVE
                     LLARPIGGTQNLQAIQVTENIAVFMKISLLGGVILASPVILYEILAFLSPALEKNEKR
                     ILAVGIPAATLLFLGGVAFTYFVMLPVAVPALFSTLDVPTTPTASNYFGFITNMLFWM
                     GIGFETPIVVFLLARLQIITPKMLLKNWRIATVVVAILAGVITPTVDPVNMALLMAPL
                     LLLYWISVLLAFLAMRKPKARQTEGRKKANNS"
     gene            complement(16417..16797)
                     /locus_tag="PKL11_01290"
     CDS             complement(16417..16797)
                     /locus_tag="PKL11_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12259.1"
                     /translation="MELLGLGLGEWALVLVILFVVMGPKEMMTLSRRIALGIKKIRQS
                     DLWRSIQETERELRQVPQELMKETGLDELEQERRKILANVRTGSPVVGWEPNPAEQSK
                     PMGWEPESDTSASMPESSGTRIKE"
     gene            complement(16821..17015)
                     /locus_tag="PKL11_01295"
     CDS             complement(16821..17015)
                     /locus_tag="PKL11_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNZ12260.1"
                     /translation="MYRFSGYEILIVLVIVIVLFGPERIAKYAKGFGKSISAFLNGLK
                     DKGEEQPEDDHKPHNGTSES"
     gene            17298..18206
                     /locus_tag="PKL11_01300"
     CDS             17298..18206
                     /locus_tag="PKL11_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNZ12261.1"
                     /translation="MKIGVVLMLMELPGLNRALTWNEIRQTALHNESLGFDSLWIYDH
                     LLYRSPKGQTTGIWEGWSILAALAAATQRAELGTLVACNSFRNPALLAKMAHTVDEIS
                     GGRLILGIGAGWNQPEYDAFGFPFDHRVDRFEEALQIIRPLLKEGQVDFSGKYYTARD
                     CEITPHSPRPGGPPLLIGAGKPRMLRLTAQYADLWNVGYMSVPRSTETEFKAFRRACK
                     AVGRDPATLPATFMVSLAYPDLLAWKSDKKRGYLTGSIEEIAVVLKEYETLGAAHLMF
                     HINPSTPQAYERLAKSVELYRTQASH"
     gene            complement(18238..19881)
                     /gene="pgi"
                     /locus_tag="PKL11_01305"
     CDS             complement(18238..19881)
                     /gene="pgi"
                     /locus_tag="PKL11_01305"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ12262.1"
                     /translation="MITQLTESPAWRALKDHQRIIGEMQMRDWFAEDSQRFERFSRRV
                     GEILFDFSKNRITEKTLMLLIELARQADLEQWIEAMFTGQKINSTENRAVLHVALRNR
                     SNRPIIVDGQDVMPEVNRVLGKMRRFSEAVRSGEWRGYTGKAITDIVNIGIGGSDLGP
                     KMVCEALKPYARADLRVHFVSNVDSTDLVETLKQVSPETTLFLVASKTFTTQETMANA
                     HSARQWFLNAAKDEAAIAKHFAAMSTNAAAVKQFGIDVDNMFEFWDWVGGRYSLWSAI
                     GLSIALYLGMDRFEELLNGAHKVDVHFRTTPFESNVPVIMALLGVWYNNFFNAHSHAI
                     LPYDQYLVHFPAYFQQGDMESNGKSVDRQGAAVNYATGPIIWGQPGTNGQHAFYQLIH
                     QGTQLIPCDFLAAAQSQNPLGEHHSILLSNFLAQTEALMRGKTADEVRKELSAGGTAG
                     EQLELLVAAKTFEGNRPTNSLLYPQLTPEVLGMLIALYEHKIFTQGVIWNINPYDQMG
                     VELGKQLAKKILPELTGAEQVNSHDSSTNGLINVIKAMR"
     gene            complement(19932..20612)
                     /locus_tag="PKL11_01310"
     CDS             complement(19932..20612)
                     /locus_tag="PKL11_01310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ12263.1"
                     /translation="MSTTRLYLIRHGATRLSAEDRFSGGTEVDLSDEGRWQAACLSKR
                     LADDPIVAFYCSPMRRTVETATIIAQPFHLIPEQREGLREIHHGHWETMSRMDVEAQY
                     PEEYAAWQEDPFTFAPEGGESGVMVMARALPVIREIVLKHAGQAVAVVSHKATIRLIL
                     SSLLGFDARGYRDRLDQSPACLNILDFKDPVRARLMLFNDVSHYVDQPPYARNRLSDW
                     FDGPSPTG"
     gene            complement(20626..22284)
                     /gene="pgm"
                     /locus_tag="PKL11_01315"
     CDS             complement(20626..22284)
                     /gene="pgm"
                     /locus_tag="PKL11_01315"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNZ12264.1"
                     /translation="MSTHPLAGKPAPYEVLINVPRLISAYYTYQPNPDDPAHRVAFGT
                     SGHRGSSLLHSFNEPHILAISQAICEYRQGQNITGPLYLGMDTHALSEPALSSAVEVF
                     AANGVEIVVQQGLGYTPTPVISHAILAHNRGRKTGLADGVVITPSHNPPGDGGFKYNP
                     PTGGPADTQTTKWIQDRANAILAGGLKAVKRTALEKALKASTTHLYDYIRPYVIDLEN
                     VVNLRAVAAAGLKIGVDPMGGAGVGFWGLIAEHYGLNLDVVNPRIDPTFAFMTLDHDG
                     KIRMDCSSPYAMASLVGMKDQYDIAFGNDTDYDRHGIVTRSVGLMNPNHYLATAIWYL
                     YQNRAGWRGDVAIGKTLVSSSMIDRVAAHLGRKLCEVPVGFKWFVDGLLDGSFGFGGE
                     ESAGASFLRVDGTVWTTDKDGFILDLLAAEILAVSGRDPGEHYQALEQQFGKAYYARR
                     DAPISPEGKTVLGNLSPEKVAASKLAGEKITAKLTQAPYNQAAIGGLKVVTENGWFAA
                     RPSGTENVYKIYAESFISPDHLGQIQTEAQAIVDAALKSSGEAA"
     gene            22416..24494
                     /gene="tkt"
                     /locus_tag="PKL11_01320"
     CDS             22416..24494
                     /gene="tkt"
                     /locus_tag="PKL11_01320"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNZ12265.1"
                     /translation="MGQAAGIIFGILKSMQWITTSEKTMSENKTLQDLCVNTIRFLSA
                     DGVQQAKSGHPGLPMGAAPMAYTIWTRHLRHNPSNPNWPDRDRFILSGGHGSMLLYAL
                     LHLTGYALSLDELKRFRQWGSLTPGHPEYGLTPGVETTTGPLGQGFANGVGMAMAEAQ
                     LASVFNRPGHEVINHYIYAIVTDGDLMEGVASEAASLAGTLKLGKLIYLYDDNRISIE
                     GSTDIAFTEDRGQRFEAYGWHVQRVDDGLDVAAIDRAIQAAKADPRPSLIMVRTVIGY
                     GLPTRAGTAKAHGEPPGDAELDGAKEQLGWPKEPRFHIPPESLAHFRQAVDQGARLEQ
                     QWHQKMTAYAAAFPELAAELNRRLSAQLPAGWAAALPEFPADPKGLASRATSGKVINA
                     LAPVLPELFGGSADLAPSNNTWMNDIPAFSPADYAGRNVHFGVREHGMGSVVNGLAYH
                     GGIIPYGATFMVFSDYMRPPVRLAALSHLGSIWIFTHDSIGVGEDGPTHQPVEQLAAL
                     RAIPNLITLRPADANEVAAAWKIAIENRRRPTALVLTRQNLPTLDRSIYAPADGLRRG
                     AYVLADLGGKQPQLILMASGSEVSLIIEAGNQLAAQSIGVRLVSFPSWELFAEQDQAY
                     RDQVLPPALESRLAVEAGVSQGWHRWVGSKGQIQALDRYGASAPANQVFENLGFSVAH
                     IVQQALDLIK"
     gene            24511..25617
                     /gene="tal"
                     /locus_tag="PKL11_01325"
     CDS             24511..25617
                     /gene="tal"
                     /locus_tag="PKL11_01325"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNZ12266.1"
                     /translation="MNTTLQLLASGQSVWYDNIQRSLLQNGEMAGMIQRGEIRGVTSN
                     PSIFMNAITKSADYDSSLLPLIRAGLNDEEIFFHLAIEDIQAAADLFLPLYQQTGGGD
                     GYVSLEVSPFLASDTAGTLAQAKALWQRVNRPNLMIKIPATPAGLPAITAAIAAGINV
                     NVTLIFSRQRYAEVMNAYLAGLEQRVADGLPLDKIASVASFFVSRVDTNIDARLQQII
                     AQGGASAAQAKLLLGQAAVANARLAYADYQQVFSAPRFQKLKARGARVQRPLWASTST
                     KNLAYRDVIYVEELIGPDTVNTVPPQTLVAFLEHGQIRPSLTENLPAAQQLFANLAAI
                     GISIDEVTLQLEVEGVKAFSDAFSVLLKAISDRR"
     gene            25647..27791
                     /locus_tag="PKL11_01330"
     CDS             25647..27791
                     /locus_tag="PKL11_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNZ12267.1"
                     /translation="MNPKQAIKLLNKSIGFGLLLAVLLSACQTPRPVSQPAGAPPAAS
                     IPTPLPAAVTSEITGRVVDLDGNPIDGAVVSSDTGSAQSDAQGWFIVPGSGTAQWLTV
                     THPAYISRTRAGAPGAPLLFRLTPDDGKTIVVHFVGDTMFGRRFFDPNEDGDTSDGLL
                     PTTPTLSDHAALLEPIQPLLANADLTVINMESPLSEQPYISPRDTRPQAFHETKEFVF
                     ASHPSSIPALQQAGVDVISLGNNHMYDLLETGLNDTLQTLDTAGLPHFGAGADETAAW
                     QPALISVSGQTLAFIGCTTISAPVPPVSDHDVTYTASDRLNKGGAAECEASALSNAIQ
                     QARASSDTVIVMIHGGYEYDRSPSPRVAEFTALARQAGADLIINHHPHVVGGLDWDGS
                     TLAANSLGNFIFDQTIWPTFESYLLAVTIRDGEVIRVYAEPLGVVDYVSRGLTGELAG
                     YVSREAAGLVPGPFVMENGAVEVDFRAQALRRVYTVALDDQGQPGQLIAIPENEWISQ
                     CVGSGSLRPGRDLLWVGGFENQSVDGSAAAPLFWMPGDSVQFGSQFAYAGLNGLRLSR
                     GSTNVQDVVTTHLHRVLVDGGSPLTVTGMLRASAGAILSLQISWYPDTLGPSASMTHL
                     LLNVTAPDTWEPFRLDVEAPPGTVAAGIYIRMSPPVSGTATADFDNLRLIEWAPEGTP
                     FSPLYDYAQLTGACEATYYEQVLPGAEFDLFR"
     gene            complement(27788..29824)
                     /locus_tag="PKL11_01335"
     CDS             complement(27788..29824)
                     /locus_tag="PKL11_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNZ12268.1"
                     /translation="MPNRLINETSPYLRQHTDNPVNWEPWDEAALQRARQENKPIFLS
                     IGYAACHWCHVMAHESFEDEGIAALLNEHFICIKVDREERPDLDSIYMNAVVAMTGQG
                     GWPMSVFLTPELEPFYGGTYFPPVRRYQMPAFGEVLTGIWQAWTEDRPNLLQAAKRLT
                     RHLREHAEWGTQSGVLEPESLSAAAEALINSQDGRNGGWGRAPKFPQPMAIAFLLGQA
                     ARGNARALDAAVDALRAMQRGGLMDLIGGGFHRYSTDERWLVPHFEKMLYDNAQLAAV
                     YLRGWQLAGEESFRQTCEATLDFMLREMQDPAGGFYSSLDADSEGGEGYFYTWSVEEL
                     RALLERHEWQVVEMLYGITERGNFEGRMILQAQMTQNAAAARLGMPPEQFYAALESAH
                     ARLRTARAKRSRPLTDDKVLVGWNALALRVFAEAAFPLKRADYLAAAQKNAAFLLSQM
                     FINGRLRRAWRAGEARHAGTLEDYAGLILGLVALYQADFNLQWLRAARVLTEKMQIDF
                     ADPNGGFYDTSAAAGELLLRPKDLPDNATPAGNALATQALIIMAALDERPDWFDQAAA
                     MLTGLQGSAAAHPLSFGCWLQGLDAAVGPLRQVAIVWQADGEGPDALLAAILNDYTHN
                     MIITASPYPPEKDAPALLQNRPPLNGQPTAYVCTGATCQLPVTEPDRLRMMISG"
     gene            complement(29829..30209)
                     /locus_tag="PKL11_01340"
     CDS             complement(29829..30209)
                     /locus_tag="PKL11_01340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNZ12269.1"
                     /translation="MEPFNQLKEAKFLNLETFRKNGNGVKTPVWFVQEGEVLYVRTIA
                     DSGKVKRIRNNQQVNVAPCKANGDLTGGWAPGKAHEINGDQALMGKVDRLLSKKYGLR
                     KVIFSVMWALQGKQYTLIEIKPGT"
     gene            complement(30228..32891)
                     /locus_tag="PKL11_01345"
     CDS             complement(30228..32891)
                     /locus_tag="PKL11_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNZ12270.1"
                     /translation="MNKLLGKHWQHLPPKEVIELLDSNIERGLDLFEVKHRQERFGLN
                     ALTPKKGKSPLMRFLMQFNNPLVIILVVASVVTAVLKDLTDALVIFGVVLINAVIGYI
                     QESRAEQAIAALAKTMTTEAAVVRGGKVVRLSAVELVPGDIVQLQAGARVPADLRLVT
                     SRDLQVTEAALTGESVPVEKDANLLIEPEAVLAERRNMAYASTLVTYGTATGVVVATG
                     DGSEIGRISQLISSAVELETPLTRKISQFSRVLLVAILILSAVTFGVGVLRGQPVVDT
                     LMGAIALAVGAIPEGLPAALTVTLAIGVSRMAKRRAIIRNLPAVETLGSTTVICSDKT
                     GTLTQNQMTVQELKTTKGTYEISGVGYQPEGTISGNAAEDAAVQEMLKAGVLCNDSQL
                     VETDGYWTIQGDPTEGALLVSARKAGLSLEAGDYPARLDSIPFDSQHQYMATLHADGM
                     IYVKGAAEVLLERCNGAMDASGNVITCGSDVFRSALEQMAARGLRVLAFARLEKPGAK
                     QITFEDITGLTLLGLQGMIDPPRPEVIDAIKASQRAGITVKMITGDHALTAKAIGEQI
                     GLCKDGCSTVLTGADLLRLSDAELMERVDQVNVFARVAPEQKLRLVEALQARGHVVAM
                     TGDGVNDAPALKQSNIGVAMGITGTDVSKEAADMVLTDDNFATIEAAIEEGRGVFDNL
                     TKFISWALPTNLGEGLVLLAAIITGATLPILPIQILWINMITAAVLGIALSLEPKEPG
                     IMERKPRNPKAPLLSKMVIWRIILVSIFILAGAFGLFEYELSRGASIEEARTVAVNVV
                     IFVELLYLFNARSLTLSPFQLGFFSNPWAIGGSVLMILIQLLYTYAPFMNTIFGSAPI
                     PFILWIDILAVSLGAYLIVEIEKWLRRKFSR"
     gene            complement(32888..33325)
                     /locus_tag="PKL11_01350"
     CDS             complement(32888..33325)
                     /locus_tag="PKL11_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12271.1"
                     /translation="MNYEHRFRVEAALSRVRDFHSRAESMAAITPPPVFIKMRSTPAT
                     LNENDIMDFTMWMGPLPIHWTARIENVTETGFSDRQIRGPFAHWLHRHTFAAVSEEAT
                     EVVDEITVKLSHDPLWWLIGIFMWVGLPFLFAYRGWKTKRMLK"
     gene            33434..33838
                     /locus_tag="PKL11_01355"
     CDS             33434..33838
                     /locus_tag="PKL11_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNZ12272.1"
                     /translation="MSNPAANRLYQILQRWGLALVLMLAIFLFSNTPSTEIPNLGGWD
                     RLVKKGGHMLGYALLALTLLRGFGSAGWRNILFAFIGAALYAASDELHQSFVPGRHAA
                     LLDVAIDSLGAALGLLAARHLPVLQKIIHRGL"
     gene            33924..34868
                     /locus_tag="PKL11_01360"
     CDS             33924..34868
                     /locus_tag="PKL11_01360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12273.1"
                     /translation="MKLTLRTYQSDDDYWRIRDFLRQVMVANHRREFSWSVARWDYWW
                     WFANPDLEKISLTENVFIWETPDGQIAAVLNPEGRGQVFLQRHPDFISPDLDAVMIAL
                     AEDRLTAPNQDDRRQLQIFVDSQDTAIHQTLTRRGFQRVERPGEQEYQHRRSLDLPLP
                     PPIKLPDYTIRPLGTGLEFLERCYASGLGFHDDDIQVARDNRDHPDWYRHIQSAPLYR
                     RDLDLVVIAPDGSVASFCTIWFDDVSRTAYFEPVATVPAHRQRGLGKAMMIEGLHCLK
                     QMGCKVAFVGGYSPGANALYFSVMGPEYDVSEPWEKTI"
     gene            34949..35623
                     /locus_tag="PKL11_01365"
     CDS             34949..35623
                     /locus_tag="PKL11_01365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ12274.1"
                     /translation="MIPIRDSIPTRRVPIVNYALIGANLLVFLLMWLAGPEQERLVYQ
                     FALIPASFSNGIDLGDITDIFTSMFMHAGLAHIAGNMLYLWIFGDNVEDRLGSFKYLL
                     FYLAGGLAASLTHLLFNPNSQIPTVGASGAIAAVLGAYLVMYPQSRIATFIPLGFFMR
                     LAMVPASVVLGFWFILQLFNGFLSIGAADVGGVAFWAHIGGFVAGVVLAKLMPAPRPS
                     ASVITW"
     gene            35773..36900
                     /locus_tag="PKL11_01370"
     CDS             35773..36900
                     /locus_tag="PKL11_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12275.1"
                     /translation="MVEIQKIDTTDRRQVNEFVNFHYRLYAGTPQWVPPFYNDIKLML
                     NREKHPFYEHSDADFFIARRGDEVVGRIAAMELKPFNQVHGVKDSSFYLFDTVDDIDV
                     AKALVTAAAEWAQKRGLTRVVGPKGFSPFDGYGIQIEGFEHHQMMTMMNYNFPYYQRL
                     MEELGFEKKVDFVSCYLHRDNFQLPEKAREIARRVQERGTFTVKNFASKAEMLKWGDR
                     IGEAYNKTFVNNWEYYPLTQREIKFTVDSVLTVAVPQFIKIILHHDEVVGFLFAFPDI
                     SQAIQRARGRLTPWSIADMLISLKRTNWVSGNGVGVLPEYQGRGGTALMYAELEKTVH
                     TPQFEHLEMTQVAESAVQMRRDLINLGGKAYKNHRVFQRSI"
     gene            complement(36950..38224)
                     /locus_tag="PKL11_01375"
     CDS             complement(36950..38224)
                     /locus_tag="PKL11_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12276.1"
                     /translation="MNKKKLLPLLTAGIILLICIPYWAGTLGVPFHPDESAQLYMSAD
                     VELLFENPTDLFWTPAETSGLREQYRLLDAPLTRTLIGLGRVLSNTTALPADWVWSAS
                     WEENSGNGALPDRRLLAVGRLSVAWLFPLTLWFTYEAGKSLGGKRLGCLALVLMTINA
                     LVLLHTRRAMAESALLFCVALFIWLALREKTPAWLLGLAAGLAFCAKHSAVTLLPLGL
                     VAVLWVRHSQLHWTRYLLRAGAYLLAFGLVYGLLNPWLWQYPVQATQAALTARQELAA
                     RQTAEMRAANADQALDTPGERLVGALAQVYFAPPAIWDVGNYAEVQQSDADHYLANPL
                     HQLLRSFGGGGILLALTLVGIALAVRGVIKEPTERRGLALLLLGFGLQLGFQAGFVTL
                     PFQRYVIPLLPWIGLLMGYALHKAIRLPMKRK"
     gene            38293..39915
                     /locus_tag="PKL11_01380"
     CDS             38293..39915
                     /locus_tag="PKL11_01380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ12277.1"
                     /translation="MKIWKRDIFWLGLILLVAAGLKLWLLLAERVPFNADEAVVALMA
                     RHILQGERPIFFYGQAYMGSLDAWLVAGGFAIFGQQVWVIRLLQTLLYLGTIATTYLI
                     GKTILGSSRTGLIAAALLAVPTVNVTLYTTASLGGYGEAILIGNLMLLAVHAIGQRLL
                     PDAPLAPRVWGGVVLWGFLAGLGLWSNALSLIYAIPSGLYLLWKLRWGSGWRRGWPII
                     PAALVGFLLGSLPWWLYAAQAGLGHLIGELTGSAVAVEKGSYALQILNHLFNLILLGG
                     TVTFGFRPPWEVRWLGLPLLPFVLMFWAVVIIFMARGIERKQEHWPERRMIVGVMVVF
                     WLAFILTSFGVDPSGRYFVPTAIPLALIAAETIQQVSQKFWIRLALIALVIGFHIYGT
                     IQCALRYPPGITTQFYEPSVVDHRYLPELIDFLKTEGETTGYTNYWVSYPLAFESDES
                     LIFVPRLPYHADLRYTERDDRYAPYDAVVMQSTRAAYITTRNAPLDEYLRAQFTALGI
                     TWQERQIGDYQVYYRLSVLVRPEQIGLGSSTP"
     gene            39912..41420
                     /locus_tag="PKL11_01385"
     CDS             39912..41420
                     /locus_tag="PKL11_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12278.1"
                     /translation="MTVTVKSSAQQPLERLILVLTLVLVFLMAARTPLESDLWWHLRA
                     GEETLQTGQPMLTDTLSFTRAGEHWINHSWLSEVVMALVYRAAGPLGLGLLVALLAVL
                     SMGLVYLQMEGPGFLRAFLIILASLVAALVWSPRPQMASLLGIAALGGILYLNKWRQR
                     DLLWLMLPLFLLWSNLHGGYSIGFLILAAALGGEILNHLLGFTGPEVLSWRRIARLAL
                     WTGACALVVLINPNGLDTWRIPFQTVGVQTLQQAISEWASPDFHDLAQQPFLWLLLAT
                     LASVGLSGRRLDGVDLLTVTGFAYLALVARRNFGPFALVAAPVLARHLWAALQSWPTR
                     LPAFQSWLNQLAERRARAKPAPPTLARVLNLLIVALLAFAAFIKLYIVTQPTFYGALV
                     YSTSPTAAVHWLQENRPTGPLLSEYGWGGYLTWHLPEYPVFVDGRTDLFGDEIIGDWL
                     TVVEARSGWPAVLSQWDVRLIMLEPGRPVISELDAAGWTRLYSDDIAVIYGR"
     gene            41410..42570
                     /locus_tag="PKL11_01390"
     CDS             41410..42570
                     /locus_tag="PKL11_01390"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNZ12279.1"
                     /translation="MDAEPHPIEIKSWPRFLVGGLMIARLLMLLALPLEGLRGYGDFQ
                     HFFNLAAIPGWPYLDHWVEFPPIFPFLIELLYRISGAQQHTFDYLLFFILTAADVGSL
                     LVFGRLLTHLNPPSALKRWLMYALALVALAYAWWYFDSLPVLALLLGLLWIIEGREWR
                     AGAVLGLGLITKLFPALALAAAWRLLPPRRALRVTLLTLGIGLIAYGALYLAAPQFTA
                     ASLISQGAKGSWETVWALLDGNLRTGSFGPLVERLEPALANVSSGQPARIPTWIPLGL
                     AAALGFWRFLKARRTPLAALALTGLAWCLLMLASPGWSVQWTLYALPFMLLLLPDKEA
                     PLLAVTFTLVNLLEWPILLSRGLFWAMPLTIILRALLLVLLAIRFDQRARQP"
     gene            complement(42561..>43348)
                     /locus_tag="PKL11_01395"
     CDS             complement(42561..>43348)
                     /locus_tag="PKL11_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HNZ12280.1"
                     /translation="TMIFTLSLAQFKIEIGRPAQNIAKAKWLIAEAARAGSGLILFPE
                     LWTSGYDLANASRYAAENAELLPTLAKISREEQIAIGGSYLTERQGKYFNSFILLRPD
                     GQPPATYDKLHLFRLMQEEQWLGAGEAGQTARFDWGEAGLSVCYDLRFPELYRRYALD
                     GAQAAWVVAEWPVRRITHWQTLLRARAIENQMFVAGVNAVGEIGEAVYGGHSAIITPW
                     GEALIEGNGKDEALLTADLDFELVAQARRTIPVFEDRRKDIYG"
BASE COUNT         8705 a        13686 c        10936 g        10021 t
ORIGIN      
        1 ggttccggtt tgcattcccc ctccaattcg agtaaaatag acaaatctta tcaagaatta
       61 ggaggcgcat cctttggcaa agcgaaccgc aaaacccaag tccaaaccca ggccagaaac
      121 caattccaac ctctggctct ggctcattct aggcggtgct gtcctggcgt tggtcatagt
      181 tgcaatcaca gccaacccgc gccaaccatc tgccgcagcc accattctgc ccccgaccgt
      241 tgtgcttccc acacttacca gtagctcacc cgcggcccct atcactcctg cagtcacaac
      301 cgaaatccct actgttgtgg ctgctgcccc cgaccctgcc gtgctggtca cccctgaccc
      361 cacctggccc tatcctgaaa tcccccgcgt cacgctggaa ggcgcccggg cagcatttga
      421 ttccggcgct gccgtgattg tggacacccg ttccgctgaa tcctttgcac agagccatgc
      481 cgtgggtgcc atcaatatcc cggttaacga gatggaagcc ctctataccc agcttgaccc
      541 caaccagtgg atcatcccct actgtacctg agggcacgaa aatgcaagcg ccggtctggc
      601 gcgattcctg ctggataaag gttttaccaa tgtcaccccg ctgctgggcg gttacacggc
      661 ctggctaaac gccggttacc ccaccgaacc ttaatcactg ccaatcaaaa gcccggctct
      721 tggccgggct ttttttattt tcctaaaggt cgaagtacat ttgaatttca tacggatgtg
      781 ggcgagaccg cacctgcagg tcttcggccc gcttgccctt cacccaggtc cgcagcattt
      841 cctcagtgaa cacgccgcct tccagcagaa aatcataatc ggcttccagt gcatccagtg
      901 cctcttccag cgaggtcggc aggctcttga ttgtagcccg tttttccgct ggccacgaga
      961 agatgtcatc atcaatcggg ccaaatccag ccggggttgg gtcaatcttg cgccggatcc
     1021 cgtccaggcc agccatcaat tgtgctgcca tcgccaggta ggggttgcag gtcgcatcgg
     1081 gggggcggaa ctcaaagcgt accgtgtctg gctgggtggc atacttgggc acccggatgg
     1141 ccgcgctgcg gttaccgctg gagaagaacg cgctgatcgg tgcctcaaat ccgggcacca
     1201 gacggcggta tgagttggtg ctgggattgg taaacgccag tacggccggg gcgtgggtca
     1261 gcagcccgcc gatgtaataa agcgctgtct tgctcagcag gttgggcccc ttggcatcat
     1321 agaacaggtt cgccccgtcc ctcagcagtt gctgatggaa gtgcatcccg ctgcccgcct
     1381 cgccatacag cggcttgggc aggaaggtga ccgtttgccc cgccgcattg gcaaccattt
     1441 tgctgatgta tttgatgacc agcgaagcat ccgctgccgc cagcaaaccc atcaaaggcg
     1501 tctcaatttc gcactgacca ggcccgccca cctcgtgatg atgatatttg accggtacgc
     1561 caacctgctc cagcgtcatc accattcggg cgcgcaggtc ataggtctgg tcgcggggcg
     1621 gaatggcatg gtaacccccg tgcagcggca gcaaatgtcc atcaccgccc tgaacgctgt
     1681 tccattgccc ttcgtacgca tccacccggt agctggctgc attgacagtg ttttccaaca
     1741 ccacatcgtt gaaaacatag aactcaaatt ccggccccca caggctttcg gttgcaaagc
     1801 catgttcctg catataggcc tcggcccgtt gaaccacaat gcgcggatct ccggcaaagc
     1861 gttctttggt gtctgcctcc accgtgtcgc agatgaagct cagcgtgggc atttcccaga
     1921 agggatccaa aaatccggtc gtcaggtccg ggattaatgc catgtcaccg gccttaacac
     1981 tcttgaagcc gacactggag ccgtcaaagc caattccggc ttccatcaat tcgggcgtga
     2041 actcactggc agaaatggta acatggtgcc agcgacccca taaatcgcaa aacttaaggt
     2101 caatcatgcg aatatcattt tcgcgcacga acgttgccgc ctcaataaag tcctggaaca
     2161 tggtttctcc tcttgaattt gttttatttt tgaagggctg catgcagcct tacttctttt
     2221 tccgtgtcaa cgcccagatg cccagccctg ccagcgccgc cacagccgcc gtcgcggccg
     2281 ccgcggtgat gaccgtcttg ggctccatcc ccggcatcac aacttcgccg tccccataac
     2341 tgcggaacag cctcccccat tgggtttgca gcgcttcttc cacgcgcctt cgtcctacga
     2401 acgggtacca gtacacgttg tgatagaagt tgctcgcaaa gaaactccac ggcaccagcg
     2461 gcgattgcag caaaggcttt tccagcggct tgagcggccc gtgataaatc atcttctgcc
     2521 cgcgggatgc aaaagtgtct tcctgtttga agtgccaggc aggttcctgt tcaatatcgt
     2581 aacccacaat ctcaatctcg cggggatcgc ccacacccaa ccccatctcg tgcgccatgc
     2641 ggataaacgg gatagacatt gggtcgaacc cctgcagctt ggctgagatc gcatcaattg
     2701 ctacctgatc cgccgatgcg agcaagatgt ttttctcgtg ccagcgcatt gcacgcggcc
     2761 ccggcccatc cccggcaaag gttccgtcca tcaccgcaaa aatccccgga tgaatttcct
     2821 gctgaatgcg cagcaaatcc accagggttt cgtgaatcac gctgtgtgtc cagtgtcggt
     2881 tgcggtgcag tagtccacca aaagcattct tcattgctcc ggtaatggtc gtgaagacat
     2941 gggtcttaac cgtgggcaga tgcacaatgt tccgcccaat cagggctttt gggatgaaaa
     3001 caccctccgg aaaaacccga tccaatacca ggtaatcgcc gtgccgcagc tcatactgtt
     3061 gccactcaaa ctgctgctca tataagtaca tgcagggcac gttgtattta tcggttacaa
     3121 accgatgctt gttattggct tcgccgtctc gcgtattgac caccaccgta tcattgtgga
     3181 tgcccataat gtcggggtac cccaatcgct gcagggtgcg gatggtgcct tccaactgcc
     3241 agggcgtgct ggagcaggcc gggtaccagg tctgccatga gatgttaatt ttgatgcccg
     3301 tcggcacgtt tttcggcagc gcctgttcca cgccagccat caccatcagg cggtcaatat
     3361 cttccagcac cgtttccggt gatgttttca gaacggcaac ttttccttta ccagggtaag
     3421 tgctcatgct atttctccat tagaattaca acttaaacag tctgataatg attgtaacgg
     3481 aatttggagg caaagtccag ttacactcta cccgttccgg tgggacgctt ctcccaattc
     3541 tgcgcgaatg cgccccgcca tttgccgggc atgggcgggc gccagcccca ggtaaccctg
     3601 aacttcgcac aatgggcgca gttcctccac cttcagccag cgggcaatca gatcatcgta
     3661 catcagcaat gtcagcagcg ggttgggctg cccctgctga accgctgccc aggcttgcaa
     3721 tgcatgctgc cggatgcgct catgcatttc ctggcggtcg gcgccgcgtt ttgccactgc
     3781 catcagcacc cgttctgtgg ctgcaaatgg cgcatacatt tccaggttgc gttgaatagc
     3841 cgcttcattg ataaccaatc ccttgatcat tttttccgcg gttattaaca gttcatcgca
     3901 ggtcaaaaac gcttctggca gcagtgtccg gcggttggcg ctgtcgtcca gcgtccgctc
     3961 cagcagagag tgcgccgcat tatgccaggc cgtcaccggc atctgtgcca gcgtccgtgc
     4021 cagcgaatca atcttttccg cctggatggg attccgcttg aagggcattg ccgacgatcc
     4081 cacctgcttt ttgccaaatg gctcgctcca ttcgcccagc ggcggcgatt gcagcagccg
     4141 taaatcaaaa gcaaatttat ataaggattg ccccagtccc gctagcgcac acaacagtgt
     4201 atactcctgc ttgcgtgggc tggtctgggt ggtcaccggg taaaaaggca gcttcagccg
     4261 ggcgctcagg ctggcttcaa agggtcccag ccgttccact cccaccagtt caccgtaggc
     4321 cgcgcccgtt cccaccgccc ccttgaatcc ctttccccga atctggttcc gccgccccac
     4381 cagcgcatga taatcttcca ataaatcttg cgcataggta gatagccggt agcccagtgt
     4441 ggtgggctct gctggttgca ggtgtgtgaa cgccatcacc ggcaagtctg cctggcgctc
     4501 cacctgttcc gccaggctca acagcagcgc ttgcagccgc ggcagcagca aatccagcgc
     4561 ctgccggatg cgcaaagcat cggcgttgtc ctcaatatcc atagaggttg cccccatgtg
     4621 caggctgccg ccgccagtgg ggcactgctc ggcaaaagtg cgcagctccg ccatcaggtc
     4681 atggtggatc tcactctcga tttccagtgc ccgttcaata tcaatatcat caacatgcgc
     4741 ctggatatcc gccagcgcat tcgtcggaat caggttgaat tcagcttcgg tttctgccag
     4801 tgccagccag atgcgtcgcc agatgcgccg cttttcctgc tcactccaca ggcgccgcat
     4861 ctctgcgctt ccataccgaa aactgaatgg cgaacgatag gtctcaaatt ccataaaatc
     4921 ctcaccggtg tcgttttctt ctattttact tgaattgcgt tgtttataca tctttcgagc
     4981 cggttttatg aatagcatta ccgcaccgtt cgcccgaaaa ttagatcctc acggcacccc
     5041 ccacagccga acatcgccgc ttgccgcacc cgatgccaat atcatgccat cggctgagaa
     5101 tgccaccccc gtgatgctgg aggtatgggc ccgcagccgc aaaatttcag tcgctgtgtt
     5161 tacatcccag agccgtaggt tgtcttcact ggcacagccg gcagccagca ggctcccatc
     5221 aggcgagaag gcaaggctgt taaccgattt catatcgccg ccaagctgac ctagtgaatt
     5281 gccgctgttg gcatcccata ggtcaatctt cccatccgcc ccgccgcttg ccagcatctt
     5341 gccgtctggt gaaaaggcca ggctcagcac gccctcggcg ggtccggtca accggcgcat
     5401 ctcttctccg ctcaccgggc tccacagaat gatgctgcca tcgctgccgc ctgccgccag
     5461 ggtgctgcca tctggcgcaa aagccacgct ggagacggct cccatccctc cgttcaactc
     5521 catgaggccc gctccgttgg ccgcgtccca taaccgcacg gtgccatcct tgctgcccga
     5581 tgccagcagg ctgccatcgg gtgaaaacgc cagactgtta atcagatatt catgcccttc
     5641 cagcgtacgc agctcagccc cgctctgcgc atcccacagt ttgatgatcg cgtcattgcc
     5701 cccggaagcc aaaatacctc catctggcga aaatgccgcg ccacgggttt gcacggtgtg
     5761 gccttctagc ctcatcagcg gggtgccgct cggcaccgcc cagacttgca tgctgccatc
     5821 gcctgccccg gctgccagtt ttgtgccatc cggcgcaaag gccagactga aaataaaatt
     5881 gatatgtccc gatgcctgct gcagttcggc gccgctggcg acatcccaca gatgcagcac
     5941 tccatcataa ctgtaagaaa aaatggtttg accatcaggc gcatattctg ctccaaaaac
     6001 cttgctccca tgttccaacc tgccaaccgc ctgcaggctg cctgcttcca aaatgtgaac
     6061 gctgccatca aaacatccac ctaccagggt tttcccgtcc ggcgcataag caatggacat
     6121 ggtgagtcct aaggattgct cctccagctt ggcggtttca accccactct ccacatccca
     6181 caggtgaaat gcctgactgc cagccgaagc cagggttttt ccatctggcg aaaaatcaac
     6241 gctgtaaacc ggtgcgttcc catggtccag gctgtgcaat gctgttgcat tgtttacatc
     6301 ccatataata gcgatatgat ccaacccagc agaggccagc cgcgtcccat caggtgaaaa
     6361 aaccaacccg tgaatttgct gtgtgtgtcc ttctaattgc cgcagttcag cccgggtgct
     6421 cacatcccat aggtgtatca ccccatccgc cccgcttgat gccagcactt tcccatccgg
     6481 tgacaatgcc agataggttg ccgttccgcc cgagccctca aattgcccca cctcagctcc
     6541 gctctgagca tcccagacct caatttccga cccgcccgaa aatagttgat tgccatcggg
     6601 cgagtacgcc actgcagtga tatagctgtg ggtgtcaata aacatccgca tctctaaatt
     6661 ctgccgatca taaatataga tgccgtaacc tgccccaacc gccacccggc tgccatccgg
     6721 cgaccaggcg ctgctggtaa tattgccctt gcccagcacg ttgatcagag tcaactgcgc
     6781 tccattctcc gggctgatga cctgattcgc ctcaggtcgg ggcgcttcag tgggtggcag
     6841 cgccgtcgga tactccaccg gcgccgcagt ttgttcctcg ccgggttgcg gcgtggactg
     6901 ggcttcgggc tgtgcagttg ccagtgctcc cggcgccggc gtacaggccg ccaggactaa
     6961 actgaatcca atgaacattg ctaaccagac ggtcaaatgc ttattcatgt cagcctccct
     7021 caaacagact tgtccatgtc caaaattgga tgagattgat gcaaatatta tatgcccgat
     7081 taaaaaacgc aacccaatta ttgccctgta ctttcatccc cccgcttgtt gaagaacccg
     7141 ctctcatcac cgcgtagatt tgcataatca tgtataataa tcattagttg cagccagatc
     7201 ccgtgccagc agctcaggca tcattattta gcaattatct ttttccgggc tggcgggggc
     7261 aagtggctgg cttctgcctg gaggatcatc tggacactca caacatcacc cagaacttga
     7321 accaggggca aagtccccca ccgaagcagc ttcaacccgg ctacgttctt ctggaccgct
     7381 acctcatcca ggaagttatt ggcgtaggcg gcatgggctc cgtctaccgc gcccgcgact
     7441 tgcacttcca aaacgtcatc aaacttgtcg ccgtcaagga gatgatcaac tccagccccg
     7501 accccatcgt gcgccagacg attgtccaga actttgagcg cgaggccaat atccttgccg
     7561 cgctcaatca cccctccatc cccaaaattt ttgacttctt ctcccacgac gagcgttctt
     7621 acctcgtcct ggagtttgtc cacggcaaag acctcgaagc cctgctcaac gagcgggatg
     7681 actttttccc cgaagagcag atcatcggct gggcggtgga gctttgtgac gtactgcaat
     7741 atctgcacct gcacaaaccc gagcccatca tcttccgaga catgaagccc tccaacgtca
     7801 tggtcaatca acagggccac atcgtcctgg tggattttgg aattgccaag tcattccgca
     7861 ccggtcaaaa aggcaccatg atcggcactg agggttactc gccgccggaa cagtaccggg
     7921 gtgaagccac ccctctggct gatatttatg ctctgggcgc caccctgcac catgccctca
     7981 cgcgccgcga cccccgcctg gagccgccct tctcctttgc cgagcgtccc gtccgcgcct
     8041 caaatccagg cgtctcacag gagcttgaaa caatcgtcac cacggcgttg cgctacaacc
     8101 ccgatgaacg ctttccttcc gccgaggcga tgaaagatgc cttgttgacc gtcgcacgca
     8161 agaccggcgt actctccaaa atttccatgc cctccactga ccggtcatct accaccatta
     8221 aaccgctttg gaccttcaaa tgcgaagatg aaattcgcgg ctcggcggtt tttgaaaatg
     8281 gcgtggtcta tatcggctct tacgataata acctctacgc ggtcaatgcc ggtaatggtc
     8341 aatttatctg gaaatacccc gccaacggcg gcattgtggg ccgccctgtg ctttctgagg
     8401 gcaatctcta ttttggctct gaagataatt cgctgtatgt catctcatcc cgcactgggc
     8461 gcatcgtctc cagcttccaa acccagggcc ccatccgttc ctcgcctcgc atcgccgaag
     8521 gccatatttt cttcggttca gatgatggct ttttatacgc catcaaccag accagcaccc
     8581 gttcctcctg gcgttttgag gtgggtgaac cggtccgttc cacaccagcc gcatcccagg
     8641 atgccatctt ctttggcacc gagggcggcg aatttttatc agtagatttt cgcgggcagg
     8701 cgcgctggcg cttccgcgcc aagcgagcgg tcacctcatc gcctgtggtc acccagggca
     8761 cggtctattt tgcctcggtg gattcgtccg tctatgccct cgacattcgc tccggctgga
     8821 tgttatggcg cttccgcatg gaaaaaggct ctgtctcctc cccctgccgt gccgaaaata
     8881 tgctttttgt cggttctgcc gatggtaaca tctactgcct ggatgccggc aatgccaaag
     8941 aaatctggca tttcaagacc gggcatcagg tcagcggctc gcctgccatc tatcgagact
     9001 cagtctactg cggctcagcc gatggcaaca tctactgcct ggaataccgc accgggcgtc
     9061 tgcgctggcg cttcactacc ggcgggccca tcaccggcac accggtgatt gttgatgatg
     9121 tgctctacat tggctcagcc gatcacttgc tctatgccct gctggcctga cgcagtcatt
     9181 ctgttggatt aagaggtaat gcgtgatcca attttttaaa aacctttttc ctcgcaaaaa
     9241 atctgaggcc ctgccatctg ccaaagacat gatccagact gcccccctgg acgcagaatc
     9301 tgaggatcct gccgcggggc gctctcccta tcgcccggcc cagttcctgg tcggttgcgc
     9361 ccaatccgtt ggcatccagc gggatcataa cgaagatacc ctctttgcca tgtcctctgt
     9421 tctcgccgat ggcgtcaatg acatcccctt tggcattttt atcatcgccg acggtatggg
     9481 cgggcatcaa tatggcgaaa ttgccagcgg tgtcgctgcc cgaaccatgg ctaaccatct
     9541 cctggcgcgg gtctatcagc ccctgaccaa ccccgacctg gagcgctcca atgacagcct
     9601 gcaggaactg atggaaaccg gcgtccgtga agcacatcag attgtcttgc gcaaagcccc
     9661 cggtggcggc acaaccctca cggctgccct cctgctgggc gagcaaatca ccatcgccca
     9721 tgtgggtgac agccgcgcct atttcatcta ttccgatggc cgcatccgcc ccctaaccca
     9781 ggatcattcc ctggtgcagc gtctggtaga attaggccag ttaaccgcag aacaggccgt
     9841 caatcacccg aacaaaaatg tgctgtaccg cgtcttgggg caaaccgaac ccttccgccc
     9901 cgacatcgaa aaccgcatga tgccccaccc cggctatctg atggtctgca gcgacggttt
     9961 atggggcgtc ttgcccgaag atgacttgct taagattgtt cagcagtcat ccaacccaac
    10021 cgaggcttgc cacaaactgg tcgaagccgc caatgccgcc ggtgggccag acaatatcag
    10081 cgtgatcctg gttcaattct tatagctagg ggagcagatg ccatcaggtg cagattacta
    10141 taacgctttg gggctgacca ttgatgccac acccgaggag attcggacgg cttactttga
    10201 ggctgcccgc cgcctgcacc ctgatatcaa ccccgacccc aaagcacagg aaatttttct
    10261 tcaaattcaa acggcttatg atgtcctttc aaatccccgc cgccgggcag attatgatgc
    10321 tggcttgcca gcggatttgc gcgcccgtcc cgatattaac ctgagcgtaa aatacagccg
    10381 cccggctgtc ccggccattg gcgaagcgca gcttttttat gtccttttgg atctgaactg
    10441 caccgccgag ttggatgcaa cccgcacccc gccggtcaac ctctgcctgg tcattgaccg
    10501 ctccacctcc atgcgcggcg aacgcatgga catggtcaaa gccaacgttg ttcaattggt
    10561 tcgccagctt cgcccgcatg atattctctc cctggtgctg ttcagtgacc gcgccgaggt
    10621 ctttctgcct cccatgcggg tggccgatat caaccgcctt gaaagtcaga tcagcatggt
    10681 gcagtgtggt ggcgcaaccg aaatcttccg cggcctcgag gctggctata gcctgctgac
    10741 gcgctacggt gattctcgcg cgaaccggca cctggttttg ttaaccgatg gccatactta
    10801 tggcgacgaa gcccccgccc tcaacctggc tgaacgcgcc gcccaggaaa atatcgccac
    10861 cagcgccctc ggcattggca gcgagtggaa cgatgttttc ctcgaccgtc tgaccagttt
    10921 ttcgggcggc aacacctacc tgattaattc ggcgcgcgag ttggaaaact ttttcgacaa
    10981 gaaaattcgc gggctcgagg ttcaatatgc ccgcagcatg agagtgagct tccaatcaga
    11041 tcccaacgta ctgctgcgtt atgtctttcg gctgcgcccc gatgttggcc cgctgcctgt
    11101 agagcacagc ttttccctcg gatccttgca atacggccgc agcgccagcc tgctgttgga
    11161 gtttctggtt gaaccattgg cgcaaggcgc ccaggaaatc cacttcatgc atggccagat
    11221 ccagttggat atcccttctc tcaccaaccg tgggggccgc atcttactgg atatcaagcg
    11281 cccggtgctt tcaaccatcg aattggaacc cccaccccct gccgtcattg atgcgatttc
    11341 ccgcctcacc ctctaccgcc tgcaggaaaa agcccatgcc gaggttgcgg ctggccaaat
    11401 taccgatgcc gcccagcacc tacagcatct cgccacccat ctgttggccc agggcgatcg
    11461 caaattggcc catacggtct taatggaagc agagcatatc cagcagaatc atgaatttag
    11521 cgaggaaggc agcaagcgcg tcaagtacgg cacccgcgcc ctgctcatgt cgcccggatc
    11581 ggagtaatgg aattatgatt atttgcccca actgtcatca tcacgaactg cccggtgcta
    11641 tgttttgcag cgagtgtggc gctcaaatca acctggcaga cacactcaca acccagtcca
    11701 tccgcccttc tacggcagat ctccctggcg ctccggctga agccgccccc atcccgcctg
    11761 tctccggcat gggtgatgcc atcgtctcgc tgcatatcct cgaagccggc aaggtgatcc
    11821 cgcttgaagg ccgcaccgag ttcaccctgg ggcgagccgc cgaaggccag cccattctgc
    11881 ccgatattga cctttcatcc tatgacgcct acaccctggg ggtttcccgg ctgcacgctg
    11941 ctattaaaat catcggccag cgagttgtca tggtggacct gggctcttca aacggcaccc
    12001 gggtcaatgg acaaaaaatc atccccaatg gcgattttcc cctcagccat ggcgacatgc
    12061 tgtcgctggg caaactcaag atccaagtct tgatccatta ttgataggag gccagctatg
    12121 ccaacccttt tagttcacgt ggcaaacgaa gaccccatct taggcgaagt ggatgatatc
    12181 ccccaggcct ctgacgtgct tatctacatc aaaaaccccc gccgtcgtga cggcaaagat
    12241 atccactata ttgaacctgc cgtcaccctc atcggcatcc ctttctggcg tattaacttc
    12301 tttgaagtca tgcccagcac tgaagaagaa gatatcatca gctttgttcg ggagtagtaa
    12361 ccgtggtgga tgacagctat cggaaccgtt tgattctggt tgtggatgac gaagagcgca
    12421 tggcgcgctt catccgcctc aacctggagc acgatggctt ccgggtggtt gaggcttatc
    12481 gcggcctgca ggccattgac cgcgtccgca cagaattacc cgatgtggtg attctggatg
    12541 tcatgatgcc cgacatggat ggctttgaag tcctcaaact gatccgtgaa aacggcaccg
    12601 ttccggttat catgctcacc gcccgcggcg aagaagatga ccgcattcgc ggcctggagt
    12661 tgggcgccga tgattacgtc accaagcctt tcagtccgcg cgaactggtc agccgggtgc
    12721 gtgccgtgct gcgccgcagc gaaatgaccg ccgcccccac ctccgatgtc atcgttgtcg
    12781 atgatcgtct caagctcgac tttgggcgcc gtgaagtctg ggttgctggc gaactggtca
    12841 aacttcgccc caccgaatat cgcctgctct accacttggt gcaaaacgct ggttgggtgc
    12901 tgacgtatga tcagctcctg acccgtgtct ggggttatga atatcgcgac gagccgcatt
    12961 acgtccggtt atacatcaat tacctgcgcc agaagattga agaagacgct gccaatccca
    13021 agtacatcct cacagaacgg ggtgttggtt accgttttgt ggattatcgc cgcatgtaac
    13081 ttttgcatgt ttggcgcatc tatactatct gaattcaaaa gctaatgagg agatccgcta
    13141 atgtccgaac ccattcatgt aaccgatgct gaatttgacg ccacagtatt aaaatccacc
    13201 ctgcccatca ttgtcgactt ttgggccccc tggtgcggcc cctgccgcat ggtagccccc
    13261 atcctggata aactggcgaa agattttgac ggaaaactga ccgtcgccaa agtcaatacc
    13321 gatgaaaacc ctgaatgggc cacccgcttt ggcgttcagg gcatccccac catgttgttt
    13381 gtctttggtg gcaaagtggt ccatacccag gtcggcgccc tccccgaagc cgccctgcgc
    13441 gatatcgtca cccagtttct cgattttgcc gccagcgccg ccaacggccc cactcctgcc
    13501 tgagattgat cgcttaattc ccaaaaacag taaccgggcg gcttgcaaaa aaccgcccgg
    13561 ttttattgtt tctgttaatc tacagtgtta atcgcttgtg cgctcaatca ctgcgcccag
    13621 cgcagtcagc ttttcgtcca ctcgctcgta cccgcggtca atctgaccaa tattgcggat
    13681 caccgacgtt cccttggccg aaagcgccgc cagcaccagc gacatccccg cccgaatatc
    13741 cgggctttcc attttctccc ccgagagcgg gctgggtccc tgcacaatcg cccggtgcgg
    13801 atcgcatagt acaatgtgtg cgcccatccc caccagctta tcgatgaaga acatccggct
    13861 ggggtacatc caatcgtgga acagaatcgt ccccgttgat tgggttgcca ccaccagcgc
    13921 catgctcatc aaatcggtcg ggaaggccgg ccacggcatc acgctgattt ccgggatcat
    13981 cccgcccagg tccggctcaa tcacaaattc ctgttctgcc ggcaccacaa tatcctgtcc
    14041 ctcggtattc caggtcaccc ccagccgccc aaacaccaga tggatcatat ccaggtactg
    14101 cggtccggca ttgcggatgc ggatactccc atgtgtgacc gccgccgccc caataaagct
    14161 gactacttcc aggtaatctg gcccaatggt gaactcgccc ccgtgcagcc gcggcacacc
    14221 cttgatatgc agggtattgg acccaatatt ctcaatcctg gcccccagtt gattaagaaa
    14281 atggcacagt tcctgaatat gaggctctga ggccgcatta cggatgatcg actcgccgtg
    14341 agccagcgct gctgccatga tcgcattttc ggttcccgtc acgctggctt catcaagcag
    14401 catatcggcg cctttcaatc catccttcgc ctggaagtgg aaagagcggt catagtgcac
    14461 gtcggcgcct agttttcgca gcgccaacag gtgcgtatcc acccggcggc ggccaatcac
    14521 atcgccgccc ggcggcggca ggtgcagctc gcccgagcgg gccgtcatcg gcccggccag
    14581 caaaatcgaa gcccggatct tgcggcacat atccgggtcc agatcagccg ggcgcacctc
    14641 gcgggcatgg atctgccagg tatgattgct gacggtggta atttctacgc ccaggctttc
    14701 cagcagggcg cgcatcgctt tcacatcccc aattgccggc acgttatgca tcagcaccgg
    14761 ttcgtcggtc agaaggcagg ccgccagcag cggcagcgcc gcattcttgt ttccggctgg
    14821 cgtcatctcc ccgtgcaggg gatgttgacc ttcgatgata aatttttcca tagcaacctc
    14881 cgtgttcagg gtattataat gataaatcat cattttgtgt ttttccggag gtggcgatgt
    14941 atctcctgga ccccaaccca tccggcagtc cctgcgttct gcttctgcac ggccttggca
    15001 tggatggcag ctcctggttt ctgcaaaccc gggcgcttgt cgctgccggt taccgcccgc
    15061 tggcagttga tttgcccggt tttgggcagt ctccccgctc aaatggtcgt tggaccatgc
    15121 gtcgcgccgt ggctgaactg gtcgccgagt tgaccgctcg cggtctgccg cccataccgc
    15181 tggtgggcat ctctctgggc ggtgccctgg cgcttcaaat tgccctcgac tatccccaca
    15241 tccccacccg cctggtcttg attaatgcct ttccctgcct gcgcccgctg agccgtgaga
    15301 tgtctcgtta cctgctgcgc cgggccttcc gttctgcttt tgttggccct cgggctcagg
    15361 ctgaggccgt tgctgcccac ctctttcccc gcgccgagca aaccttcctg cgaaccgagg
    15421 tggtttcccg catcctgcaa accgatcaac gcacctaccg cgccgccatg cgcgccattg
    15481 ccctgctgga cctgcgcccg cggttggcag aactgcgctt gcccgttttg gtcatcacag
    15541 gtgaacgcga tggcaccatc ccaccgcgca atcaaaccca gatggctcgt cgcatccccg
    15601 gcgctgttca ggttgtcatt ccagatgccg gtcatggcgt cgtcatagaa cagcctgacc
    15661 gggtaaatca ggagttgttg gccttcttgc gcccctcggt ctgacgcgcc ttcggcttgc
    15721 gcatcgccag aaaagccagc agcacgctga tccagtaaag cagcagcagc ggcgccatca
    15781 ataaagccat attgaccggg tccacggtcg gtgtaatcac ccccgccagg atagccacca
    15841 ccaccgtggc aatgcgccag ttcttcaaca gcattttcgg tgtaatgatt tgcagccgcg
    15901 ccagtaaaaa caccacaatc ggggtttcaa acccaatccc catccaaaag agcatattgg
    15961 taataaatcc aaaatagttg gaggccgtgg gtgtggtcgg tacatccagt gtgctgaata
    16021 aggcgggtac ggctacaggc agcatcacaa aataggtgaa cgccaccccg cccagaaaga
    16081 gcagcgtagc cgccggaatg ccaacagcca aaatccgctt ttcgtttttc tccagcgcgg
    16141 gcgataaaaa cgccagtatc tcatagagga tgactggtga cgccagaatc accccgccca
    16201 gcagagatat cttcataaag acggcgatat tctcagtcac ctgaatggct tgcaggttct
    16261 gggttccgcc aatcggtcga gccagtaact caaccagttg gggcgtcagc gccagcccga
    16321 ccagtgtggc agcaatcagc gcccccacgg caatcagcag ccgccggcgc agttcttcaa
    16381 tgtgccccaa aaaggtcaat tcgttggccg cccggctcat tcttttattc gcgttcctga
    16441 tgattcgggc atagacgcgc tggtatctga ctcgggctcc catcccatcg gctttgactg
    16501 ttcagccggg ttgggctccc agccgacaac cgggctgcct gttcgcacat tcgctaaaat
    16561 cttgcggcgt tcctgttcca attcatccag cccggtctcc ttcatgagtt cctggggcac
    16621 ctgccgcagt tcgcgctcgg tttcctgaat ggatcgccac aaatccgact gccggatttt
    16681 ttttatcccc agcgcaatcc gccgtgacag ggtcatcatt tctttgggcc ccatcaccac
    16741 aaacagaatc accagcacca gtgcccattc acccaggccc agacctaaga gttccatttg
    16801 ttttgctcaa caataataga ttagctttca gatgtgccgt tatgcggctt gtgatcatct
    16861 tcgggctgtt cctcgccctt atctttcagt ccgttcagaa aggctgaaat gcttttacca
    16921 aaccccttgg catatttagc aattcgttcc gggccaaaca gcacaatcac aatcaccaga
    16981 acgataagaa tctcgtatcc tgaaaagcgg tacatgtcaa actccttata ggtttatgat
    17041 tataaccgat tgcttttcaa tctgagagat gaaaggtctg aaatttgtat aattttggta
    17101 tacatttatt aaatttcgtg gttttctgct ttcaaccatc ccgatgcaat aaagatgact
    17161 ataatatctg tactgcaaag cgcagcgaat tccaatgcaa gttagctggc catatcttga
    17221 actgtagatt tttgactgtc gtcccagttc gaggctaacc ggttcctgag ggatcaaatt
    17281 tttggaggtt caacgtgatg aaaattggtg tcgttctcat gctgatggaa ttgcctggcc
    17341 tcaaccgggc cctgacctgg aatgaaattc gccagaccgc tctgcacaac gaatcactcg
    17401 gctttgactc cctctggatt tacgaccact tgctctaccg ctcacccaaa gggcaaacca
    17461 ccggcatctg ggaaggctgg agcatactcg ctgcgctggc cgccgccacc cagcgggcgg
    17521 aactggggac gctggtcgcc tgcaactcgt tccgcaaccc ggccctgctg gctaaaatgg
    17581 cccacaccgt ggacgaaatc agcggcgggc gattgatcct gggcattggc gccggttgga
    17641 accagcccga atacgatgct tttggcttcc cctttgacca tcgagttgac cgctttgaag
    17701 aagcattgca aatcatccgc ccgctgctca aagagggcca ggtggatttt tccggcaagt
    17761 attacaccgc ccgcgattgt gaaattaccc ctcactcgcc gcgcccgggc ggcccgcccc
    17821 tgttgattgg tgctggcaaa ccgcgcatgc tgcgcctgac cgcccagtac gccgacttgt
    17881 ggaacgtcgg gtacatgtct gtcccccgct ctaccgaaac agagttcaag gccttccgcc
    17941 gggcatgcaa ggccgttgga cgtgacccgg ccaccctgcc tgcgaccttc atggtcagcc
    18001 tggcctaccc cgatttgctt gcttggaagt ctgacaaaaa gcgcggctat ctcaccggtt
    18061 ccattgagga aattgctgtt gtgctgaaag aatatgaaac cctgggcgcc gcccacttga
    18121 tgttccatat caacccttca acgcctcagg cctacgagcg cctggcaaaa tcggtggagt
    18181 tgtaccgtac ccaggcgtca cactgatcgc gcgtagctgt gctgtcagcc caccagttta
    18241 ccgcatggct ttaatgacgt taatcaatcc attggtggat gaatcgtggc tgttcacctg
    18301 ttctgctccg gtcagctctg gcagaatctt tttggcaagc tgcttgccaa gttccacgcc
    18361 catctgatcg tagggattga tattccagat cacaccctgg gtgaagatct tatgctcata
    18421 cagcgcgatc aacatgccca gcacttccgg cgtcaattgc ggatacagca gtgaattagt
    18481 cgggcgattg ccctcaaagg ttttggctgc caccagcagt tccagttgct ctcctgctgt
    18541 gcctccagcg cttagttctt tccgtacctc atccgctgtc ttgccgcgca tcaaggcctc
    18601 ggtttgggcc agaaaattgg acaacaaaat ggaatgatgc tcacccagcg gattctggct
    18661 ctgcgctgcc gccagaaaat cacatgggat taactgcgtg ccctggtgaa tgagctgata
    18721 aaatgcatgc tgtccattgg tccccggctg cccccagatg atcggcccgg tggcgtagtt
    18781 caccgccgca ccttgccggt ccaccgattt gccgttgctt tccatgtcgc cctgctggaa
    18841 gtaagccgga aaatgtacta gatactggtc atagggcagg atggcgtgtg agtgcgcgtt
    18901 gaaaaaattg ttataccaca cgcccagcag tgccataatc accggtacat ttgattcaaa
    18961 aggcgtcgtc cgaaaatgca catccacctt gtgtgcccca ttcaagagtt cctcaaaccg
    19021 gtccatgccc agatacagcg caatcgagag tccaatcgcg gaccacagcg aataccgccc
    19081 ccccacccaa tcccagaact cgaacatatt gtccacatca atcccaaact gcttcaccgc
    19141 cgccgcattg gtagacatcg ccgcaaagtg cttggcaata gccgcctcat ctttagcggc
    19201 attcaaaaac cattgccgtg ccgaatgtgc attcgccatg gtctcctgcg tggtaaaagt
    19261 tttggatgcc accagaaaca gcgtggtttc agggctgacc tgtttcagtg tctcaactaa
    19321 gtcggtactg tccacgttgg atacaaaatg cacccgcagg tccgcccgcg catagggctt
    19381 gagcgcttca cagaccatct taggccccag gtctgacccc ccaatcccaa tattgacgat
    19441 atcagtgatc gccttgcccg tgtatccccg ccattctcct gagcgcaccg cctcactgaa
    19501 ccgccgcatt ttgcccagca cacgattgac ttccggcatc acatcctgcc catccacaat
    19561 aattgggcgg ttggatcggt tccgcagcgc cacatgcagc acagcgcgat tctcggtgga
    19621 attgatcttc tgtccggtga acatggcttc aatccactgc tccaggtcag cctggcgcgc
    19681 cagctcaatt agcagcatca atgtcttttc tgtaatccga tttttagaga agtcaaatag
    19741 aatctccccc acccgccgtg aaaatcgttc aaaccgctgc gagtcttctg caaaccagtc
    19801 ccgcatctgc atctcgccga taatgcgctg gtggtctttg agtgcgcgcc aggctggtga
    19861 ttcagtcaat tgggtaatca taatatgctc cttacgatga aatgatggca tgaatgtatc
    19921 ttaccgtggc cttaccccgt aggcgatggt ccgtcaaacc agtcagagag ccggttgcgg
    19981 gcgtagggcg gctggtccac ataatgcgaa acatcattga acagcatcag ccgtgcccgt
    20041 accgggtctt taaagtccag gatattcagg caggccgggg attgatccag tcgatctcgg
    20101 tatcctcgcg cgtcaaatcc caacaggctg ctcaaaatca agcgaatggt ggctttatgg
    20161 gataccaccg ccacagcttg ccctgcatgc ttgagcacaa tttcccgaat cactggcagc
    20221 gctcgcgcca tcaccatcac ccccgattcg cccccctctg gcgcaaatgt aaagggatct
    20281 tcctgccatg cggcatattc ttcgggatat tgcgcttcaa catccatccg gctcatggtc
    20341 tcccagtgcc cgtgatgaat ctcccgcagt ccctcacgtt gctcgggaat caggtgaaag
    20401 ggttgggcaa tgatggtcgc ggtttcaacc gtccggcgca tggggctgca gtaaaatgcc
    20461 acaatcggat catcggccag ccgtttagat aggcaggccg cctgccatcg cccttcgtcc
    20521 gaaaggtcaa cttcggttcc ccccgaaaat cgatcttccg cactcaaccg cgtggcgccg
    20581 tgccggatta gataaagtcg ggttgtactc atggcgtctg acctttcagg ctgcttcgcc
    20641 actggatttc agcgccgcgt ccacaatcgc ttgcgcctcg gtctgtatct gccccaaatg
    20701 gtccgggctg ataaaactct cagcataaat tttgtacacg ttttctgtgc ccgaaggccg
    20761 ggctgcaaac caaccgtttt cggttaccac tttaagcccg ccaatggctg cctggttata
    20821 aggcgcctgt gtcagtttgg ccgtaatctt ctcgcctgcc agcttgctgg ccgcgacctt
    20881 ctccggtgag aggttgccca gcaccgtttt cccctctggg ctgatgggcg catcccgccg
    20941 ggcataataa gcctttccaa actgctgctc cagcgcctga taatgctcgc ccgggtcccg
    21001 cccgctgacc gccagaatct ccgccgccag cagatccaga ataaatccat ccttgtcggt
    21061 tgtccagacc gtgccatcca cccgcaggaa ggacgccccc gcgctttctt ctcccccaaa
    21121 gccaaacgac ccatccagca gcccgtccac aaaccacttg aagcctaccg gcacctcgca
    21181 gagtttgcgc cccaggtgcg ctgccacccg gtcaatcatc gaactggaaa ccagcgtctt
    21241 gccgatagcc acatccccgc gccaacccgc ccggttctga tacaggtacc agatggctgt
    21301 tgccaggtaa tgattgggat tcatcaaacc cacgcttcga gtcacaatgc cgtgccggtc
    21361 atagtcagtg tcgttgccaa aggctatgtc atactggtct ttcatcccca ccagactggc
    21421 catggcatac ggcgaagaac aatccatccg gattttcccg tcatgatcca gcgtcataaa
    21481 tgcaaaagtc ggatctatcc tggggttcac cacgtccagg ttgagcccat aatgttccgc
    21541 aatcagcccc cagaatccca cccccgcgcc gcccatcggg tcaacgccaa tcttcaatcc
    21601 cgcggctgcc accgcgcgca gatttaccac attttccagg tctatcacat acggtcggat
    21661 gtaatcataa agatgggttg tggatgcctt cagcgctttt tccagcgcgg tgcgttttac
    21721 cgccttcaac ccgccagcta aaatcgcatt tgcccggtct tgaatccatt tcgtcgtctg
    21781 cgtatccgcc gggccgccgg tcggcgggtt gtatttgaaa ccgccatcgc ccggtgggtt
    21841 atgagaaggg gtaatcacca ctccatctgc cagccctgtt ttgcggcccc ggttatgcgc
    21901 caaaatggca tgtgaaatca ccggagtggg ggtgtacccc agcccctgct ggaccacaat
    21961 ttcgaccccg ttggcggcaa acacctcaac cgcactggat agtgccggtt cggagagggc
    22021 gtgcgtgtcc atcccaagat acagcggccc cgtgatattc tgcccctggc ggtattcaca
    22081 aatagcctga ctgattgcca gaatgtgtgg ctcattaaag ctatgcagga gggatgatcc
    22141 gcggtgtccc gaagttccaa atgccacccg atgcgccggg tcatcgggat tgggctggta
    22201 ggtgtagtaa gccgaaatca gccgcggaac attgatcaac acctcatacg gggctggttt
    22261 gccagccagc ggatgtgtac tcatgggttc ctcctgctca aatggacagc ttcatggaat
    22321 gattggttat accctgattg tgctcgtttt tgtgaatttt gacaatgccc aaatcgcctc
    22381 tattttaagc ccgagaaccc ccatttgagg gtacaatggg gcaagctgca gggattattt
    22441 ttggtatcct caaatcaatg cagtggatta caacaagcga gaagaccatg tctgaaaaca
    22501 aaacccttca agatctctgt gtgaatacca tccgctttct ttcagccgat ggcgttcagc
    22561 aggccaaatc tggccatccg ggcttgccca tgggcgccgc tcccatggct tacaccatct
    22621 ggactcgtca cctgcgccat aatccctcca accccaattg gcctgatcgc gatcgcttca
    22681 tcctctccgg cgggcacggc tccatgctgc tgtacgcctt gctgcacctc accggctatg
    22741 ccctctcact ggacgaactc aagcgcttcc ggcagtgggg tagtctcacc cccggtcatc
    22801 ccgaatacgg cctgaccccc ggcgttgaaa ctaccactgg cccgctgggt cagggctttg
    22861 ctaatggcgt tggaatggcc atggctgaag cccagcttgc cagcgtcttc aaccgccccg
    22921 gtcatgaggt gatcaatcat tatatctacg ccattgtcac cgatggcgat ctgatggaag
    22981 gcgtcgcctc tgaggccgct tcgctggcgg gtaccctgaa gctgggcaaa ctgatctacc
    23041 tctacgacga caaccgaatt tcaatcgaag gctcaaccga cattgccttt accgaagatc
    23101 gcggccagcg ttttgaggcc tatggctggc acgttcagcg tgtggacgat ggtctggacg
    23161 tcgccgccat tgaccgggcc atccaggccg ctaaggctga ccctcgcccc tccttgatta
    23221 tggtgcgcac ggtcattggc tatggtttgc ccactcgcgc cggcacggcc aaagctcatg
    23281 gcgagccgcc aggtgatgcc gaactggacg gcgccaaaga acagttaggc tggccgaaag
    23341 agccgcgctt ccacattccc cccgaatccc tggcgcattt tcgccaggct gtcgatcagg
    23401 gtgcccggtt ggagcagcag tggcatcaaa aaatgacagc ttacgccgcc gcctttccgg
    23461 aactggctgc cgagctgaac cgccgcctgt ctgcgcagct tcccgcgggc tgggcagcag
    23521 ccctgcccga attccccgcc gatcccaagg gcctggcttc gcgggccaca tccggcaagg
    23581 tgatcaatgc cctggcgccc gtcttgccag aactgttcgg cggttctgcc gacctggctc
    23641 cctccaacaa tacctggatg aatgacattc cggccttttc tcctgccgac tatgccgggc
    23701 gcaatgtcca ctttggcgtg cgggaacatg gcatggggtc tgttgtcaat ggcctggcct
    23761 atcatggcgg catcatcccg tatggcgcaa cctttatggt cttttcagat tacatgcgcc
    23821 cgcccgtgcg cctggcggcc ctttcgcatc ttggctctat ttggatcttc acgcacgaca
    23881 gcatcggggt aggggaagat ggccccaccc atcaacccgt ggagcagctt gccgccctgc
    23941 gtgccattcc caacctcatc accctccgcc cggcagatgc caacgaagtt gccgctgcct
    24001 ggaagatcgc catcgaaaac cgccgccgcc ccaccgccct ggtgctcacc cgccagaatc
    24061 tgcccaccct cgatcgctca atctacgccc cggcagatgg tcttcgccgc ggggcctatg
    24121 tgttggcaga cctgggcggt aaacagccgc agctcattct catggcctcc ggctccgagg
    24181 tctccctgat tatcgaagcg ggcaatcagc ttgctgctca gagtattggg gtgcgcctgg
    24241 tctccttccc cagttgggaa ctgtttgccg aacaggatca agcctaccga gaccaggttc
    24301 ttcccccggc tctggaatcc cgcctggccg tcgaggctgg cgtttcacag ggctggcacc
    24361 gctgggttgg gtctaaaggc cagattcagg ccctggatcg ttacggcgcc tcggccccgg
    24421 ccaatcaggt ctttgaaaat ctgggctttt ctgtggccca tatcgttcag caggctctgg
    24481 atttaatcaa ataaccactc gggagtaatg atgaatacca ccttacaact gctcgcatcc
    24541 ggtcaatccg tctggtacga taacattcag cgctctctgc tgcaaaatgg tgagatggct
    24601 ggcatgatcc agcgcggcga aatccgcggc gtgacctcca acccctccat tttcatgaac
    24661 gccatcacca aatctgccga ttacgattcc agcctgctgc cactaatccg cgccggcctc
    24721 aacgatgaag aaatcttctt ccacctcgcc attgaggata tccaggctgc cgcagatctc
    24781 ttcctgccac tctatcaaca aaccggcggc ggtgatggct atgtcagtct ggaagtcagt
    24841 cccttcctgg caagtgatac cgccggcacc ctggcccagg ccaaagcctt atggcagcgt
    24901 gtcaaccgcc ccaacctgat gatcaaaatc cccgccacgc ctgccggcct ccccgccatt
    24961 acagccgcca tcgctgctgg catcaacgtc aatgtaaccc tgatcttctc acgccagcgc
    25021 tatgctgaag tgatgaatgc ctacctcgcc gggctggaac agcgcgtggc tgatggcttg
    25081 ccgctggata aaatcgcctc cgtggcctcc ttctttgtct cccgtgtaga taccaatatc
    25141 gacgcccggc ttcaacaaat cattgctcaa ggcggcgctt ccgcggccca ggccaagttg
    25201 cttctggggc aggcggccgt tgccaatgcc cgcctggcct atgccgacta ccagcaggtc
    25261 ttctcagcgc ctcgcttcca aaagctcaag gcgcgggggg ctcgggtaca gcgcccgctc
    25321 tgggcttcca ccagcaccaa aaatctggcc tatcgcgatg ttatctacgt cgaagagttg
    25381 attggccccg atacggtcaa taccgtcccg cctcaaaccc tggtcgcctt cctggagcat
    25441 ggccaaatcc gccccagcct cacagaaaac ctccccgctg ctcagcagct ctttgccaac
    25501 cttgccgcca ttggcatttc cattgacgaa gtcaccctgc agttggaagt tgagggcgtc
    25561 aaagccttct cggacgcttt ctctgtcctg ttgaaagcga tctctgaccg gcgctaatcc
    25621 actataaatc actggagggg aagaacatga acccaaaaca ggctataaaa ctgctgaaca
    25681 aaagcatcgg ttttgggctt ctgttggccg tgcttttgtc tgcctgccag actcctcggc
    25741 cagtatctca gccggcaggt gcaccgcccg ctgcctccat cccaaccccg ctgcctgccg
    25801 ccgtgacctc cgaaatcacg ggccgggtgg ttgacctgga tggcaaccca attgatggcg
    25861 ccgtggtgtc atccgacacg ggcagcgccc agagtgatgc tcagggctgg ttcatcgtgc
    25921 ctggcagtgg aacggcgcaa tggcttacgg taacccaccc ggcctatatt tcacgcacgc
    25981 gtgcgggcgc cccgggcgcc ccgcttctct tccgcctgac gcccgatgat ggcaaaacca
    26041 tcgttgtcca ctttgtcggc gataccatgt ttggccgccg tttcttcgat cctaacgagg
    26101 atggcgacac ctctgatggc ctgctgccaa ccaccccgac cctcagcgat catgcggctt
    26161 tgcttgagcc catccagccg ctgctggcca acgcagattt gaccgtgatt aacatggaga
    26221 gcccgctgtc agagcagccc tatatctctc cgcgagatac ccgcccccag gccttccacg
    26281 agaccaagga atttgtgttt gccagccacc catcatccat tccagcgctt caacaggctg
    26341 gcgtagatgt gatttcactg ggcaataacc acatgtatga tctgcttgaa acaggattga
    26401 acgataccct ccagaccctg gatactgccg gattgcccca cttcggcgca ggcgcagatg
    26461 agaccgccgc ctggcagcct gccctgattt ctgtgtctgg tcaaaccctg gccttcattg
    26521 gctgcaccac catttccgct ccggttccgc ccgtatctga ccatgatgtg acctacaccg
    26581 cctcagaccg gctgaataaa ggtggtgcgg cagagtgtga ggccagcgcc ctgagcaatg
    26641 caatccagca ggctcgcgca agcagtgata cggtaatcgt gatgattcac ggcggctatg
    26701 aatacgatcg ctcaccctcg ccccgggtcg ctgaattcac cgccctggcc cgacaggccg
    26761 gagccgacct gattatcaac caccacccgc atgtcgtcgg cgggttggat tgggatggct
    26821 ccaccctggc cgctaacagc ctgggaaatt tcatcttcga ccagaccatt tggcccacct
    26881 ttgaatccta cttgttagcc gtcacaattc gagacggtga agtaatccgg gtatatgccg
    26941 agcctctggg cgtggtggac tatgtttcgc gcggcctgac cggtgaactg gccggctatg
    27001 tctcgcgcga ggcggccggg ttggtccccg gtccttttgt tatggaaaac ggcgctgttg
    27061 aggtggattt ccgcgcgcag gccctgcggc gggtttatac cgtcgccctg gatgatcagg
    27121 gccaaccggg acagttgatt gccatccctg aaaatgaatg gatctcgcag tgtgtaggct
    27181 cgggttcttt gcgcccgggc cgcgacttac tctgggttgg cgggtttgaa aatcagtcgg
    27241 tagatggttc agccgccgcc ccgcttttct ggatgcccgg cgactcggtg cagtttggca
    27301 gtcagtttgc ctatgccggt cttaacggtc tgcggctgtc ccgcggcagt accaatgttc
    27361 aagatgtcgt cacaacccac ctgcaccgtg ttctcgtaga tggcggcagt ccattgactg
    27421 tgaccggcat gttgcgagcc agcgccggtg caatcctatc gctgcaaatt agctggtatc
    27481 ccgacacact gggcccgtca gcctccatga cccatctcct gttgaatgtt actgcgcccg
    27541 atacctggga gccctttcga ttggatgtgg aagcgccccc tggtactgtg gcggctggca
    27601 tctatatccg catgtcgcct ccggtcagtg gtacagcaac tgccgacttt gacaatctgc
    27661 gcctgattga atgggcgcct gaaggcacgc cgttcagccc gctctatgac tatgcccaac
    27721 tgaccggtgc ctgtgaagcc acttactatg aacaggtctt gcccggcgca gaatttgatc
    27781 tgtttcgtta acccgaaatc atcatgcgca agcggtctgg ttcagtcacc ggcagctggc
    27841 aggtcgcgcc agtgcagaca taggctgtgg gttgcccatt caatggggga cggttctgta
    27901 acagcgccgg ggcatctttt tcaggcgggt agggggatgc tgttataatc atgttgtgcg
    27961 tatagtcgtt tagaatggct gccagcagag catccgggcc ctcaccatcc gcctgccaga
    28021 caatcgctac ctgccgcagt ggccccaccg ccgcatccag gccctgcagc cagcacccaa
    28081 aactcaacgg atgtgctgcc gccgagcctt gcagccccgt cagcatcgcc gcagcctgat
    28141 caaaccaatc tgggcgttca tccagggctg ccatgatgat taacgcctgt gtcgccagcg
    28201 cattgccggc gggggtggcg ttatccggca ggtctttagg gcgcagcaga agttctcctg
    28261 ccgcggccga agtatcataa aatccgccat ttgggtctgc aaaatcaatc tgcatctttt
    28321 cggtcagcac ccgcgcggct ctcagccatt gcaagttgaa atctgcctga tatagcgcca
    28381 ccagtcctaa aatcaatccg gcgtaatctt ccagtgtccc ggcatggcgg gcttcgccag
    28441 cgcgccaggc gcggcgcagc cgcccgttga tgaacatctg ggaaagcaga aatgccgcgt
    28501 tcttttgagc cgccgccaga taatctgccc gcttcaacgg aaaggctgcc tcagcaaaaa
    28561 ctcgcagcgc cagcgcattc catccgacca ggactttatc atccgtcaat gggcggctgc
    28621 gcttggctcg ggcagttcgc agcctggcat gggcgctttc cagcgctgca taaaactgtt
    28681 ccgggggcat gcccagccgg gctgcggcag cgttttgcgt catttgcgcc tgtaaaatca
    28741 tccgtccctc aaaattgccc cgctctgtga tgccgtacag catctctacc acttgccatt
    28801 catgccgctc aagcaaagcg cgcagctctt ctacgctcca ggtgtagaaa taaccctcgc
    28861 cgccctccga gtccgcgtcc aggctgctgt aaaacccgcc ggccggatcc tgcatttccc
    28921 gcagcataaa atccagtgtt gcctcacaag tttgccgaaa gctttcctcg cccgccagtt
    28981 gccagccccg caaatagacc gcggccagtt gtgcattgtc gtagagcatc ttctcaaaat
    29041 gcggcaccag ccagcgctca tctgtgctgt accggtgaaa tccaccccca atgaggtcca
    29101 tcaacccgcc gcgctgcatc gcccgcagcg catccaccgc cgcgtccagc gcccgggcat
    29161 tgccccgcgc cgcctggccc agcagaaatg caatcgccat cggctgtgga aacttgggcg
    29221 cccgtcccca gccgccgttt cgtccatcct gtgaattgat cagcgcctct gccgccgcgc
    29281 tcaggctttc gggttcaagc actccagact gtgtgcccca ttcagcgtgt tccctcaagt
    29341 gtcgggtcag ccgcttggcg gcttgcagca ggttggggcg atcctctgtc caggcctgcc
    29401 agatgcccgt cagcacttcg ccaaacgccg gcatctgata ccgccgcacc ggcgggaaat
    29461 aagtgccccc gtaaaatggc tccagttccg gggtcagaaa cacactcatc ggccacccgc
    29521 cctgccccgt catcgccacc accgcattca tgtagatgct gtcgaggtct gggcgttcct
    29581 cccggtccac tttgatgcag ataaaatgtt cgttcagcag tgcggcgatg ccctcatcct
    29641 caaaggactc gtgcgccatc acatggcacc agtgacaggc tgcataccca atgctcaaaa
    29701 agatgggctt gttttcctgt cgggcgcgct gcagcgccgc ctcatcccac ggctcccagt
    29761 ttaccgggtt atcagtgtgc tgccgcagat agggcgaggt ctcgttgatt aatcgattgg
    29821 gcatgttgct acgtgccagg cttgatttca attagcgtgt actgcttgcc ttgcagcgcc
    29881 cacatcacgg aaaaaataac cttcctcaaa ccatactttt tgctcaacag ccggtcaact
    29941 ttgcccatca aggcctgatc gccattgatt tcatgtgcct tacccggggc ccagccgccg
    30001 gtcaaatcac cattggcttt gcacggagca acattgacct gctggttatt ccgaatccgc
    30061 ttgaccttac ccgaatcggc aattgtgcgc acataaagca cttcgccctc ttgcacaaac
    30121 catactggcg ttttcacacc gttcccattt tttcggaagg tttccaggtt taaaaacttt
    30181 gcttctttga gttgattgaa tggttccatc ttttttcctt gtcctcgtta gcgtgaaaat
    30241 ttacgccgca gccacttctc aatttcaaca atcaagtaag ctcccaggct cacggctaaa
    30301 atatcaatcc atagtatgaa tggaatgggc gcgctgccga aaatagtgtt cataaacggc
    30361 gcatacgtgt agagcagttg gattaagatc atcaagacgc tgccgccaat tgcccagggg
    30421 ttggagaaga agcccagctg gaagggggaa agcgttagcg agcgggcatt gaatagatag
    30481 agcagttcta caaagatcac cacattcacc gccaccgtcc gggcttcttc aatcgaagcg
    30541 ccgcgcgaca gttcatattc aaacaaccca aatgcacctg ccagaataaa aatgctcacc
    30601 aggataatcc gccagatgac cattttgctc agcagcgggg ccttggggtt ccggggtttt
    30661 ctttccataa tccccggttc tttgggctct aaagaaagcg caatgcccaa taccgccgct
    30721 gtaatcatat taatccacag aatttgaatg ggcagaatgg gcagcgtggc cccggtaata
    30781 attgctgcca gcagaaccag cccttcaccc aggttggttg gcagtgccca cgaaatgaac
    30841 ttggtcaggt tgtcaaaaac cccgcgccct tcttcaatgg ctgcttcaat cgtagcaaaa
    30901 ttatcatcag tcagaaccat gtcggctgct tcttttgaga catcggtgcc ggtaatgccc
    30961 attgccaccc caatattgga ctgcttaagc gctggggcat cgttaacccc gtcgccggtc
    31021 atcgccacca cgtgaccgcg cgcctgcagg gcctcaacca gccgcaattt ttgctccgga
    31081 gcaacccggg caaacacatt cacctgatca acccgttcca tcaactcggc atccgaaagc
    31141 ctcagcaaat cggcgccggt caatacggtg ctgcacccat ctttgcaaag cccaatttgt
    31201 tctccaattg cttttgccgt cagtgcatga tccccggtga tcattttcac tgtaattccg
    31261 gctcgctggc tggctttgat ggcatcaatc acttccgggc ggggcggatc aatcatgccc
    31321 tgcagcccca gcagcgtcaa cccggttata tcttcaaagg tgatctgctt tgcccccggt
    31381 ttttccagcc gggcaaaagc cagcacgcgc aaaccccgcg ccgccatctg ctccaatgct
    31441 gaccggaaaa catcgctgcc gcaggtgata acgtttccag aagcatccat tgcaccgttg
    31501 cagcgttcta acaatacttc agccgcgcct ttcacgtaaa tcattccgtc cgcatgcagt
    31561 gtcgccatat attggtgctg cgaatcaaac ggaatcgaat ccaatcgggc gggataatcc
    31621 ccggcttcca ggctcaggcc cgctttgcgg gcagatacca gcagcgcccc ctcagtagga
    31681 tcgccttgta tggtccaata gccatcggtt tcaaccagtt ggctgtcgtt gcacaacaca
    31741 ccggctttga gcatttcttg cacagccgca tcttcagccg cattgcccga aatcgtcccc
    31801 tccggctgat acccaacccc gctgatttca tacgtccctt tggtggtctt cagctcctga
    31861 accgtcatct gattctgcgt cagcgtgccg gtcttgtcgg agcagatcac cgtggtggac
    31921 cccagtgtct caactgctgg caggttgcgg atgatggcgc ggcgtttcgc catcctcgaa
    31981 accccaattg ccagggtcac tgttaatgct gccggcagtc cttccggaat cgcccctacc
    32041 gccagcgcaa tggcccccat caaagtatcc accaccggct gccctcgcag cacacctacc
    32101 ccaaacgtaa ctgccgataa tatgaggatg gcaaccaaca acacccggct gaattgactg
    32161 atcttgcggg tgagtggcgt ttccaactct accgcgctgg atataagctg cgaaatccgc
    32221 ccaatttcgc tgccatcgcc ggtagccacc accacgcccg ttgccgtgcc atatgtcacc
    32281 agcgtagaag cataggccat attccgccgc tcggccagta ccgcttccgg ctcaatcagc
    32341 aggttggcgt ctttttccac cggaaccgat tcgccggtca gggcggcttc ggtcacctgt
    32401 agatcgcggg atgtcaccaa ccgcaaatcg gctggcaccc gtgccccggc ctgcagttga
    32461 acaatatcac ccggcaccag ctcaactgcc gagaggcgca ccaccttgcc cccgcgcaca
    32521 acagccgcct cagtcgtcat ggtctttgcc agcgccgcaa tggcctgctc tgcccgtgat
    32581 tcttgaatat acccaatcac agcattgatc agcaccaccc caaagatcac cagggcgtcg
    32641 gtcaaatcct tcaaaacggc cgtcaccaca ctggcaacca ccaggataat caccagcgga
    32701 ttgttgaatt gcatcaggaa tcgcatcaaa gggcttttcc cctttttagg cgttaatgca
    32761 ttcagcccaa agcgctcctg ccggtgcttg acctcaaaga gatccagccc gcgctcaata
    32821 ttgctgtcca gcagttcaat cacttctttt ggcggcaaat gctgccagtg ctttcccagg
    32881 agtttattca tttcaacatc ctttttgttt tccagccccg ataggcaaat aagaacggca
    32941 atcccaccca catgaatatc cctatcagcc accatagggg atcatgggat agcttcactg
    33001 taatttcatc cacaacctca gtggcttctt cgctcaccgc agcaaatgta tggcggtgca
    33061 gccagtgtgc aaaaggccct cggatctgcc ggtcactgaa tcccgtttct gtcacattct
    33121 caatccgggc agtccagtga atcggcagcg gccccatcca cattgtaaaa tccatgatat
    33181 cattttcgtt cagggttgcc ggggtgctcc tcattttgat aaatactggc ggcggtgtga
    33241 tggcagccat gctttctgcc cggctgtgaa aatccctcac ccggctcagt gcagcttcaa
    33301 cccgaaaacg atgttcataa ttcatatcaa aatatcctat caagaaatat acgcttttat
    33361 gttgcctccc gtcaagtatt tctgtccgct aaccctctac taaaccattc tgcccggccc
    33421 ctgttaggaa cgcatgtcaa atcctgctgc caaccgcctc taccagattt tacagcgctg
    33481 gggcctggcc cttgtgctca tgctggcaat ctttcttttc tccaacacac cctccacaga
    33541 aattcccaac ctcggcggct gggatcggct ggtcaaaaaa ggcgggcaca tgctcggcta
    33601 tgctttgctg gcgctcacgc tgctgcgtgg tttcggatct gctggctggc gcaatattct
    33661 gtttgcgttc atcggcgccg cgctctatgc cgccagtgac gagcttcatc agtcttttgt
    33721 cccggggcgc catgctgcct tgttggatgt ggcaatcgac tcgcttggcg ccgcccttgg
    33781 ccttttggct gcccggcatc ttcccgtcct gcagaaaatc attcaccgtg gtctataaaa
    33841 tacccgcctc ttgaaattaa tgacaatcgg tcgcttttgc gctaaactaa attatcgctt
    33901 ttgtcatttt caggagaccg attatgaagc tcaccctgcg cacctatcaa tctgatgacg
    33961 actactggcg cattcgcgat tttttgcgtc aggtaatggt cgctaatcac cgccgtgaat
    34021 ttagctggtc ggttgcccgc tgggattatt ggtggtggtt tgccaatccc gacctggaga
    34081 aaatctccct gaccgaaaat gtgtttatct gggaaactcc ggacgggcaa atcgccgccg
    34141 tcctgaatcc cgaaggccgc gggcaggtct ttttgcagcg ccaccccgat tttatctctc
    34201 ccgaccttga tgccgtaatg attgccctgg ctgaagaccg cctgaccgca ccgaaccagg
    34261 atgaccgccg ccagttgcaa atctttgtcg attcgcaaga caccgccatt caccaaaccc
    34321 tgacccggcg cggcttccaa cgtgttgagc gccccggcga acaagaatat cagcaccgcc
    34381 gctcgctcga tctgcccctg ccgccgccca tcaagctccc cgattacaca atccgtccgt
    34441 tgggtactgg cctggaattt ctagagcgct gctatgcttc aggcctgggc tttcatgatg
    34501 acgatatcca ggtcgcccgt gacaatcgcg atcaccccga ctggtatcgc catatccaat
    34561 cagcgccgct ctaccgccgt gatctggacc tggttgtcat tgctcccgat gggtcagtgg
    34621 cctctttctg caccatctgg tttgacgatg tctctcgcac cgcctacttt gaaccggtcg
    34681 caaccgtacc ggctcaccgt cagcgcggcc tcggcaaagc gatgatgatc gaagggctgc
    34741 actgcctcaa gcagatgggc tgcaaggtag cctttgtggg tggctattct cccggcgcca
    34801 atgccctcta cttttccgtg atgggccccg aatacgatgt ttctgaaccc tgggagaaaa
    34861 ccatctaaac actgccctcg ctttttctct cccggtttgg taacattctg tcgtagcaca
    34921 gatggctaaa atggttagga ggcgctgaat gatcccaatc cgcgattcaa ttcccacccg
    34981 ccgggttcca attgtcaact acgccttgat tggtgcgaac ctgctggtgt ttcttttgat
    35041 gtggttggcc ggaccagaac aagagcggtt ggtctatcaa tttgccctca tcccggccag
    35101 tttttccaac ggcattgatc tgggggatat taccgatatt ttcaccagta tgttcatgca
    35161 cgctggcctt gcccacattg ccggcaacat gctgtactta tggatttttg gtgataatgt
    35221 tgaagatcgt ctgggcagtt tcaaatacct gctcttttat ctggcgggtg gtttggccgc
    35281 ctccctcacc cacctgctct ttaatccaaa ctcccagatc ccaaccgtgg gcgccagcgg
    35341 tgccattgct gccgttctgg gcgcctattt ggtcatgtat ccccaaagcc ggatcgccac
    35401 cttcatcccc ctgggcttct ttatgcggct tgccatggtc cccgcttcgg tggtcctggg
    35461 gttctggttt atcctgcagc tattcaatgg ctttctatcc atcggcgctg ccgatgtcgg
    35521 cggcgtggct ttctgggcgc atattggcgg ttttgtagcc ggggttgtgc tggctaaatt
    35581 aatgcccgcc ccacgccctt ctgccagtgt tatcacctgg taagtttagc ctgtcagacc
    35641 ggatagcgta gctcaggccc catctgcggg ttaacccttc cagatgagaa ttcactcatc
    35701 tcattttgat ttggggtata ctgtctccat tctgctcgat cgattattca ttattcaaac
    35761 tgtgaggaaa acatggtcga aattcaaaaa attgatacaa ccgaccgccg gcaagtgaac
    35821 gaatttgtca attttcacta tcgtctctat gccggtacgc cgcagtgggt cccgccgttc
    35881 tataacgaca tcaagctgat gttgaaccgc gagaaacacc ccttctacga acactcagac
    35941 gccgatttct tcattgcccg ccgtggtgat gaggtggttg ggcgcattgc cgccatggaa
    36001 ctcaaaccct tcaatcaggt tcatggcgtc aaagattctt ccttctacct tttcgacacc
    36061 gtggatgata tcgatgttgc caaagcactg gtcactgctg cggcagaatg ggcccagaag
    36121 cgcggactga ctcgcgtggt cgggcccaag ggcttctccc cgtttgatgg ctacggcatt
    36181 caaattgaag gctttgagca tcatcaaatg atgaccatga tgaactacaa cttcccttac
    36241 tatcaacgcc tgatggaaga actgggtttt gaaaagaaag tggattttgt ttcctgctac
    36301 ctgcaccgtg ataacttcca gcttcccgaa aaagcgcgcg aaatcgcccg ccgggttcag
    36361 gaacgcggta cattcacagt caagaacttc gcctcaaaag ctgagatgct caaatgggga
    36421 gatcgcattg gcgaggctta caataagacc tttgtcaaca actgggaata ttaccccctg
    36481 acccagcgtg aaatcaaatt cacggttgat tctgtgctga ccgtagccgt gcctcaattc
    36541 atcaagatca tcctgcatca cgatgaagtg gttggcttcc tctttgcctt ccccgacatt
    36601 tctcaggcca tccagcgcgc ccgcggccgc ctgacgccct ggagcatcgc cgatatgctg
    36661 atctcgctca agcgcaccaa ctgggtctcg ggcaatggcg ttggcgtcct gcccgaatat
    36721 cagggccgcg gcggcactgc attgatgtat gctgaactgg agaagaccgt tcacacgcct
    36781 caattcgagc acctcgaaat gacccaggtc gctgaatcgg cggttcaaat gcgccgtgat
    36841 ctgattaacc tgggtggcaa ggcttacaag aaccaccgcg ttttccagcg cagcatctaa
    36901 cttaaccata tcccaatccc atcaaaaggc gcacagacgt gcgccttttt tattttcgtt
    36961 tcatgggcag tcggatggct ttgtgcagcg catatcccat taacaacccg atccacggca
    37021 gcaagggaat aacataacgt tgaaacggca gcgttacaaa cccggcctga aaccccaact
    37081 gcagcccaaa gcccaacagc agcaaggcca atcctcgccg ctctgttggt tctttgatta
    37141 cccctcttac tgccagcgca attcccacca acgtcagcgc cagcagaatc cccccgccgc
    37201 caaaactccg cagcagctgg tgcaaggggt ttgccagata atggtcggca tcgctttgct
    37261 gcacttctgc gtaattcccc acatcccaga tggctggtgg tgcaaaatac acctgcgcca
    37321 gtgcacccac caatcgttct ccgggtgtat ccagcgcctg atccgcattt gccgcccgca
    37381 tttccgccgt ttgccgggca gctaactctt gccgagctgt cagcgccgcc tgcgttgcct
    37441 gcaccggata ttgccacagc catggattta acagcccata taccaaccca aatgccagca
    37501 aatacgcccc tgcccggagc agatagcgtg tccagtgaag ttgactgtgt cgaacccata
    37561 gcaccgccac caaccccaac ggcagcagcg tcaccgccga atgtttggcg cagaatgcca
    37621 gtcctgccgc cagccccagc agccaggcag gcgttttctc ccgcagcgcc agccagatga
    37681 acaacgccac acagaataac agcgcacttt cagccatggc ccggcgggtg tgcagcagca
    37741 ccagcgcgtt aatggtcatc aataccagcg ccagacagcc caaccttttg ccgcctaaac
    37801 ttttccctgc ctcataggtg aaccagagcg tcaacgggaa caaccaggcc acacttaatc
    37861 gtcccaccgc cagcagccgc cgatccggca gcgcaccgtt acctgagttt tcttcccaac
    37921 tggctgacca gacccagtca gccggcagtg ctgttgtgtt gcttagcact cggcccaatc
    37981 caattaaagt gcgcgtcagc ggcgcatcca gcagccggta ctgttctcgc agcccgctgg
    38041 tttctgcagg cgtccagaac aaatccgtag ggttttcaaa gagcaattca acgtctgcgc
    38101 tcatataaag ctgtgcgctc tcgtctggat gaaacgggac gcccaaagtc cccgcccagt
    38161 acgggatgca aatcagcaga ataatgccag cagtcagcag tggcagcagt ttctttttat
    38221 tcatacccat aattatatgc ccatgtcgta ttctgacctg acaaattcag caatctcctg
    38281 tacaatcaaa atatgaaaat ctggaaacgc gatatctttt ggctggggct gattttgttg
    38341 gtggcggctg gactcaagct ctggctgctt ctggccgaac gcgtcccctt caacgccgat
    38401 gaggccgtcg ttgcactgat ggctcgccac attttgcagg gtgaacgccc catctttttc
    38461 tatggtcagg cctacatggg cagcctggat gcctggctgg tggcgggtgg ttttgccatc
    38521 tttgggcagc aggtctgggt tatccggctc ctgcaaaccc tgctttatct cggcacaatc
    38581 gccaccacct atctaattgg taaaaccatc ctggggtcat ctcgcaccgg cctgatcgcc
    38641 gccgcactgc tggctgttcc cacggtcaac gtcacgctct acaccaccgc ctccctgggc
    38701 ggctatggcg aggccatcct catcggcaat ctgatgctgc tggcggtaca cgcaatcggg
    38761 cagcgtctgc tgcccgatgc ccctcttgcc ccgcgtgttt ggggtggggt ggtgctctgg
    38821 ggcttcctgg ccgggcttgg cctctggtct aacgccctct cgctgattta tgcaatcccg
    38881 tccgggcttt acctgctctg gaagctccgc tggggctccg gctggcggcg gggttggcca
    38941 attatccccg ctgcgctggt tggctttctg ctgggttcgc tgccctggtg gctctatgct
    39001 gcccaggctg gcctcggaca tctgattggc gaattgaccg gcagtgccgt ggcggttgaa
    39061 aaaggctcct atgcacttca aatactgaat catctcttca acttgattct ccttggcggc
    39121 acagtgacct ttggcttccg ccctccgtgg gaggtgcgct ggctggggct gccgctgctg
    39181 cccttcgtgt tgatgttttg ggctgtggtt atcatcttta tggctcgcgg cattgagcgc
    39241 aagcaggaac attggcccga gcgccgcatg attgtgggcg tgatggtggt cttctggctg
    39301 gcttttattc tgacttcctt cggtgtggat ccttccgggc gctactttgt ccccactgcc
    39361 attcctctgg ccctcattgc cgctgagaca atccagcagg ttagccaaaa gttctggatc
    39421 cgtctcgccc tgattgccct ggtgattggc tttcacatct acggcaccat ccagtgtgcg
    39481 ctgcgttacc cgcctggcat caccacccag ttctatgagc catcggttgt cgatcatcgt
    39541 tacctgccag agttgattga tttcctcaaa accgagggtg aaaccaccgg ctacaccaat
    39601 tattgggtca gttaccccct ggcatttgag tcggacgaaa gtttgatatt tgtaccgcgc
    39661 ctgccatatc acgctgatct gcgctacacc gaacgtgatg atcgctatgc accctacgat
    39721 gccgtagtga tgcaatccac ccgggctgct tacatcacaa cccgcaatgc accgctggat
    39781 gagtacttgc gtgcacagtt taccgctctg ggcatcacct ggcaggagcg tcaaattggt
    39841 gattatcagg tctattaccg gctttcagtc ctggttcgcc cggaacaaat tgggctgggg
    39901 agcagcacgc cgtgaccgtt actgtcaaat catctgctca acaaccgctt gagcgcctga
    39961 ttttggtctt gaccctcgtc ctggtcttcc tcatggctgc ccgcacaccg ttggaaagcg
    40021 atttgtggtg gcacctgcgc gccggcgaag agaccctgca aactggtcag cccatgctga
    40081 ccgacaccct ctccttcacc cgcgccggcg agcattggat caatcactcc tggctctccg
    40141 aggtcgtcat ggcgctggtt taccgcgccg ctggccccct gggactgggc ctgctggttg
    40201 cgctcctggc tgtcctcagc atggggctgg tttaccttca aatggaaggc cctggtttct
    40261 tacgcgcctt tttgatcatc cttgccagcc tggtagctgc cctggtctgg tcgccgcgcc
    40321 cgcagatggc ctctctgctg ggcattgcgg ctctcggcgg catcctgtat ctcaacaagt
    40381 ggcgtcagcg tgacctgctc tggttgatgc tgccgctctt tttgctctgg tctaatctgc
    40441 acggcggcta ttcgattggc ttcctaatcc tggcagccgc acttggcggt gagattctca
    40501 atcacctgct tggcttcacc ggtcccgaag ttctctcctg gcggcggatt gcccgtctgg
    40561 cgttgtggac cggcgcctgt gcgctggtgg tcttaattaa ccccaacggc ctggatacct
    40621 ggcgcatccc cttccagacg gtgggggttc agaccctcca gcaggccata tccgagtggg
    40681 cttcccccga ctttcacgac cttgcccagc agcccttcct ctggctgctg ctggcaaccc
    40741 tcgcctcggt tggactctcg ggtcgccgcc tcgatggtgt ggatctgcta actgtgaccg
    40801 gttttgctta cctcgcgctg gtcgcccgcc gcaattttgg cccctttgcc ctggtcgccg
    40861 ccccggtcct ggcccgccat ctctgggctg ccttgcagtc ctggcccaca cggttgcccg
    40921 cttttcaatc ctggctgaat caattggccg agcgtcgggc ccgcgccaaa cctgctcccc
    40981 cgaccctggc gcgggtactc aatctattga ttgttgccct gctggcgttt gctgctttca
    41041 ttaagttata cattgtgacc cagcccacgt tttacggcgc cctggtgtac agcacttctc
    41101 caaccgccgc ggtgcactgg ctgcaggaaa atcgccccac cggcccgctg ctcagtgagt
    41161 atggctgggg gggctatctc acctggcacc tccccgaata tcctgtcttt gtagatggcc
    41221 gcaccgacct gtttggtgat gagatcatcg gcgactggct gactgtcgtt gaggcccgct
    41281 caggctggcc tgctgtgttg agtcagtggg atgtgcggct catcatgctc gagcccggcc
    41341 gcccggtgat ctctgaatta gatgccgccg gctggacgcg cctctattct gacgatatcg
    41401 cggtgatcta tggacgctga acctcatccc attgaaatta agtcctggcc acgctttctg
    41461 gttggcggtt taatgattgc ccgtctgctc atgctgctgg cgctgccgct ggaagggttg
    41521 cgtggctatg gcgattttca gcatttcttc aatttggctg ccattcccgg ctggccctac
    41581 ctggatcact gggtagaatt cccccccatc tttccctttt tgattgagtt gctatatcgc
    41641 atttccggtg cgcagcagca cacctttgat tacctgcttt tcttcatcct cactgctgcc
    41701 gacgtgggca gtctgctggt ctttggccgc cttctaacac acctcaatcc gccttcggcg
    41761 ctcaagcgct ggctgatgta cgccctggct ctggtagccc tggcctatgc ctggtggtac
    41821 ttcgactccc tgcccgtgct ggctttgctg ctcggcctgc tttggattat cgagggccgc
    41881 gaatggcgcg ccggcgcagt gctgggcctg gggttgatca ccaaactctt cccggcgctt
    41941 gctctggcgg cggcctggcg gctgctgccg ccgcgccgcg ccctgcgggt cacattgctc
    42001 accctgggca taggcctgat tgcctacggc gcgctttatc tggccgcgcc gcagttcacc
    42061 gccgcctcgc tgatttctca gggcgccaaa ggctcctggg agacggtctg ggccctgctg
    42121 gatggcaatc tccgcaccgg gtcgtttggc cccctggtgg aacgccttga gccggctctg
    42181 gcaaatgttt cttcggggca gcctgctcgc attcccacct ggatacccct tggcctcgcg
    42241 gccgctttgg gtttctggcg cttcctgaag gcgcgccgca ccccgcttgc tgctcttgcc
    42301 ctcacgggcc tggcctggtg cctgctcatg ctggcttctc ccggctggag cgtccagtgg
    42361 acgctctacg ctctgccttt catgctcctg ctgctgccag acaaagaagc gcccctgctg
    42421 gcagtcacct tcaccctggt caacctgctg gaatggccca ttttgctctc tcgcggcctg
    42481 ttctgggcca tgccgctcac catcatcctg cgcgccctgc tgctcgtctt gctcgccatc
    42541 cgttttgacc agcgcgcccg tcagccgtaa atatccttcc gccggtcctc aaatactgga
    42601 atcgtgcgtc gtgcctgggc aaccagctca aaatcaaggt cagccgtcag cagcgcttcg
    42661 tccttgccat tgccctcaat cagcgcctcg ccccacggcg tgatgatggc gctgtgccct
    42721 ccatagactg cctcgccaat ttcccctacc gcattcaccc cggcgacaaa catctgattc
    42781 tcaattgccc gcgcccgcag taaggtctgc cagtgcgtta ttcgccgtac cggccactca
    42841 gccactaccc aggcagcctg ggcaccatcc aaagcatacc gccggtacag ctccggaaag
    42901 cgcaaatcgt agcataccga taaccccgct tcgccccagt caaaccgggc ggtttgtccg
    42961 gcttctcccg cgcccagcca ctgctcctct tgcatcaacc ggaagaggtg cagcttgtca
    43021 taggtggctg gcggctgtcc gtcgggtcgc agcagaataa atgaattgaa atatttcccc
    43081 tgccgctccg taagatacga tcctccgatg gcaatctgct cctcccggct gattttcgcc
    43141 agcgtgggca gcagttcagc attttcggcc gcataccggc tggcattcgc caggtcatag
    43201 ccgctggtcc ataactccgg aaacaggatc aatccgctgc ctgcccgggc ggcttcagca
    43261 atcaaccatt tggcctttgc aatattttgt gctggtcttc caatctcaat cttgaactgt
    43321 gccagtgata gtgtgaatat cattgtat
//
LOCUS       DAOPVS010000006        41705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_872_length_41705_cov_56.812989, whole genome shotgun sequence.
ACCESSION   DAOPVS010000006 DAOPVS010000000
VERSION     DAOPVS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 41705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41705
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_41705_cov_56.812989"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PKL11_01400"
     CDS             <1..1202
                     /locus_tag="PKL11_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ12281.1"
                     /translation="IKTCFDPTGLLNPHKILPDLSEASSDLPPVAPLPLQPSYQPQTV
                     DEAAQILAAHTAERQPLWLTGAAAPQITEMRPLSTARLVGIQTLNLDDRYVVAGAGTR
                     LVDLQAELASQGVMIPAASPWPEATLGGVFSAGVNAPLRLCYGALRDIALALDVVLPN
                     GQRIRTGRPVMKNVAGYDLTRLFIGARGSLGLITALTLNLRPLPRRCLTFAVPFEQPA
                     AALAAGLALARSLLTASAVLVGQNLGLPGLPDCTLICTLEGFDEDVAADDAVLHQQLS
                     GRIFVEQPQTGSQIWAAWLAQTLTAHAAVARLGMPISKLPSWVTRSSQHLGPAWLADL
                     GSGLLYAAPTNALACQTAAEAVGGYLAWLGAPGSVAETRRPGIPEWIDRLKSQLDPAG
                     ILPPLVS"
     gene            1323..5606
                     /locus_tag="PKL11_01405"
     CDS             1323..5606
                     /locus_tag="PKL11_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12282.1"
                     /translation="MTSSKSRVRSLSTKLMLAFALVALLPISALAVWNYKAVQSALLS
                     SVDRALLVSASQTATTIDAFLQNNLQAVRSEAAQPILIDFLALPPDQRPESLEEALLR
                     AYLSALESGPSHRLADSPISFVQSFWLLDASGQVVFDTRSLAVGQDSSSEAFFQETLN
                     ASEPYLSPILFSETGAGSLYFAAPVVQSTGSAPLGVLVMRYNADVLQELAARSSNLVG
                     EQSYAILVDENNLILAHGTQPRLRYQRIGALDAVLVTRLQALHRLPPDTLTSQMLDAS
                     QSAAALQTAAENLVLEDALTANIRSQGGRSRLASESWQVIFVQSRQAALNVVRGQLGG
                     AWILAVVVVLLGLGGAWALTRWLTRPITHLQQTAMQVAAGDLSVKARVESADEIGALA
                     QTFNEMTDRLKDSVDGLEQNVAERTVQLDERISQLEVINHVGNYITLLKDDHTLLPTI
                     ADLIRGAFEFYAVLIFVPDRTTAALQLGAASVGDHGQPPSPDLRIPLGCGLIGTAAEQ
                     GEPVNAENAHSDPRCTLIDVLPEVRSELALPLRAGGQMLGVLDLQSSQLSAFSAGDVA
                     VMQTLANQIAVAMYNTRLFTAEAEAHLASHTLREVGQLINSTLDSQEVLERILSALGK
                     VLHYDSGSIMLLEGSDLVIKAVHGFTNPQDVLGVHMPIKKFPLNHQIVTQALPIVLDD
                     VGADNRWQKNRRLPVSGEVHAWMGVPLVSSGKVLGMLAIDNYKPGAYTTNDIPLVTAF
                     ANQAAIALDNARLYAQLHQRAQQLATLNELARIVSGELDRRRILVTLCEQMRQVIPLD
                     SFHGSLYDAETNTLQIAVRYDLGEYRDSTVGPLEQFPWSLKIIQSRQPILYLRTAEEM
                     VAEAEHRRMVGDKTHLSASLMFAPLIVGDDILGVISIQSYNLNAYQPEDLELLVGTAR
                     QTAIALRNAELYEQAQSARAEAEIANQLKTRFMANMSHELRTPLNSIINFAYLLVQGT
                     EGEMNAGQADLLQRIEDSGHHLLGVINDILDLAKIEAGRMELFYEDLDLTDLINGVMA
                     TAVGLVRDRPIELRRQIPAGLPPVHADRTRIRQVLINLISNAAKFTETGFVSISASAD
                     NDFVTVCVEDTGIGMEAEDIPRAFSEFVQLDAGPTRQRGGTGLGLSICKRFVEMHGGR
                     IWAESTPGVGSKFYFSLPRLPSHSFSEPESTEAAETRVLVIDDDRLACDTLAAQLAQG
                     YHVQKLSDSRRAVEAAVQSQPDVIVLDVMMPYMDGWDVLRALRANPVTQQIPVVICSV
                     LNEQQLAFSLQANEYLVKPIERDALRRAVERLAPDGGKVLAVDDDPNALEIVRLSLGS
                     MIYQVAAVQDGQSGLDLAQRDLPDVIILDLMMPGFSGFEVLRALRANPQTASVPVIVI
                     SAKDLTPEEQTQLQAGAALFLQKGQFTADELTRSVRRAVHRKPKGGSHG"
     gene            5599..6024
                     /locus_tag="PKL11_01410"
     CDS             5599..6024
                     /locus_tag="PKL11_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12283.1"
                     /translation="MADVPLSPATVRVLVVEDDANNRLVITRLLKLAGVLSQNIYDTD
                     GDAIALLNTLPPNTVDIVFLDIQLPKKDGYTILQELRADPRYAGLRVVALTANVMRQD
                     IERARAAGFDGFIGKPVDGRRFSETLNRLLTGESVWTVL"
     gene            6039..8462
                     /locus_tag="PKL11_01415"
     CDS             6039..8462
                     /locus_tag="PKL11_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12284.1"
                     /translation="MPDSESARKLRNQALASLAEWVHSTLPLEDILQAAVEGARTILG
                     THQAGLSLTNPDGKSEHQALSTSEKYVDSPDLLQLLLRPELGVLVCQARHPVTVPPRE
                     LPAMRGSLVLPGWMGAPLRRRSGEPLGFLQVADKESGEFDTEDEALLVQITQLVSMAI
                     ENTLLVRAEQNARQVAETLRVANLALTQNLELDSVLETLLDYLGWIIPYDRASVLLVE
                     NADSLTLRSVRNYLAESSEESLQADLVQPRIGALLQQVLADKNTVIAGDLQQDDPMPD
                     WSRIRAWMGVPLVYRTHTIGVVVIEKTDPQAFTEEHQRLADALAAQAVVALRNARLFE
                     ALRESEERYRAATELASDYAYAFRVDENGRFVREWITDAFERITGFEPSEVSTEGGWA
                     SITFPDDRPIALQRIQALLLGQQSVKEFRIVTKDEHIRWVRDHSRPVWDVGRTRIIRI
                     YGATQDITERKQYEREQETIATIASALRLAQTRAEMIPIILHKTLDLLAAEGAALILR
                     DPGGDLEVLAHGQGCWQKAAIPARLAGLSVTDAVIETGQPYWQMDVSKDRRMAPPEDL
                     GGVQAVACVPLMTRQTTLGVLWAGRRTAMIENEVRLLSAIADISANAIHRATLYDELM
                     RAYDATIEALAKSLELRDEPTEGHSQRVADLTLRLARAMGMSEPELIHVRRGAYLHDI
                     GKIGIPDSILQKEDALTEAEWTVMRMHPLYAYKQLSPIAFLRPALDIPHCHHERWDGS
                     GYPRGLKGEEIPLAARIFAVVDVCDALMSSRPYHEPWSLEQTRQFIREQAGAQFDPRV
                     VEAFLQLDL"
     gene            8470..9933
                     /locus_tag="PKL11_01420"
     CDS             8470..9933
                     /locus_tag="PKL11_01420"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNZ12285.1"
                     /translation="MSLASAVHPSRPLIRWPYWLIFALSLAASLVIYIAIFFIPAGLV
                     QWLDRPLLSLYHLDLAYPGLQTQMIAAFAALALLNAAGYWAVRRLQGPAAWALVLVGA
                     LGFALILLYIYPFGAADIFDNIVHGRILGVYGDNPFLNLAAEYPTDPFYNYMWWHKSP
                     SAYGPLWELLAGLTAFLAGNGLVSNVIAFKLLPGFFWLGSTVLLALTLRRHNPDRALP
                     YTLLWAWNPVVLFETFGNGHNDIVMAFFVLAAGCAMLRRQFVPATLALAAGILIKFVP
                     ALLVPPALVAVWRLLPDLRSRLRTFALGGGLSVLLAVACYAPFFTDFDHFYLAHQQTL
                     FTASLPAVLYQMLTTWFHLPGAAAGVAALAWGATGGMVLVQSIRLWHQPSETGFVQAA
                     FAILMFFLLLTCTWFQPWYTLWVFGLGLLLSQESLAAALVLVFATRTKPLLLVPMTYW
                     VRPFPPQPWLEIQLALGILGVPLLFTLVILRFRKNAR"
     gene            10015..11433
                     /locus_tag="PKL11_01425"
     CDS             10015..11433
                     /locus_tag="PKL11_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12286.1"
                     /translation="MKHKFVFLSVVVVLALAMLACSIGDKTPTAVAPTDAPVKTEAPT
                     EAPTEAPVKTEPPTQGGTGELTVVSAYGYQDSWDYYHVTGLIKNDSNKAVSEVELTIE
                     MRDASGATVLKDYDDNFVESTTFNPMLTTLLPGESAPYDYYIYTDMVPETFTVSVSGS
                     LSGDATRAGVEVRNAQLIPGDNGTYAIVGELINNSSTSAKINDLAGVILDGDGNVVAA
                     DWSYDMCNILQPSGDASGMDYAPFAITLDAPTDGSFTDWQVYIDAVETDTEDVAPLYI
                     SFTNNYFDDFGDFHIVGVIENQGTDTYYTSLLGGVYDANGLVVDAGTVSVPLYIKAGE
                     VVPFDVSYWNLVSWNDEIAASIDTFTVQIDPYWTYTVDYDLVDLTNVTTTKDERSSGQ
                     ISLGGTLVNDSGKALYNATVVGYLMDADDNLIAIQWTYVFPEGDAIADGDSNNFEMTF
                     WLDPAVDPNSLTISYMVQGDVK"
     gene            11592..12224
                     /locus_tag="PKL11_01430"
     CDS             11592..12224
                     /locus_tag="PKL11_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNZ12287.1"
                     /translation="MIKVQDVFLPIVLLCMGLLIFVTSLLSPPQMVVEAAPLSPAEQT
                     AQVAQPDPAAAPAAVESTPAEPEPVPAPECAISLAYPESIRQWCGWIDQYAVEYGLES
                     NLIAAVMLQESGGDPNAYSRSGAVGLMQIMPRDGLAASFMCNGQPCFSARPSMTELYD
                     PEFNIAYGVRMLAGLIDRRGSVREALFSYGPIGIGYEYADIVLRIYENYR"
     gene            12339..12515
                     /locus_tag="PKL11_01435"
     CDS             12339..12515
                     /locus_tag="PKL11_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12288.1"
                     /translation="MRKKRLRTILGLVILILSLALLLWASLPLPHETQTIPMPSLEPL
                     LTPTGWEGCLSWLI"
     gene            12503..13402
                     /locus_tag="PKL11_01440"
     CDS             12503..13402
                     /locus_tag="PKL11_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12289.1"
                     /translation="MADLTRRRRFLWSLIILALAVLACNLPAAAPPTTLPTATIQPSI
                     SLPTDLPTAAPLTTQLPTATAPMVIQPTPTRFGIQVATPTLPPVQPTGEALFTPFATL
                     PQPTLATPPSTEIQPALPEQRLLSLEWPSRLRVGDSDIVKLTLEVDASGNITPTAEIG
                     GHEITGQPVFIPNLYDTHDLVLESRLDLAGIQVEPSGSLSSPLQPGTTVTFYWSLSAD
                     HPGTYRGTLWVYLNLIPRDGGPSQQQVLLGKRLDIEAQNLLGLTGGAARTTGVIGAVF
                     GFILNIPFLEDILKFFARRLRRR"
     gene            13506..13862
                     /locus_tag="PKL11_01445"
     CDS             13506..13862
                     /locus_tag="PKL11_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNZ12290.1"
                     /translation="MLRDWLYIGIFLLISLFLPAAAIIMAALLSPRKPNPQKNDTYEC
                     GVETVGESRVQFKVQYYVYGLIFLVFDVEAVFLYPWAVAYNQLALYAVVEAIIFILIL
                     LGGLAYAWRKKALEWQ"
     gene            13890..15116
                     /gene="nuoH"
                     /locus_tag="PKL11_01450"
     CDS             13890..15116
                     /gene="nuoH"
                     /locus_tag="PKL11_01450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNZ12291.1"
                     /translation="MIDFLRDPIAFLTQWLNTTLLGWGLSEQAAQITGFAIGAFVAAT
                     GAMMFVTILIWAERKLIGRIQDRYGPNRVGPFGIFQSIADLVKIFIKEHIDPVGMDWL
                     PFNLAPVLSVAAVLTMWVAIPFAATVYGVDFNVGLLYLVAVGGLGELAVIMAGWGSNN
                     KYALLGAFRAVAMFISYEVPMVITLLIPVMLTGSLSMNDIVRQQNVWFIVVSPLAALI
                     FFISQVAEVGRSPFDLTEAESELVSGFNVEYSGLKFGMFYVADFLHAFTISLLFSALF
                     LGGWRGPGAEEIPILGFIYFAIKSSLVYFLVILMRGSLPRFRVDHMMDFNWKLLTPLS
                     LVLVVLTALALKLMTGLSLWVQIPVLLVLNGLVFLMAQRMVTGFFRRRRPREVGDRSR
                     PVAHYTPPQSDSEAQG"
     gene            15119..15652
                     /locus_tag="PKL11_01455"
     CDS             15119..15652
                     /locus_tag="PKL11_01455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNZ12292.1"
                     /translation="MTPMQITFILIAAVTLVSAVMAVTARRMMHAALWLIATLMGVAM
                     LFATLQASFFAVVQVMVYVGAIAILILFAIMLTRRSTLESDQASLGRGWIINALAAVG
                     VLAALVTAVWAWPAAETVLTELPKDAEQLGLLGQIIADPNGFAVPFEVSSVLLVAVLV
                     GAIYLAVERKPQRGGRQ"
     gene            15649..15969
                     /gene="nuoK"
                     /locus_tag="PKL11_01460"
     CDS             15649..15969
                     /gene="nuoK"
                     /locus_tag="PKL11_01460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNZ12293.1"
                     /translation="MIPMSWYLVIAAALFCVGLFGVLSRRNAVGILIGVELMLNAVNI
                     NLLTFWHYYIQVNAEPPLNALVFTAIVFIVAAAETAIGLALIINAYRQRSTVIANDLN
                     LLKG"
     gene            15975..18134
                     /gene="nuoL"
                     /locus_tag="PKL11_01465"
     CDS             15975..18134
                     /gene="nuoL"
                     /locus_tag="PKL11_01465"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNZ12294.1"
                     /translation="MTGTLLWLLPLFPLVAFVLILLFTYKQHALSHTLALIGAGLAWL
                     GSMVVFYQAVIAPHLAESPIGSSINWLPTGETFFQIGVQVDPLSAVTLFFVAWTILMI
                     FIYSIGYHNFGAKRGVDDQPGLPPHGALVKDKNGHKHHVASVEPMYARFFALISLFAA
                     AMYLLVVSNNLLTLYVGWEIMGLCSYLLIGFWYGKPSARDAALKAFLTTRVGDVFMLL
                     GLAALYTLTGSLNYQAILSNPFILEQLAATTSPVLGLSWAGLIGLLLFIGVVGKSAQF
                     PLHIWLPDAMEGPTPVSAMIHAATMVSAGVYLAARFFPLVSAGWEPGLPLTPTMTIMA
                     AIGAGTALFAATIALAQNDIKRVLAYSTISQLGYMIAALGIGAYAAAVFHLVTHAFFK
                     ALLFLGSGSVIHGMEHGVMHTGEHIDPQDMWQMGGLRKKMPATFWTFLAGGLALSGFP
                     LLTAGFWSKDEILSGAFGGGQIVVFIVLALAALLTAFYTMRQITLTFLGQPRTNAAEH
                     AHESKPVMTIPLMILSLFAIGAGWAGIPEAFPGLGGLIPNWFGGFVGSMVHFEHHAEA
                     HSLVPLFTSLGVSLGGLLLGWLVYRRAGAVDPLEKALGPVHTLLKNKYWVDEIYAVLF
                     IRPARWLADVFVSQWIDRRILDGILHGIGRLGLWLGKLVRQGFDTPVVNGAGDGLANG
                     TRSLGAVLRGLQTGRVQDYMLLAILLAVVAGVLVIVL"
     gene            18159..19730
                     /locus_tag="PKL11_01470"
     CDS             18159..19730
                     /locus_tag="PKL11_01470"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNZ12295.1"
                     /translation="MDWIQNHLLSLILFTPTVAAVIVMLLPAARLKLVRWTALGFSLI
                     PFILTLVLWAQFDPEINAMGAFQFKEQAAWYAAIHATYSLGVDGISLTMVLLTTLLTP
                     LAILASFNITERVKAYMALFLVLETGMLGVFLALDLLLFFVFWEIGLVPMYFLIAQWG
                     GANRNYASLKFMLYTIGGSLGLLLATQVIGLSTGTFDLQLLMVRWPNLTEIGLPLGLS
                     VATVKAIAFWAFVIAFAVKVPIWPFHTWLPDAHTEAPTAGSMILAGVLLKLGAYGFIR
                     LVLPLFPTEATIYGPWLAALATLAIILGALAAWGQKDFKRLVAYSSINHMGFVVLGIA
                     AAAMAPRGLAPDGALSANIALNGAVLQMFNHGLSSAAMFFLVGVIYDRTHTRQLEDFG
                     GLWTVLPVYGTLLVLTSMASLGLPGLNGFVSEFMVVRGSWPLLMLPTALSMIGLFFTG
                     AYILKAIKSVLHGPLNARWVGVLQEITGRELAVITPLVILMVALGLWPMWLLQPINTS
                     VAYLLNNIFSIVNLW"
     gene            19743..21212
                     /locus_tag="PKL11_01475"
     CDS             19743..21212
                     /locus_tag="PKL11_01475"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNZ12296.1"
                     /translation="MTFPFLSLSIFIPLLAGLIILLLPARFKSWVRGIALGAAAITLL
                     LSIIIYARYDTAAGGYQFIEHLPWVPALGISYYVGVDGISAPLVLLAGIVVFCGVLVS
                     WKVEERINEFFAFLLFLGTSVLGVFASLDLFQLFFFFELAVFPKYLMIIIWGSPKTRE
                     YGGMKLTLYLFLGSVIALVGALAIYFGSGLQTFDMLALEKAGFATEFQRLWFPFVFLG
                     FGILGGIFPFHSWAPDGHVAAPTAISMLLAGVEMKVGVFSALRVGIMLLPDGAQMWAP
                     LILILGAINVVYGAFIALIQTDFKYVIGFSSVSHMGLVLIGMATLSPQGLLGAGMQMF
                     SHGVMTALFFAVVGMVYDRAHTREISQLGGLFKKMPWVAMGFIVGGLVSMGMPGFSGF
                     VAEYPIFMGAWRTAPLVAVVAVLGILITAAYILLVVRRVFFGSLPEALAAVSDISWRD
                     KLVIGLLSLTMIALGWFPGLMTPLLVQGVNYILALVGGA"
     gene            21212..22669
                     /locus_tag="PKL11_01480"
     CDS             21212..22669
                     /locus_tag="PKL11_01480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNZ12297.1"
                     /translation="MEIATYTPAMLLAILPEIGLVVLAAVLLILDLVWGDRRCGRLGW
                     FTAGGMVVIMALSLAFSRPTPEAQLIFGGMLRLDMAGFVFRMLFLAGAALTALFSLQD
                     EDLCLKGEFFVLLTVSTLGLSLMASAADLIMLYLAMETASLPLYAMAGFRLHNPRSSE
                     AGIKYLLFGAMSSALMLYGFSLLYGLGGVTNLYALGAALSAGDVPFLTAAIAALLALA
                     GFAFKISAVPFHFWAPDVYHGAPTQVAGFLSTASKAAGFVILLRALAVIFPVVSGFWP
                     VVIIILSLASMTIGNLLALAQKSLKRLLAYSSIAQAGYILVGVAANNEIGAAGAIYYL
                     ISYLVTNLLAFGLVMVVEHHAGTDDLSVFAGLDRRSRALGLLMLTAMLSLGGIPPFSG
                     FFGKLWVFGGAVQANLWWLALIGLLNSVIGLYYYLRVLKVVYLDPASGEFSVSVIRGW
                     AIALGICALGVILLGFIYAPAYNWALQAAQTLLTY"
     gene            22708..23016
                     /locus_tag="PKL11_01485"
     CDS             22708..23016
                     /locus_tag="PKL11_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNZ12298.1"
                     /translation="MSSTSPDPQKKQRAINTTIAILAGQVGCLTLFVLAGAVLGGMWL
                     DARLGSKPTFTVGLLLVSIPVSVILMLVVARKAIAKIKTQPANPQSTRDEEAAVDKKA
                     "
     gene            23000..23995
                     /gene="atpB"
                     /locus_tag="PKL11_01490"
     CDS             23000..23995
                     /gene="atpB"
                     /locus_tag="PKL11_01490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNZ12299.1"
                     /translation="MTKKRKFFSGKHRWWVLLFIVLGIVSSFIPLLKPVQPHLQVAPE
                     KLVEEPLFTLPVLGDFYLFNTLPTLILTLILIVIFAYAIKRSAQKMEKDLVPRGLAGA
                     FEALVETLYNMTENSAGSKFARQIFPWFAAFMIYVLFANLLKIIPGFETIGIMHHAAE
                     GGHDIQGLFGNWYTLLSGEAHGEGYVLTPFLRGISVDLNFTLGLALISVVMTQVIGFR
                     AQGLGYLLKFFNVRTLFSKPFFGAMDFLVGLLELISEFSKIISFAFRLFGNMFAGVVL
                     VALIGTMLPVFLPSFIYLFEFFIGLIQAFVFGMLTMVFMSMVTHSHGGEEHAEAH"
     gene            24032..24271
                     /gene="atpE"
                     /locus_tag="PKL11_01495"
     CDS             24032..24271
                     /gene="atpE"
                     /locus_tag="PKL11_01495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNZ12300.1"
                     /translation="MEEAIITAGRYIGAGLSMIGALGGGIGIGLSVQGALEGMARNPD
                     SYGNLLTNMILGIAFSEAIAIYCLVISFLLLFKIV"
     gene            24293..25030
                     /gene="atpF"
                     /locus_tag="PKL11_01500"
     CDS             24293..25030
                     /gene="atpF"
                     /locus_tag="PKL11_01500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNZ12301.1"
                     /translation="MDKLGINLGFLVVQILSFLTIFVVLKAWAFKPIMNMLEKRRERV
                     AQGLEDARIAAEARANAEREAAAVVAEAQSKAAEVMREASTRAESVQREYRAQAEADI
                     AKAREASMAEVEQERNRMLGELRGQVVTLAMAAAQRVIQDSMDEKRQHALLDEFFSGV
                     KNGKVVVLEGESLTGQAAEVVSALPLNEKEQATVLQEMKSMLGGTPAVNFRVDPAILG
                     GLVVRVGDRVVDGSFAGQLASLRQNLQ"
     gene            25142..26707
                     /locus_tag="PKL11_01505"
     CDS             25142..26707
                     /locus_tag="PKL11_01505"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNZ12302.1"
                     /translation="MPLLSELIRHLPVPPLSRSGSTDPEITGVVLDSRAAVPGSLFVA
                     LVGGSVDGHRYIPSTIQRGAAAAAGTRPASEFADLGVPYFQVADDRLSLAYLAAAFYG
                     FPARQLTVIGVTGTDGKTTTSNLIYQIMQAAGIRTGMITTINAVIGDTALDTGFHVTT
                     PESPDVQRYLAQMVAAGLTHVILETTSHGLAQHRVAACDYDIGVVTNITHEHLDYHGS
                     LEAYRAAKARLFIGLAETAPKPQGNPRLAILNADDDVYDYLRDLTPVRQIAYSLSAGA
                     ADLYAADIAYDASGIHFTAVGPGFQQPIFSPLVGAFNVSNCLAAIAATVLGLGVAPSQ
                     AAAGIAALMGVPGRMERLDMGQNFTAIVDFAHTPNALRRAIEAVRPMTSGRVLAVFGS
                     AGLRDREKRRLMAEVSAELADLTYLTAEDPRTESLDEILAEMVGAACARGAVEGSTVF
                     RIPDRGEAIRRAVQTAQPGDVVICCGKGHEQSMCFGEVEYPWDDRIALRAALAQHLGL
                     PGPQMPRLPTTLS"
     gene            26836..27393
                     /gene="frr"
                     /locus_tag="PKL11_01510"
     CDS             26836..27393
                     /gene="frr"
                     /locus_tag="PKL11_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ12303.1"
                     /translation="MIKETIKDAESRMKGALDNLELDFSGIRTGRASPALVERLPIDY
                     YGAPTPLMQLATISVPEPRALLIRPFDPSTLKTIERAILASDLGINPSNDGKSIRLNL
                     PPLSEERRRDLVKMVHNRMEEARVAIRNIRRDVIKDLKEYEHEKLISEDELKKAEEDL
                     QKLTDQMVQQVESIGERKNKEVMEV"
     gene            27403..28173
                     /locus_tag="PKL11_01515"
     CDS             27403..28173
                     /locus_tag="PKL11_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNZ12304.1"
                     /translation="MTPKKPAEAVPELQKIPRHIAIIMDGNGRWAAARGLPRLAGHRA
                     GTENLRRVLRACVEFGVPYLTIYAFSTENWGRPEEEVQGLMNILGEVIERELNELHQQ
                     GVRLRHIGRLEQLSDKLRASVEHAMEITRNNDKLTLCIAWNYGGRDEIVCAVRRMMAE
                     GIQVDDVNEETISRHLFTAGIPDPDLIIRTSGEMRISNFLIWQAAYAEWYVTPVLWPD
                     FDKEELRKALLSYQQRDRRYGRLSSSAECLPENGSDVA"
     gene            28163..28969
                     /locus_tag="PKL11_01520"
     CDS             28163..28969
                     /locus_tag="PKL11_01520"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ12305.1"
                     /translation="MLRERLIVILILLPIGVALAALGGWFFTIFVAVILGVAAWEYAR
                     LFIGGGYQPSRTLLIAGVALLAVARYLWGESGSIPLLVGLTLLAMGYHTIAYARGQQT
                     SAFDFAITAAGILYVGWLGSYLVSLRSLSGGLYWALLVIGSIAIGDGFAYLVGHLLGR
                     HKMAPNLSPGKTWEGYIGGILGAIAGGALLGWVFGLMVPEISPRLGLLFGLILGVIAP
                     LGDFGESMLKRQFGVKDSGRLLPGHGGILDRLDSWFWAAPIGFYLILWLV"
     gene            29080..30069
                     /gene="glpX"
                     /locus_tag="PKL11_01525"
     CDS             29080..30069
                     /gene="glpX"
                     /locus_tag="PKL11_01525"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNZ12306.1"
                     /translation="MNETKPTRNLALELARVTEAAALAAGRYMGRGDKIAADKAAVDA
                     MRLVLNTVDMDGVIVIGEGEKDEAPMLYNGEHLGLGVPPEVDIAVDPIDGTRPLAFGR
                     HNSISTVALAPRGAMFNPGPFVYMEKIAVGPEAKDVIDIEAPVSVNLRAIARSKGADV
                     NDLTVVILDRPRHESLIAEVRRMGARIRLIPDGDVAGALMAAMPETGIDVLLGTGGTP
                     EGVLAAVALRCMGGTIQGRLAPRSQDELERGLAMGYDVKKILTIDDLVNSEDVFFSAT
                     GITNGDLLRGVDYFGDGASTDSLVMRGLTGTVRRITATHRLGKLSRISEIPYE"
     gene            complement(30127..30861)
                     /locus_tag="PKL11_01530"
     CDS             complement(30127..30861)
                     /locus_tag="PKL11_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNZ12307.1"
                     /translation="MKSKFMLIAVSALAVLATLTACSGLGATNTNNCCGQTQLRTISV
                     SGHSVVYVVPDIAYINIGVHSDGDTVTEALNGNNSQAQAIANTLSEFGIDPKDIQTTA
                     FNVYPNQEYNSEGQVVRKFFSVDNTVYVKVRELDKLGQILDAVVRAGANQINGISFDV
                     ENRTEAEAQARTAAIADARMKAEELAAAAGVTLGELQTASVYNSGGAIPVYEGKGGYM
                     SADASVPMSAGQMQVTADASLTYIIK"
     gene            complement(31015..32097)
                     /locus_tag="PKL11_01535"
     CDS             complement(31015..32097)
                     /locus_tag="PKL11_01535"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNZ12308.1"
                     /translation="MSEDTSVNRHHKVERRGIFWPLALILIGALFLLSNLGVLNANFG
                     SLIATYWPVIFIIGGLDDIYKRENFVGAAVGIGLGTALILGNLGWLPWDAFGLLLRLW
                     PVFLVAWGVDLVVKGRPAWATVLGALLVILVMGGILWLSMTQTPQNAGQTQSISAVLP
                     ANAQEANVSIESPVGQLIVGGPAASGQLIDGKVALLQGLNANENLNSSGSVARYSLSA
                     EGRTTIWPNMNYLSQPGWDLKLTDTLPVTLNTELAVGEQRLNLDGLQIEGLTASIAVG
                     QIVLTLPDDDGFTGTVECAVGEIIIRVPEGANVRIHNHSGLAPVFAPSDYERSQGLIE
                     SPDSDGELIELDVTQAVGLVRIEVIR"
     gene            complement(32110..32736)
                     /locus_tag="PKL11_01540"
     CDS             complement(32110..32736)
                     /locus_tag="PKL11_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12309.1"
                     /translation="MKTNSWRLFAGVGLILAGGLFLLSSLNILDIGGLVIGGFFMLGG
                     LAFLFHLMTNRSAWWAAIPGCVLVGIGAAILLGNLGDGRWSGMAVLGGIALAFWLVYL
                     LNTANWWAIIPAGVMTTLVAVTGLEGLQVDEAGIFLFGLAITFALVALLPSMGTRKTW
                     AWWPAGILAIIGVLALFSAMDWMNYLWGVGLILAGGFLAWRALRPAKS"
     gene            complement(32756..33562)
                     /locus_tag="PKL11_01545"
     CDS             complement(32756..33562)
                     /locus_tag="PKL11_01545"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNZ12310.1"
                     /translation="MRANRLFWGILLALIGVILLLQNVGFLPGNFWTIFWPSLLILVG
                     LWVLLRPRLRQNQAGSLETESLSLPLDSGITEARIKFEHGAGRLQIAAGDNPIELVGG
                     TFVGGVESHVDTTQSIPSVKLSARDNFVAGPVFLGGEGLHWDVRLNRQIPLYIKLSTG
                     AGETRVDLTGLLVKEIKLETGASSTEITLPDRAGQTHVEVEAGAATVKLRVPEGVAAR
                     IKAETGLSSVTVDTARFPRQDDVYISPNYDMAANRVDIDIEGGVGSFDIR"
     gene            33749..34126
                     /gene="acpS"
                     /locus_tag="PKL11_01550"
     CDS             33749..34126
                     /gene="acpS"
                     /locus_tag="PKL11_01550"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNZ12311.1"
                     /translation="MRTGLDLIEIDRLEQLDPSIRRRFLARVFTPAELAQAGDSNPSL
                     AGRFAAKEAVAKALGTGIGVVHWQDIEILTLASGEPQVHLHGAARLLASDLKLTEWSL
                     SITHTRNLAAAFVVALAAPEPGA"
     gene            34123..34812
                     /locus_tag="PKL11_01555"
     CDS             34123..34812
                     /locus_tag="PKL11_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ12312.1"
                     /translation="MKILAVSDVELGFIYNPQIAQRFKDVDLAISCGDLPYYYLEFII
                     SMLDIPLYHVRGNHASKVESGTSGDRAEPWGAIDLHRRAVRDETGLLLAGIEGSLRYN
                     DGPNQYTQGEMWLMCCLLTPQLFLNRVRHGRYLDVLVTHAPPFGIHDLDDRPHQGIKA
                     FNWLNRVFQPTVHLHGHIHVYRQQTVTETQVNQTWVINTFGYREFKLQLPAANQRGRA
                     LYHIEGKRVKS"
     gene            34809..35780
                     /locus_tag="PKL11_01560"
     CDS             34809..35780
                     /locus_tag="PKL11_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12313.1"
                     /translation="MTPTDPPGVFGPGRLAKEDFDAALRKGFWRSVFSWLRRSDNQLL
                     PFDEFRRHLPMMGQSDIGMRQIPIDRIVGSVGRYRDFDGAFLPRTERSRPRWESIDRA
                     HIQQVPLPPIEVYKIGEIYFVKDGNHRVSVARERGQVYIDANVIEIVVPFPVDEKTDI
                     NALILQWELSQFLEKTGLKSLRPNADIRLSMPGLYNRLLDHIQTHGYFMAQDYQREIA
                     WEEAVTSWYDDVYWPLVSVIRQQNILSDFPGRTEADLYLWIIEHLWYLRQEFEDISLE
                     DAALHFTASYAARPGAHPLEKILGGVHSLAVDDGEKTPPTPGGAEGI"
     gene            complement(35809..36039)
                     /locus_tag="PKL11_01565"
     CDS             complement(35809..36039)
                     /locus_tag="PKL11_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12314.1"
                     /translation="MNVGMLWFDNDPKLDLAAKISAATTYYKAKYGQQPNLCFVHPSM
                     LPENGPPPRGVEVRSNRMILPNHLWIGVQEAA"
     gene            complement(36064..36801)
                     /locus_tag="PKL11_01570"
     CDS             complement(36064..36801)
                     /locus_tag="PKL11_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12315.1"
                     /translation="MQLTRTLNSLVVAPEKTPATLTLDDILVDFPYLPVQTAVLGQGE
                     DGYPVLFDLNDPRPGPLLVTAENGCGKTNLLKVVANSLARNNSRYDVQVSILSPKPEE
                     WGGYGGVYPTWERAAGDQVVELAAIAEQRRWGRELGAAQVLLLDGLEQVKRWDDDPQI
                     YFDWMLREGPAMGVWTIATVSPEESLSLGRLMTGFRTRLLGRVEAHSTARRLAQSDEG
                     PQRAMTGVGKFSVQIGGSWLAFQAPLA"
     gene            complement(36918..38804)
                     /locus_tag="PKL11_01575"
     CDS             complement(36918..38804)
                     /locus_tag="PKL11_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNZ12316.1"
                     /translation="MAITPDGRFALSLNYQYRELIIWDFETGQRVDRGNWAGYDKVHW
                     MYLTPDGACAVVVGETGGVVGIPSADPSQVQAWCQRRRPSGEYVSVAANGRRALVNDG
                     EMRVWDFENHCPGVTLPRPAGKAGSNAYYRSAPVISPDGRYGFCRYGCLMVGWDLDRG
                     EIIWQREAINDLYVPLVLGPNPKQILVGMGFKINCWNWETDQLEEPDLPARSSRSTAL
                     AFTPDGSRLISGHYDGSICIWRWPSGILERVLGGHDTGAISQLAVSPDGAFLLSGGGD
                     TSLRKWRIDTGEQIFCTLKTQGTPDPLSIPSQGGIVITPYGCDRATLAVRDLATGEIR
                     TRLEGHQGNVNVLAYLEDGKRLLTGANDQTVRHWDLTTGRCLGVMQGGHAGQVMAIST
                     GAKGTRLVSGAGDGSIIEWDLRSGALIRKLLQYQPPDSRYHKVYLDPRLNWALTGVAK
                     INPQYINYDAFDWYLWNLDASTERSQWYGTGAAHAVVGHWFVQVSGLKYNQVDIVDLL
                     DGKKKQYGFGYRVDTNWAVLPDQEMLYTTGGFFDPKTGQVGGAFETTLGRMTAAAVNQ
                     DLKFAVTCADLKVKLWDLKSRKELAMIEVEKYLFSLAIDPMGGYIIGGNEDMRMSCFR
                     IERH"
     gene            complement(39228..39836)
                     /gene="cysE"
                     /locus_tag="PKL11_01580"
                     /pseudo
     CDS             complement(39228..39836)
                     /gene="cysE"
                     /locus_tag="PKL11_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            complement(39841..40773)
                     /gene="cysK"
                     /locus_tag="PKL11_01585"
     CDS             complement(39841..40773)
                     /gene="cysK"
                     /locus_tag="PKL11_01585"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNZ12317.1"
                     /translation="MKIANDITELIGNTPLVRLRRLTAGSPATVAAKLEYFNPAHSVK
                     DRIGVAMVDAAEQAGLIGPETILLEATSGNTGIALAFVAAARGYRCALVMPETMSRER
                     RMLLRAYGAELILTPGSEGMAGAIRKAQELAASDPRYLILGQFDNPANPEIHRRTTAE
                     EIWRDTDGQVDILVAGVGTGGTITGIGEVLKARKPGLQVVAVEPAASSVLAGGAKGPH
                     PLQGIGAGFVPKILNTGIYDEIIPVQGEDAFKTARAMAQQEGLLVGISSGAATWAAVQ
                     LANRPENAGKLIVVIIPSFGERYLSTALYADLVE"
     gene            complement(41129..41590)
                     /locus_tag="PKL11_01590"
     CDS             complement(41129..41590)
                     /locus_tag="PKL11_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12318.1"
                     /translation="MIKKSRWRTLWLPLLVIPVGSAIMIALTLALYLGIYLLLESIFF
                     SSNPQGFPAGSLRMGCTIALVGLYLILLRTKLPDLLKAIFLTGPLAASIITTGFFLSE
                     LPFLSVTIMLAVVVICGFLLFRFKKPWFYYYAGAIGALLALAYAWPEAWRF"
BASE COUNT         7968 a        12999 c        10999 g         9739 t
ORIGIN      
        1 acatcaaaac ctgctttgac cccaccgggc ttttgaaccc gcataaaatt ctccctgatc
       61 tatcagaggc atcctcagat ctgccgccgg ttgccccgct ccccctgcaa ccatcctacc
      121 agccccagac agtggatgaa gctgcccaaa tcctggctgc ccatactgct gagcggcagc
      181 cactgtggct gacgggtgcc gccgctcccc agattacgga gatgcgccct ctcagtactg
      241 cccggctggt aggcatccag accctcaatc tggatgaccg ttatgttgtg gccggggccg
      301 gcacccggct tgttgacctt caggccgagt tagcctcgca gggtgttatg atcccggcgg
      361 catcaccctg gcctgaggcg acactgggcg gggtcttttc cgccggggtg aatgcgccgc
      421 tgcgcctgtg ctatggcgcc ctgcgggata ttgcccttgc gctggatgtc gtcctgccca
      481 atgggcagcg catccgcaca ggccgcccgg tgatgaaaaa cgtcgccggc tatgacctca
      541 cccggctgtt catcggtgcc cggggctccc tggggttgat cacagcgctc accctcaacc
      601 tgcgccctct gccccgccgc tgtttgacct ttgccgtgcc tttcgaacag cccgcggcgg
      661 cactggcggc tggcctggca ctggcccgga gcctgttaac cgcctcggcg gttctggttg
      721 gtcagaacct gggcctgcct ggtctgccag attgtacgct gatctgcacc ctggaagggt
      781 ttgatgaaga tgtcgctgcc gatgatgccg tcctccatca gcagttgtcc ggccgcatct
      841 ttgtagagca gccacaaacc ggcagccaga tctgggccgc ctggctggcc caaactctga
      901 cggcacatgc tgctgttgcc cggttgggca tgcccatcag caagctgcct tcttgggtca
      961 cccgttccag ccagcacctc ggcccggcct ggctggctga cctgggcagc gggctgctct
     1021 acgccgcccc cacgaatgcc ctggcctgtc aaacggctgc cgaagccgtt ggcggctatc
     1081 tggcctggct gggagcgccg gggtcggtgg cagaaacccg gcggcccggc attccagaat
     1141 ggatagaccg cctcaaatcc caattagacc ctgccggcat cttgccgccg ctggtctcat
     1201 aaacccggct gtgtttttgg gctaagaaag ctgccgcaaa cgatacgcaa actgccaact
     1261 tctagtacaa ttattcaagg gcttttaata gtttggtgtg tttggcgcgc aggagcaggt
     1321 gtatgacttc atcgaagtcc agagttcgca gcctttcaac gaaactgatg ctggcttttg
     1381 ctttagtagc attgctgcca atcagcgcgc tcgctgtgtg gaattataag gcggttcaat
     1441 cggcgctttt atcatcggtg gatcgcgctt tgctggtttc ggcttcacaa accgccacca
     1501 ccatcgacgc ctttttgcaa aacaatctcc aggcggtccg ctctgaagct gctcaaccga
     1561 ttttgattga tttcctggcc ctgccgcctg accagcgtcc agagagcctg gaagaagcgc
     1621 tcctgcgtgc ctatctcagc gcccttgagt ctggccccag ccaccgcctt gctgattccc
     1681 caatctcatt tgtgcaatcc ttctggctgc tcgatgcctc cgggcaggtc gtctttgata
     1741 cccgcagcct ggctgttggt caggacagct caagcgaagc tttttttcaa gagaccctga
     1801 acgcttctga accttacctt tcgcccatcc tgttttctga aaccggcgct ggcagcctgt
     1861 attttgcggc gcctgtggtc cagtccacgg gcagcgcccc gcttggggtg ctggtgatgc
     1921 gttataacgc cgatgtactg caagaactgg cggctcgcag cagcaacctg gtgggggagc
     1981 aatcctatgc catcctggtg gatgaaaaca acctgattct ggctcatggc acacagccac
     2041 ggctgcgcta tcaacggatt ggcgccctgg atgccgtgct tgtcacccgt cttcaagcgc
     2101 tgcaccgcct gccccccgat acattaacct ctcaaatgct ggatgcttcc cagtctgccg
     2161 ctgctttaca aaccgctgct gaaaatctgg tgcttgaaga tgcactgaca gccaatatcc
     2221 gcagccaggg tgggcgttcc cggcttgcct cggaatcctg gcaggtcatc tttgtccagt
     2281 cgcgtcaggc cgcgttgaac gtggtccggg gccagttggg gggcgcctgg atcctggcgg
     2341 ttgtggtcgt tttgttgggg ctgggcggcg cctgggcttt gacccgctgg ttgacccgcc
     2401 cgatcaccca cttgcagcag acggcaatgc aggtcgccgc cggcgatttg agcgtcaaag
     2461 cccgagtcga atcggcagat gaaattggcg ccctggccca gacctttaac gaaatgaccg
     2521 atcgcctgaa agactcggtt gacggcctgg agcaaaatgt cgccgagcgc accgttcaat
     2581 tagacgagcg catcagccag ctggaagtca tcaaccatgt cggaaattac atcaccctgt
     2641 tgaaagatga ccataccctg ctgcccacca ttgcagacct gatccggggt gcttttgaat
     2701 tttacgccgt cttgatcttt gtccccgata gaaccaccgc cgcgcttcaa ttaggcgccg
     2761 cttctgtggg cgatcacggt cagccaccct cacccgacct gcgcatcccc ctgggctgcg
     2821 ggttgatcgg caccgctgcc gaacagggcg aaccggtgaa cgctgaaaac gctcattcgg
     2881 acccacgctg taccctcatc gatgtgctcc cagaggtccg ttctgaattg gccttgcctc
     2941 tgcgggcagg cggccaaatg ctgggtgtgt tggatcttca aagctcacaa ttgtcggctt
     3001 tttctgccgg cgatgtggcc gtgatgcaaa cgcttgccaa ccagatcgcc gttgccatgt
     3061 acaatacccg cctgttcacc gctgaagctg aagctcacct ggcttctcat accctgcggg
     3121 aagtcggaca gttgatcaat tccactctgg acagccagga agtcctggag cgcattttgt
     3181 ctgccctggg caaagtgctg cattatgaca gcggctccat catgctgctg gaaggcagcg
     3241 atctggtgat caaggcggtg catggcttca ccaacccgca agacgtcctg ggcgtccaca
     3301 tgcccattaa aaaattcccg ctcaatcatc aaattgtgac ccaggcgctc cccattgtat
     3361 tggatgatgt tggtgcagat aaccgctggc agaaaaaccg ccgactgccc gtctctggtg
     3421 aagtccatgc ctggatgggt gtcccgctgg tctcttccgg caaagttttg ggcatgctcg
     3481 ccattgataa ttataaacca ggcgcttaca ccaccaacga catccccctg gtaaccgcct
     3541 ttgccaatca ggctgccatt gccctcgata atgcccgcct gtacgcgcag cttcatcagc
     3601 gggcgcagca gttggccacc ctgaacgaac tggcgcgcat cgtctccggc gaactggacc
     3661 gccgccgaat tttagtgacc ctgtgtgaac aaatgcggca ggtcattccc ctggattcat
     3721 ttcacggctc actctacgat gctgagacaa acaccttgca gattgcggtt cgctatgacc
     3781 tgggtgagta tcgtgatagc acagttggcc cgctggaaca gttcccctgg tcactaaaaa
     3841 ttatccagag ccggcagccc atcctttacc tccgcaccgc tgaagagatg gtcgccgaag
     3901 cagagcatcg gcggatggtg ggggataaga cccacctctc agcctccctg atgtttgccc
     3961 ccctgattgt cggtgatgat atcctgggcg ttatttccat ccaatcctac aacctgaacg
     4021 cctaccaacc cgaagatttg gaactgctgg tcggcacagc ccgtcagaca gccatagccc
     4081 tgcgcaatgc tgagctttac gaacaggcgc agtctgcccg ggctgaggcc gaaattgcca
     4141 accagctcaa gacccgcttc atggcgaaca tgtcccatga actgcgcacc ccgctcaatt
     4201 ccatcatcaa ctttgcttat ttgctggttc agggcactga aggcgaaatg aacgccgggc
     4261 aggcagatct cttgcagcgg atcgaagatt caggccacca cctgctgggc gtcatcaacg
     4321 acatcctcga cctggcaaaa attgaggccg gacggatgga attattttat gaagacctgg
     4381 atctgaccga cctgattaac ggcgtcatgg ctaccgccgt tggcctggtg cgtgaccgcc
     4441 ccatcgaact ccgccgccag atcccggccg gtttgccacc ggtccatgct gaccgaacgc
     4501 gcattcgcca ggtactgatc aacctgatct cgaatgccgc caaatttacc gaaaccggct
     4561 ttgtttccat ttctgcctcg gcggataatg attttgtcac cgtctgtgtg gaagatacgg
     4621 gcattggcat ggaggccgaa gacatccccc gcgccttttc cgagtttgtt cagttggatg
     4681 ccgggccaac ccgccagcgc ggcggcaccg ggcttggcct gtccatctgc aagcggtttg
     4741 ttgaaatgca cggtggccgc atttgggctg aaagcacccc tggggtcgga tcaaaattct
     4801 acttctcact gccccgcctt ccatcccatt catttagcga acctgaatcc actgaagcag
     4861 ccgaaacccg ggtgctggtg attgacgatg accgcctggc ttgcgatacc ctggcagccc
     4921 agcttgccca gggctatcat gtccagaagc tcagcgacag ccgccgggct gtggaggcgg
     4981 cggtccagag tcaacccgat gttatcgtgc tggatgtgat gatgccctac atggatggct
     5041 gggacgtact gcgggctctg cgggccaacc cggttaccca gcaaattccg gtcgttatct
     5101 gcagtgtgct gaatgaacag cagttggcct tcagtttgca ggccaatgag tacctggtca
     5161 aacccattga gcgtgacgcc ttgcgccgcg ccgtagaacg cctggcgccc gatggcggca
     5221 aagttctggc ggtggatgat gaccccaatg ctctggaaat tgtccgcctg agcctgggga
     5281 gtatgattta ccaggtcgcc gctgtccagg acggtcagtc cggcctcgac ctggcccagc
     5341 gtgacctgcc cgatgtcatc atcctggatt tgatgatgcc cggtttcagc ggttttgagg
     5401 ttttgcgggc cctccgcgcc aaccctcaaa ccgcctcggt gcccgtcatc gtcatttctg
     5461 ccaaagacct gacccccgaa gaacagacgc agcttcaggc gggggcagcg ttattcttgc
     5521 aaaaaggtca attcacagcc gatgaactga cacgttcagt tcgtcgggct gttcatcgga
     5581 aacccaaagg aggctcccat ggctgatgtt ccgctttccc cggcaacagt gcgtgttttg
     5641 gtcgtggaag atgatgccaa caaccgtctg gtcatcaccc ggctgctaaa attggctggc
     5701 gtgctgtctc aaaacatcta tgataccgat ggcgatgcca ttgcactcct gaacacactg
     5761 ccccccaaca cggtggacat tgtctttctg gatattcaac tgcccaaaaa agatggctac
     5821 accatcctgc aggaactgcg tgctgacccc cgctatgccg ggctgcgcgt ggtggcactc
     5881 accgccaatg tcatgcgcca ggatattgag cgggcgcgag ccgccggctt tgatggtttt
     5941 atcggcaagc cggtggatgg ccgccgcttt agcgaaacac tcaaccgcct tttaactggc
     6001 gaatctgtct ggacggtgct ttaatagagc ttatcttgat gcccgattct gaatccgccc
     6061 gcaaactccg caatcaagcc ctcgccagtc tggcggaatg ggtgcattcc accctgccat
     6121 tggaagatat tctccaggcg gcagtagaag gcgcgcggac catcctgggc acgcatcagg
     6181 ccgggctgtc gttaaccaat cctgacggca aatcggagca tcaggccctg tccaccagcg
     6241 agaagtatgt cgattctccc gacctgctgc aattgctctt gcgccccgaa ctgggggtgc
     6301 tggtctgcca ggctcgccat ccggtcaccg tgcccccgcg tgagcttcct gcaatgcgcg
     6361 gcagccttgt cctgccgggg tggatgggcg ccccgctgcg ccggcgcagc ggcgaaccgc
     6421 tgggcttcct gcaggttgcc gataaagaat ccggcgaatt tgacacagaa gacgaagcgt
     6481 tgctcgtcca aataacccag cttgtttcca tggcaataga aaacactctt ctggtgcgcg
     6541 ccgagcaaaa cgcccgccag gtcgccgaga cgctgcgtgt ggcgaacctg gcccttaccc
     6601 agaacctgga actcgactca gtcctcgaga cgctgctcga ctatcttggt tggatcatcc
     6661 cttatgaccg tgccagtgtc ctgctggtcg aaaacgccga ctccctgact ctgcggtctg
     6721 tgcgtaacta tctggctgaa tcatccgaag aatcgctcca ggccgatctg gttcaacccc
     6781 ggattggcgc tctgctgcag caggtcttgg cggataaaaa cactgtcata gcaggcgact
     6841 tgcagcaaga cgaccccatg cccgactggt cacgtatccg cgcctggatg ggtgtgccgc
     6901 tggtttaccg cacccacacg ataggcgttg tggtcattga aaaaaccgat ccccaggcct
     6961 tcacagagga gcatcagcgc ttggcagatg ccctggcggc tcaggcagtt gtggccctgc
     7021 ggaatgcccg cctctttgaa gcgctgcgtg aatcagaaga gcgttaccgc gctgccactg
     7081 aactggcttc agactacgcc tatgccttcc gggtggatga gaatggtcgt ttcgtgcggg
     7141 aatggattac cgatgctttt gaacgcatca ctggctttga gccctctgag gtttcaacgg
     7201 aaggcggctg ggccagcatc accttccccg atgaccgccc cattgctttg cagcgcattc
     7261 aggccctgct gctgggccag cagagcgtca aagaatttcg aatcgtaacc aaagacgagc
     7321 acatccgctg ggtacgcgat cacagccgcc cggtctggga tgtgggccgc acccgcatca
     7381 tccgaattta tggcgccacg caggatatca ccgagcgtaa gcagtatgag cgggaacagg
     7441 aaaccatcgc caccattgct tcggccttgc gtctggcgca aacccgcgca gagatgatcc
     7501 ccatcatcct gcacaaaacc ctcgacctgc tggcggctga gggcgccgcc ctgatcctgc
     7561 gcgacccagg cggtgatctg gaagtgttgg cgcatggtca ggggtgctgg caaaaagcag
     7621 ctattcccgc ccgtctggcc gggctttcgg ttaccgatgc cgtcatcgaa accggccagc
     7681 catactggca aatggatgtg agcaaagatc gccgcatggc ccctccggag gacctgggcg
     7741 gtgttcaggc ggttgcctgt gtccccctga tgacccgcca gaccaccctg ggcgtgctgt
     7801 gggctggccg ccgcaccgcc atgatcgaaa acgaagtccg cctgctcagc gccattgccg
     7861 atatttcggc caacgccatc caccgtgcca cactgtatga tgaactgatg cgcgcctacg
     7921 atgccacaat tgaagccctg gccaaatctt tggaactgcg cgacgaaccc accgaaggcc
     7981 acagccagcg cgtggctgat ctgacgctgc gcctggcccg tgccatgggc atgagcgaac
     8041 ccgaactgat ccatgtccgc cgcggtgcct acctgcacga tattggcaaa ataggcatcc
     8101 ccgactccat cctccaaaaa gaagacgccc tgaccgaagc cgaatggacg gtcatgcgta
     8161 tgcacccgct ctatgcttac aagcagctct cgccaattgc cttcctgcgc cctgcattgg
     8221 atattcccca ctgtcatcat gagcgttggg atggctctgg ctacccgcgc ggcctcaaag
     8281 gcgaagaaat ccccctggcc gcccgcatct ttgccgtggt agatgtctgt gacgcgctga
     8341 tgtcttcccg cccgtaccac gaaccctggt cacttgagca aacccgccag ttcattcgcg
     8401 aacaggccgg tgcccagttt gacccgcggg tagtagaagc cttcctccaa ttggatttat
     8461 aatcccatca tgtctttggc ctctgccgtt catccctcgc gcccactgat ccgctggcct
     8521 tattggctga tttttgcgct ctcgctggca gcctcgctgg tcatttatat cgccatcttt
     8581 ttcatccctg ccgggctggt gcagtggctg gatcgcccgc tgctcagcct ctatcatctg
     8641 gatctggcct atcccggttt gcaaactcag atgattgccg cctttgccgc cctggcgctg
     8701 ttgaatgctg ccggttactg ggcagtgcgc cggttgcaag gcccggcagc ctgggcgctg
     8761 gtgctggttg gcgccttggg ctttgccctc atcctgctct atatctatcc gtttggcgcg
     8821 gccgacattt ttgacaacat tgtccacggg cgcatcctgg gtgtttatgg cgataacccc
     8881 tttttgaatc tggctgccga atatcccacc gaccccttct ataattacat gtggtggcac
     8941 aagtccccct cggcctatgg cccgctctgg gaactgctgg ctggcctcac agctttcctg
     9001 gctggcaatg gtctggtgag taatgttatc gccttcaaat tgctgccggg cttcttctgg
     9061 ctcggctcca ccgttttact ggcactgacg ttgcggcgcc acaaccctga ccgggccctg
     9121 ccctataccc tgctgtgggc ctggaacccg gtggtcttgt ttgagacctt tggcaatggg
     9181 cataacgata ttgttatggc tttttttgtc ctggcggcgg gttgtgccat gctgcggcgg
     9241 caatttgtgc ctgccactct ggcgcttgcc gccggtattt tgattaaatt cgtcccggcg
     9301 ctgctggtgc cgccggcgct ggtggctgtc tggcgcctgc tgcccgattt gcgttcacgc
     9361 ttgcgtacct ttgcgcttgg gggtggccta tccgttttat tggcagtggc ctgttacgcc
     9421 ccatttttca ccgactttga tcatttctat ctggcgcatc aacaaaccct gttcactgct
     9481 tcgctccctg ccgtgcttta tcaaatgctg accacctggt ttcacctgcc gggcgctgca
     9541 gccggggttg ccgccctggc ctggggggct accggcggca tggtgctggt gcagtccatc
     9601 cgcctctggc atcagccttc cgaaaccggc tttgtccagg cggcttttgc cattttgatg
     9661 ttctttttgc tgttgacctg cacctggttc cagccgtggt acacgctctg ggtctttggc
     9721 ctggggctgc tgctttccca agaatctctg gctgctgccc ttgtgctggt ttttgccacg
     9781 cgcaccaagc ccctgctgct ggtgccaatg acctactggg tgcgtccgtt cccacctcag
     9841 ccctggctgg agattcaact ggcgttgggt attttggggg tgccgctgct ttttacactt
     9901 gtaattttgc gattccgaaa aaatgcccgt tgaaaaatga ttaatttcag gtaaaatagg
     9961 tttgttgtat aaacttcggg gacgccgatt cccccatttt ttgaaggaga agaaatgaag
    10021 cacaagttcg tatttttgag tgtggtggtt gtactggcct tggccatgct ggcatgcagc
    10081 attggcgata aaacccccac tgcggtcgcg cccactgacg cccccgtgaa aaccgaagcc
    10141 ccgaccgagg cccccactga agccccggtc aagaccgaac ccccgaccca gggcggtacc
    10201 ggtgaactta ccgtcgtcag cgcctatggc taccaggatt cctgggatta ctatcatgtc
    10261 accggcctga tcaagaacga cagcaataaa gctgtctccg aagttgagct caccatcgaa
    10321 atgcgtgatg ccagcggcgc cactgtgttg aaggattacg atgataactt cgttgaatcc
    10381 accaccttca accccatgct gaccactctg ctgcctggtg agtctgcgcc ttatgattac
    10441 tacatctaca ccgacatggt ccccgaaacc ttcacggtct cggtctcggg ctccctgagt
    10501 ggcgatgcca cccgcgcggg tgtggaagtc cgcaatgctc agctgatccc tggcgacaac
    10561 ggcacctacg ctatcgtggg cgaactgatc aacaacagct caacctccgc caagatcaat
    10621 gatctggctg gtgtgatcct ggatggcgat ggcaatgtcg tggccgccga ctggtcctac
    10681 gatatgtgca acatcctcca gccctctggc gatgcctctg gcatggacta tgctcccttc
    10741 gccatcaccc tcgatgcccc caccgatggt tccttcaccg actggcaggt ctatattgac
    10801 gctgtcgaaa ccgacaccga agacgttgcc ccgctctaca tttccttcac caacaactac
    10861 ttcgatgatt tcggcgattt ccacatcgtc ggcgtgattg aaaaccaggg caccgacacc
    10921 tactatacct ccctgctggg tggtgtctat gatgccaatg gcctcgttgt ggatgccggt
    10981 accgtcagcg ttccgctcta catcaaagcc ggcgaagttg tgcccttcga tgtcagctac
    11041 tggaacctgg tcagttggaa cgatgaaatt gccgccagca ttgatacctt caccgttcag
    11101 attgatccct actggaccta caccgtggat tacgacctgg ttgacctgac caacgtcacc
    11161 accaccaaag acgaacgctc cagcggccag atcagcctgg gcggcacact ggtcaatgac
    11221 agcggcaaag ccctgtacaa cgccaccgtt gtgggctacc tcatggatgc cgatgacaac
    11281 ctgatcgcca tccagtggac ctacgtcttc cccgaaggcg atgcgattgc cgatggtgac
    11341 agcaacaact ttgagatgac cttctggctg gatcccgctg tggatcccaa ttctctgacc
    11401 atcagctaca tggtccaggg cgacgtcaag taatctgtcc ctctggtacg ccagtaagac
    11461 aaccgatccc ccgccttttt gacaggtggg ggatcgcttt ttatgcaatc gccttgaaat
    11521 tatagaacat acgtgctatc atcgggtcac cttaaccgaa ccgatacctt tacaacaatg
    11581 gatgtgaccc aatgatcaaa gttcaagatg tatttctccc catcgtattg ctctgcatgg
    11641 gattgctgat tttcgtcacc agcctgcttt ccccccctca aatggttgtg gaagcggcgc
    11701 cgctttcacc tgccgagcag actgcccagg tggcccagcc cgatccagcc gccgctcctg
    11761 ccgctgttga gtcaaccccg gctgaacccg aacccgttcc cgcgcctgag tgcgccatca
    11821 gcctggctta tcccgaatcc atccgccagt ggtgtggctg gattgatcag tatgcggtgg
    11881 aatatgggct ggaatccaac ctcattgccg cagtcatgct tcaggaaagc ggcggcgatc
    11941 ccaatgccta ttctcgcagc ggggcagtgg gcctcatgca aatcatgccg cgtgatggtc
    12001 tggcagccag ctttatgtgc aacggccaac cgtgtttctc tgcccgcccc agcatgaccg
    12061 aactctatga cccagaattc aatattgctt atggcgtccg catgttggcc ggcctgattg
    12121 accgccgcgg cagcgtgcgc gaagccctct tctcctacgg ccccatcggc atcggctatg
    12181 aatacgccga tatcgttctg cgcatctacg agaactaccg ctgattttgt gctgcttttc
    12241 gccccaatcc cctgcccggc cccggcaggg gattttttta tttcttcatc tgcttgccaa
    12301 gcccatccaa aggctgtata gttatatcgg aggtggaaat gagaaagaaa cggttgcgca
    12361 ccatcctcgg cctggtgatc ttgatcctct cgctggcgct cttgctttgg gccagcctgc
    12421 ccctgccgca tgaaacccag accatcccca tgccttccct cgaaccctta ttgaccccca
    12481 ctggctggga gggctgcctg tcatggctga tctaacccgc cgccgtcgct ttttgtggag
    12541 cctgattatc ctggctttgg ctgtgctggc ctgcaacctg cctgccgccg cgccgccaac
    12601 aaccctgcca actgccacca ttcaaccgtc catttcactg cccaccgact taccaaccgc
    12661 cgcgccgcta accacccagc tgcccaccgc cacggcacca atggtaattc aaccaacccc
    12721 cacccgtttt ggcatccagg tggctactcc caccctgccg cccgttcagc ccaccggtga
    12781 agcgcttttt accccgtttg ccaccctgcc ccagcccacc ctcgcaaccc cgccgtctac
    12841 tgaaattcag cctgcgctgc ccgagcagcg cttgctctcc ctggagtggc ccagccgcct
    12901 gcgggtgggt gattcagata tcgtcaaatt aactctggag gtggatgcca gcggtaacat
    12961 cacccccacc gctgaaattg gcggtcacga aatcactggc cagccggtat ttatccccaa
    13021 cctctacgat acccacgacc tggttctgga atcccggctt gatcttgctg gcatccaggt
    13081 tgagccctcc ggcagcctca gctcgccttt gcagccgggc acaaccgtca ccttctactg
    13141 gagcctgtcc gccgatcacc ccggcacgta ccgcggcacc ctgtgggtct atctcaacct
    13201 gatcccccgg gatggcggcc cgagccagca gcaggttttg ctgggcaagc ggttggatat
    13261 tgaggctcaa aatctccttg gacttacggg gggcgcagcc cgcaccaccg gtgtgattgg
    13321 cgcagtcttc gggtttattc tcaacattcc tttccttgaa gatatcctca agttttttgc
    13381 ccgccgcctg cgcagaagat aaaatcccaa ttggctctcc ggtatcaaat attaaattat
    13441 gatacaatat atctgcttca cccttccggt gcggctttcc attctggtgc aatgaaggag
    13501 cggttatgct gagggattgg ctgtacattg gcatttttct gctgatctcg ctcttcctgc
    13561 ctgcggctgc cattatcatg gcggcgctgc tttcaccccg caaacccaac ccgcagaaaa
    13621 acgacaccta tgaatgcggt gtcgaaacgg tgggtgaatc ccgggtccaa ttcaaggtgc
    13681 agtactacgt ctatggcctg attttcctgg tctttgacgt ggaagctgtt ttcctgtacc
    13741 cctgggcggt ggcatacaat cagttggcgc tctatgccgt ggtagaagcg atcattttca
    13801 tcctgattct gctgggcggg ctggcctatg cctggcggaa aaaagccctg gaatggcagt
    13861 agcccaggca gaaaggagat ttgcacccca tgatagactt tctgcgtgac ccgattgcct
    13921 ttctgaccca gtggctgaat accacgcttc tgggttgggg cctatccgaa caggcggctc
    13981 agatcaccgg ctttgccatt ggcgcgtttg tcgccgctac tggcgctatg atgtttgtga
    14041 ccatcctcat ctgggccgag cgcaagctga ttggccgtat tcaagaccgc tacggtccca
    14101 atcgcgtcgg cccgtttggc atcttccaat cgattgccga tctggtcaaa atctttatta
    14161 aagagcacat cgaccccgta ggtatggact ggctgccctt caatctggcg ccggttttat
    14221 ctgtggccgc tgtactgacc atgtgggtcg ccatcccctt tgccgccact gtttacggcg
    14281 ttgatttcaa cgtcggcctc ctttacctgg tggcggtggg cggtctgggc gaactggcag
    14341 tcattatggc aggctggggc tccaacaaca aatacgcctt gctgggtgcc ttccgcgcgg
    14401 tggcaatgtt catctcttac gaagtgccca tggtcatcac cctgctcatc cccgtgatgc
    14461 tgaccggttc attgagcatg aacgatattg tccgccagca gaatgtctgg ttcatcgtgg
    14521 tttctccgct tgccgcgttg atcttcttca tctcacaggt ggctgaggtc gggcgctctc
    14581 cctttgatct gaccgaggct gaatcagaac tggtctccgg cttcaatgtg gaatactccg
    14641 gcctgaagtt cggcatgttc tatgtggccg acttcctcca tgccttcacc atctcgctgc
    14701 tgttttcagc cctcttcctg ggtggctggc gcggccccgg cgctgaagaa atccccatcc
    14761 tcggcttcat ttacttcgcc atcaagtcca gcctggtcta cttcctcgtc atcctgatgc
    14821 gtggatcgct gccccgcttc cgtgtagatc acatgatgga cttcaattgg aaactgctca
    14881 ccccgctttc gctggtgttg gtcgtcctga ccgccctggc gctcaaactg atgactggtt
    14941 tatcgctctg ggttcagatc cccgttctgc ttgttctgaa tggtctggtc tttttgatgg
    15001 ctcagcgcat ggtcacgggc tttttccgcc gccgccgccc gcgcgaggtc ggggaccgct
    15061 cccgcccggt agcccactac acaccccctc aatctgattc tgaggcgcag gggtaactat
    15121 gacaccaatg caaatcactt tcattcttat tgccgcagtc acactggtct ccgcggtgat
    15181 ggctgtcacc gcccgccgaa tgatgcatgc cgccctctgg ctgattgcca ctttgatggg
    15241 ggttgccatg ctttttgcca ccctgcaggc cagcttcttt gctgtggtgc aggtgatggt
    15301 ctatgtgggt gccattgcca ttctaatcct cttcgccatt atgttgaccc gccgttccac
    15361 cctggaaagc gatcaggctt ctctggggcg cggttggatc atcaatgctc tggctgcggt
    15421 gggcgttttg gccgcgctgg tcacagcagt ctgggcctgg cccgcggctg aaacggtttt
    15481 gaccgagctg cccaaagacg ccgaacagct tggcctgctg gggcaaatta ttgctgatcc
    15541 aaatggtttc gccgtcccct ttgaagtctc ctccgtgctg ctggttgccg tgctggtagg
    15601 agccatttac ctggcggtgg agcgcaaacc gcagcgagga ggtcgccaat gatcccaatg
    15661 tcctggtatc tcgtcattgc tgccgccctg ttttgcgtgg ggcttttcgg cgtcttgtcc
    15721 cgccgcaacg ccgtgggcat tctcattggc gtcgaactga tgctgaacgc agtcaatatc
    15781 aacctgctga ccttctggca ctactacatc caggtaaacg ccgaaccgcc gttgaacgcc
    15841 ctggtcttta cggcaattgt cttcatcgtc gctgcggctg aaaccgccat tggtctggcg
    15901 ttgattatca acgcctaccg ccagcgcagc accgtgattg ccaatgatct caatctgttg
    15961 aagggctaaa gactatgacc ggaacacttc tctggctgct gcccctcttt ccactggttg
    16021 cctttgtcct gattttgctc ttcacctaca aacaacatgc cctcagccac accctggccc
    16081 tcattggcgc cgggctggcc tggctgggaa gcatggttgt tttttatcag gctgtcatcg
    16141 ccccgcacct cgctgaatct cccatcgggt ccagcattaa ctggctgccc actggcgaaa
    16201 ccttcttcca gattggcgtc caggtggatc cgctcagcgc ggtcacgctc ttctttgtcg
    16261 cctggacgat cttgatgatc ttcatttaca gcattggcta ccataatttc ggcgccaagc
    16321 gcggtgtgga tgatcaaccc ggcctgccgc cgcacggcgc cctggtcaaa gacaagaacg
    16381 gtcacaagca ccatgtcgcc tcggttgagc cgatgtatgc acggtttttt gccctcatca
    16441 gcctgtttgc cgcggccatg tatctgctgg tggtcagcaa caacctgctc accctctacg
    16501 tgggctggga aatcatgggc ctgtgctcct acctcttgat tggtttctgg tatggcaagc
    16561 cctcggcgcg ggatgccgcc ctcaaggcct tcctcaccac ccgcgtgggt gatgtcttca
    16621 tgctgctggg cctggccgcg ctctataccc tcaccggctc attaaattat caggccattc
    16681 tctccaatcc gttcattctg gagcagttgg ccgccaccac ttcacctgtc ttgggccttt
    16741 cctgggccgg tttgattggc ctgctgctgt ttatcggcgt ggtgggcaag tctgcccagt
    16801 tcccgctgca catctggctc cccgatgcca tggaaggccc cacccccgtc tcggcaatga
    16861 tccatgccgc cacaatggtc tcggcgggtg tttatctggc ggcccgcttc ttcccgttgg
    16921 tttcagccgg ctgggaaccc ggcctgcccc tgacccccac catgaccatc atggctgcca
    16981 ttggcgctgg cacagcgctc ttcgcggcca ccattgccct ggcccagaat gacatcaagc
    17041 gtgtcctggc ctattccacc atctcgcagc tcggttacat gattgccgct cttggcatcg
    17101 gtgcttatgc tgcggctgtc tttcacctgg tgacccacgc cttcttcaag gccctgctct
    17161 tcctgggctc gggctcggtc attcacggca tggaacacgg cgtgatgcac actggcgagc
    17221 atattgaccc acaggatatg tggcagatgg gcggcctgcg caagaagatg cctgccactt
    17281 tctggacctt cctggctggt ggcctggcgc tctcgggctt tccgctgctg accgccggct
    17341 tctggtcaaa agatgaaatc ctctctggcg catttggcgg cgggcagata gttgttttca
    17401 ttgtgctggc tctcgcggct ttgctgacgg ccttctacac catgcggcag atcacgctga
    17461 ccttcctggg ccagcctcgc accaacgccg ccgaacatgc ccacgaatca aagcccgtga
    17521 tgaccatccc gctgatgatt ttgagcctct tcgcgattgg cgccggttgg gcaggcatcc
    17581 ctgaggcctt ccccggtctc ggcggtctga tccccaactg gtttggcggt tttgtgggca
    17641 gcatggtgca ctttgagcat cacgctgaag cccacagcct ggtgccgctc ttcacctcgc
    17701 ttggtgtgtc gctgggcggc ttactgctcg gctggctggt ttatcgccgc gccggcgccg
    17761 ttgacccgct cgaaaaggcg ctgggcccgg tgcacaccct gctcaagaac aaatactggg
    17821 tggatgagat ctacgctgtc ctgttcattc gcccggctcg ctggctggcc gatgtcttcg
    17881 tctcgcagtg gattgaccgc cgcatcctgg acggcatcct gcatggcatt ggtcgcctgg
    17941 gtctctggct gggcaagctg gttcgccagg gctttgatac cccggtggtc aacggcgccg
    18001 gcgatggcct ggcaaacggc acccgctccc tgggcgccgt gcttcggggc cttcaaaccg
    18061 gtcgtgtgca ggactatatg ctgctggcta ttttgctggc tgtcgtggcg ggcgtgctcg
    18121 tcatcgtctt atagagaaag acaggcagag aggttcgcat ggattggatt caaaatcatc
    18181 tgctcagttt gatcttattc acccccacag tcgccgcggt gattgtcatg ctgcttcccg
    18241 ccgcgcgcct caagctggtg cgctggacgg ccctcggctt tagcctgatt cccttcatcc
    18301 tcaccctggt cttgtgggcg cagttcgacc ctgaaatcaa cgccatgggc gcctttcagt
    18361 tcaaagaaca ggctgcctgg tatgccgcca ttcatgccac ctactccctc ggcgttgatg
    18421 gcatttcgct gaccatggtg ctgctcacca cgctgcttac cccgctggcg atcctggcct
    18481 ctttcaacat caccgagcgc gtcaaagcct atatggcgct cttcctggtg ctggagacgg
    18541 gcatgctggg cgtcttcctc gccctggatt tgctgctctt cttcgttttc tgggaaattg
    18601 gcctcgtgcc catgtacttt ctgattgccc agtggggcgg cgccaaccgc aactatgcct
    18661 cactcaagtt catgctctac accatcggcg gttcgctggg cttgctcctc gccacccagg
    18721 tcatcggcct cagcactggc accttcgacc tgcagctcct gatggtccgc tggcccaacc
    18781 tgacggaaat cggcctgccg ctgggcctgt cagtagccac ggtcaaggcc attgccttct
    18841 gggcatttgt gattgctttt gccgtcaaag tgccgatctg gccgttccac acctggctgc
    18901 ccgatgcgca caccgaagcg cccaccgctg gctcgatgat cctggcgggt gtgctgctca
    18961 agctgggcgc ttacggcttc attcgcctgg tgctgcccct cttccccacc gaggctacca
    19021 tctacggccc ctggctggct gctctggcca ccctggcgat tatcctgggt gcgttggcag
    19081 cctggggaca gaaagatttc aagcgcctgg tagcctattc ttcaatcaat cacatgggct
    19141 ttgtggttct tggcattgcc gctgccgcca tggcccctcg cggcctggcg cccgatggcg
    19201 ccctgagcgc caacatcgcc ctcaacggcg cggtgctgca aatgttcaat cacggcctca
    19261 gctctgctgc catgttcttc ctggttggtg tgatttatga ccgtacccac acccgtcagt
    19321 tggaagactt tggcggcctc tggacggtcc tgccggtcta tggcaccctg ctggtgctga
    19381 cctcaatggc ttcactgggc ctgcccggcc tgaacggctt tgtctccgag ttcatggtcg
    19441 tccgcggctc ctggcccctg ctgatgctgc caactgccct cagcatgatt ggcctgttct
    19501 tcaccggcgc ctacatcctc aaggcaatca aatctgtcct gcatggcccg cttaatgcgc
    19561 gctgggtggg tgtcctgcaa gagatcaccg ggcgtgagct ggctgtgatc accccactgg
    19621 taattttgat ggtggccctg gggctgtggc ccatgtggct gctccaaccc atcaatacat
    19681 cagtggccta tctgctcaac aatattttct ccattgtgaa tctttggtag cgaggtgaag
    19741 gcatgacatt tcctttcctg agtttgtcca tcttcatccc gctgctcgcc ggcctgatta
    19801 tcctgctgct cccggctcgc ttcaagagct gggtgcgcgg tatcgctctc ggcgccgctg
    19861 ccattacgct tttactctcc atcatcatct atgcccgtta tgataccgcc gcgggcggct
    19921 atcagtttat cgagcatcta ccctgggtcc cggcgctggg catctcttac tacgtcggcg
    19981 tggatggcat cagcgctccg ctggtattgc tggcgggcat cgttgttttc tgcggcgtac
    20041 tggtctcctg gaaggttgag gaacgcatca acgagttctt tgccttcctg ctcttcctcg
    20101 gcaccagtgt tctgggtgtc tttgcctcgc tggacctctt tcaactgttc ttcttctttg
    20161 aactggccgt cttccccaaa tatctgatga tcatcatctg gggttcaccc aaaacccgcg
    20221 aatacggcgg catgaagctg accctctacc ttttcctggg gtctgtcatc gccctggtgg
    20281 gggcgctggc catttacttc ggctctggcc tgcaaacctt tgacatgctg gcgctggaga
    20341 aagctggttt tgctaccgag ttccagcggc tctggttccc gtttgtcttc ctgggctttg
    20401 gcattctggg cggcatcttc cccttccatt cctgggcgcc cgatggtcac gtggccgccc
    20461 ccaccgccat ctccatgctg ctggccggcg tggaaatgaa agtgggcgtc ttctcggcgc
    20521 tgcgggttgg catcatgctc ctgcccgatg gcgctcagat gtgggctccg ttgatcctga
    20581 tcctgggggc catcaatgtg gtttatggcg ccttcattgc cctcatccag acggatttca
    20641 agtatgtcat cggtttttcc tccgtttcac acatggggct ggtcttgatt ggcatggcta
    20701 ccctttcgcc gcagggcttg ctgggcgctg gtatgcaaat gttctcccac ggcgtaatga
    20761 cggcgctctt ctttgcggtt gtcggcatgg tttatgaccg ggcccatacc cgggagatca
    20821 gccagcttgg gggtttgttc aaaaagatgc cctgggttgc catgggcttt atcgtgggcg
    20881 gtttggtttc aatgggcatg ccgggcttct cgggttttgt cgccgaatat cccatcttca
    20941 tgggcgcctg gcgaaccgcc ccgctggtgg cggttgtagc agtcctgggt atcctcatca
    21001 ccgcagccta tatcctgctg gttgtgcggc gggtcttctt tggctccctg cccgaagccc
    21061 tggctgccgt cagcgatatc tcctggcggg ataagctggt cattggcctg ctctcgttga
    21121 cgatgattgc cctgggctgg ttccccggtc tgatgacgcc gctgctggtg cagggcgtga
    21181 actacatcct cgctctggta ggaggtgcat agtggaaatc gcaacatata cccctgccat
    21241 gctgctggca atcttacctg aaatcggcct ggtcgttctg gcggcggttc tgctgatcct
    21301 ggacctggtc tggggagatc ggcgctgtgg gcgcctgggc tggttcaccg cggggggcat
    21361 ggttgtcatt atggccctca gcctggcatt ttcccgcccc acccccgaag ctcaactgat
    21421 ctttggcggc atgctccgcc tggatatggc cggctttgtc ttccgcatgt tgttcctcgc
    21481 cggtgctgcg ctgacagccc ttttctcact tcaggatgaa gacctgtgcc tgaagggcga
    21541 gttctttgtc ttgctgaccg tcagcaccct gggcctcagc ctgatggcct ccgccgccga
    21601 cctcatcatg ctctacctcg ccatggaaac cgcctcactg ccgctctatg ccatggcggg
    21661 cttccgattg cacaatccgc gctcatccga ggctggtatc aagtacctgc tctttggcgc
    21721 catgtcttcg gccctgatgc tttacggctt cagtctgctc tacggcctgg gcggcgtgac
    21781 caacctctac gccctgggtg cagccctgtc tgccggagat gtccccttcc tgactgccgc
    21841 gattgctgcg cttttggccc tggctggctt tgccttcaaa atcagtgcgg tgccgttcca
    21901 tttctgggcg cccgatgtct atcacggcgc tccgacccag gtagcgggct ttctttccac
    21961 ggcttccaag gccgccggct ttgtgatttt actgcgcgcc ctggcagtca ttttccccgt
    22021 tgttagcggc ttctggccag tagtgatcat catcctgtcc cttgcctcca tgaccattgg
    22081 caacctgctg gcgctggcgc aaaagagcct caagcgcctg ctggcctatt cttccatcgc
    22141 tcaggcggga tatatcctgg tgggcgtggc tgccaacaat gaaattggcg ctgctggagc
    22201 tatctattac ctgatctcct atctcgtcac caacctcctc gcctttggtc tggtgatggt
    22261 cgttgaacat cacgccggta cggacgacct gtctgtcttt gccggcctcg accggcgctc
    22321 gcgcgccctg ggcctcttga tgctgactgc catgctgtcg ctgggcggca ttcccccgtt
    22381 ctcggggttc tttggcaagc tgtgggtgtt cggcggcgca gtccaggcca acctctggtg
    22441 gctggctctg attggcctgc tcaactctgt cattggcctc tactactacc tgcgcgttct
    22501 caaagtcgtg tatctggacc ccgcctctgg cgagttttct gtctctgtca ttcggggctg
    22561 ggccattgcg ctgggaatct gcgctctggg cgtcattctg ctgggcttca tctatgcccc
    22621 ggcgtacaac tgggctttgc aagccgccca gactctcctc acatattgat tgacgctact
    22681 ttttcgcctg tgatataatt ttctcacatg agctcaacca gcccagatcc tcagaaaaaa
    22741 caacgtgcaa tcaacacgac cattgccatt ctggcaggtc aggtgggatg tcttacgttg
    22801 tttgttctgg ccggggccgt gctgggtggc atgtggttgg atgcccgcct gggctccaaa
    22861 cccaccttca cggtgggact gctgctggtc agcattccgg tctcggtcat tctcatgctg
    22921 gttgtggccc gcaaggcgat tgccaaaatc aaaacccaac ctgcaaatcc ccaatccact
    22981 cgtgatgagg aggctgccgt tgacaaaaaa gcgtaaattc ttttcaggga agcatcgctg
    23041 gtgggtgctg ctgttcattg tcctgggcat cgtgtcgtct tttatcccac tgctcaagcc
    23101 agtccagccg cacctgcagg tggcgcctga gaagctggtg gaagaacccc tcttcaccct
    23161 gccggtgctg ggcgacttct acctcttcaa taccctgccc accctgatcc tgaccctgat
    23221 cctgattgtc atctttgcct acgccatcaa acgctcggct caaaagatgg aaaaggatct
    23281 ggtgccgcgg ggtctggcgg gcgcgtttga agctctggtt gaaaccctct acaacatgac
    23341 cgaaaactct gccgggtcca aatttgccag gcagattttc ccctggtttg ctgcatttat
    23401 gatttacgtg ctgtttgcca acctgctcaa aatcatcccc ggttttgaaa ccatcggcat
    23461 catgcaccac gcagccgagg gtggtcacga cattcagggt ctgtttggca actggtatac
    23521 cctgctgtct ggcgaggccc atggcgaagg ctatgtgctc acccccttcc tgcgcggcat
    23581 ctcggttgac ctgaacttca ccctcggtct ggccctcatc tcggttgtca tgactcaggt
    23641 gatcggcttc cgggctcagg gtttgggcta cttgctcaaa ttcttcaacg tccgcaccct
    23701 gttctccaag cccttcttcg gggccatgga ctttctggtt ggcctcctcg aactgatctc
    23761 tgaattttcc aagatcatct cgtttgcctt ccggttgttc ggcaacatgt ttgcgggcgt
    23821 tgtgctggtg gcattgatcg gaaccatgct gccagtcttc ctcccttcgt tcatttacct
    23881 gtttgagttc ttcatcggac tcatccaggc gtttgtgttc ggcatgttga caatggtctt
    23941 catgtccatg gtcacgcaca gtcacggcgg cgaagaacac gccgaagcgc attaatctct
    24001 cggatacaaa accaccacag gagtaactca aatggaagaa gcgattatta cggcaggacg
    24061 ttacattggc gcagggcttt cgatgattgg cgcccttggc ggcggtatcg gtatcggcct
    24121 cagcgtgcag ggcgcgctgg aaggcatggc ccgcaacccc gactcatacg gcaatctgct
    24181 caccaacatg atcctgggca ttgccttctc agaagcaatc gccatctact gtctggtgat
    24241 ttccttcctg ctgctcttca agatcgtcta accctcgcca aaggagtgca ccttggataa
    24301 actgggtatc aatcttggct ttctggtggt ccagatcctg tccttcctca ccatttttgt
    24361 ggtgttgaag gcctgggcct tcaaaccgat tatgaatatg ctggaaaaac gccgcgagcg
    24421 tgtggcgcag gggctggaag atgcccgcat cgccgccgaa gcgcgcgcta atgctgagcg
    24481 tgaagccgct gccgtggttg ctgaagccca aagtaaagcg gccgaagtca tgcgcgaagc
    24541 ctccacgcgg gccgaatccg tccagcgtga ataccgcgcc caggccgagg ccgatattgc
    24601 caaagcccgc gaagcctcaa tggctgaagt ggagcaggag cgcaaccgca tgttgggcga
    24661 actgcgcggt caggtcgtca ccctggctat ggctgctgcc cagcgtgtca ttcaagattc
    24721 catggacgag aaacgccagc atgccctgct ggatgaattc ttctctggcg tcaagaatgg
    24781 caaagttgtg gtcctggaag gtgaatcgct taccggtcag gcggctgaag tggtcagcgc
    24841 gctgcccctg aatgaaaaag aacaggccac cgtcctgcag gaaatgaaat ccatgctggg
    24901 cggaacccct gccgtcaact tccgcgttga tccagccatt ctcggtggcc tggtggtgcg
    24961 cgtgggtgac cgcgtggtgg atggctcgtt tgccggtcaa ctggcgtccc tgcgccagaa
    25021 tctccaataa tcctgcgtgt gattctttca ggagtcgagg cttttgcccg ggcgccatgc
    25081 cccggtaaag gtctcgactc ttgctttata atgctcccct tttgctttga caggtgtgcc
    25141 catgcctctg ctctctgaat taatccgtca tcttcccgtc ccgccgctct cgcgcagcgg
    25201 ctcaaccgac ccggaaatta ccggcgtggt gttggattcg cgggcggctg tccccggcag
    25261 cctgtttgtg gcgcttgtcg gcggcagtgt ggatggtcac cgttacattc catcgaccat
    25321 tcagcgcggc gcggcagccg ctgcgggcac ccgccccgcg tctgagtttg ccgacctcgg
    25381 cgttccttat ttccaggtag ccgatgaccg cctctccctg gcttacctgg cagcggcatt
    25441 ttacggcttc ccggcccggc agcttaccgt cattggcgtc accggtaccg atggcaagac
    25501 taccacctcc aacctgatct accagattat gcaggctgcg ggaatccgca ccggcatgat
    25561 caccaccatc aatgccgtca ttggcgatac cgccctggat accggctttc acgtgaccac
    25621 gcccgaatcg cctgatgtgc agcgttacct ggcgcaaatg gttgctgccg gtctcaccca
    25681 tgtcattctg gagaccacct cgcacgggtt ggcccagcac cgggtggcag cctgtgacta
    25741 tgatatcggc gtggtcacca acattaccca cgaacacctg gactatcacg gctccctcga
    25801 agcctaccgc gccgccaaag cccgtctctt catcggtctc gcagaaacag ctcccaaacc
    25861 tcagggcaac ccccggttgg cgattctaaa cgccgacgac gacgtctatg actatctgcg
    25921 cgacctcacg cccgtccggc aaattgcgta tagccttagc gctggtgcgg ctgatcttta
    25981 cgccgccgac attgcctacg atgcctccgg cattcacttc actgcggtcg gccccggctt
    26041 tcagcagcct attttcagcc ctctggttgg ggccttcaat gtctccaact gcctggccgc
    26101 cattgctgcc acggttttgg gcctgggtgt tgctccatct caggcggctg ccggcattgc
    26161 cgccctgatg ggtgtgccgg gccgtatgga gcgccttgac atgggtcaga acttcaccgc
    26221 catagtcgat tttgcccaca cacccaatgc cctgcgccgt gccatcgagg ccgtccgccc
    26281 catgacctcg ggccgggtgc tggctgtctt tggatctgcc ggactgcgtg atcgtgaaaa
    26341 gcgccgcctg atggctgaag tctctgccga actggctgac ctgacctacc tgaccgccga
    26401 agacccgcgc accgaatcac tggatgagat cctcgccgaa atggtgggcg ccgcgtgcgc
    26461 ccgcggggcg gtggagggct caaccgtctt ccgcattcca gaccggggcg aagccatccg
    26521 ccgggcagtg caaaccgccc aacccggcga tgtggtcatc tgctgtggta aaggccacga
    26581 gcagtccatg tgttttggcg aagttgaata tccctgggat gatcgaattg ccctgcgggc
    26641 tgcccttgcc caacacctgg gcctgccggg cccacaaatg ccgcgcctgc ccaccaccct
    26701 gtcatagctg ttgatatcgc gtaacaaacc tcaaacgaac tggtattttt acacatttta
    26761 gggtatcctt taagtgctgg ggcgcaaatc gctgcccctt accgtttgat tttatattct
    26821 ccccaggagg acgccatgat aaaagaaacc atcaaagatg ctgaatcccg catgaagggc
    26881 gctctggata acctggagct ggatttttcg ggtatccgca ccggccgcgc cagccctgcg
    26941 ctggtggaac gcctgccgat agattattac ggcgctccca cgccgctcat gcagcttgcc
    27001 accatcagcg ttcctgagcc gcgcgctttg ctcattcgcc cctttgatcc ttctacattg
    27061 aaaacaattg aacgcgccat tctcgcctcg gatctgggca tcaatcccag caatgatggc
    27121 aaatccatcc gcctcaacct cccgcccctc agcgaagagc gccgccgcga cctggtgaaa
    27181 atggtccaca accgtatgga agaggcccgg gttgccattc gtaatattcg ccgggatgtg
    27241 atcaaagacc tgaaagaata tgaacacgaa aagctcatct cggaagatga acttaaaaaa
    27301 gccgaagaag atctccaaaa gttgaccgat cagatggtcc aacaggtgga atcgattggc
    27361 gaacgcaaga acaaggaggt catggaggtt tagccttctc ttatgacccc caaaaagcct
    27421 gctgaagcag tgcctgaact gcaaaaaatc ccccggcaca ttgccatcat catggatggc
    27481 aatggtcgtt gggctgccgc ccgcggcctg ccgcgtctgg ctgggcatcg ggccggcacc
    27541 gaaaacctgc gccgggtgct gcgcgcctgc gtggaatttg gcgttcccta tctgacgatc
    27601 tacgcctttt ccactgaaaa ctggggccgc cctgaagaag aagttcaggg ccttatgaat
    27661 attctgggcg aagtgatcga gcgcgagctg aatgaactgc accagcaggg tgttcgattg
    27721 cgtcacattg gccgcctcga gcagctttca gacaagctgc gcgccagtgt tgaacacgcc
    27781 atggaaatca cccgcaataa cgataaactc accctttgca tcgcctggaa ttacggcggg
    27841 cgagatgaaa ttgtctgcgc ggtgcgccgg atgatggctg aaggcatcca ggtggatgat
    27901 gttaatgaag aaacaatcag ccgacacctg ttcaccgcgg gcatcccaga ccccgatctg
    27961 atcattcgca cctcaggcga aatgcgcatc agcaacttcc tgatctggca agctgcctat
    28021 gccgaatggt acgtgacccc cgtcctctgg cccgactttg ataaagaaga attacgcaag
    28081 gccctgctgt cgtatcaaca gcgtgaccgc cgctacgggc ggctctcatc ttctgcggag
    28141 tgcctcccgg agaacggctc ggatgttgcg tgagcgtctg attgttatct tgatcttatt
    28201 gcccattggc gttgccctgg cagccttggg tggctggttt ttcacgattt tcgtggcggt
    28261 cattttaggt gttgccgcct gggaatatgc ccgtctgttt attggcggcg gctatcaacc
    28321 ctctcgcact ctgctgattg ctggcgtggc gcttctggcg gttgcccgtt acctctgggg
    28381 cgaatcgggc agcattcccc tgctggttgg tctgactctt ttggcaatgg gttatcacac
    28441 cattgcttac gctcgcggcc agcaaacctc agcctttgat tttgccatta ccgccgctgg
    28501 catcctgtat gttggctggc tgggcagcta tctggtcagc ctgcgcagct tatctggcgg
    28561 gttgtactgg gccctgctgg tcatcggttc cattgcgatt ggagatggct ttgcctatct
    28621 ggttggccac ctcctggggc ggcataaaat ggcacccaac ctcagccccg gcaaaacctg
    28681 ggagggctac atcggcggca tccttggcgc cattgctggc ggcgccctgc tgggctgggt
    28741 tttcggcctg atggttcccg aaatttcccc ccgcctgggt ctgctatttg gcctcattct
    28801 gggcgtcatt gccccccttg gtgactttgg cgagagtatg ctcaaacgcc agtttggcgt
    28861 caaagattcc ggacgccttt tgccgggcca tggcggcatt ttagaccgcc tcgactcctg
    28921 gttttgggct gctcccattg gtttttattt gatcctttgg ctggtttagc cacctgccat
    28981 acttccctcg attattgcca ttatccatat cacccaccat caggaatttg caggcgctga
    29041 cctgctttcc agaacatttg tgaccaggag gagtcaggga tgaatgagac caaaccaacc
    29101 cgtaatctgg cgcttgaatt ggcgcgcgtg accgaggcag ccgcgctggc tgccgggcgg
    29161 tacatgggcc gcggcgataa aatcgccgca gacaaggcgg cggtagatgc catgcggctg
    29221 gtgctcaaca cagtggatat ggatggcgtc attgtcatcg gcgagggcga aaaagacgaa
    29281 gcccccatgc tctataacgg cgaacatctg gggcttggag tgccgcccga ggttgatatc
    29341 gccgttgacc caattgatgg cacccgccca ttggccttcg gccgccacaa ttccatttcg
    29401 acggtggcgc tggcgccgcg cggggctatg tttaaccccg gcccctttgt ctatatggaa
    29461 aaaatcgcgg ttggccccga agccaaggat gtgattgata ttgaagcccc cgtatccgtc
    29521 aatctgcgcg ccattgcccg ctccaaaggc gcagatgtca acgacttgac ggtagtcatc
    29581 ctggaccgcc cgcgccacga atcattgatc gccgaagtgc ggcgaatggg cgcccgcatt
    29641 cgcctgatcc cggatggcga tgtggccggc gccttgatgg ctgccatgcc cgagaccggc
    29701 attgatgtct tgctgggcac cggtggcact cccgaaggtg tgctggctgc ggtggcgctg
    29761 cgctgcatgg gcggcaccat ccagggccgg ctggccccgc gttctcaaga cgaactggaa
    29821 cgcggcctgg ccatgggcta cgatgtcaag aaaatcctga ccattgacga cctggtcaac
    29881 tctgaagatg tcttcttctc tgccacgggc atcaccaatg gcgatttgct gcgcggcgtg
    29941 gactactttg gcgatggcgc cagcaccgac agcctggtca tgcgcggcct gaccggtacc
    30001 gtgcgccgca tcaccgccac ccaccgcctc ggcaaactct ctcgcatcag cgaaatcccc
    30061 tacgaataaa ccaaaagaac cgcccggaga acttctccgg gcggcctctt gtgaattggg
    30121 gtaatcttac ttgatgatgt aggtcaggct ggcatccgcc gtcacctgca tctgtccggc
    30181 agacattggc acgctggcat cagccgacat gtagccgccc ttgccttcgt acaccgggat
    30241 ggcgccgcct gagttgtaga cgctggcggt ttgcagttca cccagcgtga cgcctgccgc
    30301 agcagccagt tcctcagcct tcatccgggc atcagcaatg gctgcggtgc gggcctgggc
    30361 ttcagcttcg gtgcggtttt caacatcgaa ggaaatacca ttaatctggt tggcgcctgc
    30421 ccgaacaacg gcatccagga tctggcccag cttgtccagt tcgcgcactt tgacataaac
    30481 cgtattgtcc accgagaaga acttgcgcac aacctgaccc tcactgttgt attcctgatt
    30541 gggatacaca ttaaaagccg tggtctggat gtccttgggg tcaatcccaa actcgctcag
    30601 ggtattcgca atcgcctgag cctggctgtt attgccgttc agggcttcgg tcacggtgtc
    30661 gccatctgaa tggacgccaa tgttgatata ggcaatatcc ggaacaacat acacaacgga
    30721 atgaccgctg acgctgatag tgcggagttg agtctgccca cagcagttgt tggtattggt
    30781 agcgcccagg ccgctgcaag ctgtcagggt tgccagaaca gccagagcag ataccgcgat
    30841 aagcataaat ttggatttca ttttgtgcct ccttaggctt tgatagtgag atattggtgt
    30901 tcataagacg ccagcggctc tcaaaagttc ccgccatgat aggtcaattt cacccatttg
    30961 gcataaaaat ccgccccatg atctatgggg cggatttcag actgacagga aaatctaccg
    31021 aatcacttca atccgcacca ggccaaccgc ctgggtcaca tccagttcaa tcaactcgcc
    31081 atcgctgtct ggcgattcaa tcaatccctg gctgcgctcg taatcagacg gggcaaacac
    31141 cggagccagc ccgctgtgat tatggatccg cacattggcg ccttctggca cccggatgat
    31201 gatctcacct acggcgcatt ccaccgtacc ggtaaagcca tcatcatcag gcagggtcag
    31261 cacaatctgc ccaacggcaa tgctggcggt caggccttca atctgcaaac catccaggtt
    31321 caggcgctgc tctcctacag ccagttcggt gttcagcgtc acgggcagtg tgtcggtgag
    31381 tttgagatcc cagccgggtt gcgaaaggta attcatattc ggccagatgg tggtgcgccc
    31441 ttcagcgctc aagctgtagc gggccacgct gccgctggaa ttcaggtttt cattggcgtt
    31501 caacccctgc agaagcgcca ccttgccgtc aattaattga ccgctcgccg ccgggccgcc
    31561 aacaatcaac tgccccaccg gggattcaat tgatacattg gcttcctggg cgttcgctgg
    31621 cagcaccgcg ctgatagact gggtttgccc ggcgttttgc ggagtttgcg tcatgctcaa
    31681 ccacaaaatg ccgcccatca ccaggatcac cagcagcgcg cccagcacag tggcccacgc
    31741 cggtcgccct ttgacgacca gatcaacacc ccaggccacc aggaagaccg gccacagccg
    31801 cagcagcagc ccaaaggcat cccacggcag ccagcccagg ttccccagaa tcagtgccgt
    31861 acccagccca ataccaaccg ccgcgccaac aaagttttca cgcttgtaaa tgtcatccag
    31921 cccgccgatg ataaaaatca ctggccagta ggtggcaatc aggctgccaa aattggcatt
    31981 cagtacgccc agattactga gcaggaacag tgcaccgatc aagatcaacg ccagtggcca
    32041 gaaaatgccg cggcgctcaa ccttgtggtg ccgattaaca gaagtatctt cgctcatcgc
    32101 ttgctccatt caggattttg caggtcgcag ggcgcgccag gccaaaaagc cgccggccag
    32161 aatcagcccc acgccccaga gataattcat ccagtccatg gctgagaaca gtgccagtac
    32221 accgatgatt gccagaatgc cggccggcca ccacgcccac gtcttgcgtg tgcccatgga
    32281 cggcagcagg gctaccagtg caaaagtgat cgccagacca aacaggaaga tgcccgcttc
    32341 gtcaacctgc agaccttcca gcccggtcac tgcaaccagc gtggtcatca cccctgccgg
    32401 gatgattgcc caccagttag ccgtattcag caggtaaacc agccagaacg ccagagcaat
    32461 gccgcccaga acggccatgc cgctccaacg cccatccccc aggttgccga ggagaatggc
    32521 ggccccaatg cccaccagca cgcatccggg gattgctgcc caccaggcgc ttcgattggt
    32581 catcaggtgg aacagaaaag ccagcccacc cagcataaaa aatccgccaa tcaccagccc
    32641 gccgatgtcc aggatattca ggctgctcag caggaataat ccgcctgcca gtatcaagcc
    32701 cacaccggca aataaccgcc aggaatttgt tttcatcaca ttatcctttc aaaaattagc
    32761 gaatatcaaa cgaacccacg ccaccctcga tgtcaatatc gacccggttg gcggccatat
    32821 cataattggg cgagatgtac acatcgtcct ggcgggggaa gcgggcagta tccaccgtca
    32881 ccgatgataa acccgtctcg gcctttatcc gcgccgccac accctctggc acacgtaatt
    32941 taaccgtagc cgctccggct tcgacttcca catgcgtctg tccggcgcgg tctggcaggg
    33001 tgatctccgt ggagctagcc ccggtttcta atttgatctc cttgaccagc aggccggtca
    33061 gatccacccg ggtttcaccc gcgcccgtag aaagcttgat gtagagcggg atttgccggt
    33121 tgagccgcac atcccagtgc agcccctcgc cgcctaagaa caccggcccg gccacaaagt
    33181 tatctctagc tgagagtttg acgctgggaa tgctctgggt ggtgtccaca tgcgactcca
    33241 ccccgcccac aaacgtgccg cccaccagtt caatcgggtt atcgccggct gcgatctgca
    33301 gccgcccggc gccatgctca aacttgatcc gggcttcagt aatgccgctg tctagtggca
    33361 ggctcaggct ttcagtttcc aggctgccgg cctgattctg tcgcagtcgc gggcgcagca
    33421 gcacccacag gccaaccaga atcagcagtg acggccaaaa aatggtccag aaattgccgg
    33481 gcagaaaacc cacattctgc agcagcaaaa tgacgccaat cagcgccagt aaaattcccc
    33541 agaaaagacg atttgcacgc atggttctat tcctccaaaa aatgagacta actgatatac
    33601 gcagaatacc acattttgtc gcgttttaat ccacccaaat acccccgccc gctcctattt
    33661 tcacttgccc gtcttccaga cctgggtggt tatgcccaat tcttgaatcc attcgtcatc
    33721 tatcccgctt ttgtggtaaa ttaaaaacat gcgcaccggc cttgacctga ttgaaattga
    33781 ccgtctcgag cagttggatc ccagcatccg ccgccgtttt ctggcgcggg tttttacccc
    33841 cgccgaactg gcccaggcag gtgattcaaa ccccagcctg gcagggcggt ttgctgccaa
    33901 agaggccgtt gccaaggccc ttggaaccgg cattggtgtt gtgcactggc aggatattga
    33961 gattttgacc ctcgcaagcg gcgagccgca ggtccacctg cacggcgctg cccgcctcct
    34021 tgccagcgac ctgaagttga cggagtggtc cctcagcatt acccacaccc gcaatctggc
    34081 ggctgctttt gtggttgccc tggctgctcc ggagcctggc gcatgaagat tctcgccgtt
    34141 agcgatgttg aactgggttt catctataac ccccagattg cccagcgctt caaagatgtt
    34201 gatctggcga ttagctgcgg tgacctgccc tactattatc ttgaatttat catcagcatg
    34261 ctggatatcc ccctctatca tgtgcgcggc aatcacgcca gcaaggtcga aagcggcacc
    34321 tctggcgacc gtgccgaacc ctggggcgcc attgacctcc accgccgggc cgtgcgcgat
    34381 gaaacgggct tgctgttggc aggcattgaa ggttctttga ggtataacga tgggccaaac
    34441 caatacaccc agggagagat gtggctgatg tgctgcctgt tgacccccca attattcctc
    34501 aaccgagttc gccatggccg ctatctagat gttctggtca cccatgcgcc gcctttcggc
    34561 atccacgacc tggatgatcg cccgcatcag ggcataaaag ccttcaactg gttgaaccgc
    34621 gtcttccagc ccacagtcca tctgcatgga catatccacg tttatcgcca gcaaacggta
    34681 actgaaaccc aggtcaacca aacctgggtc attaacacct tcggctaccg cgagttcaaa
    34741 ctccaactgc ctgctgccaa ccagcgcggg cgggccttat atcacattga agggaaacgg
    34801 gtaaagtcat gacacctacc gatcctccgg gtgtttttgg cccgggccgt ctagcaaaag
    34861 aagactttga cgccgccctg cgtaaagggt tctggcggtc ggttttttcc tggctgcggc
    34921 ggtcagataa tcagttgctg ccgtttgatg aattccgccg ccatttgccg atgatgggcc
    34981 aatctgatat tggcatgcgc caaattccca ttgatcgcat tgttggctca gtaggccgct
    35041 accgcgattt tgacggcgct ttcttgcccc gcactgagcg cagccgcccg cgctgggaat
    35101 ccattgaccg ggcgcacatt caacaggtgc ccttgccccc aattgaagtg tataaaatcg
    35161 gcgaaatcta cttcgtcaaa gatggcaacc atcgcgtctc agtcgctcga gagcgtggtc
    35221 aggtctatat tgatgccaat gtgattgaaa ttgtggtgcc cttcccggtg gacgagaaaa
    35281 ccgatattaa tgccctcata ttgcaatggg aactatccca attcctggaa aagaccgggt
    35341 taaaatccct gcgccccaat gccgatatcc gtctttcgat gccggggctg tacaaccgcc
    35401 tgttggatca cattcagacc catggctatt ttatggctca ggattaccag cgtgagattg
    35461 cctgggaaga ggccgtcaca agctggtatg acgatgtata ctggcccctc gtcagtgtca
    35521 ttcgccagca aaatatcctc tccgacttcc ctggccgcac tgaggccgat ctttatctct
    35581 ggattatcga gcatctctgg tacctgcggc aggaatttga ggatatcagc ctggaagacg
    35641 ctgccttaca tttcacggcc tcttatgcgg ctcgcccggg agcgcaccca ttggagaaga
    35701 ttttaggcgg tgtccacagt ctggctgtgg atgatggtga aaaaacgccg cccacaccgg
    35761 gcggcgctga agggatttga acccgcgacc tcggccttga cagggccgtc aggccgcttc
    35821 ttgaacccca atccatagat ggttaggtaa aatcatccga ttgctgcgca cctctacccc
    35881 gcggggcgga ggtccattct caggcagcat actgggatgc acaaaacaga gattgggctg
    35941 ctgtccatat ttggctttgt aataggttgt ggctgccgaa atcttggctg ccagatccag
    36001 cttcgggtca ttatcaaacc ataacatacc tacgttcatc gggttacctc caggttgctt
    36061 gtcttatgcc aggggagcct gaaatgcgag ccagctcccg ccaatttgaa ccgaaaactt
    36121 acccacgccg gtcatcgctc gctgcggtcc ttcatccgat tgtgccagtc ggcgggctgt
    36181 actgtgggcc tccacccgtc ccagcaatcg ggtgcgaaaa ccagtcatca accggcccag
    36241 cgagagcgat tcctccgggc tgacggttgc tatcgtccac acgcccatgg ctgggccctc
    36301 tcgcagcatc caatcaaaat agatctgggg atcatcgtcc cagcgtttca cctgttccag
    36361 accatccagc agtagaacct gggccgcacc cagttctcgc ccccaccgcc gctgctcagc
    36421 gatagccgcc agttctacaa cctggtcacc agctgcccgt tcccaggtgg ggtatacccc
    36481 cccatatccc ccccattctt cgggtttagg tgacagaatg ctcacctgaa catcataacg
    36541 agaattattc cgtgctaatg agttggcgac cactttcaat agattagtct tcccacagcc
    36601 attttcagca gtcaccagca gcggtcccgg ccgtgggtcg ttcaagtcaa acaaaaccgg
    36661 gtatccatcc tctccctgtc ccagcacagc tgtttgaacc ggcaaatacg gaaaatccac
    36721 cagaatatca tccagtgtca gagtggcagg ggttttttct ggtgccacaa ccagcgaatt
    36781 aagggttcga gtcagttgca tgttcttttg tcctataaga ggaatagaac tagtgttcta
    36841 tcggttctat tatagcacat tagaacataa tttcaatcat ttgcggcgaa aacaatctta
    36901 aaatttcgga tattttctta gtgccgttca atccgaaaac agctcatccg catatcttcg
    36961 ttgcctccaa tgatgtagcc acccatcgga tcaatcgcca gcgagaaaag gtatttttca
    37021 acctcgatca tcgccagttc ttttcggctt ttcagatccc ataatttaac cttcaaatca
    37081 gcacaggtga ccgcgaactt gaggtcctgg ttgacggctg cggcagtcat tcgccccagg
    37141 gtggtctcaa atgcgccacc aacttgcccg gtttttggat cgaagaatcc ccccgtcgtg
    37201 tacaacatct cctgatctgg taaaacagcc cagttcgtgt ctacccgata gccaaaccca
    37261 tactgcttct ttttcccgtc cagaaggtca acaatatcca cttgattgta tttgagccca
    37321 ctcacctgca caaaccagtg accaaccact gcatgagctg caccggtacc ataccattga
    37381 cttcgttctg tgctggcatc aagattccat aaataccaat caaatgcatc ataattaatg
    37441 tactgcggat tgatctttgc cactccagtt aatgcccagt ttaatctcgg gtccagatac
    37501 accttgtgat atctggaatc tggcggctgg tattgcagca gctttcggat gagcgctcca
    37561 gaacgaaggt cccattcaat gatactgcca tcccccgcac ctgaaaccaa ccgggtgccc
    37621 tttgcaccgg tgctgatcgc catcacctgt ccggcatgtc ccccctgcat cacgccaaga
    37681 catcgcccgg tggtcaaatc ccaatggcgc acagtctggt catttgcgcc agttaataag
    37741 cgcttgccat cctccaggta ggccagcaca ttcacattgc cctggtgccc ttccaaccgg
    37801 gtacggattt ctcctgtcgc caggtctcgc actgccagcg tcgcgcggtc acatccatac
    37861 ggtgtgatca caattccacc ctgtgacggt atggataatg gatcgggtgt tccctgggtc
    37921 ttaagtgtac agaaaatctg ctcccccgtg tcaatccgcc attttctcag gcttgtatcc
    37981 cctccccccg ataacaagaa tgccccatct ggcgagacgg ccaattgact gatcgcacct
    38041 gtatcatgcc ctcccagcac ccgctccaat atccctgaag gccagcgcca gatgcaaata
    38101 ctgccatcat aatgccccga tatcagacgg cttccatccg gcgtgaatgc cagtgccgtg
    38161 gatctgctgc tccgcgctgg cagatccggc tcctccaact ggtcagtttc ccagttccag
    38221 caattaatct taaatcccat tccaaccaga atctgtttcg gattaggacc tagcaccagc
    38281 ggcacataca gatcattgat ggcttctcgc tgccagatga tctctccccg atccaggtcc
    38341 catcccacca tcaagcaccc atagcggcag aatccatacc tgccatccgg cgaaataact
    38401 ggcgcagagc gataataggc attactcccg gccttcccgg caggtcttgg cagggtcaca
    38461 ccggggcagt gattctcaaa atcccaaacc cgcatttcgc catcattgac taatgcccgc
    38521 cgtccatttg ccgcaacact gacatactcc cccgatggtc tcctccgctg acaccaggcc
    38581 tgtacctggc ttggatctgc tgaggggatt cccaccacgc cgccggtttc tcccaccacc
    38641 actgcacagg cgccatccgg ggttaggtac atccaatgaa ccttgtcata cccagcccag
    38701 ttccctctgt ccacgcgctg cccggtttca aaatcccaga tgatcaattc gcggtattga
    38761 tagttcaatg acaacgcaaa ccggccgtct ggggtgatgg ccatacacag aatgccaaaa
    38821 cttctatgcc caagtctggg ctcctgctca atcggttggg ctgactgcca ggccggcact
    38881 aacctgaaac tttgggcacc acggtttcgg ctgaaaatat tcatggtttt tcaccgtttg
    38941 aaattagaag ggggggatct caaatccaga agcagatgcg cttagtatag cataaacgat
    39001 tccagccaag cgccacaacc cggcactttc aatttcttga aatgccgggt tgaacagcct
    39061 gtctcctatt ttggggggct ctaaatcatg aaatcttccc cccgccagac cccgccttct
    39121 ggagcatgca cctgggcctg cggctgcggt ccgccgccat tcccattcca accgccgtgg
    39181 gcttcgcggg catcgctcac atgaaaatgc agccggttct cgatggcctc caaccgccgc
    39241 atcaaagcgc caaccgaatc ccccagggta tcgggcaggc gcccatgctc caggtcaggc
    39301 gcttcactgc gcgggcggct gcgcaccacc acctgccccg gcacacccac aatcaccgag
    39361 tttgctggcg ccgctttgac caccaccgcg ttcgcgccaa tccggctgtt gtcgccaatg
    39421 cggatattgc caagcacttt agcgccggcc ccaatcacca cgttatcgcc aattgtggga
    39481 tgccgcttgc ctttctccag gctcacaccc cccagcgtca cgccgtgata cagcgtcaca
    39541 ttttgcccga tctcggtcgt ctcaccgatg accacgccca tgccgtggtc aatgaagaac
    39601 cccggcccaa tctgcgctcc cggatgaatt tcaaccccgg tgaaaaagcg catgatttgc
    39661 gagaccatcc gcccggccag cttgaaattt cgcacccaca acgcgtgggc aattcggtgc
    39721 ccccagactg catgcagccc ggcatagttc aacaccactt ccagcttgct gcgcgcggcc
    39781 gggtcccgtt ccatcaccgc ccgaatatca gatttgagca ttttgaatag attcatgtcg
    39841 tcactctacc aggtcggcat acaaagccgt ggacagatag cgctccccaa acgatgggat
    39901 gatcacaacg atcagcttgc ctgcattttc aggccggttt gccagttgca ccgcggccca
    39961 ggttgcagcc ccggaagaga tccccaccag cagcccctcc tgctgagcca tggcccgcgc
    40021 tgtcttaaag gcatcttcac cctgcaccgg gatgatttca tcatagatcc cggtgttcaa
    40081 gatttttggc acaaacccgg cgccgattcc ctgcagcggg tgcgggcctt tggccccgcc
    40141 cgccagcact gatgacgcag cgggttcaac cgccaccacc tgcaggcctg gtttgcgcgc
    40201 cttcagcact tccccaatcc ctgtaatggt tcctcccgtt ccgactcccg ccaccaggat
    40261 atccacctgc ccatccgtat cccgccagat ttcctcggcg gtggtgcggc ggtgaatttc
    40321 cgggttggcc ggattgtcaa actgccccag gatcaaatag cgcgggtcgc tcgccgccag
    40381 ttcctgcgct ttgcgaatcg ccccggccat accttcgctg ccgggcgtca gaatcaactc
    40441 ggctccatag gcccgcagca gcatccgccg ctcccggctc atcgtttccg gcatcaccag
    40501 cgcgcagcga tatccccgcg ccgcagccac aaacgccagc gcaatcccgg tgttgccgct
    40561 cgtcgcctcc agtagaatcg tctccggccc gatcaacccc gcctgctcag ccgcatcaac
    40621 catcgccacc ccaatgcggt ccttcacact gtgcgccgga ttgaaatatt ccagctttgc
    40681 tgccacggtt gccgggctgc cggctgtcag ccggcgcagc cgcaccaggg gagtattgcc
    40741 aatcagttcc gtaatatcat ttgctatttt cattttcaat ctccatcaaa agacaatcta
    40801 ttaattaaaa aggccagcag aatcaaaagc agcgaactgc tttccagcat ccacatattg
    40861 gagtgcgggg tcgctgtccg ctgcctgatc tgctgacctt tggcaagctt tgtcaggctg
    40921 ggactatggc gcgccccgcc tctgacacat gcaagtattg gatctaatta tgactaattt
    40981 tgtcatcatg cccatcatta tagcgcacaa aattcgcttg tcaaggcagt ttgtccaggg
    41041 ttttatatac cctttgatag acccatccta taaaaacaaa ccagttcacg ttctgatgac
    41101 attaataacc aggccgggtt tgctcacctc aaaatcgcca ggcttcaggc caggcatagg
    41161 caagcgccag cagggctcca attgccccgg cataataata aaaccacggc ttcttgaacc
    41221 ggaacagcaa aaatccacag atcaccacga ctgcaagcat gatggtcacc gataaaaacg
    41281 gtagttcgga caaaaagaaa ccagttgtta taattgaagc cgccaacggg ccggtcagga
    41341 aaatcgcttt aagtaaatcc ggcagctttg tgcgtagcaa aatcagatat aacccaacca
    41401 gcgcaatggt gcaccccatg cgcagtgagc cggcaggaaa accttgtggg ttacttgaga
    41461 aaaatataga ctctagcagc aggtaaatac ccagatacag tgccagggtg agcgcaatca
    41521 tgattgcact gccaacaggt atcaccagca gaggcagcca aagcgtacgc catctgcttt
    41581 ttttaatcaa aatgtccaac cctttaagtt ggtttgcaga ttatatgccg tcaaatccaa
    41641 tcccagcggc gtaatcgaga catacccgtt tgccagcgcc ccaatatcgg tgccctcatc
    41701 cggca
//
LOCUS       DAOPVS010000007        40513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_911_length_40513_cov_57.050447, whole genome shotgun sequence.
ACCESSION   DAOPVS010000007 DAOPVS010000000
VERSION     DAOPVS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 40513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40513
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_40513_cov_57.050447"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1002
                     /locus_tag="PKL11_01595"
     CDS             31..1002
                     /locus_tag="PKL11_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ12319.1"
                     /translation="MTTPETEITPRPQRKISILRLFGTLLSIGLLIWLIEREGWDAIK
                     SAVTEISWWNLVLVLFLTFVSRFAVTGRWHVLLRSGGVPIRLRESIRLVFAGLFASNF
                     LPTTVGGDLIRFAGAVRLHYDAAICAASLVMDRLVGMVGMLTVFPIGVARLLSMPLPP
                     LPAGGYSGGLAMAVSLTGIWKWVWSKVQKLFRSTMHALRLWATHPRDLFSAYLFTLIH
                     MAALFATVWLILHDLGQPINFWQVAGLWSISYLVTLLPISINGLGVQELSLTFLFTNY
                     GGLSVESVAVMAILIRLLQMAASLPGAIFVPALIQPEAPPAAPETPA"
     gene            999..2933
                     /locus_tag="PKL11_01600"
     CDS             999..2933
                     /locus_tag="PKL11_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12320.1"
                     /translation="MKLPRPIWIARLNLLVSVILSILTLLELEKLGYGSGELLGHYAL
                     PRLIMLAFFATVGLVCLILLALTWRLRMADTLLRILQKLPRAKALNIVALVLGALVLG
                     WLAFRYDNGKFSTPVIRLWLIWFTAIFGAFFIQRLKPMPYALALASALLIAGAWFQIL
                     TFLPGLTDHPLSLGWSEGSRFYYGSLFFARQIYGEVLPIPVLHPARYLMQSLPFAFPS
                     LPILAHRIWQVILWVVCNAAAAWLLVRRLRLNSRWLAWMAACFAFLFFFQGPVYYHLI
                     LCTLPVLAWFDSRRPWRTLVVVLLSSLWAGLCRVNWYVVPGVLAATLYLLEQPQPRFS
                     WRYWVWPALHAILGTAAALLFSTFYKSISGNSPEVFGSAFDSPLLWNRLFPNTTYPLG
                     MLLGTAIAVLPFLLIILWSFWANRRAWHPLRLLGLGAILFVFLAGGEVVSLKIGGGSN
                     LHNLDAFLVFQVVIGLYLIFNRFVPDAHPIPAAKLQPAWWLAGLTIVIPFFQMIGGGQ
                     PLHLPDRTKVSDDLSQLQVMLNQADGPVLFLTERHLLSFKTVTAADFEPEYEKVFLME
                     MVMSGNQTYLDHFYSDLQQHRFALIVSEPMNMNMQDERFAFAEENNYWVQRVEQPVSQ
                     YYRILDEFNDSGFHVWVPVE"
     gene            2936..4909
                     /locus_tag="PKL11_01605"
     CDS             2936..4909
                     /locus_tag="PKL11_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12321.1"
                     /translation="MLDLLVLGIWALFWGLGGIWLAAAAFRLTRSEQLLVGLVLGLTL
                     ETLLANILGRFILPAPAFLIAAGLTFLLGAIFIVFKNGWRALYHFPLDWRAALALLVI
                     TGLFTQIGRGLAIFDDYAHLPTTSLLAAGSLPLKFALNPDAPYTYHYFLMLFAAQITR
                     LGDLAVWFALDLSRAFAFGLTIVLAFIWSRRITRGIPAGILGALTVLFAGGSRWLALL
                     FPGGWLENASAQMTMVGSGSASGSTFATALTGPWAIEGGASLDFPFAFVNGVLQPGVL
                     SMLGPNSTFLLALTFTLLLTYNRWRSWVGAIPVVIWVAAFSLLTESGILLGLAGWGLV
                     LVAVLIKQRNWRIPAALLTWLLAWLTGTLLGAFQGGALAQIILDAFGKSSAAAYQTVG
                     FQFAWPPILVSTHLGALDLSQPATLLLAIIEAGPLIFILPLTMFWGWKALRAGRWYEA
                     ALITSGLLSLGVIFVQFAGSTGIRNTARLYEFMTLSLVYAVPLGWLWAQRQGWLRKTI
                     ALSALCIASLGGLVVFGVELTAMPSPTQSYYLNDLDAVMMSRYWDKLEPDALVFDPLP
                     NRSPVLFARYTNAAQTWYAYKPEWLALKTNPNPVALHAAGYDYLYLDSTYWEELSPTV
                     QVSISDGCAQQIDQIEDWRHDTRWLYDLRACGE"
     gene            4988..6766
                     /locus_tag="PKL11_01610"
     CDS             4988..6766
                     /locus_tag="PKL11_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12322.1"
                     /translation="MNIAEFRIAKEYRTDQRGPVRWVFSHAIRQWPLLLMALFGAVGN
                     AALAAVLPILMGEAFNLVLSGSPDLTRLGQIAITLAISQLIRGVIQFSRNFGFEWIAQ
                     RVERDVRDELYINLLGKSMTFHNLQSVGDTMSRATNDVREVNYLFSPGMAQVLGSLNF
                     LIMPLIFAPRYHPTLILTPLIFVILYYFALRNYLGQLRPVTDRVRATFGQLNTRLSEA
                     LDGIETVKGSAQEDAEVARFSANASSYRSAVVRQGDVEARFIPLLLLTLALGFGLLHA
                     LILFRQGILDVGQVVAYFGLLLMLDFPTFASMWAYSRISLGLAGARRILDLMHRETYL
                     DENVSGHQAQMRGEVEFEEVCFAYPQQNNAETDACDDGILQHISFRVKPGQTVAIVGQ
                     TGTGKTTLVRLINRIYDSTSGTVRVDGLDVRDWNLAVLRQQISIIEQDVFLFSRTIAE
                     NIAFGKPDATQEQIEAAARAAQAHEFITGFSEGYQTAVGERGVTLSGGQRQRIALARA
                     FLTDPRILILDDSTSAIDSATEDKIQRAIYTAARGRTTFIITHRLSQIRWADLILVLR
                     RGQLVATGSHDELMKTSAAYRRIFSE"
     gene            6776..8611
                     /locus_tag="PKL11_01615"
     CDS             6776..8611
                     /locus_tag="PKL11_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12323.1"
                     /translation="MGFWSGLNAEHYDRKYSDRELFRRILAQFRPQRARMGWIVVTVL
                     ILAAAGAANPLVVSRGVDLLKDQISGWTMAAISGAVILLGGISWLANYAQRRLAVRAV
                     SDVVLSLATNAFDAAARHDMSFHDEVSSGKIVSRITSDTQDFGQLVMLTTDVIAQLFE
                     ALVLGVILLVIEWRLSLYIFALIPVVFVFAIAYRRLARRVTREGMRAMANVNSTIKET
                     ISGMAVAKNFRQEASVYRDFSTANLASYQVNFRRGLALSFVFPTLNTLGGIMSALLIY
                     VGGLSVADGLVTAGAWYLFLSSLDRFLFPVMNISSFWTQIQNGLSASERVFGLIDASP
                     AVVQTDSQIAPHLKGEIIFDHVSFRYKETEPVLENFNLRIAPGENLALVGHTGAGKSS
                     VAKLTARLYEFQQGRILIDGLDIRSFDLNSYRRQLGIVSQIPFLFSGTVADNIRYANP
                     TISDSVIEDLARQIGDGEWLEALPDGLQTDVGERGARLSMGQRQLVSLMRVLAQRPAI
                     FILDEATASIDPFTEWQIQQALKLILAQSTSILIAHRLSTVRSADRILVMEQGQIIEE
                     GSHNSLLQRGGHYATLYNTYFRHQSLAYVEQAQEMRSALTTEPTP"
     gene            8608..9468
                     /locus_tag="PKL11_01620"
     CDS             8608..9468
                     /locus_tag="PKL11_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ12324.1"
                     /translation="MNAAYYKNLGDQYAAANKLEEALRAYERALELDANDPDLYFARA
                     EVYRRLGRLDPSLEDYNQGIELSPLRPEGFIGRGNVYLAWRYYDRAIQEFGRAIALLP
                     NGADGYYYRGLAYLQLQKFQFALTDLNRALQLKPDYLEAQVQRGLTYHAIGDFQNALR
                     DFTTVLALDARYALAHYYLAQTYKKLGRDELAVGSLVTFQSLVQSSTWQQKARQELEE
                     LRKHAGTPRSTQPLRAVQLNPADRAEVLRLLRMGDKVAALKRVAEASGAGLRASKEYV
                     DELIRTELRS"
     gene            complement(9469..10326)
                     /locus_tag="PKL11_01625"
     CDS             complement(9469..10326)
                     /locus_tag="PKL11_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ12325.1"
                     /translation="MSKVAIVTDSTATIPAELMRGYPVQVVPLQVIWGDETFLDGLDI
                     QPADFYQRLKHTKVMPTTSQPSPAAFIKVYQKLLDEGYSILSVHISAALSGTVDSALQ
                     AKAHFTNAPIEVVNSQLTSMAMGFPVMLAARAAAQNATLQECRVLVERATEQSGVCFV
                     VNTLEFLHRGGRIGGAAAFLGTALNLKPLLELREGRIEAIERVRTMGKAVDRLIALTE
                     ERIGTRTPIRLAALHAHAPAEARELLARARERFGLTDVSETFIADISPVLGTHTGPGA
                     MGLAYMAGM"
     gene            10492..11814
                     /gene="ffh"
                     /locus_tag="PKL11_01630"
     CDS             10492..11814
                     /gene="ffh"
                     /locus_tag="PKL11_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNZ12326.1"
                     /translation="MFENLTQRLNQIFQNLRRHGKLSAEDVDAAMREVRMALLEADVH
                     YSVVKDFVARVRERAVGHEVSKALNPAQQVIKIVHEELIATLGEPARLNLTGAKPRVI
                     MLVGLQGSGKTTHSAKLARQLREQGERVMLVAADPYRPAAIRQLQTLGERIGVPVFTE
                     NGVKVPDLAAHALDQAQKGGYSVLLIDTAGRSQLDQDLMDELRAIHKRVNASEILLVV
                     DAMIGQEALHVAEGFRDTVAITGLILTKMDGDARGGAAISIRGVTGVPIKFLGTGEGL
                     EALETYDSGRLASRILGMGDVIGLIERAEAAMDAESAQDQAERMLSGQFTLEDFITQL
                     RQIRKMGPIHQILEMIPGSMGQMARTIDPRDAEHQMKQTEAIISSMTVQERRHPDILN
                     ASRRRRIARGCGMDVQDVNRLMKQFREVQRMMKTLQKTGGRGMPRLFG"
     gene            11921..12274
                     /gene="rpsP"
                     /locus_tag="PKL11_01635"
     CDS             11921..12274
                     /gene="rpsP"
                     /locus_tag="PKL11_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNZ12327.1"
                     /translation="MVRIRLRRVGLSGQISYRIVVADKEAPRDGRFLEIIGHYNPRTE
                     PFTFDVDEGRVYDWMNKGAQPSESVQMLFRSVGLLDRYARLKAGESLDVLLAEAKAYY
                     EARKVNTSTTAAQKK"
     gene            12281..12517
                     /locus_tag="PKL11_01640"
     CDS             12281..12517
                     /locus_tag="PKL11_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNZ12328.1"
                     /translation="MKALIEYIACSLVDDPKQVSVQQGRDGDKVRIELRVAKEDMGRV
                     IGKSGRVANAMRVLLRVAAAREGKQVTLDVAEPR"
     gene            12514..13077
                     /gene="rimM"
                     /locus_tag="PKL11_01645"
     CDS             12514..13077
                     /gene="rimM"
                     /locus_tag="PKL11_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNZ12329.1"
                     /translation="MRQPNHSAAQSPDQETGSPQSGGPVFLAVGQLHRPHGVRGEIEM
                     EILTDFPERLVPGKVLYVGEKHARHRISSVRPKGTGLLVGLEGYTDRDQVAVFRNLTL
                     YVRSDELPSLPPGQYYHHQLLGLQVVAEFGESLGRLTEIIETGANEVYVVTDAAGQEQ
                     LLPAIESVILGISLERGEIRVRPQEWE"
     gene            13082..>14166
                     /gene="dprA"
                     /locus_tag="PKL11_01650"
     CDS             13082..>14166
                     /gene="dprA"
                     /locus_tag="PKL11_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ12330.1"
                     /translation="MDERAYWLGFNLIKGIGAVRLRALLDFFGDLQTAWNAPLEALTA
                     AGLSERLATSALAARKEVDLEARWNELERKQITLLTWNDPGYPRRLKEIDQPPPVLYL
                     RGDLLIEDDWAVAIVGTRRVTSYGRQVAEELAAYLARSGIAVVSGMARGVDGVAHDTA
                     LRAGGRTLAVLGCGVDVTYPPEHLRLSERIIAQGALISDYAPGTQPESANFPPRNRII
                     AGLSLATVVIEAGETSGALITASFAADQGREVLALPGNIYAPQSKGTNKLIQTGARPL
                     LRVEDVMDALSLDRVQEQRQARQILPADDLEARLLDILSAEPQHIDEIGAQIDLSIEQ
                     VSATLTLMELKGMIRHVGGMNYVTTRET"
     assembly_gap    14167..14266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14336..15442
                     /locus_tag="PKL11_01655"
     CDS             14336..15442
                     /locus_tag="PKL11_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ12331.1"
                     /translation="MPETSNQSTYAIIMAGGGGTRLWPLSRQSHPKQTLQLISDRSLF
                     QLACDRLQGFLPPEQILVVTTADLAVTLQAQAPDIPADNYLLEPMPRGTASVVGLAAA
                     VLQARCPSARMCVLTADHYIADISRFQSVMQAALTAADQGWLVTLGIPPTFPATGYGY
                     IQRGQPLGNFNGQLIHRIQRFKEKPDLETAQSMCASGGYDWNSGMFFWRVDRILVEFA
                     RWMPDLSAILAQLTVAWGSPEYDQRLRELWPTIQPQTIDYGIMERAEQTAVVPAQGIG
                     WNDVGSWESLFEALPGDAHGNIILSTASHLELEDQNSLIYANTPDRLVVTVGLENMIV
                     VDTPQALLVCPRHDAQKVRQVITLLKQNGMERYL"
     gene            15459..17765
                     /gene="topA"
                     /locus_tag="PKL11_01660"
     CDS             15459..17765
                     /gene="topA"
                     /locus_tag="PKL11_01660"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNZ12332.1"
                     /translation="MEAYCVKCKTKREIVDPTPAFNRVGSPVTRGTCPECGTKLFRTG
                     RTPEHEGLAPAEHQVQPRSGKLVIVESPAKARTVGRFLGKGYTVRASVGHVRDLLRSE
                     LSVDVEQGFRPKYRVPNEKRQVVRELKELAHHAAEIYLATDPDREGEAIAWHLLEAAA
                     IDPQITRRVVFHEITEGAISEAFSHPREIDMDLVNAQQARRILDRLVGYSISPLLWEK
                     VRSRLSAGRVQSVALRLIVEREREIEAFIPQEYWTIEAEFKPEGGKSTYTARLAKVDE
                     QDPVLNSESVVQPLVKDMEAAAYQIDRIKKSERRRKPSAPFITSTMQQEASRKLGYTA
                     RRTMSIAQQLYEGLDVGEGGSTGLITYMRTDSTNVAEVAINEVRQFIQSNYGADFLPA
                     TPPQYKTRAASAQEAHEAVRPTSVLRSPDKIKAYLSPEQFKLYQLVWQRFVASQMEAA
                     VYDTVSIEIGALTPAHRYLLRASGQTLRFPGYLVVYEEAKEEDQKPEEEEANVRIPPG
                     LEEGQKQKLVRLIPEQHFTQPPPRYSEATLVQVLEEFGIGRPSTYAPILSTIQERGYV
                     IREAKRLFPTETGCLVSDLMVEHFPNIVDMHFTAHMESDLDDVAAGNREWADVIGEFY
                     RDFEPTLERARSEIPITKAELEKIGRACPECGKDLVIRWGRYGKFISCSGFPACRHTE
                     AFLVKINVTCPEDGGDIVERKTRKGRMFYGCANYPNCTFTSWKRPIPVPCPKCGGTLV
                     IANKREMQCIKCQETFLVEEVPAAAESA"
     gene            17913..18950
                     /locus_tag="PKL11_01665"
     CDS             17913..18950
                     /locus_tag="PKL11_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12333.1"
                     /translation="MEKIKSLLNNPLYAGIVGLIIGLIIGLPLLGWGLWPVKYYDAAP
                     VNLRSDLQTDYLCMVIDAYSQDTTQVAIAQKRYAELGEVGPTLLSTLTAATCGVGVSD
                     ADIATFRTIVGAAAPVETTVPVTGETAAPIVTPEPTKKPTSFGWVIALCLGTLVVLGI
                     LAYLLILKPRKRSTSGPSVVEQAQEAARSAVQTDFVAQGHEPPVAQFMTTYMIGDDLY
                     DDSFSIDSPTGEFLGECGVGISESIGVGDPKKVTAFEVWLFDKNDISTVTKVLMSAHA
                     FDDENTRSMLAAKGEPILVEPGRRVQLETKTLILEARVVDTNYGQSALPAESFFDRLT
                     LELAIWPKPHA"
     gene            complement(19010..19477)
                     /gene="greA"
                     /locus_tag="PKL11_01670"
     CDS             complement(19010..19477)
                     /gene="greA"
                     /locus_tag="PKL11_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNZ12334.1"
                     /translation="MAEVHYLTADGLEKLKQELEQLKGPARQALSERLRFAIQQGDLS
                     ENADYIAAKEEQGFLEGRIIELEQILQNAAIIDESRKTDGVITIGARITIQEDDFTPE
                     TYQLVGPTEANPAKGRISHESPIGRALLGQRLGDTVSVDTPSGAIKFKVLKIE"
     gene            complement(19536..20687)
                     /locus_tag="PKL11_01675"
     CDS             complement(19536..20687)
                     /locus_tag="PKL11_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNZ12335.1"
                     /translation="MIFSREQLEQLEDQTLAPYGAHSSKTRGRLYPDHEPEFRTCFQR
                     DRDRVLHTTAFRRLEYKTQVFINYEGDYYRTRLTHTLEVAQIGRTLARALGGNEDLTE
                     TICLAHDLGHPPFGHSGESTLARLMRDHGGFEHNKHSLRIVTELENRYPDFPGLNLSW
                     EVLEGIVKHETEYDKSDARAFNPNLRGNLEAQIANVSDELAYTAHDLDDGLRSKMLTP
                     EMLEGIEWWEIVSKEIGWRGGALSAQDRHAIIRNIIGLGVSNIVSTTDKRLREHSIRS
                     MEDLQQMPFNLVGHDEDMHRRNRQLKDFLYNNLYRHYRVVRMAVKAERVLSELFNAYR
                     DEPSTMPKHVQDLIEARGLERTICDYIAGMTDRYAVEEYHKLFDPSMLP"
     gene            complement(20671..21498)
                     /locus_tag="PKL11_01680"
     CDS             complement(20671..21498)
                     /locus_tag="PKL11_01680"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNZ12336.1"
                     /translation="MKELVFLKLGGSLITDKAHPNTLRRGTLPRLAREIAVVCRQNPD
                     IQIIIGHGSGSFGHHAAKNHKTMDGVRTGEAWQGFAEVWLAARTLNQKVVEALTKAQL
                     PVITFPGSAGITANQREIVSWDIGPLQAALDAGLIPVVAGDVIFDQAMGGTILSTEDQ
                     FYYLAQKLHPDRILIAGVEPGVWADYPDCTRIMDLITPQTHPALTAALQGSASADVTG
                     GMASKVERMIQLVTEEPYVKICIFSGLQRGLIEQALAGNLPGTLIFNPSGGPDDFQP"
     gene            complement(21495..22457)
                     /gene="mvk"
                     /locus_tag="PKL11_01685"
     CDS             complement(21495..22457)
                     /gene="mvk"
                     /locus_tag="PKL11_01685"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNZ12337.1"
                     /translation="MPATTATAPGKCILIGEHAAVYARPAIAAPVTDVAVRATISARP
                     NAPSGEIKIEAPAVGLDARLSELPDDQPIAWIIHLVQRAIQVKRLPAMQIKITSTIPP
                     AAGLGSSAAVAVALIRALTQFLGYPLDEMTVCQLAYESEKRQHGSPSGVDNTVITYAR
                     PIYFVKGQPFEALNVAEPVHLLIADSGQPSSTAEVVADVRRGWEKSPAAFDAIFDAIG
                     SLAQQARLCIENGPAKSLGALLNQNHQLLQQLGVSNPKLDRLVEAAIEAGALGGKLSG
                     GGRGGNLIAQVEEAAADHVTNALLKTGAVRVISTTIHPNRNNLL"
     gene            complement(22465..23337)
                     /locus_tag="PKL11_01690"
     CDS             complement(22465..23337)
                     /locus_tag="PKL11_01690"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNZ12338.1"
                     /translation="MIRALIKAMRLKQWPKNGFVLAALIFDVQFLVLPSVLRSVAGML
                     LFCLLSSAVYLINDIFDIKADQQHPQKRLRPIASGQLPVPVAATAAILFVVITLPLAW
                     WLSPGFGIIAAAYFLVNLAYSQWLKHIPLIDVLVIAAGFVLRVAAGVTLITVERFSPW
                     LYVVTTLLALYLGFGKRRAELTLLLHGANAHRRVLDGYTIPLLDQLITIVSGTTIVAY
                     SLYTFSAPNLPENHTMMLTIPFVLYGIFRYLYLIQVKEAGGAPEELLLSDRPLQATVI
                     LYGIAVMVIFALFD"
     gene            23385..>23653
                     /locus_tag="PKL11_01695"
     CDS             23385..>23653
                     /locus_tag="PKL11_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNZ12339.1"
                     /translation="MEKQRLDLLLVERGLAESRSLAQRLVMAGQVRVNGQVELKSAAK
                     VALGADLVVDHGPPFVSRGGEKLAAALSAFGLTDLQHRVCVDVGAS"
     assembly_gap    23654..23753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23754..24347
                     /locus_tag="PKL11_01700"
     CDS             <23754..24347
                     /locus_tag="PKL11_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNZ12340.1"
                     /translation="VCVDVGASTGGFTDCLLQHGAARVYSVDVGYGELHWKLRNDPRV
                     VVMERTNARYVTGFPEPVNMIVIDASFISLRTILPAVKSWVSPENPCIIVALIKPQFE
                     AGREDAARGGGVIRDPAVHRRVLEQVIESAQELGFVLHGLIRSPLLGPKGNIEFLVHL
                     SYPGTGGVDSKLLVDSALAENKTGIQSSIEDSAEVNP"
     gene            24344..24994
                     /locus_tag="PKL11_01705"
     CDS             24344..24994
                     /locus_tag="PKL11_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNZ12341.1"
                     /translation="MKDAYIQKLLGEQEVVLLATRQHWFMLFGSIILEIVLILVIIGA
                     AVLINVLWASLPFLGSLIIIVLPVILVAILLVTLVRDILVWSNRKYIVTNRRVIQVNG
                     IINKNVIDSSLEKVNDVKMIQSFWGRLFGFGNIEILTASEMGVNVFRRIGNPIRFKIA
                     MLNAKEQLGHDENGISHAPATRSVPQLIFELDELRKRGLITEEEFQQKKKDLLSQM"
     gene            25120..26922
                     /gene="lepA"
                     /locus_tag="PKL11_01710"
     CDS             25120..26922
                     /gene="lepA"
                     /locus_tag="PKL11_01710"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNZ12342.1"
                     /translation="MTTAHIRNFCIIAHIDHGKSTLADRLLQITGTISDREMVAQVLD
                     SMDLEREKGVTIKASAVRMNYTAQDGQTYELNLIDTPGHVDFNYEVSRALEACEGALL
                     IVDATQGIEAQTLANLYLALEADLKIIPVLNKIDLPSSHADEVAKDLEHLLGIPADSI
                     LQVSAKDGTNVPAVLEAIVQQVPPPAGQPEGVLKALIFDSHYDSYKGVVAYVRVVEGQ
                     MKSTDMLHLMATNIDMRPVELGVFAPDMRPVPSLQAGDVGYVATGLKTVSECHVGDTI
                     TAATQPAAEPLPGYRRPKPMVFAGIYPVEGEDYADLKEALEKLQLNDAALVYEPETSQ
                     ALNFGFRCGFLGLFHMEIIQERLEREYDLDIVVTAPSVEYEVVLVTGEVLLIDSPVQL
                     PDESTIAEIREPWMRLEIITPTEFYGTIMELVIKRRGAFKSQDYPAPNRVQLVFEIPL
                     AELIVDFFDDLKSRTRGYASMDYAVDGYRPENLVKLEVMVGGEPVDALAAIVHKEHAY
                     HKGQRLVTKLKELIPRQLFDVAVQASAGGRIISRANVKALRKDVLAKCYGGDISRKRK
                     LLEKQKKGKRRMKMVGSVEIPQEAFMAVLRLEDE"
     gene            26919..27944
                     /locus_tag="PKL11_01715"
     CDS             26919..27944
                     /locus_tag="PKL11_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ12343.1"
                     /translation="MTQSPPTSPWRRVGRWLPGVLISLVAIIALSQFVKDGASLRAAF
                     AQINLLYLALVVLGTLAFLFVRAQAWRILLGGKPTIIQTFFAINEGYLLNNVLPFRAG
                     EFARAIFLGKSTGLGTFHILSTIVLERIFDVAFAAGLLLATLPMAFAMEQARQVAMIA
                     LALVVVGLVVLFWSYRNRARLEVWLDKFAGRWSFARRFILPQFSRLLQGFEVLSRPSQ
                     FLGALFWIAISWGIAVFNYWVYMLSFAPHAPFWWGAFADSILGLSIAIPSAPAALGVF
                     EAALVGALTILGLSEGPALAYAISLHAVQFVTTGILGVWGLLRERQSIQGVLDSLERK
                     PTPQQDA"
     gene            27945..>28414
                     /locus_tag="PKL11_01720"
     CDS             27945..>28414
                     /locus_tag="PKL11_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ12344.1"
                     /translation="MNILIALTYYRPHYSGLTIYTERLARALAQRGHRVMVLTSRFDP
                     RLPAREVRDGVEIYRPRVLMRISKGVIMPAMPFWAIRLIQQADVVNLHVPQLDAAPIS
                     LLARLLRRPVVLTYHCDLHLPKGFVHKVANWVSNLANHITARAANRLVTNTLDYA"
     assembly_gap    28415..28514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28515..28971
                     /locus_tag="PKL11_01725"
     CDS             <28515..28971
                     /locus_tag="PKL11_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ12345.1"
                     /translation="ARVLFLGQYQNVLGEEEYARRLAPLIDQIGSAWKFLGVVPSTAL
                     AAFFRLCDLTVLPSINGTESFGIVQVESMMSGTPVVATDLPGVRQPVRMTGMGRIVPP
                     RNALALGEAIIDVLDHPDKYQGDPAQIARMFAPASIAEQYEKVFGAVLS"
     gene            28968..29765
                     /locus_tag="PKL11_01730"
     CDS             28968..29765
                     /locus_tag="PKL11_01730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ12346.1"
                     /translation="MTSKDHLWLQLKSMPYFRGLLRAVEARFYDDITLTGPVLDLGCG
                     DGHFASVVFDHPLDVGLDPWTAPLVEAAGRRVYNLPIQAAGAQMPFPDAYFNSAISNS
                     VLEHIPNLDPVLAETARVLKPGALFVFCVPNHHFDPNLSVAKLLDRLGLRKLAATYRR
                     FFDRIARHHNLDAPETWIPRLEKAGFTVERWWHYFSPAALAVLEWGHAFSLPALVTRK
                     LTSRWILAPYRWNLFITESITRPYYNEPSPQPEGVCTFYIARRRSQE"
     gene            29767..30573
                     /locus_tag="PKL11_01735"
     CDS             29767..30573
                     /locus_tag="PKL11_01735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNZ12347.1"
                     /translation="MANTLRKYQYYLSSIPRLLTGFVEWPKILGVFLRLYRTTPFEVQ
                     LRHSGVRFSARSPMDLWSIKEAFIDRFYERYGFPIEKRWTVVDIGAGIGEYTLLAATT
                     DTSNRVIAFEPFAESYKLLRQNLRLNGAINVSAVPIAISSRTGQLSLDLSAGEPLKIE
                     SHLAEAESPTGTAGLVECLSLADAFERYEIEHCDLLKLDCEGAEYDILFYASAETLSR
                     VKRIVMEYHDGKTAYSHKDMEHHLRDHGYRVESVVNVVHDDIGYLSAIRP"
     assembly_gap    30711..30810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30811..32493
                     /locus_tag="PKL11_01740"
     CDS             <30811..32493
                     /locus_tag="PKL11_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ12348.1"
                     /translation="NLLLWLAAVGSMVAALWAPRIGFADRLASLWGWLTRRQWQIRIT
                     PWMLLCVVVFAAAIFFRFYRLDSVPPEMVSDHAEKLLDVSDVLSGNTSIFFTRNTGRE
                     PFQMYLTALVSLVFGTGASFLSLKLGTVLCGVLTLPYLYALGKELGSRRIGLIALAFA
                     GIAYWPNLISRIGLRFTLYPFFFAPTLYYFLRGMRTSSRNDFILAGLFLGLGLHGYSP
                     FRIVPLLLVFAIGLFYLHRVSKYRRSESIAGLAIIILVSVVVFLPLLRFLLENPAIFS
                     YRMLTRLTGMESPLPGPIWQLFFTNLWRALTMFSWNNGEVWVISVMGRPVLDWVAGGL
                     FNLGVVVLLVRYIQRRHWLDLFLLLSIPILMLPSILSFAFPAENPCLNRTAGAIIPVF
                     LIVGFALDGVMKSLESHLARLGSRLGWLLFAGLFLISAAQNYQLVFNQYRSFYEQASL
                     NTSEMGQVMGAYAEWSGSPETVHLVGYPYWVDSRLVAINAGYPTLDPGIMPENLPQVA
                     TDNRAQLFILNPYDTDGVAALNLLYPGGTLRNYDSKFDGKDFLLFYAPAKMP"
     gene            32518..>37754
                     /locus_tag="PKL11_01745"
     CDS             32518..>37754
                     /locus_tag="PKL11_01745"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNZ12349.1"
                     /translation="MDDPLTPVQVTENSTPSPSTEKPPRRKFSWGWAWDLLLVVILLV
                     GGYFRTIGINWDSNQHLHPDERFLTMVETSIQPVASLADYFNTATSTLNPNNVGHGYF
                     VYGTLPIFLVRYAAEALDQTGYDQVYLVGRAFSAFIDLLTVFLVFVIALRLYRNTRLG
                     LLAAAFSAFAVLQIQLSHFFTVDTFANFFTYLAFYFAVRVMTADTPGTPVSSPTETAE
                     ADMPPARPSPWAWMQLSWRGWGNYILFGLALGMAVASKISAAPLALLLPLAAVLFISR
                     IPKPQQRDQIIIAFRHLVLAALMAILVFRIFQPYAFDGLGLNPKWVANLTELQRQSTG
                     DVDFPPALQWARRPITFGWENLTIWGLGLPLGLLAWAAFIWMGWRIFRGEWRNHLLLW
                     GWTGLYFLWQGTAFVSSMRYLLPIYPSLAIIAAWGIFALWEAGKKKPLPRWNWQKILS
                     VAAGAIVLVSTFAWAFAFTRIYTRPVSRLEASYWIYQNVPGAINLRMTTADGDYQQPL
                     GSLGALTLMSDHPLTLTFDAPAAVTLQDITITRLSDAQWSADQRTLSFSIYAANSPDS
                     TVLASGMLASSYAVEQSTTIPLSIPLTLEPATEYVIQFSVMEPGAMMRLAGYLDLGLQ
                     DGERLYRHRAAEFVQLITTEQSFQVPSFRILEAGTLNGIWLENVADWESQPDPKTLRV
                     SLALPGQESQPLAVAQIQDTFLRQNDFRGESYWLAFDPPVQIIQDGVYTLTFELPEGT
                     GALALTNSKVAIESSWDDAVPLSVGQGGPYDVNWGPYRTDLNFEMYWDDSADKLERFI
                     TILNNTDYIFMSSNRQWGTIPRVPERYPLSAYYYRHLLGCPEDKDIVWCYSAAEPGMF
                     QGDLGFELIYVGASYPNLGSLEFNTQFAEEAFSVYDHPKVMVFKKTADYQPTATQALL
                     ESVDLSNIVRLTPRQAGDYKGNLMLPANRLVKQQSGGTWSELFNTDALHNKYPALALI
                     LWYVVITLLGWLIYPFVRLAMGGLTDKGYPFARLIGLLLLAWVTWLAGSAGIDFSRLT
                     ILIIFVLLSLGSLALGWLQRDALREEWRTRKRYFISVELIGLAFFVLFLLVRIGNPDL
                     WHAFKGGEKPMDFSYFNAVLKSTTFPPYDPWFSGGYLNYYYYGFVIIGVPVKLLGIVP
                     AIAYNLILPTWFSLMALGAFSVGWNLANSVKKPAETDQQPQPVAALDILLSEPADTES
                     PIAPAIAPSEYIDSPAPASPVAESTQVPVAPTKKHRPATWNLYMAGITAAVAILILGN
                     LGTIRQIWHSIQRLAAPGAVIEDATFVQRLTWTFDGLAKYMDGEKLRVGTGDWYWVPS
                     RAIPGEAITEFPLFTFLYADPHAHLIALPITVLAIGWTLAVLLGRWRWADRRRALAFG
                     ASMLLGGVVLGALRPTNTWDMYTFLPLGAIVLGYTAWQYADWPIRWLSDWPQKFQRFV
                     VALASMALLVILALLFYAPFTHWFGTAYNSFALWKYDHTPFWSYITHWGVFLFVLIGW
                     MYWETRDWLAKTPVSALKKLEPHLIWLIPVGLIILALIILLMSGITTAGLSDEVLVQL
                     KTPGVAVAWLVIPMILWAGILILRPAQPDGKRMVLFMIGTALTLTLVVELLVLVGDIG
                     RMNTVFKLYLQAWTLLSLSAAAALYWLWPEINLNWTPRWRSTWQVVFALLIGSALLFP
                     LVAGADKIRDRMSNQAPHTLDGMAYMEYGSFYENGVNIEFDEDYRAIRWMQENVTGSP
                     VIVEGRVGEYHWGNRFTVYT"
     assembly_gap    37755..37854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37855..38204
                     /locus_tag="PKL11_01750"
     CDS             <37855..38204
                     /locus_tag="PKL11_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12350.1"
                     /translation="VEGRVGEYHWGNRFTVYTGLPNVLGWNWHQSQQRAGATQPWVGQ
                     RLNDVMLFYTTVERSETENFLERYNVSYIIVGQLERAAHPGPGLDKFVQWEGDLWQEV
                     YRDGQTVIYEVIR"
     gene            38201..38554
                     /locus_tag="PKL11_01755"
     CDS             38201..38554
                     /locus_tag="PKL11_01755"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HNZ12351.1"
                     /translation="MTPSELIYHLTIQSDWQTALQIGEYRGNTLETEHFIHCSTRTQV
                     LEVANRIFKSRLDLLLLELDPALVVPEIKWEISENDDTYPHIYGPLNLNAVRHVWAMP
                     PESDGTFVWPVGAGE"
     gene            38560..>40513
                     /locus_tag="PKL11_01760"
     CDS             38560..>40513
                     /locus_tag="PKL11_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNZ12352.1"
                     /translation="MLAFLSWYVLITVVGLLAFPIAYRIFTRLPDRGYALARPFGLLL
                     WGFIFWLLASLHILQNDLGGVLLALLLVLGASALVIRKEWQDFIGWFKPHLKIWLTIE
                     CVFLVFFAAWAAVRAFNPAIVGTEKPMEMAFINAILRSPTFPPNDPWLSGYSISYYYF
                     GYVMVAMLTRLTAVEPGVAFNLASALWFALTATAAYGVLFALINRWLHERTPLVETLK
                     TTARGLGMALFGPLFLLLVGNLEGILENLYAVGAFWSPAADGTYQSRFWAWLDIAELK
                     AAPTHLPFDWSAWNFLKDGLPHRQAGWLIWRGSRVLTDYNLSGGAIEIIDEFPFFSYL
                     LADLHPHVLAMPFVLLCIGLALQVYLAHGRPFEGKSIFDWLRLPEFWLNAVIFGSLAF
                     LNTWDFPIYVALFSAAFILSRTQSFGWATRRLWEFIGVAAVLGIAGVVLYLPFYLGFA
                     SQAGGIMPSGIFVTRGAHFWVMFGVLLVPVLIWLFWSWSHNGSRPALKAGGKFAVWVI
                     GGLALASLSLLVLAPVLESIGRSILSTPSGAASRLANSMVFWASMINSLFGASDGGAL
                     LGAALQRRITYVGTWLPLTAIVVLAWGLMSALKRRPASEESTSQHIAPIQAFVALLAL
                     LGAGLALFPEFFFLRDNFGNRMNTIFK"
BASE COUNT         7941 a        11587 c        10336 g        10149 t
ORIGIN      
        1 ctcagcgtct ttgaaaagaa caggtaattc atgaccacac ccgagaccga aataaccccg
       61 cgcccccagc gaaaaatctc cattttgcgc ctgtttggca cccttctttc cattggcctg
      121 ctcatctggc tgattgagcg tgaggggtgg gatgccatca agtctgccgt tacagaaatc
      181 tcctggtgga atctggtgct ggtcctgttt ctcacttttg tttcccgctt tgcggtcacc
      241 ggtcgctggc atgtgctgct gcgctctggc ggcgtaccca ttcggctgcg agaatcaatc
      301 cgcttggtgt tcgctggtct attcgcctcc aatttcctgc ccaccactgt cggtggtgac
      361 ctgattcgat ttgccggtgc tgttcgcctg cactacgacg ccgccatctg cgctgcctcg
      421 ctcgtgatgg atcgcctggt tgggatggtg ggcatgttga cagtctttcc aattggcgtg
      481 gcccgcctct tgagtatgcc tttgccgccg ttaccagccg gtgggtattc aggcggcttg
      541 gcaatggccg tgtctttgac cggaatttgg aaatgggttt ggagcaaagt tcaaaaactc
      601 ttccgctcca caatgcacgc tttgcggctt tgggccaccc atccccgcga cctcttcagc
      661 gcctacttgt ttacgctgat acatatggcc gctctgtttg ccacagtttg gctcatcctg
      721 cacgatctgg gtcagcccat caacttctgg caggttgcgg gcttgtggag catcagctat
      781 ctggtcacct tactgccgat ttcaattaat gggctggggg ttcaagaatt gtccctgacc
      841 tttttattca ccaactatgg cgggctctcg gttgagagtg ttgcggtgat ggccatcctg
      901 attcggctgc tgcagatggc tgccagcctg cccggagcca tttttgtccc tgcactgatc
      961 cagcccgaag ccccgcctgc tgccccggag actcccgcat gaaattaccc cgcccaatct
     1021 ggattgcacg gctgaacctg ctggtttccg tcatcctttc aatcctgact ttgctcgaac
     1081 tcgaaaaatt aggatatggc tctggcgagt tattgggcca ctacgccctg ccgcggctga
     1141 tcatgctggc gttctttgcc acagttgggc tggtttgcct gattctgctg gcgctcacct
     1201 ggcgccttcg catggcagat actctgctgc gcattttaca gaagctgccc cgcgccaaag
     1261 ctctcaatat tgtggcgttg gttctcggtg cgctggtttt gggctggctc gcttttcgct
     1321 atgataacgg caagttcagc acccccgtga tccgcctttg gctgatctgg ttcacagcca
     1381 tttttggcgc cttttttatc cagcgtctta agccgatgcc ctacgccctc gctctggcct
     1441 cggctttact gatagctggg gcctggttcc aaattctcac tttcctcccc ggtctgacgg
     1501 atcatccgct gtcgttgggc tggtcagagg gcagccgctt ttattatggc tcgctgttct
     1561 ttgccagaca gatctatggg gaagttttac cgattcccgt cctgcatcct gcccgttatc
     1621 tgatgcagtc cctccccttt gcttttccca gccttcccat ccttgctcac cgcatctggc
     1681 aggtgatttt atgggtggtt tgtaacgccg ctgccgcctg gctgcttgtg cgccgcctgc
     1741 gcctgaatag tcgctggttg gcctggatgg ctgcctgttt tgccttcctc ttcttttttc
     1801 aaggccctgt ctattatcat ttgattttat gcacgttgcc cgtcctggcc tggtttgaca
     1861 gccgccgtcc ctggcgaacg ctggtggtgg tgctgttatc ctccctttgg gcagggctct
     1921 gccgcgtgaa ctggtatgtc gtgccgggcg ttctggccgc caccctctac ctgctggagc
     1981 agcctcagcc gcgcttttcc tggcgctatt gggtttggcc tgcgctccac gccattctgg
     2041 gcaccgctgc cgccttactc ttcagcacct tttacaaatc catatcgggc aactcgcccg
     2101 aggtctttgg ctcggccttt gactcgccct tactctggaa tcgcctcttc ccaaatacaa
     2161 cctacccttt ggggatgctt ctgggaacag caattgctgt attgccgttc ctgttgatca
     2221 tactctggtc tttttgggcc aaccgccgcg cctggcatcc cttgcgcctg ttgggattgg
     2281 gcgccattct atttgtcttt ctggcgggcg gcgaggtggt cagtcttaaa attggcggcg
     2341 gcagcaatct tcacaacctg gacgcttttt tggtgtttca ggtggtcatt ggcctctatc
     2401 tgatatttaa ccgctttgta cccgatgcgc acccaattcc agcggccaag ctgcagcctg
     2461 cctggtggct ggctggtttg acgatagtca tccccttctt ccagatgatt ggcggcgggc
     2521 agcctctcca cctgccagac cggacgaaag tgtccgatga tctatctcaa ttacaggtta
     2581 tgctaaatca ggccgatgga ccggttctgt tcttgactga acgccacctg ctgagcttca
     2641 aaaccgtcac tgcagcagat tttgagcctg aatatgagaa agtatttttg atggaaatgg
     2701 tcatgtcggg caatcagacc tatctcgacc acttctattc tgatttacag cagcaccgtt
     2761 tcgccctgat tgtctccgaa ccgatgaaca tgaacatgca ggacgagcgc tttgcttttg
     2821 ctgaagaaaa taattactgg gttcagcggg tcgagcagcc tgtatctcag tattaccgca
     2881 ttttggatga atttaacgat tctggtttcc acgtctgggt ccccgtggag tagttatgct
     2941 ggatctgctc gttctgggta tttgggcctt attctggggg ctgggcggca tctggttggc
     3001 tgccgctgcc tttcgactga cgcgcagtga acagttgctg gtcggtctgg tcctggggct
     3061 tacgcttgag accttgctgg caaatattct tgggcgcttc atcttgcctg cgccagcttt
     3121 tttgatagct gccggcttaa cctttctgct aggcgccatt tttattgttt tcaaaaacgg
     3181 ctggcgcgct ttgtaccatt tcccattgga ctggcgtgcg gctcttgcgc tcctcgtcat
     3241 caccggcttg tttactcaaa ttggccgtgg cctggcaatt tttgatgatt atgctcacct
     3301 tcccaccacc tctttactgg ctgccggcag tctgccgcta aagttcgccc tgaaccccga
     3361 tgccccctat acctatcatt acttcttgat gctatttgcc gcccagatca cccgtctggg
     3421 tgatctggct gtctggtttg ccctggacct cagtcgggca tttgcttttg gcctcacaat
     3481 tgttttggca ttcatctggt cgcgccgcat aacccgtggc attcctgccg gcattcttgg
     3541 cgctctgaca gtcctttttg ccggaggctc ccgctggctg gcgttgctgt tccccggcgg
     3601 ttggctggaa aacgcttccg ctcagatgac gatggttggc agtggcagtg ccagtggctc
     3661 cacttttgct actgccttga ccggcccttg ggcaattgaa ggcggcgcct cgctggattt
     3721 tcctttcgct tttgtgaacg gtgttttgca gcccggtgtt ctgtccatgc tgggtcccaa
     3781 tagcaccttt ttgctggcgc tcacctttac actgctgtta acctacaatc gttggcggtc
     3841 ttgggttgga gcgattccag tagttatctg ggtggcagct tttagcctgt taaccgagtc
     3901 cggcattctt ttgggtcttg cgggctgggg cctggtgctg gtcgcggtat taattaaaca
     3961 acgcaattgg cgcatccccg ccgccctgtt aacctggctg ttggcctggc tcactggaac
     4021 actgctgggt gccttccagg gcggcgccct ggcacagatt atcctcgatg cttttggcaa
     4081 atcatctgcc gcggcctatc agaccgtggg tttccagttt gcctggcccc ccattttagt
     4141 ttccacgcat cttggcgcgc ttgacttatc ccagcccgcc acgttgcttc tggcgatcat
     4201 cgaagccggt ccgctaattt tcatcctccc cttgaccatg ttctggggtt ggaaggctct
     4261 gcgcgcgggc cgctggtatg aagctgcctt gatcacttca gggttattgt cgttaggcgt
     4321 cattttcgtc cagttcgctg gctcaaccgg catccgcaac actgcccggt tgtatgagtt
     4381 tatgacgctg agcctggtct atgccgtccc cttgggctgg ctgtgggcgc agcggcaggg
     4441 ttggctgcgc aaaaccatag ccttgtctgc gctctgtatc gcttctctgg gtgggctggt
     4501 cgtctttggc gtggaattga ccgccatgcc tagcccgaca caatcatatt atctcaatga
     4561 cctcgatgcc gtgatgatgt cacggtactg ggataagtta gagcccgacg ccctggtttt
     4621 tgatccgctg cccaaccggt ccccggtttt gttcgcccgt tacaccaacg ccgcccaaac
     4681 ctggtacgcc tacaaacccg aatggctggc gctgaagact aacccgaacc cggtcgcttt
     4741 acatgctgcc ggttatgact acctctatct ggattcgacc tattgggaag agctcagccc
     4801 gactgttcag gtcagtattt ctgatggctg cgctcaacag attgatcaaa ttgaagactg
     4861 gcgccacgat acccgttggc tgtacgacct gcgagcctgc ggcgaataac caaaccgctt
     4921 acccaacaga attgtaagca ctggtcataa gggatggggt ataatccttt tttgcacagg
     4981 tgccaaaatg aacattgcgg aatttcgtat agcaaaagaa taccggaccg atcagcgcgg
     5041 accggtgcgt tgggtttttt ctcatgccat ccggcagtgg ccattattgc tgatggcatt
     5101 atttggtgca gtaggcaatg ccgccttagc ggcagtcctg cctattttga tgggtgaggc
     5161 ctttaatctg gtgctatctg gttcgccaga cctcactcga ctgggtcaga ttgcgatcac
     5221 gctggctatt tctcagctca ttcgtggcgt tatccagttc agccgtaatt tcggttttga
     5281 atggatcgct cagcgcgttg agcgggatgt tcgcgacgaa ctctacatca atctgctcgg
     5341 caagagcatg accttccaca acttgcagtc ggtgggtgat accatgtctc gcgccaccaa
     5401 cgatgttcga gaagtcaatt atcttttcag ccctggcatg gcccaggtgc tcggctcgtt
     5461 gaacttcttg atcatgccgc tgatctttgc accgcgctat caccccacgc tgattctcac
     5521 gccgttgatt tttgtgattc tgtattactt tgccctgcgc aattacctgg ggcagctccg
     5581 tcccgttact gaccgggttc gggcgacctt tggacaatta aacacgcgtc tctctgaggc
     5641 cttggatggt attgagacgg tcaagggctc agctcaggaa gatgccgaag tggcccggtt
     5701 tagcgccaat gcttccagct accgcagtgc cgtagtgcgt cagggtgatg ttgaggcgcg
     5761 ctttattccc ctcttgctgc tgaccctggc tctgggtttt ggcctgctgc acgctctgat
     5821 cctgtttcgc cagggcattt tggatgttgg tcaggtcgtg gcatattttg gtctgctgtt
     5881 gatgctcgat tttccgacct ttgcctccat gtgggcgtat tcgcgcattt ccctgggtct
     5941 ggcaggggct cgccgcattt tagaccttat gcatcgcgaa acctatctgg acgagaacgt
     6001 ctccggtcac caggcgcaaa tgcgcggcga agttgagttt gaggaggtct gttttgccta
     6061 cccccaacaa aacaacgccg aaaccgatgc gtgtgatgat ggcattctgc agcacatttc
     6121 ttttcgtgtc aaaccagggc agaccgtcgc tattgtgggc caaacaggca ctggcaagac
     6181 aaccctggtg cgcctgatca atcgcatcta cgattcaacc tcgggtacgg tgcgcgtgga
     6241 tggtttggat gtgcgagatt ggaatctggc agtactccgg cagcaaattt ccatcatcga
     6301 gcaggatgta tttctttttt cccgcaccat tgccgagaat attgcttttg gcaaacccga
     6361 tgctacccag gagcaaattg aagccgctgc tcgcgccgct caggcccatg aatttatcac
     6421 gggctttagc gaaggatacc aaaccgccgt gggggagcgc ggcgtaacat tatccggtgg
     6481 tcagcgtcag cgcattgctc tggcgcgcgc gttcctgacc gacccgcgta tcctgattct
     6541 ggatgactcc accagtgcca ttgacagtgc cactgaagat aaaatccagc gcgcaattta
     6601 caccgccgcc cgcggccgca ccaccttcat catcacccat cgcctttcac aaatccggtg
     6661 ggccgatttg atcctggtcc tgcgccgtgg ccaattggtt gccacaggca gccacgatga
     6721 actcatgaaa acttcggcag cctaccgccg gatttttagc gaatagagga atcagatggg
     6781 attctggtct ggacttaacg ccgaacatta cgatcgcaaa tattctgatc gcgagctctt
     6841 ccgccgcatt ctggcgcaat tccgcccaca gcgcgctcgc atgggctgga ttgtggttac
     6901 ggtcttgatc ctggccgcgg ccggggccgc aaatcccctg gttgtctccc gtggcgttga
     6961 tttattgaaa gatcagatct ctggctggac aatggctgcc atctctggcg cagtcatatt
     7021 gcttggcggt atcagctggc tggcaaatta cgcccagcgc cgcctggccg tccgtgcggt
     7081 ttccgatgtc gtcctgtctc tggcgaccaa cgcctttgac gccgccgccc gccatgacat
     7141 gtccttccac gatgaggttt cgtcgggcaa aattgtctcg cgcatcacct ccgatactca
     7201 ggattttggg cagctggtca tgctgaccac cgatgtaatc gcccaactct ttgaggcgct
     7261 ggtgctgggc gtcattttgc tcgtgattga atggcggctt tccctctata tctttgcgct
     7321 catcccggtt gtttttgtct ttgcaattgc ctatcgccgg ctggcccgtc gggtaacccg
     7381 tgaagggatg cgcgctatgg cgaatgtcaa ttccacaatt aaagaaacga tcagcggcat
     7441 ggctgtggcc aaaaattttc ggcaggaagc cagtgtttat cgcgattttt ccacggccaa
     7501 cctcgcctca tatcaggtga acttccgccg tggcttggcg ctctcatttg tctttcccac
     7561 cctcaatacc cttggcggca taatgtctgc ccttctgatc tatgtgggcg gcttgagcgt
     7621 cgccgatggt ctggtgaccg ctggagcctg gtacttgttc ctctctagcc tcgatcgttt
     7681 cctcttcccg gtgatgaata tctcatcctt ctggacacaa atccagaatg gcctctctgc
     7741 ctcggaacgc gtctttggtt tgattgatgc ttctcctgcg gtagttcaga ccgattcgca
     7801 aattgccccg cacctgaagg gcgaaattat ctttgaccac gtatccttcc gttacaaaga
     7861 aaccgaaccc gtcctggaaa attttaattt acgcattgcc cccggtgaga acctggcttt
     7921 ggttggtcac accggcgctg gtaaatcatc ggtcgctaag ctcactgccc gcctctatga
     7981 gtttcagcag ggccgcatcc tgattgatgg cctggatatc cgcagctttg acctgaactc
     8041 ctatcgccgc cagttgggca tcgtgtctca gattccattc ctgttttctg gcactgtggc
     8101 cgataatatc cgttatgcca acccaaccat ctcggactcc gtcattgaag acctcgcccg
     8161 ccagattggt gatggcgagt ggctggaggc cctgccagac ggcctgcaaa ccgatgtcgg
     8221 cgagcgcggc gcccgcttat caatggggca gcgccagctc gtttccctca tgcgtgtttt
     8281 ggcccagcgc ccagccatct ttattctgga tgaggccacc gccagcattg accccttcac
     8341 cgaatggcag attcaacagg ccctcaagct gatccttgcc caatccacct ccatcttgat
     8401 tgcccatcgc ctctccaccg tgcgctctgc ggaccggatt cttgtgatgg agcaggggca
     8461 gatcatcgaa gaaggcagcc acaatagttt actgcagcgg ggtggacact acgccacact
     8521 ctataacacc tatttccgac atcagagcct ggcctatgtt gaacaggcgc aagagatgcg
     8581 cagcgccctg accacggaac caacaccatg aacgctgcct attacaagaa cctgggcgat
     8641 cagtacgcag ccgccaataa gctggaagag gctttgcgcg cctatgaacg cgccctggag
     8701 cttgacgcca atgaccccga cctgtatttt gcccgtgccg aggtctaccg ccgcctgggg
     8761 cggcttgacc cttcgctcga agattacaac cagggcatag aactttcccc cctacgtccc
     8821 gagggattta tcggccgcgg caacgtctat ctggcttggc gctattatga tcgtgccatt
     8881 caagaatttg ggcgtgccat cgctcttctg cccaatgggg ctgatgggta ttattatcgt
     8941 ggtttggcct atttgcagct tcaaaaattc cagtttgccc tgacggacct caatcgtgcc
     9001 ttacagctta agccagatta tctggaagct caggttcagc gcggcctcac ttaccatgcg
     9061 attggcgatt ttcagaacgc attgcgagat ttcaccaccg tcctcgcgtt ggatgcgcgt
     9121 tacgccctgg ctcactatta tttggctcaa acctataaaa aactgggtcg tgatgaactg
     9181 gctgtggggt ctctggtaac cttccaatct ctggtgcaga gctcaacctg gcagcaaaaa
     9241 gcccgccagg aactggaaga gttgcgcaaa catgctggta caccccgctc tacccagcca
     9301 ttgcgcgctg tccaactcaa cccggccgat cgtgccgaag tcttgcggct gctgcggatg
     9361 ggggataagg ttgccgcgct taaacgcgtt gcggaggcca gcggcgcggg gctgcgagcc
     9421 tccaaagaat atgtcgatga attaatccgc acagaactgc gcagttgatt acattcctgc
     9481 catatacgcc agtcccatag cgccaggccc cgtatgcgtt cccagcaccg ggctgatatc
     9541 agcaataaat gtctcactga cgtctgtcag tccaaacctt tcccgggcac gtgccagtag
     9601 ttcccgcgcc tcagcaggtg catgagcatg cagtgcagcc aggcgaatgg gcgtccgtgt
     9661 gccgatgcgc tcttctgtca aagcaatcaa ccgatccacc gccttgccca tagttcgaac
     9721 ccgctcaatg gcctcaatcc ttccctcgcg cagctccagc agcggcttca agttgagtgc
     9781 cgtgcctaaa aaggcggctg ctccgccaat ccgtccgccg cggtgcaaga attccagcgt
     9841 attgaccaca aagcaaaccc ccgattgctc tgttgcccgc tcgaccagca ccctgcactc
     9901 ttgcagcgtg gcattttgag ccgctgcgcg cgccgccagc atcaccggaa agcccatcgc
     9961 catagatgtc aattgagaat tgaccacctc aatcggcgca ttggtaaaat gcgcctttgc
    10021 ctgcagcgcc gaatcaaccg taccagataa tgccgctgaa atatgcacac taagaataga
    10081 ataaccctca tccaaaagct tttgataaac cttgataaaa gccgccggag atggctgtga
    10141 tgtggtaggc ataaccttgg tatgcttcaa tcgttgataa aaatccgccg gctggatatc
    10201 caagccatcc aaaaatgtct catcccccca gatgacctgc agcggcacaa cctgtaccgg
    10261 ataaccgcgc atcagctcag ccggtattgt cgctgtgctg tcagttacaa tggcaacttt
    10321 agacataatt tttctccttt gcggtaatta aacgccatgc atataaaccc acatcacaat
    10381 cattggttac tctttcaaat caagccttga ccattcgcct cgaacaggct aaaatccagt
    10441 gtactctgac cacccaattg ggcggttaat ttaatgatta agacctgacc agtgtttgag
    10501 aacctgaccc aacgcctcaa ccagatattc cagaatcttc ggcgccacgg caagctgtcc
    10561 gccgaagatg ttgacgcggc tatgcgcgaa gtccgcatgg cgttgctgga agcggatgtc
    10621 cattacagcg ttgttaagga ctttgtagcc cgggtgcgtg agcgcgccgt cggacatgaa
    10681 gtctctaaag cccttaatcc tgctcagcag gtgatcaaaa ttgttcatga agaactgatt
    10741 gccaccctgg gcgaacctgc ccgcctgaac ctgacgggcg ccaaaccccg cgtcatcatg
    10801 ctggttggtt tgcagggctc gggtaagacc acccactccg ctaaattggc tcgccagctt
    10861 cgcgagcagg gcgagcgtgt catgttggtc gccgccgatc cttaccgccc ggcggctatt
    10921 cgccagcttc aaaccctggg cgaacgtatc ggcgtgccgg tctttaccga aaacggcgtc
    10981 aaagtccccg atttggctgc ccacgccctg gatcaagccc aaaagggcgg ctactcggtg
    11041 ctgctgatag ataccgcagg tcgctcacag cttgaccagg atttgatgga tgaactgcgt
    11101 gccatccata agcgcgtcaa tgcctctgaa atcctgctgg ttgtcgacgc catgatcggt
    11161 caggaagcct tgcacgttgc cgaaggcttc cgcgatacgg ttgccatcac gggcctcatc
    11221 ctgaccaaga tggacggcga tgctcgcggt ggggccgcca tttctatccg tggtgttacc
    11281 ggcgtaccga tcaaattcct cggcaccggc gaaggcctcg aagccctgga gacctacgac
    11341 tcgggccgcc tggcctctcg catcctgggc atgggcgatg tcattggttt gatcgaacgc
    11401 gccgaagcag ccatggatgc cgaatcagcc caggatcagg ccgagcgcat gctctccggc
    11461 cagttcaccc tggaagactt tatcacccag cttcggcaaa tccgcaaaat gggcccaatt
    11521 caccagattt tggaaatgat cccgggctcc atggggcaga tggctcgcac cattgacccc
    11581 cgagatgccg agcaccagat gaaacaaacc gaagcaatta tcagctcaat gaccgtgcaa
    11641 gaacgccgtc atcctgatat acttaatgct agccgccgcc gccgcattgc ccgtggctgt
    11701 ggcatggacg tgcaggacgt gaaccgcttg atgaagcagt ttcgcgaagt gcagcgtatg
    11761 atgaaaaccc tgcaaaaaac aggcggacgc ggaatgccac gcctgtttgg ctaacccgca
    11821 ccggctgcgg cgcgctccct tcagcgcctc tcaaccgcag cgcaggtgac cgggtaccga
    11881 tttttatgag tcaatttcaa gaaagaggag tgtaagttca atggttcgca ttcgattacg
    11941 tcgggttggt ctgagcggtc aaattagtta ccgcatcgtg gttgccgata aggaagcccc
    12001 gcgcgatggt cgcttcctgg agatcattgg tcattacaat ccccgcaccg aaccgttcac
    12061 ctttgatgtg gacgaaggtc gggtctatga ttggatgaac aaaggcgccc aaccgtccga
    12121 atctgtccaa atgcttttcc gcagcgtcgg cctgctcgat cgctacgccc gcctcaaggc
    12181 tggtgaatcg ctggatgtgc tgctcgctga agccaaggcc tactatgagg cccgcaaagt
    12241 gaacaccagc actacggcgg ctcagaagaa ataaccggtc atgaaagcgc tgatcgaata
    12301 catcgcctgt tctctggtgg atgaccccaa acaggtttca gtccagcaag gtcgcgatgg
    12361 cgacaaagtg cggattgaac tacgggtcgc caaagaggat atggggcgcg tgatcggcaa
    12421 gagcggacgg gtggctaacg ccatgcgtgt tttgctccgc gtcgcggccg cacgtgaagg
    12481 gaagcaagtt accctcgacg tggcagaacc gcgatgaggc agccaaacca ttctgctgct
    12541 caatcaccgg accaggaaac aggctcgccg cagtccggcg ggcctgtatt tttggctgtc
    12601 ggccaactgc atcgtcccca tggcgtccgg ggtgaaattg aaatggaaat cctgaccgat
    12661 tttcccgaac gtctggttcc tggcaaagtg ctgtatgttg gcgaaaaaca cgcccgccac
    12721 cgcatcagca gcgtccgccc taagggaact ggccttctgg ttggcctgga gggctataca
    12781 gaccgggatc aggtggctgt ttttcgcaac ctgacgctct atgttcgctc tgatgagcta
    12841 ccttccctgc ctcccggcca atattaccat catcaattac tgggtcttca ggttgttgct
    12901 gaattcgggg aatccctggg ccgcctgact gaaatcatcg aaaccggcgc caacgaagtc
    12961 tatgttgtga ccgatgccgc tggacaggaa caactcctcc cagcaattga atcggtcatt
    13021 ctgggaattt ctcttgaacg gggggaaatc cgggttcgtc cccaggagtg ggagtaaatt
    13081 tttggacgag cgagcctatt ggctggggtt taatctcatt aagggcattg gggcagtccg
    13141 cctgcgcgct ctgctcgact ttttcggcga tctgcaaacc gcctggaacg cccctctgga
    13201 agccctcacc gcagccgggt tgagtgaaag acttgcgacc tctgctcttg ccgcccgcaa
    13261 agaagtggat ctggaagccc gctggaatga gctggaacgc aagcaaatca ccctgctgac
    13321 ctggaatgac cctggctacc cccgccgtct caaagagatt gaccaaccgc cgccagtcct
    13381 ctacctgcgc ggcgatctgc tgattgagga tgactgggca gtggcaattg ttggcacccg
    13441 ccgcgtaacc tcttacggac gccaggtagc cgaagaattg gcagcctatc tcgcccgcag
    13501 cggtatcgcc gtggtcagtg gcatggctcg cggtgtggat ggcgtagccc acgatactgc
    13561 tctgcgcgcc ggtggacgga cgctggcggt gcttggctgt ggggtagatg tcacctatcc
    13621 ccctgaacac ctgcgcctct ctgagcggat tatcgcccag ggagccctga tcagcgacta
    13681 cgctcccggc acccagcccg aaagcgccaa ttttccaccc cgcaaccgca tcattgccgg
    13741 cctctcactt gccacagttg ttattgaagc tggcgaaacc agcggcgccc tcatcaccgc
    13801 ctcttttgct gccgaccagg ggcgcgaggt cttagccctg cccggcaata tttatgcccc
    13861 tcaaagcaaa ggcaccaaca aactcattca gactggcgcc cgtccattgt tacgggttga
    13921 agacgtgatg gatgctcttt ccctggatcg tgtccaggag cagcgccagg cacgtcagat
    13981 tctcccggca gatgatctgg aagcccgcct tctggatatc ttgagcgctg agcctcagca
    14041 tatagatgaa attggcgccc agatagactt gtcaattgaa caggtttcag ctacactcac
    14101 cctgatggaa ttaaaaggaa tgatccgcca cgttggcgga atgaactacg tcaccacacg
    14161 tgagacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaaa aggaatgatc
    14281 cgccacgttg gcggaatgaa ctacgtcacc acacgtgaga ctcgggaagc ctatcgtgcc
    14341 tgaaacatcc aaccaatcaa cctatgccat catcatggct gggggcggtg gcacccggct
    14401 ctggccgctt tcccgccaat cacatcccaa gcaaacgctt caactcatca gtgatcgctc
    14461 gcttttccaa ttagcctgtg atcgtttgca aggctttctg cctcccgaac aaatcctggt
    14521 ggtcaccaca gccgatctgg ccgtgacttt gcaggcccag gcccccgata ttccggcaga
    14581 taattacttg ttggagccca tgcctcgagg cacggcttct gtggttggat tggccgctgc
    14641 ggtcttgcag gctcgctgcc ccagtgcccg catgtgtgtt ctgaccgccg atcattacat
    14701 tgcagacatt tcccgcttcc agtcggtaat gcaagccgcc ctcacggctg ccgatcaggg
    14761 ctggctggtc acactgggca ttccccccac attccctgcc acggggtacg gttacatcca
    14821 gcgtggccaa ccgttgggca atttcaacgg ccagctcatc catcgaattc agcgcttcaa
    14881 agaaaaaccc gatcttgaaa ctgcccaatc aatgtgcgct agcggcggct atgattggaa
    14941 ttccggcatg ttcttctggc gggtagatcg catcctggtc gagtttgcgc gctggatgcc
    15001 cgacttatct gccatcctgg cgcagttgac ggttgcctgg ggcagcccgg agtacgacca
    15061 gcgcttgcgt gagctttggc ctaccatcca accccaaaca attgattatg gcattatgga
    15121 acgcgccgaa cagaccgcag tagtcccggc gcagggcatc ggctggaatg atgttggcag
    15181 ttgggaatct ttgtttgaag ccctgcccgg agatgcgcac ggcaatatta tcttgagtac
    15241 agccagtcat cttgaacttg aagatcagaa ctcattaatt tacgccaata cccccgatcg
    15301 tttggtggtt acggttggac ttgaaaacat gatcgtcgta gatacgccgc aggctttact
    15361 ggtttgcccg cgacacgacg cccagaaagt tcggcaggtc attaccctgc tgaagcaaaa
    15421 cggaatggaa cgttatcttt aggttgagga gcaattttat ggaagcctat tgtgtcaaat
    15481 gcaagaccaa acgcgagatt gtagatccca ccccggcgtt taaccgcgtg ggttcgccgg
    15541 taacccgcgg cacttgcccg gaatgcggca ccaaactatt tcgcaccggc cgcacaccgg
    15601 agcacgaagg ccttgcgcct gcagagcacc aggttcagcc tcgcagcggc aagctggtga
    15661 ttgtcgaatc cccggccaaa gctcgtacgg tgggtcgctt cctcggaaag ggttacactg
    15721 tccgcgcctc ggttggccat gtgcgcgatc tgctgcgctc tgagctttcg gtggatgttg
    15781 agcagggctt tcgccccaaa tatcgagtgc ctaatgaaaa acgccaggtt gtccgtgaac
    15841 tgaaagaatt agcccaccac gcggccgaaa tctatctggc taccgaccct gaccgcgaag
    15901 gtgaagccat tgcctggcac ttgcttgaag ccgccgccat tgacccgcaa atcactcgcc
    15961 gggtggtctt tcatgagatt actgaaggcg ctatctcaga agccttttct catccccgtg
    16021 aaattgacat ggaccttgtc aatgcacagc aagctcgccg tatcttggac cgcctggttg
    16081 gctacagcat cagcccgctc ctctgggaaa aagttcgcag ccgcctttct gccgggcgcg
    16141 ttcaatcagt tgctttgcgc ctgattgttg agcgcgagcg tgaaattgaa gccttcatcc
    16201 cccaggaata ttggacgatt gaggctgagt tcaaacccga aggtggcaaa agcacctata
    16261 ccgctcgcct ggcaaaagtt gatgagcaag atccggtact gaacagcgaa tccgttgtcc
    16321 agccgctggt caaggacatg gaggctgccg cttaccagat tgaccgcatt aagaagagcg
    16381 agcgccgccg taagccatca gcgcccttta tcaccagcac catgcagcaa gaagcctctc
    16441 gcaaactggg ctacaccgcc cgccgtacca tgtccatcgc tcaacagctt tatgaagggc
    16501 tggatgttgg cgagggtggc agcaccggcc tgatcaccta catgcgtact gactcgacca
    16561 atgtcgctga agtcgctata aacgaagtcc ggcagtttat ccaatccaac tacggcgccg
    16621 attttctgcc tgccacacca cctcagtata agacccgcgc tgcttctgcc caggaagcgc
    16681 atgaggcagt tcgccccacc tcggtgctgc gcagcccgga taaaattaaa gcctatctct
    16741 cgcctgaaca attcaaactc tatcaactgg tttggcagcg ctttgtcgct tctcaaatgg
    16801 aagccgctgt ttacgatacc gtctccatag aaatcggggc cctgactccg gcgcatcgct
    16861 acctcctgcg cgcttctggt caaaccctcc gtttccctgg ctatctggtg gtctatgaag
    16921 aggccaaaga agaagatcag aaacccgaag aggaagaagc caacgttcgc atcccaccgg
    16981 gtctagaaga aggccagaaa caaaaactgg ttcgcctcat ccccgaacag catttcaccc
    17041 agccgccacc tcgttattca gaagccacac tggttcaggt gctggaagag tttggcattg
    17101 gtcggccttc aacctatgcg cccatccttt ccaccattca agagcgcggc tacgtgattc
    17161 gggaagctaa gcgtcttttc ccgaccgaaa ccggctgcct ggtcagtgat ctgatggttg
    17221 agcatttccc caatattgtg gatatgcact ttaccgccca tatggagtcc gatctggatg
    17281 atgttgccgc cggcaaccgt gaatgggccg atgtgattgg cgagttctac cgcgattttg
    17341 aacccaccct ggagcgtgcc cgctccgaaa tcccgatcac caaagccgaa ctggaaaaaa
    17401 ttggccgcgc ctgtcctgaa tgcggcaaag atctggtcat ccgctggggg cgctatggca
    17461 aattcattag ctgcagcggt ttccctgctt gccgccatac cgaagccttc ctggtcaaaa
    17521 tcaacgtcac ttgccccgaa gacggcggcg atattgtcga gcgcaagacg cgcaaaggcc
    17581 gcatgttcta tggctgtgcc aattacccca actgcacctt cacctcctgg aagcgcccta
    17641 tcccggttcc atgccccaag tgcggcggca cccttgttat tgccaataaa cgcgagatgc
    17701 agtgcatcaa atgtcaggaa accttcctgg tagaagaggt tcccgccgca gcagaatcgg
    17761 cataaacctt gcaacaccat caaacacccg gcaaatttcg ttgaccattc gtgttgcacc
    17821 tttttgtttt ttgttctaca atagatgcat caaacatggt ggaatacact ttgctcatcc
    17881 gttgatccga ctttcccgta ggagcagata agatggaaaa aatcaaatca cttctcaaca
    17941 acccactgta cgccggcatt gtcggcctga tcattgggtt gatcattggc ttgcctctcc
    18001 tggggtgggg gctttggcct gtgaagtatt acgatgccgc ccccgtcaac ctgcgctcag
    18061 acctgcaaac cgattacctc tgcatggtga ttgatgctta ctcgcaggac accacccagg
    18121 ttgccattgc ccagaagcgt tatgctgaat taggcgaggt tggccccacg ttgctctcta
    18181 ccctgactgc tgcaacctgt ggcgtcggcg tcagtgatgc cgatatcgcc actttccgca
    18241 ccatcgtcgg cgcggcggct cctgtcgaga ccaccgttcc tgtgaccggt gaaacggcag
    18301 cccctatcgt cactcccgag cctactaaaa agcctaccag ctttggctgg gtgattgccc
    18361 tctgtctggg aaccctggta gtgttgggca ttctggccta cttgctgatc ctcaaacctc
    18421 gcaagcgctc aacctctggc cccagcgttg tagagcaggc tcaagaagcc gcccgcagtg
    18481 ctgttcagac cgattttgtt gcccagggtc atgagccgcc agttgcccag tttatgacca
    18541 cctacatgat tggcgatgat ctgtatgacg actccttcag catcgactcg cccactggtg
    18601 aattcctggg tgagtgcggt gttggcatct ccgagagcat tggtgttggc gatcccaaga
    18661 aggtcaccgc ttttgaagtc tggctgtttg acaaaaacga catctccaca gtgaccaaag
    18721 tcttgatgag cgcccatgcc tttgacgatg aaaacacgcg ctccatgctg gctgccaaag
    18781 gcgaacctat tctggttgag cctggtcgcc gggttcagct cgaaaccaag accctcatcc
    18841 ttgaggcccg tgtcgttgat acgaattatg gtcagagcgc cctgcccgcc gaaagcttct
    18901 ttgatcgcct cacgctggaa ctggcgatct ggcccaagcc ccacgcctaa tctgcacaat
    18961 tcctgacggt gaaaaaattt cgggcggcct gctggccgcc cgatttgatt tactcaatct
    19021 tcaaaacttt gaatttgatt gccccgctgg gcgtatccac cgacacggta tcgcccagcc
    19081 gctgccccag caaggcgcgc ccaatcggcg attcgtgcga tatccgtcct ttggccgggt
    19141 tggcttctgt cggccccacc agttgatacg tttcgggcgt aaaatcatcc tcttgaatgg
    19201 tgatccgcgc cccaattgta atcacaccat cagttttgcg gctttcatca ataatcgccg
    19261 cgttttgcag aatctgctcc aattcaataa tgcgaccttc aagaaacccc tgctcttctt
    19321 tggcagcaat ataatctgcg ttttccgaaa gatcgccctg ctgaatagca aaacgcagtc
    19381 gttccgacaa agcctggcgg gcaggcccct tgagttgctc aagttcctgt tttaattttt
    19441 ccagaccatc tgcagtcaaa taatgtactt cagccatgaa ccttatcctg taagagtgat
    19501 tttcagtaag tcccaatcat accacatccc cacgctcaag gcagcatgga tgggtcaaaa
    19561 agcttgtgat attcttctac cgcatagcgg tccgtcatcc ccgcaatata gtcgcaaatc
    19621 gtacgctcca aaccgcgtgc ctcaatcagg tcttgcacgt gtttgggcat tgtggatggc
    19681 tcatcgcgat aggcgttgaa cagctctgac aagacacgtt ccgccttgac cgccatgcgc
    19741 accacgcgat agtgccggta aagattgtta tacaaaaaat ctttcaattg gcggtttcgc
    19801 cggtgcatat cttcatcatg ccctaccagg ttaaagggca tctgctgcaa gtcttccatg
    19861 gaacgaatgg aatgttctct cagccgttta tcggttgttg aaacaatatt ggaaaccccc
    19921 agtccaataa tattgcggat gatcgcatgc cgatcctggg ccgaaagcgc tccgccgcgc
    19981 cagccaatct ctttgctcac aatttcccac cattcaatcc cctcaagcat ctcgggcgtc
    20041 agcatcttgg agcgcaggcc atcatccagg tcgtgtgccg tataagccag ctcgtctgat
    20101 acattggcaa tttgggcttc cagattgcct cgcagattag ggttaaacgc ccgcgcgtcc
    20161 gatttgtcat attcggtttc gtgtttaaca atcccttcca atacctccca gctcaaattg
    20221 agccccggaa aatcaggata ccggttttcc agctcggtca caattcgcag cgagtgctta
    20281 ttatgctcaa agccgccgtg gtcgcgcatt aaccgcgcca gggttgattc gccagaatgt
    20341 ccaaagggcg ggtgccccag gtcgtgggcc aggcaaattg tctcggtcaa atcctcgttg
    20401 ccgcccagtg ctcgcgccag ggtgcgccca atttgtgcca cttccagcgt atgcgtcagc
    20461 cgggtgcggt aataatcccc ttcgtaattg atgaagactt gcgttttgta ctctaaccgc
    20521 ctgaacgccg tggtgtgcag aacccggtct cgatcacgct gaaaacatgt ccgaaattcc
    20581 ggttcgtgat caggatataa ccgtccgcgg gttttactgc tgtgcgcgcc gtagggcgcc
    20641 agcgtttgat cttccaactg ctcaagttgc tcacggctga aaatcatctg gacctcctga
    20701 aggattgaat atcagcgtac cgggcaagtt gccggccagg gcttgttcaa tcaggcccct
    20761 ttgcaggcct gaaaaaatac agattttgac gtaaggttcc tcggtcacca gttgaatcat
    20821 tcgctcaacc ttggacgcca tcccccccgt aacatccgcg ctagccgagc cttgcagcgc
    20881 ggcggtcagc gccgggtgag tttgcggggt gatcaaatcc ataattcggg tgcagtctgg
    20941 gtaatctgcc catacaccgg gctcaacccc ggcaattaaa atccgatccg ggtggagctt
    21001 ttgggccaga taataaaatt gatcttcagt ggataaaatt gttccaccca tcgcctggtc
    21061 aaaaatcaca tcccccgcca ccaccgggat cagtccggca tctaaagccg cttgtagcgg
    21121 cccaatatcc cacgaaacaa tttcccgctg gttggcggta atacccgccg aacccgggaa
    21181 ggtgatgacc ggcagttggg cctttgtgag cgcctcaacc actttttggt tcaatgtccg
    21241 ggcagccagc cagacttcag caaagccctg ccacgcctca ccagtccgaa caccgtccat
    21301 cgtcttgtga tttttggcag catgatgtcc aaatgacccc gacccgtggc caatgatgat
    21361 ttgtatatct ggattttggc ggcaaaccac agcaatttcg cgcgccaggc gggggagcgt
    21421 tccgcgccgc agggtgttgg ggtgcgcttt atcggtaatc aatgagccac ccagcttaag
    21481 gaatacgagt tctttcatag tagattattt cggtttggat gaatggttgt ggaaatcaca
    21541 cgcacagctc cggtcttgag cagcgcgttg gtaacatggt cagccgcagc ctcttccacc
    21601 tgggcaatca gattgccacc gcgcccgccg ccggataatt tgccaccgag tgccccggct
    21661 tctatcgctg cctctaccag cctgtccagc tttggattgg acacgccaag ctgctgcagc
    21721 aactgatgat tctgattaag cagcgccccc agcgattttg ccggtccgtt ctcgatgcac
    21781 aaccgtgcct gctgcgccag actgccaatg gcgtcaaaaa tggcgtcaaa agctgccgga
    21841 gatttttccc agccgcggcg cacatctgca acaacctcgg ccgttgaact gggctgtccg
    21901 ctgtcagcaa tcaacagatg aacgggttcg gcaacattca acgcctcaaa gggctggcct
    21961 ttgacaaaat agattggccg ggcgtaggtg atgaccgtat tgtccacccc cgaaggtgaa
    22021 ccgtgctggc gcttttctga ttcgtaggcc aactggcata ctgtcatctc gtccagtggg
    22081 tagcccaaaa attgtgtcaa agcccggatt aacgccaccg ccacagccgc gctggatccc
    22141 agacccgccg ccggtggaat tgtactggtg attttgatct gcatggcagg tagtcttttg
    22201 acctgtatgg ccctttgcac taggtgaata atccaggcaa tcggctgatc gtctggtaat
    22261 tcactcaacc ttgcatccag tcctactgcc ggggcttcaa ttttgatctc gccgctcggg
    22321 gcgttgggcc tggcagaaat tgtcgcccgc actgccacgt ctgtaactgg cgcagcaatt
    22381 gccgggcgtg catacactgc cgcatgttca ccaatcaaaa tgcattttcc tggagcggtt
    22441 gcagttgtgg caggcatgat gatgctaatc aaacagagca aatataacca taaccgcaat
    22501 gccatacagg atcacggttg cttgcagcgg gcggtccgat aacagcagct cttcgggcgc
    22561 tcccccggcc tctttaacct ggatcagata gaggtagcga aaaatcccat acagcacaaa
    22621 cgggatcgtc agcatcatgg tgtgattttc tggcaggttc ggcgccgaga aggtgtacag
    22681 gctgtatgcc acaatggtcg tgccagaaac aatagtgatc aactggtcca gcagagggat
    22741 cgtatagcca tcaagcaccc ggcggtgagc attggctccg tgcagcagca gcgtcagctc
    22801 tgcccggcgt ttgccaaaac ccaggtagag cgctagaagt gtggtcacaa catacaacca
    22861 gggcgagaag cgttccacgg tgatgagggt cacgccggca gccacacgca gcacaaaacc
    22921 ggcggcaatc accaatacat caatcagcgg gatatgcttc aaccattgcg aataggccag
    22981 gttgaccagg aagtaggccg cggcaataat gccaaacccg ggtgagagcc accatgccag
    23041 cggcagcgtg attacgacaa ataaaattgc cgcggtcgct gccaccggaa ccggcagttg
    23101 cccggatgca attggccgca gcctcttttg cggatgctgc tggtcggcct tgatatcaaa
    23161 aatatcattg atcaaataaa ccgcgctgga cagcaggcag aaaagcagca tgcccgccac
    23221 cgagcgcagc acagacggta gcaccagaaa ctgcacatca aaaataagtg cggccaaaac
    23281 aaaaccgttt ttaggccatt gtttaagccg catggcttta atcagggcgc gaatcatgtt
    23341 gtaatcatat cacaagttgc caatctatta gtacaataag acctatggag aaacagcgtc
    23401 tcgatctatt gttggtagaa cgcggcctgg ccgagagccg ctcgttggcc cagcgtttgg
    23461 tgatggcggg gcaggtgcgc gtcaatggtc aggtcgaact aaaatccgcc gccaaagttg
    23521 ctttgggcgc ggatctggta gtcgatcatg gcccgccctt tgtttctcgc ggcggcgaaa
    23581 aactggctgc ggcgctgtcc gcctttggcc tgaccgattt gcagcaccgg gtatgcgtgg
    23641 atgttggcgc ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtatgcg
    23761 tggatgttgg cgcctcaacc ggcggattta cggactgcct gctccaacac ggcgccgccc
    23821 gggtctattc tgtggatgtc ggctatggcg aactgcactg gaagctgcgc aatgatcccc
    23881 gcgtggtggt gatggaacgt accaatgccc gctatgtcac tggcttcccc gagcccgtga
    23941 atatgattgt gattgacgca tcattcatct ctttgcgaac aattttaccg gcggtcaaaa
    24001 gctgggtctc gccagaaaac ccctgcataa ttgtagccct cattaaacct cagtttgaag
    24061 ccgggcggga ggacgcggct cggggcggcg gtgtcattcg cgatcctgct gtccaccgcc
    24121 gtgtattaga gcaggttatt gaatccgctc aagaattggg ctttgtcctg cacgggctca
    24181 tccgttcacc gctccttggc cccaaaggca acattgagtt tctggtgcat ctctcttatc
    24241 ccggaactgg cggtgttgat tcgaaattgc tggtggattc tgcccttgct gaaaacaaaa
    24301 ctggaataca atcatcgata gaagattctg cggaggtgaa cccatgaaag atgcttacat
    24361 tcaaaaactg cttggggagc aggaagtggt tttgctggca acccgtcagc actggtttat
    24421 gttgtttggc tccatcatcc tcgaaattgt gctgatcctg gtcatcatcg gggcggcagt
    24481 tctcatcaac gttttatggg cgtcgttacc attccttggc agtctaatta tcattgtcct
    24541 gccggtgatt ctggttgcta ttctcctggt taccctggtt cgcgacatac tggtctggtc
    24601 aaatcgcaaa tatattgtga ccaatcgacg ggtaatccag gtcaacggca ttatcaacaa
    24661 aaatgtcatt gactcatcgc ttgaaaaagt gaatgacgtc aagatgattc aatctttctg
    24721 ggggcggctc tttggtttcg gcaatatcga aattttgacc gccagcgaaa tgggggtgaa
    24781 tgtcttccgg cggattggca accccattcg tttcaaaatc gccatgttga acgctaaaga
    24841 gcagttgggt catgacgaaa atggcatctc ccatgcgcct gccacccgca gcgtgccgca
    24901 gctcattttt gagctggacg agcttcgcaa gcgtggtctg atcaccgaag aagaatttca
    24961 acagaagaaa aaagacctgc tcagccaaat gtagctcggg tagggcggat accgcccact
    25021 tcttacttta tggttctctc gccattgtcg agagaaccat aaatgccatc ctctgaatat
    25081 tgaggtttcg gctgataata gggtatactt gccccgatta tgactactgc ccatattcgc
    25141 aatttctgta tcattgccca tattgatcac ggcaagtcca ccctggcgga tcgcctgctc
    25201 caaataactg gcactatctc cgatcgcgag atggtcgccc aggtcttaga ttcaatggat
    25261 ttggaacgtg aaaaaggcgt gaccattaaa gcctcggctg ttcgcatgaa ctacactgcc
    25321 caggatggtc aaacctacga gctgaacctg attgacaccc ccggacatgt ggactttaat
    25381 tacgaagtca gccgggcgct ggaggcttgc gaaggcgctc tcttgattgt ggatgctacc
    25441 cagggcatcg aagctcaaac tctggcgaac ctttatcttg cgcttgaagc cgatctcaaa
    25501 atcattcctg ttttgaataa aattgacctt ccctcatcgc atgccgatga ggttgcaaaa
    25561 gatctggaac atctgcttgg cattcctgct gattccattt tgcaagtctc tgctaaggac
    25621 ggcaccaatg ttcccgctgt tttggaagcc attgtgcagc aggtgccgcc ccccgcgggt
    25681 caacccgaag gtgtgttgaa agctttgatt tttgactcgc attacgattc ctataaaggc
    25741 gttgtagcct atgtgcgggt tgtcgaaggg cagatgaaat ctaccgatat gctgcacctg
    25801 atggcaacca atattgatat gcgcccggtt gagttaggcg tttttgcgcc tgacatgcgc
    25861 ccggttccct ctcttcaggc aggtgatgta ggttacgttg ctaccggcct gaaaacagtt
    25921 tcagaatgtc acgttggtga taccattacc gccgcgacgc agcccgccgc cgaaccgctc
    25981 ccgggttacc gccgccccaa gccgatggtt tttgccggca tctacccggt cgaaggcgaa
    26041 gactacgccg atctcaaaga agccctggaa aaactccagc tcaacgacgc tgctctggtc
    26101 tatgagcccg aaacctctca ggcccttaat ttcggcttcc gttgcggctt tctgggcctc
    26161 ttccacatgg aaattatcca ggaacgcctg gaacgcgagt atgacctgga tatcgttgtc
    26221 accgccccct cggtggaata cgaagtcgta ctggtaacgg gcgaggtcct cctgatcgat
    26281 tctcccgtcc aactgcccga tgaatccacc attgccgaaa tccgtgaacc ctggatgcgg
    26341 ttggaaatca ttacccccac tgagttctac ggcaccatca tggaactggt catcaaacgc
    26401 cgcggcgcct tcaagagcca ggattatcct gcccccaacc gggtacagtt agtctttgaa
    26461 atcccgctgg ctgaattgat tgttgatttc tttgacgacc tgaaatcccg cacccgcggc
    26521 tacgcctcga tggactacgc cgtggacggt taccgtcccg aaaatcttgt caagttggaa
    26581 gtcatggttg gcggtgaacc cgtagatgcc ctggctgcca tcgtccacaa agagcatgcc
    26641 taccataaag gccagcggct ggtgaccaag ttaaaagagc ttatcccccg gcagttgttt
    26701 gatgtcgcag tccaggccag cgcgggtggc cgcatcatct cccgcgccaa cgtcaaagcg
    26761 ctgcgcaaag atgtgctggc gaaatgttac ggtggcgata tttcccgtaa gcgcaaactg
    26821 ctggaaaagc agaaaaaggg caaacgccgc atgaagatgg tgggcagtgt ggaaatcccc
    26881 caggaagcct ttatggctgt tttgcgcctg gaggatgaat gacccaatcg ccgcccacgt
    26941 ctccctggcg gcgcgtgggg cgctggctgc ctggcgtttt aatcagcctg gttgccatca
    27001 ttgccttatc gcaatttgtc aaagacggcg cctccttacg ggctgctttt gcccagatca
    27061 atctactgta tctggcgctg gtcgttcttg gaaccctggc cttcctcttt gttcgcgccc
    27121 aggcctggcg cattttgctg gggggtaaac ccaccatcat tcagaccttt tttgccatca
    27181 acgaaggtta tttgcttaac aacgttctcc cattccgcgc cggcgaattt gctcgggcta
    27241 tctttttggg taaatccacc ggcctcggaa cgttccacat tctttcaacc atcgtcttag
    27301 agcgcatttt tgatgttgct tttgccgccg gattactgct ggcaaccctg ccaatggcct
    27361 tcgccatgga gcaggcgcgc caggtcgcga tgattgccct ggcgctggtc gtggtcggtt
    27421 tggttgtcct gttttggagc taccgcaacc gcgccaggct cgaagtctgg ctggataagt
    27481 ttgccggtcg ctggtctttt gcccggcgct tcatcctgcc gcagttctct cgcttgttac
    27541 agggctttga agttctcagc cgcccatccc aatttctggg cgccttgttt tggattgcca
    27601 tttcctgggg tatagcagta ttcaactact gggtctacat gctttctttt gcgccgcacg
    27661 cgccgttctg gtggggcgct tttgccgatt caattctcgg cttgagcatt gccatcccat
    27721 ctgctccagc tgcgctgggt gtttttgagg ctgcccttgt tggcgccctg accattcttg
    27781 gcctgtccga aggcccggcc ctggcttacg ccatttcact tcatgccgtt cagtttgtga
    27841 ccactggcat tctgggcgtc tggggccttt tacgcgaacg tcaatcaatt caaggggttt
    27901 tggatagttt ggagcgtaag ccaacaccac agcaggatgc ctgaatgaac attctcattg
    27961 ccctgacgta ttaccgccca cactactccg ggctgacaat ttacaccgaa cgcctggcac
    28021 gcgccctggc ccagcgtggc caccgtgtga tggtgctgac ttcacgcttt gacccccgtc
    28081 ttcccgcccg cgaggttcgc gatggcgtag aaatataccg cccccgtgtc ctgatgcgca
    28141 ttagcaaggg cgtcatcatg ccagccatgc ctttttgggc catccgcctg atccagcagg
    28201 ccgatgtggt taatttgcat gttccccagt tagatgccgc cccaatttcg ctcctcgccc
    28261 gtctgctgcg ccgccccgtg gtactgacct accattgcga tttgcatcta cccaaaggtt
    28321 ttgttcataa ggttgccaac tgggtctcca atttagccaa ccatatcacc gctcgggccg
    28381 ccaatcggct ggtcaccaat accctggatt atgcnnnnnn nnnnnnnnnn nnnnnnnnnn
    28441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnnnnn nnnnggcccg ggtattattt cttggtcagt atcaaaatgt ccttggtgaa
    28561 gaagaatatg cccgccgcct tgctccgtta attgatcaga ttggctccgc ctggaaattt
    28621 ctgggcgtgg ttccttcaac tgccctggcc gcgtttttcc ggctgtgtga tctgaccgtc
    28681 cttcccagca tcaacggcac tgaatcgttt gggattgttc aggttgaatc tatgatgtct
    28741 ggaacgcccg ttgttgccac agacttgccg ggcgtccgcc agccggttcg catgactggc
    28801 atggggcgga ttgttccacc ccgcaacgcc ctcgccctgg gtgaagcgat catcgatgtg
    28861 ttggatcatc cggataaata tcagggagac cctgctcaga tagcccggat gtttgccccc
    28921 gcctcgattg ctgaacaata cgaaaaagtt tttggagctg tgctgtcatg acctcgaaag
    28981 atcacctctg gctgcagctc aaatccatgc cctattttcg cggcctgctg cgcgccgttg
    29041 aagcgcgatt ctatgacgac atcactctaa ctggtccagt gctcgatctt ggctgcggcg
    29101 atggccattt tgccagcgtg gtttttgatc accccctgga tgtaggcctg gacccctgga
    29161 cggcgccatt ggttgaggct gccggacgcc gcgtttacaa tctgccaatc caggcggccg
    29221 gcgctcaaat gcccttccca gatgcttatt ttaacagtgc catcagcaat tctgttttgg
    29281 agcatattcc caaccttgac cctgtcctgg ctgaaaccgc gcgtgtcctg aagcccggtg
    29341 ccttgtttgt tttctgtgtg cccaatcatc attttgatcc caacctgagc gttgcaaaac
    29401 tattagatcg gctgggcctg cgcaaactgg cagccaccta tcgccgcttc tttgatcgca
    29461 ttgcccgcca ccataacctc gatgcccccg aaacctggat cccccgcctg gaaaaagccg
    29521 gtttcactgt tgagcgctgg tggcattatt tttccccggc tgctctggct gtgctggaat
    29581 ggggtcacgc ttttagtctg ccagccctgg tcacccgcaa attgacctcc cgctggatcc
    29641 tggcgccgta ccgctggaat ttattcatca ctgagtcgat cacccgtccc tattataacg
    29701 agccgtctcc acaaccggag ggggtttgca cattctacat tgcgcgtcgg cgcagccagg
    29761 agtgatatgg caaatacact tcgcaaatac caatattacc tctcttccat cccgcgcctg
    29821 ttgaccggct ttgttgagtg gcccaaaatt ttgggcgtat ttttgaggtt gtatcgcacc
    29881 acaccctttg aggttcaatt gcgtcacagc ggcgttcgct tttccgcccg cagccccatg
    29941 gatttatgga gcatcaaaga ggcttttatt gaccgtttct acgagcgcta cggtttccca
    30001 attgaaaagc gttggacggt ggtggatatt ggcgctggca ttggcgaata cacgcttttg
    30061 gctgcgacaa cagacacctc aaaccgcgtg atcgcttttg agccctttgc tgaatcttac
    30121 aagctcctgc gccaaaatct ccgcctcaat ggcgcaatta acgtttcggc tgttcccatc
    30181 gcaatcagct ctcgtacagg ccaactgagt cttgaccttt cggcagggga acccttgaag
    30241 attgaaagcc accttgccga ggccgaatca cctacgggca cagccggact ggtggagtgt
    30301 ctgtcccttg ccgatgcttt tgagcgctac gagatcgaac actgcgattt gctcaagctg
    30361 gactgcgagg gcgctgagta tgatatttta ttttacgctt ccgctgaaac cctcagccgg
    30421 gtaaagcgga ttgtaatgga atatcacgac ggaaaaacag cgtatagcca taaggatatg
    30481 gagcatcatc tccgtgatca tggctaccgg gttgaaagcg ttgtcaatgt tgtccacgac
    30541 gacattggtt atttgagcgc cattcggcct taggagggtc tgtgggcacg gaaacccagg
    30601 aacccagcat tctggattat cttaagtcaa aactgaccct ctctgcctca gcagctcatc
    30661 cctggccctg gcgggtattg ctggctctgg ctttggcttt gttggctcaa nnnnnnnnnn
    30721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaccttctgc tgtggttggc tgctgttggg
    30841 tccatggtcg ctgcgctttg ggcgcctcgc attggctttg ccgatcgcct cgccagcttg
    30901 tggggttggc tcacgcggcg tcaatggcaa atccgcatca ccccctggat gctgctctgc
    30961 gtagttgttt ttgctgcagc aatctttttt cggttttacc ggttggattc tgtcccccct
    31021 gaaatggtca gcgatcacgc cgaaaaatta ctggacgtga gcgacgtctt atcgggcaac
    31081 accagcatct tcttcactcg caatacgggc cgcgaaccct tccaaatgta cctcacggct
    31141 ctggtcagcc tggtattcgg cacaggggct tccttcctga gcttgaagct gggcactgtg
    31201 ctgtgcggtg tactcacact gccctatctt tatgcacttg gcaaagagct tggcagccgc
    31261 cgcatcggtc taatcgccct ggcctttgcc ggaattgcct attggcccaa tctcatctcg
    31321 cgcatcggat tacgcttcac gctttatcca ttcttttttg ctcccacgct ttattatttt
    31381 ttgcgcggaa tgcggacatc cagccgcaac gattttattc tggccggact cttcctcggc
    31441 cttggcctgc acggctactc cccgtttcgg attgttccat tgctgctggt atttgccatc
    31501 gggttgtttt atctgcatcg agtttctaaa taccgccgca gcgaatcgat tgccggcctg
    31561 gctatcatca ttttggtttc ggtggttgtc tttctcccgc tgctgcgctt cttgctcgaa
    31621 aacccggcaa ttttctccta ccgtatgcta actcgtttga ccggcatgga atctcctctg
    31681 cccggtccaa tctggcagtt attcttcaca aacttatggc gcgccctgac catgttttca
    31741 tggaacaacg gcgaggtctg ggtgatttct gtgatgggcc gtccagtttt agactgggta
    31801 gcgggcgggc tgttcaatct cggcgttgtt gttctgctgg tgcgttatat tcagcgccgc
    31861 cactggctgg atttgttcct gcttctctcc attcccattc taatgctccc ttccattctt
    31921 tcatttgctt ttccagccga aaacccttgc ctcaatcgca ccgccggcgc gatcatcccg
    31981 gttttcctca tcgtcggctt tgcacttgat ggagtgatga aatcgctcga atcccacctg
    32041 gcgcgcctgg gttcccggct cgggtggctg ctctttgctg gcttgttttt aatttcggcc
    32101 gctcagaatt atcagttggt ctttaaccag taccgtagtt tttatgaaca ggcttcgctg
    32161 aacacttccg agatgggcca ggtcatgggc gcctatgccg aatggtcggg ctctcccgaa
    32221 accgttcatc ttgtcggcta cccctactgg gtggattctc gcctggtcgc aatcaatgcc
    32281 ggttacccaa ctctggatcc cggcatcatg cccgaaaatt tgccgcaggt ggccaccgac
    32341 aaccgcgccc aattgtttat tttgaaccca tacgataccg atggggtggc agcattgaat
    32401 ctgctgtatc ctggtggtac actaagaaat tacgattcaa aattcgatgg caaagatttt
    32461 ttgctgttct acgcgccagc caaaatgcca taagccattg attgaggagc cctcagcatg
    32521 gatgaccccc tgacacctgt ccaagtaact gaaaattcca ccccttcccc gtctaccgaa
    32581 aaaccacccc ggcggaagtt ctcctggggc tgggcctggg atctattact ggtggtcatt
    32641 ttactggttg gcggttactt ccgcaccatt ggaattaact gggattcaaa ccaacatctt
    32701 caccctgatg agcgcttcct cacgatggtg gaaacttcaa tccagccggt tgccagtctg
    32761 gcagattact ttaacactgc cacctcaacc ctcaacccca acaatgttgg tcatggatac
    32821 tttgtttatg gcacactgcc aattttcctg gtgcgctatg ctgccgaggc gctcgaccaa
    32881 accggttatg atcaagtcta tctggttggt cgggcttttt cagcatttat tgatctatta
    32941 acggtcttcc ttgtttttgt aatcgctctc cggctttacc gcaacactcg cctggggctt
    33001 ttggcggctg ctttttcagc ctttgcggtg ctgcaaattc agctctcgca ctttttcaca
    33061 gtcgatactt tcgccaactt tttcacctat ctggctttct attttgccgt tcgcgtcatg
    33121 accgccgata cccccggcac acccgtctcc tccccgacag aaaccgccga agccgacatg
    33181 cctccagcac gcccttcacc ctgggcctgg atgcagttgt cctggcgcgg ttggggcaac
    33241 tatatcctct ttggtctggc gcttggcatg gcggttgcct caaagatcag cgccgcgcca
    33301 ttagcccttc tactacccct ggccgcggta ttgtttatct cccggatccc aaaaccccag
    33361 cagcgtgacc agatcattat tgccttccgc cacctcgttc tggcagccct catggcaatt
    33421 ctggtctttc gcattttcca gccctacgct tttgatggtc ttggattgaa tcccaaatgg
    33481 gttgccaatc tgacggaact gcagcgccag agcaccggcg atgtcgattt ccctcccgcc
    33541 ttgcagtggg ctcggcgtcc cattactttt ggctgggaaa atctgaccat ctggggatta
    33601 gggctgcctc tgggcttgct ggcctgggct gccttcatct ggatgggttg gcgtattttc
    33661 cgcggcgaat ggcgcaacca tttactcctt tggggttgga ccgggctgta cttcctctgg
    33721 cagggtactg cttttgtcag ctcaatgcgc tacctcttgc ccatttaccc ctcgctggca
    33781 atcattgccg cctggggcat ctttgctctc tgggaagctg gtaaaaagaa gcctttaccg
    33841 cgttggaact ggcaaaaaat tctttcagtt gcagccggag ctattgtgct ggtcagcact
    33901 tttgcctggg catttgcctt cacccgcatt tatacccgcc cggtttctcg cctggaggcc
    33961 agctactgga tctaccaaaa cgtccccggc gccatcaatt tgagaatgac aaccgccgat
    34021 ggtgattatc aacagcccct gggcagtctg ggcgcgctga ctttgatgtc agaccacccc
    34081 ctcacgctca cttttgatgc ccccgccgct gttacgctgc aggatatcac cattacccga
    34141 ctgagtgatg cgcagtggtc tgctgaccag cgcactctgt cgttcagcat ttatgccgcc
    34201 aacagccccg attcaacggt attagccagc ggcatgttgg cctcctctta tgctgttgaa
    34261 caatcgacaa ccataccgct gtccattccc ctgacgcttg aacccgcaac agaatatgtc
    34321 atccagttca gcgttatgga acccggtgcc atgatgcgtc tggcgggtta tctggatctt
    34381 ggtttgcagg atggcgaacg tttatatcgc caccgcgcag cggaatttgt tcaattaatc
    34441 accaccgaac aatcgtttca ggtgccctcg ttccggattt tggaagccgg cacactgaac
    34501 ggcatctggc tggaaaatgt tgcggattgg gaatcgcagc ccgaccccaa aaccttgcga
    34561 gtctcgcttg cgctccccgg ccaggaatcc cagccccttg ccgtcgctca aattcaagat
    34621 acattcctgc ggcaaaatga ttttcgcggc gaatcctact ggttagcgtt tgatcccccg
    34681 gtgcaaatca tccaggatgg cgtttatacc ctgacgtttg aattgcccga aggcaccggt
    34741 gcccttgcgc tcaccaacag taaagtagcc atcgaatctt cctgggatga tgccgtgccg
    34801 ttatcggtcg ggcagggtgg accctacgat gtcaactggg ggccctaccg cacggatttg
    34861 aattttgaaa tgtactggga cgattcagcc gacaagctcg aacgctttat cacaatcctg
    34921 aacaacacag attatatttt catgagttcc aatcgccagt ggggcaccat cccgcgtgtg
    34981 ccagagcgat accccctgtc tgcctattac tatcgacatt tgctgggttg ccctgaagat
    35041 aaagacatcg tctggtgcta ttccgctgca gaacccggta tgttccaggg tgaccttggt
    35101 tttgaattga tctatgttgg cgcatcttat cccaatttgg ggagtttgga attcaatacc
    35161 cagtttgccg aagaagcctt cagtgtttac gaccatccca aggtcatggt tttcaagaaa
    35221 acagccgatt accagcccac cgcaacccag gccttgctgg aatcggttga cctttccaat
    35281 attgtgcgcc tgacaccgcg ccaggctggt gattacaaag gcaacctgat gctgcctgcc
    35341 aaccgtttgg ttaagcagca gtccggcggc acctggtcag aactcttcaa taccgatgcc
    35401 ctccacaaca aatatcctgc tctggctctg attctctggt atgtggtgat caccctgctg
    35461 ggctggctca tctatccatt tgtgcgcctg gctatgggcg gtctgaccga caaaggctat
    35521 ccgtttgccc gcctgatcgg cctgcttttg cttgcctggg tcacctggtt ggctggttct
    35581 gccggaattg atttcagccg tctgaccatc ctgattatat ttgttctcct gtcgctcggc
    35641 agcctggccc tggggtggct gcagcgcgat gccttgcgtg aagaatggcg tacccgcaag
    35701 cgctacttta tctctgtcga actgattggc ctggcctttt ttgtcctgtt cctgctggtt
    35761 cggattggca acccggatct ctggcatgct ttcaagggtg gcgagaaacc catggatttc
    35821 tcctatttca acgccgttct taagagcacc acctttcctc cgtacgaccc ctggttctca
    35881 ggagggtacc tcaattatta ttactatggt tttgtgatca ttggtgttcc ggtcaagctt
    35941 ttaggtattg ttcctgccat cgcctataat ttgattctgc cgacctggtt cagcttgatg
    36001 gcgctcggcg cttttagcgt gggctggaat ctggctaact cagttaaaaa acctgccgag
    36061 acagaccaac agcctcaacc cgttgctgct ttagatattc tgctgtcaga gcctgccgac
    36121 acagaatctc caatagcgcc tgccattgcg ccttctgaat acatagactc tcctgcgcca
    36181 gcctcgcccg ttgccgaatc tacgcaggtg ccggtggccc ccactaaaaa acaccgaccg
    36241 gccacttgga acctttatat ggctggcata actgccgccg ttgccatttt gatcctgggc
    36301 aacctgggca ctattcgcca gatttggcac agcattcaac gtttggcggc ccctggcgcg
    36361 gtcatcgaag atgctacctt tgtccagcgc ctgacctgga cctttgatgg tctggcaaaa
    36421 tatatggacg gcgaaaaact gcgcgtggga actggcgatt ggtactgggt tcccagccgc
    36481 gccatccccg gcgaggccat taccgagttc ccgttgttca ctttcctgta tgccgaccct
    36541 cacgcgcatc tcatcgcact gcccattacg gttctggcaa ttggctggac tttggcggtt
    36601 ctgttgggcc gctggcgctg ggctgaccgc cggcgggcgc tggcatttgg cgccagcatg
    36661 ttgctcggcg gagtggtttt gggcgccctg cgtcccacca acacctggga tatgtatacc
    36721 tttttaccat tgggtgccat tgtcctgggc tacaccgcct ggcagtacgc tgattggccc
    36781 attcgctggt tatccgattg gccgcaaaag ttccaacgtt ttgttgttgc tcttgccagc
    36841 atggctttgc tggtcatcct tgcgctgctg ttctacgcgc cattcactca ctggttcggc
    36901 acagcttaca attcctttgc cctctggaag tatgaccata cacccttctg gtcttacatc
    36961 acccactggg gcgttttcct ctttgtcctg atcggttgga tgtattggga gacgcgtgac
    37021 tggctggcaa aaacccccgt ctctgccctc aaaaagcttg aaccccatct gatctggttg
    37081 atccccgtcg gcctgattat ccttgccctc atcattctat tgatgtctgg cattacgacg
    37141 gcgggcctgt ccgatgaagt tctggtgcag ctaaaaaccc ctggcgttgc agttgcctgg
    37201 ctggtgatcc cgatgatcct ctgggctggc attctcattc tgcgccccgc tcaacctgat
    37261 ggaaagcgca tggtgctctt catgattggc accgccctga ccctgacact ggttgtggag
    37321 cttttagtcc tggtaggcga cattggccgc atgaatacag tcttcaagct ctacttgcag
    37381 gcctggacac ttctctccct cagtgctgcg gcagcccttt actggctctg gcccgagatt
    37441 aacctcaact ggacgccgcg ctggcgttcc acctggcagg tggtatttgc cctgctgatc
    37501 ggcagcgcct tactcttccc gctcgttgcc ggtgctgaca aaatccgcga tcgaatgagc
    37561 aaccaggctc cccataccct ggatggcatg gcttatatgg aatacggcag tttctatgaa
    37621 aacggcgtca atattgaatt tgatgaagat taccgcgcca tccgctggat gcaggaaaac
    37681 gttaccggct ctccggtgat tgtcgaaggc cgggttgggg agtatcattg ggggaaccgc
    37741 ttcaccgttt acacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttgtcg
    37861 aaggccgggt tggggagtat cattggggga accgcttcac cgtttacact ggcttgccaa
    37921 atgtcctcgg ctggaactgg catcagagcc agcagcgcgc cggggccacg cagccctggg
    37981 tggggcagcg tctcaatgat gtgatgctgt tctacactac ggttgaacgc agtgaaaccg
    38041 aaaacttcct ggaacgctat aacgtcagtt atattattgt cgggcagttg gaacgcgcag
    38101 ctcaccccgg ccccggactg gataagttcg tccagtggga aggtgatctt tggcaggaag
    38161 tttatcgaga cggacaaacc gtgatctatg aggtgattcg atgacccctt cagaattaat
    38221 ctaccacctg accatacagt ccgattggca aacggctctc caaatcggcg aataccgcgg
    38281 caatacgctc gaaaccgagc attttatcca ctgctccacc cgcacccagg ttttggaagt
    38341 tgccaaccgc atcttcaaat ctcgcctcga tctgctcttg ctggaacttg accctgcgct
    38401 ggttgttcca gaaataaagt gggaaatatc cgagaatgac gatacttacc cccacatcta
    38461 tgggcctttg aatttgaacg ctgtccgtca tgtctgggcc atgccgcccg agtcagacgg
    38521 cacgtttgtc tggcctgtcg gggccggaga atagaccaga tgctggcgtt tttatcctgg
    38581 tatgtgttaa tcactgtggt ggggttgttg gcattcccca tagcctaccg catatttacg
    38641 cgcctgccag atcgcggcta tgcacttgcc cgcccgtttg gcctcctcct gtggggtttt
    38701 atcttctggc tgttggcttc tttgcatatc ctccagaacg atctgggtgg cgtcttgctg
    38761 gccctgctcc tggtgctggg tgccagcgcc ctggttattc gaaaagaatg gcaggatttc
    38821 attggttggt ttaaacccca cctgaaaatc tggctgacca tagaatgtgt tttcctggtc
    38881 ttttttgccg cctgggccgc cgttcgcgcc ttcaatcccg ccatcgtagg cactgaaaaa
    38941 cccatggaaa tggcgttcat caatgccatt ctgcggtctc ccacttttcc gcctaatgat
    39001 ccctggcttt ctggctattc catttcctac tattactttg gctatgtcat ggttgccatg
    39061 ctgacccggc tgacggccgt tgaaccgggc gtggctttta acttggccag cgcgctctgg
    39121 tttgccctca ccgccacagc cgcttatggt gtgttgttcg cactgatcaa ccgctggctt
    39181 cacgaacgta caccattggt tgagaccctc aaaactacgg ctcgcggcct gggcatggcg
    39241 ctgtttggcc cgctgttcct tctgctggtc ggcaatctgg aagggattct tgaaaacctg
    39301 tatgccgtgg gtgccttctg gtccccggcg gcggatggca cgtaccagtc gcgcttctgg
    39361 gcctggctgg atatcgccga attaaaagct gcccctacac atttaccctt cgattggtcc
    39421 gcctggaatt tcttgaagga tggcctgccg catcgccagg ctggctggct catctggcgc
    39481 ggctcccgcg ttctgacgga ttacaatctt tctggcggcg ccattgaaat tattgatgaa
    39541 ttccccttct tctcctacct tcttgctgac ctgcacccgc atgtgctggc aatgcctttt
    39601 gttttactgt gcattgggct ggccttgcag gtttatcttg cccatggtcg cccgtttgaa
    39661 ggcaaatcca tctttgattg gctccgcttg ccggaatttt ggctcaatgc tgtcatcttc
    39721 ggcagtctgg cgttcctgaa tacctgggac tttccgattt atgtagcgct tttcagcgcc
    39781 gcgtttatcc tctctcgtac gcaaagcttt ggctgggcaa cccggcgttt atgggaattt
    39841 atcggtgtgg ccgcggtcct tgggattgct ggcgttgtgc tttatctgcc attttacctg
    39901 ggatttgcct ctcaagccgg tggcattatg cccagcggta tttttgtcac acgcggagcc
    39961 catttctggg tcatgtttgg cgtcttgctg gtacctgtct tgatctggtt attctggtcg
    40021 tggtctcata atggcagccg gcccgcattg aaagccgggg gcaaatttgc tgtatgggtg
    40081 attggcggcc ttgccctggc ctctctatcc cttttagtgc ttgcccccgt cctggaatct
    40141 attgggcgtt caattttgtc cacgccatcc ggagctgcat cgcgcctggc aaacagcatg
    40201 gtattctggg ccagtatgat taacagcctg tttggagctt ccgatggcgg tgcgttgctt
    40261 ggcgcagccc tgcagcgcag aataacctat gtcggcacct ggctgcccct gacggctatt
    40321 gttgtcctgg cctggggtct aatgtccgct ttaaaacgcc gccccgcttc agaagaatca
    40381 acatcccagc acattgcccc tattcaggcc tttgtcgccc tgttagccct cctgggggct
    40441 ggcctggccc tgttcccaga atttttcttc ctgcgggaca acttcggcaa ccgcatgaat
    40501 accatcttca agt
//
LOCUS       DAOPVS010000008        37873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1033_length_37873_cov_51.553625, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000008 DAOPVS010000000
VERSION     DAOPVS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 37873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37873
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1033_length_37873_cov_51.553625"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKL11_01765"
     CDS             <1..242
                     /locus_tag="PKL11_01765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNZ12353.1"
                     /translation="MEKLDRQRKLEYWIKRYGGWAIIFLAFIPNPLFDAAGMIAGALK
                     MPLWRFLIYCWIGKCLKMLAFALGGAALIDLIPWL"
     gene            complement(247..1668)
                     /locus_tag="PKL11_01770"
     CDS             complement(247..1668)
                     /locus_tag="PKL11_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNZ12354.1"
                     /translation="MQPASPQRHSATLWAEISTLTLMGMDTAWVSVWYQYTQAGRTPW
                     WEAIAALFALELGSHFLARVFNFLRIKRSIRIGAFFVWLVLIQILTLNALVYRWQPRG
                     EIFSGLALAEALHVLVITLLALRGVTLATSALDSWGTQGSFRAGILLLGLYFLITSAD
                     KSSLAIIPVGLFLLMGLLSLSATRIADLSVSRGGRVPPFRWRSWLSVAGLAAAAVGLG
                     MLTGQVFGEIIGKLLMGIFTGLLAIVLLAGLLISVPLVGLIQLLIRVIKPEYNRKAIE
                     ALQQSVQQTAATMLDLMEQAAEKIINWEETARLWVVGIILAIAIILIIRRLNAARIRR
                     AEAGEDERRNVSRVQRIPTLPEANRGKPRSAAQVLAAARVRRAYGQLMGLCAALEHPR
                     PPATTPLEFLPALQRLFPEHQEQADQITQVYTQIRYGEYPELEAEVQQVLDGWRQIQA
                     EGRRKLTELRREKLNLRRITPKP"
     gene            complement(1655..2866)
                     /locus_tag="PKL11_01775"
     CDS             complement(1655..2866)
                     /locus_tag="PKL11_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNZ12355.1"
                     /translation="MPIDRWIALLVLFIIAGAFFGLPELVAFATTLGVIVGLARLWQR
                     HALRQVSYQRRISYRRGFPGESSEAQIDVANHKRLPVIWMRTSDSWPTGAPPQDATLI
                     APSHLPERGWVINQYSLRWQQRVTRRFPFQFEQRGVYPLGPVKLESGDPFGLFETERE
                     EEQSDRLVVFPQMLPLKTLRLNADDPFGDRGARRRLFEDPTQPMGVRDYQPGDDFRRI
                     HWPATARTGALQARVFQPVTARVMVVCVNVATTHHIWEGVVPPMLEQLISVSATMIYH
                     AVQDGYSVGLLSNGNLKRADQPFLVSPGRAASQLGTLLETLAGLTAYTTIPFENYLLR
                     AAPRIPLGATLMVVTAFVTPTLAETLVQLKRYRAHTTVISLDEEAPPQIPGVQSIHMP
                     FVVKDEEHAAS"
     gene            complement(2866..3801)
                     /locus_tag="PKL11_01780"
     CDS             complement(2866..3801)
                     /locus_tag="PKL11_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNZ12356.1"
                     /translation="MIQAAAHKIVQNVQKVIVGKDDVIRRLVAAILCEGHVLLEDVPG
                     TGKTTLARALAASLGCAFRRIQFTPDLLPSDVTGLSWFNQKQQEFEFRPGPIMAQVIL
                     ADEINRATPRTQSALLEAMQERQVTVDGVTRPLLRPFLVMATQNPVELEGTFPLPEAQ
                     IDRFQLRVQIGYPTQTEENDILARFRLSDPLVELEAVTTPEEIQALQQERRAIRVEDS
                     VRDYVVRIARATRQNADIQLGASPRATMALFQAAQAWAALQGRAFVLPDDVKAMALDV
                     FAHRLMIAPQAQLRGRSAVELIEDIVSAVPVPVEG"
     gene            complement(3824..4612)
                     /locus_tag="PKL11_01785"
     CDS             complement(3824..4612)
                     /locus_tag="PKL11_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNZ12357.1"
                     /translation="MKIIALMTDFGDRDGYPGIMKGVIWSIAPNAQIADLTHQIEPQD
                     ILAGALTLARAWNYYPEGTVFVGVVDPGVGTERRAIAARIGTRYFVGPDNGLLTISLE
                     QAETAGEPVIIVQLDQAEFWLPNPSRSFHGRDIFAPVAAHIVNGAALEKLGTVMREPV
                     RLTIPQPTATVGGWQAPILHVDHFGNLATGLQIEALEGQGCTIHFGDTEIPLVLTFGD
                     RGPGSLVAMIDSSGYLAVCVVNGNAAQRLKAKVGDMIDVRYRPA"
     gene            4787..6691
                     /locus_tag="PKL11_01790"
     CDS             4787..6691
                     /locus_tag="PKL11_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNZ12358.1"
                     /translation="MPEKIPFRERFQYWLDNQFSRGTGALILWLGILSLAVIVVAGGI
                     IALLGIAPAGGERLSFLEAAWQSLMRTLDAGTMGGDEGLGFRVIMFLVTLGGIFVIST
                     LIGVLTSAVEGKLEDLRKGRSRVLETNQIVILGWSEQIFTIVTELIAANENVEKPCIV
                     ILGKKDKVEMEDEIASKIEDRKNMRIICRSGDPMDVADLNLVSLNQARAIVVLSPEGN
                     DPDSEVIKAVLAVVNHPNRDPEKTFHIVAEIYEPENMDAALVVGRGQVEWVLVGDFVA
                     RVVAQTCRQSGLSVVYTELLDFGGDEIYIKPEPRLTGKTFGEALLAYRTNTVLGIRKP
                     NTMPCLNAPMDQVIEAGDELVVIAEDDDKIFLTLDQPVQIQEAQIVSGHIAPKQPEAT
                     LLLGWNRRGATIIRELDNYVAPDSEVTIVADVDTVGTDLEICCSELTNQKITFQQADT
                     TNRRVLEGLNLRHFNHIILLCYSDSMDVQRADAHTLVTLLHLRDIADRDGTKFTIVSE
                     MLDIRNRNLADVTRADDFIVSDKLVSLMMAQITENKYLNGVFTDMFDPEGSEIYLKPA
                     AEYVQLGKPVNFYTIVETARRRNQVAIGYRLQANSQDAANGYGVKINPDKAEMVTFTD
                     HDRIVVISEE"
     gene            complement(6768..7865)
                     /locus_tag="PKL11_01795"
     CDS             complement(6768..7865)
                     /locus_tag="PKL11_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12359.1"
                     /translation="MNNLDAFIGLLLFMAGAPALVLQFTPNDVRHILLKNKSDKWKFI
                     ANLYWPAVVGAVSTLGVFVFSNPTPCKWAEVGLLVLLIGLTIFVCVKILSYASKRDVV
                     KKQADQAIGAHQKTEAMSNDYFENIRLLGRESKSSGEKLWAIEAVGKIASYRLSRTNY
                     AGNEMEAAIDALRGIILEGRQGSSPEIFHKAIEILNQIIQKHRERPGLSQADLRWSLK
                     ALSDLGQEAIKFPTPDVTIACINVLESSSRPLSPSASQGLSEIASVALQEGVILVAMQ
                     ALNIIGSAVYENGLEDTKLQEILYDYLGLLGAFWAHGESAQSIANRDLNDLKAGGKIN
                     LKELVERAIEHHRETLRFEVADKISKMWSEY"
     gene            complement(7862..8374)
                     /locus_tag="PKL11_01800"
     CDS             complement(7862..8374)
                     /locus_tag="PKL11_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12360.1"
                     /translation="MLPNRIRVLWIEDEARYQLNELMGPLLYRRIYHLSLAENATEAM
                     RLIRTTEHYDVIIVDMRIPPGEDPAWKMLYQDQGNDTSKARLGQYLIEWMLGQMNGHA
                     EQFSVRPNWIQPQQIAVFSAEEPSGMDRIFKMGVKVYQPKDASYPDDILVQLVGRVMN
                     CKEEQDKRGT"
     gene            complement(8378..9904)
                     /locus_tag="PKL11_01805"
     CDS             complement(8378..9904)
                     /locus_tag="PKL11_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNZ12361.1"
                     /translation="MTISDAEVLKLFRQVHPSELPIGFYLVAPDGRFLIASGGVRYLL
                     GMPLEGEINTTIAEFYENPAQRTMLLEQAEERSRQGRYLQGALIRFCTRGGLRVAENT
                     CRALLDPQIGAVVGYYGTLVDRTAEVESKQQEQKLQQRIEDLTTDIGRVLHANSSTLT
                     MVRLAFDPVLALIATELEIPNPKGELSPVQETALLDGQAQKLGGAIRGLIANNPAERR
                     LQALPQLRWEYLEQLAGDLNDYQQVVPVTEARPSFLRRLAADVTAILGEMRPGLAKEQ
                     VREVQRAALNLQRAAALLTATRAYVASIQMEHSLRALRDFATGDLRSGEERVSVSMRS
                     IVDNAVKSLADFASSRGIEIVIRDHSGGAEIAVQERELQRAVANLVHNAIKYSWSRTG
                     GTKPPWVSIEIRANDSEVSLSVENWGVPIPKDEIENNLIFNLGYRGRLSKDRNRLGTG
                     VGLTDVLHAVQSHQGSVQVSSAPASSTAIRDENHPDYYQQPFITVFTIRLPRRKTKAG
                     "
     gene            complement(10023..10454)
                     /locus_tag="PKL11_01810"
     CDS             complement(10023..10454)
                     /locus_tag="PKL11_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ12362.1"
                     /translation="MSHEITTLCTGCTACVRQCPVDAISGERKQLHQIDAALCIDCGA
                     CGRICPVDAILDANRQTVLHIKPALWEKPVWNYATCTACKICVQACPTGCIAILEPPR
                     NGHGFAPGLPYLARPKDCIACTFCAVDCPFEVIRMASPEFV"
     gene            complement(10464..12425)
                     /locus_tag="PKL11_01815"
     CDS             complement(10464..12425)
                     /locus_tag="PKL11_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNZ12363.1"
                     /translation="MNAVMGKVLWVDLTTGKIEEEQIPDGVYEKVLSGLGLAAYLLYQ
                     RIPPGADPLGPENVLGFVSGLLTGTGSVMTGRWMAAAKSPLTGTWGDANCGGTLAPAI
                     KQCGYDGIFFTGISPTPVYLHVEHGHAELRDASDLWGKDTRETETALWERFEGRPAAV
                     ACIGMAGEKCSLIAGISNDQGRMAARSGLGAVMGAKRLKAIALHGAYRIPVHDAGEVQ
                     RLSDRFNRWIAFQPPFLPGKGMGLLGTLLRVMPLSMRQDGLLYKVILRKWGTTGMNQT
                     SIEMGDSPIQNWRGSSATFKGKTANNLDPDRIIARETVKYHCYSCPLGCGGYCSLKGG
                     GETHKPEYETVLALSGLLMHSDLDQVFEWNERLNRAGMDSISAGGSIAFALECFEQGL
                     LTLEDTGGLALTWGNTAAIDALLDQMIRREGLGALLADGSRAAAQRLGKNASEYAVQA
                     GGQELAMHDGRNDPGFALHAVVEPTPGRHTLGAYLYYEMFQLWKVIKGLPPVRLMFYP
                     KGHKYRNDAEKGVWGAACSNLMAAANGAGLCMFGALVGLNRIPFFQWLNAATGWDKTP
                     EAYMQIGWTIQNLRQAFNLRQGAPLRHAINARATGQPPQTEGANQGRTVPLDGLVRAY
                     YTAMHWDPESGHPDVAAVTALGLPAPTEK"
     gene            complement(12439..13626)
                     /locus_tag="PKL11_01820"
     CDS             complement(12439..13626)
                     /locus_tag="PKL11_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ12364.1"
                     /translation="MMLPPYYEFAFPVKIISGHTALNNLPVELRLANVERPLIITDPG
                     VARAGLITVVLDAFAESGITIGALYDQVPPESSTLVVNELAGLYREKACDCLVAVGGG
                     SVIDTAKGINILVSQGGDDVRHYRGAEVLPGPLRPMFVAPTTSGTGSEATNSAVIADP
                     ETGVKMLFVSENLIPRAAILDTRMTLTLPPKLTAATGMDALTHAMEACLSIQKNPVSD
                     AFAQQAIRLIGANLRVVTANPQEKNGRLALANASLLAGAAFSNSMVGCVHSMGHATGA
                     VAHVPHGIAMNIFLPFGLAFNLPKREAEIAELLLPLAGAEIYAATPAAQRAQKTIDEV
                     HRLKQDLYEISGLPRTLKEAGVSADQLEHIAKLAINDGSAMMNPTELGLEDALKLARE
                     AFG"
     gene            complement(13642..14184)
                     /locus_tag="PKL11_01825"
     CDS             complement(13642..14184)
                     /locus_tag="PKL11_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12365.1"
                     /translation="MISAKIYPDLPPRQKPLQKAVIAAAMFVVGRAFQSLSHSEPLIR
                     HEVSGWPKGLTLMMKLQPNGSSVALQKQPDGTVKYMGAHFDENKADVVIYFKTIEAAF
                     KMFTAQTGIDVAYAQHCMCARGDLSNTVSVVRALGITECYLFPSIISKHLVKRLPAIP
                     FWRKQGLRLKTYLLGIPFGI"
     gene            complement(14181..14765)
                     /locus_tag="PKL11_01830"
     CDS             complement(14181..14765)
                     /locus_tag="PKL11_01830"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ12366.1"
                     /translation="MAARRSSSKEMSNKEHILNAASALFTEQGVEQTSLREIAARAGI
                     STGTLFYYYATKSELIFDITDRHFDRITAQLLGWVRQAHQSMTPAEILGVVFQTIVKD
                     ELRGRLHHYLIEQALTSNGVIRERFIQKYREWREMLQEGLAQVEIPEQDRELVAQIIL
                     ATLDGFVIQSVLGIADIPLNSIAAYLAAHCKEVT"
     gene            complement(14913..15734)
                     /locus_tag="PKL11_01835"
     CDS             complement(14913..15734)
                     /locus_tag="PKL11_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ12367.1"
                     /translation="MKRSRTRLLLYIPLVAMFIFYLVPLYVMLTTGFKSFEEIDLQTM
                     WSLPQNGLHFDNFIEAFTKLAPYFKNSILMVVPAALISSVLGSLNGYVLSKWKFKGAD
                     VIFPLILFGMFIPYQSILIPLVQFMKEINLYGGVSGLILAHVIYGIPITTLTFRNYYA
                     TIPKELLEAARIDGADIMGIFFKIMLPISLPSFVVVLIWQFTSAWNDFIFAVVLTGSQ
                     SWPVTVALNNMAGSQIISWNVQMAGSFLAALPTLLIYIFLGNYFLRGLMAGALKG"
     gene            complement(15731..16630)
                     /locus_tag="PKL11_01840"
     CDS             complement(15731..16630)
                     /locus_tag="PKL11_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ12368.1"
                     /translation="MRRKFDKDRLIGFLLVLPSIVAVAIFIYGFIGWSAKVSLSQWKG
                     LMPNYTWAKPLLSNYLELFKDPRFQVDVRNTALFTVLFVAGALLLGLFLAILLDQDLK
                     GESIFRTVFLFPMAISYIVTGVVWRWLMNPAQGTRMSGFNLLFDKLGLDFLINNWYTT
                     PGWGIAFIAIPAIWQMSGYVMALYLSGLRSIPQELREAARVDGANEWQIYRHIILPLV
                     QPITFSAMIILGHISLKVFDLIIAVAGKQLQLDVPAVYMWQTTFDGGFYSRGAAISIL
                     LLISVAVLVIPYLRYSLKTEESL"
     gene            complement(16697..17965)
                     /locus_tag="PKL11_01845"
     CDS             complement(16697..17965)
                     /locus_tag="PKL11_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12369.1"
                     /translation="MSKKLFVLISALLVAAFVLSACTPKVEEPTSDQVEVFSWWTGGG
                     EAAGLDAMIKIFQAKYPDVEFVNAAVAGGAGTNARAVLATRLQAGNPPDSWQGHAGQE
                     LIGTYVAAGQIEALNFLYEEEGWLDVMPETLIPLISKDGNIYSVPVNIHRANVMWYNP
                     TILAENNIAVPTTMDEWFAALDALKAAGVETPLAMGEQWTAMHLFETVLLGTLGPDAY
                     NGLWDGTTAWDSAEVTKAIDNYIKLLSYTNSDATSLSWQDASQLVINGDAAFNVMGDW
                     AEGYFRELGKAPKTDYGWAPVPGTVGSFQFLSDSFVLAVGAPHRDAAIAWLRVAGSKE
                     GQDAFNPVKGSIPARNDADRSLYGEYLLSAMDDWKANVITGSLTHGVVANDAWKSEID
                     TALGLFLSSKDGAAFQTALVAACMASGPCK"
     gene            complement(18052..>18659)
                     /locus_tag="PKL11_01850"
     CDS             complement(18052..>18659)
                     /locus_tag="PKL11_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ12370.1"
                     /translation="DLAYIKIGIGVGSGLVINHQIYRGESGTAGEIGHTTIEVDGRLC
                     RCGNHGCMEAYVGGPGLLADAQAALQAAHRGADEMPGTVEDLVDTALQGDAVCRQVIQ
                     NAGRYLGTSIANLLNLVNPGLVILGGELVQAGELLMGPIHASIRERVMPIAAQQAAIV
                     AGKLGNDAIAIGAATLVIQNAFEPTQLAKTLNGRQAAQVYS"
     assembly_gap    18660..18759
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18760..19448)
                     /locus_tag="PKL11_01855"
     CDS             complement(<18760..19448)
                     /locus_tag="PKL11_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNZ12371.1"
                     /translation="MPTRPASAAIIRENNLSLVLRLIHASGSISRAEIIRRTGLSATT
                     VSALANVLIESGFVREAGIGESSGGRRPILMEFNYQYRYALGIDIGASHVTTVVMDLS
                     GKVISRETQRCDVINEPELTFEVIKTLTAQALKQAQIEPDHLLGVGFTVPTPLGGEAL
                     DRLVTYYMPVWEGILPKPKIRAFFPGLPVYLENDANAGAIGEKWWGSGQEFADLAYIK
                     IGIGVGSGLVI"
     gene            19603..21156
                     /locus_tag="PKL11_01860"
     CDS             19603..21156
                     /locus_tag="PKL11_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HNZ12372.1"
                     /translation="MAHPNFCSAAYTDADKNSPGGIHSMRVLMLYPEFPDTFWSFKHA
                     LRFVRKAASSPPLGLMTIAALLPSTWEKRLVDLNVARLRDRDIAWADVVMVSAMIVQR
                     QSTIEVIERCKQAGKKVIAGGPLFLAEWESFPTVDHFILNEGEITLPLFLSDLARGCP
                     QRVYQTDEFADMAQTPTPLWELIDLSKYDSLSIQYSRGCPFQCDFCNITAMLGRRPRT
                     KSAAQIIAELDKMYELGWRRNIFFVDDNFISNRRQLKEEILPALIAWRKGKASCNFLT
                     EASINLADDEELMDLMTAAGFVSVFVGIETPDEGSLTECNKGQNIHRDLMDSVHCLQR
                     HGLQVMGGFIVGFDNDTETTFDRQIDFIQKSGVVTAMVGMLQAIPGTILHDRLTAEGR
                     IAHQATGDNADGTTNIIPRMGIERLQAGYRRLMAEIYSPRRFYERVKTCLELYKPSNA
                     PVHLQFDEIYAFLRSIWKLGIIGEERREYWKLFFWTLFRHPRKLALAITFTIYGFHFR
                     QVNALNGIS"
     gene            21166..22026
                     /locus_tag="PKL11_01865"
     CDS             21166..22026
                     /locus_tag="PKL11_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ12373.1"
                     /translation="MPPYSIGLITDSTCDIPAELRQQYQIGIVPLTVVWGGKQMLDGV
                     ELSADAFYERLVKDPEIPTTSQPSPKQFLEAFKAQQEAGAREIVVITISSAMSGTIES
                     ARRAAEMMDIPIHIYDSRSNSMSLGWQVLAAARAREAGGDAAAMITAANQARQRMVYI
                     IVLDTMDYLVKGGRIGGAVRFLGSLLKIKPQISVNHETGKVEAGIPARSRDSAINGLV
                     RDFFRRLNPQGKLHLAVLHNAAPADATALAEQVRRDHAPAELVMSIVSPCLGVHTGPR
                     AIALCGYTED"
     gene            22213..22572
                     /locus_tag="PKL11_01870"
     CDS             22213..22572
                     /locus_tag="PKL11_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNZ12374.1"
                     /translation="MNIYVGNLPFSATEDEIRALFADYGQVTSAAVIKDKFTGQSRGF
                     AFVEMTSDTEAQKAIAELNGKDFKGRALVINPARPREERSDRGPRPGGFGGGGDRRGG
                     GPGRSGGDRRGGGRTGW"
     gene            22988..24523
                     /locus_tag="PKL11_01875"
     CDS             22988..24523
                     /locus_tag="PKL11_01875"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNZ12375.1"
                     /translation="MIFKDYLLIVWRRRWILIFTILVTLSVVAVGTYLTPPTYAASAI
                     LRVATASSGSVSYTDYVYADRLVNTYVHISTSRPVLDELAVRLGRTEAPEVQVQIVTN
                     TELLSISVEDSDPQLAATAANTLASILIEQSQSLYTGGGRDPKDILADQLTQIEAELT
                     AARAAYDELVRNSPSDSEAIAAASSAIDLKQNTYATLLEQYEEARLSDALRANTISIL
                     ESAIPPEKPIRPNVLINFGLGFILALLAGLGLVFVFSHFDTTLYTTSQIERLVGQSAL
                     AHIPVTHPQQVLLTGSDGRSPYGQSYRRLRSTFLNQLQTLACQTVMITSPEPEEGKST
                     IAVNLAVTLAQAGRRVILVDFDLARSQVAQILNLPNELGLANVLTGQTELNSALQSAA
                     CPGLRVLTSGTSASESGLYLNSSLVGDLVNQLAAQSDIVLFDSPAILLISDSTDLAAE
                     VDGVLMVVRRGATRQPALEEARSLLDKLNTPIIGYIVNRAEANHATAYYTRPRQTMGR
                     RRL"
     gene            24570..25301
                     /locus_tag="PKL11_01880"
     CDS             24570..25301
                     /locus_tag="PKL11_01880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNZ12376.1"
                     /translation="MQTDKLSEIESQPVPRSSSRSRQAYYLMKRCVDVLLSFIVLVLL
                     LPILLVLALVIRLDSPGPVIYHQTRVGARRVGGQWQRADFTFFKLRTMRQNTDASLHQ
                     AYVHALIQNNTAEMAALQGAEMGNVNKLVHDPRITRIGHFLRKYSIDELPQFFNVLRG
                     DMSLVGPRPALPYEISDYQPWMLERLQAAPGITGLQQITARCTADFASQVRLDIEYIH
                     RQTLWLDFVILFKTPFAVLRAEGAC"
     gene            25321..26337
                     /locus_tag="PKL11_01885"
     CDS             25321..26337
                     /locus_tag="PKL11_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ12377.1"
                     /translation="MDKLRIGVIGYGYWGPNLVRNFVEIPGAEMIAVSDLRIERLAQA
                     KSIYPSLQTTTNYFDLFDMDLAAVVVATPPTTHHNIARDCLEHGLNVLVEKPLTLTSD
                     DALALCNQAKASSLTLMVGHTFEYNAAVHALRKLMSDGDIGEVHYIDTARLNLGLFQR
                     GLNVLWDLAPHDISILLHLLQQPPVSVSAHGMACVFKDIHDVAYITLLFPNDVLAHVH
                     VSWLDPCKVRRVTVVGSKKMVVYNDIEAQEKLRIYDKGVDGPGLSESQDDFRCSYRYG
                     DIIIPNIKLGEPLRAECQHFVDCIQKKEKPCTCGMEGYQVVRILEAAQRSMSNSGHQE
                     ILEW"
     gene            26331..26819
                     /locus_tag="PKL11_01890"
     CDS             26331..26819
                     /locus_tag="PKL11_01890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNZ12378.1"
                     /translation="MVTPEFARIAPDVRLGANVRIYAFVNLYGCQIGDDSRIGTFVEI
                     QKGVSVGQRVKISSHTFICEGVTIEDDVFIGHGVMFINDKYPHASTAAGQPQTEADWI
                     CTPTRVERGASIGSNATILCGVTIGAGAMVGAGSVVTHAVPPGAVVAGVPARILRKPS
                     QS"
     gene            26845..27951
                     /locus_tag="PKL11_01895"
     CDS             26845..27951
                     /locus_tag="PKL11_01895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ12379.1"
                     /translation="MSPIQFVDLKKQYAPLKEEILAGVARILDGMQLFLGENVRALET
                     EFAAFCGASYGIGVSDGTTALHILLRAMDIGAGDEVITVSHSFIATAEAACLACARPV
                     FVDIDPQTGLMDVSQIEAKITPRTRAILPVHLYGQMVDMEPLLEIARRHNLRVIEDAC
                     QAHGSEYRGRRAGSLGDGTAFSFYYSKNLGAYGEGGFITTNDPEIARRCRMIRDHGSE
                     VRYQHALIGLNGRLDEIQALVCRVKLPYLADWNTLRRQHAARYAELLADTPVKMMVER
                     PECRSVYHLFVVRAPRRDELQAYLKAQGIFTGIHYPVPIHLQPAMAFLGYRPGDLPAT
                     EQLVGEILSLPMFAELTEDDLSTVSAAIHAFYQG"
     gene            27978..29987
                     /locus_tag="PKL11_01900"
     CDS             27978..29987
                     /locus_tag="PKL11_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12380.1"
                     /translation="MRRAELLAALVVGLLLGAVIVAAALLQNKSPRPVTPRSATPLQS
                     ASAVIRTPTPTPIPSAVAPAATLTPSGPPLLEADPPGTDAPDVLLLFNPARASSFGTN
                     FCRLAEFYGLVCTQFNVTQRDTLTAADLLGPQGLPYPLIALDATLLDNRSPLLSSGDL
                     EVLMEAVNQQGVSVLVGKLDDLQNADLVSALTAGAVAGVTRPEDTTKDWQVSRLAPEI
                     TLELTGQNIITSQAATPDTFALNIVDPLPVTVLISSTDNSGASYPIFLRWQPSDQPAA
                     GAVYLDSGETGQSLDFIALREVYYSAASFSQIIPTMLTLRHVIGEETWHAPQDFANLT
                     IDEGALLEPYQDLNYTALLELMRRNSYHTTLALIPQNWQLSESDVVALFLTAPDYFSL
                     AQYGNTGAGYEFYRYATPAPDATDVPLLPARPLDDQQRSILEGLARMQLHENLTRVPF
                     DRIMIFPGGISPVQTLEFLKANNYLATVNTQDLPLDAVRPTNWDYGMTPAITDFAGFP
                     NLIRRMPTENQAYQPYTLASILDLLVDKPALFYTYPYGSGLFSAGMDAFDSIAGKMNA
                     LEGEVSWRSLGEIAKRLCRIKRDDDGTVSVQMYTRWLILANPDAADTVFHITKSEPLN
                     VPIVRLTVNGYAFPYTIQDGSLTLNLIIPAQSSAEIIIEYSQPAE"
     gene            29962..31422
                     /locus_tag="PKL11_01905"
     CDS             29962..31422
                     /locus_tag="PKL11_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNZ12381.1"
                     /translation="MNIPNRLNNWIWRTTRLSPKVWLAQIGLLVLASILIGALAVLQD
                     RLPQKVGYLVVVAAAGACTLMMIGNLKKVLLAAILIDIPLRWDVYLGARVVNSPVGMR
                     EGWMIGLTTLALAGLFGVYLLQELARPHSHPRMRFVDNLPLFLYMVVVCLSTFVAADT
                     RGALFQIFFLAQMYLLYVYIVSVTKTAQDVWFIMLFLLAGLALEGLIVLAQRFLGLQI
                     NLLGIVTVNSYGRMAGTMGSPNVAAGFFSLLLAPALGLAFTRIPTLARGGALIAFGLG
                     GLGLLFTVSRGGWMAFAISLMFLGLMLWLRGKISGGVVILVMFVAVIGLVLFGGVLFD
                     KLVGMRTNAAMARIPLMKLAWEMISDRPFLGVGANNYVMNMKQYMIPELTRGFLYVVH
                     NKYLLVWAETGLGGILTYLGFLGLTLWRGWQCWNRDHPLFSPLALGLIAAIVGQMAHM
                     MVEIFDARSQVQSLWLVAALVSALELMTRGGSHESA"
     gene            31409..>31602
                     /locus_tag="PKL11_01910"
     CDS             31409..>31602
                     /locus_tag="PKL11_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12382.1"
                     /translation="MNPPDAAPSVVHSRTRAARLIGNTASLVTNDLVNRAITFVLYAL
                     IARYLGAYEFGQMSITLIIF"
     assembly_gap    31603..31702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31703..33039
                     /locus_tag="PKL11_01915"
     CDS             <31703..33039
                     /locus_tag="PKL11_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ12383.1"
                     /translation="YLGAYEFGQMSITLIIFYLLQSLAPMGLKMLLVRETARNPQNSG
                     AYLVQGSLVSLAASILAIWLVFGFLSIMNYAEDTTWIIMLISFGLIPYSLSAVCEAIF
                     QAHEQIRWITLANVPVSLLRGLAAFWMLSAGLDLRWIGILFLVTFTITWLLEWILLTR
                     HIAPPNWSGPPALFVQIARMAVPFIGLQGIIAITNSILPLFLSKSSGEVQVGLFNAAN
                     QIMAPVLLVSQSVVVSLFPRLCQKYDTGQQALRQTTDRLTELLFAVTVPAVIGLIFYA
                     RFVLLLIYDKEIFAISALLLQIMACSLLFRAITSVLGRVLMASGRERTLLRILIIETI
                     ICLALAFTLIPRWGVLGAALAGLIVTFFDLILHLPPIFRLLGGLSLGRSAWKAINASL
                     VLIIWLVFAMGVQLNPWLGILVSLFLYPVVWLILALLESGGWRRLLERYALR"
     gene            33042..34232
                     /locus_tag="PKL11_01920"
     CDS             33042..34232
                     /locus_tag="PKL11_01920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ12384.1"
                     /translation="MRVLYLTLPALMDASLSFARAIAAHVELHMLLQVTPGGWRTSLF
                     DVNPVSPSSDLLPVEPLLSGYPAALSSYWRASASFQAAVFGGSSALHPASWAAARSVL
                     RFARQLQPDIIHLETTLGRMVGALPLLRRLAPLVITVHDPSPHSGESPLKKVLIRALT
                     YARADHFILHNRTQSAQFCQTYHVPAGRVSVTPLGVYNLYRLFSQTTPLSDPHTILFF
                     GRLSPYKGLETLLQAAPQACLQIPRLRFVIAGRPNPDYRLPSLPALPNGGHFEFIPDY
                     IPNARLASLFQQSGLVVCPYTDATQSGVVLTAYAFGRPVLASRVGGLPEYIDEGRTGW
                     LVPPGDVSALAAALVQAVTALRSDGDAYRQAIAARSAADLNWETIAAQTLDIYRQTRQ
                     EWKS"
     gene            34229..35410
                     /locus_tag="PKL11_01925"
     CDS             34229..35410
                     /locus_tag="PKL11_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ12385.1"
                     /translation="MKPVISVFFDGLKPESLSVMPFLNALPHQRRLETVLGYSVTCHA
                     SMYTGVYPDRHGLWFLWQHSPQTSPFRWITRIPLLTHLDCLPVWMLVHRLTARRTHLT
                     AYWGIPRLYNLPLRYWPNIDVAEKRYWDEDGYLEGCPTIFERLRQANLSYEVVGMVPH
                     VIDTSRLVAEHRFTEVRPWHYLFIGDVDALTHHATQTAPEVLDKLRQFDQIVADRCAE
                     MERLAGGYELVVWSDHGHVPLQQKIDLYALFRQSGDRLSRYMHIIDTNFARFWFRDDR
                     ERQQVTAVLEKIPNGFVLSPADMQREHVTMPDNRYGDLIFYLDAGSAFSRTIFGFGLR
                     QKSIHGYLPSHPDCDGVFLSTAPIAPAVERVRLVDIQPSLLALLGQPIPSGLDGRVIW
                     S"
     gene            35413..>36370
                     /locus_tag="PKL11_01930"
     CDS             35413..>36370
                     /locus_tag="PKL11_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12386.1"
                     /translation="MGRYTLQTDPRVDQMVSEHLEIIRAAVLRAINPQALLLAGSFGR
                     GEGSVSVSEAGLTLLSDYEVCLISPSPAARLAVDRILQDLDGKISASVSLFWNTPARI
                     RNNRARNLSFGRPRPTIGMYELKAGSQLLYGDFDLTYNQIDPAAIPPSEGVRLIVNRM
                     MEVIDVWRGGGGLGLPLAKLTLACGDALLLISGFYHYSYSRRALRFDAAFPHFADRMD
                     PHFLTLYRRAAERKLNPGPNAPFDITPEEVTSVSRACQQTLAILLDKLDAAPCQLAVR
                     CAAAVPILYCTGVAPALDPLYERLILALRARRAGQSIYWRGLP"
     assembly_gap    36371..36470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36471..36731
                     /locus_tag="PKL11_01935"
     CDS             <36471..36731
                     /locus_tag="PKL11_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12387.1"
                     /translation="LALRARRAGQSIYWRGLPHRRPMLSPFQALYAAIPALFWGLPLT
                     NQPNPDLLNLAANWSRWILPSAAAENPLELTSALLRAWHTLG"
     gene            36739..37872
                     /locus_tag="PKL11_01940"
     CDS             36739..37872
                     /locus_tag="PKL11_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ12388.1"
                     /translation="MNRPVILHLLNSLSDSSITRIVERIIRQQGAAAVDWRVAALGGS
                     SGLVQLLTSLGAQTLDFSARPSWEIVQAYLRQNRVDIIHTHTPHTIITAWRAISGLPK
                     PHRPVHLATKHLLTRPADRRWGAFYALADWLELYLPDHLVAVSQTMARQITRLPGISS
                     HRVTAIPNGIPVDLYAAADNRSAARLELGIPADALVFGYAGRLDQVKRLDILLSAFVD
                     VLAQVPHAHLLILGQGSMQSTWQAEAQTLGLERAICWAGFRHDIPRMLSAMDIYVQCS
                     VNEGLSLSILEAMSARLPVIATRVGAADEVLAHGDTGWLIPPASSAALAQAMLRLASD
                     ADLCQRLAAAAYRWVGARYSVKSMTAAYFQVYTRLLEGRADHA"
BASE COUNT         7635 a        11848 c         9569 g         8521 t
ORIGIN      
        1 tgatggaaaa actggaccgc cagcgcaagc tggaatactg gatcaaacgc tatggcggct
       61 gggctatcat ctttttagcc ttcattccca acccgctgtt tgatgctgcc ggcatgattg
      121 ctggcgcgct caagatgccc ctctggcgct tcctgatcta ctgctggatt ggtaaatgcc
      181 tgaaaatgct ggcatttgcg ctgggcggcg ccgcactcat tgacctgatc ccctggcttt
      241 agcccattat ggctttggcg ttatccgcct gagatttagc ttttcccgcc gcagctcggt
      301 cagttttcgg cgcccttcgg cttgaatttg ccgccagcca tccagcactt gttgaacctc
      361 agcctcaagc tcagggtatt cgccgtatcg aatttgcgta tagacctgtg taatctggtc
      421 tgcctgttct tgatgctccg ggaacagccg ttgcagcgct ggtaaaaatt ccagcggtgt
      481 ggttgctggg ggtcgggggt gttccagtgc agcacataac cccattaatt gtccgtaagc
      541 ccttctgacc cgcgctgccg ccagcacctg tgccgcgctg cggggcttcc ctcggttggc
      601 ttctggcagg gttgggatcc tctgaacgcg gctgacgttg cgccgctcgt cttcacccgc
      661 ctcggctcgc ctgatccgcg ccgcattcaa tcgccgaata atcaagatga tggcaatggc
      721 caggataatc cccaccaccc acagccgggc tgtttcttcc cagttgataa tcttctccgc
      781 tgcctgttcc atcaagtcaa gcattgtcgc ggccgtctgc tgaacggatt gttgcaatgc
      841 ttcaatggcc ttgcgattgt attccggctt gatgacgcgg attagcagtt gaatcagccc
      901 aaccaacggc accgagatca acagccctgc cagcaaaaca atggccagca gtcccgtaaa
      961 gatgcccatc aagagcttgc ctatgatttc tccaaaaacc tgccccgtca gcattcccaa
     1021 tcccaccgcc gccgcggcca acccggcaac gctcaaccag cttcgccacc gaaatggtgg
     1081 cacccgcccg ccccggctga ctgacagatc cgcaatgcgg gtggcgctca ggctcaataa
     1141 ccccatcaat aaaaacaggc caacagggat gatggccagg ctgcttttat ctgcggacgt
     1201 gattaagaag tacaacccta acagcaaaat cccggctcga aaactgccct gagttcccca
     1261 actatccagc gccgaggttg ccagcgtcac cccgcgcagc gccagcagcg tgataaccag
     1321 cacatgcagc gcttctgcca gcgccagccc gctgaagatc tcgccgcgcg gctgccagcg
     1381 gtaaaccaga gcatttaaag tcaagatttg tatcagcacc agccagacaa aaaacgcgcc
     1441 gatccgaata ctgcgcttaa tcctcaagaa attaaagact cgggccagaa aatgactgcc
     1501 cagttccagg gcaaataagg ctgcaattgc ctcccaccag ggggtgcggc ctgcctgagt
     1561 gtactgatac cagacactga cccaggctgt atccatcccc atcagcgtca aggtgctgat
     1621 ctctgcccac agcgtggctg aatggcgttg cgggctagct ggctgcatgt tcctcatcct
     1681 tgaccacaaa aggcatatga atggactgca cccccggaat ctgcggcggt gcttcttcgt
     1741 ctaacgatat caccgtggtg tgggcccggt agcgcttcaa ttgcaccagc gtttcggcca
     1801 gggtcggggt gacgaaggct gtcaccacca tcaacgtagc ccccagcggg attcgcggcg
     1861 ccgcgcgcag tagataattc tcaaatggaa tcgttgtgta ggccgtcaac ccggccaggg
     1921 tttccagcaa agtgcccaac tggctggctg cgcgtcccgg cgaaaccaaa aacggctggt
     1981 cggctctttt gagattgcca ttcgagagca gccccaccga atagccatcc tgcaccgcgt
     2041 gatagatcat cgtcgcgctc acgctgataa gctgctccag catggggggc acaacccctt
     2101 cccagatatg atgggtggtt gctacattaa cacagaccac catcactcgc gctgtcaccg
     2161 gttgaaaaac ccgggcctgc aacgccccgg ttcgggcagt cgccggccag tgaatccgcc
     2221 gaaaatcatc tccgggttgg taatcccgca cacccattgg ctgtgtaggg tcttcaaata
     2281 accgccgccg cgcaccccga tccccaaagg gatcatctgc gttcagtcgc agtgttttaa
     2341 gcggcagcat ctgcgggaac accaccagcc ggtccgattg ttcttcctct cgctcggtct
     2401 caaacagccc aaacggatcg cctgattcta atttgaccgg gcccagtgga tagaccccgc
     2461 gctgttcaaa ttgaaacgga aaccgccggg tcacgcgctg ctgccagcgc aaactgtatt
     2521 gattgatgac ccagccgcgc tctggcaggt gggatggcgc tatcagggtc gcgtcctgtg
     2581 gcggtgcgcc agtgggccag gagtcgctcg tccgcatcca gatgaccggc agccgcttgt
     2641 gattggcaac atcgatctgc gcctcgctgg attcgcccgg aaaaccgcgc cggtagctga
     2701 tccgccgctg gtagcttacc tgccgcaaag catggcgctg ccagagccgc gccagcccca
     2761 caatcacccc cagggtcgtg gcaaatgcca ccagttccgg cagcccgaaa aaagctcccg
     2821 caatgataaa cagtaccagc agcgcaatcc agcggtcgat gggcattatc cctctactgg
     2881 aaccggcacc gccgagacga tatcttcaat cagctctacc gcactccggc cgcgcaattg
     2941 tgcctgtggc gcaatcatta accggtgcgc aaacacgtcc aacgccatgg ctttaacgtc
     3001 atctggcagc acaaacgcac gcccttgcag cgccgcccag gcctgcgccg cctgaaagag
     3061 tgccatcgtg gccctggggc tggcccccag ttgaatatcc gcgttctggc gggtggcgcg
     3121 ggcaatccgc accacataat ctcgcactga atcttccacc cgaatggcac gccgttcctg
     3181 ctgcagcgcc tgaatttctt ccggcgtcgt cacagcttcc agttcaacca gtggatcgct
     3241 caatcggaac cgcgccagaa tatcattttc ttctgtctgt gttgggtatc caatttgcac
     3301 ccgcagttga aaccggtcaa tctgagcctc cggcagcgga aatgttcctt ccaattccac
     3361 cgggttttgc gtagccatca ccaggaaagg ccggagcagg gggcgggtca caccatccac
     3421 cgtcacctgg cgctcctgca ttgcctccag cagcgccgac tgtgttcgcg gcgttgcccg
     3481 gttgatttca tcagccagga tcacctgggc cataatcggt cccggacgaa attcaaattc
     3541 ctgctgcttt tggttaaacc aactcaaccc ggtcacatca gaaggcagta gatctggcgt
     3601 gaactggatc cgccgaaagg cgcaccctaa tgaagccgcc agcgcccgtg ccagcgttgt
     3661 cttgcctgtt ccgggcacat cctccagcag cacatgcccc tcgcagagga tcgccgccac
     3721 caaacgccga atcacgtcgt ctttgcccac aatcactttt tgcacatttt gaacaatttt
     3781 atgcgccgca gcttgaatca tactcatcct ccaatatcga tgcttatgcc ggtctgtacc
     3841 ggacatcaat catgtcacca acttttgcct tcaggcgttg cgctgcattc ccattgacca
     3901 cgcacaccgc cagatatccc gaactgtcga tcattgccac caggctgccc ggtccacggt
     3961 ctccaaaagt cagcaccagt ggaatctctg tatcgccaaa atggatcgtg cagccctgcc
     4021 cttccagtgc ttcaatctgc agcccggttg ccagattgcc aaaatggtca acatgtaaaa
     4081 ttggcgcttg ccagccacca acggttgccg tgggctgggg tatggtcaac ctgactggct
     4141 cgcgcatcac cgttcctaat ttttccagcg ccgccccatt cacaatatgg gcggcaaccg
     4201 gcgcaaagat gtctcgccca tgaaagctcc ggcttgggtt gggcagccag aattccgcct
     4261 gatcaagttg gacgatgata accggctcgc ctgccgtctc agcctgctcc agggaaatgg
     4321 tcagcagacc attgtctggc cccacaaaat accgcgtccc aatccgggca gcaatcgccc
     4381 gccgttctgt tcccacgccc gggtccacaa cccccacaaa gacagtccct tcagggtaat
     4441 aattccaggc tcgtgccagc gtcagcgcgc ccgctagaat atcctgaggt tcgatctgat
     4501 gggtcaaatc ggcaatctgc gcattaggcg caatggacca gatcacgcct ttcataatgc
     4561 ccggataacc atcccggtcg ccaaaatcag tcattaatgc aatgatcttc atatatcctt
     4621 atcactgtca gcggggatga agcaattgta acccatcgga gatgaaaatc agattaacaa
     4681 atcgttcccc ctcgcttata aatttctgat attattagac attggcagta taataagccc
     4741 gacttggtaa agtcacatca ttattatccc ttaggagcat acctctatgc ctgaaaaaat
     4801 cccctttcgt gagcgtttcc aatattggct cgataaccag ttctcgcgcg gcactggcgc
     4861 cctgatcctc tggctgggca tcctttcgct ggcggtgatt gtggtcgctg gcggcattat
     4921 tgccttactg gggattgccc cggctggcgg cgagcgtctc agcttcctgg aggcggcctg
     4981 gcagagcctg atgcgcaccc tggatgccgg taccatgggc ggtgatgaag gcctgggctt
     5041 cagagttatc atgttcctgg tcaccctggg cggcattttt gtcatcagca ccctcattgg
     5101 tgtgctgacc agcgccgtgg aaggtaagct ggaagatctt cgcaaaggcc gctcacgcgt
     5161 tcttgaaacc aaccagattg tcatcctggg ttggtccgaa caaatcttta cgattgttac
     5221 tgaactgatc gccgcaaatg aaaatgtcga aaagccctgc atcgtcatcc tgggcaaaaa
     5281 agacaaagtc gaaatggaag atgaaatcgc ttccaagatt gaagaccgca aaaacatgcg
     5341 catcatctgc cgcagcggcg acccgatgga tgtagcagac ctgaatctgg tcagcctcaa
     5401 tcaggcgcgg gccattgttg tgctctctcc cgaagggaat gatcccgact ctgaggtcat
     5461 caaggctgtc ctcgcggtgg tcaaccaccc caaccgtgac cccgaaaaaa ccttccacat
     5521 tgtggctgaa atttacgaac ccgaaaacat ggatgccgct ctggtggttg gccgcggcca
     5581 ggtggaatgg gtgctggtcg gcgactttgt cgcccgagtg gttgcccaga cctgccgtca
     5641 atctggtctg tcggttgtct acaccgagct gcttgacttc ggcggcgatg aaatctacat
     5701 caaacccgaa cctcgcctga cgggcaaaac ctttggcgaa gcgctgctgg cctaccgcac
     5761 caacaccgtc ctcggtattc gcaaacccaa caccatgccc tgcctgaatg cccccatgga
     5821 tcaggtcatt gaggctggcg atgagctcgt cgtgattgct gaagatgatg acaaaatttt
     5881 cctgactctc gatcagcctg ttcaaattca ggaagcccag atcgtctcgg ggcacattgc
     5941 ccccaaacag cccgaagcca cccttctgct gggctggaat cgccgcggcg ccaccattat
     6001 tcgtgagctt gataactatg ttgcccccga ctccgaagtc accatcgtgg cagatgtgga
     6061 taccgttggc actgaccttg aaatttgctg ctctgaactg acgaaccaga aaatcacctt
     6121 tcaacaggcc gataccacca accgccgcgt tctcgaggga ctcaacctcc gccacttcaa
     6181 ccacatcatt ctgttgtgct actccgactc catggatgta cagcgcgccg atgcacacac
     6241 attggtcacg ctcctccacc tgcgtgatat cgccgaccgc gacggcacca aattcaccat
     6301 cgtcagcgaa atgctggaca ttcgcaaccg caaccttgcc gatgtcaccc gcgctgatga
     6361 cttcatcgtc agcgataagc tcgtcagcct gatgatggcc cagatcactg agaataaata
     6421 cctcaacggc gtctttaccg atatgttcga ccccgaaggc tccgagatct acctcaaacc
     6481 cgcggccgag tatgtccagc ttggcaaacc ggtcaacttc tacaccatcg tagaaaccgc
     6541 ccgccgccgc aaccaggtcg ccattggcta ccgcctccag gccaactcgc aggatgctgc
     6601 caacggctac ggcgtcaaga ttaaccccga caaagccgaa atggtcacct tcaccgacca
     6661 tgatcgcatt gttgttattt cagaagagta acactgagcg aatgccaagg ctcaaatgat
     6721 tgtaacgaaa cgagcgggaa ttttcccgcc cgtttgcgtt acatctatca atattcagac
     6781 cacatcttag agattttgtc cgccacctca aaccgcagtg tctcacggtg atgctcgata
     6841 gcacgttcca ccagttcttt caaattgatt ttcccccctg ccttcaaatc attcaaatct
     6901 cggttggcaa tgctctgtgc actttcacca tgagcccaga atgcccccag cagccccagg
     6961 taatcgtaga ggatttcttg cagcttggtg tcttccaatc cattttcata gactgctgat
     7021 ccaataatat tgagtgcctg catcgccacc agaataaccc cctcctgcag cgccaccgaa
     7081 gcaatctcac tcaacccctg gctggctgaa ggcgacagtg gtctgctgct tgattcaagc
     7141 acattgatgc aggcaatggt aacatccggt gttggaaatt tgattgcttc ttgtcctaaa
     7201 tcgctcagag ccttcaggct ccaccttaag tcagcttgag ataaccccgg acgctcgcga
     7261 tgcttctgga taatctggtt caaaatctcg attgccttgt gaaaaatctc tggtgaactg
     7321 ccttgccgtc cctctaaaat gattccgcgc agcgcatcaa tggctgcctc catttcattc
     7381 ccggcataat tggttcggct taatcggtaa ctcgcaattt tgccaacggc ctcaattgcc
     7441 cagagcttct cgccgcttga tttgctttct ctgcccagca gccgaatatt ttcaaaatag
     7501 tcgttagaca tggcctcggt tttttggtga gcgccaatgg cctgatccgc ctgcttttta
     7561 accacatcgc gtttgctggc ataggataga attttcacgc acacaaagat ggttagtccg
     7621 atgagcagca ccagcaagcc gacctcagcc catttgcaag gcgtaggatt gctgaacacg
     7681 aaaacaccca gggtcgatac tgcccccacc actgccggcc aatatagatt cgcaataaac
     7741 ttccacttgt cagacttgtt cttgagcaga atatggcgca catcgtttgg cgtaaattgc
     7801 agcaccaacg ccggagcgcc cgccataaac agcaacagcc caataaatgc atccaggttg
     7861 ttcatgtgcc cctcttgtcc tgctcttctt tgcagttcat cacccgccca acaagctgca
     7921 ccagaatatc atccgggtaa ctggcatctt tgggttgata gaccttaacc cccatcttga
     7981 atatgcggtc catgccagag ggttcttctg ccgaaaacac cgcaatttgc tgcggttgaa
     8041 tccaatttgg gcggacagag aattgctctg catgtccatt catttgcccc agcatccatt
     8101 caatcagata ttgccccagc cgggctttac tggtatcgtt gccctgatct tgatataaca
     8161 tcttccaggc tggatcttcg cctggtggaa tccgcatatc aacaatgatg acgtcgtaat
     8221 gctctgttgt acggattaac cgcatggcct cggtagcatt ctctgccagt gataaatggt
     8281 aaatccgccg atataacaac ggccccatca actcattcaa ctgatagcga gcctcatctt
     8341 caatccagag cacacgaatt cggttgggta acataatcta tcctgctttg gttttccggc
     8401 ggggcagccg gatggtgaaa acggtaataa agggttgttg ataataatcc gggtgattct
     8461 catcccgaat agccgtcgaa gatgccgggg cactgctgac ctgaacactg ccctggtgag
     8521 attgtaccgc atggagcaca tccgtcaatc ccacacccgt ccccagccgg ttgcggtcct
     8581 tagacagccg cccccggtat cccaggttga aaatcaaatt attctcaatc tcatctttgg
     8641 ggatgggcac cccccagttt tccacactga gcgacacttc gctgtcattg gcgcgaattt
     8701 caatcgagac ccacggcggc ttggtgccgc cagtgcggct ccaactgtac ttgatggcat
     8761 tatgcaccag attggcaact gcccgctgca gctcgcgctc ctgcaccgca atctccgccc
     8821 cgcctgaatg gtcccggatc acaatctcaa tcccccggct gctggcaaaa tcagccagcg
     8881 acttgacggc gttatccaca atcgagcgca tagaaacaga cacccgttcc tcaccagacc
     8941 gcagatcacc agttgcaaaa tctctcaacg ccctcagcga atgttccatc tggatggagg
     9001 ccacataggc ccgggttgcg gttaacaggg ccgccgcccg ttgtaagttg agcgccgccc
     9061 gctgcacctc gcgcacctgc tcctttgcca gccccggccg catctctcct aaaatagcag
     9121 ttacatctgc cgccagccgc cgcagaaaag aggggcgggc ttcagtcacc ggcacaacct
     9181 gttgataatc attcaagtcg cccgccagct gctccagata ttcccagcgc agttggggca
     9241 gtgcctgcaa gcgccgctcg gccggattat tggcaatcaa cccccggatg gctccgccca
     9301 gcttctgcgc ctgtccgtcc agcagcgcgg tttcctgtac gggcgataac tcaccctttg
     9361 gattgggtat ctccagctcc gtcgcaatca gcgccagcac ggggtcaaag gccaaacgca
     9421 ccatcgtcag cgtactcgaa ttggcgtgca gtacccgccc aatatccgta gtcaggtcct
     9481 caattcgctg ctgcagcttc tgttcctgct gcttgctttc cacctctgcc gtgcgatcca
     9541 ccagtgtccc atagtaaccg accactgccc cgatctgcgg atccagcagc gcccggcagg
     9601 tgttttcggc cacccgcaga ccgcccctgg tgcaaaaccg gatcagcgcc ccctgcagat
     9661 accgcccctg gcgtgatctt tcctcagctt gctccagcag catggtcctc tgggccggat
     9721 tttcgtaaaa ttctgcgatg gtggtgttaa tctccccctc caacggcatg cccagcaaat
     9781 accgcacccc gcccgatgca atcaaaaacc gcccatccgg cgccaccagg taaaacccaa
     9841 taggcagctc gctcggatga acttgccgaa aaagtttcag gacttccgca tcggagattg
     9901 tcatggggaa accccttgct gtcagaatga attttattat acggcaaaac aaccgaactc
     9961 acgtcaattt agctgcgtga gttcgattgt ttcagacgct tagccgctta aatttaccct
    10021 gttcagacga actctgggct tgccatgcga ataacttcaa acgggcagtc caccgcacag
    10081 aatgtacagg caatgcagtc tttaggccgc gccagatatg gcagccctgg cgcaaaccca
    10141 tgcccgttgc ggggcggctc cagaatggca atgcagcccg tagggcaggc ctgcacacag
    10201 atcttgcagg cggtgcaggt cgcataattc cagaccggct tttcccacag cgccggcttg
    10261 atgtgcagca ccgtctgccg gttggcatcc aggatggcat ccaccggaca gatgcgcccg
    10321 caggcgccgc agtcaatgca caaggccgca tcaatttgat gtaactgctt tcgttcgccc
    10381 gagatggcat ctaccgggca ctggcgcaca caggctgtgc acccggtaca cagagtggtg
    10441 atttcatgac tcatcgctca cactcacttt tccgttggcg ccggcagccc cagcgccgtg
    10501 actgccgcca catcgggatg cccgctttcc gggtcccagt gcatggcggt gtaataggcc
    10561 cgcaccaaac catccagcgg caccgtgcgc ccctgattgg cgccctcggt ctgcggcggt
    10621 tggccggttg cccgggcatt aatcgcatgc ctcagggggg cgccctggcg caggttgaag
    10681 gcctgccgca agttctgaat ggtccagcca atctgcatat aggcttctgg ggtcttatcc
    10741 cagccggttg ccgcgttcag ccactggaaa aacgggatcc ggttcaaccc taccagtgcc
    10801 ccaaacatgc acaaccccgc gccgttggca gcagccatca aattactgca ggccgctccc
    10861 cagacgccct tctcggcatc attgcggtat ttgtgcccct tgggatagaa catcagccgg
    10921 actggcggca gccctttgat gaccttccag agctggaaca tttcatagta aagataggcc
    10981 cccagcgtat gccgcccggg ggtgggctca accaccgcat gcagggcaaa cccggggtca
    11041 ttacgcccat cgtgcatggc aagctcttgc ccgccggcct gcacggcata ctcactggcg
    11101 ttcttcccca gcctttgagc cgccgcccgg ctgccatctg ccagcagcgc tcccaacccc
    11161 tcgcgccgga tcatctggtc aagcagtgca tcaatggctg ccgtgttgcc ccaggtcaac
    11221 gccaatcctc ccgtgtcttc cagagtcagc aggccctgtt caaaacattc cagcgcaaac
    11281 gcaatcgagc cgcccgccga gatggaatcc atcccggctc gattcaaccg ctcattccat
    11341 tcaaaaacct ggtccaggtc gctgtgcatc agcagaccgc tcagcgccag cacagtctca
    11401 tattcgggct tgtgcgtctc gccgccgcct ttcaggctgc agtagccgcc gcaccccagc
    11461 gggcaggagt agcagtgata tttaaccgtc tcacgggcga tgattcggtc gggatccagg
    11521 ttattagccg ttttcccttt gaaggtcgcg cttgacccgc gccagttctg aatgggggaa
    11581 tcgcccatct cgatgctggt ctgattcatc cccgttgtgc cccacttccg caggatgacc
    11641 ttgtagagca gcccatcctg tcgcatactc agtggcatca cccgcagcag ggtacccagc
    11701 agccccatgc ccttgcccgg caaaaatggc ggttggaatg caatccagcg gttgaatcga
    11761 tcgctcaacc gctgcacctc gccagcatca tgaaccggaa tccggtaggc tccgtgcagt
    11821 gcaatggctt tcagccgttt ggcgcccatc accgcaccca gcccggaacg cgccgccatc
    11881 cgcccctggt cgttggatat gcctgcaatc aacgagcatt tctctcccgc catgccaatg
    11941 caggccacgg ccgccgggcg gccctcaaac cgttcccaga gtgccgtttc ggtttcccgt
    12001 gtatccttgc cccacaggtc gctggcatct cgcagttctg catgtccatg ttccacatgc
    12061 aaataaaccg gcgtcgggct gatcccggtg aagaaaatcc cgtcatagcc gcattgtttg
    12121 atcgccggcg ccagcgtacc gccgcagttg gcatcgcccc aggtgcccgt caatggcgat
    12181 ttagccgccg ccatccagcg cccggtcatc acgctgccgg tcccggtcag cagccccgag
    12241 acaaacccca gcacgttctc cggccccagc gggtctgccc ccggcggaat ccgctgatag
    12301 agcaggtagg cggccaaccc cagcccggac agcacttttt catatacccc atctgggatc
    12361 tgctcctctt caatttttcc ggtggtcaaa tccacccaca gcaccttgcc catgacagcg
    12421 ttcatcatat tatcctgtct atccaaaggc ttctcgagcc agcttcaggg cgtcttccaa
    12481 tcctagttcg gtgggattca tcatcgctga cccgtcatta attgccagct ttgcaatgtg
    12541 ctcaagctga tcggccgata ccccggcttc cttgagtgtg cgtggcagtc cgctgatttc
    12601 gtacaggtct tgctttagcc ggtgcacctc gtcaatggtc ttttgtgcgc gctgagcggc
    12661 tggcgtcgct gcataaatct ctgccccggc cagcggcagc agcagttcgg caatctcggc
    12721 ttctcgcttt ggcaagttaa acgccaggcc aaacgggaga aagatattca tcgcaatccc
    12781 gtgcggcaca tgcgccactg ccccggtagc atggcccatt gaatggacac accccaccat
    12841 cgaattagag aatgctgccc ccgccagcag gctggcattt gccagcgcca gccgcccgtt
    12901 tttctcctgc ggattggccg tcaccacccg caaatttgcc ccaatcagcc ggatggcctg
    12961 ctgggcaaat gcgtcactca ccgggttctt ttgaatgctc aaacaggctt ccatcgcgtg
    13021 ggtcagcgca tccatcccgg tggcggcggt caatttgggt ggcagggtca gcgtcatccg
    13081 ggtatccagg atcgccgccc gcgggatcaa gttctccgag acgaacagca tcttcacgcc
    13141 ggtttccggg tcagcaatca ccgctgaatt ggttgcctca gagccggtgc ccgaggtggt
    13201 cggtgccaca aacatggggc gcagtggccc cggcaggacc tctgcaccgc gataatgccg
    13261 cacatcgtcc ccgccctgtg ataccaggat gttgatgccc ttggcagtat caattaccga
    13321 gccgccgccc accgcaacca ggcaatcgca ggccttctcg cggtacagtc ctgccagctc
    13381 gttcaccacc agcgtagatg attcgggcgg cacctggtca tataatgccc caatggtaat
    13441 cccggactcg gcaaaagcat ccagcaccac cgtaatcaaa cccgcccggg ccaccccggg
    13501 atcggtgata atcagaggtc gctccacatt tgccagccgc agttcaaccg gcaagttgtt
    13561 caacgccgtg tgtcccgaga tgattttgac cgggaatgca aattcgtaat aagggggaag
    13621 catcatggtg aacctcctgg tttagatgcc aaatggaatc ccgagcaaat aggtcttcag
    13681 ccgcagccct tgtttccgcc agaaggggat ggctggcagc cgcttgacca ggtgctttga
    13741 gataatggac gggaatagat aacattccgt aattcccagc gcccgtacca ccgaaaccgt
    13801 attggacagg tcgccgcggg cgcacatgca gtgttgcgca taggcgacat caatcccggt
    13861 ttgggccgtg aacatcttga aagctgcctc aatggtcttg aagtaaatca ccacgtcggc
    13921 tttgttttca tcaaaatgtg cacccatata cttgaccgtg ccgtcgggtt gtttttgcag
    13981 cgccaccgag ctgccattag gctgcagctt catcatcagc gtcagccctt tgggccagcc
    14041 gctcacctca tgccgaatca gcggctcgct gtgtgacagg gattgaaagg cccgtcccac
    14101 cacaaacatc gccgccgcaa tcaccgcttt ctggagcggc ttctggcggg ggggcaggtc
    14161 ggggtagatt tttgccgaaa tcatgtcacc tccttacagt gggctgccag ataggcagca
    14221 atcgaattta agggaatatc cgcaattccc aagacagact gaatcacaaa tccatccagc
    14281 gttgccagga tgatctgcgc caccagctcg cggtcctgct caggaatttc cacctgcgcc
    14341 agaccctctt gcagcatctc gcgccactcc cgatactttt gtatgaaccg ctcacggatt
    14401 acgccattgc tggtcagcgc ctgctcaatc aggtagtgat gcagccgtcc gcgcagttca
    14461 tccttcacaa ttgtctggaa taccacgccc aaaatttccg ccggtgtcat gctctgatgc
    14521 gcctgccgca cccagccaag cagctgcgcg gtgatccggt cgaagtgccg gtctgtaatg
    14581 tcaaaaatca gttcgctctt ggttgcatag taataaaaca acgtcccggt gctgatcccg
    14641 gcgcgggcgg caatctctcg caggctggtt tgttccacac cctgttctgt gaacagcgca
    14701 ctggccgcgt ttaagatatg ttccttatta gacatttcct ttgacgaact gcgtcttgct
    14761 gccatacgct tcctgtctga gttgtgttgt gcgggggaat atgtcggtcg gttgaccgac
    14821 tgaatttagt ataccgactc tcaaccctgc tgtcaagaac ccaaaagcgg cgagcactgc
    14881 tcgccgcttt ctgaggaggg aagtcgccga agttatccct tgagcgcgcc ggccatcagc
    14941 ccgcgcagga agtaattgcc caggaagatg taaatcaaca gggtgggcag cgccgccagg
    15001 aacgagcccg ccatctgcac attccacgag ataatctggc tgccagccat gttgttcaat
    15061 gccacggtca ccggccagct ttgcgagccg gtcagcacaa cggcaaagat aaaatcgttc
    15121 cacgccgagg tgaactgcca gatcagcacc accacaaatg atggcagcga gatcggcagc
    15181 ataatcttga agaaaatccc cataatgtcc gccccgtcta tgcgtgctgc ttccagcagt
    15241 tccttgggga tggtggcata gtaattccgg aaagtcagcg ttgtgatcgg gatgccataa
    15301 atcacatggg ccagaatcag accgctcaca ccgccgtaca ggttgatttc cttcataaac
    15361 tgcaccagtg gaatcaaaat gctttgatac ggaatgaaca tcccgaacag aatcagcggg
    15421 aagatcacat ccgcaccctt aaacttccac ttcgaaagca catacccatt cagcgaaccc
    15481 agcaccgatg atatcaaggc cgccggcacc accatcaaga tgctgttttt gaagtacggc
    15541 gccagcttgg taaaagcctc aatgaaatta tcaaagtgta agccgttctg cggcaggctc
    15601 cacatggttt gcaagtcaat ctcttcaaag ctcttgaagc cagtggtcag catcacatag
    15661 agcggaacga gatagaatat gaacattgct acaagcggga tatacagcag caggcgagtg
    15721 cgtgagcgtt tcataagctc tcctccgttt tcaggctgta acgcaggtaa ggaatgacca
    15781 gtaccgccac actgattaac agcaaaatac tgatcgccgc ccctcggctg tagaagccgc
    15841 catcaaacgt ggtttgccac atatacacag caggtacatc cagctgcagt tgtttgccgg
    15901 caacggcaat aatcaaatca aacaccttca gcgaaatatg ccccagaata atcatcgcgc
    15961 tgaaggtaat cggctgcacc agcggcagaa tgatgtgccg gtagatctgc cattcgttgg
    16021 cgccatccac ccgggcggcc tcgcgcagct cctgtggaat cgagcgcaaa ccgctcaaat
    16081 acagcgccat cacatatcca gacatctgcc agatggctgg aatcgcaata aatgctatgc
    16141 cccaaccggg tgtcgtgtac caattgttga ttaaaaaatc aagccccagc ttgtcaaaca
    16201 gcaggttgaa cccgctcatc cgggttccct gggccgggtt catcaaccat cgccagacca
    16261 ccccagtcac gatataagaa atcgccatcg ggaacagaaa aacggttcgg aagatggact
    16321 cgcctttgag atcctggtcc agcaaaatgg ccaggaacaa acccaacagc aatgcgccag
    16381 cgacaaagag taccgtaaag agggctgtgt tgcgaacatc gacctggaag cgcgggtctt
    16441 taaacagttc aagatagtta ctgagcagtg gcttggccca ggtgtaattg ggcatcaacc
    16501 ccttccactg actcagcgaa accttcgccg accagccgat gaagccgtaa ataaagatgg
    16561 ccaccgccac aatagatggt aggaccagca ggaagccaat caatcggtct ttgtcaaatt
    16621 ttcggcgcat aaactgcctc ccgtaaatcc atttggggcg ggtgtgaccc cgccccaatg
    16681 gtgtcaaacg tcagatttac ttgcaggggc cagaggccat gcaggccgcg accagagccg
    16741 tctggaaggc cgcgccatct ttcgagctca ggaacaggcc cagagccgta tcgatttcgg
    16801 atttccacgc atcgttggcc accacaccgt gcgtcaggct gccggtgatg acgttggctt
    16861 tccagtcatc catggcagag agcaggtatt cgccatacag gctgcggtcc gcatcgttgc
    16921 gggcggggat ggagcccttg acggggttga acgcatcctg accttctttg gagccagcaa
    16981 cgcgcagcca ggcaatcgcg gcatcgcgat gcggggcgcc cacagccagc acaaagctgt
    17041 cagagaggaa ctggaagctg ccaaccgtac ccggaacagg cgcccaaccg taatcggtct
    17101 tgggggcctt gcccagctca cggaagtaac cttccgccca gtcgcccatc acattgaacg
    17161 cagcgtcgcc gttgatgacg agctgagagg catcctgcca gctcaaagag gtggcatcgc
    17221 tgttggtgta gctcagcagc ttgatgtagt tgtcaatcgc tttggtcact tcggcgctgt
    17281 cccaggcggt ggttccgtcc cacaggccat tgtaggcatc gggacccagc gtgcccagca
    17341 gcacggtttc aaacaggtgc atggcagtcc actgctcgcc catcgccagc ggggtttcaa
    17401 cgccagcagc cttcagggca tccagtgccg caaaccactc atccatggtg gtcggaacag
    17461 caatgttgtt ctcggccagg atggtcgggt tgtaccacat cacgttggcg cgatggatgt
    17521 tcaccggcac cgaatagatg ttgccgtcct ttgagatgag cgggatcagg gtctcgggca
    17581 tcacatccag ccagccttct tcttcataga ggaagttcaa ggcttcaatc tgtccggcag
    17641 ccacatacgt gccaatcagt tcctgtccag catggccctg ccagctgtcc ggcgggttgc
    17701 cggcctgcag acgggtcgcc agcacagcgc gggcattggt gccggcgccg ccagccacag
    17761 ccgcatttac gaattcaaca tccggatact tggcctggaa gattttaatc atggcatcaa
    17821 gaccggcagc ttcaccgccg cccgtccacc aggagaaaac ttcaacctga tccgaggtgg
    17881 gttcttccac cttgggggtg caggccgaaa gcacaaacgc ggccaccagc aaagcgctga
    17941 tcaaaacaaa cagtttctta gacatttcaa ttctcctcaa agatattcta gttgaatatt
    18001 gacgttctga ttgctgcaaa tggggtagca attgttttac gaaccacctc cttatgagta
    18061 cacctgggct gcctgtctgc cgttaagcgt tttggcaagc tgcgtgggtt caaatgcatt
    18121 ttgaataacc agcgtcgcag ccccgattgc aatggcatca ttgccaagtt ttccggcgac
    18181 aatcgccgct tgttgggcgg caatcggcat aacgcgttcg cggatggagg catgaatcgg
    18241 ccccatcaac aattcgccag cctgcaccag ttcgccgcca agtatcacca gccccgggtt
    18301 gaccaggttc agcaggttgg caattgaagt gcccaggtat cgcccggcat tttgaatcac
    18361 ctgcctgcaa accgcatcac cctgcagggc ggtatcgacc agatcttcaa ccgtcccggg
    18421 catttcatct gccccgcggt gggcagcctg caaagccgcc tgcgcatccg ccagcagtcc
    18481 gggcccgccc acgtaagcct ccatgcagcc atggttgccg cagcggcaaa gccgcccatc
    18541 gacttcgatg gttgtatgcc caatctcacc ggcagtcccg ctctcaccac gataaatctg
    18601 gtggtttatc accaggccgc tgcctacgcc aatcccaatt ttgatgtaag ccaggtcggn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttatcaccag gccgctgcct
    18781 acgccaatcc caattttgat gtaagccagg tcggcaaatt cttgcccgct gccccaccat
    18841 ttttcaccaa tcgcgccagc gttggcatca ttttccagat agaccggcag ccccggaaag
    18901 aaagcccgga tctttggctt gggtaaaatt ccttcccaga ccggcatgta atacgtcacc
    18961 aagcgatcca gcgcttcacc gcccagcggc gtgggcactg taaaaccgac ccccagcaaa
    19021 tggtcaggct caatctgggc ctgtttcagc gcctgggccg tcaatgtttt gatcacctca
    19081 aaggtcaatt ccggctcgtt gatcacatcg cagcgctgtg tctcgcgcga aatcaccttg
    19141 ccgctcaaat ccatcacaac cgtcgtcaca tgcgacgccc ctatgtcaat tcccagtgca
    19201 tagcggtact ggtaattgaa ttccatcagt atcggccgcc gcccgccgct ggattcacca
    19261 atcccggctt cgcgcacaaa gccgctctca atcaaaacat ttgccagcgc cgaaaccgtt
    19321 gtggcgctta acccggtgcg gcggatgatc tcggctcgcg agatcgaccc ggacgcatgg
    19381 attaaccgca gcaccaggct gaggttattc tctcgaatga tggcagcaga agcagggcgg
    19441 gtgggcatag ggtttttgag gaaaataggg ggtaaaaaac ggacatttat gattatagcg
    19501 acggcaactt actttgtcaa gtagccaaag aaagtttaag aaattcggtc acaatccaaa
    19561 gtatgacccg gctatagcaa tccagccctt caggtgtgac ccatggcaca tcccaatttt
    19621 tgctccgcag cctatactga tgctgacaaa aattctcccg gaggtatcca ttccatgcgc
    19681 gtcctgatgc tttatcctga atttcccgat acgttctgga gctttaagca cgccttgcgg
    19741 tttgtgcgca aagcggcctc ttctccgccg ctgggtttaa tgaccatcgc ggcgctcctg
    19801 ccctccacct gggaaaaacg ccttgtcgat ttgaatgttg cccgcctgcg tgaccgtgat
    19861 attgcctggg ctgatgtcgt catggtctca gccatgatcg tccagcgcca atccaccatt
    19921 gaagttattg agcgctgcaa gcaggccggc aaaaaagtca ttgccggtgg tccgcttttc
    19981 ctcgctgaat gggagagctt ccccacggtc gatcacttca tcctcaacga gggtgaaatt
    20041 accctgccgc tcttcctcag cgacctggcg cgcggctgcc cccagcgtgt gtatcagacc
    20101 gatgagtttg ccgacatggc tcaaacgccc accccgctct gggagctgat tgatctttca
    20161 aaatacgatt cactcagcat tcaatactcc cgcggctgcc ccttccagtg tgacttttgc
    20221 aatattaccg ccatgctggg ccgccgtccg cgcaccaaat cggccgctca aattattgcc
    20281 gaactggata aaatgtatga actggggtgg cgccgcaaca tcttctttgt ggacgataac
    20341 ttcatctcaa accgccgcca gctcaaagaa gagattttgc ctgccctcat tgcctggcgg
    20401 aaaggcaaag caagctgcaa ctttttgacc gaggcttcca tcaatctggc tgatgacgag
    20461 gaattgatgg atttgatgac cgcggctggg ttcgtctcgg tctttgttgg catcgaaacc
    20521 cccgacgaag gcagcctgac cgaatgtaac aaaggccaga acatccaccg cgatttgatg
    20581 gacagcgtcc attgtttgca gcgccatggc ttgcaggtca tgggcggctt cattgttggc
    20641 ttcgataacg ataccgaaac cacctttgac cgccagatag atttcatcca aaaaagcggg
    20701 gttgtcaccg ccatggttgg tatgctgcag gccattcccg gcacaatcct ccatgaccgt
    20761 ctgaccgccg aaggccgcat tgcccaccag gccaccggcg ataatgctga cggcaccacc
    20821 aacatcatcc cccgcatggg catcgaaagg ctccaggctg gctaccgccg cctgatggcc
    20881 gaaatttaca gcccgcgccg tttttatgag cgcgtcaaaa cctgcctgga gctttacaaa
    20941 ccctccaacg cgcccgtcca cctgcaattt gacgaaatct acgccttctt gcgctcaatc
    21001 tggaaattgg gtatcattgg cgaagagcgc cgcgaatatt ggaagttatt cttctggacg
    21061 ttatttcgcc acccgcgcaa gctggccctg gcaatcacgt tcaccatcta tggcttccac
    21121 ttccgccagg tcaatgctct aaacggtatc tcatgagagt tgacaatgcc cccttattcg
    21181 attggtttaa ttacagatag cacgtgtgat atccccgccg aacttcggca gcaatatcaa
    21241 atcggtatcg tccccctgac ggtagtctgg ggcggcaagc aaatgctgga tggcgtggaa
    21301 ttgtccgcag atgcttttta tgagcgcctg gtcaaggatc ccgaaatccc caccacctct
    21361 cagccatccc ccaaacaatt tctcgaggcc tttaaagccc agcaggaagc cggcgcccgt
    21421 gaaattgtgg tcatcaccat cagcagcgcc atgagcggca ccattgaatc cgcccgccgc
    21481 gccgccgaga tgatggatat ccccatccat atctacgact cccgctcaaa ttccatgagc
    21541 ctgggctggc aggttttggc tgctgcccgc gcccgtgagg ctggcggaga tgccgccgcc
    21601 atgatcaccg ctgccaatca ggcccgccag cgcatggtct acatcatcgt cctggatacc
    21661 atggattacc tggtcaaagg tggccgcatt ggcggtgccg tccgattcct cggttctttg
    21721 ctcaaaatca aaccccagat cagcgtcaat cacgaaaccg gcaaagtcga agccggcatc
    21781 ccggcccgct cccgtgacag cgccatcaac ggtctggtgc gagacttttt ccgccgcctc
    21841 aatcctcagg gcaaactcca cctggctgtg ctgcacaacg ctgcacccgc agatgccaca
    21901 gccctcgccg agcaggttcg ccgcgaccac gcccccgccg aactggtcat gtccattgta
    21961 tccccctgtc tgggtgtcca caccggcccg cgtgccattg ccctgtgcgg ctatactgaa
    22021 gattaagtcc aggcaatttg ccaaaaacct tgacattttg cccccaaata ctggattaat
    22081 ttgcatgatg ggtgtataat ccgcccttgc attcaccact cgttggcaaa cgcccaaagt
    22141 tcggcctcga tgttgcgtga aagactattt atcagacgga aacgtctacc agaagaaaag
    22201 gatgatggag aaatgaatat ctacgttggc aatctgccat tcagtgcgac cgaggatgaa
    22261 atccgggcgt tgtttgctga ttacgggcaa gttacgtcgg ccgcagtgat caaagataag
    22321 ttcacgggcc aatcccgcgg cttcgcgttc gtcgaaatga cgagcgacac cgaagcccag
    22381 aaggccattg ctgaactgaa tggcaaagac ttcaagggtc gcgccctggt gatcaacccc
    22441 gctcgccccc gcgaagaacg ctccgatcgt ggccctcgcc ccggtggttt cggtggtggt
    22501 ggcgatcgcc gcggtggtgg tcctggccgc tccggcggtg accgccgtgg tggtggccgc
    22561 actggctggt aaacttaaac tttagcaaga cttcaaatca gccctcagga taccctgagg
    22621 gctgattttt tgtattccct ttgtctgctt tcaaaaatta ttacaaatcc gtccaaaatt
    22681 gcccccgaaa cctgttgacg agattagaaa atatcactat aatattaatg taaaattaat
    22741 attactgctg tgccctacac catctttgtc gctgactgac attgctgggg catcggtgga
    22801 tgctttgatc tacaggcgaa cccctggtcg cagttctgcg caagcagatt aacctgtaag
    22861 gagcgaattg cgaaaccggc cacgcagccg gttttgttat tttaatggcc cggctgctcc
    22921 accgccttcg ttccctactg gtaattaatc tgtctctctc tttgtgcgcg tattggggag
    22981 gccctggatg atatttaaag attatctcct gattgtctgg cggagacggt ggattttgat
    23041 attcaccatc ctcgtgacgc tgtccgttgt tgctgtcggc acctatctga caccgcccac
    23101 gtatgccgcg tcggccattc tccgggtggc cactgcttcc agcgggtcgg tctcttacac
    23161 agattatgtc tatgccgacc gtctggtgaa tacctatgtc catatctcta ccagccgccc
    23221 ggtattggac gaactcgctg tccgtctggg gcgcacagaa gcccccgaag tccaggttca
    23281 gattgtcacc aataccgaac tgctcagcat ctctgttgaa gacagcgatc cgcagcttgc
    23341 tgccacggct gccaataccc tggcatccat cctcatcgaa caaagccaat cgctttacac
    23401 tggcggcggg cgtgacccca aagacatcct cgccgaccag ttgacccaga ttgaggccga
    23461 actcactgct gcccgcgccg cctacgatga actggtccgc aattcccctt ctgattccga
    23521 ggccatcgcc gcggccagca gcgccattga cctgaagcag aacacctacg ccacccttct
    23581 ggaacaatac gaggaagccc gcctgagcga cgccctgcgc gccaacacca tctcaatcct
    23641 tgagtcagcc atcccacccg aaaagcccat ccgtcccaac gttctgatta actttggcct
    23701 gggctttatt cttgcgctcc tggctggcct ggggctggtg tttgttttct cgcactttga
    23761 taccaccctc tacaccacca gccagattga acgcctagtt gggcagtctg ccctggcgca
    23821 catccccgtc acccatcccc agcaggtcct cttaaccggc agtgacggcc gctcgcctta
    23881 cggccaatcc tatcgccgtt tgcgctccac ctttctcaac caacttcaaa ccctggcctg
    23941 ccagacggtg atgatcactt cacccgagcc tgaggaggga aaatcaacca ttgcagtcaa
    24001 cctcgcagtc accttggccc aggcggggcg ccgggtcatt ctggtcgatt ttgacctggc
    24061 gcgttctcag gtcgcccaga tcctcaatct gcccaatgaa cttggtctgg caaatgtatt
    24121 gaccggacaa accgaattga actcggcttt gcaatccgcc gcctgtccag gtttgcgggt
    24181 actgaccagt gggacctcag cctcggaatc gggtctgtat ctcaacagca gtctggtcgg
    24241 cgacctggtt aatcaactgg ctgcccagtc agatatcgtg ctgtttgact cccccgccat
    24301 tctgctcatc tcggattcaa ctgacctggc ggctgaagtg gacggtgtgc tgatggtcgt
    24361 tcggcgcggt gccacccgcc aacccgctct cgaagaagcc cgcagtctgc tggataaact
    24421 aaacaccccg attattggct atatcgtcaa ccgcgccgag gccaatcacg ctactgctta
    24481 ctacacccgc ccccggcaga ccatggggcg ccgccgctta taacccccca ctattcccgg
    24541 gacgtgaggc acgtcaggag gacggtgaca tgcagacgga taagctttcg gaaattgaat
    24601 cccaacctgt tcccaggagc agcagcaggt cgcggcaggc ctactacctg atgaaacgct
    24661 gtgtagatgt gctgctgtct ttcattgtcc tggtcttgtt gttaccaatt ttgctggtgc
    24721 tggccctggt cattcgcctg gattcccccg gcccggtgat ctatcaccag acgcgggtgg
    24781 gtgcccggcg agtgggcggg cagtggcagc gggctgattt taccttcttc aagctgcgta
    24841 ccatgcgtca gaatactgat gcctcgctcc accaggccta tgttcatgcc ctgattcaaa
    24901 acaacactgc tgaaatggcc gccctgcagg gcgcagagat gggcaatgtc aataaactgg
    24961 tgcatgatcc acgcatcacc cggatcggac atttcctgcg caaatacagc attgatgaac
    25021 tcccccaatt ctttaacgtc ttgcgcggcg acatgagcct ggtgggcccg cgccctgcac
    25081 tgccttacga gatttccgac taccagccct ggatgctgga acgcttgcaa gcagcccccg
    25141 gtatcacggg cttgcagcaa atcaccgctc gctgcaccgc cgactttgca tcccaggttc
    25201 gcctggatat tgaatacatc caccgccaga cactctggct cgattttgtc attcttttca
    25261 aaacgccttt cgccgtcttg cgggcggagg gcgcgtgcta atgcatcaag gaggctaatc
    25321 atggataaac ttcgtatcgg tgtcattggt tacggatact ggggaccgaa tctggtgcgc
    25381 aactttgtcg aaatacccgg tgcagagatg attgccgtct ctgacctgcg catagagcgg
    25441 ctggcccagg caaaaagcat ctacccatcc cttcaaacca ccaccaacta ttttgatctg
    25501 tttgacatgg acctggctgc cgttgtggtg gcaaccccgc cgaccacgca tcataacatc
    25561 gcccgcgact gccttgagca tggtctgaac gtcctggtgg aaaagcccct caccctcacc
    25621 agcgatgatg ccctggcttt atgcaaccag gccaaagcca gcagcctgac tctgatggtc
    25681 gggcacacct ttgaatataa cgccgccgtc catgcgcttc gtaaactgat gagcgatggt
    25741 gacattggcg aagtccatta catcgacact gcccgcctca accttggatt gttccagcgg
    25801 ggcctcaatg tgctctggga cctggctccc cacgatatct ccatcctcct ccacctgctg
    25861 cagcagcccc cagtttcggt cagtgcccat ggcatggcct gtgttttcaa agatattcac
    25921 gatgtcgcct acatcaccct gctcttcccc aatgatgtcc tggctcatgt gcatgtcagt
    25981 tggctggacc cctgtaaggt gcgccgcgtg accgtcgtcg gcagcaagaa gatggtcgtt
    26041 tataacgata ttgaagccca ggagaagctc cgcatttacg acaagggcgt cgatggtccc
    26101 ggccttagtg aatctcagga cgatttccgc tgctcctatc gttacggcga catcatcatc
    26161 cccaacatca aattgggcga gcccttgcgc gctgaatgtc agcactttgt agattgcatc
    26221 cagaaaaaag aaaaaccctg tacctgcggc atggaaggct atcaggtcgt gcggattttg
    26281 gaagccgccc agcgctcgat gtccaactcc ggacatcagg agatcctgga atggtaaccc
    26341 cagaatttgc ccgcatcgcg cccgatgttc gcctgggtgc caatgtccgc atctacgcct
    26401 tcgtcaatct ttatggctgc cagattggcg atgacagccg catcggcacc tttgtcgaaa
    26461 ttcaaaaggg tgtctcggtg gggcagcgcg tcaaaatctc cagtcatacc ttcatttgcg
    26521 aaggtgtgac cattgaagac gatgtcttca tcgggcatgg cgtgatgttt atcaacgaca
    26581 agtatcccca tgcttccacc gcggccgggc agcctcaaac cgaagccgat tggatctgca
    26641 cgcccacgcg ggtggagcgt ggcgcatcca tcggcagcaa tgccaccatc ctttgcggcg
    26701 tgaccattgg cgccggcgcc atggtgggcg ctggcagtgt cgtcacacat gctgtcccgc
    26761 cgggagcggt tgtggccggc gtgcccgcgc ggatcttacg aaaaccatcc caatcttgat
    26821 tttttcactg cggaggaaac cttcatgtcc cctatccaat ttgtcgatct aaaaaaacag
    26881 tatgcgcccc tcaaagagga aatcctggct ggtgtagctc ggattctgga tgggatgcag
    26941 ctcttcctgg gcgaaaacgt ccgcgccctc gaaaccgaat ttgccgcctt ttgcggtgcg
    27001 tcttacggca tcggagtcag cgatggcacg accgccctgc acattctcct gcgcgccatg
    27061 gatattggtg ctggtgatga ggtcatcacg gtctcccatt ccttcattgc cacggccgaa
    27121 gccgcctgcc tggcctgtgc ccgcccggtc tttgtggata ttgaccccca gaccggcctg
    27181 atggacgtct cgcagattga agccaaaatc accccgcgca cccgcgccat cttacccgtt
    27241 cacctctacg ggcaaatggt ggatatggag cccctgctgg agattgcccg ccgccataat
    27301 ctccgcgtga ttgaggatgc ctgccaggcc cacggctcag aataccgcgg ccgccgggcc
    27361 ggcagtcttg gggatggcac tgccttctcc ttctactact ccaaaaatct gggcgcttat
    27421 ggcgaaggcg gcttcattac caccaacgac ccggaaattg cccgccgctg ccgcatgatc
    27481 cgcgatcatg gctccgaggt gcgctaccag cacgccctga ttggcctcaa cgggcggctg
    27541 gatgaaatcc aggcgctggt ctgccgcgtc aagcttccat atctggcaga ttggaacacc
    27601 ctccgccgcc agcatgccgc ccgctatgcc gaactgctcg ccgatacgcc cgtgaagatg
    27661 atggtcgagc gccccgagtg tcggtctgtt taccacctgt ttgtggtgcg cgcaccgcgc
    27721 cgcgatgaac tgcaggccta cctcaaagcc cagggcatct tcaccgggat tcattacccc
    27781 gtcccgatcc atttgcagcc tgccatggcc ttcctgggct atcgccccgg cgatctgccc
    27841 gccaccgagc aattggtggg cgaaatcctt tctttgccca tgttcgctga gctgactgag
    27901 gacgatttgt ccaccgtctc cgcagcaatc catgcgttct atcagggata atcgttgcgg
    27961 tctggaggcc gcctgaaatg cgccgtgctg aactgctggc cgcgctggtt gttgggctgc
    28021 tccttggagc cgtgattgtt gctgccgcat tgctgcagaa caaatcgccg cgcccggtca
    28081 ccccgcgctc tgccacgccg ctgcagtcgg cctctgctgt catccgcacc ccaacgccaa
    28141 cccccatccc ttccgctgtt gctccggcag ctacactaac cccttcgggc cctccgctcc
    28201 tcgaagccga tccccccggc accgatgccc ccgatgttct actcctgttc aacccggctc
    28261 gcgcctccag cttcggcacc aacttctgcc gcctggcaga attctatgga ttggtgtgca
    28321 cccaattcaa cgtgacgcaa agggacaccc ttaccgccgc cgatttgctg ggccctcagg
    28381 gtttacccta ccccctgatt gccctggatg ccaccttgct ggataaccgc tccccattgc
    28441 tctcatccgg cgaccttgaa gttcttatgg aggcagtcaa tcagcagggt gtctcggttt
    28501 tggtgggcaa actggatgat ttacaaaatg cagatctggt ttctgccctc accgccgggg
    28561 ctgtggcagg cgtaacccgc cccgaagata ccaccaaaga ctggcaggtc agccgccttg
    28621 cacccgaaat cacgcttgag ctgaccgggc agaacatcat cacatctcag gctgccactc
    28681 ctgacacctt tgccctcaac attgtcgatc cgctccccgt gaccgttctg atttcttcca
    28741 ccgataacag cggcgcttca tatcccatct ttctccgctg gcagccctct gatcaacccg
    28801 ccgcgggcgc tgtttacctt gattccggtg aaaccggtca gtcgctggat tttatcgccc
    28861 tgcgcgaagt ctattactcc gccgccagct tctctcagat catccccacc atgctgacct
    28921 tgcgtcatgt gattggggag gagacctggc acgctcccca ggattttgcc aatctgacca
    28981 ttgacgaagg cgccctgctc gaaccctacc aggatttgaa ctacaccgcc ctgctcgaat
    29041 taatgcgccg caacagctat cacaccaccc tggccctcat cccgcaaaac tggcagttgt
    29101 ccgagtctga tgttgtggcc ctgtttttga ccgccccaga ttatttctca ctggcccagt
    29161 acggcaacac cggcgctggt tatgaatttt accgttacgc cacacctgcc cccgatgcca
    29221 ccgatgtgcc gctgctgccc gcccgcccac tcgatgacca gcagcgcagc attcttgaag
    29281 gcctggcgcg catgcagctt cacgaaaacc tgacccgcgt cccctttgat cggatcatga
    29341 tcttccccgg cggcatcagc ccggttcaga cgcttgaatt tctaaaggcc aacaactatc
    29401 tggcaaccgt caacactcag gacctgccgc ttgatgcggt ccgtcccacc aactgggatt
    29461 atggcatgac ccccgccatc accgactttg ccggtttccc caatctcatc cggcgcatgc
    29521 caacagaaaa tcaggcctac cagccttata ccctggcctc catcctggac ctgctggtgg
    29581 ataaacccgc cctgttctat acctatccct atggcagcgg tctgttttct gccggaatgg
    29641 acgcgtttga ttcaatcgcc gggaagatga atgccctgga gggcgaggta tcctggcgca
    29701 gcctgggtga gattgccaaa cggctatgcc ggattaaacg cgatgatgac ggcaccgtca
    29761 gtgttcagat gtacacgcgc tggctgatcc tggcgaaccc ggacgctgca gacaccgtct
    29821 ttcacatcac caaatccgag ccgctcaacg ttcccattgt ccgcctgacc gtaaatggct
    29881 atgccttccc ctatactatc caggatggca gcctgacgct caatctgatc atcccggccc
    29941 aatcttctgc ggagattatc attgaatatt cccaaccggc tgaataactg gatctggcgc
    30001 acaactcgac tgtcgcccaa agtctggctg gcgcaaatcg gcttgctggt gcttgccagc
    30061 atcctgattg gcgctctggc tgtgctgcaa gaccgcctgc ctcaaaaagt cggttatctg
    30121 gtggtcgttg ctgccgcggg cgcctgcact ttgatgatga ttggcaacct caaaaaggtc
    30181 ttgctggccg ccatcctgat cgatattccc ctgcgttggg atgtgtacct cggcgcccgg
    30241 gtggtcaact cgcccgtggg catgcgcgaa ggctggatga ttggcctgac aactctcgcc
    30301 ctggctggcc tgtttggcgt ctatctgctg caagaattgg cccgcccgca cagccatccc
    30361 cgcatgcgct ttgttgataa cctgcctctg ttcctctaca tggttgtggt ttgcctgtcc
    30421 acctttgtgg ctgcagatac ccgcggtgcc ctctttcaga tctttttcct cgcccagatg
    30481 tatcttctat atgtctatat cgtcagcgtc accaaaaccg cccaggatgt ctggtttatc
    30541 atgctgtttc tgctggccgg actggccctc gaaggcctga ttgtcctggc ccagcgcttc
    30601 ctgggtttac agattaacct gctgggcatt gtgaccgtga atagttatgg ccgcatggcc
    30661 ggaacaatgg gctcacctaa cgttgccgct ggtttcttca gtctgttgct ggcccctgca
    30721 ctggggctgg ccttcacgcg catcccaacc ctggcgcgcg gcggcgccct gatcgccttt
    30781 ggcctgggtg gcctggggct gttgttcacg gtttctcgcg gcggctggat ggccttcgcc
    30841 atctctttga tgtttttggg cctcatgctc tggctgcgcg gcaaaatttc tggcggcgtg
    30901 gtgattttgg tgatgtttgt cgccgtgatt gggttggtgc tgtttggcgg cgtcttgttc
    30961 gataagctgg ttggcatgcg caccaatgct gccatggcgc gcatcccgct catgaagctg
    31021 gcctgggaga tgatcagcga ccgacccttc ctgggggtgg gcgccaataa ctatgtgatg
    31081 aacatgaagc agtacatgat tcccgaactg acccgcggct tcctctatgt cgttcacaac
    31141 aaatacctgc tggtctgggc tgaaactggc ctgggtggca tcctgaccta tctgggcttt
    31201 ttaggcctca cactctggcg cggctggcag tgctggaacc gtgaccatcc cctcttctcc
    31261 cccctggccc tgggactcat cgccgccatt gtcggtcaga tggcccacat gatggtcgag
    31321 atatttgatg cccgttccca ggtccaatcg ctctggctgg tggccgcgct ggtctcagct
    31381 ctggaattga tgactcgtgg aggcagccat gaatccgcct gatgccgccc catctgtcgt
    31441 tcattcccgc acccgcgccg ctcgtctgat tggcaacacg gcctctctgg tgacgaatga
    31501 cctggtcaac cgggcaatca cctttgttct ctatgccctg attgcccgtt accttggcgc
    31561 ttacgaattt gggcaaatgt ccatcacact catcatcttc tannnnnnnn nnnnnnnnnn
    31621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31681 nnnnnnnnnn nnnnnnnnnn nngttacctt ggcgcttacg aatttgggca aatgtccatc
    31741 acactcatca tcttctacct tcttcaaagt ctggcgccca tggggctgaa gatgcttctg
    31801 gtgcgcgaaa ccgcccgcaa cccgcaaaac agcggcgcct atttggttca gggcagcctg
    31861 gtatcgctgg cggcttcaat cctagccatc tggctggtgt ttggctttct ctccattatg
    31921 aactacgccg aagacaccac ctggatcatc atgctgattt cgttcggcct catcccatat
    31981 tcactttccg cggtgtgtga agccatcttt caggcgcatg aacaaattcg ctggatcacc
    32041 cttgccaatg tcccggtcag cctgctgcgc ggtctggcag ccttctggat gttatctgcc
    32101 ggtttagatt tgcgctggat cggcattctc ttcctggtca ccttcaccat cacctggctc
    32161 ctggagtgga ttttgctcac ccggcacatt gcgcccccca actggtctgg cccgccagca
    32221 ctgtttgtgc aaattgcccg aatggccgtg ccgtttatcg gtctgcaggg catcatcgcc
    32281 atcaccaaca gcatcctgcc cctgttcctc tccaaatctt caggcgaggt gcaggttggt
    32341 ctgttcaatg ccgccaacca gattatggcg ccggttttac tggtcagtca gagcgttgtg
    32401 gtcagcctct tcccgcgcct ctgccagaaa tatgatactg gccagcaggc cctccgccag
    32461 accactgacc gcctgacgga actgctgttc gcggtcacag tgcccgccgt catcgggttg
    32521 attttctatg cccgttttgt gctgctgctg atctatgata aagagatttt cgccatttcc
    32581 gccctgttgt tacagatcat ggcctgttcg ctgttgttcc gggccatcac cagtgtcctg
    32641 gggcgggtcc tgatggccag cggacgtgag cgcaccctgc tgcgcatcct gattatcgag
    32701 accatcatct gcctggccct ggcctttaca ctcatccctc gctggggtgt gctgggtgca
    32761 gcccttgcgg gtctgattgt cacgttcttc gatcttatcc ttcacctgcc gccaattttc
    32821 aggctgcttg gcgggctttc actcgggcgt tccgcctgga aggccatcaa tgccagcctc
    32881 gtactgatca tctggcttgt ctttgctatg ggcgtccaat tgaacccctg gctgggcatt
    32941 ctggtttcgc tgtttcttta cccggtcgtc tggctgatcc tggcgctctt agagtcaggc
    33001 ggctggcggc gcctgcttga acgctacgct ctgaggtgat gatgcgtgtc ctgtacctga
    33061 ccctccctgc tttgatggat gcctcgctgt cgtttgcccg ggcaatcgcc gcgcacgtcg
    33121 agctgcatat gctgctccag gtcacgccgg gcggctggcg caccagcctg tttgatgtca
    33181 acccggtcag cccgtcctcc gacttgctcc ccgtagagcc gcttctttcg ggttacccgg
    33241 ccgcgctttc aagttactgg cgcgcctccg ccagctttca ggcggctgtc tttggcggaa
    33301 gcagcgccct gcatcctgcc agttgggctg cggctcgctc ggtgctgcgc tttgcccgcc
    33361 aacttcaacc cgacatcatc catctggaga ccaccctggg ccgcatggtc ggtgccctgc
    33421 cgctcttgcg ccgccttgca ccgctcgtga ttaccgttca cgatccctca cctcattccg
    33481 gcgaatcgcc gctgaagaaa gttctcatcc gcgcccttac ttatgcccgc gcagatcatt
    33541 tcattctgca caaccgcacg cagtcagccc aattctgcca gacctaccat gtccctgccg
    33601 ggcgggtatc tgtcaccccg ctgggtgtct ataacctcta ccgcctgttt agccagacca
    33661 ccccgctctc cgacccccac accattctct tctttggtcg gttatcccct tacaaaggtc
    33721 tggagaccct gctgcaggcg gccccgcagg cctgcttgca gattccccgc ctgcgtttcg
    33781 taatcgccgg gcgccccaac cccgattacc gcctgccctc actgccagcc ttgcccaatg
    33841 gcggccattt tgaatttatc cccgactata tcccgaatgc cagacttgcc agtctctttc
    33901 agcagtccgg cctggttgtc tgcccctata ccgatgcaac ccagtccggt gtcgtgctta
    33961 ccgcttatgc cttcggccgt ccggtcctgg cttcccgggt tggcggcttg ccagaatata
    34021 ttgatgaggg tcgtaccggc tggctggttc cacccggtga tgtcagtgct ctggctgctg
    34081 ctctggtgca agcggtcacc gcccttcgct ccgatggcga tgcctatcgc caggcaattg
    34141 ccgcccgctc cgccgccgac ctgaactggg agaccattgc tgctcaaacc ctggatattt
    34201 accgccagac ccgtcaggag tggaaatcat gaaacctgtc atcagcgtct tctttgacgg
    34261 attgaagccc gaaagcctct ctgtcatgcc ctttctcaat gccctgcccc atcagcgccg
    34321 cctggaaaca gtcctgggct attctgtcac ctgccatgca tccatgtaca ctggcgttta
    34381 cccagaccgt catgggctct ggttcctctg gcagcactcc cctcagactt cgcccttccg
    34441 ttggataacc cgcatcccgc ttttgacgca tttggattgc ctcccggttt ggatgctggt
    34501 tcaccgcctg accgcccgcc gcacccatct gacggcctac tggggcattc cccgcctcta
    34561 taatctcccg ctgcgctact ggcccaacat tgatgttgct gaaaagcgct attgggatga
    34621 agatggttac ctggagggct gccccaccat ctttgagcgc ttgcgccagg ccaatctctc
    34681 ttatgaggtc gtcggcatgg tcccgcatgt gattgatacc tcccgcctgg ttgccgaaca
    34741 ccgctttacc gaagtgcgcc cctggcacta cctcttcatc ggtgatgtgg atgccctgac
    34801 gcatcacgcc acccagaccg ccccggaggt gctggataaa ctgcgccaat ttgaccagat
    34861 tgttgctgac cgctgcgctg aaatggagcg gctggcgggc ggctacgaac tggtcgtctg
    34921 gtccgatcat gggcatgtgc cactccagca gaagattgac ctatatgccc ttttccgcca
    34981 atcgggtgac cggctctcac gctacatgca tatcattgat accaatttcg cccgtttctg
    35041 gttccgagat gaccgtgaac gccagcaggt gaccgccgtc ttggaaaaaa tccccaatgg
    35101 ctttgttcta tccccggctg atatgcagcg tgagcatgtg accatgcccg ataaccgcta
    35161 tggcgacctg atcttttacc tggatgccgg cagcgccttt tctcgcacta tttttggctt
    35221 tggcctgcgc cagaaatcta tccatggcta cctgccttcc caccccgatt gtgacggcgt
    35281 ctttctcagc acggcgccca tcgctcccgc ggtggagcgg gttcgcctgg ttgatattca
    35341 accctctttg ctggccctgc tgggtcagcc catcccatcc ggcctggatg gtcgcgtgat
    35401 ctggagttaa tcatgggccg ctacacctta cagactgatc cgcgtgttga ccagatggtc
    35461 tcagagcatt tggagattat ccgggctgcc gtcctgcgtg ccatcaatcc ccaggcgctg
    35521 ctgctggcgg gcagctttgg ccggggtgaa ggcagcgtca gcgtctccga ggctggcctc
    35581 acccttttat cagactacga ggtttgcctc atcagcccat ccccggcggc acgtctggca
    35641 gtggatcgca ttctgcaaga cctcgacggc aaaatttctg cttcggtctc gcttttctgg
    35701 aataccccgg cccggattcg taataaccgg gcgcgcaatc tgtcatttgg tcgccccaga
    35761 cctaccatcg gaatgtacga gttgaaagcc ggttcccaac tcctgtatgg cgactttgac
    35821 ctgacctaca accagattga tcctgccgcc attccaccca gcgagggtgt gcggttgatt
    35881 gttaatcgca tgatggaagt gattgacgtc tggcggggcg ggggagggtt gggccttccc
    35941 ctggcaaaac tgactctggc ctgcggtgat gcgctcttac tgatatctgg gttttatcat
    36001 tattcgtata gtcgtagggc gttgcgtttt gacgcggcct tcccgcattt tgcagatcgc
    36061 atggatcccc actttctcac cctttatcgt cgcgccgctg aacgcaagct caaccccggc
    36121 cccaatgcgc cctttgatat tactcccgag gaggtgacct cagtttcccg ggcttgccag
    36181 cagacgcttg ccattttact ggacaagctg gacgccgccc catgtcagtt agccgtgcgg
    36241 tgtgctgcgg ctgtcccgat tctgtactgc acgggcgttg ccccggccct cgacccgctc
    36301 tatgagcgcc tcatcttagc cttgcgcgcc cgccgtgccg ggcagtcaat ctactggcgt
    36361 ggtttgcccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttagccttgc
    36481 gcgcccgccg tgccgggcag tcaatctact ggcgtggttt gccccatcgc cgtccaatgc
    36541 tttccccctt tcaggccctt tacgcagcca tcccggctct tttttggggc ctgccgctca
    36601 ccaaccaacc caatccagat ctcttgaatc ttgccgccaa ctggtctcgc tggattctcc
    36661 cgtctgcggc agccgaaaat ccccttgaat taacctctgc cttattgcgc gcctggcata
    36721 ccctgggata aatccaccat gaaccgcccg gtcattctgc acctcctcaa cagcctgtcc
    36781 gattcgtcca ttacccgcat tgtggaacgg atcattcggc agcagggggc ggctgccgtg
    36841 gactggcggg ttgctgctct ggggggcagc agtgggctgg tccagcttct gacgagtctc
    36901 ggcgctcaga cgctggattt ttcggctcgt ccctcctggg agattgtaca ggcttatttg
    36961 cgtcagaatc gtgtggatat tattcacacc catacccccc atacgattat cacagcctgg
    37021 cgagccattt ccgggttgcc aaagccccac cggcctgtcc acctggccac caaacacctg
    37081 ctcacccgtc ctgctgaccg ccgctgggga gcattttatg cactggcaga ttggctggag
    37141 ctgtatttgc ccgaccatct ggtcgcggtc agtcaaacca tggcccgtca gatcacccgc
    37201 ctgcccggaa tatcatcgca ccgggtgacg gccatcccca acgggattcc tgtggatctg
    37261 tacgccgcag ccgacaaccg ctcggccgcc cgtctggaac tgggtattcc ggcagatgca
    37321 ttggtctttg gctatgccgg tcggttggat caggtcaagc ggttggatat tttgctttca
    37381 gcctttgtcg atgttctcgc tcaagtgccc cacgcccacc tgttgatttt agggcagggc
    37441 tcaatgcagt ccacctggca ggccgaggcc caaaccctcg gccttgaacg ggccatttgt
    37501 tgggctggct ttcgccatga tatcccccgc atgttatccg ccatggatat ttatgttcaa
    37561 tgctcggtca acgagggcct ctccctcagc atcctggaag ccatgtccgc ccgcttgccg
    37621 gtcattgcca cgcgggtagg cgcagccgac gaggttctcg ctcacggcga tactggctgg
    37681 ttgattcccc ccgcttcctc agccgccctg gcccaggcca tgctccgtct tgcgtctgac
    37741 gcagacctgt gccagcgtct ggctgctgcc gcctaccgct gggttggggc gcgctactct
    37801 gtcaagtcca tgacggcggc ctatttccag gtctataccc gtttgctgga ggggagggct
    37861 gatcatgcct gac
//
LOCUS       DAOPVS010000009        36974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1083_length_36974_cov_58.146131, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000009 DAOPVS010000000
VERSION     DAOPVS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 36974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36974
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1083_length_36974_cov_58.146131"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PKL11_01945"
     CDS             <1..693
                     /locus_tag="PKL11_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12389.1"
                     /translation="HEIYLSGLQFACAADGYLWAFDGALLGLWDPRPGERLTQFDYQP
                     VIPPFWAYQSLWFAVVSDAGPELVSIDPFTGDELSRLPFADLFGPDVVISRGLEAAGW
                     IWLIDTDGHFWLMSPDQVFLDFAGPSGARALAFDDYRIWVAGTEQTWRIDAGSAEVID
                     KLDLQLTALTYGNGRIWALDETGALLSLDPETAEPESFGTVCAYPSDLAWNDGLLWVT
                     CPPDQSLIALQP"
     gene            705..1805
                     /locus_tag="PKL11_01950"
     CDS             705..1805
                     /locus_tag="PKL11_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ12390.1"
                     /translation="MNAADLYQQIADSLRRQILEGELQPGDRLPPMREQAVRWNCTPG
                     TVQRAYQELARLGLVVSRPGQGTCVTGNRPVESDTPLQRAALVHRAEAFLLEMLTAGH
                     PLPSIQQALDLAMDRWRALQQTSEQSAPKTLQFVGSHDIAVNWLQAHFGDISPGATLN
                     VRFGGSLAGLIALAEGRADLAGAHLWDPETDTYNVPFIRKLLPGQRVALVRLASRRLG
                     WAVALGNPRGFKSFADLLRPDIRFINRQPGSGTRVWLDAQLHQYGIAAEAIQGYADTQ
                     PTHTAVARAIAEGKADVGLALEASAALYGLAFSPLTEECYDLIIPTAAYNRPEISDLL
                     TWLRPEALSTTLSSLRGYNFAQCGQVQWVEAA"
     gene            1975..2241
                     /locus_tag="PKL11_01955"
     CDS             1975..2241
                     /locus_tag="PKL11_01955"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNZ12391.1"
                     /translation="MGTLRWGDRVILHAWGQRYIYEIRWVGITKPNDSSVLDETNRSW
                     LTLITCKGYNEALNTYSSRVVAQAVLLRYEKDTTGISGPAEQQP"
     gene            complement(2286..3041)
                     /locus_tag="PKL11_01960"
     CDS             complement(2286..3041)
                     /locus_tag="PKL11_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNZ12392.1"
                     /translation="MDKFVIENLSLTYSDGAESLRGVNLTIRSNAVTVLFGPAGGGKS
                     TLLRTLNRLNDLADVAEVSGKVLFNGIDLLDPRLNVTELRRKVGIVFSRPVPLPLTIR
                     QNVSYGLEMAGERNKARLNAAVEEALRLAALWDEVYDRLDAPATAISGGQQQRLCLAR
                     VLALEPEVILLDEPTSALDPISTAKIETLLMDLTEHYTIVVAPHNVQQAARIADFGAF
                     FLQGELVEFGVRDKIFLTPQDKRTQDYIEGRFG"
     gene            complement(3048..3911)
                     /gene="pstA"
                     /locus_tag="PKL11_01965"
     CDS             complement(3048..3911)
                     /gene="pstA"
                     /locus_tag="PKL11_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNZ12393.1"
                     /translation="MKPMRLLNARQQQVIGFNLLRLVSLVTVLPILGAVIYIFVRGMP
                     AISWEFLTAVPRGGMREGGIMPAIVGTLYLTLGTAVFSVPLGVAAGIYIAEYAKDNAL
                     TRLIRIAIINLAGIPSVVYGLFGFGIFVLFLRFGTSILAASLTLSIMTLPVIISTTEE
                     ALRSVPNSFRVVSTSLGATRWQTVWRIVLPQAMPGILTGVILGLERAAGETAPILFTG
                     AAFFLPRLPGSLLDATMALPYHIFVISTQVPGMPIQIQYGTVLVLLVFVLGMNLIATL
                     IRSRARARRQW"
     gene            complement(3908..4810)
                     /gene="pstC"
                     /locus_tag="PKL11_01970"
     CDS             complement(3908..4810)
                     /gene="pstC"
                     /locus_tag="PKL11_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNZ12394.1"
                     /translation="MMENSRTRRSEFAITFFIKAAGYSSILLVTAIFIFLMKEGLPAL
                     GDVDLKTLFGSRWYPIEGYFGIWPLILGSLLVTVGAALVAIPLGVGTAVYISEVAPAW
                     IKEILKPLIEILAGLPSVVLGFLGITVAVPLLRRLLDLPTGLTAFTGAVMLGLISIPT
                     IVSIGEDALNTVPQSYRQAALALGATRWQTIWGVTLPAARSGVITAIMLGIGRALGET
                     MAVMMVTGNAPVLPIQWNALFMPVRTMTATIASEMGEVATGSAHYHVLFFIGIVLFLL
                     SLIVNIFASFVSSKQRRRAERVLS"
     gene            complement(4807..5691)
                     /locus_tag="PKL11_01975"
     CDS             complement(4807..5691)
                     /locus_tag="PKL11_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ12395.1"
                     /translation="MTKRTLILLLIGLMLLLAGCRGDSGTSGQASVSLQNKGSDTMVN
                     LALAWAERYQAEHPEASISVTGGGSGTGLSALLNGTVDIANASRAIKDEELEQAQAAG
                     FTPGEYVVARDAIAVIVNPENPVKELTLAQISAIYRCEITNWSQVGGEDRPIVRLSRE
                     TNSGTHVYFLEEVIRLGDKTNTDIFCAETLLMPSSEGIIAEVADNPNAIGYDGLGYVT
                     EDVKMLALSENPAGPFILPSVENVNAGTYPIARDLYMYTRTDTPDGLVKAYLEWVFSA
                     EAQKIVSDLGFVPVEAAP"
     gene            6026..6907
                     /gene="rpsB"
                     /locus_tag="PKL11_01980"
     CDS             6026..6907
                     /gene="rpsB"
                     /locus_tag="PKL11_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNZ12396.1"
                     /translation="MAVISMKALLESGVHFGHRTNKWNPAMRPFIFTERNGIHIIDLQ
                     QTVKSLTTAYNLVRETVSNGGVILFVGTKRQAQETVAEEARRCGMPYVTERWLGGMLT
                     NWTTIFQRIQELERLEKMRDTGELQRLTKKEGLLIERDIARLELRLGGIRTMKKLPEL
                     VFVVDVGREYTAVHEANLRNIPVIALVDTNCDPSGVDYVIPSNDDAIRAVKLLVGKIA
                     DAVIEGKAVHKEDEGTEAEAAAAEQPARRSAAPRIRMDEESELADTALLGESTLAKLA
                     PRPETTETTETTGEAAE"
     gene            6935..7540
                     /gene="tsf"
                     /locus_tag="PKL11_01985"
     CDS             6935..7540
                     /gene="tsf"
                     /locus_tag="PKL11_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNZ12397.1"
                     /translation="MAITTDMIKELREATGAGILDCRKALEQANGDMKAAVDFLREKG
                     LATAAKRSGRMAAEGVVEMYSHGNGRVGVMVEVNCETDFVGRSEAFRTFAHELALQIA
                     ASSPLYIREEDVPQDVLDHEAQIATAKAREEGKGEAVLPRIVEGALKKFKVETVLLLQ
                     PYIRDESITIQQLLNDAVVKTGENIVIRRFVRWGLGEVSGE"
     gene            7598..8317
                     /gene="pyrH"
                     /locus_tag="PKL11_01990"
     CDS             7598..8317
                     /gene="pyrH"
                     /locus_tag="PKL11_01990"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNZ12398.1"
                     /translation="MDGLKYHRILLKLSGEALASPQGFGIDPQRALHIAERVGEVREL
                     GVDVAIVIGAGNLWRGRVGLEVGMDRATADYMGMLATVMNALALMDALESRGVVTRVQ
                     SAIEMRTVAEPYIRRRAIRHLEKGRVVIFGGGTGNPYFSTDTAAALRAMEIGADVLIK
                     ATQVDGVYDSDPKRNPAAKKFDRVSHIETLNRRLEVMDSTAISLCMDNKLPILVLNLW
                     DPNALRQALLGEPVGTLVTSD"
     gene            8421..9737
                     /locus_tag="PKL11_01995"
     CDS             8421..9737
                     /locus_tag="PKL11_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNZ12399.1"
                     /translation="MKRFLGVCWLILLIAGLLTTVAQAQSQGGTALVLTAEGPLTPVL
                     VQYLDRGLGVAQSQGAELVILQLNTPGGSTDLMNQMVTLIRNSDIPVVVYVTPNGAMA
                     GSAGTIITLSAHAAYMAPETIIGAASPVDSEGGDLGETMDAKIKNVLSAQVRSYAERR
                     GPAAVALAEDTIQNARAAAASEALEVGLIDGIAVDLPDLLNQLDGQVVLVNGANQTLN
                     TRDLTPKALEQTFIEELLQLLTNPNLVFLLLSIGVQAILIELSSPGGWVAGFIGAVCL
                     ALAIYGLGILPVNWFGLIFLVIAFVLFILDLKAPTHGALTVAGVGSFIAGALVLFNSV
                     RIPGVPRISVPLVVGTGIFIAVTFSFAVGLALKAMRAPSIMGKETLVGKRGVVRVAIA
                     PRGQVRLGGENWVAELPEGETTPILPGQTVEVIAVEGLRLKVRPEK"
     gene            9734..10861
                     /locus_tag="PKL11_02000"
     CDS             9734..10861
                     /locus_tag="PKL11_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12400.1"
                     /translation="MKPRRLLRCMVLLAWLLLLSACLWRRPVQVGFAGELSGRESDLG
                     IGSRNGVLLAAEQINAAGGIDGHPIEIIVKDDAGKPDVARQVDQELVDAGVVAIIGHS
                     TTSQTIAGLEVTQPAGIVMLSPSASSQSLTGIDDLFFRVCADNTLDVIALANHIYAGG
                     QHTLAIIYDADNDAYARRYAEGVQMEFERLGGKITRIEAISADAILDAEDVLDIVLPD
                     KPEAILVVASAVNTAVIIQRAQLLDSTAAFYGSDWAYSDAFLQAGGRAIEGVELAAAF
                     DIQSPRQQMQDFKALYLERFGFAPNFTAGQAYEAMMVLAHALEQTGGSADGLPEALLD
                     IQDFEGLSSTISLNQYGDVVRPAFLFQVDDGEFVLIGVFEP"
     gene            complement(10876..11403)
                     /locus_tag="PKL11_02005"
     CDS             complement(10876..11403)
                     /locus_tag="PKL11_02005"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNZ12401.1"
                     /translation="MENILSGQAVLLALLIFGLRIVDMALDTLRILFVVRGRRKLVWV
                     CGFFQALVFVIALTTVLSNLDSILNILAYAAGFATGNVIGMYIEERLALGHIQLTIIS
                     SSRGAAIAEQLRASGFAVTELPARGRDGVVSLLTVNVIRRNVSTAETIALENDPEAFI
                     TAEEVRPMRRGFWRA"
     gene            complement(11427..13580)
                     /gene="glgP"
                     /locus_tag="PKL11_02010"
     CDS             complement(11427..13580)
                     /gene="glgP"
                     /locus_tag="PKL11_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNZ12402.1"
                     /translation="MPNYRLDIPNQFSLPRRIGRLGELAYNLWWVWNPQGQALFALVD
                     KSLWNQVNHNPVAFLKKVERTLLNAASNDKAYLEIYDQAMRDFDAYMAAKDTWYTTTF
                     PKLVNQQIAYFSFEFGLHESLPVYAGGLGILAGDHLKEASDLGMPIVGLGFIYNEGYF
                     VQKITEDGWQETSSFYLNFEEMPVIPVTDTEGKPFTVSIDLPGRQVTARLWAVQVGRI
                     KLFLMNTNLDENNQVDRQLTDRLYSNDPEVRIAQEILLGMGGVRALRALGYTPNAWHM
                     NEGHSAFLALERALEFVKQGLTFEQAAAEVRKNNIFTTHTPVPAGNDQFPLWLVDKYL
                     SQVWPELGLSRDDFMELGRQTMPWGESFVMPVLALRLSEGRNGVSDLHGQVSRKMWNF
                     LWPDRRAEAVPITHITNGVHMPSFLARRMRLLFDQYLGADWMARMDDPETWAKLDDIP
                     AEQIWAVRRHLKRKLAIYINEHSRQLWQSGRNTPAQVLASGALLETYSLTIGFARRFA
                     TYKRGYLALSDFERLLKLLNNAKMPVQIVFAGKAHPADEPGKLIIQQVYRAVKDSRTG
                     GRLVFLEDYDMNMARFLVQGVDVWMNTPRRPNEASGTSGMKAAMNGVLNFSVLDGWWR
                     EGYNGQNGWSIGEDADTPDGEQQDRQDAESLYTTLEKEIVPLYYDRSADNLPLEWIAR
                     MKESIRTLAPQFSTRRMVKEYMNALYLPALTSKKH"
     gene            13739..15073
                     /locus_tag="PKL11_02015"
     CDS             13739..15073
                     /locus_tag="PKL11_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNZ12403.1"
                     /translation="MHKKHLNQKKFIFWVIASALVLSAAMVFAIPALREPVLWRLEQL
                     RVRIRYALNPPEEAVFQPESTVDAMVQATMNALAPTPLSPLPTATPLPPQANTPLPTT
                     APTALPLGSALSGWTFFSQHGYWNYCAPANLAMSLSYYGWDGALTDIGAYVKPFDRDK
                     NVMPYELAEYVNTQTYFRALVREGGTLELARRLLANGFPILVEKGVYIKDYSGVVSWM
                     GHYQVLVGYDDATQTFTALDSYFGETDKPTLSVAYADLEKEWRPFNFILLVVYAPEQE
                     DQLRAVLADYTDSVSSYQIAASLASTEVQTLTGQAQFFAAFNYGTSLMRLQDYSGAAR
                     AYDEAFELYAALPKEDRPWRMMWYQTGPYFAYYYTGRYQDIVNLADTTISSTDQPYLE
                     ENFLWRSRAYYALGDYENAAADVTTCLQYHPDFTPCVEQANLLGVPVTQPTP"
     gene            15070..16308
                     /locus_tag="PKL11_02020"
     CDS             15070..16308
                     /locus_tag="PKL11_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNZ12404.1"
                     /translation="MIRILHFADAHIDMAAQGRHDPETGLPVRVLDFLKALDTIVDTA
                     IAEHVDLVIFAGDAYKDRTPVPTFQREWGRRMMRLSQAQIPTLLLVGNHDLSPAVGRA
                     HTLQEYETLAVPFIRVISRPEFLTPEQLWGLPLQLLAIPWVSRSALMAALGWQSAENG
                     DVYQEMEARLTVLVQDWLNQLDPALPTVMTAHASVQGAVYGGERAVMLGNDLVLPGSM
                     VRDPRLDYVALGHIHKAQNLNPGAHPPVIYPGSIERVDFGEAADEKGFVIAAVSRGKT
                     DLDHRPLKGRSFMDRSIELTSHQKVMEQCLAVLPPASALTGAMVRLVITYPRDWEAQI
                     DEAALRRHCESALEFHLVRRPQVAVSARLPGEHLMGLSPLELLALYWQNDKEVTSAQA
                     EELLSLAARVIAAGDGMEGE"
     gene            16316..17644
                     /locus_tag="PKL11_02025"
     CDS             16316..17644
                     /locus_tag="PKL11_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ12405.1"
                     /translation="MNTVIELVLIFLLLVLNGVFAMSEIAVISARKLRLQQRAEDGDR
                     GAKAALELNENPNRFLSTVQIGITLIGVLTGAVGGATLAERLAEVVAASKIPWLASAA
                     EGLSLAVVVLLTTYFSLVIGELIPKRLALSNPEKIARRVSPWMRVLARLTAPIVHLLS
                     WSTDIGLKLIGAPASEEPPVTEEEIKGLMEQGTQVGIFETAEQDMVSSVFRLGGRYVD
                     ALMTPRTEIEWIDLDEPFEEIRRQIIVSDHTRFPAGKGGLDNVQGILLARDFLTRCME
                     NSAPVDIEELLQPPLFVPDSMSALKVLQMLKDAGMHVALVIDEYGGLLGMVTLYDILK
                     AIVGAIPQAGDAAEPQAIRREDGSWLLDGLMQVDDFKELLDLDDLPDEDRLGYQTLGG
                     FVMSQLGSIPVSGEHFEWRRLRFEVMDMDGRRVDKILVIPASDSPAEKPE"
     gene            17648..20215
                     /locus_tag="PKL11_02030"
     CDS             17648..20215
                     /locus_tag="PKL11_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNZ12406.1"
                     /translation="MIPVTLKIEGFLSYHEFVSVDFTRFNLACISGANGAGKSSLLDA
                     ITWALFGKARARDDALIHSQSTQAIVEFEFDYEGNRYVVARSKKRDKTTTLELRIQTE
                     TGLYRPITEATLRLTEQRIQDILRLDYETFTNASFFLQGKADQFAQQRPAERKQILSS
                     VLGLDAWERYREQAQIRRRRSETDLAALDAQLQEIDAELAQEKDRRARLQTLEASLEQ
                     LMTARKTQESILEHLRHLAASLTEQQRLVQMLAQQAAAADQRASSLSLRLDERLADQT
                     RYQHQLADADAAYAAEAAWQAARAELERWDALAASFHQAEQRRSAPRLAIESERSRLA
                     EERRTLLRQQTDAAELDARLPNLITQMADLIVTMAENRVHLSERPALEEKLRTLIQSQ
                     SDAQAENRSLKSEMDQLKERIDRLEAASGADCPLCGQPLGPEDRQRLIAELKARGVEM
                     GARYRQNIADARTAQKTQSDLEARLEALRQLEAEQVRLDKNYVQLESSRSQMTAALTA
                     WEQGAALRLREVNDLLTAETFAPQARAELAQVDAELKTLGYDAAAHDVARRTEQSGRG
                     SQEQVRQIESARAALEPLAREIAGLQQQLAEAQSEANAQKAAHQQAAERLEQESKGVP
                     NLDDVEQAFYRLQEQENQTRMQVGGARQAVEVLTTLRERKTIKTAEREAQAYQVELLK
                     KLERTFGKDGIPARLIENALPELQEQANRILEQLSGGAMTVEFHTQRDFKDAKREDKK
                     ETLDILIRDETGAAREYELFSGGEAFRVNFAIRLALSRILAQRAGSRLQMLVIDEGFG
                     SQDAAGRARLVQAINLVRDDFACILVITHLEELKDAFPARIEVEKHPDGSQVRVLIL"
     gene            20212..21321
                     /locus_tag="PKL11_02035"
     CDS             20212..21321
                     /locus_tag="PKL11_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ12407.1"
                     /translation="MSPIPVRVGIVQRVLPAYRAAFFDALAAACTGGLSVFAGQPRSQ
                     EMIASGADLHVAHHAPADNRHLLGGAAYLCYQRGLLRWLADWQPQVLILEANPRYLSS
                     PAAVQWMKKHGSGVIGWGLGAPVLGGGVRQWLRRRFLSQFDALVAYSQSGHSQYATLG
                     LPPERIFVAPNAVAPRPAHPLPDRPAPAAPLTVLFVGRLQARKRIDLLLQACAALPES
                     LRPRLQIVGDGPARAEMEALAAQVYPSADFLGARHGAELAPIFCAADLLVLPGTGGLA
                     IQEAMSFGLPVIAAQGDGTQDDLVRPENGWRVPPGDLTALTETLTEALSNPLRLRALG
                     AESYRIVSTEINLQQMVAGFEQAIQFVLERKKCTS"
     gene            21309..22517
                     /locus_tag="PKL11_02040"
     CDS             21309..22517
                     /locus_tag="PKL11_02040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ12408.1"
                     /translation="MHILMVADGRSPIARNWITGVLSLGHRVTLVSTYPCDALPGVED
                     LVIMPVAFGGMAGGGAARRSGLRQAVGRFRRIFQRGRALLGPLLLPDSIRRFARLAAD
                     LKPDLVHALRIPFEGMLASRTPAGIPLAVSVWGNDLTLHASESRAMANLTRQTLRRAD
                     GLLADAQRDIRLAPQWGFDPARPSRVIPGGGGVDLSEIRRLAELGQILQPFNLPADAP
                     LIINPRGIRAYARADTFFQAIPLVLQRWPSVHFICPGMADQPEALQWMSALKLDERVH
                     LLPPLPQAQLWGLFLRSQATISLTVHDGTPNTLIEAMACGCLPVAGDVEALREWITPG
                     VNGLLVDPENPQSLADALVLVLNNPPLREHAAEINLDLVTRRAEMQYSRAQMQVFYLQ
                     MATQSSSPGV"
     gene            22596..23516
                     /locus_tag="PKL11_02045"
     CDS             22596..23516
                     /locus_tag="PKL11_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNZ12409.1"
                     /translation="MDNKTRDRLRGIAVGAACGDALGMALEFGAPTPVDRLIREMTPG
                     RLSAGVFTDDTETSLALAESLVNRRPLDPADLARRFLDWYRRRPEDCGLYTSMVLDGV
                     KLGLGWETAANRAQERSPLSAANGSVMRCWPVAVAYWNNRAQLWDDSILQSRVTHMHP
                     ECVAACLFINIMIFEILHGSDRQAAFETALKEVELDPELRQMIVAAPTRRREQLRNTG
                     WVRHTMESVLWGFMTTDSFEEAVVQVANLGADADSSATVVGALAGSWYGLSGIPTRWQ
                     DKLHGPWPIRSETIWRTPQFVALADQLSGA"
     gene            23611..25191
                     /locus_tag="PKL11_02050"
     CDS             23611..25191
                     /locus_tag="PKL11_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNZ12410.1"
                     /translation="MTDTFKLTYGTMFNPPEELHTRYDEALVQVKARLGQEHAMIIDG
                     QERFAAAKFEDRSPADTDVLLGIFQKGGAQDANDAIAAAKRAFPAWSHTPWQERIALV
                     RKAAALMDQRIFEMGVVVSLEVGKNRMEALGDVAETADLFRYACDRMEASNGFVTEMG
                     RDPLTGYLSTNTSVLRPYGVWLVISPFNFPAALTGGPTGAALVAGNTVVIKPATDTPW
                     TARLIAECLRDAGVPAGVFNFVTGPGSTLGQALIEHPDVAGVTFTGSLPVGMKIFRDF
                     AQGPWVRPTILELGGKNPAVVTRHADLEAAAVGILRSAFGLQGQKCSACSRVFIEAPV
                     YDELTARLAALTEKLVIGDPTDRSVYLGPVVNKSSYADYQRFVADLAQHGRILTGGHV
                     LTDGAFARGYFCAPTLAADLPYDHPLWKEEMFLPITTIGKVSSFEEAMQRANDVSYGL
                     TAGVYGSEEEVERFFDGIEAGVTYANRPQGATTGAWPGFQPFGGWKGSGSSGKNSGGV
                     HYVQLYMHEQIRTHVRKA"
     gene            25188..25517
                     /locus_tag="PKL11_02055"
     CDS             25188..25517
                     /locus_tag="PKL11_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12411.1"
                     /translation="MTTGNRVGTFLMWVGGFFLLMFVFSIIALQPALLLLVIGAPAFG
                     LGIFLWIGSPKPKSAPSGRFRVLQKKKKAPPAAPPAGSAKPPRFRVPGFFGSRHKSND
                     KDKEKNK"
     gene            25514..26416
                     /locus_tag="PKL11_02060"
     CDS             25514..26416
                     /locus_tag="PKL11_02060"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNZ12412.1"
                     /translation="MTKLCTLSEAIAQGVHDGDCVYAAGFTHLIPFAAGHEIIRQGRK
                     NLVLARATPDLIYDQMVAAGCAAKVIYSYMGNPGVGSLRIVRAALESGSVAGEEYSHF
                     GMITRLQAGATGLPFMPMNQTAATDLERNNPNYRRVTDPYTGVEVVVVPPLIPDVAII
                     HVQRCDAEGNAQIWGIVGEQKEVAFAARHVIITAEEIVDADVIRSDPNRTLIPGFIVD
                     AVCHVPHCAHPSYTQGYYDRDNVFYLEWDKISESPEAVQQWLQEWVYGLKDHTEYWQK
                     LGAETHRRLQVPEQLAAPVNYGAY"
     gene            26418..27176
                     /locus_tag="PKL11_02065"
     CDS             26418..27176
                     /locus_tag="PKL11_02065"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNZ12413.1"
                     /translation="MTYSASELMTVNAARLLRDGDVVFVGVGLPNLACNLARRTHAPD
                     LLMIYEAGVIGARPQRLPLSIGDPTLVTGALAVCSMYEIFALYLQRGNVDVGFLGGAQ
                     IDRFGNINATVIGDYAHPKVRLPGSGGSQEIAAWANRCYIITPHQKRRFPEKVDFGTS
                     AGFLGGRAQRDAAGVRGGGPQAVVTDLGVLEPDANGEMILTALHPGATVEQARANTGW
                     DLKVAPQLRTTEPPAESELRILREELDPTGIYLK"
     gene            27243..28268
                     /locus_tag="PKL11_02070"
     CDS             27243..28268
                     /locus_tag="PKL11_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNZ12414.1"
                     /translation="MTNPPPVEVLIALPLSEALQERIRALSPRLRISYHPALKVDDVD
                     DAAWRRCEVLFTDRVWPSPEQAPNLRWIQFFLSGVDFAANAPILHRPDVIATTMSGAS
                     APQVSEYILLMLLALGHKMADMAALQARAEWPRDRWERLAPFELRGSTVGFLSYGSIA
                     REAARLLQPWGVKILAVKRDVMHPQDTGYVAPGLGDPEGELFTRLYPFEAIGSMLKLC
                     DYVVNLLPLTPETRGLIGAPEFAQLKPTAYWIEVGRGGVVDQTALLNALQERRIAGAA
                     FDVFPEEPLPSNSPFWRLPNVIISPHVAGISPHYHDRAVAVFIENLQRYLAGSTLLNR
                     FDPQRGY"
     gene            28269..29000
                     /locus_tag="PKL11_02075"
     CDS             28269..29000
                     /locus_tag="PKL11_02075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ12415.1"
                     /translation="MARSFDLILFDLGNTLIYSTRHWRDLFFQSHTALCQALLAAGVP
                     IDPERFAARFRQRTAENFAAREASLVEITTAQILQETLQLEGFADVPSETIRQSLAAM
                     YAVTQQNYCLEADTHATLTALRSSGCRLGLISNAADAADVNTLIDRDNLRPYFERIWI
                     SAEVGVRKPHPHIFQLALDFFGTSLARTAMVGDSLPADVQGAQSVGITAIWLTRRADS
                     PENHAVQSHIHPDRSIPTLAALLKV"
     gene            complement(29059..30018)
                     /locus_tag="PKL11_02080"
     CDS             complement(29059..30018)
                     /locus_tag="PKL11_02080"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNZ12416.1"
                     /translation="MKKVLMMVSTVLVMALILSACNMPRGTTEPTTGPTEPAATIVAR
                     TVSALLTQSAMLTPQATATQPPVVQPTAAVPTAEVPTLAPTTPATPVLPTITPTPQIC
                     DRGSFVSETIPDDTEYQPGTSFVKTWRIKNNGTCTWTTAYSFVFVSGDAMGATTAIPL
                     PSSVAPGATVDLTVTLTAPATANTYRGDWMLQNASGVRFGLGDKADKKFWVQIKVVSA
                     PFAVTSIAMSANPSNYSGVCPVTITFTGGMTASAPGTVTYYWERSDGAKSATQSLAFT
                     AAGTQNVTYTWQLGTTYTGWVKIYVDAPNHQYFAPANFTVTCP"
     gene            complement(30129..>31448)
                     /locus_tag="PKL11_02085"
     CDS             complement(30129..>31448)
                     /locus_tag="PKL11_02085"
                     /inference="COORDINATES: protein motif:HMM:NF028231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ12417.1"
                     /translation="ERLLLPAIERDVRRYLTEMAGLHAINIFATNLRALLLQPPLAGH
                     TVLGLDPGFRTGCKVAVVDPTGKLLDTTTIYPHEPQKHLAESLETLKKLVQKYHVTLI
                     TIGNGTASRETEALAARLIDETPGVRYLIVSEAGASVYSASPLARAEMPNLDVSLRGA
                     VSIARRAQDPLAELVKIEPKAIGVGMYQHDVDQTALGQSLQGVVEDVVNRVGVDVNTA
                     SPALLTHVAGIGPKLAEKIVAHRDENGPFRNRAELRKVPGLGPKAFEQSAGFLRINGG
                     ENPLDASAIHPESYGAAQNVLTLAEITGETGTEERRARLDKLVKGQDLAALAEAVGCG
                     VPTLKDILEQLVRPGRDPRADTPAPILRTDVLKMEDLVVGMKLKGTVRNVVDFGAFVD
                     IGVKQDGLLHRTQIPTGTRLQAGQIIEVEILKVENERGRIGLGWAAG"
     assembly_gap    31449..31548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(31694..32434)
                     /locus_tag="PKL11_02090"
     CDS             complement(31694..32434)
                     /locus_tag="PKL11_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNZ12418.1"
                     /translation="MPILDDLLNSITNDAPVENVLVGAFWTMVCSRGCGMAATYKSPL
                     AHGRQTVRNVGNMLGMSARELAEYARSDDLLEASIGLAALNSLLPEPGVAEVELNAFE
                     ILAQAGENQNVALVGHFPFVDRLRPRVGQLWVLEQDPLEGDYPAEAAVDLLPQAKIIG
                     ITASTLINHTLDDLLTLCPAQAQVLLLGPSTPLSPILFQHGVTILSGTRVIDPATVLR
                     YISQGASFREVKGVRLVTMTNSSKDLTP"
     gene            complement(32445..33386)
                     /locus_tag="PKL11_02095"
     CDS             complement(32445..33386)
                     /locus_tag="PKL11_02095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ12419.1"
                     /translation="MGRFQKLLVGLMIIGLAAGCTASPAATTPPASPVPATATPEPTP
                     TPVPSTATLEATTTPPTATITPTAAPVVMNLDPEDWKSWPVMPVVPDHVREIYQLGQT
                     LGNDPHAFSILGDCQSLPETFLGVYETDPEKASQLPLELQELVTQFDGSFNRESPTVK
                     LGTTAGAIIWEEWHENLYGCKSNETPLDCELRIHKPAFVIINLGTHWESRNSMYLRKI
                     LDQLIANGVVPILSTKADNREGDDRLNLEMAQLAVEYDLPLWNFWAAVGDLPNRGLGY
                     QKGNEHIGEVYLSEEGLERHRHTAIEALNAVWRAVSR"
     gene            complement(33401..33781)
                     /locus_tag="PKL11_02100"
     CDS             complement(33401..33781)
                     /locus_tag="PKL11_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12420.1"
                     /translation="MKPITLLALICVILACAACTPEKSEAAQTIEVYLQALVDKDETR
                     LVALTCPDYEMDALLELDAFQNVVTTLEGLACEEAGTEGDYTLVNCEGQIMASYGAEQ
                     RQFDLSERTYRLLSSADGWLVCGD"
     gene            complement(33816..34424)
                     /locus_tag="PKL11_02105"
     CDS             complement(33816..34424)
                     /locus_tag="PKL11_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ12421.1"
                     /translation="MPIQHRAEETHARILQAAESCFGKAGYEAASVDMICKEAGVTKG
                     AFYHHFPSKQALFLTMLDLWLGQLDQAFVAARNSCKTVPEALALMAAQSEMIFADRPS
                     RLPLLIEFWNQATRDPTIWQATIEPYRRYHQFFAQMLREGMTEGSLRPMDADLSARVL
                     VAMAIGLILQGLMDPTGDDWGKVTRDGIAALLAGMVNPDFKP"
     gene            complement(34910..35569)
                     /locus_tag="PKL11_02110"
     CDS             complement(34910..35569)
                     /locus_tag="PKL11_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNZ12422.1"
                     /translation="MRTIPLFPLSTVLFPGMPLELHIFEPRYKLMIGRCLDEQIPFGV
                     VLIRSGQEANGPLAEVFPVGCTAEIAHVEPLSDGRMNLLVIGTERFKVLELNDEQPYL
                     AGRVETLPLEPPQTLAVLRGARQLPAMLRYYLHGLAEHEVVDLDAAQIDFPHDPLAQM
                     YLAAALLQIPSIEKQALLAAAGAPELLTALLRLYRRENALLNRIKPPASEAAAQRAWL
                     N"
     gene            complement(35629..36786)
                     /locus_tag="PKL11_02115"
     CDS             complement(35629..36786)
                     /locus_tag="PKL11_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNZ12423.1"
                     /translation="MTISPDPIETPAPVVPPMPRPAAPQRTASKWRGLIAGIGAALLV
                     ICCLGLLAIWVGSESGGSAFLVGLALAIVPVPLYTLLAVWMDRFEPEPAWMLIGAFIW
                     GGVVATAVSAILNTAGGLAVALMLGDVAGEMFSAVMSAPFVEETTKGLALLILFLWKK
                     DEFDNIVDGVLYAAMLGLGFAMIENVIYYGRAYYEEGFSGSMTLFVLRGLITSFLHPL
                     CTSMTGVGLGWARQTTRGWVKVVAPLGGYFLAMTLHGLWNLSATLGAWPIAYIFVLLP
                     TLLGTLILVWVHQSREGKIVRQYLTPEMENGLLNPGAFEEMYSLKVRGKALWKILFSE
                     GLKAWQKRMKLHQMGGELAFHRWRVARGVVKNDETAAAKAIDLQQNMLLLQ"
     gene            36780..>36974
                     /locus_tag="PKL11_02120"
     CDS             36780..>36974
                     /locus_tag="PKL11_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12424.1"
                     /translation="MSSLTSNKDKFYLIIQDARLFEVVIFQTHAIVLSSWMSVNERAA
                     FSLQASQVLPATVLAPGSLGG"
BASE COUNT         7454 a        11629 c         9850 g         7941 t
ORIGIN      
        1 cacgagatct atctctctgg cctgcagttt gcctgcgctg ccgatggcta cctttgggct
       61 ttcgatggcg ccttgctggg attgtgggat ccccgcccgg gagagcggct gacccagttt
      121 gattaccagc ccgtcattcc gccattttgg gcctatcaat ccctctggtt tgcggttgtc
      181 tccgatgccg gccccgaact ggtatcgatt gacccattca caggcgatga actctcccgc
      241 ctgccttttg ctgacctgtt tggccccgat gtggtcatca gccgcggcct tgaagccgct
      301 ggttggatct ggctgatcga caccgatgga cacttctggc tcatgtcgcc agaccaggtc
      361 tttctcgatt tcgccgggcc gtccggcgcc cgtgccctgg cttttgatga ctaccgcatc
      421 tgggtagcgg gcaccgagca aacctggcgt attgatgccg gttctgccga agtgattgat
      481 aagctggatc tgcaattaac cgccctgacc tatggcaatg gccgcatctg ggccttggat
      541 gagactggcg ccctcttgag tctcgaccct gagacggctg agcccgaatc ctttggcacg
      601 gtctgcgcct atccttcaga cctggcctgg aatgatggtc tattgtgggt aacctgccca
      661 cccgaccaat ccctcatcgc actgcaaccc tagcaggata gataatgaac gctgccgatc
      721 tttatcaaca gattgctgac agccttcgcc gccagatttt ggaaggtgag cttcagccgg
      781 gcgatcgctt gcctccaatg cgtgagcagg ccgtgcgctg gaattgcacc cccggtacag
      841 ttcagcgcgc gtatcaagaa ctggcccgcc tgggccttgt ggtcagccgc cccgggcagg
      901 gcacctgtgt taccggcaac cgcccggttg aatcagacac cccccttcaa cgggccgccc
      961 tggtccaccg tgctgaggcc tttctgctcg aaatgttgac ggcgggccat ccattgccat
     1021 ccattcagca ggcgcttgac cttgccatgg atcgctggcg cgccctgcag caaacctctg
     1081 aacaatcagc gcctaaaacc ttgcagtttg tcggcagcca cgatattgcc gtcaattggc
     1141 ttcaggcgca ttttggcgat atttcccccg gcgcgacctt gaacgttcga tttggcggta
     1201 gtttagccgg gctgattgct ctagccgaag gtcgtgccga tctggctggc gcgcacctct
     1261 gggatcccga aaccgacacc tacaatgttc ctttcattcg caagttgttg cccggtcagc
     1321 gggtggctct ggttcgcctg gcttcgcgcc ggttaggctg ggcagtggcc ttgggcaacc
     1381 cccgcggctt caagagcttt gccgatctgc tgcgtcctga tattcggttt atcaatcgcc
     1441 aacccggctc tggcacccgt gtctggctgg atgctcaact acatcaatac ggaattgccg
     1501 ctgaggcaat tcaagggtac gcagatacac agccaaccca tactgctgtt gcgcgggcca
     1561 ttgctgaggg caaggccgat gttggtttgg cgttggaggc ttctgccgca ctttacggac
     1621 tggctttttc cccgctgact gaagaatgtt atgatctcat cattcccaca gcggcttata
     1681 atcgcccaga aatttccgat cttttgacct ggctgcggcc agaagcactc tcaaccaccc
     1741 tttccagcct gcggggatat aactttgctc aatgcggcca ggttcagtgg gtagaagctg
     1801 cttgataaaa atttttggaa agggtatccc tccgcttgtg acaatctttc gcggagaaat
     1861 ccacaaaaca ttcagaaaaa ttttggggat atagttggac tctctggtta acgaagcgct
     1921 gaccataccc tttaaatcaa tcgtctggaa tgtaattggg gcccctttgc ccgcctgggt
     1981 acccttcgct ggggtgaccg tgtgatcctg catgcctggg gtcagcgcta tatctacgag
     2041 atccgctggg tgggtatcac caagcccaac gactcttctg ttctggatga aactaaccgg
     2101 agctggctga ccctgatcac ctgcaagggt tataacgaag cgctcaacac ctattcctct
     2161 cgtgttgtcg ctcaagcggt gctcctgcgt tatgagaagg atacaactgg catctccgga
     2221 ccagcggagc agcagcccta agttaatcaa aaatcgggtg ggcctttggg cccacccgat
     2281 tgattttaac caaaccggcc ttcaatataa tcttgcgtcc gtttatcctg cggcgtcaag
     2341 aagattttat ctcggacccc aaattccacc agttcaccct gcaggaagaa ggccccaaaa
     2401 tctgcaatcc gggcagcttg ctgcacgtta tgcggcgcca cgacgatggt atagtgctcg
     2461 gtcaggtcca tgagtagcgt ttcgattttc gcagttgaaa tcgggtccag tgccgaagtc
     2521 ggttcatcca gcagaatcac ttcgggttcc agcgccagca cccgcgccag gcacagccgc
     2581 tgttgctgcc cgccagaaat cgccgtagcc ggcgcatcca accggtcata aacttcatcc
     2641 cacagtgcgg ccaggcgcag cgcctcttcc acagccgcat tcaggcgcgc cttattgcgt
     2701 tccccggcca tttccagccc gtagctgaca ttctgtcgaa tcgttagcgg cagcggcacc
     2761 ggtcgcgaga aaacaatccc aaccttgcgc cgtagctcag tcacattcaa tcggggatca
     2821 aggagatcaa taccattaaa cagcaccttc ccgctgacct cagccacatc agccaggtca
     2881 ttcaggcggt tgagggtccg cagcagggtt gattttcctc cgcccgccgg cccaaataaa
     2941 accgtgaccg cgttggatcg aatggtcagg ttcacgccgc gcaggctttc agctccgtca
     3001 gagtaggtca ggctcaggtt ttcgatgaca aatttatcca tggcggttca ccattgccgc
     3061 cgggcgcgcg cccgcgagcg gatgagggtg gcaatcaagt tcatgcccag cacaaacacc
     3121 agcagcacca acacggtgcc atattgaatt tggatgggca tccctggcac ctgggtggaa
     3181 atgacaaaaa tatgatacgg cagcgccatg gtcgcatcca gcagcgaacc tggcagccgc
     3241 ggcaggaaga aagccgcccc ggtgaataga atcggtgcgg tttccccggc tgcacgctcc
     3301 aaccccagaa tcacgcctgt aaggatccct ggcattgcct ggggtagcac aatccgccag
     3361 accgtttgcc agcgggtggc ccccagtgaa gtgctgacga cgcgaaacga attgggcacc
     3421 gaacgcaggg cttcttcggt tgtgctgatg atcaccggca gggtcattat agaaagcgtc
     3481 agcgaagccg ccagaatact ggtgccaaat cgcaaaaata gcacaaagat accaaaccca
     3541 aacaggccgt aaaccacaga gggtatcccc gccagattga tgatggcaat ccggatcagc
     3601 cgggtcagtg cattatcttt ggcatattcg gcaatataga tacccgcagc cacccccaac
     3661 ggcaccgaga acaccgccgt tcccagcgtg aggtataaag tacccacaat cgcgggcata
     3721 atgcccccct cacgcatccc accccgcggc acagcggtca aaaattccca gctaatggct
     3781 ggcataccgc gcacgaagat gtagatgacc gcgcccagga tgggcagcac cgtgaccagg
     3841 ctgaccaacc gcagcaggtt gaatccgatg acctgttgtt gccgggcgtt gagcaggcgc
     3901 atgggcttca tgagagcacc cgttctgccc gcctgcgctg cttggagctg acaaacgacg
     3961 caaagatatt aacgatcagc gataacagga acagcacaat tccgatgaaa aacaaaacat
     4021 ggtaatgtgc gctgccagtt gccacttcgc ccatttccga ggcaatcgtt gccgtcatgg
     4081 ttcgaacggg catgaacagg gcgttccatt gaatgggcag cacaggcgca ttccccgtga
     4141 ccatcatgac cgccatggtc tcgccaaggg ctcgcccaat gcccagcatg atcgccgtaa
     4201 taacccccga gcgcgccgcc ggcagcgtca ccccccagat ggtttgccag cgggtggctc
     4261 ctagcgccaa cgctgcctgc cggtaagact gcggcaccgt gttcaacgca tcttcgccaa
     4321 tcgaaacaat cgtcgggatg gaaatcaatc ccagcatcac ggctccagta aaagccgtca
     4381 gcccggtggg caggtccagc agccgccgca gcagcggcac cgccaccgta atgcccagaa
     4441 aacccagcac caccgatggc agcccggcca aaatttcaat caacggcttg agaatttctt
     4501 taatccaggc cggcgctacc tcagaaatat aaactgccgt gcccaccccc agcggaatgg
     4561 caaccagcgc cgcacccacc gtcaccagca gcgagcccag gatgagcggc caaatgccaa
     4621 aataaccttc aatcgggtac caccggctcc caaagagcgt cttcaaatcc acatccccca
     4681 gagccggcag accttctttc atcaggaaga taaaaatcgc cgtaaccagc aaaatgctgg
     4741 aataccccgc cgctttgatg aaaaaggtga ttgcgaactc ggagcgtcgg gtgcggctgt
     4801 tttccatcat ggcgcagcct ccacgggcac aaatcccagg tctgatacaa ttttctgcgc
     4861 ctcagccgaa aaaacccact ccagatatgc cttcaccagc ccatcagggg tatcggttcg
     4921 ggtgtacata tagagatcac gcgcaatcgg ataggtgcct gcgttcacat tttccactga
     4981 tggcaggata aatggccccg ccggattttc cgagagcgcc agcatcttga catcctcagt
     5041 cacatacccc aggccgtcat aaccaattgc gttggggtta tcggccacct ccgcaataat
     5101 gccctccgaa gagggcatta gcagcgtttc agcacaaaaa atatcggtat tggttttatc
     5161 ccccagccgg atcacttctt ccaaaaaata aacatgggtt ccagagttgg tttcacgcga
     5221 taatcgcaca atcgggcgat cctcgccacc tacctggctc caattcgtga tttcgcagcg
     5281 gtaaatggct gaaatttgcg ccagagtcaa ttccttgacc ggattttccg ggttgacgat
     5341 caccgcaatt gcatcacgcg ccaccacata ttcccccggc gtgaagcccg ctgcctgggc
     5401 ctgctccaat tcctcatcct tgatcgcccg ggaagcattg gcaatatcca ctgtcccgtt
     5461 cagcagagcc gatagccccg tgccgctgcc gccgcccgtc accgagatgc tggcttccgg
     5521 gtgctctgcc tggtaccgct ctgcccaggc cagcgccagg ttcaccatcg tatccgaccc
     5581 cttattttgg agagatacac tcgcctgtcc gcttgtgccc gaatccccgc ggcagcccgc
     5641 cagcaaaagc atcaggccaa tcagcagtaa gatcagggtg cgctttgtca cgcccgaatt
     5701 atagcgtgtt ttttcttctc gggggcaaat ggttatctgt gaacattctc agatatttac
     5761 ttcactgcat gtttggcgga gcagataggg ttttatcccg atccggtggc atgccacata
     5821 ttgaatgaat tgcgccaccg atcccgcctg attttcctga aacccggcat cccgatctgc
     5881 ccatcctgtg ttataatcgg cccgttgcgc gctgcgcagc acatcttaca cgcctcctga
     5941 ccataccctg gcgtgccatc catagctgat ggctcagggt ggaatgaaag aggcggaagc
     6001 aaaacgcaaa aaaggagaat atctaatggc tgtcatttcg atgaaagccc tgttggaaag
     6061 tggtgtccat ttcggtcatc gcaccaacaa atggaacccc gccatgcgcc ccttcatctt
     6121 cacagagcgc aatggcatcc acatcattga ccttcagcaa acggtcaaat ccctgaccac
     6181 cgcctacaat ctggtgcgcg agaccgtcag caatggcggc gtgatcctct ttgttggcac
     6241 caagcgccag gcgcaagaaa ctgtcgccga agaagcccgc cgctgcggca tgccctatgt
     6301 gaccgaacgc tggctgggcg gcatgctcac caactggacc accatcttcc aacgcatcca
     6361 ggaactggaa cgccttgaga aaatgcgcga taccggcgaa ctgcagcgcc tgaccaaaaa
     6421 agaaggcctg ctcatcgagc gtgatattgc ccgcctcgag ctgcgcctgg gtggcatccg
     6481 caccatgaaa aagctgcccg aactcgtctt tgtggttgat gtcggccgcg aatacacggc
     6541 tgtccacgaa gccaacctgc gaaacatccc tgtgatcgcc ctggtggata ccaactgcga
     6601 tccctccggt gtggactacg tcatcccctc caacgatgat gccatccgcg cggtcaaact
     6661 cctggttggc aagattgccg atgccgtcat cgaaggcaaa gctgtccaca aggaagatga
     6721 aggcacggaa gccgaagccg ccgctgccga gcagcccgcc cgccgctccg cagccccccg
     6781 cattcgcatg gacgaggaat ccgaactggc agacaccgcc ctgctcggtg aatcgaccct
     6841 tgccaagctg gccccgcgcc ccgaaaccac cgaaacgact gaaaccaccg gcgaagcagc
     6901 cgaataacca tcagcaagag acgaggaata tagaatggct attacgaccg atatgattaa
     6961 agaactgcgc gaggcaactg gcgcgggtat cctggactgc cgcaaagccc tcgagcaggc
     7021 caatggcgat atgaaagccg ccgttgattt cctgcgtgag aagggtttgg ccaccgcagc
     7081 caaacgctct ggccgcatgg ctgccgaagg cgttgtggaa atgtactcgc atggcaatgg
     7141 ccgtgtgggt gtgatggtgg aagtcaactg cgaaaccgac tttgtcggcc gctccgaagc
     7201 cttccgcacc tttgcccacg aactggccct gcagattgcc gcttcctccc ccctctacat
     7261 tcgcgaagaa gacgttcctc aggatgtcct cgatcacgaa gcgcagattg ccactgccaa
     7321 agcccgcgaa gagggcaaag gcgaagctgt tctgccccgc atcgtagaag gcgctctcaa
     7381 gaaattcaaa gtcgagactg tcctgctcct gcagccctac attcgcgacg aatccatcac
     7441 catccagcag ctgcttaacg atgctgtggt taaaaccggc gaaaatatcg tcatccggcg
     7501 gtttgtccgc tggggtttgg gcgaagtttc cggcgagtaa atgttacaat aaaaggccaa
     7561 ccgcaaggtt ggcctttatt atgcgctagg aggtactatg gacggcttga aatatcaccg
     7621 catcttgctc aaattgagtg gcgaagcgct tgccagcccg caggggtttg gcattgatcc
     7681 ccagcgcgcg cttcatattg ccgaacgggt tggcgaagtg cgtgaactgg gcgtggatgt
     7741 tgccatcgtc attggggccg gcaacctctg gcgcggccgc gtcggattgg aagttggcat
     7801 ggaccgcgcc actgcagact acatgggcat gcttgccacc gtgatgaatg ccctggccct
     7861 catggatgct ctggagagcc ggggtgttgt gacgcgtgtc caatcagcca ttgagatgcg
     7921 caccgttgcc gagccgtata ttcgccgccg cgctattcgc cacctggaga agggccgtgt
     7981 cgtcatcttc ggcgggggaa cgggcaaccc ctacttttcc actgataccg ccgctgccct
     8041 gcgcgccatg gaaattggcg ccgatgtcct catcaaggct acccaggtag atggcgtcta
     8101 cgactcggac cccaagcgca accccgctgc gaaaaaattt gaccgggtct cccatattga
     8161 aaccttgaac cgccgcctcg aagtgatgga cagcactgcc atttcactct gcatggataa
     8221 caaactcccc atcctggtgc tcaatctctg ggaccccaac gctttgcgcc aggccctcct
     8281 gggcgaaccg gtgggcaccc tcgtcacctc ggattaatta cagtcatcac caatttcggg
     8341 gatatcagcg attaaggccg gtatcccctt ttttgtttgc ataagccgtt gtacaatatt
     8401 aaaaacatac tggaggtctc atgaagcgat tcttaggggt ttgttggctg atccttctta
     8461 ttgccggact gttgaccact gtggctcagg cgcaatcgca ggggggcact gcgctcgttt
     8521 tgaccgccga aggcccgctg acgccagttc tggttcagta tcttgaccgt gggctggggg
     8581 tggctcaatc tcaaggcgcc gaactggtga tcttgcaatt aaataccccc ggcggcagca
     8641 ctgatctgat gaatcagatg gtaaccctca tccgcaacag cgatattcca gttgtcgttt
     8701 atgtaactcc caatggcgcc atggcgggca gcgccggcac catcattacc ctctcggcgc
     8761 atgccgccta catggcaccc gaaaccatca tcggcgcagc cagcccggtg gatagtgaag
     8821 gtggcgacct tggcgaaacc atggatgcca agatcaaaaa cgttctcagc gcccaggtcc
     8881 gcagttacgc cgagcgccgc ggccccgctg ccgttgccct ggctgaagat accatccaga
     8941 acgcccgcgc cgccgccgcc agcgaggccc tggaagtcgg gctgattgac ggcattgccg
     9001 ttgatttacc cgacctgctc aatcaactgg acgggcaggt tgtgctcgtc aacggcgcaa
     9061 accagaccct aaacactcgc gatctgactc caaaagcact tgaacaaacc tttatcgaag
     9121 aactgcttca actgctcacc aatcccaacc tggtctttct gctgctgtcc attggtgtgc
     9181 aggcgattct gattgagctg tccagcccgg gcggctgggt tgctggcttt atcggggcgg
     9241 tttgtctggc cctggccatt tatggcctgg gcatcctgcc ggtcaactgg tttggcctga
     9301 tcttcctggt gattgccttt gtgttgttca ttcttgacct taaagcaccc acccatggcg
     9361 ccctcacagt tgccggggtt gggtccttta ttgccggcgc cttggtgctg ttcaattcag
     9421 tccgcattcc tggcgtcccg cgcatctcgg taccgctggt ggtgggcaca ggcatcttta
     9481 tcgccgtcac gttttccttt gcggtcggtc tggcattgaa agccatgcgc gcgccttcga
     9541 ttatgggcaa agaaactctg gtgggtaagc gcggcgttgt gcgcgtagcc attgcgccgc
     9601 gtggtcaagt ccgcctgggt ggcgaaaact gggtggccga attgccagaa ggtgaaacca
     9661 cacccatctt gcccggtcaa accgtagaag tcattgccgt agaagggctg cggctcaagg
     9721 ttaggccaga aaaatgaagc cccgccgctt gcttcgctgc atggtgctgt tggcctggct
     9781 gcttttattg tcagcctgcc tctggcgccg gccggtgcag gtggggtttg ccggtgaatt
     9841 gagcggccgc gaatcagacc ttggcattgg ctcccgcaat ggtgtcctgc tggctgcgga
     9901 gcagatcaat gctgccggtg gtattgatgg tcatcccatc gaaatcatcg tcaaagacga
     9961 tgccggaaaa ccagatgttg ctcgacaggt ggaccaggaa ctggtggatg cgggcgtggt
    10021 ggcaattatt ggacatagca ctacgtctca gacgatcgcc gggcttgaag tgacccagcc
    10081 cgccgggatt gtaatgttga gcccatcggc ctccagccaa tccctcaccg gcattgatga
    10141 ccttttcttc cgtgtctgcg ccgataacac cctggatgtc attgccctgg caaatcacat
    10201 ttacgccggg ggccagcata ccctggcaat catctatgat gctgataatg atgcttatgc
    10261 cagacgctac gctgaaggcg ttcagatgga gtttgaacgt ctcggtggaa aaattacccg
    10321 catagaggca atctcagccg atgccatcct ggacgctgaa gatgtccttg atattgttct
    10381 acccgataag cccgaagcca tcctggttgt ggcctcggcg gtcaatacgg cggtcatcat
    10441 ccagcgcgcg caactattag actccaccgc tgccttttac ggctccgatt gggcttattc
    10501 agatgctttt ctccaggccg gcgggcgcgc cattgaaggc gttgagctgg ctgccgcctt
    10561 tgatattcaa agcccgcgcc agcaaatgca ggattttaag gccctctatc tggaacgctt
    10621 tggctttgca ccaaacttta cggctggcca ggcctacgag gcgatgatgg tcttagccca
    10681 cgccctggag caaaccggcg gctctgccga tggcttgccc gaggccctgc tcgacatcca
    10741 ggattttgaa ggcctcagca gcacaatctc gttgaaccaa tacggcgacg tcgtccgccc
    10801 ggcttttttg ttccaggtgg acgacggcga atttgttttg atcggcgttt ttgaaccctg
    10861 aaccgctaag gcttgctagg ctcgccagaa gccgcgccgc atggggcgca cttcttcggc
    10921 agtaataaag gcttccgggt cgttctccag cgcaatggtt tcggcggtgc tcacgttccg
    10981 gcggatcacg ttcaccgtca acaggctgac aaccccatcc cgcccgcggg cgggcagttc
    11041 ggtcacggca aagccgctgg cccgcagttg ttcggcaatg gctgccccac gtgaactgct
    11101 gatgatggtc aattgaatgt gccccagcgc cagccgctcc tcaatataca tcccaatcac
    11161 gttgccggtg gcaaaaccag ccgcatacgc cagaatgttc agaatactgt ccaggttgct
    11221 gagtacggtg gtcagcgcaa tcacaaagac cagggcctga aagaatccgc agacccatac
    11281 cagtttccgc cgcccgcgca ccacaaatag aatgcgcagg gtatccagcg ccatatctac
    11341 aatgcgcagc ccaaagatca gcagtgccag cagcaccgcc tggcctgata agatattttc
    11401 catattacag tctccttagc cagggatcag tgctttttac tggtcaatgc tggcaggtat
    11461 aacgcattca tatattcttt caccatccgc cgggtggaga actgtggcgc cagggtgcgg
    11521 atcgattcct tcatccgggc aatccactct agcggcaggt tatctgccga gcggtcgtag
    11581 taaagcggca cgatctcttt ttccagcgtc gtgtaaaggc tttcagcatc ctgacggtcc
    11641 tgctgttcgc catccggggt atcagcatct tcgccaatgg accagccgtt ctgcccgttg
    11701 tagccttccc gccaccagcc atccagcacc gagaaattga ggacgccgtt catcgccgcc
    11761 ttcatgcccg atgtgccaga ggcttcgttg ggccgccgcg gcgtgttcat ccatacatcc
    11821 acaccctgca ccaggaagcg ggccatattc atatcgtaat cttccaggaa aaccagccgc
    11881 ccgcccgtgc gtgagtcttt gaccgcccgg taaacctgct gaatgattaa cttgccgggt
    11941 tcatccgccg ggtgagcctt gcccgcaaaa acgatctgca ccggcatctt ggcgttattg
    12001 agcagtttca acagccgctc aaaatcgctc agcgccaggt aaccgcgctt ataggttgca
    12061 aaccgccgcg caaagccaat cgtcagggag taggtctcca gcagtgcgcc gctggccagc
    12121 acctgggcgg gggtgttgcg cccgctctgc cacagttgcc gcgagtgctc gttgatatag
    12181 atcgccagct tgcgcttcaa atggcgccgc acagcccaga tctgctcagc tggaatatcg
    12241 tccagtttgg cccaggtttc cgggtcatcc atccgggcca tccagtcggc gcccagatac
    12301 tggtcaaaca acagccgcat ccgccgcgcc aggaaagacg gcatatgcac gccgttggtg
    12361 atatgtgtga tcggcaccgc ttccgctcgc cggtccggcc acaggaagtt ccacatcttg
    12421 cgagaaacct gcccgtgcag atccgacacc ccattgcggc cttctgatag ccgcagggcc
    12481 agaactggca tcacaaagct ctcgccccag ggcatcgtct gccgtcccag ttccataaaa
    12541 tcatcccggc tcaggcccag ttccggccag acctggctga ggtacttatc gaccagccat
    12601 aacgggaact ggtcgttgcc cgccggcact ggcgtgtgcg tggtgaaaat gttgttcttg
    12661 cgaacttccg ccgcggcctg ctcaaaggtc agcccctgtt tgacgaattc caaagcccgt
    12721 tccagcgcca gaaaggctga gtggccttcg ttcatgtgcc aggcattggg cgtataaccc
    12781 agcgcccgca gtgcccgcac gccgcccatg cccagcaaaa tttcctgggc aatccgcact
    12841 tctggatcat tgctgtacag ccggtcagtc agttgccggt caacctggtt gttttcatcc
    12901 agattcgtat tcatcaaaaa cagcttgatc cgcccaacct gcacagccca cagccgggct
    12961 gtcacctgtc gcccgggtag atcaatcgag acggtgaaag gcttcccttc cgtgtccgtc
    13021 accgggatca ccggcatttc ctcaaagttg aggtagaaac tgctcgtttc ttgccagccg
    13081 tcctcagtga ttttttggac gaaatagccc tcattgtaaa taaaacccag ccccacaatt
    13141 ggcatcccta gatcgctggc ttccttcaga tggtcgccag ccagaatgcc cagaccgccc
    13201 gcataaacgg gcagcgactc gtgcagccca aactcaaatg agaaataagc aatctgctga
    13261 ttgaccagct tggggaatgt cgttgtgtac caggtatctt tcgccgccat ataggcgtca
    13321 aaatctcgca tggcctggtc gtaaatctcc aggtaagcct tatcattaga agccgcgttg
    13381 agcagggttc gctctacttt tttgaggaac gctaccgggt tatgattgac ttgattccag
    13441 agtgatttat ccaccagtgc aaaaagagcc tgtccctgcg gattccagac ccaccacagg
    13501 ttataggcca attctcccag ccgcccaatc cgccgcggca gactgaattg attcggaata
    13561 tccagacgat agtttggcat gatcaaatac ctcatgtctc tctcaaaatt gtttgaccca
    13621 ttgcagatca aaccaataaa ttataccatc tcaatccacg attacctgaa tcatctgatg
    13681 atttcatctc cgccccggat tggctttcgg cctcctcgag ggatgatata atttcaacat
    13741 gcacaagaaa catctcaacc aaaagaaatt catcttctgg gtcattgcca gcgccctggt
    13801 cctgtcggcg gccatggtct ttgccatccc cgcacttcga gaacctgtcc tctggcgcct
    13861 tgagcagtta cgcgtgcgca tccgctatgc cctcaatccc cccgaagaag ccgttttcca
    13921 acctgaaagc accgttgatg ccatggtgca ggcgacgatg aatgcccttg cgcccacccc
    13981 gctttcgccg ctgcccaccg ccaccccgct gccccctcag gccaacactc ctttgccgac
    14041 caccgctccc acggcgctgc cgctgggttc cgccctctct ggctggacgt ttttctccca
    14101 gcacggctac tggaattact gcgccccggc taaccttgcc atgtccctct cctactacgg
    14161 ctgggatggc gccctgaccg acatcggtgc ctacgtcaaa ccctttgacc gcgacaaaaa
    14221 cgtcatgccc tacgaactgg ccgagtacgt caatacccag acctatttcc gcgccctggt
    14281 gcgcgagggc ggcacactgg agctggcccg ccgcctgctg gccaatggct tccccattct
    14341 ggtcgaaaag ggcgtgtaca tcaaagacta ttctggcgtg gtcagttgga tgggccatta
    14401 tcaggttctg gttggctacg atgacgccac ccagaccttc actgccctgg attcgtactt
    14461 cggcgaaacc gataagccca ccctgtccgt tgcctatgct gacctggaaa aagaatggcg
    14521 gccttttaat ttcatcctgc tggttgttta cgccccagag caggaagatc aactgcgcgc
    14581 cgttctggct gattacaccg attccgtatc ctcgtatcaa attgctgcca gcctggcctc
    14641 taccgaggtc cagaccctca ccggtcaggc gcagttcttc gccgccttca actacggcac
    14701 cagtctcatg cgcctgcagg attacagcgg cgcagcccgg gcctatgatg aggcttttga
    14761 gctttatgct gccctgccca aagaagaccg cccctggcgc atgatgtggt accaaaccgg
    14821 cccctatttt gcctactact acaccggccg ctatcaagat atcgtcaacc tggccgatac
    14881 caccatttca tcaaccgacc agccttacct ggaagaaaac ttcctctggc gctcgcgggc
    14941 ctactatgcc ctcggagatt atgagaacgc tgccgctgat gtgaccacct gcctgcaata
    15001 ccaccccgac ttcaccccct gcgtcgagca ggccaacctg cttggcgtgc cggtgactca
    15061 acccacacca tgattcgcat tcttcacttt gctgatgccc atattgatat ggccgcccag
    15121 gggcgccatg accccgaaac cggcctgccc gttcgcgtcc tggactttct taaagccctg
    15181 gacacaatcg tcgataccgc cattgccgaa catgtagacc tggtcatctt cgccggcgat
    15241 gcctacaaag accgcacccc cgtccccact tttcaaaggg aatggggccg ccgcatgatg
    15301 cgcctttctc aggcgcaaat ccccaccctg ctattggtgg gcaaccatga cctttccccg
    15361 gcggtggggc gcgcccacac cctgcaggaa tacgaaaccc tggcggttcc ctttatccgc
    15421 gtcatcagcc gccccgaatt tctcaccccc gagcagcttt ggggcctgcc cctgcagctc
    15481 cttgccatcc cctgggtctc tcgttccgca ctcatggctg cgcttggctg gcagtctgcc
    15541 gaaaatggcg atgtctatca ggagatggaa gcccgcctta ccgttctggt tcaagactgg
    15601 ctgaatcaac tcgacccggc cctgccaact gttatgaccg cccacgcctc ggtgcagggt
    15661 gcagtctatg gcggcgagcg cgctgtcatg cttggcaacg atctggtgct gcccggttca
    15721 atggtgcgcg acccccgcct ggattatgtt gccctcggcc atattcacaa agcccagaac
    15781 ctcaatcccg gtgctcaccc gccagtcatc taccccggct cgattgaacg cgttgatttt
    15841 ggcgaagccg cagatgaaaa aggtttcgtc attgccgctg tttcccgcgg taaaacggat
    15901 ttggatcatc gcccgttaaa aggccgcagc tttatggatc gctccattga attgaccagt
    15961 catcaaaagg ttatggagca gtgtctggct gtcttgcccc ccgcatcggc tctgaccggg
    16021 gcaatggtcc gcctggtcat cacctacccg cgtgattggg aagctcaaat tgacgaagcc
    16081 gcactgcgcc ggcactgcga atccgccctt gaatttcatc tggttcgccg cccacaggtt
    16141 gccgtcagtg cccgtctgcc aggcgagcat ctgatggggc tgtcgccgct ggagttgttg
    16201 gcgctctact ggcaaaatga taaagaagtg acctcggctc aggcagaaga actgctcagc
    16261 ctggctgccc gggtcattgc tgccggagac ggcatggagg gagaataatc accgcatgaa
    16321 tacggttata gagcttgtgc tgatctttct actgctggtg ctgaatggcg tttttgccat
    16381 gtcagaaatt gcagtcattt cagcccgcaa gcttcgcttg cagcagcgtg ctgaggatgg
    16441 cgaccgcggg gccaaagccg cgttggaatt aaacgaaaac cccaatcgct tcctgtccac
    16501 agtccaaatt ggcattaccc tcatcggcgt cctcaccggc gctgttggcg gcgcaaccct
    16561 ggctgaacgc cttgccgaag tggttgccgc ctcgaaaatc ccctggctcg cctcggcggc
    16621 tgaaggtctt tcgctggctg tcgtggtcct gctgaccacc tacttctcgc tcgtcattgg
    16681 cgagttgatt cccaaacgcc tggcgctctc caaccccgaa aaaattgccc gccgcgtctc
    16741 cccgtggatg cgggttttgg cccggctgac agccccaatc gtccacctgc tttcctggtc
    16801 caccgatatt ggcctcaagc tgattggcgc accggcctcc gaagagcccc ccgtcaccga
    16861 agaagaaatc aaaggcctga tggagcaggg tacccaggtg ggtatttttg aaaccgccga
    16921 acaggatatg gtctccagcg tctttcgcct gggcgggcgc tatgtcgatg ctttgatgac
    16981 cccccgcacc gaaattgaat ggattgacct ggacgaaccc tttgaagaga tccgccgcca
    17041 gattattgtc agcgaccaca cccgtttccc ggcgggcaaa ggcggcctgg ataatgtcca
    17101 gggcattctt ctcgcccgtg attttctcac ccgctgcatg gaaaattctg cgccggtaga
    17161 catcgaagaa ctgcttcagc cgccgctctt tgtccccgac agcatgtccg ccctcaaagt
    17221 tttgcaaatg ctgaaagatg ccggtatgca tgtggccctg gtcattgacg aatatggcgg
    17281 tttgctgggc atggtcacgc tgtatgatat tctcaaagcc attgtgggcg ccatccccca
    17341 ggctggcgat gccgccgagc cgcaggccat tcgccgcgag gatggctcct ggctgctgga
    17401 cggcctcatg caggtggatg atttcaagga actgctcgac ctcgatgatc ttcccgacga
    17461 agatcggctg ggctatcaaa ccctgggcgg ctttgtgatg agccaactcg gcagcatccc
    17521 ggtctctggc gagcattttg aatggcgccg cctccgcttc gaagtgatgg acatggatgg
    17581 ccgccgggtg gataaaatcc tcgtcatccc cgcctcagat agtcctgcag aaaagccgga
    17641 ataaaccatg atcccggtca cccttaaaat cgaaggtttc ctgtcttatc acgaatttgt
    17701 cagcgtggac ttcacccgct tcaatctggc ctgcatctca ggcgccaatg gcgcgggtaa
    17761 atcatcactg ctggatgcca tcacctgggc tctgtttggt aaagcgcgcg cccgtgatga
    17821 cgccctgatt cactcccaat ctacccaggc aatcgtcgaa tttgagtttg attatgaggg
    17881 caatcgttat gtggtggctc gctctaaaaa gcgcgataaa accaccaccc tcgaattgcg
    17941 catccagaca gagacaggcc tctatcgtcc cattaccgag gccaccctgc gcctgactga
    18001 gcagcgcatt caagatattc tgcgcctcga ttatgaaacc ttcaccaacg cctctttctt
    18061 cctgcagggc aaggccgatc aattcgccca gcagcgcccg gccgagcgca agcaaatctt
    18121 gagcagtgtg ctcggactgg atgcctggga gcgctatcgc gaacaggcgc aaattcgccg
    18181 ccgccggtcc gaaaccgatc tcgccgccct cgatgcccag cttcaagaga ttgatgctga
    18241 gctggcccag gaaaaagacc gccgggctcg cctgcaaacg cttgaagctt ccctggagca
    18301 gttgatgacc gcccgaaaaa cccaggaatc catcctggaa cacttgcgcc atctggcggc
    18361 ttcactcacc gagcagcagc gcctggttca aatgctggcg caacaggccg ccgcagccga
    18421 tcagcgcgca tcctcgctct cgctgcgtct ggatgagcgc ctcgctgacc agacgcgtta
    18481 tcagcaccag cttgccgatg ctgatgctgc ctacgctgcc gaagccgcct ggcaggctgc
    18541 ccgcgccgag ctggaacgct gggatgccct cgccgccagc ttccaccagg cagaacagcg
    18601 ccgctcagcg ccccgcctgg cgattgaatc agagcgttct cggctggctg aggagcgccg
    18661 caccctgctg cggcagcaga ccgacgcggc cgaactcgac gcccggcttc caaacctgat
    18721 aacccaaatg gctgatctga ttgtcaccat ggcagaaaac cgggtgcacc tgtccgagcg
    18781 cccggcgctc gaagaaaaac tgcgcaccct gattcaaagc cagtccgatg cccaggctga
    18841 aaaccgcagc ctcaaatctg aaatggacca gctcaaagag cgtattgatc gccttgaagc
    18901 tgccagcggc gccgactgcc cgctctgcgg tcagccgctg ggcccggaag accgccagcg
    18961 cctgattgcc gaactgaagg cccgcggcgt cgagatgggc gctcgctacc gtcagaacat
    19021 cgccgatgcg cgcaccgccc aaaaaaccca gagtgacctc gaagcccggc tggaggctct
    19081 gcgccagctt gaagctgagc aggtgcgtct cgataaaaac tatgtccagc ttgaatccag
    19141 ccgcagccag atgaccgccg ccctgacagc ctgggagcag ggcgcggcac tgcgcctcag
    19201 ggaggtgaat gaccttttaa ccgccgaaac ctttgccccc caggcccgcg ccgaactggc
    19261 gcaggtggat gctgaactga aaaccctggg gtatgatgct gccgcccatg atgtggcccg
    19321 ccgcaccgag cagtctgggc gcggcagcca ggagcaggtc cgccagattg aatccgcccg
    19381 tgccgccctc gaacccctgg cgcgcgagat tgccggtttg cagcagcagc ttgccgaggc
    19441 gcaatccgag gccaatgcac aaaaagccgc ccatcagcag gccgccgagc gtctggagca
    19501 ggaatccaaa ggcgtcccaa atctggatga tgttgagcag gctttttacc gcctgcaaga
    19561 gcaggaaaac cagacccgta tgcaggtcgg cggcgcccgg caggcggttg aggtgcttac
    19621 caccctgcgt gaacgcaaaa caatcaaaac cgctgaacgt gaagcccagg cctatcaggt
    19681 ggaactgctc aaaaaactgg agcgcacctt tggcaaggat ggcatcccgg cccgcctgat
    19741 tgagaacgcc ctgcccgagc tgcaggaaca ggccaaccgc atccttgaac aactgagcgg
    19801 cggcgccatg acggtcgaat ttcacaccca gcgcgatttc aaagacgcca aacgtgagga
    19861 caagaaagaa acccttgata tcctcattcg ggatgaaacc ggcgccgccc gtgaatatga
    19921 actcttctcc ggcggcgagg cttttcgcgt caactttgcc atccggttgg ccctctcgcg
    19981 cattctggcc cagcgcgccg gctcgcgcct tcaaatgctg gtgattgacg aaggctttgg
    20041 cagccaggac gccgccgggc gggcgcgcct ggtgcaggcc atcaacctcg tgcgggatga
    20101 ttttgcctgc atcctggtca tcacgcatct cgaagaactc aaagacgctt ttccagcccg
    20161 cattgaagtc gaaaaacacc ccgacgggtc gcaggtgcgg gtgctgatcc tatgagtccg
    20221 atcccggtgc gcgtgggtat tgtccagcgg gttttgcccg cttatcgggc cgcctttttt
    20281 gatgccctcg cggctgcctg caccggcggg ttgagcgttt ttgccggtca gccccgctcg
    20341 caggagatga ttgccagcgg cgccgatctg catgtggcgc accatgctcc cgctgacaat
    20401 cgccatttgc ttggcggcgc cgcctacctg tgctatcaac gcggcctgct gcgctggctg
    20461 gcagactggc agccccaggt gttgattttg gaagccaacc cgcggtattt aagcagcccg
    20521 gctgccgtcc aatggatgaa aaaacatggt tccggggtca tcggctgggg attgggcgcc
    20581 ccggtgttgg gcggcggggt gcgccagtgg ctgcggcgcc gattcttgtc gcagtttgac
    20641 gcgctggttg cgtatagtca atctgggcat tctcagtacg ctaccctggg attgccgcca
    20701 gagcgcattt ttgtcgcccc caacgcggtt gctccgcgcc ccgcccatcc actgcccgac
    20761 cggcccgcgc cggcagcccc gctgacagtg ctcttcgtcg gccgcttgca ggcccgcaaa
    20821 cgtattgacc tgctgcttca ggcttgcgcc gccctgcccg aatcgctccg cccgcgcctc
    20881 caaattgtgg gggatggccc cgcccgcgcg gagatggaag ccctggcagc gcaggtttac
    20941 ccttccgctg attttctggg cgcccgccac ggcgccgaac tggctcccat tttctgcgcc
    21001 gccgatctcc tggtgctgcc cggcaccggt ggcctcgcga ttcaggaagc catgtccttt
    21061 ggtttgccgg tgattgctgc tcagggcgac ggcactcagg acgacctggt gcgccccgaa
    21121 aatggctggc gcgttccccc cggtgatctg accgccctca cggaaaccct gaccgaagcg
    21181 ctgtcgaacc cgcttcgttt gcgcgccctg ggcgctgaat cctaccgcat tgtctcaacc
    21241 gaaatcaacc tccaacaaat ggtcgccggt ttcgagcagg ccattcaatt tgtcctggag
    21301 cgcaaaaaat gcacatcctg atggttgccg atgggcgcag ccccattgcc cgcaattgga
    21361 ttaccggcgt tctttcgctg ggccaccggg tcaccctggt atccacctat ccctgcgatg
    21421 ccctgcccgg cgtggaggat ctggtgatta tgccggtagc ttttggcggc atggctggcg
    21481 ggggcgctgc ccggcgctct ggcctccggc aagcggtcgg gcgtttccgc cgcatttttc
    21541 aacgcgggcg cgctctgctg ggtccgctgc tcctccctga ttcaatccgc cgttttgcgc
    21601 gtctggcagc cgatctgaag ccagacctgg ttcacgcctt gcgtattccc tttgaaggga
    21661 tgttggcttc acgcaccccg gccggcattc ccctggctgt ctctgtctgg ggcaacgatc
    21721 tgaccctcca cgcctccgag tcgcgcgcca tggcaaacct cacccgccag accctgcgcc
    21781 gcgccgatgg cctgctggcc gacgcccagc gggatattcg cctggcgccg cagtggggct
    21841 ttgaccccgc ccgtccctcg cgggtcatcc ccggcggggg aggggtggac ctgtccgaga
    21901 tccggcggct cgccgagtta ggccaaatcc ttcaaccctt taacctccct gccgacgccc
    21961 ccctcattat caatccgcgt ggcattcgcg cctatgcccg cgccgatact ttctttcagg
    22021 ccatcccgct ggtcttgcag cgttggccat ccgtccattt catctgcccc ggcatggccg
    22081 atcagcccga agcgctgcag tggatgtctg ctctcaagct ggatgagcgc gttcacctgc
    22141 tcccgcccct gccacaggcc cagctctggg gcctgttcct ccgttcccag gccaccatct
    22201 cattgacggt tcatgatggc acccccaata cgctgataga agctatggcc tgcggttgtc
    22261 tccccgtcgc cggtgatgtt gaggccctgc gcgaatggat cacccccggc gttaacggcc
    22321 tgctggttga ccctgagaat ccacaatctc tggccgatgc ccttgtcctg gttttgaaca
    22381 atcccccgtt gcgcgagcac gcggctgaaa tcaatctgga cctggtcacc cggcgggctg
    22441 aaatgcagta ttctcgggct caaatgcagg tcttttatct tcaaatggcc acccaatcat
    22501 catctccggg cgtttaagag tggacatttt cacccgtatc aggcataata agagcaggcg
    22561 caaccaatca tttgagtatt ttggggagtg gtgtaatgga taataaaacc agagatcgct
    22621 tgcgtggaat tgccgtcggg gccgcctgtg gtgatgccct gggcatggct ttggaatttg
    22681 gcgcaccgac tccggtagat cgtttgattc gcgaaatgac ccctggtcgg ttatccgccg
    22741 gcgttttcac agacgatacc gaaacctccc tggctctggc cgaaagcctg gtgaaccgcc
    22801 gcccgcttga ccccgccgac ctcgcccgcc gctttttgga ttggtatcgc cgccgccctg
    22861 aagactgcgg tctctacacc tccatggtat tggatggcgt caagctgggt ctgggctggg
    22921 aaaccgccgc caatcgcgcc caggagcgca gcccgctcag cgctgccaac ggctctgtga
    22981 tgcgctgttg gccggtcgcc gtggcctact ggaataaccg cgcccagctc tgggatgact
    23041 caatcttgca gagccgcgtc actcatatgc atcccgaatg cgtggcggcc tgtctcttca
    23101 ttaacattat gatttttgag atattgcacg gctctgatcg tcaggccgct tttgagaccg
    23161 ccctcaaaga agttgagctg gaccccgaac tgcgccagat gattgtggcc gcgcccaccc
    23221 gccgccgcga gcagttgcgc aacacgggct gggtgcgcca cacaatggag agcgtcctct
    23281 ggggctttat gactaccgac agctttgaag aagctgtggt tcaggtagcc aacctgggtg
    23341 ctgatgccga cagctccgcc acggttgtgg gcgcgctggc gggctcctgg tatggtctgt
    23401 ctggcatccc aacccgctgg caggataagc tgcacggtcc ctggcccatt cgttctgaaa
    23461 caatctggcg caccccgcag ttcgtggccc tcgccgacca gctttccggc gcctgatccg
    23521 ctctttttga cttcccgcct tttgtggact atacttaacc ctgtcggaca accggcaggg
    23581 taactgatat tttggcttga ggaggatttc atgacagata cattcaagtt gacctatggc
    23641 acgatgttca atccacccga agaactgcac acccgctatg atgaagctct ggtgcaggtc
    23701 aaagcccggc tgggtcagga acatgccatg atcattgacg ggcaggagcg ttttgccgcc
    23761 gcaaaatttg aagatcgctc tcccgctgat accgatgtct tactgggcat tttccagaag
    23821 ggtggcgccc aggatgccaa tgacgccatt gccgccgcca agcgcgcctt ccccgcctgg
    23881 agtcacaccc cctggcagga acggattgcc ctggttcgca aagccgccgc tttaatggat
    23941 cagcgcattt ttgagatggg tgttgtggtc agcctggaag ttggcaagaa ccgcatggaa
    24001 gccctcggtg atgtcgccga aaccgccgac ctcttccgct atgcctgcga tcgcatggaa
    24061 gccagcaatg gcttcgtcac cgaaatgggc cgcgacccgc ttaccggcta cctctccacc
    24121 aatacctctg tcttacgccc ctacggcgtc tggctggtca tcagcccctt caacttcccg
    24181 gctgccctca ctggcggccc caccggcgcc gcacttgttg ccggtaacac cgtggtcatc
    24241 aaacccgcga ccgatactcc ctggaccgcc cgcctgattg ccgaatgtct gcgtgatgct
    24301 ggcgtcccgg ccggcgtttt caatttcgtc accggccccg gcagcacact gggtcaggcc
    24361 ctcattgagc atcccgatgt cgcgggcgta acctttaccg gctctctgcc cgtcggcatg
    24421 aaaatcttcc gcgattttgc ccagggtccc tgggtgcgcc ccaccatctt ggagttgggc
    24481 ggcaaaaatc cggctgtcgt cacccggcat gccgatctcg aagccgccgc ggtgggcatt
    24541 ttgcgctcag cctttggcct gcaggggcaa aaatgctccg cttgctcgcg cgtgttcatt
    24601 gaggccccgg tttacgatga attaaccgct cgcctcgccg ccctcaccga aaaactggtg
    24661 attggcgacc ccaccgaccg ctcggtttac ctgggcccgg tggtcaacaa gagctcctac
    24721 gctgactatc agcgcttcgt tgccgatctg gcgcagcatg gccgcatcct cactggcggg
    24781 catgttctga ccgatggcgc ttttgcacgc ggctatttct gtgccccaac cctcgccgcc
    24841 gatttaccct atgaccaccc gctctggaaa gaggaaatgt tcctgcccat caccaccatt
    24901 ggcaaagtct cgtcctttga agaggctatg cagcgcgcca atgatgtctc ctacggcctc
    24961 acggcaggcg tttatggctc tgaagaggaa gtggaacgct tctttgatgg cattgaggct
    25021 ggcgtgacct acgccaaccg cccgcagggc gccaccaccg gtgcctggcc cggcttccaa
    25081 ccctttggcg gttggaaggg ctcgggctcc agcggcaaga actccggcgg cgtacattat
    25141 gtgcagctct acatgcacga acaaatccgc acgcacgttc gcaaagcatg acgactggaa
    25201 accgggtcgg tacgtttttg atgtgggtgg gcgggttctt cctgctgatg tttgtgtttt
    25261 caatcatcgc actgcagccc gccctgctcc tgctcgtcat tggcgcgccg gcttttggcc
    25321 tgggcatttt tctctggatt ggcagcccta aacctaaatc cgcccccagc gggcgttttc
    25381 gggtcttgca gaagaagaaa aaagccccac cggctgcacc tccggcaggt tcggccaaac
    25441 ctccccgctt tcgtgtcccc ggtttcttcg gttcacgtca caaatctaac gataaggaca
    25501 aagagaaaaa caaatgacca aactgtgtac cctttcagaa gccattgcgc agggtgtgca
    25561 cgacggtgac tgcgtctacg ccgctggttt cacccacctc atcccctttg ccgccgggca
    25621 tgagattatt cgccagggtc gcaagaacct ggtgctggct cgcgccaccc ccgatctcat
    25681 ctacgatcaa atggtggcgg ctggttgtgc cgccaaggtg atttactcct acatgggtaa
    25741 cccgggtgtt ggctcgctgc ggattgtccg ggccgccctg gaatcgggga gtgtggctgg
    25801 cgaagaatat tcgcactttg gcatgatcac ccgtcttcag gccggcgcta ccgggctgcc
    25861 cttcatgccc atgaaccaga ccgccgccac agacctggag cgcaacaacc ccaattaccg
    25921 ccgcgtgaca gatccctaca ctggtgttga ggtggtggtg gttccgccgc tcattcccga
    25981 tgtcgccatc atccacgttc agcgctgtga tgctgaaggc aacgctcaaa tctggggcat
    26041 tgtcggtgag caaaaagagg ttgcctttgc cgcccgccat gtcatcatca ccgccgaaga
    26101 aattgtcgat gccgacgtga ttcgctcaga tcccaaccgc accctcatcc ctggctttat
    26161 tgtggatgct gtttgccatg tgccgcactg cgcccacccg tcttacaccc agggctatta
    26221 tgaccgcgat aacgtgttct acctcgaatg ggataaaatc agcgaatcgc ccgaagccgt
    26281 tcagcagtgg ctgcaggaat gggtctatgg tttgaaagac cacaccgaat actggcagaa
    26341 actgggtgcc gaaacccacc gccgcctgca ggttcccgag cagttggctg cccccgtcaa
    26401 ttatggagcg tactaaaatg acctactcag cctctgaact gatgactgtc aatgctgccc
    26461 gcctgctgcg ggatggcgat gtcgtgttcg taggcgttgg gctgcccaat ctggcctgca
    26521 acctggcgcg ccgcacccat gcccctgacc tcctgatgat ctacgaagcc ggcgtaattg
    26581 gcgctcgccc ccagcgcctg ccgctctcca ttggtgatcc caccctggtg accggcgccc
    26641 tggctgtgtg cagcatgtat gagatcttcg cactctacct ccagcgcggc aacgtagatg
    26701 tcggtttcct cggcggcgct caaattgacc gctttggcaa catcaacgcc accgtgattg
    26761 gtgactatgc ccaccccaaa gtgcgactgc ccggttcggg cggctcccag gagatcgctg
    26821 cctgggctaa ccgctgctac atcatcaccc cgcatcagaa gcgccgcttc cccgaaaagg
    26881 tggattttgg cacctctgcc gggttcctcg gcgggcgcgc ccagcgtgat gctgccggtg
    26941 tgcgcggcgg cggccctcaa gccgtggtga ctgacctggg cgttttggag cccgacgcca
    27001 atggtgagat gatcctgacg gccctgcatc ccggcgccac cgtggagcag gcccgcgcca
    27061 acactggctg ggacctgaaa gtcgccccac aattgcggac gaccgaaccc cccgccgaat
    27121 ccgagcttcg catcctccgt gaggaactcg accccacggg gatttacctc aaataacctg
    27181 tttcattttt gccggggcgg atgtcaaaat ccgccccggc gctcaatcgg ataggagccg
    27241 ccatgacaaa ccctccccct gttgaagttt taatcgcttt gccgctttcc gaggcgctgc
    27301 aagagcgcat tcgggcctta tcgccccgcc tgcgcatcag ctaccaccct gcactgaagg
    27361 tggatgatgt ggacgatgcc gcctggcggc gctgtgaagt gctcttcact gaccgggttt
    27421 ggccctctcc cgaacaggcc cccaacctgc gttggattca attcttcctt tccggggttg
    27481 attttgctgc caatgcgccc attctgcacc gccccgatgt gattgctacc accatgagcg
    27541 gcgcctccgc cccccaggtt tcggaatata tcctgctcat gctgctggcc ctggggcaca
    27601 agatggccga tatggcagcc cttcaggccc gcgccgaatg gccgcgtgac cgttgggagc
    27661 ggctggcacc ctttgaactg cgcggcagca ctgttggctt tctcagttat ggcagcattg
    27721 cccgtgaagc cgcccgcctt ctgcagccct ggggtgtcaa aattctggct gtcaagcgtg
    27781 atgtgatgca cccacaggat accggctatg ttgcccccgg tcttggcgac ccggaaggcg
    27841 aactctttac ccgtctttac ccgtttgaag ccatcggctc catgctcaaa ctgtgcgatt
    27901 atgtcgtcaa cctcctgccg ctgacccccg agacccgcgg gctgatcggc gcgcctgagt
    27961 ttgcacagct caagcccacc gcctattgga tcgaggttgg ccgcggcggc gtggtcgatc
    28021 aaaccgcact actcaacgcc ctgcaagagc gccgcatcgc cggcgccgcc tttgatgtct
    28081 tcccggaaga gcccttgccg tcaaacagcc ccttctggcg gctgcccaac gtgattattt
    28141 ctccacacgt tgcaggcatc tccccgcatt atcatgaccg cgccgtggct gtctttattg
    28201 aaaacctgca gcgttacctt gccggatcca cgctgctcaa ccgctttgat ccccagcgag
    28261 gctattgatt ggcgcgttca tttgacctga tcctctttga tctcggcaac acactgatct
    28321 attctacccg gcactggcga gatctgttct ttcaatcgca taccgcactg tgtcaggcgc
    28381 ttctcgctgc cggcgttccc attgatccag aacgctttgc ggcgcgtttt cgccagcgca
    28441 ccgccgagaa ctttgccgcg cgtgaagcca gtctggttga gataaccaca gcccagattc
    28501 ttcaagaaac cctgcagctt gagggctttg cagatgttcc ctccgagacc atccgccagt
    28561 ctctggcagc catgtatgcc gtcacccagc aaaactactg tctggaggcc gacacccatg
    28621 ccacattaac cgccctccgc agctctggct gccgcctggg gctcatctcc aatgctgccg
    28681 atgccgcgga tgtcaacacc ctcatagacc gcgacaatct gcgcccctat tttgaacgca
    28741 tttggatttc tgccgaggtc ggcgttcgca aaccgcaccc gcatatcttt caactggccc
    28801 tggatttctt tggcacttcc ctggctcgca ccgccatggt tggcgacagc ctgccagccg
    28861 acgttcaggg cgctcaatcg gttggcatca ccgccatctg gctcacccgt cgggccgatt
    28921 cccccgaaaa ccacgccgtc caatcccaca tccatcctga tcgctccatc cccaccctgg
    28981 cggcgctgct gaaggtttaa aaaaggcgtg cagcttttgg gctgcacgcc ttttttgtat
    29041 caaagcagtg tcgggttatt acgggcaggt tactgtaaaa ttagccggtg caaaatattg
    29101 gtggttgggg gcatccacat agattttcac ccaacccgta taagtggtgc ctaactgcca
    29161 ggtgtaagtc acattctggg ttcccgcggc tgtaaatgcc agactctgcg tcgcactttt
    29221 ggcgccatcg ctgcgttccc agtaataagt cacggtaccg ggtgcgctgg ctgtcatccc
    29281 acccgtgaag gtgatggtca ccgggcagac gccgctgtaa tttgacgggt tggcgctcat
    29341 cgcaattgat gtcaccgcaa acggtgccga aacaaccttg atctgcaccc agaacttttt
    29401 atcggccttg tcgccgagac cgaaccgcac gccagaggca ttctgcagca tccagtcccc
    29461 tcggtaggtg ttggcggttg ccggtgcggt cagggtgacg gtgagatcga ccgtggcgcc
    29521 gggggcaaca ctgctcggca gcggaatcgc cgtggtggcg cccatggcat ccccactgac
    29581 aaacacaaag ctgtaagccg tggtccaggt gcaggtgccg ttattcttga tgcgccaggt
    29641 tttgacaaag ctggtgccag gctggtattc tgtatcatcc ggaatggtct cgctcacaaa
    29701 gctcccccgg tcgcaaattt gtggggtggg ggtaatggtc ggcagcacag gcgttgccgg
    29761 agtggtgggt gccagggtcg gaacctcggc tgtcggcaca gccgcagttg gctgcaccac
    29821 tggcggctgg gttgcggttg cctggggtgt cagcatggcg ctttgcgtca gcagcgccga
    29881 aaccgtccgg gcaacgatgg ttgccgccgg ttcagtcggc ccggttgtgg gctccgttgt
    29941 tccccgcggc atattgcagg cagacagaat cagtgccatg accagcacag tagaaaccat
    30001 catcaagact ttcttcatct tctcttctcc tcattccaaa aataacatca atcagtatac
    30061 ggcagcctgc cttaaaaagc aaactaccgc atagaggacg ttattgccga ttgtttggtt
    30121 cccggcggtt atcctgcagc ccagcccagg ccaatgcgcc cgcgctcgtt ctccactttc
    30181 agaatctcga cctcgataat ctgcccggct tgcagccgcg taccggtggg gatctgtgtg
    30241 cggtgcagca gcccgtcctg tttgacgcca atatccacaa acgcgccaaa atcaaccacg
    30301 ttgcgcaccg ttcctttgag cttcatccca accaccaggt cttccatttt cagcacatcg
    30361 gtgcgcaaaa tcggtgccgg cgtatccgcg cgtggatcgc gcccggggcg caccagttgc
    30421 tccagaatat ccttcaaagt tggcacaccg cagcccaccg cctcagccag cgcagccagg
    30481 tcttgcccct tgaccagttt atccagccgt gcccggcgct cttcggtgcc tgtctccccg
    30541 gtgatctctg ccagcgtcag cacattttgc gccgccccat aactctctgg atggatggct
    30601 gaagcatcca acggattctc gccgccattg atgcgtaaaa aacctgccga ctgttcaaag
    30661 gctttgggcc ccagtcccgg caccttacgc agttcagccc ggttgcggaa aggtccattc
    30721 tcatcccggt gcgccacgat cttttcggcc agcttgggcc caattcctgc cacatgcgtc
    30781 agcagcgccg gcgaggccgt gttcacatcc acgcctaccc ggttcaccac atcttccacc
    30841 acaccctgca gtgattgccc cagggcagtt tgatccacat catgttgata catccccacg
    30901 ccaatcgcct tgggctcaat ctttaccagt tctgccagcg gatcttgtgc ccgtcgcgca
    30961 attgaaactg ccccgcgcag gctcacatcc aggttaggca tctcagcgcg cgccagcggg
    31021 ctggctgaat acacgctcgc cccggcttcg ctgacgatca ggtaacgtac gcccggcgtt
    31081 tcatcaatca aacgcgccgc cagggcttct gtctcacgag aagctgtgcc gttaccaatt
    31141 gtaatcagcg taacatgata tttttgcacc agcttcttca acgtttccag cgactcggcc
    31201 aaatgcttct ggggttcgtg cgggtaaatt gtcgtggtat ccagcagctt gccggtgggg
    31261 tccaccacgg ccactttgca gcccgtgcgg aaacccgggt ccagccccag aaccgtatgc
    31321 cctgccagcg gcggctgcag cagcagcgcc cgcagattgg tcgcaaaaat gttaatcgcg
    31381 tgcagaccgg ccatttcagt caaatagcgc cgcacatccc gctcaatggc gggcagcaag
    31441 agccgctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ctcttcatcc
    31561 aggctgccgg tggcttcctt gcggtaccgg gcaataaatg ggatggtatt ccccgcatcc
    31621 agcagttcga cagtcgccgc cacctggccc gggcgtacgt ttaacagcgt tgcaattttg
    31681 gtttcgtaag ttgtcatggg gtcaagtcct ttgaagaatt cgtcattgtg accaggcgta
    31741 cgccttttac ctcgcgaaaa gatgcgccct ggctgatata ccgcagtact gtcgcagggt
    31801 ctatcacccg cgttccagat aagatggtca cgccgtgctg gaataagatc ggtgacagcg
    31861 gcgtgctggg ccccagcagt aagacctgtg cctgggccgg gcagagcgtc agcagatcat
    31921 ccagcgtatg attaatcagc gtgctggctg taataccaat gattttggcc tggggcagca
    31981 aatctacagc cgcttcggcc gggtaatccc cttccaacgg gtcctgttcc agtacccaca
    32041 actgccccac ccgggggcgc agccggtcca caaatgggaa atgccccacc agggccacat
    32101 tttgattctc ccctgcctgt gccagaatct caaacgcatt gagctccact tccgccacac
    32161 cgggctcggg cagcagcgaa ttgagcgccg ccagaccaat gctggcctcc agcaggtcat
    32221 ccgaccgggc atattcggcc agttcccggg ccgacatgcc gagcatattt cccacattgc
    32281 gcaccgtttg ccgcccatgt gccagcgggc ttttgtaggt ggctgccatg ccgcagccgc
    32341 ggctgcatac catggtccag aaagcgccta ccagcacatt ttcaaccggc gcatcatttg
    32401 tgatgctgtt taatagatca tcaaggatgg gcataatcgg cttcttaccg gctcaccgcg
    32461 cgccagacgg cgttgagggc ctcaatcgcc gtgtgacggt gccgctccag gccttcctcg
    32521 ctcaaataaa cttcgccaat atgctcattc cccttctgat accccagccc gcggttgggc
    32581 agatcgccta ccgcagccca gaaattccac agcggcaggt cgtactcaac cgccaactgc
    32641 gccatttcca ggttcaggcg gtcgtcccct tcgcggttat ccgcttttgt cgagagaatc
    32701 ggtaccacgc cgttcgcaat caactgatcc aaaatcttgc gcaggtacat cgagttacgc
    32761 gattcccagt gcgttcccag gttgataatc acaaaagcgg gtttatgtat ccgcagctcg
    32821 cagtccagcg gcgtctcatt tgatttgcaa ccgtacagat tctcgtgcca ttcctcccag
    32881 attatcgccc ccgcagtcgt ccccagcttg acagtcggtg attctcgatt gaaagaaccg
    32941 tcaaattggg ttaccagttc ctgtagttcg agcggcagtt ggctggcctt ctccggatcg
    33001 gtctcgtaaa ctcccaaaaa agtctccggc aggctctggc agtcgcccag aattgaaaaa
    33061 gcatggggat cattccccag tgtttgtccc aactggtaga tttcgcgcac atggtcgggc
    33121 acaaccggca ttaccggcca gctcttccaa tcttctggat ccaggttcat gacgacgggc
    33181 gcagccgtgg gggtgatggt tgcggtaggc ggcgtggttg tggcttcaag ggtggctgtg
    33241 ctgggcaccg gtgtgggggt gggctctggc gttgccgttg ccggcaccgg ggaggccggg
    33301 ggtgttgtgg ctgccgggct tgctgtgcag cccgccgcca gcccaataat cattaatcca
    33361 accagtaatt tttgaaatct acccataaaa acctgcctga ctaatcgccg cacaccagcc
    33421 agccatccgc tgaggacagc agccggtacg ttcgctcgct caaatcaaac tggcgctgtt
    33481 ctgcgccata agaggccata atttggcctt cacagttcac cagggtatag tcgccttctg
    33541 tgccagcttc ttcacaggcc aacccctcca gggtagtaac cacgttctgg aacgcatcca
    33601 gttccagcag ggcatccatt tcataatccg ggcaggtcaa agcaaccagc cgggtttcgt
    33661 ctttgtccac cagcgcctgc aaatacactt ctatggtctg ggccgcctcc gatttctccg
    33721 gagtgcaggc tgcacaggcc aatatcacac agatcaaggc caacagtgta atcggtttca
    33781 taggttcctt ctgtcgataa cagcccggaa ttgtatcacg gtttgaagtc tggattgacc
    33841 attcctgcca acaaagccgc aatcccgtcc cgagtcacct tcccccagtc gtccccggtc
    33901 gggtccatca aaccctgcaa gatcagtcca atcgccattg ccaccaggac gcgcgccgat
    33961 aggtctgcat ccatgggcct caggctgcct tcggtcatcc cttctcgcag catttgcgca
    34021 aagaactgat gatagcgtcg atacggttca atcgttgcct gccagatggt tgggtcgcgg
    34081 gtggcctgat tccaaaactc aatcagcagc ggcagccggc tggggcggtc tgcaaaaatc
    34141 atctcactct gggctgccat cagtgccagc gcctcgggca cggtcttgca gctgttgcgc
    34201 gccgccacaa aagcctgatc caactgcccc agccacaaat ccagcatggt cagaaacagc
    34261 gcctgcttgc tcggaaaatg atgataaaaa gcccccttgg tcactccggc ttctttacaa
    34321 atcatatcca cactggcggc ctcatacccg gccttgccaa agcagctttc tgccgcctgc
    34381 aaaattcggg catgggtttc ttcggcgcgg tgttgaatcg gcatggcgca atcctccttg
    34441 gactatcggt attatagcag agagccagat gcttaaacga aacccattta cccctataaa
    34501 ttttaaactt taaaattttc caaacctatt gactccggcc tcgaatctcc ctatactgaa
    34561 tgtgcaggga gctccgagaa attggaaatc ccccaccgaa ggaagaccgg cgcagcacat
    34621 gggaatagcc tgtgaggaac ttgctcaagg ttcccatccc ctccgccggg aaatcgtctc
    34681 tcacagcggt gaaactttac aatcgaaatt cgacaatact cgataaacct gaaataagga
    34741 ccgaggtaat ttcgatagtc ataaagtgga gctgagagag aatctgacgg gctgagatag
    34801 gcctcttgca agtccagtta gttcggcagc gagagtaagt tttttcagag gcctaaagag
    34861 attaagcggt gggcgagatg gttgggttac ttggagcccc tgcttttatt taatttaacc
    34921 aggctcgctg cgccgccgcc tcgcttgctg gcggctttat tctatttaac agggcatttt
    34981 cccgccggta taaccgcagc agcgccgtca acagctctgg cgccccggca gcagccagca
    35041 aagcctgctt ttcgatggat gggatctgca gcagcgccgc cgctaaatac atctgcgcca
    35101 gcgggtcgtg cggaaaatca atctgagctg catctagatc gaccacctca tgctctgcca
    35161 gcccatgcag ataatatcgc agcattgccg gaagctgccg cgccccccgt agcactgcca
    35221 gcgtctgcgg tggttccagc ggcagggttt ccaccctgcc ggccaaatat ggctgttcgt
    35281 catttaattc taaaactttg aaccgctctg ttccaatcac cagcaggttc atccgcccgt
    35341 cagacagcgg ctccacatgc gcaatttctg ccgtacatcc caccggaaac acctctgcca
    35401 gcggcccatt ggcctcttgc ccgctgcgga tcaacaccac gccaaacgga atttgctcat
    35461 ccaggcagcg tccaatcatc agcttatacc gcggctcaaa aatgtgcagt tccaggggca
    35521 tgcctggaaa aagcaccgta ctcagtggaa aaagaggaat cgtgcgcatg aggttatgcc
    35581 cacgattctc tcagattaca gggtaaaatg ccagtaaatt tacaaggttt attgcagcaa
    35641 tagcatattt tgctgcaggt caattgcttt tgccgcagcc gtttcatcat ttttgaccac
    35701 tccgcgcgcc acacgccatc gatggaaagc cagctcgccg cccatctggt gcagcttcat
    35761 acgcttttgc caggccttaa gcccttctga gaacaaaatc ttccagagcg ccttgccgcg
    35821 cactttcagg ctgtacattt cttcaaaagc gcccggattc aacaggccgt tttccatttc
    35881 aggcgtcagg tactggcgga cgatcttgcc ttcccggctc tggtgcaccc agaccagaat
    35941 cagcgttccc agcagcgtgg gcagcagcac aaagatatag gcaatcggcc aggcacccag
    36001 cgtcgccgat aaattccaca gcccgtgcag tgtcatcgcc aggaagtacc cccccagtgg
    36061 agcgaccact ttgacccagc cgcgggtcgt ctgacgggcc cagcccaggc ccacgccggt
    36121 catcgaggtg cagagcggat gcaggaagga agtgatcagc ccgcgcagca caaagagcgt
    36181 catcgagccg ctaaagcctt cctcataata agcccgcccg tagtagatga cgttctcaat
    36241 catcgcaaag cccaggccca gcatcgcggc atataaaacg ccgtccacaa tattatcaaa
    36301 ttcatccttc ttccagagga acaggatcag cagcgccagc cctttggtcg tctcttccac
    36361 aaacggagct gacattaccg cgctgaacat ttcgcccgcc acatctccca gcatcaacgc
    36421 cactgccagc ccgccagccg tgttcaaaat cgccgagacg gcggttgcca ccacaccgcc
    36481 ccagatgaat gccccaatca gcatccaggc gggctcgggc tcaaaccgat ccatccatac
    36541 cgccagcagg gtgtacagcg gtaccggcac aatcgccagc gccaatccaa ccaaaaaggc
    36601 cgatccgcct gattcgctgc ccacccagat agccagcaaa cccaggcagc aaatcaccag
    36661 cagcgccgcg ccaatcccgg caatcagccc gcgccacttg ctggcggtgc gctgtggtgc
    36721 cgctggcctt ggcatcggcg gaacaacggg ggcaggggtt tcaatggggt caggagaaat
    36781 tgtcatcatt aacctcaaat aaggacaaat tttacttgat tatacaagat gcccgccttt
    36841 ttgaggtagt aattttccaa actcatgcta tagtattatc atcatggatg agcgtaaatg
    36901 aacgggctgc attttcgctt caggcatcac aggttttacc ggcaacagtt ctggctcctg
    36961 gcagtttggg tggt
//
LOCUS       DAOPVS010000010        35904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1124_length_35904_cov_51.784485, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000010 DAOPVS010000000
VERSION     DAOPVS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35904
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_35904_cov_51.784485"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PKL11_02125"
     CDS             <1..258
                     /locus_tag="PKL11_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNZ12425.1"
                     /translation="WGETPRTYPVDIEIKAYDRQGLMAEISTILDNEGVNVRDIQMHI
                     AHNLATIRIMVDVRDIAHLSRVLTRLENIPNVMEAHRTRPG"
     gene            391..627
                     /locus_tag="PKL11_02130"
     CDS             391..627
                     /locus_tag="PKL11_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12426.1"
                     /translation="MPKVRCHYLDCGFLDEGVCTAAMVEFDPDSGCMTYSANAGALNG
                     DDWEEEDELEEWEELEDEEEDDELWLDEDEETEA"
     gene            624..1880
                     /locus_tag="PKL11_02135"
     CDS             624..1880
                     /locus_tag="PKL11_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12427.1"
                     /translation="MRDTPIEQKLRHNYLVNLADGGFFGFAIGASSFSAILPLFVARF
                     TDSAVLIGLITAIHNVGWQLPQLFLARWIATLTRFKPWVLKLTIHERVPFLGLALIAG
                     LIFTQKPVLSLILTFVMLAWQGLGSGFAANVWQNMIGKIIPGSIRATFFGLQSSASNL
                     GGSIGAVISGILLDRLPSPLDFVLCFLITVGLMGISWFFLALTREEVTPPPTAEDVPQ
                     PTAELSRHILRSDASFKWFLVARNLFQFGTMAFSFYIIYAVLKLGMSEVEAGLMAAAL
                     NITLVIANPVLGWLADHWNIQAVMTLGAVASLLSALVAFLAPSLGWFYLVIVLQGFAN
                     VTFWTIGIAFTLQFGTDRQRPVYIGLANTLIAPSTILAPLVGGWLADAINYQATFLVS
                     AGLSGLTALIFLLFVKNPPVRKAEAG"
     gene            complement(1877..2275)
                     /locus_tag="PKL11_02140"
     CDS             complement(1877..2275)
                     /locus_tag="PKL11_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12428.1"
                     /translation="MARVIDPQTLPVDELPGVWSPVQWRLTPEERVQEMEAQASASLL
                     ANIDVPEALLRLLLNDANIERAYEPPQGYDPEQQGEWDPDLITFAFSTPIELVDVVRE
                     PDHLTAVYRFANHGMWSIEISPERVVIERV"
     gene            2350..3324
                     /locus_tag="PKL11_02145"
     CDS             2350..3324
                     /locus_tag="PKL11_02145"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNZ12429.1"
                     /translation="MSILPDSRDCFLVGKIAVKGELILAPMDGYSDLPFRSLARRLGS
                     AMSYTEFISAIDVIHGRPGLERRLAYLEEERPVVVQLLDNDPERLLAAAQKLRSLNPD
                     VIDINLGCSAKPVAGRGAGAGLLKEPLKIAQIFAMLNQTLDIPVSGKIRLGWDDQSRN
                     YLQVAQIIEENGGSLIAVHGRTKTQAYTGNADWDAIAEIKQRVSIPVIANGDVQTVAD
                     IDRIKAHTGCDGVMIGRAAVANPWIFARKDREEITIPQVRGIIEEHLDASLRFYGAEA
                     GLVFFRKYASRYMESYGISREQRQRLMTRPTSVEFLDTLDNIFKAMLE"
     gene            3370..4866
                     /gene="glpK"
                     /locus_tag="PKL11_02150"
     CDS             3370..4866
                     /gene="glpK"
                     /locus_tag="PKL11_02150"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNZ12430.1"
                     /translation="MTKFIAAIDQGTTSTRCIVFDHSGNVVAMDQQEHTQIYPKPGWV
                     EHDPLEIWFRTGEVLQNALRKSALTSEDIAAIGVTNQRETTVVWDRTTGMPYYNAIVW
                     QDTRTDTICNRLAADGGQDRFRGKVGLPLATYFSGPKIKWLLDNVAGLRAAAESGRAI
                     FGNMDTWLIWNLTGGTRGGVHVTDVTNASRTMLMNLHTLDWDDEMLAVLEIPRKMLPE
                     IRPSSAIYDYASGVLPSVPVSGDLGDQQAALFGQTCFSPGEAKNTYGTGCFMLLNTGQ
                     QPIYSQNGLLTTLGYKIGNHPAIYCLEGSIAIAGALVQWLRDNLKIIEKSGDIEPLAR
                     TVTDSGGIYIVPAFSGLYAPYWRSDARGVIVGLTRFINRGHIARAALEATAYQTREVL
                     DAMEQDSGVKLSALKVDGGMVVNDLLMQFQADILNVPVIRPRVAETTALGAAYAAGLA
                     VGYWKTVDDLRVNWGIDREWKPGIEEAQRSVQYARWKKAVARTLDWVE"
     gene            4920..4991
                     /locus_tag="PKL11_02155"
     tRNA            4920..4991
                     /locus_tag="PKL11_02155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4952..4954,aa:Gly,seq:ccc)
     gene            5102..5479
                     /locus_tag="PKL11_02160"
     CDS             5102..5479
                     /locus_tag="PKL11_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12431.1"
                     /translation="MSHKILYVEDNPDNRMLVRRLLMSCGYEITEASSAREALEALKT
                     LVPDLILMDINMPDMDGYTLTAIIKENKALEKVPVIALTANVMRGDRERTLSAGCDGY
                     IEKPIDIDRFFDQIEAFLAMYQR"
     gene            5486..5917
                     /locus_tag="PKL11_02165"
     CDS             5486..5917
                     /locus_tag="PKL11_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12432.1"
                     /translation="MPNDDSSSVQGIPKGTTVLVVEDNVSNFVLIARLLGFLDIHCEW
                     KTSGYEVVEFADTLPRIDLILMDIRLPYEDGYGATKKIRQSPKLKNVPIVAVTAEASV
                     EQMNKARNSGFDGFLSKPLDPDRFPEQISRILAGEAVWEWE"
     gene            complement(6009..6275)
                     /locus_tag="PKL11_02170"
     CDS             complement(6009..6275)
                     /locus_tag="PKL11_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12433.1"
                     /translation="MKQQFWPQNAHIHLRPDPSKYSDSQQKHHPILCPPTKTAVLQSL
                     IQAEQQSFAQRSEIGRRSGFLAVVGFVKVWLPQWLQQAAMQGSK"
     gene            6359..10252
                     /locus_tag="PKL11_02175"
     CDS             6359..10252
                     /locus_tag="PKL11_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12434.1"
                     /translation="MFATRESWESNTEALDPAVRRLLGTNDSALVYDVPPFYPNQFVL
                     ISAKVFVSNDIDQIVTMMSFSLPKDPQELLNTAKIYFPNAIGYYYTADKTLVYIDETG
                     KLAEIGLTESQIDDMQTLQSLSSQKKIQETTGLDAQPAYAYVQPVGGLKTSLVLEMPR
                     KILDAQIQSLARVNLLLIGISVLIIGALVYLGTTSIVRPLVRLANNAKSFAGGDWSER
                     ARVNRNDEIGLLANSFNEMVEQLSVLYRSMELKVEERTSQLRTASEVAQVATSVTSQA
                     EMAEKAASLIKDRFGYYYVAVYLMDATASYAILRSVACDDAEFRDLVGYRIPVNSQTM
                     IGWVASHGQSQVSLDISPTEAIEQGRNIMPLSRSAMAVPIVVGNQVLGIVEVQSTKPN
                     DFDPDAISVFQTMADQIATGFRNVSLLESAQVNLDEVGLLYRSSRQIAQAKDEREVLE
                     NLSEALSHSQYVSIVLEAAEDHLVIRSIHDPKGTGFDSTLRGVALPVRQGLGRLSETP
                     ILLIDDLRTSSEFENLLTFLARRECRTAALIPVLMDGKPNKVIAIGSREGTALSANVL
                     QPYSSIAEVAGSTLGRLNLVRSLSARLSDIESIADFSQAISTETDLSRLYKVLHDQAI
                     KTFGGDIFFLVALFDSKHDMIEVPYFYQGDRFVQMEPYPVGDGLVSHIIRTKQPLMLV
                     KNIEQTMVQMRAIHAGGVAKSWLGVPLIVGGDLVGVLSVQDIVNEERFDEGDLNLLLT
                     LAPQIANAIRNAQLLKQTQDNLQAYEAERALLNILLANTPEQIYIKNTAGEYQRVSQS
                     FADWVGISRPDDIAGKTDADLFGTEFGEKVFEEERTLLSSGINRIGEIEQTKDKAGNL
                     FWGTHSRILISDSAGLTTGILGIRQDITEIKIAEQLSMRRADQLQTIADIAREATGTL
                     DVNELLKKAVNLIRDRFGFYHSSIFLIDPNGEYAELRESTGEAGAKMMAAGHRLVVGS
                     ASMVGQSTRRGEPVVSNDVTAEPNYYPNPMLPETRSELAIPLRSGGRILGALDVQSTQ
                     TQAFSPEDIDTLQLLADQIAIAVTNSNLFARSQDALIRQRLVNEITVTAAAAQSLDEA
                     IATAVKGLREAMGGDRISVFLVDEGQRQMHLHTSAGYGEGEMETLIVPFGEGIVGEAA
                     ETAETIRVDDAQNDPRFIAMEEDIRSELAIPIHFTDQLIGVLNLESKRLAAYDESDEQ
                     ILEALGNNLGAVISNAQLLRQIRMQVERQQQLYEITSKIRRSSDLQTILRTSATEIAR
                     ALGAERAEVRLSTRTIGRAADEPTADNGHEPEIEK"
     gene            10340..12343
                     /locus_tag="PKL11_02180"
     CDS             10340..12343
                     /locus_tag="PKL11_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNZ12435.1"
                     /translation="MPILIGVVMASLRFTESTVTAQTEEQLVYVSQGVSNLLDNWLSL
                     NNNQLKNLALQPGIISMDPAQQKPILEAMAAANPYMYLVSTTNKIGMNVARSDNNELI
                     NYLDRAWFKGAINDYEFTYQILIGRTGGAPALVMSTPIKQDGAIIGVAMFAAELNQIT
                     DTVSSIKVGESGFAFVVDPYNRVMAHPNPEYVANLLDLSEYPPVVALRGGESGIYNFT
                     DAEGRNWVSHLTKTADDWGIIVQLPQSEYLALVNRQRLTAIIILAAGVVLVLSLTSTI
                     LRQVIRPVKVLTRTAVAISRGDLNRTAPVESDDEIGTLAMTFNKMTAQLRQTVDDLQI
                     RVDERTAELGNALSQLRTAADVSRSITRVMDVQTLLQEVVDLIKDRFNLYYVGVFLLD
                     ETGEQAILRAGTGEAGTKMVAAGHRLLRGGASMIGWAISKGEARISQEVGTETVRFKN
                     PLLPLTRSEVAIPILSRDKALGALTIQSTEANAFDQDDIVVLQGVADSLAAALDNAAL
                     FNQTQESLDEIRALNRAYTEEAWGRTLATYGTISAEHVNPAGVHGDQPAHQVRVPLML
                     RENPIGEVVLDMSGRDLTADELEFVEAITSQAALALESARLLEESQAYALQERKMSEM
                     TSRFSRASNIDEILKAAVTELGNLPTVAEVAIHLTPPEQAEGA"
     gene            12355..14094
                     /locus_tag="PKL11_02185"
     CDS             12355..14094
                     /locus_tag="PKL11_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12436.1"
                     /translation="MNNAGTPQQTRPQGTTEHSDILRLLQERILRNILLGAVIFGTLI
                     FIYASYVAVSRQLWGLLIFYAVTYAILLLAALVRRLPYAIRSTAIMIVVYLQGTVSML
                     GSTHPGSGMIYLLTLTILGAILFNLRVGLLMMGASVLTLIGIAIGVNSGVVPISELSL
                     LVGTSNQTIPWITYILLFVLLGTVAVSSVGSTTNGLNVSLTNQRHLTRALAQERNTLE
                     NRIQDRTTELEQRAAQLTAASEVARQMSAETDLDQLLKNAVQLIKDKFGLYYTAVFLS
                     DEKEEFAVLRSGTGEAGQAMLEAGHRLKIGEEGIVGNVVYRGEPRIALNVGADATHFK
                     NPYLPQTQSEAALPMRVGSRVIGALDVQSEREQAFGDEDIRVLQTIADQLAVAIEKTS
                     LVEQLEESLLKLETRSRETTLKTWRSHIRSSKRPFAYRYHRATAEIEPAPPISETAQA
                     VLKAGTPIIRSIPAEENQPGTTMVSVPIKMRDQVLGVLDVRLDTPTASQELINYVQMS
                     VDRLGVALDNARLLEEIQNRAEREHLVSEITGQLRKSSEVNTILSTAAVELGRALGLS
                     EVMVELRQTDADI"
     gene            14116..17019
                     /locus_tag="PKL11_02190"
     CDS             14116..17019
                     /locus_tag="PKL11_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12437.1"
                     /translation="MNQSEPQNTTSTTQAGPAENQMGLDRRIGLYTGIGAGLIGLLFL
                     SQGVTHPVIQLYIVAAVFMLVALVNIFSLESITFKDPRTSVYLGLSALQLALVVFAHL
                     FTNTSLISASLIIIIGVFTISSQFRGRDVDLGLIITLVAAMLSGLAGTLTTIGQYDLN
                     NFPLFMRIMLIVIVVAYIVVLGIIAIRSLTSTITVKLITSSLAITLIPLLLLSTIQTD
                     FIRTIVQNQTNQALKLAGEETANRIDAFFSDNLDQIERASNLAAFINYLNIDPTERNG
                     SSQEQQLALTINSLKAQQEAYLTSIGLLDLQGNNIYDSNPFEVGNNEDTSEYFIQPLR
                     TGQIFVSSVLFSDQNGDAYLYFSAPVRNASGQMMGVLRMRYDALVLQDRLGESINLLG
                     ARSYPMLVDELNIRIADLARPSEVYKAIAPLTADQIASIQKQKRLPNLPAEQLYTNLP
                     EFAASLDQIGNNPYFNTYLYESGTEARGMSGVAIRLKNKPWNVVFLQERTTLINLLQE
                     RNRTSSLIAILLAGFVGLITVIFARLFSRPILSLRETAANIAAGDINAQATVTTRDEI
                     GALGETFNLMTQQLRGFIGELENRVNERTVELARQNESLQLRSQQLQTVSDVARQVVS
                     ARELETLLNQVTVLISERFNFYHVGIFLIDQNNEYALLRAANSEGGQRMLARGHKLRV
                     GEVGMVGYVTGAGQPRIATDVGKDAVFFNNPDLPETRSEMALPLKVGDEVIGALDVQS
                     IVSNAFTAEDISLFSTLADQVAIAIRNNQLYSETMRALQESQKLHQQYLQQEWGREAG
                     ERGDVAYIYTPGGMVVRPYINTPEVERVFETRQPVIKQAEATGDRSVMAIPVMLRDEV
                     IGIIHLQEAENTVREWSNEEVATVQSVADQVGQALENARLFERTVRRAERERKVLDIT
                     SRIRASNDPQQMVQIALEELQRALRVSRAQVLVQTPPSLSEDQEDSDGESPAE"
     gene            17041..17826
                     /locus_tag="PKL11_02195"
     CDS             17041..17826
                     /locus_tag="PKL11_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNZ12438.1"
                     /translation="MSYIIAVANEKGGVAKTTSSISLGAALVESGAKVLLVDLDSQAN
                     LTLALGMEPDKNQRSLISVLLEGVALSSIWRESGIPGLFVAPSNYEMGVAERLLPTRP
                     GYEVTLRKVLTTQAEDYQFIVLDCPPFLGALTLNALTAANLLMIPTQAEYFSIYAIRN
                     MMGLIRRVRSQHNPDLTYRLLLTMFDRRNRIHRTLAEQLQSTFGNGLLETIVETDTKL
                     RESPIAGMPIIYQFPKSRAAMQYRALAQEIIQYAQETTAQPNK"
     gene            17792..>22140
                     /locus_tag="PKL11_02200"
     CDS             17792..>22140
                     /locus_tag="PKL11_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12439.1"
                     /translation="MPKKQQHNRINNLFTNLGESLTPQDQSVPGGLPGWTWECDLDGK
                     YVKCGPEVMACLGIRPASFIGQPLSTFSIPTEYSAPLRQILLADTFPATVAIYYQSAE
                     GAFIPVRMQVQRRYSETGEINGYTGISEVLSEMPARVDDSTAMFAPGSEPSTTIFPAT
                     SATTEIRGVAISDGQVLPAIDFWTEAGRESQNQGGLVAHSPAPNRPASIAVPFTLRDQ
                     AGGIVELVDDTRRRAWTQDEQLLVQDVANQLAQALENARLYATVQQELRQRVQAQQEI
                     TRRNRDLATINQIGQQLSRLTGAEEIFNLAHDMIGEVLDNKNLVIAAYDAKQNLISFP
                     VAAQGGQRIQIPARPLGQSVVDHIVRAREPLLIGRGVRRILQAKEIELPEPLPASILG
                     VPMTAGDRSLGAIVLRDFESEDAYTQVEAEILSTVASQIANALENANLFQDVRNALQT
                     IENRERYQSGVAKAVATLAQFGSRSIVEVLEQLGTADECELAFFAQPEAGDLPNQWEL
                     IADWYTSLSSPSLKATSIPIQTRWVNELRENGWLVLQAVDCPVDEQNYISSEGFKSLL
                     LLSVPGKKFTPGFIAFGERNKERTWLNEEINALRAGADGLANTFVREDLVQQIQDTLN
                     ETESLYNISHRLALANTFDEMIEAITLGFRIPTIKRGKLAIFARDAADNIIGVDVVGS
                     WPSLDLNLDQEIGYTDPSYRKFFAIQTPLFLDSIEQAEVPISIQADLRSRSVQTLAVL
                     PLWTSKRQLGVLVLEGDEAYNFTNREIRSLPPLVDQMATSVENRQLFEQTEEVLAETE
                     LLYKISSGIAQAVNLDDLAKMVSQTVMPRHAERISLMTVNSTGDGELLDIEIAGFYDK
                     QGEFQRLGVKVPVANLPITRLMTNDPLVIANATEDSMDSVSRRTLQQLNIGSGCLVPL
                     RSSGHLIGILSASSRKPAEYSSEEVRIMQVTGNGVAVALERQRLLREAQRRALELQTA
                     AEIARDTTSTLSLDTLLESVVNLLLDRFDFYFASVFLLDETGNYAVIREATGDAGREL
                     KDSDYHLAVGSRSVVGSVTSSGTPLVINDVSKSPLYYPHPLLPETRSEMALPLRGAGK
                     VIGALDLQSNRVNAFSQDEIAVFQILADQLAIAIENARAYQLSQEAFENIREMDRLKS
                     QFLANMSHELRTPLNSIIGFSRVILKGIDGPINDTQRQDLSAIYNSGQHLLALINDVL
                     DLSKIEAGKMELAFSEVNVGDVIVSAMSTAVGLTKDKPIQLRHVIPPNLPPVQADSTR
                     IRQVLINFISNAAKFTEEGSITVEAMLSNGPDGSPEIMVTVTDTGPGIAEKDQIKLFQ
                     AFSQVDDSPTRKTGGTGLGLSISRSLIEMHKGRIGLLKSVVGEGSTFFFTLPLPKPEP
                     EPEPTDDSSNVILAIDDDPQVISLYERFLQPQGYQVIALTDPTKAVERAREVKPFAIT
                     LDIMMPERDGWQV"
     assembly_gap    22141..22240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22241..22857
                     /locus_tag="PKL11_02205"
     CDS             <22241..22857
                     /locus_tag="PKL11_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12440.1"
                     /translation="FAITLDIMMPERDGWQVIHDLKSNPETHSIPVIICSIVEDEEKG
                     FSLGAADYLVKPFLQEDLTNAIIRLNADGDIHRVMVIDDDPDDLRLVQKMIEDGGDFQ
                     AILAESGERGLELLETMRPDAIILDLFMPGLNGFAVLEKLRSDAKLSSIPVIILTGAD
                     LTAEQHKQLSEFGKRTLTKGMLRERELITSLEESLRKLKNQRRE"
     gene            22867..24552
                     /locus_tag="PKL11_02210"
     CDS             22867..24552
                     /locus_tag="PKL11_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ12441.1"
                     /translation="MSSQFIVECIDCHNRGPFFVNSTACPNCGSAWREARYDYETIAK
                     TLPLVLHERPFDLWRYRELLPVRSPNPDLSLGEGGTPLIRATNLGMMLGCPNLFIKDE
                     RQSPTHSFKDRQAAVTVAALKEAGVTEVVIASTGNVAISYSAYAARAGIKCWAFLTSL
                     VPGAKMREVAIYGTQVVKITGSYDQAKQVANTFAKQRGLYQDTGARSIPNIEAMKTIA
                     YEIVEQLTTLFGPPTGSGDQGMPIWTAPDWYIQAVSGGMGPLGVIKGFNELFQMGLID
                     RVPKIAAIQADGCAPMVHAWRLGQETATPVQSPNTQIITLATGDPGRTYTLLRQRMLA
                     ASGGTFESVSDEDAYRAMHLIAKMEGLSVEPAAGVAFAGLIKLIRNGQIKPSDRIVVN
                     CTGHTMTIEKNILGEGWSRNVVLSTQAMEDSPEDGLLAALSRVAIDRFPRIAIVDDNP
                     DVRRLIRRILQSQGNYELFEATNGREAIELAKREQPNLIILDLMMPEVDGFGVLQALE
                     ADPDTAEIPVIVVTAKELTPAEKARLKGHIQTLMQKGDFMSDDLQDEVRALLG"
     gene            24569..25513
                     /locus_tag="PKL11_02215"
     CDS             24569..25513
                     /locus_tag="PKL11_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ12442.1"
                     /translation="MNSKQRKTGISAALASALFLGAVPIFGKQAILFGFAPLAVVALR
                     TSIAAALLTILMLIFRRQFFYIFPVGLIGCTVAGVVNGLGSILYYTSLQTLDAGVGQL
                     LYAFYPIFVAFWLMLDRQPISRLTIFRMVLSVPGIYLLIRAGRDGVNLLGAGMMLGSA
                     ILYALHLIINQRVLYEVPAPTVTLYTLYAMAATVVIAYLLFNRTLPPPSTPWWPIVGM
                     GILTFAARLTLFTGVKHVGGLQTAMLGFLELVITVSLAHIWLHETLTPSQWIGTGLLA
                     ASLILVGFDKFTPEKRRTTGLLAWLNPPGIPTFKLPWQ"
     gene            complement(25543..27792)
                     /locus_tag="PKL11_02220"
     CDS             complement(25543..27792)
                     /locus_tag="PKL11_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ12443.1"
                     /translation="MDNLDFIKQLPIFCQLDPLAAAAIANEMQEYPLPAGQTLFRQAD
                     PGDSLFVVKSGRLRIYLPQPDSPDVPMGVLNPGDCTGEMSLLTGQPHEFTVIAEGDAL
                     LLHLSRQAFDKLGAVHPGMVNELACALLPRVRQVQAQLALTRVFGPLDEAAMSELLSH
                     LEWRRLDSGQVLCRQGEPGNEMYIIVQGRLRFASEDQGQMHDLGEVGAGEAVGEFALL
                     TEKGSLESRRSATIYATRLTDIIVISRQVFEDLLCQFSQVLLKLSRRIIQRELRVSKT
                     VAAPVSAQVIAVVPLQPDGLGSKFASQLAETMGMLGTTLFLDSRRFDSLYGKPGASQT
                     PLDHPTSLLLDTWLDERERRHQYALYDVGPALDADSHLTPWAQRCVEDADLILLVTDD
                     DARPEISAVEMVLPSASTRARVELVLMHRPDCKVPSGTTDWLEKRHLGNLPVRDHHHI
                     RHDNPADLRRLCRRMSGNPIALTLSGGGARGWAHIGVLQALAEADIEVDWVGGASMGA
                     IIAGGYALDWSPDVLRQLAEKFSDPKKLLDYTFPYTSITATRRITTLLQTLCGEAMIE
                     DAWRPYFCVSANLTRGEEQSHTRGPIWRAMRSSMAFPGVFAPILDQNGCVLIDGGAAN
                     NMPVDRMREFCPTGTVIGIDLLTSSPVKGQYNFGPSLSGWQALFSKLNPFGGKIKSPN
                     LLNIVDGIVYSNNRYRLNETGHCADLIIRVPVEKFGLLEFDQYLQIIEVGYTAAKEQL
                     KNFRFDVPA"
     gene            27766..28920
                     /locus_tag="PKL11_02225"
     CDS             27766..28920
                     /locus_tag="PKL11_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNZ12444.1"
                     /translation="MFDEIKIVHFSLDHFEIYGSWGRIIIYVISVLISYDLSTEFTPG
                     NNRSKIGQRVQPLLSKVQSMKITGRPQNQWGWVRLISAIILAGVFFWTLLAVILKAFP
                     ISWQVVQDYSIINGVELAIIPLLLTLFAGWMEEQDVKAANEKASHQETEHLLTMRRKA
                     VLKHFFETTNAILAEDHQLEHDREKLQDTVQLTLPELDGKGKGEILIFLHEKGLLSGE
                     QSIDLHNADFREVELHRAHLNGITLERIDLSRARLEGAHLAGGKLHGANLAKAWLRHS
                     NLQRAMLTGCNLRSAHLEGANLEGADLSHACLVDAFFKKANLRHCTGLSISDQHPDEG
                     AARHNQSVGAADLEQAILIDAILPDGQKFTNEKGRAFLRRKEIEELIDRL"
     gene            complement(28928..29410)
                     /locus_tag="PKL11_02230"
     CDS             complement(28928..29410)
                     /locus_tag="PKL11_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNZ12445.1"
                     /translation="MGRLILITGNRGTGKTRFCTLVARRMQQRGFDVAGMLSPARFEG
                     TLKIGIDALDLRSNQSRPLAQLIETNPHSATQTGHWAFDDLTLAWGDSCLRAATPCDL
                     LIVDELGPLEFIHAKGWLSGLVALDSFRYRLALVVIRPELMEAAQTRWPGCGIFHIGG
                     "
     gene            complement(29398..30174)
                     /locus_tag="PKL11_02235"
     CDS             complement(29398..30174)
                     /locus_tag="PKL11_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12446.1"
                     /translation="MNPVLAFDAVTYSYPQADHPALDDLSLTLIPGETTAILGPNGAG
                     KTTLLRLAYGRLHPASGQIVLQGAPLAQYTHRQIGQRVGLVPQREYNPFEYSLLEYAL
                     LGRAPHLHPLELPGATDCQIAQTALERVGLADRAHQSIHTLSGGEHQLLLIARALAQQ
                     PALLLLDEPTAHLDLSNKQLLVRQLRSLAAEGVTILLTTHDPDVAAALAAQLILMRSG
                     RVLRAGPLAEVWNDDDLSTAYGISLRTRQLDGKRLLQWDD"
     gene            complement(30174..31184)
                     /locus_tag="PKL11_02240"
     CDS             complement(30174..31184)
                     /locus_tag="PKL11_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ12447.1"
                     /translation="MHPLARTPRAARRALALLGVLLLSLLALSLVLGRYPNLGIMPPA
                     RLLDDPLAQQLVWNLRLPRLIAAVLIGMSLAAAGVVFQMVFSNPLVEPGFLGVSQAAA
                     FGAALAIIALGSAAWLVQGGAVFFGLLGLGASYLLARRVRYGGWVLRLVLSGIAVSAL
                     FSAGVGMIKYLADPLRQLPEITFWLLGGLYGVTWPRLLAALPLMLGGLVVMLFLRWRL
                     NLLTLDEDAAAALGASGSSERLAVLLAAVAATAASISIGGLIGWAGLITPHLARRLFG
                     ADARYALPGALLIGAIYLVVCDNLARLLLVGEIPLGILTSLLGAVAFLTLLTRPLLQP
                     QS"
     gene            complement(31190..32263)
                     /locus_tag="PKL11_02245"
     CDS             complement(31190..32263)
                     /locus_tag="PKL11_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12448.1"
                     /translation="MKKYITLLMLVFVLALSACTPTPAPANASITIVDAQGRTITLPA
                     APQRIVIAGRASALLADAVYLFPEAEDRVVAFSTTSQGTGDFISVLDPNFADKAAFGT
                     DVGPEQVVAFTPDLVIMKSYMAEKLGTPLEALGIPVIYLDLETPEQYTRDLNILGQVF
                     QNEARAAELTTYYADVVQRVNTGLAQSQPRPRVLLIYYTNRDGEVAFNVPPVGWLQTT
                     LVQLAGGEPIWQDIELGSGWTKITLEQIAAWDADQIYLVAYTVNAADVVSQLEQDPAW
                     QELRAVKAGQLHPFPVDYLSWDQPDPRWALGLLWLADTIQPGVLPVDMLAETQTFYQK
                     LYGLDQAAFEQLIAPVLPKNLEP"
     gene            32558..33034
                     /locus_tag="PKL11_02250"
     CDS             32558..33034
                     /locus_tag="PKL11_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ12449.1"
                     /translation="MAEIRDIWLHAHHMIRSARQIINENLRSLNLSSAEGNILLHLLT
                     QEQEMGQEQLVEQLDISKPAVSRALNSLVAKGYATWLPDPQDRRAHRVRLTEQAQKIG
                     PDIERAYNQIYALAMRGISQKELDDFVKLFGRISENFRCAQAKKITEENYAAQRLG"
     gene            33012..33737
                     /locus_tag="PKL11_02255"
     CDS             33012..33737
                     /locus_tag="PKL11_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12450.1"
                     /translation="MLHNDWVSIGLYLGFIVVFGLPAILLKVGFNVPFEILRKMYHMV
                     IALSIFPLVMFFKNWYMAVLSAFLLVVIAYPLLALVENTAIYKRIAVERSSGEFKKSL
                     IVVQVSIALLIFIFWGLLGSEWKYIAVVAVMAWSFGDAAAALVGKRFGRRQIRHPRIE
                     GAKTMEGTQAMYFTAGLAIFLTFMFYAGQPWQVSLAVALLVAPISAVVELFSNRGMDT
                     LTVPISTGLAVLSLMSLFSLLGT"
     gene            33825..34391
                     /locus_tag="PKL11_02260"
     CDS             33825..34391
                     /locus_tag="PKL11_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNZ12451.1"
                     /translation="MTGIALISSHSTTQLADFLRRGVELVAIFISWWVFRRLHQNGAV
                     DGVQQARLERTAGLSTAGAMAFSGVILLGVTVSRLSAFEPGGNVISGLVIAVLGLVTN
                     GWFWRRYTILTREQYSAVIAAQQQLYRAKTTVDLCVTAALMAVAIAPNHPMTRYIDLL
                     GSIMVAVYLLWSGLRAAQKHPIIRQAKL"
     gene            34547..35383
                     /locus_tag="PKL11_02265"
     CDS             34547..35383
                     /locus_tag="PKL11_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ12452.1"
                     /translation="MKSPLNLSDIQIRDIFIDGQDGAQIRLRVYQPGTPPVKAPALLW
                     MHGGGYVMGMPEQDDIYVLPFVREVGLVVVSVDYRLAPDYPFPTPLEDCYAALQWMTA
                     QAEIPGIDPNRIAVGGSSAGAGLAAALAQVAHDRGEIHPVLQLLVYPMLDDRTGGLKD
                     IEPKAHVVWNNTSNQFGWESYLGQSCGAETAPAGSVPARRDNLAGLPPAWIGVGTNDL
                     FYDEAAAYARRLSEAGVPCELVPVPGAFHAFDLFAPKMQAVRDFRQAQVVALKKALYG
                     NG"
     gene            35640..>35904
                     /locus_tag="PKL11_02270"
     CDS             35640..>35904
                     /locus_tag="PKL11_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ12453.1"
                     /translation="MLSIEQIRHYPFFKGLSEDELQMLAGCMTKRCFNKNAYIYHQGN
                     PAINLYLIESGMVRLFFCNDTGNEFIMNLVGPGSVIGHSLAMNN"
BASE COUNT         8704 a         8699 c        10012 g         8389 t
ORIGIN      
        1 tggggcgaaa cgccgcgaac atatccggta gacattgaga taaaggccta tgaccgacag
       61 ggtctaatgg cagaaatatc caccatttta gacaatgaag gtgtcaatgt ccgcgatatt
      121 cagatgcata ttgcacataa cttggctacg atccgaatta tggttgatgt tcgggatatc
      181 gcccacctca gccgggtgct gactcgcctg gagaatattc ctaatgtgat ggaggcgcac
      241 cgaacccgac ctggataaat ttctccaatc ccctcgaagg cttgtcggat tgctatcgtt
      301 ggggtaaaat cgcacactac atgaaactgg acataaaacc acgtcgtact tgattaagca
      361 cgaagatact catgattatg gaggattaat atgccaaaag ttcgctgcca ttatctcgat
      421 tgcggttttc tggatgaagg tgtctgcact gcagcgatgg tcgagtttga tcccgattct
      481 ggctgtatga cttattctgc caatgccgga gcgttgaatg gcgacgattg ggaagaagaa
      541 gacgaactgg aagaatggga agaactggaa gacgaagaag aagacgatga gctgtggctt
      601 gatgaagatg aagaaacgga agcttgagag acacccctat cgagcagaag cttcgtcata
      661 attacctcgt caatctcgca gatgggggct ttttcggctt tgcaatcggc gcctcatcat
      721 tttcagccat cctgccgcta tttgtagccc ggtttacaga ctcggcagta ctgattggac
      781 tgataacagc aattcataat gtagggtggc agctgccgca gctttttctg gcgcggtgga
      841 ttgccaccct gacgcgtttt aagccctggg tgttaaagct gaccatccac gagcgggtac
      901 cctttttggg gctggcgctg attgccggac tcattttcac ccaaaaaccg gtattatctt
      961 tgatactgac ctttgttatg cttgcctggc agggtctggg cagcgggttt gccgctaatg
     1021 tctggcagaa tatgattggg aaaatcatcc ctggttcaat tcgagctacg ttctttgggc
     1081 ttcaatcatc ggcatccaat ctgggtggaa gtattggcgc tgtgatttcg gggatcctgt
     1141 tggaccggct gccctcaccg ctggattttg tgttgtgttt tttaatcaca gtcggtttga
     1201 tgggaatatc ctggtttttc ctggcattaa cgcgtgaaga ggtcacccca ccacccactg
     1261 ccgaagatgt tcctcaacca acagccgaat tatcacggca tattttacgg tccgatgctt
     1321 catttaaatg gtttttggta gcacgcaatc tgtttcagtt tggcacgatg gcgttttcgt
     1381 tctatatcat ctatgcggtt ttgaaactgg ggatgagtga ggtagaagcc gggcttatgg
     1441 cggcagcgct caatattacg ctggtgattg caaacccggt gttgggatgg ctggcggacc
     1501 actggaatat tcaggctgta atgacgctgg gcgcagtggc ttcgctgctc agcgcgctgg
     1561 tggcttttct ggcgccttct ctggggtggt tttacctggt gatcgtcttg caggggtttg
     1621 ccaacgtcac tttctggacc attggaatag ccttcacgct tcaatttggg actgaccgcc
     1681 aacgcccggt ttatattggg ttagccaaca cgctaattgc gccaagcaca attctggcgc
     1741 cactggtggg gggctggctg gccgacgcga ttaactacca ggctaccttc ctggtttcag
     1801 cgggactgag cggcctgacc gctctaatct ttcttctgtt tgtcaaaaat ccgcctgtgc
     1861 gcaaggcaga ggcaggttaa acccgttcga tcacaacccg ctccgggcta atttcgatgc
     1921 tccacatgcc gtgattggca aagcgataaa cagccgtcag gtgatctggc tcgcggacaa
     1981 catccaccag ttcaatcggc gttgagaatg caaaggtgat gagatccggg tcccactcgc
     2041 cttgttgttc ggggtcgtag ccttgaggtg gttcataggc gcgctcaata tttgcatcgt
     2101 tcaaaagcaa gcgcagcagg gcctcgggaa catctatgtt tgccaacaag ctggctgagg
     2161 cctgggcttc catctcctga acgcgttcct cgggggtcaa tcgccattga acgggagacc
     2221 agacgccggg cagttcatct acggggaggg tttgggggtc aatgacacga gccatgagca
     2281 atctccttta ggtgtaatgt gatattgaga gtatagcctt aagtggcggt ttatgctaat
     2341 attcaggcca tgtcaatttt gccggattcc agggattgtt ttttggttgg aaagattgcc
     2401 gtaaaggggg agttgattct tgcgccgatg gacgggtatt cagatttgcc gttcaggtca
     2461 ctggcgcgcc gcctgggctc ggcaatgagt tataccgaat tcatcagcgc aattgatgtg
     2521 attcatggtc gcccggggct ggaacgccgc ctggcttacc tggaagaaga gcgccccgtg
     2581 gttgtgcaat tgctggacaa tgacccggaa cgactgctgg cagcagcgca aaagctgcgg
     2641 tcgctaaacc cagatgtgat tgatattaac ctgggatgtt cagcaaagcc tgtcgccggg
     2701 agaggcgcag gtgcgggact gctgaaggag ccactaaaaa ttgctcaaat atttgcaatg
     2761 ttaaaccaga cactggatat tcccgtgagc ggtaagatcc ggctgggctg ggatgatcaa
     2821 agccgcaatt atttgcaggt ggctcaaatt attgaagaga acggtgggag cctgattgcg
     2881 gtgcatggac gcaccaaaac ccaggcatat accggaaatg cagattggga cgcgattgcg
     2941 gaaatcaaac agcgcgtgtc tattccagtt atcgccaatg gggatgtgca gacggtggcg
     3001 gatattgacc gaataaaagc gcatactggc tgtgacggag tgatgattgg acgggcagcg
     3061 gtagcaaacc cgtggatttt tgcacgaaaa gaccgggagg aaatcactat tccgcaagtg
     3121 cgaggaatca ttgaagaaca cttggacgcc agcctgaggt tttatggcgc agaagccgga
     3181 ttggttttct tccgcaaata cgcttcccgc tatatggaaa gctatgggat ttcacgcgag
     3241 cagagacagc gcttgatgac ccggccgaca tcggttgaat ttctggatac actagataat
     3301 attttcaaag ctatgctaga atgataagcg atactattcc tcagttgtta ttcgcctcag
     3361 ggagtcgcaa tgactaaatt tatcgcagcc atagaccagg gcacaaccag cacccgctgt
     3421 attgtttttg atcactcggg caacgtggtg gcaatggatc aacaggagca tactcagatt
     3481 tatcccaaac ccgggtgggt agagcacgat ccactggaaa tctggttccg gacaggcgag
     3541 gtgctgcaaa acgcactgcg gaaatcggcg ctgacttcag aagatattgc ggccattggg
     3601 gttaccaatc aacgcgagac aactgtggtg tgggaccgga caacagggat gccgtactac
     3661 aacgcaattg tatggcaaga cacacgcacc gacaccatct gtaatcgact ggctgctgat
     3721 ggcgggcagg atcggtttag aggcaaagtt ggactgccgc tggcaactta tttttcgggt
     3781 ccaaaaatca aatggctatt ggataatgtg gccgggctgc gcgcggcggc cgaaagcggg
     3841 cgcgctattt ttggtaatat ggatacctgg ttgatctgga atttgaccgg cggcacgcgc
     3901 ggcggggtac atgtgaccga tgttaccaat gccagccgca cgatgctgat gaatttgcat
     3961 acgttggatt gggatgatga gatgctggct gtgctcgaaa tccctcggaa aatgctgcca
     4021 gaaatccggc cctcgtcggc aatctatgat tacgcgtcgg gcgttctgcc cagtgtgccc
     4081 gtctcgggcg atctggggga tcagcaggcg gcgctgtttg gacagacctg tttcagcccg
     4141 ggtgaggcca aaaacactta cggcactggc tgttttatgc tgcttaacac gggtcagcag
     4201 cccatctaca gtcagaatgg cctgctgacc accctgggat acaaaatcgg caatcatccg
     4261 gcaatctact gcctggaagg ttccattgcc attgcaggtg cgctggtcca gtggctgcgt
     4321 gacaacctga aaattattga gaaatctggc gacattgaac cgttggcgag gacggtgaca
     4381 gacagcggcg gcatttatat tgtgccggct ttttcggggt tatacgcccc ctactggcgg
     4441 tcagatgcgc gcggggtgat tgttggcttg acgcgattta ttaaccgggg acatattgcg
     4501 cgcgctgcgc tggaagccac cgcttaccag acgcgcgagg tattggacgc catggagcag
     4561 gattcgggcg ttaagctctc tgcactcaag gttgatggcg gtatggtggt aaacgatctg
     4621 ttgatgcaat ttcaggcgga tattctcaat gtgccagtta tccgaccgag agtggcggag
     4681 acgacagcac ttggcgcagc ttacgctgcc gggctggcgg ttggatactg gaaaacggtt
     4741 gatgacctgc gtgtcaactg gggtattgat cgggaatgga agccaggaat cgaagaagcg
     4801 cagcgaagcg ttcagtatgc ccggtggaaa aaggcggttg cccgcacact ggattgggtc
     4861 gaataagtta aagcgcttga caagcccgat tagactgacg tataatgcca ctcacaccag
     4921 cgggtgttgc caagtggtaa ggcagtagct tcccaagtta ccattcgcga gttcgaatct
     4981 cgtcacccgc tcagaaccgc ccgccagaca aaaagcaggc ggttttcatt ttctccctgt
     5041 caattttgtt aagtggtttg ggggctttta tgctaaaatt tatgcggagg tctcaggtca
     5101 gatgtcacac aaaatcctat acgttgaaga taatcccgat aatcgcatgc tggtacgccg
     5161 acttttaatg tcttgcggtt acgagatcac tgaagcctcc tcggcacgtg aggcgctgga
     5221 agctctaaag acccttgttc cagatttgat cttgatggat atcaatatgc ccgacatgga
     5281 tggttatacg ctaaccgcca tcatcaagga aaataaagcg ctggaaaaag tgccggttat
     5341 tgcattaacg gccaatgtga tgcgcggcga tcgcgaacgc acgctcagtg cgggctgcga
     5401 tggctacatt gaaaaaccca tcgatattga tcgctttttc gaccagattg aagcttttct
     5461 ggcgatgtat cagaggtaac agctcatgcc caacgatgat tcttcttctg tgcaaggaat
     5521 tcccaagggg acaactgtcc ttgtggttga ggataatgtc agcaattttg tgctgattgc
     5581 gcgtctcctg ggctttttag atattcactg cgaatggaaa acttcggggt acgaagtggt
     5641 agagtttgcc gatacactgc cacgcattga tttgattctg atggatatcc ggctgcctta
     5701 cgaggatggc tacggtgcga ctaagaaaat tcgccaatcg cccaagctca aaaatgtacc
     5761 tattgttgct gtgaccgcag aggccagcgt ggagcaaatg aacaaagctc gcaactctgg
     5821 atttgatggg tttctttcca aaccattgga tccagatcgt tttcccgaac aaatcagccg
     5881 catcctggcc ggtgaagccg tctgggaatg ggaataagga gcgctcccga tgcctgcaaa
     5941 ggtttcgaaa acaccgaagg atgccccccg ccaatttaaa agtagtttag cccgaaatac
     6001 tatcatcatc attttgatcc ttgcattgct gcctgttgta gccattgggg gagccacact
     6061 tttacaaatc caacaactgc taaaaaccca gaccggcggc caatttcaga ccgttgcgca
     6121 aaactttgtt gctcagcttg aatcaatgac tgcagcacgg cagtctttgt tgggggacat
     6181 aaaatcggat gatgtttttg ttgagaatct gaatattttg atggatccgg cctcagatga
     6241 atatgcgcgt tttgaggcca aaactgctgt ttcaactacg gtttcaaaga ttgcattcag
     6301 cccaacggtg gaaaggttga tcgatggatt agcaatattc caaccggatg gcaatttgtt
     6361 gtttgcaaca agagaaagct gggagagtaa taccgaggcg ctggatcccg cagtccggcg
     6421 gcttttaggg acaaatgatt cggcgctggt ttacgatgtt ccgccttttt atcccaacca
     6481 gtttgtcctc atcagcgcca aagtttttgt gagcaacgac attgatcaga ttgtcacaat
     6541 gatgtcattc agtctaccga aagaccccca ggaactttta aatacagcca agatttattt
     6601 ccccaatgcc attgggtatt attacacagc agataaaacc ttagtttaca ttgatgaaac
     6661 cggaaaactg gctgaaattg gcttaaccga aagccaaatt gatgatatgc aaacactgca
     6721 aagccttagc agccagaaga agattcagga gacaactgga ctggacgccc aaccggctta
     6781 tgcctatgtg caacccgttg gcggcctgaa gacatctctg gtgcttgaga tgccgcgtaa
     6841 aattttggat gctcagatcc aatcgcttgc cagggtgaac ctgttattaa ttggcatcag
     6901 tgtcctcatt atcggggcgc tggtatacct ggggacgaca agcatcgtgc gcccgctggt
     6961 gcggctggca aacaacgcca agagttttgc gggcggagac tggtcagagc gcgcccgcgt
     7021 caatcggaat gatgagatcg ggctgctggc taactctttc aatgagatgg ttgagcaact
     7081 gagcgtcctt taccgctcaa tggaattgaa agttgaagaa cgaacatcac aactgcgcac
     7141 agcatcagaa gtagcgcagg ttgcaacatc tgtaaccagc caggcggaaa tggccgaaaa
     7201 ggctgcaagc ctgattaagg atcggtttgg ctattactat gtggctgtct atttgatgga
     7261 tgcaactgcg agttatgcta ttctacgatc tgtggcctgc gacgatgccg agttccggga
     7321 cctagtagga tatcgcattc cagtaaattc ccagacgatg attgggtggg tggcatctca
     7381 cgggcagtct caagtgtcgc tcgacatatc tccgacagag gcgattgagc agggacgcaa
     7441 tataatgccg ttatcgcgct cggcgatggc tgtgccgatt gtggttggca atcaggtgtt
     7501 ggggattgtg gaagtacaaa gcacaaagcc caatgacttt gaccccgatg ccatttcagt
     7561 gttccagacc atggccgacc agattgcaac aggattcaga aatgtgtcgc tgttggaatc
     7621 ggcccaggtc aatcttgatg aagtggggct gctgtaccgg tcaagccgac aaattgccca
     7681 ggccaaagac gagcgcgaag tgctcgaaaa tctttctgaa gccctgtcac acagtcagta
     7741 cgtcagtatt gtgttggaag cggcggaaga tcatctggtg atccgctcga ttcatgaccc
     7801 caaaggcacc gggtttgact caacgctgcg cggcgtggct ttgcccgtgc ggcaggggct
     7861 ggggcgtttg tcagagaccc ccatcttatt gattgatgac ctgcgcactt catcagaatt
     7921 tgagaacttg ctgaccttcc tggcgcggcg cgaatgccgt acggcagcct tgatccctgt
     7981 tttgatggat ggaaagccta ataaagtaat cgccattggg tctcgagagg gtacggcact
     8041 ttcagcaaat gtactgcagc cttattccag tattgctgaa gttgcgggct ctacgttagg
     8101 gcgcttaaat ttggttcgta gtctcagcgc tcgcctgtct gatattgaaa gcattgcgga
     8161 cttcagccag gccatttcta ctgaaacaga tctgagccga ctgtacaagg tattgcacga
     8221 ccaggccatc aagacatttg gaggagatat tttcttcctg gtggctctgt ttgacagcaa
     8281 acacgacatg attgaggtcc cttattttta tcagggcgat cgattcgttc agatggagcc
     8341 ctatccagtt ggcgatggtc tggtgtcaca tatcatccgg accaaacagc cgctgatgct
     8401 ggtcaagaac atcgaacaga ccatggtgca aatgcgcgcc attcacgcag gcggggtggc
     8461 caagtcgtgg ttgggtgttc cgctgattgt gggtggtgac ctggttgggg ttctgtcggt
     8521 gcaggatatt gtcaacgaag aacgatttga tgaaggcgac ttaaatctgc tgctgacgct
     8581 tgccccccag attgccaacg cgatccgcaa tgcacagttg ctgaaacaaa cccaggataa
     8641 cctgcaggcc tatgaagcag aacgcgcatt attgaacatc ctgcttgcca acacaccgga
     8701 gcaaatttac atcaagaata ccgcgggaga atatcagcgg gtcagccaat cgtttgcaga
     8761 ttgggtaggt attagccgac ctgacgatat tgcgggtaag acggatgctg atttgtttgg
     8821 cacagaattc ggcgaaaaag tttttgaaga agaacgcact ttactctctt ccggaatcaa
     8881 ccggattggt gaaatcgaac aaactaaaga caaagctgga aatcttttct ggggaaccca
     8941 ttctcgaatt ttgatatccg attcggctgg gctaacaact ggtattctag gtattcggca
     9001 ggatattacc gaaattaaga ttgctgaaca actttctatg cggcgggcag accaactgca
     9061 aacgattgct gatattgctc gcgaagccac tggtacgttg gatgtgaatg aactgctgaa
     9121 gaaggcggtt aatctgatcc gtgaccggtt tggcttctat cattcatcca ttttcttgat
     9181 tgaccccaat ggagaatacg ccgaactaag ggaatcgacc ggcgaggctg gcgcaaagat
     9241 gatggcggct ggacaccgct tggttgtggg gtcggcctcg atggttgggc aatcaacccg
     9301 ccgcggtgaa ccggtggttt ctaatgatgt taccgccgag cccaactact accccaaccc
     9361 catgctgcca gaaacccgat ctgagcttgc tattccatta cggtctggcg gacgaatttt
     9421 gggcgcgctg gatgttcaaa gtacccaaac gcaggctttc tcaccagaag acattgatac
     9481 cttacagctt ctggctgatc agattgcgat tgcggtcacc aacagtaatc tctttgcccg
     9541 atctcaagac gctctgattc gacaacgttt ggtgaacgaa attactgtga cagccgccgc
     9601 agcacaaagt ctggacgaag cgattgcgac agcggtcaaa ggcctgcgcg aagcgatggg
     9661 cggcgaccgg atcagcgtct tcctggtaga tgaagggcag cggcagatgc atttacacac
     9721 atctgccggg tatggcgaag gcgagatgga aacattgatt gttccatttg gggaaggcat
     9781 tgttggagaa gctgcggaaa ctgcagaaac gatccgggtg gacgacgcgc aaaacgatcc
     9841 acggtttatc gcgatggaag aagatatacg cagtgagttg gctattccaa ttcactttac
     9901 cgaccaattg atcggcgtgc ttaacctgga gagtaagcga ctggcagctt acgacgaaag
     9961 cgacgagcaa attctggaag cgttaggcaa caaccttggg gcagtgattt caaatgccca
    10021 gttactgcgg caaatccgca tgcaggtgga acgccagcag caactatatg aaattaccag
    10081 taagattcgc cgatcttcag atttgcaaac catcctgcga acatctgcca ctgaaattgc
    10141 ccgggcactg ggagctgagc gggcagaagt tcgactatca acccgaacga ttggcagggc
    10201 agctgatgag ccaaccgcgg ataacggcca tgagcccgag attgagaaat agagtgttgc
    10261 tatgacaaca accacaagaa ccattcgatc gcgaactcgc ctgcaaaacc gtatcctgtc
    10321 ccttattctt gggattgtgg tgcctattct cattggcgtg gtcatggcat ctctgcggtt
    10381 tacagagagc accgtgacgg ctcaaaccga agaacagctc gtttatgtca gtcagggtgt
    10441 tagcaacctt ctggacaatt ggctttccct aaacaataat caattaaaga atctggcgct
    10501 gcagcctgga atcatcagca tggatccggc gcagcagaag cctattctgg aagccatggc
    10561 cgcggccaat ccatatatgt atctggtgag cacgaccaac aaaattggga tgaatgttgc
    10621 ccgcagcgac aataatgagt tgatcaatta ccttgaccgg gcgtggttca aaggcgccat
    10681 caacgattat gaatttacct accaaatttt gatcggtcgg acaggcggag cgccagccct
    10741 ggtaatgtct acgccgatta agcaggacgg cgccattatt ggtgtggcga tgtttgcggc
    10801 tgaattgaac cagattacgg acacggtttc cagtatcaaa gtaggcgagt cgggttttgc
    10861 gtttgtggtg gacccctata accgtgttat ggcgcatccg aaccccgaat atgtggcaaa
    10921 tttgctggat ttgagtgagt accctcctgt tgtggcttta cggggagggg aaagcgggat
    10981 ttataacttc accgatgctg aggggcgcaa ctgggtttcg catttaacaa aaacagctga
    11041 tgactggggg attattgttc agctacccca atctgaatat ctggcattgg ttaatcggca
    11101 gcgtctgaca gcaattatca ttctggcggc tggcgttgtg cttgtcttga gcttgaccag
    11161 tactatttta cgtcaggtaa ttcgcccggt gaaggttttg acgcgaacgg ctgtggcgat
    11221 ttcacgtggt gacttgaacc ggactgcccc ggttgaaagc gacgatgaaa ttggtacgct
    11281 tgccatgacg tttaataaaa tgactgcaca gcttcgacaa actgtggatg atctacaaat
    11341 tcgggtggat gagcgcacgg cagagctggg taatgcgctc tcacagttgc gcacggcggc
    11401 ggatgtgtca cggtccatca cacgcgtgat ggatgtgcaa acgctgctgc aagaagttgt
    11461 ggatctgatt aaggatcggt tcaatcttta ttatgtaggc gtcttcctgc tggatgaaac
    11521 tggcgagcag gctattctgc gtgcaggcac gggcgaggcg ggcaccaaaa tggttgccgc
    11581 tgggcaccgg ctcttacggg gtggcgcgtc catgattggt tgggctatct caaagggcga
    11641 ggcgcgcatt tctcaagagg ttggcaccga aaccgtgcgg ttcaagaacc cgctgcttcc
    11701 cctcacccgg tctgaggttg ccattccgat cctcagccgt gacaaagcac tgggcgcttt
    11761 aacgatccaa tcaaccgagg cgaacgcatt tgaccaggat gatattgtgg tgctgcaagg
    11821 tgtggcagac agcctggcgg cggcactgga taacgcggct ttgtttaacc aaacccagga
    11881 gtctctggac gagattagag cgcttaaccg ggcctataca gaagaggcat gggggcgaac
    11941 gctggcgacc tacggcacaa tttctgccga gcatgtcaat ccggcggggg tgcacggcga
    12001 tcagccagcc catcaggtgc gagtaccttt gatgctgcgc gaaaatccca ttggagaagt
    12061 tgttctggat atgagcggac gcgacctaac agctgatgaa ctggaatttg tggaggcgat
    12121 cacatctcag gcggctctgg cattggaaag cgccagactg cttgaagaat ctcaggctta
    12181 tgctttgcaa gagcggaaga tgagtgaaat gaccagccgt ttctcgcgcg ccagcaacat
    12241 tgatgaaatt ctaaaagctg cggtaacaga gcttgggaac ctgcctaccg ttgcagaggt
    12301 tgccattcac ctgacaccgc ctgaacaggc tgagggagcg taagggagac catcatgaac
    12361 aatgccggca ccccccaaca aaccaggcca cagggcacta ctgaacacag cgatattttg
    12421 cggcttttgc aggagcgcat tctgcgtaat attctccttg gtgccgtaat atttggtacg
    12481 cttatcttta tctatgcaag ctatgtggct gtatcgcggc agttatgggg attgctgatc
    12541 ttctatgcag taacctatgc aattctgctt cttgccgccc tggtgcgcag gctgccttac
    12601 gctatccgca gtactgctat tatgattgtg gtttatcttc aaggcacggt cagtatgttg
    12661 ggaagtacgc accctgggtc agggatgatc tatttgctaa ccctgacgat tctgggtgcc
    12721 atcttattca acttaagggt tggtctgtta atgatggggg caagtgtttt aacactgatt
    12781 ggcatcgcca ttggggttaa ttcaggggtt gtgccaattt ctgagttgtc acttctggtt
    12841 gggacatcta atcagacaat tccctggatc acttatattt tgctgtttgt actgctgggc
    12901 acggtagcgg tttcttcggt tggcagcacc acaaacggct tgaatgtcag tctgaccaat
    12961 cagcgacact tgacacgggc gctggcacaa gagcggaata ccctggaaaa ccgcattcag
    13021 gaccgcacaa ctgaacttga gcagcgagca gcccaattga ccgcagccag tgaagttgcc
    13081 cgccagatgt ctgctgaaac ggacctggac cagctactca agaacgcggt gcaactcatc
    13141 aaggacaaat ttgggctgta ctatactgca gtgttcctgt cagatgaaaa ggaagagttt
    13201 gctgttttgc ggtctgggac aggtgaggcc gggcaggcga tgcttgaagc cgggcacagg
    13261 ttgaaaatcg gcgaggaagg gattgtgggg aatgtagttt accggggtga gccgcgaatt
    13321 gctctgaacg ttggcgccga tgcaacacat tttaagaacc catacctgcc gcagacccag
    13381 tctgaagctg ctttgcccat gcgggttggc agccgtgtga ttggcgcgct ggatgtgcag
    13441 agcgaacgcg agcaggcttt tggcgatgag gatatccgag tgctgcaaac tattgccgac
    13501 cagctggcgg ttgccattga gaagacatcg ctggttgagc agctagaaga aagcctgttg
    13561 aagcttgaaa cccgctcacg cgagacgacg ctcaaaacgt ggcgctctca cattcgaagt
    13621 tcaaagcgcc catttgctta ccgatatcac cgggcaactg ctgaaattga gccggctccg
    13681 ccgatcagcg aaacagctca ggctgtattg aaagccggga caccgatcat tcggagtatt
    13741 ccggctgaag aaaaccagcc aggaacgacg atggtctcag tgcccattaa gatgcgtgat
    13801 caggtgctgg gcgtcttaga tgttcgtctg gatacgccta cggcgtcgca agaactgatc
    13861 aactatgtgc aaatgagcgt ggaccggctg ggtgtggcgc tggataatgc ccgactgcta
    13921 gaagaaattc agaatcgggc ggagcgcgaa catctggtca gcgaaattac cggtcaattg
    13981 cgtaaatcca gcgaggtcaa caccatcctc agcaccgcgg cggttgaact tgggcgtgcg
    14041 ctgggcctct cagaagttat ggttgaatta cgccaaacgg atgcggacat ctaaccgctt
    14101 acccggagat ttattatgaa tcaatccgaa ccccaaaata caacttcgac aacacaggca
    14161 gggccagccg agaatcaaat gggactcgac cggcggattg ggctttatac aggcatcggc
    14221 gccgggctca ttgggctctt attcctcagc cagggtgtaa cgcatccggt catccagttg
    14281 tatattgtgg ctgcggtttt tatgctggtg gcgctagtca acattttcag cctggaaagc
    14341 ataaccttca aggatccaag gacttccgtt tatcttggtt tatcggcgct gcagcttgct
    14401 ctggttgtct ttgcgcacct gtttactaac acatccctca tctcggcttc tttgattatt
    14461 atcatcggcg tgtttacgat cagcagtcag ttccgcgggc gggatgttga cctgggtttg
    14521 attattaccc ttgtggcggc gatgctgtct ggtctggcgg gaacactgac aaccatcggg
    14581 cagtatgacc tgaacaattt cccgctgttc atgcgcatca tgttgatcgt aattgtggtg
    14641 gcatacatcg ttgtattggg tatcatcgcg atccgaagct tgacgagcac gattacggtt
    14701 aaattgatca ccagttcgct ggcgattaca ctgattccct tgctgctgct atccaccatt
    14761 caaacggatt tcatccgcac gattgtacag aatcaaacca accaggcgct taaactggcg
    14821 ggtgaagaaa ctgcgaatcg aattgacgcg tttttcagtg ataatcttga tcagatcgag
    14881 cgtgcatcta accttgcagc ctttatcaac tacctcaata ttgacccgac ggaacgcaac
    14941 ggcagctctc aggaacagca actggcattg acgattaact cgttgaaagc gcagcaggaa
    15001 gcttatttga cctcgattgg tctgctggat ttacagggca ataacatcta tgactcgaat
    15061 ccatttgagg ttggcaacaa cgaggacacg agcgagtatt tcattcagcc cttgcgaacc
    15121 gggcaaatct ttgtgtcttc ggtgttattc tctgaccaaa acggagatgc gtatttatac
    15181 ttcagcgcac cggtgcgcaa tgcctcgggg caaatgatgg gcgtgctgcg aatgcggtac
    15241 gatgcgctgg tgctgcaaga ccggctgggc gagtctatca acctgttagg ggcgcgatca
    15301 tacccgatgc tggtggacga gctcaatatt cggatagcag atttggcccg ccccagcgag
    15361 gtttataaag ccatcgctcc actgacggca gaccagattg caagtattca gaaacaaaag
    15421 cgcctgccca atttgccggc cgaacaactc tacaccaatc tgcctgaatt tgcagccagc
    15481 ctggaccaga tcggcaacaa cccatatttc aatacttacc tttatgaaag cggaactgaa
    15541 gcccgtggca tgtctggggt ggctatacgc ttaaaaaaca aaccctggaa cgtggttttc
    15601 ttgcaggaac gaactacttt aatcaacctg ctccaggaac gcaaccggac atcctcgctg
    15661 attgcgattt tgctggctgg ctttgtgggc ttgattacgg ttatttttgc caggctgttc
    15721 agccggccta ttttgtcact gagggaaact gcggccaata ttgcagcggg tgatatcaat
    15781 gcccaggcaa ccgtcacaac tcgagatgaa attggcgcgc tgggcgagac gttcaacttg
    15841 atgacccaac aactgcgggg cttcattggt gaactggaaa accgtgtgaa tgaacgcacc
    15901 gttgaactgg cacgccagaa cgaatcgctg caactgcgca gccaacaact ccaaactgta
    15961 tctgatgtag cccggcaggt tgtatcagca cgggaattag agacgctttt gaatcaggtg
    16021 actgtgctga tcagcgagcg gttcaacttc taccacgtgg gtattttcct gattgaccag
    16081 aacaacgaat atgcactgct gcgagcggct aacagcgagg gcgggcaacg gatgcttgcg
    16141 cgtgggcaca agctgcgcgt gggtgaggtt ggtatggtgg gttacgtgac cggcgccggg
    16201 cagccccgca ttgccacaga tgttggtaaa gatgccgtgt ttttcaataa ccccgacctg
    16261 cccgaaacac gttcagaaat ggccctgccg ctaaaggttg gtgacgaagt gattggcgcg
    16321 ctggatgttc aaagcattgt gtcgaatgcc tttactgccg aagacatcag cctgttcagt
    16381 acactggcag accaggtggc aatcgccatt cgcaacaacc agctttattc agagacgatg
    16441 cgcgctctgc aggaatctca aaagctgcac caacagtatt tgcagcagga gtggggacga
    16501 gaggctggcg agcgcggcga tgtggcctac atctacacgc ctggtgggat ggtggtgcgt
    16561 ccttacatca atacgcctga ggtggaacgg gtttttgaga cccggcagcc ggtcatcaaa
    16621 caggctgagg caactgggga tcgctctgta atggccattc cggttatgct gcgcgacgag
    16681 gtaattggca ttattcacct gcaagaggct gagaatacgg tccgagaatg gagcaatgaa
    16741 gaggttgcaa cagtgcaatc tgtggctgac caggttgggc aagcgttgga aaacgcccgc
    16801 ctgtttgaac gcactgtgcg ccgcgctgag cgcgaacgca aagtgctgga tattaccagt
    16861 cgaatccgcg ccagcaacga cccgcagcaa atggttcaaa ttgcgctgga agagctgcag
    16921 cgcgcgctgc gggtttcgcg agctcaggta ttagttcaaa cacccccatc gctgtctgaa
    16981 gatcaagaag attcagatgg cgaatcacct gctgaataat tcaggaagcg aggtcagggt
    17041 atgtcgtaca taattgcagt tgcaaacgaa aaaggcggcg tggcaaaaac cacctccagc
    17101 atttcgctgg gtgcggcact ggttgaaagc ggcgctaaag ttttactggt agacctggat
    17161 tcccaggcga acttaaccct ggcgctgggg atggaacccg acaaaaacca gcgttcgctg
    17221 atcagtgtat tactggaagg ggttgcgctt tcatccatat ggcgagaaag cggcataccg
    17281 gggctgtttg ttgcaccctc caattatgag atgggggttg ctgaacggct gctgcccacc
    17341 cgtccgggtt atgaggtcac gctccgaaag gtgctgacta cccaggcaga agattatcaa
    17401 tttattgtgc tggattgccc gccgtttcta ggcgcattaa cactgaacgc cctgacagcg
    17461 gcaaacctct tgatgatacc gacgcaggct gaatatttct cgatttatgc aatccgcaat
    17521 atgatggggt tgatccgtcg ggtccgttcg caacacaacc ccgacctgac ataccggctg
    17581 ctgttaacga tgtttgatcg ccgtaaccgc attcatcgca cgctggcaga gcagcttcaa
    17641 tccacatttg gcaacggctt gcttgaaacc attgttgaaa cggataccaa gctgcgcgaa
    17701 agccccattg cgggcatgcc cattatttac cagttcccca agagccgagc cgcgatgcag
    17761 tatcgcgcat tggctcagga gatcatacaa tatgcccaag aaacaacagc acaaccgaat
    17821 aaataatcta ttcaccaatc tgggcgaaag tctgacaccg caggaccagt cggttccggg
    17881 cgggttacct ggatggacgt gggaatgcga tcttgacggg aagtatgtga aatgcggccc
    17941 agaggtgatg gcctgcctgg gtattcggcc ggcgagtttt attgggcagc cgctaagtac
    18001 gttttcaatt ccaactgaat attcggctcc gctgcggcaa atattgttag ctgatacatt
    18061 cccggcgaca gttgcaattt actatcagtc ggctgaaggg gcatttattc cggtgcggat
    18121 gcaggttcaa cgccgctatt ctgagacagg cgagattaat ggttacactg gcattagtga
    18181 agttttaagc gagatgcctg cccgagtgga cgattcaact gctatgtttg cgcccggcag
    18241 cgagccgagt accacgattt ttccagcgac atctgcaacc actgaaatcc ggggagtggc
    18301 catcagcgat ggacaggttt tgccggcgat cgatttttgg accgaggctg ggcgagaaag
    18361 ccagaaccag ggcgggctgg ttgctcattc accagcgccc aatcgaccag cttcgattgc
    18421 agtgcccttt acactgcggg atcaagccgg ggggattgtt gaactggttg atgatacccg
    18481 acgccgagcc tggacgcagg acgaacaatt gctggtgcaa gatgtagcca atcaattagc
    18541 acaggcactg gaaaacgccc ggttatacgc gacggttcaa caagagttgc gtcaaagagt
    18601 tcaggcgcag caagaaatta cccggcgtaa ccgcgacctg gcaaccatta accaaatcgg
    18661 gcagcagttg agccggctga caggtgctga agagatcttc aacctggccc acgatatgat
    18721 tggtgaagtg ctggataaca aaaacctggt aattgcagcg tatgacgcaa agcaaaacct
    18781 gatcagtttt ccagttgcag cgcagggtgg gcaaagaata cagattccag cgcgcccgct
    18841 gggacagtct gtggtagatc atattgtgcg tgcccgagaa cccctgctga ttggtcgagg
    18901 tgtgcgccgg attttgcaag cgaaggaaat tgagctacca gagccgttgc cggcgtccat
    18961 cctgggtgtg ccaatgaccg ccggcgaccg atcattgggc gcaattgtgc tacgggactt
    19021 tgaaagcgaa gacgcctata cgcaagttga ggctgaaatt ttatctacag tggcctctca
    19081 gattgccaat gcgctagaaa atgcgaacct gttccaggat gtccgaaatg cgctgcaaac
    19141 gatcgaaaac cgcgaacgct atcaatcggg cgttgcaaaa gctgtggcga cacttgctca
    19201 atttgggtca aggtctattg ttgaggtgct ggaacagctt ggaactgcgg atgaatgcga
    19261 gttggccttt tttgctcaac ccgaagcggg tgatttgccc aatcaatggg agctgattgc
    19321 cgattggtac accagcctga gctcaccctc gcttaaggcg acatcgatcc caattcagac
    19381 tcgatgggtc aatgaactgc gcgagaacgg atggctggtt cttcaagcag tggactgccc
    19441 ggttgatgaa cagaactata tctcttctga aggcttcaag tctttgctac tgctctcggt
    19501 gccgggcaag aaatttacac ctggctttat tgcttttgga gagcgaaata aagaacgcac
    19561 ctggttgaac gaagaaatca atgcgttgcg agcgggtgca gacggtttgg caaacacatt
    19621 tgttcgcgaa gacctggtcc aacaaattca agacacgctg aacgaaactg aaagccttta
    19681 caacatcagt caccgcctgg cgctggcaaa tacgtttgat gaaatgatcg aggcgattac
    19741 actgggcttc cgtatcccca cgattaagcg cggcaaactg gcgatttttg cgcgggacgc
    19801 ggccgataac attataggtg ttgatgtggt gggtagttgg ccaagtttag atctgaatct
    19861 tgaccaagag attggatata ctgacccatc ttaccgaaaa ttctttgcca ttcaaacacc
    19921 gctgttcctc gactcgattg agcaggcaga ggtgcccatc tccattcagg ctgacctgcg
    19981 ttcacgctcg gttcagactc tagctgtctt gccgctctgg acgagcaagc gccagcttgg
    20041 tgtgctggtt ttggaaggcg acgaagctta taactttaca aaccgtgaaa tccggtcgct
    20101 gccgcctttg gttgatcaga tggcgacttc agttgagaac cggcagttgt ttgaacagac
    20161 ggaagaagtg cttgccgaaa cagagctgct gtataagatt tccagcggta ttgctcaggc
    20221 agtcaatctg gatgatctgg ctaagatggt gtcgcagacg gtgatgccac gccatgctga
    20281 acgaatttcg ctgatgacag tgaacagcac aggcgacggt gaactgttgg atattgaaat
    20341 tgcagggttt tacgataagc aaggagaatt tcaacggctg ggggtcaagg tacctgtggc
    20401 taacctgccc atcactcgcc tgatgacaaa tgatccgctg gtgatcgcaa acgcaaccga
    20461 agacagcatg gattcggttt cgcggcggac tttgcagcaa ttaaatattg ggtctggctg
    20521 tctggtacca ttacgcagtt caggccactt gatcggtata ttatctgcct catcacgcaa
    20581 gccggcagaa tacagctctg aagaagtcag gattatgcag gttaccggta atggtgttgc
    20641 tgtagcgctg gaaaggcagc gcttgctgcg agaggcacag cgccgagcgc tcgaacttca
    20701 gactgcggca gaaatcgcgc gcgacacaac cagcacacta tccctggata cactactgga
    20761 atccgtggtc aacctgctgc ttgaccggtt tgacttctat tttgcctcgg tgttcttgct
    20821 ggatgaaacg ggaaattacg cggtcattcg agaggcaact ggcgacgctg gacgcgagtt
    20881 aaaggatagc gattatcatc tggcggtggg ctcacgatca gtggtaggtt cagtaaccag
    20941 cagtggtacg ccattggtta ttaatgacgt cagcaagagc ccattgtatt acccccaccc
    21001 cctattgccc gaaacacgct ctgagatggc gctgccgctg cgcggcgccg gtaaagtgat
    21061 cggcgctctg gatttgcaat ccaaccgggt gaatgcgttc tctcaggatg aaattgcggt
    21121 cttccagatt ctggcagacc agttggccat tgcaattgag aacgcccgag cttaccagct
    21181 ttcacaagaa gcttttgaaa acatccgcga aatggaccgc ctgaagagcc agttcctggc
    21241 gaatatgagc cacgaactgc ggacgccgtt aaactcgatt attggcttct cgcgcgtgat
    21301 cctcaagggt attgatgggc cgattaatga cacgcaacgg caagacctgt cggcgattta
    21361 caactccggg cagcacttgc tggcgctgat taacgacgtt ttggacctgt ccaagattga
    21421 ggcaggcaaa atggaactgg cgttctcgga agtcaacgtt ggcgatgtta tagtcagtgc
    21481 catgtctacg gcagtggggt tgacgaagga caagcccatt caattgcggc atgtcatacc
    21541 cccgaatctg cccccagttc aggcggattc gacccgcata cggcaggtct taatcaactt
    21601 tatctccaac gcggccaagt tcaccgagga aggctcaatc acggttgagg ctatgctctc
    21661 aaacggcccg gatgggtcac ctgagattat ggttaccgtt accgatacgg gccctggtat
    21721 tgctgaaaaa gatcagatta aattgttcca agccttctcg caggtggatg actcaccgac
    21781 ccggaagacg ggtggtacag ggttgggtct gtctatcagc cgatctctta ttgaaatgca
    21841 caaaggccga attgggctac taaagtcagt agtaggtgaa ggcagcactt tcttcttcac
    21901 gctgccactg cccaaacctg aacccgaacc ggagcccact gacgacagct ctaacgttat
    21961 tctggcgatt gacgatgacc cgcaggtcat cagtctttat gagcgcttct tgcagcccca
    22021 ggggtatcag gtaattgcgt tgaccgaccc caccaaagca gtggaacggg cgcgcgaagt
    22081 aaagccgttt gcgatcacgc tggatattat gatgccagaa cgggatggct ggcaggttat
    22141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgtttgcgat cacgctggat
    22261 attatgatgc cagaacggga tggctggcag gttatccatg acctgaaaag caaccctgag
    22321 acacattcaa tcccggtgat tatttgcagc atcgttgaag atgaggaaaa gggcttcagc
    22381 ctgggcgccg cggattatct ggtaaagcca ttcctgcaag aggatttaac caatgccatt
    22441 atccggttga acgccgatgg agacattcat cgagtgatgg tgattgacga tgacccggat
    22501 gatctgcggt tggttcaaaa aatgattgag gacggcgggg atttccaggc tattctggca
    22561 gaaagcggcg aacgcgggct ggaattactg gagactatgc gcccggatgc gattattctc
    22621 gacctgttta tgcccggttt gaatggcttt gctgtgctcg aaaagctgcg ttcggatgcg
    22681 aagctgagca gcatcccggt gattatcctc acaggagcgg atctgacagc tgagcagcat
    22741 aaacaattat cggaatttgg taagcggaca ctgaccaagg gcatgctgcg tgagcgtgaa
    22801 ttgatcacat ccctcgaaga atcgctgcgc aaacttaaaa atcaacgacg agagtaacga
    22861 taacctatgt cttcacaatt tattgttgaa tgtattgact gccataatcg cgggcctttt
    22921 tttgtaaaca gcaccgcctg cccaaactgc ggaagcgcct ggcgcgaagc gcgctacgat
    22981 tacgagacga ttgccaagac actgcccctg gtgctgcatg agcgtccatt tgacctgtgg
    23041 cgatatcgcg agttgctgcc ggttcgcagt ccaaacccag atttgtcgct gggcgaaggc
    23101 ggcacgccgt tgattcgcgc aaccaatttg gggatgatgc tcggctgccc gaacctgttt
    23161 attaaggatg agcggcaaag cccaacccat tcttttaagg atcgacaagc cgctgtgaca
    23221 gtggcggcat taaaagaggc cggggtaacg gaagttgtaa tcgcctcgac tggaaacgtg
    23281 gcgatatcgt attctgcata tgcggcgcgg gcgggcatca agtgctgggc ttttctcacc
    23341 agcctggtcc cgggcgccaa gatgcgggaa gtcgccatct acgggacgca ggtggtgaaa
    23401 attaccgggt cttacgatca ggccaaacag gtagccaata cttttgccaa gcagcgtggg
    23461 ctgtatcagg ataccggagc gcgcagcatt cccaatattg aggcaatgaa aactattgct
    23521 tatgaaattg tggaacaact aaccactctg tttggccccc caacgggcag cggtgatcag
    23581 ggaatgccca tctggactgc gcccgactgg tatattcagg cggtcagcgg tggcatgggg
    23641 ccgctggggg tgattaaggg cttcaatgag ttattccaaa tgggattgat tgaccgggta
    23701 cccaaaattg cggctatcca agccgatggg tgtgcgccca tggtgcacgc ctggagattg
    23761 gggcaggaaa cagccacacc cgttcagtca ccaaatactc agattatcac cctggccacg
    23821 ggtgatcctg gcagaaccta taccttgctc cgacagcgca tgctggcagc atctggagga
    23881 acctttgaaa gtgtgagcga tgaggatgct taccgagcga tgcacctgat tgccaaaatg
    23941 gaagggcttt cggttgaacc tgcggctggc gtggcctttg cgggtttgat taaattaatc
    24001 cgcaatggac aaatcaaacc cagcgatcgg attgtggtaa attgtacagg gcacacgatg
    24061 acgatcgaga agaatattct cggcgagggt tggtctcgca atgtggttct ttctacgcag
    24121 gcaatggaag acagcccgga agatggtctg ctggcagcgt tgagccgggt ggcaattgac
    24181 cggttcccac gaattgccat tgtggatgat aacccggatg tgcggcggtt gatccgccgg
    24241 attctacaat ctcaggggaa ttatgagctg tttgaagcca ccaacggacg tgaagcaatt
    24301 gagctggcaa aacgagagca gcccaatctc attattctgg atctgatgat gccagaagtg
    24361 gatggatttg gtgtgttgca agcgctggaa gccgatccag ataccgctga gattccggta
    24421 attgttgtga ctgcaaagga gttgacccct gccgagaagg cgcgcctcaa aggacatatc
    24481 caaacattaa tgcaaaaggg ggattttatg agtgatgacc tgcaagatga ggtcagggca
    24541 ttgcttgggt aaatcaagct aaattgccat gaacagcaaa cagcgcaaaa caggaatatc
    24601 agccgcactt gcttcggctc tatttctggg cgcggtgccg atctttggca agcaggcgat
    24661 tctatttggt tttgcgcctc tggctgttgt ggcattgagg acatctattg cggcggccct
    24721 gctgacaatc ttgatgttga tatttcgacg gcagttcttt tatatcttcc cggtggggtt
    24781 gattggctgc acggtggcag gagtggtcaa cggtctgggg tccattctat actacacctc
    24841 actgcaaact ctggatgcag gcgtggggca attgctttac gcgttttatc ccatttttgt
    24901 ggctttctgg ttgatgctag accggcagcc gatcagtcga ttaaccattt tccggatggt
    24961 gctctctgtg cctggtattt acctgctgat tcgagccggg cgcgatggtg taaatttgct
    25021 gggggctggg atgatgctgg gatcagccat cctttatgcg ctgcacctga tcatcaatca
    25081 acgtgtgctg tatgaagtac cagccccaac ggtgacactt tatacgctct acgccatggc
    25141 cgcaacagtt gtgattgcct acctgttatt taaccgaaca ctgccccccc cttccacgcc
    25201 ctggtggccg attgtgggca tgggtatatt aacgtttgcg gctcgcctga cattgtttac
    25261 tggggttaag cacgttggcg gattacagac cgccatgctg ggctttttag agctggtgat
    25321 caccgtgagt cttgctcata tatggctaca cgaaactctg acacccagtc aatggattgg
    25381 gacggggctg ctggctgcca gtctaattct ggttgggttt gataagttca cacccgaaaa
    25441 gcggcgcaca accgggctgc tggcctggct caatccgccg gggattccga cgttcaagct
    25501 gccctggcaa tagcacaatg tggttaacca cctgagctat tgttaagcgg gcacatcaaa
    25561 ccgaaaattt ttgagctgtt ctttggctgc tgtatagcca acttcaataa tttgcagata
    25621 ttgatcaaat tcgagcaggc caaatttttc aaccgggacg cggatgatca ggtcggcgca
    25681 atgccctgtt tcgtttaaac gataacggtt gttgctatat acaatcccat ctacgatatt
    25741 gagaaggttg ggagatttaa tcttgccgcc aaatgggttt aattttgaga ataaagcctg
    25801 ccaacccgaa aggctggggc caaagttata ttgtccttta acggggctgc tggtcagcaa
    25861 atcaatgcca atcaccgttc cagtggggca aaattcacgc atacgatcta ccggcatgtt
    25921 attggccgcg ccgccatcga ttaatacaca accattttga tccaggatgg gggcaaaaac
    25981 ccctggaaaa gccatgctgg atcgcattgc gcgccagatt gggccgcggg tatgcgattg
    26041 ttcttcgccg cgggtcagat tggcagagac acaaaaatat gggcgccagg cgtcttcgat
    26101 catggcctcg ccacacaaag tttgcagcaa ggtggtgata cggcgggtgg cggttattga
    26161 ggtgtacggg aaagtgtaat cgagcagttt tttaggatcg gagaacttct ctgccagttg
    26221 gcgcaatacg tctggggacc aatccagggc gtagccgcca gcaatgatgg cgcccatgct
    26281 ggctccaccc acccagtcca cttcaatatc tgcttcagcc agggcctgaa ggacgccaat
    26341 gtgagcccag ccacgcgcac cgccgccgct caatgtaagc gcaatggggt tcccgctcat
    26401 ccgacggcat aaacgacgaa gatcagcagg gttgtcatgg cggatgtggt ggtgatctcg
    26461 aaccggtagg tttcccaaat ggcgcttttc cagccaatcg gttgtgccag aaggcacttt
    26521 gcaatccggg cgatgcatca ataccaactc tacacgagcc cgggtgctgg cggatggcaa
    26581 aaccatttcc actgctgaga tctcgggcct ggcatcatcg tcggtgacta acaagatcag
    26641 gtcggcatct tcaacacagc gctgcgccca gggggtcagg tggctgtctg catccaaagc
    26701 aggtcccaca tcataaagag catactggtg acgccgttca cgctcatcca gccaggtatc
    26761 tagcaacaga ctggtcgggt gatctaacgg ggtctgagag gccccgggtt taccataaag
    26821 cgagtcgaag cgcctggaat ctaaaaaaag cgttgtcccc agcatgccca ttgtctcagc
    26881 aagttggctg gcgaatttgc tgcccagtcc gtccggctga agcggcacca cagcaataac
    26941 ctgggcgctg actggagccg ccacagtctt tgagaccctg agctcacgct gaataatccg
    27001 gcgcgacaat ttgagcaaga cctgagaaaa ctgacacagc aggtcctcaa aaacctggcg
    27061 gctgattacg atgatgtcgg tcaagcgagt ggcgtagatt gtggcgcttc ggcggctttc
    27121 aagggaaccc ttttcggtga gcaaggcgaa ctcgccgact gcctctcccg cccccacttc
    27181 gcccaggtca tgcatttgac cttgatcttc agaagcaaat cgcaaacgcc cctggactat
    27241 gatgtacatt tcatttccgg gctcgccctg gcggcacagc acttgtccgc tatccagacg
    27301 gcgccattcc agatgtgata gcagctctga catggcggct tcgtcaagcg ggccaaatac
    27361 acgggttaag gcaagctgcg cctgaacctg ccggacgcgt ggcagcaggg cacaagccaa
    27421 ttcattaacc atgcctgggt gaacggcacc cagtttatca aaagcctgtc gagataagtg
    27481 caagagaagc gcatcccctt cagctatgac agtaaattcg tgcggttgac cggtaagcaa
    27541 agacatctct ccggtgcaat cgcccgggtt gagcacaccc atgggcacat ctgggctgtc
    27601 gggctgaggc aggtaaattc tcaaacggcc agatttgaca acaaaaagcg aatcgcctgg
    27661 atcggcctgc cgaaacagcg tctgacctgc aggcagtgga tactcctgca tttcgtttgc
    27721 tatggctgcg gcggctaaag gatcaagctg gcaaaaaatt ggaagttgtt tgatgaaatc
    27781 aagattgtcc attttagtct cgatcatttt gaaatttacg gaagctgggg gagaataata
    27841 atctatgtga ttagtgtact tatctcttac gacctgtcaa cagaatttac gccaggaaac
    27901 aatcggagta aaataggcca aagagttcag ccattactca gcaaggttca aagtatgaag
    27961 attactggta ggccacaaaa tcaatggggt tgggtgcgac tgattagcgc aatcattctg
    28021 gctggcgttt tcttctggac actgctggcg gttattctca aggcatttcc catttcctgg
    28081 caggttgttc aggattattc aatcatcaat ggggtggaac tggcgatcat ccccttgctg
    28141 cttacgctgt ttgcggggtg gatggaagag caagacgtaa aagcagccaa tgaaaaagcc
    28201 agccaccagg aaaccgaaca tcttttaaca atgcgccgaa aggctgtgct aaaacatttt
    28261 tttgagacga ccaacgcaat tttggcggaa gatcaccagt tggaacatga tagagagaag
    28321 ttacaggata ctgttcagtt aacactcccc gaacttgatg ggaaagggaa gggggaaatt
    28381 ctgatttttc tgcatgaaaa gggcctgctg agtggggagc aatctattga tttacacaac
    28441 gccgatttta gggaggttga attacacaga gcgcatctca atggcataac actagaaagg
    28501 attgacctgt ctcgggcgcg gctggaggga gcacatctgg ctggcgggaa gctgcacggg
    28561 gcgaatctgg caaaagcctg gctgcgccac agtaatctac agcgagccat gctgaccggg
    28621 tgcaatctgc gcagtgctca tttagaaggc gccaatcttg agggggcgga tttaagccat
    28681 gcctgtcttg tagatgcttt tttcaagaaa gcaaatctgc gccactgcac cgggcttagt
    28741 atctctgatc aacaccctga tgaaggcgcg gcgcgccaca atcaaagtgt gggcgctgct
    28801 gatctggagc aggcaattct cattgatgcc attctgccag atgggcagaa atttaccaat
    28861 gaaaaagggc gggctttttt gcgtagaaaa gagatagaag aactgattga ccggttatag
    28921 acagacgtta cccgccaatg tgaaatatgc cgcagccagg ccagcgagtc tgtgcggcct
    28981 ccatcagttc agggcggatg accaccagcg ccaggcgata gcggaacgag tcgagcgcca
    29041 ccaagccgct caaccagcct ttggcgtgga taaattccaa tgggcctaat tcatctacaa
    29101 tcaacaaatc acagggggtt gctgcgcgca ggcagctatc gccccaggcg agagtcagat
    29161 catcaaaagc ccaatggcct gtctgcgtgg cggagtgagg gtttgtttcg ataagctgag
    29221 ccaaaggacg ggattgattg ctgcgtaaat ccaaggcgtc aataccgatt ttcagagtac
    29281 cctcaaaacg agcaggagaa agcattccgg caacatcaaa gccgcgctgc tgcatgcgcc
    29341 gggcaaccaa agtgcaaaag cgggttttgc cagtgccacg gttgcccgta atgaggatta
    29401 atcgtcccat tgcaataacc gcttgccatc caattggcgg gtgcgcagcg agatgccata
    29461 cgccgtacta agatcgtcat cattccaaac ttcagccaac ggtccggcac gcagaacccg
    29521 cccggagcgc attaagatta gttgagcggc taatgccgcc gccacatcgg gatcgtgagt
    29581 cgtcagcaaa attgtgacac cttcggcagc caggctgcgg agctggcgga ccaaaagttg
    29641 tttgttcgat agatcaagat gggcggtggg ttcatcaaga agcagcagag cgggctgttg
    29701 agccagtgcg cgggcaatta acaacaattg atgttcgcca ccgctgaggg tatgaataga
    29761 ctggtgggcg cggtcagcca gacccacgcg ctccagggcg gtttgcgcaa tctggcagtc
    29821 tgttgcgccg gggagttcca aaggatgcaa gtgcggcgca cggccgagta aggcatactc
    29881 cagcagagaa tattcgaagg gattgtactc gcgctgcggc accagcccta cacgctggcc
    29941 gatctgccga tgggtgtact gggccagagg agcaccttgc aagacaatct gaccagaggc
    30001 gggatgcagt cggccataag ctaaacgcaa gagggtggtt ttgccagcgc cattgggccc
    30061 caggatggcg gttgtctctc cggggatcag cgtgagcgag aggtcatcca gagcaggatg
    30121 gtcggcctga ggataggaat acgttactgc gtcaaaagcc aggacagggt tcatcaggat
    30181 tggggttgta acaatgggcg ggtgagcaag gttagaaacg ctactgcgcc aagaagggag
    30241 gttaagatgc ccaggggaat ttcgccgacc aacagcaggc gagccagatt atcacaaaca
    30301 accaggtaaa tggcaccaat caacaaggcg cccgggagag catagcgggc atctgcccca
    30361 aacaggcggc gggcgagatg cggtgtgatc agcccggccc aaccaatgag cccgccaatg
    30421 gaaatggacg cggcagtggc ggctaccgcc gcaagcagaa ccgccagacg ctccgaactg
    30481 ccagaagcgc ccagagcagc ggcggcgtct tcgtccagcg tcagcaggtt gagacgccag
    30541 cgcagaaaga gcattacaac cagcccgccg agcatcaatg ggagagctgc gagaagccgc
    30601 ggccaggtaa cgccatataa accacccagc agccagaatg taatttcggg caactgacgc
    30661 agtggatcag ccagatattt gatcatgcca acgccggctg agaacaacgc cgagacggcg
    30721 ataccgctca gcaccaggcg caagacccag ccaccgtaac ggacgcgccg ggcgagcagg
    30781 taggatgccc ccaaccccag caggccaaaa aacacagccc cgccctgcac cagccaggct
    30841 gcgctgccca gggcgatgat ggccagtgct gcgccaaagg ctgccgcctg cgaaacacca
    30901 agaaaccctg gttctacaag cgggttggaa aacaccatct gaaatacaac accagccgca
    30961 gccaggctca tgccaatcag tacagcggca atcaagcgcg gcaggcgcaa gttccagact
    31021 aactgctgcg ccagagggtc atccaacaac cgcgcggggg gcattattcc cagattggga
    31081 taacgcccca gcaccagcga gagagcgagc agagacagca acaacacccc caacagcgcc
    31141 aaggcgcggc gggctgcccg gggagtgcgc gccaggggat gcataatgat tatggctcca
    31201 gatttttcgg caagaccggg gcaatcagtt gttcaaatgc agcttgatcc aggccgtaaa
    31261 gtttttggta aaacgtctgg gtttctgcga gcatatcaac aggcagtacg cctggctgaa
    31321 ttgtgtcggc cagccagagt aatcccaacg cccagcgggg atcgggttgg tcccagctca
    31381 ggtaatccac cgggaaggga tggagctggc ccgctttgac tgcgcgcagt tcctgccagg
    31441 cgggatcttg ttcaagctgc gagacaacat cggccgcatt gacagtatag gcgaccagat
    31501 agatttgatc ggcatcccag gcggcaattt gctccagggt aatttttgtc cagccagagc
    31561 ccagttcaat atcctgccag atgggttcgc cgcctgcgag ttgaaccagg gtggtctgca
    31621 gccagccaac tggcggcacg ttgaaggcaa cttcgccatc gcggttggta tagtagatca
    31681 gcaagacccg ggggcggggt tggctctggg cgagcccggt atttacgcgc tgaacgacat
    31741 cggcataata ggtggttaat tcagctgcgc gggcttcatt ttgaaagacc tgacccagaa
    31801 tgttcaagtc acgcgtgtac tgttcggggg tttccaaatc cagatagatg accggaatgc
    31861 ccagggcttc caggggggtg cccagttttt cggccatgta gcttttcatg atcaccagat
    31921 cgggggtgaa agcaaccacc tgctccgggc ctacgtctgt gccaaaagcg gctttatcgg
    31981 caaaatttgg atccagaact gagataaaat ccccagtccc ctggctggtg gttgagaaag
    32041 ccaccacccg gtcctcagcc tcggggaaca gatagacagc atcggcgagc aaggcgctgg
    32101 cgcgcccggc gatgacaatg cgctgggggg cggcgggcaa ggtgatggtg cggccttgag
    32161 catcaacaat cgtgatggat gcgttggccg gagcaggcgt gggtgtacaa gcggacagag
    32221 ccaaaacaaa gactaacatg aggagggtga tatatttttt cattgtgtgt tttccttaac
    32281 agtgatgggt atcttatcac gaacaacagg tcgaatacaa ggctggcagt tactgagata
    32341 tcaacttatc taaatgttca tctaaatacc ccatttgcaa gtgaagataa caaatctgta
    32401 agctgaaatt cgttcacctg ttaactaagc agcgttataa tgtactggca tttggttaac
    32461 gtgttaacga atttacacat attatttccc gagttgaccg ggtggcaccc gggaagcatt
    32521 ctaattaccc cgggtactga taaggagggt ttaaataatg gctgaaatac gagatatctg
    32581 gttgcacgcc caccatatga ttcgatctgc ccggcagata atcaacgaaa atctacgctc
    32641 gctgaacctg agcagcgctg aggggaatat cctgctccat ttgttgacgc aagaacagga
    32701 gatgggtcag gagcaattgg tcgagcagtt agacatcagc aaaccggcag tttcgcgcgc
    32761 tctcaattcg ctggtggcaa agggttatgc tacgtggctg ccagacccgc aggaccgacg
    32821 cgctcaccgg gtgcggctga cggagcaagc gcagaaaatt ggtccggaca tcgaacgggc
    32881 ctataaccag atctatgctt tggcaatgcg gggtatctct caaaaagaat tagatgattt
    32941 tgtaaaacta tttgggcgga tatctgagaa ttttaggtgc gcacaggcaa agaaaataac
    33001 agaggagaat tatgctgcac aacgattggg ttagcatcgg tttgtacttg ggtttcattg
    33061 ttgtatttgg gctaccggca attctgctca aagtgggatt caatgtgccc tttgagatat
    33121 tgcgtaaaat gtaccacatg gtgattgctc tatcgatctt tcccttggtg atgtttttca
    33181 aaaactggta catggctgtg ttatcggcct ttttgctcgt ggttatcgcg tatccgctac
    33241 ttgcactagt ggaaaatact gccatataca aacggattgc agtagagcga tcaagcggtg
    33301 aatttaaaaa gagccttatt gttgtgcagg tgtccatcgc cctgttgatc tttattttct
    33361 ggggcttgtt ggggtcagaa tggaaatata ttgctgttgt agcagtaatg gcctggagtt
    33421 ttggcgacgc tgccgcggct ttggtaggca aaagatttgg gcgtcgccag atcaggcatc
    33481 cccggattga gggggcaaaa acgatggagg ggacccaggc aatgtatttc acagccgggc
    33541 tggctatctt cctgacattt atgttctacg ccggccagcc ctggcaggta agtttggctg
    33601 ttgccctgct ggttgcaccg attagcgcgg ttgtggagct gttctccaac cgggggatgg
    33661 atacactgac cgtgcctatt tcaactggtc ttgccgttct atcccttatg tcactttttt
    33721 ctctcttggg aacataaaca gtgaatgcgc tttcaaaaga acaatctagc aatcgcgaaa
    33781 agacactggg gatctccctg ttgttgagtg ctccaggccc cctggtgacg ggcattgctc
    33841 tgattagcag ccattccact acccaacttg ctgatttcct ccggcgcggg gtggaactgg
    33901 tggcaatctt tatttcctgg tgggttttcc gccgcctgca tcaaaatggg gcagtggatg
    33961 gtgttcagca agctcgactt gagcgtacag cagggttgag cactgccgga gcgatggctt
    34021 tttcgggagt gattctactg ggggtgactg tatcgcggct gtcggcattt gagcctggtg
    34081 gcaatgtcat ctcggggctg gttatcgctg tgttaggttt ggtgaccaat ggttggtttt
    34141 ggaggcgtta tacgatctta acgcgcgaac aatacagtgc tgtcattgca gcgcaacagc
    34201 agttgtaccg agccaagaca acggttgatc tctgcgtaac tgcagcgctg atggccgtag
    34261 cgattgcacc caaccacccc atgacgcgct atattgacct tctggggtcc attatggttg
    34321 cagtttacct tttatggagt gggctgcgag cggcacaaaa gcatcctatt atccgacagg
    34381 ctaaattatg aaaataagcg caacaatgtc ccctgagatg cggcaaattg ccagaagaat
    34441 gccaaagttc tcgataaatt cgagaagcgt tcggttttcg cggcgcatta tgcggtggat
    34501 gaccaggccg ccccagtggg ttttggaagt ctttaccagg ctgctaatga agtctccttt
    34561 gaacttgtct gatattcaga tcagggatat tttcattgac ggtcaagatg gggctcaaat
    34621 ccggttacgg gtttatcaac ccgggacgcc gccagtcaaa gcgccggcgc tgctgtggat
    34681 gcatggcggt gggtatgtca tgggcatgcc tgagcaagat gatatttatg tcctgccgtt
    34741 tgttcgggag gttgggctgg ttgttgtttc ggtggattat cggcttgccc ctgattatcc
    34801 ctttccgacc ccgctcgaag attgctacgc cgcgctacaa tggatgaccg ctcaagcaga
    34861 gatacctggc attgacccga atcggattgc cgtcggtggg agcagcgctg gtgccgggct
    34921 ggccgcggct ctggctcagg tggctcacga tcgcggtgaa atccatccag ttctccaact
    34981 tctggtttac cccatgctgg atgaccgcac tggcggcctg aaggacattg aaccgaaagc
    35041 gcacgttgtc tggaacaaca ccagcaacca gttcggatgg gaatcgtatc tcggccagtc
    35101 atgcggggca gagaccgcac cggctggttc tgtacccgcg cggcgggaca accttgccgg
    35161 gctaccccca gcctggattg gcgtcggtac caatgatcta ttctacgatg aggcagccgc
    35221 ctatgcccgg cggctcagtg aggccggcgt gccctgcgag ctggtgcccg tccccggagc
    35281 tttccacgcc tttgatctat ttgctcctaa aatgcaggct gtcagagatt tccggcaggc
    35341 tcaagtagtg gcgttgaaaa aagcccttta tggaaatgga taaaccgcat ctcgaaaaaa
    35401 caattgcaca tagaacaaga ggaaattgtg aaataccatc cagtaaaaat catcggtgca
    35461 gttcttatat gtgtaattct ggcaattttt gcagggagaa cattaaaagc attcataaaa
    35521 attatcattg tccgtgtcca gtattgatca gatatagttt gcgctgccaa taaagatgcc
    35581 ggaacggcaa ccagcctgta taatattacc tagatttggt tgtgatcagg acagagacca
    35641 tgttgagcat cgaacaaatt cgccattatc catttttcaa ggggctaagc gaagacgaac
    35701 tacaaatgct ggcaggttgc atgaccaagc ggtgttttaa taaaaatgct tacatttacc
    35761 atcagggaaa cccggcaata aatttatatc tgatcgagtc gggcatggtg cggctttttt
    35821 tctgcaatga taccggcaac gaatttatta tgaatttggt agggccaggt tcggtgattg
    35881 gccattcatt agcaatgaac aacc
//
LOCUS       DAOPVS010000011        35658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1130_length_35658_cov_54.845715, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000011 DAOPVS010000000
VERSION     DAOPVS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35658
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1130_length_35658_cov_54.845715"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKL11_02275"
     CDS             <1..458
                     /locus_tag="PKL11_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HNZ12454.1"
                     /translation="PAETVNQLGLLGFSGIANVLSAIKFAKYYELGETDVVLTVLTDS
                     MELYHSRIEEMHAEFGAYTELNAAADFARHLLGVTTDNLLELSFVERRRVHDLKYYTW
                     VEQQGKTYEEILAQWYDPTYWTDLHGQAAEIDALITEFNERVGLIQKYR"
     gene            464..1030
                     /locus_tag="PKL11_02280"
     CDS             464..1030
                     /locus_tag="PKL11_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12455.1"
                     /translation="MDAWPPSTALILVGVMPSPRDLEIARVLGWYRIPLRTAPKVVAV
                     DYLAFYQTAAFGEDHGARIECVAPVRGHELTTRAELLRAEPDHPRAHEEYYKLLLGPL
                     QTLPRPITSGAWRRLTFLYTTGELLQSAQTLDDLVVRSDERALLWASLRERAAQAGQY
                     HAADLPELPLDPAILLMLGGLGDMDPGN"
     gene            1053..1943
                     /locus_tag="PKL11_02285"
     CDS             1053..1943
                     /locus_tag="PKL11_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNZ12456.1"
                     /translation="MSNKNNSGKKTREPQLAYNVMTLAPIVKARPFVKWAGGKSQLLP
                     AYVTYLPSLSQIQRYFEPFLGGAAVFFYLQPEQAFLSDSNAELINLYSVVQDRVEELI
                     VELTAYQNDEDFYYAVRSKDPGSLTPIQNAARFIYLNKTCYNGLYRVNSSGQFNVPFG
                     RYVNPTICDAEVLRSASLALSGVSLTVADFEKAVSSASARDFIYFDPPYHPVTKTSSF
                     TSYTMGKFGDDEQHRLARIFKELDQKGCLVMLSNSDTPLIRELYKAYKIIEIQANRAI
                     NSNPEKRGKITELLIINYGR"
     gene            1947..3209
                     /locus_tag="PKL11_02290"
     CDS             1947..3209
                     /locus_tag="PKL11_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12457.1"
                     /translation="MVSKNDYAWKTYISKNNLSLDGQPYRVIAKQLREQTGREPRLMA
                     KFDEPAQVSKPLSEQNYTLLAVTNLEYVLFQGTTFTEVPACTDARKVSASLPFSLVTA
                     GRGTGESEYLDNAFNSGILQDFSGCDPLFLTIRGKERSRNFDFYINNAQLSLQTDGVQ
                     IEVDGGYEGERDVILVEAKIGNRKHFNIRQLYYPYRHFSLIAPQKRIRPILFLYDLLS
                     ASYTFYEYKFSSQNIFDSIELVRCCRYNLTPGKGLRLSDLIDVGFETTSDLIPQADDF
                     NKVLELLTVINSGLDTAQDIADYFGFEPRQSYYYAEAAKFLGLICDEGFQLTERGTQF
                     LRTPPKDQQLLAAKLLINSELFRRLISVARRKGYYAKDDVVQAILDVGKYNLTTSNRR
                     KQTVMSWSNWLANQIGCFREQEGKYFIN"
     gene            3252..4670
                     /gene="hydA"
                     /locus_tag="PKL11_02295"
     CDS             3252..4670
                     /gene="hydA"
                     /locus_tag="PKL11_02295"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNZ12458.1"
                     /translation="MRKLLIRNGTLVTAAELYPADILVIGEQIAAIGQNLPADDAEVV
                     DAAGKLILPGGVDVHTHFDLPMFGTVSSDDHYTGHKAAAFGGTTTVIDFVSQDFPSLS
                     DSIAAWHAKADPKAAVDFGFHMNITHLDAAIEAEMADLPGLGITSVKVFTAYNNRLRL
                     SDGDIFRVLRLGAQHGLLTLMHAENGDVIDLLTAEALAAGYTAPEWHAYTRPSWGAVE
                     ASLRGAAFAAQADAPLYIVHMNTGGEVDMLQYARQKGLPVMGETCPQYLFFTVNDLRR
                     PDGAKWVCSPPMRTAMDNTRLWQGLMDGTIQVIATDHCPFFFDGTQPITYEGQPIAIP
                     GKELGQSDFTKIPNGLPGVGDRLPVLWTHGVLRSRLTPSQFVALNCTNPARIFGLYPR
                     KGSLLPGADADIVIWDPALTTLYGVAQAQHRTDYNLYEGWELTGMPAQVYLRGQLIVD
                     GSRWLGAAGRGHFVPRGAPEII"
     gene            4689..6275
                     /locus_tag="PKL11_02300"
     CDS             4689..6275
                     /locus_tag="PKL11_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12459.1"
                     /translation="MLVLLPALLLIVLGFALLVIQRMRPNFGYAWLGASASTLIVWGL
                     MLYLRFRLPSELVIENWRAFSALGSDSLVFQVDYISWPYALALSTLIAAVVISSPARL
                     DAGGSLPSWDRGLMLVGMGLLAVFAASPLALVLMWTAIDVVELLALLGTASGDRLERS
                     AVTSFAFRVTGTLLVLLAVLQGGPGATFDFISHSQHLSLLLVLGAGLRLGVLPLNVPY
                     ASEPVLRRGIGTLLRMVAAASSLVLLARLPENAVPAAWVPWLLILAVLAALYGAGRWA
                     AAKDALSGRPYWLVAMAALAIGCVAQGQPSASLAWGVAMILPGSVLFLFSARSTGLLA
                     LPALGLIGLLGLPFTPAAGGWVGLLGSPINGNGLLFLVAQALLAIGYIRHALAPARPW
                     SELETWARAVYPLGLALLIVADILIGIWGGPGFRTLGIWWAAVISTAFIGFLVIGGRL
                     LRPAARVGWLVTLGQRVVNFLSKIISLTWFYRLLEWFYARLGQVVRFLSLIFEGDGGV
                     LWTLVFLALLITLVRSQVTP"
     assembly_gap    6369..6468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6642..8039
                     /locus_tag="PKL11_02305"
     CDS             6642..8039
                     /locus_tag="PKL11_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNZ12460.1"
                     /translation="MSAPLIWIVIPLVLTGLLWLLHRQPKRAALAMLIICLVLVILAA
                     ALPIGSFIKIGRTTFELPTTLTLFGRRLVLEASDRIWLMLVYGLGAAWSLGIVQSRVH
                     RSFTPLSLLMIVLLVAARAVEPFLYAALLVAVAVLASLPLMLPPDARPGRGIMRYLIF
                     QTLAVPFILLAGWAASVVDANPSNEVLRLVASASLGLGLAFWLAVFPFYSWVPLLAGE
                     AHPFAAGFVLSLLPTTALFLGLDFLNGFGWLRNSEIILTALRLVGVLMVVTGGIWAAF
                     QEDLTRLFGYAIIVDLGAALLSISLRSDIGLTIFAAGLIPRLLSLFIWALALAFLRVR
                     QIPLTFAGVTGLGRRLPILSAALVLACLSLGGLPLLAVFPARQALLVELASQSSIAAA
                     WALAGSFGLLLGGFRLLNVLLKRTEPGWQVTENLSEGLLLSAGIIILLLIGIFPGGIL
                     GSLPDTLPLAFPNLP"
     gene            8064..9245
                     /locus_tag="PKL11_02310"
     CDS             8064..9245
                     /locus_tag="PKL11_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12461.1"
                     /translation="MDIEQFVARLEWLEDERRKDKLTISTLQERLASLESSLPGMVQQ
                     IKDLTGDLARLTASLARIDQVESAIAQVRVEFSRALDTSERTRAEHDREVDRARQADM
                     DVVNKSFAELRKSIESIGELRRGLQSRTEEDFRLSRLIEELEQKVTQTRRSDDEYRHA
                     QKLIEDGQRQEAKRVTDLQGEVAAIRKRMEEQRGKLDVTVDTLRKVETRLADFQTTEG
                     ERRQAQVAFIEKQNMLNVDRDRTWKEWQTRFEQIDSVSTNLDTQLQSLDATHRAIKRA
                     QEGFEEITQRFERRINEITEMQRLSEDRFRQEWVGFRADDQKRWTNYTLSQEEQQREA
                     GRQYDKLVERMTLIEESTHELNDALTQNAEQTLKRLQGLMALSREWLEMQEQNGNSLR
                     S"
     gene            9255..11138
                     /gene="selB"
                     /locus_tag="PKL11_02315"
     CDS             9255..11138
                     /gene="selB"
                     /locus_tag="PKL11_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNZ12462.1"
                     /translation="MHVIGTAGHVDHGKSTLIAALTGTHPDRLKEEREREMTIELGFA
                     WLTLPGDETIGIVDVPGHRDFIENMLAGVGGIDAALFVVAADEGVMPQTREHLAILDL
                     LQIPGGVIALTKTDLAPDPDWLDLVELDVHQAVSQTVLAKAPICRVSARTGAGLAELK
                     QALADCLRARPPRPDLRRPRLPIDRIFTMSGFGTVVTGTLTDGTLRLGDEVEVLPSGR
                     RGRIRTLQTHRQKQQSAQPGSRTAVNLTGLEVDEIQRGNVLALPGHYRTTQRVDLHFR
                     LLPDAAGPLTHSTEVKFFVGASEVVARARLLGSESLNPGESGWLQLELRDPVVTVRGD
                     RYILRRPSPSETLGGGVVVDAQPAKRHRRFDAAVLEHLEALLQGAPTDLLFRSILALG
                     ICTLSQAAAHARLSAEAAAAALDELYQSGEVIHLDGEGATALLAARPRWQALTDQAQA
                     ELAAFHRSYPLRAGTPREALKSRLKLEQRPFQAALRHWIAAALFVETGPLLALANFSV
                     QLNPAQQTQFKALLARFAAAPYTPPSIKECLAELGEDLYAAVVEQGLLLPVSAEVVFR
                     PADVAAMRQWVEEHLAAHGTLAATEFRDAFNTSRKYALALLEYLDALGVTVRDGDFRK
                     RRK"
     gene            complement(11185..12144)
                     /locus_tag="PKL11_02320"
     CDS             complement(11185..12144)
                     /locus_tag="PKL11_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12463.1"
                     /translation="MGNFYRYFIILCTLMLLTGCTPPTVLPPTSTTGAPTATQPEPET
                     PTRPAVETTLPASTETQTARPATATALPVFTPTPDLRQPPEDWQNWPIVPRVSARAIE
                     IYQTGLALGNNPLAFSKVGDCQSISEVLMGIYDQPMYYDRFDGEPDIQETIRQFAGSF
                     GRDGVAVNGGFNAAAVLSPIWADPDLCEAGETPIECEYRINRPSIVIISLEVWWEGRT
                     PEHYEQYMRQIIEFFIERGTLPILATKADNVEGDHSINLTTARLAYAYDIPLWNFWSA
                     AQPLPYHGMDPNRDDGFHIAPETWGTRSVTALRTLSAVWHAVK"
     gene            12358..12672
                     /gene="rplU"
                     /locus_tag="PKL11_02325"
     CDS             12358..12672
                     /gene="rplU"
                     /locus_tag="PKL11_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNZ12464.1"
                     /translation="MKYAIVENGGKQYRAVEGATIDVDLLPVEAGQQVDLSVLLVVDG
                     DQVAVGTPAVKGARVHATVAAHVKGPKVFTFKYSPKKRIRTKTGHRQQYTRLVIQSIE
                     ME"
     gene            12678..12959
                     /gene="rpmA"
                     /locus_tag="PKL11_02330"
     CDS             12678..12959
                     /gene="rpmA"
                     /locus_tag="PKL11_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNZ12465.1"
                     /translation="MAHKKGGGSVRNGRDSNAQRLGVKRYAGQYVLSGNILVRQRGTR
                     IKPGLNVGLGKDDTLFATAEGVVVYITLPTGRKQVSVMPVEVVVEQPAE"
     gene            13037..13240
                     /gene="rpmE"
                     /locus_tag="PKL11_02335"
                     /pseudo
     CDS             13037..13240
                     /gene="rpmE"
                     /locus_tag="PKL11_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            13229..13513
                     /locus_tag="PKL11_02340"
     CDS             13229..13513
                     /locus_tag="PKL11_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNZ12466.1"
                     /translation="MQARQSFVDQQRTREESRTSLARPVAELELGTRPTDALTKAGLT
                     TVGQILEKLQAGSEALLDVEGFGRKSLIDLKKKLRQLGYELPAAAEDTAV"
     gene            13654..14226
                     /locus_tag="PKL11_02345"
     CDS             13654..14226
                     /locus_tag="PKL11_02345"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNZ12467.1"
                     /translation="MKHHTGSIEVITGSMFCGKTDELIRRLRRATIARQKVQVFKPAI
                     DARYAVEKVASHAGAQFDAIPVKTAADILTLLEPDTTVVGIDETQFLGDEILGVAQTL
                     AGRGIRVIAAGLDMDFRGEPFGCMPALMAQAERVDKLQAICMVCGESASRTQRLVNGK
                     PARYDDPVIIVGASEMYEARCRQHHQVPRN"
     gene            14240..14803
                     /gene="hpt"
                     /locus_tag="PKL11_02350"
     CDS             14240..14803
                     /gene="hpt"
                     /locus_tag="PKL11_02350"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNZ12468.1"
                     /translation="MRDYREFLGEVLIPEDQLQARVAELGAEISRDFAGGEPLLLLCI
                     LRGGVVFLTDLMRHISIPHMIEFMAVSSYGVGHFQSTGSVRITLDLKTDIQNTHVILV
                     EDIIDSGHTIASVLELLSTRKPRSLSVCSLLDKASRREVFVPIRYTGFSIPNKFVFGY
                     GLDVDDYYRNLPFIGVVDLERYTEGGA"
     gene            14817..16313
                     /locus_tag="PKL11_02355"
     CDS             14817..16313
                     /locus_tag="PKL11_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ12469.1"
                     /translation="MNFDWGFPEVFSFLQAQPQPIYLVGGAVRNALLGLTSHDLDFAV
                     AGKAYPLARRLADHLKADVFQLDDVNDTARVLLTAPDGSRFRLDFATLRAASLEGDLR
                     ARDYTINAIALDVHDLSAMIDPLHGAQDLKDHVLRACSDASLQDDPLRVLRGVRLAVQ
                     HNLLVEPHTRQLLHQAAPLLCRVSVERWRDELFRILEGPRPAQAVRVLQQLGALTVIL
                     PELIELQGVQQTAPHVLDVWEHTLAAVDRLEQLWAVLVDGLPRPEADLMISLADMRLG
                     RFRAGLRQHFAAPLVPERSLRGLVFLAALYHDVDKPAVRSVDELGKVHFYRHEKTGAG
                     RAAERARALALSVAEVHRLSGIVHNHMRIHHMARDNRNPDRRAIYRYYQAADVAGVDI
                     CLFTLADTWATFGPTLTQERWLAEVDICRTLLEAWFEKPEEAVRPVRLIGGADLLTEF
                     NLQPGPLIGDLLYAVEDAQAAGEIATRPEALTYARNWIKLYGDLEEGV"
     gene            16317..17102
                     /locus_tag="PKL11_02360"
     CDS             16317..17102
                     /locus_tag="PKL11_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ12470.1"
                     /translation="MNSETVPLYYEEVGQGLPIVLLHGYPLNHTIWKPVADLLQDRAR
                     VIMPDLRGHGRSPVGEGEPSIRQMAEDVYALLEKLSIDRAIVVGQSMGGYVALSFAHA
                     YPRLLAGLGLIATQAEADTPEKRQARLQTASEVKRRSLKGVLAAMPPKMSPRADIQAA
                     THAIIADASRAGVIYALKAMADRPAALDWLTEIEVPAVVVSGLQDAFMPRERGITLAQ
                     MLGRAWLVELPDAGHMPMLEEPRAVADALLQVLDCVQTPGCQG"
     gene            complement(17093..17389)
                     /locus_tag="PKL11_02365"
     CDS             complement(17093..17389)
                     /locus_tag="PKL11_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12471.1"
                     /translation="MQTCSRCNTQTPDMALICPTCGAALRDYSNNAMALKRLQANPRV
                     TKIRVVVANDCCPVCQSNEGVYDKDKVPPLPTPGCSHHNGCRCTYEPILDQIYP"
     gene            complement(17408..17821)
                     /locus_tag="PKL11_02370"
     CDS             complement(17408..17821)
                     /locus_tag="PKL11_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ12472.1"
                     /translation="MSAGKPHIKRILVVDDDPAMTELLTLLLQPSGAFVITARDGLEG
                     LELVREFTPDVVLMDLMMPRMDGWKTCKAIRGFSSVPIIILSALDEPGMVATALDAGA
                     DDYLVKPVTSGELMAHIQRLVSRKPGQAVLSTAAA"
     gene            18060..20759
                     /gene="ppdK"
                     /locus_tag="PKL11_02375"
     CDS             18060..20759
                     /gene="ppdK"
                     /locus_tag="PKL11_02375"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ12473.1"
                     /translation="MSKKWVYLFDELDQAQAYVNNSLDALRGLLGGKGANLAEMTRIG
                     LPVPPFFTVTTEACNAYLAEGEKFPAGCWDQVITSLHKVEAASGKTFGDPKNPLLVSC
                     RSGAKFSMPGMMDTVLNIGLNDVTVEGMAKLTNNPRFAYDSYRRLIQMFGAVVLGIAD
                     EAFEDPLEAYKHSKGYKMDTELTADDLKELVKQFKVTVRQHMGFDFPQDPIEQLRLAT
                     EAVFKSWNGKRAIDYRRAEGIPDNLGTAVNIVTMVFGNMGMDSGTGVAFTRNPATGER
                     KLYGDYLLNAQGEDVVAGIRNTDPVQKMGETLPEAYEEFLKICAILEKHYKDMQDMEF
                     TIERGRLWMLQTRNGKRTASAAVRVAVEMVAEGLITKEEALLRVTPKQVDALLHPQFN
                     DTAKKAAKKEGRFFASGVNASPGAAVGQIYFDADTTERMAKESGQAVIMVRPFTKPDD
                     VHGMLAAKGILTSEGGATSHAAVVARQFGVPCIVGASAVKIDLERRVMRVGDVEVKEG
                     EWISVDGGTGEVYLGKLELTVPNLEEQKELMTLLQWADEASRLQVWANADYPKDARRA
                     RSYGAKGIGLCRTEHMFFETVRLPIVQRMILAETSAERTAALDELLPYQRADFDGLFE
                     AMDGYPVIIRLIDPPLHEFMPDEEKLLEEVIAMRIKGETKGLPEKEKLLASIRGMHES
                     NPMMGLRGVRLSIYMPEIVEMQVRAIFEAAADCTKRGIVVKPEVMIPLTGTVAELKWI
                     QPRLVRIAKAVTEEKGVSFHYKFGTMIEIPRAAVTAAEIAGLAEFFSFGTNDLTQMTF
                     GYSRDDAERNFLIKYVEEGILPKNPFQTLDRDGVGRLMRMAVEDGRRARPDLEVGICG
                     EHGGDPDSIEFCHLIGNNYVSCSPFRVPVARLAAAQAAIKHGK"
     gene            20948..21907
                     /gene="pdhA"
                     /locus_tag="PKL11_02380"
     CDS             20948..21907
                     /gene="pdhA"
                     /locus_tag="PKL11_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNZ12474.1"
                     /translation="MDKAEHLKLYKEMVVIRRVEERAAELYQQGKIGGFLHLYIGQEA
                     VSTGLVSVRQPVDRVITAYRDHGVAINCGIPARQVVAELLGKATGCSGGKGGSMHMAD
                     VEKNFWGGHAIVGAHLPLASGLALGDVYAGNKAVTLCMFGDGATNIGYFHEAVNLAKV
                     WNLPVLWVCENNQYGMGTAVDRASAVSEIIAKACGYGMRAEQVDGMDIMAVRRVAGEM
                     IAEIRAGSGPQLLEVLTYRFRGHSMGDPERYRQASEVHQWQENDPIGIYRKYLVDNNI
                     ATTAELDEQDDLAEAEVTDAVAFAESSPEPAPEELFKHIYVEE"
     gene            21917..22888
                     /locus_tag="PKL11_02385"
     CDS             21917..22888
                     /locus_tag="PKL11_02385"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNZ12475.1"
                     /translation="MAKITMREAISQALWEEMERDPRVFILGEEVGVWGGTYAVTKGF
                     YDHFGAERVRDTPIAEAAIVGAAIGAALTGQRPVAELMTINFAFSAMDHIVNEAAKMH
                     YMFNGQMRLPLVIRTVGGGGRQLGATHSQTPDAMFAHFPGLKVVAPGTPADAKGLLKA
                     AIRSEDPIFFIEHATLYQTRGEVPEGDFVVPIGKSTIQRPGRDVTIVTYSKMLELSLK
                     AAEQLAAEGIEAEIVDLRSLRPLDMDPVLESVRRTHRAVMVEEGWKSFGVGSEISARI
                     HEEAFDDLDAPVKRVAQLEVPLPYNRALEQMALPKVEHIIQAVKEVL"
     gene            22888..24114
                     /locus_tag="PKL11_02390"
     CDS             22888..24114
                     /locus_tag="PKL11_02390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNZ12476.1"
                     /translation="MADTVSMPKLGFDMAEGTLVRWVKAEGEAINKGDVLAEIETDKA
                     TVEVESGFGGVVARHLVAQGSIVPVGAPIAIIAAPGETVTDAAPAAAPAEKPAAAPAA
                     APTPAPAPAAEIPPAVGGRVKASPLARRMAAERGLALEGLRGTGPGGRIVKRDVEAAA
                     QTPPPAPVIPTAAPLSVTQPHAAEQVPVDRLRAAIGRRLVESKQQVPHFYITHQCDAG
                     PLMRLRKEVNEMLPEDGRLSVNDFIVKAAALALRQFPNLNASLQGDVILRHGAVNIGV
                     AVAVPGGLLTVVSKDADQKPLRVISAEVREMAARARAGKVRPEDIEGSTFSISNLGMF
                     DVESFVAIINPPEAAILAVGSVKEIPVVVDGVVQPGLRLNVTLSADHRITDGAEAAQF
                     LQALAEFIEKPARMLV"
     gene            complement(24221..26614)
                     /locus_tag="PKL11_02395"
     CDS             complement(24221..26614)
                     /locus_tag="PKL11_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNZ12477.1"
                     /translation="MKKIIIFLLFTLLLTACNLPFNQAEPTLVPTATTAPTATPQPTP
                     TPTPTVPPTVRVEQGDALLLTGDYPAALQEFQAALNAAPDTETQAAALLGVGQTYAIN
                     GDCASALSVLQNLLTQYPQAIAAAPAYYQIAECHMQTGLPALAAQEYLGYIQLRPGVL
                     DAMMYEKRGDALQAAGDYLAAVEAYDAAIAASSGITDDLQIKKGSAYAAVSDYTNAIR
                     TFTAVYESTASDYTKATANLLAGQTYMALGLPEQAYARYQESVLFFPTSYDTYTGLVA
                     LVNADQPVDEFNRGLVDYYAGQYGYAIEAFDRYLKANPTHDGAVHHYKALCQRALDQP
                     EQAIAEWQALIQDHSGDRFWADAHDEIADTYWYWLGDYTRGAQILLDYIEKVPGSDYA
                     PGFLFRAAQIMERGGYLSQAAEAWERLMNEYPNAEDSYYGLFLAGITYYRLGNYPQAL
                     LVFQRVLVLAGEPGDQAMAYLWIGKCQLAQNDAAAAQASWKEAAQRDPTGYYSERAAQ
                     LLAGQAPFTQPAVYDLAYDLTSERVEADTWMRVTFGLPAETDLSGLDALAADPRMVKG
                     NALWELGLYRSARGEYETLRLNVLNDATATYRLMNFFLERGLYRSAILSARQVLDLAG
                     LDDAGTLEAPAYFNHIRFGAYYKDLVLDAAAQEGVPPLLVFAVIRQESFFEGFAESSA
                     GARGLMQIMPATGQEIATSMSWPVGYTTDDLYRPAVNIRMGTRYLSRQYIYLNSDYYG
                     ALAAYNGGPGNARSWSELSGTDPDLYLECIRYDQTRDYVRRVFENFEIYASLYAREP"
     gene            26809..27870
                     /gene="mtnA"
                     /locus_tag="PKL11_02400"
     CDS             26809..27870
                     /gene="mtnA"
                     /locus_tag="PKL11_02400"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNZ12478.1"
                     /translation="MLTLWWDDTTETVQMIDQRALPARLEILSCPDAAAVAEAIRNMT
                     VRGAPAIGVAAAYGLALAAWHSPATDPADLRTDIMQAAAMLRATRPTAVNLAWALDQM
                     LRIADTPGLTVDKLRYRLLAEARRLAEEDIATNRRMAEFGAALIDDGDTIIHHCNTGS
                     LAVVDYGTALGAIRRAHEQGKRIFVLVDETRPRLQGARLTAWELTQYGIPYAIIPDGA
                     SGHFLRRGEANKVFFGADRVAANGDVANKIGTYMLSLAAHANDVPVYAVMPASTIDLS
                     LPDGSHIPIEERSADEVLNLTIHGEPIAPAGAAARNPAFDVTPHTLITALVTDRGVVY
                     PPFHIHLPRLLNGTQSGSL"
     gene            27881..28840
                     /locus_tag="PKL11_02405"
     CDS             27881..28840
                     /locus_tag="PKL11_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNZ12479.1"
                     /translation="MTIVCTGSIAYDYLMTFPGYFKDHILQERLDCISLSFLVDSMVR
                     QRGGTAPNIAYTLALLGDRPLVFGTVGEDFEDYRTWLESKHVDTTFARLIPGVFTASF
                     FCNTDRSNAQIASFYPGAMGFASQLSLAELTQYKVDMVVISPNDPQAMDRCVRECQQM
                     GLPYLYDPSQQVVRVSGEELRRGVQGAYSLFCNEYEFELLQKHTGFSADEMRSLVKIL
                     VVTLGDKGASIFTAGQEITVPVVPAREIVDPTGVGDAFRGGFLRGWQLGLDWATCGQM
                     GALAATYVLEQRGPQSHSYTLPEFVARYRQHFDDHGALDVLTR"
     gene            28963..30222
                     /gene="ahcY"
                     /locus_tag="PKL11_02410"
     CDS             28963..30222
                     /gene="ahcY"
                     /locus_tag="PKL11_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNZ12480.1"
                     /translation="MTQYDVKDLSLAEGGRRRIEWAAREMPVLRSISERFAKERPLAG
                     IRMSACLHVTAETANLMRTLQLGGAEVVLTASNPLSTQDDVAAALVSFSEIPTYAVKG
                     EDNVTYYRHINAALDHRPHITMDDGADLVGTIHKDRRDLLSEILGGTEETTTGVIRLR
                     AMAADKALMFPVIAVNDAMTKHFFDNRYGTGQSTIDGIVRATNVLLAGKVFVVAGYGW
                     CGRGLAMRARGLGANVIVTEIDPLKALEAVMEGYRVMPMIDAARIGDIFCTVTGDLNV
                     LDRHHFEVMKDGAIISNSGHFNVEINIPALAEMSVEKKLVRPFVEQYVTKDGRSLFLL
                     GEGRLINLASAEGHPASVMDMSFANQALAAEYMVRNGKSLERRVYSVPEEIDHEIARI
                     KLSAMGIQIDTLTPEQVAYLGSWQEGT"
     gene            30330..31004
                     /locus_tag="PKL11_02415"
     CDS             30330..31004
                     /locus_tag="PKL11_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12481.1"
                     /translation="MKRFFALLAVLFLVTLALAACTTPTPAPTFTPAPTDTATPQPSA
                     TLLPTLTDTPTPLPTATLTATATWTPTPTPDFSQVRITAQDYRAATSNYLIILAFPGI
                     KEPYLTRLNGNSYNCVLDENYPDQYFCVGPITGYMKPVAAEFTRPGETTPFFTGYIGG
                     YPFPEPTPLPVGAPDTWCPLRGQHITCEPEYYTKYDCWITTCYDLCGYYYSVDTCLAV
                     TPTVGP"
     gene            31025..31699
                     /locus_tag="PKL11_02420"
     CDS             31025..31699
                     /locus_tag="PKL11_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12482.1"
                     /translation="MRKLTWMGLLILVAMILAACTPAPTAVPTALPTATNTETLAPTE
                     TPVPTDTPVPTATNTPTLTPTITLTPTATPDYSVIQVIWQESYTNRQPIVALKIPGIT
                     EAYGAKINNVAYTCSIDANTPDTLFCNGPLLPYFTYVQLALYHPGDALPFYEATIPGP
                     VRALPTATPVGNPNTWCPDRGQNVHCETENRTYPGGLQCVVSTCYDACGYYYSVDTCP
                     TTTPNP"
     gene            complement(31776..32348)
                     /locus_tag="PKL11_02425"
     CDS             complement(31776..32348)
                     /locus_tag="PKL11_02425"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNZ12483.1"
                     /translation="MRALKRFSWLGMMAVVLLLGLTACSLPQPERQAPQADAPLFVPP
                     TAIPPTPTTESVQAVPQAATPGGPCEDLLSFIADVTIPDGSQVQPGSTLDKRWEVQNS
                     GTCNWNENYRLRLIAGEELGASSEQALTPTRSGTNTVIRIVFTAPLEPGDYRSAWQAH
                     NPDGLPFGDPFFIDFTVIQPEAGVTPTLSP"
     gene            complement(32359..33453)
                     /gene="recA"
                     /locus_tag="PKL11_02430"
     CDS             complement(32359..33453)
                     /gene="recA"
                     /locus_tag="PKL11_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNZ12484.1"
                     /translation="MARKSSPPAESSAQNSSMDPGRRAVLDRALGDIVKRYGEGSIMR
                     MGEAHQLAVEVIPTGSLSLDIALGVGGLPRGRITEIYGPESSGKTTLCLHTVAEAQKM
                     GGTAAYIDMEHALDPAYSTRLGVDIDNLLIAQPDMGEQALEIVETLVRSGAVDIIVID
                     SVAALVPRAEIEGDMGDASMGMQARLMSQALRKLSGAINQTKTAVIFTNQLRQKIGVM
                     FGNPETTSGGMALKFYASVRLDVRRIQSIKVGADIIGNRVRVKVVKNKVAPPFRVAEF
                     DIMYNEGISKVGDIIDLGTQLEIVTKRGAFFSYGEQRLGQGRENAKEFLRQNPALSNE
                     IETAIRQQVLSGEAVITLGGGDGEAEDDGE"
     gene            complement(33554..34477)
                     /locus_tag="PKL11_02435"
     CDS             complement(33554..34477)
                     /locus_tag="PKL11_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12485.1"
                     /translation="MKCKKCGERAVIKMPQHHLALCRQDYIDWFIAQTARSIKKYQLA
                     RPGDRILVAVSGGKDSLSLWDVLWRLGYAADGMYIHLGITGDGDYSTQSQRYAEDFAA
                     VRGLSLQVVNVTERYGQSIPDLAGQSARGRYKPCSICGQTKRHIMNEAAIRGRYDVLM
                     TAHNLDDEAAVLYLNTMNWAVPMLARQAPRLEAHDGLPLKAKPFCRFYERETAAYALL
                     QGIEYIEEECPFAEGSTLLYYKTVLNRMEAERPGAKMQFYVQFLQARTALFPGPVQEA
                     DRAGLHPCPNCGQPTTSAGVCGFCKLMGSKT"
     gene            complement(34474..34674)
                     /locus_tag="PKL11_02440"
     CDS             complement(34474..34674)
                     /locus_tag="PKL11_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNZ12486.1"
                     /translation="MAVTIILRKQEHILPHAPTVKRAMELVGVTPETYLAVRDGELLT
                     EDERLNDGDVIKLIPVISGGSR"
     gene            complement(34689..35057)
                     /locus_tag="PKL11_02445"
     CDS             complement(34689..35057)
                     /locus_tag="PKL11_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12487.1"
                     /translation="MAERVRFEIFQDIQLLVEDFSETSHEELLQTAASARKMIQSQPP
                     RSLRILVLAYRPHIDVDTVRALKDTATENAPFAMATAVVGLDSFMKMLLKGVEVVSGR
                     NFTPFDSEELARKWLAEQKP"
     gene            complement(35079..35558)
                     /locus_tag="PKL11_02450"
     CDS             complement(35079..35558)
                     /locus_tag="PKL11_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNZ12488.1"
                     /translation="MAEFSRYEVYMLLNQPKPLWLSHLNLSGMDLRGVDLRGATLIKA
                     DMTGTHLEGVDLREANLGGATLQGSIIVGADLRKANLRGANLRNCDLREANLAEADLY
                     EADLAGANLLDANLGGADLTNANLSGANLLRANVPDYRILRAKSLTGATLPDGTRSD"
BASE COUNT         6979 a        11007 c         9893 g         7679 t
ORIGIN      
        1 tccctgccga aacagtcaat caactgggct tgctgggctt ctctggcatt gccaacgttc
       61 tctcggccat caagtttgcc aaatactacg aactgggcga gaccgatgtg gtcctcactg
      121 tgctgaccga ctcaatggag ctttaccaca gccgaattga agaaatgcac gccgaatttg
      181 gcgcctacac cgagctgaac gccgccgccg attttgcccg ccacctgctg ggcgtgacca
      241 ccgacaacct gctggaactc tcctttgttg agcgccgccg ggtgcacgac ctcaagtact
      301 acacctgggt ggagcagcaa ggcaaaacct atgaggaaat tctggcgcag tggtatgatc
      361 ccacctactg gaccgacctg catggtcagg cggccgaaat tgacgccctg attactgaat
      421 tcaacgagcg cgttgggctg atccaaaagt accgctaacc gccatggatg cctggcctcc
      481 atccaccgca ttgatcctgg tcggggtgat gccctcaccg cgtgacctgg agattgcgcg
      541 ggtgctgggc tggtatcgca ttcccctgcg caccgccccc aaggtagtgg cagtggatta
      601 tctggcgttt tatcaaaccg cagcgtttgg cgaagaccac ggcgcgcgga ttgagtgtgt
      661 ggcccctgtg cgcggccacg aactgaccac ccgcgccgag ctgctgcgcg ctgagcccga
      721 ccatccccgc gcccacgagg aatattacaa actcttgctt gggccgctgc aaaccctgcc
      781 gcggcccatc acctcgggcg cctggcggcg gctcaccttc ctctacacca ccggcgaact
      841 gctccaatcg gcgcaaaccc tggatgacct ggtggtgcgc tccgatgaac gcgccctgct
      901 ctgggcctcc ctgcgcgaac gcgccgccca ggcgggccaa tatcacgccg ccgacctgcc
      961 cgaactgccc ctcgatcccg ccatcctgct catgctgggc gggttgggag atatggatcc
     1021 tggtaattaa cgttgtgagg ttatagataa agatgtcaaa taaaaataac tctggtaaga
     1081 aaaccagaga accgcaattg gcttataacg ttatgacgct tgcgccaatt gtgaaggcgc
     1141 gcccttttgt taaatgggct ggtggaaaat cgcaactatt accagcatac gttacctatc
     1201 ttccatcgtt atcccaaata caaagatatt ttgagccatt tttaggggga gccgcagtct
     1261 ttttctattt gcaacctgaa caagcatttc tttctgatag taatgctgaa ctcattaatt
     1321 tatactcagt tgttcaagat agggttgaag aattaattgt tgaattaacg gcttatcaaa
     1381 acgacgaaga tttttactac gcagttagat cgaaggatcc tgggtcactt accccaattc
     1441 agaatgcagc cagatttatc tatctgaata aaacatgcta taacggattg tatcgtgtga
     1501 attctagtgg acagtttaat gttccttttg gaagatacgt taatcctacg atctgcgacg
     1561 cagaggtgtt acgatctgct agccttgcat taagtggagt tagtttgact gttgcggact
     1621 ttgaaaaagc tgtatcctct gcttctgcta gagactttat ttactttgac ccaccctatc
     1681 atccggtaac aaaaacgtct agctttactt catacacaat gggaaaattt ggagatgatg
     1741 aacagcatcg attagctaga atttttaaag aattagatca aaaggggtgt ttggtgatgt
     1801 taagcaattc tgatacgccc ttaattcgag agctatataa ggcttataaa attattgaaa
     1861 ttcaagcaaa tcgggcaata aattccaatc ctgaaaaacg tggaaagatc actgagcttt
     1921 tgataatcaa ttatgggagg tgaatcgtgg ttagtaaaaa tgattatgct tggaaaacgt
     1981 atattagcaa aaataatttg tcattagacg gccagccata tcgtgtaata gccaaacaat
     2041 tacgtgagca aacaggcaga gaaccccgct taatggcaaa gtttgacgaa ccagctcaag
     2101 tatctaaacc attatcagaa caaaattaca cattattggc cgtaacaaat ttggaatatg
     2161 ttttgtttca aggtacgact tttactgaag taccagcatg tacagatgca aggaaggtta
     2221 gtgcttcatt gccattttct cttgtaaccg cgggacgcgg aactggagaa tcagaatatt
     2281 tggacaacgc atttaattca gggatattac aagattttag cggttgcgat cctcttttct
     2341 tgactattcg gggcaaggaa cgttcaagga attttgattt ttatattaat aatgctcaat
     2401 tgagtttaca aactgatggc gttcagattg aagttgatgg cggatatgaa ggggaaaggg
     2461 atgtaatttt agttgaagcc aaaatcggta accgaaaaca tttcaatatt cgacaacttt
     2521 attacccata tcgtcatttt tccctcatag ctccccaaaa aaggataagg cctatcttat
     2581 ttttgtatga tttactgtct gcttcatata ctttttatga atataaattt tcatcacaga
     2641 atatatttga ttcgattgaa cttgtaagat gttgtcgcta taacctaacg ccgggtaaag
     2701 gattgagatt gtctgatttg atagatgtag gttttgaaac gacaagtgac cttattccac
     2761 aagcagatga ttttaacaag gttttagaat tattgacagt aatcaatagc ggcttagata
     2821 cagctcaaga tattgcagat tattttggtt ttgagcctag acaaagttac tattatgctg
     2881 aagctgcaaa atttttgggc ctgatttgtg acgagggatt tcaattaacg gagcgcggca
     2941 ctcaattttt aagaacccca ccaaaagatc aacaactatt agcagcaaaa ttgcttatta
     3001 atagtgagct ttttaggaga ttgatatctg ttgcaagaag aaaaggctat tatgctaaag
     3061 acgatgttgt acaagcgatt ttagatgtag gtaaatataa cttgacaact tctaacagaa
     3121 gaaaacagac tgttatgtca tggagtaatt ggttggcgaa tcagattggt tgttttcgtg
     3181 aacaagaagg aaagtacttt attaattgat attttcttgg taaattgtct ttaagtaagg
     3241 agatatttca tatgcgcaaa ttacttatcc gcaatggcac cctggtcacc gcggccgaac
     3301 tctacccggc cgatattctg gtgattggcg agcagattgc cgccattggg caaaacctgc
     3361 ccgccgacga cgccgaggtg gtggatgccg ccggcaagct gatcctcccc ggcggggtgg
     3421 atgtgcacac ccacttcgat ttgcccatgt tcggcaccgt ctcatctgat gatcactaca
     3481 ccgggcacaa ggccgccgct tttggcggca ccaccaccgt gattgatttc gtctcacagg
     3541 atttcccctc gctttcagac agcatcgccg cctggcacgc caaagccgac cccaaagccg
     3601 ccgtggactt tggcttccac atgaacatta cccacctgga cgcggcgatt gaggccgaaa
     3661 tggcagacct gcccgggctg ggcatcacca gcgtcaaggt cttcaccgcc tacaataacc
     3721 gcctgcgcct gtctgatggc gatatctttc gtgtgctgcg gttgggcgcc cagcacggcc
     3781 ttttgacttt gatgcacgcc gaaaatggcg atgtgattga tctgctgact gccgaagcgc
     3841 tggctgccgg ttacaccgcc cccgaatggc atgcctacac ccgccccagt tggggcgccg
     3901 tagaagccag cctgcgcggc gccgcgtttg ccgcccaggc cgatgccccg ctttacatcg
     3961 ttcacatgaa caccggcggc gaggtggata tgctccaata tgcccgccaa aagggcctgc
     4021 ccgtgatggg cgagacctgc ccgcagtacc tcttcttcac cgtcaatgat ttgcgccggc
     4081 ccgatggcgc caaatgggtt tgctcgccgc ccatgcgcac tgcaatggac aatacccgcc
     4141 tctggcaggg tctgatggat ggcaccatcc aggtcattgc cactgaccac tgccccttct
     4201 tttttgatgg cactcagccc atcacttacg aggggcagcc catcgccatc cccggcaagg
     4261 aacttggcca gagcgacttt accaaaatcc ccaatggcct gcccggtgtg ggcgaccgcc
     4321 tgcccgtcct ctggacgcat ggcgtcttgc gctcccgcct gacccccagc cagtttgtgg
     4381 cgctcaactg caccaacccc gcccgcatct ttggcctcta cccccgcaag ggctccctgc
     4441 tccccggcgc cgatgccgat atcgtcatct gggaccccgc tctgaccacc ctctatggcg
     4501 tggcacaggc ccagcaccgc accgattaca acctctatga gggctgggaa ctgaccggca
     4561 tgcccgccca ggtctatctg cgcggccaac tgattgtgga tggcagccgc tggcttggcg
     4621 cagccgggcg cgggcacttt gtaccccgcg gcgcccccga aatcatttaa cccgggtaaa
     4681 atttacacat gcttgtcctg ctgccggcat tgttgctgat tgtcctgggg tttgccctgc
     4741 tggtcatcca gcggatgcgc cccaacttcg gctatgcctg gctgggcgcc tcggcttcaa
     4801 ccctcatcgt ctgggggctg atgctttacc tgcgctttcg gctgccctca gaactggtga
     4861 ttgaaaactg gcgcgccttt tcagccctgg gcagcgattc actggtcttc caggtggatt
     4921 acatctcctg gccttatgcc ctggcgctca gcactctgat tgccgcagtg gtcatcagct
     4981 cgcctgctcg cctggatgca ggcggttccc tgccctcctg ggaccgcggt ctgatgctgg
     5041 tgggcatggg cctgctggcg gtttttgccg ccagcccgct ggccctggtt ttgatgtgga
     5101 ccgccattga tgttgtagaa ctgctggcgc tgctgggtac cgcctccggc gaccgcctgg
     5161 agcgctctgc cgtgacttct ttcgcattcc gggtcaccgg cacgctgctg gtgctgctgg
     5221 ctgtgctgca gggcggtccc ggcgcaacct ttgattttat ctcccacagc cagcatttga
     5281 gcctgctgct tgtcctcggc gctgggctgc gcctgggcgt tttaccgctc aatgttccct
     5341 atgccagcga acccgtttta cgccgtggca ttggcaccct gctgcgcatg gttgccgcgg
     5401 cttccagcct ggtgctgctg gcccgcctgc ccgaaaatgc cgttcctgcg gcctgggtgc
     5461 cctggctgct gatcctggca gttctggcgg cgctctacgg cgccgggcgg tgggccgccg
     5521 ccaaagacgc cctcagtggg cgcccttact ggctggtggc catggctgcg ctggcgattg
     5581 gctgtgtggc tcaagggcag ccctcggcca gcctggcgtg gggtgtggct atgattttgc
     5641 ccggctcggt gcttttcctc ttctcggcgc gctctaccgg gctgctggcg ctgcccgctc
     5701 tgggtctgat tggcctgctg ggcctgccct tcaccccggc tgcgggcggc tgggtgggat
     5761 tgctgggcag tcccatcaat ggcaacgggc tgctcttcct ggttgcccag gcgctgctcg
     5821 ccattggcta catccgccat gctctggctc ccgcgcggcc ctggtccgaa ctggaaacct
     5881 gggcccgggc ggtttacccg ctgggcctgg cgctgttgat tgtggccgat attctgattg
     5941 gcatctgggg cggtccgggt tttcgcaccc tgggcatctg gtgggcagcg gttatttcca
     6001 ccgccttcat cggatttttg gtcattggcg gccgtttgct gcggcctgcg gcgcgcgttg
     6061 gctggctggt caccctgggg cagcgggtgg tcaattttct atccaaaatc atcagcctca
     6121 cctggtttta ccgcctgctg gaatggttct acgcccggct gggacaggtg gtacgttttt
     6181 tgagcctgat ctttgaaggg gatggcggtg tgctctggac cctcgtgttc ctggccctgc
     6241 tgattacact ggtccgcagt caggtcacgc cataatggaa acactgcttt cggttattgc
     6301 gctaatttta ctgctgggcg cctcctgggt gctgctgctc agccatcact ggcgctggag
     6361 catcgtggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ccgcccgctg
     6481 cggattattc tgggcttgct gaccaccctg gccgggtttg aactgctgta tgccgcagtt
     6541 gaagattcga ttatggttgc cgggctgctg gctgcgctca atctcggcct ggccctggcg
     6601 ggtgcttact tcctatccct cagcacgcca gaggagtccg catgagcgcc ccgctgatct
     6661 ggatcgtcat cccgttggtt ctgaccggac tgctctggct gctgcaccgg cagccaaagc
     6721 gcgccgccct tgcaatgctg atcatctgcc tcgtgctggt gattctggcg gcagccttac
     6781 cgattggctc cttcattaaa ataggtcgta ctacctttga attgcccacc acactcaccc
     6841 tgtttggccg ccgtctcgtt ttagaggcct cggaccgcat ctggctgatg ctggtttatg
     6901 gcctgggcgc ggcctggtcg ctgggcattg tgcagtctcg cgtgcaccgc tcttttaccc
     6961 ccctttcact gttgatgatt gtgctgctgg tcgccgcgcg ggcggtggag ccgtttcttt
     7021 atgccgccct gctggtagcc gtggcggtgc ttgccagcct gccgttgatg ctgccgccgg
     7081 atgcccgtcc ggggcgcggc atcatgcgct acttgatctt tcagacgctg gctgtaccct
     7141 tcattttgct ggcgggctgg gcagcatccg tggtagatgc caacccctcc aatgaggttt
     7201 tgcgactggt tgccagcgcc tcgctggggt tggggctggc cttctggttg gcggtcttcc
     7261 cgttttattc ctgggtgccc ctgctcgccg gtgaagccca cccctttgcg gctggttttg
     7321 tgctcagcct gctgcccacc actgcactct tccttggcct cgattttttg aacggctttg
     7381 gctggctgcg caattctgaa attatcctca ccgccctgcg ccttgttggc gtgttgatgg
     7441 tagtcacggg cggcatctgg gctgctttcc aggaagacct gacccgtctg ttcggctatg
     7501 ccatcatcgt ggatctgggc gcggcgctgc tttccatcag tctgcgcagt gatattggcc
     7561 tgactatttt cgccgccgga ctgatccccc gtctgctctc gctgttcatc tgggcgctgg
     7621 cgctggcttt cctgcgtgtc cgtcagattc cgctgacctt cgccggagtg actggtttgg
     7681 ggcgccgtct gccgattttg tctgctgcgt tggtgctggc ttgcctctcc ctgggcgggc
     7741 tgccgttgct ggcggtcttc ccggcgcgtc aggccctgct ggtggaactg gcatctcaat
     7801 cgtccattgc ggcggcctgg gcgctggctg gcagctttgg cctgctgctg ggtggcttcc
     7861 ggctgctgaa tgtactgctc aaacgcactg aacccggctg gcaggtaacc gaaaacctgt
     7921 ccgaaggctt gctgctctct gctggtataa tcattcttct actgattggt attttccccg
     7981 gcggcatcct gggcagcctg cccgataccc tgccgctggc tttccccaac ctgccctgag
     8041 gattgttttt tctggaggaa cgcatggata tagagcaatt tgttgcccgt ctggaatggc
     8101 tggaagatga gcgccgtaaa gataaactga ccatttccac cctgcaggag cgcctggctt
     8161 cccttgagtc atcacttccc ggcatggtgc agcaaattaa agacctgacg ggtgatctgg
     8221 cccgcctgac ggcctctctg gcccgcattg accaggtgga aagcgcaatt gcccaggtgc
     8281 gcgtcgaatt ttcccgcgcc ctcgatactt ctgaacgcac tcgcgccgag catgatcgcg
     8341 aagtggaccg tgcgcgccag gcggatatgg atgtggtcaa taaatcgttt gccgaactgc
     8401 gcaaaagcat cgaatcaatt ggcgaactgc gccgtgggct gcagtcccgc accgaagaag
     8461 acttccgcct ctcccgcctg attgaagagc tggagcagaa ggtcacccag acccgtcgct
     8521 ctgacgatga atatcgccat gcccagaaat tgattgagga tgggcagcgt caagaggcca
     8581 aacgagtcac cgacctgcag ggcgaagtag ccgccattcg caaacgcatg gaagaacagc
     8641 gcggcaagct ggatgtgacg gtggataccc tgcgcaaggt ggaaacccgt ctggcagact
     8701 tccagaccac agagggtgaa cgccgccagg cccaggtggc cttcatcgaa aaacaaaata
     8761 tgttgaacgt ggatcgtgat cgcacctgga aggaatggca gacccgtttt gagcaaatcg
     8821 actccgtctc caccaatctg gatacgcagt tgcagtctct cgatgccacc caccgcgcca
     8881 tcaagcgtgc ccaggaaggc tttgaagaaa tcacgcagcg ctttgagcgc cgcatcaacg
     8941 aaattaccga gatgcagcgc ctcagtgaag accgtttccg ccaggagtgg gtggggttcc
     9001 gtgcagacga tcaaaaacgt tggactaact acaccctctc ccaggaagag cagcagcgtg
     9061 aggccgggcg ccagtacgac aagctggttg agcgcatgac cctgattgaa gaatcaaccc
     9121 acgaattgaa cgacgccctg acccagaacg ccgaacagac cctcaaacgc ctgcaggggt
     9181 tgatggccct gtctcgtgag tggctggaaa tgcaggaaca aaacggtaac tcgctgcgga
     9241 gctaggcacc agccatgcat gtgattggca ctgccggaca tgtagatcat ggcaaatcaa
     9301 cgctgattgc cgcgctgacc ggcacccacc ctgaccgtct caaagaagaa cgcgaacgcg
     9361 agatgaccat tgagctgggt tttgcctggc tcaccctgcc tggcgatgag acaataggca
     9421 tcgtggacgt tcccggccac cgtgatttta tcgaaaacat gctcgccggg gttggcggca
     9481 ttgatgccgc tctatttgtc gttgccgccg atgagggcgt gatgccccag acccgcgagc
     9541 atctggcaat tctcgacctg ctgcaaatcc ccggcggcgt catcgccctg accaagactg
     9601 acctggcgcc tgatcctgac tggctggatc tggtggaact ggatgtgcat caggcggtca
     9661 gccagaccgt tctggcaaag gcccccattt gccgggtttc ggcgcgcacc ggcgccggtc
     9721 tggcagagct gaagcaggcg ctggcagact gtctgcgtgc ccgccccccg cgccccgacc
     9781 tgcggcgccc gcgcctgccc attgaccgta tttttaccat gtccggcttt ggcaccgtgg
     9841 tcactggcac cctcaccgat ggcactctgc gcctggggga tgaggtggag gtgctgccat
     9901 ccggacggcg cggccgcatc cgcaccctgc aaacccaccg ccagaagcag caatctgccc
     9961 agccgggtag ccgcacagcg gtcaatctca ccggccttga ggtggatgaa attcagcgtg
    10021 gcaatgtgct ggcgctgcca ggacactacc gcaccaccca gcgggtggac ctgcattttc
    10081 gcctcctgcc cgatgccgcc ggcccgttga cccactccac cgaagtgaaa ttttttgtcg
    10141 gcgcttccga ggtggtggct cgggcgcgcc tgctggggag cgaatccctc aatcccggcg
    10201 aatcgggctg gctgcagttg gaactgcgtg accctgtagt gacggtgcgc ggtgatcgtt
    10261 acattctgcg ccgtccgtcc ccttccgaaa ccctgggcgg cggtgtggtg gtagatgctc
    10321 aaccggccaa acgtcaccgt cgttttgacg ctgccgtgct ggagcacctt gaagcgctgc
    10381 tgcagggtgc cccaacggac ctgctcttcc gttcgatttt ggccttaggt atttgcaccc
    10441 tgtcacaggc tgccgcccat gcccgtctgt cggcagaagc tgccgctgca gccctggatg
    10501 aactgtacca gtccggcgaa gttatccatc tggatgggga aggcgccaca gcactgctgg
    10561 cggcgcgccc gcgctggcag gcgctcaccg atcaggcgca ggctgaactg gccgcgtttc
    10621 accgcagcta cccgctgcgt gctggcaccc cgcgtgaggc ccttaaaagc cgtctcaagc
    10681 tggagcagcg ccccttccag gccgccctgc gtcactggat tgccgccgct ctgttcgtag
    10741 aaaccggccc gctgctggcc ttagccaatt ttagcgttca gctaaacccc gcacagcaga
    10801 cccagttcaa ggctttgctg gcgcgctttg ccgctgcacc gtacaccccc ccttcaatca
    10861 aagagtgcct tgctgaactg ggcgaagacc tttacgccgc ggtggttgag caagggctgc
    10921 tgctgccggt ctccgccgaa gtcgttttcc gcccggcaga tgttgccgcc atgcgccagt
    10981 gggttgaaga gcatctggcg gcgcatggca cactggctgc taccgaattt cgcgatgcct
    11041 tcaacaccag ccgcaaatat gccctggccc tgctggaata tctggatgcg ctgggcgtca
    11101 ccgtgcggga tggcgacttt cgcaagcgcc gcaagtaagc attttccctg aaccctgctc
    11161 agcaagctga ccggatcggt ggtcttactt gactgcgtgc cagaccgcgc tcagtgtgcg
    11221 cagagccgtc acgctgcggg tgccccaggt ttcaggcgca atatgaaacc catcgtcccg
    11281 gttggggtcc atcccgtggt acggcagcgg ctgggccgct gaccagaaat tccatagcgg
    11341 gatgtcataa gcataagcca gccgggctgt ggtcaggttg atgctgtgat ccccctctac
    11401 gttatccgct ttggttgcca gaatgggcag ggtgccgcgt tcaataaaga actcaataat
    11461 ctggcgcata tactgctcgt aatgctcggg cgtccgcccc tcccaccaca cctccaggct
    11521 gatgatgaca atactgggtc ggttgatgcg atattcacac tcaattgggg tttcgccggc
    11581 ttcgcacagg tctgggtctg cccagatggg cgagagcacc gccgccgcgt tgaagccgcc
    11641 attcaccgcc accccatcac ggccaaaaga cccggcaaac tgccggattg tctcttgaat
    11701 atcgggttca ccgtcaaacc ggtcgtaata catcggctgg tcataaatcc ccatcaaaac
    11761 ctcgctgatg ctctggcagt cgcctacctt tgagaatgcc agcgggttat tgcccagcgc
    11821 cagcccagtc tgatagattt caatggcccg ggcgctcacc cgcggcacaa tcggccaatt
    11881 ttgccagtct tcagggggct gccgcaggtc aggcgtgggg gtgaagaccg gcagcgccgt
    11941 tgccgtagcc ggtcgggcgg tctgagtctc ggttgacgct ggcagggtag tctccactgc
    12001 cgggcgggta ggggtttcag gttctggctg ggtggcggtg ggtgcacctg ttgtgctggt
    12061 cggtggcagc actgtgggtg gggtgcagcc tgtcagcagc atcagggtgc ataaaatgat
    12121 aaaataacgg tagaaattcc ccatgggctg ggtacctcga tggttggatt aagaacgccc
    12181 gaattttacc acgcgcccta aatccgtgct tgacagactc cccacgcctg tgtataatac
    12241 ccgcttgcac tgccccggca gaaaacgccg ggcggttgtg tgtattattc ccatcttccc
    12301 cgcagctcgg cggacggtcc gcagggactg tggtgaagcg aagcttggag agcaaaaatg
    12361 aagtatgcaa ttgttgaaaa tggcggcaag cagtatcgcg ccgtcgaagg agccacgatt
    12421 gacgtggacc tcctgccggt ggaagccggt cagcaggtgg atctgtcggt cctcctggtc
    12481 gtggatgggg atcaggttgc agtgggtaca cccgccgtca aaggcgcccg tgtccatgca
    12541 acggtggctg cccatgtaaa gggtccgaag gtcttcacct tcaaatacag ccccaagaag
    12601 cgtattcgca ccaagaccgg gcatcgccag cagtacaccc gtctggttat tcaatcgatt
    12661 gagatggagt agtagcgatg gcacataaaa aaggtggtgg ttccgttcgc aacggacgcg
    12721 atagcaatgc gcagcggctc ggcgtaaaac gctatgccgg tcagtatgtg ctctctggca
    12781 acatcctggt tcgccagcgc ggcacccgca tcaaacccgg cctgaatgtt ggcctcggta
    12841 aagatgatac cctcttcgcg actgcggaag gtgtggtggt ctacatcacg ctgcccactg
    12901 gccgcaagca ggtcagtgtc atgccggttg aagttgttgt tgaacaaccg gctgagtaag
    12961 ttattttgct ccagcaccgt ctgcccgcaa tgagccagac ggaagctgtg agaccccaaa
    13021 gaaaggtatt catctcatga agaagggcat tcaccccaat tattatccgg acgccaaggt
    13081 aacctgcgct tgcggcaata cctggaccac cggttctacc cgcaaagaaa tccgcaccga
    13141 agtttgctcg gcctgccatc cgttctttac cggtcagcag cagcgcctgg tggacattga
    13201 aggtcaggtt gatcgcttct atcgcaaact gcaggctcgc cagtcgttcg ttgaccagca
    13261 gcgcacccgc gaagaatccc gcacttccct ggcccgcccg gttgccgaac ttgaactcgg
    13321 cacccgcccc accgacgccc tcaccaaggc tggcctcacc actgtgggtc agatcctgga
    13381 aaaactccag gccggttccg aagccctgct ggatgttgaa ggctttggcc gcaagtcgct
    13441 gattgacctc aagaagaaac tgcgccagtt gggctatgaa ctgcccgctg ccgccgaaga
    13501 cactgcggtc taatcctccg accccgtttg taagctcatc cctgcgccct caaaagcgca
    13561 gggatttttt atcggcgcat cccggctttc acttccccca aaccccctaa gatgcggtaa
    13621 aatctcatta accttctcca ggagcagact tgcatgaagc atcacaccgg ctctatcgaa
    13681 gttatcaccg ggtccatgtt ttgcggcaaa acggatgaac tcatccgccg cctgcggcgc
    13741 gccaccattg cccgccaaaa ggtacaggtc ttcaaacccg ccatcgacgc tcgctacgcg
    13801 gttgaaaaag tcgcctctca tgcaggcgcc cagtttgatg ccattcctgt caaaacggct
    13861 gccgacatcc tgactcttct ggagcccgac accaccgtcg tcggcatcga tgaaacccaa
    13921 ttcctgggtg atgaaattct cggtgtggcg caaacccttg ccgggcgcgg catccgcgtg
    13981 atcgccgccg ggctcgacat ggactttcgc ggcgaacctt ttggctgcat gcccgcgctg
    14041 atggcgcagg ccgaacgcgt ggataagctc caggccattt gcatggtctg cggcgagtca
    14101 gccagccgca cccaacgcct ggtcaatggc aaacctgccc gctatgatga cccggtgatt
    14161 attgttggcg cctccgaaat gtacgaggcg cgctgccgcc agcaccatca ggtgccgcgg
    14221 aactgaggag aacaacgcaa tgcgtgatta tcgtgaattt ttgggcgaag tcctgatccc
    14281 cgaagaccag ctccaggccc gtgtagccga actgggcgca gaaatcagcc gtgattttgc
    14341 cggcggcgag cctttgctgc tgctctgcat tctgcgcggc ggagtcgtct tcctgaccga
    14401 cctgatgcgc cacatcagca tcccgcacat gatcgaattc atggcggttt cgtcctacgg
    14461 cgtggggcat ttccagtcca ccggctcagt gcgcatcacc ctcgacctca aaaccgatat
    14521 tcagaacacg catgtcatcc tggtagaaga catcatcgac agcggtcata ccattgcctc
    14581 ggtgctcgaa ctgctctcca cccgcaagcc gcgctcgctc agcgtctgct ctctgttaga
    14641 caaggccagc cgccgcgagg tctttgtgcc cattcgctat accggtttct ccattcccaa
    14701 taaattcgtc tttggctacg gtcttgatgt agatgactac taccgcaatt tgccgtttat
    14761 tggtgtggta gatctggaac gttacacgga gggcggcgcc tgaacccgcc aatgccatga
    14821 actttgattg gggcttcccc gaagtattca gtttcttaca ggcccagccg cagcccatct
    14881 acctggtggg cggtgcggtg cgcaatgccc tgctgggatt gaccagccac gacctcgatt
    14941 ttgcagtggc tggcaaagcc tatcccctgg cgcgccgttt ggctgatcac ctcaaggctg
    15001 atgtcttcca actggacgac gtgaacgaca ccgcccgcgt cctcctgacc gcgccagatg
    15061 gcagccgctt ccggctggat tttgccaccc tgcgagctgc cagcctggaa ggcgacctgc
    15121 gcgcccgtga ctataccatc aacgccatcg cgctcgatgt tcacgatctt tcagcgatga
    15181 tcgaccccct ccacggggcc caggatctca aagatcatgt gctgcgcgcc tgctccgatg
    15241 ccagcctgca ggatgatccc ctgcgggtgc tgcgcggggt gcgcctggcg gtgcagcaca
    15301 acctgctggt tgagccgcat acccgtcagt tgcttcatca ggccgccccg ctgctctgcc
    15361 gggtttcggt ggaacgctgg cgcgatgaac tctttcgcat tctcgaaggc cctcgcccgg
    15421 cccaggctgt gcgggttttg cagcagcttg gcgccctgac ggtcattttg cccgaactga
    15481 tcgaactgca gggcgtgcag cagactgccc cgcatgtgct ggatgtctgg gagcataccc
    15541 tggctgcggt ggatcgcctg gagcagctct gggctgtgct ggtggatggc ctgccgcgcc
    15601 ccgaagctga cctgatgatc agcctggcag acatgcgtct ggggcgtttt agggccggac
    15661 tgcgccagca ttttgccgcc cccctggtgc ccgagcgctc gctgcgcggc ctggttttcc
    15721 tcgccgccct gtatcacgat gtggacaagc ccgccgtgcg cagcgtggat gaactgggca
    15781 aggttcattt ctaccgccac gaaaaaaccg gcgctggccg ggccgcagaa cgcgcccgcg
    15841 cactggcgtt gagcgtggcc gaagtgcacc gtctgagcgg aattgttcat aaccacatgc
    15901 gcatccatca catggcgcga gataaccgca accctgaccg ccgggccatc tatcgctatt
    15961 atcaggccgc cgatgtagcc ggtgtggata tttgcctgtt caccctggcc gatacctggg
    16021 ccacctttgg ccccacgctg acgcaggaac gctggctggc cgaagtagat atctgccgca
    16081 ccctgcttga agcctggttt gaaaagcccg aagaggccgt gcgtcccgtc cggttaattg
    16141 gcggcgccga cctgctcacc gagtttaatc ttcaacccgg ccccctgatt ggcgacctgc
    16201 tttacgccgt ggaagatgcc caggccgcgg gagaaattgc cacccgcccc gaggcgctca
    16261 cctatgcccg caactggatc aagttgtatg gcgatctgga agaaggagtc tgacccatga
    16321 attctgaaac agtcccactt tattacgaag aagttggcca ggggctgcca attgtcttgc
    16381 tgcacggcta tccgcttaat cacaccatct ggaagcccgt tgccgacctg ctgcaagacc
    16441 gcgcccgcgt gattatgcca gacctgcgcg ggcatggtcg ctcaccggtg ggcgaggggg
    16501 agccttccat tcgtcaaatg gccgaggatg tctatgctct gctggaaaaa ctatccattg
    16561 accgcgctat tgtagttggg cagtctatgg gcggctatgt ggcgctctca tttgcccatg
    16621 cctacccgcg cctgttggct ggcctgggat tgattgctac gcaagctgag gccgataccc
    16681 ccgagaagcg ccaggcccgc ctgcaaactg ccagcgaggt caaacgccgc agcctcaagg
    16741 gtgtgctggc agccatgccg cccaagatgt ccccacgggc cgacattcag gctgcaaccc
    16801 acgccattat tgccgatgcc tcccgcgccg gcgtgatcta cgccctcaag gccatggcag
    16861 accgccccgc tgctctcgac tggctgaccg aaatagaggt cccggcagtg gtcgtctcag
    16921 ggctgcaaga tgccttcatg ccgcgtgagc gcggcatcac cctggcgcaa atgctgggac
    16981 gcgcctggct ggtggaactg ccagatgccg ggcatatgcc catgttggaa gagccccgcg
    17041 ccgttgccga tgctctgctg caggtgctgg actgcgtcca gaccccgggc tgtcagggat
    17101 aaatctgatc tagaatcggt tcgtaggtgc agcggcagcc gttgtggtgc gagcagcccg
    17161 gtgtgggcag cggcggcacc ttgtctttgt catagacccc ctcgttcgac tggcaaaccg
    17221 ggcagcaatc gttggctacc accacgcgga tctttgtcac gcgcgggttg gcctgcaggc
    17281 gcttcagcgc catcgcatta ttcgaataat cccgcagcgc cgcgccgcag gtggggcaga
    17341 ttaatgccat atcgggggtc tgggtgttgc atcgcgagca ggtttgcatg tttttcctcc
    17401 ctttagttca ggccgctgcc gtggataaga ccgcctggcc aggtttgcgg ctcaccagcc
    17461 gctgaatatg cgccatcaat tcgccgctgg tcaccggctt gacgaggtaa tcatcggcgc
    17521 cggcatccag cgcagtcgcc accatgccgg gctcgtccag cgcagaaagg atgatgatcg
    17581 gcacactgct gaagccgcgg atcgccttac aggtcttcca gccatccatg cgcggcatca
    17641 tcaaatccat cagcaccaca tcgggcgtaa actctcgaac cagttccaag ccttccaatc
    17701 catcgcgtgc ggtaatcaca aaggcgccgc tgggctgtaa taacagcgtc agcagctccg
    17761 tcatagcagg gtcgtcatca acgaccagaa tccgtttaat gtggggtttc ccggcactca
    17821 tagctcttat tatgatataa aacccggctt ttaaaccttg caagtgctta acaagttgcc
    17881 caccgcgctg agagctgttt tctgcaccaa tcattgaacc aaaggtgata ccagatgtaa
    17941 aattcaccct taacctccaa aacccgataa cttttatggt aaaatttatg attagtgtgc
    18001 ttttctacta accgtacacc ccgtacaggg ttcaattctt ttcgtttgag gagttcgtaa
    18061 tgtccaaaaa atgggtctat ttgtttgatg aactcgacca ggctcaggcc tatgtcaaca
    18121 actcgctgga cgcactgcgc ggcctgctgg gtggcaaagg cgccaacctc gccgagatga
    18181 cgcgcatcgg cctgcccgtt ccgccgttct tcaccgtcac caccgaagcc tgcaatgcct
    18241 acctcgccga aggtgagaaa ttccccgccg gctgctggga tcaggtgatc acctccctgc
    18301 ataaggttga agccgccagc ggcaaaacct ttggcgaccc caagaacccg ctgctggtct
    18361 cctgccgctc gggcgccaag ttctccatgc ccggcatgat ggacaccgtg ctcaatatcg
    18421 gcctcaatga tgtgaccgtt gaaggcatgg ctaaactgac caacaacccc cgctttgcct
    18481 acgactcata ccgccgcctc atccagatgt tcggcgccgt cgtcctcggc attgctgacg
    18541 aagcctttga agacccgctg gaagcttaca agcacagcaa gggctacaag atggatactg
    18601 aactgaccgc cgatgacctg aaggaactgg tcaagcagtt caaggtcacc gtgcgccagc
    18661 acatgggctt cgatttcccg caagacccca tcgaacaact ccgcctggcc actgaagccg
    18721 tcttcaaatc ctggaacggc aagcgcgcca tcgattaccg ccgcgccgaa ggtatccccg
    18781 ataatctcgg caccgccgtc aatattgtga ccatggtgtt cggtaacatg ggcatggact
    18841 ccggtaccgg cgtggccttc acccgcaatc ctgccaccgg cgagcgcaag ctctatggcg
    18901 attacctgct caatgcccag ggcgaagatg tcgtcgcggg catccgcaat acggatcccg
    18961 tccagaagat gggcgagacc ctccccgaag cctacgagga gttcctcaag atctgcgcta
    19021 tccttgaaaa gcactacaag gacatgcagg acatggaatt caccatcgaa cgcggccgcc
    19081 tgtggatgct gcaaacccgc aatggcaaac gcaccgccag cgcggctgtg cgcgttgcgg
    19141 ttgaaatggt tgccgaaggc ctgattacga aagaagaagc cctgctccgc gtcaccccca
    19201 agcaggtcga tgccctgctc cacccgcaat ttaacgacac tgccaagaaa gctgccaaga
    19261 aagaaggccg cttcttcgcc agcggcgtga acgcctcccc cggtgcggct gtgggtcaga
    19321 tctactttga tgccgatacc accgaacgca tggccaaaga atccggccag gcggtcatca
    19381 tggttcgccc cttcaccaaa cccgatgatg ttcacggtat gctggctgcc aagggtattc
    19441 tcaccagcga aggcggcgcc acctcccacg cggctgtggt tgcccgccag tttggcgtgc
    19501 cctgcattgt aggcgcctct gcggtcaaaa ttgacctcga gcgccgtgtg atgcgtgtgg
    19561 gcgatgtgga agtcaaagaa ggtgaatgga tttccgtgga tggcggtacc ggcgaagtct
    19621 atctgggcaa gctggaactc accgtcccga atctggaaga acagaaggaa ctgatgaccc
    19681 tgctccagtg ggcggacgaa gcctcccgcc tgcaggtctg ggccaacgcc gactatccca
    19741 aagatgcccg tcgcgcccgc tcttacggcg ccaagggtat tggtctgtgc cgcaccgagc
    19801 acatgttctt tgaaaccgtg cgcctgccca tcgtccagcg catgatcctg gccgaaacca
    19861 gcgccgagcg caccgccgcc ctcgatgaac tgctgcctta ccagcgcgct gacttcgatg
    19921 gtttgtttga ggcaatggat ggctatcctg ttatcatccg cctgattgac ccgccgctgc
    19981 atgagttcat gcccgacgaa gaaaaactcc tcgaagaagt catcgccatg cgcatcaagg
    20041 gcgaaaccaa gggcctgccc gagaaggaaa aactgctggc ttccatccgt ggcatgcacg
    20101 aaagcaaccc catgatgggt ctgcgcggcg tccgcctcag catctacatg cccgaaatcg
    20161 ttgagatgca ggttcgcgcc atcttcgaag ccgccgctga ctgcaccaag cgcggcatcg
    20221 tggtcaaacc cgaagtgatg atccccctga ccggcaccgt tgccgaactc aagtggatcc
    20281 agccccgcct tgtgcgcatt gccaaggctg tcactgaaga gaagggcgtt tccttccact
    20341 ataaattcgg caccatgatc gaaatcccgc gcgccgctgt cactgcggcc gagatcgccg
    20401 gcctggccga gttcttctcc ttcggcacca acgacctcac ccagatgacc tttggctaca
    20461 gccgcgatga tgccgagcgc aacttcctca ttaagtacgt tgaggagggc atcctgccca
    20521 agaacccctt ccagaccctc gaccgcgatg gcgttggccg cctgatgcgc atggccgtgg
    20581 aagatggccg ccgcgcccgc cccgaccttg aggtgggtat ctgcggtgag catggcggcg
    20641 atcccgactc gatcgagttc tgccacctca tcggcaacaa ctacgtcagc tgctcgccgt
    20701 tccgcgtccc ggttgcccgt cttgcggcag cccaggccgc catcaagcac ggcaagtaat
    20761 tcgcctgcca aacaaccaac cacagcgccc ggctttaagc cgggcgctgc ttatatcccc
    20821 atatctaacc tttcccctcg cactttattt tctttttcag tttgcctttg gtataatctt
    20881 gatagtttga gtgaacaata tcagtaattc cgtcattcaa ttgacgcatt cttcaaggga
    20941 gctttctatg gataaagcgg aacatctaaa actatacaaa gaaatggtgg tcattcgccg
    21001 ggttgaggag cgcgctgccg aactttacca gcagggtaaa attggcggct ttctgcacct
    21061 ctatatcggg caagaggctg tcagcaccgg gcttgtctcc gtccgccagc cggtggaccg
    21121 ggtgatcaca gcctaccgtg accacggcgt ggcgatcaac tgtggcattc cggcccgcca
    21181 ggtggttgcc gaactgcttg gcaaggccac cggctgctca ggcggcaagg gcggatccat
    21241 gcacatggcg gatgttgaaa agaacttctg gggtggtcat gccattgtcg gcgctcacct
    21301 gccgctggcc tcgggcctgg cgctgggtga tgtctatgct ggcaacaagg ccgtcaccct
    21361 ttgtatgttt ggcgatggtg ccaccaatat cggctatttt cacgaggcgg ttaacctggc
    21421 caaggtttgg aacctgcccg tcctgtgggt ctgcgaaaac aatcaatatg gtatgggcac
    21481 cgccgtggat cgggcctcgg cagtctccga gattatcgcc aaggcctgcg gctacggcat
    21541 gcgcgccgaa caggtggacg gcatggatat catggcggtt cgccgcgtgg ctggcgagat
    21601 gatcgccgaa atccgcgccg gcagtggccc ccagcttctg gaggtgttga cctaccgctt
    21661 ccgcggccat tccatgggtg accccgagcg ctaccgccag gcttccgagg tgcatcaatg
    21721 gcaggaaaac gaccccatcg gcatctaccg taaatacctg gtcgataaca acatcgccac
    21781 caccgccgaa ctggatgaac aggatgacct ggcagaagcc gaggtcactg acgcggttgc
    21841 gtttgccgaa tccagcccgg aaccggcacc tgaagaactc ttcaaacata tctacgtcga
    21901 agaataggag cctgcaatgg caaaaattac catgcgcgaa gccatttccc aggctttatg
    21961 ggaagaaatg gaacgagatc cccgggtctt catcctcggt gaagaggtgg gcgtctgggg
    22021 cggcacctat gccgtgacca aaggctttta cgatcatttc ggcgccgagc gcgtccgcga
    22081 tacccccatt gccgaggcgg cgattgttgg cgcggccatc ggtgctgccc tcaccggtca
    22141 gcgccccgtg gctgaactga tgaccatcaa cttcgccttc tccgcgatgg atcacatcgt
    22201 caacgaagcc gccaagatgc actacatgtt caacggacag atgcgcctgc cgctcgtcat
    22261 tcgcaccgtc ggcggcggtg gacgccagct cggcgccacc cactcgcaaa cccccgatgc
    22321 catgttcgcc catttcccgg gcctcaaggt ggtggcgccc ggcaccccgg cagatgccaa
    22381 gggcctgctc aaagcggcca ttcgcagcga agaccccatt ttcttcatcg aacatgccac
    22441 gctctaccag acccgcggcg aagtgcctga aggcgatttt gtggttccca ttggcaaatc
    22501 caccatccag cgccccgggc gagatgtcac cattgtgacc tacagcaaaa tgctggaact
    22561 gtcgctcaag gctgccgaac agttggcggc tgaaggcatc gaagccgaga ttgtggacct
    22621 gcgcagcttg cgcccgctgg acatggaccc tgtccttgaa tccgtgcgcc gcacccaccg
    22681 cgccgtaatg gttgaagaag gctggaaatc ctttggcgtc ggctccgaaa tctccgcccg
    22741 tattcacgaa gaagcttttg atgatctcga tgccccggtc aaacgtgtgg cgcagcttga
    22801 agtgcccctg ccctataacc gggcgctgga gcaaatggcc ctgcccaaag tcgaacacat
    22861 catccaggcg gtcaaggagg tgctctaatg gccgataccg tctccatgcc caagctgggc
    22921 tttgatatgg ctgaaggcac cctggtgcgc tgggtcaagg ctgaaggcga agcgattaac
    22981 aaaggcgatg tgctggccga aattgaaacc gacaaggcca ccgttgaggt ggaatcgggt
    23041 ttcggcggtg tggtggcacg tcatctggtc gcccagggca gcattgtgcc cgtcggtgca
    23101 cccattgcca tcatcgccgc ccccggcgaa actgtgactg atgccgcccc cgcggctgct
    23161 ccggcagaaa agcccgccgc ggccccggct gccgcgccca ctccggcacc tgcccccgcg
    23221 gccgaaattc cccccgccgt gggtgggcgc gtcaaagcct cgccgctggc ccgccgcatg
    23281 gctgccgagc gcggcctggc gctcgaaggg ctgcgcggca ctggccccgg cggccgtatt
    23341 gtcaagcgcg atgtggaagc cgccgcccaa acgcccccgc cggcgccggt catccccacc
    23401 gccgcgccgc tgtctgtaac ccagccccac gctgccgagc aggtgccagt ggaccgtctg
    23461 cgcgctgcca ttggtcgccg cctggttgaa tccaagcagc aggtgccgca tttttacatt
    23521 acccatcaat gtgacgccgg cccgctgatg cgcctgcgca aagaggttaa cgaaatgctg
    23581 cccgaggatg ggcggctctc ggtcaatgat tttatcgtca aagccgctgc gctggccctg
    23641 cgccagttcc ccaacctgaa tgcctcgctg cagggcgatg tgatcctgcg ccacggagca
    23701 gtcaatattg gcgtggctgt ggccgttccc ggcggcctgc tgactgtggt gtccaaagac
    23761 gccgaccaga agcccttgcg ggtcatctct gccgaagtgc gggagatggc agcccgcgcc
    23821 cgcgccggca aagtccgccc ggaggacatt gaaggctcca ccttctcaat cagcaacctg
    23881 ggtatgtttg atgtcgagtc ttttgtcgcc atcatcaacc cgcccgaggc ggccattctg
    23941 gcggtgggtt cggtcaagga aatccctgtg gttgtggatg gcgttgtcca gcctggtctg
    24001 cgactgaatg tgaccctctc tgctgaccac cgcatcaccg atggtgctga ggcggcccaa
    24061 ttcctgcaag ccctggccga attcatcgaa aaacctgccc ggatgttggt ctagaattac
    24121 tttctggcac ctttgaacaa aatttatttg ggcgggtcca atgacccgcc caaatgattc
    24181 tgatcacgtt atcgggtaga actgcgcaaa ttcgggaaca tcacggctcg cgggcgtaca
    24241 ggctggcgta aatctcaaag ttttcaaaca cccgccgcac ataatcgcgt gtctgatcgt
    24301 agcgaataca ttccagatac aggtcggggt ctgtgccaga aagctcgctc caactgcggg
    24361 cattgcctgg cccaccgtta tacgctgcca gcgcaccgta atagtcgctg ttcaggtaaa
    24421 tatactgtcg ggacaggtac cgcgtgccca tccggatatt gactgccggg cggtaaagat
    24481 catctgtcgt atagcctacc ggccagctca ttgaagtggc tatctcctgc ccggtggctg
    24541 gcatgatctg catcaacccc cgcgcccccg cactggattc ggcaaacccc tcaaagaagc
    24601 tctcctgccg gatcaccgcg aagaccagca gcggcggtac gccttcctgc gctgcggcgt
    24661 ccagcaccag atccttgtaa taggcgccga accgaatgtg gttgaaataa gccggtgctt
    24721 ccagcgtacc cgcgtcatcc agcccggcca aatccagcac ctgccgcgct gagagtatcg
    24781 ccgagcgata cagcccgcgc tccaggaaaa agttcatcag ccggtaggtg gccgtggcat
    24841 cgttgagcac attcaaccgc agcgtttcat actcaccccg tgccgagcgg tataaaccca
    24901 gttcccacag ggcattgccc ttgaccatcc gagggtccgc cgccagcgca tccaacccgc
    24961 tcaaatctgt ctctgccggc aggccaaaag tcacccgcat ccatgtatcg gcctcaaccc
    25021 gttcgctggt caggtcgtag gccagatcat aaaccgctgg ttgtgtgaag ggcgcctggc
    25081 ccgccagcag ttgggctgcc cgctcagagt aatacccggt agggtcgcgc tgggcagcct
    25141 ctttccatga ggcctgtgcg gctgctgcat cattctgcgc cagttggcac ttgccaatcc
    25201 acaggtaagc catagcctgg tcaccgggct cacccgccag caccagcacc cgctggaaga
    25261 ccagcagcgc ctggggataa ttccccaggc ggtagtacgt aatcccggcc agaaacaggc
    25321 cgtaataact gtcttccgcg tttggatatt cgttcatcag ccgttcccag gcctcggcgg
    25381 cctggctcag atagcccccg cgctccatga tctgcgccgc ccgaaaaagg aaccccggcg
    25441 cataatccga gccgggcacc ttctcaatat aatccagcag aatttgagcc ccgcgggtat
    25501 agtcacccag ccagtaccag taggtatcag caatttcatc atgggcatcg gcccagaaac
    25561 ggtcaccact gtggtcctgg atcagtgcct gccattcggc aatcgcctgt tcgggttgat
    25621 ccagggcgcg ctggcagagt gctttgtaat gatgcaccgc cccgtcatgg gtgggattgg
    25681 ccttcaggta acggtcaaag gcctcaatgg catagccgta ctgcccggcg taataatcca
    25741 ccaacccgcg gttgaactcg tctaccggtt ggtcggcatt gaccagcgcc accagcccgg
    25801 tgtaggtatc gtaagaagtt gggaaaaaca gcaccgactc ttgatagcgg gcataggctt
    25861 gttccggcag ccccagtgcc atataggtct gccccgccag caggttggcg gttgccttgg
    25921 tgtaatcgct ggcggtgctt tcgtacacag ccgtgaaggt gcggatggca ttggtataat
    25981 cgctcactgc ggcataggcg ctgccttttt tgatctgcaa gtcatctgta atcccactgc
    26041 tcgcggcaat ggcggcatca taggcttcta ccgcggccag gtaatccccg gcagcttgca
    26101 gggcatcgcc gcgcttttcg tacatcatcg cgtccagcac gcccgggcgc agttgaatat
    26161 agcccaggta ttcctgtgct gccagcgccg gcagcccggt ttgcatatgg cactcggcaa
    26221 tctgataata tgccggtgct gcagcaatcg cctgggggta ctgcgtcagc agattttgca
    26281 gcacactcag cgccgaagcg cagtcgccgt tgatggcata ggtttgcccc acccctaaca
    26341 gagccgcagc ctgggtttcg gtgtcgggtg ccgcattcag cgccgcctga aattcttgca
    26401 gcgccgccgg ataatcgccg gtcagcagca gcgcatcgcc ctgttccacg cgcaccgttg
    26461 gcggcacggt gggggtgggt gtgggggtcg gctgtggcgt ggcggtgggc gccgtggttg
    26521 cggttggaac cagtgttggc tcggcctgat tgaatggcag gttgcaggcg gtcaatagca
    26581 gggtgaacag caagaagatg atgatttttt tcattatatt cctccatcca tagcgggtgg
    26641 ttaagttgat tatactgtaa tgagacagcc aaaccagaat gtactgagat ttccgcactt
    26701 ccctgtccag tcaacctttt gacaatttag ggcttgcatg ttaaacttcc tcttgcattt
    26761 caaccccggc gctctgcgac acacagggcg tctttttgtg cgaggactat gctcacgctc
    26821 tggtgggatg acaccacaga aaccgtccag atgatcgatc agcgggcgct gccggcccgg
    26881 ctggaaatat tgtcctgccc cgatgccgct gctgttgctg aagccattcg caacatgact
    26941 gtgcgcggcg cgccggcgat tggtgttgcc gcggcctacg gcctggcgct ggctgcctgg
    27001 cacagccccg ccaccgaccc ggctgacctg cggactgaca tcatgcaggc ggcggccatg
    27061 ctgcgcgcca cccgccccac tgcggtcaac ctggcctggg cgctcgacca aatgctgcgc
    27121 atagccgata cgcctggcct gacggttgac aagctgcgct accgcctgct ggctgaggca
    27181 cgccgcctgg ccgaggaaga tattgccacc aaccgccgca tggccgaatt tggcgccgca
    27241 ctgattgacg acggtgacac catcattcac cactgtaaca cgggctctct cgccgtggta
    27301 gattacggca ccgccctcgg tgccatccgc cgggcgcatg agcagggcaa gcgcatcttc
    27361 gtcctggtgg acgagacccg cccccgtctt cagggcgccc gcctgaccgc ctgggaactg
    27421 acccagtacg gcatccccta tgccatcatc ccggatggcg cgtccgggca ttttctgcgc
    27481 cgcggcgagg ccaacaaggt cttcttcggt gccgaccggg ttgccgccaa tggcgatgtt
    27541 gccaataaga tcggcaccta catgctgtcg ctggctgccc acgccaatga tgtgcccgtc
    27601 tatgccgtga tgcccgcctc taccattgac ctcagcctgc cggatggcag ccacatcccg
    27661 attgaagaac gctctgccga tgaggttctg aacctcacca ttcacggcga gcccatcgcg
    27721 ccagccggcg ccgcggcgcg caacccggct tttgatgtca ccccccacac gctaatcacc
    27781 gccctggtca ctgaccgcgg cgtggtttat cccccgtttc acatccatct gccccgtcta
    27841 ttgaacggga ctcaatctgg ttcactctga gaggtttgcc atgaccattg tttgcaccgg
    27901 ttcaattgcc tacgactacc tgatgacctt ccccggttac ttcaaggatc acatcctgca
    27961 ggaacggctg gactgcattt ccctctcctt cctggtcgat tcgatggttc gccagcgcgg
    28021 cggcaccgcc cctaacatcg cctataccct ggcgctgctg ggggaccgtc cgctggtctt
    28081 tggcaccgtt ggcgaagatt ttgaagatta ccgcacctgg ctcgaaagca aacatgtgga
    28141 taccaccttt gcccgtctca ttccgggggt tttcacagct tcattcttct gtaataccga
    28201 ccgctccaat gcccagattg ccagctttta ccccggcgcc atgggctttg ccagccagtt
    28261 atccctggcg gaactgaccc agtacaaggt ggacatggtg gtcatctcgc ccaacgaccc
    28321 gcaggctatg gatcgctgcg tgcgcgagtg tcagcagatg ggtctgccct acctttatga
    28381 ccccagccag caggtggtgc gggtcagcgg cgaagaactg cgccgcggtg tacagggcgc
    28441 ctacagcctc ttctgcaatg aatatgaatt tgaactgctg caaaaacaca ccggtttcag
    28501 tgccgatgag atgcgctcat tggtcaagat tctggtggtc accctgggtg ataagggcgc
    28561 atccatcttt accgccgggc aggaaatcac cgtgccggta gtccccgccc gcgaaattgt
    28621 ggaccccacc ggtgtgggcg atgccttccg gggtggtttc ttgcgcggct ggcagcttgg
    28681 cctcgattgg gccacctgcg gtcagatggg cgcgctggca gccacctacg tcctcgagca
    28741 gcgcggaccg cagagccaca gttacaccct tcccgagttt gtagcccgct atcgccagca
    28801 ctttgacgat catggcgccc tggatgtgct gacgcgctga aatccaatca tccagcgcca
    28861 aacgtggaac atatcaaaga atcgcgatac aatataccgg cttttggcag aaccccatct
    28921 ggggctgcct tgccccggtt caaaattttt taggaggaag taatgaccca gtacgatgta
    28981 aaagatctct ccctggctga aggcggacgc cggcggattg agtgggctgc ccgtgagatg
    29041 cccgttctac ggagcattag cgagcgcttt gccaaagaac gccccctggc gggcatccgc
    29101 atgtcagcct gcctgcatgt gaccgctgaa actgccaacc tgatgcgcac cctgcagctt
    29161 ggcggcgctg aagtggttct caccgcttcc aatccccttt caacccagga tgatgtggcc
    29221 gccgcgctgg tctcattctc agaaatcccc acctacgccg tcaaaggcga agacaatgtg
    29281 acgtattacc gccacatcaa tgccgccctc gatcatcgcc ctcacatcac catggacgat
    29341 ggcgctgacc tggttggcac catccacaag gatcgccgcg atctgctgtc tgagattctc
    29401 ggcggcaccg aagaaaccac cacgggcgtc atccgcctgc gcgccatggc tgccgacaag
    29461 gccctgatgt tccccgtcat cgccgtcaac gacgccatga ccaagcactt ctttgataac
    29521 cgctacggca ccggccagtc taccattgat ggtattgtcc gcgccaccaa tgtcttgctg
    29581 gctggcaaag tcttcgtcgt cgccggttac ggctggtgcg gccgcggcct ggccatgcgc
    29641 gcccgcggcc tgggcgccaa cgtcatcgtc accgagatag atccgcttaa ggcactggaa
    29701 gccgtgatgg aaggctaccg cgtgatgccc atgatcgatg ctgcccgtat tggcgacatc
    29761 ttctgcaccg tcaccggtga cctgaatgtg ctggatcgcc atcactttga ggtgatgaaa
    29821 gacggcgcca tcatctccaa ctcgggccat ttcaatgtcg aaatcaacat cccggccctg
    29881 gctgagatgt cggttgagaa gaaactggtg cgcccctttg ttgagcagta cgtcaccaaa
    29941 gatggccgct cgctcttcct gctgggcgaa ggccgcctca tcaacctcgc ctcggccgaa
    30001 ggtcaccccg ccagcgtgat ggatatgtcc tttgccaacc aggccctggc tgccgaatat
    30061 atggtgcgca acggcaaatc gctggagcgc cgcgtctaca gcgtccccga agaaattgac
    30121 cacgaaattg cccgcatcaa gctgagcgcc atgggcattc agatcgacac cctgaccccc
    30181 gaacaggttg cttacctcgg ctcctggcag gaaggtacat aagtttttcg cgcattaacg
    30241 tgcgccgcta aaactcacca cgggtatgct gccaatcgtt tttcgctgca tgcccgtggt
    30301 gaaaatatta tccagaaagc ctgctgctta tgaaacgttt ttttgctctt ttggctgttt
    30361 tattcctggt caccctggcg ttagccgcct gtaccacgcc cacgcccgcc ccgaccttca
    30421 cgccggcccc cacggatact gccacacccc aaccctcggc aaccctgctg cccaccctca
    30481 ccgatacccc cacaccgctg cccaccgcca cgctgacggc taccgccacc tggacgccca
    30541 cccccacgcc agacttcagc caggtgcgca tcactgcgca ggattatcgc gccgccacgt
    30601 caaattatct gattatcctg gccttccccg gtattaaaga gccctacctc acccgcttga
    30661 acggcaattc ctataactgc gttctggatg agaactatcc cgatcagtat ttctgtgttg
    30721 gccccatcac cggctacatg aagccagtgg ctgccgagtt cacccgcccg ggcgagacca
    30781 ccccgttttt caccggctac attggcggct atcccttccc ggagccaacc ccgcttcccg
    30841 taggtgcgcc cgatacctgg tgcccgctgc gcggtcagca catcacctgc gagccggaat
    30901 attacaccaa atatgattgc tggattacca cctgttacga cctctgcggt tactattaca
    30961 gcgtggatac ctgcctggct gtgaccccca cagttgggcc gtaatgattg acaaggagat
    31021 gaaaatgcgc aaattgacct ggatgggttt gttgatcctg gtggccatga ttctggctgc
    31081 ctgcaccccc gcccccaccg cagtgcccac cgccctgccc accgccacca acaccgaaac
    31141 cctcgccccc accgaaacac ccgtacccac cgatacaccg gttcccaccg ccaccaatac
    31201 ccccaccctc acccctacca tcacgcttac ccccaccgcc acccccgatt acagcgtgat
    31261 tcaggtgatc tggcaggaaa gctacaccaa ccgccagccg attgttgccc tgaagatccc
    31321 cggcattacc gaagcttacg gcgccaaaat caacaacgtg gcttacacct gttcaattga
    31381 tgccaacacc cccgacacgc tgttctgcaa cggtccgctg ctgccgtact tcacgtacgt
    31441 ccagttggcg ctgtatcacc ccggcgatgc cctgccgttc tacgaagcca ccatccctgg
    31501 cccggtgcgg gcgctgccca ccgccacccc ggtaggcaac cccaacacct ggtgccccga
    31561 tcgcggtcag aacgtccact gcgagactga aaaccgcacc taccccggcg gcttgcagtg
    31621 cgtggtctcc acctgttacg atgcctgtgg ctactactac agcgtggata cctgcccgac
    31681 aaccaccccc aacccctgat tcagccggta gatagagaaa taaagaggcg aaccatcatc
    31741 ggttcgcctc ttctgattgt atctggcgca ctcactcacg gcgacagggt aggggtgacg
    31801 cccgcctcgg gttgaatgac cgtaaagtca atgaaaaacg ggtcgccaaa tggcagccca
    31861 tccgggttgt gggcctgcca ggcgctgcgg taatcgcccg gctccagcgg tgccgtaaat
    31921 acaatccgga tcaccgtgtt ggtgccgctg cgggtgggcg tcagcgcctg ttcgctgctg
    31981 gcgcccagtt cttccccggc aatcaggcgc agccggtaat tctcattcca gttgcacgta
    32041 ccgctgttct gcacctccca gcgcttatcc agcgtggagc ccggttgaac ctggctgccg
    32101 tctggaatcg tcacatcggc gatgaagctc aacaggtctt cgcaggggcc gccgggtgtg
    32161 gcagcttgtg gcacagcctg aacactctct gttgtggggg tgggtggaat ggccgtgggc
    32221 ggcacaaaca gcggcgcatc ggcctgaggg gcctggcgct cgggctgcgg caggctgcag
    32281 gcggttagcc ccagcagcaa aaccactgcc atcattccca accacgaaaa gcgtttcagc
    32341 gcacgcatcg caggcggttt attctccgtc gtcctcggct tcaccgtcgc cgccgcccag
    32401 ggtgatcacc gcttcgccgc tgagcacctg ctggcggatg gcggtttcga tctcattgga
    32461 cagcgccggg ttctggcgca ggaactcttt ggcattttcg cggccctggc ccaggcgctg
    32521 ctcgccgtaa gagaagaacg cgccgcgctt ggtcacaatc tccaactggg tccccaggtc
    32581 aatgatgtcg ccgaccttgg aaatgccttc attatacata atatcaaact cggccacacg
    32641 gaagggcggg gccaccttgt ttttgacgac cttcacgcgc acccggttgc cgatgatatc
    32701 cgcgcccacc ttgatggatt ggatccggcg cacatccagg cgcaccgagg cataaaattt
    32761 gagcgccata ccgcccgagg tggtctcggg gttgccaaac atcacgccga ttttctggcg
    32821 cagctggttg gtgaagatca ccgccgtttt cgtctgattg atggcgccgg acagcttgcg
    32881 cagtgcctga gacatcaacc gggcctgcat acccatcgag gcatcgccca tgtcgccttc
    32941 aatttccgcc cggggaacca gcgccgccac cgagtcgatc acgatgatat ccactgcgcc
    33001 cgagcgcacc agggtttcca caatttccag cgcctgctcg cccatgtcgg gttgggcaat
    33061 cagcaggttg tcaatatcca cacccaggcg ggtggagtaa gccgggtcaa gggcgtgttc
    33121 catgtcaata taggccgcgg tgccgcccat tttctgcgct tcagccacag tgtgcaggca
    33181 cagcgtggtc ttgccagaag attctggccc gtagatttcg gtaatgcgcc cgcggggcag
    33241 cccgccaacg cccagcgcaa tgtccagtga gagcgacccg gtggggatca cctccacggc
    33301 cagttggtgg gcttcgccca tgcgcataat cgagccttcg ccgtagcgct tgacgatatc
    33361 acccagggcg cggtccaaca cggcccgccg gcccgggtcc atagatgaat tttgagctga
    33421 agattcagca ggcggggagg atttacgcgc catgtttttt ctcctgttgg tcagtatacg
    33481 tttattatat ccacaattgg tttgcgcatg gagcgcacaa ctgtcaaaaa ttatagtaca
    33541 tttgttcggt ttgtcaagtt tttgagccca tcagcttgca gaacccgcac acccccgcac
    33601 tggtggtcgg ttgaccacag tttgggcagg ggtgcagccc ggcgcggtct gcctcttgca
    33661 ccgggcccgg aaacagcgcc gtccgtgcct gcaaaaactg aacgtagaac tgcatcttgg
    33721 cgccgggccg ctcggcttcc atgcggttga gcacggtttt gtaatacagc agcgtgctgc
    33781 cctccgcaaa aggacattcc tcttcaatgt attcgatccc ttgcagcagg gcataggcgg
    33841 cagtctcgcg ctcataaaag cggcagaaag gcttggcctt cagcggcagc ccatcatgcg
    33901 cttccagccg gggcgcctgc cgcgccagca tcggcactgc ccagttcatc gtgttgaggt
    33961 acagcaccgc ggcttcgtca tccaggttgt gggccgtcat caagacatca tatcgccccc
    34021 ggattgcggc ttcgttcata atgtgccgct tggtctgccc gcagatggag cagggcttgt
    34081 accgcccgcg cgcgctctgc cccgccaggt cgggaatgct ctgcccgtac cgttcggtca
    34141 cgttgaccac ctgcaagctc agcccgcgca cggcggcaaa gtcctcagcg tagcgctgtg
    34201 attgcgtgga gtaatccccg tctccggtta tccccagatg gatatacatg ccatcagcgg
    34261 cataccccag ccgccagagc acatcccaca gcgagagcga atccttgccg cccgaaaccg
    34321 ccaccagaat ccggtcgccg ggccgcgcca gttgatactt cttgatgctc cgggccgttt
    34381 gagcgataaa ccagtcaatg taatcctgcc ggcacagcgc caggtggtgc tgcggcattt
    34441 tgatgacggc gcgttcgccg cattttttac acttcaccgg ctgccccccg aaatcaccgg
    34501 gatcaatttg atcacgtcgc catcgttcag gcgctcatct tcggtcagca gttcgccatc
    34561 gcgcaccgcc aggtaggtct cgggcgtcac gcccaccagt tccatggcgc gtttcaccgt
    34621 cggtgcgtgg ggcaaaatat gttcttgctt gcggagaata atggtgacag ccaagttcag
    34681 ctccagggtc agggtttttg ttcagccagc catttgcggg ccagttcttc actgtcaaac
    34741 ggtgtgaagt tgcgtccaga gaccacctcc acacctttca gcagcatttt cataaaacta
    34801 tccagcccaa ccacagccgt agccatggca aacggcgcgt tttcagtcgc cgtatctttc
    34861 agcgcccgca cggtgtctac atcaatatgc gggcggtaag ccagcaccag aatccgcagc
    34921 gagcggggag gctggctttg gatcatcttg cgggccgagg cagccgtttg cagcagctct
    34981 tcgtgtgagg tctccgaaaa atcctccacc aacagttgaa tatcctgaaa aatctcaaac
    35041 cgaactcgct ctgccacagc acacctcctc cgtttgggtc agtcgctgcg ggtgccatcg
    35101 ggcagcgtgg cccccgtcag tgatttggct cgcaggatgc ggtaatcggg cacattggcg
    35161 cgcagcaaat tggccccgct caggttggca ttggtcaggt cggcgccgcc caggttggca
    35221 tccagcaggt tggcgcccgc caggtcggcc tcatagaggt ccgcctcagc caggttggcc
    35281 tcgcgcaggt cgcagttgcg caggttggcg ccgcgcaaat tggccttgcg caggtctgcc
    35341 cccacaataa ttgagccttg cagcgtggcg ccgcccaggt tcgcctcgcg cagatccacc
    35401 ccttccaggt gtgtgccggt catatcggcc ttaatcaggg tggccccgcg caaatccacg
    35461 ccgcgcaaat ccataccact caagttcagg tgcgagagcc agaggggttt gggctggttg
    35521 agcagcatat acacttcata ccgtgaaaac tcggccatgc cgcactcctt ttctgccaga
    35581 aagccagtgg acgtttattt cattgtaatt gaaaaaccaa agattacaag tagcgcgcgc
    35641 cggactctat tggcctaa
//
LOCUS       DAOPVS010000012        34953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1167_length_34953_cov_53.298155, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000012 DAOPVS010000000
VERSION     DAOPVS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 34953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34953
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1167_length_34953_cov_53.298155"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKL11_02455"
     CDS             <1..661
                     /locus_tag="PKL11_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNZ12489.1"
                     /translation="LHLISKLRHDAALYMPYLGPKPKRGPTPRLGEKVNVRQLPDTYL
                     KETEIKDGFETRTYQLPLLLSEFAQPLNIVILLRTNLTTKACSHVILFSSDLELAHDK
                     LIDFYSLRFQIEFNFRDAKQFWGLEDFMNVSEVAVTNAANLSLFMVDISQVLMCEYRQ
                     NDPDFSILDLKAHYRGYRYVSETIKMLPQMPDSNLVLQLFSKVAALGRIHPAKLPVSS
                     P"
     gene            751..3603
                     /locus_tag="PKL11_02460"
     CDS             751..3603
                     /locus_tag="PKL11_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNZ12490.1"
                     /translation="MKFRPFLAVAMLAILLTSFLTAWSPAAPVALVSEKVAPLLLQRI
                     AAEGHTEYYVIMAQQADVSAAANLPTKLEKTTYVFNALTAMAEKTQKPLLTQLEKEGV
                     SYQSMYIQNMIKVDSGITTLTWLSARPEVAKIVVPPTPVMDPVIKEMTPEQKTKAIEW
                     NIIRIGADDVWGMGFTGNGLVVASNDTGVEYTHPALVNHYRGTTGPGTYDHNFDWWDG
                     TTGYDYPHDYDGHGTHTTGTMVGDDGGTNQIGVAPGAQWIACPGIGGGGTDTVECFEF
                     FLSPWDLNRANANPALAPDAINNSWYDPSGYDYRTIIQNLNDAGVAVIKSAGNQGSAC
                     NSISNPGYVPEIISTANFAQGDVISSSSSRGPSSYYGETILKPEVAAPGTNIRSSVPG
                     GGYEGGWSGTSMAAPHTTALIALMWQAAPCIMGDVPTTKTILQDTADEKIDAQCTPFV
                     GHPNDVWGYGILDSVEAVNAAIAFCGGLGTLDGTVTDASTTNPLEGVTVNAVETGGFN
                     RTIATNASGYYTAELIEGSYTVTASLYGYETATITDVAVTADTTTTQNIALTPAEIFT
                     LSGVVTDAVSTAPLQAAITILDTPLAPVTSDPGTGAYSIDLPAGTYMLRAEADAHLTL
                     EMEITISADLTQNLALEPVPCILLVDDDAGNSYETYYTAALDALGLDYALWSTSGGSP
                     TLADMVDYTMVLWFTGNDYSTTISSTEEADLAAYLNQGGNFFLSSQDYLYDVGGSTTF
                     DTTYLQIQSFTSDVNKNNPVGVAGDPIGDGLGAYTLTAPSGFGTLYTDNVVKTATAGV
                     TNPFKWNDIANFNSTSYDSGTFKTVFLAWPIEGLSALADRQAVLGAVVDFFGGCTTCE
                     PITNADFTWLPIDPAAGEAVAFTSTIATGDGTITYSWDFGDGSTSDVQNPTHTFADGG
                     DFTVVFIASNTCSNSNRTEIVSVIPFVKIFLPTILR"
     gene            complement(3741..5210)
                     /locus_tag="PKL11_02465"
     CDS             complement(3741..5210)
                     /locus_tag="PKL11_02465"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNZ12491.1"
                     /translation="MKTFRNYIDGTWVASKAAETVDVINPATGEVLWRTPLSTAAEVD
                     AAAQAAAAALPAWRQTPAQDRIQYLFKLKALLESNLEEIGRTITMECGKTVEEAIGEM
                     RRAIENVEVACGIPMMMKGEIAEDIAPGLDEIMLRQPVGVCATIAPFNFPGMIPFWYL
                     PYALACGNTYVLKPSEKTPMTMQLIFTLIEQAGLPKGVVNLVNGAKEAVDSILDHPQI
                     RAITFVGATETARYIYRRAAENGKRVQAQGGAKNPIIILPDADMDMAVKITADSAFGC
                     AGQRCLATSLAITIGEARRPFVERICEAAANRRVGNGLEPGIQMGPVINSASKERIER
                     LISQGAAEGASLPVDGRRAVIQGYENGYFVRPTILLDLPLGGEVARTEIFGPVLGVAH
                     MDSFDEAIAVINRGTYGNQASLFTSSGSAARKFRYEVQAGNIGINIGVAAPMAYFPFS
                     GWKDSFFGDLHGQGNDAVEFFTQKKIVVERWPKAWSRMF"
     gene            5286..6026
                     /locus_tag="PKL11_02470"
     CDS             5286..6026
                     /locus_tag="PKL11_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNZ12492.1"
                     /translation="MTEIQSLARGLKILSLLSQSPDGISITELAEYLDVDKGSASRLV
                     STLSSYGYVEKDELTRRYHVGSQVVSLSRSVLTRLPMREAAKPYLRQMMQRTGECAHL
                     AIPAQGKVMYIDQVESPVTLRVNAQVGTMNPLHCTALGKVLLAYGDLDLPSDLAAFTT
                     NTITDPETLHRHLEEIRQLGLAVDDEEFDLGVRCIAVPVFDFRGKAVGAIGISGPATR
                     MTAERMPDLTAIVVEIGKALSERMTFSR"
     gene            6114..7976
                     /locus_tag="PKL11_02475"
     CDS             6114..7976
                     /locus_tag="PKL11_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ12493.1"
                     /translation="MTSSPQISQNELTHQRTAHAAAYLRGALPHRMDLSLSEALVIGL
                     LRQGVKTYFTVLGHGSTELGEVLRVYQSAGLVKVYGLRSEIEASHAATALRWAADEKS
                     AVVTSIGPGALQALAASLAPASDGIGVWYIFGDETTQDEGFNMQQIPKHEQGLFLQLA
                     ATMGRAYSLHTPLALPTALQRGASIVDHPTRPAPFYLLLPMNTQAAWLPDFNLEELPS
                     AGFSTPGAAEGDYPQVAQWIREADRIIIKVGGGGRKSSSELTKLLEHSGAVLVHTPIA
                     TGCVPYSHPQNMGVGGSKGSLCGNYAMDNADLLIAVGTRAVCQSDCSRTGYPQVKHVI
                     NINADLDDATHYTRTLALVGDVRRTLEKLNHVLAETTPQDKSTWLAACAEQKTAWQTF
                     KLQRVLNPVLPDDYWGRSVLTQPAAIKIATDWAKAQGVICFFDAGDVQANGFQIVEDE
                     RPGQTFTETGASYMGFAVSALLATGASSRPFYALALTGDGSFTMNPQILIDGAEHKAH
                     GCILLLDNGRMGAITGLQEDQYGVGFATWNTMHVDYLAWARSVPGVLALDGGNTPASL
                     QAALEQARQHDGLSLIHVPVYFGPDPMGGMGVYGRWNVGNWCENVQSLRHKIGL"
     gene            8048..8890
                     /locus_tag="PKL11_02480"
     CDS             8048..8890
                     /locus_tag="PKL11_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNZ12494.1"
                     /translation="MKIMTSEKITELEEIARQLRIDSLKLIYHRGAGHPGGALSAAEI
                     IAALYFHILNIDPQNPDWDQRDRFILSKGHASAVLYSALARRGFFPFSELDHWGGLDC
                     PHQGHPDRLKTPGVEMTSGILGHGVALGAGLSLAARMKKQTYQTYVLLGDGECQAGIV
                     WEGAVTAAKYRLANLTAIVDNNNVQLDGFVHDVMPLEPLAGKWSACGWHVIEVNGHSV
                     RQVVEALELARELHDQPTVILAHTTKGKGVSFMENQSYWHGNVPNNEQLSLALTELGE
                     VANG"
     gene            8883..9839
                     /locus_tag="PKL11_02485"
     CDS             8883..9839
                     /locus_tag="PKL11_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ12495.1"
                     /translation="MAEISMREAYGRALAELGAVNPQVVALDVDTSLSTLSNFFAQRF
                     PERFFNIGIAEPCMVDVAAGLALGGYMPFINGFASLLALRALEQIRTCVCYARTNVKI
                     AASYAGLSDFKDGATHYAITDLANLRALPGMTVIIPADAAEAAAWVPLVAEYDGPVYL
                     RISRAGAIPVHLPDSPLQIGKGRTLRSGNDLTLVATGAMVGRCALAAEELSTQGIHAR
                     LLEIHTLKPLDSDLLLQAAAETGALVSAEEHSIIGGLGSAVAEVLSEGCPVPFVRVGI
                     RDHFCPTSRNLDQLMDACGLSVADIVEAAKQVLERKSPSKKS"
     gene            9888..10514
                     /locus_tag="PKL11_02490"
     CDS             9888..10514
                     /locus_tag="PKL11_02490"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HNZ12496.1"
                     /translation="MKIAVINEMSAADKNADILKALGGRGHDILNLGNTSSSDTPDLL
                     YIHTGLMSALLLNLKLVDFVIGGCGTGQGYLNAVMQYPGVVCGHLLTPLDAFLFARIN
                     AGNCVSLALNQGYGWAGDVNLRQIFDALFTPETGAGYPPHRADPQRQGRELLSQISQA
                     AHRPMVEILPSLPEEVVKTALTYPPYRTFLEQAAAERQDIQSVVEHVL"
     gene            10554..11930
                     /locus_tag="PKL11_02495"
     CDS             10554..11930
                     /locus_tag="PKL11_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12497.1"
                     /translation="MADTTSKLRTRTKLFYGIGDLGNALVNSAIQFFLMKFYTDAALI
                     LPALAGNALLIGKIWDAVNDPLFGWITDKTKSRFGKRRVFMIFGAIPLGISIALLWFV
                     PKADLISTFIWIAATFILFDTLWTLTNVPYYSLTSELTDDYDERSSLTTYRMVMAVPA
                     YLIGVALTPVIVGLFALQRTGYAFIGILYGIIAAVVLLVSAAGLRERKDLVTAKPETS
                     PLKSLLIAIKNKPFVKLCGIYFIINISFAFIKILMAYYIEYQLLMKAETSLVMGLMLV
                     CVTLSLPFWQWVSRKIDKGPAYGLGMLVGGAAVVLTFFLPHHSTMLIYLIAVLAGFGF
                     SAQWIFPWAMVADVADYDRLETGQQRSGVYYGVWGLATKISEALALAAVGWILTGFGY
                     VPNVEQTPHALLGIRLFFGLIPAACIFISLPLLFKYPITRKSHAEVRAKLEAVDKNTH
                     DDKPVQTL"
     gene            11946..13025
                     /locus_tag="PKL11_02500"
     CDS             11946..13025
                     /locus_tag="PKL11_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ12498.1"
                     /translation="MKPIRVAIVGCGRISDLHQMGYRGREDAKIVAVCDTNKAHAKEK
                     AREWAVDRVYTDYQQVLEDKEVDVVELLTPHHLHCPMTVQAAQAGKHISVQKPMALSA
                     AEADEMIAAANKAGVLLRVYETFVYYAPAMRAKEMIEAGEIGDIRAVRMHVNSGTADT
                     SWNVPLSAWLWRFNEKQCGGGPLVFDHGYHLFSLGYYLGGPVEKVFAWIDHTSVDNTG
                     GLVKIDAPAAIMFQYKSPHCYGMLDIEYTPKMRILSDYYADDDRIEVIGEKGILFINR
                     YTARTVDLPELMLFKDGKTTPIPVEGVEWHDSFIATTHDFIEKLKTGGQPRLDGFTGK
                     AVLQFTLAALQSAATGCEVRPDDVK"
     gene            13478..15706
                     /locus_tag="PKL11_02505"
     CDS             13478..15706
                     /locus_tag="PKL11_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ12499.1"
                     /translation="MGKINVGSITGNSGIINIGSGNVIVPDPSLPALHQLPDPPADFV
                     GRVVELEDLVNAAQSMNILIHGLGGIGKTALGLVLAHQLKVRYPDGQLYLHLRGASNN
                     PVTPAAALEQLIRSFAPVARLPEDVEQLANIYRTLLADKHILVLLDDARDAAQVRALL
                     PPRPALAIVTSRGRFDLAGYHTCDVSALPLEDAVKLICAVWGQTGEVTALAARCGRLP
                     MALRAAGALLRRRPDLTPAGVDALLTDTARRLALADPERDNLTVAAAFQASYDQLTAD
                     LQARWRALGVFPADFDAAAAAAVWGDAADPGETLADLHAAGLVDFVTGVRWRLHDLAR
                     DFTCSLCASAEMDALAYRHAAYYLDVLAEANAWYLKGSEHILAGLVLFDRERMNIEAG
                     QAWAAACSTGPITATLCNAYPDAGAYVLPLRLHPWAQIAWLEAALTAARALGNREYEG
                     FHLGNLGNRYRELGEPHRAIEYHQQRLVIAREIGDRHGEGNALGNLGLAWDDLGEPHR
                     AIDYYEQALVIAREIGDRRSEGQTLGNLGSAWDGLGKLYRAIDYYEQALVIAREIGDR
                     CGEGNALGNLGNAWADLNDSRRAIDYFEQDLVITREIGDRRGEGQDLGNLGNAWFALN
                     NPSRAIEYHEQALVIDREIGDRRGEGQDLGNLGNAWAALNDPCRAIDYYGQALALAQA
                     IGDRQGEANRCWNLGLLLEQQGELTRAIELMQVQVDFLRAIGHPDAEEADAYVQEIRQ
                     RL"
     gene            16050..16625
                     /locus_tag="PKL11_02510"
     CDS             16050..16625
                     /locus_tag="PKL11_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ12500.1"
                     /translation="MAIETYNKILSAAHRLFVKQGYTATSMRQVAEAAGIGKATIYHH
                     FPDKQSIVMALLQHNLTRMEEALRLVRAENHPRRRIQVAAEASVDFLFESVDILQIVR
                     REIPGGRDQMQGGFARFFQEYMALLADAIQRGIEQGIFRPVDPVNSARVLMTMIQGTF
                     AMAYLINERAQTPQQAATDLLDIYFQGLDAR"
     gene            16690..17898
                     /locus_tag="PKL11_02515"
     CDS             16690..17898
                     /locus_tag="PKL11_02515"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12501.1"
                     /translation="MSEKAISARTKFGLVLHSPTFVVLWLSEALSLIGDRLIMVALVT
                     LVYDRTGSASAVGLLMLCKAIPALALGSLAGACVDRWNCKWIMVIANLLQGLLVFCLP
                     LSTGIVIIFVIYLAMSTVNQFFVPARSATIPDLVAQEALLSANSLFAISFVIAMAIGP
                     AIGTWITERVSLNAAFYTDAATFLVPALAVTFLVIPRQCRTAAKPGLSSDLRAGLVFA
                     RSQPAVLAALATCSAAFLVIGTISVTGVVITREILNVDSSKFGVMMSALGIGMLCGAV
                     ATGWLGRRFTYVQLSVAGVVLMALGMTALPWSSSLVMASSFTAAIGLGMIIVQVNGQT
                     LLQTIAPEMRGRLMGISQTLTGSVTFLASALVGLLVEHLNVIIVMGSVGMITLAVAAT
                     VAILYHNTSM"
     gene            17913..20591
                     /locus_tag="PKL11_02520"
     CDS             17913..20591
                     /locus_tag="PKL11_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ12502.1"
                     /translation="MQYIKSLTELNQADLSIAGGKGANLGALIQAGLPVPPGFCVITP
                     AYRAFVEANKLELEIRQIVDATRMDDPAALDASSDAIRACFQQGEMPLALADEICRAY
                     ANLQNPPGLVAVRSSATAEDLPDLSFAGQQDTYLNILGEPALLDAVIRCWASLWTARA
                     IGYRARNQISQDEVALAVVIQQMVQSEAAGVLFTANPLTGKRSETVIDATLGLGEALV
                     SGQVEPDHYVVTGSGEKILHKTLGAKALSIQGQVGGGTVTLHHTAASHQALPDDQILA
                     LAQLGQQAQNFFGGPQDMEWAWSEGRLYVVQSRSVTSLYPLPAGLKDEPVEVLLSFGV
                     WQGMLDPYTPLGQDVFSSLIPGILQLFGRKINAREQRVFLSAGERLYVNMTGLLSNPI
                     GRKVVNLFIGAIDPVSSAILTELLKDARFGVKTSLSLKERFQLLRVVRPLAGNVLFNL
                     LWPARGRARLERQIDSVLTEVQGSCDAAQNLAELIESIDAVAFKMPQQMFPRLVGGIA
                     AGQAPYQNLLLRASQIPGAPELLMEMARGLPHNVTTQMDLALWRTAQVIRADDHAAAH
                     FSSTEVGRLVMEYHSEKLPAAAQIAIRSFLSTYGMRGIGEIDLGRPRWRENPTGLFQV
                     LKSYLQIDPALSPEAVFQRGAGKIWQVQAQLEQIFSEAPKGKRISPHLVRLLLERFRE
                     LGGLRETPKFFVVRLLGIYREALLAAGQKLVEAGRLTAAEDVFFLHLWELKTLGDSET
                     RHWQTLIAERRATNAREARRRRIPRIMLSDGTAFYDAPSTGEAANTLSGAPVSAGVVE
                     GVVRVVFNPHGVQLTPGEILVCPATDPAWTPLFLAAGGLVMEVGGMMTHGSVVAREYG
                     IPAVVGVSQATTRLQTGQRVRVDGGSGVITVLEDQT"
     gene            20757..21737
                     /locus_tag="PKL11_02525"
     CDS             20757..21737
                     /locus_tag="PKL11_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12503.1"
                     /translation="MGKFLQTEKPKQVAFKQISATISNAAKAEGIYKNHAYPFCLPRE
                     LAEENLYPPIRTTIREYFEQNKIKWHDGQNGNPSNHMCDSQVCCANFLFPFADKPQAL
                     AALLKPIFPDLHEMLPIEDGLYVAFEWIGQENYLHEKVPRNGQRTRGANCTSADAAVR
                     FKRADGREQISLIEWKYTESYSRMSLEVAASGQSRVEIYRWLFEQPDCPIDKTRLPGF
                     EALFFEPFYQFMRQQLLAHEMEKAHELGADIVSLLHISPAHNLDFRTITSPRLESRGL
                     SATDVWKTLVTLPGRFISISTESLFGQLDLSCYPELLEWQTYIQARYTWM"
     gene            complement(21941..23704)
                     /locus_tag="PKL11_02530"
     CDS             complement(21941..23704)
                     /locus_tag="PKL11_02530"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNZ12504.1"
                     /translation="MNINQLREEYLKFFEARGHRRIPSAPLLPENDPTVLFTTAGMHP
                     LVPFLLGEPHPLGKRLVDFQKCIRTNDIDEVGDASHLTFFEMMGNWSLGDYFKEESLA
                     WSYEFLTRTLGIQPSSLSVTVFAGDADAPRDDEAAGIWKRLGIPVDRIHYLPKKDNWW
                     GPAGQTGPCGPDSEIFYDTGRPDHPGCGPGCSCGKWFEIWNNVFMEYDKKADGNFTRL
                     KQQNVDTGLGLERTHAVLQGLDDIFKIETLWSLVQKIEELSGRSYAENPSPFRVIADH
                     LRAASFAIADGARPSNVESGYVVRRMIRRSVRFGRELGIHDNFLGALSCTVVEMMQAT
                     YPELERQREVIAGALDAEETKFKHTLERGLRQVNKVFDHLKENHINLVSGEDAFDLFE
                     TYGFPLELTQELARENGLPVDEEGFQRCYAEHRESSRQNLDQKFKGGLADHAEKTTSL
                     HTATHLLHKALRTVLGTHVQQKGSNITPERLRFDFSHPERVTPEQLAEVECIVNDQIA
                     ADLPVNMEIQTLQQAMESGALAFFSEKYGEQVKVYSIGDFSKEVCGGPHVTRTGAMGH
                     FRITKEEAVGQGIRRIRAVLE"
     gene            complement(25219..26796)
                     /locus_tag="PKL11_02535"
     CDS             complement(25219..26796)
                     /locus_tag="PKL11_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNZ12505.1"
                     /translation="MMKPHNILITVLLTITLAACSTPAVTTTSSNPAVEVTTTSSPVG
                     TSAPVVVTFADPVLEAMIRGAMGKTEGDITLAEAQAVTRMNFSNEIQSYISEETPIKD
                     LSGLENFTSLETLDLSSHAVTDISPLQELTKLTTLSLAGNPVADVSPLAGLTKLKVLM
                     LSGSKAQDYSVLSNLVNLQVLLLDNSTISDLTPLAGLTNLRQLHLANSSIEDLYPLET
                     IYPNLEDKDFIIATTLAELGFNLDVNSHQAVYDSESASFTIHHAAWGQPQEEWHTNTI
                     RTSMYTGSGHKVLLGYYGDLDAYVFMMHKEGEPPVNYVYDAHSGDINISEEDRASIEQ
                     VVRAAFEVMEDEDPLLAPMRVFHAIVKDTFDMTAAKLYALPYAPPSLKSFGFFADEPN
                     AVYLYEYRGEKDVNMEVHRPEWGEKEFDVRFFTPISDEYRIVVTWHAAEKKFIASVDD
                     NSQGGGSFEYFIDTGEHQDIWCSYPDKTVEEYFIKAYNDPAIVDVYQHSVDLMEQYVS
                     DTFGMTIEELYALPTGE"
     gene            complement(27105..27392)
                     /locus_tag="PKL11_02540"
     CDS             complement(27105..27392)
                     /locus_tag="PKL11_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ12506.1"
                     /translation="MEGSKLLKFEDWESEKIKASDFLSLAEELEPGYQVARLRMFRGL
                     TQAQLAEMVGTRQPSIARLENGTSAPSFSFLSKIVEALGAKIEFKLIPDTK"
     gene            complement(27958..28287)
                     /locus_tag="PKL11_02545"
     CDS             complement(27958..28287)
                     /locus_tag="PKL11_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12507.1"
                     /translation="MFLIKGNHDRLSRSFYEAHGVTLIINSSQVEVVSQIKLIFSHRP
                     IVQLEDGWINFHGHIHNVPPPPEGSNLGPDHINMSVEVREYRPWRLSDILKTSKDHPT
                     ENDVSLR"
     gene            complement(28331..29749)
                     /locus_tag="PKL11_02550"
     CDS             complement(28331..29749)
                     /locus_tag="PKL11_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12508.1"
                     /translation="MNNQSMEPIIKCQYFEEPLLQFADGREHIDPKIGISRYGPKSFS
                     PKRKHPDTVRIGFIGSADTIDSAKSWLKKTSEGVLCDEDHVEFPGYMEDRGFFSRLIF
                     DQDWNGQLTQKEIDKILEKKPKERFEFLLEILEEKLDLLSKKDQPPQYIIVALPDSIY
                     GKCKGVDYRDQEKGLVHRDLRRAFKALAMKYQIPTQFLRQQTIDGREKDDPSKVAWNF
                     FTGLYFKAGGIPWGPIGLTPGTCYVGIAFYRPLGSKFNSMQTSLVQAFDEHGDGLILR
                     GPEFQWNSYRMNSRSPHLNDRDASNLINIVLDRYEKEMGQTPQRVVIHKSSTFWPGEK
                     DGFKQALGQRVKKFDLMSFDTRQSHIRLLTTSSYPPLRGTRFMVGDMDFLYTTGFISE
                     LGQSHVLHVPSPILITDHVGQDTPRETLLKEVLIMTKMNWNSSRLGGKAPITLRFSDI
                     VGEIMREIPSDREPMPQFKFYM"
     gene            complement(29742..31052)
                     /locus_tag="PKL11_02555"
     CDS             complement(29742..31052)
                     /locus_tag="PKL11_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNZ12509.1"
                     /translation="MTISPTDLKALLQKSGNRCAFPGCPSMLTVQESDDSTVILSNVA
                     HIVAQREDGPRGKFSLPLNRRDEEGNLMLLCPEHHKIIDNKPQLYTVERLWGMKEDHE
                     KLVQTALGNAIDKTTKRDQFVDKYPIESVYSSLLEVIKTPVYIYQSTPTKDAIANYAI
                     EQLPLFTQPDIHLPYILKEDRLYTFQDPRNADSPFHTVVELNSVRQIASRKWWDDPVK
                     SRWFVELLNITLQIFTSQKGLVYDPDHYRYYFVPDQPGQPKDIEYKPLNQNVAKKHVV
                     WQPITKKTGLPKSYWLHRSISLRSYYVSSGRWCLALRPEFRVTKDGNLPLDSEKIGAK
                     VTRKKSKMFNYDLLGEINFWRSFLGNNEPRIVIDLGSSSLVISTTLLSGDINWPGIPE
                     KYARTFTNVEYAEDLFTWAKLQNVLENEELFDDLDEGVEDDIDE"
     gene            complement(31191..34157)
                     /locus_tag="PKL11_02560"
     CDS             complement(31191..34157)
                     /locus_tag="PKL11_02560"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNZ12510.1"
                     /translation="MMFNESNSVELFIRDLLCGKTGKQIGEQPGLYAGALAQLARGQG
                     WVYKPADKLHRSTQDVLLEDELRAALIRLNPEIAEQPERADEVLYRLRAVLLSVKSDG
                     LVRANEEFTTWLRGERSMPFGPNHEHTSVRLIDFENIGNNQCVLTNQVIFQAGSQTRR
                     FDLVMFVNGIPLVIGEAKTPTRPAISWVDGAVQVQDDYEVNVPAFFAPNVFSFATEGK
                     TYRYGAIRQPLESWAPWREEDNNEYSGLNELQTAALGMLRPAIILDILKNFTLFATDK
                     KHRKTKVICRFQQYHAANQIAERVVLGRVKKGLIWHFQGSGKSLLMVFAAQKLRMHTA
                     LKNPTVLIVVDRIDLDTQITATFNAADVPNLISTDSREELRTLLAQDVRKVIITTIHK
                     FGEADGVLNNRDNIIALVDEAHRTQEGDLGRKMREALPNAFLFGMTGTPINQRDRNTF
                     FAFGAEEDEKGYLSRYSFEESIRDQATLPLRFESRLVELRVDKQSIDEAYANLTGHLS
                     EADQANLARQAARMAVLVKAPERVQRIVSDIVDHYQEKVEPNGFKAMVVTFDRQACVL
                     YKKAMDEILRPEASEIVMSVFHNETEYKAYDRSKEDEERLLDRFRDPADPLRFLIVTS
                     RLLTGFDAPILQVMYLDKPMKEHNLLQGICRVNRPYPGKDYGLIVDYIGVFDDVARTL
                     AFDEANMQQVITNLKALHAQLPEAMQSCLAYFPNVDRALGGWEGLVAAQACLPDNETR
                     DKFAGDFSTLSQLWEAISPDPVLSDYEDDYRWLGQVYESVKPPSGSGKLLWHALGAKT
                     LDLIHENVHVERVRDDLDTLVMDADFLDELLGERDPEKIREVEIKVIARIRKHGNNPK
                     FIALGKQLEDLKNRMEQGLVNSLDYLKQLLEIARKVLQAEKDVDPEEERQSAKAALTE
                     LFNETKTAQTPVIVERIVADIDEIVRNVRFPGWQQTIAGEREVKQALRRVLLKYQLHK
                     EQELFDKAYGYIKEYY"
     gene            complement(34154..>34953)
                     /locus_tag="PKL11_02565"
     CDS             complement(34154..>34953)
                     /locus_tag="PKL11_02565"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNZ12511.1"
                     /translation="SIKNSKGSVNPYINWSDLARYKFPLPPLDEQRRIAEILWAADEV
                     ITKNLVTLQRIISLKDSVEEESFFRCRDSGQPTLLSEIAEVNPSSKPKCKDEEPVSFI
                     TMADVSEDGEIINQQSKMYGEVKKGYTVFQDGDILFAKITPCMENGKGALALNLINGI
                     GFGSTEFHVLRPMDSRDTAFIYFLTMNKKFRQQARRFMRGTAGQLRVPKDFLDEYVLD
                     CLPEVTLREEMGRKLIEVNSSYLEQKRSIEKAKIVKAELLQKLLGGQ"
BASE COUNT         8012 a         9309 c         8999 g         8633 t
ORIGIN      
        1 tctccatctg atttcaaagc tacgccatga tgctgccttg tatatgccct acttaggccc
       61 caagcccaaa cgtggtccga cgcccaggct gggtgaaaaa gtcaatgtgc gccagcttcc
      121 agacacctat ctcaaagaaa ccgagattaa agatgggttt gagacgcgca cctatcaatt
      181 accgctgctt ctcagtgagt ttgcccaacc tttgaatatc gtcatcctgc tgagaaccaa
      241 tttgacgacg aaagcttgtt cgcatgtgat cctgttcagt agcgatctgg agctggctca
      301 tgacaaactg attgactttt atagcctgag attccaaatc gagtttaact ttagggatgc
      361 gaaacaattt tggggattgg aagacttcat gaatgtttcc gaagttgcag tgacgaatgc
      421 agccaatctt tcgctgttca tggtcgatat atctcaagtg ttaatgtgcg agtaccgaca
      481 aaatgatcct gatttcagta tcctggattt gaaagctcac taccgaggtt accgctatgt
      541 gtccgaaaca ataaaaatgc ttccgcaaat gcccgatagc aatttagttt tgcagctttt
      601 ttctaaagtg gccgctttag gccgtattca cccggcaaaa ctgccggttt catcaccata
      661 attggcgaag gtattgggta taatcaacca tcaaaatttc cccaggggct gacgtgcatc
      721 agcccaaatt tttttaataa ggagaactgg atgaaattcc gccccttcct ggcagttgcc
      781 atgttggcaa ttctgcttac ttcatttctc accgcctggt cgccggctgc acctgtagcg
      841 ctggtgtccg aaaaggtggc cccccttctg ctgcagcgca ttgcggctga aggccatacg
      901 gaatactacg tcatcatggc ccaacaggcc gatgtttcgg ccgcggccaa cctgcccacc
      961 aagctggaga agaccaccta cgtcttcaac gcgctgaccg ccatggccga aaaaacccag
     1021 aagcccctcc tgacgcaact ggaaaaagaa ggcgtgtctt accagtccat gtacattcag
     1081 aacatgatca aagtggacag cggcatcacc accctcacct ggctctcggc tcggcccgag
     1141 gtggcgaaga ttgttgtgcc ccccaccccc gtcatggacc ccgtcatcaa agaaatgacg
     1201 cctgaacaga agaccaaggc cattgaatgg aacatcatcc gcattggcgc agatgatgtc
     1261 tggggcatgg gctttaccgg caatggactg gttgttgcct ccaacgatac cggcgtggaa
     1321 tatacccacc cggcgttggt caatcactac cgcggcacta ccggccccgg cacctatgat
     1381 cacaactttg actggtggga tggcaccacc ggttacgatt acccgcacga ctacgatggg
     1441 cacggtacgc ataccactgg caccatggtc ggtgatgacg gtggcactaa ccagattggt
     1501 gtggctcccg gcgcacagtg gattgcctgc cccggcattg gcggcggcgg caccgacacg
     1561 gtggaatgct tcgagttctt cctgtctccc tgggatctga accgtgccaa cgctaatccc
     1621 gctctggccc cggatgccat taacaactcc tggtacgacc cctctggtta cgattaccgc
     1681 accatcattc agaacctgaa tgatgctggc gttgccgtga tcaaatcggc aggcaaccag
     1741 ggctcagcct gcaacagcat ctccaatcct ggctatgtgc ctgagatcat ttccacggcc
     1801 aactttgccc agggcgatgt catctccagc agttccagcc gcggccccag cagctactat
     1861 ggcgaaacca tcctcaaacc tgaggtggca gcccccggca ccaacatccg ttcatcggtt
     1921 cctggcggcg gatatgaagg cggctggtcg ggcaccagca tggcggcccc gcacaccacc
     1981 gccctgattg cgctgatgtg gcaggccgcg ccctgcatca tgggcgatgt gcccaccacc
     2041 aagacgatcc tgcaggatac tgctgatgaa aagattgacg cccaatgcac tccgtttgta
     2101 ggccacccca atgatgtctg gggctatggc atcctcgatt cagtcgaagc ggtcaatgca
     2161 gccatcgcct tctgcggcgg cctgggtacg ctggatggta ctgtgaccga tgccagcacc
     2221 accaacccac tggaaggtgt cacggtcaat gctgtggaaa ccggtggctt caaccgtacg
     2281 attgccacca acgccagcgg ctactacacg gctgaactta ttgaaggttc ctacactgtc
     2341 accgccagct tgtatggcta tgaaaccgcc accattaccg atgtggccgt aactgcagat
     2401 acaaccacaa ctcagaatat tgccttgacc ccggcagaaa tcttcactct cagcggcgta
     2461 gtcaccgatg ccgtgagcac tgccccgctg caggccgcca tcaccatttt ggatacccct
     2521 ctggcgcccg ttaccagcga tcccggcacc ggtgcttaca gcatcgacct gcccgccggc
     2581 acctacatgc tgcgcgccga agccgatgcg cacctgacac tggaaatgga gatcaccatt
     2641 tcggcagacc tgacccagaa cctggcgctc gaacccgtgc cctgcattct gctggtggac
     2701 gacgatgccg gcaacagcta cgaaacctac tacactgctg ccctggacgc cttgggactg
     2761 gattacgctc tctggagtac atcaggtggc tcaccaaccc tggcagacat ggtcgattac
     2821 accatggtgc tctggttcac cggcaatgac tacagcacaa caatctccag taccgaagaa
     2881 gctgacctgg ctgcctatct gaaccagggc ggcaacttct tcctgtcttc gcaggattac
     2941 ctttacgatg ttggcggttc aaccaccttt gatacgacct atttgcagat tcaaagcttt
     3001 accagcgatg tgaacaagaa caatcccgtt ggcgtggccg gcgacccgat tggtgatggc
     3061 ctgggggcct acaccctgac cgctcccagc ggctttggta cgctctacac cgataacgtg
     3121 gtaaagactg caaccgcagg tgttaccaac cccttcaagt ggaacgatat cgccaacttc
     3181 aacagcacca gttacgacag cggcaccttc aagactgtct tcctggcctg gccaattgag
     3241 gggttgtcgg cgctggctga ccgccaggca gtgttgggcg ctgtggtaga tttcttcggc
     3301 ggctgcacca cctgcgaacc gatcaccaat gcagatttca cctggctgcc aattgacccg
     3361 gcggctggcg aagctgtagc ctttacgagc acaatcgcta ccggcgatgg caccatcacc
     3421 tatagctggg acttcggcga tggcagcacc agtgacgtgc agaatcccac ccataccttt
     3481 gccgatggcg gcgactttac ggttgttttt atcgcatcca acacttgctc taacagcaac
     3541 cgcaccgaga tcgtcagtgt catcccgttt gtaaaaatct tcctgccgac cattctccgc
     3601 taatcctggc gaagcggcta tgtgatacaa caacagggtg cgcacaagcg caccctgttg
     3661 ttatctgagt aattattgtt ctggttacag ccgggatcca gttctttcca gactcaattt
     3721 gctgcatcca attgtgataa tcaaaacatc cgactccagg cttttggcca gcgctcgacg
     3781 acaattttct tttgtgtgaa aaattcaact gcatcgttgc cctgtccatg caagtcgcca
     3841 aagaagctgt ccttccaacc gctgaatgga aagtacgcca tgggggcagc cacaccaata
     3901 ttaatcccaa tattgccggc ctgcacctcg taacggaatt tgcgtgcggc tgatccactc
     3961 gaagtgaaca gactggcctg attgccgtac gtaccgcgat tgatgacagc aatcgcctca
     4021 tcaaaactat ccatatgtgc aacccccagc accggcccga agatctctgt gcgggcaacc
     4081 tcacccccaa gcggaagatc caggaggatg gttggccgta caaaataacc gttttcgtaa
     4141 ccctgtatga ccgcccggcg tccatcaact ggcaggctgg cgccttccgc agctccctgg
     4201 ctgattaaac gctcaatgcg ttccttgctg gctgaattga tcacgggtcc catctggatc
     4261 cccggttcaa ggccattgcc taccctgcgg ttggcagccg cttcgcagat tctttcgaca
     4321 aatggacgac gcgcttcgcc aatggttata gccagcgaag tggccaggca gcgctgacct
     4381 gcacatccaa aagctgaatc agcagtaatc ttaaccgcca tgtccatgtc tgcatctggc
     4441 aggatgataa tcggattttt cgccccgccc tgggcttgca ctcgcttgcc gttctcagct
     4501 gcccgccgat aaatatagcg ggcggtctcg gtggctccca caaaggtaat cgcccgaatc
     4561 tgtgggtggt caagaatact gtccacggct tcctttgcgc cattaaccag attgaccacg
     4621 ccctttggta atccggcttg ctcaatcaat gtaaagatca actgcatggt cattggcgtt
     4681 ttttcagacg gtttgagcac gtaagtattg ccacaggcca gggcatacgg cagataccaa
     4741 aacgggatca tccccggaaa gttaaaggga gcaatcgtgg cacacacgcc aaccggctga
     4801 cgcagcataa tctcgtccaa acccggcgcg atatcttcgg caatttcgcc tttcatcatc
     4861 atcggaatac cacaggctac ttctacgttt tcaattgcgc gtcgcatttc cccgatggct
     4921 tcttcgactg tcttgccgca ttccatcgtg atcgtccgcc caatttcttc caggtttgac
     4981 tcgagcagag ccttcagctt gaacaggtat tggatccggt cctgcgctgg cgtctggcgc
     5041 caggcgggca gggcggccgc tgcagcctgg gccgcggcat ccacttctgc agcagtcgaa
     5101 agcggcgtgc gccacagcac ctcacccgtg gccggattga tcacatctac ggtttcagca
     5161 gctttcgatg cgacccaggt cccatcgata taattacgga acgttttcac aggaaacctc
     5221 accttattct ggaatatgaa atagattatt gcttataata caacaatatt gtataatatg
     5281 caataatgac cgaaatacaa tcccttgccc gtggtttgaa aatactatcg ctcctgagcc
     5341 aatctccaga tggcatcagc attactgaac tggccgagta tcttgacgtt gacaaaggga
     5401 gtgcttcgcg cctggtttcc acactgtcga gttatggcta tgtcgagaaa gacgagctta
     5461 cccgccgcta tcatgtcggt tcacaggtgg ttagcttgag ccgtagtgtg ttaacccgtt
     5521 tgcccatgcg tgaagctgcc aagccatatc tgcgtcagat gatgcagcgt accggcgagt
     5581 gcgctcatct ggccattccc gcccagggaa aggtgatgta tattgatcag gttgagtcgc
     5641 cggttacctt gcgcgtcaat gcccaggtgg gaaccatgaa ccccctgcac tgcaccgcac
     5701 ttggcaaggt tttgctggct tatggtgatc tggatctgcc ctctgatctt gctgccttta
     5761 ccaccaatac cattacagac ccggaaacat tgcaccgtca tttagaagaa atcaggcagt
     5821 tgggccttgc tgtagacgac gaagaattcg atctcggtgt acgctgcatt gctgtgcccg
     5881 tttttgactt ccgaggtaag gctgtaggag caattggcat ttcagggcct gccacacgga
     5941 tgacggccga gcgaatgcca gatttaaccg caatcgttgt cgaaattggc aaagcccttt
     6001 cagaacgcat gaccttctct cgttagaaaa cgcccgcaat ttgattcgcg ggcgtttttt
     6061 attgacctta tagaacaacc atattgcata atacgcaaca actggaagca gatatgactt
     6121 catcccccca aatatctcaa aatgaattaa cccatcagcg caccgcacat gcggctgcat
     6181 acttgcgcgg cgccctgcct caccgcatgg atctctctct ctcagaagcg ctggtgatag
     6241 gcttgttacg tcagggcgtt aagacatatt tcaccgtact cggacatggg tccaccgaat
     6301 tgggtgaggt gctgcgagtc tatcagtctg ccggcctggt caaagtttat gggttacgca
     6361 gtgagattga agcttcgcac gcggccaccg cgctgcggtg ggccgctgac gaaaagtcgg
     6421 ctgtggtcac atccattggg ccgggcgctc tacaggcact ggctgcttct ctggcccccg
     6481 cgtcagacgg cattggtgtc tggtatattt ttggcgacga gacaacccag gacgagggtt
     6541 ttaatatgca gcagatcccg aagcatgagc agggcttgtt cttgcagttg gctgctacaa
     6601 tgggccgcgc ttacagcctg cacaccccat tggcgctgcc cactgccctg cagcgtggcg
     6661 cgtcaattgt agatcacccc acccggccag ctccattcta cctgctgctt ccaatgaata
     6721 cccaggctgc ctggctgcca gattttaacc tggaggaact acccagcgcc ggattttcta
     6781 cccctggcgc ggctgaagga gattatccac aggtcgccca atggatccga gaagcagatc
     6841 gtatcatcat caaggttggc ggtggtggac gcaaatccag ctctgaactg accaaactgc
     6901 tggaacattc tggtgctgtt ctggttcaca caccaattgc caccggctgc gtcccataca
     6961 gccatccgca aaacatgggc gtcggcggtt ccaagggctc attatgcgga aactatgcca
     7021 tggataatgc tgatctcctg attgcagttg gtacacgtgc cgtctgccag tcagactgct
     7081 cacgaacagg gtatccccaa gtcaaacatg tgatcaatat caatgccgac ctggatgatg
     7141 ccacccacta caccagaacc ctggcgttgg ttggagacgt acgcaggacc ctcgaaaagc
     7201 tgaaccatgt tcttgctgaa acaactccgc aggataagtc aacctggttg gctgcctgtg
     7261 cagagcagaa aactgcctgg caaaccttca aacttcaacg tgttttgaat ccagtcttgc
     7321 ccgatgatta ttggggcagg tcagtgctaa cgcagcccgc agcaatcaag attgccacag
     7381 attgggccaa agcacagggc gttatctgct tctttgatgc gggagatgtc caggcaaatg
     7441 gttttcagat tgtggaagat gaacgtccag ggcagacatt caccgagact ggtgcctcct
     7501 acatggggtt cgccgtatcg gcattactgg ccacgggtgc gtcatccagg ccgttctatg
     7561 ccctggccct cactggcgat ggatccttca caatgaatcc acaaattctg atcgacggtg
     7621 cagaacacaa ggcccatggc tgcatcttgc tgctggataa tggccggatg ggtgccatca
     7681 ccgggctgca ggaagaccag tacggcgttg gctttgccac ctggaacaca atgcacgtgg
     7741 actacctggc ctgggctcgg tcggttcccg gcgttctggc gttggatggc ggcaataccc
     7801 ccgcctccct gcaggccgcg cttgaacagg cccgacagca tgacggactc tcgctaattc
     7861 acgttccggt ctattttggg ccagatccca tgggtgggat gggcgtttat ggacgctgga
     7921 atgtaggaaa ctggtgcgag aacgtgcaat cattacgtca taagatcggt ttatgacacg
     7981 gatcagaccc acacaaaagg cgataaccat tttagtttag ttgccgttca agaaatttga
     8041 ggtttttatg aaaataatga catccgaaaa aataaccgaa ttggaagaga ttgctcgcca
     8101 attgcgcata gatagcctca agctcatcta ccaccgcggc gccgggcatc cgggcggagc
     8161 gctctcggcc gctgaaatca tagccgcgct ctatttccat attctgaaca ttgacccgca
     8221 aaatccagac tgggatcaac gcgaccggtt cattctgagc aagggccacg catcggctgt
     8281 gctctactct gcgctggctc ggcgtggctt ctttccattc tctgaacttg atcattgggg
     8341 cgggttagac tgcccacacc aggggcatcc tgaccgcttg aagacccccg gagtggagat
     8401 gaccagcggc attctcggtc atggtgtggc gctgggcgcc gggctttccc tggctgctcg
     8461 aatgaaaaaa caaacctatc aaacctacgt cttgctcggg gatggtgaat gtcaggccgg
     8521 tattgtctgg gaaggcgctg taacggctgc caagtatcgt cttgcaaacc tgacagccat
     8581 cgtggacaac aacaacgtcc agcttgacgg atttgttcat gatgtgatgc cccttgaacc
     8641 tttggctggc aagtggtctg cttgcggctg gcatgtgatc gaggtgaatg ggcattcggt
     8701 caggcaggtt gtagaggctt tggaactggc aagggaactc catgaccagc caaccgtcat
     8761 cctggcacat accaccaaag ggaaaggggt ctcgtttatg gaaaaccaat cctattggca
     8821 tggcaatgtc ccaaacaacg aacaactctc gctggctctg actgaacttg gggaggtggc
     8881 aaatggctga aatttcgatg cgcgaggctt atggtcgggc gctggctgaa cttggtgccg
     8941 tcaatccaca ggtagtggct ctggacgtag acacttcttt gtcgacactt tcaaactttt
     9001 ttgcgcagcg ttttccagag cgattcttca atattggcat tgccgaaccc tgcatggtag
     9061 atgtggctgc cggactggca ttgggcggct atatgccgtt tatcaatggg tttgcttcac
     9121 ttctagcgct tcgcgccctg gaacagattc gcacctgcgt ttgctatgcc cgcaccaacg
     9181 tcaaaattgc cgccagttat gccggcctgt cagacttcaa agatggcgcc acgcattatg
     9241 cgattaccga tcttgccaat ctgcgtgcct tgccggggat gaccgtcatc atccccgcgg
     9301 atgcagccga ggcggccgcg tgggtgcccc tggtggcaga atatgatggc ccagtttatt
     9361 tacgcatcag tcgggctggg gcaattcctg ttcatctacc cgatagcccc cttcagattg
     9421 ggaaggggcg caccctgcgc tcaggcaatg atctgactct ggtggctacc ggcgcaatgg
     9481 tcgggcggtg tgcgcttgcc gcagaagaat tatcgacaca gggcatccat gcccgcctgt
     9541 tggaaattca caccctcaaa ccgctcgaca gcgacctgct gctccaggct gctgcagaga
     9601 ccggcgcgct ggtctctgct gaagagcaca gcatcatcgg cggtttgggg agcgcggtgg
     9661 ctgaagtgct gtctgaaggt tgcccggtcc catttgtcag ggttggcatc cgcgatcatt
     9721 tttgtccgac cagtcgcaac ctggatcagt tgatggatgc gtgcgggctg tctgtggcag
     9781 atattgttga ggctgcaaag caagtcctgg aaagaaaatc gccttcaaag aaatcctgaa
     9841 cacacgtttg acaattgggc ctaaaggaaa attccaaagg agaatccatg aagattgccg
     9901 tcataaacga gatgagtgct gcagataaga acgctgatat cctaaaagcg ctcggaggtc
     9961 gcgggcacga tattcttaac ctgggcaata ccagcagcag cgatacacct gacctgttat
    10021 acatccatac cgggctcatg tcggctctcc tgctgaatct caagctggtt gactttgtaa
    10081 tcggtggatg cggcactgga cagggctatt tgaatgccgt aatgcaatat ccgggtgtgg
    10141 tttgcggtca cctgctcacc ccgctcgatg ccttcctctt tgcccggatt aacgccggta
    10201 attgcgtttc gctggctttg aatcagggct acggttgggc tggagatgtg aacctgcgcc
    10261 agatatttga tgccctgttc acgccagaaa cgggtgcggg ctatccccct caccgggcag
    10321 atccccagcg tcagggtcgc gagcttttat ctcaaatttc acaggcagct caccgcccaa
    10381 tggttgagat tctgccttcc ttgccagagg aagttgtcaa aaccgccctg acatacccgc
    10441 cctaccgaac gttcttggaa caggcagccg cagagcgtca agacattcaa tctgttgtag
    10501 agcatgtctt ataaaacggc caacaagttc attcagtgag aaggaaaatt aaagtggctg
    10561 atactaccag taaattgcgt actcgcacca aattgttcta tgggatcggc gatctcggta
    10621 atgctttggt caactcagca attcagttct tcctgatgaa gttctacacc gatgccgcgc
    10681 ttattttgcc agcgctggca ggaaatgcct tactcatcgg caagatctgg gatgcagtca
    10741 acgacccact tttcggttgg atcaccgaca aaaccaaatc acgctttggc aaacggcgtg
    10801 tgttcatgat ctttggagcc attccacttg gcatctccat tgcactgctc tggtttgttc
    10861 ccaaagctga cctgatatcc acctttatct ggattgcagc aacctttatc ctgtttgaca
    10921 ccctttggac ccttaccaat gtgccgtatt actccttgac ctcggagcta accgacgact
    10981 acgatgaacg ctccagcctg acgacttacc gcatggtcat ggcagtgccc gcctatctca
    11041 tcggcgtagc cttgaccccc gtcattgtcg gcctgtttgc cctccaacgc acaggttatg
    11101 cgtttattgg catcctgtat ggcatcatcg ctgctgtagt actgcttgtt tcagccgcag
    11161 ggctgcgtga acgcaaagac ctggtgaccg ccaaacccga gacttcaccg cttaagtcac
    11221 tcttgattgc cattaaaaac aaaccatttg taaagttgtg cgggatctat ttcattatca
    11281 acatctcatt tgctttcatc aaaatcctga tggcctatta catcgaatat caactattga
    11341 tgaaggcaga gacttccctg gtaatgggtt tgatgttggt atgcgtgacg ctctcgttgc
    11401 cattttggca gtgggttagc cgcaaaatag acaaaggccc cgcttacggt ttgggaatgc
    11461 tggtcggcgg tgcagccgta gtgctcacat ttttcctgcc ccaccactcc accatgttga
    11521 tttatctaat tgccgtgctg gctggctttg gattttcagc ccaatggatt ttcccctggg
    11581 ccatggtagc agatgttgct gattatgatc gtcttgagac gggccaacag cgcagcggtg
    11641 tatattacgg cgtctggggc ctggcgacaa aaatttctga ggcgctggcg ttggcagcgg
    11701 ttggatggat cttaactggg tttggttatg ttcctaacgt agagcagacc ccccacgcac
    11761 tgcttggcat ccgtttattc tttggactca tacctgctgc ctgcatcttc atctccttac
    11821 cgctattgtt caaatatcca attactcgta aaagccatgc tgaggtaaga gcaaaattgg
    11881 aagctgttga taaaaacact cacgacgaca aacctgtcca aaccctgtaa ttgtggagat
    11941 aaagaatgaa accaattcga gttgctattg ttggttgtgg ccgtatttct gatctacatc
    12001 aaatgggata ccgggggcgc gaggatgcaa aaattgtagc cgtatgcgac accaacaaag
    12061 ctcatgctaa agaaaaagcc agggaatggg cggttgaccg ggtctataca gactaccagc
    12121 aggtccttga agacaaagaa gtggacgtgg tagaactcct cacacctcac cacctgcact
    12181 gcccaatgac ggttcaagct gctcaggccg ggaaacatat ttcagtgcag aaacccatgg
    12241 cgttatctgc ggctgaagca gatgaaatga ttgccgcggc caacaaggct ggtgtgttgc
    12301 tgcgggttta tgaaacgttt gtttattatg ccccggcaat gcgagcaaaa gaaatgatcg
    12361 aagccggtga aattggcgat atccgtgctg tgcgtatgca tgtcaatagc ggcacagccg
    12421 acacatcctg gaatgtgccc ctttcagcct ggctgtggcg ctttaacgaa aaacaatgcg
    12481 gtggaggccc cctcgtcttt gatcacggct accacctctt ttcgctcggc tattatctgg
    12541 gggggccagt ggagaaggtc tttgcctgga ttgatcatac ctctgtggat aacacgggcg
    12601 ggcttgtcaa aattgacgct ccggctgcta ttatgtttca atataaatca ccgcattgtt
    12661 atggcatgct ggatattgaa tacaccccca aaatgcgcat cctgtcagat tactacgcag
    12721 atgacgaccg cattgaggtt attggcgaaa agggcatcct gttcataaat cgttacacgg
    12781 ccagaaccgt tgacctgccc gaattgatgc tttttaaaga tggaaaaacg acgccaatcc
    12841 cggttgaagg cgtggaatgg cacgacagtt ttattgccac cacgcacgat tttattgaaa
    12901 agttgaaaac cggcggtcaa cctcggcttg atggtttcac cggcaaagct gtgctgcaat
    12961 tcaccctggc cgcgctccag tctgccgcca cagggtgcga agttcgtcct gatgatgtga
    13021 aataaacccg gttgagataa ccctctatag ggtgacaaat gctgttcaat gcagcccccg
    13081 ggctgcattg aacagcagca agggggagca gtatgcactc gcaacccggc ccagtttttg
    13141 tcacgctgcc agttcggtca gcgctgcctg cccactctgc agccgccagc gcagcggatg
    13201 tgcgcccgcc gaatgatgtt ggcacaatgc gtcatatagt gcaggtcaca ggtgacgcgt
    13261 gagcaggcta aatcaatcaa atcctcaggg cgatacaacc ataacttgac cagataatcc
    13321 tcgccgcctg ttattatacg ctgatttaaa tggcatcatg ccaggcgtct ttgggtgacg
    13381 ttacccgaga agcaggcaga atgaaactga atacccgtct ttgagccctt aaaagctgat
    13441 gtgtaatgac ccggctcttg tatgtataat gaatattatg ggcaagatca atgttggcag
    13501 cattacagga aatagtggca tcatcaacat tggctcgggc aatgttattg ttcccgatcc
    13561 tagcttgccc gcgctgcacc agctccctga tccgccggca gactttgtgg ggcgggtggt
    13621 agaactggag gatctggtta atgctgccca gagcatgaat atccttattc atggattggg
    13681 cggcatcggc aagaccgccc tcggtctggt gctggcccac cagttgaagg tccgctaccc
    13741 cgacgggcag ctttacctcc atttgcgcgg ggcttccaac aaccccgtga cgcccgccgc
    13801 ggcgctggag cagttgatcc gctcctttgc acccgtggcc cgcctgcccg aagatgtgga
    13861 gcagcttgcc aacatctacc gcaccttgct ggcggataag catatcctgg tcttgctgga
    13921 tgacgcccgc gatgcggccc aggtgcgcgc cctgctgccg ccccgcccgg cgctggcgat
    13981 tgtgaccagc cgcgggcgct ttgacctggc aggctaccac acttgcgacg tctcggcgct
    14041 gccgctggag gatgccgtca agttgatctg cgccgtctgg ggccagactg gcgaggtgac
    14101 cgccctggcc gcccgctgcg gccgcctgcc gatggcctta cgcgctgccg gagcactgct
    14161 gcgccgtcgc cccgacctga cgcctgccgg ggtggacgcc ctgctgaccg ataccgcccg
    14221 ccgcctggcg ctggccgacc ccgagcgcga taacctgacc gtggccgcgg ccttccaggc
    14281 cagttatgac cagctcaccg ccgacttgca ggcccgttgg cgcgccctgg gcgtcttccc
    14341 cgccgatttt gacgccgcag ccgccgcggc ggtgtggggg gatgctgccg accccggcga
    14401 gacattggca gacctgcatg ctgccgggct ggtggatttt gtgaccggtg tccgttggcg
    14461 gctgcacgac ttggcccgcg acttcacctg ctctctctgc gcctccgccg aaatggatgc
    14521 cctggcttac cgccacgcgg cgtattatct ggatgtgctg gcagaagcca atgcctggta
    14581 tctgaaaggc agtgaacata tcctcgccgg actggtgctg tttgaccgag aacgcatgaa
    14641 tatcgaagcc gggcaggctt gggcggctgc ctgctccacc ggcccaatca ccgcaacgct
    14701 ctgcaatgcc taccccgatg ccggggccta tgtgctgccc ctgcgcctgc acccgtgggc
    14761 gcagatcgcc tggctggaag ccgccctgac cgccgcccga gcgctgggta accgcgagta
    14821 cgaagggttc catctcggca acctcgggaa tagataccga gaacttggcg aaccacaccg
    14881 ggcaattgag tatcatcagc agcgattagt gattgcccgt gagatcggcg accggcacgg
    14941 cgaagggaac gccctcggca atctgggact ggcctgggac gacctcggcg agccgcaccg
    15001 ggcgattgac tattatgagc aggcactggt gattgcccgc gagatcggcg accggcgtag
    15061 cgagggtcaa acacttggta acttggggag tgcctgggat ggcctcggca agctgtaccg
    15121 ggcgattgac tattatgagc aggcactggt gattgcccgc gagatcggcg accggtgcgg
    15181 cgaagggaac gccctcggca acctggggaa tgcctgggcc gacctcaacg attcgcgccg
    15241 ggcgattgat tatttcgagc aggatttggt gattacccgt gagatcggcg accggcgcgg
    15301 tgaggggcaa gaccttggca accttgggaa tgcctggttt gccctcaaca atccgagccg
    15361 ggcgattgag tatcacgagc aagcgctggt gattgaccgc gagatcggcg accggcgcgg
    15421 cgaggggcaa gacctcggca acctggggaa tgcctgggct gccctcaacg atccgtgccg
    15481 ggcgattgac tattatgggc aggcgctggc gctggcccag gcaattggcg atcggcaggg
    15541 cgaagccaac cgctgctgga acctggggct gctgctggag cagcaggggg agctgacccg
    15601 cgccattgaa ttgatgcagg tgcaggtcga ttttttgcgc gccatcggcc acccagacgc
    15661 agaggaagca gatgcctatg tgcaagaaat ccgtcagagg ctgtagaaca ccctggttga
    15721 ttgcgctaaa gcaaaggaat tatgcgccag cagttactca attgactctg agttgtcacc
    15781 atatatgtac gtttaacctt tttatactat ggcagaccct tgacaggttt cgtttaccgc
    15841 cttatactat tactcgtccg accggtcggt cgacagatag gttgataaaa ggtaactact
    15901 cagctatgag cggatttggc cttcttctgg agtgcaggct ttagcctgca tctcatgcgg
    15961 gctgaagccc gcactccaca gaggagtgaa atactttcag ccaaatgagc ctctcatata
    16021 gcctgagcag taacgataaa agaatactta tggcaataga aacttacaac aaaatcctct
    16081 ctgctgctca tcgcctgttt gtaaaacagg gctataccgc cacctccatg cgccaggtgg
    16141 ccgaggcggc tgggattggc aaagccacca tttatcacca ctttcccgac aaacagtcga
    16201 ttgtgatggc gctgctgcaa cacaacctca cgcgcatgga agaggccttg cggcttgtcc
    16261 gcgccgaaaa ccacccgcgc cgccgcatcc aggtggcagc tgaggccagt gttgattttc
    16321 ttttcgagtc agtcgacatc ctgcaaatcg tccggcgcga aattcccggc ggccgcgacc
    16381 agatgcaggg cggttttgcc agattctttc aggaatacat ggccttgctg gccgatgcca
    16441 tccagcgtgg catcgaacag ggcatcttcc gcccggttga cccggtcaat agcgcgcgcg
    16501 tgctgatgac catgatccag ggcacttttg cgatggctta tctgatcaac gaacgcgccc
    16561 aaacgcccca acaggctgcc actgacctgt tggatattta ttttcagggc ctggatgccc
    16621 gttaagcgct ctttttttat ctattttatc gaccgaacgg tcggtctatt cgttgaaggc
    16681 aggagtctca tgtcagaaaa agccatctct gcccgcacaa aatttggcct ggtactgcac
    16741 tctccaacct ttgtggtgct ctggttgagt gaagccttat cactgattgg cgaccggctg
    16801 atcatggttg ccctggtgac cctggtctat gatcgcaccg gctcggccag cgcggtgggg
    16861 ttgttgatgc tgtgtaaagc catcccggcc ctggcgctgg gcagcctggc tggggcctgc
    16921 gttgaccgct ggaattgcaa gtggatcatg gtcatcgcta acctgcttca gggattgctt
    16981 gttttttgtc taccactctc gacgggcatt gtgatcatct ttgttatcta cctggcaatg
    17041 tcgacggtga accagttctt tgtcccggcg cgttctgcca ccatcccgga cctggtggcg
    17101 caagaagctc tgctatcagc taactcgctg tttgccattt cattcgtcat cgcgatggcg
    17161 attggacccg ccatcggcac ctggatcact gagcgtgtca gcctgaacgc tgccttctac
    17221 acagacgccg ccaccttcct cgtcccggcg ctggctgtca ctttcctggt cattccacgc
    17281 caatgccgga ctgccgcaaa acctggcctg agcagtgatc tacgggctgg gttggttttt
    17341 gcccgctctc aaccagctgt gctggcagcc ctggctacct gcagcgccgc cttcctggtc
    17401 atcggtacca tttcggtcac cggggtggtc ataacccgtg aaatcctgaa tgtcgattcc
    17461 agcaagtttg gcgttatgat gtcagcgctg gggattggta tgttgtgcgg tgcagtcgcc
    17521 acgggctggt tggggcggcg ctttacgtat gttcagttga gcgtggcggg ggttgttctg
    17581 atggcgctgg gaatgacggc cttaccctgg tcttcgagcc tggttatggc ttcctcgttt
    17641 acagccgcca ttggcctggg tatgatcatc gtgcaggtaa acggccagac cctgctgcaa
    17701 accatcgcac ctgaaatgcg cggacgactg atgggtatct cacaaaccct gactggcagt
    17761 gtcacattct tggcttcggc cctggttggg ctgctggttg agcatctaaa cgtaatcatc
    17821 gtgatgggga gtgtcggcat gatcacattg gctgtggctg ccacggtcgc catcttatat
    17881 cacaacactt caatgtgaat ggagtaccga aaatgcagta catcaaatct ctcacagaat
    17941 tgaaccaggc tgatctatcc atcgccgggg gaaagggcgc taatctgggt gcactgatcc
    18001 aggccgggct gcccgtccca cccggatttt gcgtcataac ccctgcctac cgtgcttttg
    18061 tggaggcaaa caagctggag ctggaaatcc gtcaaattgt cgatgcgacc cgtatggatg
    18121 atccggccgc gttggatgca tcctccgacg cgatccgtgc ctgtttccag cagggagaga
    18181 tgcccctggc tctggcggac gaaatctgcc gggcgtatgc caatttgcaa aacccacccg
    18241 gcttggtcgc cgtccgctcc tcagccactg ccgaggattt gcccgattta tcttttgccg
    18301 gccagcagga tacttacctg aacatcctcg gcgagccggc tcttctggat gccgtcatcc
    18361 gctgttgggc cagcctgtgg acggcacggg caatcggcta ccgggcgcgc aaccagatct
    18421 ctcaggacga ggtcgctctg gcggtagtca tccagcagat ggtgcagagc gaggctgcgg
    18481 gggttttgtt caccgccaac ccgctgaccg gaaaacgcag cgaaacggtc attgatgcca
    18541 ccctggggtt gggcgaagcg ctcgtctctg gtcaggtgga acccgaccat tacgtagtaa
    18601 ctggctccgg ggagaaaatt ctgcacaaga ctctgggtgc caaggcactc tccattcagg
    18661 gtcaggtcgg gggtggcacc gtcaccctcc accataccgc tgccagccac caggccctgc
    18721 ccgacgatca gattctcgcc ctggcacagc ttggtcaaca ggcacaaaat ttttttggcg
    18781 gtccgcagga tatggaatgg gcctggtcgg agggacgctt gtacgtggtg cagtcgcgct
    18841 cggtgacctc gctatatccc ctaccagccg gtctgaagga tgaaccagtt gaggtgctgc
    18901 tgtcctttgg cgtttggcag ggaatgctcg acccatatac ccccctcggg caggacgtct
    18961 tcagctcact gatccccggg atattgcagt tgttcgggcg aaaaatcaat gcgagggaac
    19021 agcgtgtttt tctcagtgcc ggggaacgcc tttacgtgaa catgaccggg ctgctctcca
    19081 accccattgg gcgcaaagtg gtcaatctgt ttatcggggc aattgaccct gtcagcagcg
    19141 ccatcctgac tgaactgctc aaggatgcgc gctttggcgt caaaaccagt ctcagcctga
    19201 aggagcgttt ccaactttta cgtgtagtca ggcctctggc cggaaatgtg ctgtttaacc
    19261 tcctttggcc cgcccgcggg cgcgcccggc tggaacggca gattgactcg gtactgaccg
    19321 aagtgcaagg ctcctgtgac gcagctcaaa acctggccga attgatcgaa tccatcgatg
    19381 ccgtcgcctt caaaatgccg caacagatgt tcccccgctt ggtggggggc atcgccgcgg
    19441 gacaagcccc ttaccaaaac ttgctcctac gggccagtca aatccccggt gcaccggaac
    19501 tgctcatgga aatggcgcgc ggcctgccac acaacgtgac gacgcagatg gatctggcgc
    19561 tttggcgtac cgcccaggtc atccgtgccg atgatcacgc ggcagctcac ttttcctcaa
    19621 cagaagttgg caggttggtg atggaatatc attccgaaaa gctacctgct gccgcccaga
    19681 ttgccatccg ctccttttta tccacctatg gcatgcgcgg catcggtgaa attgacctgg
    19741 ggcgcccgcg ctggcgtgaa aacccaaccg gccttttcca ggtgttgaaa agttacctgc
    19801 aaattgaccc ggcactctcg cccgaggcgg tttttcagcg cggtgccggc aagatctggc
    19861 aggtgcaggc gcagcttgag cagatttttt cggaggcgcc aaaaggaaaa aggatcagcc
    19921 cgcacttggt caggttgctg cttgaacgct tccgcgagtt gggaggtctg cgtgagacgc
    19981 ccaaattttt cgtcgtgcgc ctgttgggaa tttaccgcga agcactactg gccgccggtc
    20041 aaaaattggt tgaggccggc aggctgactg cggctgagga tgtatttttc ctgcatctgt
    20101 gggagttgaa aactctgggc gatagcgaaa cccggcactg gcaaaccttg attgcagagc
    20161 gccgggcaac caacgcgcgc gaggcgcgcc gcaggcgaat tccgcgcatc atgttgagcg
    20221 atgggacggc tttttacgat gcgccttcaa ccggggaggc tgccaatacg ctctccggcg
    20281 ccccggtttc ggccggcgtg gttgagggtg tggtacgcgt tgtgtttaac ccgcatgggg
    20341 ttcaactcac ccccggcgag atcctggtct gcccggccac tgacccagcc tggacgccgc
    20401 tctttttggc tgctggcggt ctggtgatgg aggtgggcgg gatgatgacc catggctcgg
    20461 tggtcgcccg cgaatatggc attcctgcgg tagtcggcgt cagccaggct acaacccgcc
    20521 tacaaaccgg tcaacgcgtg cgcgtggatg gtggtagcgg agtgattaca gtgttggagg
    20581 accagacata aacttaggag tacgaggcca ttattcaagt ccacattaga cccggtatcc
    20641 aataattcta aaaaatccag caccttaaaa aatgtagatc agggtatcct taagcttacc
    20701 atcccatttt cggtaaaaac cgccccccac tcaacagact ttaggaaaaa ccaaacgtgg
    20761 gcaaatttct acaaacagaa aaaccaaaac aagtcgcgtt taagcaaatt tccgccacaa
    20821 tctccaatgc ggcaaaagct gaaggaatct ataagaatca tgcttacccg ttttgcttgc
    20881 cgcgcgaact ggcagaagag aatctttatc ccccaatccg caccaccatt cgagaatatt
    20941 tcgagcaaaa taaaatcaag tggcacgatg gacaaaacgg gaatcccagt aaccatatgt
    21001 gcgattccca ggtctgctgc gccaacttcc tctttccgtt cgcagataaa cctcaagccc
    21061 tcgctgctct gttgaagccc atttttccag atttacatga aatgctgccc atcgaggatg
    21121 gcttgtatgt cgcattcgag tggataggac aggaaaatta cctgcatgaa aaagtaccca
    21181 gaaatggtca acggacgcgc ggagcgaact gcaccagcgc agacgccgct gttcgtttca
    21241 agcgcgcgga tgggcgcgaa caaatcagct tgattgaatg gaagtacacc gagtcataca
    21301 gccgcatgag cctggaggtt gcagccagtg gccaaagccg agttgagatt taccgctggt
    21361 tatttgagca gcctgactgc cctatcgaca aaacccggct gcctggtttt gaggcgctgt
    21421 tctttgaacc tttctaccag ttcatgcgcc agcaattact ggcccacgag atggaaaaag
    21481 cacatgagct gggagctgat atcgtcagcc tgcttcatat ctcgccggcc cataatttgg
    21541 atttcagaac gatcacctca ccgcgattgg aatcccgcgg cttatcagca acagatgtct
    21601 ggaaaacgct ggtgaccctc ccaggccgtt ttataagcat cagtacggaa tcgctgtttg
    21661 ggcagttgga tctgagttgt tatcctgaac tgctcgagtg gcagacttac attcaagcac
    21721 gatacacctg gatgtagcgg gggttggatt cgaattcatc cctcgacggg accaacgacc
    21781 tccaaacaaa aagacctggc atataccagg tctttgcagg tagcaggggg ggggcgaacc
    21841 aacgaccctc gggtcaggaa ggcgatgctc cgcgcccgct gcaccaggcg gaccggtgcg
    21901 gtactggaat tagccaatgg gggatgggag cgccggtccc ttattcaagc acggcgcgga
    21961 tgcggcgtat gccctgtccg acagcctctt ccttggtaat gcggaaatgt cccattgcac
    22021 cggtgcgggt tacatgcggc ccgccacaga cttccttcga aaaatccccg attgaatata
    22081 ctttgacttg ctcgccatat ttctcgctaa agaatgccag cgcgccgctt tccatcgcct
    22141 gctgcagggt ttggatttcc atgttcacag gcaggtcagc cgcaatttgg tcattgacaa
    22201 tgcactctac ctcggcgagc tgttctggtg tgacccgctc ggggtgagaa aagtcgaagc
    22261 gcaggcgctc cggcgtgatg ttggaaccct tctgctgcac gtgggtgccc aacacggtgc
    22321 gcaatgcttt gtgcagcagg tgggtggcag tgtgcaggct ggtggtcttt tccgcgtggt
    22381 cggccaggcc gcctttgaat ttctggtcaa gattctggcg ggaggattcg cgatgctcgg
    22441 cgtagcagcg ctggaaacct tcctcgtcta ccggcaggcc attctcgcgt gccagttcct
    22501 gggtcaattc caacgggaaa ccgtaggtct cgaacaggtc aaaggcatct tcgccgctca
    22561 ccaggtttat gtgattctcc ttgagatggt cgaacacctt gttcacctgt cgcagcccgc
    22621 gctcgagggt atgtttaaat ttggtttcct ctgcatccag cgcgccggca atgacctcgc
    22681 gctggcgttc aagctcagga taagtggcct gcatcatctc aaccacggtg catgatagag
    22741 cgcccaggaa gttatcatgg atacccagct cgcgtccaaa gcgcaccgag cggcgaatca
    22801 tgcggcgcac cacatacccc gactccacat tggaaggccg ggcgccgtcg gctatggcga
    22861 aggacgcagc ccgcaggtga tcggcgatca cacggaaggg cgacgggttt tctgcatagc
    22921 tgcgcccaga gagctcctcg attttttgga ccaatgacca cagggtctcg atcttgaaga
    22981 tatcatccaa tccttgaaga acagcatggg tgcgttctaa tccgagaccg gtgtccacgt
    23041 tttgttgttt caggcgggtg aaattgccgt cggctttctt gtcatattcc atgaatacgt
    23101 tgttccatat ctcgaaccat tttccgcaag agcagccggg tccgcagcca ggatgatcgg
    23161 ggcggccggt gtcatagaat atttctgaat ccggtccaca agggccggtc tggccggcgg
    23221 gtccccacca gttatccttt tttggcaagt aatggatgcg atcaaccggt atccccaatc
    23281 gcttccagat gcctgccgct tcgtcatcac gtggagcgtc ggcgtctccg gcaaatacgg
    23341 tcactgacag gctcgaaggt tgtatgccca gggtgcgggt gagaaattcg taactccagg
    23401 caagcgattc ctccttgaag taatcgccga gagaccagtt tcccatcatt tcaaaaaagg
    23461 tcagatgtga agcatctcct acctcatcga tatcattcgt gcggatgcat ttttgaaagt
    23521 ccaccaggcg cttgcccagg ggatgcggct cacccagcag aaacggcacc aggggatgca
    23581 tgccggcggt ggtaaagagg acggtggggt cgttttccgg cagcaaaggc gcagagggga
    23641 tgcgccggtg accgcgtgct tcgaaaaatt tcaggtattc ttcgcgtaat tgattgatgt
    23701 tcatggttag tctcctaaat aaaaaaatcc tcagcttttg ctgaggacaa gagttgcttg
    23761 tggtaccacc tcgctttaca tggacctcac gacccatgtc ttgtgcgccg ctgagaacgg
    23821 tgctgctctg taacgggaac tcccggcgag gtctactcac cacgaaggtt ttcgagtcgc
    23881 atctcgaggg tggcgaacag gctgtttatt ggcaccggtc tctcaccatt tgaccggctc
    23941 tctgggcgca cgtacagcat gtttggtccc tgtccacaac gttgaggaaa ttatacccta
    24001 cgtcgcaaaa gttttcaaga atttaagttc attgggattt cccgtaaaag aagttatatg
    24061 tgcgaatagt tgtaccttga ttgtctaact acacctcaga caccccttgt gtgctaatcc
    24121 cccatacttt ggggattggg agatcaagaa acctgggatc tcagggactc aggaatttaa
    24181 gggtatggga acaaataaac tcagggaatt ggagcgtcag gaagttttgg ggattaaggc
    24241 tcaatagaac ttacagatcc aggaaatcag gggggctgga aatggaaaat tgtaatctga
    24301 atagatttgg aaatctgagg gtttatctgt atctttagga accagtaccc gggtatgagg
    24361 gagcaggtaa aaatagagca atgtaaaatt gctttttgtg ttttaacgta atggtaaagg
    24421 tggcgtaacg cctgtttata ttccctggag accttaaagc cagggagatc aggaaaaatt
    24481 gacctccgtt ttaaatttaa tgtcaatctc ctatcgccgg gccaatcaaa aaaccatagc
    24541 ccggaggact atggttcttc tgaggtagta ggggttagat tcgaattcat ccctcgacgg
    24601 ggccaacgac ctccaaacaa aaagacctgg catgttgtac caggtctttg taggtagcgg
    24661 gggtaagatt cgaattcatc cctcgacggg gccaacgacc tccaaacaaa aagacctggc
    24721 atgttgtacc aggtctttgt aggtagcggg ggttggattc gaaccaacga cctccgggtt
    24781 atgagagatt ttcctggttt cggaacgttt ttcactcagg ctccgcattt tagcctggcc
    24841 tgagggacgc tcttctgaag aggccggagg atctttggtt acgagttatt agtcgtatga
    24901 tagcgacttt ttatgacagg gtcaagtagt agttttttcg cgacccggag gacgatggtt
    24961 gcaaattagt gaacgaactt ccaaaaatca cgaccttttc gatcaatcgt tcatatgcaa
    25021 attcttaaga taattcaagt tcttcattgt cacaaaaggt caatcgttgc caaaaaagga
    25081 tgtccgtaaa catgtttaaa tcatccatta ttgaaattag tcgaaaatcg aatcatggcc
    25141 ttctatgtag aagagtacat acttgtactt gatctaattt catcttacct tacaggtctc
    25201 tttgacaccc tttaattctt attcacctgt aggtagtgcg tacagttcct caattgtcat
    25261 cccaaaggta tcacttacgt attgctccat cagatccacg gaatgctggt aaacatctac
    25321 gattgcggga tcgttgtaag ctttaatgaa gtattcttcc acggtcttat cagggtagct
    25381 gcaccagata tcttgatgct cgccagtatc gatgaaatat tcaaaactcc cacctccctg
    25441 gctgttatcg tccacgctgg cgataaattt cttttccgcc gcgtgccagg tgacaacaat
    25501 gcgatattca tcacttatgg gggtaaaaaa tcgaacatcg aactccttct ccccccattc
    25561 ggggcggtgg acttccatgt tgacgtcttt ttcgccccgg tattcatata aatagaccgc
    25621 gttgggctcg tccgcgaaga acccgaagct cttgagagat ggcggcgcgt acggtagtgc
    25681 gtacaatttg gcagcggtca tatcgaaggt gtctttgacg atggcgtgaa aaacgcgcat
    25741 gggcgcgagc aacggatcct cgtcttccat gacctcaaat gccgcgcgca ccacctgttc
    25801 gatactcgcg cggtcttctt cactgatgtt gatgtcacca ctgtgggcgt cgtaaacgta
    25861 gtttaccggg ggttcgcctt ccttgtgcat catgaagacg taggcatcga gatcgccata
    25921 gtaacccagt aacaccttat gaccgctccc tgtatacatt gaggtgcgga tggtattggt
    25981 gtgccattcc tcttgaggtt gcccccaagc ggcatggtga atggtgaatg aagcgctctc
    26041 gctgtcatag actgcttggt ggctgttgac gtccaggtta aatccgagtt cagccagggt
    26101 tgtggcaatg atgaaatcct tgtcttcgag gtttgggtaa attgtctcga gggggtacag
    26161 gtcttcaatg gacgagtttg ctagatggag ctggcgcagg ttggtcagcc cggctagggg
    26221 agtgaggtca gagatggtcg aattatcgag caagaggact tgtaaattga ccaggttcga
    26281 gagcacgctg taatcctgcg ctttgctccc tgaaagcatc agcaccttaa gcttggtcag
    26341 ccctgcaagc ggcgaaacat ccgcaacggg gttaccagcc agcgacaagg tggtcagttt
    26401 ggtcagttcc tgcaatgggg aaatgtcggt gacggcatga gaggagaggt cgagggtttc
    26461 aaggctggtg aagttctcca gtccgctgag atccttgatc ggtgtctctt ctgaaatgta
    26521 gctttgtatt tcattgctga aattcatcct cgtcaccgcc tgtgcctcag ccaaggtaat
    26581 gtcgccttct gttttgccca ttgcgccgcg tatcatcgct tccagcactg gatcagcaaa
    26641 tgttacgacg acgggcgccg atgtcccaac tggagacgag gtcgtcgtga cctccacagc
    26701 cgggtttgat gaggtcgttg tcacagcggg cgtgctacag gctgcaaggg ttatggtcag
    26761 caaaacggtt atcagaatat tatgaggttt catcattgaa agtaatcctt gatttatttt
    26821 gagtgaaaat tgtgaaggtg ggccaacaga taggcatcct ttttgttcat aaaattatgg
    26881 gggtttttgc ccttttgtat tttactctat ccacctgatc acacgttaag ctgtgcaatt
    26941 agaaggaaat cacatgttga catttggctt tgatgaaaaa aatgacccac ctcggtgcgc
    27001 atgcagagca gaggcgtggt gggtactgtt ggattgatta taaccaggtt tacccataaa
    27061 gacccttttt ggcaaactaa ctcgcaatca aaatccgaat aaggtcattt tgtatcggga
    27121 attagcttaa attcaatctt tgcacctaac gcttcaacga tcttgcttag gaaggaaaaa
    27181 ctaggagcgc tggtgccatt ttccagtctg gcgatggaag gctgccgggt gccaaccatc
    27241 tcagcaagtt gagcttgagt taaaccccta aacatgcgga gccgagcaac ttggtagccg
    27301 ggttccaatt cctcggccaa agacaggaaa tccgaggctt taatcttttc agactcccaa
    27361 tcttcaaatt taagcaattt actcccttcc attccatatc ctgtgaattt ttcaggtcta
    27421 gatgatcaat gctcgcaaga actaatccac ctatactaat cagatctgtc gataaagaaa
    27481 tctaaattat cataatccaa atttctttat tcgcgtcatt tatataaaag ctgacaaaat
    27541 tcatatttgt tacctttcat gaaaataacc ttttgtttca ttcgccagtt aacaccgtct
    27601 gcgtccaggc tgacgtgacg cagacgtctt gctgacacgc cgcagacgtt gacgcagacg
    27661 ctgctgcgga cgttgatcga acaggaaggt tcccaaggtg ttctttctga tggagggggt
    27721 aggaaatatc aactgaagaa gaatcgcggg aatggtcact ttttgccgag ggcaactgca
    27781 aaaattctgc gttattctgg atcaaatctt tcgatcaagc ggttgtaaat tggctatttt
    27841 tgtgcacaaa caaatcgccc gctttttttg tgcacaaaat atctccaata cagcggggaa
    27901 cggtgtggca cttcgttctt gccacttcga tcccctagag tcttaagggg gaacgggtca
    27961 acggagtgac acatcgttct ctgttggatg gtctttgctg gttttaagaa tatcactcaa
    28021 ccgccaggga cgatattccc tgacctcaac gctcatattg atatggtcgg gtcccaagtt
    28081 agaaccctca ggaggcggtg gaacattatg aatatgacca tgaaaattga tccatccatc
    28141 ttccaactga acaatcggac ggtgtgaaaa aatcagcttg atttgactta ctacttcaac
    28201 ttgggatgaa tttataatca gggtcacgcc atgggcttca taaaatgacc ggctcaaccg
    28261 atcgtggttt cccttgatta agaacaatct accattcaaa ctactggtaa aagctcgaga
    28321 ttgaccccga ctacatatag aatttaaatt gtggcattgg ttcacggtca gaaggaattt
    28381 ccctcataat ctctccgacg atatctgaga atcgcagagt aatcggtgct tttccaccca
    28441 gccgtgatga attccagttc attttggtca ttatgagcac ttcctttaga agtgtttctc
    28501 tcggcgtgtc ttgcccaaca tggtcagtga ttagaatagg cgaaggtaca tgtagaacat
    28561 gagattgtcc taactctgat ataaaccctg ttgtataaag gaaatccatg tcgcctacca
    28621 tgaatcgagt gccacgtaag ggtgggtagc tacttgttgt aagtaagcga atatgactct
    28681 gtcgggtatc aaatgacatc aaatcaaatt tttttactcg ttggcctaga gcttgcttga
    28741 atccatcttt ttctcccggc caaaacgtag aagatttatg aataactact cgttgaggag
    28801 tctgtcccat ttctttctca taccggtcca aaacaatatt gattaaatta cttgcgtccc
    28861 tgtcgtttaa atgcggagat ctggaattca ttcgatatga gttccactgg aattcaggtc
    28921 ctctaagaat aagaccatca ccatgttcgt caaatgcctg aacaagactc gtttgcatgg
    28981 aattaaattt tgatccaagg ggacgataga acgcaattcc cacataacag gtcccaggtg
    29041 tcaatccaat tggcccccaa ggaattccac cagctttaaa atagagacca gtgaaaaaat
    29101 tccacgctac tttggatgga tcatcttttt ccctaccgtc aatagtctgt tgccgaagga
    29161 attgtgtggg tatctgatac ttcatcgcta gtgctttgaa tgccctgcga agatccctgt
    29221 gcactaatcc tttttcttga tctcggtaat caacaccctt gcattttccg tatatgctgt
    29281 caggcaatgc cacaataatg tattgtggtg gctggtcttt ttttgacaat aaatcgagtt
    29341 tttcttctaa tatttctaac agaaactcaa atctctcttt aggtttcttc tccaagattt
    29401 tatctatttc cttttgcgta agttgaccat tccaatcttg atcaaaaatt aatcttgaga
    29461 aaaacccgcg gtcttccatg taacctggaa attctacgtg atcttcgtca cataaaacac
    29521 cttcagaagt ttttttcagc cagctcttcg cagaatcgat tgtgtcagcg cttccaataa
    29581 accctatgcg taccgtatcg gggtgcttgc gttttgggct aaaagatttt ggaccatatc
    29641 tagatatccc aattttggga tcaatatgtt ctctaccatc tgcaaattgt agtaaaggtt
    29701 cttcaaagta ttgacacttg attatcggtt ccattgattg gttattcatc aatatcatct
    29761 tctaccccct cgtcaagatc gtcgaacaat tcctcatttt caagaacatt ctgcagtttt
    29821 gcccacgtaa ataaatcctc tgcatattca acattagtaa atgttctcgc gtatttttct
    29881 ggaatacccg gccaattgat atcaccagaa agaagagtcg tagatataac aagggaagaa
    29941 gagccaagat ctataacgat tcttggttcg ttgttaccaa gaaaactcct ccagaaattt
    30001 atttcaccca atagatcgta attgaacatc ttacttttct tccgcgtaac tttagcgcca
    30061 attttttcag aatctaaagg caagttgcca tcttttgtca ctcgaaattc cggcctgagt
    30121 gcaaggcacc accgaccaga tgacacataa taggagcgaa ggctaattga gcggtgcagc
    30181 caataggatt ttggcagtcc agtctttttt gtgattggct gccaaaccac atgtttcttt
    30241 gcaacatttt gatttagagg cttatattct atatctttag gctggccagg ttgatcaggc
    30301 acaaagtaat atctgtaatg atcaggatcg tacactaagc ccttttgaga agtaaaaatt
    30361 tggagggtga tatttagtag ttcaacaaac caacgagact tgacgggatc atcccaccac
    30421 ttacgtgatg ctatctgcct aacggaattt aattctacga ccgtatggaa ggggctatcg
    30481 gcattcctgg gatcctgaaa tgtgtatagt ctatcctcct tgaggatata cggaagatga
    30541 atgtctggtt gagtaaacaa tggcaactgc tcaatcgcgt agttcgcgat agcatctttt
    30601 gtgggtgtag attggtagat atatacaggg gtctttatta cttcaagaag agacgaataa
    30661 acgctttcta taggatactt atcaacaaac tgatctcttt ttgttgtctt atcaatggca
    30721 ttacctaatg cagtctgaac aagtttttcg tgatcctctt tcattcccca taatcgctcg
    30781 acggtataca attgtggttt attatcaata attttatgat gttcaggaca aagcagcatc
    30841 aagttgcctt cttcatctct gcggttcaaa ggtaatgaga actttcccct ggggccatcc
    30901 tctcgttgtg caacaatatg agctacattg ctcagtataa cggtgctgtc atcactttct
    30961 tgcactgtca gcatagatgg acaacctgga aaagcgcatc tatttccgga tttttgtaag
    31021 agagctttta aatctgttgg agagattgtc ataatttact cctcaaaatt gttatgctcc
    31081 agtaaatcaa ccccatcgtt ctgaaaagac aaacctagta agaagattaa acaggatgtc
    31141 aaaatagatt tgttgtatca gtgattactg cgtatgcata tcttgtttca tcaataatat
    31201 tccttgatat acccgtacgc tttatcaaac aactcctgct ctttgtgcaa ctgatatttc
    31261 aatagcaccc tgcgcaacgc ctgtttgacc tcacgctcgc cggcgatggt ttgctgccaa
    31321 ccagggaagc ggacattgcg gacgatctcg tcaatgtcgg cgacaatgcg ttcgacaatt
    31381 accggagtct gagccgtctt ggtttcgttg aatagctcgg ttaatgcggc tttggcggat
    31441 tggcgttcct cttccgggtc cacatccttt tccgcttgca aaactttgcg ggcaatttca
    31501 agcagttgtt tcaggtaatc caggctgttg accagccctt gttccatgcg gttcttcaga
    31561 tcttcaagct gctttccgag cgcgataaat tttggattat tgccgtgctt tcggatgcgt
    31621 gcgatgacct tgatttccac ctcgcggatc ttctctggat cgcgttctcc gagcagttca
    31681 tccaggaaat ccgcgtccat gaccaatgta tccaggtcat cccgaacgcg ttcgacatga
    31741 acgttctcat gaatcaggtc gagggtcttg gctcccagtg catgccagag cagtttaccg
    31801 ctgccgctgg gcggctttac ggattcataa acctgtccca accagcgata atcatcttcg
    31861 taatctgaaa gcaccggatc cggcgatatc gcctcccaga gttgtgaaag cgttgagaag
    31921 tcgccggcga atttgtcccg cgtttcgtta tccggcaggc atgcctgggc tgccaccaac
    31981 ccttcccatc ctccgagggc gcgatccaca ttaggaaaat atgccaggca ggattgcatg
    32041 gcttcaggaa gctgcgcatg cagcgccttc aggttggtaa tcacctgctg catgttggct
    32101 tcgtcaaagg ccaacgttcg agccacatcg tcgaagacgc caatgtaatc cacaatcaag
    32161 ccataatcct tgccggggta gggacgattt acgcggcaga taccctgcaa caggttatgc
    32221 tccttcatcg gtttgtccag atacatcacc tgcagaatag gggcatcaaa cccggtcaaa
    32281 aggcgtgaag tcacaatgag gaaccttaac ggatctgccg gatcgcggaa acggtcgagc
    32341 aatctttcct catcttcttt actgcggtcg taggctttgt attccgtctc attgtgaaaa
    32401 acgctcatca cgatttcact ggcttccggt cgcaggattt catccatggc ttttttatac
    32461 agaacacagg cctggcgatc aaaggtaacc accatcgcct taaagccgtt gggttccacc
    32521 ttttcctgat aatgatccac gatatccgat acgatgcgct gcactcgttc tggcgctttg
    32581 actaataccg ccatgcgggc agcctggcgc gccagattcg cctgatcagc ttcggataaa
    32641 tgaccggtca gattggcata ggcttcatca atggattgtt tgtccacgcg cagttccacc
    32701 aagcgggatt caaagcgcaa gggcagagtg gcctggtcgc ggatggattc ctcaaaagaa
    32761 taacggctca ggtagccttt ttcatcctct tcagcgccga aggcaaaaaa agtattgcgg
    32821 tcgcgttgat ttatgggtgt gccggtcatg ccaaacagaa acgcattggg tagcgcttcg
    32881 cgcattttcc tgcccaggtc gccttcctgg gtgcggtgag cttcatccac cagggcaatg
    32941 atattgtcgc ggttgttcag aacgccgtcc gcttcgccaa acttgtggat ggtggtaatg
    33001 atgaccttgc gcacgtcctg agccaacagg gtacgcagtt cttcacggct gtcggttgag
    33061 atcaggttgg gaacatctgc tgcgttgaat gtagctgtaa tctgcgtatc cagatcgata
    33121 cgatccacca cgatcagcac ggtaggattt ttcagcgccg tatgcatgcg cagtttctgg
    33181 gcggcaaata ccatcagcaa tgatttgcct gacccctgaa aatgccagat caagcctttc
    33241 ttcacccgac ccagcactac ccgttcggcg atctgattgg cagcgtgata ttgttggaag
    33301 cggcaaatga ccttggtctt gcgatgtttc ttgtcggtgg caaacagggt gaagtttttg
    33361 aggatatcca gaatgatcgc cgggcgcagc atgcccagcg cagcggtttg cagttcattc
    33421 aaaccggaat attcattgtt atcttcttca cgccatggtg cccagctttc cagcggctgc
    33481 cggatagcgc catagcgata ggttttccct tcggtggcaa atgaaaatac attcggcgca
    33541 aagaacgctg gcacgttgac ctcataatca tcctgcacct gtaccgcgcc atctacccat
    33601 gagatggccg ggcgggtggg agttttggct tcgccgatca ccagcggaat gccattcaca
    33661 aacatcacca ggtcaaagcg acgggtttgc gagccggcct ggaagatgac ctggttggtg
    33721 aggacgcatt gattattgcc gatattctca aaatcgatca accgaaccga cgtatgctca
    33781 tgattgggtc caaacggcat cgaacgctcg ccgcgcagcc aagtggtgaa ctcctcattg
    33841 gcgcgcacca ggccgtcgct tttaaccgac agcagcacgg cacgcaggcg gtagagcacc
    33901 tcgtcggcgc gctcgggctg ctcggcgatc tccggattca ggcggatcaa cgccgcgcgc
    33961 agctcatctt ccagaagcac atcctgggtg gatcgatgca gtttatccgc gggtttgtaa
    34021 acccaaccct gccctctggc aagctgcgcc agtgcgccgg cgtataaccc gggctgctcg
    34081 ccaatttgct tgccggtttt gccgcacagc agatcgcgga tgaagagttc gactgagttg
    34141 gattcgttaa acatcattga ccacctaaga gtttctgcaa caattctgct ttaacaattt
    34201 ttgctttttc aattgatcgt ttctgttcta gatagcttga attaacttct atcaattttc
    34261 tccccatttc ttcccgaaga gtaacctcgg gtaagcaatc taaaacatat tcatcgagga
    34321 agtcctttgg cacccttaat tgacctgctg tcccacgcat aaatcgtctt gcttgttggc
    34381 gaaatttttt attcatggta aggaaatata taaaggctgt gtctctggaa tccatggggc
    34441 gtaagacatg aaattcagta cttccaaagc caataccatt aatcaaattt agagcaagtg
    34501 cccctttgcc attttccata catggagtga ttttcgcaaa taaaatatcg ccatcctgaa
    34561 agacggtgta acctttctta acctctccat acatttttga ctgctgattt atgatttccc
    34621 catcttcaga aacgtcagcc atggttatga aagacacagg ttcttcatct ttacattttg
    34681 gtttagaaga agggttcacc tccgcaattt ctgagagtaa tgttggttgt ccactatcgc
    34741 gacaacggaa gaaactctct tcttctactg agtcctttag gctaattatc ctttgcaatg
    34801 taacaaggtt tttcgtgatg acttcatctg cagcccagag gatctctgca atgcggcgct
    34861 gctcgtccag cggtggtagg gggaatttat aacgtgctaa atctgaccaa ttaatataag
    34921 gatttactga cccttttgaa ttctttatag aga
//
LOCUS       DAOPVS010000013        34055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1208_length_34055_cov_59.030118, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000013 DAOPVS010000000
VERSION     DAOPVS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 34055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34055
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_34055_cov_59.030118"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKL11_02570"
     CDS             <1..231
                     /locus_tag="PKL11_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNZ12512.1"
                     /translation="KDKRGQIKPILTRLQREFNLSTAEVGLQDEWQETILACAIVSTD
                     KVHNQQVLQQVVDYTARHWPEAEILKHTIETW"
     gene            248..1039
                     /locus_tag="PKL11_02575"
     CDS             248..1039
                     /locus_tag="PKL11_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12513.1"
                     /translation="MDLGLKNKIALVTGASRGLGFATARLLAQEGARVAINGRNAEAL
                     EKAAAQIAAETGAETLALAGDVVDPATPPRLMEEIKAAWGGLDLLVANAGGPPAGAFE
                     SFDDEVWQKAVELSFLSQVRLIRAALPLLKQSDCASVLTITSYSVKQPIPNLVLSNSI
                     RAATVGLTKSLALELGGQGIRFNSILPGWTETERVVNLMSARAQSSGRSIAEETARQA
                     ADSPLGRMGQPEEFGRAAVFLLSPAASYITGVMLTVDGGMYKGTL"
     gene            complement(1036..1890)
                     /locus_tag="PKL11_02580"
     CDS             complement(1036..1890)
                     /locus_tag="PKL11_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ12514.1"
                     /translation="MSKIAIITDTDSSLSADVAHSYNIQLVPIGINFGDESFTTGVDI
                     DDARLFERVNRTGKLPTTAAPSPEQFLRAYQQAFEGGADAIICYCISAKMSATYGAAV
                     LAREMLPDREIEVIDTNSVSLGQGFMALAAADVIHSGGSMTQALEVSQSIGQRALLFG
                     ALSTLKFLVLGGRLTKFQANMAGLLDIRPVLTLNDGRLEMLERTRTHKKALARMIELM
                     SNALNGRPLEKLGIVHTDCLDEAQDFAEQVRTALPCPAEIMISEFTPGLSVHTGPGML
                     GLLGVPAR"
     gene            1976..3877
                     /locus_tag="PKL11_02585"
     CDS             1976..3877
                     /locus_tag="PKL11_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNZ12515.1"
                     /translation="MTKRLSESAEIIQEKLERILPTVTKPGRYTGGEYNQVVKDWQAV
                     QTRVALAFPDIYDLGLPNLGLAIFYEALNDRPDVLAERVYLPWLDMEAALRSAGVPLY
                     SLETHHAIADFDILGITLPYETLYTNTLNLLDLAGIPLLSKERTAQHPLVIAGGHACY
                     NPEPMADYIDAFAIGDGEEVFHDIVNVQQAWAKSGADRAALLRALAQIPGVYVPALYA
                     PRYHADGTLAGVEPLDNSAPRQVLKRVVGQLPPAPTRFIVPSIDVVHNRVSVEIMRGC
                     TRGCRFCHAGMVNRPVRERSVAEVVQTIQTAVKQTGFEEIALLSLSSSDYSRIVELVE
                     QVREKVTGPHLTVSLPSLRIESFSVELMERLRGGRQGSFTLAPEAATDRMRNTINKPI
                     QDQQLLETAAELYGRGWPTLKLYFMIGHPSETLEDVQAIADLCLEVMAVGRKAIGRRA
                     SLHVGVSTFVPKPHTPFQWVACDAPEMIEAKIQLLRRALRSPGLKMNWTDPRETLLEA
                     WLSRGDRRLGEVIWRAWQHGAKFDAWQDQFNFEAWQTAFEESGLNPADYTHRDRAEDE
                     VFAWDHISPGVSKAYLLDEYHASLEGRLTGDCREQCYACGILPQFAAMRRQLPKSAWF
                     CPGGDPAKA"
     gene            3885..4517
                     /locus_tag="PKL11_02590"
     CDS             3885..4517
                     /locus_tag="PKL11_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNZ12516.1"
                     /translation="MKRIRIHYAKTEGLRYTSNLDMQKVWERALRRAGLPLYYSQGFH
                     PQPRIQQACPLPLGLLSRCEIVDVWLEDDTCTLEGTLKALQPALPTGIELLKLEEIDP
                     AAPALQKLAGSADYDITLLDPFPVDELERRTAALLGAEALIRERRGKNYDLRPLVEDL
                     AVVPGEPLRLSARLSVRDGATGRPDELLAALGLDPFGARIVRVRLILAEA"
     gene            4575..5372
                     /locus_tag="PKL11_02595"
     CDS             4575..5372
                     /locus_tag="PKL11_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNZ12517.1"
                     /translation="MKFAVNYSHAAADLLEAGQIDFDLFKFPDWPDFVPQNGLIRKPY
                     VHYTLAAGNGSQAAVDWQMIAGLRARTASPLVNTHLMAPAGLEPDDPAQVEAAIQGMV
                     ADLQDLVARFGAENVVVENMPFYVQNRHMRVAIEPATIQRVVGESGCKFLLDTAHARI
                     TSAALGIDVYDYINALPVNRLGELHVTGVGWHNGERIDHLELLAEDWEVFDWVMAHIA
                     AGHWPQPKVVSFEYGGAGEVFRWRTEVRVLAEQTPRLFATVQAARHN"
     gene            5380..6021
                     /locus_tag="PKL11_02600"
     CDS             5380..6021
                     /locus_tag="PKL11_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNZ12518.1"
                     /translation="MTLETIIRQCSDPTCRFRYPQAAGPARPGHPCPRCGAPTEIAPL
                     PLLEPDDNPRSLEASGPPVEALLDNVRSTFNVGAMFRTADGAGVSHLHLCGVTPTPEN
                     PRVGKTALGAEYAVPWTWHPNALDAALAGKARGLRLWALEVGPHALALPEVRSVISDE
                     PILLVAGGEVSGVDPAILALCERTLWIPMQGFKRTLNVAIAFGIAVYGLRFAG"
     gene            6331..7164
                     /locus_tag="PKL11_02605"
     CDS             6331..7164
                     /locus_tag="PKL11_02605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12519.1"
                     /translation="MKKSVWAGKLTLITGGSSGIGLALAKKLAAAGAQVHLLARRLDQ
                     LESAKAEVEVVRADPGQTVGILAGNVAEAEAITERLHEFEQTVGTPDLLINSAGITHP
                     AEFQDTDLDTFRRLMEVNYLGTVHVTRAFIKGMLARGAGQIINISSAAGFVGTYGYTA
                     YSGSKFAVRGFTDALRAEVKPRGLKVSIVFPPDTDTPQLAWETPLKPAVTREFSKAAQ
                     VLSADKVAEITLRAAGRGQYIIVPGFDTHLFYHLNNFLGPLAFNVIDLMVADAIKKAK
                     K"
     gene            7352..8278
                     /gene="argF"
                     /locus_tag="PKL11_02610"
     CDS             7352..8278
                     /gene="argF"
                     /locus_tag="PKL11_02610"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNZ12520.1"
                     /translation="MRKDFLAISDYTPAELQAMLDLAVRLKKELRSGGNAPLLQGKVL
                     GMIFQKPSLRTRVSFDMAMRQLGGDALYLSPNEIGLGQRESIADVARVLSGYVDALMA
                     RVFAHDHVLELAKWASIPVINGLSDYNHPCQGMADALTIIEKFGSLKGLNVTYVGDGN
                     NVAISLMHICAKLGANFIIANPEDYDILPAAVEIARGFAATSGSKLTFLRDPHEAVRG
                     AHVIYTDTWTSMGQEAEGKKRELVFPPYQVNEKLVSEADKDVIVLHCLPAHRNHEITD
                     EVADGPHSMLFPQAHNRLHAQKAVLVTLFGKA"
     gene            8373..9683
                     /locus_tag="PKL11_02615"
     CDS             8373..9683
                     /locus_tag="PKL11_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ12521.1"
                     /translation="MDWTQHYQSHKMSAADAVRLIKSGQRVFMTGNCSVPHALLGALV
                     EYAPELHDVEICQALTVANGDYVTPAMEGHLRVNSLFISHNVRKAIWEGRADFTPVLL
                     SELPLLFKRGILPVDVALIHVSTPDEDGYCTLGVEAGLSITAAEAAKLRIAQVNRQMP
                     RLIGDTRMHISKIDAIVEVDEPLSELAMGEEGDQALVQKIAGYIAERIPDGATMQLGI
                     GAIPDAVLKYLFHKKDLGVHSELFSDGVIDLVEAGVLTGARKTLHTGKITAGFLLGTR
                     RLYQWADNNPLVEIQRTEHVNNPFIISQNRNMVAINSAIEVDFTGQVCADSIGTRFYS
                     GVGGQNDFIYGASLSEGGLPIIALPATTTLRDGTVVSRITPMLKPGAGITTGRNHVHY
                     VATEYGMVDLYGKSIRQRTQALISIAHPDFRDELTRQAKDLNYV"
     gene            complement(9743..11764)
                     /locus_tag="PKL11_02620"
     CDS             complement(9743..11764)
                     /locus_tag="PKL11_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ12522.1"
                     /translation="MLPKMLKLKPLSLYFTYLFMCSIGTIVLTLSAPSDLENQWLWGY
                     SLNRWLMIFAMIAVGLLAGAALYGISRGGRFSQQLEKLFQFSPVVSTLFFVLLLALAF
                     LLLMTFTTLLPNRFFFARLRPLLILALLVALGIAGYDLCVLRHRPWAVLTGLARSGIK
                     RIQQAAADTPRLFYLAMSGYRFLILPLALALPLLFGVALKYENPAGYAGLYTLMSDEL
                     VSAGFRLPQTVPYYGPGGMPFAYPPVGFYLMGLVTSLFNISDFSYLRFAPPLFSWLAL
                     IPLALLTFELTRSRGAAVIAALVVAASQRIFYIHGTSGGMVRALAFLFALWGVYFYLR
                     SAQFSRRRPALFAGIMFALTVLTHLGYAEFAFLFVVAYTLTHLNRKPTYSTALITSLV
                     VLALVAPWVTVMVQQHGWQIFSGAFQSHGNNYFLSIFQDPARLLPWFESSLHAIYKLQ
                     FVWGLIVLALIFAAINRASLLPVWFGLILLLMSEGDRYLITSGAVLIGMLSSALAERI
                     KCFIPSGALSWRPLIFLAVVAGLFYQLGWRTIPVENPSYIKAETLDLADYVKTQTNPD
                     DVFLIVAEAEEAEWFPYLLQRTPAAASWGAEWSGNYAQQLEQVSKIAECQKTDSFACL
                     TESLQGLSAPPDLLITHLNTPIINQNLSDAPAWSVVYQNERYILWRHAD"
     gene            11909..13375
                     /gene="pyk"
                     /locus_tag="PKL11_02625"
     CDS             11909..13375
                     /gene="pyk"
                     /locus_tag="PKL11_02625"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNZ12523.1"
                     /translation="MQENAMTRRARIVATLGPSSKDEATIRALILAGLDVARLNFSHG
                     THADHAERIALLRRLSTELGRHVAILQDLQGPKLRVGTLPEGGIQLSAGQRVALTPVE
                     TEAKAKPGVTVIPMDVPNLAHSVQPGNRILLDDGHLEMQVTGVNGDSVEATVTLGGLL
                     SSHKGVNLPGTHLDIPGFTEKDREDLGFGLQQGVDMVAISFVRTAADVETVRKAIRQM
                     APKQDDTPIIAKLELPEAIENLHDIVHAADGVMVARGDLGVETSPAAVPIIQKEIIRM
                     ANRHAKMVITATQMLDSMIHNPRPTRAEASDVANAIFDGTDAVMLSGETASGSYPLES
                     VQMMATIVEEAEANFEQWGHCDTSTEAVTLDDAVSMTRAARELAHDRNVAAIAVFTQS
                     GKTALLMSKARPGVPVLAFTPHDRTCQRMNIYWNVMPIQVPFAFTVEEMLKIVEQTIL
                     TQTDIKPGQQVVVITGFPVGEFRTPNLALLHTIGSTGQ"
     gene            13581..14621
                     /gene="ord"
                     /locus_tag="PKL11_02630"
     CDS             13581..14621
                     /gene="ord"
                     /locus_tag="PKL11_02630"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HNZ12524.1"
                     /translation="MEPIRVLQWGLGAMGSGMVRLMLEKSGIKVVAVIDSRPDYVGKD
                     LGDVLGIGKKLGVIITDKPADVLKKEAVDVAVLATTSWVSEQMGDLRKVITAGINCIS
                     IAEEMADPYAQSPALAAELDQLAKKHGISVLGTGVNPGFVLDLLVVLLSGGNHRVERI
                     EASRINDLSPYGPTVMRTQGVGTTPEAFRAGVADGSIVGHVGFPESIHMISDALGLGV
                     DRIEQSREPIISKVRRETPHVVVEPGMVAGCAHIGVGYCGDKEVIRLIHPQQIHPQLE
                     NQDTGDYINIYGLPEIHMAIKPEIAGGKATMGIAVNMIPHVFAATPGLKRMIDLPVPA
                     ALMGESAYTRHV"
     gene            14736..15020
                     /gene="ortA"
                     /locus_tag="PKL11_02635"
     CDS             14736..15020
                     /gene="ortA"
                     /locus_tag="PKL11_02635"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HNZ12525.1"
                     /translation="MSKIAKGTWVEIEQVILTPEERATNLPEDTKKTPYVLRVSGFLT
                     AEAELGKEAQIRTIIGREFTGLLKTVNPSYSHSFGETVPELLTIGTEMES"
     gene            15017..16432
                     /gene="ortB"
                     /locus_tag="PKL11_02640"
     CDS             15017..16432
                     /gene="ortB"
                     /locus_tag="PKL11_02640"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HNZ12526.1"
                     /translation="MSGRDTSYAAVMARKNEIMKASLGIDYEEFIQSPIAFDYDRMMR
                     ETGFSLEDIVRIQSETKVGNTPLYELRRLTEAVRRISPPGKGARILLKDEAANASGSF
                     KARRASISAYQAALKGYAGMIAATSGNYGAAVASQAAQRGLKCIIVQEVYDSRGVGQP
                     EIVEKGRACEAYGAEVIKLTVGPELFYMLLRTLEETGFFNASLYTPFGIRGVETLGVE
                     IAEQVRSRYGKDPDAVVITHAGGGNLTGTARGLQIANSSQTRIVACSVDLSGLHMASD
                     HDFNRKSFTTGHTGFGVPFATWPDRVDVPRNAARSLRYMDEYHLVTQGEVFYITELLT
                     KLEGIERGPAGNTSLTAAVTLARQMDADQIVVVQETEYTGAGKHHNSQLSFARQNGIE
                     VRRGDPRENVPGKSIVIPERLDQVWGKEQDMDRLRISYLKNAARALPVAQWSENDICF
                     LAADVKKDAAWVQQQAAQLEA"
     gene            16474..16824
                     /locus_tag="PKL11_02645"
     CDS             16474..16824
                     /locus_tag="PKL11_02645"
                     /inference="COORDINATES: protein motif:HMM:NF027866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HNZ12527.1"
                     /translation="MNDERTAKFEKRRAELHKLSDAELKARFWELCDQVVEPIVDMAR
                     THTSPSIERSVLLRMGIDSVTSHGVVDRVYEAGLLGKGAGHVVLKVAQKLGKDVRAAA
                     AAIIEDKEILKGLF"
     gene            16840..19050
                     /gene="oraE"
                     /locus_tag="PKL11_02650"
     CDS             16840..19050
                     /gene="oraE"
                     /locus_tag="PKL11_02650"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF040743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HNZ12528.1"
                     /translation="MTTQQLDPNKKLNIEQILQDLEHYHPRRKGWTWRRVPAGGVDMG
                     PFHYRDMSEPLKRSIGLPASKYFDHIDPQPSCVVTTEIASGHFEDDIRRMRMAAWHGA
                     DHIMVIRTMGQSHIDGLLEGTPEGIGGVPITRKQIRATRKALDMIEDEVGRPINFHSY
                     VSGVAGPEVAVLFAEEGINGAHQDPQYNVLYRNVNMMRSFVDAAEAKKVMADACIFQI
                     DGAHNANATAKAGWLVMPELMVQHGINCAFSVAAGMVPELVGLSTVPPNAPPAPKLWY
                     DLPYAVALRDFFSAFKMRAQQNTRYIEADIEEAIRTHTVDTLISMLTSADIQSTITPD
                     EGRNVPWHYNNIRGVQTVKQTWAALDGIKELVSLNHEGPLGQMVRDIKERAVAFLEEI
                     IEIGGYFAAVEKGFFVDSAKYPERNGDGIARDSKGGIGANTIVARDPDYYAPVCSHFG
                     DNHVPAGVEHACDPIGGCTLCDPEKIVFVDELDPEDNCNKRLERTQPYRTGTAIKPEA
                     EWAGDGTVLLQFFVPLPEDVAAVYGVEMAKKMGLVDPEVVNITPMHPVEGCFLEVKGK
                     VDFDIDRTTLRVPEKEIILPEDELVAAVRQVGLRVVAGTVGEDEHSVGLREILDIKHG
                     GIEKYGVKYHYLGTSVPLGKLVDAAIETGAQAILISTIISHNDVHRTQMRKLHELCVE
                     KGIRDNIILIAGGTQVSREMAAETGLDATFGRGTKGIHVVNAMVKILRARGLVK"
     gene            19155..20495
                     /locus_tag="PKL11_02655"
     CDS             19155..20495
                     /locus_tag="PKL11_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNZ12529.1"
                     /translation="MTQQILDVLTIEVGSTITKVNGFRRLPEGGFEHGAQGFAPTSVA
                     AGDVGIGVEQAIIDLEARHGKLGSRPETFVNSSAAGGLRMTVHGLTYSMTARAAREAA
                     LGAGGIVKMVTAGTLSDFDLDELREINPNIILLAGGVDFGEKDIVLQNAEKLAGLQLG
                     VPVVYAGNAAIRKQVGKIFEGHGIKALIAPNVFPDVDVLNIAPLRKIIHEVFNQHIIH
                     APGMARFAELTHWGILPTPGAVLRGAELFAEAMGDCLIFDVGGATTDVHSVTDGSLEW
                     TAKLTEPEPYAKRTVEGDLGVFINAHNIIEMEGDPAWEARLEDLRAIPATEREKELTH
                     WLCERAVHTGARRHAGVVTDLYTPMGKKQIVKGKDLSAVQWVVGTGGALTRIEGGEGI
                     LRSICTGPGKYLLPPPDARILMDRDYRFSALGTLAQAYPEDVRLTFERWANSNT"
     gene            20534..21613
                     /locus_tag="PKL11_02660"
     CDS             20534..21613
                     /locus_tag="PKL11_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HNZ12530.1"
                     /translation="MYIRTPRLEIYPHRIEENARAVVGLCHKAGIQTACVTKVTSAHP
                     AVVTALVAGGADMIADSRILNLQSMVNTGLSVPLMLLRIPAPSRAADVVRCADYSLNS
                     SVETIELLSEAAQFLKVKHHVVIMVDLGDLREGVWPDRAAEVVCEAAKLPNIEVAGLG
                     CNLACYGGVIPSVENMQRLIEIREICRKASGLDLNLLSGGNSANLPLLLDGTLPKEIN
                     HLRIGEAIVLGRNVIDRSPLPGTRQDTFKIYAEVVEVERKPSVPIGNRGQDAFGGSPE
                     YVDRGIRLRAICNIGRQDVVVDGITPEDPGIIVLGGSSDHLILDVEEAETLVELGAEV
                     AFYPNYGALLAASTSPYMQKVVVRE"
     gene            21617..22741
                     /gene="alr"
                     /locus_tag="PKL11_02665"
     CDS             21617..22741
                     /gene="alr"
                     /locus_tag="PKL11_02665"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNZ12531.1"
                     /translation="MLYQTHARIHLNHIRFNIEGIRKAVGAERKILIAVKANAYGHGA
                     VAVSRMAEQIGVDWLGVATVPEGMQLREAGIGLPILKLSPAFPEEMEAAVRSRVTLAV
                     GEQSNIAALEEICANHGLTTHVHLKVDSGMGRIGVTVAEAPALAAFIERECPHLHLEG
                     VFTHLPVSDSADPTWTRAQIERFNGVVRAIETTVGHKIELAHCSNSGAVLGHAPGWMD
                     MVRPGIMIYGFYPDEGTPRAIPLKPGLSFLTRISFLKKVTAGTSIGYGRTWIAPEDTW
                     IATIPAGYADGFNRLFSNRGRVLINGRSYPIVGRICMDQSMVNLGPETTAKVGDEVVL
                     IGKSGLEEITVDEWARELKTITYEVTCQINSRVERIYDRY"
     gene            22828..23202
                     /locus_tag="PKL11_02670"
     CDS             22828..23202
                     /locus_tag="PKL11_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNZ12532.1"
                     /translation="MNTALWIIQIILSIKLMTTAYSHGLRQDQATMREARLKLGRPGR
                     ALLYATAIGTTAGALGLILPGVIGTAGWMTPTAAAWMSAMLLGSILCHVRSREKPKIF
                     VSIVLFVFAALVAYGRWVVTPL"
     gene            23221..23568
                     /locus_tag="PKL11_02675"
     CDS             23221..23568
                     /locus_tag="PKL11_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12533.1"
                     /translation="MTEQDEIEKLKEWQEHQFNSGYWVGRLPQDFPLKRSWKTYIIVL
                     IQFIFILVGTGVLILAYILTRDLNTLIAALFLIGLTAVTYLLVKRVKPLKDGKPGQEG
                     IEAGRRSENQEEK"
     gene            23636..24232
                     /locus_tag="PKL11_02680"
     CDS             23636..24232
                     /locus_tag="PKL11_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12534.1"
                     /translation="MTTDYALQVEVYPATAERWPDLEQILSQRRATSSCWCMFWRLRR
                     TDFSKLTDDEKKAALKAHTADRLAPGVLAYVDGKVIGWCSVGPRENFVALENSRILKR
                     VDDTPVWSIVCFFVDTPFRKKGVMAELLRGAVRYAEQNGARVIESYPLEMQSDKLAGQ
                     KLHGFSGYMGIASVFRAAGFVKVSDASETQVIMRYTVA"
     gene            24239..24787
                     /locus_tag="PKL11_02685"
     CDS             24239..24787
                     /locus_tag="PKL11_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12535.1"
                     /translation="METDDILQVFGLDAILTEADDPALWATALRQYWPRPNDPKCMVE
                     ALRFEIGRRLADGEQPQGEDNERDATMRGMVDIQKWGFERMDAERLQVKPRPAGEVPP
                     VILPGFGMIGLMSGQVPPTPPAQVVFIYLGISRPSRAADEAVVFSGFSAPGIGEGNAY
                     LWRCDETGQWVMTDQRVAWWIT"
     gene            24863..25387
                     /locus_tag="PKL11_02690"
     CDS             24863..25387
                     /locus_tag="PKL11_02690"
                     /inference="COORDINATES: protein motif:HMM:NF022521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 family protein"
                     /protein_id="HNZ12536.1"
                     /translation="MNQYPPLAKKLFAAQIRFAGIMSVVALIMGLVYREISRGFFDGL
                     SYEQQLLYGHNMSLVHGHTFLLGAVIPMVLAVLTLLTLPNLPEKRLKNMNVRFTAYMI
                     AASVALALMVYKGLAFIISAGQPLEAIDAGLFFGSGLLRGILFGLSHVVIFWAVGEYA
                     VGAIRAAGASPQKA"
     gene            25500..26738
                     /locus_tag="PKL11_02695"
     CDS             25500..26738
                     /locus_tag="PKL11_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12537.1"
                     /translation="MALQKTGKKTSKIGLILLAFVAFIALGMPDGLLGVGWPSIRAGF
                     GIPLDSLGMLLIASMTGYLASSFLSGKLIARMGVGGLLAASCAATGIGLIGYTLVPAW
                     GMMVALGVIAGLGAGAIDAGLNTYVAANFGEGLMQWLHASYGIGVTLGPIIMTLALNA
                     LNSWRVGYAIVGGFQLALAACFVLTLPMWTQKAHPAEAEKPQRLTDYKTSLRETLRQP
                     QVWLSISLFFLYTGGEVSLGTWAYTLLTESRGIDPQAAGLWAGSYWLMFTIGRVLAGL
                     YAKRIKGKLLVQISLLGALLGAGLVWWNLSEAVSLIGVALIGFAIAPIFPAMVSGTSQ
                     RVGDRFAANTIGMQMGAASLGASLIPGLVGVLAKNISLEVIPVCLFVLFGALLGAYLV
                     ATRKREAKAVREEHPAAAEM"
     gene            26765..27307
                     /locus_tag="PKL11_02700"
     CDS             26765..27307
                     /locus_tag="PKL11_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12538.1"
                     /translation="MIQTQRLIIRHFRAEDFSGLFDYLSNPEVYRFEPGDSITLEEAK
                     TLAAQRAESSEFWAVALKETDKIIGHLYFHQIEPQALKTWELGYIFNPAYQHQGYAVE
                     AAGALVRYGFAKWEIHRVVAYCNPKNTASWKLMERIGMRREGYLRKNIFFQTDVVDEP
                     IWLDTLAYAILEEDLAPAEV"
     gene            27350..27835
                     /locus_tag="PKL11_02705"
     CDS             27350..27835
                     /locus_tag="PKL11_02705"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNZ12539.1"
                     /translation="MITIAEFSKMLANENEIVHEQTRELSNADALIQPQPSGNCLNWV
                     LGHMLDNQILVLKTLGGSSPIERTETERYRRDSESIRAEGPGVWTLEQLLAGHDQLLA
                     ALTARLGELTETDLDEEIQLGERKVKRGWWVFFLHFHYTYHLGQLEYLRQLAGKTDKV
                     I"
     gene            28012..28203
                     /locus_tag="PKL11_02710"
     CDS             28012..28203
                     /locus_tag="PKL11_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12540.1"
                     /translation="MSAATRARLARHQSDRSRTIYVQVVDDNGYDLPPEVVEQKMKEA
                     QLQAGPRDTVIAVRHQVNQ"
     gene            28200..28598
                     /locus_tag="PKL11_02715"
     CDS             28200..28598
                     /locus_tag="PKL11_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ12541.1"
                     /translation="MTLTMGAVTLVVRDYDEAIAYFTNALRFTLLEDTPLGNGKRWVR
                     AAPGGSNGFALLLARAVTPEQESRIGNQTGGRVFLFLYTDHFEDDYRHMRASGVEFTE
                     EPRQEAFGRVVVFRDLYGNKWDLIESLTGG"
     gene            28671..29084
                     /locus_tag="PKL11_02720"
     CDS             28671..29084
                     /locus_tag="PKL11_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12542.1"
                     /translation="MKTEKNIVLWGQGLVILNAVIWMAVGIVWLLRQHSPAVMPLWVV
                     WGMGIAMIGYGGALLFLGVQLRRQKQGIYLAAVALISIAIIAGLFDDLGLADLLGLLP
                     GLAALIYLWIYKNVLSGAGPAPKGNSHDAIRVHQG"
     gene            29056..30078
                     /locus_tag="PKL11_02725"
     CDS             29056..30078
                     /locus_tag="PKL11_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12543.1"
                     /translation="MTRLESIKGNVLEWLLEADSPGVRYLVLRDLAGRSENDPELKAA
                     CRAAHQQGPIAKVLDAMSPEGYWVQPGAGYAPKYRGSVWAMILLAQLGARIDQDERIA
                     TACQYMMEHALARQGQFSYNGAPSGTFDCLQGNLCWALQELGCLDSRLDGAFEWMARS
                     QTGNGIAPMTDKKAEVRFYTYKCGPNFECGANNRQPCGWGAVKVMLAFSKLPPEKRTA
                     EIKQAIQQGVDFLFSGDPAMANYPTPEGQAPSHNWWKFGFPVFYVTDLLQNVEALAGL
                     GYGNDPRLSRALEIIRDKQDADGRWAMEYDYTSKTWVNFGPKKRPNKWVTLRALRVLK
                     IAEQKL"
     gene            complement(30134..33262)
                     /locus_tag="PKL11_02730"
     CDS             complement(30134..33262)
                     /locus_tag="PKL11_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ12544.1"
                     /translation="MTEIQTLLHAIAALEAQRSSLGDDVVNAALAPMRQKLALLQGQA
                     QLAENQRKQITVLFANITGLAAPSENLDIEESRDMIQDVWKRLDPIIISHGGAIDKHI
                     GDTVMALWGTSVVREDDPERAIKAALAMQAALTAFCKERHLGLSIRIGLNTGPVLLGE
                     VGSTHEFTAMGDTVNLASRLESAAPVDGILIAHNTYRHVSGMFDVQVQPPLTVKGKAE
                     PVQTYLVLRAKQRQFWVATRGVEGIRTHMIGRDVELAYLQSTLGTVAEQRKLQMVTLV
                     AEAGLGKSRLLREFDAWLETYQKSCVVIRGRASQEMQSVPYALLRDIFSFRFQIQDRD
                     PLKTVQEKMETGIGAGASVSGAAWPASESPMRAHIIGHWIGFDLSESPYLTEILEDAQ
                     QIRDRALIYLVDYFKSLTESSTIVILLEDIHWADDSSLDVFEHLGHMLSRHPVLIITA
                     ARPSLFDRRPSWASGQPVQNRLNLSPLPEQDSQRLVDDILQKVESIPPMLRSLMINNA
                     EGNPFYMEELLKMLIEDGVVTKGIEHWQVNPDRLAVVRVPSTLTAVLQARFDLLTALE
                     KNTLQCASVFGRTFWEKAVDFIAKGAARLDTERSLSALCSREMIFQSDTPTFAAMAEY
                     IFQHTLLRSTVYESILKRDRPAYHARAADWLISHSAERESEFIGQIAEHFERAGNAEK
                     AVKYLARAAVGALRTYAYRESADYFSRALNLLPSGNRDRIPLLVQFGEALWYLGSYEQ
                     AQNLLHEGLSLGKAHGEEGWCADALIHLAGIARKQGNWPQAQTFLNESLEIARRLGDQ
                     SRIAHALHTLGWLNIRQGAYAEARAFLLESRAIYAALDDRHGLADSLNGLGTVALNLG
                     EYEQTRQLYQESLALFHAVSDRPGESAALVNLGEAARRQGDYAAARQYYEMALKIDHE
                     MGDELLAAITLGNLGHTALACHDYPMAEAYYRKGLQTAAAINDIPDILDILSGLSGVL
                     THTNRSEQALMVLSVVLNHPGLEDESRTIAEQALADLQHTQPQAVHAAGLQAGREYNL
                     EEVVTEILASFE"
     gene            33397..33903
                     /locus_tag="PKL11_02735"
     CDS             33397..33903
                     /locus_tag="PKL11_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12545.1"
                     /translation="MVKVREAELKDCHGIARVQVDSYRTAYAGLMPEPYLAQFSYEEQ
                     EQDWVGLLAADSADILLVAESEEGVIVGYTLARAEAGIYPGYDAEIIALHVRKSVQRQ
                     GVGRALLRGAVEKLIARGCQSVMLWTLKGNPVRRWYEKLGGKYLGEKTWPLDDFEVHE
                     VAYGWDIG"
BASE COUNT         7672 a         8614 c        10653 g         7116 t
ORIGIN      
        1 aaggataagc gcgggcagat taagcccatt ctgacgcggc tgcagcgaga atttaatctc
       61 tctacggcag aggtggggct gcaggatgaa tggcaggaaa cgatcctggc ctgtgcaatc
      121 gtcagcaccg ataaggtgca caatcaacag gtgctgcagc aggtggtgga ttatacggcc
      181 cggcactggc ccgaggcaga gatcttaaaa catacgattg aaacatggta aggaataagg
      241 aggtattatg gatctgggat tgaaaaacaa gattgccctg gtcaccggcg caagccgagg
      301 actgggtttt gccaccgccc gcctgctggc gcaggagggt gcgcgggtcg ccatcaatgg
      361 gcgcaacgca gaggcgctgg agaaagctgc ggcgcagatc gccgcagaaa ccggcgccga
      421 gacgctggcc ctggcagggg atgtggtgga cccggctacc ccgccgcggt tgatggagga
      481 aatcaaggca gcctggggcg ggctggatct gctggtagcc aatgccggcg gaccaccggc
      541 aggcgctttt gaaagctttg atgatgaagt ctggcaaaag gcggtggagc tttctttttt
      601 gagccaggtg cgcttgatta gggcggcgct gccgctgttg aagcaatcag actgcgccag
      661 cgtgctgacg attacgtctt attcggttaa acaaccaatt cccaacctgg tgctgtccaa
      721 cagtatacgg gctgcaaccg tgggactgac caaaagtctg gcgctggaac tgggtgggca
      781 gggcatccgc ttcaactcta ttctgccggg atggaccgag acagagcgcg tggtgaacct
      841 gatgtcggcg cgggcgcaga gcagcgggcg cagcattgct gaagagaccg cccggcaggc
      901 ggctgacagc ccgctggggc gcatggggca gcctgaagaa tttgggcgcg cggctgtatt
      961 tctgctgtcg ccagccgcca gctatatcac gggtgtgatg ctgaccgtgg atggcgggat
     1021 gtacaaaggc acgctttagc gcgcgggaac gcccagcagg cccaacattc cggggccggt
     1081 atgaaccgac aggcccgggg taaattcaga gatcatgatt tcggccgggc aggggagcgc
     1141 agtgcgcacc tgctcggcga agtcctgcgc ctcgtccagg cagtcggtat gcacaatgcc
     1201 cagtttttcc agtgggcggc cattgagggc gttgctcatc aattcaatca tccgagccag
     1261 ggctttttta tgggtgcgcg tgcgctccag catttccagg cggccatcgt taagcgtcag
     1321 caccgggcga atatccagca gtccggccat gttggcctgg aatttggtca ggcggccccc
     1381 taaaacaagg aatttgagcg tggataaagc gccaaacaat aaagcgcgct ggccaatgga
     1441 ttggctgact tccaaagcct gagtcattga gccgccagag tgaatcacat ctgcagcggc
     1501 aagcgccata aagccctgcc ccagcgaaac gctgttggtg tcaattactt caatttcgcg
     1561 gtctgggagc atctcgcggg ccagaactgc cgcaccgtac gtggcgctca ttttggcgct
     1621 gatgcagtag cagataatcg catcggcgcc accctcaaaa gcctgctgat aagcgcgcaa
     1681 gaattgttcg ggagaaggcg cggccgtagt gggcagtttg ccggtgcggt tgacgcgctc
     1741 aaacaggcgg gcatcgtcaa tatccacgcc ggtggtaaag gactcatcgc caaaattgat
     1801 gccaattggc accaattgaa tattatatga gtgggcaaca tcggccgaga gactggaatc
     1861 agtatcggta ataatggcaa tttttgacat tgggggatca ctcctcaggg gatagtctaa
     1921 ttgtaaccga tagactccaa aatcgggtac aattgccaga tatttgtgag gatcaatgac
     1981 taaaagactt tcggaatctg ctgaaattat acaagaaaag ctggaaagga ttctgccaac
     2041 cgtcaccaaa cctgggcgtt acactggcgg ggaatacaat caggtggtca aagactggca
     2101 ggcggttcag acccgcgtgg cgctggcctt tcccgatatt tatgacctgg gactgcccaa
     2161 cctggggctg gctatttttt atgaagccct gaatgaccgg cccgatgtgt tggctgagcg
     2221 ggtgtacctg ccgtggctgg acatggaagc ggccctgcgc agcgccgggg tgccgctcta
     2281 ttcacttgaa acgcaccatg cgattgcaga ttttgacatt ctgggcatta ccctgccgta
     2341 cgaaacgctc tataccaata cactcaatct gctcgacctg gccgggattc cgctgttgag
     2401 taaagagcgc acggcgcagc atccgctggt gattgccggt ggacatgcct gctacaaccc
     2461 ggaaccgatg gcggattata ttgacgcctt tgccatcggg gatggcgaag aggtttttca
     2521 cgacattgta aatgtacaac aggcctgggc taaaagcggc gcagatcggg ccgccctgct
     2581 gcgggctctg gcgcagattc cgggggtgta tgtgccggcg ctgtatgcgc cgcgctatca
     2641 cgcggatggc acgctggcag gagtagaacc ccttgacaac agcgctcccc ggcaggtgct
     2701 taaacgcgtg gtggggcagc tgccgccggc gccgacccgg tttattgtgc cttccattga
     2761 tgtcgtgcat aaccgcgttt cggtggagat tatgcgcggg tgtacgcgcg gctgccgttt
     2821 ctgccatgcg ggcatggtga accgccccgt gcgggagcgc agtgtggcag aggtggtgca
     2881 gaccattcaa acggccgtta aacagacggg atttgaggag atcgccctgc tctcgctctc
     2941 ttcatctgat tactcccgga tcgtggaact ggtggaacag gtccgtgaaa aggtcacggg
     3001 gccgcatttg acggtttcac tgccatcgct gcggattgag tctttctcgg ttgaattgat
     3061 ggagcggctg cgcggcggac gccagggcag ctttacgctg gcgccagagg cggcaacaga
     3121 ccggatgcgt aatactatta ataaacccat tcaggaccag cagctcctgg aaaccgccgc
     3181 tgaactttac gggcgcggct ggcccacgct gaagctgtat ttcatgatcg ggcatccatc
     3241 agagacactg gaagatgtgc aggcgattgc cgacctgtgc ctggaagtga tggccgtggg
     3301 gcgaaaggca atcgggcggc gggccagcct gcatgtcggg gtgagtacat ttgtgcccaa
     3361 gccacatacc cccttccaat gggtggcctg cgacgcgccg gagatgattg aagccaaaat
     3421 ccaactgctg cggcgagcgc tgcgcagtcc agggctcaag atgaactgga ccgacccccg
     3481 cgagacgctg ctggaagcct ggttgagccg gggcgaccgc cggctgggag aggtgatctg
     3541 gcgggcgtgg cagcacggcg ccaagtttga cgcctggcaa gaccagttca attttgaggc
     3601 gtggcaaacc gcctttgaag aaagcgggtt gaacccggct gactatacgc atcgtgaccg
     3661 cgcggaagat gaggtgtttg cctgggacca catcagcccg ggggtcagca aggcttacct
     3721 gctggatgaa tatcacgcca gcctggaagg gcggctgacc ggcgactgcc gggagcagtg
     3781 ctacgcctgc ggcatcttgc cgcaatttgc cgccatgcgc cgccaactgc ccaaatccgc
     3841 ctggttctgt ccgggcggcg atcccgccaa agcctgaggc tgccatgaaa agaattcgaa
     3901 tccattacgc caagactgaa ggactgcgct acaccagcaa cctggatatg caaaaggttt
     3961 gggaacgcgc gctgcggcgc gccgggctgc cgctgtacta ctcacagggg tttcaccccc
     4021 agccccgcat tcaacaagcc tgtcccctgc cgctggggct gctcagccgc tgcgagattg
     4081 tggatgtctg gctggaagat gacacctgca cattggaagg cacgcttaaa gcactgcagc
     4141 cggctctgcc caccgggatt gaactgctca aactggaaga gattgatccg gcggcgccgg
     4201 cacttcaaaa gctggctggc agcgctgatt acgatatcac cctgctggac ccctttccgg
     4261 ttgacgaact ggagcgccgc acggctgctc tgctgggcgc cgaggcgtta attcgtgagc
     4321 ggcgcggcaa gaattatgat ctgcgcccac tggtggaaga cctggctgtt gttccaggcg
     4381 aaccgctgcg gctgagcgcc cgcctgtcgg tgcgcgatgg cgccaccggc agacctgatg
     4441 aactgctggc ggcactgggc ctggacccct ttggcgcgcg catcgtccgg gtacggttga
     4501 tcctggctga ggcctgagat tattactacc ctggtttcat ctgcaccaac gaaaatagaa
     4561 gaagctggat ccacatgaaa tttgctgtca attattcaca cgccgcggcc gatctactgg
     4621 aggccgggca aattgatttt gacctgttca agttccccga ctggcccgat tttgtgcctc
     4681 agaacgggtt gatacgcaag ccctatgttc attacaccct ggcggctgga aatggcagcc
     4741 aggcggcagt tgactggcag atgattgccg ggctgcgggc gcgtacggct tcgccgctgg
     4801 tcaatacgca cctgatggcg cccgccgggc ttgagccgga tgacccggcc caggttgagg
     4861 cggccattca ggggatggtt gcagacctgc aagatctggt ggcccggttt ggggccgaaa
     4921 acgtggttgt ggagaacatg ccgttttacg ttcaaaatcg tcatatgcgg gtggccattg
     4981 aaccagcgac gattcagcgc gtggtgggcg aaagcggctg taagttcctg ctggataccg
     5041 cccatgcccg catcacctct gcggcgctgg ggattgatgt ctatgattac atcaatgcgc
     5101 tgccagtgaa ccggctggga gagctgcatg tcaccggggt tggctggcac aatggtgaac
     5161 gcattgatca cctggagttg ctggccgaag actgggaagt gtttgactgg gtgatggccc
     5221 atatcgcggc cgggcactgg ccacaaccga aagtcgtgtc gtttgaatac ggcggggcgg
     5281 gcgaggtttt ccgctggcgc acggaagtgc gggtactggc tgagcaaacc ccgcgattgt
     5341 ttgccacggt tcaggccgcc cggcacaatt gatttaccca tgacactaga gacgatcatc
     5401 cgccagtgca gcgacccaac ctgccgcttc cgctacccgc aggcggcggg gccagcgcgc
     5461 ccggggcacc cctgcccgcg ctgtggtgcg ccgaccgaaa ttgcaccgct gccactgctg
     5521 gagccggacg acaacccgcg cagcctggaa gccagcggcc ccccggtgga ggccctgctg
     5581 gataacgtcc gcagtacgtt caatgtgggg gcgatgttcc gcacggcaga tggcgccgga
     5641 gtgagccatt tgcatctttg cggggtaacc cccaccccgg agaatccgcg cgtgggcaag
     5701 acggcgctgg gggcagaata tgcggtgccg tggacgtggc atccgaatgc gctggatgcg
     5761 gcgctggctg gcaaggcccg cgggctgcgg ctgtgggcgc tggaggtggg accccatgcg
     5821 ttggcactgc cagaggtccg gtcggttatc tctgatgagc cgatactgct ggtggctggc
     5881 ggcgaggtga gcggagtgga cccggcgatt ctggcgctgt gtgagcgcac attgtggatc
     5941 ccaatgcagg gcttcaagcg gacgttgaat gtggcgattg cgtttggcat tgcagtgtat
     6001 gggctgcggt ttgcgggttg aacaaaccca ttgagatttg acaggccaaa ctcaaagcat
     6061 tagaatcgca agcgagagat tatctgcgag gtacctatgc caatttatga gtatgtatgc
     6121 gaagattgtg ggcgcaaatt tgatgcgatg cgctcgatga aagatgccga tgcgccaatt
     6181 gcctgcaagg gctgccaaag ccagcatacg cggcgggcgc tatcgctgtg ctgtgcacac
     6241 agcgatggac aggtggtcag cggcgggggc gggggctgcg ggggatgcag cggaggctcg
     6301 tgtggttcct gcggtcatgc gcattaggtg atgaaaaagt ctgtctgggc cgggaaactt
     6361 acgcttatta cggggggctc cagcgggatt gggctggcac tggctaaaaa gctggctgcc
     6421 gccggggcgc aggtacacct gctggcgcgg cggctggatc aactggaatc tgccaaggct
     6481 gaggttgagg tagtccgtgc tgaccctggt caaaccgttg ggatactggc gggcaatgtg
     6541 gcggaggcag aagcaattac cgagcgcctg cacgagtttg aacagacggt tggtacaccc
     6601 gacctgctga tcaattcagc ggggattacg cacccagcag aatttcaaga caccgacctg
     6661 gataccttcc gccgcctgat ggaagtcaat tacctgggga cggtgcatgt cacacgggcg
     6721 ttcatcaaag ggatgctggc gcgcggtgcg gggcagatca tcaatatttc ctcggctgcg
     6781 gggtttgtgg gcacgtatgg ctacacggct tacagcggat cgaagtttgc ggtgcggggg
     6841 ttcaccgacg cgctgcgagc agaggtcaaa ccgcgcgggt tgaaggtgtc aatcgttttt
     6901 ccaccggaca ccgacacgcc acaactggcc tgggaaacgc cgctgaaacc cgccgtgacg
     6961 cgggaattct caaaggctgc ccaggtactt tcagccgata aggtggcaga aattaccctg
     7021 agagcggccg ggcgcgggca gtacatcatt gttcccggat ttgacaccca tttgttctat
     7081 cacttgaata attttctggg gccgctggcc ttcaacgtga ttgacctgat ggtggcagac
     7141 gccatcaaaa aggcgaaaaa atagcgtacg gtctgtgatt ctcaaaccca aggcagaggg
     7201 cgggctgtgg tggggcggct ggggctcaaa atagacggca cgcagcgaca ttcttcatgg
     7261 taagggctta tttaggtcac tagccatttg gaatgggtgt gatatacttt tgccggtgtc
     7321 caactttttg tatttcagtg aggagtatca aatgagaaaa gactttttgg ccatttcgga
     7381 ttataccccg gctgagctgc aggccatgct cgacctggct gtgcgcctga agaaggagct
     7441 gcgctccggt ggcaatgccc ccctgctgca aggcaaagtg ctgggcatga tcttccagaa
     7501 acccagcctg cgcacccgcg tgagctttga catggcgatg cgccagcttg gcggcgatgc
     7561 actgtacctc tcgccgaatg aaatcggcct gggccagcgt gaatcgattg cggatgtggc
     7621 gcgtgtgctt tcgggctatg tggatgccct gatggcgcgc gtttttgcgc acgatcatgt
     7681 gctggaactg gcaaagtggg ccagcattcc ggtcatcaat ggcctgagcg attacaatca
     7741 tccctgccag ggtatggccg atgcgctgac cattattgaa aaatttggca gcctgaaggg
     7801 tctgaatgtg acctatgtag gcgacggcaa caatgtggct atttcactga tgcatatttg
     7861 cgcaaaactg ggcgccaact tcatcattgc caaccccgaa gattacgata ttctgcccgc
     7921 ggccgttgaa attgcgcgcg gttttgccgc caccagcggc agcaaactga ccttcctgcg
     7981 cgatccgcat gaagccgtgc gcggcgcgca cgtgatctac accgacacct ggaccagcat
     8041 gggccaggaa gctgaaggca agaagcgcga actggtcttc ccgccctacc aggtgaatga
     8101 aaaactggtc agcgaagctg acaaagacgt gattgtgctg cactgcctgc cggcgcaccg
     8161 caaccatgag atcaccgacg aagtagccga tggcccgcac tcgatgctct tcccgcaggc
     8221 ccacaaccgg ctgcacgccc agaaggctgt tctggtcacg ctgttcggca aagcctaatt
     8281 tcgttacgca tagactttaa cctcaaacaa cacctggaat ggaagccccg ttccaggtgt
     8341 tgttgaaaaa tcaacgcctg gagaaaaaag aaatggactg gactcaacac taccaatcgc
     8401 ataagatgag cgcggcagat gccgtgcggc tgataaaatc ggggcagcgg gtctttatga
     8461 ccggtaactg ctcggtgcca cacgcgctgc tgggcgcatt ggtcgaatat gctccagaac
     8521 tgcatgatgt ggaaatctgc caggcgctga cagtggctaa cggggattat gtgaccccgg
     8581 cgatggaagg gcacctgcgg gtcaattcgc tgttcatcag tcacaacgtc cgcaaggcca
     8641 tctgggaggg gcgggcagac tttacgccgg tgctgctttc ggagctgccg ctgctgttca
     8701 aacgggggat tttgcccgta gatgtggctt tgatccatgt ttcaacccca gacgaagatg
     8761 gttactgcac gctgggcgtg gaggcgggcc tctccattac cgccgccgag gcagccaagc
     8821 tccggattgc ccaggtgaac cggcagatgc cgcggctgat tggcgatacg cggatgcaca
     8881 tctctaaaat cgatgcgatt gtcgaggtgg atgagcctct ttcagagctg gcaatgggcg
     8941 aagaaggtga tcaggcgctg gtgcaaaaaa ttgcggggta cattgccgag cggattccgg
     9001 atggcgccac catgcagttg ggcattggag caatcccgga tgcggtgcta aagtacctgt
     9061 tccataagaa agacctgggg gtgcactctg agctgttctc agatggcgtg attgatctgg
     9121 ttgaagccgg ggttctcacc ggggcacgca agaccctgca cacgggcaag attacagccg
     9181 ggttcctgct gggtacgcgg cggttgtacc agtgggccga taacaacccg ctggttgaaa
     9241 ttcagcgcac cgagcatgtg aacaacccgt ttatcatctc tcagaaccgc aacatggtgg
     9301 cgattaattc ggccatcgag gtggatttca ccgggcaggt ctgcgccgac agcattggca
     9361 cacgcttcta ctccggcgtg ggcgggcaga acgattttat ttacggtgca tcgctgtcgg
     9421 aaggcggctt gccgatcatt gcgctgccgg ctacgaccac cctgcgggat ggcacggttg
     9481 tcagccggat tacgccgatg ttgaagccgg gcgccgggat caccactggg cgcaaccatg
     9541 tgcattatgt ggctactgag tacggtatgg tggacctgta cggcaaatcc atccggcagc
     9601 ggacacaggc gttgatttcc attgcgcatc cagacttccg ggacgagctg acccggcagg
     9661 caaaagattt gaattacgtg taaacccaga atatatagct gattgaacgc aggctcagca
     9721 tgaatgggtc tgcgtttttt tatcaatccg catgtcgcca aaggatgtaa cgttcattct
     9781 gatatacaac agaccaggct ggggcatcag acagattctg gttaatgatc ggcgtgttta
     9841 aatgcgtgat caataaatcc ggaggggcgg ataagccctg taaggattcg gtcaggcagg
     9901 caaaggaatc tgttttttga cattcggcaa tttttgagac ctgttccaat tgttgggcat
     9961 agttgcccga ccattctgct ccccaactgg ctgcggcagg tgtgcgctgc agcaaatagg
    10021 ggaaccattc agcctcttcg gcctcggcca caattaagaa aacatcatcc gggttggtct
    10081 gggttttgac ataatcagcc agatccaacg tttcagcttt gatgtacgag ggattttcaa
    10141 ccgggatggt tcgccaacca agttggtaaa acagcccggc cacaacagcc aggaagatga
    10201 gcggccgcca ggataaagcg ccggatggga tgaaacattt gatgcgttca gccagagcgc
    10261 tggacagcat accgattaga accgcgccgg aagtgatcag atagcgatcg ccctcggaca
    10321 tcaacagcag gatcaaacca aaccagacgg gcagaaggct ggcccgattt atagcagcaa
    10381 aaatcaacgc caatacaatc aagccccaga caaattgcag tttataaata gcgtgcaatg
    10441 agctttcgaa ccagggcagc aggcgggctg gatcctggaa gatggatagg aaatagttat
    10501 tcccgtgaga ttgaaaagca cctgaaaaaa tctgccaacc gtgctgttga accattaccg
    10561 ttacccaggg agccaccagg gccagcacaa ccagagatgt gatcaaggcg gttgaatagg
    10621 ttggcttgcg gttaagatgc gtgagtgtgt aagccacgac aaacaagaaa gcaaattccg
    10681 catagcctaa atgggttagg acagtcagcg caaacattat gccggcaaac agtgcgggcc
    10741 tccgacggga aaactgcgct gaccgcagat agaaatacac accccagagt gcaaagagaa
    10801 aagccagtgc gcgcaccatg cccccggaag tgccatgaat atagaatatt cgctgtgaag
    10861 cagccacaac caaggccgca atgacagcag cgccgcggga gcgggtaagc tcaaacgtga
    10921 gcagcgccag cggtatgaga gccagccatg aaaacaaagg cggggcaaac cgcaggtagc
    10981 taaaatcgga gatgttgaat agagaggtga ccagccccat caggtaaaag ccaaccgggg
    11041 gataggcaaa gggcatccct ccagggccat aataagggac ggtttgcggc agtctaaaac
    11101 cggctgacac caattcatct gacatcaagg tatacaagcc agcataaccg gcggggtttt
    11161 catatttaag agccacccca aacaataacg gcagggccag ggccagcggc agaattaaaa
    11221 agcggtaacc agacatcgcc aggtagaaca agcgcggggt atcagcggca gcctgttgaa
    11281 tccgtttaat tcctgagcgg gcgagacctg tcagaactgc ccaggggcga tgacgtagaa
    11341 cgcagagatc ataccctgcg attccaagcg ccaccagcag ggctaaaatg agcagcgggc
    11401 gcagtcgagc aaagaaaaag cggttgggca gcagggtggt gaacgtcatt aacaacaaaa
    11461 aggccagggc cagcagcagc acaaaaaata gcgttgaaac gactggcgaa aactgaaaaa
    11521 gtttttccaa ttgctgagaa aaacgaccgc cccgagagat gccatataaa gctgccccgg
    11581 ccaacagacc aacggcgatc atggcaaaga tcatcagcca gcggttcagc gaatagcccc
    11641 acagccattg attctcaagg tcagagggag cgcttagcgt taacacaatg gtcccgattg
    11701 aacacatgaa tagataggtg aagtaaagag aaagaggttt aagcttgagc atttttggca
    11761 acatcacggg gtatccacta tttgagcgga ctggtctcct taatcaatat cggttgtatt
    11821 ttaacacgtt gtcaaaactg gtcggtaaaa cgtcacagtt ccatcagcaa atttgaggaa
    11881 aaatgtgatt taatagaact aagattgatt gcaggagaat gccatgacac gcagagccag
    11941 gattgttgcc acgctgggcc ccagcagcaa ggatgaagcg actattcgag cgttgatttt
    12001 ggccgggctg gatgtggccc gtttgaattt ttcacatggg acgcatgcag accatgctga
    12061 aaggattgcg ctgctgcggc ggctttcaac cgagctgggg cggcatgtgg caattctgca
    12121 ggatttgcaa gggcccaaac tgcgggtagg aaccctgccc gaaggcggga ttcaactctc
    12181 tgccgggcag cgggtggcat tgacgccggt ggaaaccgag gcgaaagcta aacccggtgt
    12241 cacggtgatc ccgatggatg tgcccaatct ggcacattcg gtgcagcccg gaaaccgaat
    12301 tttgctggat gacgggcacc tggaaatgca ggtgactggc gtgaacggtg actcggttga
    12361 ggcgacggtg acgctgggag gattgctcag ctcacacaag ggcgttaacc tgccggggac
    12421 tcacctggat attccgggct ttaccgaaaa ggaccgggaa gacctggggt ttgggctgca
    12481 gcaaggtgtg gatatggtgg cgatttcgtt tgtgcgcact gcggcagatg tggaaaccgt
    12541 gcgcaaggcc atccggcaga tggcgcccaa gcaggatgat acgccgataa ttgccaagct
    12601 ggaactgccc gaggcaattg aaaatctgca cgatattgtg cacgcagcag atggggtgat
    12661 ggtggcgcgc ggggacctgg gggtggaaac ttcgccggcg gctgtgccga ttatccaaaa
    12721 agaaattatt cgtatggcca accggcacgc caaaatggtg attactgcca cgcagatgct
    12781 ggattcgatg atccacaacc cccgccccac gcgcgcggag gcatcggatg tggcgaatgc
    12841 catttttgac ggcaccgatg cggtgatgct ttcgggcgag acggcgagcg gcagctaccc
    12901 gctggaaagc gtgcaaatga tggcgacgat tgtggaagaa gccgaagcaa attttgagca
    12961 gtgggggcac tgtgatacct ctaccgaggc ggtgacgctg gacgacgcgg tctcaatgac
    13021 ccgggcggcg cgcgaactgg cgcacgatcg aaatgtggca gcgattgcgg tcttcacgca
    13081 gagtggcaag acggccttat tgatgtctaa ggcgcggccg ggggtgccgg tgctggcctt
    13141 cacgccgcac gaccgcacct gccagcgaat gaatatttac tggaatgtca tgcctatcca
    13201 ggtgccgttt gcctttacgg tggaagagat gctcaagatc gttgagcaaa ccatcctgac
    13261 tcaaaccgac attaaaccgg ggcagcaggt ggtggtgatt acgggattcc cggtaggcga
    13321 attccgcacg ccaaatctgg cgctgctgca tactattggc agcacaggtc agtagccaca
    13381 gttccggcag acccatttca gcctgtatta cagagaatca gacccatttt gggtgtgatt
    13441 ctcttccctt ttatatagac gaacctccac acgtccatac caattgcggg agtccgtgat
    13501 aagataaggg tgcaaaattg ggcaaatgcg cggttttgcc agttcaacca cccaaagttc
    13561 tgatttgaaa ggaaaagtat atggagccaa ttcgtgttct tcagtggggc ctcggtgcaa
    13621 tgggcagcgg aatggtgcgc ctgatgctcg agaagtctgg cattaaggtt gttgccgtca
    13681 ttgacagccg ccccgattat gtgggtaaag acctgggcga tgtgctgggc attgggaaaa
    13741 agctcggcgt catcatcacc gacaagccag ccgatgtgct gaagaaagaa gctgtggatg
    13801 ttgccgtact ggcaacgact tcctgggtca gcgagcagat gggtgatctg cgaaaagtga
    13861 ttactgctgg catcaactgc atttcgattg ctgaagaaat ggccgatccg tatgcgcaaa
    13921 gcccagccct ggcggctgaa ctggatcaac ttgccaagaa gcacggcatc agcgtactgg
    13981 gaacgggtgt gaaccccggt tttgtgctgg acctgctggt tgtgctgctg agcggcggca
    14041 accatcgtgt ggaacggatt gaggcctcgc ggattaacga cctgagcccc tacggcccaa
    14101 ccgttatgcg gacgcagggc gtgggcacca ccccggaagc cttccgggca ggtgtggctg
    14161 atggctcgat tgtggggcat gtgggcttcc ccgagtccat ccatatgatc agcgatgcgc
    14221 tgggcctggg tgtggatcgg attgagcagt ctcgcgagcc gatcatcagc aaggtgcgcc
    14281 gtgagacacc gcatgtggtg gtggaacccg gcatggtggc cggctgcgcg catattggcg
    14341 tgggctactg cggcgataag gaagtcatcc ggctgatcca tccccagcaa atccatcccc
    14401 agttggaaaa ccaggacact ggcgattaca tcaatattta cggtctgccg gaaattcata
    14461 tggcgatcaa acccgaaatt gcgggcggta aagccaccat gggcattgcc gtcaacatga
    14521 ttccgcatgt ctttgcggcc actcccgggc tcaagcggat gattgacctg cccgtcccgg
    14581 cggcgttgat gggtgagagc gcctacaccc gacacgtcta gttccaaatc aaacccgggg
    14641 aacagtctgc ctgcacgtcc gcccatcccg aaagcatatc agggttttcg ggatgggtat
    14701 ttgcagggca agcctccggc caagcgaggt gaataatgag caagattgct aaaggtacct
    14761 gggtcgaaat agagcaggtg atcctgacgc ctgaagagcg tgccaccaac ctgccggaag
    14821 ataccaagaa gaccccctac gtgctgcggg tctcgggatt tttaactgcc gaggctgaat
    14881 tgggaaaaga agcccagatt cgcacgatta ttggacgaga atttacgggg ctgttgaaga
    14941 ccgtcaaccc cagttacagt cacagttttg gcgagaccgt gcccgaactg ctgaccattg
    15001 gcacggagat ggaatcatga gcgggcgtga tacctcttat gctgcggtga tggctcgtaa
    15061 aaacgagatt atgaaggcct cactggggat tgactacgaa gaattcatcc agagcccaat
    15121 cgcctttgat tatgaccgga tgatgcggga aaccggtttc agcctggaag acattgtgcg
    15181 catccagagc gagaccaagg ttggcaatac gccgctgtat gagctgcgcc gattgacgga
    15241 agctgtgcga cggatttctc cgccgggcaa aggcgcaagg attttattga aagacgaagc
    15301 cgccaatgcc tcggggagct tcaaggcgcg gcgcgcctcc atcagcgcgt accaggcggc
    15361 attgaagggt tatgccggga tgattgccgc aaccagcggc aactacggtg cggcggtagc
    15421 ctcacaggcg gctcagcgcg ggctgaagtg catcattgtt caggaagtgt atgacagccg
    15481 gggtgtgggc cagcctgaga ttgtcgaaaa agggcgcgcc tgcgaagcct atggggcgga
    15541 agtgatcaaa ttgactgtgg gacccgagtt gttctacatg ctgctgcgca cgctggaaga
    15601 aaccgggttc ttcaacgcca gcctgtacac cccctttggc attcgcgggg tggagacgct
    15661 gggggtggag attgcggagc aggtgcgctc acgctacggc aaagaccccg atgcggtggt
    15721 cattacccac gcgggcggcg gcaacctgac gggcactgca cgcggtctgc agattgccaa
    15781 cagcagccag acccggattg tggcctgctc ggtagatttg agcgggctgc atatggcctc
    15841 ggatcatgat ttcaaccgca agtcattcac caccgggcat actggttttg gcgtgccgtt
    15901 tgccacctgg cccgaccggg tggacgtgcc ccgcaatgcg gcgcgcagcc tgcggtatat
    15961 ggacgaatac cacctggtga cccagggcga ggtcttttac atcaccgaac tgctgaccaa
    16021 gctggaaggg attgaacgcg gcccggcagg caacaccagc ctgactgcgg cagtgacgct
    16081 ggcccgccag atggatgcgg accagatcgt ggttgtgcag gaaactgagt acaccggcgc
    16141 cgggaaacac cataacagcc agctttcgtt tgcacggcag aacggcattg aagtgcggcg
    16201 cggcgatccg cgagaaaacg ttccgggcaa atccatcgtc atccccgagc ggctggacca
    16261 ggtttggggc aaagagcagg atatggatcg gctgcgcatt tcttacctca agaacgcggc
    16321 ccgcgccctg cctgtggcac aatggtcaga aaatgatatt tgcttcctgg ctgctgatgt
    16381 gaagaaggat gcagcctggg ttcaacagca ggctgcccaa ctggaagcct gatcaattcc
    16441 cccgattttt gatgacagga ggatgttgta tctatgaacg acgaacgaac ggccaaattt
    16501 gagaagcgtc gcgctgagct tcataagctg agcgacgcgg aattgaaggc gcgcttctgg
    16561 gagctgtgcg accaggtggt tgaacccatt gtggacatgg cccggacgca cacctcgccc
    16621 tcgattgagc gctcggtgct gctgcgcatg ggcattgaca gcgtgacctc gcatggcgtg
    16681 gtggaccggg tgtatgaagc cggtctgctg ggcaagggtg ccgggcatgt ggttctaaaa
    16741 gtggcgcaaa agctgggtaa ggatgtgcgc gccgcggcag ccgcgattat tgaggataaa
    16801 gaaatcctca agggtctgtt ctaaaagagg gatgaagcta tgacgactca acaactcgat
    16861 ccgaacaaga aacttaatat tgaacagatc ctgcaagatc tggagcatta tcacccccgg
    16921 cgcaagggtt ggacctggcg gcgggtgccg gctggcggcg tggatatggg ccccttccat
    16981 tatcgggata tgtctgagcc actgaagcgc agcattggcc tgccggcctc gaaatacttt
    17041 gatcatattg acccgcagcc aagctgtgtg gtgacgactg aaattgcctc cgggcatttt
    17101 gaggatgaca tccgacggat gcgcatggca gcctggcacg gtgcggatca tattatggtt
    17161 atccgcacca tggggcaaag ccatattgac gggctgctgg aaggcactcc ggagggtatt
    17221 ggcggcgtac cgatcacgcg caagcagatc cgggcgaccc gcaaagccct ggatatgatt
    17281 gaagacgaag tggggcgccc gatcaacttc cacagctacg tttcgggcgt ggccgggccg
    17341 gaagtggccg tgctgtttgc cgaagaaggc atcaacggcg cgcaccagga cccgcagtac
    17401 aacgtgctgt accgcaacgt gaatatgatg cgctcgtttg tggatgctgc tgaggccaag
    17461 aaagtgatgg cggatgcctg catcttccag attgacgggg cgcataatgc caatgccacg
    17521 gccaaggcgg gctggctggt aatgcccgaa ctgatggttc agcacggcat caactgcgcc
    17581 ttctcggtgg ctgcgggcat ggtgccggaa ctggtgggcc tctcgacggt accgccgaac
    17641 gccccccccg cccccaaact gtggtacgac ctgccctatg cggtagcgct gcgcgatttc
    17701 ttctcagcct tcaagatgcg cgcccagcaa aacacgcgct acattgaggc ggatattgaa
    17761 gaggccatcc gcacgcacac ggtggatacc ctgatctcga tgctgaccag cgctgatatt
    17821 cagagcacca tcacgccgga tgaaggccgc aacgtgccgt ggcactacaa caacattcgc
    17881 ggcgtgcaga cagtgaagca gacctgggct gcactggatg gcatcaaaga attggtctca
    17941 ctgaaccatg aagggccgct gggccagatg gtgcgagaca tcaaagaacg ggcagtggcc
    18001 ttcctagaag aaatcatcga gatcggcggc tactttgcgg cagttgagaa aggtttcttc
    18061 gttgactcgg ccaaataccc ggaacgcaac ggcgatggga ttgcccgcga cagcaagggt
    18121 ggcattggcg ccaataccat tgtggcgcgc gaccccgact actacgcccc ggtttgcagc
    18181 cactttggcg ataatcacgt gccggcgggt gttgagcatg cctgcgatcc gattggcgga
    18241 tgtacactgt gcgacccgga aaaaattgtc tttgtggacg aactggaccc cgaagataac
    18301 tgcaacaaac ggctggaacg cactcagcct taccgcacgg gcacagccat caaacccgag
    18361 gcggaatggg cgggtgacgg caccgtgctg ctgcaattct ttgtaccact gcctgaagat
    18421 gtggcggcag tctatggcgt ggaaatggcc aagaagatgg ggctggtgga cccagaagtg
    18481 gtcaacatca ccccgatgca cccggtggaa ggctgcttcc tggaggtgaa gggcaaggtg
    18541 gactttgaca ttgaccgcac cacgctgcgg gtgcccgaaa aagagatcat cctgccggaa
    18601 gatgagctgg ttgcagcggt gcggcaggtg gggctgcgcg tggtggctgg aaccgtgggt
    18661 gaagacgagc acagcgttgg cctgcgcgaa atcctggata tcaaacacgg cgggattgaa
    18721 aaatacggtg tgaagtatca ctacctgggc acgtctgtgc cgctgggcaa gctggtggat
    18781 gctgcgattg aaaccggcgc ccaggcgatt ttgatctcaa caattatcag ccacaatgat
    18841 gtgcaccgca cacagatgcg caagctgcat gaactgtgtg tggagaaggg catccgcgat
    18901 aacatcatcc tgattgccgg cggtacgcag gtgagccggg agatggccgc ggagaccggg
    18961 ttggatgcga cttttgggcg cggcaccaag ggcattcatg tggtgaatgc gatggtgaag
    19021 atcctgcggg cgcgcgggtt ggttaagtaa caatcataga aggcccgggg atgttgagtg
    19081 tccccgggcc tgattttgga aagttgatca atctgcactg cgggaaaaat atccgtaagg
    19141 tatcaaggta caacgtgacg caacaaattc tggatgtgct gaccattgag gtcggctcga
    19201 ccattaccaa agtcaacggc tttcggcggc tgccagaagg tggttttgag catggagccc
    19261 aggggtttgc gccgacgagc gtggcggcag gggatgtggg cattggggtg gagcaggcaa
    19321 tcattgacct ggaagcccgc catggcaagc tgggcagccg cccggaaacg tttgtgaaca
    19381 gctctgccgc aggcgggctg cggatgacgg tgcatgggct aacgtactcg atgacggcac
    19441 gggccgcgcg tgaggcggcc ctgggcgccg gtggaatcgt caaaatggtg acggcgggga
    19501 cgttgtctga ttttgatctg gatgagctgc gggagatcaa ccccaatatc atcctgctgg
    19561 ctggcggagt ggattttggc gaaaaagata ttgtgctgca gaatgctgag aaactggctg
    19621 gactgcagct gggcgtgcca gtggtatatg cgggcaatgc ggccatccgc aagcaggtag
    19681 ggaagatttt tgaaggccat ggcattaagg cgttgattgc gccaaatgta tttccagatg
    19741 tggatgtgct gaatattgcg ccgctgcgga aaattattca cgaggtgttc aaccagcata
    19801 tcattcacgc gccgggcatg gcacggtttg ccgagctgac ccactggggc attctgccaa
    19861 cgccgggggc tgtgctgcga ggggcagaac tatttgccga agcgatgggc gactgcctga
    19921 tttttgacgt gggcggcgcg accacggatg tgcacagtgt aacggatggc agcctggaat
    19981 ggacggccaa actgactgag ccagagcctt atgccaagcg caccgtggaa ggcgacctgg
    20041 gggtgttcat caatgcccat aatattatcg agatggaagg cgatccggcc tgggaagccc
    20101 ggctggaaga tctgcgcgcc atcccggcaa cggaacgcga aaaagagctg acccactggc
    20161 tgtgcgagcg cgcggtgcat accggggcgc ggcggcacgc tggcgtggtg acggacctgt
    20221 acacaccgat gggcaagaag cagattgtca aaggcaaaga cctttcggcg gtgcagtggg
    20281 tggtgggcac cggcggagcg ctgacccgga ttgagggcgg cgaggggatt ctgcgcagca
    20341 tctgcacggg gccgggcaaa tacctgctgc cgccgccgga cgcacgaatt ttgatggacc
    20401 gcgattaccg gttttcggcg ctgggcacgc tggcccaggc gtatccggaa gatgtgcggt
    20461 tgacatttga gcgctgggca aacagcaata cctgatgcga tggattgatc ggtaaaccag
    20521 gaaagaggta agcatgtata ttcgaacccc cagacttgag atttatcccc accggattga
    20581 ggagaacgcc cgggcagtgg ttggactgtg ccacaaagcc ggaattcaga cggcctgcgt
    20641 gacgaaggtg accagcgccc acccggcagt ggtgactgcc ctggttgccg gtggcgccga
    20701 tatgattgcc gactcgcgga ttctcaattt gcagtccatg gtcaataccg ggttgagcgt
    20761 gccgctgatg ctgctgcgca ttccggcgcc cagccgggca gccgatgtgg tgcggtgcgc
    20821 cgattattcg ctcaactcat cggttgagac gattgaactg ttgtctgaag cggcgcagtt
    20881 cttaaaagtg aagcatcatg tggtcatcat ggttgacctg ggcgatttgc gggaaggcgt
    20941 gtggcctgac cgcgcggccg aagttgtttg tgaagcggcc aagctgccaa atattgaagt
    21001 ggcggggttg ggctgcaacc tggcctgcta cgggggagtg atcccaagtg tggagaatat
    21061 gcaacggttg atcgaaattc gcgagatctg ccgaaaggcc agtggactgg atttgaacct
    21121 gctctcgggt ggcaattcag ccaacctgcc cctgctgctg gatggcacgt tgcccaaaga
    21181 aattaatcac ctgcggattg gcgaagccat tgtgctgggg cggaatgtga ttgaccgcag
    21241 cccgctgccc ggcacccggc aggacacttt caaaatttac gccgaggtgg tggaagtaga
    21301 acgcaagccc tcggtgccga ttggcaaccg gggacaggat gcctttggcg gctcgccgga
    21361 atatgtggac cgcggcatcc ggctgcgggc catctgcaat attggccggc aagatgtggt
    21421 ggttgacggc atcacgccgg aagaccccgg cattattgtc ctgggtggca gcagcgacca
    21481 cttgattctg gacgtggaag aagccgaaac cctggtggaa ctgggtgccg aagtggcctt
    21541 ttatccaaac tatggcgcgc tgctggctgc cagcacgtca ccctatatgc agaaagttgt
    21601 cgtcagagaa taaattatgc tgtatcaaac ccatgcccgc attcatctca accatattcg
    21661 ctttaacatt gaaggtatcc gcaaggcggt gggagcagaa cgcaagatct taattgccgt
    21721 caaagccaat gcgtacgggc acggagcggt ggcggtctca cgcatggcgg aacagattgg
    21781 ggtggactgg ctgggcgtgg ctaccgtgcc cgaagggatg caattgcggg aggccgggat
    21841 tgggctgccc attctcaagt tgagtccggc gtttccagaa gagatggaag cagccgtgcg
    21901 cagccgggtg acactggcag tgggtgagca gtctaatatt gcggcattgg aagaaatctg
    21961 cgccaaccac ggtctgacta cgcatgtgca tttgaaagtg gacagcggca tggggcggat
    22021 tggcgtgacc gtggctgaag cgccagcact ggcagcattt attgaacggg aatgcccaca
    22081 cctgcatctg gaaggggtct ttacccacct gccggtcagc gattcggctg acccgacctg
    22141 gacacgggcg cagattgagc gctttaatgg cgtggtgagg gcaatagaga caaccgtggg
    22201 gcacaagatt gaactggcgc actgttccaa ttccggtgcg gtgctggggc atgccccggg
    22261 atggatggac atggtgcgcc cggggattat gatttacgga ttctacccgg atgaaggtac
    22321 cccgcgcgcc attccgttga agccaggact gagcttcctg acccggattt cctttcttaa
    22381 aaaggtgacg gctggcacca gcattggcta cgggcgaacg tggattgcac cagaggatac
    22441 ctggatagcc accatcccgg ccgggtatgc cgatggattc aaccggctgt tttccaaccg
    22501 agggcgggtg ctgatcaatg ggcgttctta tcccattgtg gggcggatct gcatggatca
    22561 gagcatggtt aacctggggc cggagaccac tgcgaaggtt ggcgatgagg ttgtgctgat
    22621 tggaaaaagc ggcctcgagg aaattacagt agatgagtgg gcgcgcgaat tgaagacaat
    22681 cacctatgaa gtgacctgcc agatcaattc acgggtggaa cggatttacg accgttatta
    22741 attgacagcg ttttggcaag ccgattatgc actataatcg tgtcaaaatg gcgtatattg
    22801 catgatgaaa tgtttttggg agcaaccatg aacactgccc tgtggatcat ccaaatcatt
    22861 ttgagcatta aactgatgac gacagcctac agccacgggt tgaggcagga tcaagccaca
    22921 atgcgagagg cccggctgaa gcttgggagg ccggggcggg cgctgttgta tgcaacggcc
    22981 atcggaacca cagccggggc gctggggctg attctgcccg gtgtgattgg cactgccggc
    23041 tggatgacgc caacggcggc ggcttggatg agcgcgatgc tgctggggtc catcctgtgc
    23101 cacgtgcgca gccgggaaaa gcccaagatc tttgtcagca ttgtgctgtt tgtatttgca
    23161 gcgctggtgg cttatggccg ctgggtggtg acgccgctgt aaaaaaagca gagggaccct
    23221 atgacagagc aggatgaaat tgaaaaactg aaagagtggc aggagcatca gtttaactcg
    23281 ggttactggg tgggccggct gccgcaggat tttcccctta aacggtcgtg gaagacgtat
    23341 atcatcgtcc tgatccagtt tatttttatt ttggttggaa ccggagtttt gattctggcc
    23401 tacattctga cacgggatct gaatacactg attgcagcgc tatttttgat tgggctcact
    23461 gcagtgacct atttgcttgt taaacgcgtc aaaccgctta aagatgggaa gcctggccag
    23521 gagggaattg aagccggacg ccggtcggaa aaccaggaag agaaatagaa acaacccaag
    23581 cggggaaaag actataattg atttacgcac tcgtttatag agaaggaata aaaccatgac
    23641 aaccgattac gccttgcagg ttgaggttta cccggcaaca gcagaacgct ggccggattt
    23701 agaacagatt ttaagccagc gcagggcgac ctcaagctgc tggtgcatgt tctggcggct
    23761 gcggcggaca gatttcagca agctgacaga tgatgaaaag aaggccgcct tgaaggccca
    23821 cacagcggac cgtctggcgc cgggtgtgct ggcttatgtg gatggaaagg tgatcgggtg
    23881 gtgttcggtt gggccgcggg agaattttgt ggcgctggag aactcccgga ttttgaagcg
    23941 cgtggatgac acaccggtct ggtcgattgt gtgcttcttt gtggatacgc ctttccgtaa
    24001 aaagggtgtg atggctgagc tgctgcgcgg ggcagtgcgc tatgctgaac aaaatggtgc
    24061 gcgggtgatt gaaagctacc cgcttgagat gcaatcggat aagctggcag ggcaaaagct
    24121 gcatggcttc agcggctaca tggggattgc atcggtattt cgggccgccg ggtttgtgaa
    24181 agtgagcgac gcctctgaga cccaggtgat tatgcgctat acagtggcat aaactcacat
    24241 ggaaactgac gatattcttc aggtttttgg actcgatgca atcctgacag aagctgacga
    24301 cccggcacta tgggctacgg ctttgcgcca atactggccg cggcccaacg accccaagtg
    24361 catggtggaa gctctacgct ttgagattgg ccgccggttg gcagacggcg aacagccgca
    24421 gggtgaagat aatgagcgcg atgcgaccat gcgggggatg gtggatattc agaaatgggg
    24481 gtttgaacgg atggatgccg aacggctgca ggtcaaaccc cgcccggcag gcgaggtgcc
    24541 gccggtgatt ctgccggggt tcgggatgat agggttaatg tcggggcagg tgccacctac
    24601 gccgccggcc caggtggttt ttatctatct ggggatctca cggcccagcc gcgcggcgga
    24661 tgaggcggtt gtgttttcgg gcttctcggc gccaggcatt ggcgagggga atgcttacct
    24721 ctggcggtgc gacgaaaccg ggcagtgggt gatgaccgat caacgtgtgg cctggtggat
    24781 tacctgaccc ggcggcatca tgctataatt tgcaggaagc gatcattgct tgcaacaaga
    24841 caacacactc aggagttcca caatgaatca atatccgcct ctggccaaaa agttatttgc
    24901 agctcaaatc cgatttgctg gtattatgag cgtcgtggcg ctgatcatgg gattggtcta
    24961 tcgagaaatc tcgcggggat tttttgacgg cctgtcttat gagcagcaac tgctatatgg
    25021 gcacaatatg agcctggtgc acgggcatac cttcctgctg ggagcagtca tcccgatggt
    25081 gctggcagtt ctgacactgc tgacgctgcc caacctgcca gaaaagcggt tgaagaatat
    25141 gaacgtacgc tttacggcct atatgattgc ggcaagcgtg gcactggcgc tgatggttta
    25201 taagggattg gctttcatta ttagcgcagg gcagccgctg gaagcaattg atgcgggact
    25261 gttctttggc agcggactgc tgcgcggtat tttgtttggc ctgtcgcatg ttgttatttt
    25321 ctgggcagtg ggagagtatg cagttggggc tatccgggca gccggagcca gcccacagaa
    25381 ggcttgaacc aactgattga cagcacatca aaagcgctcg ccatccggtg ggcgcttttt
    25441 tgatgctgat tatgtaaatg agttgcagtg gtacaatggg acgcattgga gaaaaataga
    25501 tggcacttca gaaaacaggc aagaaaacat cgaaaattgg attgattttg ctggcctttg
    25561 tggcgtttat tgcattggga atgccggatg ggctgctggg cgttggctgg ccctccattc
    25621 gggcgggttt tgggatccca ctggactcgc tggggatgct gttgattgct tcaatgaccg
    25681 ggtatctggc atccagcttt ttgagcggca agctgattgc ccggatgggc gtgggcgggc
    25741 tgctggcggc aagctgcgcg gcaactggca ttggattgat tggttatacc ctggtgcctg
    25801 cctggggaat gatggttgcg ctgggggtga ttgccgggct gggcgccgga gcgattgacg
    25861 ccgggttgaa tacctatgtg gcggcaaatt tcggtgaagg gttgatgcag tggctgcacg
    25921 ccagctacgg cattggcgta accctggggc cgattatcat gacactggcg ctgaacgcgc
    25981 tcaactcctg gcgggtgggg tatgcgattg tcggcgggtt tcaactggcg ctggcagcct
    26041 gctttgtgct gaccctgccc atgtggaccc agaaagcaca cccggcagag gcagagaaac
    26101 ctcagcggct gactgactac aaaacatcgc tgcgggagac actgcgccag ccgcaggtct
    26161 ggctcagcat cagcctgttt ttcctgtaca cgggcggcga ggtatcgctg gggacctggg
    26221 cctacacact gctgacggaa tcacggggaa ttgaccccca agcagccggg ttatgggccg
    26281 gcagctactg gctgatgttt accatcgggc gggtgctggc agggctgtat gccaaacgga
    26341 ttaagggcaa gctgctggtg cagatcagcc tgctgggggc actgttgggc gccgggctgg
    26401 tctggtggaa tctatcggaa gccgtcagcc tgattggcgt ggcgctgatt gggtttgcaa
    26461 ttgcgccaat cttcccggcg atggtttcag gtaccagcca gcgggtaggg gaccggtttg
    26521 cggccaatac cattggaatg cagatgggtg cagccagtct gggggcatcg ctgatcccgg
    26581 ggttggtggg ggtgctggca aaaaacatct cactggaagt gattccggtg tgcctgtttg
    26641 tgctgtttgg cgcgctgctg ggcgcgtacc tggtggccac ccggaaacgt gaggcgaaag
    26701 ctgtgcggga agaacaccct gctgcggcag agatgtaaaa aacagctttc agggagaagt
    26761 aaatttgatt caaacgcagc gattaatcat ccggcatttt cgggcagagg attttagcgg
    26821 cctgtttgat tatctatcca atccggaagt ctatcggttt gaaccgggcg atagcatcac
    26881 actggaggag gctaaaacgc tggctgccca aagagccgag tcatcagaat tctgggccgt
    26941 agccctcaaa gagaccgata aaattattgg gcatctctat tttcatcaga tcgaaccgca
    27001 ggcgctgaaa acctgggaac tggggtatat ctttaacccc gcctaccaac accagggata
    27061 tgccgtcgaa gcggcaggcg cattggtgcg gtatggcttt gccaagtggg agattcaccg
    27121 cgtggtggca tactgcaatc cgaagaatac ggcctcctgg aagctgatgg aacggattgg
    27181 aatgcggcgg gaaggctatc tgcgcaaaaa tatatttttt caaaccgatg ttgtggatga
    27241 gcccatctgg ttggatacat tggcctatgc aattttggag gaagatttag ctccggcaga
    27301 agtttagcgc ccgcggaaat ttgtcgtata attttctggg agatagaaca tgattaccat
    27361 tgctgagttt tcaaaaatgc tggcgaatga aaatgagatt gtgcacgaac agaccaggga
    27421 attgagcaat gccgacgcgc tgattcaacc acagccaagc ggcaactgtc tgaactgggt
    27481 gctggggcac atgctggata atcagatcct ggtgctgaag acgctggggg gcagttctcc
    27541 aattgagcga actgagacgg agcgctaccg gcgggattct gaatccattc gggcagaggg
    27601 accgggcgtc tggacgctgg aacaactgct ggcaggacat gatcaattgt tagcagcgct
    27661 gacggcacgc ctgggagaac tgaccgaaac cgatctggac gaggaaattc aactgggcga
    27721 gcggaaggtc aagcgcggct ggtgggtttt ctttctgcat tttcactata cctatcacct
    27781 ggggcagttg gagtatttgc gccagttggc cgggaaaacg gataaagtca tttagggcgc
    27841 gagatggaat ggtctccaca aaagaaaatt gcttatggca ttgcagctgc ctgtattctg
    27901 attccctgcg tggttttatt ctttaacctg aaggctgggg cttttgctct gtttgtcggc
    27961 gctatctcgg cgagccaggt gatccgatac gcaaatcaag ccgacccaca gatgagcgcc
    28021 gcaacccgtg cccggctggc gcggcaccag agcgaccggt cgcgaaccat ctatgtgcag
    28081 gtggtggacg acaacggtta tgacctgccg cccgaggtgg tggagcagaa gatgaaagaa
    28141 gcgcaactgc aagccgggcc gcgcgatacg gtgattgctg tacggcatca ggtgaatcaa
    28201 tgactctgac aatgggtgca gtgacgctgg ttgtaaggga ttacgatgag gcgattgcct
    28261 actttaccaa cgcgctgcga ttcacgctgc tagaagatac gcccctgggc aatggcaaac
    28321 gctgggtcag ggcagcaccg ggaggatcaa atggctttgc gctgctgctg gcacgggcag
    28381 ttaccccgga acaggaaagc cgcataggca accaaaccgg ggggcgggtt tttctctttc
    28441 tctacactga ccattttgag gatgattatc gccatatgcg cgccagtggg gttgaattta
    28501 cggaggagcc ccgtcaggaa gcttttgggc gggtggtggt ttttagagac ctgtatggca
    28561 ataagtggga tttgattgag agtttgaccg gggggtgaaa acctcgaaag ggcgtgctat
    28621 actgattatg ctttttctga caggcacacc cgattgaaag aggtttccca atgaaaacag
    28681 agaaaaatat tgtcctctgg ggacagggtc tggtcatcct taatgcggtc atctggatgg
    28741 cggttgggat tgtatggctg cttcggcagc actcaccggc cgtcatgcct ctgtgggtgg
    28801 tatggggaat ggggattgcc atgatcggct acggcggggc gctgctgttc ctgggggttc
    28861 agttgcggcg gcagaagcag ggcatctacc tggcagcggt ggcactcatt tctattgcga
    28921 tcattgccgg gttgtttgat gacctggggc tggcagacct gctggggctg ctgccggggc
    28981 tggcggcgct gatctacctg tggatttata aaaatgtact ctcgggggca ggccctgctc
    29041 caaaaggaaa cagccatgac gcgattagag tccatcaagg gtaatgtgct ggagtggctg
    29101 ctggaagcgg attcgccggg ggtgcgctat ctggtgctgc gcgatttagc cgggcgttca
    29161 gaaaatgacc cggaactgaa agccgcctgc cgggcggcac atcagcaggg gccgattgct
    29221 aaggtgctgg atgccatgtc acctgaagga tactgggtac agcccggcgc gggttatgcg
    29281 cccaaatacc gcggctcggt gtgggcgatg atcttgctgg cacagttggg cgccaggatt
    29341 gaccaggacg agcgaattgc gactgcctgc cagtatatga tggagcatgc cctggcccga
    29401 caggggcaat tctcgtataa cggggcgcca tctggcacct ttgactgcct gcagggcaac
    29461 ctgtgctggg cactgcagga attgggctgc ctggactccc gattggatgg ggcgtttgag
    29521 tggatggcgc gcagtcagac tggcaacggg attgccccga tgaccgataa aaaggccgag
    29581 gtacggttct acacctataa gtgtgggcca aattttgagt gcggcgccaa taaccgccag
    29641 ccctgcggct ggggcgccgt gaaggtgatg ctggcattca gcaaactgcc gccggagaaa
    29701 cgcacggctg aaattaagca ggccattcag caaggggtgg attttctctt cagcggggat
    29761 cctgccatgg caaattaccc cacgccagaa ggtcaggcgc ccagccataa ttggtggaag
    29821 tttggctttc cggttttcta tgtgactgat ttgctgcaga atgttgaggc actggcgggc
    29881 ctgggatatg gcaacgatcc ccgcctgagc cgggcccttg agatcatccg ggacaagcaa
    29941 gatgctgatg ggcgctgggc catggaatat gattacacca gcaagacctg ggtcaatttt
    30001 gggccgaaga agcggcccaa taaatgggtc acattgcgag cgctgcgggt tttgaagatt
    30061 gctgaacaga aattataagc ggcatggggt tgtaaaccag attggcggct ttacttacac
    30121 ggtcagagag tgtctattca aatgacgcta aaatctctgt cacaacttct tccaggttgt
    30181 attcgcggcc ggcttgcaag ccagctgcat gtacagcctg gggttgtgtg tgctgcaaat
    30241 ctgccagagc ctgttcagca attgtccggc tctcgtcttc caatccgggg tgattcaaga
    30301 cgaccgataa caccatcagc gcctgctctg aacggtttgt gtgcgtcagg acgcctgaca
    30361 ggccggataa aatatccaga atatcaggga tgtcattaat ggcggctgcg gtttgtaatc
    30421 ctttgcggta gtaggcttca gccattggat aatcatggca ggccagggca gtgtgcccca
    30481 ggttgcccag ggtgatggcg gcaaggagtt catcacccat ttcatggtca attttcaagg
    30541 ccatttcata atattgacgc gccgcggcgt aatcaccttg acgccgggca gcctcgccca
    30601 gattgaccag ggcagcagat tcgccaggcc tgtcactgac cgcatgaaat aaggccaggc
    30661 tttcctgata aagctgacgg gtctgctcat actcgcccag gttgagcgcc acagtgccca
    30721 gcccgttcag ggaatcagcc agcccatgcc gatcatccag agctgcataa atcgctcggc
    30781 tctcaaggag aaacgctctg gcttcagcat aagctccctg gcgaatattc aaccaaccca
    30841 acgtgtgcag ggcatgggca atccggcttt gatcaccgag gcggcgggca atttccagac
    30901 tctcattcag gaaggtttgg gcctgaggcc agttgccctg tttcctggca attcctgcca
    30961 gatgaatcag ggcatcagca caccaccctt cttcaccgtg tgcttttccc aaagacaatc
    31021 cttcgtgcaa taaattctga gcctgctcat aggaacccag ataccataag gcttcaccaa
    31081 actgcaccag taaggggata cgatcccgat ttccggacgg cagtaaattt aaggcgcgag
    31141 aaaaataatc ggcggactcg cggtaagcat aggttcgcag cgcgccaaca gccgcacgcg
    31201 ccaggtattt gaccgctttt tcagcattac cggcgcgctc gaagtgttca gcaatctggc
    31261 cgataaattc cgattctcgt tctgctgaat gagaaatcaa ccagtcggcg gcgcgggcgt
    31321 gataagccgg gcggtcgcgc ttgaggatgc tctcatagac agtgctgcgc agtaaagtat
    31381 gttggaaaat atattccgcc atggcagcga aggtaggggt atctgattga aaaatcattt
    31441 cccggctgca caacgcagac agagatcgtt cggtatctaa acgtgcggcg cctttggcaa
    31501 taaaatcaac tgctttttcc caaaatgtgc ggccaaacac agaagcacac tgcagggtgt
    31561 ttttttctag agccgtcagc aagtcaaagc gggcttgaag cacagcggtc aaggtggagg
    31621 ggacacgcac cacggccaac cgatcggggt tcacctgcca gtgttctatt ccctttgtga
    31681 caacaccatc ttcaatgagc attttgagca gttcttccat gtaaaatggg ttgccttctg
    31741 cgttattaat catcaggctg cgcagcatcg gggggatgga ttctaccttc tgtaaaatat
    31801 cgtccaccaa ccgctgacta tcttgctcgg gaagggggga caggttgagg cgattctgaa
    31861 caggctgccc gctggcccag gaagggcggc gatcaaacaa gctggggcgg gctgcagtga
    31921 tgatcagaac cgggtgccgg cttaacatgt gccctaaatg ctcaaagaca tccaaagagc
    31981 tgtcatcagc ccagtgaata tcttccagca agatcacaat ggtggaagat tctgtcagcg
    32041 atttaaagta atcgacaaga tatatgagtg cgcggtcacg gatctgctgg gcatcttcca
    32101 ggatttctgt gagatatgga ctttcgctca ggtcaaagcc aatccaatgg ccgatgatgt
    32161 gagcccgcat aggagattcg ctggcgggcc aggccgcacc ggatacagat gccccggcgc
    32221 caataccggt ctccattttt tcctgcacag ttttcagtgg atcgcgatct tggatctgga
    32281 agcgaaaaga gaaaatatct cgcagcaagg catagggaac agattgcatc tcttgactgg
    32341 cgcgaccgcg aatgacgaca cacgattttt gatacgtttc gagccaggcg tcaaactctc
    32401 gcaggagccg gcttttaccc aacccggctt cggcgacgag cgtgaccatt tgcaattttc
    32461 gctgttcggc aacagttccc aaagtggatt gcaggtaagc caattccaca tcccgcccaa
    32521 tcatgtgggt tcggattcct tctacaccac gggttgccac ccagaattgg cgttgttttg
    32581 ctcgcagcac aagataggtc tggacaggtt cggctttacc tttgaccgtc aacggcggtt
    32641 gaacctgaac atcaaacatc ccgcttacat gacggtaggt attgtgcgcg atcaaaatgc
    32701 catctactgg agcggcactc tccagacggg aagccaggtt aactgtatcg cccatggctg
    32761 taaactcatg ggttgagcct acctcaccta acagtaccgg gccggtgttc aggccgatgc
    32821 gaatggataa ccccagatgg cgctctttgc aaaaggcagt caaagctgcc tgcattgcca
    32881 gagctgcctt aatggcacgt tcaggatcat cttctcggac aaccgaagtg ccccacagtg
    32941 ccatcacggt atcgccgata tgtttatcaa ttgctccacc gtgactgata atgattgggt
    33001 ccagccgctt ccaaacatcc tggatcatgt cccgcgactc ttcaatatcc aaattttctg
    33061 aaggcgctgc cagaccagtg atgtttgcga aaagaactgt aatctgcttg cgttgatttt
    33121 cggctaactg tgcctggccc tgaagcaggg ctaatttctg gcgcattggg gccagggcag
    33181 cattaaccac gtcatctccc aaagatgatc gctgtgcctc aagagcagca atggcatgga
    33241 gcaaggtttg aatttcggtc attaaattat gatcgggcta taattgttag aggaaagttt
    33301 tgggactggc tgcatcatcc agccagagcg aattatatcg caccaggtaa gaatccgaat
    33361 atccagaatt attatttcca gtcaggagaa tcattcatgg ttaaagttcg cgaagccgag
    33421 ttgaaagact gccacgggat tgcccgggtg caggttgaca gctatcgcac ggcgtatgct
    33481 ggtttgatgc ccgaacccta tctggcgcag ttttcttatg aggagcagga acaggactgg
    33541 gttggactgc tggcggcaga cagtgcggat attctgctgg tggctgaatc tgaggaaggt
    33601 gtgattgtag ggtacaccct ggctcgcgct gaggcaggga tttatccggg ttacgatgcg
    33661 gaaattattg cgctgcatgt acgcaagtct gttcagcggc agggggtggg acgcgccctg
    33721 ctgcgcgggg cggttgagaa actgatcgcg cggggctgcc aatcggtgat gctctggaca
    33781 ttgaagggca acccggtgcg gcgttggtat gaaaagctgg ggggaaaata cctgggtgaa
    33841 aagacctggc ccctggatga ttttgaggta cacgaagtag cctatggttg ggacattgga
    33901 tgacccggtg gggtttctgt ccgggttagg ctggaaagtg tcattaagtc aggccgggca
    33961 gcctgatgcc aattttggac gctggacact gccggtgctg ccaaccgaaa tgcctaatgt
    34021 tccgcataac tggtttgtga cggcgcagaa gttgt
//
LOCUS       DAOPVS010000014        33395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1244_length_33395_cov_59.239472, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000014 DAOPVS010000000
VERSION     DAOPVS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 33395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33395
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_33395_cov_59.239472"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /gene="malQ"
                     /locus_tag="PKL11_02740"
     CDS             complement(<1..1439)
                     /gene="malQ"
                     /locus_tag="PKL11_02740"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNZ12546.1"
                     /translation="MEFTRASGVLLHPTSLPGPDGIGDLGPECYRWIDFLAGCGCGLW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISPALLWEDGLLTRADLADRPAFPAERVEYG
                     DVIPWKLKVLERAEKRFETAAPAALKEAFEEFQKAQAFWLDDFALFMAIKENQHGVGW
                     ENWEPEFRMRQPEALAAFQSANAGAIRRQKLRQFLFFRQWRAVHDYAHKQGIQIIGDI
                     PIFVAFDSADAWANPELFYMDKNRRLTAVAGVPPDYFSPTGQLWGNPLYKWEAHRKTG
                     YAWWIQRFRAILEMVDIIRLDHFRGFAGYWEVPAGSTTAEHGQWVPGPGKHFMAAIEK
                     ALGSLPIIAEDLGEITPDVIELRDSFNLPGMKILQFGFDGDATHAFLPHNYPHNCVAY
                     TGTHDNDTVAGWYASAPEVERDLCRRYLARSGDDIAWDMIRAIWSSVAVQALAPIQDL
                     LSLGTEARMNFPGKTGGFWSWRLPTEALTP"
     gene            complement(1491..2999)
                     /locus_tag="PKL11_02745"
     CDS             complement(1491..2999)
                     /locus_tag="PKL11_02745"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNZ12547.1"
                     /translation="MKANPTPLRVLFIASEADPLVKVGGLGDVAGSLPRALQALLPAD
                     LDGRQPDIRLALPFHSAITQKLPEIQLVASFTVPHATGEIAGRAFETKVQGVTVYLIE
                     GAPIPPNAPVYNTQNSREDGEKYVFFSLAALEVVKKLNWKPDILHANDWHTATAVYAL
                     ALRRASDPFFAETRSIISIHNLPFMGTGAEPVLTAYGIPPAQDERLPHWARLFPLPLA
                     LLTADHIVAVSPNYARELLTPAFGCGLQDFLSTRQEALSGIINGLEVSAWDPATDATL
                     TANFNADNLEARAKNKQALLEEMELSALPDAPLLIMIGRMDNQKGVDLAIEGLRLVTD
                     QPWQAILLGTGDPLVESAARSLEVELPDRVRAVIRFDGKLARRLYASGDMLLMPSRYE
                     PCGLAQMIAMRYGCVPVARQTGGLQDTIRDNREADGTGFLFGDASPTAFTVAVLRALA
                     LYPNRNAWREMQLRGMREDFSWQPSARAYGRLYLDLLQRPPLPRPEVGFSES"
     gene            complement(2996..4966)
                     /locus_tag="PKL11_02750"
     CDS             complement(2996..4966)
                     /locus_tag="PKL11_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ12548.1"
                     /translation="METVDLILTNAIVVTMDELMHIYDPGAVAVRGDQIVAVGPMGEM
                     REHFTASEELDCGGKVLMPGLVNAHTHIPMTLLRGLADDLRLDVWLLGYMMPVEREFV
                     SPDFVRLGSQLACAEMIRSGTTCFADMYYYEEDIAKATAEAGMRAVCSQTVLKFPTPD
                     AQFYEDSLAATRDFIVRWKNHPLIVPSVAPHAPYTCTPEILQACAALAAEFDVPLHTH
                     LSETASEVETMRNEQGMPVIPYVKKQNLFDAKVLAAHCVHIDEGEMRTLKHAGAGVAH
                     NPSSNLKLASGVAPIRRMIDLGLNVGIGTDGPASNNDLDMFEEMRLAAFIAKGTSGDP
                     TALPASTVLLMGTRLGAQALHLGSLTGSLEPGKRADLILVDISPAHNMPRFRRDPAGI
                     YAQIVYAAKSTDVSDVMVNGKWLMRERQLLTLDENELLHHSQEYATRIDAFLFQREKS
                     ILSKLVAIGGATEEESYEVQIKVRIPDAQPVLAALQNPAISVVRKRHYRQYDTYFCFT
                     DPDQGYLRYREDHFISDKGEITNVRSRLTHIGPAREAQYSQKVLLSRSRFLAPAAQTA
                     RFYREYFKPAMEMEIEKDRLRFLVQYEGIEFFINIDQVQRPALGYYLEIKSRTWSRQD
                     AKDKSRLAVELIELLGATTEEATSEDYLDMIR"
     gene            complement(4974..6131)
                     /locus_tag="PKL11_02755"
     CDS             complement(4974..6131)
                     /locus_tag="PKL11_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNZ12549.1"
                     /translation="MPSCEIIAIGTELLLGEIQDTNTQYLARNLRDVGIDLYRTTMLG
                     DNAERIAQTIQECMGRAEIIITTGGLGPTVDDPTRQAVAMAVGVPLEYREDLWLQIQD
                     RFKRFGRLATENNRRQAYIPQGAQAVENLVGTAPAFIYDTGDRVIISLPGVPREMEYL
                     MENVIFPFLKERYGLRGIIKALVLHASGIGESQVDELIGDLETLRNPTVGLLAKGGQI
                     DIRITAKAESLEQANQLLHEYAILIRRRLGEAIFGQDTDTLESVVMTRAKSSALTLAV
                     IECGLGDELSKRLKAAGLAECCIRTIESPCTEEELREHLRKLQAEQPVDIALAANLTP
                     GQWQQDLLLIAAGPSETHTLQRSWGGPASMSITWSANTALDFLRRIITSEG"
     gene            complement(6169..6681)
                     /locus_tag="PKL11_02760"
     CDS             complement(6169..6681)
                     /locus_tag="PKL11_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ12550.1"
                     /translation="MSKYAVFLKETELFYNLTATQLELMDSICEERVYKKGEKIFEEN
                     SREDELYLILQGEVQITMDPTLVAADPKAAAQPATIAVLRRGQCFGEVALVDQGVRTA
                     SAVALEKGTQVLRIPRGKFLLLCNSYPELGYRVMFNLATELAQKIRGADLQIRAAMLY
                     QVVEDEPKSK"
     gene            6824..7195
                     /locus_tag="PKL11_02765"
     CDS             6824..7195
                     /locus_tag="PKL11_02765"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNZ12551.1"
                     /translation="MPENHKQQLGHWGEQIAAHHLMEHGLTVIARNVRTPYGELDLLA
                     RDGEVLVVVEVKTRASANFGLPEEAITPQKRTHLLQSAQHYLQQHPELDGDWRIDVIA
                     IRRQPGSQSVEIIWFKDAVSQ"
     gene            7179..8111
                     /gene="miaA"
                     /locus_tag="PKL11_02770"
     CDS             7179..8111
                     /gene="miaA"
                     /locus_tag="PKL11_02770"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNZ12552.1"
                     /translation="MLFLNSPAPLLVLVGPTAVGKTELSLELAERLNGEIISADSRLF
                     YRGMDIGTAKPTLAERQRVPHHLIDVTDPADNWTLTIFQAAAAQAIAEVQARGRLPLL
                     VGGTGQYVWAVIQGWAPPEQDPDPALRDILERWGRQIGPEELHRRLRRIDPAAAARIE
                     SRNLRRTVRALEVIFTTGQRFSAQQRRTGSPYTTRIIGLQRPRPELYARIDARIDAML
                     AAGLVQEVQALLEGGCPPNAPALSAIGYREIGQYLQGKITLPEAIVEMKRLTRQFVRR
                     QGNWFKTDDPQIAWFDAASITAAELETQIRSWTV"
     gene            8135..9772
                     /locus_tag="PKL11_02775"
     CDS             8135..9772
                     /locus_tag="PKL11_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNZ12553.1"
                     /translation="MEKPWLQHYDAGVPASLNYPPQTVHGILEATAACWGSQPATCFA
                     DRFLSYSQINALADQLARALLILGLQKGDRVGLLLPNCPQFVLAFYAILKAGGVVAAI
                     NPQYRPRELEAQLQDAGAEILISVRSAAQVIQSVQPHLNLRHIIWTDLEDAPALADRP
                     SSPPAFYPAPEMDFLSILQAPLLPAALPVITPEDVAVLQYTGGTTGTPKGAVGLHRNL
                     AANTLQFRCWLSGLTDGREVALVAIPLFHVYGMVIGMSVSVALGASMVLLPNGRDITA
                     ILQQIEQYHATLFPGVPALYHAVNQHPDVLAGRYNLHSVKACISGSAPLPVTVKAEFE
                     QLTGGKVMEGYGLSEAPTATHCNPMLGENRPGSIGLPLPDVDCRIVSMADGQTPLAVG
                     EAGELLIRGPQVMQGYHNQPSETAAALADGWLHTGDITRMDADGYFYLVGRKKELIKV
                     SGYQVWPAEVESAIITHPAIKEVGVAGVPDEARGEAVKAWVVLHPGQSATADEIRQWC
                     LDQLAHYKAPREVIFVSALPRTPVGKLLRRELINLTA"
     gene            complement(9834..>11712)
                     /gene="ftsH"
                     /locus_tag="PKL11_02780"
     CDS             complement(9834..>11712)
                     /gene="ftsH"
                     /locus_tag="PKL11_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNZ12554.1"
                     /translation="DNYLEVAVNQRNSSVLIYILLFIAIATMIYVGFNRNNTAEELTI
                     NQLALDIQDGKVKVIEEDDNNLIVTYNDGKENKLVKEPGATLVEQLYQFGVTSEDLAP
                     DKVKIVIKNPSVRANVVTALAIVLPLLLMVGVFFFIFRQAQGSNNAAISFSKSRARMF
                     TGDHPTVTFADVAGVDESKEDLQEVVEFLREPQKFLQLGARIPKGVLLVGPPGTGKTL
                     LAKAVSGEAGVPFFAISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIVFVDEIDAVGR
                     QRGAGLGGSHDEREQTLNQLLVEMDGFDTDTNIIIMAATNRPDILDPALLRPGRFDRR
                     VVLDRPDMRGREAILKVHSRGKPIEPAVDLGVIARSTPGFVGADLENLVNEAAILAAR
                     RNKRSIGMSEFEESIERVIAGPERKSRLINPEEKRIVAYHEAGHAIVMNVLPESDPVH
                     KVSIIARGMAGGYTLSLPREDRILMPRKKLLAEMVTLLGGRAAEEIVFDDITSGAAND
                     IERVTQVARSMVTRLGMSSELGPMIYGQKEELIFLGREISEQRDYSEAVAERIDQEVR
                     RLVSEAYDEARRILTQYRQELDAIANKLLEVETLSREEFEAIFPPPVPKKSGIPAMNM
                     N"
     assembly_gap    11713..11812
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11984..13168)
                     /locus_tag="PKL11_02785"
     CDS             complement(11984..13168)
                     /locus_tag="PKL11_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNZ12555.1"
                     /translation="MSLTLLLDLDDTLFDNPMGAFQKAYLKALGAHLNAVVPAERMIP
                     QLLFATEQMTQKQTPVGTLEETFDRHFYPMLGLEKAQLSDMIYGFYTTVFPEIRQYTK
                     PRSAAIELVAEARRRGWTVAIATNPLFPRVATEQRIKWAEMDSSDFALVSTYERFHFA
                     KPQPAYYAEMLAQLGWPEGPVVMVGNDAADDIEPAAALGLHTWLLQPEKLNGGGPLEA
                     ILPWLEDVEAHGHATAWDTPVALRAALHSTPAALDALCEALPEAVWTHRPAAEEWSLV
                     EIIGHLRDVDREINLPRVRLMSSGEESNPFLPGVSADGWVTERHYAGEDGPTALREAV
                     AVRCELLDLLDSLPADAWQRTARHAIFGPTRLLELVGFMATHDQNHIRQCRQAIEAAV
                     RP"
     gene            complement(13560..14123)
                     /gene="efp"
                     /locus_tag="PKL11_02790"
     CDS             complement(13560..14123)
                     /gene="efp"
                     /locus_tag="PKL11_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNZ12556.1"
                     /translation="MIDVNDLRKGVTFELDGNLFKVLEYSHNKPGRGNATIKVKARNL
                     RSGTTLEKTFQSGERVQDVRLDFHNAQYLYNDGDLYYFMDTETYEQYTIHRGIIEDAI
                     GFLREEMEVKLTFYFSEAIDIELPTSVDLKVVEAEVAVRGDTATGVTKRVKVETGASV
                     DVPNFVNQGDVIRVDTRTGSYVTRVTN"
     gene            complement(14263..14820)
                     /gene="raiA"
                     /locus_tag="PKL11_02795"
     CDS             complement(14263..14820)
                     /gene="raiA"
                     /locus_tag="PKL11_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNZ12557.1"
                     /translation="MDLKVDVIVRNMEATDRLNDYAVRKVSKLDRFLGDIDEARVDLS
                     HVKSARNAADRHVAQVTLRGKRFILRTEERAEDIFAAIDAAVDKMQRQIERLKGKRQH
                     GRGDGTPASEVAPVKVEDEIELPVIARRKKFSLSPMDELEALEQMTLVGHENFFIFFN
                     VNTNAINVLYRRRDGSYGLIEPYIG"
     gene            complement(14893..15654)
                     /locus_tag="PKL11_02800"
     CDS             complement(14893..15654)
                     /locus_tag="PKL11_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNZ12558.1"
                     /translation="MTFVGQAKRPPRYVIHQLLWQAADWLYPPVCGGCDAPGERWCAK
                     CQEQTVLLENMAICPHCGVPNHGGEICTDCAAQPPPYTALRSWAVYAGALRKAIHRLK
                     YQGDLGLGEALGAKLVAMWQSLDWQIEMIIPIPLSAQRKRERGYNQSSLLARPLSLAT
                     RLPLCAQALRRTRDTASQVKLTGSERRQNVRGAFTADARQVAGRAVLLIDDVATTGAT
                     MLAAGEALQAAGAHNIYALTLARAGLTNHPDSLTG"
     gene            complement(15668..16615)
                     /locus_tag="PKL11_02805"
     CDS             complement(15668..16615)
                     /locus_tag="PKL11_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ12559.1"
                     /translation="MGTAIIIILGVLLVGGAVTLTIVGLRRPQAEEQDRELLARLEEF
                     SAAGEEVDLRKLELSQPFTQRVIYPMARKLGEIAIRFTPQKMLQSISRKLELAGSPAG
                     LDATMILAAQFIVAAVFAGIVILVFSVGSTVWPITRIILLTIGIAAVGFYFPQLYLTS
                     KVGRRQKEIRKAMPDALDLLTICVEAGLGFDAALAKVSEKWQTELSLAFARCIREIQL
                     GKTRRESLRDMADRIGIPEMTSFVAAVIQSEQLGVSMSKVLRIQADQMRTKRRQLAEE
                     EAHKAPIKMLLPMGGCIFPSLMIVLLTPAGIRLVQGFMQ"
     gene            complement(16637..17614)
                     /locus_tag="PKL11_02810"
     CDS             complement(16637..17614)
                     /locus_tag="PKL11_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ12560.1"
                     /translation="MAGIPPLTILLVVGGIVIVALIIGVVIATRSERDFVDDRMEKYV
                     TTQEEVDERQKALAKTPLTSWLNKRVEKSAFGSKMATDLARADLKIKPGEYIAVIIIA
                     IIGLAGLGYVISGQSIFGALAGGVIGLWYPGQYVKSQQKKRLIKFNDQLADMLNLMVN
                     GLRAGFSTMQAMEAVSRELPPPISDEFMRVVQEMQLGVPMERSLANLLGRIPSDDLDL
                     VITAINVQREVGGNLAEILDTISYTIRERVRIKGEIRVMVSQVMYSGKFLAMMPLFVI
                     GILYLLNRDYIMTFFDEKSGIIGYIALGLAATLIVSGYLIMTKIADIEV"
     gene            complement(17651..19042)
                     /locus_tag="PKL11_02815"
     CDS             complement(17651..19042)
                     /locus_tag="PKL11_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNZ12561.1"
                     /translation="MSILRRITGEGDSGQSGSQTPGSSSMPPARVPMPSMGGQQDKYT
                     DIKNRVQTKLLTTLNPQMDVSRVDEVRRAILELFEQILDEENIVLSRPERNRMFEQIV
                     AEILGFGPLSALLDDDSTTEIMVNGYKNIYVERKGKLFKVPMSFESNEHVMRIIERIV
                     APLGRRIDESSPYVDARLPDGSRVNAVIPPISLVGPVITIRKFSKKPISIEQMVDYGS
                     ITAEAVEFLRAAVIARINIIISGGTGSGKTTLLNVLSGYIPDDERIVTIENAAELQLR
                     QEHVVTLESRPANIEGRGEITIRQLVVNALRMRPDRIVVGEIRDEAALDMLQAMNTGH
                     DGSMTTAHSNSPRDTLSRLETMTMMAGMDLPARAIREQVSSAIDLVIHQERMRDGTRK
                     VTYISEVTGMEGEVITLTDLFFFEQTGLNPNTGKVEGRLRASGLRPKFMDRIEAAGIK
                     LGPNIFGIGDRRR"
     gene            complement(19066..20319)
                     /locus_tag="PKL11_02820"
     CDS             complement(19066..20319)
                     /locus_tag="PKL11_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12562.1"
                     /translation="MPAPEKIKVLIVDDINETRENIRRMLQFDTNIEVVGAARTGREA
                     IEMAQQLLPDVVVMDINMPDMDGITATENIRRKVPFAQVIILSVQNDSSYMRRAMLAG
                     ARDFLSKPPMIDELTSAIRQAAVVAHEERSKAAPPAYTAAPGGMPTLAGMMAGSAQHK
                     GRVIVVYSPKGGTGCTTIATNLAIALHKPDAPAAVIDGSLQFGDVAVALNEQGKNTIL
                     DLAPRVDEMDADVVREVMVVQNTTGVSMLAAPPRPEMAEKVNGEQFGKVLRFLQSMYS
                     WIVVDTSSYLSEPVLEALNNADITILVTTQEFPAIKNCNLFLSLIDLMRIPRDSIIFV
                     MNRYDKRITIPPEKVGESLRQPIMLVLPLEEKVSYYLNRGISFFVDNKTLPISKSIQA
                     LADLVQEHINKPKAGQEGSSDGRRK"
     gene            complement(20363..21535)
                     /locus_tag="PKL11_02825"
     CDS             complement(20363..21535)
                     /locus_tag="PKL11_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12563.1"
                     /translation="MAGGRKKSGLLVVLLTLIVVVVVGIALYYFFVLRPQANNGGLGV
                     GPSGEVEPTAPPPTDLVDIFITLQDVRRGEVLTEANLGTIQYPRAELEASGGIFIVAD
                     SEDIKKEELLGLTAVYDLKARMPLTMEMLVDAGGNAWSPSFDVPVGMTALSMPINDLT
                     AVAYALQPGDHVMVIGCMWILDYDVEFQTPLPNAAGFLAETTSVNADEGRAILPVTAV
                     VNSGTEEDSVLGNEFNIKADGTEYQYQPIGRVEDLQGFKFYVQPSETQRPRLVCQNII
                     QDAIVLKIGSYFDDAAAQAAAAETNAEGGEETAPPPAIAPTLATLMMPPQDALRLNYM
                     MLSGIRISLAMRNPTDSTIYLTDAVTQQYLMDQSVIPLPAKLPYGVETVPMPLPED"
     gene            complement(21732..21878)
                     /locus_tag="PKL11_02830"
     CDS             complement(21732..21878)
                     /locus_tag="PKL11_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNZ12564.1"
                     /translation="MLFAPKEKGQGLVEYALILVLVAVVVIVILALLGPIIGKIFSNI
                     VQSL"
     gene            complement(22035..22181)
                     /locus_tag="PKL11_02835"
     CDS             complement(22035..22181)
                     /locus_tag="PKL11_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNZ12565.1"
                     /translation="MLFAPKEKGQGLVEYALILVLVAVVVIVILALLGPIIGKIFSNI
                     VQSI"
     gene            22422..22943
                     /locus_tag="PKL11_02840"
     CDS             22422..22943
                     /locus_tag="PKL11_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12566.1"
                     /translation="MSDLFERVTGDQDFLTKLISKIPGFSGYVERQTRRASDKLLRET
                     IANRYEALWQRISALQVALINEGGILYVDDLESAALKLRQFIDRVRTASYGYAGFFDA
                     VKVNQEVLAKVYEYDLALLNSEDEMARAIDNVEASLGADGLPAAIRNLTTLAQQCVDA
                     FNKRAEVMLGGVE"
     gene            22985..24049
                     /locus_tag="PKL11_02845"
     CDS             22985..24049
                     /locus_tag="PKL11_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ12567.1"
                     /translation="MARIFDVVEYPSEMMDEIVHRFPEQGVADLRIGSQVIVRESQKA
                     VFFRDGHALDIFGPGRHTIVTANIPLLVDLVGKAFNDRTPFTAEVYYVSMREFADRKW
                     GTPQPILVRNPGMGLGVALLQAFGSYSFQVNDPQQFVTQLVGAQGMYRTSDIENRLRN
                     MLLSKLQDILGETGAKHSVAEMIGLTEEIGAGVRAKAQDDFKAIGLTLKSYYIANLKP
                     SEKSAEELRAMGMLDMQTYTQLQAADALRDAAQNQSGGAGLTAGIGAGIGLGGVINQS
                     LQGMTGAQAQPPAGGAAPAGSGTAAAIPAVMTPSEAAQILRVSEEDVVAAITAGDLKA
                     KKIGNAYRISKEALEEFLKG"
     gene            24128..25270
                     /gene="sucC"
                     /locus_tag="PKL11_02850"
     CDS             24128..25270
                     /gene="sucC"
                     /locus_tag="PKL11_02850"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNZ12568.1"
                     /translation="MKLHEYQSKHIFSRYDIPIPRGSVADTAEEARHIAEELGGRVIV
                     KAQVLVGGRGKAGGIRLAKSPDEAGDLARQILAMKIKDLPVRRVLLDEAVNIKKEIYL
                     GITNDRSSRRPVMMISAAGGVDIEEVARLTPEKIVKTHIDPLIGLREYQARDMAVAID
                     LPQDLWRSFTQIAAGLWRAYLENDATLAEINPLVITGDQHLLALDGKMILDDNALYRH
                     TDLAALRDVDEETSAESEARKNGLSYIQLDGQIGCMVNGAGLAMATMDIIKLYGGQPA
                     NFLDIGGGASAEKVAAAFQIILSDVNVKAILINIFGGITRCDEVARGILSAIRKARPR
                     TPMVVRLVGTNAVEGRRILAEANMITAETLVEAAQKAVALVGEVRT"
     gene            25267..26133
                     /gene="sucD"
                     /locus_tag="PKL11_02855"
     CDS             25267..26133
                     /gene="sucD"
                     /locus_tag="PKL11_02855"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNZ12569.1"
                     /translation="MSILVNKETRLLVQGITGREGLFHTEQMYLYGTNIVAGVTPGKG
                     GEWVLGGKVPVFDSVRLAVEVTEANTSVIFVPARFAADAIYEAADSGISLVVCITEGV
                     PVMDMLRVRRYLDQRHVRLIGPNCPGILTPGESKVGIIPGEIARPGGVGVVSRSGTLT
                     YEVLFALRQAGMGASTCVGIGGDPINGTSFTDILELFEADAHTDQVVLIGEIGGSDEE
                     RAADFIASRMTKPVAAFIAGQTAPPGKRMGHAGAIIERGSGSAEEKIRTLQASGVRVA
                     RHPEEIPTLFNH"
     gene            complement(26189..26443)
                     /locus_tag="PKL11_02860"
     CDS             complement(26189..26443)
                     /locus_tag="PKL11_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ12570.1"
                     /translation="MDDIFELVKAVIVDVLKVDGSEITRETRFIEDLKADSMDQFFLI
                     DGFCEKFGINIGDEDARAIRSVADVLTYLEAHRSELKIKG"
     gene            26656..27693
                     /gene="fni"
                     /locus_tag="PKL11_02865"
     CDS             26656..27693
                     /gene="fni"
                     /locus_tag="PKL11_02865"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNZ12571.1"
                     /translation="MDLPGISSRKADHIRINLEEDVASGLTTGLEDYHLVHQALPEIN
                     LSAVDLSQTLFGRRVQSPILISSMTGGTTRAASINQLLAQTAQQTGVALGLGSQRAAL
                     EHPDLAPSFHVRRFAPDILLLANLGAVQLNYGYSVDHCRRAVEMAEADALILHLNPLQ
                     EALQPEGETDFAGLLNKIEQVCSTLPVPVVIKEVGWGISEQAARQLANAGVAAIDVAG
                     AGGASWSQVEMYRLTDETRARVAAAFRSWGIPTAESIRAVRRVAPGVKVFASGGLRDG
                     IDLTKSLALGASLGGLASPFLKAAVQSEEAVLQLIEVLQGQIKIAMFACGALNLTALN
                     ESCLVPVKSAE"
     gene            27696..28730
                     /locus_tag="PKL11_02870"
     CDS             27696..28730
                     /locus_tag="PKL11_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ12572.1"
                     /translation="MPLSDWVTQMRPAVEETLRQIVDASFPPEHDSQSPLWAELRHML
                     TYHLGWDGPGSGPDAQGKRIRPLLVLLSAAAVDGSWQSALPGAAAVELLHNFSLLHDD
                     IQDQSETRRGRPTVWTRWGIPQAINAGDAMFTLVPLAINQLSSHHAPETVVAANRIVF
                     EASARLTIGQYLDISYEQARSLPLEAYWPMIEGKTAALLAACAEMGALLGGANAERQR
                     SFHAFGTSLGLAFQALDDILGIWGDETLTGKSATSDLVTGKKTLPVLYGLQQNGEFAR
                     RWLAGSIAAADAPELAALLEAEGAREYTQSRADRLTTEALSALNQASSPGDPADSLHQ
                     LTLSLLHRQQ"
     gene            28813..29391
                     /gene="folE"
                     /locus_tag="PKL11_02875"
     CDS             28813..29391
                     /gene="folE"
                     /locus_tag="PKL11_02875"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNZ12573.1"
                     /translation="MDLDTIQSSVRQILTAVGEDADRQGLQRTPERVARAYQELLSGY
                     RTDPLALVNDALFDVEYDEMVVVRDIEFYSLCEHHMLPFIGRAHVAYIPNGRVIGLSK
                     IPRIVDMFARRLQVQERMTRQIADLLCELLNPAGVGVVVEALHLCSMIRGVKKHDSRM
                     TTSTMFGVFRTDLALRMEFLDNISRSAEPLHF"
     gene            complement(29408..29884)
                     /gene="folK"
                     /locus_tag="PKL11_02880"
     CDS             complement(29408..29884)
                     /gene="folK"
                     /locus_tag="PKL11_02880"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNZ12574.1"
                     /translation="MHDAYLLFGSNINPVENLRRAVAELCTCCQLKNTSTAWETVAIG
                     SANAPNFLNLAAGMQTEVDARTLKEAVMRPLEARLGRVRTADKNAPRTLDIDIILFDG
                     VVLDEALWQRSFIAIPMAELLPDLIHPETGERLADLAARLLPGSGAIAHPEIKLCP"
     gene            complement(29888..30250)
                     /locus_tag="PKL11_02885"
     CDS             complement(29888..30250)
                     /locus_tag="PKL11_02885"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNZ12575.1"
                     /translation="MMDTVLIQDLLVRGVIGISDNERAQPQDILINVRMYTDIHAGAQ
                     SDDINQCVNYRTVAKKLFAHAEKVGRYTVEALAEDIARLCLEEPGVKGVWVRVEKPGA
                     VRFARSVGVEIERFNPAT"
     gene            complement(30284..31024)
                     /locus_tag="PKL11_02890"
     CDS             complement(30284..31024)
                     /locus_tag="PKL11_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12576.1"
                     /translation="MKKTQLLLENKTILITGAARRVGRLLALAAARGGADVVIHHAAS
                     PEAARQTASEVEALGRRAWVIQCDFSKPETAPALIEAAWDCAPLDALVNNAAIFEPLG
                     WADTGLADWQKHLDINLTTPFLLSQAFAKRLPVNGQGRIVNILDWRALRPGKDHLPYT
                     ISKAALAALTQALAQALAPRIAVNGLALGAMLPPADGASTDGVLQDVPMQRWAKPEEL
                     EEALIFLLSGPAYITGEIVHLDGGRHVV"
     gene            complement(30993..31754)
                     /locus_tag="PKL11_02895"
     CDS             complement(30993..31754)
                     /locus_tag="PKL11_02895"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ12577.1"
                     /translation="MFPYLEIGPLSIQSRGVILILGLWLALTLCDQLLKRARRTDIQG
                     EVIFNLAITGLIAGLIGARLAYAARNPAAFAAQPLALILPSGQMLDEEAGLLFGILAA
                     LIYGQRKKLPLWPTLDVLTPGLALMAIVIPLANLASGSGYGAPTTLPWGIRLWNEMRH
                     PSQVYEMLAGAITLALVWPRGGKQPAGQQFLRLMAWSAGWRLFLETWRGDSVMLTGSI
                     RLWQVVAWLGLALSLWLLGWLRRQSVEENTASPGE"
     gene            complement(31756..32304)
                     /locus_tag="PKL11_02900"
     CDS             complement(31756..32304)
                     /locus_tag="PKL11_02900"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ12578.1"
                     /translation="MDKPGRRALISLGLLALGALWMAATAIWFKPPAQAVAAPQVGFR
                     APDFMLTTSTGEQIRLSDLRGQVVLINVWASWCPPCKAEMPDLESVYAERRDEGFVVL
                     AVNATAGDTVEAAEAFFVEKGLTFPLLLDPESVVSEAYQVRLLPTSILIDSDGIVRGR
                     YTGAISAALLNSELDALLGGEE"
     gene            complement(32306..>33395)
                     /locus_tag="PKL11_02905"
     CDS             complement(32306..>33395)
                     /locus_tag="PKL11_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ12579.1"
                     /translation="VNKMKRLFVIIPALLLTIIFSACTPEPTLPAGPTPGPELIFLDW
                     AEDMPQSVLDQFEAEYGVTVKYLTYDATEEAAERIKIGDQLDVVVLDNDNIDALIRAG
                     MLHPIDPTAIPNIKNISPNFRGLAFDPTNTYSVPFNWGTTGIIYHTKRIDPPIRTWAD
                     LWNPELQGRVGLWLYEGRETIGFTLKSLGYSANSENPAELEAARQRLMELRPNVQFIE
                     NMSSGVTGVYLINEYQLDVAMGYAYDALEGEAVNPDIQYILPEDGVVLWSDSFVVPAS
                     SPNPVGAELFLNFILRPEIAAEIANHNYYATPNEAALSLLDPDLRNNPIVFPPAESLT
                     NAELLMPISPEAEALYNEIWAEFVNAAP"
BASE COUNT         7186 a        10170 c         8508 g         7431 t
ORIGIN      
        1 ggcgtcagcg cttccgtggg cagccgccag ctccagaatc cgcccgtctt gcccgggaag
       61 ttcatgcggg cttcggtgcc cagactcaac aggtcttgta tcggagccag agcttgcacc
      121 gccaccgaag accagatcgc ccgaatcata tcccaggcaa tatcatcccc cgagcgcgcc
      181 aggtagcggc ggcacagatc gcgctccact tcgggcgcgc tggcgtacca gcctgcaacc
      241 gtgtcattat cgtgcgtacc cgtataggca acgcagttat gcgggtaatt gtgcggcaaa
      301 aaggcatggg ttgcatcccc atcaaacccg aactgcaaaa tcttcatccc cggcaggttg
      361 aaactatcgc gcagctcaat cacatcaggc gtaatctcac ccaggtcttc agcgataatc
      421 ggcaggctgc ccagcgcctt ctcaattgca gccataaagt gcttgcccgg ccccggtacc
      481 cattgcccgt gttcggcagt agtgctgccg gcgggcactt cccagtagcc cgcaaacccg
      541 cgaaaatgat ccagccggat aatgtccacc atttccaaaa tggcgcggaa ccgctgaatc
      601 caccaggcgt acccggtttt gcggtgggct tcccacttgt acagcggatt gccccataac
      661 tgcccggtcg gtgagaagta atccggcggc actccggcaa ccgccgtcag gcggcggttc
      721 ttatccatat aaaacaattc cgggttggcc caggcatcgg cgctgtcaaa agccacaaag
      781 atcggaatat ccccgatgat ctggatgccc tgtttgtggg cataatcgtg cacagcccgc
      841 cactgccgga agaacaggaa ctggcgcagc ttctggcgcc ggatggcccc ggcattggcc
      901 gactggaacg ccgccagcgc ttcgggttgg cgcatccgga actcgggttc ccagttttcc
      961 cagcccacgc cgtgttgatt ttctttaatc gccataaaca gtgcaaaatc atccagccag
     1021 aaggcctgcg ccttctgaaa ttcctcaaat gcttccttga gcgcggcagg cgccgccgtc
     1081 tcaaaccgct tctcggcgcg ctccagcacc ttgagcttcc agggaatcac atcgccatat
     1141 tccacccgtt cagccgggaa cgctggccgg tcagccagat ccgcgcgggt cagcagcccg
     1201 tcttcccaca acagcgccgg gctgatcagg tacgggttgc ccgcaaatgc tgaaaagcac
     1261 tggtacggcg aatcgccata cccggttggc cccagcggca gaatctgcca aagtccgcag
     1321 ccgcagcccg ccagaaaatc aatccagcgg tagcactccg gtcccaggtc gccaatccca
     1381 tcgggccccg gcaggctggt ggggtgtaaa agaacgccag acgcgcgtgt aaattccatg
     1441 gaattatctc ccatcttgaa tgccaaagat gctttatttt acagcaaaac tcaactttcg
     1501 gaaaagccaa cttcggggcg gggcaggggc gggcgctgca gcagatccag gtaaagccgc
     1561 ccataggccc gggcagatgg ctgccaggag aaatcttccc gcataccgcg cagctgcatt
     1621 tcgcgccagg cgttgcggtt ggggtacagc gccagcgcgc gcagcaccgc caccgtgaac
     1681 gccgttggag aggcgtcgcc aaacaaaaag ccggtaccat cagcctcgcg gttatcccgg
     1741 attgtgtctt gcagaccgcc ggtttgccgc gccaccggca cacacccgta gcgcatggcg
     1801 atcatctgtg ccagcccgca gggttcgtaa cgcgatggca tcaacagcat atcgccgctg
     1861 gcatacagcc gccgcgccag tttcccatca aaacggatca ccgcccgcac ccggtctggc
     1921 agttccactt ccaggctgcg tgcagcagat tccaccagcg gatcgccagt gcccagcaga
     1981 atagcctgcc agggttggtc ggtcaccagc cgcagcccct caatcgccag gtccacgccc
     2041 ttttgattat ccattcgacc aatcataatc aacaacggcg catctggcag tgcagacagc
     2101 tccatctcct cgagcagggc ttgcttattc tttgcccttg cttccaaatt gtcggcgttg
     2161 aagttcgccg tcagggtggc atcagtcgcc ggatcccacg cgctgacctc cagcccattg
     2221 atgatgccag aaagggcctc ctggcgggtg ctcagaaaat cctgcaaccc gcagccaaag
     2281 gctggcgtca gcagctcccg ggcataattg ggcgaaaccg ccacaatgtg atccgccgtc
     2341 agcagcgcca gcggcagtgg gaacaggcgc gcccaatgcg gcagacgctc atcttgcgcc
     2401 ggaggaatgc cgtaggcggt cagcacaggc tcggcgcctg tgcccataaa aggcaggttg
     2461 tgaatggaaa taatcgagcg tgtctctgca aagaacggat cacttgcccg gcgcaaggcc
     2521 agcgcataga ccgcggtggc ggtatgccag tcattggcgt gtaaaatatc cggcttccag
     2581 ttcagctttt tcaccacctc cagcgctgcc agcgagaaga acacatattt ctcgccatct
     2641 tcccgcgaat tctgagtgtt atacaccggc gcattaggtg ggatgggcgc tccttcaatt
     2701 aaatagaccg tcaccccctg cactttggtc tcaaatgccc gtccggcaat ctcacccgtg
     2761 gcgtgtggca ccgtgaaaga ggccaccagt tgtatctctg gcagtttttg ggtgatggct
     2821 gaatggaaag gcagcgccag ccggatatcc ggctggcgtc catccaggtc ggctggcagc
     2881 agcgcttgca gcgcccgcgg cagcgacccg gccacatcgc ccagtccacc caccttcacc
     2941 agcgggtcag cttctgaagc gataaacaat acacgcagtg gggtggggtt tgctttcatc
     3001 ggatcatgtc cagataatct tcactggtcg cttcctcagt ggtagcgccc agcagttcaa
     3061 tcaactcaac cgccagccgt gatttatctt tggcgtcctg ccggctccag gttcggctct
     3121 tgatttcaag atagtaaccc agtgctggtc gctgcacctg atcaatatta atgaagaact
     3181 caatcccttc atactgcacc aggaaacgca gccggtcttt ctcaatttcc atttccatgg
     3241 ctggcttgaa gtattctcga tagaatcgtg cggtctgcgc cgcaggtgcc aggaagcggc
     3301 tgcgtgagag cagcaccttc tgcgagtact gcgcttcgcg cgccgggcca atatgcgtca
     3361 gccgcgagcg gacgttggtt atctcgccct tgtcactgat aaagtgatcc tcgcggtacc
     3421 gcaggtatcc ctggtcgggg tcggtaaagc agaaataagt atcatactgc cggtagtgtc
     3481 gcttgcgtac aacactaatc gccgggtttt gcagggcagc caaaacgggt tgagcatccg
     3541 gaatgcgcac cttaatttgg acttcgtagc tctcttcctc ggttgccccc ccgattgcca
     3601 ccagttttga caggatggat ttttcgcgct ggaacaggaa cgcatcaatc cgtgtggcgt
     3661 actcctggct gtgatgcagc agttcatttt catccagggt cagcagctgc cgttcccgca
     3721 tcaaccactt gccattcacc atcacatcgc tcacgtctgt tgacttggcc gcgtacacaa
     3781 tctgggcata aatgcctgcc gggtcacggc ggaaccgcgg catattgtgc gccggagaaa
     3841 tatctaccag aatcaagtcg gcgcgcttgc ccggctccag cgaaccagtc agactgccca
     3901 ggtgcagtgc ctgcgcgccc agccgggtgc ccatcaacaa aacagtcgag gccggcagcg
     3961 cggtcgggtc gccgctggta cccttggcaa taaaagccgc cagccgcatt tcttcaaaca
     4021 tatccaggtc attattcgat gccgggccat cggtgccaat gcccacattg agccccaggt
     4081 caatcatgcg ccggattggc gccacgcccg aggccagctt gaggttggag gaggggttgt
     4141 gcgccacccc cgcccctgca tgcttaagcg tccgcatttc gccttcatca atatgaacac
     4201 agtgagccgc cagtactttg gcatcaaaca ggttctgctt cttgacgtag gggatcactg
     4261 gcattccctg ttcgttgcgc atggtctcca cttccgaagc cgtctcgctg agatgggtat
     4321 gcagtggcac gtcaaactct gccgccagcg ccgcacaggc ctgcaagatc tcgggggtgc
     4381 aggtataggg cgcatggggc gctaccgatg gcacaatcag ggggtgattc ttccaccgca
     4441 caatgaaatc ccgtgtggct gccagtgagt cttcgtaaaa ctgtgcatcc ggcgtcggga
     4501 atttcaacac agtttgtgag cacaccgccc gcatgccagc ctctgcagtt gctttggcaa
     4561 tatcttcttc atagtaatac atgtccgcaa agcaggtagt cccgctgcgg atcatttcgg
     4621 cgcaggccag ttggctgccc agccgcacaa aatccggcga gacaaactcc cgctccactg
     4681 gcatcatata gcccagcagc cacacgtcca gccgcaggtc atcagccaac ccgcgcagca
     4741 gcgtcatggg aatatgggta tgggcgttca ccagtcccgg catcagcacc ttgccgccgc
     4801 aatccaattc ctcgctggcg gtaaaatgct cacgcatctc gcccatgggg ccaaccgcca
     4861 caatctgatc gccgcgtaca gccaccgctc cgggatcata tatatgcatc agttcgtcca
     4921 tcgtcacaac gatggcattg gtcagaatca ggtctacggt ttccatattg tttttatcct
     4981 tcactggtaa taatccgccg caggaaatcc agggcagtgt tggcgctcca ggtaatactc
     5041 atgctcgccg gaccgcccca cgagcgttgc agtgtatggg tttcagatgg gcctgctgca
     5101 atcagcagca aatcctgctg ccattgcccg ggcgtcaggt tggcagccag cgcaatgtcc
     5161 acaggttgtt cagcctggag cttgcgcaga tgctcccgca gttcttcctc cgtgcaaggt
     5221 gattcaatcg tccgaatgca gcactctgcc agtccggcag ccttaagccg cttgctcaat
     5281 tcatcgccta agccacattc aatcactgcc agcgtcagcg cgctggattt tgcccgtgtc
     5341 atcaccacgc tttccagcgt atcggtatcc tgcccaaaga tcgcctcacc cagccgccgc
     5401 cggatcaaaa tggcgtattc gtgcagcagt tggttggcct gctcaagcga ttcagccttg
     5461 gcggtgatgc gaatatcaat ctgcccaccc tttgccagca gccccaccgt tgggttgcgc
     5521 agggtttcca ggtcgccaat caattcatcc acctgtgatt cgccaatccc cgaggcgtgc
     5581 agcaccagcg ctttgatgat gccgcgcagc ccatatcgtt ctttcaagaa cggaaagatc
     5641 acattttcca tcaaatattc catctcgcgc ggcacacccg gcaggctgat aatgacgcga
     5701 tcgccggtat cataaataaa cgccggggcg gtccctacca gattctccac cgcctgcgcc
     5761 ccctgtggaa tataggcctg acggcggtta ttttctgtcg ccagccgacc aaaccgctta
     5821 aaccgatctt gaatttgcag ccacaaatcc tcacgatatt ccagcggcac acccaccgcc
     5881 atggcaaccg cttgccgggt ggggtcatct accgtcggcc ccagcccgcc agtggtgata
     5941 attatttcgg ctcgtcccat acattcctga atggtttggg caatccgctc ggcattgtcg
     6001 cccagcatgg tggttcgata taggtcgatt cccacgtcgc gcaaattgcg cgccaggtat
     6061 tgcgtgttgg tatcttgaat ttcgcccaga agcagttcgg tgccaatggc gatgatttca
     6121 catgagggca taggttttct ccaataatca gggattagtt caacagcgtt acttgctctt
     6181 cggctcgtcc tcgacaactt gatagagcat ggccgcacgt atctgcaaat cagcgccgcg
     6241 aattttttgt gccagttcgg ttgccaggtt aaacatcacc cggtagccca gttcgggata
     6301 ggaattacat aacagcagga acttgccgcg gggaatccgc agcacctggg tgcctttttc
     6361 caaagccaca gcgctggcgg tacgtacacc ctggtccacc agagccacct cgccaaaaca
     6421 ctgcccgcgc cgcagcacgg caattgtcgc aggttgcgcc gccgccttgg ggtcagccgc
     6481 caccagcgta gggtccattg taatctgtac ttcgccttgc agaatcaggt acagctcatc
     6541 ttcgcgcgaa ttttcttcaa aaattttctc gccttttttg tacacgcgct cttcacaaat
     6601 actgtccatc aattccaact gcgtggcggt cagattgtag aatagttcgg tttctttaag
     6661 aaaaacagca tacttgctca tacagccctc cgggtgtgaa gtaagtttag cattaaaacg
     6721 gttgccagac aagttgaaat ttcgcttctt tctggaacca acaatgccga atccggattg
     6781 gctcaactgc aaaatcaacc cataaatcgg ttacaatcgg gccatgcctg aaaaccacaa
     6841 gcagcagctt ggtcattggg gcgaacagat cgccgcgcac catcttatgg agcacggact
     6901 caccgttatc gcccgcaacg tccgtacccc ctatggcgag ctggatctgc ttgcccggga
     6961 tggcgaggtg ctggttgtag ttgaagtcaa aacccgtgcc agtgcaaact ttggcctgcc
     7021 cgaggaagcc atcaccccgc aaaaacgcac ccacctgctt caatcggcgc agcactattt
     7081 gcagcagcac cccgaattgg acggcgattg gcggattgat gtcattgcta tccgccgtca
     7141 gccgggttct caatctgtgg agattatctg gtttaaagat gctgtttctc aatagccctg
     7201 cccccttatt agtgcttgtt ggccccaccg ccgttggtaa aacggagctt tcgcttgagc
     7261 tggcggaacg cctcaatggc gagatcatct ctgccgattc gcgcctcttt taccgcggta
     7321 tggatattgg caccgccaag cccaccctgg cggagcgcca gcgggttccc caccatctca
     7381 ttgatgtcac tgaccctgcc gataactgga ccctgaccat cttccaggcc gcggcggctc
     7441 aggccatcgc cgaggtccag gcccgcggcc gtttgccatt gttggtgggg ggcaccgggc
     7501 agtatgtctg ggcggttatt cagggctggg cgccgcccga acaggacccc gatcccgcct
     7561 tgcgcgatat ccttgaacgc tggggacgtc agattggccc cgaagaactg catcgccgtc
     7621 tgcgccgcat tgaccccgcc gccgctgcgc ggattgaatc gcgcaatctg cgccgcaccg
     7681 tccgcgcgct ggaggtgatt ttcaccaccg ggcagcgctt ctctgcccag cagcgccgta
     7741 ctggcagccc ctacaccacc cgcatcatcg gtttgcagcg cccgcgcccc gagctatacg
     7801 cccgcatcga tgcccgcatt gatgccatgc tggctgctgg tctggtgcag gaagttcaag
     7861 ccctgctgga gggcggctgc ccaccaaacg ctccagccct ctctgcgatt ggctatcgcg
     7921 aaatcggcca gtatttacag ggtaaaatca ccctccctga agccattgtc gaaatgaaac
     7981 gcctcacccg tcagtttgtg cgccgccagg gcaactggtt caagaccgat gacccccaga
     8041 ttgcctggtt tgatgctgcc agcatcaccg cggctgaact tgagacgcaa attcgttcct
     8101 ggacagtctg atattattgt tactggatgg aaacatggaa aaaccctggt tacagcatta
     8161 tgatgctggt gttccggcgt cattaaatta cccaccacaa accgttcacg gcattttgga
     8221 agccaccgcc gcctgttggg gtagccagcc tgctacctgc tttgccgacc gcttcctttc
     8281 gtatagccag ataaacgccc tggcggacca gcttgcccgt gcccttttaa tccttggatt
     8341 gcaaaaaggc gaccgggttg gcctgttgtt gcccaattgc ccgcagttcg ttctggcctt
     8401 ttatgccatc ctcaaagccg gtggggtggt ggctgccatc aacccgcagt accgcccgcg
     8461 tgagctggaa gcccagcttc aagatgccgg tgccgagatt ctgatcagcg tccgttctgc
     8521 tgctcaggtg atccagtctg ttcagccgca cctcaacctg cgccacatca tttggaccga
     8581 tctggaagac gcccctgccc tcgctgaccg gccttcttct ccgcccgcgt tttaccccgc
     8641 ccccgagatg gattttctct ccattctgca ggcccctctt ctgccggcag cgctgcccgt
     8701 catcacccca gaagatgttg ccgtactgca atataccggt ggaaccactg gcacccccaa
     8761 aggcgccgtt gggttgcatc gcaacctggc agccaatacc cttcaatttc gctgctggct
     8821 gtctggcctg accgatggcc gcgaagttgc tcttgtggct atccccctgt ttcatgttta
     8881 cggcatggtc attggcatga gcgtcagcgt tgccttgggc gccagcatgg tgctgctccc
     8941 caatggccgt gatattaccg ccatccttca gcaaattgaa caataccatg ccaccctgtt
     9001 ccccggggtt ccggcgctct atcatgccgt taatcaacac cccgatgtcc ttgccgggcg
     9061 ctacaatttg cattcggtca aagcgtgcat ctccggttcg gcgccgctgc ccgtcacagt
     9121 caaggccgag tttgagcagc ttaccggcgg caaagtcatg gagggctatg gcctctccga
     9181 agcccccacg gccactcact gcaatcccat gctgggtgag aaccgccccg gctccattgg
     9241 cctgcccctg cccgatgttg attgtcgcat cgtctcaatg gccgatggtc aaacccctct
     9301 cgcagttggc gaagctggag aattgctgat tcgcggcccc caggtgatgc aggggtacca
     9361 caaccagcca tccgaaacag ccgccgcact cgcggatggc tggctgcaca ccggcgatat
     9421 tacccgcatg gatgccgatg gttatttcta cctggttggt cgtaaaaaag aactgattaa
     9481 agtcagcggc tatcaggtct ggcctgctga agtcgaatct gccatcatca cccatccagc
     9541 catcaaagag gtgggggttg ccggtgtccc cgatgaggcg cgcggcgaag ccgtcaaagc
     9601 ctgggttgtg ctccaccccg gtcaatcggc cactgctgat gaaattcgcc agtggtgtct
     9661 ggaccagttg gcgcactaca aggccccgcg tgaggtcatc tttgtcagcg ctctgccgcg
     9721 taccccggtt ggcaagctcc tgcggcgcga gctgattaac ctcacggctt gatgcacgcc
     9781 actcacccga atcaaaaatc ccgcagatga tcaatctgcg ggattttgct tgtctagttc
     9841 atgttcatcg ccgggatgcc gcttttcttg ggcaccggcg gtgggaagat ggcctcaaac
     9901 tcttcgcgag acagtgtttc cacttccagc agtttgtttg caatggcgtc cagttcctgg
     9961 cggtactggg tcaggatccg gcgggcttca tcataagctt cactaaccag ccggcgaact
    10021 tcctggtcaa tccgttcagc cacagcctca gagtagtcgc gctgctccga aatctcacgg
    10081 cccaggaaga tcagctcttc cttctgcccg tagatcatcg gcccaagctc gctgctcatc
    10141 cccaatcggg tgaccatgct gcgcgcaacc tgggtcacgc gctcaatatc atttgccgca
    10201 cccgaggtga tatcgtcaaa aacaatttcc tcggcagccc ggccgcccag cagcgtgacc
    10261 atctcagcca gcagcttctt gcggggcatc agaatccggt cttcgcgcgg cagcgacagg
    10321 gtgtaaccgc cagccatgcc gcgggcaatg attgagactt tatgcaccgg gtcgctttcc
    10381 ggcagcacgt tcataacaat ggcatggccc gcttcgtggt aggccacaat ccgcttttct
    10441 tcggggttaa tcaggcggct cttgcgttct ggtccggcaa tcacccgctc aatggattct
    10501 tcgaattcgc tcatcccaat cgagcgctta ttgcgccgcg ccgccagaat ggctgcctcg
    10561 ttcaccaggt tttccaggtc agcacccaca aaaccggggg tcgagcgggc aatcacgccc
    10621 agatcaacgg ctggctcaat cggcttgccg cgcgaatgca ccttgaggat ggcttcgcgc
    10681 ccgcgcatat ccggccgatc cagcaccacg cgccggtcaa agcggcccgg gcgcagcaac
    10741 gccgggtcca gaatatcagg gcggttcgtc gccgccatga tgatgatatt ggtatcggtg
    10801 tcaaaaccgt ccatctcaac cagcagttga ttgagcgttt gctcgcgttc atcatggctg
    10861 ccgcccagtc cagcgccgcg ctgacggcca accgcatcaa tttcgtcaac aaagacaata
    10921 cagggcgaat gccgcttggc ctggtcaaac aggtcacgca cccggctggc gcccacaccc
    10981 acaaacattt ccacaaactc cgaaccagag atggcgaaga agggcacccc ggcctcaccc
    11041 gatacggctt tggctaacaa tgtcttgccg gttccaggcg ggccaaccag cagcacaccc
    11101 tttggaattc gagcgcccaa ctgcaagaat ttctgcggct cgcgcaggaa ctcaaccact
    11161 tcttgcaggt cttctttaga ctcatccacc cccgcaacat cggcaaacgt cacggtgggg
    11221 tggtctccgg tgaacatccg ggcgcgtgat ttgctgaatg agattgccgc attattactg
    11281 ccctgcgcct gccggaagat aaagaagaac acacccacca tcagcagcaa gggcagcaca
    11341 attgccaaag ctgtcaccac attggcgcgc acacttgggt ttttgatgac gatcttcacc
    11401 ttatccggcg ccagatcttc agaggttacc ccaaactgat agagttgctc aaccagcgtt
    11461 gctccgggct ctttgaccag cttgttctct ttaccatcgt tataggtaac aataagatta
    11521 ttgtcatctt cttcaatgac tttgacctta ccgtcttgaa tatccagggc cagttggttg
    11581 atcgtcaatt cttcagctgt gttattgcgg ttaaacccca cataaatcat cgtcgcaatc
    11641 gcgatgaaca gcagaatata gatcagcact gatgagttac gttgattcac tgctacctcc
    11701 aggtagttat ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatagatca
    11821 gcactgatga gttacgttga ttcactgcta cctccaggta gttatccatc cgttcccttg
    11881 ggggaaccca atgaaaacat tataccaatc cagtgccccg acttccagca attggacagg
    11941 tatttgctcg ttttttacaa agttcttata aaaacgatcc aatttatggg cgcacagcag
    12001 cttcaatggc ctggcggcac tgccgaatat gattttgatc atgggttgcc ataaacccta
    12061 ccagttccag cagccgggtt ggtccaaata tggcatggcg ggctgttcgc tgccacgcgt
    12121 ctgccggcag gctgtccagc aaatcaagca gttcgcatcg tacagcaacc gcttctctta
    12181 acgctgttgg gccatcttcg ccggcataat gacgttctgt cacccagcca tctgccgaaa
    12241 cccccggcaa gaatgggttg gattcttcac cgctgctcat gagccgcact cggggcaggt
    12301 tgatctcccg atccacatcg cgcaagtgcc caataatctc aaccagtgac cactcttctg
    12361 ccgccgggcg gtgcgtccac acagcctcgg gcagtgcttc gcataacgca tccagcgccg
    12421 ccggggttga atgaagcgct gcccggagcg ccaccggagt atcccaggca gtggcatgcc
    12481 cgtgcgcttc cacatcttcc agccagggca gaatcgcttc cagcgggcca ccaccattca
    12541 gcttttcggg ctgcagcaac caggtatgca gccccagcgc tgccgctggt tcaatatcat
    12601 ctgccgcatc gttgcctacc atcaccaccg gtccctcggg ccagcccaac tgagccaaca
    12661 tttcagcata ataggctggt tggggtttgg caaagtggaa ccgctcatag gttgaaacca
    12721 gcgcaaaatc cgaactgtcc atctcggccc atttgatgcg ctgctcggtt gccactcgcg
    12781 ggaacagcgg attggtggca attgccaccg tccaaccccg ccgccgggct tctgccacca
    12841 gttcaatggc tgcggatcgc ggtttggtat attgccgaat ctcggggaag accgtggtat
    12901 aaaacccata aatcatgtcg ctgagctggg ctttttccag ccccagcatg gggtaaaagt
    12961 ggcggtcaaa ggtttcttcc agtgtaccca ccggggtttg tttttgggtc atctgttcgg
    13021 tggcaaacag cagttgcgga atcatccgtt ctgccggaac aaccgcattg aggtgcgcac
    13081 ccagcgcctt caaataggct ttctgaaacg cccccattgg gttgtcaaat aatgtgtcgt
    13141 ccagatcgag gagtaaggtt aaactcatga tttctcggtg aaaataggcg gcagtggatg
    13201 gcattgcccg cagccaatct gcggctcggc aaccggctgc agggttttcg tgcaggtcgc
    13261 cgtcacctta acgtgccggc caatccccaa aaagccccgt ttcacttccc cttccaatac
    13321 catattgggg caagaattgg cgcgtaaaat cccgggcacc gggcaggtct tgcataattc
    13381 aggcttccaa tcccgcacaa gcaaacggca ttcctcggtg ttcctgccgc ggtaataatt
    13441 gccataaaaa taacgacatt cagagccagc cggagtacgc aagctaaaac ctccttttat
    13501 caaaaggctg ttcccacagg gaacagcctt aatcgccttc tgtgctaaat ttcagccctc
    13561 tagttggtca cgcgggtcac atagctgccc gtgcgggtat ctacacggat gacatcgccc
    13621 tgattgacga agttcggcac gtccaccgag gcgccagttt caaccttgac gcgcttggtc
    13681 acgccagttg ccgtatcacc gcgcacagcc acttccgctt ccacaacctt caaatcaacc
    13741 gaagtcggca gttcaatatc aatcgcttca gagaagtaga aggtcagctt cacttccatc
    13801 tcttcccgca agaaaccaat ggcatcttca atgatcccgc ggtgaatggt gtactgctca
    13861 taggtctcag tatccataaa gtagtacaga tccccatcat tgtaaagata ctgtgcattg
    13921 tggaaatcca accgcacatc ctgcacccgc tcccccgact ggaaggtctt ttccagcgtg
    13981 gtgccgctgc gcaaattgcg tgcttttact ttgatggtgg cgttgccgcg cccgggttta
    14041 ttgtggctgt attccaggac tttgaacaga ttgccatcta gttcaaaagt cacacctttg
    14101 cgaagatcat taacatcaat catggtcatt cctatctgga tctaaaaggg tcttaaaaac
    14161 gaaacagggc tgattatagt acagtgtgag aagggtgaca agagcagcaa acaaaagcag
    14221 ggcggctcaa gccgccctgc cacaagccaa agtcctaaat ttttagccga tataaggctc
    14281 aatcaacccg tagctgccgt cgcgccgccg atacagcaca ttgatcgcat tggtgttgac
    14341 attgaagaag ataaagaagt tctcatgccc caccagcgtc atctgctcaa gcgcttccag
    14401 ttcatccatc ggagaaagcg aaaacttctt ccggcgggca atcacgggca actcaatctc
    14461 atcttcaacc tttaccggcg caacttccga ggccggtgtg ccatcgccgc gcccgtgctg
    14521 ccgcttgccc ttcaagcgtt caatctggcg ctgcatctta tccaccgcgg catcaatcgc
    14581 agcaaaaata tcctccgcgc gctcttccgt gcgcaggatg aaccgtttgc cgcgcagcgt
    14641 tacctgggcc acatgccggt ctgctgcatt acgagccgat ttgacgtgtg agaggtccac
    14701 ccgcgcctca tcaatatcgc ccaaaaaacg atccagcttg gatactttcc gaaccgcata
    14761 atcgttcaag cggtcggtgg cttccatatt ccgaacaatc acgtcaactt tcaggtccat
    14821 cgcatttacc tcctggagaa ttggggtgta tgggttccgg agcgtgcacc cgtttgctaa
    14881 cccgggtgga actcatccgg tcaggctgtc tggatgattt gttaatccgg ctcgcgccag
    14941 tgtcagtgca tagatgttgt gagcgccagc cgcttgcagg gcctcaccgg ctgctaacat
    15001 cgtggcgccg gttgtagcca catcatctat caacaacacc gctcgcccgg ctacctgccg
    15061 tgcatctgct gtaaaggcgc cgcgcacgtt ctgacgacgc tcgctgccgg tcagtttcac
    15121 ctggcttgcc gtgtctcgtg tgcgccgcag tgcctgggcg cacagtggaa gtcgggttgc
    15181 caggctcaaa ggccttgcca gcaggctact ttgattatac ccgcgttcgc gttttcgttg
    15241 cgcactgaga gggatgggaa taatcatctc aatctgccag tcaagacttt gccacatggc
    15301 aacaagtttt gcgcccaacg cctcacccag tcccaggtcg ccctggtatt tcaaccggtg
    15361 aatggccttg cgcagtgcgc cagcataaac cgcccaggaa cgcagcgccg tgtagggcgg
    15421 gggttgggcg gcgcaatctg tacagatctc gccgccgtgg ttgggcaccc cgcagtgtgg
    15481 gcagatagcc atattttcca gcaatacggt ttgctcctgg cattttgcac accagcgttc
    15541 gccgggggca tcacagccac cgcaaacagg ggggtataac caatcagccg cttgccagag
    15601 caattggtga atgacgtacc ggggtgggcg tttggcctga cccacaaacg tcatacggcc
    15661 tcctgatcta ttgcatgaaa ccttgtacca gccggatacc agccggggtg agcagaacaa
    15721 tcatcaagga tgggaaaatg cagccgccca tcggcagcag catcttgatt ggcgctttat
    15781 gggcctcttc ttccgccagt tggcggcgct tggtacgcat ctggtctgcc tgaattcgca
    15841 gaaccttaga catactcaca cccagctgct cgctttggat caccgccgcc acaaaactgg
    15901 tcatttctgg aatgccaatc cggtcggcca tatcacgcag cgactcgcgg cgggtcttac
    15961 ccagttgaat ttcccgaatg cagcgggcaa acgccagcga aagctctgtc tgccatttct
    16021 cgctcacttt agccagcgcc gcatcaaacc ccaggccggc ctcaacgcag attgtcagca
    16081 ggtccagcgc atctggcata gccttgcgaa tttctttctg ccggcggccc accttgctcg
    16141 tcaaatacaa ctgcggaaaa taaaaaccta cggctgcgat accaatcgtc aatagaatga
    16201 tccgggtaat tggccagact gttgagccaa ccgagaacac cagaatcaca atcccggcaa
    16261 atactgccgc cacaataaac tgggctgcca gaatcattgt cgcatctagc cctgccgggc
    16321 tgccagccag ctccagcttc cgcgaaattg attgcagcat tttctgtggt gtaaagcgga
    16381 tggcaatctc acccagttta cgggccatgg ggtaaatgac gcgttgggtg aacggctgcg
    16441 acagctccag cttccgcaaa tcaacttctt cgcctgccgc gctaaattct tccaggcggg
    16501 ctaataattc acgatcttgt tcctcggcct gggggcgccg gaggccaaca attgtcagcg
    16561 taactgcacc gccaacgagc agaacaccca ggataatgat aattgcagta cccatattta
    16621 atctccttag atgcccttac acttcaatat ccgcaatttt ggtcatgatc agatagcccg
    16681 acacgattaa agtggccgcc aggcccaggg cgatataccc aataatcccg gatttttcat
    16741 caaaaaatgt cattatgtaa tcccggttca gtaaatatag aatgccgatc acaaacagcg
    16801 gcatcatcgc caggaactta ccggaataca tcacctgcga caccatcacg cgaatttcgc
    16861 ctttaatccg cacccgttct cggatagtat atgaaatcgt gtccaaaatt tcggccaggt
    16921 taccacccac ctcgcgctgc acgttaatcg ccgtgatcac caggtccagg tcgtcagatg
    16981 ggatgcgtcc caacaggtta gccagcgacc gttccatcgg cacgcccaac tgcatttcct
    17041 gcaccactcg cataaattcg tcagaaatag ggggcggcag ttcccggctc accgcttcca
    17101 tggcttgcat ggtcgaaaaa cccgcccgca gaccgttcac catcaggttg agcatgtcgg
    17161 ccagttgatc gttgaattta atcagacgct tcttttgctg cgatttgaca tactgccccg
    17221 ggtaccacag cccaatgacg ccgccagcca gcgccccaaa aatggattgc ccgctgataa
    17281 catagcctaa tcctgctaga ccgatgattg cgatgattat taccgcaata tattcgcccg
    17341 gtttgatttt caagtcagcc cgggccaggt ccgtggccat cttggagcca aaggccgatt
    17401 tctcaacgcg tttattcaac cagctggtta aaggcgtctt ggccagggct ttttggcgtt
    17461 cgtcaacttc ttcctgggtc gttacatact tctccatccg gtcatcaacg aaatcgcgct
    17521 cgcttctcgt tgcaataacc acgccgataa tcagcgccac aattacaatg ccgccgacaa
    17581 ccagcaagat ggtgagtgga ggaataccgg ccattttatt ctgcctttcc ctttaatcgg
    17641 tgcataaagc ctaccgccgc cgatcgccaa tcccaaagat gtttggaccc agcttgatgc
    17701 cggcagcttc aatccggtcc atgaatttag gccgcagccc gctggcgcgc aatcgtccct
    17761 ccaccttacc ggtgttggga ttgagaccag tttgttcaaa gaagaacagg tctgtcagag
    17821 tgatgacttc accttccatg ccggtcactt cgctgatgta ggtcacctta cgagtgccat
    17881 cccgcatacg ttcctggtga atgacgagat caatagccga agaaacctgc tcgcgaatgg
    17941 cgcgggctgg cagatccatc cccgccatca tcgtcatcgt ttccagacgg gagagggtat
    18001 cgcgcggact gttagagtgg gccgttgtca tagagccatc atggcctgtg ttcatcgctt
    18061 gcagcatatc cagcgcagcc tcatcgcgaa tttcgccaac cacaatccgg tctgggcgca
    18121 tgcgcaatgc attgaccacc agctgccgga tggtgatttc gccgcggcct tcaatattcg
    18181 ccgggcggga ttccagcgtc accacgtgtt cctggcgcag ctgcagttca gccgcgtttt
    18241 caatcgtcac aatacgttcg tcatctggaa tataacccga aagcacgttc aaaagcgtgg
    18301 tcttaccaga acccgtaccg cccgagatga tgatattgat gcgcgcaatg actgccgccc
    18361 gcaaaaactc aaccgcctca gccgtgatac tgccgtaatc aaccatctgc tcaatcgaaa
    18421 tgggtttctt ggagaacttg cggatggtaa tcacaggccc aaccagcgaa atggggggga
    18481 taaccgcatt cacgcgcgag ccgtcaggca aacgggcatc cacatacggg ctggattcgt
    18541 caatgcggcg gcccagcggc gccacaattc tttcgataat gcgcatgaca tgctcattgc
    18601 tctcaaaaga cattggcact ttgaacaact tgcctttgcg ctccacgtag atatttttgt
    18661 acccgttcac catgatttca gtggtgctgt cgtcgtccag cagtgccgaa agtgggccaa
    18721 aacccagaat ctcagccaca atctgttcga acatgcggtt tcgttctggt cgtgagagta
    18781 cgatattctc ttcatccaaa atctgttcaa acagttccag aatcgcccgc cgcacttcat
    18841 ccacccggct cacatccatt tgcggattga gggttgtaag cagcttggtc tgcacccggt
    18901 tcttaatatc ggtgtattta tcctgctgcc cgcccatcga gggcatcggc acccgcgcgg
    18961 gtggcatgga cgatgagccc ggggtttgtg agcccgattg tccagaatcg ccttcgccag
    19021 taattctgcg taagatcgac atgcgctcga tttcctttga ttaatttact tgcgccggcc
    19081 gtcagatgag ccttcttgcc cggctttggg tttgttgatg tgttcctgca caaggtcagc
    19141 cagtgcctga atgctcttgc tgatgggcag tgtcttgtta tctacaaaga atgatatgcc
    19201 ccggttaagg taatagctga ccttctcttc cagcggcagc accagcataa taggctggcg
    19261 caggctttcg cccacctttt caggtgggat cgtgatgcgt ttatcgtacc ggttcatgac
    19321 gaagatgatc gaatcgcgcg gaatccgcat cagatcaatc agcgacagga acaggttgca
    19381 gttcttgatc gccgggaatt cttgcgtcgt caccagaatg gtaatatccg cattattcag
    19441 cgcttccagc accggttcac tgaggtaaga gctggtgtcc accacaatcc agctatacat
    19501 ggattgcagg aaccgcagca cttttccaaa ttgctctccg ttgaccttct ctgccatttc
    19561 ggggcggggt ggggccgcca gcatactgac ccccgtggtg ttctgcacca ccatcacctc
    19621 gcgcaccacg tcggcatcca tttcatccac ccggggcgcc agatccagga ttgtattctt
    19681 gccctgttca ttcaacgcca cggccacatc gccaaattgc agactgccat caatgacagc
    19741 agccggggca tccggcttgt gcaaggcaat cgccaggttg gtggcgatgg tcgtgcagcc
    19801 agtaccgccc ttggggctgt aaaccacaat cacgcgtccc ttatgttgtg ctgaccccgc
    19861 catcattcca gccagcgtgg gcatgccgcc gggggctgcc gtataggctg gcggcgcagc
    19921 tttcgaccgt tcttcgtgcg ccaccactgc cgcctgccgg atggctgatg tcagttcatc
    19981 aatcatcggc ggtttggata gaaaatcgcg cgccccggcc agcatggctc gccgcatata
    20041 gctggagtca ttctgtaccg acaggataat cacctgcgca aacggcactt tgcgacggat
    20101 attctccgtc gcagtgatgc cgtccatatc gggcatgttg atatccatca ccaccacatc
    20161 gggtaggagt tgctgcgcca tttcaatggc ctcgcggcca gtgcgcgccg cccccacaac
    20221 ctcaatattg gtgtcaaact gcaacatccg gcgaatgttt tcgcgcgttt cgttaatgtc
    20281 gtctacaatc agaaccttaa tcttttcggg agctggcatg gattctccgg taagtaatca
    20341 gattacctgc ctgaaaaata atctaatctt caggcagcgg catgggtaca gtctcaacgc
    20401 cgtaaggtag cttggcaggc agtggaatca cgctctgatc cattaagtat tgttgagtta
    20461 ctgcgtcggt caggtagatg gtcgaatcag tggggttgcg catcgccagg ctgatgcgaa
    20521 taccagaaag catcatgtaa tttaatcgca gtgcgtcctg cggcggcatc atcagggtag
    20581 ccagggtcgg cgcaattgct ggcggcgggg cggtttcttc gccgccttca gcattggttt
    20641 ctgcggctgc cgcctgcgcg gccgcatcat caaagtagct gccaatcttt aacacaatcg
    20701 catcctggat aatgttctgg caaaccagcc gcgggcgctg tgtctcggag ggttgtacat
    20761 aaaacttaaa accctgcaaa tcttcaacac gtccgattgg ttggtattgg tattctgtcc
    20821 catctgcctt gatgttgaat tcattcccta aaacactatc ttcctcggtg ccgctattta
    20881 ctacagctgt aacagggaga atcgctcttc cttcatctgc attaactgat gtcgtctctg
    20941 caagaaaccc agcggcatta ggcagtgggg tttgaaactc aacatcataa tctaaaatcc
    21001 acatacagcc tatcaccatc acatgatcgc ctggctgcag cgcataggct accgctgtca
    21061 gatcattaat cggcatcgaa agcgccgtca tgcccaccgg tacatcaaat gaaggggacc
    21121 aggcgttccc gccggcatcg accagcatct ccatggtcag cggcatgcga gccttcaaat
    21181 cataaaccgc ggtcaatccc agcagttctt ccttcttgat gtcttcgctg tctgccacaa
    21241 taaagatgcc gccgctggct tccagttcgg cgcgcgggta ttgaatggta ccaaggttag
    21301 cctcggtcaa tacttcaccg cgcctcacat cctgcagcgt gatgaagata tccaccaggt
    21361 ctgttggcgg gggagctgta ggctccacct caccgctggg tcctacccca agcccaccgt
    21421 tgtttgcctg tggtcttagc acaaaaaagt aatataacgc tatacccacg accacaacca
    21481 caatcaatgt tagtagaaca actaataatc cactcttttt tctgccacca gccatgatta
    21541 gcctcctttg aagggcatcg ggtaatttcc taaaactttg ctctaattat acggcaaaaa
    21601 taaaaaatta atagtccaca agggcaggga aaattttgta tttaatgatg accagtttgc
    21661 ctatgaaaga ggccctgcac caggcagggc ctctctgacg tgcctttcag attggctcca
    21721 taaatgaagc actagagaga ttgaacaatg ttcgagaaga tcttaccgat gatcgggccg
    21781 agcagggcga gaataacaat tacaacaaca gctaccaaaa caagaatcaa agcatattca
    21841 accaggccct ggcccttttc cttaggagcg aacaacatgt ttttcacctc cttcctgttc
    21901 aaaactcagc ttgattaaag cttgttaaca ttatattcaa aacacgctca aacgcaagct
    21961 ttagacgaaa gagaccccgc gccatgcagg gcctcttcag cttgcttctc taagctggct
    22021 tcgtacgaag cacactagat ggactggacg atgttggaga agatcttgcc gatgatcggg
    22081 ccgagcaggg cgagaataac aatcacgaca acggcgacca acacaagaat caacgcatat
    22141 tcaacaagac cctgaccctt ttccttggga gcgaacaaca tggatattca cctccttccc
    22201 gttcaatgct tagtctatga ttagacttgc ttatattata ttcgaaataa attatgatgc
    22261 aagacaattc cccttacaac ttccttacaa cccgatacaa gttgtcgcaa cctgagagct
    22321 gcctctgcgt atattgtcat tgaatagaat taagctataa ttaacttgat ttctctgaac
    22381 ccatccaccc cgtgcgggtg gccagccaca aaggaggcat gatgagcgat ttgtttgaac
    22441 gggtaaccgg cgatcaggat ttcctgacca agctgatttc taaaatcccc ggctttagtg
    22501 gatacgtgga gcggcaaacc cgccgcgcat ccgataaact gctgcgcgag accattgcca
    22561 accgctacga agctctctgg cagcgtattt cagctttgca ggttgctctg attaacgaag
    22621 gtggcatcct ctatgtggat gacctcgaat cagccgccct caagctccgc cagttcattg
    22681 accgcgtccg cacagcctcc tatggctatg ccggtttctt tgatgccgtc aaagtcaacc
    22741 aggaagtgct cgccaaagtc tatgaatatg acctggcttt gctgaactcg gaagatgaaa
    22801 tggcccgggc cattgacaac gtcgaagcct cccttggcgc cgatggactg cccgcggcca
    22861 tccgcaacct gaccaccctt gcccagcaat gtgtggatgc cttcaacaag cgcgccgaag
    22921 tcatgctcgg cggcgttgaa taatcttttt cagccctcta gcatttaccc cagggagtag
    22981 caaaatggca agaatctttg atgttgttga atatccaagc gagatgatgg atgaaatcgt
    23041 ccatcgtttt cccgaacagg gcgtagccga cctgcgcatc ggttcccagg tcatcgtccg
    23101 tgaatctcag aaagctgtct tcttccgcga tggtcatgcg ctcgatattt ttgggccggg
    23161 ccgtcacacc atcgtcaccg ccaacatccc cttgctggtg gacctggttg gcaaagcctt
    23221 caatgatcgc acccccttca cggccgaggt ttactacgtc tccatgcgtg agtttgccga
    23281 ccgcaaatgg ggcaccccgc agcccatcct cgtccgcaat ccaggcatgg gcctgggcgt
    23341 agcgctgttg caggcttttg gctcctacag cttccaggtc aacgacccgc agcagtttgt
    23401 cacccagctt gtcggcgctc agggcatgta ccgcaccagc gatattgaaa accgcctgcg
    23461 caatatgctc ctctccaagc tgcaagatat cctcggcgaa accggcgcca agcatagcgt
    23521 tgccgagatg atcggcctga ccgaagaaat cggcgctggc gtgcgcgcca aagcccagga
    23581 tgacttcaag gccattggcc tcactctcaa gtcttactac atcgccaacc tcaaaccgtc
    23641 cgagaaatcc gccgaagaac tgcgcgccat gggtatgctg gatatgcaga catacaccca
    23701 gctccaggca gccgatgcgc tgcgcgatgc tgcccagaat cagagcggcg gcgccggcct
    23761 cacggctggc attggcgctg gcattgggct tggcggcgtc atcaaccaat ccctccaggg
    23821 gatgaccggc gcccaggctc agcctccggc tggcggtgca gcccctgccg gtagtggtac
    23881 tgcggcagct atccccgccg tcatgacccc ctctgaggcg gcccagattc tgcgcgtttc
    23941 cgaagaagat gtcgtcgcgg ccatcaccgc cggcgacctc aaagccaaga agattggcaa
    24001 cgcctaccgc atcagcaaag aagccctgga agaattcctc aagggttaag ccctttggag
    24061 cattcagagg gcccggcaag tttcgccggg ccctttttat tttttagcgc cggttcggat
    24121 ggttgggatg aaacttcacg aataccaatc caagcatatt tttagccgct acgatatccc
    24181 cattccccgc ggcagcgtgg ccgatacggc tgaagaagcc cggcatatcg ccgaagaatt
    24241 gggcggtcgc gtgattgtca aagcccaggt cctggtgggg gggcgtggca aagcgggcgg
    24301 catccgcctg gcaaaatcgc cagatgaagc cggtgacctg gctcgccaga tccttgccat
    24361 gaaaattaaa gacctgcccg tacgccgggt attgctggac gaagctgtca atatcaaaaa
    24421 agaaatttac ctcggcatca ccaatgatcg ctccagccgc cgcccagtga tgatgatttc
    24481 ggctgccggg ggtgtagata ttgaagaggt cgcccggctc acccccgaaa aaattgtcaa
    24541 aacgcacatt gatccgctca tcggactgcg ggaataccag gcccgtgata tggccgttgc
    24601 catagacctg ccgcaagacc tctggcgctc cttcacccag atcgctgccg ggttgtggcg
    24661 cgcctacctc gaaaatgacg ccaccttggc cgaaatcaac cccctggtca tcaccggtga
    24721 tcaacatctg cttgccctgg atggcaaaat gatcctcgac gataacgccc tctaccgtca
    24781 taccgacctg gctgcgctgc gtgatgtgga cgaagaaacc tcggctgaat cggaggcccg
    24841 caagaacggc ctgtcttaca ttcagttgga tggtcaaatt ggctgcatgg tcaatggggc
    24901 gggtttggct atggccacta tggatattat caagctttac ggtggtcagc cagccaactt
    24961 cctggatatc ggcggcggcg ccagcgccga aaaagtagcc gccgcgtttc agatcattct
    25021 ctcagatgtc aacgtcaaag ccatcctgat caacatcttt ggcggcatca cgcgctgtga
    25081 tgaagtggcc cgtggcatcc tctccgccat tcgcaaagcc cgcccacgta cccccatggt
    25141 ggtgcgcctg gtcggcacca acgccgttga aggtcgccgg attcttgccg aagccaacat
    25201 gattaccgcc gaaaccctgg ttgaggcagc tcaaaaagcg gtggccctgg tcggagaggt
    25261 gcgcacatga gcattctggt gaacaaagaa acccgtttgc tggttcaggg catcaccggg
    25321 cgtgaaggtc ttttccatac tgagcaaatg tatctctatg gcaccaacat tgttgccggc
    25381 gtcacacccg gcaagggcgg cgaatgggta ttgggtggca aggtgccagt ctttgattcg
    25441 gtccggcttg ctgtagaagt taccgaagcc aataccagtg ttatttttgt cccggcgcgg
    25501 tttgccgccg atgccatcta cgaagccgcc gattcgggta tttcgttggt cgtctgtatc
    25561 accgaaggcg tgcctgttat ggacatgctc cgcgtccgcc gctatctgga ccagcgtcat
    25621 gtccgcttga ttggtccaaa ctgcccgggc attctcacgc ctggcgaaag caaagtcggc
    25681 atcatccccg gtgaaattgc ccgccctggt ggggtgggtg tggtttctcg ctcaggcacc
    25741 ctcacctatg aagtcctctt tgccctgcgt caggctggca tgggcgccag cacttgcgtg
    25801 ggcattggcg gtgatcccat caacggcacc agttttacgg acatcctcga gctttttgag
    25861 gcggacgccc acaccgacca ggtggtcttg attggcgaaa tcggcggctc cgatgaagag
    25921 cgcgcagccg acttcattgc cagccggatg accaaacccg ttgcggcgtt tatcgccggg
    25981 caaacggctc cgcccggaaa acgcatgggg cacgccggcg ccatcattga acgcggcagc
    26041 ggcagtgctg aagaaaaaat ccgcaccctg caggcatctg gggttcgagt tgcccgccac
    26101 cctgaagaaa tccccaccct gttcaaccat taaatatcaa aagcgctccg aaaccacaaa
    26161 tttcggagcg ctcttgattt agctcaaatt atcccttgat tttcagttcg ctgcggtgcg
    26221 cttccaggta ggtcaaaaca tcagcaaccg accggatggc acgcgcatct tcatcgccaa
    26281 tattaatgcc aaacttttcg cagaacccgt caatcaggaa gaactgatcc atcgaatcag
    26341 ccttcaaatc ctcgataaag cgggtctcgc gtgtgatttc actgccgtca acttttaaca
    26401 cgtcaacaat cacagctttt accagctcaa aaatatcatc catctttgcc tccaggggaa
    26461 aatccattca actaagtgaa ttgtaactag cctcaaaaat ctgtcaaggg ccaatccttc
    26521 actaaaaagt taacccgctg gctgccaaat agttgcgccc atgaatgctc agaggctttg
    26581 acgcgctttg ttccttccca agcgcatccc agactggtat actctttaac aatacggttg
    26641 tctttgagtg aatttatgga tttacccgga atttcctccc gcaaagcgga tcatatccgc
    26701 attaacctgg aagaagatgt tgcttccggc ctgaccactg gcctggaaga ttaccatctg
    26761 gtccaccagg ctcttccaga aatcaacctc tccgctgtag atttatccca aacgctgttt
    26821 ggccgccgcg tgcagtcccc gattctgatc tcttccatga ccggtggtac aacccgggcg
    26881 gcttcgatta atcaactcct ggcgcagacc gcccagcaga ccggtgttgc cctcggtctc
    26941 gggtcgcagc gcgccgccct ggagcatccc gacctggcgc ccagttttca tgtccgccgt
    27001 ttcgcccccg atattttgct gctggcgaac ctcggcgccg ttcaattaaa ttacggctac
    27061 agcgttgatc actgccgccg tgcggttgag atggctgaag ccgatgccct catccttcac
    27121 ctcaacccct tgcaagaagc ccttcagccg gaaggtgaaa ccgattttgc cggtctgctc
    27181 aataaaattg agcaagtgtg ttccaccctc ccggtgccgg tggttatcaa agaagtcggt
    27241 tggggcattt cagagcaggc agcccggcag ttagccaatg ccggcgtcgc tgccattgat
    27301 gtcgctggcg ctggcggcgc ttcctggtct caggtggaaa tgtaccgcct gacagacgaa
    27361 acccgcgccc gtgttgccgc cgcttttcgc agttggggca tccccaccgc tgaatcgatc
    27421 cgggctgttc gccgcgtagc gccaggtgtc aaagttttcg cctccggcgg cttgcgtgat
    27481 ggcattgacc tgaccaaatc cctcgccctg ggggcctctt tgggcgggtt ggcaagtcct
    27541 ttcctcaagg ccgccgtcca atccgaagaa gccgtgcttc aactcatcga agttttgcag
    27601 ggccagatta agatcgccat gtttgcctgc ggtgcgctga acctgacggc gctcaacgaa
    27661 tcatgtcttg ttccggtgaa atcagcggag taatcatgcc cttatcagat tgggttaccc
    27721 aaatgcgtcc cgcagtggaa gaaacgctgc gccaaattgt ggatgcctcc ttcccgcccg
    27781 agcacgacag tcaatcccct ctctgggctg agctgcgcca catgctcact tatcacctcg
    27841 gctgggatgg ccccggcagc ggccccgacg cccagggtaa gcgcattcgt cctctgctgg
    27901 tcttgctctc tgccgccgct gttgacggca gctggcagtc ggctttgccc ggcgcggctg
    27961 cggttgaact gctgcataat ttctcactcc tgcatgatga tattcaggac caatccgaaa
    28021 cccgccgcgg tcgcccaacc gtctggacgc gctggggcat cccgcaggcc atcaatgccg
    28081 gcgatgccat gttcaccctg gttcccctgg caatcaacca actgtcatca caccatgcgc
    28141 cagaaacggt tgtggctgcc aatcgcattg tatttgaagc ctctgcccgc ctgaccattg
    28201 gtcagtatct ggatatttcc tacgaacagg ctcgcagcct gccgcttgaa gcctattggc
    28261 cgatgattga gggcaaaacg gctgccttgc tggcggcctg tgccgaaatg ggcgccctgc
    28321 ttggcggcgc caatgccgag cgccagcgca gttttcacgc ctttggcacc tcgctggggc
    28381 tggcctttca ggccttggat gatatccttg gcatctgggg ggatgaaacc cttaccggca
    28441 aatcggccac cagtgacctg gtgaccggga aaaagaccct gcccgtgctt tatggccttc
    28501 aacagaatgg cgaatttgcc cgccgctggc tggcaggttc catcgcggct gccgatgcgc
    28561 cagagcttgc cgccctgctg gaagcggagg gcgcccgcga atatacccag tctcgcgccg
    28621 accgcctgac cactgaggct ctctcggcgc tcaaccaggc cagctcaccc ggcgatcccg
    28681 ctgattcgct ccaccaattg accttatcct tgctgcatcg tcagcaataa cttacaaacc
    28741 actggagata acctcatgga cgacaaaaaa caatctgttt atgatgctgc tgatttaatt
    28801 ctcgacaccg aaatggacct cgataccatt caatcctctg tacggcaaat tttgaccgcg
    28861 gttggcgaag atgccgaccg ccagggtttg cagcgtacgc cagagcgggt tgcccgtgct
    28921 taccaggaac tgctgtccgg ctatcgcacc gatccgctcg cattggtcaa tgatgccctc
    28981 ttcgatgtcg aatatgatga gatggtagtg gttcgcgata tcgaatttta cagcctctgc
    29041 gaacatcaca tgctgccctt catcggccgc gcccatgtgg cttacatccc caacggccgc
    29101 gtaattgggc tttcaaaaat tccccgcatt gtcgatatgt ttgcccgccg tctccaggtt
    29161 caggagcgca tgacccgcca gattgccgac ctgctctgcg aactgctcaa ccccgccggg
    29221 gtgggggtgg tggtcgaggc gctgcacctc tgctccatga ttcgcggcgt caagaagcac
    29281 gactctcgca tgaccacctc caccatgttt ggcgtctttc gcaccgacct ggccctgcgc
    29341 atggaattcc tcgacaatat ctcccgcagc gctgagccct tgcacttcta gccagaccgc
    29401 tcccagatta tgggcacaac ttaatttccg gatgggcaat tgcccccgag ccaggcagca
    29461 gccgtgcggc caggtctgcc agccgttccc ccgtttcagg gtgaatcaag tccggcagta
    29521 gttccgccat tgggatggca ataaagctgc gctgccagag agcctcatcc aaaacaaccc
    29581 catcaaaaag gataatatca atatccagag tccgcggggc gttcttgtcg gctgtccgca
    29641 cccgccccag ccgtgcttcc agcgggcgca ttaccgcctc tttgagcgtt ctggcatcaa
    29701 cctcggtttg catccctgcc gccaggttga gaaagttcgg cgcattggcc gagccaatgg
    29761 ccaccgtttc ccaggcggta gaggtgttct tcagttggca gcaggtacac agttctgcaa
    29821 cggcccggcg caaattttcc accgggttaa tattggaacc gaacagcaga taagcatcat
    29881 gcatacgtca ggtggccggg ttaaaccgct caatttctac ccctaccgaa cgggcaaacc
    29941 gcactgctcc gggcttttcc acccgcaccc acacgccttt gacccccggc tcttccagac
    30001 ataaccgggc aatgtcttcc gccagcgcct caaccgtata tcgtcctact ttttcagcat
    30061 gggcaaataa tttcttggca accgtccggt aattgacgca ttgattaatg tcatcgctct
    30121 gcgccccggc gtgaatgtcc gtgtacatcc gcacattaat caaaatatcc tgcggctggg
    30181 cgcgttcatt gtctgagatg ccaatcaccc cgcgtaccag cagatcctga atcagtaccg
    30241 tatccatcat ccaatcctcc agtaaaaatt gccagatacc cctttatacc acatgccgcc
    30301 cgccatccag atgcacaatt tctcccgtaa tataagccgg gccgctcaac aggaagatca
    30361 gggcttcttc cagttcttct ggttttgccc agcgctgcat gggcacatct tgcaaaaccc
    30421 catctgtgga tgccccgtcc gccggtggca gcatggcccc cagcgccagc ccgttcacgg
    30481 caatccgcgg tgccagcgcc tgggccaatg cctgcgtcag cgccgccagt gccgccttgc
    30541 tgatggtata aggcaggtga tccttgcccg ggcgcagcgc ccgccagtct aaaatattga
    30601 caatccgtcc ctgcccattc accggcaggc gctttgcaaa tgcctgactc aacaaaaacg
    30661 gcgtggtcag gttgatgtcc aggtgcttct gccagtcggc gagccccgtg tccgcccatc
    30721 caagcggctc aaaaatggcc gcattattta ccagcgcatc cagtggcgca cagtcccagg
    30781 cggcttcaat cagtgcaggt gccgtctccg gtttgctgaa atcgcactgg atgacccagg
    30841 cgcgccgccc cagcgcttca acctcgctgg cggtttgccg ggccgcctct ggcgacgcag
    30901 cgtgatggat caccacatct gcaccgcccc gtgcggcagc cagagccagc agccggccca
    30961 cgcgtcgggc tgcgccggtg atcaagatgg ttttattctc caggagaagc tgtgttttct
    31021 tcaaccgatt gcctccttaa ccaccccagt agccacaggc tgagcgccag tcctagccat
    31081 gccacaacct gccacaaccg aatgctgccc gttaacatca cgctgtcgcc gcgccaggtt
    31141 tccaggaaca gccgccaccc tgcgctccat gccattaatc tcagaaactg ctgtccggct
    31201 ggctgtttcc cgcctctggg ccacaccagc gccagcgtga ttgcccctgc cagcatctca
    31261 tacacctggc tggggtgccg catctcattc cacaggcgaa tcccccaggg cagggtggtc
    31321 ggcgcgccgt acccactccc ggaggccaga tttgccagtg gaatcacaat cgccattaac
    31381 gccaaccccg gcgtcagcac atccagcgtg ggccacaggg gcagcttttt acgctgccca
    31441 tagatcaacg ccgccagtat gccgaacaac agcccggctt cctcatccag catttgtccg
    31501 cttggtaaaa tcaaagccag cggctgggcg gcaaaagccg ccgggttgcg cgccgcataa
    31561 gccagcctgg ctccaatcaa tccggcaatc aatcccgtaa ttgccaggtt gaatatcacc
    31621 tctccctgaa tatccgtccg cctggcgcgt ttcaataact ggtcgcaaag cgtcaatgcc
    31681 agccacaggc ccaggatcaa aatcaccccg cggctttgaa tggatagcgg cccaatctcc
    31741 aggtagggga acatcttatt cttcccctcc aagcagggca tccagttcac tgttgagcag
    31801 cgccgcgctg atcgcgccag tatagcgccc tcgcacaatc ccatctgagt ctatcaaaat
    31861 agacgtgggg agcagcctca cttgataagc ctcactgacc acgctttccg ggtccagcag
    31921 cagcggaaac gtcaggcctt tttcaacaaa aaaagcctcc gccgcttcca ctgtatcgcc
    31981 ggctgtcgcg ttcacggcta atacaacaaa gccctcatcc cgccgctcag catagacaga
    32041 ttccaaatcg ggcatttccg ccttgcaggg cgggcaccag cttgcccaga cattgatcaa
    32101 gaccacctgc ccgcgtaaat cgctcagccg aatttgctcg ccggtcgatg tggtcagcat
    32161 aaaatctggc gcccgaaagc ccacttgcgg cgctgcgacc gcttgcgcag gcggcttaaa
    32221 ccagatggcc gttgcagcca tccagagcgc tcccagcgcc agcagcccca gtgatatcaa
    32281 tgcccggcgg cccggcttgt ccatattatg gcgcggcgtt cacaaactca gcccaaatct
    32341 cattgtaaag agcttctgct tccgggctga tgggcattaa aagctcagca ttggtcaatg
    32401 attctgctgg tggaaacaca atcgggttgt tgcgcaagtc tgggtcaagc agcgaaagcg
    32461 ctgcttcgtt gggcgttgca tagtaattgt ggttggcaat ttctgccgca atttctggac
    32521 gcaggataaa attcaaaaac aattctgccc ccaccgggtt tggactgctt gccggcacca
    32581 caaagctgtc gctccataac accacgccgt cttccggcag gatgtattga atatccggat
    32641 tgacagcttc cccttcaagc gcgtcatagg catagcccat ggccacgtcc agttgatact
    32701 cattgattaa ataaacgccg gttacgccgg aagacatatt ctcaatgaat tggacattgg
    32761 gccgcagttc catcaatcgt tgtcgagcgg cttccagttc tgccgggttt tctgagttgg
    32821 ccgaatatcc cagggatttc agtgtaaatc caattgtttc acgcccttca tacagccaca
    32881 atcccacccg cccctgaagt tccgggttcc acaaatccgc ccaggttcga atgggcgggt
    32941 caatccgctt agtatgatag ataatgccgg ttgtgcccca gttaaatggc acagagtagg
    33001 tattggtcgg atcaaaagcg agtcctcgga agttgggcga gatatttttg atgtttggga
    33061 ttgcagtcgg gtcaattggg tgcagcatac ccgcgcggat caatgcgtca atattatcgt
    33121 tatccaatac caccacatcc aattgatcgc caattttaat tcgctcggcc gcttcctctg
    33181 tcgcatcata ggtcaggtat ttcaccgtga ccccgtattc cgcctcaaac tgatccaata
    33241 cagattgcgg catatcttcg gcccagtcta gaaagatcag ctcggggccc ggtgtaggtc
    33301 cggcgggcag ggttggttca ggagtgcacg cgctgaatat tatggtcaat agtagcgccg
    33361 gaatgatgac aaatagcctt ttcatcttat tcacc
//
LOCUS       DAOPVS010000015        32558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1291_length_32558_cov_59.336830, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000015 DAOPVS010000000
VERSION     DAOPVS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 32558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32558
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1291_length_32558_cov_59.336830"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKL11_02910"
     CDS             <1..576
                     /locus_tag="PKL11_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12580.1"
                     /translation="KSTLILHLNGILSGEGVLRICGQAVTAKNLGEVRARVGLVFQSP
                     DDQLFSPTVFEDVAYGPIYQGLTPGEVQARVEQALEAVHMEAYIQRNSYHLSIGEKKR
                     IAIATVLSMQPEILVLDEPSAGLDPRARRNLIELLAELPQTMLIATHDLALVRDLLPR
                     TLLLDQGQVTADGPTDAILSDAELMAAHGLA"
     gene            752..1450
                     /locus_tag="PKL11_02915"
     CDS             752..1450
                     /locus_tag="PKL11_02915"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNZ12581.1"
                     /translation="MSRSKLFKAIFALVLLVTTAACSLTNTTVPLPPTLSAEDMQATV
                     NAAATISIQTIAANLTSTALALPTDTPEPSATPTLRPSSTVAPTSTPPAATVTPIITR
                     APTRTHTPVQSPTNTQQPTSEGYNCTIMAVSPAADTVIGAGNDFDVRWTLKNSGSQTW
                     DNADVDYRWYSGDKLHTGNDIYDMAADVAPNATVDIVIDVKAPTTVGTYGATWRLMRS
                     DKVICTFDYRFKVK"
     gene            1564..2085
                     /locus_tag="PKL11_02920"
     CDS             1564..2085
                     /locus_tag="PKL11_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ12582.1"
                     /translation="MNTPKKPAEIADDVVVMLKYVLTVDGEEVDSADEKDPIAFLQGS
                     GEIIPGLEKALYGMKIGQSKKVTVAPAEGYGDHEPEAMMSVPRSEFPEDIPLEVGIEL
                     DVRDEDDEVMSATIVDVTKDEIKLDFNHPLAGKTLHFDITVVDLREATEEELEHGHVH
                     GDDDDEYEDEEEE"
     gene            2194..5799
                     /locus_tag="PKL11_02925"
     CDS             2194..5799
                     /locus_tag="PKL11_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ12583.1"
                     /translation="MDEIRQLEEAIAALEAQRPVLGDAVVELALDSMLQKLATLKAGQ
                     GEAEHEAQQRKMVTVLFADLSGFTALAEQMDAEDITDLMNALWERLDRIITQRRGRID
                     KHIGDAIMAVWGAEAAQEDDAEQAIRAALDMQAELMRLNNNNEIPGLKALPDEQTIAM
                     RIGINTGAALLGVVGTTGEITAIGDTVNTASRLQNAAPLNGIIISHDTFRHVRGLFRV
                     RALEPLALKGKAEPVAVYLVEKARPHAFRLATRGFEDLDTRMVGRGEELEQLQADYME
                     GLNEQNPRMVTIIGEAGVGKSRLLYEFSSWLELQNHLTAAFKIRATQQTSSVPGLLIR
                     QLLTTQLEIRESDPAEVIIARLAHGLEKTPGSEVPLNNRALILGTWAGFDLPDDNGFI
                     GLIGARQLRDRGMIHLAELLSASAAEVPLALLLEDLQWADEQSLDAIEFIQRACAHVP
                     LFTICVARPGFLERRPDWPALCSTMLELRPLNENESRTLIQEILQRARQIPEALCSLI
                     IQRSEGNPFYIEELIKMLIEEGVIQIREQEWLIDASRLETLPIPATLTGVLQARLDSL
                     PASEKLTLQRASILGRIFWDRAVAQLGKTEDRPEQDEAVQKPLNGLRNRRLIYRRDKP
                     AFEGTNEFIFVHVLLRDVAYESVLKRERRHYHAEAARWLGQTARAAGRADEYAVLIAE
                     HYEQAGEMLSAQEWYERAAGSASLRYANAEARRCLTHALALTPANDPHHSYDLRLARE
                     KVLNLLGDRGAQLEDLNALEEIANALGDPRRQAEVALRRAHYAEITGDYGEAAAHASR
                     AIRLAQAGGDARSETAGHLQHGQTLRRQGLYEAAGEEFEIALKQAEALGVTAIEAECL
                     HSLGKLSFHQGNYAAASDYYARTRVIHEQNRDLQGVGAVLNSLGIVADSQGDYPTATQ
                     YYTEALGVFRKIGDLWGQGLAYNNLGIVSGEVGNLSDARDYYSQALQICIQTNDRYGE
                     GMAYNNLGIIADALGDYGGAQRYYEHGLKIMRETGGRQGEGVVLASYGLLMVHAGDAN
                     GALTLSEQALEIADELGEQRVAGYALTNQGHALLLLGKNAEALAAYHRAAMIRQELGD
                     LTVAIESQAGEVRCLGQLGRSQEALTLTENILKHLETNTLDGVEEPGRVYLTCYDVLH
                     AHQDERAASVLKTAQENLIGKANRISDVESRRGFLENVAVHRQIMTLFTGQGDAA"
     gene            5796..6335
                     /locus_tag="PKL11_02930"
     CDS             5796..6335
                     /locus_tag="PKL11_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNZ12584.1"
                     /translation="MKLINIPPQKLISGAYALWEDQWLLLTSGDFEEKRFNAMTVSWG
                     SLGVMWNRPFAQVVVRPTRYTFEFMEKYETFTLCAFPLQYRPALQLLGARSGRDSNKM
                     TECGLTAVAASQVGAPVYAEAELVIECQKIYWQDFDPDHFLDPKIGNNYAGKDYHRVY
                     YGQILAVSGIEQYLAAEAK"
     gene            6355..7482
                     /locus_tag="PKL11_02935"
     CDS             6355..7482
                     /locus_tag="PKL11_02935"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ12585.1"
                     /translation="MPLFGPPNVEKLKAGGDVLGLIKALGYEGDRGDTRRAAIAALGQ
                     LGAAAVPALSEALQNSSLPVATAAAEALGRTHAQQAVKPLIQLLRQPNRFLQMAAVQG
                     LKLLPTAESMAALQEALLGNEETARAAAEALAQMNLPAAWQSLAEMLGHSEFNARMAA
                     AHGLVEVGTAGTPLLLDALENGNENARRSAAELLGQIRPMEALAVLLEMMADSSDYVA
                     LAAGDAVWRYGDAAIIPLLKVLIQGNDRLKTAAAQQLIRLGDRAERPLLAALQNADPD
                     TRARVAVILGKIGSGWTVQPLIALARQTDPSLRYAAVQALAEIGGAGVVEAVITALED
                     ENPYIRRAAREALIRIEETAETRALRNQIQGALQSHPPDRE"
     gene            7487..10603
                     /locus_tag="PKL11_02940"
     CDS             7487..10603
                     /locus_tag="PKL11_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ12586.1"
                     /translation="MSKSIPEQIQDLRNAIAAQEMLRPTLGDAIVDATIVVLNQKLTA
                     LESELGKSEEQRKLVTILFSDIKGSTAMAEKMDPEEWSDIMKQAFQFLIDPIYRHQGL
                     VARLMGDAILAFFGAETVREDDAERAVRAGLEILTGMKPFQEAIAQQHGTEFGVRVGI
                     NTGLALLSNVGTDQAGEFTALGDAVNLAARMEQSAPPNHLLIAPDTYALVRERFEIEA
                     QAPIMVKGKSEPIPVYVVVRELPKSTVEVRRGMAGLDVPMVGREAEMERLRAGYQQAR
                     EGKANCILVTGEAGSGKSRLRYEFRRWVERQGEVVQMMQGRADAATWHAPYSLLHDLF
                     GNACGIQESDPAAEVRCKLETGLSPYLEAERADLVGQLIGFNLADSPAVRAFGQSTAW
                     APLAEVYLAQYFQQASRVRPMLLLLEDLHWADQASLDFLLGLPKQMPTGALMLLGLGR
                     TELLERLSEDCCEKINLGQLDSTDSQHLLETLIPQADRLPEAFKAMVIGHANGNPYYL
                     QEIVQMLLDEGLLSLEGGAVMSGQADWDNLHLPPTLQGVLQARLDSLSAVEKQFLQRA
                     AVLGEQFWDEALTQLHTEQDAIADIEQPIHNLEKRQLIYPVEVSAFDGLAEYIFKHAL
                     LRQAAYDTLLLKLRRVYHRQAAQWLLERCGDRMPEYLGKIGDHFERAGENEQAALFYQ
                     QQARDAYARSAFRDALPTVERAIRLTRPENEPQLAQLHLQMSNNLEKLGEYDAAIEHL
                     NTCLGLARGTGSTGVEAEALCIMAWIMGIRGQDAEVEPYSRKALELAEQAGDMRIMVR
                     ALTQVAGLYEAGEPTKALEIYEQALVIARESQNAAQIATTLLNIGNTELNLRHLEAAE
                     TYYLESQAIYEKSGNRWGLENCINNLAIIYKLRHAFEKGRENSQRALQIAEEIGDQEG
                     QALCLISLAHCAGGLQRIKEAQEHLRAAVEIIQKIGLDLNLDLLNIIGRVLVAQGRYQ
                     EAAEYAGLVLTEPDLIFEISQDVHDLLDEIKMHLPETTIEAAKLRGKTLDHENAIRRF
                     LEESRQTE"
     gene            10624..12885
                     /locus_tag="PKL11_02945"
     CDS             10624..12885
                     /locus_tag="PKL11_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNZ12587.1"
                     /translation="MPALDPVLIFAIATSIIYILLLGAVFRRTIGGDRSLLILTVVLI
                     LGLSWSLSLTFPRLALFSGIRIETLKYRIPFYHIITLTLLFYALTRSFTRRQIPWWFW
                     ALGLAWLVLTVIIYENVLGTPEEWWLGGNRTLPRSMGNTLIIGPVWAFLVISGYVILV
                     REYKQLTSPLHKNRFRYWMLGQTLMAIGGGLALFNQRIVGGTAFLLANVAVTYVLTTY
                     RPFDLREGAGRFSVFLINTVLAVGIYSVGFWAASWLVHLYYTTNAWLIWAGMGVVLTL
                     LVSPLLMRTHRWLTDMIFGSGYDARDAVREYSASINNILSLEQLTNVITGFMQDTFQV
                     KHSQLVLVNFEEVKASDPPQGYYLLKVVASGEGLPTVAIMADDSPVGEYLRNARIPLT
                     QYEIDVLPRFRETAGPERTWLAGLSADVYVPIFSKGQWVGLLALGAKTSGERFYDNDL
                     ALLSTIADQTAVALENARLFDDLRQRNEENEHLNLELTEAYQELERLDQAKSNFLNIA
                     SHELRTPLTQVRGYTDMLNESISEKNITPETAGIMAQGLRKATLRLESIVDIMFDVSR
                     VETGTLELDLVPLGMASVLQLATDAWQDALRRRKISVNIVGVDNLPTIQGDLQRLKQA
                     FSNVIQNAIKYTPNGGHIQISGYLLTNPENPEEQAVEIIIADTGIGIEPAELERIFDA
                     FYGAGDPNLHSTGDFKFKGGGPGLGLTITRGIIDAHGGRVWAESPGYDETHYPGSKFH
                     ILIPVNPPTPPLA"
     assembly_gap    12929..13028
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13029..14516
                     /locus_tag="PKL11_02950"
     CDS             <13029..14516
                     /locus_tag="PKL11_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNZ12588.1"
                     /translation="TQKKRRQDDYTPGGENNRRPDRAVIAYANLIRRYFKGTRPIVLG
                     GIEASLRRVAHYDYWDDRIRRAILFDAKADYLLYGMAESGVVALAEALREGRSPLDLR
                     GLCYLSKTPPAEAIELPSYEEVTADKLAFIRMFHTFYQNNDALTARTLCQRHGDRYLV
                     QNPPGMPLKQADLDAIFALPFERKVHPFDAQGGPVRALETIRFSISTHRGCYGECNFC
                     AIAVHEGRTVQWRSQGSIVDEATRITHLPGFKGYLTDVGGPTANMYGYECDKKLGMGS
                     CANRRCLTPEICRQMRPDHRKQMQLLEELRQIPGMKKVFVASGIRYDLVLADRMHGRA
                     YLRAVVNHHVSGQMKVAPEHSEDRVLRQMGKPGNDSLLRFKAMFETLTAEAGLPQFLT
                     YYLIAAHPGCTEADMRRMKDFVSSKLHITPEQVQVFTPTPSTYASLMYYTELDPFTLQ
                     PIFVEKNLHAKEVQKEIITGKGPQPPQRSQPLQRDKPLPPKKKRS"
     gene            14513..15304
                     /locus_tag="PKL11_02955"
     CDS             14513..15304
                     /locus_tag="PKL11_02955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12589.1"
                     /translation="MKIENRRIILTGAASGIGRALLEALCQIPCHILAADRNGPALAD
                     SLAALEGCPAQLSGLEVDLSNSAGVDALFDEAQARMNGVDICIANAGFAYYEKITQPD
                     WAHIETIYRLNVFSAIYSLEKLQLLAAGHDFRMVMIASGMSHLAVPGYAIYGSTKAAL
                     DRFAEGLRYELARPEQLMLVYPIATRTNFFQQAAHKTPVPFPSQTPEVVATAILRGIR
                     RDRRRVYPSAIFSFILLLDRFLPLTRRLYQAHENKVFKQWQQGQN"
     gene            15372..17627
                     /locus_tag="PKL11_02960"
     CDS             15372..17627
                     /locus_tag="PKL11_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ12590.1"
                     /translation="MNFIEDLLARLPDWFQDEEQFKLAHLTHQLYPYTALFTPIQINQ
                     LTVKNRIVMGPMGNISVADEHGRPSSKMIQYFAERARGGVGLITSGLVPFSQGIDPSV
                     TEADGRSYFPRIEGSRTVYAGWRDLAESIHAYGSRFFIQLTPGLGRVGSPEVLMHKYK
                     LPVSASWNPNFYIPAVPCRPLTDAECRKIIQRGGQAAADAKAMQIDGVYLHGHEGYLL
                     EQMTNRAFNRRKLGRYADWQAFGIELVETMRRRVGPHYPIMYRIDLSLALNATYQGKM
                     QTEPTLRHFREERSVEETLAYMVNLVRAGVDCFDVDLGCYDNWWLPHPPNSMPSGCYL
                     AVAQLVKNTFTAQHIQSNAGLPVPVVAVGKLGYPDLAEKALRDGLCDMVMLARPLLAD
                     PDWANKAFAGQVQSIRPCIGDQEACINEFIEGGHSQCAVNPRTGFEDIIDRDPCPAQQ
                     IKRVAVVGAGPAGVICATTAAARGHQVTLYEKRSQIGGMLVPGSVPKIKYEVANYLTF
                     LKNEIQDSCERHNLALKLNTEVSLAMLQDGKFDAIVICTGGTPAMPPIPGVDHPDVIQ
                     AVDLFLNPQLAESAEKVVIIGGGAVGCEAAHYLAGEYNKQVTIIEMLPVLMKGVCTAN
                     RGHLVHEMEKLGVKIWIHTRVKAIDAGRVTVERNISPTTPDPYNTWTPLLPDNIPNPL
                     ARPIREVWQTEFLDADLVVLAAGLRRNTALYENCIQANAAPEIHTIGDAFQIGRVFEA
                     VKAGFAIGRSL"
     gene            complement(17738..19045)
                     /locus_tag="PKL11_02965"
     CDS             complement(17738..19045)
                     /locus_tag="PKL11_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNZ12591.1"
                     /translation="MKKNPKPALIKDQKFTLIAMSVLIVILAGVLIGVLIFMKNQPIE
                     TRGVPTIVAVDPLEPDSSKWGINYPNQYTTLLQTEFNNTRTTYGGSEPFSKLEEDPRL
                     VELFAGYPFSKEYNEERGHRNALTDVRATKRLSDKTPGTCYSCKSSNNPQLWSEMGME
                     AYDAMPFAQLGEQITEPIGCANCHEAGTMRLVVTNPALENALVAQGLDWQTFTRQEMR
                     TVVCANCHVEYYFEKGTNYLTFPWTNGTTIEDIEAYYVEDGHVDWTHPDSGADMIKMQ
                     HPDYELFSAGSTHYEAGVACADCHMPYTRDGAVKFSTHDVHSPLFNPASTCGTCHTDV
                     DYVVGRVEEIQGQVWQTMSASEDAIVAAINAISTAAASDGVDADKLAEARLLHRSAQL
                     RWDFIAAENSMGFHNPEEALRILATSIDLARQAQITAMEAMGK"
     gene            complement(19048..19557)
                     /gene="nrfH"
                     /locus_tag="PKL11_02970"
     CDS             complement(19048..19557)
                     /gene="nrfH"
                     /locus_tag="PKL11_02970"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNZ12592.1"
                     /translation="MKKYLPLLIAGIAAIAVLAVFAYSTEFITYLGNDPTTCNNCHVM
                     DGVYEGWFHAGHQQAATCNDCHTPHEFIPKYWIKAKSGFNHVTHFTLGNIPEPLRAKE
                     ETDKIIQENCIRCHSETVSMVSVGQEDTDRYCFDCHRSVAHGDRGISILPYQDKGIYA
                     PSVLSEEDK"
     gene            complement(19656..22445)
                     /locus_tag="PKL11_02975"
     CDS             complement(19656..22445)
                     /locus_tag="PKL11_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ12593.1"
                     /translation="MSRTTDFSSHPTPQPPEIPNTAPVITGASGLRSFLRAVRYLLLR
                     GLLIFSTIFVGVFITILIVNQPVQLGYGTMPPQLDMNLANHIEQYLRSYRVQTPGFVD
                     LSETEQDEIITSLRAELIDEVGLDLPYLPRHLLWTLKALKFDWGRLQVVSVRPLTMFN
                     RSQLSLDLNDILLQYLPNTLLLVVSSNLLIFLLGLPLALRLANKYGSWLDRALSVIAP
                     ISSIPSWVVGILLIAIFSAELKLLPPSGMLDTLPPAHWTGYILIVLKHMILPVLAIFI
                     SLFFQLVYTWRTFFVIFSEEDYVTLGKAVGFSPQHLQRKYILKPSLSYVITSFSLMLV
                     SFWQMTMALEVIFRWPGIGLLYIDVGLPNFWGESMYPGELLIAVSLVVMFAYLLGSVV
                     FLLDIVYVLIDPRIRLGQSDSVLRLRPLHRQGGFSWFPRRRTAPTHSLDRPPVPHPHP
                     RLNWADWKTNFHLAICNIIAWLKRAAREIHRYPTAIIGLSMISLMIIGSLYAVIALPH
                     EKIGREWGRTILTGQAVVPKQARPAWFNLFRSQDYLSTLVLDSAQGQAERVVRALPND
                     MWQITLTYTFDYTYADFPSEQFLYLESSYEEKKPYISMVWTTPDGREINLQGISVAPE
                     TSYAFEDYLHHRRIVSENPNWQNWFIFGDIFPTPAYYILFADPQADHPKLVRGQYTLR
                     IDGLTFEPDSDIESRLVMLGQVYGAAGTDYYRRDLLVPLLWGLPFALGFGLAGSLATT
                     LLSMLMAAAGVWFGGWVDGLIQRLTEANMVLPILAIAVLAYAFLGIDLWVVLTVVVLL
                     NVFGMPTKNFRAAFLQIKDATYIEAAQTYGASSWRIISKYLVPRIIPVMIPQMVVLIP
                     GFVFLEATLGLFNINSGYPTWGTVIYQALTHGALYNSRFWVLEPLALLLLTSLAFSML
                     GIALERILNPRLVED"
     gene            22587..24542
                     /locus_tag="PKL11_02980"
     CDS             22587..24542
                     /locus_tag="PKL11_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNZ12594.1"
                     /translation="MKRNPFYNRQRITDAASFIGRKSEIETLYSAVVTRQCRSLVGER
                     KMGKSSLLTHISNPNTMREYGLDPDRQLLLYFDLEGMASATVEDFWLEILEAIYDRMP
                     EGELAESVHKLISSGDVRFMAVRRLLRRIRDAGFDLVLCLDEFESLARNSRFEPDFYG
                     ELRSLAGELGLVYITASKRSLYELTYEHTDTLSSPFFNIFSELPLGSLDDDEANALLT
                     TLSASDGGPAFCAEEIAFARELAGGHPFFLQIAGFHLIETPGRGTARANEGYAQIRKR
                     YLAEAEDHYRYLWSQLSSTQQMALIHLEDAPEPVLRILRIKSLVEERNNKPVPFCQTF
                     AEFLQKQRTEQGTFQSTAAPTGGSDLIGRTLGNYRIVSPIGQGGMAEVFKGYQPTLDR
                     YVAVKVLSPRFAADPEFFERFQREAAAVARLHHPNIVQVFDFGNAESLTYMVMEYIPG
                     PTLKERIRTVRGLGQQMSHREVVAVTQQIASALDHAHANGLVHRDVKPANILLRATDF
                     EQATLTPSTSTQLVQNGYAVLTDFGVVKMLEGLKFTATGMTMGTPDYMSPEQARGEDV
                     TSSSDIYALAVVVYEMLVGQLPFYADTPLAVLLKHMNDMPPKPHTVLPELPIGVDLVL
                     GRALAKTPAERYATASDFSNALEVVLS"
     gene            24539..27859
                     /locus_tag="PKL11_02985"
     CDS             24539..27859
                     /locus_tag="PKL11_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12595.1"
                     /translation="MTRRSMISGVWVGLLLTLTGLYPAFYLFVGRVVIEIGNHLQRQF
                     GNFKIGVLDVPVSQIVLLVTTGICMMLLLLVGSIPTLRSGARGIKQGVKFGVIGSMVA
                     GLTVYFMLIAPTSAIVAGKDLFAHRLSVMAAQYPEHMVVNFARDVIVVAPGRMLITLA
                     LFALVGAVEGGLVGFLRRTQDPAKRPTALLDVPSTLRGHAAWFAESQEALVSGALTGL
                     ALGALMMLASIWAIANASGATAEEMWLMDVTQKALAGTPIGRMMDTLIANLLVPMVFV
                     LGTVAGGLAAFFPKNPPGRLGPRVVAAALAGAVSGMIFGIALSDQTRFALIISNQFMY
                     SNLISVQVDPSMPWELGEFMTVLATAPIRTLLVYLVPLPTTFGVTLAGTILGALQGLI
                     FSLIFMAVNLRPTDRAYKVRRELEQHPEVFLPRVYKLFQNSPMALAVLEHLYFGLRRE
                     PTKARLIGAFHTIATSPTRAADALEASATILSEQTGWKQHAEIAALHRILARGLKART
                     IAQLAAIQPMPEDQTTSLPQPLAKAGEFVSQIISEVKKIERVDDLSAKIIFLNNTLET
                     LRQGRIFLEATKKQDCCASTYPELIAFHAFLDQTSELALAGVRDIQGCADLISELKTR
                     TLTTAENQTLTVVLKNQGLNVAENIRLQVDDAPGLYTVVEGGQQTVAILTSQEPREFS
                     LTIRPLKNEPLRVSWRIVYDDALNKDRVLDFGDQIDFMDAQGSFQRIFPIPYVTGTPL
                     QTGEMFVGRDDIFEFVRENLFGTYQNNVIVLHGQRRTGKSSVLYHLNDVLKESHICVL
                     IDMQGKAARGEVDFLYSIADDIVYTLENHGITAPLPERSEFEDSPEFFFRSRFLRGVY
                     EALGEKNLLLMFDEFEELQKRVEDGKLSADIFPYLRNLMQHERRLDFIFAGTHKLEEL
                     ASEYWSILFNIAAYKRITFLDYDDVVQLVTKPVERYGLSYDPLALERIFQVASGHPYF
                     TQVICHELVAYHNDTERNYITTTCVDAVLDRIIERGEAHFKYIWAESSMEQRLLLVAL
                     AELLETMDNATLDDIAGVLEKRGRAVETTDLNEAAGKLEARDILLRSGPRSNLYRFRV
                     DLIRRWIYATRPAYEKIH"
     gene            complement(27918..28898)
                     /locus_tag="PKL11_02990"
     CDS             complement(27918..28898)
                     /locus_tag="PKL11_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNZ12596.1"
                     /translation="MKKLSLPIFVMLGLLFVLAACNFPVGNNQTDNSAAETAVVQTVS
                     AQLTQSAFSTALAQLTPVTQNTPTLTVVTAIPTPTLPVVAPTQPGIAPSPTAYTPPIA
                     TTGPTPTPAPLRPGGTFDAPFLTTPPTIDGVWDEWKNNAYPAKYVVFGGNNRTGEEDL
                     EGSFRLGWDNQNLYLAVKVLDEKYVQNAAGADMFKGDSIEILVDTNLMGDLNTTQLSA
                     DDYQLGISFGKPDVNGTREAYLWYPSNLAGTKSSVVIASVRGDGVTRLEASIPWSVLG
                     VSPSKGMTLGFAISFSDNDKESENVQQSMVSSAKNRTLIDPSTWGLLNLK"
     gene            29109..30389
                     /locus_tag="PKL11_02995"
     CDS             29109..30389
                     /locus_tag="PKL11_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNZ12597.1"
                     /translation="MKFESVCVLGLGYIGLPTASTFATNGLRVIGVDVNPQIIAGLQN
                     GELHIYEPGLRTVVQAALRSGNLRIRAEVEPADAFIIAVPTPFYEDKSADMRYVVSAT
                     EMILPHLRRGNLVVLESTSPPRTTLDLVRPILERSGLKAGQDFMLAYTPERVLPGQIL
                     RELIENTRIIGGIDRASAEAGRDLYGTFVRGDILLTDATTAEMVKLMENTTRDVNIAI
                     ANEFARLADRFGVDVWEAIGLANRHPRVKILNPGPGVGGHCISVDPWFFVDAAPDITP
                     LIKTARQVNDSQPHFVIDWLTRILGPLSGRKVAALGLAFKPDVDDLRESPAIEIAHLL
                     HQAGAQVACFDPFKPDAELPGLKTCQTIEAAIAEAEIVMMLVGHAQLRGLDPMALREQ
                     TTARIAVDMVNGWQPAAWQAAGFDLHRLGVGHRG"
     gene            30392..31522
                     /gene="wecB"
                     /locus_tag="PKL11_03000"
     CDS             30392..31522
                     /gene="wecB"
                     /locus_tag="PKL11_03000"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNZ12598.1"
                     /translation="MKNLRVLTIFGTRPEAVKMAPVVLNLARTPGIDAKVCVTAQHRQ
                     MLDQVLNIFNIQPDVDLNLMRPNQGLTELTADILTRLDPVLKDLHPDWVLIQGDTTTV
                     MVTALLCYYNRIKMGHVEAGLRTGDKWQPFPEEINRKIAGVAADLHFAPTEHSRQNLL
                     REGVPDEQIIVTGNPAIDALQMIVQRPAPPEVGQLFADHDIGPGKKGLVLITAHRREN
                     FGKPLENICTAIRLLAEQYADRLHFVWPVHLNPNVQEPAHRLLDQVSNVTLLPPADYL
                     PMVHLQRHATLVLTDSGGIQEEATGLGVPTLVMREVTERPEGVEAGVLKLVGTETERI
                     VGEAQRLLENPEAYQAMAHAANPFGDGHAAERITQALLKHHD"
     gene            31515..32540
                     /locus_tag="PKL11_03005"
     CDS             31515..32540
                     /locus_tag="PKL11_03005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ12599.1"
                     /translation="MTESICILPNTTGLGGPASFQARLIAGLHERHIPVHHNPDDPTC
                     RTVLVIGGTKRLDLLIKAKRRGLRIVQRLNGMNWIHRKKDTGIPHYLRSEWGNWLLST
                     IRRRLADEIIYQSQFARTWWQTVYGQVNARGRVIFNGVDLKAFTPEGKHNRPKDGLRI
                     LVVEGHLRGGYEMGLETALELCKALAPRLQQPLELMVAGDVDPDLKDWAQQRTAVPIR
                     WAGVVPRTEIPELDRSAHLLFSADLNAACPNSVIEALACGLPVAAYATGSLPELLADD
                     AGRVANYGTNYWELEAPVIENLADAAEVILKDEMHFRQAARTRAEAAFGLDAMVSQYI
                     EALLPGR"
BASE COUNT         7433 a         8020 c        10023 g         6982 t
ORIGIN      
        1 aaatccacct tgatattgca tctcaatggg attctgtctg gagaaggggt tttgcggatc
       61 tgcgggcagg ctgtgactgc caaaaacctc ggcgaggttc gagctcgagt tgggctggta
      121 ttccaatctc cggatgacca gcttttttca ccaacggtat ttgaggatgt ggcctatggg
      181 ccgatttatc aggggttgac gcccggcgaa gtgcaggcgc gggttgagca agctttggaa
      241 gcggtgcata tggaggccta tattcagcgc aattcttatc atttgagcat tggcgagaaa
      301 aagcgcattg caattgccac ggtgctctcg atgcagccgg agattctggt actggatgaa
      361 ccgtcagcag gattggaccc acgcgcccgg cgaaacctga ttgaattgct ggctgaactg
      421 ccacagacga tgttgattgc cacccatgac ctggcgctgg tgcgcgattt gctgccccgc
      481 accctgctgc tggatcaggg acaggtgaca gcggatgggc ctacggatgc gattctgtct
      541 gatgcagagt tgatggctgc gcacggactg gcataaggca tgattgctcg cggtggggaa
      601 ttgcactatt aagcagtccg aatggttggt taaattaagg taaactatcc gggtttgcgg
      661 agcgttctgt caatgatata atgcaaggga acaaaatcac tggcttcagc gtctttttat
      721 gagagcccat tttcaagtga ggtaatacat tatgtctcga tctaaacttt tcaaagcgat
      781 atttgctttg gtcctgctgg tgactacggc ggcgtgcagc ctgacgaata cgacagtgcc
      841 actgccgccc acgctttcag cagaagacat gcaggcgacc gtcaatgcgg cggccacaat
      901 ctcaattcag acgattgcgg cgaacctgac cagtacagcc ctggcactgc caactgatac
      961 accggaaccc agtgcaaccc ccaccctgcg gccgtcgtcc acagttgcgc ctaccagcac
     1021 cccaccggcg gctacggtga cccccatcat aacccgggcg ccgacgcgca cccatacgcc
     1081 agtgcagtca ccgaccaaca cgcagcagcc aacatctgaa ggttataact gcaccatcat
     1141 ggctgtttct ccggcggctg atacggtgat cggcgcgggc aatgattttg acgtgcgctg
     1201 gacgctcaag aactcgggca gccagacgtg ggataatgcg gatgtggact atcgctggta
     1261 ctccggggat aagcttcaca ccggaaatga tatttatgat atggcggcag atgttgcacc
     1321 gaatgccacg gttgacatcg tcattgacgt caaggcgccg accacggtcg gcacttatgg
     1381 cgcaacctgg cggttgatgc gcagcgacaa ggtgatttgc acctttgact accgcttcaa
     1441 ggtgaagtaa accgtcaaac cattacacac ctgggccggt tttcccaaaa aaccggcctt
     1501 tttggtataa ttcgttcgtt taacccatcc aaattgttag agccgtaagg caggagatat
     1561 cgcttgaaca cccccaagaa accggctgaa attgccgatg atgtagttgt catgttgaaa
     1621 tatgtcctca ccgttgatgg cgaggaagtt gacagcgctg acgaaaaaga ccccatcgcc
     1681 tttttacagg gctccggaga aattatccct gggctggaaa aagcactgta tgggatgaaa
     1741 attggtcaga gcaagaaggt taccgttgcg ccggctgagg gttatggcga tcatgaaccc
     1801 gaggcgatga tgagcgttcc ccgcagcgaa ttcccggaag atattccgct ggaagttggg
     1861 attgagctgg acgtccgcga cgaagatgac gaagtgatga gcgccacgat tgtggacgtc
     1921 accaaagacg aaattaagct ggacttcaat catccgctgg cgggcaaaac cctgcacttt
     1981 gacatcactg tggtggacct gcgcgaagcg accgaagaag agctggagca tggtcacgtt
     2041 cacggtgacg atgacgacga atatgaggac gaagaagagg aatagacccc tctggcgggc
     2101 ggcgctgatg cgccgcccgc tttttttatt tttatgcccg gcggcgcggt atcatctcag
     2161 gcagatatga tatactgatt ccggtagctg ctcatggatg agattcgtca actcgaggaa
     2221 gcaattgcag ccttagaagc gcaacgcccg gtgctgggcg atgcggtggt tgagcttgcg
     2281 cttgattcaa tgctgcaaaa actggcgaca ttgaaagccg gacagggaga agcagagcac
     2341 gaagcccagc agcggaaaat ggtgacggtt ttgttcgccg atctctcagg gttcactgcg
     2401 ctggctgagc agatggatgc ggaagatatt accgacctga tgaatgcgtt atgggagcgg
     2461 ctggaccgaa ttatcaccca gcggcgaggg cggattgata aacacattgg ggatgccatt
     2521 atggctgtat ggggagcgga ggctgcacag gaggacgatg ctgaacaggc tattcgcgcc
     2581 gcgctggata tgcaggctga actgatgcgg ctgaataaca acaatgagat tccggggtta
     2641 aaagcgctgc ccgacgaaca gacgattgcc atgcggattg gcattaacac cggagcggcg
     2701 ctgctggggg tggttggcac caccggggaa attactgcaa ttggcgatac ggtgaataca
     2761 gccagccggc tgcaaaacgc agcgccattg aacgggatta tcatttcgca tgacacgttc
     2821 cggcatgtgc gcgggttgtt ccgggtgcgg gcattggagc cgctggcgct gaaggggaaa
     2881 gcggaaccgg tggcagttta tctagtagag aaggcacgcc cccacgcgtt ccggctggca
     2941 acgcgcggct ttgaagacct ggacacgcgc atggtaggtc ggggagaaga actggaacag
     3001 ctacaggctg attatatgga ggggttgaat gaacaaaacc cgcgcatggt cacgattatc
     3061 ggtgaagccg gggttggcaa aagccgcctg ttgtacgagt ttagcagctg gctggaatta
     3121 caaaaccacc tgacagcggc attcaaaatc cgagcaacgc agcaaaccag cagcgtgccg
     3181 gggctgctca tccgacagct gctcaccacc caactggaga tccgcgagag cgacccagcg
     3241 gaggtcatca tcgccaggct ggcgcatggg ttggagaaga cgcccggcag tgaagtgccg
     3301 ctgaacaacc gggcattaat cctggggact tgggcgggat ttgatttgcc tgatgacaat
     3361 gggttcattg gattgattgg ggcccggcaa ctgcgcgacc gcggaatgat tcacctggca
     3421 gaactattga gcgccagtgc agccgaggtg ccgctggcac tgctgctgga agatttgcaa
     3481 tgggctgatg agcagtcgct ggatgcaatt gaatttatcc agcgcgcctg tgcacatgtg
     3541 ccgctgttta cgatttgcgt ggcgcggccc gggtttctgg aacgccgccc ggattggccc
     3601 gccctctgca gtaccatgct ggaactgcgc ccgttgaatg aaaatgagag tcggacgctc
     3661 atccaggaaa ttttgcagag ggcccggcaa attccagagg cactgtgcag tctgattatc
     3721 cagcgatcag agggcaatcc gttttatatt gaagaactga tcaagatgtt gattgaagaa
     3781 ggtgtgattc agatacggga gcaggaatgg ctgattgacg caagccggct ggaaacactg
     3841 cccatcccgg caaccctgac cggggtgctg caagcccggt tggacagtct gcccgcaagc
     3901 gagaaattga cgctgcagcg ggcatccatt ttggggcgga ttttttggga ccgggcagtg
     3961 gcacagcttg gcaagacgga ggaccgtccc gaacaggatg aggcggtgca gaagccgctg
     4021 aacggactgc ggaaccgccg attgatttat cggcgcgaca aacctgcttt tgaaggcacc
     4081 aatgagttta tttttgtcca tgtgctgctg cgcgatgtgg cttacgagag tgtactcaag
     4141 agagaaagac ggcattatca tgccgaggcc gccaggtggc tgggtcaaac agcccgagcg
     4201 gccgggcggg ctgatgaata tgcggttttg attgccgagc attatgagca ggcaggagaa
     4261 atgttgagcg cgcaggaatg gtatgagcga gcggccggaa gcgccagcct gcgatacgcc
     4321 aatgcagagg cacggcggtg tctgacgcat gcactggcgc tgacacctgc caatgacccg
     4381 catcacagtt atgatttgcg gctggcccgt gagaaggttc tgaatttgct gggtgaccgc
     4441 ggcgcgcagc ttgaagatct aaatgcgctt gaggagattg ccaacgcgct gggcgacccc
     4501 aggcggcagg ctgaagtggc gctgcggcga gcgcattatg ccgaaatcac cggcgattac
     4561 ggcgaggcgg cggcacatgc cagccgggcg atccgcctgg cgcaggcggg aggagacgcc
     4621 agatcggaga ctgcgggaca cctgcagcat ggtcaaacac tgcggcggca ggggctttat
     4681 gaggctgccg gagaggaatt tgagattgca ttgaaacaag ccgaggctct gggagtgaca
     4741 gcgattgagg cggagtgcct gcacagcctg ggcaagctca gttttcatca ggggaactat
     4801 gcggcggctt cggattacta tgcccggact cgggtgattc acgagcagaa ccgggacctg
     4861 cagggcgtgg gggcggtttt gaacagcctg gggattgtgg ctgacagtca gggggattac
     4921 cccactgcca cacagtacta taccgaagcc ctgggggtct tccgtaaaat tggcgatctt
     4981 tgggggcagg gattggctta caacaacctg gggattgtat ccggtgaggt ggggaacctc
     5041 tcggatgcgc gcgattatta cagccaggcc ctacagatct gcattcagac caacgatcgt
     5101 tacggcgagg gcatggccta caacaacctg gggattattg ccgatgcgct gggcgattat
     5161 gggggggcac aacgctacta cgagcatggg ttaaaaatca tgcgggagac aggggggagg
     5221 caaggtgaag gggtggtgct ggcgagttat gggttgttga tggttcacgc cggcgacgcc
     5281 aatggggcgc tgacgctttc agaacaggcg ttggaaattg ccgatgaact gggagaacag
     5341 cgggttgcgg gttatgcgct gaccaatcaa ggacatgcgc tgctgctgct gggtaaaaat
     5401 gcagaagcgc tggcagccta tcaccgggcg gcgatgatcc ggcaggagct gggggacctg
     5461 acggtagcaa tcgagtcaca ggcgggcgag gtgcggtgtt taggccaact gggacgcagc
     5521 caggaagccc tgacgctaac ggaaaacata ctgaaacatc ttgaaaccaa taccctggac
     5581 ggcgttgaag agcccggacg ggtgtacctg acctgttacg atgtgctgca cgcccatcaa
     5641 gatgaacggg cagcctctgt gctgaagact gcgcaggaga atctgattgg caaggccaac
     5701 cgcatcagcg atgtcgagtc acggcgcggc tttctggaaa atgtggctgt ccaccgccag
     5761 attatgactt tatttacagg acaaggagat gctgcatgaa gctgattaat attccgccac
     5821 aaaaactcat cagcggagcc tatgcgctgt gggaagatca atggctgctg ttgaccagcg
     5881 gggattttga agaaaagcgg tttaatgcca tgacggtatc ctggggcagc ctgggggtaa
     5941 tgtggaaccg accctttgct caggtggtgg tgcgccccac gcggtacaca tttgaattca
     6001 tggaaaaata tgagaccttt accttgtgtg cgtttccatt gcagtaccga ccagcgctcc
     6061 aattgctggg ggcgcgctcg gggcgagaca gcaataagat gactgagtgc ggactgaccg
     6121 cggttgcagc atcacaggtc ggggcgccgg tttatgctga agccgagctg gtcattgaat
     6181 gtcagaaaat ctactggcaa gactttgatc ccgatcattt tcttgacccc aaaattggca
     6241 ataattacgc aggaaaagat tatcatcggg tatattatgg acagattctg gctgtctccg
     6301 ggatagaaca atatctggct gctgaggcaa aatgatgcca taacggaggg cagaatgccg
     6361 ctatttggcc cacccaatgt tgagaaatta aaagccgggg gcgatgtcct cgggttgatt
     6421 aaggcgctgg gatacgaagg cgatcgtggt gatacgcgga gagccgccat tgcagcactg
     6481 gggcagcttg gcgcggcggc ggtcccggcg ttgagcgagg cgctgcaaaa cagttcgctg
     6541 cctgtagcaa ctgctgcagc cgaagcgctg gggcgaaccc atgcacagca agcggtcaag
     6601 ccattaattc aactgctcag gcagcctaac cgctttctgc agatggcagc cgtgcagggg
     6661 ctgaaattat tgcccaccgc tgaaagcatg gcagcactcc aggaggcact gctgggcaat
     6721 gaggagactg cccgggctgc ggctgaagcg ctggcgcaaa tgaatctacc cgccgcctgg
     6781 cagagcctgg cggaaatgct gggacacagc gaatttaacg cccggatggc tgctgcacac
     6841 gggctggtag aagtgggcac ggccgggacg cctttgctgc tcgatgcgct ggaaaatggc
     6901 aatgaaaacg cacggcggtc tgcggcagaa cttttggggc aaatcaggcc gatggaggcg
     6961 ctggcggtac tgctcgaaat gatggccgac tcttctgatt acgtggcact ggcggcgggt
     7021 gatgctgttt ggcgatatgg cgacgcggcc atcatcccct tgctcaaggt gttgatccag
     7081 gggaatgacc ggttgaagac ggccgcagcc caacaattga tccgactggg ggaccgggca
     7141 gagcgcccgc tgctggcagc gctgcaaaac gctgaccccg atacccgcgc ccgggtggca
     7201 gtgattttgg ggaagattgg aagcggctgg acagtgcagc cattgatagc cctggcccgg
     7261 caaaccgacc cttctctgcg atatgcagcc gtgcaggcac tggctgaaat tggcggcgct
     7321 ggcgtggttg aggccgtgat tacggcattg gaggatgaaa acccatatat tcggagagcc
     7381 gcacgggagg cgctcattcg gattgaggaa acggctgaaa cgcgcgccct gcggaatcaa
     7441 attcaggggg ccttacaaag ccacccacca gacagggagt gacttgatga gcaaatcaat
     7501 acccgaacaa attcaggacc tgcgcaatgc cattgctgcg caggaaatgc tgcgccccac
     7561 cctgggggat gcaattgtgg atgcgacgat tgtggtgctc aatcaaaaat tgaccgcgct
     7621 ggaaagtgag ctgggaaaaa gcgaggagca gcgcaagctg gtgaccattc tgttcagtga
     7681 tattaagggg tctacggcaa tggcggagaa aatggacccg gaagaatggt ccgacattat
     7741 gaaacaggcc tttcaatttt tgattgaccc aatttaccgg caccagggat tggtggcgcg
     7801 cctgatgggt gatgccattc tggccttctt tggcgctgag acggtgcgag aggacgatgc
     7861 tgagcgggct gtgcgggccg gactggaaat tttgaccgga atgaagccct ttcaagaagc
     7921 tattgcccag cagcacggga ccgagtttgg ggttcgggta ggaattaaca cagggctggc
     7981 gctgttgagc aatgttggca ctgatcaggc aggggaattc acggcgttgg gcgatgcggt
     8041 gaatctggcg gcgcgcatgg agcagtcggc accgcccaac catttattga ttgcgccaga
     8101 tacttatgcg ctggtgcgcg agcgctttga aattgaagcc caggcgccga tcatggtcaa
     8161 agggaaaagt gagccaatac cggtgtacgt tgtggtgcgc gaactgccaa aatcaaccgt
     8221 ggaggtgcgg cgcgggatgg ccgggctgga tgttccgatg gtggggcgcg aggcggaaat
     8281 ggagcggctg cgggcgggct atcagcaggc ccgcgagggt aaggccaact gtatcctggt
     8341 cacgggagaa gcaggcagcg gtaaatcccg gctgcggtac gagttcaggc gctgggtgga
     8401 gcgccagggt gaggtagttc aaatgatgca agggcgggcg gatgcagcaa catggcatgc
     8461 gccatacagc ctgttacacg atcttttcgg caatgcctgc ggcattcagg aaagcgaccc
     8521 ggcggctgag gtacgctgca aactggagac cgggctgtcg ccctacctgg aggcagagcg
     8581 ggcggacctg gttgggcagt tgattgggtt taacctggca gacagtccag cagttcgagc
     8641 ctttgggcaa agcacggcct gggcacccct ggctgaggtg tatctggcgc aatatttcca
     8701 gcaggccagc cgggtgaggc caatgctgct tttattggaa gatctgcact gggcggacca
     8761 ggcatcgctt gattttctgc tggggctgcc caagcaaatg cccaccggtg ccttgatgct
     8821 gctggggctg gggcgaaccg agttactgga gcggctgagc gaggattgct gtgagaaaat
     8881 caacctgggg cagcttgact ccacagacag ccagcacctg ctggagacat tgattccaca
     8941 ggcagaccgt ttgccggagg catttaaggc aatggttatc ggacacgcca atggtaatcc
     9001 ctattatttg caggagattg ttcaaatgct gctggatgag gggttactct ctttagaggg
     9061 tggagccgtt atgagcgggc aggccgattg ggataatctg catttgcccc ccaccctgca
     9121 gggcgtgctg caagcccggc tggacagcct ttctgcggtt gagaagcagt ttttacagcg
     9181 ggcggcggtg ctgggcgagc agttttggga tgaggctctg acgcagctgc acaccgagca
     9241 ggatgcgatt gccgatattg agcagcccat tcacaacctg gaaaagcggc agttaatcta
     9301 cccggttgag gtttcggcgt ttgatgggct ggcagagtat attttcaaac acgcgctgct
     9361 gcggcaggca gcgtacgaca cattgctgct gaagctgcgg cgggtctatc accgacaggc
     9421 ggctcagtgg ttactggagc gatgcggcga ccggatgccg gaatacctgg gcaagattgg
     9481 ggatcacttt gagcgcgccg gggagaacga acaggcagcc ctgttctatc agcagcaggc
     9541 acgggatgct tacgcccggt cggcatttcg ggacgccctg ccgacggtgg aacgcgccat
     9601 tcgattgaca cgaccagaaa acgagccaca gttggctcag ttgcacttac aaatgagcaa
     9661 taaccttgaa aagctggggg aatacgacgc tgcaattgaa cacctgaata cctgcctggg
     9721 tctggcgcgg gggactggca gcacaggcgt tgaagccgaa gccctgtgta ttatggcctg
     9781 gattatggga attcgcgggc aagatgccga ggtagaaccg tacagccgga aggctctgga
     9841 gctggctgaa caggctgggg atatgcgtat tatggtgcgg gcgttaacac aggtggctgg
     9901 attgtatgag gccggtgagc caaccaaggc gctggaaatt tacgaacagg cgctggtgat
     9961 tgcccgcgag agccaaaatg cggcacaaat tgccaccaca ctgctcaaca ttggcaatac
    10021 tgaactgaac ctgaggcatc tggaggcggc tgagacgtat tacctggaaa gccaggcgat
    10081 ttatgaaaag agcgggaacc gctgggggct ggagaactgc ataaacaacc tggcgattat
    10141 ctataaactg cggcatgcgt ttgaaaaagg ccgggaaaac agccagcgag cgctgcaaat
    10201 tgctgaggag attggcgatc aggaagggca ggcactgtgc ctgatcagcc tggcgcactg
    10261 cgcgggtgga ctgcagcgga taaaagaagc acaagagcat ctgcgagcag cggttgagat
    10321 tattcagaag attgggctgg acctcaacct tgacctgctt aacatcatcg ggcgggtgct
    10381 ggtggcgcag ggccggtatc aagaggcggc ggagtatgca gggttggttc tgaccgagcc
    10441 ggacctgatt tttgaaatat ctcaggatgt tcacgattta ctggatgaaa tcaagatgca
    10501 cctgcctgag acgacaatcg aggcggcaaa attgcgcgga aaaactctgg accacgaaaa
    10561 cgccatccgg cggtttctgg aagaaagccg acaaaccgag taaaatagag ttcaggtttc
    10621 atcatgccag ccctagaccc cgtcctcatt tttgccattg ccaccagtat catttatatt
    10681 ctgctgttag gagccgtatt ccggcgcaca atcggcggcg accggtcgct gcttatccta
    10741 acggtggtat taatactggg gctatcgtgg tcgctcagcc tgacttttcc acggctggca
    10801 ttgttctcgg gtattcggat tgaaacgctt aagtaccgga ttccgttcta tcatattatt
    10861 acgctgacgc tgttgtttta tgcgctaaca cgcagcttta cccgccggca aattccgtgg
    10921 tggttctggg cgctggggct ggcctggctg gtgttgacgg taatcatata cgagaatgtt
    10981 ctggggacac cggaagaatg gtggctgggg ggaaaccgta ccttgccgcg aagtatgggt
    11041 aacaccctga tcatcggacc ggtgtgggct tttctggtga tcagcggcta tgtgatttta
    11101 gtccgggaat acaagcaatt aaccagcccg ctgcataaaa accggttccg ctattggatg
    11161 ttaggtcaaa ctttgatggc tattgggggt ggactggccc tgttcaatca aaggattgtg
    11221 gggggaacgg ccttcttgtt agcgaatgtg gctgtcactt atgtgctgac gacctaccga
    11281 ccctttgatt tacgcgaagg cgccgggcgt ttttctgttt ttctcatcaa tacagttctg
    11341 gctgtgggta tatattcggt tggtttttgg gcggcaagct ggctggtgca cctgtattac
    11401 acgaccaatg cctggttgat ctgggctggg atgggtgtgg tgctgaccct gctggtttcg
    11461 ccactgctca tgcgcaccca tcggtggctg acggatatga tttttggcag cggatacgat
    11521 gcccgcgatg cggtgcggga atattctgcc agcatcaata atattctgag tctggaacaa
    11581 cttaccaatg tgattaccgg ttttatgcag gatactttcc aggttaagca ctctcagctt
    11641 gtgctggtga actttgaaga ggtcaaagcc agtgacccgc cacagggata ttatctgttg
    11701 aaggttgtgg ccagcgggga gggacttccg accgtggcaa ttatggcaga cgacagcccg
    11761 gtaggtgaat atctgcggaa tgcccgcatt ccgctaacgc agtatgagat tgatgtgctg
    11821 ccgcgcttta gagaaaccgc aggacccgag cgcacctggc tggctggact gtcggcagat
    11881 gtctatgtgc cgatcttctc caaggggcag tgggtggggc tgctggcgct gggggccaag
    11941 acctcaggag agcgttttta tgataatgac ctggcgctgt taagtacgat tgccgaccaa
    12001 accgcggtgg cgctggaaaa cgcccgcctg tttgatgacc tgcgccagcg gaatgaggaa
    12061 aacgagcatt tgaacctgga attgaccgag gcctaccagg aattggagcg gcttgaccag
    12121 gcaaagtcca acttcctcaa cattgcctcg cacgagctga gaacaccctt gacccaggtg
    12181 cggggttaca cagatatgct caacgaaagc atcagcgaga agaatattac gccggagact
    12241 gccgggatta tggcgcaggg tctgcgcaag gcgacgctgc ggttggagag cattgttgat
    12301 attatgttcg atgtctcacg ggtagaaacg gggacactgg aactggacct ggtgccgctg
    12361 ggcatggcca gcgtgctgca actggcgaca gacgcctggc aggatgctct gcggcggcga
    12421 aagatttcag taaatattgt aggcgtggac aacctgccga ccattcaagg cgacctgcaa
    12481 cggctgaaac aagccttttc caatgttatt caaaatgcga ttaaatatac gcccaacggt
    12541 gggcatatcc agatcagtgg gtatttgctg accaatccgg agaatccgga agagcaggcg
    12601 gtggaaatta tcatcgctga tacggggatt gggattgagc cagcggaact tgaacgaatc
    12661 tttgatgcgt tttatggcgc aggggatcca aacctgcaca gcacaggcga tttcaagttc
    12721 aagggcggcg ggcccgggct ggggttgacg attacccgag ggatcattga cgcccacggc
    12781 gggcgcgtgt gggcggagag tccagggtat gatgaaactc attacccggg cagcaaattc
    12841 catattctga tcccggtcaa cccaccgacg ccaccgctgg cctgagacag cccatgtttt
    12901 tgccatccac ccgatctgaa attgaagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnac gcaaaaaaag cggcggcagg atgattatac gccggggggc gaaaacaacc
    13081 gccgcccgga ccgggcagtg attgcatatg ccaatctgat ccggcggtat tttaagggca
    13141 cccgaccgat tgtactgggg gggattgaag ccagcctgcg gcgagtggcg cattatgatt
    13201 actgggatga ccggatccgg cgggcgatat tatttgatgc caaggccgat tacctgcttt
    13261 acggcatggc agagagcggt gtggttgccc tggctgaagc actgcgagag gggcgttcgc
    13321 cgctggatct gcgcgggtta tgctacctga gcaaaacgcc tccggcagaa gcaattgaac
    13381 tacccagcta tgaagaggtg acggccgata aactggcgtt tatccggatg tttcacacgt
    13441 tttatcaaaa caatgacgcg ctgacggccc ggacattatg ccagcgtcat ggcgaccgat
    13501 atctggtaca gaacccgccg gggatgcctt tgaaacaggc agacctggac gccatttttg
    13561 ccctgccgtt tgagcgcaag gttcacccct ttgacgccca gggcgggccg gtgcgggcgc
    13621 tggagacgat ccgattttca atttcaacgc accgcgggtg ttacggcgag tgtaatttct
    13681 gcgcaattgc agtgcacgag ggacgaacgg tgcaatggcg cagccagggc tctatcgttg
    13741 atgaggctac acgcattaca catctgccag ggttcaaagg gtatttgacc gatgtgggcg
    13801 ggccaaccgc caacatgtat gggtatgagt gcgacaagaa gctggggatg ggcagctgcg
    13861 ccaaccgcag atgcctgaca cccgagatct gccgccagat gcggcccgac caccgtaaac
    13921 aaatgcaact gctggaagaa ctgcggcaaa ttccgggaat gaagaaggtc tttgtagctt
    13981 cgggcatccg gtatgacctg gtgctggcag accgaatgca cggacgggcc tatctgcggg
    14041 cggtggtcaa tcaccatgta tcgggtcaaa tgaaggtggc gccagaacac agcgaggacc
    14101 gggtgctgcg gcagatgggg aaacccggca atgacagtct gctgcgcttt aaggcgatgt
    14161 tcgagacatt gactgctgaa gccggtctgc cccaattctt gacctattac ctgattgcgg
    14221 cacaccctgg atgcacagaa gccgacatgc ggcggatgaa ggacttcgtc agcagcaagc
    14281 tgcatatcac tccggaacag gtgcaggtct tcacccccac cccatccacc tatgccagcc
    14341 tgatgtacta taccgagctt gatcccttta cgctccaacc gatttttgtg gagaaaaatc
    14401 tgcacgccaa agaggtccag aaggagatca tcaccgggaa aggacctcaa ccgccacagc
    14461 ggagccaacc actccaacgg gacaagcctc tgccgccaaa gaaaaaacga tcatgaaaat
    14521 tgagaacagg cgcatcattc taaccggggc ggcctccggc attggccggg ccctgctgga
    14581 ggccttatgc cagatcccct gccacattct ggccgctgat cggaacggac cggcgcttgc
    14641 tgacagcctg gctgccctgg aagggtgccc agcacaattg agtgggctcg aggtggatct
    14701 ttcaaattcc gccggggttg atgcactgtt tgatgaggcg caggcacgaa tgaacggcgt
    14761 tgacatttgc atagccaatg ccggttttgc ttactatgag aaaatcaccc agccggattg
    14821 ggcccatatt gagaccatct accgattaaa tgttttttct gccatttatt cgctagaaaa
    14881 actgcagttg ctggctgccg gccacgattt tcgcatggta atgattgcct cgggcatgtc
    14941 tcacctggct gttccaggat atgctatcta tggatccacc aaggcggcgc tggatcgatt
    15001 tgcagaaggt ctgcgctatg agctagcccg acctgaacaa ttgatgctgg tctatccgat
    15061 tgcaacgcgc accaattttt ttcaacaggc tgcccacaaa accccggtcc catttcccag
    15121 ccagacacct gaggtcgttg ccacagccat tctgcgcggc atccggcgag atcgacgccg
    15181 ggtttatcct tccgctattt tttcgttcat tttactgcta gaccgttttc tgccattgac
    15241 gcgccggctc tatcaggcgc acgaaaacaa agttttcaaa caatggcaac agggtcaaaa
    15301 ctaaccaagc tgctgcctta tgcgggaaat ttacacggct tttggcagcc aatcccattc
    15361 caggagctct catgaatttt atagaagatt tgcttgcccg actgccagat tggtttcagg
    15421 atgaagaaca gttcaagctt gcccatctga cccatcagct gtacccatac actgcgctct
    15481 ttacacctat tcagatcaat cagttaaccg tcaaaaaccg aattgtgatg gggcccatgg
    15541 gcaatatctc tgttgctgat gaacatggac ggccatccag taaaatgatc cagtattttg
    15601 ccgagcgtgc ccgaggtggg gtgggattga ttacttcggg gttggtgcca ttcagccaag
    15661 ggatcgaccc ttcggtcact gaggccgatg gccgcagcta cttcccccgc attgagggat
    15721 cgcgaaccgt ctatgcaggt tggcgggatc tggcagaatc gatccatgcc tatggctcac
    15781 gcttttttat tcagctgaca cccggattgg gccgggtcgg gtcaccagaa gtcttgatgc
    15841 acaaatacaa gctgcccgtt tcggcttcct ggaatcctaa tttctatatt ccagctgtgc
    15901 cctgccggcc gctcaccgac gccgagtgcc gtaaaatcat tcaacggggc ggacaggcag
    15961 cggctgacgc caaagcaatg caaattgacg gtgtttatct gcacggacat gaaggctatc
    16021 tgctggaaca gatgaccaat cgcgccttca accggcgtaa actggggcga tatgccgact
    16081 ggcaggcttt tggcattgag ctggttgaaa ccatgcgccg ccgcgtaggc ccccactatc
    16141 cgatcatgta tcggattgat ctatcgctgg cattaaatgc cacctaccag ggaaaaatgc
    16201 agacggaacc caccctgcgg catttccgcg aggagcgcag tgtggaagaa accctggcct
    16261 acatggtgaa tctggtgcgg gccggggttg actgttttga tgtcgacctg gggtgttatg
    16321 acaattggtg gctgccacac cctccgaact ccatgccttc gggttgctat ctggcggttg
    16381 cccaactggt caagaacacc tttactgcgc agcatatcca gtccaatgcg ggactgccgg
    16441 tgccggtggt cgctgtgggc aaactaggat atcccgatct ggctgaaaaa gccctgcggg
    16501 atgggctgtg tgacatggtg atgctggccc ggcctctgct ggcggatccc gattgggcca
    16561 acaaagcctt tgccggacag gttcaatcta tccggccctg catcggtgat caggaagcct
    16621 gcatcaacga atttattgaa ggcgggcaca gccagtgtgc cgtgaacccg cgcaccggat
    16681 ttgaggatat catcgatcgc gatccctgtc cggctcagca gatcaaacga gtggcggttg
    16741 taggggcagg cccggcgggc gtgatctgtg caaccaccgc tgcagcccgg ggacatcagg
    16801 tgacgctcta tgaaaagcga tctcagattg gggggatgct ggtgccgggt tccgtgccca
    16861 agatcaaata tgaggtcgcc aactatctga cgtttctgaa aaacgagatt caggacagtt
    16921 gcgaacgcca taacctggcc ttgaagttga atacagaagt cagtctggcg atgctgcaag
    16981 acggcaagtt tgatgccatt gtcatctgta cgggcggcac ccccgccatg ccccccattc
    17041 caggcgtgga tcatccagac gtcatccagg ctgtggacct gttcctcaat ccacagctgg
    17101 cggaaagtgc ggaaaaagtt gtcatcatcg gcggtggtgc ggttggttgt gaagctgctc
    17161 actatctggc tggcgaatac aataaacagg tcacaatcat cgagatgctg ccagttttga
    17221 tgaagggtgt ttgtacggcc aatcggggcc atctcgtcca cgaaatggag aagctggggg
    17281 tgaagatctg gatccacacc cgtgtaaaag ccattgatgc cgggcgggtg accgttgagc
    17341 gcaatatatc ccccacaacg cccgacccgt acaatacctg gacgccgctg ctacccgata
    17401 atattcccaa tccgctggcc cgccccatcc gggaggtctg gcaaactgag tttcttgatg
    17461 ccgacctggt tgtgctggcg gccgggctgc gccgcaacac cgcgctttat gaaaattgca
    17521 tacaggccaa tgcggcgcca gagatccata ccattggaga tgcattccag atcgggcgag
    17581 tgtttgaagc ggtaaaggct ggttttgcca ttggccggtc actctaagac cgaaccgtcc
    17641 caacggttat ctgataagaa ggctcatcct gaaatttcaa caaaccgccc caatggggcg
    17701 gtttgtcata atttacaatt ggatgtcggc gatgggccta tttacccatg gcctccatgg
    17761 cggtgatctg agcctggcga gccaggtcga tggaggttgc caggatacgg agcgcttctt
    17821 cagggttatg gaagcccatg ctgttctcgg cggcgatgaa atcccaacgc agttgggcgg
    17881 agcgatggag caggcgggct tcggcgagtt tatcggcatc cacgccatcg gaggcggcgg
    17941 cggtagaaat tgcgttaatc gcggcgacaa tcgcatcttc tgaggcgctc atggtctgcc
    18001 agacctggcc ctggatttct tcaacgcggc cgaccacata gtccacatca gtatggcagg
    18061 tgccgcaggt ggaggcggga ttgaagagcg ggctgtgcac atcgtgcgtg gagaacttga
    18121 cggcgccatc acgggtgtag ggcatgtggc agtcggcgca ggcaacgcca gcttcgtaat
    18181 gggtgctgcc agcggagaag agttcgtaat cggggtgctg catcttgatc atatcggcac
    18241 cgctgtcggg atgggtccaa tcaacatgac catcttctac atagtaggct tcgatatctt
    18301 caatggtggt gccattggtc caggggaagg tcagatagtt ggtgcctttt tcaaagtaat
    18361 attcaacatg gcagttggcg caaaccaccg tgcgcatttc ctggcgggtg aaggtctgcc
    18421 aatcgaggcc ctgggcgacc agagcgtttt cgagggcggg gttggtcacc accaggcgca
    18481 tggtgccggc ttcgtggcag ttggcacagc caatgggttc ggtaatctgt tcgccaagct
    18541 gggcaaacgg catggcatca taggcttcca tgcccatttc agaccagagc tggggattgt
    18601 tggaggattt gcaggagtag caggtgccgg gggtcttgtc tgagaggcgc ttggtggcgc
    18661 gcacatccgt cagggcgttt cggtgaccgc gttcttcgtt gtactctttg ctaaaggggt
    18721 aacccgcaaa gagttcaacc aggcgtggat cttcttcaag cttggaaaag ggttcagagc
    18781 cgccataggt ggtacgggtg ttattgaact ctgtctggag cagggtggtg tattgattgg
    18841 gatagttgat gccccatttt gagctgtcgg gttccaaagg atcgactgcg acaatggtgg
    18901 gaacaccgcg agtttcgatg ggttgatttt tcatgaagat caacacacca atcaaaacgc
    18961 cagccaggat aacgatgagg acagacatcg ctatcagggt gaatttttga tctttaatca
    19021 gggcgggctt gggattcttc ttcacggcta tttatcctcc tccgacaata cagatggggc
    19081 atagatgccc ttatcttgat aaggcaaaat agaaatgccc cggtcgccat gggcaacgga
    19141 gcggtgacag tcaaagcagt agcggtcggt atcttcctgc cctaccgaga ccatggagac
    19201 ggtctcagag tgacagcgaa tgcagttttc ttgaatgatt ttatcggttt cttctttggc
    19261 gcgcagcggt tctgggatgt tacccagggt gaaatgggtg acatggttaa agcccgattt
    19321 tgctttaatc caatatttgg gaataaattc gtgaggggtg tgacagtcgt tgcaggtggc
    19381 tgcctgctgg tgaccggcgt gaaaccaacc ctcataaacg ccatccatca catggcagtt
    19441 gttgcaggtg gtgggatcgt tacccagata ggttatgaat tcggtagagt acgcgaagac
    19501 agccagcact gcaatggccg cgattccagc aatcaaaaga gggaggtatt ttttcatttc
    19561 tcaaaaattg gatagtaagg taaagatgtc tagattgtat caatttctgt gaaagcggac
    19621 aagtgatttt agccaccaat ttgatgattt aggtattaat cctccaccag gcgaggattg
    19681 aggatgcgtt cgagagcgat gcccaacatg gagaaggcca ggctggtgag cagcagaagc
    19741 gccaggggtt caagcaccca gaaacgggag ttgtagaggg ctccgtgggt caaagcctgg
    19801 tagatcaccg tgccccaggt gggatagcca gagttgatgt taaacaggcc cagggtggct
    19861 tccagaaaga cgaagccggg gatcaggacg accatttgcg ggatcatcac cgggataatg
    19921 cgaggaacca ggtactttga gatgatgcgc caactgctgg cgccataggt ctgggcggct
    19981 tcaatatagg tggcgtcttt gatctgtaga aacgcggcgc ggaagttttt ggttggcatg
    20041 ccgaagacat tcaacaacac cacaaccgtc agaacgaccc agagatcaat accgaggaag
    20101 gcgtaggcca agaccgcgat tgccagaatg ggcaggacca tgttggcctc ggtcagccgc
    20161 tggatcaagc catcaaccca gccgccgaac cagacgccgg cagcagccat gagcatggac
    20221 agtaaggtgg ttgccaggga accggcaagg ccaaacccca gggcgaaggg cagaccccac
    20281 aataatggca ccaataaatc gcggcggtaa tagtcggtgc cggcggcgcc atagacctgc
    20341 ccgagcataa caagtctgga ctcaatatcg ctgtctggct caaaggtaag gccgtcaatg
    20401 cgcagggtgt attggcctct gaccagcttg gggtgatcgg cttgaggatc ggcaaagagg
    20461 atgtaatagg cgggggtggg gaaaatatcg ccaaagatga accagttttg ccagttggga
    20521 ttttctgaga cgatgcgccg atgatggaga tagtcttcaa aagcatagga ggtttcgggc
    20581 gccactgata tgccctgaag gttgatctct cgaccatcgg gcgtggtcca caccatcgaa
    20641 atgtagggtt tcttctcttc gtagctgctt tcgaggtaga gaaattgttc tgaagggaaa
    20701 tcggcgtagg tgtagtcaaa tgtgtaggtc agcgtaatct gccacatgtc attgggcaag
    20761 gcccggacaa cccgttctgc ctgcccctgg gcgctgtcta ataccagggt tgagaggtaa
    20821 tcttgggagc ggaaaaggtt aaaccaggcc gggcgggcct gcttggggac aaccgcctgc
    20881 cctgtcagaa tagttcggcc ccactcacgg ccaatcttct cgtggggcag ggcaataacg
    20941 gcataaagcg agccgataat catcaaagag atcatggaca ggccaatgat cgccgtggga
    21001 taacggtgga tttcgcgggc agcgcgcttg agccaggcga taatattgca aatggcaaga
    21061 tggaagttgg ttttccagtc cgcccaattg aggcgcggat ggggatgggg gaccggtggt
    21121 ctatcgaggg aatgggttgg tgcagtacga cgcctgggga accaggagaa accgccctgc
    21181 cggtgcaaag ggcgcaaacg caaaactgag tcagactgac ccaggcgaat gcgggggtca
    21241 atcaggacgt agacgatatc cagaagaaag acaaccgacc ccaacaaata ggcgaacatg
    21301 accaccagag atacggcgat tagcagttcg ccagggtaca tgctctcgcc ccagaagttg
    21361 ggtaagccga catcaatgta gagcaggcca atgccgggcc agcggaatat aacttccaga
    21421 gccatggtca tttgccagaa ggaaaccagc atgagcgaaa agctggtaat gacataagat
    21481 agtgagggtt tgaggatgta tttgcgttgg aggtgctgcg gtgaaaagcc aaccgccttc
    21541 cccagggtaa cataatcttc ttctgaaaag atgacaaaga aggtgcgcca ggtatagacc
    21601 agttggaaga aaaggctgat gaaaatagcc aagaccggca gaatcatatg tttgaggacg
    21661 atcaggatgt agccggtcca atgagcgggg gggagggtat ccagcatgcc cgagggcggc
    21721 aagagcttaa gttcagccga aaagatagca atcagcaaga tgcccacaac ccaacttggg
    21781 atggatgaga tgggggcaat aacggacagg gctcggtcta accaggagcc gtatttattg
    21841 gccagccgca gggccaacgg caaacccagg aggaagatca gcaggttaga ggaaaccacc
    21901 agaagcagcg tattgggaag atactgcaaa aggatgtcat taagatctaa cgacagctgg
    21961 ctgcgattaa acatggtcaa cggtctgacg ctgaccacct gaagacgacc ccaatcaaac
    22021 ttcagggcct tgagggtcca cagcaggtga cggggaaggt agggcagatc caggccgact
    22081 tcatcaatca gttcggcgcg caagctggta atgatctcgt cctgttctgt ctctgaaaga
    22141 tcgacaaagc cgggcgtctg aacgcggtac gaacgcaggt actgttcaat atgattggct
    22201 aaattcatgt caagctgggg gggcatggtg ccatatccca actgcacagg ttggttgaca
    22261 atcaggatgg ttataaaaac gccaacaaaa atggtggaaa agatgagcag gccgcgtagc
    22321 agaagatagc gaacagcccg cagaaaactc cgcaggccag aagcacccgt tatcacgggg
    22381 gcagtatttg gaatctctgg gggttgaggt gtggggtggg aagaaaaatc agtggtgcgg
    22441 ctcatgggtg gtaattccag atacagttcc attgtacccc gataagctta aggatttgtt
    22501 aggaaaacag aaaaccaaaa cccgggcatc tattttgcgt atataatagg aacgttctgt
    22561 atcctgcctg aggtcattgt tatcttatga aacgtaaccc attctataac cgccagagaa
    22621 ttaccgatgc ggccagtttt attggccgga aatcggaaat tgagacactg tacagcgcag
    22681 tggtcacccg gcaatgccgc tcactggttg gtgagcgcaa gatgggcaaa tcctcgctgt
    22741 tgacccatat cagtaatcca aacacaatgc gcgagtacgg gctggacccg gaccggcagc
    22801 ttttgctgta ttttgacctg gagggcatgg cttcggctac ggttgaggat ttctggctgg
    22861 aaattttgga agcgatctac gaccggatgc ccgagggcga actggcagag tcggttcaca
    22921 agctgataag cagcggcgac gtgcgcttta tggcggtgcg gcggctgctg cggcggattc
    22981 gagatgccgg gtttgatctt gtgctgtgcc tggatgaatt tgaatcactg gcgcgcaatt
    23041 cgcgatttga gccagacttt tatggagaac tgcgcagcct ggcgggtgag ctggggctgg
    23101 tttatatcac ggcctcaaaa cgcagcttat atgagctgac ttacgaacat acagataccc
    23161 tctcctcccc gtttttcaat attttttccg aactgccgct gggctctttg gatgatgacg
    23221 aagccaacgc tctgctgaca acactttcag ccagcgacgg cggcccggct ttttgtgcgg
    23281 aagaaattgc ctttgcgcgc gaactggcgg gggggcatcc tttcttcctg caaattgcgg
    23341 gcttccacct gattgaaaca ccagggcgcg gtacagcccg tgccaacgaa ggctatgcgc
    23401 aaattcgcaa gcgctatctg gccgaggcgg aggatcatta tcgctatttg tggtcacagc
    23461 tctcctccac ccaacaaatg gccttgatac acctggaaga tgcacccgaa ccggtgctgc
    23521 ggatactgcg gattaagagc ctggtggaag agcgcaataa taagccggtg ccattctgtc
    23581 agacgtttgc tgaattttta cagaagcagc gaaccgagca gggaactttt caaagcacag
    23641 cagccccaac cgggggcagt gatttgattg gacggacgct ggggaattac cggattgttt
    23701 cgccgattgg gcagggcggc atggctgagg ttttcaaagg ctaccagccg actctggatc
    23761 ggtatgtggc tgtcaaggtg ctgtcgccgc ggtttgcggc tgaccctgaa ttttttgagc
    23821 gctttcagcg cgaggcggcg gcggttgccc gactgcacca tcccaatatt gtgcaggttt
    23881 ttgattttgg caatgccgaa agcctgacct acatggtgat ggaatatatt cccggaccta
    23941 cgctgaaaga gcgcattcga actgtgcgcg gcctggggca gcagatgagc caccgcgagg
    24001 tggttgcggt gacccaacag attgcctcgg cgctggacca cgcacatgca aacgggctgg
    24061 tccatcggga tgtgaagcca gccaatatcc tgctgcgagc cacggatttt gagcaagcca
    24121 ccctgacccc ctcaacatct actcaactgg tgcagaacgg ttatgcggtg ctgaccgatt
    24181 ttggggtggt taagatgctg gaagggctaa aattcacggc gactggaatg accatgggaa
    24241 cgcctgatta catgtcgcct gagcaggcgc ggggcgagga tgtgaccagt tcatcggata
    24301 tatatgccct ggcagtggtt gtttatgaaa tgctggtggg gcaactcccc ttctatgcag
    24361 atacgccgct ggcggtgctg ctcaaacata tgaacgatat gccgcccaag ccgcatacgg
    24421 ttctgcccga actgccgatt ggggttgatc tggtgctggg gcgggcgctg gcaaagaccc
    24481 cggcagaacg ctacgccacg gcatctgatt tcagcaatgc gctggaggtt gtactctcat
    24541 gacacgtcga agcatgatca gcggtgtgtg ggtcgggctg ttattaacac tcacaggtct
    24601 gtacccggct ttttatttat ttgtggggcg ggttgtgatt gaaattggca atcacttgca
    24661 gcggcagttt ggcaatttta aaattggcgt gctggatgtg ccggtatctc aaattgttct
    24721 gctcgtgacc accgggattt gcatgatgct gctgctgttg gtgggcagca tccccaccct
    24781 gcgcagcggg gcacgcggca tcaaacaggg cgttaaattt ggcgtgattg gcagtatggt
    24841 ggctgggtta acggtttatt ttatgctcat tgcccccacc agcgcgattg tggcaggcaa
    24901 agacctgttt gcccaccgct tgagcgtgat ggcagcccaa tatccagaac atatggtggt
    24961 caattttgcc cgggatgtga ttgtggtggc tccgggcagg atgctgatca cactggcgtt
    25021 gtttgccctg gtcggcgcag tagaaggcgg gctggtgggc tttctgcggc gcacccaaga
    25081 cccggccaag agaccaactg ccctattaga cgtgcccagc accctgcgcg gacatgcggc
    25141 ctggtttgca gaatctcagg aagcgctggt atcgggagca ttaacggggc tggcattagg
    25201 ggccttgatg atgctggcaa gcatctgggc aattgccaat gcatcgggcg caacggctga
    25261 agaaatgtgg ttgatggatg tgacccaaaa ggcgctggcc gggacgccga ttggcagaat
    25321 gatggacacg ttgattgcca atctgttggt gcccatggta tttgtattgg gaacggttgc
    25381 cggtggactg gcagccttct tccccaaaaa cccgccggga cggctgggac cgcgggtggt
    25441 ggcggcagcg ctggcaggag cggtatcggg gatgatcttt ggcattgcac tttcggatca
    25501 aacccggttt gcgctgatca tctccaatca gtttatgtat tcaaacctca tttcggttca
    25561 ggttgatccc agtatgccct gggaattggg tgagttcatg accgttttgg cgactgcgcc
    25621 catccggaca ctgctggttt acctggtgcc actgccaacg acatttggcg tgaccctggc
    25681 gggtaccatc ctgggagcgc tgcaagggct gattttttcg ctcatcttca tggctgtcaa
    25741 tttacggcca acagaccggg cgtacaaagt tcgccgggag ttggaacagc acccagaggt
    25801 atttttaccg cgggtttata aactatttca gaacagccca atggcgctgg cggtgctgga
    25861 acatctctac tttgggctgc gccgcgaacc gacgaaggcc cggctgatcg gtgccttcca
    25921 tacaattgcc acgtcgccca cgcgcgccgc agatgcattg gaagcctcgg cgactattct
    25981 tagtgagcaa accggctgga agcagcatgc cgaaattgcg gcgctgcacc gcattctggc
    26041 gcgcgggttg aaagccagga cgattgccca actggcagcc atccagccca tgccggaaga
    26101 ccagacgaca tctttaccgc agccactggc aaaagcgggc gagttcgtgt cccagatcat
    26161 cagcgaggtc aagaagattg agcgcgtgga tgacttgagt gccaagatca tctttctgaa
    26221 caacacgctg gaaaccctgc gccaggggcg gattttcctg gaggcgacca aaaagcagga
    26281 ctgctgtgca agcacttatc ctgaactgat tgcattccac gctttcctgg atcagacttc
    26341 ggaactggcg ctggcgggtg tgcgggatat tcagggttgc gctgatttga tctcggagtt
    26401 gaaaacgcgc acgctgacca cggcagaaaa tcagaccctg acggttgtgc taaaaaacca
    26461 ggggctgaac gtggctgaaa acatccgcct gcaagtggac gatgccccgg gcctctatac
    26521 ggttgtggaa ggcgggcagc aaacggttgc tatcttaacg tcgcaggaac cgcgtgagtt
    26581 ttcgctgacc attcgcccgc tcaaaaacga gccgctgcgg gtgagctggc ggattgtgta
    26641 tgatgacgcg ctgaacaagg accgggtgct ggattttggc gaccagattg attttatgga
    26701 cgcgcagggc tcgttccaac gcatcttccc aattccttac gtgacgggga cgccgctgca
    26761 aaccggagag atgtttgtgg ggcgcgatga tatcttcgaa tttgtgcggg agaacctgtt
    26821 cggtacctat caaaataatg tgattgtgct gcatgggcag cgccggaccg ggaaaagctc
    26881 ggtgctttat catttgaatg atgtgctgaa agaatcccat atctgcgttt taattgacat
    26941 gcagggcaag gcggcgcgcg gagaagtcga ttttctatac tccattgccg atgatatcgt
    27001 ctataccctg gagaaccatg ggatcactgc ccccctgcct gaacgcagcg agtttgagga
    27061 ttcgccagag tttttcttcc gcagccggtt tttgcgtggg gtttatgagg ctttgggcga
    27121 gaagaatttg ctgctgatgt ttgatgagtt cgaggagctg caaaaacggg ttgaagatgg
    27181 caagttaagc gcggatatct tcccgtattt acgcaatctg atgcagcatg agcgccggct
    27241 ggactttatc tttgcgggca cacacaagct cgaagaactg gcatcggagt actggtcaat
    27301 tctctttaac attgccgcct ataagcggat tactttcctg gattacgacg acgtggtgca
    27361 gttggtaaca aagccggtgg aacgttacgg actgagttat gatcccctgg cgctggagcg
    27421 aattttccag gttgccagcg ggcatccgta ttttacgcag gtgatttgcc acgaactggt
    27481 ggcctatcat aacgataccg agcggaacta catcacaaca acctgcgtgg atgctgtgtt
    27541 ggatcggatt attgagcgcg gcgaagcgca tttcaagtat atttgggctg aatcgagcat
    27601 ggagcagcgc ttgctgttgg tggcgctggc agagctgttg gagacaatgg ataatgctac
    27661 gctggatgac attgccggag tgctggaaaa gcgcgggcga gccgttgaaa cgacggactt
    27721 aaatgaagct gccgggaaac tggaagcgcg cgatatttta ctgcgctcgg ggccgcgcag
    27781 caatctgtac cgtttccggg ttgatttgat ccgccgctgg atttacgcca cccgacctgc
    27841 ttacgaaaaa attcattaga aaagctgcct gggaagaggc gcatgacggg cttcttccca
    27901 ggcagaaagc cggggggtta cttcaaattg agcaggcccc aggtggaggg gtcaatcaac
    27961 gtacggtttt tggcgctgga gaccatgctc tgctggacgt tctcagattc cttgtcgttg
    28021 tcggagaatg aaatggcaaa gccgagggtc atgcctttgg aggggctgac gcccagcacc
    28081 gaccagggga tggaagcttc caggcgggtg acgccatcgc cgcgcacaga ggcaatgacc
    28141 acgctggatt ttgtgccagc cagattgctg ggataccaga ggtaagcctc gcgggtgccg
    28201 ttgacatcgg gtttgccaaa agaaatgccg agctggtaat catctgcgct gagttgcgtg
    28261 gtgttcaggt cgcccatcag gttggtgtca accagaattt caatgctgtc gcctttgaac
    28321 atatcagcgc cagcagcgtt ctggacatat ttttcatcca gtactttgac ggcgaggtag
    28381 agattctgat tatcccaacc gaggcggaac gagccttcca ggtcttcttc gccggtgcgg
    28441 ttattgccgc caaagaccac gtacttggcg gggtaggcgt tgttcttcca ttcatcccaa
    28501 acaccgtcaa tcgtcggcgg ggtggtcagg aagggggcat cgaaggtgcc gccggggcgc
    28561 agcggggctg gcgtgggggt ggggccggtg gtggcaattg gcggggtata tgcagttggg
    28621 gacggggcaa tgccaggctg tgtgggggcc acaacgggca gggttggggt tgggatggcg
    28681 gttaccaccg tcagcgtcgg ggtgttctgg gtaacggggg tcaactgcgc cagcgcggtt
    28741 gagaaagcgg actgggtaag ctgggcggat acggtctgga ccacagccgt ctcggcggca
    28801 gagttatcgg tctggttgtt gcccaccggg aaattacagg ctgcaagtac aaagagcagc
    28861 cctaacatta cgaaaattgg aagcgaaagc tttttcattg tcaaatctcc ttctccatgt
    28921 caatacatcc atgattataa cgacaaagca gtataaatag ttcccgaaat tttggggatg
    28981 caaggaagag acctctgaaa caggggaggt cagaccttgc aaactggttt agttatggga
    29041 taattatctg cctgtgatac ctgatttgcc tgatcatctg atcagggcaa tcgacaaagg
    29101 agcctgctgt gaaatttgag agtgtttgcg ttttggggtt gggctatatt ggcctgccca
    29161 cggcgagtac atttgcgacg aatgggctgc gggtcattgg ggtggatgtc aatccacaga
    29221 tcattgccgg gctgcaaaat ggggaactgc atatttatga gccagggctg cgcaccgtgg
    29281 ttcaggctgc gctgcgctcg ggcaatttgc gcatccgggc agaggtggaa ccggcagatg
    29341 cctttattat cgcggtgccg acaccttttt atgaagataa gtcggcggat atgcggtatg
    29401 tcgtttcggc aaccgaaatg attctgccgc atttgcgccg gggcaacctg gttgtgctgg
    29461 aatccacttc gccgccgcga actacgctgg atctggttcg cccgattttg gaacgttcgg
    29521 gactgaaggc ggggcaggat tttatgctgg cttacactcc tgaacgggtg ctgccggggc
    29581 agattctgcg cgagttaatt gaaaacaccc gcatcatcgg cggcattgac cgggcctcgg
    29641 ctgaggccgg gcgggatctt tacggtacat ttgtgcgcgg ggatatcctg ctgaccgatg
    29701 ccaccacggc agaaatggtc aagctgatgg aaaacacaac ccgcgatgtg aatattgcga
    29761 ttgccaatga atttgcgcgc ctggcagatc ggtttggcgt ggacgtttgg gaagccattg
    29821 gcctggccaa ccggcatccc cgggtcaaga ttctcaatcc ggggccgggc gtgggtggtc
    29881 actgtatcag tgtggaccca tggttcttcg tggatgcggc gcccgacatt acacccttaa
    29941 tcaagacagc gcggcaggtg aatgactcac agccgcattt tgtgattgat tggctcaccc
    30001 gtattttggg gccactgagt gggcgaaaag tcgccgcact ggggttggcc ttcaagccag
    30061 acgtggacga tctgcgcgag agcccggcga ttgaaattgc gcacctgctg catcaggctg
    30121 gcgcgcaggt tgcctgcttt gacccattta agcccgatgc tgaactgccc ggactgaaaa
    30181 cctgccagac catcgaagca gcaattgccg aggctgaaat tgtgatgatg ctggttgggc
    30241 atgcacagtt acgcgggctg gaccccatgg ccctgcggga gcaaaccact gcgcgcattg
    30301 cggtggatat ggtcaatggc tggcagccgg ctgcctggca agccgcgggg tttgatcttc
    30361 accggctggg agttggacac agggggtaaa catgaagaat ttacgagtgt taaccatctt
    30421 tgggacacgc ccagaggcgg tcaagatggc gccggtggtg ttgaacctgg cccgaacgcc
    30481 ggggattgat gccaaggtct gtgtgaccgc acaacaccgg cagatgctgg accaggtttt
    30541 gaatattttc aatatccagc cagatgttga ccttaatttg atgcggccca accaggggct
    30601 cacagagctg acggctgata ttttgacccg gctggacccg gtgttgaagg acctgcatcc
    30661 ggactgggtc ttgattcaag gggatacgac cacagttatg gtaaccgccc tgctgtgcta
    30721 ctataaccgc atcaagatgg ggcatgttga agcgggactg cggacgggcg ataaatggca
    30781 gcctttcccg gaagaaatca accgaaagat tgccggggta gctgccgacc tgcactttgc
    30841 gccaaccgaa cacagccggc agaacctgct gcgagaaggc gtgccggatg agcagattat
    30901 tgtgaccggc aacccggcga ttgatgcact gcaaatgatt gtgcagcgcc ctgccccacc
    30961 agaagttggg cagttatttg ccgatcacga tattgggcca gggaaaaaag gtctggtgtt
    31021 gattacggca caccgccgcg agaactttgg aaaaccgctg gagaatatct gcacggcaat
    31081 tcgcctcctg gctgagcagt acgccgaccg gctgcatttt gtctggccag tgcacctcaa
    31141 tcccaatgtg caggaaccgg cgcaccgcct gctggatcag gtctcaaatg tgacgctgct
    31201 gccaccagca gattacctgc cgatggtgca tctgcagcgc catgccacgc tggtgctgac
    31261 ggattcgggg gggattcagg aagaggcgac tggactgggc gtgccaacgc tggtgatgcg
    31321 ggaagtgacc gagaggcccg agggtgtgga agcaggggtt ctgaaactgg tgggcacaga
    31381 gacagagcgg attgttggcg aagcgcagcg gctgttggag aacccggaag cctatcaagc
    31441 aatggcacat gcagccaatc cctttggtga tgggcatgcg gcagaaagaa tcacgcaggc
    31501 gttgttgaag caccatgact gagagcatct gcatcttacc caacaccacg ggactgggtg
    31561 gcccggcgtc ttttcaagcg cggctgattg cagggctgca tgaacgccat atcccggtgc
    31621 accacaaccc ggatgatcca acctgccgaa ctgtgctggt gattggtgga accaagcggc
    31681 tggatttatt aatcaaggcc aagcggcgcg ggctgcggat tgttcaacgc ctgaatggga
    31741 tgaactggat tcaccggaag aaagacaccg ggattccgca ttatctgcga tctgaatggg
    31801 gcaactggct gctttccaca atccggcggc gattggctga cgagattatt taccagagtc
    31861 aatttgcgcg cacctggtgg caaacagtct atggacaggt taatgcccgc gggcgggtca
    31921 ttttcaatgg cgtggatttg aaggcattca cgcctgaagg gaaacacaac cggcctaaag
    31981 acgggctgcg aatactggtt gtggaagggc atctgcgtgg ggggtatgaa atggggttgg
    32041 aaactgctct ggaactgtgc aaggcgctgg cgcccaggct gcagcagccg ctggaattga
    32101 tggttgccgg agatgttgac cctgacctga aagattgggc gcagcaacgg acggcagtgc
    32161 ccatccgctg ggcgggagtg gtgccgcgaa ctgaaatccc cgagctggat cgctctgctc
    32221 acctgttatt ttcggccgat ttgaatgcgg catgtccgaa ctcggtcatc gaggcgctgg
    32281 cctgcggact gcccgtggcg gcgtatgcca ccggatcact gccggagctg ctggcagacg
    32341 atgccgggcg ggttgccaat tatggcacaa attattggga gcttgaagcg ccggtgattg
    32401 agaacctggc tgatgcggca gaagttattt tgaaggatga aatgcatttt cggcaagccg
    32461 cccggacacg cgccgaggca gcctttggat tggacgccat ggtcagccag tatattgaag
    32521 cgctgctgcc aggacgataa aaccttttac tctttaca
//
LOCUS       DAOPVS010000016        28695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1597_length_28695_cov_57.848568, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000016 DAOPVS010000000
VERSION     DAOPVS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 28695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28695
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_28695_cov_57.848568"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1033..1506)
                     /locus_tag="PKL11_03010"
     CDS             complement(1033..1506)
                     /locus_tag="PKL11_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNZ12600.1"
                     /translation="MKPATFNWAGMKNVQKIHRVLYAIGLGPLIGKIILLLTTTGRRS
                     GKKRVTPLQYELIGADYYLGAARGLKADWVRNIQSNPQVEIRVGTKCFQGMAEVITDP
                     SKFADLMEVRLARHPHMVGLIMERAHGLPRHPSREQLEELAKREAVVVVHPTNFN"
     gene            complement(1743..2357)
                     /locus_tag="PKL11_03015"
     CDS             complement(1743..2357)
                     /locus_tag="PKL11_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12601.1"
                     /translation="MTKNLETLAPRPETIKHLQAAFPSYLSKDVEAVVAILPTCFLEP
                     TQYDVGPITISGELIHIPSRIYSPEPRLASLYGLSAQELAILACIYTRHHDGFIRQKY
                     LEKLFSQDEIWVCPFVLQPIGEYVVEIIEVILHNINRMPMQSYHKFINENPAFVYLLK
                     QRIVSYWNCYYRSRFPHFEHYVGFQIANMLGLWNKNDVRHLLAR"
     gene            complement(2393..3304)
                     /locus_tag="PKL11_03020"
     CDS             complement(2393..3304)
                     /locus_tag="PKL11_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12602.1"
                     /translation="MKQPVFMLIPNFLKDPKAFYQSVQRNENLKGKALGLLVSIVVFL
                     MLYGFVTGLSHSWLQGLSTAIKMPLLFLLTLIFTLPALYFFALALLNVRFSVAQAGII
                     VLSGIGVSAFLLLGLSPVTLFFVLTSSNYAFFQLVAVIFVAISGVTGLYYVLNGFKWV
                     DQNREMANSSLGNALLRMWVVLYGFVGAQMTWRLSPFIGDPLTPFEIIHPSRDNFFVD
                     VINAIKRATGMNTTTATEIDFVAALFCGGALLALAIGVGIWLGSRKKAPTAAPAPSPA
                     VQAAMVPPAPAEGGGNPPVDGGASPKQ"
     gene            complement(3387..3785)
                     /locus_tag="PKL11_03025"
     CDS             complement(3387..3785)
                     /locus_tag="PKL11_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNZ12603.1"
                     /translation="MEIPRHWRLKKQRYALVGETCPHCDGKIFPPRDVCPHCGGEAKT
                     PYGFSGRGKVFSFTMMQDAPAGYEAQAPYTVALVQLEEGPVITAQLTDLGDQAVEIGM
                     PVEMVTRKIRQDGNDRGILVYGYKFRPLMQ"
     gene            complement(3855..5030)
                     /locus_tag="PKL11_03030"
     CDS             complement(3855..5030)
                     /locus_tag="PKL11_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNZ12604.1"
                     /translation="MRDVVIAGIGQIPVGEHWELSLRTLAARAMLAARHEAGMELKPQ
                     ALYIGNMLASTVSHQSNLGALLADNTGLTGIEAFTVEAADASGAAAFRMGYLAVASGM
                     VDTALVVGVEKYTDMVGSELEAAVSEMTDYDWEAVEGLTPTAQAGLLMQRYLYEYRPP
                     REAFAAFPLLAHANGAGNPNAMYRKTIKPEVYAGAEPICDPLNLFDIAPYADGAAAVL
                     LTTVDHVPTGFTHPLVKVTGSASVIDRLALHDRPDFLRFEAARLSIERACRMAGIMPQ
                     DVDFFELSDTSSIHAALTLEAAGLAPHGQAAQAAAEGRFNLNGALPINTLGGLKARGH
                     PLGATGMYQIVEATLQLRGEAGVNQLGKARRALVQNLGGTAATAVTHVLERVERQNR"
     gene            complement(5034..6107)
                     /locus_tag="PKL11_03035"
     CDS             complement(5034..6107)
                     /locus_tag="PKL11_03035"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNZ12605.1"
                     /translation="MTETQPALLLKPNRPVGIIGYGAYVPRYRLPAAEVARVWTGKSG
                     GGLPVKEKAVAGLDEDVITMSIEAARNALHRAGIAPDALRAVWVGSESHPYAVKPSGT
                     VVAEAIGAAPHVQAGDWQFACKAGTEAMVAAIGLVGSGMGNYALAIGADTAQGRPGDA
                     LEYTAASGAAAYILGPAEEALAVIEATYSFVTDTPDFWRRPYQHYPEHGQRFTGEPAY
                     FKHVYEAAHHLMEATGTQASDYAYAVFHQPNTKFPQRVAGMLGFTPEQIKPGLLSPVI
                     GNTYAAAAIIGLTAILDAAKPGDRILITSFGSGAGSDAMALTVTEAQEERRSRAPLTT
                     DYIARRTQIDYATYVRYRGKLEE"
     gene            complement(6104..7384)
                     /locus_tag="PKL11_03040"
     CDS             complement(6104..7384)
                     /locus_tag="PKL11_03040"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HNZ12606.1"
                     /translation="MSTPKFYDLTPDERRKQIARQVSLTDEEFDALSGCTGLSLEQAD
                     HMIENVIGTYALPLGIAQNFVINGRAVLVPMVVEEPSIVAGASFMARLARSGGGFTTS
                     SDEPLMIGQMQVLNLSDPAGAQQRLLAQKDRLLAAAAAIDPVLERLGGGPRDLEVRLI
                     EESPIGPFVVVHLIYDVRDAMGANAINTATERLTPLVAEISGGRVHLRILSNLADRRL
                     ARARCMVPLTDLAFGDFGAEQVRDGILAAWAFAAADPYRAATHNKGIMNGIDAVVLAT
                     ANDWRAIEAGAHAYAARSGRYTSLSAWSKDEDGNLVGELELPMAVGIVGGATRVHPGA
                     RAALKLMGVKTARDLAEIITAVGLAQNLAALRALATEGIQRGHMTLHARQVAVAAGAT
                     SDEVQRVADRMVQEKAVRLDRAEMILKEIREEKR"
     gene            complement(7541..8230)
                     /locus_tag="PKL11_03045"
     CDS             complement(7541..8230)
                     /locus_tag="PKL11_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNZ12607.1"
                     /translation="MSTQITWYGHATLSVETEGYHLLIDPFFSGNKAAPVSAEQVKAD
                     AILITHGHGDHVGDAIAIAQRTKAVVISNFEICNWLKKNGIANLHGQHIGGGYAHPFG
                     YVKLTPALHGCSLPDGSYGGLAAGFLITTRDGKKLYFAGDTGLFGDMKLIGEGGLDLA
                     ALPIGDNYTMGPADALRAVRMLAPRYVIPIHFNTFELIAQDALAWKTQVEAETTARVQ
                     VLKPGDTFLLD"
     gene            complement(8227..8745)
                     /locus_tag="PKL11_03050"
     CDS             complement(8227..8745)
                     /locus_tag="PKL11_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12608.1"
                     /translation="MKNENRAGLVIGGLLILTGAVFLVSNFVAGYHILRSWPIIFFIL
                     AAGFAVWPFVFPAAKQGLAGLLIPAAILLTLGLIFMYNTLSQDWGAWAYLWILLPGSV
                     GLGLAVAGWIGEWGRATVNVGLWMLGISLLVFAFFSTLFGQMAIRIAGAGILILCGAL
                     LLWRALQKPKTP"
     gene            complement(8801..9376)
                     /locus_tag="PKL11_03055"
     CDS             complement(8801..9376)
                     /locus_tag="PKL11_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12609.1"
                     /translation="MILQPITIAPLQVIRLAELKTVMTGYTTTEKYTVSKVENDFETH
                     LRLALVQLPQPYTRHFDEEISAEDLERYEQVIKEGLSLGCWQGDRLVGVAIAETHHWN
                     RILWIHEFHIAAEERGQGLGRQLMDAMADRARQNGLRALQVETQNTNVPAIRFYREVG
                     YEIEAIDLSYYTNRDVEDYEVAIFMRRKLMA"
     gene            complement(9373..9813)
                     /locus_tag="PKL11_03060"
     CDS             complement(9373..9813)
                     /locus_tag="PKL11_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ12610.1"
                     /translation="MTRAAAIVIRDGMVALIRRVRGGQLYYIFPGGRVEPGETPEEAA
                     IREVTEELGLAIELGPLAAIVTFRGQQQFYFRTYRISGGVFGAGLGPEMLGQYPPENG
                     LYEPVWLPIDQLLTEPVLPRPVAQLVVQAQVSGWSEKVQEYLEG"
     gene            complement(9810..10424)
                     /locus_tag="PKL11_03065"
     CDS             complement(9810..10424)
                     /locus_tag="PKL11_03065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ12611.1"
                     /translation="MKIYFVRHGESEANILKVFSNRPGKHGLTDTGRQQASELAARLR
                     GIRFERIFASPLLRAQQTAEILNQGIGAVIETTDALREYDLGNLEETSYDVGGAAWRE
                     TMDAWLLRQDWDARTGTGENFYEIRGRFEPFVQSLVQYYGGMDVNLLCVGHGGLYRCM
                     LPVVLNNISFEAARNLPIHYASAIIAQKAGQDLMCVQWGDQVML"
     gene            complement(10421..>10854)
                     /locus_tag="PKL11_03070"
     CDS             complement(10421..>10854)
                     /locus_tag="PKL11_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12612.1"
                     /translation="IGRPQIRVPATHRLDPACAPELEAYDFNELTAYNEGYLANWTAE
                     TYQIPVAEAALDARAKFFTEFKEEMRDRIFGSVEDLTVSSADIKVDSFKLLLVPAWLT
                     RYRVDQREYAVIINGQTGQVRANRPASGLKKLVNSIFGVDE"
     assembly_gap    10855..10954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10955..11865)
                     /locus_tag="PKL11_03075"
     CDS             complement(<10955..11865)
                     /locus_tag="PKL11_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12613.1"
                     /translation="MSDGAQDLLTRGIAAAKAGELKEARFFLEWVLTNNPDATQQMEA
                     WLWLSEISTDPAEKRAWLVEALAHDLSEPRARRKLALLDGQLKPADLVNPETIPAIPT
                     GDVTVETQRFTCPKCGGSLTYASDGASLTCEYCESRKSIESSFEVEEGQNFSLAMATV
                     QGHWKPAASKTVQCGGCGANFVLPPEGLAITCPYCASAHVIESVESGEYVVPHALIPF
                     RITAQQAVAALRAWLESNLPEELQWKVLPGTGLYIPVWLFELSGQASWRGYRRNKNNN
                     QVIKDKGEMDIGRPQIRVPATHRLDPA"
     gene            complement(12014..12442)
                     /gene="mscL"
                     /locus_tag="PKL11_03080"
     CDS             complement(12014..12442)
                     /gene="mscL"
                     /locus_tag="PKL11_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNZ12614.1"
                     /translation="MLKEFKEFALKGSLLDLAIGFILGGAFGKIVTSLVNDIIMPPIG
                     LLLGKVNFTDLFWALDGGTYASLQAAKDAGAPTLNYGLFLNTIIDFVIVAFVLFLIIK
                     QVNKLRKPAPAAAPTTKDCPYCKTSIPIGATRCPNCTSEL"
     gene            complement(12460..12864)
                     /locus_tag="PKL11_03085"
     CDS             complement(12460..12864)
                     /locus_tag="PKL11_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNZ12615.1"
                     /translation="MTKLIDIEGIGEAYAVKLKAVGIATVEALLEKGSTTKGRKELAE
                     KTGISDALILKWVNRADLFRIKGIGSEYGDLLEAAGVDTVPELAQRKAENLLPKMTEV
                     NDAKKLVRRMPTLKMVAAWVEEAKKLPRIVNY"
     gene            complement(12961..13950)
                     /locus_tag="PKL11_03090"
     CDS             complement(12961..13950)
                     /locus_tag="PKL11_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNZ12616.1"
                     /translation="MTTLLVILIGLALVFDFLNGFHDSSNLVATIISSRAMSPRMALT
                     LTAIAEFVGPFIFGVAVASTIGEGIVPPHSVTLPVLLAALTSAIFWNLITWFLGIPSS
                     SSHAMIGGLIGAVAAGVGFQNIQLAGLTKVFLALFLSPVLGLLAGYLLTRLVYFLARR
                     ASPKINVFFKRAQIVTAVGLALSHGANDAQKTMGIITLGLVTAGSLAEFKVPIWVVAI
                     SAGAIALGTSVGGWRLIRTLGGKFYKIRPVHGFCTQISSAAVILGAALLGGPVSTTQV
                     VSSAIMGVGSAERLSKVRWNVAGQIAVAWLLTIPATMLVSAGVYYLFRPLMGN"
     gene            complement(13954..14583)
                     /locus_tag="PKL11_03095"
     CDS             complement(13954..14583)
                     /locus_tag="PKL11_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNZ12617.1"
                     /translation="MSLKNIFKPKPDKFLQLLIEQTNLTIKGLEALTQYMHKRNSSLA
                     KQVAETERQADEVRRILVEELTRTFVTPLDREDIFSLSREIDDVLDYAATTVEEMEVL
                     DVSPTPFMERMASLLHDAAVEIDLAVQRLQDKHYTVASDHSQRAKALENRVEQVYREA
                     IADLFQEPKNLKQVMTILKVREIYRHLSNAADREDTAANVISDILMKMS"
     gene            14810..15922
                     /locus_tag="PKL11_03100"
     CDS             14810..15922
                     /locus_tag="PKL11_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12618.1"
                     /translation="MAVIYPFTAIVGQERMRRALVLNAVNPRIGGVLIRGERGTAKST
                     AARALAELLPEVNVVSDCRFGCDPETPNTWCTECRERVARGEALPVATRRTSFVNLPV
                     SATEDRVVGTLDIEKAIQQGERHFDAGILAAANRGLLYIDEVNLLDDHVVDVLLDSAA
                     MGMNIVEREGISFTHPARFILVGTMNPEEGDLRPQLLDRFALSVEIFGIREPRERVII
                     MERNLAFESDAERFRQDWLPKEGELSRQIESARRLLDQVTHTRRDLLSIAALTSSLNV
                     DGHRADLVILKAARAQAAFEGRTAITDRDIALAAELALPHRIRRGPFNQSELSMEQLQ
                     DRIEQLQGSASTGQQREEEGEETDEDTTGQKKKFLT"
     gene            15976..16890
                     /locus_tag="PKL11_03105"
     CDS             15976..16890
                     /locus_tag="PKL11_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ12619.1"
                     /translation="MFDQSTAHWWEGGKKVKPTESFMPRRLDTPLDKMIRRYAGRRSR
                     TRTERKRGRYVQARPSNGNPYDLAFDATLRAAAPFQRRRAEQRQRVAFAVQPGDYMRK
                     VRVRRAANLILFLVDASWSMAVAERMAATKGAILSLLTDAYQRRDRVGLIVFQKDRAT
                     LVLPPTNSVLLAQRALLNIPVGGKTPLAAGLQMATDVLRHEKVFHPDVMPLLLVLTDG
                     AGNVSMGHLPPQEEAYRVAEGIAEDKIHCVVINMEHAAFDQGLAQALAEHLNAPCYTI
                     TELRAESLYLAVKQEMHQRGSVNIHELK"
     gene            16974..17417
                     /locus_tag="PKL11_03110"
     CDS             16974..17417
                     /locus_tag="PKL11_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12620.1"
                     /translation="MKSTADRRTRTRSTLVLIILATLPCYCLGFIVFQVGQALQKTPL
                     PPTATLMAPTETLLGPTAVVTLTSTPLNFPTFTVSPTPTITWTPSITYTPFLPPTRTV
                     TATPTPTLSPTPTNTPLPSHTPTATHTLPPEDTPTPTSTLSSANP"
     gene            17443..18510
                     /locus_tag="PKL11_03115"
     CDS             17443..18510
                     /locus_tag="PKL11_03115"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ12621.1"
                     /translation="MTEITPFLKELLSVSGLSGTEEPVRRLIKDAWTPLVDEVSISKL
                     GSLHALRKGHGPEPRPRLILAAHMDAIGLMATGVVNGLIRFTEVGGVDPRVLPGQFVT
                     VHGRRDLTGVIVKPDDRSLPPRLRDKPFDMEYLYVDTGLLPAEVAELVRVGDTISFAQ
                     PPVELTGDALAGHTLDNRASVAAVTICLQELQPVHHAWDVWAVATVQEEETLGGALTS
                     PFAIRPQLAIAIDVTFARGPGSPESRTYPLGKGITLGFGPNVHPAIYKAFSELADRID
                     LPYSNEYMPRHSGTDAYGMQIVAEGIPTMVIGIPLRYMHTPVEVVSLKDIQRAGHLMA
                     QFISALPADFVNQIRWDEAND"
     gene            18503..19591
                     /locus_tag="PKL11_03120"
     CDS             18503..19591
                     /locus_tag="PKL11_03120"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12622.1"
                     /translation="MTERTKLPIPLPQIGAEQLALLERLSNAVAVSGDEGEVRAIVLE
                     QVKPLADEVTVDALGNVLVTKHATAPNALRVMLAAHMDEVGFMLTRDDENGLYQFGLV
                     GGMDERVLVGKPILVGKSHIPGVIGAKPIHLQERGEREQTIPVEQLRIDIGPGGSGKV
                     KVGDRATFATRFQQIGPSLVGKALDDRLGVAMLLEILRHPIPQLELLLAFTVQEEIGL
                     RGARVAGYALDPQIAIAVDSTPARDLPNYDGTENQSYNTRLGAGPAIYVADSGTLSDP
                     RLIRHLAQIGDALSIPYQFRQPGGGGTDAGAIHTRRAGVPSVSISVPGRYAHTPVLFA
                     RLDDWQNSLSLLHAALQRLTPDILAQER"
     gene            19657..20655
                     /locus_tag="PKL11_03125"
     CDS             19657..20655
                     /locus_tag="PKL11_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNZ12623.1"
                     /translation="MKELIRKLVEAVGPSGYEGAVRDLVRSEISDSVGELRVDAMGNL
                     IARKGQRQNGGLRIMLSAHMDEIGVMATHIDENGFVRFTTIGGVHPRTCFGGRVQFVN
                     GTRGLIGGERTDAPDKIHSFEQLFIDVGASSRETCPVKVGDIAAFDRPMIDLGDRLIS
                     KAMDDRIACAVLIETLRAIKKSPHELFFVFSTQEEVGLRGATTAAYAIDPDLGISVDV
                     TLSGDTPKGVKMEVALGKGPAIKVRDGGMLADPRVVRLMVASAEKAGLPYQLEVLEGG
                     TTDARAIQMARAGVPSGCVSIPCRYVHSPSEMVDYRDVQQSVQLLTALLSGPIALE"
     gene            20670..21986
                     /locus_tag="PKL11_03130"
     CDS             20670..21986
                     /locus_tag="PKL11_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ12624.1"
                     /translation="MPGSPARWLPGGAVLLTLIVFLAGCVNWGPTPAATPSLGAATAS
                     PSPDLTITVTPEPTGSPAANSPITLTLWLPPEFDPYRSEPAAEALLARLQSFVTENPG
                     VRINVRLKAQEGPGGLLEALSAAAPVAPDVMPAVVAMRASDVEAAAHQGLLLPLDELS
                     AAVDDTDWYAYAHALADVDGQAYGLPFTGDVLLMAYRPAQVDTPPQTWQELLLWGRPV
                     LFPAADEHAWLTFLLYQGYGGTLLNDHAIPTLQAAPLAQVLDIYTRGLQQGAFSYNAI
                     QTSSDADVWKAFLEQRSNVVVTWSSNYLQTMPADAMAAPLPALEKRWITLADGWVWCI
                     TDPNPERQALSIRLAEYLTASDFMSDWSQKAGALPARPSALAAWPNQTLRAVLGPIAL
                     SAQEIPGYNIVQTAGPVLQAAVLQVLRQESDPFPAAQSAVDELSAP"
     gene            21998..22351
                     /locus_tag="PKL11_03135"
     CDS             21998..22351
                     /locus_tag="PKL11_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12625.1"
                     /translation="MNRWLRRKTKSIQLPDWLGRNLLLFGIFICFLLGGLFLIGPDLP
                     ATGAAGMQIAITVTPPPVRLDTPVTATPLPAYLTDTSVTDGVIIWGIILVLIVVIGTL
                     GYIRSQTPRPSKPAR"
     gene            22728..23768
                     /gene="pheS"
                     /locus_tag="PKL11_03140"
     CDS             22728..23768
                     /gene="pheS"
                     /locus_tag="PKL11_03140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNZ12626.1"
                     /translation="MDEQGILAELNQIQQDALAALAGIEDETTLQGWKVSYLGRTSPV
                     MQVFSRMGQFSKELRPLVGQQANQVKVALEAALAERSAAVQQAALTRRLSAERLDVTL
                     PGRRIPAGGLHPTTQTLREIYRVLGDMGFQTYRAYDVETDDNNFTLLNIPPHHPAREM
                     WDTYYTTTPGVVLRTHTSPGQIRAMREYAPEPIRVILPGMCYRYEQISHRHEIQFNQV
                     ELLAVGRNITFGDLKGTLNDFARRMFGENVRTRLRPSYFPFTEPSAEMDVECFVCGGK
                     GCQVCKGSGWLEILGCGMVHPTVLENGGYDPREFSGFAAGMGPERITLLRHRIEDIRY
                     FWGNDIRFLEQF"
     gene            23784..26321
                     /gene="pheT"
                     /locus_tag="PKL11_03145"
     CDS             23784..26321
                     /gene="pheT"
                     /locus_tag="PKL11_03145"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNZ12627.1"
                     /translation="MKVVLSWLKDFVDLDLSLEDLARVLTRIGLEVDEVHSIGLPYPG
                     DPGSVSGGYQEFKFTGLSWDRDKIVVAQIDEVMPHPNADRLVLCRLQDGLQEHTVLTG
                     APNLLEYRGRGPLAQPLKVAYAREGARIYDGHQEGYVLTTLKRAKIRGVESYSMVCSE
                     KELGISDEHEGIMLLNPDAPAGLPLVDLLGDAVFEISILPNMIRNACMLGVARELSAA
                     LSKPLRKPHRVVAADGPAIDGQVAIQITDPALNPRFALGLVRGVAARQSPDWVQRRLR
                     LAGMRPINTIVDATNYVMLEVGQPLHAFDYDTLVQRAGGQPPTIITRPAQSGEHLATL
                     DGVERTLEPYTILVTDTAGPLSLAGVMGGQESEVTPDTRTVLLEGASWNLVNVRRTVS
                     GQRLNSEAAYRFARGIHPALAAPAVEYCLDLMAQWCGGQIARGLVDVYPNPLPDAVVE
                     LTIADLQRILGIELSLQTMADLLTPLEFTCQIEGDVLRVKAPAHRLDIGTGLVGKADV
                     IEEIARMHGYDKIPARRMADTLPVQRGNSALEREEALRDLLAGLGLREAVTYRLTSPE
                     REARLLPETAPYVELQNPIAPDRCVMRRSLLASMLEVIERNARLSERLAFFEIGPVFW
                     PIEGQQLPAEPLRLAIALTGRRELPTWDQPHAEMMDFFDLKGLVEQMLEGLHLEGVVF
                     EPVTDDPRFHPGKCALLKINAVPMGILGELHPAIQQHYDLPNAPLLAADLDAAAILAL
                     VNARRDTRPVPAFPPVLEDLAVVVDEDLPADRVAEVIRQAGGKLVTAVRLFDIFRGEQ
                     LGAGKKSLAYSVTYQSYDSTLTDAQTQPVRARIIRRLEQELGAKLRS"
     gene            26323..26946
                     /locus_tag="PKL11_03150"
     CDS             26323..26946
                     /locus_tag="PKL11_03150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ12628.1"
                     /translation="MTNQPLWLDWAQRLQAIAQSGLTYTTNVFDIERFEDLRQIAAEI
                     LAAHTDHDLPCISDLLAGEQGYATPKLDVRGVVFRDDKILLVKERADGGWTLPGGWID
                     VGEPPSLAAEREVWEESGFRVRATRLLALFDRNRHGHPAFIFHIYKLFIACDLLGGEP
                     STSIETDGAEFFAEDALPPLSVARTSTDELSAMFRHHRQPGLPTDFD"
     gene            26985..28406
                     /locus_tag="PKL11_03155"
     CDS             26985..28406
                     /locus_tag="PKL11_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ12629.1"
                     /translation="MKTKSLLAFVLGLILLTGCLTPVGSQPTPAVTSTQSPDLTRPPA
                     KTPTPKVSPTATPAWLVPDSKLNGVTVTFWHPWTGDTAVAVDKLVAEFNRTNEKGITV
                     QSLALGSAGLLSEQIPTALSTGMLPDVVVAPMEDLLTWRSQGAALVDLNPYLNDAEWG
                     LSSELVAEIPPTIWAQEAVDGQRLGLPAQRTTQVLFYNQTWARELGFNAPPTTPAAFK
                     EQVCAAAIALNNDRWADNNGMGGWVWDADAETIWTWLVAFGANPLPASADQPYRFDVP
                     ESVSAFTFLKSMIDEGCAWKSRYPLPYEQFAGRQALIYAGSLTDIAAQQRVQELKKST
                     DTWLVIPFPSPSGQPVAVVSGPAYAIFQTAPAEQLAAWIFVRWLTEPSRQAALAQASG
                     AYPLSSTASQLMSAYADDLPQWETTQDWLNWAQPAPGLASWSTARHILEDAAWQVIQP
                     TITPDQIPAVMQELDAMISQYAQ"
     gene            28451..28651
                     /locus_tag="PKL11_03160"
     CDS             28451..28651
                     /locus_tag="PKL11_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12630.1"
                     /translation="MSDPKTSAQPACVACQRTSTEIPLLTLTYQGTEFYICPEHLPIL
                     IHHPQQLTGRLPGVENLNPHEH"
BASE COUNT         6000 a         9034 c         7352 g         6209 t
ORIGIN      
        1 aagccctgca tgagctcctg gtagaagaag tcaaacacgc caaacagaaa accaaccccc
       61 gcgtagatga tccagcgtct ttttgccggc ttaccttggg agttttttgt ttttgtcatt
      121 gaggccttat atcagagcgc ttttttagac gatttaatct aaagggttat gttacctgct
      181 ggcaggcttg gactctgctt ggaagcaggc aaagcccgaa gccagaaaaa tgctttgaat
      241 cgcgcagaat cccactcgtc gggtgcacgc tgtattggct ggcggttttc gccaagaact
      301 atggtttgcc tagatgagtg cttaccaggc agcttgctta tcattgggat ttgcatgttt
      361 ttcctccgca gaagattcta ctacatgcaa aagccttcct gccttttggc agatgggctt
      421 gagctaatgc cgatgggctt tcttttttat ccagacaaat agtccgacgc ccaaaatcat
      481 tgtacaaacc aggatggcac aaagtgagac cggtggcgta taaccggaaa atgaagcgca
      541 gccctcccaa tcactattga ctgtttggca acaataagga gatgccctca ggctgcaatc
      601 agtcggtgcc tcgtggtcaa ggatggtata acctgccgga caactcccgg tcagtaactc
      661 ccaaccttcg gcagggacac aatacatgca ttcatcacct ggccagatct gagcacactc
      721 ttgggtagtg tggttgacaa catctgcagg cgctgaaggc tctgctgtca caggccttga
      781 cgtatagaaa gacaataaac atagaccaac cagcaaaacc gcgatcttta tcttttttga
      841 catggtttgt cactttcacc gtagtttaga atcagccagc caatggattg cgttactgtg
      901 tgagggcggg cgtgaattct gcttgggcag gtcttctggt aattgaaagg cagtaatgcc
      961 cgcttgattc ttgcaccagt tgcaagagga tggtttgtta tctgccttcc ccataatggg
     1021 tcacggttaa ttttaattga aattggttgg atgaacaacc acaacggctt cacgcttcgc
     1081 cagttcttct aattgctccc ggctggggtg cctgggcaat ccgtgtgccc tctccataat
     1141 caaaccaacc atatgcggat gtctcgcaag tcgcacctcc ataagatctg caaacttgga
     1201 tgggtcggtg atgacttcgg ccataccttg aaaacatttt gtacccacac gaatttcaac
     1261 ctgcggattc gattgaatat tgcgtaccca atctgccttg agtccacgcg ctgcaccaag
     1321 ataataatcc gcaccaatca actcgtattg cagaggagtc acccgttttt tccctgatct
     1381 tctacctgtg gtcgttaaaa ggagaatgat tttgccgata agtggaccaa gcccaatcgc
     1441 atacagcaca cgatggatct tttgaacatt cttcatgccc gcccagttaa atgttgcagg
     1501 tttcattgct ttttcctttc atctgtgtgc aatcattgat tcatttggaa ttctgtaatt
     1561 tggttctttg tttaactgcc taacggtttg tattacctgc gtgtaggcgg gggagacttt
     1621 gcttggaagc ccaaaataac atggggtcat aaaaattgtt gccgtaggta ctgcagcaaa
     1681 gtggtagtcc tccagttggg cggcatagtg atagggttta catccattat gtaccggttc
     1741 ggtcagcgcg ccagtaagtg ccttacatcg tttttattcc atagccccaa catatttgcg
     1801 atctggaagc ccacataatg ctcaaaatga ggaaatctgg accgataata gcaattccaa
     1861 taactcacaa ttctttgctt cagcaagtag acaaaagccg gattctcatt tatgaattta
     1921 tggtaggact gcattggcat acggtttata ttgtgcaata tgacttcaat tatttcaacc
     1981 acgtattcac cgatgggttg aagaacgaat ggacacaccc agatttcatc ctgcgaaaat
     2041 aatttttcca ggtatttctg ccgaataaac ccatcatggt gtcgagtata aatacaagcc
     2101 aaaatcgcaa gctcttgtgc gctcaatcca tataatgatg ccagccttgg ttctggagaa
     2161 tagatacgag atggaatatg tattagttct ccagagatag taattgggcc aacatcatac
     2221 tgtgtaggtt ctaaaaaaca ggttggcaaa atggcaacca cagcctctac atcttttgac
     2281 aggtagcttg gaaacgcggc ctgaagatgt ttaatagtct ctggccgggg agctaacgtt
     2341 tcaaggtttt tcgtcatggc tcattttcac tgcgtctggt agccgcttta tatcattgtt
     2401 tgggcgaagc tcctccatca accggtggat tccccccacc ctcagccggg gcgggtggaa
     2461 ccatagcagc ttgcacagcc gggctcggtg cgggtgctgc tgtaggggcc ttcttgcgcg
     2521 accccagcca gatgccaacc ccaatagcca gcgccagcag cgcgccgccg cagaacaggg
     2581 ccgccacaaa atcaatctca gtggccgtgg tggtattcat tccggtggcc cgtttgatgg
     2641 cattgatcac gtccacaaag aaattatctc tggatggatg aatgatctca aacggcgtta
     2701 atggatcgcc aataaaggga ctgagccgcc aggtcatctg cgcccccaca aacccataca
     2761 gcacaaccca catgcgcagc aaggcattcc ccagcgaact gttcgccatt tcgcgattct
     2821 gatcaaccca cttgaaccca ttgaggacgt aataaagccc ggtaacaccg ctgatggcta
     2881 caaatatcac tgccaccagt tggaagaacg cataattgga agaggtcagc acaaaaaaca
     2941 gcgtcaccgg cgacagcccc agcaacagga atgccgacac gccaatccca ctgaggacaa
     3001 taatccctgc ctgagccacc gaaaagcgca cattcaagag ggccagcgca aagaaataca
     3061 gggctggcag cgtaaatatt agcgtcagca aaaatagcag cggcatcttg atggctgtag
     3121 aaagcccctg cagccaactg tgcgataacc ccgttacaaa gccgtagagc atcaaaaaca
     3181 ccacaattga aaccagcagc cctaaagcct tgcctttcag gttttcgttg cgctgaacgc
     3241 tctggtaaaa cgctttggga tctttgagga aattgggaat cagcataaaa acaggttgtt
     3301 tcataatccc tctcctattt cgttgattgt atgtctgggg aaaagataat aaagcgccct
     3361 tcacattcga agggcgcttt ggatacttac tgcattagcg gtctaaactt atacccataa
     3421 accagaatcc cccggtcgtt gccatcctgc cggatcttgc gggtcaccat ttcaaccggc
     3481 atgccaattt ccaccgcctg gtcgcccagg tcagtcaatt gtgcggttat caccgggcct
     3541 tcttctaact gaaccagcgc cacggtgtag ggcgcctggg cttcgtaacc cgccggggca
     3601 tcctgcatca tcgtgaacga aaacaccttg ccgcgtccgc taaaaccata gggggttttg
     3661 gcttcgccac cgcagtgcgg gcaaacatcc cgcggcggaa aaatcttgcc atcgcagtgc
     3721 gggcaggttt cgcccaccag ggcatatcgc tgtttcttca aacgccagtg acgtggaatt
     3781 tccatttttt ctgcttctcc ttcaactgta tcgtgccaaa gtgtaccggt aacagtctat
     3841 caattcctat caagttaccg attctggcgc tcaactcgtt ccaaaacatg ggtcacggca
     3901 gttgccgccg tcccgcccag gttttgaacc agcgctcgcc gggctttacc aagttgatta
     3961 accccggctt cgccgcggag ttgcagtgtc gcttccacaa tttgatacat ccccgtggcc
     4021 cccagcggat gcccgcgggc cttcagtccg cccagcgtgt tgatcggcag ggcgccgttg
     4081 aggttgaacc gcccctcagc cgccgcctgc gcggcctgcc cgtgcggggc cagtccggcg
     4141 gcttccagcg tcagcgcggc atgaatggac gaggtatcgc tcaattcaaa gaaatccaca
     4201 tcttgcggca taatccctgc catccggcag gcgcgttcaa tcgaaagccg cgccgcctca
     4261 aaccgcagga agtccggacg gtcgtgcagt gccaggcggt caatcaccga ggcggacccc
     4321 gttaccttga ccagcgggtg tgtaaacccg gttggaacat ggtccacggt ggtcagcagc
     4381 accgccgccg cgccatcagc ataaggcgca atatcgaaca aattcaacgg atcacaaatc
     4441 ggctccgccc cggcatacac ctccggcttg atcgttttgc gatacatcgc gttggggttc
     4501 ccggcgccgt tggcgtgcgc cagcagtggg aaggctgcaa acgcctcgcg gggcgggcgg
     4561 tattcataca ggtagcgctg catcagcagc ccggcttgcg ctgtcggggt cagcccttcc
     4621 acggcttccc agtcataatc ggtcatttca gatacagccg cctctaactc cgagccaacc
     4681 atatcggtgt acttttcaac ccccaccacc agcgctgtat ccaccattcc gctggctacc
     4741 gccaggtagc ccatccggaa ggcggctgcg cccgaggcgt cggcggcttc aactgtgaat
     4801 gcctcaatcc cggtcaggcc ggtattatct gccagcagcg cccccaggtt ggactggtgc
     4861 gaaaccgttg aagccagcat attgccgata tagagcgcct gcggcttcaa ttccattccg
     4921 gcttcgtgcc gcgccgccag catggcccgt gctgccagcg tccgcagcga aagttcccaa
     4981 tgctcgccga ctggaatttg cccaatgcct gcaatcacaa catcacgcat gggttactcc
     5041 tccagcttac cgcggtagcg cacataggtg gcgtaatcaa tttgtgtgcg ccgggcaata
     5101 taatccgttg tcagcggcgc ccggctgcgg cgctcttcct gcgcttcggt cacagtcagt
     5161 gccatcgcgt ctgagcctgc ccccgaccca aaggatgtaa tcagaatccg gtcacccggc
     5221 ttggccgcat ccaggatggc ggtcagtcca atgatggctg ccgccgcata agtattgcca
     5281 atcactggcg agagcagccc cggcttgatc tgctccggcg taaagcccag catccccgcc
     5341 acccgctgcg ggaatttggt attgggctga tgaaatactg cataggcgta gtcgctggcc
     5401 tgggtgccgg tggcttccat caggtgatgc gccgcctcat agacgtgctt gaaatatgcc
     5461 ggttcgcccg taaagcgctg cccgtgctcg gggtaatgct ggtaaggccg ccgccagaaa
     5521 tccggcgtgt cggtcacaaa agagtaggtc gcctcaatca ccgccagggc ttcctcggct
     5581 ggtcctaaaa tatatgccgc cgcgcccgag gccgccgtgt attccagcgc gtcaccgggc
     5641 cgcccctggg ccgtatccgc gccaatagcc agggcatagt tgcccatccc cgagcccacc
     5701 agcccaatgg ctgctaccat cgcttcggtg ccagctttgc acgcaaactg ccagtcgcca
     5761 gcctgcacat gcggcgctgc gccaatggcc tcagccacca ccgtcccgct gggtttgacc
     5821 gcatacgggt gggactcaga gcccacccac accgctcgca gcgcatcggg cgcaatgcca
     5881 gcccggtgca gcgcattgcg ggcagcttca atggacatgg taatcacatc ctcatccagc
     5941 ccggcaacgg ccttttcttt aaccggcagt ccgccgccgg atttacctgt ccacacccgc
     6001 gccacctctg ccgcgggcag ccgataacgg gggacatacg ccccgtaccc aataattcca
     6061 accggtcggt tgggttttag aagtaaagca ggttgggtct cagtcatcgt ttttcctcac
     6121 gaatttcttt aagtatcatt tcagcccggt ccagccgtac cgctttttct tgcaccatgc
     6181 ggtctgccac ccgttgaact tcatcagatg ttgctccagc cgccaccgcg acctgccggg
     6241 cgtgcagcgt catatgccca cgctgaatcc cttcagttgc cagggctcgc agcgccgcca
     6301 gattttgcgc caatcccacc gcagtgataa tctctgccag gtcccgcgcg gtcttcaccc
     6361 ccatcagttt cagcgctgcg cgtgcgccgg ggtgcacccg ggttgccccg cccacaatcc
     6421 ccaccgccat tggcagttcc agttcgccca ccaggttgcc gtcttcgtcc ttgctccagg
     6481 cagaaaggct ggtgtaccgc cctgaccgcg ccgcgtaggc atgcgccccg gcttcaatcg
     6541 cccgccaatc gttggctgtt gccagaacca cggcatcaat cccgttcata atgcccttgt
     6601 tatgagtcgc cgcccggtag gggtctgccg cagcaaacgc ccacgccgcc agaatgccat
     6661 cccgcacctg ctcggcgcca aaatcgccaa atgccaggtc ggtcagcggc accatgcagc
     6721 gggctcgcgc cagccgccgg tctgccaggt tggataaaat ccgcaggtgc acccgccctc
     6781 cgctgatttc tgccaccagc ggcgtcaagc gctcggtcgc tgtgttaatg gcgttggcgc
     6841 ccattgcgtc tcgcacatca tagatcaaat gcacaaccac aaaaggccca atcggtgatt
     6901 cttcaatcaa ccgcacttcc agatcgcgcg gtccgccgcc cagacgctcc agcaccgggt
     6961 caatcgccgc ggcggctgcc agcagccggt ctttctgggc cagcaaacgc tgctgcgccc
     7021 ctgccgggtc tgatagattc aggacctgca tctgcccaat catcagaggc tcgtcgctgc
     7081 tggtggtaaa gccgcccccg ctgcgtgcca gccgcgccat gaaagacgcc ccggcaacaa
     7141 tcgagggttc ttccaccacc atcggcacca ataccgcccg cccattgatc acaaagtttt
     7201 gcgcaattcc caggggcaag gcatacgtgc caatcacatt ctcaatcata tggtcagcct
     7261 gttccaggct taacccggtg cagccgctga gcgcatcgaa ttcttcatcg gtcaggctga
     7321 cctgccgggc aatttgcttc cggcgttcat caggcgtcag atcgtaaaat ttaggtgtgc
     7381 tcatgggtcc tcatgatgtt ccaagtgtaa ttttaggttc ccatcaaaac ctatcatcct
     7441 ggcggcaaat atcaggctgc cagccagctt ttagcaaaac ttgccgccca accgcctcca
     7501 gcagccgggc ggctgtcaat catctcaatc agctaaaacc ctaatcgagc aggaatgtgt
     7561 cgccgggttt taagacctgc acgcgggcgg ttgtctcggc ctcaacctgg gttttccatg
     7621 ccagtgcatc ctgagcaatc agctcaaagg tgttgaaatg aattgggatg acatatctgg
     7681 gcgccagcat ccgaacggct cgcagggcat ccgccgggcc catggtgtaa ttatcgccaa
     7741 tcggcagcgc agccagatcc aaaccgcctt cgccaatcag cttcatgtcg ccaaacaatc
     7801 cggtatcgcc ggcaaaatac agctttttcc catcccgtgt ggtgatgaga aacccagccg
     7861 ccagcccgcc gtaactgcca tcgggcagtg agcagccgtg cagcgcgggg gtcagcttga
     7921 catacccgaa ggggtgcgca tagcctccgc caatgtgctg cccgtgcagg ttggcaatgc
     7981 cgttcttttt aagccagttg caaatctcaa aattgctgat caccacggcc ttggtccgct
     8041 gggcaattgc aatagcatcg cccacatggt cgccgtgccc gtgtgtgatt agaatggcat
     8101 ctgccttcac ctgttcagcg ctgactggtg cggccttgtt gccgctgaag aacgggtcaa
     8161 tcagcaggtg gtagccttca gtttccacgc tcagtgtggc gtgtccatac caggtaatct
     8221 gcgtgctcat ggcgttttgg gtttttgcag cgcgcgccac agcagcagcg caccgcacag
     8281 aatcaagatc cccgctccgg caatgcggat ggccatttgc ccaaacaggg tgctgaaaaa
     8341 tgcaaagacc agcaggctga tgcccagcat ccacaacccc acgttgaccg ttgcacggcc
     8401 ccattcaccg atccagccag ctaccgccag ccccaacccc acgctgccgg gcagcaagat
     8461 ccacagataa gcccacgcgc cccagtcttg tgacagggta ttgtacataa aaatgagccc
     8521 cagggtcagc aaaatggcgg ctggtatcaa cagtcctgcc aggccttgct tggcggctgg
     8581 aaatacgaag ggccagacgg cgaaccccgc tgccagaatg aaaaatataa tcggccaact
     8641 ccgcaggata tgatatcccg ctacaaagtt ggataccaga aagacagctc ctgtcaatat
     8701 cagcaagcca ccaatcacca atcccgcccg attttcgttt ttcataacag cctccttgaa
     8761 aaatcaattt acaatgtcat tgtagcgcag attgggccgg ttaagccatc aatttgcgcc
     8821 gcataaaaat tgccacctca taatcttcca catcccggtt cgtgtaatac gaaagatcaa
     8881 tggcttcaat ttcatatccc acttcccggt aaaatcgaat ggcgggaaca ttggtatttt
     8941 gagtttccac ttgcagtgcc cgcagcccgt tctggcgggc tcggtctgcc atggcatcca
     9001 tcaactgccg ccccagcccc tggccgcgct cttctgccgc aatatgaaac tcatgaatcc
     9061 acaggatccg gttccagtga tgggtttcgg caatagccac ccccaccagc cggtctccct
     9121 gccagcagcc cagcgacagg ccctctttga ttacctgctc ataacgttcc aaatcttcag
     9181 ccgagatttc ctcatcaaag tgccgggtgt agggttgggg cagttgcacc aacgccagcc
     9241 gcaggtgggt ttcaaaatca ttctccacct tgctgacggt gtacttttct gtggtggtgt
     9301 aaccggtcat gacagttttc agttctgcaa gccgaatgac ttgcaggggg gcaatggtaa
     9361 tcggttgcaa aatcatccct ctagatattc ttgaactttt tccgaccagc cgctgacctg
     9421 tgcctggacc accagttgtg ccaccgggcg gggcagcact ggctctgtca ataactggtc
     9481 gattggcagc cagacgggtt catacaaacc gttttctggc ggatactgcc ccagcatttc
     9541 ggggcccagt cctgcgccaa aaactccgcc gctgattcga taggtccgaa aataaaattg
     9601 ctgctgtccc cggaatgtca caatggcagc cagcggaccc aactcgatgg ctaacccgag
     9661 ctcttccgtc acctctcgaa tggcagcttc ttcgggcgtt tcaccaggct ccactcgtcc
     9721 ccccggaaag atatagtata gctgcccgcc ccgcacccgc cgaatgagcg ccaccatgcc
     9781 atctcgaatt acaatcgcgg cggcgcgtgt cataacatca cctgatcacc ccattgaacg
     9841 cacatcaaat cctgcccggc tttttgggca ataatggcgc tggcgtaatg gatgggtaaa
     9901 ttgcgcgccg cttcaaagct gatgttgttc agtacaaccg gcagcataca gcgatataac
     9961 ccgccgtgcc ccacacagag caaattcaca tccatccccc cataatactg gaccagtgac
    10021 tgcacaaacg gctcaaatcg gccccgaatc tcgtaaaagt tctcacctgt cccggttcgg
    10081 gcgtcccagt cctgccgcag cagccaggca tccatagttt cacgccaggc cgccccgccg
    10141 acatcatacg aggtttcttc cagattgccc agatcatatt cgcgcagtgc atcggtggtt
    10201 tcgatgacag ccccgatccc ctggttgagg atttcggcag tttgctgagc tcttaacagc
    10261 gggctggcaa aaatgcgctc aaaccgaatg cctctcaatc gggctgccag ttcgctggcc
    10321 tgctgacggc ctgtatcggt caacccatgc ttgccaggcc ggttggaaaa aaccttcaag
    10381 atgttggctt cgctttcgcc gtgccgtaca aaataaattt tcattcatcc accccaaaaa
    10441 ttgaattgac tagctttttc aatccgcttg ccggtcggtt ggcgcgcacc tgccccgtct
    10501 gtccattgat gattacggcg tactcgcgtt gatccactcg atagcgggtc aaccaggccg
    10561 gtaccagcag cagcttgaag gagtctactt taatgtctgc agaactaacg gtcaggtctt
    10621 ccaccgagcc aaaaatccgg tcccgcatct cttccttgaa ttcagtaaaa aatttagcac
    10681 gggcatctaa tgccgcttca gctactggaa tctgataagt ttctgcagtc caatttgcca
    10741 gatacccttc attgtacgcc gtcagttcat tgaaatcata agcttctaat tcgggggcac
    10801 aggcagggtc taatcgatgg gtggctggta cccggatttg tggacgccca atgtnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaggca gggtctaatc gatgggtggc
    10981 tggtacccgg atttgtggac gcccaatgtc catctcgccc ttatccttga tcacttgatt
    11041 attatttttg ttgcgtcggt agccgcgcca gctagcctgc ccgcttaatt caaataacca
    11101 gaccgggata tataagcctg tgccggggag aactttccat tgtagttctt ctggcaggtt
    11161 gctttccaac cacgcgcgca gtgctgcaac agcttgctgg gctgtaatcc gaaaaggaat
    11221 cagcgcatgc ggtacaacat actccccgct ttcaaccgat tcgataacgt gtgccgaagc
    11281 acagtaaggg caggtgatcg ccaatccttc gggcggcagc acaaaattag cgccgcagcc
    11341 gccgcactgc actgttttgc tggctgctgg cttccagtgc ccctgcacag ttgccatcgc
    11401 caatgaaaaa ttttgcccct cttctacttc aaaggatgat tcgatggatt tgcggctttc
    11461 acagtactcg caagttagag aggcaccgtc tgacgcatat gtcaagctgc caccgcactt
    11521 tgggcaggtg aatcgctggg tttccacagt cacatcaccc gttgggattg caggaatggt
    11581 ttcagggttt accagatctg ctggctttaa ttgaccatcc agcagagcca actttcggcg
    11641 ggcgcggggc tctgaaagat catgcgccag cgcctctacc agccaggcgc gcttttcggc
    11701 cgggtcggtg ctgatttcac tcagccagag ccaggcttcc atttgctggg tggcatccgg
    11761 attgttggtc agcacccatt ccaaaaagaa acgcgcttct ttcaactcgc cggctttggc
    11821 agccgcaatc ccgcgggtta agagatcttg tgcaccatca gacatcaggc agcctcaaat
    11881 gaagatgatt attgctatca taatctaaca aaaatattgg atggatgaat gacaaagaat
    11941 gctttgattg tactataaag ccgccctcag gattggtgag ggcggctttg gatttacagc
    12001 gaaaccgtga tgattatagt tcagacgtgc agttcgggca gcgggtggcg ccaatcggaa
    12061 tgctcgtctt gcagtagggg cagtccttgg tcgtgggggc tgccgccgga gcgggtttgc
    12121 gcagtttgtt cacctgtttg atgatcagga acagcacaaa cgccacgatc acgaaatcaa
    12181 tgatcgtatt caagaacagc ccatagttca aggtcggtgc gccagcgtct ttggccgcct
    12241 ggagtgaggc ataggtgccg ccatccagcg cccagaacag gtccgtaaag ttgaccttgc
    12301 ccagcagcag gccaatgggc ggcatgatga tatcgttcac cagcgaggtg acgatcttac
    12361 caaacgcccc gccaaggata aagccaattg ccaggtcgag caggctgccc tttagggcaa
    12421 attccttaaa ctctttgagc attttaaagc tcctgtagat tagtagttaa caatccgggg
    12481 cagtttcttg gcttcttcga cccatgctgc caccatcttg agggtgggca tgcggcggac
    12541 cagtttcttg gcatcgttca cttcggtcat cttgggcagc aggttttcgg ctttgcgctg
    12601 cgccagttcg ggcaccgtat caacaccggc agcttccagc aggtcgccgt attcactgcc
    12661 aatgcccttg atgcggaaca ggtctgcccg gttgacccac ttgaggatca atgcgtcgct
    12721 gatgccggtt ttctcagcca gctctttgcg gcctttggtg gttgaaccct tttcaagcag
    12781 tgcttcaacg gtggcgatac ccacagcctt caactttaca gcataagctt cgccaatgcc
    12841 ttcaatatca atgagttttg tcatcttttc ctccaaaaat taggctattt ttgtgtccca
    12901 gccagatggc ttacttgaag tatagtacag atatccggat tgtcaacaat gggagggctc
    12961 tcaatttccc atcagcggtc taaacaggta ataaacccct gcagaaacca gcatggttgc
    13021 cggaatggtc agcagccagg ccacagcaat ctgcccggca acgttccacc gcaccttgct
    13081 taaccgttca gccgaaccca cccccataat cgccgaactg accacctgcg ttgtgctgac
    13141 cggccccccc agcagcgccg cgccgagaat gaccgccgcc gaactgatct gtgtgcagaa
    13201 gccatgcacc gggcgaattt tgtaaaactt gccgcccagt gtgcggatca ggcgccagcc
    13261 gccaaccgag gtgcccaggg caatcgcccc cgcactgatc gccaccaccc agatcggcac
    13321 cttaaattca gccaggctcc ccgctgtcac cagccccagc gtaataatcc ccatggtttt
    13381 ctgggcatcg ttggcgccat ggctcaaagc caggcccaca gccgtcacaa tttgagcacg
    13441 cttgaaaaaa acgttaatct ttgggctggc ccgccgggcc aaaaagtaca ccagccgggt
    13501 caaaagatag cccgccagca gccccagcac aggcgacaaa aacaaagcca gaaaaacttt
    13561 ggtcagcccg gccaactgaa tattttgaaa gccaaccccc gccgccaccg caccgatcaa
    13621 tcccccaatc atcgcatggg atgaactgct gggaatgccc aaaaaccatg taatcaggtt
    13681 ccagaagatg gcgctggtca gcgccgccag cagcaccggc agggtcacac tgtgcggcgg
    13741 cacaatccct tcaccaatcg tcgaagccac cgcaacaccg aaaataaacg gccccacaaa
    13801 ctcggcaatt gccgtcagcg tcagcgccat ccgcggcgac atcgcccgcg aggagatgat
    13861 ggtagccacc agattgctgg aatcgtgaaa cccgttgaga aaatcaaaaa ccagagccag
    13921 cccaatcagg atgacaagca gcgtggtcat ggcttagctc attttcatta atatgtccga
    13981 gatgacgtta gccgccgtat cttctcgatc tgccgcgttg ctcagatggc gataaatttc
    14041 tcgcaccttc aaaattgtca tcacctgttt gaggttcttg ggctcctgga acaaatccgc
    14101 aatcgcttcg cggtacacct gctcaacccg gttctccagc gctttggcgc gctgtgagtg
    14161 atcactggca accgtgtaat gcttgtcttg caggcgttgc actgccaggt caatttccac
    14221 cgctgcatca tgcagcagcg aagccatgcg ctccataaaa ggggtggggg atacgtccag
    14281 aacttccatt tcttccaccg tggtcgcggc ataatccagc acatcatcaa tctcgcgcga
    14341 gagcgagaaa atatcctccc gatccagcgg cgtgacgaat gtgcgcgtca gctcttccac
    14401 taaaatccgc cgcacctcat ccgcctgccg ctccgtctca gccacctgtt tcgccagaga
    14461 tgagttgcgc ttgtgcatgt actgcgtcag cgcttccaac cccttgatgg tcaggttggt
    14521 ttgttcaatt aaaagctgca ggaacttatc tggtttgggt ttgaagatat ttttcaggct
    14581 catgttgccc atccttgtca tccccggctc ccattaaatt cagaccgcgc cggggtgaaa
    14641 tttaatttta ccagagattt aacaatctgt taacaaagca atcgcaccgt tgaatcacat
    14701 caaatatgcc aataaacata tacaaagcca tcatccgtaa cttgaaagcc ttttcgggtg
    14761 gtggtacact tagagccgca ctaaactttc aagcaaaaga gtacccgcta tggcagtcat
    14821 ttacccattt accgcaattg ttggtcaaga acgcatgcgc cgcgccctgg tcttgaatgc
    14881 cgtcaatcct cgtattggtg gtgtcttgat ccgcggcgag cgtggcacgg caaaatccac
    14941 cgctgcccgc gcccttgcag aactattacc cgaggtcaac gtcgtcagcg attgccgctt
    15001 tggctgcgat cccgaaaccc ccaacacctg gtgtaccgaa tgtcgtgaac gcgtagcccg
    15061 gggtgaagcg ctgcccgtcg ccacccggcg gacctctttc gtcaacctgc cggtctcggc
    15121 aaccgaagac cgtgtggttg gtaccctcga cattgaaaaa gctattcagc agggcgagcg
    15181 ccattttgat gccggtattc tggcggctgc taaccgcggc ttgctgtata tagatgaagt
    15241 caacctgttg gatgatcatg tcgtagacgt gctgctcgat tctgccgcca tgggcatgaa
    15301 tatcgtcgag cgtgaaggca tctccttcac ccacccggcg cgctttatcc tcgtcggcac
    15361 tatgaacccc gaagaaggcg acctgcgccc ccaacttctg gaccgctttg ccctctccgt
    15421 tgaaatcttt ggcattcgcg aaccgcgcga acgcgtgatt attatggaac gcaacctcgc
    15481 ctttgaatcc gatgccgagc gtttccgcca ggattggctg cccaaagagg gcgaactctc
    15541 ccgccagatt gaatctgccc gccgcctgct ggatcaggtc acgcataccc gccgcgacct
    15601 gctctccatt gccgcgctca cctcttccct caatgtggac ggccaccgtg ctgacctcgt
    15661 catcctgaaa gccgctcgcg ctcaggccgc ctttgaaggc cgcaccgcca tcaccgaccg
    15721 ggatattgcc ctggcggctg agctggccct gccacaccgc atccgccgtg gtccgttcaa
    15781 ccagtccgaa ctctcaatgg aacaactgca agatcgcatt gagcagcttc agggcagcgc
    15841 cagcaccggg cagcagcgtg aagaagaagg cgaagagacg gacgaagaca ccaccggaca
    15901 aaaaaaaaag tttctaacgt agagctgggt gaaggcccaa accccgaagg cgagcccgaa
    15961 cccggccccg attcggtgtt tgatcaatcc accgcgcact ggtgggaagg tggcaagaag
    16021 gtaaaaccca ccgaatcctt catgccccgg cggctggata cccctctcga taaaatgatc
    16081 cggcgttatg ccgggcggcg ttcccgcacc cgtaccgagc gcaagcgcgg ccgctatgtg
    16141 caggcgcgtc cttccaacgg caacccctac gacctggcct ttgatgccac ccttcgcgcg
    16201 gccgccccat ttcaacgccg ccgtgccgaa cagcgccagc gggttgcttt tgctgtccag
    16261 cccggcgatt acatgcgcaa ggtgcgcgtt cgccgcgccg ccaacctcat cctcttcctg
    16321 gtagatgcct cctggtccat ggctgttgcc gagcgcatgg ctgccaccaa gggcgccatc
    16381 ctctccctgc tgaccgatgc ctatcagcgc cgcgaccgcg tgggcctgat tgtcttccag
    16441 aaagaccgcg ccaccctggt actgccgccc accaactcgg ttctgctggc gcagcgtgcc
    16501 ctgctcaaca tcccggtggg gggcaaaacc ccgcttgccg ccggcctgca gatggctacc
    16561 gatgttctgc gtcacgaaaa agtttttcac ccagacgtaa tgcccctgct cctggtcttg
    16621 accgatggcg cgggcaatgt ttcaatgggc cacctgcccc cgcaggaaga agcctaccgc
    16681 gtggcagaag gcatcgccga agataagatc cattgcgttg tcatcaatat ggagcacgcc
    16741 gcttttgacc aggggctggc tcaggcgctg gctgaacatc ttaatgctcc ctgctacacc
    16801 attactgaac taagagccga aagcctctat ctggcagtca agcaagagat gcaccagcgc
    16861 ggctcagtta atatccatga gttgaagtag tgatttatgg cccagaatcc cctgcctttt
    16921 atgcttgcca tgacaaccac cccgctttat aatggacagg tggagtctct tcaatgaaat
    16981 cgacggctga ccggcgcacc cgaacccgat caaccctggt gttgattatt ctggcgaccc
    17041 tgccgtgtta ttgcctgggc ttcattgtgt ttcaggtggg gcaggcgctt cagaaaaccc
    17101 ccttgccgcc cacggcaacc ctcatggcgc ccactgaaac gctgctcggc ccaactgccg
    17161 tggtcacctt aacctcaacc ccattgaatt tccctacatt cacagtcagc cccaccccca
    17221 ccatcacctg gacccccagc atcacctaca cacccttcct gccgcccacc cgcacggtga
    17281 cggctacccc caccccaacg ctgagtccaa cccccacaaa tacacctcta ccgagccata
    17341 cccccacagc cacacatacg ttgccgccag aagatacccc caccccaacc tccacactgt
    17401 cttcggcaaa tccataggat gaggagaacc aatagcgatc ccatgacgga aataaccccc
    17461 ttcctgaaag aactgctctc tgtctccggt ctatccggca ctgaagagcc cgtgcgccgc
    17521 ctgattaaag atgcctggac gcccctggtg gacgaagtca gcatcagcaa actgggcagc
    17581 ctgcacgccc tgcgcaaagg gcatggcccc gagccgcgcc cgcgcttaat tttggccgct
    17641 cacatggatg ccatcggcct tatggcgact ggcgttgtga atggcctgat ccgcttcact
    17701 gaagtcggcg gtgttgaccc ccgcgtgctg cccggtcagt ttgtcaccgt tcatggtcgc
    17761 cgtgacctga ccggtgtcat cgtcaagccg gatgaccgct cgctgccccc ccgcctgcgc
    17821 gacaagccct ttgacatgga atacctctat gtcgatacgg gcctgctccc cgccgaggtg
    17881 gccgaactgg ttcgcgttgg tgacactatt tcgtttgccc agccgccagt tgaactgact
    17941 ggcgacgccc ttgccggtca tactcttgat aaccgtgcct ctgtcgcggc agtcactatc
    18001 tgcctccagg agcttcagcc ggttcaccac gcctgggatg tctgggctgt agccaccgtt
    18061 caggaagaag aaaccctggg cggcgcgctc acctcgccgt ttgccatccg cccgcagctc
    18121 gccatcgcca ttgatgtgac ttttgcccgt ggccctggct cgcccgaatc ccgcacttac
    18181 ccgctgggca agggcattac cctgggcttt ggccccaacg tccacccggc tatttacaaa
    18241 gccttcagcg aattggctga tcgcatcgat ttgccctatt ctaatgaata catgcctcgc
    18301 cattctggca ctgatgccta cggtatgcaa attgttgccg agggcatccc caccatggta
    18361 atcggcatcc cgctgcgcta catgcacacc cccgtggaag tcgtctccct caaagatatt
    18421 cagcgcgctg gccatttgat ggcgcagttc atttctgccc tgccagccga tttcgtcaac
    18481 cagattcgct gggatgaagc caatgactga acgaaccaaa ctccccattc ctctgcccca
    18541 gattggcgca gaacaactcg ccctgctgga acggctctcc aatgcggtcg ccgtctctgg
    18601 tgatgaaggc gaagtgcgcg ccatcgtcct tgaacaggtt aaacccctgg cggatgaagt
    18661 aaccgtggat gccctgggca atgtcctggt gaccaaacat gccaccgccc ccaatgccct
    18721 gcgcgtgatg ctggctgccc acatggatga agttggcttt atgctgaccc gtgatgatga
    18781 aaacggcctc tatcaattcg gcctggttgg cggcatggat gagcgcgtcc tggtgggcaa
    18841 acccatcctg gtcggtaaat cccacatccc cggcgtgatt ggcgccaagc ccatccacct
    18901 gcaggaacgc ggcgaacgcg aacaaaccat ccccgttgaa caattacgca ttgatattgg
    18961 ccccggcggc tctggcaagg tcaaagtggg tgaccgcgcc acctttgcca cccgtttcca
    19021 gcagattggc ccgagcctgg tgggcaaagc gcttgatgac cgcctgggtg ttgccatgct
    19081 gctcgaaatc ctgcgccacc ccatccctca gcttgagctt ttgctggcct tcaccgtcca
    19141 ggaagaaatt ggcctgcgcg gcgcccgggt tgccggctat gccctcgatc ctcagatcgc
    19201 cattgccgtc gactccaccc ccgctcgcga cctgcccaac tacgatggca ccgaaaacca
    19261 gtcctacaac acccgtcttg gcgctggccc tgccatttat gtcgccgact cgggcaccct
    19321 ttcagacccg cgtctcatcc ggcacctggc ccagatcggc gatgccctca gcatccccta
    19381 tcagttccgg cagcccggtg gcggcggaac cgatgccggc gccatccaca cccggcgggc
    19441 aggcgtcccc agcgtctcca tctcagtccc cggacgctat gcccacaccc cggtgttgtt
    19501 tgcccggctg gatgactggc agaacagcct gtccctgctg catgcagctt tgcagcgcct
    19561 aacccccgat attctggcgc aggagcgcta aaacccacac ccgcttgggc ggatcggcct
    19621 aatccccgcc cttcagatga aaagactgga gcttcaatga aagaactgat ccgtaaactg
    19681 gtagaagcag tagggccttc aggttatgaa ggcgcagtgc gcgatttagt gcgctctgaa
    19741 atttccgatt ccgttggtga actgcgcgtc gatgccatgg gcaacctcat cgcccgcaaa
    19801 ggccagcgtc agaacggcgg cctgcgcatt atgctctccg cccatatgga tgaaatcggc
    19861 gtcatggcca cacatattga tgaaaacggc tttgtccgtt tcaccaccat tggcggcgtt
    19921 catccccgca cctgctttgg cgggcgtgtg cagtttgtca acggcactcg cggcctcatc
    19981 ggcggcgaac gcaccgatgc ccccgacaaa atccattcct ttgagcagct ttttattgat
    20041 gttggcgcct ccagccgcga aacctgcccc gtcaaagtgg gcgacatcgc tgcttttgac
    20101 cgccccatga ttgacctggg cgaccgcctc atcagcaaag ccatggatga tcgtattgcc
    20161 tgcgccgtgc tgattgaaac cctgcgtgcc attaaaaaat ccccccacga gctgttcttt
    20221 gtcttcagca cccaggaaga agttggcctg cgcggcgcca ccactgccgc ctacgccatt
    20281 gatcccgacc tgggtatctc ggtggatgtg accctcagcg gcgacacccc caagggcgtc
    20341 aaaatggaag ttgctctcgg caaaggccct gccatcaaag tgcgcgacgg cggcatgttg
    20401 gctgacccgc gtgtcgtccg cttgatggtc gcctctgccg aaaaagcggg tctgccctac
    20461 cagttggaag tgctggaagg cggcacaact gatgcccgcg ccattcagat ggcccgtgct
    20521 ggcgtcccgt ctggctgtgt atccattccc tgccgctatg tccacagccc ctcagaaatg
    20581 gtcgattacc gcgatgtgca gcaatccgtc caactgctga cggctctgct cagcggcccc
    20641 attgccctgg aatgagagaa aactcgtcct tgcccggcag tcctgctcgc tggctgccgg
    20701 gtggggcagt tctgctaacc ctgatagttt tcctggctgg ctgtgtcaat tgggggccaa
    20761 caccggcagc cacgccttcg cttggcgcgg ccacagcctc tccatcacca gacctgacca
    20821 tcacagtcac gcctgagccc accggctctc ccgcggccaa tagccccatc acgttgacgc
    20881 tctggctccc gccagaattt gacccctacc ggtctgaacc agccgccgaa gccctcctgg
    20941 cccggctcca atcctttgtg accgaaaacc ccggtgtgcg catcaacgtc cgcctcaagg
    21001 cgcaggaagg ccccggcggt ttgctcgaag ccctgtcggc cgccgccccg gttgcccccg
    21061 atgtgatgcc cgcagttgtt gccatgcgcg cctctgatgt tgaagccgct gcccatcaag
    21121 gcttgctgct gccgctggat gaactttctg ccgcagtgga tgacaccgat tggtatgctt
    21181 acgcccatgc cctggccgat gtcgatgggc aggcctatgg tctgcccttt actggtgacg
    21241 tcttgctgat ggcctatcgc ccggcccagg tggatacacc gccccagacc tggcaggaac
    21301 tgctgctctg ggggcgcccc gtcctcttcc ccgctgccga tgagcatgcc tggctgacct
    21361 ttttgctcta tcaaggctac ggcggcaccc tgctcaatga ccatgccatc ccaacccttc
    21421 aggctgcgcc actggctcag gtgctggata tttacacccg cggtttgcag cagggcgcat
    21481 tttcttataa cgccattcaa acgagcagtg atgccgatgt gtggaaagcg tttttagagc
    21541 agcgctccaa tgtcgtggtc acctggagct caaattatct ccaaaccatg cccgccgatg
    21601 ccatggccgc gccgctgcct gccctcgaaa aacgctggat caccctggca gatggctggg
    21661 tctggtgcat caccgatccc aaccctgaac ggcaggcctt gagcattcgc ctggctgaat
    21721 acctgaccgc cagcgacttt atgtccgact ggagtcagaa ggccggcgcc ttaccggcgc
    21781 gtcccagtgc cctggctgcc tggcccaacc agaccctgcg cgctgttctg gggccgattg
    21841 cactttctgc tcaagaaatt cccggctata atatcgtcca aactgccggc ccggtcttgc
    21901 aggcagctgt tttacaggtc ttgcgccagg aaagcgaccc attccccgcc gcccaatcgg
    21961 ctgtggacga gctttcagcg ccctgatgag gttatggatg aatcgatggc ttagacggaa
    22021 aacgaaatct attcagttgc cagattggct ggggcggaac ctgctgcttt ttggcatctt
    22081 tatttgcttt cttttgggcg ggctttttct aattggccca gacctgcccg ccacaggcgc
    22141 ggctggcatg cagattgcca tcacggttac ccctccgccc gtacggctgg atacgcccgt
    22201 caccgccacg cccttacccg cctatctgac cgacacctcg gtaaccgatg gcgtcatcat
    22261 ctgggggatt atcctggtac tgattgtggt gattggcacc ctcggttata tccgttctca
    22321 gacgccccgc ccatccaaac ccgcccggta gcctcagcgc ccaaattcac cccttgacat
    22381 cccctcggtt ttggcgataa ttgaattaat ctttccaacc attaatggct gtgaaggagg
    22441 aaagtaaaca ggtgaaagcc gtcagggaga ccggggcatg gactgaaacc ccgatccggg
    22501 agacaccggt tgaagttccc tcctgagccg ctccggggaa cgcctatcat gggccagtag
    22561 tctgagccgc atcgccagcg ttacagggcg aaccaatcgc cagggcatat cccgggctgt
    22621 tgggtagagt gggctgccgt gtgcagccaa cctgggtggt accgcgggct tcgcctcccg
    22681 tcccttgtcg gacgggcggt tttaatttaa tcaaggaggt tgcttgtatg gacgaacagg
    22741 gcattcttgc cgaacttaac caaatccagc aagacgcatt ggcggctctg gctggtattg
    22801 aagacgaaac gaccttgcaa ggctggaagg tctcttatct cgggcgcacc tcgcccgtca
    22861 tgcaggtttt tagccgcatg gggcagtttt ccaaagaact gcgcccgctg gtcggtcagc
    22921 aggccaatca ggtcaaagtc gcgctcgaag ctgccctggc agaacgttct gccgccgttc
    22981 agcaggctgc cctcacgcgc cgcctgtctg ccgagcggct ggatgtgacc ctgcccggtc
    23041 gccgtatccc cgctggcgga ctgcacccca ccacacaaac cctgcgcgaa atttatcgtg
    23101 ttctcggcga tatgggcttc caaacctacc gcgcctatga tgttgaaacc gatgataaca
    23161 acttcaccct gctcaacatc cccccccatc acccggcgcg ggaaatgtgg gatacctact
    23221 acaccaccac ccccggtgtg gtactgcgca cccatacgtc cccgggtcaa attcgcgcca
    23281 tgcgtgaata tgcccccgag cccatccgcg tgatcttgcc cggcatgtgc taccgctacg
    23341 agcagatcag ccaccgccac gaaattcagt tcaatcaggt tgagctttta gccgttgggc
    23401 gcaacatcac tttcggtgac ctcaaaggca ccctgaacga tttcgcccgt cgcatgttcg
    23461 gcgaaaacgt ccgcacccgc ctgcgcccct cttacttccc cttcaccgaa cccagcgccg
    23521 aaatggacgt ggaatgtttt gtctgcggcg gcaagggctg ccaggtttgc aaaggctctg
    23581 gctggctcga aattctgggc tgcggcatgg tccatcccac cgtgctggaa aacggcggct
    23641 acgacccgcg cgaattcagc ggtttcgcgg ctggcatggg ccccgagcgc atcacgctcc
    23701 tccgccatcg cattgaggat atccgctact tctggggtaa cgatatccgc ttcctggaac
    23761 agttctagat taggagtttc atcatgaaag tcgttctttc ctggctcaaa gatttcgttg
    23821 acctggatct ctcgctcgaa gacctggcgc gtgtgctgac ccgcatcggc ctcgaggtgg
    23881 atgaggttca ctcaatcggc ttgccctacc ccggcgatcc gggctctgtt tccggcggat
    23941 atcaagaatt caaattcacc ggtctgtcct gggaccgcga caaaattgtt gttgcccaga
    24001 ttgacgaggt gatgccccac cccaatgccg accgcctggt gctctgccgc ctgcaggatg
    24061 gccttcaaga acacaccgtc ctcaccggcg cccccaatct tctggaatac cgcggccgag
    24121 gccccctggc ccagcccttg aaagttgcct atgcccgcga aggtgcgcgc atctacgacg
    24181 ggcatcagga agggtatgtc ctcacaaccc tcaagcgcgc caaaattcgc ggcgttgagt
    24241 cttactctat ggtctgctcc gaaaaagagc tgggcatctc cgatgagcat gaaggcatca
    24301 tgctgctcaa ccccgatgcc ccggctggcc tccccctggt cgatctgctg ggcgatgcag
    24361 tgttcgaaat ttccatcctg cccaacatga tccgcaacgc ctgtatgctg ggtgttgccc
    24421 gcgagctttc tgccgccctg agcaagcccc tccgcaaacc tcatcgcgtg gttgccgctg
    24481 atggccctgc aattgacggt caggttgcca tccagattac tgacccggcc ctcaatccgc
    24541 ggttcgccct gggtctggtt cgcggcgttg ctgcccggca gagccccgac tgggtgcagc
    24601 gtcgtctgcg tcttgcgggc atgcgcccca tcaacaccat tgtggatgcc accaactatg
    24661 tgatgctgga agtcgggcag ccgctgcatg ctttcgatta cgacaccctc gtccaacgcg
    24721 ccggcggtca gccccccacc atcatcaccc gcccggccca atctggcgaa cacctggcga
    24781 ccctggacgg cgttgagcgc accctggagc cttataccat cctggtgacc gacaccgctg
    24841 gcccgctctc gctggctggc gtgatgggtg ggcaggagag tgaagtcacg cccgataccc
    24901 gcactgtctt gctggaaggc gccagttgga acctcgtcaa tgttcgccgc acggtcagcg
    24961 gtcagcgcct caattctgag gctgcctatc gcttcgcccg cggcatccat cctgccctgg
    25021 ctgccccggc agtggaatac tgcctggatc tcatggctca gtggtgcggc ggtcaaatcg
    25081 cccgcggtct ggtagatgtc tatcccaatc ccctgcccga tgcggtggtg gaattgacca
    25141 ttgccgacct gcagcgcatt ctgggtattg aactttccct tcagaccatg gccgacctgc
    25201 tgaccccgct cgaattcacc tgtcagatag agggcgacgt gctgcgggtc aaagccccgg
    25261 cgcaccgcct ggatattggc acaggtctgg tgggcaaggc cgatgtaatt gaggaaatcg
    25321 cccgcatgca cgggtacgat aaaatcccag cccgccgcat ggcagacacc ctgcccgtcc
    25381 agcgcggcaa ctcggcgctt gaacgtgaag aagccctgcg tgatctgttg gctggtctgg
    25441 gcctgcgcga ggctgtcacc taccgcctga cctcccccga gcgtgaggcc cgcctgttac
    25501 cagagacggc tccctatgtc gagcttcaaa accccattgc tcccgatcgc tgtgtgatgc
    25561 gccgcagcct gctcgcctcc atgctggaag taatagagcg caacgcccgc ctgagcgaac
    25621 gcctggcctt tttcgaaatt ggcccggtat tctggcccat tgagggtcaa cagctgcccg
    25681 ccgaacccct gcgcctggcc attgcgctga ctggccggcg tgagctgcca acctgggatc
    25741 aaccccacgc tgagatgatg gacttcttcg acctcaaagg cctggttgag cagatgctgg
    25801 aaggcctgca cctggaaggt gttgtgttcg aacccgtcac cgatgatccc cgcttccatc
    25861 cgggcaaatg cgcactgctg aagatcaatg ccgtgccgat gggcattctg ggtgaactgc
    25921 accccgccat tcaacagcat tatgacctgc ccaatgcccc gctcctggcg gctgacctgg
    25981 atgctgcagc gattctcgcg ctggtcaatg cccgccgcga tacccgcccc gtacctgcct
    26041 tcccgcctgt tctggaagac ctggccgtgg tcgtggatga agacctgcct gctgaccgtg
    26101 tggccgaagt catccgtcag gctggcggca aactggtcac tgccgttcgg ttgttcgata
    26161 tcttccgcgg cgagcagctt ggcgccggca agaagagcct ggcttacagc gtcacatacc
    26221 agtcctacga ctcaaccctg accgatgccc aaacccagcc cgtgcgagcc cgcatcatcc
    26281 gccggcttga gcaggaactt ggcgccaagc tgcggagctg aaatgaccaa ccaacctctc
    26341 tggcttgatt gggcccagcg cctgcaggcc attgcccagt ctggtctgac gtacactacc
    26401 aatgtctttg acattgagcg ctttgaggat ctccgccaga tcgcggcgga gatcctggcc
    26461 gcgcacaccg accacgacct gccctgcatt tccgatctgc tcgccggtga acagggctac
    26521 gctaccccca agctggatgt acgcggggtc gttttccgtg acgacaaaat tctgctggtc
    26581 aaagaacgtg cagatggcgg ctggacgctc cccggtggtt ggattgatgt gggtgagccc
    26641 cccagcctgg ctgccgaacg cgaagtctgg gaagaatcgg gttttcgcgt gcgcgccacc
    26701 cgcctgctgg ccctgtttga tcgcaaccgc cacggccacc cggccttcat ctttcacatc
    26761 tataaactgt tcatcgcctg cgacctgctc ggcggcgagc cgtcaaccag tattgaaacc
    26821 gatggcgctg aattttttgc cgaagatgcc ctgccgccgc tttcggtcgc ccgcacctca
    26881 acagatgagc tgtctgctat gttccggcat catcgccagc ccgggctgcc aacggatttc
    26941 gattaattcg cctgatggcg gtttaccggt taaaataggg ggatatgaaa acaaaatcac
    27001 tccttgcctt tgtcctgggc ttgattctgc tgaccggctg cctcacgccg gtcggcagtc
    27061 agcccacgcc ggcggtcacc tccacccaat ctccggacct cacccgccct ccggcaaaga
    27121 cccccacccc caaagtatca cccaccgcca ccccggcctg gcttgtaccc gattcaaagc
    27181 tcaatggcgt cactgtaacc ttctggcacc cctggacggg tgatacggcg gttgcggtcg
    27241 ataaactggt cgccgaattt aaccgcacga acgagaaggg aatcaccgtt caaagccttg
    27301 ccctgggcag tgcgggcctc ctctcagagc aaatcccaac cgccctgtcc actggcatgc
    27361 tgcccgatgt ggtcgtagcc cccatggaag acctgctgac gtggcggtct cagggagcag
    27421 ccctggtgga cctgaaccct tacctgaacg acgccgaatg gggtctctcc tccgagctgg
    27481 tggccgaaat cccgcccacc atctgggcgc aagaagccgt agatgggcag cgcctgggtc
    27541 tgccggcaca gcgcaccacc caggttcttt tctataacca gacctgggcc cgtgaactgg
    27601 gctttaacgc gccgccgacc accccggctg ccttcaaaga acaggtctgt gccgctgcca
    27661 ttgccctcaa taatgaccgc tgggcagata ataatggcat gggcggctgg gtctgggatg
    27721 cggatgctga aaccatctgg acctggctgg tggcatttgg cgccaacccg ctcccagctt
    27781 ctgccgacca accctaccgt tttgatgtcc cggaatcagt ctccgccttc accttcctta
    27841 aatctatgat agatgaaggc tgcgcctgga agagccgcta cccactgcct tacgagcagt
    27901 tcgccggtcg tcaggctctg atctacgccg gcagcctgac agatatcgcc gctcagcagc
    27961 gcgttcagga gcttaagaaa agcaccgata cctggctggt cattcccttc ccctcaccat
    28021 cggggcagcc tgtggccgtg gtcagcgggc cagcctatgc catcttccaa accgcaccgg
    28081 cagagcagct tgccgcctgg atttttgtcc gctggctgac tgagccatcc cgacaggcgg
    28141 ctctggccca ggcatccgga gcctacccac ttagcagcac ggccagccag ctcatgtcag
    28201 cttatgcaga tgacttgcct cagtgggaaa ccacccagga ttggttgaat tgggcccagc
    28261 ccgctcccgg attagcctcc tggagcactg cccgtcacat ccttgaggat gccgcctggc
    28321 aggtcattca acccaccatc acccccgacc aaatccctgc cgtcatgcag gaactggatg
    28381 ccatgatctc ccagtacgca caataaagat aatgtgataa aatatatcca cttcatacta
    28441 ggagaatccc atgtctgacc ccaaaacctc tgcccaaccc gcctgtgttg cctgccagcg
    28501 caccagcacc gaaatccctc tgctgaccct cacctaccag ggtaccgaat tttacatctg
    28561 ccccgagcac ctgcccatcc tcatccacca cccccagcag ctcacggggc gcctccccgg
    28621 cgttgaaaac ctcaacccgc acgaacatta atcaatcctc tcgctgctca aacaacagcc
    28681 cggactatca cgttc
//
LOCUS       DAOPVS010000017        27693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_1701_length_27693_cov_59.743650, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000017 DAOPVS010000000
VERSION     DAOPVS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27693
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1701_length_27693_cov_59.743650"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..3500
                     /locus_tag="PKL11_03165"
     CDS             39..3500
                     /locus_tag="PKL11_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ12631.1"
                     /translation="MKTTIKSIINNLKQKDARGYQNRLMRYLLLATLASIIVIIGINF
                     YELISGTDANAKSYLISDLAALICVTILWLLDQRGFTRLTGEVYLLLGFAIFFLAPDY
                     HTFNETVLVLVIPVMVAGFVINPISSLYTGGAAILLYVLVYIIKSIEMGPLDYKPEWF
                     SLIVMLFFSIGSWIIARHNKKIIDETKTSRQMFADLVDQLPAFVYVVPLKPGSRATYI
                     SPHIEALTGFTVDEWTDDGERWMSQIHPDDRERVASIFEKSQIEHTPYELEYRMLTKE
                     GRLIWVRDEAHVLLNDQKKPIALQGVLVDISQEKQSDETVRSWRTALENQVVERTREL
                     ADTQGRLKLVLESSTDGYWEWNVTTGDVMISASLAKMLGYQISEIPNSLQYWSQLSHP
                     DDLPGVQQAMTDHLEGRVPFYEVEMRLQRKNGDWLWVRDRGRIVEWDDQGRIKKVVGT
                     HTDIQARKQIESALQRREAVLEAVGFAAARFLSNANWEAEMPAALEQLGKALEVNQAS
                     IVHLPNAPEAFWPFETYIPWPTSIVPERIAELPPEKQAEVMAAMMKYMAPLLEGKTVI
                     MRQSEQPESVQKIMDIFGFASNLQVPIFYRGKFWGAISFTDNNMEREWAAPEIEACRI
                     AALTLEAAIQNEATLKALEESQKNYQTLAENISELISMVDLEGRVIFTTPSSQYLFGV
                     PADAMPNMDGLKFIHPDDLPGVMDTARDHVLGKKDPFTITFRAFQSDGKQFWMEASVR
                     PVIDPESEQVSRLVAVCRDITARRETEEKLAERTEELRQANAELQRADEIKNAFLRNM
                     SHDLKTPLSAILSVAEALQEQTYGPITNRQDDALKAIQISGEHLKRLINDAFDLSRLQ
                     AGMLKLDWQEISLEAVCQESVEIIRQLAEEKGLELSVELEPDLPAVQADPLQLRRILV
                     NLLGNAIKFTPAPGKIGLTVGRQNDLNQLRLTVWDTGIGISEADQAQLFRPFFQVNAA
                     GKRLGGSGLGLPLVKELTKLHDWDLIVESAPGQGSRFTVCIPLYSSSVHGGRVEPQPR
                     QILLVVDNPENTQVLYSRLSSSGYDVNLVRAGPEAVTAAGSEQPDIVLIDTSFPEAKG
                     LSVIQQLRTTPQTTHLPILALPTSSDTADRERCLTAGADAVLARPFHTRDLLELIERA
                     LISANGR"
     gene            complement(3487..5178)
                     /locus_tag="PKL11_03170"
     CDS             complement(3487..5178)
                     /locus_tag="PKL11_03170"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNZ12632.1"
                     /translation="MRTRIALIFFCALLAACTAAPPVALTPASTSPTPTAPLAWQTYS
                     NPQWGLTLSYPSDWQLTEQPGDAQTLPALLLQYPGADLILYYHRTDLSGHYEFPRGAG
                     QLEPVGSASLFNQNIARQVLVNNDQPMAVYYGGGAEISAGPWSLAAALISQGDPLSPV
                     VQAQADEILATIALIPMAATPTPSPLPPTPPPPVPLSAGQDIQMSAGFCFDLDAGRLM
                     SSNAACDFLLRTNPALEANTILFVPVSPARFTAQPAGQAACSPDLPELSTAARILTTA
                     DLPGISLCYQTQSGYVGQMVLRQLNYETLTFDYQTSEALGATPASTGLAVEFLGDVTI
                     PDYTRITPGEAFTKTWQFKNTGSTAWTTQFALVFVSGAQMDAQPVIPLGSEVPPGQMV
                     NLSATLVAPEQPGTYVGYWMLQDAAGQRFGLGQYTNEPFYVIIQSAEPQPDMTATPPL
                     ISGNLRIMDAALTAEPTTYSGACPASVAFTGRITTDGLGTFTYQLEAGSGTPGFTFTL
                     PPSEAVSVITGGMQSLDVSFELTFTDSVSGWARLKVIGPKNTVQTLDIPLRITCR"
     gene            complement(5194..6522)
                     /locus_tag="PKL11_03175"
     CDS             complement(5194..6522)
                     /locus_tag="PKL11_03175"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HNZ12633.1"
                     /translation="MPQAVYHFPRGFLWGAATAAHQVEGGNTNNNWSAWEAQPGHIKQ
                     GRRSGLACDWWGGRWKEDLDRAAETGQNAHRFSVEWSRVQPTPDHWDEHAIDYYREIL
                     RGMVSRGITPLVTLHHFTDPLWLMEQGGWENDETPGRFARFVEKVVEAFKEYATLWTT
                     INEPNVYFYFGYVDGVFPPGKKDLKAAFHVLLNLVRGHTLAYRAIHTIQPEARVGFAL
                     NYRSFVPQTAWSPLDKFQARLRFSAYNESFSNALLTGHVRLPGHAARLRDAIGTQDYF
                     GLNYYTRDLVAFDLRCPRQLFARSSYPPGAALSETGFLANVPAGLFEALRWARRFEVP
                     IIITENGWEDSDDTNRPRYLVEHLHQVWRAVNYTWPIKGYFHWSLVDNFEWERGWTQR
                     FGLWGLDVDTQARIRRPSVDLYAAICRENGISSEMVARYAPQALPLLFPE"
     gene            6897..8123
                     /locus_tag="PKL11_03180"
     CDS             6897..8123
                     /locus_tag="PKL11_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12634.1"
                     /translation="MMLLAGCITPTVIAPTLPPAPTAATTPTLALPEATRTPIPPSPT
                     PVLQLGPFRLVAGAETLLPGLLNGLYLPADGALWLSSDRGVARIFANERQVYLTRFWD
                     QVVGADDSGQIWVMSASGGQISSWDGFTWLHYGAEEGWLPLAKHPLRDRVQPGLFTDS
                     NGRLWLLTAWDVRRFDGKQWVVSTPSTTGLPLPWRKSVTTTLAFTVVPGTDTAWLGGC
                     DWENGKPIGGGGLRYFNGRYWENGGLPDNNICISALTANVEGDVWAGGIGEVWQYSAQ
                     SETWQQQAAPALEDTQMTWSVVLDLPVATNGTVWPRLALCGEAGCDLLAQRYRLADGQ
                     WTALTEISPVEDEHVLFDADGAAWRLTRQAVYALEPGAGNPVAEMKVVTAAAGPDGRL
                     WMVGGIGDGNSIWATP"
     gene            8206..9999
                     /gene="glmS"
                     /locus_tag="PKL11_03185"
     CDS             8206..9999
                     /gene="glmS"
                     /locus_tag="PKL11_03185"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNZ12635.1"
                     /translation="MCGIFGIITDREQQLGPILIDAGLRLSYRGYDSVGCATVHQDGT
                     IDLRKDVGKVVEVADRLGFADMIGSRGILQLRWATFGIPSQVNAQPHLDSDGDLVGAH
                     NGNVVNNVELRQQFIAEGMTVRSANDGESCVHAVERYINRGMEPVEAIRQAYNDLAGD
                     YAFVIGRVGEEKLYALKKGSGLVAGVMEHGTVVSSDLPSILPLTRQIVRIQDGEIAIL
                     SPDGIELRSVADGSRIDRPIEMVTESMEAAQKGGYPHFMLKEIHEQPVVARELLHLLD
                     KNSDVERILAHMAKAENIYLVGCGTSYHACMIASVYFSRLAGRATIPVLAPQFIAQYL
                     PAVGPRDVGIFVSQSGETKDVINAVEAAKPRGMTAFSLVNVIGSTLTRICERTLPLAC
                     GYEISVPATKTFTNQVITFLYLATKLAGEDTAPLAALPDLMQQTIEAVESQVQSVEPL
                     LAGWDDLYCLGYGLTYAIALEGALKLKEITYAHCEGMLSTEFKHGPLSAVSPNYPVLF
                     VAGPEDVPVIVSGINEVTCRGGKAIAIGEEDARLRANADHCIVMPPAPDSALSALLSV
                     LPLQLLSYRLSLARGYDPDFPRNLSKTLTVD"
     gene            10181..11182
                     /locus_tag="PKL11_03190"
     CDS             10181..11182
                     /locus_tag="PKL11_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12636.1"
                     /translation="MLNLVTGATGHIGNVLVRRLLELGQRVRVFILPGEDRSALEGLE
                     IEYAEGNVLDPESLAQAMVGVNMVYHLAGVITILPGKNDFVERVNVQGTRNVLAAARR
                     AGVGRVIYTGSIHAFQRVPHGVLVDEAVPFDAEHAINAYDRSKALASMAVQEAVREGQ
                     DAVIACPTGVIGPHDYRLSEMGALIFDWIKTRTAMIIKGAYDFVDVRDVAEGLILAAA
                     RGRTGETYILSGQQIQVPDILKLVSDALGKPRKSICLPTGLARFFSHFAAFFSRLTHT
                     TPRFTPYSIATLDGNSTISHAKAERELGFNPRPLRQTISDTVAWLLKNRKRFERAKE"
     gene            11184..12017
                     /locus_tag="PKL11_03195"
     CDS             11184..12017
                     /locus_tag="PKL11_03195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ12637.1"
                     /translation="MRPDSTWQGKTVLITGASSGIGEATARRLAAEGLVVLLTARRQE
                     RLEALAQEIQSTGGQAHIYPADLSLAEERQRLFERVNAEQPPVDVLVNNAGLGWYGYT
                     ADMPWQTAQQMLAVNLEAVVQLTLLWLPQMRARRQGRIINIGSVVGSIPSQGVAVYAA
                     TKAFVNAFTTAIYRELAGTAVKVSVVRAGPIRTEFYESAAHRSDGLVIPVERLAISVE
                     RVAEAVWRVLKHPRRRIYVPVWLGITPWVEAAFGWIMDRIGPLLLKHQRARADSSGRR
                     Q"
     gene            complement(11978..12574)
                     /locus_tag="PKL11_03200"
     CDS             complement(11978..12574)
                     /locus_tag="PKL11_03200"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ12638.1"
                     /translation="MNAQLLDSEELLNFFKALADANRLKIVGLLAQKPHTVEELAALL
                     NIKPSTVSHHLARLSEAGLVSARTEGHYYFYSLQLEALERMARQFATREALPRLSEDV
                     DMDAYDRKVLASFTTPEGRIRALPMQEKKFQVLLRYVLKAIEPGKQYTEKQLNEVLAG
                     FYEDTASLRRGMIEYKMMARAADGSAYWRLPDESALAL"
     gene            12768..13934
                     /locus_tag="PKL11_03205"
     CDS             12768..13934
                     /locus_tag="PKL11_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12639.1"
                     /translation="MGKDLQLIDVLRKAFPGILDKEADEMIGSGQVLPFPGNTVICLE
                     GALQTTFYIILEGEVEIRKTINDTEVRVLTHLGPGDFFGEMAIIHGAPRAATVVTTCP
                     TTVLEIPREGFSSMIKRSSSMSMAIVQQISKRLRENDEMAIEDLREKAGQLAEAYQQL
                     AEQDYTRRVFLTTIAHELRTPLMAANGFLQILRSGALQGQAFTAALETVSRNLTEITT
                     LTNDILFLQEMELILPAFEQTDVGGLVASAVEQQREKAERNGVGMTMTIAPGLPRIPA
                     DSKSLERAIVAMIDNGIKFSPDGGAVNVDVGFNEVEVWVRIQDHGVGIPAKYLPRIFE
                     RFFHLDEVAGHLFRGAGLGLSIARQVIEQHKGRVTVESELGRGSTFTIHLRRNQ"
     gene            complement(13991..14413)
                     /locus_tag="PKL11_03210"
     CDS             complement(13991..14413)
                     /locus_tag="PKL11_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNZ12640.1"
                     /translation="MKPQLFALLTAICWGVGGYFEKKGLHLGNLSPTMGITIRTLVAV
                     IILGIASYPQWKTLPQAGPKALLYMIIGGGVVAGAVGMLCFYTAIKGAPLTKVMPIAF
                     TSPLFGALMGIMLGGEPLTLKSVVGMLLTVAGIVVLTL"
     gene            14602..15048
                     /gene="mraZ"
                     /locus_tag="PKL11_03215"
     CDS             14602..15048
                     /gene="mraZ"
                     /locus_tag="PKL11_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNZ12641.1"
                     /translation="MFTSFMGQYEHSIDEKGRMTIPARYRELLVTGAVITLGFDENLW
                     ALRTADYEEIEAKIGRMNPADPNVRQLARLILSHAEFVEPDRLGRILLPQFLRDSGHL
                     TDTAVVVGVGRYFEIWSRELWSAQVARLHDPTITQERLALFDLSHQ"
     gene            15045..15998
                     /gene="rsmH"
                     /locus_tag="PKL11_03220"
     CDS             15045..15998
                     /gene="rsmH"
                     /locus_tag="PKL11_03220"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNZ12642.1"
                     /translation="MSHPTVEDHQPPHVSVLYQEILNVLSPESPGRYVDATLGAGGHA
                     WGILKASTPDGELLGFELDPQALEIASQRLAVFGRRAHLRQASYATLGAELARLGWET
                     VQGVVMDLGVSSMELDTAERGFSFQADGPLDMRFDPTAPLTAADLVNQTPEAELADLI
                     WRYGEEPNSRRIARAIVQARPLETTRQLAQVIEQAHRGPRQRIHPATLTFQALRIAVN
                     DELGALERTLPQAVAALAPGGRLAVIAFHSLEDRLVKQFLRTESTDCLCPPAQPICTC
                     NHRATLRLLTRHAITAADNEITANARARSARLRVAEKLLLA"
     gene            16048..16509
                     /locus_tag="PKL11_03225"
     CDS             16048..16509
                     /locus_tag="PKL11_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12643.1"
                     /translation="MAQNLIQAYRQAPWRTQLQWIGLFLLGLVGVVLVAGLWLSITAQ
                     ATSAGVEIQNLEYDREDIQREIADLKTHYAYLTSAAVMEDRAEGLGYVTADMTQAVYV
                     IVPGYVGRQPEILALPITLEAEESEILKPSYTQSLWDILFRARLWQGSIQP"
     gene            16519..18288
                     /locus_tag="PKL11_03230"
     CDS             16519..18288
                     /locus_tag="PKL11_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNZ12644.1"
                     /translation="MKLPAISRFTIVGAILALMAALIPVQMVRIQTNVEARDKSDWFD
                     QNFGYEERTLTPERGNIYDRWGNLLAGNREVYEIGVALNEVESPETIARDLAAVLGAD
                     YAGVLSAASTKYVKGEIEYIALVDFVDADSVAKLAELQKQYSDLPPARRGQENPSLLG
                     LHWTAHLHRSYPENELGSNVIGFVPYLDRSNNSGLYGVEQYYDTMMAGAPLDVVIAVN
                     PTNVQEIPNVQPGDSLILTIDREIQSKMETVIDQAVTDTGSDSGTIIVMNPETGEILA
                     MATTPRLNINEYWNYGEIFTGTTPFNRAISQTLEPGSTFKVITMASALDSGVITPSNT
                     YYDDGWEEVGGINIHNWDYTIWNDQDMTGCMRHSINTCLVWVAKQMGPTLFYDYLLRF
                     GIGHRTNVDLYGEQTWPLSLPGDPDWYQVNLGTNAYGQGLAVTPIQLITAISAVANDG
                     TMMAPHILRAYIRNGRQYDVEPMPIGSPISAETAHTLNEMLAVSLEEEASKALVEGYR
                     ISGKTGTADIPEGDGWSDYTNASFIGWGPTDDPKFIVYVWLEKPKSDRWGSVLAAPVF
                     QQAVEQLVVLMDLPPDTVRLQLK"
     gene            18303..19718
                     /gene="murF"
                     /locus_tag="PKL11_03235"
     CDS             18303..19718
                     /gene="murF"
                     /locus_tag="PKL11_03235"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNZ12645.1"
                     /translation="MLTLADVLEALTGTRPEAASLIISEAAIDSRLVIPGALFVALPG
                     ERVDGHDYVEEAFRRGAHLALVQKDLSAHLSCVDVRGGKLPESLPEAPFCLWVEDTLK
                     AFQSIASFWRRKLNIRVVGITGSVGKSTTKELIYEVLSQRYHTLKSPGNLNNEIGLPL
                     TLLRLSSGYERAVLEMGFYVPGEISFLCDLALPQVGVITNVGTVHAERAGSQEAIARG
                     KAELVQALPPAPNGVAILNYDDPWVRPMAAQTQAQTLFYGLDPEADLWADEIESQGLK
                     GILFRLHYRQETLYLRAPMIGQHSVHTVLRATAVGLAEGLSWQEIVTGLQQGHTQLRL
                     MAVHTESGALVLDDTYNASPESTMAALNLLNELSGRRIAVLGDMLELGPYEKQGHELV
                     GIRAAQVARLLVAVGNRGKMIAEAARLAGMSSRQIMWVETVPEAITVLQGLLKADDVV
                     LVKGSHGLHMERIVSALEAHA"
     gene            19715..20707
                     /gene="mraY"
                     /locus_tag="PKL11_03240"
     CDS             19715..20707
                     /gene="mraY"
                     /locus_tag="PKL11_03240"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNZ12646.1"
                     /translation="MKEGSLALALSGLSFMMAVIWGGPLLRVLRHFKIGKLIRVEEPG
                     AHMVKMGTPTMGGAMIIVPVALLTVLLNAASLLGLNVLGRSVMLPLLVMGGFGLLGAL
                     DDWEGIRGPRRGSGMRIRTKFGLQILLALGAALALRYVLDVPNMYWFSYQGEIDLGFW
                     YIPIATFVIVGACNAINFTDGLDGLAGMISATCFAAYGVIAILQGQVFLARFCFTLVG
                     AIFGFLWFNVHPAQLFMGDLGALALGATLGVVALMSGQWLLLPLIAVIPVSEALSDVI
                     QIVYFRLTKGKRFFKMAPLHLHFELMGWSEMQIVQRFWLVGLLGAMLGVALAVV"
     gene            20709..22097
                     /gene="murD"
                     /locus_tag="PKL11_03245"
     CDS             20709..22097
                     /gene="murD"
                     /locus_tag="PKL11_03245"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNZ12647.1"
                     /translation="MLINTWSGLHILILGAARQGLALARFLARQGAQVTISDQRSAEQ
                     LKSAQKSLAELPIEWVLGGHPLEALNGIDLLCLSGGVPLTIPIVMEAQRRGIPLSNDS
                     QIFMEAVPCPVVGITGSAGKTTTTTLVGRMAQAGLRSPRRAWVGGNIGLPLVDYLDQI
                     RSDDLVILELSSFQLELMTRSPQVGAILNITPNHLDRHGTMEAYAAAKARLLDFQQPD
                     DWAILNREDAGSWALHAGVRGQLVSFGLKQPEGSQMGSFFADDSLWISDGTRAMELMP
                     AEAVQLRGTHNLVNVLAASAIAWAAGLPLESILAGVEGFMGVPHRLEQVRDWHGALWY
                     NDSIATAPERTMAAIRAFEAPLVLMLGGRDKNLPWGDLADLVHQRVEHVVVFGEAADK
                     ILQALGPVRPGQCTKTITRCQGLEAAVQAAAEVAESGDVVLLSPGGTSYDEFRDFEER
                     GERFREWVKALP"
     gene            22094..23389
                     /locus_tag="PKL11_03250"
     CDS             22094..23389
                     /locus_tag="PKL11_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNZ12648.1"
                     /translation="MNTSFVSNSHSRNRASSSQNMAAETSKPTTQTLRLGIDVPLLLI
                     VACLLAFGLLMVFSAGMRPSILATGKPYHFFVNQIKWAILGLAVATTIIYLDYHLLRR
                     FLLPMIGGTLLLLVLVLLFGDLRLGARRSFFNGSVQPSELAKVAILIYLAFWIKSKED
                     QLNKITFGLLPLLGILGIIAGLIMAQPDISAAATIIVLGVMMFFFANGNLKQLIMVAG
                     ITLLIGLLIVGLNSSLSATAAQRIEDFIRGLINPVAASDHIQRSVDSIVRGGFFGVGI
                     GLGEVKNTGLPVAHTDSIFAVIAEETGLLGSILTILLYIGLMWRGLTIASRAADLEGK
                     LLAGGLTFWIVMEAIINMGVMVNLIPFAGNALPLISAGGSSLVTTLAALGLIMSVARY
                     STRQPQTTEGRAYSAVTDLRWRDSRRRQSRARRGTSGGR"
     gene            23310..24416
                     /gene="murG"
                     /locus_tag="PKL11_03255"
     CDS             23310..24416
                     /gene="murG"
                     /locus_tag="PKL11_03255"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNZ12649.1"
                     /translation="MRLLICAGGTAGGVNPALAVVQAVGADAESMLWIGGEGGMEADL
                     VAREAIPFEAIPAAGVHGVGLRALPGNLWRLVRGTLAARRLVRQFQPDVMLFTGGYVA
                     APVAVASPGIPKLLYVPDIEPGMALRFMARFARCIAVTAESSRTYLPRSARVVVTGYP
                     TRSDLAQWQPPAAREALGLNAELPVVLIFGGSKGARSINRAVLANLPQLLAMTQVIHI
                     SGKLDWAEVEAARQTLTPELASRYHAFPYLYKEMGAALAAADLAVSRAGASTLGEYPL
                     FGLPAILVPYPYAWRYQKVNADELVQAGAAELLPDEELTTQLIPMIEALLNDRTQLSA
                     MQQAMRALARPDAAEAIARLVRDLAGLRGRKATS"
     gene            24413..24955
                     /locus_tag="PKL11_03260"
     CDS             24413..24955
                     /locus_tag="PKL11_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12650.1"
                     /translation="MISLNVIFYMYVILFAFIGGMRGWAKELLVMFSVILAMFLTTVM
                     ERYIPFVRDNLAVNAPATLFWIRAVLLLSLAFFGYQTPNLPKLAGSPRFARERLQDIL
                     LGGFLGAVNGYMVVGTLLAYLHSANYPFPIIIPPNPNDPMGQATINLIALMPPHWLGT
                     TPIIFFAVGIAFLFVIVVFI"
     gene            24955..26331
                     /gene="murC"
                     /locus_tag="PKL11_03265"
     CDS             24955..26331
                     /gene="murC"
                     /locus_tag="PKL11_03265"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNZ12651.1"
                     /translation="MAHIHLIGIGGSGLSAIARLLMERGYTVSGSDKQLSPLTRELAE
                     LGAHIYAGHASENIQGADVVVRSSAVPDDNPEVVAALACGTPVLKRHDFLGELMEEYL
                     GIAVAGTHGKTTTSTMLAWMLTALGQEPSYILGGVSKNLGTNAHAGKGRAFVIEADEY
                     DRMFLGLRPDIIVLTVVEHDHPDCYPTMTDYLDAFTAFVQLLHPGGLLLTGYDDPIAR
                     SFNSQVAADCGSFTYGLDAQADYTATELTQHPKGGYAFQASYRGKPLAQVALQVPGEH
                     NVCNALAALAVAHQLNLDLSAAAEALGEFAGAGRRFDVLGEAQGITVINDYAHHPTEI
                     QATLAAARSRYANRRIWAVWQPHTYSRTQTLLDRFITAFGDADEVLVTDIYAARDYDP
                     SFSAAQVVAQMQHPAAHFTPTLAEATDYLLHHLQSGDVLLVLSAGDADKISRQVLDTL
                     THREVNHV"
     gene            26324..26530
                     /locus_tag="PKL11_03270"
     CDS             26324..26530
                     /locus_tag="PKL11_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12652.1"
                     /translation="MSDPKTQRKYEAKAAGGALQLVPSQGEAAHRSRQRRAELLPGMQ
                     PAEQQDLIKRLIDFISTLETPDPR"
     gene            26527..27459
                     /gene="murB"
                     /locus_tag="PKL11_03275"
     CDS             26527..27459
                     /gene="murB"
                     /locus_tag="PKL11_03275"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNZ12653.1"
                     /translation="MTAPTLLPALRSLFGSRLQENVTLANYTTARVGGPADALLVVNS
                     ATELAEAALRLWEQDLPFHILGSGSNVLISDAGVRGLVILNHAHSVRVDAASNPPTVW
                     AESGANFSGVARQVALRGLSGLEWAAAIPGTVGGAVYGNAGAFGSDVQHDLVLAEILH
                     RKDGKQTWTVEQLDYAYRTSALKRQPGQAIILAARFHLTPSTPEAVQAQMKSLTDRRR
                     QTQPPGASLGSMFKNPQGDYAGRLIETAGLKGTRMGGVEVSTVHANFFVNHAGATAAE
                     IAQLLRHVQQSVLKQSGILLEPEIELLGEWEQAS"
     gene            27459..>27693
                     /locus_tag="PKL11_03280"
     CDS             27459..>27693
                     /locus_tag="PKL11_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HNZ12654.1"
                     /translation="MTGKLRIGVLLGGRSGEHEVSLMSARSVLAVLDPAKYAVTQIGI
                     THQGEWVTGEDVLGALQQGRADTLTRVTLLPQPG"
BASE COUNT         5796 a         7378 c         8718 g         5801 t
ORIGIN      
        1 atgaaaccag cgccactttg tccaaatcca gggtgactat gaaaacaacc atcaaaagca
       61 ttattaataa tctaaagcaa aaggacgcca gaggttacca aaaccggttg atgcgctatt
      121 tgctgctggc aacgctggcg tcaatcattg tcattattgg gattaatttt tatgaattaa
      181 tttctggtac agatgccaat gctaaatctt atttaatatc tgaccttgca gccttaatct
      241 gtgtaaccat tttgtggctc ttagatcaac gcggttttac ccggttgacc ggggaagttt
      301 acctgttatt aggctttgcc attttctttc tggcaccgga ttatcataca tttaatgaaa
      361 ccgtactcgt actggtcatt ccagtcatgg ttgccgggtt tgtgatcaac ccgatcagtt
      421 ctttgtacac agggggggcg gcaattttgc tctacgtcct ggtttatata atcaaatcca
      481 tcgaaatggg gccccttgac tataagcccg agtggttctc actgatagtg atgttatttt
      541 tctcgattgg aagctggatc atcgccaggc acaacaaaaa gatcattgat gaaacaaaaa
      601 catctcgaca aatgtttgcc gacctggtgg atcaactgcc agcatttgtt tatgttgtgc
      661 cgctgaagcc gggcagtcgg gcaacctaca tcagcccaca tattgaagcc ttgacaggct
      721 ttacggtgga tgaatggacc gatgacggtg aacggtggat gagccagatt caccccgacg
      781 accgggaacg agtggcgagt atttttgaaa aaagccagat tgagcatact ccctatgaac
      841 tggagtatcg catgctgacc aaagaggggc ggctgatctg ggtgcgcgat gaagcgcatg
      901 tgctgctgaa tgaccagaaa aagccgatag ccctacaagg ggtgctggtg gacatcagcc
      961 aggaaaagca atctgatgaa acggtgcgct cgtggcggac tgcgctggaa aaccaggttg
     1021 tggaacgaac ccgcgaactg gcagataccc agggtcggtt gaaattggtg cttgagtcga
     1081 gcaccgatgg atactgggaa tggaacgtaa ccaccggcga tgtgatgatc agcgcaagcc
     1141 tggcgaagat gctgggctat cagataagcg aaattccgaa cagcctccag tattggtcgc
     1201 agttgtcaca cccggatgat ttgccaggcg ttcagcaagc catgacggat cacctggagg
     1261 gacgggtgcc cttttatgaa gtggagatgc gcctgcagcg caagaacggt gactggttgt
     1321 gggttcggga ccgagggcgg attgttgaat gggacgacca gggacgaatc aaaaaggttg
     1381 ttggcacgca cacagatatt caggcacgca aacagattga atccgccttg cagcgccgcg
     1441 aagcggtgtt ggaagcggta gggttcgccg cggcgcggtt cttatccaat gccaattggg
     1501 aggccgaaat gccggcggcg ctggagcaat tgggtaaagc gctggaggtg aaccaggcgt
     1561 ctattgtcca ccttccaaac gcgcctgaag ctttctggcc atttgaaaca tacataccct
     1621 ggccaacgtc catcgttcct gaacgaatag ctgaattgcc accagaaaaa caagcagaag
     1681 ttatggcagc aatgatgaaa tatatggctc cactattgga aggaaagact gttatcatgc
     1741 gtcagtcgga gcagcccgaa tctgtacaga agatcatgga tatctttggg tttgcatcca
     1801 atctacaggt gcccattttt taccggggca agttctgggg agccatcagt tttacagata
     1861 acaatatgga gcgggaatgg gctgcgcctg aaattgaggc ctgccggatt gcggcgctga
     1921 cgcttgaagc cgccattcaa aatgaggcga ccctgaaagc gttggaagag agccagaaaa
     1981 attatcagac cctggctgag aacatcagcg aactgatcag catggttgat ctggaagggc
     2041 gggtgatttt caccacaccg tccagccagt atttattcgg cgttcccgcg gatgccatgc
     2101 ccaacatgga tgggttgaaa tttatccatc cggatgattt gcccggtgtt atggataccg
     2161 cccgggatca tgtgctgggc aagaaagatc cgttcacaat caccttccgc gcgtttcaaa
     2221 gcgatggtaa acagttctgg atggaggcct cggtccggcc ggtgattgac cctgaaagcg
     2281 aacaggtcag ccggttggtg gcggtctgcc gagatattac agcccggagg gaaacagaag
     2341 aaaagctggc agaacgcaca gaggaattac gccaggcgaa tgccgagctg cagcgcgctg
     2401 atgagatcaa aaatgcgttt ctgcgtaata tgagtcatga tctgaagacg ccgctcagtg
     2461 cgattttatc tgtggccgag gcactgcaag aacagaccta tgggcccatc acaaatcgtc
     2521 aagatgatgc actgaaagcc attcaaattt caggggaaca tttaaagcgc ctgatcaatg
     2581 acgcctttga tctgtccagg ctgcaggcgg ggatgctgaa gctggactgg caggaaattt
     2641 cgcttgaagc ggtctgccag gagagcgttg aaattatccg gcagttggct gaagaaaagg
     2701 gactggaatt atcggtagaa cttgaacccg acctgccagc agtacaggcg gacccgctgc
     2761 aactgcggcg cattctggtg aacctgctgg gcaatgcgat taaattcacg ccggcgccgg
     2821 ggaaaattgg cctgacggtg gggcggcaaa acgacctgaa tcaactgcgc ctgacggtat
     2881 gggatacggg gattggcata tctgaagcag accaggcgca gctcttcagg ccgttcttcc
     2941 aggtcaatgc ggcgggcaag cggctgggcg gcagcgggct ggggttgccg ttggtgaagg
     3001 aattaaccaa gctgcatgac tgggacctga ttgtggaaag cgcgcccggc cagggcagcc
     3061 gcttcacggt atgcatcccg ttgtactcaa gcagtgttca cggcggacgg gtggagccgc
     3121 agccccgcca gattttgctg gtggtagata acccggaaaa tacgcaggtg ctctattcgc
     3181 ggctgtcttc cagcggctat gatgtaaacc tggtacgggc cggaccagaa gccgtgacag
     3241 cggcaggcag cgagcagcct gatattgtgc tgattgatac aagttttcca gaggccaaag
     3301 ggctgagtgt tattcaacaa ctgcggacaa ccccgcaaac aacccatctg ccaattctgg
     3361 cgctgccgac cagcagcgat accgccgacc gggaacggtg cctgaccgcc ggtgcggatg
     3421 ccgtgctggc gcgcccgttc catacccgcg acctgctgga gctgattgaa agagccttga
     3481 tttcggctaa cggcaggtga tgcggagggg aatatccagc gtctggacgg tatttttggg
     3541 gccaatgacc ttgagccgcg cccagcctga gaccgagtcg gtgaacgtca gctcaaagga
     3601 aacatccagc gactgcatgc cgccggtgat gacgctaacc gcctctgagg gcggcagggt
     3661 gaaggtaaat ccgggtgtgc cgctgccagc ttccagttga taggtgaagg tgcccagacc
     3721 atcggtggta atgcggcctg tgaaggcaac ggatgccggg caggcgccgc tgtaagtagt
     3781 cggctcggcg gtaagggcgg catccatgat gcgcagattg ccgctgatca gcggcggggt
     3841 ggcggtcatg tcgggctgcg gctcggcaga ttggatgatg acataaaaag gctcgtttgt
     3901 atattggccc aggccaaaac gctgtccggc agcatcttgc agcatccagt aacccacgta
     3961 ggttccgggc tgctccggag ctaccagggt tgctgacagg ttgaccattt ggccgggggg
     4021 cacttcgctg cccagcggga taaccggctg agcgtccatc tgggcaccag agacgaatac
     4081 cagggcaaac tgggtggtcc aggcagtgga acccgtgttc ttgaattgcc aggttttggt
     4141 gaaggcctcg ccgggagtga tgcgggtata gtcggggatg gtcacatcac ccagaaattc
     4201 cacagccagc ccggttgaag ccggagtggc gcccagggcc tcgctggtct gataatcaaa
     4261 ggtcagggtt tcgtaattca attggcgcaa gaccatttga ccaacatagc cggactgggt
     4321 ctggtagcac agcgagatac ccggcagatc tgcggtggtg aggatccgcg cggcggtact
     4381 cagttcagga agatcagggg aacaggccgc ctgcccggcc ggctgcgcgg taaagcgcgc
     4441 cggtgatacg gggacaaaca aaatagtatt ggcttccagg gcggggttgg tgcgcagcaa
     4501 aaagtcacag gcggcgttgg aagacatcaa ccggccggcg tcgaggtcaa agcagaagcc
     4561 ggcgctcatc tgaatatcct gaccggcaga aagcggaacc ggcggcggtg gggtgggcgg
     4621 caggggcgac ggcgtgggag tggcggccat ggggatgagg gcaatggtcg ccaggatttc
     4681 atctgcctga gcctgcacca ccgggctaag cggatcaccc tgcgagatga gggctgctgc
     4741 aaggctccag ggcccggcgg agatctcggc gccgccgccg taatagactg ccatgggctg
     4801 gtcgttattc accagcacct ggcgggcaat attttgattg aacaggctgg ccgaacccac
     4861 cggctcaagc tgaccggcgc cgcgcggaaa ttcgtaatga ccggatagat cagtccggtg
     4921 gtaatagagg atcagatctg cgccggggta ctgcagcagc agggcgggga gggtttgcgc
     4981 atcaccgggc tgctcggtca actgccagtc ggaggggtaa ctgagcgtga gcccccactg
     5041 cggattggag taggtctgcc atgccagcgg tgcggtgggg gtgggtgagg ttgaggcagg
     5101 tgtgagggca actggcgggg ctgcggtgca ggccgctaac agcgcgcaga agaaaatgag
     5161 agcaatgcgg gtacgcataa aagatccaat gtgttactcc gggaacagca gcggcagcgc
     5221 ctggggcgca tagcgggcga ccatctcgga ggagatgccg ttctcccggc aaatggcagc
     5281 atagaggtcc acgctggggc gacggatgcg agcctgcgtg tccacatcca ggccccagag
     5341 gccaaagcgc tgcgtccagc cgcgttccca ttcaaaatta tccaccagcg accagtggaa
     5401 atagcccttg atgggccagg tgtaattgac cgcacgccag acctggtgga gatgctcgac
     5461 cagatagcgc gggcggttgg tatcatctga atcttcccag ccgttctcgg tgatgatgat
     5521 gggcacctca aagcgccgcg cccagcgcag ggcttcaaag aggccggcag gcacattggc
     5581 aagaaagccg gtctcgctca gggcggcgcc gggggggtag ctgctgcggg cgaaaagctg
     5641 gcgcgggcag cgcaggtcaa aggccaccag gtcacgggtg tagtaattga ggccaaaata
     5701 gtcctgtgtg ccgatggcat cacgcagacg ggcggcatga ccgggcagac gaacatgccc
     5761 cgtcaacagg gcattggaga aagactcatt gtatgctgaa aagcgcaggc gagcctggaa
     5821 tttatccagg ggagaccagg cggtttgggg cacaaacgag cggtaattga gggcaaaccc
     5881 aacgcgcgct tcgggttgga tggtgtggat ggcgcggtag gccagggtgt ggccgcgcac
     5941 cagattgagc aagacgtgaa aggcggcttt caaatctttc ttgccggggg ggaagacgcc
     6001 atccacatag ccaaagtaaa aataaacatt cggctcgttg atggtggtcc acagggtggc
     6061 atattctttg aaggcctcga ccaccttttc aacaaagcgg gcaaagcgtc cgggggtttc
     6121 gtcattttcc caaccgccct gttccatcag ccagagtgga tcggtgaagt gatgcagggt
     6181 aaccaggggg gtgatacccc ggctgaccat gccgcgcagg atttcgcggt aataatcaat
     6241 ggcatgttca tcccagtggt cgggggtggg ttgaacgcgg ctccattcca ccgagaagcg
     6301 atgggcattc tggcctgttt cggcagcgcg atccaggtct tctttccagc ggccacccca
     6361 ccaatcacag gccaggccag agcggcgccc ctgtttgatg tgccccggct gcgcctccca
     6421 ggcagaccag ttattgttgg tattgccgcc ttcaacctga tgtgctgcgg ttgctgcgcc
     6481 ccagagaaac ccgcgcggga aatgataaac agcctgtggc atgaatggcc tccaatgcgg
     6541 atgaatttag cctgtcaggg ttattctatc gtacaattac ggcatggaca agcaagatat
     6601 gctggcttca aactgccatc aaaaacttga attgttttta cgaaccaaat gctactttac
     6661 gctttttcac ccacggctct gattttatac ccctccccta cccctccccg ctcgaccggg
     6721 gaggggtaaa gaggtaaaaa tggtttttgc caacgctttg cgttggcaaa aaccataaat
     6781 gacaagaaag tgatggtttt caattgacaa gctactttgc aaaaggtgtg cgcaatcgtg
     6841 ctcagttaaa ccgatcgaat cgggggccag gacagcttat cgtggggatg tttttgatga
     6901 tgctgctggc gggctgcatc accccaacag tgattgctcc gaccctgccg cctgcaccca
     6961 cggcggcaac cacgccgacc ctggcgctgc ccgaagccac acgcacgccc atcccacctt
     7021 caccgacgcc ggtgctgcaa ctgggcccgt ttcgattggt ggcaggcgcc gaaacgctgc
     7081 tgcccgggct gttaaacggg ttatacctgc cggcggatgg ggcgctgtgg ttatcttctg
     7141 accgaggtgt ggcgcgcatt tttgcaaacg agcggcaggt ctatctgacg cgcttctggg
     7201 atcaggtggt gggggcagac gactccgggc agatctgggt gatgtcggcc tccggcggac
     7261 agatttcaag ctgggatgga tttacctggc tacactatgg cgctgaagaa ggctggctgc
     7321 ccctggcaaa gcatccgctg cgagaccggg tgcagccggg gctgttcaca gattcgaatg
     7381 ggcggctgtg gctgctgacc gcctgggatg tgcggcggtt tgatggcaag cagtgggtgg
     7441 tttcaacccc atcgaccacg gggctgccgc tgccctggcg caagtcggtg acaaccacgc
     7501 tggcgttcac ggtggtgccc ggcacagaca ccgcctggct gggcgggtgc gactgggaga
     7561 atggcaagcc catcggcggc ggcgggctgc gatacttcaa cgggcgctac tgggaaaacg
     7621 gcgggctgcc cgataataac atctgtattt cggcactgac agccaacgtc gagggagatg
     7681 tctgggcggg ggggattggg gaagtatggc agtattcggc tcaaagtgag acctggcagc
     7741 agcaggcagc gcccgccctt gaagacacgc agatgacctg gagtgtggtg ctggatctgc
     7801 cggtggcgac caatggaacc gtctggccgc ggctggctct gtgcggcgag gcaggttgtg
     7861 acctgctagc tcagcgctac cgcctggcag atggtcaatg gacagcactg accgaaatca
     7921 gcccggtgga ggatgaacac gttttgtttg acgcagatgg ggcagcatgg cggctcaccc
     7981 ggcaggcggt ttacgcgttg gaaccggggg caggcaatcc ggtagctgag atgaaggttg
     8041 tgacggcggc agccggacct gatggtcggc tctggatggt ggggggtatt ggcgatggca
     8101 attccatctg ggcaacgcca taaaacgaga ggctgcagag cggtggttat gcgggtataa
     8161 ttagccgcgt ttgagcaaaa aatctattat agggagataa aaggcgtgtg cggaattttc
     8221 ggcatcatca cagaccgtga acaacaactt ggcccgatcc tgattgatgc aggactgcgg
     8281 ctttcttatc ggggatatga ttcggtaggg tgcgccacag tgcaccagga tggcaccatt
     8341 gatctgcgta aagatgtcgg caaggtggtt gaagtggccg accggctggg ctttgccgat
     8401 atgatcggca gccggggcat tctgcaactg cgctgggcaa cctttggaat accatcgcag
     8461 gtgaatgcgc agccgcacct ggattcggat ggcgacctgg tgggtgcaca taacggcaac
     8521 gtggttaata atgtagaact gcggcagcag tttatcgccg agggcatgac cgtgcgttcg
     8581 gccaatgacg gcgaaagctg cgtgcatgcg gtagagcgtt acatcaaccg ggggatggag
     8641 ccggtggagg ccatccgaca ggcttacaac gacctggcgg gcgactatgc ttttgtgatt
     8701 ggccgggtgg gcgaagagaa gctgtatgcg ctcaaaaaag gctcggggct ggtggcgggt
     8761 gtgatggaac acggcacggt cgtctcctcg gacctgccct ctattttgcc gctgacgcgc
     8821 cagattgtgc gcattcagga cggtgagatt gcgattttat ccccggatgg gattgaactg
     8881 cgctcggtgg ccgatggcag ccggattgat cgccccattg agatggtgac agagagtatg
     8941 gaagctgccc aaaaaggcgg ctacccgcac ttcatgctca aggaaattca cgaacagccc
     9001 gtggttgcca gagaactgct gcacctgctg gacaaaaaca gtgatgtgga gcgcattctg
     9061 gcgcacatgg cgaaagctga gaacatttac ctggtgggct gcggcacaag ctaccacgcc
     9121 tgcatgattg cctccgttta tttcagccgg ctggccgggc gggccaccat cccggtgctg
     9181 gcgccgcaat ttattgccca gtatcttccg gcggtgggac cgcgcgatgt gggtattttt
     9241 gtcagccaga gcggtgaaac taaagatgtc atcaatgcgg tggaagcagc caaaccccgc
     9301 ggcatgacgg ccttcagcct ggtgaatgtg attggctcaa ccctgacgcg catctgcgag
     9361 cgcaccctgc cgctggcctg tggctacgaa atcagcgtgc cggcgaccaa aacttttacc
     9421 aatcaggtga ttaccttcct gtatctggcg accaaactag cgggggaaga taccgccccg
     9481 ctggctgccc tgcctgattt gatgcagcag accattgagg cggttgagag tcaggtgcag
     9541 tcggttgagc cgctgctggc tggctgggat gacctgtact gcctgggcta cggcctgacc
     9601 tatgcgattg ctctggaagg cgcattaaag ctgaaagaaa ttacttatgc ccactgcgag
     9661 ggcatgctct caacggaatt caagcatggg ccgctgtctg ctgtcagccc caattacccg
     9721 gtgctgtttg tagccggacc ggaggacgtg ccggtgattg tcagcggcat caacgaggtg
     9781 acctgccggg gcggcaaagc gattgccatc ggtgaagaag acgcccggct gcgcgccaat
     9841 gcagatcact gcattgtgat gccgcctgct cccgacagcg ccctgagcgc gttgttgagc
     9901 gtgctgccgc tgcaactgct gtcttatcgt ctgagcctgg cgcgcggcta tgacccggat
     9961 ttcccgcgca atttgagcaa gaccctgaca gtagattaat cattgtgctg ataaccgacc
    10021 ggccgttctt tgttttttgc aaagagcggc cggttttgat taagaaactc ttggttaatt
    10081 cacaggttga gatgttgatt tgagggcagg aatgtgggta taataaaaca taccgactgg
    10141 tcggtatgtt ttatcgctat cccaatggag gaaaaacaaa atgctcaacc tggtcaccgg
    10201 agccaccggt cacattggca atgtattggt tcgccgcctg ctggagctgg ggcagcgagt
    10261 gcgcgtattt atcttacccg gcgaagatcg ctcggccctg gaagggctgg agattgaata
    10321 tgccgagggc aatgtgctgg atcccgagtc gctggcccag gcgatggtgg gggtcaatat
    10381 ggtttatcac ctggccgggg tgataaccat tttgccgggc aaaaatgatt ttgtggaacg
    10441 ggtgaatgtg caaggcacgc gcaatgtact ggccgcggca cgccgggctg gcgtggggcg
    10501 ggtgatttat accgggtcca ttcatgcctt tcagcgggtg ccgcacgggg tgctggtgga
    10561 cgaagcggtg ccatttgatg cagaacacgc catcaacgct tatgatcggt ctaaagcgct
    10621 ggcctccatg gcggtgcagg aagctgtacg ggaggggcaa gatgcggtga ttgcctgccc
    10681 caccggcgtg attggaccgc acgattaccg gctgtctgaa atgggggcgc tgatttttga
    10741 ctggattaaa accagaactg ccatgattat taagggcgcc tatgattttg tggatgtgcg
    10801 cgatgtggct gaggggttaa ttctggcggc cgcgcgcggg cgaactggcg agacctatat
    10861 tctatccggc cagcagattc aggtgccgga tattctcaag ctggtgagcg acgcgctggg
    10921 caagccgcgc aaatccatct gtctgcccac ggggttggcg cgattcttct cgcattttgc
    10981 agcttttttc agccgtctga cgcacaccac gccgcgcttc acgccctact ccatcgccac
    11041 gctggatggc aattcaacaa tcagccatgc caaggcagaa cgagaactgg gcttcaatcc
    11101 ccgcccgctg cggcagacga tcagcgacac ggtggcctgg ttactgaaaa accgaaaacg
    11161 gtttgagcgc gccaaagaat aaaatgcgac ctgacagtac atggcagggc aaaacagtac
    11221 tgattaccgg tgcctccagc ggaattggcg aagccacggc gcggcggctg gcagctgaag
    11281 ggctggtggt gctgctgacc gcgcggcggc aggagcggct ggaggcgctg gcgcaggaaa
    11341 ttcaaagcac cggtggacag gcccacattt acccggcgga tttgagcctg gcagaagaac
    11401 ggcagcggtt gtttgagcgc gtcaacgcgg agcagccgcc ggtggatgta ctggtgaaca
    11461 atgccgggct gggctggtac ggctataccg ccgatatgcc ctggcaaacg gcacagcaaa
    11521 tgctggcggt gaacctggag gcggtggtgc aattgacgct gctgtggctg ccgcaaatgc
    11581 gcgcccgccg gcagggacgg attatcaata ttggctcagt ggtaggcagc atccccagcc
    11641 agggtgtggc ggtttacgcg gccaccaagg cttttgtgaa tgcgttcacc actgccattt
    11701 accgggaact ggccggcacc gcggtcaagg tcagcgtggt gcgggccgga cccatccgga
    11761 cagaatttta tgaaagcgcc gcccaccggt cagacgggtt ggtcattccg gtggagcggc
    11821 tggcgatttc agtagagcgg gtggccgagg ccgtctggcg ggtgctgaag catccgcggc
    11881 ggcggatcta tgtgccggtc tggctgggca ttaccccctg ggtggaagcg gcatttggct
    11941 ggattatgga tcggattggg ccgctgctgt tgaagcatca aagagccagg gcagattcat
    12001 ctggcaggcg ccagtaagcc gagccatcgg cagcccgcgc catcatttta tattcgatca
    12061 taccccgccg caggctggcc gtatcttcat aaaaacccgc cagcacctca ttgagttgtt
    12121 tttcagtgta ttgtttgccg ggttcaatcg ctttcagcac atagcgcagc aggacctgga
    12181 atttcttctc ctgcatgggc agcgcccgga tgcgcccctc gggggtggtg aaggaagcca
    12241 gcaccttgcg gtcgtaggcg tccatatcca catcttcaga caagcgaggg agggcctcac
    12301 gagtggcaaa ctgccgggcc atgcgctcaa gagcttcaag ctggagggag tagaagtagt
    12361 aatggccttc ggtacgcgct gaaaccagcc cagcctcgga caggcgcgcc aggtggtgcg
    12421 acacggtgga gggtttgata ttcagcaggg cagccagctc ttcaaccgtg tggggctttt
    12481 gagccagcag accaacaatt ttcaggcggt tggcatcggc cagcgccttg aagaaattaa
    12541 gcagttcttc tgaatccagt aattgagcgt tcatcttgca atatccttca ttatgttttg
    12601 atatctatcg aaatgatttt acaaccatca attgttattg tcaagcaaaa aagggttcat
    12661 tttgtcaggg ttttgtaaac ctattctgct cattttcccg tataattaga gggatgcgga
    12721 tacgaccgct aatttatttt gtctttgtgc atggttgagg tgcgcatatg ggtaaggacc
    12781 tccagttgat tgacgtactt cgcaaagcgt ttccgggtat tctggataaa gaagctgatg
    12841 agatgatcgg ctcaggacaa gtattgccat ttccgggcaa tacggtcatt tgcctggagg
    12901 gtgccctcca gacgaccttc tacattatat tagagggcga ggttgagatt cggaagacca
    12961 tcaacgatac cgaggtgcgc gttctgaccc acctggggcc gggggacttc ttcggcgaaa
    13021 tggcgatcat tcacggcgcg ccgcgggcgg cgacggtggt gaccacctgc cccaccactg
    13081 tgctggagat cccccgcgag gggttctctt cgatgatcaa gcgcagcagc agtatgagca
    13141 tggcaattgt gcagcagatc agcaagcgcc tgcgcgaaaa cgacgagatg gcgattgaag
    13201 acctgcgcga gaaggccggg caactggctg aggcctatca gcagttggcg gagcaggact
    13261 atacccgccg ggtatttctg actacgattg cccacgagct gcgtacccca ctgatggcag
    13321 ccaacggttt tttgcaaatt ctgcgcagcg gggcgctgca ggggcaggcc tttacagcgg
    13381 cgctggagac ggtcagccgc aacctgaccg agatcaccac cctgaccaac gatattttat
    13441 tcctgcaaga aatggaactg attctgccag cgtttgagca aacagatgtc ggcggactgg
    13501 tggcatcggc agtggaacag cagcgtgaaa aggctgagcg caacggggtg ggcatgacga
    13561 tgacgattgc ccccggtctg ccgcgcattc cggcagacag caaaagcctg gagcgcgcga
    13621 ttgtggcaat gattgacaac ggcatcaaat tctcaccgga tggcggggcg gtgaatgtgg
    13681 atgtaggctt taacgaggtt gaagtctggg tgcggattca ggatcatggc gtgggtattc
    13741 cagccaaata tctgccccga atttttgagc ggttcttcca cctggatgag gttgccgggc
    13801 acctgttccg cggcgcgggg ctggggctgt cgattgcccg ccaggtgatt gagcagcaca
    13861 agggtcgggt gactgtggaa agcgaactgg ggcgcggttc aacctttacc atccacctgc
    13921 gccgaaacca ataaaacgcg accgtgagga aaatcagcag ggcagccctt tggaggctgc
    13981 cctgaatttt ttagagcgtc agaacaacga tgcccgccac ggtgagcagc atgccaacca
    14041 ccgatttgag ggtcaggggt tcgccgccca gcatgatgcc catgagcgcg ccgaagagcg
    14101 gggaggtgaa ggcaatcggc atcaccttgg taaggggcgc gcccttgatg gcggtataaa
    14161 agcacaacat gcccacagcg ccggcaacca cgccgccgcc aatgatcata tagagcaggg
    14221 ctttgggacc agcctggggc agcgttttcc actgcgggta gctggcaatt cccaggatga
    14281 ttaccgctac aagggtgcga atggtgatgc ccatggtggg agaaaggttg ccaaggtgga
    14341 ggcccttttt ctcaaagtag ccaccaacgc cccagcagat ggctgtgagc aaggcgaaaa
    14401 gttggggttt catgtggttt gtctcctgat ataagaattg gaatatgagg ttctcagatt
    14461 ggtcttaaaa ttcgcgtgaa aacctcttgc aaaacgccag caattggtat atactgtgga
    14521 tagaagtggt agaaagtggg tgaaagtggt acgaatcgcc gggttacccg gcgtttcgcc
    14581 tatttatccg aggtagaact aatgttcact agtttcatgg gtcagtacga gcacagcata
    14641 gacgaaaaag gacggatgac cattccggcc cgctatcgcg agctgctggt cactggcgca
    14701 gtgatcaccc tggggtttga tgaaaacctg tgggcgctgc gcactgccga ctacgaagag
    14761 attgaggcca agattggccg catgaatccg gctgacccta acgtgcgcca gctagcacgg
    14821 ttaattctat cacacgctga atttgttgaa ccggaccggc tggggcgaat cctgctgccg
    14881 caattcctcc gtgactcggg gcatttgacc gatacagcgg tggtggttgg ggtcggcaga
    14941 tattttgaaa tctggtcccg ggaattatgg agcgcgcagg ttgccaggct gcatgacccg
    15001 acgatcaccc aggaacggct ggcgcttttt gacctctcgc atcaatgagt caccccactg
    15061 ttgaagacca ccaacccccg cacgtctctg tactttacca ggaaatactc aatgttttaa
    15121 gcccggaaag ccccggaagg tatgtggacg ccacattagg cgccggaggg cacgcctggg
    15181 ggatcctaaa ggccagtacg ccggatggcg aattactcgg ttttgaactg gatcctcagg
    15241 cgctggagat tgcaagccag cgcctggccg tttttggcag gcgggctcat ttacggcagg
    15301 cttcttatgc cacactgggc gcagaactgg cccggttggg ctgggagact gtgcagggtg
    15361 tggtgatgga cctgggggta tcttcaatgg aactggatac cgccgaacga gggttctctt
    15421 ttcaagccga tggcccgctg gatatgcgct ttgaccccac cgccccactc accgcagccg
    15481 acctggtgaa ccagacgccg gaagccgaac tggctgacct gatctggcga tatggcgaag
    15541 agccgaactc acggcgaatt gcccgagcca ttgtacaggc ccggccgctg gaaaccaccc
    15601 ggcagctggc gcaggtgatt gaacaggccc accgtgggcc ccgccaacgc attcacccgg
    15661 ccacactcac ctttcaggct ttgcggatcg ccgtcaatga cgagttgggc gcgctggagc
    15721 gaaccctgcc acaggccgtg gcggcgctgg cgcctggcgg tcgtctggcg gtcatcgcct
    15781 tccattcact ggaagaccgg ctggtcaagc agtttttgcg aaccgaaagc acagactgcc
    15841 tctgcccgcc ggcacagccg atctgcacct gcaatcaccg ggctaccctg cggctactca
    15901 cacgtcatgc catcaccgcc gccgataacg agataaccgc caatgcccgg gcacgcagcg
    15961 cccgcctgcg ggtggctgaa aagctgctgc tggcataaga cttgcaattt ccagattgaa
    16021 acgatctcac acttgaagaa ggactctatg gcacaaaatc tcatccaggc atatcggcag
    16081 gccccctggc gcacccagct ccagtggatt ggcctgtttt tgcttgggct ggttggggtt
    16141 gtgctggtcg ccgggctctg gttgagcatt actgcccagg ccacctctgc cggggttgag
    16201 attcagaatc tggaatacga ccgtgaagat attcagcgcg aaattgccga cctgaagacc
    16261 cattacgctt acctgacttc ggcggctgtg atggaagacc gcgccgaagg gctgggatat
    16321 gttaccgccg atatgacgca ggccgtttat gtgattgtgc ctggatatgt tggtcggcag
    16381 cccgaaattc tggcgctgcc cattacgctg gaagccgaag aaagcgaaat cctcaagccc
    16441 agttataccc agtcgctctg ggatattctg ttccgcgccc gcctgtggca gggaagcatc
    16501 caaccctaag gaggccaaat gaaactaccc gcaatctctc gtttcacgat tgttggcgcc
    16561 attttggccc tgatggcagc tttgattcct gttcagatgg ttcggattca gaccaatgtg
    16621 gaagcaagag acaaatctga ttggtttgat caaaattttg gctatgaaga acgcaccctg
    16681 acgcccgagc gcggcaatat ttatgaccgc tggggcaacc tgctggcggg caaccgggaa
    16741 gtatatgaaa ttggggttgc gctgaatgag gttgaaagcc ctgaaacgat tgcccgtgat
    16801 ctggcggctg tgctgggcgc agattatgcc ggtgtgttga gcgccgccag tacaaaatat
    16861 gtaaagggcg agattgaata tattgccctg gttgattttg tggatgcgga ttcggtcgcc
    16921 aagctggcgg aactccaaaa gcagtacagc gacctgccgc cggcccggcg cggccaagaa
    16981 aacccaagcc tgctcggatt gcactggacg gcccacctgc accgcagcta ccccgaaaac
    17041 gaattaggat ccaacgttat cgggtttgtg ccatatctcg accgcagcaa caacagcggg
    17101 ctttacgggg tggaacagta ctacgacacc atgatggctg gcgcgccgct ggatgtggtc
    17161 attgcagtta acccgaccaa tgtacaggaa attccaaatg tgcagcccgg cgacagcctg
    17221 attttgacga tagaccgcga gattcagtcc aagatggaaa cggtcattga ccaggcagtg
    17281 accgatacgg gatctgattc agggaccatc attgtaatga accccgagac gggtgaaatt
    17341 ctggctatgg cgaccacgcc ccggctgaat atcaatgagt actggaacta tggtgagatc
    17401 ttcactggaa cgaccccgtt taaccgggcc atcagccaga cacttgaacc cggctcgact
    17461 ttcaaagtga tcaccatggc ctcggcgctg gacagcggcg tgattacgcc gagcaatact
    17521 tattatgatg atggctggga agaagtaggc ggcatcaata ttcacaactg ggactatacc
    17581 atctggaacg atcaggatat gaccggttgt atgcggcact cgattaacac ctgcctggta
    17641 tgggtagcca agcaaatggg acccacgctg ttctacgact acctgctgcg gttcggaatt
    17701 gggcatcgca ccaatgttga cctgtacggc gagcaaacct ggcccttgag cctgccgggt
    17761 gacccggact ggtaccaggt gaacctgggc accaatgctt acggacaagg tttggcggtc
    17821 acccccattc agttgatcac ggcgatttct gctgtggcca atgatgggac gatgatggcg
    17881 ccgcatattc tgcgagcgta tatccgcaat gggcggcagt acgatgttga gcccatgccg
    17941 attggctcac ccatttcggc agaaacagcg cacacactga acgaaatgct ggcggtttcg
    18001 ctggaagaag aggcctcgaa agcgctggtg gaaggctacc gcatctctgg caagaccggc
    18061 accgccgata ttccggaagg cgatggctgg tcggattata ccaatgcatc gtttattggc
    18121 tgggggccga cggatgaccc caagtttatt gtgtacgtct ggctggaaaa gcccaagagt
    18181 gaccgttggg gctcagtact ggcagccccg gtatttcagc aggctgtgga gcagttggtt
    18241 gtgctcatgg acctgccccc cgacaccgtc cgattgcagt tgaaatagaa ggtgaaagct
    18301 ccgtgctaac gctggcagat gttttagaag ccttgacagg cacccgcccc gaagcagcct
    18361 cgctgattat cagtgaggca gcaatcgatt ctcggctggt gattccgggg gcgctgtttg
    18421 tggcgctgcc cggtgaacgc gtggatgggc atgactacgt tgaagaggcc ttccggcgcg
    18481 gggcgcatct ggcactggtt caaaaagacc tgtcggccca ccttagctgc gtggatgtgc
    18541 gcgggggcaa gctgcccgag agcctgccgg aagcaccgtt ttgcctgtgg gttgaagata
    18601 cgcttaaagc cttccaatcc attgcgagtt tctggcggcg caagctgaat attcgcgtgg
    18661 tggggattac cggcagtgtg ggcaaatcga ccaccaaaga attgatttac gaagtgctca
    18721 gccagcggta ccatacgttg aagagcccgg gcaacttgaa caacgaaatt ggcctgccgc
    18781 tgaccctgct gcgcctgagc agcgggtacg agcgcgccgt gctggaaatg ggcttttatg
    18841 tccccggaga aatcagcttc ctgtgcgacc tggcgctgcc gcaggtgggt gtgatcacga
    18901 atgtgggcac agttcatgct gagcgggcag gatcgcagga agcaattgcg cgcgggaaag
    18961 ccgaactggt gcaggccctg ccccccgccc ccaatggggt ggccattttg aattacgatg
    19021 atccgtgggt gcgcccgatg gctgctcaaa cacaggcgca gacgctgttt tacgggctgg
    19081 accccgaagc tgacctgtgg gctgatgaga ttgaaagcca ggggctgaag gggatccttt
    19141 tccggctgca ttaccgccag gaaaccctgt acctgcgggc gccgatgatt ggtcagcatt
    19201 cggtgcatac cgtgctgcgg gcaaccgctg tgggtctggc tgaggggctg agctggcagg
    19261 agatcgtcac cgggctgcag caggggcaca cccaactgcg gctgatggct gtgcataccg
    19321 aaagcggcgc gctggtgctg gacgatacct ataacgcctc gcccgaatcc accatggcgg
    19381 cgctcaacct gctcaatgag ctttcaggtc ggcggattgc cgtgctgggc gacatgctgg
    19441 aactgggacc ctatgaaaag cagggacacg aactggtcgg catacgggca gcgcaagtgg
    19501 cccggctgct ggttgcggtt ggaaaccgcg ggaaaatgat tgctgaggca gcccgcctgg
    19561 cgggcatgtc atcccgacag attatgtggg tcgagacggt gcctgaggcc atcacagtgc
    19621 tgcaaggctt gctgaaggcc gacgacgttg tcctggtcaa aggatctcac gggctgcaca
    19681 tggaacggat tgtttcagca ctggaggcgc acgcatgaaa gaaggatcgc tggcattagc
    19741 cctgagcggg ctgagtttca tgatggcggt catctggggc gggccgctgc tgcgggtgct
    19801 gcgccacttt aagatcggca agctcattcg ggtcgaagaa ccgggcgcac acatggtcaa
    19861 aatgggcacc cccaccatgg gcggggcaat gattattgtg cccgtggccc tgctgacggt
    19921 tctgctcaat gcggcctcgc tgctgggctt gaatgtgctg gggcgctcgg tgatgctgcc
    19981 gctgctggta atgggcggct ttggtctact gggcgcgctg gatgattggg aaggtattcg
    20041 cggcccccgc cgtggttcgg ggatgcgcat ccgcaccaaa tttggcctgc agatcctgct
    20101 ggccctgggt gcggcgctgg cgctgcgata cgttctggat gtgcccaata tgtactggtt
    20161 cagctatcag ggcgaaattg acctgggctt ctggtacatt ccgattgcca cttttgtgat
    20221 tgtgggcgcc tgcaacgcca tcaacttcac cgatggtttg gatgggctgg caggcatgat
    20281 ttcggcgacc tgctttgcgg cttacggtgt gattgccatt ctgcaggggc aggttttcct
    20341 ggcgcggttc tgcttcacgc tggtgggtgc catcttcggg ttcctgtggt tcaatgtgca
    20401 cccggctcag ctctttatgg gcgatctggg agcgctggcg ctgggcgcca cactgggtgt
    20461 ggtggcactg atgagtgggc agtggctgct gctgccattg attgccgtca tcccggtcag
    20521 cgaagccctg agcgatgtca ttcaaatcgt ttatttcaga ctcaccaaag gcaagcggtt
    20581 ctttaaaatg gctccgctgc acctacactt tgagctgatg ggctggagcg agatgcaaat
    20641 cgtccagcgg ttctggttgg tgggtttgct gggagccatg ctgggtgtgg ctctggcggt
    20701 ggtgtaacat gttgattaat acctggtctg gtcttcacat cctcattctg ggagccgccc
    20761 gacaagggct ggcgctggcg cgtttcctgg cccggcaagg tgctcaggtg accatcagcg
    20821 atcagcgcag cgccgagcag ttgaagagcg cccaaaagtc tctggcagaa ctgccaattg
    20881 agtgggtttt gggcgggcat ccgcttgagg cgctgaacgg gattgacctg ctgtgcctgt
    20941 ctggcggtgt gccgctgacc atcccgattg tgatggaagc ccagcgccgc gggattccgc
    21001 tgtcgaacga ctcgcaaatc tttatggaag ccgtcccctg cccggtggtg ggtatcaccg
    21061 ggtcagcggg aaagaccacc accaccacgc tggttgggcg gatggcccag gcggggctgc
    21121 gctcgccgcg gcgggcgtgg gtgggcggta atattggcct gccgctggtg gattacctgg
    21181 accagattcg ctcggatgac ctggtgattt tggagctgtc gagcttccaa ctggaattga
    21241 tgactcgctc gccgcaggtt ggtgcgattt tgaacattac ccccaatcat ctcgaccggc
    21301 acggcaccat ggaagcctat gctgcggcca aggcgcggct gctcgatttt caacagccgg
    21361 acgattgggc cattctcaac cgggaagatg ccggatcgtg ggcgctgcac gccggtgtgc
    21421 gcgggcagtt ggtcagtttt gggctcaagc agcctgaagg cagccagatg ggcagcttct
    21481 ttgcagacga cagcctgtgg atttcggacg gtacgcgggc gatggaattg atgcccgccg
    21541 aggccgttca attacgcggg acacataacc tggtcaatgt gctggctgcc agtgccattg
    21601 cctgggctgc cggactgcca ctggaaagca ttctggcggg cgtggaaggc tttatgggcg
    21661 tgccgcaccg cctggaacaa gtgcgcgatt ggcatggtgc gctgtggtac aacgactcta
    21721 ttgccacagc gccagaacgc acgatggctg ccatccgggc atttgaggcg ccgctggttt
    21781 tgatgctggg cgggcgcgac aagaacctgc cttggggcga cctggctgat ctggtgcatc
    21841 aacgagtgga gcatgtagtg gtgttcggcg aagccgccga caagattttg caagccctgg
    21901 gccccgtgcg gcctggacag tgcacaaaga ccatcacccg ctgccagggg ttggaagctg
    21961 ccgttcaggc agccgccgag gtggcggaaa gcggcgatgt ggtgctgctc tcaccgggcg
    22021 gtaccagcta tgatgaattt cgggatttcg aagagcgcgg cgagcgcttt cgagaatggg
    22081 tgaaggcttt accatgaaca caagttttgt ctccaactca cattcccgca atcgggcttc
    22141 ctccagccaa aatatggctg ctgaaacgtc caagccgacc acgcaaactc tgcggctggg
    22201 cattgacgtg ccgctgctgt tgattgtggc ctgcctgctg gcctttggtc tgctgatggt
    22261 tttttcggcc gggatgcggc cctccattct ggcgacaggc aagccgtatc acttcttcgt
    22321 caaccagatc aaatgggcaa tcctgggatt ggcagtggct accaccatca tttacctgga
    22381 ttaccaccta ttgcggcggt tcttgctgcc catgattggc ggcaccttgc tgctgctggt
    22441 gctggtgctg ttgttcggcg atttgcgcct aggagcgcgg cgttccttct ttaatggctc
    22501 ggtacagcca tcagagctgg cgaaagttgc cattctcatt tatctggcat tctggattaa
    22561 atccaaagag gaccagttaa acaagattac ctttgggctg ctgcccctgc tgggcattct
    22621 gggcattatt gccggtttga ttatggcgca gccagacatc agtgccgcgg caacgattat
    22681 tgtgctgggt gtgatgatgt tcttctttgc caacggcaat ctcaagcagc tcatcatggt
    22741 ggccgggatt accttattga ttggactgtt gattgtgggc ctgaatagct cactttcagc
    22801 cacggctgct caacgaattg aagattttat ccgtggtttg atcaacccgg ttgcagcttc
    22861 tgaccacatt cagcgcagcg tggactcaat tgtgcgcggc gggttcttcg gtgtgggcat
    22921 tggactgggt gaagtgaaga acacgggtct gccggtggcg cacaccgact cgatttttgc
    22981 ggtgattgct gaagaaaccg gcctgctcgg gtccatcttg accattctgc tctacatcgg
    23041 cctgatgtgg cgcggtctga cgattgccag ccgggcagcc gacctggaag gcaagctgct
    23101 ggccggcggg ttgacattct ggattgtgat ggaagccatc atcaatatgg gtgtgatggt
    23161 caacctgatc ccatttgcgg gcaatgccct gccgttgatc agtgctggcg ggtccagcct
    23221 ggtgacgacc ctggcagccc tgggattgat tatgagcgtg gcgcggtact cgacgcgcca
    23281 accccaaaca acagaaggga gagcttatag tgcggttact gatttgcgct ggcgggacag
    23341 ccggcggcgt caatcccgcg ctcgccgtgg tacaagcggt gggcgctgac gccgaatcaa
    23401 tgctgtggat tggcggcgaa ggcggcatgg aagcggacct ggtagcccgc gaagccatcc
    23461 cctttgaggc cattccggct gccggagtgc acggggtggg cctgcgggcc ctgccgggca
    23521 atctctggcg gctggtgcgc ggcacactgg cagcccgccg tctggtacgg cagttccaac
    23581 cggacgtgat gctattcacc ggcggctatg tggccgcgcc ggttgccgtt gccagcccgg
    23641 gcattcccaa gctgctctat gtaccggata ttgaacctgg tatggcgctg cgcttcatgg
    23701 cgcggtttgc ccgctgtatt gctgtgaccg ctgaaagctc tcgcacttac ctgccccgct
    23761 cagcccgtgt ggttgttacg gggtacccca cccgcagtga cctggcccag tggcagccgc
    23821 cggctgcccg cgaggccctg ggattgaatg cggaactacc ggtggtgttg atttttggcg
    23881 gcagcaaagg cgcccgctcg atcaatcgag ctgtgctggc gaacctgccc caactgctgg
    23941 cgatgaccca ggttattcac atcagcggca agctggactg ggctgaggtt gaagccgccc
    24001 ggcaaaccct gacgccggag cttgccagcc gctaccatgc gttcccctat ttgtacaagg
    24061 aaatgggggc ggcgctggcc gccgccgacc tggctgtctc tcgggcggga gcatccaccc
    24121 tgggtgagta tccgctcttt ggcctgcccg ccattttagt gccttatccg tatgcctggc
    24181 gctatcaaaa ggtgaatgcg gatgaactgg tacaggcagg cgcagcagaa ctgctgcccg
    24241 atgaagaact gaccacacaa ctgattccaa tgattgaggc cctgttgaat gaccgcactc
    24301 aattgagcgc catgcagcag gccatgcggg cgctggctcg cccggatgcg gccgaggcca
    24361 ttgcccgact ggtgcgggac ctggctggtc tgcgaggaag gaaggcgacc tcatgattag
    24421 tttgaacgtt attttctaca tgtatgtgat cctgttcgcc tttattggcg gcatgcgcgg
    24481 ctgggccaaa gaactgctgg tgatgttcag cgtcatcctg gcgatgttcc tgaccactgt
    24541 gatggaacgc tatatcccgt ttgtgcgcga taacctggca gtgaatgccc cggcgaccct
    24601 gttctggatt cgggcggtgc tgctgctgtc actggccttc ttcggctatc agacgcccaa
    24661 tctgcccaag ctggcagggt caccgcggtt tgcccgcgaa cgcctgcagg atattctgct
    24721 gggcgggttc ctgggcgcgg tgaacggcta catggttgtc ggcacactgc tggcctacct
    24781 gcattcggcc aattatccct tcccaatcat catccccccc aacccgaatg atccgatggg
    24841 acaggcgacg attaacttga ttgcgttgat gcccccgcac tggctgggca ccacgccgat
    24901 catcttcttt gcggttggga ttgcatttct gttcgtgatt gtggtgttta tctaatggct
    24961 cacatccacc tcatcggcat tggcggcagc ggcctatcgg ccatagcccg gctcctgatg
    25021 gagcggggct ataccgtcag cgggtcagac aaacagctct ccccgctgac gcgtgaactg
    25081 gctgaactgg gcgcgcacat ttatgccggg catgcgtctg agaatattca aggcgccgat
    25141 gtggtggtgc gatcttcggc tgtgccggat gataatcctg aagtggttgc ggcgctggcc
    25201 tgcggcacgc cggtgctcaa acggcatgat ttcctgggcg agctgatgga agaatacctg
    25261 gggattgccg tcgccggaac gcacggcaag acgaccacct cgaccatgct ggcctggatg
    25321 ctgactgctc tgggtcagga gcccagctat attctggggg gcgtctctaa aaacctgggg
    25381 accaatgccc acgcgggcaa gggccgggca ttcgtcatcg aggcggatga atatgatcgg
    25441 atgttcctgg ggctgcgccc ggacatcatc gttttgacgg tggtggaaca cgatcacccg
    25501 gactgttacc ccacaatgac cgattacctg gatgctttta cggcctttgt gcaactgctg
    25561 catcccggcg gcttgctgct gacgggttat gatgatccaa ttgcccgctc attcaacagc
    25621 caggtggcgg cagactgcgg cagcttcact tacgggctag acgcgcaggc ggattacacg
    25681 gcaactgagc tgacccagca ccccaagggc gggtatgcct tccaggcatc ttatcgggga
    25741 aaaccgctgg cgcaggtggc actgcaggtt ccaggtgagc acaatgtctg caatgcactg
    25801 gcggcactgg cagtggcgca tcaattgaac ctggatttga gcgcagccgc cgaggcactg
    25861 ggtgagtttg ctggagccgg gcggcgcttc gatgtgctgg gcgaggcgca gggtatcacg
    25921 gtcatcaacg actatgcgca tcacccgaca gaaatccagg cgaccctggc cgcagcccgc
    25981 agccgctatg ccaaccggcg catctgggct gtctggcagc cgcacaccta ctcgcgcact
    26041 caaacactgc tggaccgctt tatcacggca tttggcgatg ccgatgaggt gcttgtcacc
    26101 gatatctacg ccgcccgcga ttatgaccct tccttttctg ccgctcaggt tgtggcgcaa
    26161 atgcagcatc cggctgccca ttttacgccc accctggccg aggcgacaga ttatttgctc
    26221 catcatcttc agtctggcga tgtgctgctg gtgctttcgg cgggtgatgc cgacaagatc
    26281 agccggcagg tgctggacac cctgactcat cgggaggtta atcatgtctg atcccaaaac
    26341 ccaacgtaag tacgaagcca aagccgccgg gggggcgtta caactcgtcc ccagccaggg
    26401 cgaggcggcg caccgctctc gacagcgccg cgccgaactg ctgccgggca tgcagcccgc
    26461 tgaacagcag gatctgatca aacggctgat tgatttcatc agcacactgg agacacccga
    26521 cccacgatga ccgcaccgac gctgctcccc gcactgcgca gcctgtttgg cagccgcctg
    26581 caggaaaacg tgaccctcgc caattacacc acggcgcggg taggtggacc tgctgacgcg
    26641 ctgctggtgg ttaactctgc caccgagctg gcagaggctg cgctgcgcct gtgggagcag
    26701 gacctgccct tccacattct ggggagtggg tcaaacgtgc tgatcagcga tgcaggagtg
    26761 cgcgggctgg tgatcctcaa ccatgcgcac agcgtgcggg tggatgccgc cagcaatccg
    26821 ccgacagtct gggcggaatc gggcgccaac ttcagcggcg tggcccggca ggtggccctg
    26881 cgcggactga gcgggctgga atgggcagcc gccattccgg ggaccgtggg tggggccgtg
    26941 tacggcaacg ccggggcctt tggctcagac gtgcagcacg acctggtgct ggcagaaatc
    27001 ttgcaccgta aggatggcaa acagacctgg acggtggaac aactggatta tgcgtaccgc
    27061 accagtgccc tcaaacggca gcccggacag gccatcatcc tggcggcacg cttccatctg
    27121 acgcccagca cgcccgaagc cgtgcaggcg cagatgaaaa gcctgaccga tcgccgccgc
    27181 cagacacagc cccccggtgc gagcctgggt tcgatgttca agaatcccca gggcgattac
    27241 gccggacgac tgattgagac cgctggactg aagggcaccc gcatgggcgg ggtggaagtc
    27301 agcacggtac acgccaattt ctttgtcaac catgccgggg caactgctgc cgaaattgcg
    27361 cagcttttgc gccatgtgca gcaaagtgtt ttgaaacagt ctggaatttt actggaaccg
    27421 gagattgaat tgttaggcga atgggagcag gcaagctgat gacaggcaaa ttacgcatcg
    27481 gggtactgct gggtggacgc tccggcgagc atgaagtctc gctgatgtcg gcgcgttcgg
    27541 tgctggcagt gctggacccc gccaagtatg cggtgacgca gattggcatc acccaccagg
    27601 gtgagtgggt caccggtgag gatgtgttgg gcgcgctgca acagggccgg gccgacacat
    27661 tgacgcgcgt cacgctgctg ccccaacccg gcg
//
LOCUS       DAOPVS010000018        24009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2081_length_24009_cov_60.798322, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000018 DAOPVS010000000
VERSION     DAOPVS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24009
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2081_length_24009_cov_60.798322"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..366
                     /locus_tag="PKL11_03285"
     CDS             52..366
                     /locus_tag="PKL11_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12655.1"
                     /translation="MNVALPAFLKVFFWDCDFSALTFSEQPDFIIRRLLQAGNWEAIS
                     WLRTELGDAALRQWIEQHNGAGLQPRQLRLWEVVLDLPHARVSRWVRAAASQPWEGRL
                     AR"
     gene            363..1004
                     /locus_tag="PKL11_03290"
     CDS             363..1004
                     /locus_tag="PKL11_03290"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ12656.1"
                     /translation="MNFTNAGINREQESVLAQIGGWLTEQGYYLAGGTALAIYYGHRQ
                     SVDLDWFTTAHIPDVLALAQRLRETGMPFETGHFAPGTLYGQIGNVRISLIEFCYPLL
                     NPLEVWPANQTALASLDDLVCMKLSAIAQRGTRKDFYDTHLLLGRHCSLADALSLFQK
                     KFNVSDISPVLYGLAYFDDAEKEPDPILLEKITWKQVKKAILQWLKEYSGAVD"
     gene            1059..1418
                     /locus_tag="PKL11_03295"
     CDS             1059..1418
                     /locus_tag="PKL11_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12657.1"
                     /translation="MSTSTKPGALYRAMHNLFLLTAVFGLIMGLINLLGLVSGFNPTY
                     LADGLFNLAFAAANFIGYRLMASRSRTVIWLQVGIIIAGLLYGPLVGRGLALQYPIIG
                     LIYLFILITLRRRGEIQ"
     gene            1485..2432
                     /gene="lipA"
                     /locus_tag="PKL11_03300"
     CDS             1485..2432
                     /gene="lipA"
                     /locus_tag="PKL11_03300"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNZ12658.1"
                     /translation="MTPEPTRARVNPETVDTTPLRRPSWIKVRAPSGETYEQLQALMR
                     RKALHTVCEEAACPNMGECWGSGTATFLMLGNVCTRTCGFCDIQHGRPPALDWLEAER
                     VAQAVASMELQHAVITSVNRDDRRDGGAPIFAMVIRRIRQLRPGCSVEVLIPDFKGSA
                     EALRMVVEARPEILNHNVETVPRLFKTVQPQDHYEWARATLTNAKRMDPDILTKSGIM
                     VGLGETLDEVKAVMDDLRTWGVQILTIGQYLQPSRKHLPMARYYTLEEFAELKEYGLG
                     IGFSWVESAPLVRSSYHAAEQVRALSTIHKRLYGVGQGG"
     gene            2432..3805
                     /locus_tag="PKL11_03305"
     CDS             2432..3805
                     /locus_tag="PKL11_03305"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNZ12659.1"
                     /translation="MAAVLWWLRRDLRLADNPALQRALSFGAGVVPVFILDPHLLNQP
                     APLRQAFLFDGLRSLDADLRARGSRLILRQGQAEVELARLLAETGATRIIAEEDYSPY
                     ARARDAAVAAQLPLEQVLGLTVFHPAHVLKADGQPYRVFTPFSRAWKALPFPGAPSPA
                     PAALPPVPPDLTSLPIPSVSPSTAFPAGEAEAHRRLTDFAAGPLSDYADARNRLDLDG
                     TSALSPYLRLGMLSAHQAAHTAFSARQSAAPGAEVWLNELIWREFYHAILYHYPHVLK
                     TTLNPELRRIPWRNAPADLQAWQAGLTGYPVVDAAMRQLAALGWMHNRARMIVASFLV
                     KDLLINWQLGERWFMQSLVDGDPAANNGGWQWTAGVGADAAPYFRIFNPVLQSEKFDP
                     AGRFIRHWVPELMAVPDKYIHAPWLMPPSVQSACGVRIDHDYPAPIVDHASIRERTLA
                     AYRASAA"
     gene            complement(3802..4170)
                     /locus_tag="PKL11_03310"
     CDS             complement(3802..4170)
                     /locus_tag="PKL11_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12660.1"
                     /translation="MNLAQWGKIALMVVLTLVSGLGDSQGFIHAARVWTGSKFNWNEA
                     LLCLGGFTFGMIAYWFVIRLMNDFGITSPELQTIGWFAATIIGVALFSGDFFKWQRLD
                     QGIAIAALVAVAWLVVRKGH"
     gene            4291..5289
                     /locus_tag="PKL11_03315"
     CDS             4291..5289
                     /locus_tag="PKL11_03315"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNZ12661.1"
                     /translation="MIRNQWYAVLESDEVRSGRPVGVTRLGEKLVFWRTSAGQVVCMR
                     DLCPHIGARLSQGKVTGNALACPFHGFEYDASGQCTHLPAYGANGDIPRSLKVGVYPT
                     AEVADYIWIWWGEAETPPAPPVFFDSIGPDFSHSRFQQHWPVHYSRMAENQLDVAHLA
                     FVHHNTIGRGGRKVVDGPLVQLEGDLMNIWVYNRLDDGTPPRKADELPAPTRHPFLQF
                     RFPNIWHNWISDDVRITIAFVPVDDENCIMYGRFYQRFMRVPLLRELVNLSGRWSSEL
                     IANQDRVIVSNQWPKKTDLKMGEKIMQCDRAILTYRQHRRKLQEAAGQTVIPPSRQ"
     gene            5309..5824
                     /locus_tag="PKL11_03320"
     CDS             5309..5824
                     /locus_tag="PKL11_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12662.1"
                     /translation="MSNPLRLSRKRRSRLPRFLTLTVVPFALLLLWLFKNKPQPQKTA
                     AVDVDIPLPPDNQPEAAQPVQAAPKGAAKSRPVKTAAPTAVEPGDDLTVIEGIGPKTA
                     SVLGVMGITRYQTLAAANPADIKLVLRSAGLNFGDPTTWPEQARLAAAGDWTALKALQ
                     DTLKGGRPPVA"
     gene            6016..6738
                     /locus_tag="PKL11_03325"
     CDS             6016..6738
                     /locus_tag="PKL11_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12663.1"
                     /translation="MSDSNGTTIQGSVGTQGGDFVGRDQVNIGLNVQDLVAALRQAFP
                     AGDPRPEQVRQALLRFNAFHQALYEWKELHNALDEILNAFAPFMAQMERAQSNAQELN
                     LDDLRSLWRPVSARVDALLDFGQNIQHIGTRYQPGQTATGEKWALELADLRRATSRLL
                     GIPLAGEGDIAGRAATRTGSLQRFGLLLGSRPDWWPPLYELTQNFNSAAYLYMHLADK
                     KLRDTATELYILSKSIFGSAAP"
     gene            6735..7667
                     /locus_tag="PKL11_03330"
     CDS             6735..7667
                     /locus_tag="PKL11_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12664.1"
                     /translation="MKIATFRQLSDEHLALLQAQEQKQDVVQTATAFIEKVQAAGNVI
                     GDPRQREQLRAYLRYWASYVYDQTQTYINADLSPYTGAPVSSNSSLIWWVALPLIGLV
                     LAALIWGWKLISPIPPTPPPTPTRYIETGPTETPETFYPDLQTAAAAEVQRTAEAIDQ
                     TASAISAVQTATVGAQFLTPTPPAPGSLAVIQSPANGDNVPEVVIFEGSYVSLKPGWQ
                     IVVLAQPVNGDSLYYNMGTFVVPEGVTSGRWDIKANLSSFYNSRMAQTLLVTLAIATN
                     ESGYKMMDAAQNGILSLPEDVIWLSNTPIVLYLK"
     gene            7669..8337
                     /gene="rsmI"
                     /locus_tag="PKL11_03335"
     CDS             7669..8337
                     /gene="rsmI"
                     /locus_tag="PKL11_03335"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNZ12665.1"
                     /translation="MLYIVATPIGNLGDITQRALDTLRLVDYVISEDTRKTGILLKHF
                     EIQKPQISFREDNEQRALPRILELLESGKSLALVTDAGTPAISDPGFVLVRAVVAAGL
                     PLTSIPGPTALIAALVISGLPVHSFTFRGFPPHKRGPRLRFLQLDAESPHTLIFYESP
                     YRLKAFLEDALETYGDRRAVIANDLTKFYEVVWRGTLSELSAQVAAIELKGEFVVLIE
                     GLSR"
     gene            complement(8344..9063)
                     /locus_tag="PKL11_03340"
     CDS             complement(8344..9063)
                     /locus_tag="PKL11_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNZ12666.1"
                     /translation="MKSETRNYRFFDLVVALFVTVLIVSNIASSAKIVDWGVSLFGLP
                     LAFDAGTLLFPISYIFGDLLTEVYGFKRSRRVIWTGFACLLLSAGALWLVKVLPGEAM
                     WQEYAGQGAYEAILSGMFSGGIVLASLTAYLMGEFSNSVILARLKVATRGRWLPLRTI
                     TSTLVGEGIDSLIFVGVATLMGVFPKELFGTLVVTNYVFKVGIEVIMTPITWLAARGL
                     KRAENEDYFDTHTQFNPFGTR"
     gene            9155..9892
                     /locus_tag="PKL11_03345"
     CDS             9155..9892
                     /locus_tag="PKL11_03345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNZ12667.1"
                     /translation="MNRFFLPPASFEPDRVTFPPEVAHQMARVLRLQPGARVIALDGS
                     GSETLVELMDLTPQSAWGRALERRPAPGEPGPRLWLYLGLTQREKFEWMLQKCTETGA
                     AGFTPLISARSLVQRESEVLPKLERWQRIVQEAAEQSGRGLVPPVHPPLRLNAALTDA
                     ARHGLCLLAWEEEHTVGLNAALSAVPGQPAPHLAALIGPEGGFSAAEAAQAVAAGVQP
                     LTLGPRILRMETAAVVLTALVLHHFEE"
     gene            complement(9917..11404)
                     /locus_tag="PKL11_03350"
     CDS             complement(9917..11404)
                     /locus_tag="PKL11_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12668.1"
                     /translation="MTRKKRRETPPPPAPVVVDAALERARSLLSAEDYAALTAELERP
                     LRTAIRLNPLKAEAADLTGWASRYGWQVEPVPYCPAGWWLNSAEISPAQTPEHRLGYF
                     YIQDAASMLPVELFDFTEPPALLLDLAASPGGKTTHLISRTGDQTLTIANDASASRIQ
                     ALRIVLGGWGTTHQAVTNFPGEKFGGWFPGQFDAVLLDAPCSMESLRSTESHPMRAIS
                     TRERATLAQRQARLLTSALLAVRNGGQVVYSTCTLAPEEDEAVLDAVLRRFGRGVRVD
                     DLSERLPGPAPALDSANGSVFDPQVTRAARLWPHRFGTSGFFAARLTRLEDLPGEVEP
                     EPARPLERTGWAPLAQASAQALKQFFNDQFGFDLEGWLAANRLELWGFRERRYAIPAA
                     FLARFAGLPVQMLGLLLGEETAGEFEISHEAAARLGKLFKAGKAAMPESSIPAWLKGE
                     DLPLELSAPFSAGAVILLTGPDGQALGRGRVGPQRIKNLLPRRMG"
     gene            complement(11401..12207)
                     /locus_tag="PKL11_03355"
     CDS             complement(11401..12207)
                     /locus_tag="PKL11_03355"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12669.1"
                     /translation="MPTDLNALVAELRSLRKYRDLDLPEETVRDLLMQELPHYRSERD
                     ALKAVRQKLHNIVAPYLGDPDYTAAEAQLVTAQTAGPEAVRTACAEIMGAHASTRERV
                     PLLGEFYPRLFSLTGTPRVILDLACGLHPLGLPWMGLPEGAEYHAYDLHRPRVGLINR
                     FLELQGLPQLAEHRDILVDPPQIEADVAFFFKEAHRFEQRQRGCNRAFWQALRVRWLV
                     VSLPAADLTGHHSLVDKHRQLVYETIEGLPWEVTEIQVGNELLFCIKIIP"
     gene            complement(12209..13021)
                     /locus_tag="PKL11_03360"
     CDS             complement(12209..13021)
                     /locus_tag="PKL11_03360"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12670.1"
                     /translation="MSDPATLDTLITAVTENPRYAVIHSGLVARICAQELAKGRSTRE
                     TVKAVRSRLHQIGGAYQEAPIGYAQLAAELAVLPAERGDPALQALCRKALAQHASTRE
                     RLPLLERFFNETLAAIAPVRSVLDLACGLTPLALPWMPLAEAAPYAACDIYTDQMDFI
                     NKFFQHLRWPGEAFLCDLSSETPSRPVHLALLLKTIPCLEHLDKAIGPRLLAQINAEH
                     VLVSFPARSLGGHARGMGKTYENHFQQLIAGQPWQVRRFEFSNELAFLLSRS"
     gene            complement(13014..14144)
                     /locus_tag="PKL11_03365"
     CDS             complement(13014..14144)
                     /locus_tag="PKL11_03365"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNZ12671.1"
                     /translation="MSFTTNFRLPNGTLQFPVFLPDATFGVVRSVDSADLLNCGIQAV
                     VMNAFHLMQKPGSDVVRALGGLHRMAGWSRPIITDSGGFQAYSLIRQNPKFGKMSAQG
                     ISFQPEGANRKFQLTPEKSVQLQVGYGTDVVICLDDCTNVDAPESEQRLSVERTLAWA
                     QRAKKEYLRLMAEKKLDEDERPRLFGVIQGGGIFELRRQCAETLLEIGFDGFGFGGWP
                     LDASGGLLTEILEYTRQLVPPQFPMHALGIGHPHNVLACYRLGYGIFDCAMPTRDARH
                     NRLYTFTQPPDAHQAGLTGDWLNYIYLNDEQHVRADEPISPWCDCPVCQQYSIGYLRH
                     LFKLGDSLHLRLATLHNLRFMTLLCDRIQASLNSHAFNHSDV"
     gene            complement(14181..14798)
                     /locus_tag="PKL11_03370"
     CDS             complement(14181..14798)
                     /locus_tag="PKL11_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ12672.1"
                     /translation="MVLPPKFCSQCGKPVAAGMRFCTQCGAPIQAAPAPTPAQYTPPP
                     PPPPPAEPIVGFVLNAQRRKGFMGMGADLFNIVLTPRRVVMVMVTTKTMQDAVVLARA
                     EAKEQGKGGLGQWAAQMGWAQALARKYQTMTIEQILAEYPGSFFFENSQIHKVRVQMP
                     SDDEANHALEVIIETAGDKQKFELQMGSTKDVKTVFQQTLGGVVR"
     gene            complement(14819..17281)
                     /locus_tag="PKL11_03375"
     CDS             complement(14819..17281)
                     /locus_tag="PKL11_03375"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNZ12673.1"
                     /translation="MQFNPYTWALVLTAALSGGLALYIWQRRSSPAAAYFGLVMLTQT
                     IWAFTYGMEVASTNLSTVMFWVKLEYFGIATLSPLWLLFAVAFSGREHWLQTRWIALL
                     FIIPTLTIFIVATNELHSFYYTSVAMDYSGEIALLSIVRGPWYFVHTVYSYLCVIAGA
                     ILMLQQWWGSSAVYRRQIATILIAVLGPWVISIFYVLGWGSVGVLDLTPFGFILSSVT
                     ILYSLLREQLLDLMPLVRSSLLDELQEGVLILNERGNIVGLNDAARRFLSVDGGSLIG
                     LPVLGVLPRWTEVYPLLVSEVEAETEVHINGDDPRHLDVRVSPLRGRQGELTGRLMIM
                     HDITRHNNMEQALRESERRYRHLVEHSPEALIVHRDGFIIYANPAALRLFRAPSLESI
                     INQQVMDYVHPDYRQIVIERIRENYERGKPGEAIEEKMLRVDGEPIDVEVASVQLTFE
                     YQPATLVIVHDITESKRAMRELDETRRLAEALRDTASALNSTLNLDEVFKLILDRVRM
                     VVPADIAAVQQLDAEHKVAITTHAHDFSSQGLDTTLIAEEFEIATTANLRQMVETRQP
                     VWINDVPGDPDWKHMEGSEWIRSYLGAPILIKGQVIGFLGLTSAQPNFYCEEHAPRLM
                     AFADQAAIAIENAQLYAEVQRLAVLDDLTGIYNRRAFFDLGQREVERALRTMRPMCAL
                     FLDIDHFKQFNDAYSYQTGDQVLRLLAECLKTHLREIDLVGRYGGEEFMVLLPEADLP
                     SAMGVAERLRQQVQLIDLVTEHGKLSITISIGVATLPLRADQQPGALRSYQVRDLIDD
                     AGEALHLAKDAGRNCIASLPED"
     gene            17444..17773
                     /locus_tag="PKL11_03380"
     CDS             17444..17773
                     /locus_tag="PKL11_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNZ12674.1"
                     /translation="MNLWLSLSEADFEDRMAKAVTPVLLDFSAPWCQPCKRLEPELEK
                     LAAQLAGTAAFYTINVDEAPNLAAQLQVMGVPTVILLVNRQEAARTVGYQPLPRLLTA
                     FSPHLGS"
     gene            17950..21525
                     /gene="nifJ"
                     /locus_tag="PKL11_03385"
     CDS             17950..21525
                     /gene="nifJ"
                     /locus_tag="PKL11_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNZ12675.1"
                     /translation="MTREKVTIDGNEAVAYVAYRLSEVIAIYPITPSSNMGEHSDEWS
                     AKKIKNLWGTVPLVAEMQSEGGAAGAVHGALQTGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVSARSLAAQGLSIFGDHSDVMAARQTGWAMLASTSPQEAHDMALIAQAAT
                     LGARVPFLHFFDGFRTSHEVNKIEQLTLDDMKAMIDDDLVAAHRARALNPERPVLRGT
                     AQNPDVYFQARETVNPYYTACPATVQRAMDRFAGLTGRQYHLFDYAGAPDADRVIIIM
                     GSGAETAEETAAFLQQQGEKVGVLTVHLYRPFSIEHLLKALPATVKKIAVLDRCKEPG
                     SAGEPLYQDVLTAIAEGLDLGIAPFKTSPRVVGGRYGLSSKEFTPAMVKAIFEELKKA
                     APKNHFTIGINDDVTHSSLPYDPEFSIAAEDVVRCVFFGLGADGTVGANKNSIKIIGE
                     ETEFGAQGYFVYDSKKSGSVTISHLRFGPRPIRAPYLIGQGDANFVGCHQFSFLERLD
                     ILRYAAPGAVFLLNSIYGPEEVWDYLPREVQEAIITKKLRFYVIDAYEVAQKTGMGGR
                     INTIMQTCFFAISGVLPRDEAIAQIKKSIKKTYGKRGEAVVQSNYEAVDATLAHLSEV
                     KVPAKVTSTITRRPAVPAEAPEFVRDVLGMMISSDGDDLPVSAMPVDGTYPTGTTRWE
                     KRNIALEIPVWESDLCIQCGKCFFVCPHAAIRAKVYEPALLDNAPAAFKSTEAKFKEL
                     PGLRYTIQVAPEDCTGCGLCVEACPAKDKAQAGRKAINMAPQPPIRETEAGNWTFFLS
                     LPDLVDSPLNPGMVKNVQLLEPMFEFSGACSGCGETPYIKLVTQLFGDRMVVANATGC
                     SSIYGGNLPTTPWSTNGEGRGPAWSNSLFEDNAEFGLGMRLTLDKQTEFARELIVELA
                     AEIGETLTTELLNADQTTDAGVRAQRARVEALRSKLAGSKNPRARFLSELADALVRKS
                     VWIIGGDGWAYDIGYGGLDHVMASGRNVNILVLDTEVYSNTGGQASKSTPRAAVAKFA
                     ANGKGLPKKDLGLIAMSYGYVYVAKVAMGANDAQTLKAFLEADAYDGPSLIIAYSHCI
                     AHGIDMRKGLDQQRLAAQSGIWPMYRYNPALIDQGQNPLTIDSKEPTVSVQEYAYNET
                     RYRTLIQTDEARAEALMLQAKEDVTRRWEYYRQLAAMHYKPDEQA"
     gene            21595..22602
                     /locus_tag="PKL11_03390"
     CDS             21595..22602
                     /locus_tag="PKL11_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNZ12676.1"
                     /translation="MMVDLTTTYLGLTLKNPLVASASPLSKKVDTVRRLEDAGISAVV
                     MYSLFEEQISHESHALDHFLSHGSESFAEALTYFPDLDHYNIGPDEYLNLIRKLKQSV
                     SIPVVGSLNGVSAGGWVDHARMIEQAGADALELNIYYLPTDAALTAAELEQAYVDLVA
                     AVRKQIRIPLAVKLSPYFTALPHFASALVGAGANALVLFNRFYQPDLDIETLEVLPTL
                     DLSTSAELRLPLRWTALLYGRIKADLALTTGVHTATDVVKATMAGANVTMLASELIAH
                     GPARAAEIAADLEQWLVKYEYTSIKQMRGSMSQQNVAEPAAFERANYMKALQSFDNRL
                     R"
     gene            complement(22683..22934)
                     /gene="infA"
                     /locus_tag="PKL11_03395"
     CDS             complement(22683..22934)
                     /gene="infA"
                     /locus_tag="PKL11_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNZ12677.1"
                     /translation="MPKEEDKIQVEGTIVEALPSTQFRVRLDNGHVVLAYLSGRMRKY
                     YIRILLGDRVRVEMSPYDLTRGRIVYRSKKPNENLPLEA"
     gene            complement(22972..23160)
                     /gene="rpsU"
                     /locus_tag="PKL11_03400"
     CDS             complement(22972..23160)
                     /gene="rpsU"
                     /locus_tag="PKL11_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNZ12678.1"
                     /translation="MLRSNESQDQLLKRFRKKVVKSGVLSTVRRKRWFVSKSELRRVE
                     KKKAIRRIKRRQVGRTMD"
     gene            complement(23419..>24009)
                     /locus_tag="PKL11_03405"
     CDS             complement(23419..>24009)
                     /locus_tag="PKL11_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNZ12679.1"
                     /translation="KTSLTERLGERMGWRTAYESVADNPYLPDFYADMQAWSFHLQVF
                     FLGHRAEQHMELAADPRPAIIDRSIYEDAFIFARALQIMGNLSERDYATYRQLFDLVV
                     KKLPPPSLLIHLKAPVEVLMKRIHRRGRAIESSISADYLTLLESLYDEWMQAFDLCPV
                     LTIRTDDLDFVHHSKALDEVIERIQDKLAGKEELFL"
BASE COUNT         4952 a         7676 c         6371 g         5010 t
ORIGIN      
        1 atcgagtagt ttcatttgcg caagggatta atctggagta cactttattc tatgaacgta
       61 gctttacctg cctttctaaa ggtatttttc tgggattgcg atttttccgc attgacattc
      121 agtgagcagc ccgattttat catccgtcgt ttgctccagg cgggaaactg ggaagcaatc
      181 agttggctga ggactgaact gggtgatgcc gcgttacgtc agtggattga gcagcacaat
      241 ggcgccggcc ttcagccgcg ccagttgcgc ctgtgggaag tcgttctgga tctgcctcat
      301 gccagggtat ctcgctgggt acgcgcggca gcatctcaac cctgggaagg aaggctggcg
      361 cgatgaactt tacaaacgcc ggtataaacc gagagcagga atccgttctg gcccagatag
      421 ggggctggct cacagagcag ggatactatc tagcaggtgg cacagccctt gcaatttact
      481 atggtcatcg ccaatctgtg gatttagatt ggtttacaac agcacacatc cctgatgttt
      541 tggctttggc acagcgttta cgagaaactg gtatgccttt cgaaacaggt cactttgcgc
      601 ccggaaccct ttatggtcag attggtaatg tgcgcatcag cctcattgaa ttttgctatc
      661 ccttgctaaa cccgctggaa gtttggcctg ctaaccagac ggcccttgcc agcctggatg
      721 atctggtatg tatgaaatta tctgccattg cgcagcgtgg cacccgcaaa gatttttatg
      781 acacccacct gttgctgggt cgccattgct cgttggccga tgctttgtct ctttttcaga
      841 aaaaattcaa tgtcagtgat atcagcccgg ttctatatgg actggcttat tttgatgatg
      901 ccgagaaaga accagatcca atattgcttg agaagatcac ctggaaacag gtcaagaaag
      961 ctattcttca gtggctgaaa gaatactctg gcgcagttga ttgatgagct tatcaaggtt
     1021 aaaattggaa cgcttgcagt tctgatataa ttacctgcgt gtccacatcc accaagcccg
     1081 gcgcgctcta ccgcgccatg cacaacctgt tcctgctcac cgcagtcttt ggcctgatca
     1141 tggggttgat taacctgttg ggcctggtca gcggcttcaa ccccacctat ctcgccgatg
     1201 gcctgttcaa cctggccttt gcggctgcca atttcatcgg ctaccgtctg atggccagcc
     1261 gcagccgcac cgtcatctgg ctgcaggtgg gcatcatcat tgccggactg ttgtatggcc
     1321 cgctggtggg gcgcgggctg gcgctccaat atcccatcat tggcctgatt tacctgttca
     1381 tcctcatcac cttgcgtcgg cgcggcgaaa tccaataacc ccgtcttagc ctgtccaatc
     1441 ttcctcactt gattcgctcc cccggaacgg ctataatcca acccatgaca cctgaaccta
     1501 cccgtgcccg cgtaaacccg gaaacagtgg atacaacccc gctgcgccgg ccctcctgga
     1561 ttaaagtgcg cgcccccagc ggcgaaactt atgagcagct ccaggcgctc atgcgtcgca
     1621 aagcgctgca taccgtttgc gaagaagctg cctgccccaa tatgggcgaa tgctggggct
     1681 ccggcaccgc caccttcctg atgcttggca atgtctgcac ccgcacctgc ggcttttgtg
     1741 acatccaaca cggacgcccg cccgcgcttg attggctcga agcggagcgt gtggctcagg
     1801 cggtggcttc tatggaactt cagcatgctg tcattaccag cgtcaaccgt gatgaccgcc
     1861 gggatggcgg cgcccccatc tttgccatgg tcattcgccg catccgccaa ctgcgcccag
     1921 gctgctcggt cgaagtcctc atccccgact tcaaaggctc ggctgaagct ctgcgcatgg
     1981 ttgtggaagc tcgccccgag attttgaatc acaatgtcga gacggtgccg cggctcttca
     2041 aaactgtcca gccgcaagat cattacgaat gggcccgcgc caccctcacc aacgccaaac
     2101 gcatggaccc cgatatcctc actaaatccg gcatcatggt cggcctgggc gagacgctgg
     2161 acgaagtcaa ggctgtgatg gacgacctgc gtacctgggg cgtgcaaatt ctgaccattg
     2221 ggcagtacct gcagcccagc cgcaagcatt tgcccatggc gcgttactac acccttgaag
     2281 aatttgccga actgaaagaa tatggcctgg gcattggctt tagctgggtt gaaagcgcac
     2341 ccctcgtgcg ttcttcttac catgccgccg aacaggtgcg cgccctcagc accattcaca
     2401 agcgcctcta cggagttggg cagggcggct gatggcggct gtcctttggt ggctgcgccg
     2461 cgacctgcgc ctggcagata accccgcctt gcagcgcgct ctgtcgttcg gcgccggggt
     2521 tgtcccggtc ttcattctcg atccccattt gctcaatcag cctgccccct tgcggcaggc
     2581 ttttcttttt gatggtctgc gctccctgga tgccgacctg cgtgcccgcg gcagccgtct
     2641 gattctgcgt caaggccagg cggaggttga actggcccgc ctgctggctg agaccggcgc
     2701 cacccgcatc atcgccgaag aagattacag cccctatgcc cgcgcccgtg atgccgctgt
     2761 ggcagcccaa ctgccgctgg agcaggtgct gggtctgacg gtttttcacc ctgcccatgt
     2821 gcttaaagca gatgggcaac cttatcgcgt gttcaccccc ttcagccggg cctggaaggc
     2881 tctgcccttc cctggcgccc cctccccggc cccggctgcg cttccgcccg tcccccccga
     2941 tctcacctcg ctgcccattc cctcggtcag cccctcaacc gccttccccg caggtgaagc
     3001 tgaagcccac cgccgcctga cagattttgc cgccggaccg ctgtctgatt atgccgacgc
     3061 ccgtaaccgg ctcgacctgg acggcacctc ggcgctttca ccatacctgc ggctgggcat
     3121 gctctcggcg caccaggcgg ctcacaccgc cttttctgcc cggcagagcg ccgctccggg
     3181 tgccgaagtc tggctcaatg aactgatctg gcgtgagttt taccatgcca ttctttatca
     3241 ttatccccat gtcctcaaga cgaccctcaa tccggaactg cgccgcatcc cctggcgcaa
     3301 tgctcctgcc gacttacagg cctggcaggc gggccttacc ggttacccgg tggtggacgc
     3361 agccatgcgc cagcttgccg cgctgggctg gatgcacaac cgcgcccgca tgatcgtggc
     3421 ctctttcctg gtcaaagatc tgctcatcaa ctggcagctc ggcgagcgct ggttcatgca
     3481 atccctggta gatggcgacc ccgcggccaa taatggcggc tggcagtgga cggctggcgt
     3541 tggcgccgat gccgccccct atttccgcat cttcaacccg gtgctgcaaa gcgaaaaatt
     3601 tgaccctgcc gggcgattca tccgccactg ggtgcctgaa ctgatggctg tcccagacaa
     3661 gtatatccac gccccctggc tgatgccccc gtccgttcaa tcagcctgtg gcgtgcgcat
     3721 tgaccacgac taccctgcgc caattgtgga ccacgcatca attcgtgaac gtacactggc
     3781 ggcgtatcgc gcttcagcgg cctaatgccc ttttcgtacc accagccacg ccaccgcaac
     3841 cagcgcggca atggcaatgc cctggtccaa tcgctgccat ttgaagaaat caccgctgaa
     3901 cagcgccacg ccaatgatgg tggcagcaaa ccagccgatg gtttgcagtt ccggtgaagt
     3961 gatgccaaaa tcattcatca ggcggatgac aaaccagtaa gcaatcatcc caaatgtgaa
     4021 tccacccagg cacagcagcg cctcattcca attaaacttg ctgccggtcc acacccgcgc
     4081 tgcatggata aaaccctgcg aatcacccag tccagagacc agcgttaaaa caaccattaa
     4141 tgcaattttg ccccattgag caaggttcat aagattgact ccattggaat attgagacaa
     4201 acccggttca aaactcctcc ggtctggccg cgccagcgca cggcattggg attataatcg
     4261 aagtgtattg tttatgtctc cggagaacac atgatccgca atcaatggta tgccgtgctg
     4321 gaatctgatg aggttcgctc aggccgcccg gttggcgtta cccgcctggg cgaaaaactg
     4381 gttttctggc gcacctcggc tggtcaggtt gtctgtatgc gcgatctgtg ccctcatatt
     4441 ggcgccaggc tcagtcaggg caaagtaact ggaaacgccc ttgcttgccc cttccacggc
     4501 tttgaatatg acgcctccgg tcagtgcacc catctaccgg cttatggcgc caacggcgac
     4561 atccctcgct cgctcaaagt tggggtctat cccaccgccg aagttgccga ttacatttgg
     4621 atctggtggg gcgaagctga aaccccgccc gctccgcccg tctttttcga cagcatcggt
     4681 ccagatttca gccacagccg ttttcagcag cattggccgg tgcactactc gcgcatggcc
     4741 gaaaatcaac tggacgtggc ccacctggca tttgtccatc acaacaccat cggccgcggt
     4801 gggcgcaaag tggtggatgg ccccctggtg cagcttgaag gcgacctgat gaatatctgg
     4861 gtctataacc gcctcgacga cggcaccccg ccccgcaaag ccgacgaact ccccgcgcca
     4921 acccgccacc ccttcctgca gttccgcttc cccaatatct ggcataactg gattagcgac
     4981 gatgtgcgta tcaccatcgc ctttgtgccg gtggatgacg aaaactgtat catgtatggc
     5041 cgcttttatc agcgttttat gcgtgtgccg ctgctgcgcg aactggtcaa cctatccggt
     5101 cgttggagca gtgaactcat tgccaatcaa gaccgcgtga ttgtctcaaa tcagtggcct
     5161 aaaaagaccg acctgaaaat gggggaaaag atcatgcagt gcgaccgggc gattttgacc
     5221 tatcgccagc accgccgcaa actgcaagaa gccgccgggc agacggttat tcccccgtcc
     5281 cggcaatgaa tttcccctgg aggctatgat gtctaatccc ttgcgcttat cccgaaaacg
     5341 ccgctcacgc ctgccccgtt ttttaaccct gactgtggtg ccctttgccc tgctcctgct
     5401 ctggcttttc aagaacaagc cccagccgca aaaaactgcc gctgtcgatg tggatatccc
     5461 cctgccgccc gacaaccagc cagaagcagc tcagcccgtt caggccgcac ccaaaggcgc
     5521 cgcgaaatcg cgcccggtaa aaactgccgc gcccacagcg gtcgaacctg gtgatgatct
     5581 gaccgtgatt gaagggattg gccctaaaac cgcctctgtg ctcggggtca tgggcatcac
     5641 gcgctatcaa accctggctg ctgccaaccc ggcggatatt aagctggtgt tgcgttcggc
     5701 tggtctgaac tttggcgacc ccaccacctg gcccgaacag gctcgcctgg cagcggctgg
     5761 cgattggacc gcactgaaag ctttgcagga tacgctcaaa ggtggccgcc ctccggtcgc
     5821 ctgagatggt tgggcttctt agaaactgag tacgctttaa atttggagtg cgggcttcag
     5881 cccgctttcc atttagtgta tgagtttcgc cctgttactc atccggaatg cagtctgcct
     5941 cccaccattt agcgcgataa taaatctatt ggaactcttg ccctctctgg gccgtcctga
     6001 ataaaggata ttctgatgag cgacagcaac ggcacaacca ttcaaggcag tgtaggcacc
     6061 cagggcggcg actttgttgg ccgcgaccag gtgaacattg gcttgaatgt tcaagacctg
     6121 gttgcggctt tgcgtcaggc gtttcccgcc ggcgatcccc gccccgagca ggttcgccag
     6181 gctttgctgc gcttcaatgc ttttcatcag gcactgtatg agtggaaaga actgcacaac
     6241 gccctggatg aaatcctcaa tgcttttgcc cccttcatgg cgcaaatgga gcgtgcccag
     6301 tccaacgccc aggaattaaa cctggatgac ttgcgcagcc tgtggcgccc ggtctctgcc
     6361 cgggtggatg ccctgctgga ttttgggcag aatattcaac acattggcac ccgctatcag
     6421 cccggccaga cggctactgg cgaaaaatgg gccctggaac tggccgacct gcgccgcgcc
     6481 acctcccggc tgctgggcat ccccctggca ggtgagggtg atatcgctgg acgtgccgca
     6541 acccggactg gttcgctgca gcggtttggc ctgctgctgg gctctcgccc ggattggtgg
     6601 ccgccgctct acgaactgac ccaaaacttc aacagcgccg cctaccttta catgcacctg
     6661 gccgacaaaa aactgcgaga tacagccacc gagctgtata tcctctccaa atccatcttt
     6721 gggagtgccg caccatgaag attgcgacct ttcggcagtt atcggatgag catctggctt
     6781 tgctgcaggc gcaagagcaa aagcaggatg ttgtccaaac agccactgct tttatcgaga
     6841 aagttcaggc agccggcaat gtgattggcg atccccgcca gcgtgagcag ctccgggctt
     6901 atttgcgcta ctgggccagt tatgtctatg atcagacaca gacgtatatc aacgcagatc
     6961 tctcgccgta cacaggcgca ccggtgtcca gcaattccag cctgatctgg tgggtggctc
     7021 tgcctttgat tggattggtg ttggctgcgc tgatttgggg ttggaagttg atcagcccga
     7081 ttcccccaac gccccccccc acaccaacca ggtacattga aaccggtccc acggaaaccc
     7141 ccgaaacctt ttacccggac cttcagacgg ccgccgccgc tgaggtgcag cgcaccgccg
     7201 aggctatcga tcagacagcc tccgccattt cagcggttca aactgctacg gtcggtgctc
     7261 agtttctgac ccccacgccc ccggctcccg gctcgctggc ggttattcaa tccccggcaa
     7321 atggcgataa cgtgcccgag gtggtgatct ttgagggcag ttatgtcagc ctcaaaccgg
     7381 gctggcagat tgtggtgctg gcccagccag tgaacggcga ctcgctctac tacaacatgg
     7441 gcacgtttgt ggtgcccgaa ggcgtgactt cgggccgttg ggatatcaaa gccaacctga
     7501 gcagcttcta taacagccgc atggcccaaa ccctgctggt tacccttgcc atcgctacca
     7561 acgaatctgg ctataaaatg atggacgcgg cccagaacgg catcctctca ctgcctgaag
     7621 atgtgatctg gctgtccaat acaccgattg tcctttacct gaaataacat gctgtacatt
     7681 gtcgcaaccc ccattggcaa cctgggtgat attacccagc gcgccctcga taccctccgg
     7741 ctggtggatt atgtcatcag cgaagatacc cgcaagacgg gcatcctgct caaacatttt
     7801 gagattcaaa aaccacagat ctcgtttcgt gaagataatg agcagcgcgc cctgccgcgc
     7861 attttagaac tgctcgaaag cggcaagtcg cttgccctgg tcaccgatgc cggcactccg
     7921 gccatttcag accctggttt tgtgctggtg cgcgccgtgg ttgctgctgg cctgcccctg
     7981 acctctatcc ccggccccac tgctctgatt gcagcgctgg tcatctctgg cttgccggtg
     8041 cacagcttca cgtttcgcgg cttcccccca cacaaacgcg gcccgcgcct gcgctttctg
     8101 cagttggatg ccgaatcgcc tcacacactg attttttacg aaagccccta ccgcctcaaa
     8161 gccttcctcg aagatgcact ggaaacctac ggtgaccgcc gggcagtcat cgccaacgac
     8221 ctgaccaaat tctacgaggt cgtctggcgc ggcaccctgt ccgaactgtc ggcgcaagtt
     8281 gctgccatcg aacttaaagg cgagtttgtg gtgctgattg agggcctgtc gcgctgagct
     8341 tggctaccgg gtcccaaatg gattgaactg cgtgtgcgtg tcgaagtaat cttcgttctc
     8401 agcgcgcttc agcccgcgtg cggccagcca ggtgattggt gtcataatga cttcgatgcc
     8461 caccttgaac acataattgg tcacgaccag tgtgccaaat aattctttgg ggaacacgcc
     8521 catcagcgta gccacaccca caaaaatcag cgagtcgatc ccttcaccta cgagtgtgct
     8581 ggtgatggtg cgcagcggca gccagcgccc gcgggtcgcc accttcaacc gtgccagaat
     8641 caccgagttg ctgaactcgc ccatcaggta ggccgtcagg ctggctaaca caatcccgcc
     8701 gctgaacatg ccgcttaaaa tcgcctcgta agcaccctgt ccggcgtatt cctgccacat
     8761 ggcctcgccc ggcagcactt tgaccagcca cagcgccccg gcgctcagca gcaggcaggc
     8821 gaacccggtc caaatcaccc gccgcgagcg cttgaaccca tagacctcgg tcagcaggtc
     8881 gccaaagata tagctgatcg ggaagagcag cgtcccggcg tcaaacgcca gcggcagccc
     8941 aaacaacgaa acaccccagt ccacaatttt ggccgatgag gcaatgttgc tgacaatcaa
     9001 aacagtgacg aacaacgcca ccaccagatc aaaaaagcga taattgcgag tttctgactt
     9061 catacgttca ctttcctgta ttttttacac cggttgcccg gcggggtaca gtataatcga
     9121 aagtgttggt ttgtgcctta aggatttttc accgatgaac cgtttttttc ttccccccgc
     9181 cagttttgag cctgaccggg tcaccttccc gccagaggtt gcccatcaga tggcccgcgt
     9241 tttgcgcctc caacccggcg ctcgggtcat tgcgctggat ggctctggca gcgaaaccct
     9301 ggtcgaactt atggatctta ctccacagtc agcctggggc cgcgccctcg agcgccgccc
     9361 ggcgcccggc gagccggggc cccgcctgtg gctctacctg ggcctgaccc agcgtgaaaa
     9421 atttgagtgg atgctgcaga aatgtaccga gaccggtgcg gccggcttta ccccgttgat
     9481 ttcggcgcgc agcctggtgc agcgtgaaag cgaagtactt cccaagctgg agcgctggca
     9541 gcgcattgtg caagaggcgg ccgagcagtc tggccgcggt ctggtcccgc cggtgcaccc
     9601 gccgctgcgc ctcaatgccg cgctgactga tgctgcccgg catggcctgt gcctgttggc
     9661 ctgggaggaa gagcacaccg ttggcctgaa tgccgccctg tctgctgtgc cggggcagcc
     9721 agccccgcat ctggccgcgc tgattggccc cgaaggcggc ttctcggctg ctgaagcagc
     9781 ccaggcagtt gctgccgggg tgcagcccct cacgcttggg ccgcgcatcc tccgcatgga
     9841 aaccgccgcc gttgtcctga cggcgctggt gctgcatcat tttgaggagt agcctctacc
     9901 agaaaccaga cccggcttag cccatccgcc gcggcagcag atttttgatc cgctgcggtc
     9961 ccacgcgccc gcgccccagc gcctgcccat ccgggccggt cagcaaaatt accgcgcccg
    10021 ctgagaacgg cgcactcaat tccaacggca ggtcttcccc cttcagccac gccgggatgg
    10081 agctctctgg catggcagct ttcccggcct taaataattt gcccagccgg gcagccgcct
    10141 cgtgcgaaat ttcaaactcg cccgcggtct cttcgcccag cagcaagccc aacatttgca
    10201 ccggcagccc ggcaaaccgc gccagaaacg ccgccggaat cgcataccgc cgctcccgga
    10261 atccccacag ttccagccgg ttggctgcca gccagccttc caggtcaaac ccgaactggt
    10321 cgttaaaaaa ttgtttcagc gcctgggcgc tggcctgtgc cagtggcgcc cagcccgtgc
    10381 gttccagtgg gcgggcgggt tctggctcca cttcgccggg taaatcttca agtcgcgtca
    10441 gccgggccgc aaaaaagccc gatgtgccaa agcggtgcgg ccagagccgc gccgcccgtg
    10501 tcacctgtgg gtcaaaaaca gatccgtttg cgctgtccag ggctggcgcc gggcccggca
    10561 gccgctctga gagatcatct acgcgcaccc cgcggccaaa ccgccgcagc acagcatcca
    10621 gcacggcctc gtcttcctcc ggcgccagtg tacaggtgga ataaaccacc tgcccgccgt
    10681 tgcgcaccgc cagcagggcg ctggtcaaca gccgggcctg ccgctgggcc agggtggccc
    10741 gctcccgcgt agaaatggct cgcatggggt gtgactcggt ggagcgcaag ctctccatgc
    10801 tgcacggcgc gtccaaaagc accgcgtcaa actgccccgg aaaccagccg ccaaactttt
    10861 cgcctggaaa attcgtcacc gcctgatgcg ttgtgcccca accgcccagt acaatccgca
    10921 gcgcctgaat tcggctggcg ctggcgtcgt tggcaatcgt caatgtctgg tcgccggtgc
    10981 ggctgatcag gtgagtggtc ttgccgcccg gcgaagccgc caggtcgagc agcagggccg
    11041 gcggctcggt aaagtcaaac aactccaccg gcagcatcga agccgcatct tgaatgtaga
    11101 aatatcccaa ccggtgctcc ggcgtttgcg ctggagaaat ttccgccgag ttcaaccacc
    11161 agccagccgg gcagtagggc accggctcta cctgccagcc atagcgactg gcccagccgg
    11221 ttaaatcagc cgcctctgct ttcagcggat ttaaccggat tgccgtgcgc agtgggcgct
    11281 ccagttcagc cgtcagcgca gcataatcct ccgcactgag caggctgcgg gcgcgctcca
    11341 gcgcggcatc aaccacaacc ggcgctgggg gtgggggcgt ctcgcggcgt tttttgcggg
    11401 tcatgggatt attttaatac aaaacaacag ttcgttcccc acctggatct ctgtcacttc
    11461 ccatggcagc ccttcgatgg tctcatagac cagttggcgg tgcttgtcta ccagcgagtg
    11521 atgccccgtc aggtcggcgg ccggcagcga gaccaccagc cagcgcaccc gcagcgcctg
    11581 ccagaaagcc cggttgcacc cccgctgccg ctgctcaaac cgatgcgctt ctttgaagaa
    11641 aaacgccaca tcagcctcaa tctgcggcgg atcgacgagg atgtcccggt gttcagccag
    11701 ttgcggcagc ccctgtaatt ccagaaagcg gttaatcaac cccacccgtg ggcggtgcaa
    11761 atcataggcg tgatattctg ccccctccgg cagccccatc cacggcagcc ccagcggatg
    11821 cagcccgcac gccaggtcga ggatcacccg cggcgtaccc gtcaagctaa acaggcgcgg
    11881 gtaaaattca cccagcagcg gcacccgttc ccgggtggag gcatgcgcac ccatgatttc
    11941 agcacaggcg gtgcgcactg cctctggtcc ggcagtctgc gccgtaacaa gctgcgcctc
    12001 cgccgcggtg tagtcgggat cacccaggta gggcgccaca atattgtgca gcttctgccg
    12061 caccgctttc agggcatccc gctcgctgcg ataatgtggc agttcctgca tcagtaggtc
    12121 gcgtacggtc tcttccggca ggtccaggtc acggtatttg cgcaggctgc gcagttcagc
    12181 taccagagca ttcaggtcgg tgggcatctt agctgcggct caataaaaag gccagctcgt
    12241 tggaaaactc aaaccggcgc acctgccagg gctgcccggc aatgagctgt tggaagtggt
    12301 tttcataggt cttgcccatc ccgcgggcat gcccacccag tgaccgcgcc gggaacgaga
    12361 ccagtacatg ttccgcgttg atctgcgcca gcagccgcgg tccaatcgct ttatccagat
    12421 gctccaggca ggggatggtc ttcagcagca gcgccagatg aaccgggcgg gagggcgttt
    12481 cgctgctcaa atcgcagagg aaagcctcgc ctggccagcg caggtgttga aaaaacttat
    12541 taatgaagtc catctggtcg gtgtaaatgt cgcaggccgc atagggcgcc gcttctgcca
    12601 gcggcatcca cggcagggcc aggggcgtca atccgcacgc caggtccagt actgaacgca
    12661 caggtgcaat tgccgccagc gtctcgttaa aaaaacgctc cagcagcggc agccgttccc
    12721 gggtggaggc atgttgggct agcgccttgc ggcacagggc ttgcagagcc gggtctcccc
    12781 gttcagcagg cagaaccgcc agttcagcag caagctgcgc atagccgatt ggcgcttctt
    12841 gataagcgcc gccaatttgg tgcagtcggc tccgcaccgc cttcaccgtc tcacgggtac
    12901 tgcgcccctt ggccagctcc tgtgcgcaaa tccgcgccac caatccggag tgaatcacgg
    12961 catagcgcgg gttttcggtc accgctgtaa tcagggtatc caacgtcgca ggatcagaca
    13021 tcggagtgat tgaaggcatg cgaattgagg ctggcctgaa tcctgtcaca cagcagcgtc
    13081 ataaaacgca ggttgtgcag cgttgccagc cgcaggtgca ggctgtcgcc cagtttgaac
    13141 aggtgccgca ggtagccaat tgaatactgc tggcaaaccg ggcagtcgca ccagggcgaa
    13201 atcggctcgt cggcgcggac gtgctgctca tcgttcaggt aaatgtaatt caaccagtcg
    13261 ccggtcaggc cggcctggtg cgcatctggc ggctgggtaa aggtgtacag ccggttgtgc
    13321 cgggcatcac gcgtgggcat ggcgcagtca aatattccat agcccagccg gtaacaggcc
    13381 agcacattgt gcgggtgccc gatgcccagc gcgtgcatgg gaaactgcgg cggcaccagt
    13441 tggcgggtat attccagaat ctcggtcagc agcccgccgg aggcatccag cggccagccg
    13501 ccaaacccaa acccatcaaa gccaatctcc agcagcgttt cagcacactg cctgcgcagc
    13561 tcaaaaatcc cgcccccctg aatgacaccg aacaatcgcg ggcgttcatc ctcgtccagc
    13621 tttttttcag ccatcaaacg caggtattct ttttttgcgc gctgcgccca tgccagggtt
    13681 cgttccaccg agagccgctg ctcgctttcg ggcgcgtcca cgttggtgca gtcatccagg
    13741 cagatgacca catccgtgcc atacccaacc tgaagctgca cactcttttc cggtgtcagt
    13801 tgaaatttcc gatttgcccc ctccggctga aaactgattc cctgggcgct catcttgcca
    13861 aacttgggat tttgccggat gagcgaatag gcctgaaacc cgcccgaatc ggtaatgatg
    13921 gggcgcgacc agcccgccat gcggtgcagc ccacccagcg cccgcaccac atcggatccc
    13981 ggcttttgca tcaggtgaaa tgcattcatc accacagcct gaatcccgca gtttagcagg
    14041 tcagccgaat caacactgcg cactacccca aaggtggcgt ctggcagaaa aaccgggaat
    14101 tgcagcgtac cgttgggcag cctgaagttc gtggtaaagc tcatgcttgc attatacccg
    14161 aaggacgctg cccgctgcgc ttaacgcacc actccgccca gcgtttgctg gaagaccgtc
    14221 ttaacgtctt tggtgctgcc catttgaagc tcaaattttt gcttatcgcc ggccgtttca
    14281 atgatcactt ccagcgcatg gttggcttcg tcatccgatg gcatttgcac ccgcaccttg
    14341 tggatttgag aattttcaaa aaagaagctg ccggggtatt cagccagaat ttgctcaatt
    14401 gtcatggtct gatatttccg cgccagggcc tgggcccagc ccatctgtgc cgcccattga
    14461 cccaatccgc ctttgccctg ctccttggct tctgctcgcg ccagcaccac cgcatcttgc
    14521 atcgtcttgg tggtgaccat caccatcacc acccgccggg gcgtgagcac aatattaaac
    14581 aggtcagctc ccatgcccat aaaacccttg cgccgctggg cgttcagcac aaatcccaca
    14641 atcggctctg ccggtggggg tggggggggc ggcggggtat attgcgccgg ggtgggtgct
    14701 ggtgcagcct ggatgggcgc accgcactgg gtgcagaagc gcatccccgc ggcaacgggc
    14761 ttaccacatt gactgcagaa tttcggtggc agaaccatga tactacctcc ttggcattct
    14821 aatcttcggg caggctggca atgcagttgc gcccggcgtc tttggccagg tgcagcgctt
    14881 cgccggcatc gtcaattaga tcgcgcacct gataactgcg cagtgctccg ggctgctggt
    14941 cagcccgcag cggcagggtc gctaccccaa tgctgatggt gatcgaaagc ttgccatgct
    15001 ccgtaaccag gtcaattaac tgcacctgct gacgcagccg ttcagccacg cccatggcgc
    15061 tgggcaggtc ggcttcgggc agcagcacca tgaactcttc gccgccgtac cgccccacca
    15121 gatcaatttc gcgcagatgg gtcttaaggc actctgccag cagccgcaga acctggtcgc
    15181 cggtctggta actataggca tcattgaatt gtttgaagtg gtcaatatcc agaaacagcg
    15241 cgcacattgg gcgcatggtg cgcagggcgc gctcaacctc acgctgaccc agatcaaaga
    15301 atgcccggcg gttataaata cccgtcaaat catccagcac agccagccgc tgcacttcag
    15361 cgtacaactg cgcattttca atggctatgg cagcctggtc ggcaaaagcc atcagccggg
    15421 gcgcgtgttc ttcacagtag aaattaggct gcgcactggt caatcccaaa aagccaatca
    15481 cctgcccctt gatgaggatc ggcgccccca ggtaggatcg aatccactcg ctgccctcca
    15541 tatgtttcca atcagggtcg ccgggcacgt cgttaatcca caccggctga cgggtttcta
    15601 ccatctggcg caaattggcg gtagtggcaa tttcaaattc ctcggcaatc agtgtggtgt
    15661 ccaacccctg actcgaaaaa tcatgcgcat gggtggtgat cgccaccttg tgttcagcat
    15721 ccaactgctg aacggcagca atatcggcgg gcaccaccat ccgcacccga tccaaaatga
    15781 gcttaaaaac ctcatccagg ttcagggtgc tgtttaatgc cgaagccgta tcgcgcagcg
    15841 cttcagcaag ccgccgggtt tcgtccagtt cccgcatggc gcgcttgctt tcagtaatgt
    15901 cgtgcacaat caccagtgtg gcaggctgat attcaaatgt tagctgcaca ctggcaactt
    15961 ccacatcaat cggttcgcca tctacccgca gcattttctc ttcaatcgct tcgccgggtt
    16021 tgccccgctc atagttttct cgaatccgct caatcacaat ttggcggtaa tctggatgga
    16081 cataatccat cacctgctgg ttgataatac tctccagcga tggcgcccgg aagagccgca
    16141 gcgctgccgg gttggcataa atgatgaagc catcccggtg aacaatcaat gcttcaggcg
    16201 aatgttccac caggtggcgg tagcggcgct cgctttcccg cagagcctgc tccatattgt
    16261 tgtggcgggt gatgtcgtgc ataatcatca agcgcccggt caattcgccc tgcctgcctc
    16321 gcagcggcga aacccgcaca tccaggtggc ggggatcgtc tccgttgatg tgcacctcgg
    16381 tctcagcttc aacctctgaa accagcaggg gatacacttc agtccagcgc ggcagcacgc
    16441 ccagcaccgg caggccgatc aacgatcctc catcaacact taaaaagcgc cgtgccgcgt
    16501 cattcagccc cacaatattg ccgcgctcat taaggatcaa aaccccctcc tgcagttcat
    16561 ccaatagcga gctgcgcacc agcggcatca aatccaacaa ctgctcccgc agcaggctgt
    16621 acaggatggt aacactcgac aggataaatc caaaaggcgt caggtccagc acccccacgg
    16681 aaccccatcc caacacataa aaaatgctga tgacccaggg tcccagcacg gcaatcaaaa
    16741 ttgtcgcaat ctgacgccgg tacacagccg atgaccccca ccactgctgt aacattaaaa
    16801 tagccccggc aattacacac aggtaagagt aaacggtatg cacaaagtac cagggcccgc
    16861 gcacaatgct cagcagggca atctcgccgc tgtaatccat ggctaccgat gtgtagtaga
    16921 acgagtgcag ttcattggtc gcgacgatga agatcgtcaa agtcgggata ataaataaaa
    16981 gcgcaatcca gcgggtttgc agccagtgtt cccgcccgct gaaggcaacc gcaaacagca
    17041 gccacagcgg cgaaagagtg gcaatgccga agtattccag tttgacccag aacatcacgg
    17101 tggataaatt ggtgctggcg acctccatgc cataggtgaa cgcccagatg gtctgcgtca
    17161 gcatcaccaa cccaaaatat gcagccgccg gtgaagaccg ccgctgccag atgtataagg
    17221 ccagtccgcc tgaaagtgcc gcagttaaca ctaaagccca ggtatagggg ttaaactgca
    17281 tccaaacctc aatggataaa taataccgct ctggagatgt cgggcggata tccgccctta
    17341 tacagatatt gtagcacggt gcgcacttaa agcaattcaa attatcctca cattttttgt
    17401 caactcgcgt tatacttcct ctgtttggtt catggaggcg ttgatgaatt tgtggttatc
    17461 cctgagtgag gctgattttg aagaccggat ggcaaaagct gttacgccag tcctgctcga
    17521 tttcagcgca ccctggtgcc agccctgcaa gcgtctggag cccgaactcg agaagctggc
    17581 cgcccaatta gctggcacag ctgccttcta caccatcaat gtggatgagg cccccaacct
    17641 ggctgcccaa ctccaggtca tgggtgtccc aactgtcatc ctattggtca acagacagga
    17701 agccgcgcgc acggtggggt atcaaccctt gccgcgcttg ctcaccgctt tttctcccca
    17761 cctgggatcc tgatgcgcgg caattcttcc tgtatcttaa gtgaccaata taacttactc
    17821 caatcggagt taaaggataa acttttaagg atgagctttt ctcaactgtc ttgacgcgtc
    17881 tgaattcccc ggcagtgccc ctgtccgggc agaagcgtct ttttacaata taaccaaatg
    17941 gagtgtgcta tgacgcgcga aaaggttacg attgacggca acgaagccgt cgcatatgtg
    18001 gcgtatcggc tgagcgaagt aatcgctatc tacccgatca ccccttcatc caacatgggc
    18061 gagcattctg acgaatggtc ggccaagaag attaagaacc tgtggggcac ggtgccgctg
    18121 gttgccgaaa tgcaatccga aggcggcgcg gctggcgcgg tgcatggcgc actgcaaacc
    18181 ggtgccctca ccaccacctt cacggcctcg cagggcctgc tgttgatgat ccccaacatg
    18241 tacaagattg ctggcgagct gacctccacg gtcttccacg tctccgcccg ctctctggcg
    18301 gctcaggggc tctcaatctt cggcgatcat tccgatgtca tggctgcccg ccagaccggc
    18361 tgggccatgc tggcttccac ctccccgcag gaagcccacg acatggcact gattgcccag
    18421 gctgccaccc ttggcgcccg cgtgcccttc ctgcacttct ttgatggctt ccgcacctcg
    18481 cacgaagtca acaaaattga gcagcttacc ctggacgaca tgaaggccat gattgatgat
    18541 gacctcgttg cagcgcaccg cgcccgggcc ctcaaccccg aacgccccgt gctgcgcggc
    18601 accgcccaga accccgatgt ctacttccag gctcgcgaaa ccgtcaaccc ctactacacc
    18661 gcctgccctg ccaccgttca gcgtgccatg gatcgcttcg ccggcctcac gggtcgccag
    18721 tatcacctct ttgattacgc tggcgctccc gatgccgacc gcgtgatcat catcatgggc
    18781 tctggcgccg aaaccgccga agaaactgcc gccttcctgc agcagcaggg cgaaaaagtg
    18841 ggcgttctca cagttcacct ctatcgcccc ttctccattg agcacttgct caaggcactg
    18901 cctgccactg tcaagaaaat cgccgtcctc gaccgctgca aagagcccgg ctctgctggc
    18961 gaacccctgt accaggatgt cctcaccgct attgccgaag gtctcgatct gggcattgcc
    19021 cccttcaaga cttccccgcg cgttgtgggt ggtcgctatg gcctgtcttc caaagaattc
    19081 accccggcca tggtcaaagc catctttgaa gagctcaaga aagctgcccc caagaaccac
    19141 ttcaccatcg gtatcaacga tgatgttacc cattccagcc tgccctacga tcccgaattc
    19201 tcgatcgccg cagaagatgt ggtgcgctgt gtcttcttcg gcctgggtgc cgatggcacc
    19261 gtaggcgcca acaagaactc catcaagatc atcggtgaag aaaccgaatt tggcgctcag
    19321 ggctacttcg tctacgactc caagaaatct ggctcggtca ccatttcgca cctgcgcttt
    19381 ggcccgcgcc ccattcgcgc cccctacctc atcggtcagg gcgatgccaa ttttgtcggc
    19441 tgtcaccagt tctccttcct tgagcggctg gatatcctcc gctatgccgc tcctggcgcc
    19501 gtcttcctgc tcaacagcat ctacggcccc gaagaagtct gggactacct gccccgcgaa
    19561 gtccaggaag ccatcattac caagaagctg cgcttctacg tcattgatgc ctacgaagtt
    19621 gcccagaaga ccggcatggg tggccgcatt aacaccatca tgcagacctg cttcttcgcc
    19681 atcagcggtg tgctgccccg cgacgaagcg attgcccaga tcaagaaatc catcaagaag
    19741 acctacggca agcgtggtga agcggttgtc cagagcaact acgaagccgt agatgccacc
    19801 ctggcgcacc tctccgaagt caaagtacct gccaaggtca ccagcaccat cacccgccgc
    19861 ccggctgtcc cagccgaagc gcccgaattc gtgcgcgatg tcctcggcat gatgatcagc
    19921 tccgatggcg atgacctgcc cgtctctgcc atgcctgtgg atggcactta ccccaccggc
    19981 acaacccgct gggagaagcg taatattgcc ctcgaaatcc ccgtctggga atctgacctc
    20041 tgcatccagt gcggtaaatg cttctttgtc tgcccgcatg ccgccatccg cgccaaggtc
    20101 tacgaacccg ccctgctgga taatgctcct gcagccttca aatccaccga ggccaagttc
    20161 aaagaacttc ctggtctgcg ctacaccatc caggttgccc ccgaagactg caccggctgc
    20221 ggtctctgcg tggaagcctg ccccgccaaa gacaaggctc aggcaggccg caaggccatc
    20281 aatatggcgc ctcagccgcc catccgcgaa accgaagctg gaaactggac cttcttcctc
    20341 tctctgcccg atctggttga cagcccgctc aatcccggca tggtcaaaaa cgtccagctc
    20401 ctcgagccca tgttcgagtt ctcgggcgcc tgctctggct gcggcgaaac cccctacatc
    20461 aagctggtca cccagctctt tggtgaccgc atggttgtgg ctaatgccac cggttgttcc
    20521 tccatctatg gcggcaacct gcccaccacc ccctggtcca ccaacggcga aggtcgcggc
    20581 ccggcctggt ccaactcgtt gtttgaagac aacgccgagt ttggcctggg tatgcgcctg
    20641 accctcgaca agcaaaccga atttgcccgc gaactgattg ttgaactggc ggccgaaatc
    20701 ggcgaaaccc tgaccaccga actgctcaat gccgatcaaa ccaccgacgc cggcgtccgt
    20761 gcccagcgcg cccgcgtgga agccctgcgc agcaagctgg cgggcagcaa gaacccccgc
    20821 gcccgcttcc tgtctgaact ggccgatgcc ctggtccgca agagcgtttg gatcatcggc
    20881 ggcgacggct gggcgtatga cattggttac ggcggcctcg atcacgtcat ggcctccgga
    20941 cgcaatgtca atatcctggt cctcgatacc gaagtttact ccaacacggg cggtcaggct
    21001 tccaagtcca ccccacgcgc agcagttgcc aaattcgccg ccaacggtaa gggcctgccc
    21061 aagaaagacc tcggcctgat tgccatgtcc tatggctatg tctatgttgc caaggttgcc
    21121 atgggcgcca acgatgccca gaccctcaag gccttcctcg aggcagacgc ctacgatggc
    21181 ccgtctctga tcattgccta cagccactgt attgcgcacg gtattgacat gcgcaagggt
    21241 cttgaccagc agcgccttgc ggctcagtcc ggcatctggc cgatgtaccg ctacaatccc
    21301 gccctcatcg atcaggggca gaaccccctg acgattgaca gcaaagaacc caccgtttcg
    21361 gtgcaggaat acgcctacaa cgaaacccgc taccgcaccc tcatccaaac cgacgaagcc
    21421 cgcgccgaag cgctgatgct ccaggctaaa gaagacgtga cccgccgctg ggaatattac
    21481 cgccagttgg ccgccatgca ctacaagccc gacgagcagg cctaacccct ttcaatcgtt
    21541 gcagcccctg gccttcctga ccgggggctg cccctaagtc ttgaacggag gataatgatg
    21601 gttgatttga cgacaacata cctgggtttg acactcaaaa acccgctggt ggcctcggcc
    21661 tcgcctttat ccaaaaaggt agataccgtc cgccgcctgg aagatgccgg catcagcgct
    21721 gtggtgatgt actcgctctt tgaggaacag atttcccacg aaagccacgc gctggaccat
    21781 ttcctcagcc acggctccga atcttttgct gaggccctca cctacttccc cgaccttgac
    21841 cactacaaca tcggccccga tgagtatctt aatctcatcc gcaagctcaa gcagagcgtc
    21901 tccatcccgg ttgtcggcag cctcaatggc gtctccgctg gcggctgggt ggaccacgcc
    21961 cgcatgattg agcaggctgg cgccgatgcc ctcgaactga acatctacta cctgcccacc
    22021 gatgccgccc tgacagcggc cgaactggag caggcctatg tggacctggt ggccgctgtg
    22081 cgcaagcaaa tccgcatccc gctggcggtt aagctcagcc cctacttcac cgcgctgccg
    22141 cattttgcca gcgccctggt cggtgcgggc gccaatgccc tggtgctttt caaccgcttc
    22201 taccagcccg acctggacat tgaaacgctt gaagttctgc ccaccctcga cctgtccacc
    22261 tctgccgagc tgcgcctgcc ccttcgctgg acggcgctgt tatatggccg catcaaggcc
    22321 gacctggcgc tgaccaccgg tgtgcatacc gccaccgatg tcgtcaaagc aaccatggcg
    22381 ggcgccaatg tcaccatgct cgcgtctgag ttgattgccc acggcccggc ccgcgctgcc
    22441 gaaattgccg ccgacctgga gcaatggctg gtcaagtacg aatacacctc catcaagcag
    22501 atgcgcggca gcatgagcca gcaaaacgtc gccgaacccg cggcttttga gcgcgccaac
    22561 tatatgaagg ccctgcagtc cttcgataac cgcctgcgtt agtcttgctg ctgtcttgac
    22621 ctaaaagata accgccccga acattgtcgg ggcggttgat tttgataaca gcaggctgaa
    22681 acctaggctt ccaggggaag gttttcgttg ggtttcttgg atcgatagac aattcgcccg
    22741 cgggtcaaat catagggcga catttctaca cgcacccggt caccaagcaa aatgcgaatg
    22801 taatacttgc gcattctgcc cgagaggtac gccaaaacga catgcccatt atccaggcga
    22861 acccggaact gtgtggaggg cagggcctct acaattgttc cttcaacctg aatcttatct
    22921 tcttcttttg gcatacagcc tccttccaag ctcagggcaa aagccccggg cttaatccat
    22981 cgtccggcct acctggcggc gcttaatgcg gcgaatagcc tttttcttct ccacgcggcg
    23041 cagctcgctc tttgagacga accagcgctt gcggcgaact gtgctcaata caccactttt
    23101 cacaaccttt ttgcgaaagc gcttgagcag ttgatcctgc gattcatttg aacgcaacat
    23161 aacagagggc aatttattca cctcccttct ttcaagggaa tgggcaccag ggcctaaaaa
    23221 actcgactgc attcaggctg ccagccgagt ttgtgtcagt tatcctatca caattcgggc
    23281 ttgaagctaa tctgatcgca gcactctcca cataaataaa agaaagtaca tcatcctgtg
    23341 acaattattg gcacaagaac ggtgcaaatt atacccctaa ctgcccaaaa atgcaattat
    23401 gagtccggtt tgccgctcct ataaaaacag ctcttccttg cccgccagct tatcctgaat
    23461 ccgctcgatc acctcatcca gcgccttgct gtgatgcaca aaatccaggt catcggtgcg
    23521 gatggtcagc accgggcaaa gatcaaacgc ttgcatccat tcatcataaa gcgattccag
    23581 cagcgtcagg taatcggctg agatgctgct ctcaatcgcg cgcccgcgtc ggtggatgcg
    23641 cttcatcagc acctctaccg gcgccttcag gtgaatcagc agcgaaggcg gcggcagctt
    23701 cttgaccacc aggtcaaaaa gctgccggta ggttgcataa tcacgctcgc tcaggttgcc
    23761 cataatttgc agcgcccgcg cgaagataaa cgcatcttcg tagatactgc gatcaataat
    23821 cgccgggcgc gggtcggcgg ccagttccat gtgctgctca gcccggtgcc ccaggaagaa
    23881 gacctgcaaa tgaaaagacc aggcttgcat gtcggcgtaa aaatcgggca ggtaggggtt
    23941 gtctgccacc gattcgtaag ccgtgcgcca gcccatccgc tcacccagcc gctcggtcag
    24001 cgaggtctt
//
LOCUS       DAOPVS010000019        22953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2236_length_22953_cov_57.656389, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000019 DAOPVS010000000
VERSION     DAOPVS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 22953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22953
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2236_length_22953_cov_57.656389"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKL11_03410"
     CDS             complement(<1..377)
                     /locus_tag="PKL11_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNZ12680.1"
                     /translation="MRRILLTTLLILGFLLLALPAVQPARAQAPSAYDLIAAVNELRA
                     ANGLPAYEVAADLMGYAQSHSDYMASSGNITHTRADGSMPHDYGFIENIAGGTNLSIQ
                     TTIYEFWADALHWNTMVGVSSGTV"
     gene            complement(431..850)
                     /locus_tag="PKL11_03415"
     CDS             complement(431..850)
                     /locus_tag="PKL11_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNZ12681.1"
                     /translation="MELSVKGWMKDLVVFVEPEATVMEALSLMRHRYVNSLIAKKTAK
                     HPEYGIITSTDICDEIVAKGANPATTKVHEIMNTPLISVKPTDTIQECAAKMMANRIH
                     HLPVVDDKGNPTGMISATDFLVVAEAMGANFTERTLS"
     regulatory      980..1051
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1086..1556
                     /gene="crcB"
                     /locus_tag="PKL11_03420"
     CDS             1086..1556
                     /gene="crcB"
                     /locus_tag="PKL11_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNZ12682.1"
                     /translation="MKTRISLAQPMEKILLISAGAILGANLRYWLSLWAAQKWGADFP
                     FGTLIINLTGSFLIGLFLTLAAERFLVDPRWRLLIAVGFLGAYTTFSTYAFESYNLLA
                     AGRLMPALLNLLGSSLSGVLAVGLGIYAGKLMAGWTEWTAIAFSKFWQAWTQLG"
     gene            complement(1553..2839)
                     /locus_tag="PKL11_03425"
     CDS             complement(1553..2839)
                     /locus_tag="PKL11_03425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ12683.1"
                     /translation="MKKATPIIAWNSLPGPEDITRVELPNGITILTRSNFESPSVVIN
                     GYLPSGSLFDPPEKLGLSYFTALALMRGAGKRNFQEIFNSLETVGASLGFSASVHNTT
                     FGGRSLAEDLPLMLGVLADCLRTPTFPPDQIERLRAQLLTGLALRAQDTADMASITFD
                     AAVFAGHPYGNPEDGYVETVQPITRADLVDFHKRRYGPRGAVIVVVGAVTADHVIEQI
                     RKVLGDWQNPEQTDPPALPAIAPLTQTIRKHVPLAGKSQNDLVIGTLGPKRCATDYLA
                     ASLGNSVLGQFGMMGRIGEVVREQAGLAYYASTSLNAWIESGSWEVSAGVNPANTQRA
                     IDLIRSEIERFVREPVSAEELENSQANYIGRLPLSLESNAGVAGAILNLERFQLGLDY
                     YLRYPERVQRVTVEAVLEAAQHYLDPDKLAIISAGV"
     gene            complement(2836..4137)
                     /locus_tag="PKL11_03430"
     CDS             complement(2836..4137)
                     /locus_tag="PKL11_03430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ12684.1"
                     /translation="MPMPDAHHQVRLSNGLLIQLKEIHTAPIISHWVWYRVGSRYEQP
                     GKTGISHWVEHMQFKGTPRFPSGVLDKAISREGGYWNAFTYLDWTTYFETMPAHAIDL
                     ALESEADRMFNSIYTADEVESERTVVISEREGNENEPLFRLGEAMQGAAFEAHPYRNE
                     IIGSMDDLHAITRDDLYQHYRAHYAPNNAVVAIAGDFETETMLHRLERIYGGLAPAQV
                     PPPPQLVEPEPTAEKRVEVSGPGETTFLQLAYRAPAAREADFFAFTVLDSLLTGPTSL
                     NMFGGGGISNKTSRLYRALVERELAVGVGGSLQATYDPFTYDMTITVHPDQTPEVVLQ
                     AVDAEIRRLQQAPVKPDEIARAIKQARALFAYGSENITNQAFWLGYAEMFDSYHWFLN
                     YMERLAEVTPEDVLRVAQTCLQPTRRVVGIYRPTGAEEPNP"
     gene            complement(4138..4809)
                     /locus_tag="PKL11_03435"
     CDS             complement(4138..4809)
                     /locus_tag="PKL11_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12685.1"
                     /translation="MSVQIPSQEDEPRIEAAAEAPATRRWRPRGIGPAFWTLASIFSL
                     IVNVILIVVVLVLARNLFALKALVGDGLITPLYQNFVMMDEASIKRTIVVDSSVPAQF
                     DLELDTDTMVTLTEDTYIEDATVTVYTGGLYIDAASADIILPAGAELPIHLAMTVPVD
                     QTIPVNLDVEVDIPLNETELHTPFVGLQNVVAPYHEILSEMPSTWEQAICGPTPSKFC
                     QAIIP"
     gene            5024..>5454
                     /locus_tag="PKL11_03440"
     CDS             5024..>5454
                     /locus_tag="PKL11_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNZ12686.1"
                     /translation="MAEQPWQYRVAVIGAGPAGLFAARELVQQGVQVALINRDIKPGG
                     LAEYGIYPDKYKIKNGLRAQFHQILALDGLAYYGNLTVGDPGDLTLNQLRRLGFQALL
                     VTVGAQGTKWLGLPGEDLPGVYHAKDLVYHYNRLPPFSRAR"
     assembly_gap    5455..5554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5555..6510
                     /locus_tag="PKL11_03445"
     CDS             <5555..6510
                     /locus_tag="PKL11_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12687.1"
                     /translation="KDLVYHYNRLPPFSRARFNLGQRVIIVGVGNVMTDIAHYLISHC
                     SVTEVTALARRGPAEVKFDKKELELIGANLDLPAYDAEIARCAPLMRSLGQDPDAARD
                     FVHTAVASAAPSSGCARFRLHFLVSPVAIYAGPDRRVGSLEVEDNTLVMSGSQTKAQS
                     LGTRRQLEADTIIFAIGDRVDARLGLPIQGAEFVKNPAPRFPVEEQSYEAFDPVAQQP
                     IADVFVAGWARHASQGLVGIARRDGVNGARAVMQYLHTLPPLADLSPLAAGLSVSGKC
                     LVTQADLARLESAEQAEAARLGLPDFKFDTNEAMLEVINQT"
     gene            6719..7702
                     /locus_tag="PKL11_03450"
     CDS             6719..7702
                     /locus_tag="PKL11_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNZ12688.1"
                     /translation="MDLTKETRLEMYWMLLLSRRLDERAWVLHRQGKIAFHISGIGQE
                     AAQVGAAFALHRGEDWVTPYYRDLALMLSLGYTPREFVLSLMGKAEEPSSGGRQMPSH
                     WSLRRANAVSHSAPVATQACHAAGIGLAMKMRGEQKVVLTTIGEGSTSQGEWYEAVNW
                     AAVHQLPVIFMVENNQYAISVPQHLQMAVRSSADKACGLGLEGITVDGTDMLAVYEAV
                     DRAAQKARAGEGPTVIEARMYRITPHSSDDDDRTYRSREEVEENKKRDPIIVNRAHLE
                     AAGILTPAMVDEMEAKAKAIVDDAVDYATKAPYPAVEAGSYPVYAEEVRHG"
     gene            7695..8678
                     /locus_tag="PKL11_03455"
     CDS             7695..8678
                     /locus_tag="PKL11_03455"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNZ12689.1"
                     /translation="MAEITLVEAIRQAMDEEMARDERVFIVGEDVGVRGGVFRATVGL
                     FDKYGAARVIDSPLAELSIVGVGIGAALYGQRPICEIQFSDFIFPAYNQIVSEAAKMC
                     YRSNGEWTVPMVIRAPYGGGIGGGLYHSQSPEAMFAQIPGLKVVIPSNPHDAKGLLKA
                     AVRDPNPVIFFEPKKGYRLIKGEVPDDDYITPIGPAKISRSGSDVSVFAYGMMHHYAL
                     QAAEKVAAEGIDAEVVDLRTLYPVDRETILASVRRTGKALIVHEANLTGGYGAEIAAT
                     IVEGAFSDLDAPVRRLAGPDVPGVPFSHPLQEWFMPNVEKIAESIRQLSKW"
     gene            8692..10029
                     /locus_tag="PKL11_03460"
     CDS             8692..10029
                     /locus_tag="PKL11_03460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNZ12690.1"
                     /translation="MATQVIMPQLGESVVEGTVTKWLKKEGEPIQEFESLLEVNTDKV
                     DSEIPSPAGGTLLKILVPEGTTVRAGTVIAWIGQPGEVLPDSPAPVDLHAAPAAQPAA
                     ETKPTASVATGRDRDLGFISPVVARLAREQNVDLYQVKGTGEGGRITKKDVLAYLASG
                     GAAPKTAPEPAAWETPGEGDLFRPTELQFAGGAPGTTPAPKPAASPAQPLPRPDLSPA
                     PGDRLVPLSTMRKAIADHMVMSKRTSPHVSTIMEADLSRVIAHRQTNKAEFERSGVNL
                     TFTAYFVAAAAAALRAYPLVNSSWTDQGVLIHGAINIGMATALGEEGLIVPVIKQADN
                     LSLLGLARSINDLAARARAHQLRPDEVKDGTFTITNHGIGGSLFAMPVISQPQCAILG
                     VGAIKKRPVVVEGDAIAVRSMVYLSLTFDHRILDGSSGDGFLAKIVEVLEQWT"
     gene            10020..10649
                     /locus_tag="PKL11_03465"
     CDS             10020..10649
                     /locus_tag="PKL11_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12691.1"
                     /translation="MDVIRIDPLVSQIQSRFGWKISGQWNNAALTAILQAGDQIASYV
                     HSLNQRDGDAIVRRHFPAQFIHSDWLGSLWFLKGKSFVFPRRVVRLAPNFEIYQHPDW
                     HVVHELGHVLDNAVRRFFPATFLGGGLADRMIRDTGGRPHRCGLRFVPRADYIERCNL
                     MEHWEPIKSYGNTCVAEDFAETFVWTLFSSYRVPPRRLDWMRAFLKSLE"
     gene            10675..12072
                     /gene="lpdA"
                     /locus_tag="PKL11_03470"
     CDS             10675..12072
                     /gene="lpdA"
                     /locus_tag="PKL11_03470"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNZ12692.1"
                     /translation="MAEYDVVVIGAGPGGYVAAIRAAQLGLKAAIVDKQWLGGVCLNV
                     GCVPSKALLRNAEIAHILRERGKDFGFSMDNLQLDYSAAVKRSRQVSGRLTKGVGLLM
                     RKNNIDVVMGTARLTARDTVTVQDAEGKTQELKARNIIIATGAHTAMIPGVQADGQKI
                     LTYLEAILQERLPASAIIIGAGAIGAEFATIWSSYGSAVTMVEMLPRILPLEDEEVSV
                     ELAKAFQKRGIKIHTGTRVQSIETTETGVRVTVSAGEQTQVLEAEQALVAIGFKPNTA
                     DLGLEELGVKLTERKFIQIDEHMATNVPGIFAIGDVTGQLLLAHVASAQGILCAEHIA
                     GHQTTPLDYRMMPRATYSQPQVASFGLTEAQAREQGHEIKVGRFPFQPNGKALGLGDY
                     AGFAKIISDAKYGEILGAHLIGPEVSELLPELTLAQRMELTAEEIARNVHAHPTLSEV
                     LMEAAHGVDGQPIHI"
     gene            12177..13001
                     /locus_tag="PKL11_03475"
     CDS             12177..13001
                     /locus_tag="PKL11_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12693.1"
                     /translation="MPAYSRSSKRPLGQITRGKTAPNRLRRVDNFICQYDPGLLRLRA
                     LENCFYVDLGYGAEPFTTLESAARLRRFNPHLPVLGIEIDPERVSRALPFADEQTLFR
                     LGGFNVPLQPGETVRLIRAMNVLRQYEEAEFLSAVAQMGHSLIPGGLLLEGTSDPFGR
                     IMVINLIRRTLEGSLMMEGLLFSTNFRWGFEPGVFQPVLPKNLIHRMIPGEMIYDFME
                     AWKQAARLTIGIRPLGLRQWFVASARALTEMGWSIDPRRRFLQQGYLLWKRSIPLD"
     gene            13174..17208
                     /locus_tag="PKL11_03480"
     CDS             13174..17208
                     /locus_tag="PKL11_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12694.1"
                     /translation="MLQKIVRILGGDPQGRELKKYIEIVGQINALEKPYEALSDEALT
                     AKTAEFRSRVAAGETLDDLLPEAFAAVREASKRTIGLRHYDVQLIGGMALHNGRISEM
                     RTGEGKTLVATLPVYLNALTGRGVHLVTVNDYLARRDARWMAPIYNLLGLTIGVLQMA
                     SRTENGRKAFVVDLTKTSPFEDQDKIRLVDRGEAYKADIVYGTNSEFGFDFLRDNLTM
                     RLSERVQRDHYFAIVDEVDNILIDEARTPLIISGPASDDTEWYVKMAQVVRSLRPEDI
                     EVNERDRNAILTEVGEAHVEEILGIPLRDPDRPEDINPEQARLWGYLEQSLKAQYLFR
                     RNKDYIVQSGKVVIVDEFTGRLMPGRRWSEGLHQAVEAKEGVRVEPENVTYATITIQN
                     YFRMYEKLCGMTGTAKTEAEEFAKIYKLDVFQIPTNLEYQAMGGPAARFIEVTAKDEQ
                     GYKHQYYARRDDPKQAPIFFRRKDYPDVIYRSEEAKLRAITTEALRYHIQGRPQLIGT
                     TSVEHSERLSDRFSAEAIRRLAQVMLLRDFWLTKNNRQPNEQSIPELAFLNKPLMELN
                     PGEMRQSARSLGMSSLNPEDADNATRLLQLLELTPNDQPRLLKVLQAGITHQVLNARK
                     HDEESKIIARAGGFGAVTIATNMAGRGVDIRLGGELADGIRPDVLRVLRRAESDLYNL
                     TDEELYRSLQEVPPDQYGIYAESVQTFQQFMEEMARVRALGGLHVIGSERHEARRIDN
                     QLRGRAARQGDPGSSRFYLSLEDDLMRLFGGQQVENLVSRFNLDENIPIEVGLVGRMV
                     EQSQERVEGANFDMRKHLLEYDDVLNSQRHRIYAQRDRVFTKADLSDDVAEMLQAEVN
                     KRVEAGLSDPEGPWKLLAYLEEIQPPIQHEGVSYPTYTHQLLVEEIQRRLRASGADLL
                     NVLVNLAAEALDAERDHLLNSVRQLLEKLEETYKQQLSERTDLLDTFMEGLKDREDDQ
                     PVRAQTVLDELSAALRLPIKLTPDQLRALPEGGRPVVTAIRQQIADNLIILTITRAVI
                     TLERRVGDLGLRPAQFQGLEWDEVADALFTAVDSALIARRDRLVGAEGQIARDLAPQL
                     ERLGDSASLPNYLLGLLIFTAQGAQQDFDARTHRRIVRRVVRLTYIFLAAHLLGKRST
                     EDVSESVFNHLKGAEAAMQIVWGRAEWNRLRQSEATLGQLDPALQDLLSAALGTDRLE
                     KLAATPLAEFEPADRDAAVNVLGARLQNESYRELLLRVISNNWVDYLTRIEALRVSIT
                     MESYAQRDPLVQYKGRASELFSELLAEIRSGVATSMFTYRPTRGAAAAVDRTGAPEPA
                     ALPASAPAEQQPVSAERAKKRKRH"
     gene            17382..>18675
                     /locus_tag="PKL11_03485"
     CDS             17382..>18675
                     /locus_tag="PKL11_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNZ12695.1"
                     /translation="MDQNNAQLEQGETPETNPVENHTNMASLLEEEGLGIDFPQAGEI
                     RKGVVASITPGQILVSVGTKSEGVISGKEFELIPPEELAALAVGQEIPVYVINPEDQN
                     GNVVLSYTRAREEVSWEQVDELLRGKECIHSTIVGYNKGGLIVPVVGLRGFVPASQIS
                     LSRRSRLSGDSPEQMWGKMINEEIDVCVIEVDRERRRLILSERAASTETRETLKERVI
                     EELQEGEVRNGRVTSLADFGAFVNISGADGLVHLSEISWDRIQHPSEVLRVGQEVKVK
                     IISVDREKKRIGLSIRQLQSDPWSQRVSHLQIGQLVEGTITRLTKFGAFARIGDDLEG
                     LIHISEISDKRIEHPKEVLKEGDVVTLRVIKIDVENHRIGLSLRKVESMAFADMDWQS
                     IEGIIADAGEIAAPAEDIIADAGEPEAPADEQPDTSEQA"
     assembly_gap    18676..18775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18883..19980
                     /locus_tag="PKL11_03490"
     CDS             18883..19980
                     /locus_tag="PKL11_03490"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNZ12696.1"
                     /translation="MPILIDSHEDLAYNILTFGRDYRRSAAETRRLEVGQRVLEATGN
                     ALLGWPEYQRGQVALVCGTIFVGPRRYLKHDWETQTYDDYAGARRLMRAQVDVYRRLA
                     EESPDMFTLVRTRSELDAVLAPWDAAPANPPQVTHPTGLVILMESAEGLAEPEEVEEW
                     WELGVRIIGPVWGGSRWCGGTVEPGPFRPEGFRLLDVMADLGFTLDISHMSDESAVQA
                     LEYYRGRVIASHANIRALIPGDYSPRQLTEAALRRLIERDGVMGFIPFNRFLKADFVN
                     SDPRETVTLDHYLKHIDTLCQMAGNAHHAAIGTDFDGGFGWPWVPLELDTIADLQQIA
                     PRLQSWGYSDADVAAILGGNWRRLLEETLPR"
     gene            19977..20465
                     /locus_tag="PKL11_03495"
     CDS             19977..20465
                     /locus_tag="PKL11_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12697.1"
                     /translation="MTAAVNADNRQDTIPELPYSRRRIRWGLGLTLVGFVVFLLGVRP
                     ALFGADRSPVVGFVQIAVFLIGLAFMCIGGYISMMALWKKAEPSIAADIGLRLVATGY
                     VIAVFAGMADVFGLGSHPLPEVPYFGLWQARGVELGQALIAIGFLLLLPLRRLFHRPE
                     HH"
     gene            20533..21747
                     /locus_tag="PKL11_03500"
     CDS             20533..21747
                     /locus_tag="PKL11_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNZ12698.1"
                     /translation="MNSQSTSDETLPADLQTENLDSPQPKNSWRQAWEKIVRLGLGEI
                     ALRTGTAVVSLALILVVIWVMATFFLKGDASNNLAGAAVLAAPGTVNAQAPTPTAQPP
                     AFVIPQSTAHVNGITRLAQLHTLLPSRPRYDVITYTVLEGDTIFGIAEKFGLKPQSIL
                     LGNRDLLGDNPHYIQPGMVLNILPSDGIYYRWNAGDGLNGVSSYYKVTPEVIINWPGN
                     HLDPATLGDWSNPNIADGTMLFVPGGQAEFITWSAPVITRDNPGVARLFGPGYCGVIT
                     TGAVGDGVFVSPTVLNYLSGYDYTPDTNHFGLDFAGQMGTPIYAIDDGVVVYSGWNDH
                     GYGYVMVIDHGNGWQSLYAHLSEIYFGCGQSVFSGDTIAAMGSTGRSTGPHLHFELRY
                     NGNFVNPWNVLP"
     gene            21757..22602
                     /gene="amrB"
                     /locus_tag="PKL11_03505"
     CDS             21757..22602
                     /gene="amrB"
                     /locus_tag="PKL11_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNZ12699.1"
                     /translation="MAAHSDLRPSPIAGTWYSGDPQRLGQQVDAYLDQAVLPPLSGRV
                     VGLVAPHAGHRYSGRTAGHAFAAVRGQSFDQVVVLSPLHDFHPAPLLTTAHRAYSTPL
                     GPVPVDVPAVEALDAALAVQNLRLTPVANDREHSLEIELPFLQRALSGDFRLIPIMVR
                     SRATSVCRALGLALAELLRDHSALLVASTDLSHFYPLHTADALDAEMLRRIAAFSPED
                     VLSAEETGAGFACGAGPVAVMLWAARALGANTVTILHHSTSAEETGDTHSVVGYGAAV
                     ALGQG"
     gene            22606..>22953
                     /locus_tag="PKL11_03510"
     CDS             22606..>22953
                     /locus_tag="PKL11_03510"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12700.1"
                     /translation="MAHFVQIAVNVPLASGVFDYHLPPELVDSIQPGSLVVVPFGQQT
                     VQGLVLRSVAEPQVQKTRPVESLLDPQPVLTPPQMALAAWLSEETLAPLALCLHLMLP
                     PGLSQQADTLYSLH"
BASE COUNT         4575 a         7026 c         6239 g         4913 t
ORIGIN      
        1 accgttccgc tggacacgcc caccatggta ttccagtgca gtgcatcagc ccaaaattca
       61 taaatggtgg tctggatgga caggttggtg ccgccggcaa tattctcgat aaacccgtaa
      121 tcatgcggca tcgaaccatc cgcccgcgtg tgggtaatgt ttccgctgga ggccatataa
      181 tccgaatggc tttgcgcata gcccatcagg tcggcagcca cttcataggc tggcagaccg
      241 ttggcggcgc gcaattcatt cactgccgca atcaaatcat atgcgctggg cgcctgagcc
      301 cgcgccggct gcaccgctgg cagtgccaac aatagaaagc ccagaataag cagggtggta
      361 agaagtattc ggcgcatcgt ttttttatta taaccgatgc gccgtgagat tcaatccgtt
      421 aattatttgc ctagctcagg gtgcgctcag taaagtttgc ccccatcgct tctgccacca
      481 ccagaaaatc tgtcgccgag atcatccccg tgggattgcc tttatcatcc accactggca
      541 ggtgatgaat ccggttcgcc atcatcttcg ccgcacactc ttgaatcgta tcagtcggct
      601 tcaccgaaat caacggcgtg ttcataatct cgtgcacttt ggttgtagcc gggttagcac
      661 ccttggctac aatctcatca caaatatccg tggaagtgat aatcccatac tcaggatgct
      721 tggctgtttt cttcgcgatc aggctgttca cataacgatg ccgcatcagc gacaatgctt
      781 ccattaccgt cgcctcgggt tcaacaaaga ccaccaggtc tttcatccag cctttaacac
      841 tcagttccat gatggatttt ctctcctttg ggatatgttg gggcgcagca ctgcgctttg
      901 acatgctaag tatatgcaaa agagacctaa aaggcaagcc gggcagccaa tcacttgacg
      961 cctcccataa tccggataca attgcccggg cgatgaagct cgcccggtgc aaaccaaacc
     1021 gccctcccag gctgatagct tctgccccat tctcaggcag aggctttttt attgtcagaa
     1081 accgcatgaa aacaaggatc tcgctcgcac aacccatgga aaagatactg ctcatctcgg
     1141 cgggcgccat tctgggcgcc aatcttcgct actggctcag cctttgggcg gctcaaaaat
     1201 ggggtgccga ttttcccttt ggcaccttga tcattaacct taccggcagc tttttaattg
     1261 gcctcttcct caccctggct gctgaacgtt tcctggtaga tccgcgctgg cgcttactga
     1321 tagccgtggg cttcctggga gcctatacca ccttttccac ctatgccttt gaaagttaca
     1381 acttgctggc cgccggccgc ctgatgccgg ccctgctcaa tctgctgggc agcagccttt
     1441 ccggtgttct ggcggttggt ttgggcatct atgctggcaa attaatggcc ggctggacgg
     1501 aatggaccgc cattgcgttt tcaaaattct ggcaggcctg gacgcagttg ggctaaaccc
     1561 ccgcgctgat aatcgccagc ttatccgggt ccagataatg ctgggcggct tccagcaccg
     1621 cctccactgt cacgcgctgc acacgctccg ggtaacgcag ataataatcc aaacccagtt
     1681 gaaaccgctc caggttgagg atggcccctg ccacgcctgc attagattcc agcgacagcg
     1741 gcagccgccc aatataattg gcctggctgt tttccaattc ctcagctgag accggctcac
     1801 gcacaaaccg ctcaatttct gatcggatta aatcaattgc ccgctgtgta ttggccggat
     1861 ttacccccgc cgaaacctcc caggaccccg attcaatcca ggcgttcagg ctggtcgagg
     1921 cataataggc cagcccggct tgttcacgca ctacttcgcc aatccggccc atcatgccaa
     1981 actgtcccag cacgctgttg cccagagagg ctgccagata gtctgtggca catcgcttgg
     2041 gccccagggt gccaatcacc aggtcattct gactcttgcc agccagcgga acatgtttgc
     2101 gaatggtctg cgtcagcggc gcaattgccg gcagcgcagg cggatcggtt tgttcggggt
     2161 tttgccagtc tcccagcact ttacggatct gttcaatcac atggtcggct gtcactgctc
     2221 ccaccaccac aatcaccgcc ccgcgcggcc catagcgccg cttatgaaaa tctaccaggt
     2281 cggctcgtgt aatcggctgc acggtctcca cataaccatc ctccgggttg ccgtaggggt
     2341 gcccggcaaa gaccgccgcg tcaaacgtga tgcttgccat gtcggccgta tcctgagcgc
     2401 gcaatgccag cccggtcaac agttgcgccc gcagccgctc aatctggtca ggcggaaaag
     2461 tcggcgtgcg caggcagtct gccaacaccc ccagcatcag cggcaggtct tccgccaggg
     2521 atcgcccgcc aaaagtggtg ttatgcacac tcgcgctgaa acccaggctg gcgccaaccg
     2581 tctccagcga attgaaaatc tcttgaaagt tccgcttgcc cgcgccgcgc atcaatgcca
     2641 gtgccgtgaa ataggataag cccagctttt ctggcgggtc aaacaggctg ccgctgggca
     2701 aataaccatt gatgaccacc gacgggctct caaaattgct gcgcgtcaaa atcgtaatgc
     2761 cgttgggcag ttccacccgg gtaatatctt ccggccccgg cagcgaattc caggcaatga
     2821 tgggggtggc ctttttcatg ggttgggctc ctcagcaccg gttggacggt aaatgcccac
     2881 cactcgccgg gtgggctgca ggcaggtttg cgccacccgc agcacatctt cgggcgtcac
     2941 ctctgccagt cgttccatgt aattcaggaa ccagtgatac gaatcaaaca tctcggcata
     3001 tcccagccag aaagcctgat tggtgatgtt ctcgctgccg taggcaaaca gcgcccgggc
     3061 ctgtttgatg gcgcgggcaa tttcatccgg tttcaccggc gcttgctgca gccgtctaat
     3121 ttcagcatcc accgcctgca gcaccacttc gggggtctgg tcggggtgta cggtaatggt
     3181 catatcatag gtgaagggat cataggtagc ctgcaggctg ccgcccaccc ccaccgccag
     3241 ttcgcgctcc accagcgccc ggtacaaccg gctggtctta ttcgaaatgc ccccgccgcc
     3301 aaacatattc aggctggtgg ggccagtcag caggctgtcc agcacagtaa aggcaaagaa
     3361 atcggcctcc cgcgctgctg gcgcccgata agccagttgc aaaaaggtgg tttcgccggg
     3421 gccgctgact tccacgcgtt tttcggcggt tggctctggc tcaaccagtt gcggcggcgg
     3481 tggcacctgc gcaggcgcca gtccgccgta aatccgttcc aaccggtgca gcattgtctc
     3541 ggtctcaaaa tcgccggcaa tcgcaaccac ggcattgttg ggcgcataat gcgctcgata
     3601 atgctgatat aaatcatccc gcgtgatcgc gtgcagatca tccatgctgc caatgatttc
     3661 attgcgatag gggtgagcct caaacgccgc tccctgcatg gcctcgccca gccggaagag
     3721 gggctcgttt tcattcccct cacgctccga aatcaccacc gtgcgctctg attcaacctc
     3781 gtctgctgtg tagatgctgt tgaacatccg gtctgcttcc gattccagcg ccaggtcaat
     3841 tgcatgggct ggcatggtct caaaataggt cgtccagtcc aggtaggtga aggcgttcca
     3901 gtagccgcct tcccgtgaga tagccttatc cagcacgccg gaggggaacc ggggcgttcc
     3961 cttaaactgc atatgctcca cccagtgcga aatgccggtt ttgccgggct gttcatagcg
     4021 tgagccaacc cggtaccaca cccagtgact gatgatcggt gccgtgtgta tctctttgag
     4081 ctggatcagc agcccgttgg ataggcgaac ttggtgatgg gcgtctggca tgggcatcta
     4141 gggaatgatc gcctggcaga atttactcgg tgtaggcccg cagattgcct gttcccaggt
     4201 gctgggcatc tcgctcaaaa tttcatgata cggtgccaca acattctgca gccccacaaa
     4261 tggggtatgc agctcggtct cattcagtgg aatgtccact tccacatcca gattcaccgg
     4321 gatggtttga tccaccggca ccgtcatcgc caggtggatg ggcagctctg ctccggctgg
     4381 caggatgata tctgcgctgg cggcatcaat atacaacccg ccggtataaa ccgttaccgt
     4441 ggcatcctcg atataggtat cttctgtcag tgtgaccatt gtatcggtat ccagctccag
     4501 gtcaaactgc gccggtacgc tgctgtccac aacaatggtt cgcttgatgc tggcttcgtc
     4561 catcatcaca aaattctggt acagcggtgt aatcagtcca tcgcctacca gggctttcaa
     4621 cgcaaacagg ttgcgcgcca gcaccagcac caccacaatc aaaataacat taacaatcag
     4681 agaaaagatg gacgccagcg tccagaaagc cggtccaatc ccgcgagggc gccagcgacg
     4741 cgtcgccggt gcttcggctg ctgcctctat gcggggttcg tcttcttggc ttgggatttg
     4801 tacactcatt gaaactacct ccagagtatt gaatccgtgt cttcacggag taaaatacaa
     4861 gtatatacat gaatatagca tagacttgtg attttgaaaa acgaaacagg gtataattat
     4921 tgtattgtgc aataacaatc taattactct actttttacc cggtgatggg cctgcggttg
     4981 tttgtcagca ggctccatct gacagatcaa aggaggcgcg cgcatggcag aacaaccctg
     5041 gcaatatcgg gtcgcagtaa ttggcgccgg gccagccggg ttgtttgctg cgcgcgagct
     5101 ggtacagcag ggggttcagg ttgcactgat taatcgcgat atcaagcccg gtggtttggc
     5161 agagtacggc atctatcccg ataaatataa aatcaaaaat ggcctgcgag cgcagtttca
     5221 ccagatcctc gcgctggatg gcctcgcata ttacggcaat ctgacggtcg gcgatcccgg
     5281 cgatctcacc ctcaatcaac tgcgccggct gggttttcag gccttgctgg ttaccgttgg
     5341 cgctcagggc accaagtggc tgggtctgcc cggtgaagac ttgcccggtg tctatcacgc
     5401 caaagacctg gtctatcatt acaaccgtct gccgccgttc agccgggctc gcttnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccaaag acctggtcta tcattacaac
     5581 cgtctgccgc cgttcagccg ggctcgcttt aacctggggc agcgagtgat cattgtgggt
     5641 gttggcaacg tgatgactga cattgcccac tatctcatca gccattgcag cgtaactgaa
     5701 gtgactgccc tggcgcgccg cggcccggca gaggtcaagt ttgataaaaa agaacttgaa
     5761 ctgatcggcg ccaacctgga cctgcccgcc tacgatgccg aaattgctcg ctgcgcgcct
     5821 ttgatgcgct cgctggggca agaccccgat gccgcccgcg actttgtgca tacggctgtc
     5881 gcctctgccg caccctcgtc cgggtgtgcg cgctttcggc tccattttct ggtcagcccc
     5941 gtagccatct acgctggccc ggaccggcgg gtgggcagcc tggaagtgga agataatacc
     6001 ctggtgatgt cggggtcaca gaccaaagcc cagtctctgg gcacccgccg ccagcttgag
     6061 gctgatacta ttatctttgc cattggcgac cgcgtggatg cccgcctggg tttgccaatc
     6121 cagggtgctg agtttgtcaa aaacccggcg ccgcgctttc cggtggaaga gcaatcttac
     6181 gaggcgtttg atccagtcgc acagcagccc atcgccgatg tctttgtagc ggggtgggca
     6241 cgccatgcca gtcaggggct ggtcggcatc gcccgccggg atggcgtcaa cggcgcccgg
     6301 gcggtcatgc agtatttgca cacccttcca ccgctggcgg atttatcccc cttggcagcg
     6361 gggttgtccg tctcgggcaa gtgtctggtt acccaggcag atctggcccg cctggaatcc
     6421 gctgaacagg ctgaggctgc ccggctgggt ctgcccgatt tcaaattcga tacaaatgaa
     6481 gccatgctgg aggtcatcaa ccaaacctga acccccaaat acaactttta cgctgggaaa
     6541 cgatctaaat gatattggaa catgtagctg cctggcaagg agcagaacga gggttacgct
     6601 ttggcgtgtg gacttcagcc cgccagattg tgcaggcaag aacccgcctg ctgtatgaga
     6661 cataaattct gccgttgccc gagtgctaac tggaaaacga tgaaaaggag aacagggtat
     6721 ggatctgacg aaagaaacca gactggaaat gtactggatg ctgctgctgt ctcgccggtt
     6781 ggatgagcgc gcctgggtct tgcaccgcca gggcaagatc gccttccata tttccggcat
     6841 cggtcaggag gccgcccagg tgggcgcagc ctttgcgctg caccggggcg aagattgggt
     6901 caccccctac tatcgcgacc tggcgctgat gctcagcctg ggctacaccc cgcgtgaatt
     6961 tgtcctcagc ctgatgggta aagctgaaga acccagttcc ggcgggcgcc agatgcccag
     7021 ccactggagc ctgcgccgcg ctaacgcggt cagccattcg gctccggttg ccacccaggc
     7081 ctgccatgcc gccggaattg gcctggcaat gaaaatgcgc ggcgaacaaa aagttgttct
     7141 caccaccatt ggcgaaggtt ccacctctca gggcgaatgg tatgaggccg tcaactgggc
     7201 ggctgtgcac caactcccgg tgatttttat ggtcgagaac aaccagtacg ccatttcagt
     7261 tccccagcat ttacaaatgg ctgttcgcag ctctgcggat aaagcctgcg gcctcggcct
     7321 cgaaggcatc accgttgatg gtaccgatat gctggcggtg tatgaggcgg tcgatcgcgc
     7381 cgcccaaaag gcccgcgccg gtgaaggccc caccgtcatc gaagcccgca tgtaccgcat
     7441 taccccgcac tcctctgatg atgatgaccg cacctaccgc agccgtgaag aagtggagga
     7501 aaataaaaag cgcgacccca tcattgtcaa ccgtgctcac ctggaagcag cgggcattct
     7561 gacccccgcc atggtggacg aaatggaagc caaagccaaa gccattgtag atgatgccgt
     7621 ggattacgcc accaaagccc cttacccggc ggttgaagcc ggcagctacc ccgtttatgc
     7681 cgaggaggtg cgtcatggct gagattaccc tggttgaagc catccggcag gccatggacg
     7741 aagaaatggc tcgcgatgag cgtgtcttta tcgtgggtga agatgtcggc gtgcgcggcg
     7801 gcgtgttccg cgccactgtt ggcctctttg ataaatatgg cgctgcccgg gtgattgact
     7861 cgcctctggc agagctttcc attgtgggtg tgggcattgg cgctgccctg tatggccagc
     7921 gccccatctg cgagattcag ttctccgatt ttattttccc ggcctacaat cagatcgtca
     7981 gcgaagccgc caaaatgtgc taccgctcca atggcgaatg gaccgtcccc atggtcatcc
     8041 gtgcccctta cggcggcggc attggcggcg gcctctatca ttcacagagc cccgaagcca
     8101 tgtttgccca gattccaggc ctcaaggtcg tgatcccctc caaccctcac gatgccaaag
     8161 gcctgctcaa agccgccgtg cgagacccca accccgtgat cttctttgaa cccaagaagg
     8221 gctaccgcct cattaaaggc gaggtgccgg atgatgatta catcaccccg attggcccgg
     8281 ccaagatttc ccgctctggg tccgatgttt cggtctttgc ctacggcatg atgcaccact
     8341 acgccctcca agccgccgaa aaggttgcgg ctgaaggcat tgatgccgaa gtggtggatt
     8401 tgcgcaccct ctaccccgtg gatcgtgaaa ccatcctggc ctctgtgcgc cgcaccggca
     8461 aagccctgat cgtccacgag gccaacctga ccggcggcta cggcgccgaa attgccgcca
     8521 caattgtgga gggcgccttc tcagatctgg atgcccctgt ccgccgcctg gccggccccg
     8581 atgtcccggg tgtccccttc agccacccgc tgcaggaatg gttcatgccc aatgtggaaa
     8641 aaatcgccga atccattcgg cagttgagca agtggtaaaa aggatgatga tatggctaca
     8701 caggtaatca tgccccagtt gggtgagagt gtggtcgaag gcactgtcac caaatggctc
     8761 aaaaaagaag gtgaacccat ccaggaattt gaatccctgc tggaagttaa caccgataaa
     8821 gtcgattcag aaatccccag cccggcaggt ggcaccctgc tgaagattct ggtccccgaa
     8881 ggcaccaccg tccgcgctgg cacggtcatc gcctggattg gtcagcccgg cgaggtgctg
     8941 cctgattcgc ccgccccggt ggatctccac gccgcacccg ccgcccaacc agcggctgaa
     9001 actaaaccca ctgcgtctgt cgccaccggg cgagaccgtg atctgggctt tatttccccg
     9061 gttgtggccc gcctggcgcg tgagcagaat gtggacctgt accaggtgaa gggtaccggc
     9121 gagggaggcc gcatcaccaa gaaagatgtc ctggcgtacc tggcatccgg aggggctgca
     9181 cccaaaactg cacccgaacc cgccgcctgg gaaacccccg gcgaaggtga ccttttccgc
     9241 cccaccgaat tgcagtttgc cggaggcgcc cccggcacga ccccggcgcc caaaccggca
     9301 gccagtcctg cccaaccgct cccgcgccca gacctcagcc ctgctcccgg cgaccgtctt
     9361 gtgccgctca gcaccatgcg caaagccatt gcagaccaca tggtcatgag caaacgcacc
     9421 tcgccccatg tcagcaccat catggaggcc gacctcagcc gcgtgattgc acaccgccag
     9481 accaacaaag ccgagtttga gcgcagcggc gtcaatttga ccttcaccgc ctactttgtg
     9541 gcggcagcgg ctgccgcact gcgggcttac cccctcgtca actcctcctg gaccgatcag
     9601 ggcgtcttaa tccatggcgc aatcaatatt ggcatggcca ccgccctggg cgaagaaggg
     9661 ttgattgtac cggtcatcaa gcaggccgat aacctctctc tgctcgggct tgcgcgttca
     9721 atcaacgatc tggcagcgcg ggctcgcgcc catcagctgc ggcccgatga agtcaaagac
     9781 ggcaccttta caattaccaa tcacggcatt ggcggctcat tgtttgccat gccggtgatt
     9841 agccagcccc agtgcgccat cctcggcgtt ggcgccatca agaaacgccc cgtggtcgtt
     9901 gaaggggatg ccatcgccgt gcgctcgatg gtctatctct ccctgacatt tgaccaccgt
     9961 attctggatg gctcatccgg cgacggcttc ctggcgaaaa ttgtcgaagt attggaacaa
    10021 tggacgtaat ccgaattgac ccgcttgtct cacaaatcca gtcccgcttt ggttggaaaa
    10081 tcagcggtca gtggaacaac gccgctctga cggcaatcct gcaggctggc gatcaaatcg
    10141 ccagctacgt ccattcactc aaccagcggg atggtgatgc catcgtccgg cggcattttc
    10201 ccgcccaatt catccattcg gattggctgg gcagcctctg gttcctcaaa ggcaaaagtt
    10261 ttgtcttccc gcgccgggtg gtccggctgg cccccaactt tgagatctac caacaccccg
    10321 actggcacgt ggttcacgaa ctggggcatg tgctggataa tgctgtccgg cgcttctttc
    10381 cagcaacgtt tttgggcggc ggcctggccg atcgcatgat ccgggatacc ggagggcgcc
    10441 cccaccgctg cggactgcgc tttgtccccc gtgccgatta tatcgagcgc tgcaacctca
    10501 tggaacactg ggagccgatc aaatcctacg gcaacacctg cgtggccgaa gactttgccg
    10561 agacctttgt ctggactctg ttcagttcgt acagggttcc gccccgccgt ttggactgga
    10621 tgcgggcatt tctcaagagt ttagagtaaa attgttgcgg aggataacat aaacatggca
    10681 gaatatgatg tcgttgtcat cggcgctggt ccgggcggtt atgttgccgc catccgcgcc
    10741 gctcagttgg gtctcaaagc cgccattgtc gataagcagt ggctgggtgg cgtttgtctg
    10801 aatgtgggct gcgtgccttc caaggcgctg ctgcgcaatg ctgaaattgc ccacattctg
    10861 cgcgagcgcg gcaaagattt tggctttagc atggataacc tgcagttgga ctattccgcc
    10921 gcagtcaaac gcagtcgcca ggtttcgggc cgcctgacca aaggtgtggg cctgttgatg
    10981 cgcaagaata acattgatgt tgtgatgggc acagcccgcc tgaccgcgcg cgataccgtg
    11041 accgtgcagg atgccgaggg caaaacccag gaactcaaag cgcgcaatat cattattgcc
    11101 accggcgcgc ataccgccat gatccccggg gttcaggcag atggtcaaaa gattctcacc
    11161 tatctcgaag ccattctgca ggaacgcttg ccggcttcag ctatcatcat tggcgcgggc
    11221 gccatcggtg ccgagtttgc caccatctgg agctcctatg gctctgccgt gacgatggtg
    11281 gaaatgctgc cccgcatcct gccgctggaa gatgaagagg tcagtgtgga actggccaag
    11341 gccttccaga aacgcggcat caagattcac accggcacac gcgtccagtc tatcgaaacc
    11401 actgagaccg gcgttcgtgt gaccgtcagc gcgggtgagc aaacccaggt gctcgaggcc
    11461 gaacaggcgc tggtggccat tggcttcaaa cccaataccg ccgatctggg tctggaagaa
    11521 ctgggcgtca aactgaccga acgcaagttc atccagattg acgaacacat ggcgaccaat
    11581 gtgccgggca tttttgccat tggtgatgtg acagggcagc tcctgctggc gcatgtcgcc
    11641 tctgcccagg gcattctctg cgccgagcat atcgccgggc atcagaccac cccgctcgat
    11701 taccgcatga tgccccgcgc cacctactct caaccccagg tggcctcctt tggcctcacc
    11761 gaagcccagg ctcgcgaaca gggccatgag atcaaggtcg ggcgcttccc cttccaaccc
    11821 aacggtaaag ccctgggcct gggtgattat gccgggttcg ccaaaatcat cagcgatgcc
    11881 aagtatggcg aaatcctggg cgcccacctc attggccccg aggtatctga actgctccct
    11941 gaattgaccc tggcccagcg catggaactg actgccgagg aaattgcccg caacgtccat
    12001 gcccacccca ccctcagtga agttctgatg gaagccgccc acggtgtgga cggacagccc
    12061 atccacatct aatccaaccc aaccgcgtcg tccctcttga cctgtgaaga gggacgacgc
    12121 cgttcacatg cgcaggtggg gttggctgta acctcgcctt ttaatttttt tccactgtgc
    12181 ctgcctattc tcgttcttct aaacgccccc tggggcaaat tacccgcggt aaaaccgccc
    12241 ccaaccgcct gcgccgggta gataacttta tctgccagta tgacccgggg ctgctccgcc
    12301 tgcgcgccct cgaaaactgc ttctatgttg atctggggta tggggctgag cccttcacca
    12361 ccctcgaatc cgctgcccgc ctgcgccgat ttaatccgca cctgcctgtc ctcggcatag
    12421 aaattgaccc cgaacgcgtg tctcgtgccc tgcccttcgc cgatgagcag actctcttcc
    12481 ggttgggcgg cttcaatgtc ccgctgcagc ccggcgagac ggttcgcctc atccgggcga
    12541 tgaatgtctt gcgccagtat gaagaagccg aatttctctc cgctgtcgct caaatggggc
    12601 acagcctgat ccccggtggg ctgctgctgg aaggcacctc ggatccgttt ggtcgcatca
    12661 tggtcatcaa cctgatccgc cgcacgctgg agggtagttt gatgatggaa ggtctgctct
    12721 tcagcaccaa cttccgctgg ggttttgagc cgggcgtttt tcagcctgtg ctgcccaaaa
    12781 atctgattca ccgcatgatc cccggcgaaa tgatctacga ttttatggag gcctggaaac
    12841 aggctgcccg cctgacgatt ggcattcgtc cgctggggct gcggcagtgg tttgtggctt
    12901 ctgcccgggc cctgactgaa atgggctggt ccattgaccc gcgccggcgc tttttgcagc
    12961 agggttacct gctttggaag cgcagcatcc ccctggactg accccaccag atacaagctt
    13021 ccagttttct tttggagtgc gggcttcagc ccgcatgcct gtgcaggttc atgccttgaa
    13081 acagagctta taaaaccatt gatcatttcc tccagcccgg tttgccatgc cttcggcctc
    13141 atgctataat ccttgcccag aaagggtact accatgctgc aaaaaattgt gcgcatcctc
    13201 gggggcgacc cccaggggcg ggaactgaaa aaatatatcg aaattgttgg tcagatcaac
    13261 gcgctggaaa aaccgtatga ggctttaagt gatgaagccc tgaccgctaa aaccgctgaa
    13321 ttccgcagcc gcgtggccgc gggggagacc ctggatgacc tgctgcccga ggcctttgcc
    13381 gccgtgcgcg aggccagcaa gcgcaccatc ggtctgcgcc attacgatgt ccagttgatc
    13441 ggtggcatgg ctctgcacaa cggtcgcatt tctgaaatgc gcaccggcga aggtaaaacc
    13501 cttgtcgcca ccctgccagt ctatctcaac gcgctgaccg ggcgcggcgt tcacctcgtc
    13561 acggtgaacg attatctggc tcgccgtgat gcccgttgga tggcgcccat ctataacctg
    13621 ctgggcctga cgattggcgt tttgcaaatg gccagccgca ccgagaacgg tcgcaaggcc
    13681 ttcgtcgttg acctgaccaa aacctctccc ttcgaagatc aagacaaaat ccgcctggtg
    13741 gaccgcggcg aagcctacaa ggcagatatc gtctacggca cgaacagcga atttggcttt
    13801 gatttcctgc gtgacaacct gaccatgcgc ctgtctgagc gcgtgcagcg cgatcactac
    13861 tttgccatcg tggacgaagt ggataatatc ctcatagatg aagcccgaac ccccttgatt
    13921 atttccggcc ccgcctcgga tgacacagaa tggtatgtca agatggctca ggtggtgcgg
    13981 tccctgcgcc ccgaagatat cgaggtcaac gaacgtgacc gcaacgccat tttgactgaa
    14041 gtcggcgaag cgcatgtcga agaaatcctg ggcatccccc tgcgtgaccc cgaccgccct
    14101 gaagatatta accccgaaca ggcgcgcctg tggggttatc tggagcagtc tctcaaagcc
    14161 cagtacctct tccgccgcaa caaagattac attgttcaat ccggtaaggt cgtcattgtt
    14221 gatgagttca ccggtcggct catgccgggc cgccgctggt ccgagggctt gcatcaggcg
    14281 gtagaggcta aagaaggcgt ccgcgtggaa cctgagaacg tgacctacgc caccattacc
    14341 atccagaact acttccgcat gtatgaaaag ctctgcggta tgaccggtac agccaaaacc
    14401 gaagctgaag aatttgccaa aatttacaaa ctggatgtct tccaaattcc caccaacctg
    14461 gaatatcagg ccatgggcgg ccctgcagct cgcttcatcg aggtgaccgc taaagacgaa
    14521 cagggttaca aacaccagta ttatgcccgc cgggatgatc ccaagcaggc gcccatcttc
    14581 ttccgccgga aagactatcc agatgtgatt tatcgctctg aagaagccaa gctgcgcgct
    14641 atcactaccg aggcgctgcg ctatcacatt caaggccgcc cgcagttaat tggtaccacc
    14701 tcggttgaac attctgagcg tctttcagat cgcttctctg ccgaggccat ccgccgcctg
    14761 gcgcaggtga tgttattgcg cgatttctgg ctcaccaaaa ataatcgcca gcccaatgag
    14821 cagtccatcc cggaactggc attcttgaac aaaccgctga tggaactcaa ccccggcgag
    14881 atgcgccagt cggcccgcag cctgggcatg tcttcgctca atcccgaaga tgccgacaat
    14941 gcaacgcgct tgctgcagct ccttgaactg acccccaatg accagccccg cctgctcaaa
    15001 gtcttgcagg ctggtatcac ccaccaggtc ttgaatgccc gcaagcatga cgaagaatca
    15061 aaaatcattg cccgtgccgg cgggtttggc gctgtgacca ttgccaccaa catggccggc
    15121 cgtggtgtgg atattcgcct gggcggtgag cttgccgatg gcattcgccc ggacgtgctc
    15181 cgggtgctgc gccgggccga gtctgatctc tacaacctga ccgatgaaga actttatcgc
    15241 tccctgcagg aggttccccc ggatcaatac ggcatttacg ccgaatcggt gcaaaccttc
    15301 cagcagttta tggaagaaat ggcccgcgtg cgtgccctgg gcggcctgca tgtcattggc
    15361 tcagagcgcc atgaagcccg ccgcattgat aatcagctcc gcggtcgcgc cgcccgtcag
    15421 ggtgacccgg gttcctcccg cttctacctc tcgctggaag acgacctcat gcgcctgttt
    15481 ggcggccagc aggttgaaaa cctggtcagc cgcttcaacc tggatgaaaa cataccgatt
    15541 gaagtcggtc tggttggccg catggtcgag cagtcgcagg agcgggttga aggcgccaac
    15601 tttgacatgc gcaagcatct gctggaatat gacgatgttc tcaacagcca gcgccaccgc
    15661 atctatgccc agcgagaccg cgtctttacg aaagctgacc tctctgacga cgtggctgaa
    15721 atgctgcagg ctgaggtcaa taaacgggtg gaagctggcc tgagcgaccc cgaaggcccc
    15781 tggaagctcc tggcctacct ggaagaaatt cagccgccca tccagcacga aggcgtttct
    15841 tacccaacct acacccacca gctcctggtg gaagaaatcc agcgccgcct gcgggcctcc
    15901 ggtgctgacc tgttgaatgt gctggtgaac ctcgcggccg aagcgctgga tgccgagcgt
    15961 gaccacctcc tcaacagtgt tcgccagctc ctggaaaaac tggaagaaac ctacaaacaa
    16021 cagctctccg agcgcaccga cctgctggat acgtttatgg aagggctgaa agatcgtgaa
    16081 gatgatcagc ccgtgcgggc tcaaactgtg ctggatgagc tttctgctgc actgcgcctg
    16141 ccaattaagc tgacgcccga ccaactacgc gccctgccag aaggggggcg cccggtggtc
    16201 acagccattc gccagcaaat tgccgataac ctgattattc tcacgattac ccgcgccgtg
    16261 atcacactgg aacgccgcgt tggcgacctc ggcctgcgcc cggctcagtt ccagggcctg
    16321 gaatgggacg aagtggccga tgccctcttt accgccgtag attccgccct cattgcccgc
    16381 cgggaccgcc tggtgggtgc cgaggggcag attgcccgtg acttagcccc ccagctcgag
    16441 cggctgggag attcggcctc cttgccgaat tatctgctgg gcctgttgat cttcacggct
    16501 cagggtgccc agcaggactt tgacgcccgc acccaccgcc gcatcgtacg ccgcgtggtg
    16561 cgcctgacct atatcttcct tgcggcccat ctgctcggaa aacgctccac cgaggacgtg
    16621 tctgagtcgg tcttcaatca tctcaaaggg gctgaagctg ccatgcagat tgtctggggg
    16681 cgtgcagagt ggaatcgcct gcgccaatct gaagccaccc tggggcagtt ggatccagca
    16741 ctgcaagatc tgctgtctgc tgccctcggc actgaccgcc ttgaaaaact tgccgccact
    16801 cccctggcag aatttgagcc tgccgatcgt gatgctgcgg tcaatgtcct cggcgcccgc
    16861 ctgcaaaacg aaagctaccg cgagctgctg ctgcgcgtca tctccaacaa ctgggtagat
    16921 tacctcaccc gcattgaagc cctgcgcgtc tccatcacga tggaatccta cgcccagcgt
    16981 gaccccctgg tgcagtacaa aggccgcgcc tccgaactgt tcagcgaact gctggccgaa
    17041 attcgcagcg gtgtcgcaac ctccatgttc acctaccgcc ccacccgcgg cgccgccgcc
    17101 gcagtggacc gcactggcgc ccccgaaccg gccgccctgc cggcctctgc cccggctgag
    17161 cagcagccag tttccgccga acgcgccaag aagcgtaagc gccattagcc gctgattgat
    17221 acttttggag catgtttggg tgtaatttgc cagtcaatat tcgcgaattg cactcttgac
    17281 tcctgcagat tcgagtataa ttatctcttg tcagggttgt ctttataagc gttcccgctt
    17341 ataatcacct caatcttttg tcattggaga actgtattca tatggaccag aacaatgccc
    17401 aactcgagca gggggaaacg cccgagacta atccggtgga aaaccacacc aacatggctt
    17461 ctttgctcga agaagaaggc ctcggaattg atttcccgca ggcaggcgaa atccgcaagg
    17521 gtgtcgttgc ctcaatcacg ccaggtcaaa tcctggtcag cgtcggcacc aaatccgaag
    17581 gcgttatcag cggcaaagaa tttgagctga ttcccccgga agaactggcc gccctggccg
    17641 ttgggcagga aatccccgtt tacgtcatca accccgaaga ccaaaacgga aacgttgtcc
    17701 tgtcctatac ccgtgcccgt gaagaagtca gctgggaaca ggtggatgaa ctcctgcgcg
    17761 gtaaagaatg cattcacagc accattgttg gctacaacaa gggtggcctg attgtgccgg
    17821 ttgtgggcct gcgcggcttt gtccccgcct cccagatcag cctctcccgc cgctcccgcc
    17881 tcagcggtga cagccccgag caaatgtggg gcaagatgat caatgaagag atcgacgttt
    17941 gtgtcatcga agtggaccgc gaacgccgcc gactcattct ctctgagcgc gctgccagca
    18001 ccgagacccg cgaaaccctc aaggaacgcg tcatcgaaga gctgcaggaa ggcgaagtcc
    18061 gcaatggtcg tgtgaccagc ctggccgatt ttggcgcttt tgtcaacatc agcggcgccg
    18121 acggactcgt gcacctctcc gaaatctcct gggatcgcat tcagcacccc agcgaagtcc
    18181 tgcgcgtcgg tcaggaagtc aaagtcaaaa tcatcagtgt tgatcgcgaa aagaagcgca
    18241 ttggcctctc cattcgccag cttcaatccg atccctggtc tcagcgggtt tctcacctgc
    18301 aaattggcca actggttgaa ggcaccatca cccgccttac caagttcggc gcctttgccc
    18361 gtattggcga tgatctcgaa ggtctcattc acatctccga gatcagcgac aagcggatcg
    18421 agcaccccaa ggaagtcctt aaagaaggtg atgtggttac cctgcgcgtc attaaaattg
    18481 acgttgagaa ccaccgcatt ggcctcagcc tgcgtaaagt tgagtcgatg gctttcgccg
    18541 atatggactg gcagtcgatt gaaggcatca tcgctgatgc cggtgagatt gccgcccctg
    18601 ctgaagacat cattgctgac gctggtgagc ccgaagcccc tgctgacgag cagcccgaca
    18661 cctcggaaca ggcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtga
    18781 gcccgaagcc cctgctgacg agcagcccga cacctcggaa caggcctgaa ctgtctgaag
    18841 tagagagcgc accgaggggt gcgctctctt ttcacaccca aaatgcccat cctaatcgat
    18901 tcccacgaag acctggccta caatattctg acctttggac gggattaccg ccgttcagcg
    18961 gccgaaacgc gccgccttga agtaggtcag cgcgtattgg aagccactgg taatgccctg
    19021 ctgggctggc ctgaatacca gcgcggtcag gtggcgctgg tttgcggcac catttttgtt
    19081 ggcccgcgcc gctacctcaa gcacgattgg gaaacccaga cctatgacga ttacgccggt
    19141 gcccggcgcc tgatgcgcgc ccaggtggat gtctaccgcc gcctggccga agaatccccg
    19201 gatatgttca ccctggtgcg cacccgctcc gaactggatg cagttttggc gccctgggat
    19261 gccgcccccg ccaacccgcc gcaggtcact catcccaccg gtctggtgat cctgatggaa
    19321 agcgctgaag gccttgccga gcccgaagaa gttgaagaat ggtgggaact tggcgtccgc
    19381 atcattggcc ccgtctgggg cggctcgcgc tggtgcggcg gcacggtgga gcccggtccc
    19441 ttccgcccgg agggcttccg cctgctggat gtcatggcag acctgggttt caccctcgat
    19501 atcagccaca tgtccgatga gtcagccgtg caggcgctcg aatattaccg tggacgggtg
    19561 atcgccagcc atgccaacat ccgtgccctc atccccggcg actacagccc gcgccaactg
    19621 accgaagccg ccttgcgccg cctgatcgag cgcgatggcg tgatgggttt catccccttc
    19681 aatcgcttcc tcaaggccga ttttgtcaac agcgacccgc gtgaaaccgt cacgttggat
    19741 cattacctca agcatattga caccctctgt cagatggcgg gcaatgccca ccacgccgcc
    19801 attggcacgg attttgacgg cggctttggc tggccctggg tgcccctgga actggacacg
    19861 attgccgacc tgcagcagat tgccccgcgc ttgcagtcct ggggctattc agatgccgat
    19921 gtggctgcca ttttgggcgg caactggcgc cgcctgctcg aggagaccct tccccgatga
    19981 ccgctgccgt aaacgctgat aaccgtcagg ataccatccc agagctgccc tattcgcgcc
    20041 gccgcatccg ctgggggctg gggctcaccc tggttggctt tgtggtcttc ctgctggggg
    20101 ttcgcccggc gctctttggc gctgaccgca gcccggtggt tggctttgtg caaattgccg
    20161 tcttcctgat tggcctggct ttcatgtgca ttggtggcta catcagcatg atggcgctct
    20221 ggaagaaagc cgaacccagc attgctgccg atattggcct gcgcctggtg gcgacagggt
    20281 atgtgatcgc cgttttcgcc ggtatggcgg atgtttttgg cctgggcagt caccccctgc
    20341 ccgaagtgcc ttattttggc ctctggcagg ctcgaggcgt tgaactcggt caggccctca
    20401 ttgccattgg ctttttgctg cttttgccgc ttcgccgcct gtttcaccgc cccgagcatc
    20461 attaaacaac gcccgcgcct cgcttgaccg tctttcggcg ttgtgttatg atgattttag
    20521 aggaaaatgc gaatgaactc acaatcgaca tcggacgaaa cactccctgc cgatttacaa
    20581 actgaaaatt tggactctcc gcagcccaaa aatagttggc gtcaggcttg ggagaaaatt
    20641 gtccgcctgg gcctgggtga aattgccctg cgcaccggca ctgctgtggt ctctctggcg
    20701 ttgattttgg tggtcatctg ggtcatggca acctttttct tgaaaggcga tgcctctaat
    20761 aacctggcag gtgcggctgt acttgcggcc ccgggcacgg ttaatgccca ggcgcccacc
    20821 cccaccgccc agcccccggc ttttgtcatc ccgcaatcta ccgctcatgt caacggcatt
    20881 acccggctgg cgcagcttca taccctgctg ccttcgcgcc cccgctacga tgtcatcacc
    20941 tacaccgttc tggaaggtga taccattttt ggcattgctg aaaagttcgg cctcaagccg
    21001 cagtctattt tgctgggtaa tcgtgatctg ttgggagaca acccccacta cattcagcct
    21061 ggcatggttt tgaatattct gccctcagat ggcatctact accgctggaa cgcgggtgat
    21121 ggcctgaatg gcgtctccag ctactacaaa gttacccccg aggtgattat taactggccg
    21181 ggcaaccacc tggacccggc tactctgggt gactggtcaa accccaacat tgccgatggc
    21241 accatgctgt ttgtgcccgg cggccaggcc gagtttatca cctggagcgc cccggtgatt
    21301 acccgtgata accctggcgt agcccgcctg tttggcccgg ggtactgcgg cgtgatcacc
    21361 accggcgcag tgggtgatgg tgtctttgta tcgccgaccg tgctgaatta cctttctggt
    21421 tatgattaca cgcccgatac caaccatttc ggcctcgact ttgccggtca gatgggcacc
    21481 cccatctacg ccattgacga tggcgtggtg gtctactctg gctggaatga ccatggctat
    21541 ggatatgtca tggtgattga tcacggtaac ggctggcagt cgctgtatgc ccacctcagc
    21601 gaaatctact ttggctgcgg tcagagtgtc ttctccggcg atacgattgc cgccatgggc
    21661 agcaccggcc gctcaacggg cccgcatctc cactttgaac tgcgctataa cgggaacttt
    21721 gtaaacccct ggaatgttct gccgtaaaaa agacttatgg ctgctcacag cgacttgcgc
    21781 ccttctccca tagccggaac ctggtacagc ggtgatccgc agcggctcgg gcagcaagtt
    21841 gatgcttatc tagatcaggc cgttttaccg cccctctctg gtcgtgtggt tgggttggtg
    21901 gccccccatg ctggtcaccg ctattcgggc cgcaccgccg ggcatgcttt tgccgcagtt
    21961 cgcggtcagt cctttgatca ggtggtcgtc ttatcgcccc tgcacgactt tcaccccgcc
    22021 ccgctgctga cgacagcgca tcgtgcttat agcaccccgc tgggtccggt gccggttgat
    22081 gtccccgcgg ttgaggcgtt agacgctgct cttgctgtgc aaaacctgcg cctcacaccg
    22141 gttgccaatg accgggaaca ttccctggaa attgaactgc cgtttctcca gcgggccctg
    22201 tcgggcgatt ttcgcctcat ccccatcatg gtgcgctccc gggccacatc ggtttgccgg
    22261 gcgcttggcc tggcgctggc cgaactgctg cgtgaccatt cggccctgct ggttgccagc
    22321 accgaccttt cgcactttta cccgcttcac actgccgacg ctctggacgc cgaaatgctg
    22381 cgccgcatcg cggccttctc gcctgaagat gtgctttccg ccgaagaaac cggcgctggc
    22441 tttgcctgtg gtgccgggcc ggttgccgtg atgctctggg ctgcccgggc gctgggcgca
    22501 aatacagtga ccattctgca ccattcaacc tccgccgaag aaaccggcga tacccactct
    22561 gttgtcggct atggcgccgc tgttgccttg gggcaggggt aacgcttggc gcattttgtc
    22621 cagatagccg tcaatgttcc gctggcttcc ggggtttttg attatcacct gcccccggaa
    22681 ctggtggatt caattcaacc cggctcactg gtggtggtgc cctttggaca gcaaaccgtc
    22741 cagggccttg tcttgcgctc ggttgccgaa ccacaggttc agaaaactcg ccccgttgaa
    22801 tccctcctcg atccacaacc cgtgctgaca ccaccgcaga tggcgctggc tgcctggctg
    22861 tctgaagaga ccctggcccc gctggcgctt tgcctgcacc tcatgctccc tcccggcctc
    22921 agtcagcagg ccgataccct ttacagcctg cat
//
LOCUS       DAOPVS010000020        22421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2313_length_22421_cov_63.106590, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000020 DAOPVS010000000
VERSION     DAOPVS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 22421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22421
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2313_length_22421_cov_63.106590"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..2503)
                     /locus_tag="PKL11_03515"
     CDS             complement(113..2503)
                     /locus_tag="PKL11_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNZ12701.1"
                     /translation="MDEKTLTILEYPKILQRLADYAAFSASAELARELRPTTHLDEAL
                     LRQARTTEARRLLDVNAEVGIGGATDIRLLSERAARMAVLLPTELLEVRNTLISAREL
                     GRTFERLSAQSPRLAEIAAPLLPPPGLIEAISRAISERGEILDNASPALSSIRSELKV
                     AHERLLSRLERMVNDPKNQPILQEQIITQRNGRYVIPLRADFKGRLRSIVHDQSSSGQ
                     TLFVEPLVVVELNNRWHELQLSERDEERRILAALSAQVGEHAAELHAIVEALAELDLA
                     LMCAKYAEDLHAVEPVLVPYRNVRGGEHPGSTITLRHARHPLLDSKTVVPIDVELDER
                     TFGVVITGPNTGGKTVTLKTVGLLALMAQSGLHIPAQSGSTLSIFEDIYADIGDEQSI
                     EQSLSTFSGHITNIVRILKRANRKTMVIFDELGAGTDPQEGAALARAILSNLVEKRIP
                     CLVATHYPELKAYAHNTVGVVNASLEFNLQTLRPTYHLTIGLPGRSNALAIAERLGLP
                     GEIIQAARTMIDPTDLRAEDLLDEIHHQRDLARKARGEAEQTRARLNQQQNELNARLE
                     KIEDERQKILDKARTQAEEELEALHTELDEVRRALARAHQPVEALKPLQETVETLQET
                     VEQPVARRAVSRTEPSAARPLRPGDKVRVRSLQADGIVTALSENEVEVMIGSLRIRSR
                     LTDVQRLQTAEVEPPAPQRSVSGSAMAPFHASPGLELDLRGQRAEDALDALDRYLESA
                     ALAGLPWVRIIHGKGTGRLRVVVREALRASPHVSTFENGKDKEGGDGVTVAHLQPD"
     gene            complement(2650..3963)
                     /locus_tag="PKL11_03520"
     CDS             complement(2650..3963)
                     /locus_tag="PKL11_03520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNZ12702.1"
                     /translation="MSKLCVIGTGYVGLVTGACFADLGNDVVCMDVDASRIERLKQNI
                     MPFYEPGLEQLVARNVAAGRLSFTTDYAVALKDVEFAFIAVGTPSGVDGEADLQYVRQ
                     AAESIADLVDHSVIVINKSTVPVGTGDWVADIIRKQRNGKPLEFQVVSNPEFLREGSA
                     IGDCMNPDRVVLGSLSRPAAEKVGELYSALRCPVLITDLRTAEMIKYAANAFLATRIS
                     FINEIANMCEELGADVEEVARGMGLDKRIGPAFLEAGLGWGGSCFPKDVKALAHMAVL
                     HGTHPQLLQAVMDINRNQRRKVIYKLRKELGSLNDKVVGVLGIAFKPETDDIRESPAI
                     EIIHLLQNEGAIVRAYDPQAMANAAPALKGVILCQNPYEVAEGADALVLATKWNEFKQ
                     IDMHRIRQIMRQPILMDARNIWDPQRTREMGFTYYGVGRGFNHNA"
     gene            4097..4975
                     /gene="folD"
                     /locus_tag="PKL11_03525"
     CDS             4097..4975
                     /gene="folD"
                     /locus_tag="PKL11_03525"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF008058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNZ12703.1"
                     /translation="MTAQLLDGTALAEQVRLGVKEEVAAWVASGRPAPCLATVLVGEN
                     PASQSYVKGKRKASAEVGIDSLGYELPATATQAEVEDLVRQLNADPRVNGILVQLPLP
                     SGLDEERVLSAISIEKDVDGFHPLNIGRLAQKGRDPLFIPCTPAGVMYMLNQVMPSLE
                     GVNAVVLGRSNIVGMPVALLLVRANATVTICHSRTKNLPDVVRSADVLVAAVGRAEMV
                     RGDWVKPGAVVIDVGINRVEDATRPRGYRLVGDVAFDEAKEVAGIITPVPGGVGPMTI
                     AMLLSNTLAAAKLAHQ"
     gene            5087..7126
                     /locus_tag="PKL11_03530"
     CDS             5087..7126
                     /locus_tag="PKL11_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12704.1"
                     /translation="MKKFLSNLRFPPWTLPLALLAVCALAFGLLLPKLGFYWDDWSNI
                     HVAKIYGIPGLWEFYGDTYRPLTAWTFAVFTPLVGLSPLGWQILALVLRGLTAWAFAG
                     LLGALWPQARWQAAAAALLFLVYPVFLQQPIAVTYHRHWTGYLLYMTSLLLMVLSVQQ
                     PRRAVVFTLLALLALAGHLAIFEYYAGAELVRPLVLWLALAGAGLTGRKRLGSALLRW
                     LPYLALFGVYVIWRLLAPTVGAPDPNKPQLLLELFSQPVQAILHFTQLIIQDVVFIME
                     TSWHSTLQPALFDFSASFTLITLGVSLVAALAVAFYLTRIQTGAEPPRSWWKPALVLG
                     LAALICGGLPIWSTGRQALSGGFADRYVLASLWGASLVWVALMQAVTDIRPARAIILG
                     ILIGLSVGLHLRTANEYRWSWVHQQRYAWQLYWRAPWLQPNTTLLSDDDIFPQVRRSY
                     AVNLLYLEPRDGGKQLSYWFVRLDSDHVTDPALEFADEAVDIEYLGFTYHGTLKDSLV
                     AYYQPDQQGNCLWVLGPGDADTPGLPALTRYALSYSHPERIAPTPAASDYPLTDIFGA
                     EPTGNWCYYYEKADLARQFGDWPAVVSLGDAAQAAGFTPVDGASNSVHEWLPFIEGYA
                     RQGQWQTALDLTQSNLALDGRYTPQLCRLWQGLPAADSAGQSARAAALAALNCIP"
     gene            7144..8526
                     /locus_tag="PKL11_03535"
     CDS             7144..8526
                     /locus_tag="PKL11_03535"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNZ12705.1"
                     /translation="MSTLLVKNAALLVTMDSGRREIVNGGLFARNGFIEQVGPSDKLP
                     QTADEVIDLRDHLVLPGLVNTHHHFYQTLTRAVPAAQNANLFNWLKTLYPIWANLTPE
                     DIFISTQTAMAELALSGCTTSSDHLYLFPNGSKLDDEIEAGREIGIRLHASRGSMSLG
                     ESKGGLPPDTVVDSEDAILADSERLIRRYHDDQPGAFIRIVLAPCSPFSVTGELMKQS
                     AEMARRYGVHLHTHLAETQDEEAFCLQMFGHRPVAYMQSVNWIGPDVWFAHSVYVSKE
                     EIGLYARTGCGVAHCPTSNMRLASGIAPIREMMAAGVKVGLGVDGSASNDGSHLLAEA
                     RQAMLLARLRSGIEGASLSGEDAPPIMTARQALELATRGGAAVLGRNDIGSLEVGKCA
                     DFVAINLNRLDYAGALHDPLAAVIFCQPRGVDWNVVHGRVIVKHGVLQTVDVPHLVER
                     HNRAARRLVD"
     gene            8551..9411
                     /locus_tag="PKL11_03540"
     CDS             8551..9411
                     /locus_tag="PKL11_03540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNZ12706.1"
                     /translation="MTEAKIILGDCRDVLRDMPDNSIDLVFTSPPYADSRSHTYGGVP
                     PHEYVAWFMPIASELLRVLKPDGTFVLNIKEKVVDGERHTYVIELILEMRRQGWLWTE
                     EFIWHKKNCYPGKWPNRFRDSWERLLQFNKTRKFKMYQESVMVPMGDWAARRLKNLSD
                     TDQRRDNSRVGSGFGKNVSNWVGRDMVYPTNVLHLATETRNRNHSAVFPEGLPEWFIK
                     LFTQENDWVLDPFAGSGTTVKVAQRMNRNSIGIEILSEYYELAKSQPELMQHALFEER
                     GGYDESTSHE"
     gene            9389..10123
                     /locus_tag="PKL11_03545"
     CDS             9389..10123
                     /locus_tag="PKL11_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HNZ12707.1"
                     /translation="MNQLHMNDVVAYVEANIGSFHERRLQSLSKLKLDQVLKRKNPYL
                     FRAKNILLASDLVQQLLHAHLSSQEEAVFGEFLEGLAIFVCEQVYGGRKSNAEGIDLE
                     FDRDGIRYIVSIKSGPNWGNSGQIKNMIDDFKAAGKRLRTSNSGLVVRAVNGCCYGID
                     KSPDKGDYLKLCGQDFWTFISGNDRLYVDIIEPLGHRAKEQNENFNRAYSQIINLFVV
                     EFSARFCDDGLINWEKLVAFNSGKPQ"
     gene            10137..11930
                     /gene="ade"
                     /locus_tag="PKL11_03550"
     CDS             10137..11930
                     /gene="ade"
                     /locus_tag="PKL11_03550"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNZ12708.1"
                     /translation="MTYSPLALQDARPLVDCAMGRTPADLVIRSARWVCVQTGEIVPA
                     TDVAVIAGRIAYVGPDASHTIGNHTQIIDAEGRYLTPGLLDAHMHVESGMLTVTEFVR
                     AVAPHGTTGMFVDPHEIANVFGLKGVRLMADEALLQPIHVFVQMPSCVPSAPGLETTG
                     AAIGPAEVAEALTWPGVIGLGEMMNFPAVFNSDESIHAELTATRLSGKVIGGHYPDLD
                     LGLPFHGYVAGGPADDHEGTRVEDAVARARQGLRVMMRYGSAWHDVATQVRAVTEQGL
                     DPRGFLLCTDDSHSATLLAEGHMDRVIRHAIAQGLPPMTALQMATVNTAEHFGVSRDL
                     GMIAPGRYADMVLTSDLVNFRAELVIARGQIVAENGELLADLPCVQHPDWAVHSVHLP
                     RPLTAADFRLPSRANPAVMANVIGVIENQAPTQHLRMQVPVTHGEVHADPAHDLAKIA
                     LVERHHGTGRVQVALVQGFGFTERCAVATTVAHDSHHLIVVGTDDAEMAAAVNALAEC
                     GGGQVVVRLGQIIGKVELPIAGLMSSARAAVVARQAETVLAGFRACGCTLNNPNMQLS
                     LLGLVVIPALRISDLGLVDVSRFAHIPVLEA"
     gene            11965..12687
                     /locus_tag="PKL11_03555"
     CDS             11965..12687
                     /locus_tag="PKL11_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ12709.1"
                     /translation="MTENQLFQRLQAQLSALIEQTPAGQRLPSEPDLAKELGVSRATL
                     REAMRAFEAQGQIRRRQGVGTFVVGAPGVMETGLELLESIETLAKRIQLPVSLGAIDI
                     NPLLADAAQAEQLRVAEGTPLVEVSRTILTADRPVAFLIDLLPQEYLLPEDLQQGFSG
                     SVLDVLLHRGAPPLARSVAQIQAVSAKPQIAKKLQIQRGDVLLLLEAQLYGADGRIVD
                     YSHSYFIPGHFRFHVVRRVGTA"
     gene            12711..13922
                     /locus_tag="PKL11_03560"
     CDS             12711..13922
                     /locus_tag="PKL11_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNZ12710.1"
                     /translation="MDKQVIDAVQSRVRQHRDDMIRFMREIVAIPSMDSKIGPVGERI
                     AAEMRLLGYDEVRFDVMGNILGRIGSGPRVIVFDSHIDTVGIGDPAEWEFDPFQGKVE
                     DGILYGRGACDEKNSTPGMVYGLAIARDLGLLDGWTVYYFGNMEEWCDGIAPNSFVEV
                     DPKVRPDFVVIGEPTKMNVYRGHKGRIELRVVAKGKSAHAASNHLGENAIYMLLPVIA
                     GIRDLEASLGDDPFLGHGKITVSDMTVKTPSINAVPDEAVIYIDRRMTFGETKEAAIA
                     QVEALIPAELRDKVKVEALFYDEPSYTGFVFPVDKYFPAWALDEAHPLVQAGLEARRA
                     MGLTDAPAGKWAFSTNGIYWAGKAGIPSIGFGPADEETAHTTRDSVPLDDLVRAAEFY
                     ALLPAILKARL"
     gene            13971..14948
                     /locus_tag="PKL11_03565"
     CDS             13971..14948
                     /locus_tag="PKL11_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNZ12711.1"
                     /translation="MQTYLHGRDLIGDLDFSKEEVETVLDVAFDLKRKRALGEPHAYL
                     RDKVLAMLFFFSSTRTRGSFEAGIAQLGGHGAFIESSTTQIKHGDTAKEIGEIYGRYF
                     DAIAIRHCDWQIGNAYLNEVARNCRVPVLNMQCDVYHPFQCLADLMTIVEKKGRDLRR
                     KKIVVSWAYAASYLKPISVPQSLILQMPRFGMDVVLAYPKEFMLMPEIEAEAQAQARK
                     FGTGFEIVHDMDEGCRDADVIYAKSWGPLLTTSDAAQGKVLQDSHIDWIMDQRRLRLA
                     KEDAIYMHPLPADRNIEVTDEVIDGPNSVVYDEAENRLHVQKAVMALTM"
     gene            14991..15938
                     /gene="arcC"
                     /locus_tag="PKL11_03570"
     CDS             14991..15938
                     /gene="arcC"
                     /locus_tag="PKL11_03570"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNZ12712.1"
                     /translation="MTKKRVAVVAVGGNSLIKDEKRKSIPDQYEASVESMRHIVDMVE
                     QGWEVVVTHGNGPQVGFIHRRSEISRHELHEVPLDYCGADTQGSIGYMFVQALHNEFR
                     LRGIKKHAVAVVTQTLVDPHDQAFQHPTKPIGSFMDEATAHERAAREGWVVVEDAGRG
                     WRRVVASPIPQEIVEADAIHKLINAGFMVVGVGGGGIPVVKLDNGDLKGVEAVIDKDF
                     GSSLLATMIGANLFIISTAVEKVAINFNKPNQQWLDRMTVAEAKQYIAEGHFAKGSML
                     PKIQAILRFLEHDGGEALITNPENIGRALRGETGTWIVP"
     gene            16108..17334
                     /locus_tag="PKL11_03575"
     CDS             16108..17334
                     /locus_tag="PKL11_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ12713.1"
                     /translation="MSSKRLWFVVSLMLVLAMALAACKTPTTELTQQPEVETEELVFG
                     MLLVGPYNDHGYSQAHYDAGLYVEENFDGARMVYVDKVNPADRPGTTADQLAEELLAK
                     GATVIIFNSDDMKDAAVTFAQAHPEVIVIHASGDNAWKDGQNYKALPNLSNVMGRMEF
                     GKMMAGCAAALTTETGKIGYLGPLINDETRRLASSAYLGARYCYEKYRGENPDDLEFK
                     VTWIGFWFNIPGVTSDPTQVANDFYDKGYDVVISGIDTTEALVQAKQKTAEGKKVFAI
                     PYDFMGACAEAENVCLGVPYFNWGPAYLSMLKAADAGTLQPTWDFVGPDWKDINNLDT
                     SAIGFIKGPALPADAGTNLDKFIAELAGGLNLWTGPIKLQDGSEYLKAGEVATDFQVW
                     YLPQLLDGMEGQSVSE"
     gene            17403..18866
                     /locus_tag="PKL11_03580"
     CDS             17403..18866
                     /locus_tag="PKL11_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ12714.1"
                     /translation="MNVELRNIHKHFGKVHANNDITLNIPAGVIQGILGENGAGKSTL
                     MKVLSGYIHADSGEILLDGQPVRITSPADAIRCGVGMLHQDPLDFPPMRIIDNFILGQ
                     PGGLLPNRRQAEREFHELRTRFDFNINPDAYVDSLTVGERQQLEILRLLWLGARVLIL
                     DEPTTGISAAQKTKLFATLRILAEQGKTAIFVSHKLEDVEELCHRVAVLQRGRLVGEA
                     APPYVTDDLVRMMFGKGIKLADRAALSSGKCGLSVTDLSIEDVRLNLRHISLEVNCGE
                     VIGLAGMEGSGQRQFLRVCGGLQRPVGGHLHLNGHDLTGKPYRNFLDAGVAYVPASRL
                     EEGLIPGLTLTEHFILTGKQPGFFINRAAAQKRTEQGIEDFSIRGTPASQVERLSGGN
                     QQRALLALQRSPLNLLLLEHPTRGLDIESSIWIWGKLKERCRQGTSIIFISADLDEIL
                     HYSDRVLVFFGGRVSEPLDAASTSVDQLGQLIGGKGF"
     gene            18871..19908
                     /locus_tag="PKL11_03585"
     CDS             18871..19908
                     /locus_tag="PKL11_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12715.1"
                     /translation="MKSFRWSTVGFQVLAVVLALAFTSLVLMIAGTPPLAAYANILNG
                     AFNSMKSFADVAVAWIPLLIVTAGLLVTFSAGLWNIGIEGQIMVGAIFTTGALRLLQD
                     SALPSALIIVAGFLAGMLGGALWAALAGLLKIYGGVNEIFGGLGLNFVATALALWLIF
                     GPWKRPGVGSMSGTEPFPEALWLPLLPGTRLSGWSVAVGLVGIIVIYFLLQGTYFGLR
                     LKAVGRNARAAFLLGIPTSMYGLLAFLICGALAGAAGAVQVTGVYHRLIPSISSGYGF
                     LGLMVAMLVNYQAIWAVLVSLFFAALNIGSIQLPIVLKVDSSLAGVLQGALVLFFMLM
                     DGVRRRFQPKS"
     gene            19919..20914
                     /locus_tag="PKL11_03590"
     CDS             19919..20914
                     /locus_tag="PKL11_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12716.1"
                     /translation="MDSAFLSGLAGILAAAAPILFAAIGETLTERAGVTNLSVNGAII
                     LTAMVGFVVAVETNSTVLGFLAGAVVGALVTLLLAFATITLKQSQVAVGFVLALLCRD
                     LAYFLGNPYMGVSGPIVSHLPIPLLADIPIIGQIFFRHNLPVYASYLLVFLAFWWLFK
                     TRPGLMLQGIGERPEAAFARGANVNRMRYFYTVLGGALVGIAGPLYSLSVKAGWKGTI
                     SGLDGIGWIALAITIFGAWNPMRAALGAYFFAFLQWLGLVLQPSLPGIPSQVLQVAPF
                     PLMILTLLLVNIGNAEWVDRALAAMPEKTRHFISRLLRAMRSSPPGALGVPFDNE"
     gene            20921..22168
                     /gene="thrC"
                     /locus_tag="PKL11_03595"
     CDS             20921..22168
                     /gene="thrC"
                     /locus_tag="PKL11_03595"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ12717.1"
                     /translation="MAAFLGYRCSLCQAEYTPGQVTYTCPRDGGNLDVVLDYAALRQK
                     YEPADLTSRTEESLWRYLPLLPVADPEAEGTPLNRAGWTPLYTPSRLAESLDLRQLWI
                     KDEGRNPTASFKDRASAVVVARARQLQAEIVVTASTGNAGAALAGMAAAVGQPAVIFA
                     PRSAPPAKVAQLLVYGARVILVDGSYDQAFDLSVLAAQEFGWYCRNTGYNPFTLEGKK
                     TAAFEIWEQVILNLSPSSRPLTVFVSVGDGNIISGIHKGFKDLQALGWLDQMPRLIGV
                     QAEGSAAVANAFRSGVETIIPVQAQTLADSISVDLPRDGVRAVRAATQTGGAYLTVSD
                     VEILSAIAELGRVGIFAEPAAATAYAGLMSAVSQEMVRPEDPVLVMNTGSGLKDVRAA
                     MQAVQAAPIIEPTMPALRRLLAI"
BASE COUNT         4237 a         6942 c         6400 g         4842 t
ORIGIN      
        1 taagattatc aaatatgctt tccggggcgc tggcgaaggg agccacgcaa aaagaggcca
       61 atcgtgtagg ggacttgcac gattggcctt tatcatttaa ttcatcggtt ggttaatcgg
      121 gctgcaggtg cgccacagtc accccatcgc cgccttcttt gtccttcccg ttttcaaacg
      181 tagagacatg cggcgaagcc ctcagcgcct cgcggacaac cacgcgcagc cgcccggttc
      241 cctttccgtg aatgatccgc acccacggca gccctgccaa cgccgcagat tccaggtagc
      301 ggtccagcgc atccagcgca tcttcggcac gctggccgcg caggtccagt tccaatcccg
      361 gtgaggcgtg aaaaggcgcc atagcgctgc cagagacact gcgctgcggc gcaggcggct
      421 caacctcagc cgtttgcagg cgctgcacat ccgtcagccg cgagcgtatt cgcaggctgc
      481 cgatcatcac ctccacttcg ttctcgctca gcgcggtcac aatgccatcc gcttgcagcg
      541 agcgcacgcg caccttgtcg ccggggcgca ggggccgcgc cgcgctgggt tcagtccggc
      601 tgaccgcgcg gcgcgctacg ggctgttcaa cggtttcctg cagggtttca acggtttctt
      661 gcaatggctt cagggcttca accggctgat gtgcccgcgc cagggcgcgg cgcacctcat
      721 ccagctcggt atgcagggct tccagttctt cttcagcctg ggtgcgggcc ttgtccagaa
      781 tcttttgccg ctcgtcttca attttttcca accgggcatt cagttcattt tgctgttgat
      841 tcagccgggc gcgggtttgt tcggcctcgc cgcgggcttt tcgggccagg tcgcgctgat
      901 gatgaatctc atccagcaga tcttcggcgc gcaggtccgt cgggtcaatc atggtgcgcg
      961 ccgcctggat gatctcgccg ggcagcccca gccgctcggc aattgccagc gcattcgagc
     1021 gccccggcag gccaatcgtc agatgatagg tcggccgcag cgtttgcagg ttgaactcca
     1081 ggctggcatt gaccaccccc accgtgttgt gggcataggc cttcaattcg gggtaatgcg
     1141 ttgccaccag gcaggggatc cgcttttcca ccagattcga caggatggcg cgcgccagcg
     1201 cagcgccttc ctgcgggtcg gtcccggccc ccagttcatc aaagatgacc atggtcttgc
     1261 ggttggcgcg cttgaggatc cgtacgatgt tggtgatatg ccctgaaaac gtggataacg
     1321 actgttcaat ggactgctca tcgccaatgt ctgcatagat atcttcaaag atactcagcg
     1381 ttgagcccga ctgcgccgga atgtgcagcc cgctctgcgc catcaaggcc agcagcccca
     1441 cagttttgag ggtgaccgtc ttgccgccgg tgttcggtcc cgtaatcacc acaccaaacg
     1501 tgcgttcatc cagctcaaca tcaatcggca ccacggtctt tgaatccagc agcggatgcc
     1561 gggcatgtcg cagcgtgatg gtgctgccgg ggtgttcccc gccccggacg ttgcgatagg
     1621 gcaccagcac cggttcaacc gcgtgcaggt cttcagcata cttggcgcac atcaaggcca
     1681 gatccagctc agccagggct tccacaatcg cgtgcagctc tgcggcatgc tcgcccacct
     1741 gtgcagacag cgccgccagg atgcggcgtt cttcgtcgcg ctcggacagt tgcagttcat
     1801 gccagcggtt gttcaattcc accaccacca gcggctccac aaacagggtc tgcccggagg
     1861 aggattggtc gtgaacgatg gagcgcagcc gccccttgaa gtctgcgcgc agcgggatca
     1921 catagcgccc gttgcgctgg gtgatgattt gttcctgcag gatcggctga ttcttggggt
     1981 cgttgaccat ccgctccagc cggctgagca gccgctcgtg cgccactttg agttctgacc
     2041 gaatggaaga cagcgccggt gaagcattgt ccagaatctc tccgcgttcc gagatggccc
     2101 gcgagatggc ctcaatcagg ccgggtgggg gcagcagcgg tgccgcaatt tcggccagcc
     2161 gtggcgactg ggcggatagg cgttcaaagg tgcgccccag ctcgcgtgcg ctgatcaggg
     2221 tattgcggac ttccagtagt tcggtgggca gcagcaccgc catccgcgcc gcccgttcag
     2281 acagcagccg gatatcggtt gcgccgccga tgcccacttc ggcattcacg tccagcaggc
     2341 ggcgcgcttc ggtagtgcgc gcctgccgca gcagggcttc gtctagatgg gtggtggggc
     2401 gcagttcgcg cgccagttcc gccgaggcgc tgaaggccgc ataatcggcc agccgctgga
     2461 ggatcttggg atattccaat atggtaagtg ttttttcgtc catggtttct tgtccatcac
     2521 cgcgccgccc gatttttaac agcaatcgcg ggcgccgcgc tttaagcaga tagggtttga
     2581 aaattcagtt tcaaacttga attgatctgc gcagccgcag cgcccgcgaa tttttgaaaa
     2641 gcttttcagc taggcgttgt gattgaatcc gcgtcccacc ccgtaatagg taaagcccat
     2701 ttcacgggtg cgctgcgggt cccagatgtt gcgggcgtcc atcagaatgg gctgccgcat
     2761 aatctggcgg atgcggtgca tatcaatttg tttgaattcg ttccatttgg tcgccagcac
     2821 cagcgcgtct gcgccttcgg cgacttcata cgggttctgg cacagaatga cgcccttgag
     2881 tgccggggcg gcattggcca tcgcctgcgg gtcataagcg cgcacaatgg cgccttcatt
     2941 ttgcagcagg tgaataatct caatggccgg tgactcgcga atatcgtccg tctcgggttt
     3001 gaaggcgatg cccagcacgc cgaccacttt gtcgttcagg ctgcccagtt ccttgcgcag
     3061 cttgtagatc accttgcggc gctggttgcg gttgatgtcc atcacggctt gcaagagctg
     3121 cgggtgagtg ccgtgcagca cagccatatg cgccagcgct ttcacgtcct tggggaagca
     3181 gcttccgccc caacccagcc cggcttccaa aaatgccggg ccaatgcgct tatccaggcc
     3241 catgccgcgc gccacttctt ccacatcggc gcccagctct tcacacatat tcgcaatctc
     3301 attaatgaag gagatgcgcg tggccaggaa ggcgttggcc gcatatttga tcatttctgc
     3361 cgtgcgcagg tcggtgatca gcaccggaca gcgcagtgca gaatacagct cgcccacttt
     3421 ttcagccgcg gggcggctca gcgaccccag caccacccgg tccgggttca tgcagtcccc
     3481 aatggctgag ccctcacgca gaaattccgg gttggataca acctgaaatt ccagcggctt
     3541 gccattgcgc tgcttgcgga tgatatcagc cacccagtcg ccggtgccca ctggcacggt
     3601 ggatttgttg atcacaatca ctgaatgatc caccaggtcg gcaatgcttt cagccgcctg
     3661 ccgcacatac tgcaaatcag cctcgccatc cacgccagaa ggcgtgccga cggcaataaa
     3721 ggcaaactca acatccttca acgccactgc atagtcagtg gtaaagctca atcgtcccgc
     3781 ggccacattg cgcgccacca actgctccag gccgggttcg taaaacggca taatattttg
     3841 cttcaaccgt tcaatccggc ttgcatctac atccatgcaa accacgtcat tgcccaggtc
     3901 ggcaaaacag gcccccgtca ccaggccaac atatccggtt ccaattacac agagtttgct
     3961 catcggttta tttgatctcc tgaaagcgga ttataacatc agtttgcccg cgcgcacttt
     4021 tgtgatgcag gtataatcaa tggaaacctt tgaaattatt cccgtgcacc agaatttttg
     4081 aactcctgga ggacatatga ccgcacaact tttagatggt accgcacttg ccgaacaggt
     4141 tcgcctgggg gtaaaagaag aagtcgctgc ctgggtggca tcgggtcgcc cggcgccctg
     4201 cctggcaacc gtattggttg gcgaaaaccc ggcctcacaa tcctatgtca agggcaagcg
     4261 caaggcctcg gccgaagtcg gcattgattc gctgggctac gagctgcccg ccaccgccac
     4321 ccaggccgag gtggaagatc tggtccgcca gctcaacgcc gacccgcgcg tcaacggcat
     4381 tctggtgcag ctcccactgc cttcaggtct ggacgaggag cgcgtgctca gcgccatcag
     4441 catcgaaaaa gacgtggatg gtttccatcc cctcaacatt ggccgcttgg cgcaaaaagg
     4501 ccgcgacccg ctctttatcc cctgcacccc ggcgggtgtg atgtatatgc tcaatcaagt
     4561 catgccctcc cttgaaggcg tcaatgcggt ggtgctgggg cgctccaata ttgtgggcat
     4621 gcccgtggcg ctgctgctgg tgcgcgccaa tgccaccgtc accatttgcc actcccgcac
     4681 caaaaacctg cccgatgtcg tccgctcggc cgatgtgctg gtggccgcgg tgggccgcgc
     4741 tgaaatggtg cgcggcgatt gggtcaaacc cggcgcggtg gtgattgatg tgggcattaa
     4801 ccgtgtggaa gatgccaccc gcccgcgcgg ttatcgcctg gtgggcgatg tggcctttga
     4861 cgaggccaaa gaagttgccg gcatcatcac accggtgccc ggcggcgtag gtcccatgac
     4921 cattgccatg ctgctcagca ataccctggc tgccgccaaa ctggcacacc aataaagcca
     4981 gccgtttgtc taaatgtctg acaattgatt gacattttgg ggcgttttgg ttaaactcta
     5041 atcaccaaac gccccatttt tgtctgtgta tttgcaaagg gaatccgtga agaagttcct
     5101 gtccaatctt cggtttccac cctggacgct tcccctggct ctgctggctg tttgcgcgct
     5161 cgcgtttggc ctcctgctcc caaaactggg gttttactgg gatgactggt ctaacatcca
     5221 cgttgccaaa atctacggca ttcccggcct gtgggaattt tacggcgaca cctaccgccc
     5281 gctgaccgcc tggacctttg ccgtattcac cccgcttgtc gggttgagcc cgctcggctg
     5341 gcagattctg gcgctggtgc tgcgcggcct caccgcctgg gcttttgccg ggctgctggg
     5401 cgcgctctgg ccccaggccc gctggcaggc cgccgcggcg gcgctgcttt ttcttgtcta
     5461 tccggtcttt ctccagcagc ctatcgctgt cacctaccac cgtcactgga ccggctacct
     5521 gctctacatg acttccctgc tgttgatggt tctgtctgtc cagcagccgc gccgggcggt
     5581 ggtcttcacc ctgcttgcct tgctggccct ggctggccat ctggccattt ttgaatatta
     5641 cgccggcgcc gaactggtgc gcccgctggt gctctggttg gccctggccg gggctgggct
     5701 gaccgggcgc aaacgtctag ggtcggccct gttgcgctgg ctgccttacc tggcgctgtt
     5761 tggtgtttat gtgatctggc gcttgctggc accaaccgtg ggtgcacccg accctaacaa
     5821 gccccagctt ctgctggagc tgttctccca gcccgtgcag gccattttgc acttcaccca
     5881 gctcatcatt caggatgtgg ttttcattat ggagaccagt tggcacagca ccctgcagcc
     5941 cgccctgttt gatttttccg cctcattcac gctcatcacc ctgggcgtca gcctggttgc
     6001 cgcgctggcg gtcgccttct acctgacccg catccagact ggcgccgagc cgccgcgttc
     6061 ctggtggaag cccgccctgg tgctgggcct ggcggcattg atctgcggcg gcctgcccat
     6121 ctggtccacc gggcggcagg cgctcagcgg cggctttgcc gaccggtatg tgctggcctc
     6181 gctgtggggg gcatccctgg tttgggttgc gctgatgcag gccgtgaccg atatccgccc
     6241 ggcgcgggcc atcattctgg gcattctgat tggcctcagc gtggggctgc acctgcgcac
     6301 cgccaacgaa taccgctggt cgtgggtgca ccagcagcgg tatgcctggc agctctactg
     6361 gcgggccccc tggctgcagc ccaacactac cctgctctct gatgatgata tctttccgca
     6421 ggtgcgccgc tcgtatgccg tcaacctgct ttacctcgaa ccgcgcgatg gcggcaagca
     6481 gctttcctac tggtttgtcc ggctggattc ggaccacgtg accgatccag cgcttgaatt
     6541 tgccgacgaa gccgtggaca ttgaatacct gggctttacc taccacggca cgctcaaaga
     6601 cagcctggtg gcctactacc agcccgacca gcagggcaac tgcctctggg tactcggccc
     6661 tggcgatgcc gatacccccg gcctgcccgc gctcacccgc tacgcgctgt cctattcaca
     6721 tcccgagcgg attgcgccaa ccccggccgc ctcagactac ccgctgaccg atatcttcgg
     6781 cgctgaaccg accggtaact ggtgctatta ctatgaaaaa gccgacctgg cccgtcagtt
     6841 tggcgactgg cctgcagttg tctcccttgg cgatgctgct caggcggctg gcttcacccc
     6901 tgtcgatggg gcttcaaatt cagtgcatga gtggctgccc tttattgagg gttatgcccg
     6961 ccagggtcaa tggcaaaccg ccctcgacct gacccagtcc aacctcgccc tcgatgggcg
     7021 ctacaccccg cagctttgcc gcctgtggca ggggcttcct gctgccgatt ccgccggcca
     7081 atcggcccgc gccgcggccc tggctgcttt gaattgcatc ccctgattga aaggagttat
     7141 cacatgagca ccctcttggt taaaaacgcc gctttactgg tcacaatgga cagcggccgc
     7201 cgcgagatcg tcaatggcgg cctgtttgcc cgcaacggct ttattgagca ggttggcccc
     7261 agcgataaat taccccagac cgccgacgag gtgattgacc tgcgcgatca cctggtgctg
     7321 cccggcctgg tcaataccca ccatcacttt taccagaccc tcacccgcgc tgtgcctgcc
     7381 gctcagaatg ccaacctgtt caactggctg aaaaccctgt accccatctg ggccaacctg
     7441 acccctgaag atatcttcat ctctacccag accgccatgg ccgagctggc cctgtcgggc
     7501 tgcaccacct catctgatca cctgtatctc ttccccaatg gctccaaact ggatgatgag
     7561 attgaagccg gtcgcgaaat cggcattcgt ctgcatgcct cgcgtggctc gatgagcctg
     7621 ggcgagagca aaggcggcct gccgccagac actgtggtcg attcagaaga tgccatcctg
     7681 gccgactctg aacgcctgat ccgccgctat cacgatgacc agcccggcgc gttcatccgc
     7741 attgtgctgg cgccctgctc ccccttctct gtgaccggtg aactgatgaa acagagcgct
     7801 gaaatggccc gccgctacgg cgtccatttg cacacccacc tggccgaaac ccaggacgaa
     7861 gaagcctttt gccttcagat gttcggccac cgtccagtgg cctacatgca gtcggtgaac
     7921 tggattggcc cggatgtctg gtttgcccac tcggtctatg tcagcaagga agaaatcggc
     7981 ctctatgccc gcactggctg cggcgtggcg cactgcccca cctccaacat gcggctggct
     8041 tcgggcattg cacccatccg tgagatgatg gctgccggcg tcaaggtcgg cctcggtgta
     8101 gatggctccg ccagtaacga cggctcgcac ctgctggcag aggctcgcca ggccatgctg
     8161 ctggcccgcc tgcgctccgg cattgaaggc gcctcgctca gcggcgaaga cgccccgccc
     8221 atcatgaccg cccgccaggc cctcgaactg gcaacccgcg gcggcgccgc cgtgctcggc
     8281 cgcaacgaca tcggctcgct ggaagtcggt aaatgcgccg acttcgtcgc catcaacctc
     8341 aaccggcttg actatgctgg cgccctccac gacccgctgg cggcagtcat cttctgccag
     8401 ccccgcggcg tggactggaa cgtggtgcac ggccgtgtta tcgtcaagca cggtgtcttg
     8461 caaaccgtgg atgtccccca cctggttgag cgccacaacc gcgccgcccg gcgattagta
     8521 gactgatgaa attgacgtgg agactttaga atgactgagg caaagattat tttaggagat
     8581 tgtcgagatg ttctccggga catgccggat aactcgattg accttgtctt tacctcgccg
     8641 ccctatgccg acagccggtc acatacttac gggggggttc ctccgcacga gtatgtggcg
     8701 tggtttatgc cgattgcctc tgagttattg cgagtcctga agccagacgg cacgtttgtt
     8761 ctgaatatca aagaaaaagt cgttgatggc gaacgacaca cctatgtcat tgagctgatc
     8821 ttggaaatgc gccgccaggg ctggctttgg acagaagaat ttatctggca caagaaaaat
     8881 tgctatccgg gcaagtggcc gaatcgattc cgtgattcgt gggagcgcct gttacagttt
     8941 aataaaaccc gaaaatttaa gatgtatcag gaatctgtga tggtgccaat gggcgactgg
     9001 gcagctcgcc gcttgaaaaa cctcagcgat acagaccagc gccgggacaa ttctcgcgtg
     9061 ggcagcggct ttggaaaaaa cgtgtcaaat tgggttgggc gagatatggt ttacccgaca
     9121 aatgtactgc accttgccac agaaacccgt aaccgaaacc acagcgctgt ctttccagaa
     9181 ggcttaccag aatggtttat caagctcttc acccaggaaa acgattgggt gttagatccc
     9241 tttgctggtt ctggcacaac ggtaaaagtt gcccaacgga tgaacagaaa ttcgataggg
     9301 attgaaattt tatcggagta ttacgaactg gcaaaatcac aacccgaatt aatgcagcat
     9361 gcattgtttg aagaaagggg aggatacgat gaatcaactt cacatgaatg atgtggttgc
     9421 ttatgttgag gccaatattg gctcattcca cgagcgtcgg cttcaaagct tgagtaagtt
     9481 gaaattagat caggtactga agcgaaaaaa cccttaccta tttcgcgcca agaatatcct
     9541 tttagcttca gatctggtcc agcagctctt acatgctcac ctttcctcgc aagaagaagc
     9601 tgtttttgga gaatttcttg aaggattggc gatttttgtc tgcgaacaag tttatggcgg
     9661 acgaaaatca aatgcagaag gtatagacct tgaattcgac cgggatggta tccgctatat
     9721 tgtttcaatt aagtccggac ccaattgggg aaacagtggt cagattaaga acatgatcga
     9781 tgattttaaa gctgccggaa aacggttacg aacatcaaac tctggccttg tcgtccgtgc
     9841 agttaatgga tgctgttatg gcattgataa atctccagac aagggcgatt acctgaaatt
     9901 gtgcggccaa gatttttgga cttttatttc aggcaatgac cgactctatg tagatattat
     9961 tgaacctttg ggacatcgag ccaaagaaca gaatgagaat ttcaaccgag cgtatagcca
    10021 gattatcaat ctatttgtgg ttgaattttc agctcgattt tgtgatgatg gactgattaa
    10081 ctgggaaaaa ctagtggctt ttaattcggg taagccacaa taaggattat tggataatga
    10141 cctattcccc cctcgccctt caagatgccc gcccgctggt ggattgtgcc atgggccgca
    10201 cccccgccga ccttgtcatc cgctcggcgc gctgggtctg cgtgcagacc ggcgaaattg
    10261 tccccgccac cgatgtagct gtcattgccg gccggatcgc ctatgttggt cccgatgcct
    10321 cgcacaccat tggtaatcac actcagataa ttgatgctga gggccgctac ctgacccccg
    10381 gcctgctgga tgcccacatg cacgtcgaat cgggcatgct caccgtcact gagtttgtgc
    10441 gtgccgtggc tccgcatggc accaccggca tgtttgttga cccccacgaa atcgccaacg
    10501 tctttggcct caagggcgtc cgcctgatgg ccgatgaagc gctgctgcag cccatccatg
    10561 tctttgtgca gatgccctcc tgcgtgcctt ctgcccccgg tctcgagacc accggcgctg
    10621 ccattggccc ggccgaggtg gctgaggccc tcacctggcc cggcgtcatc ggcctgggcg
    10681 agatgatgaa cttcccggcg gtcttcaaca gcgatgaatc cattcacgcc gaactgaccg
    10741 ccacccggct ttccggcaag gtcattggcg gccactaccc cgatcttgat ctcggcctgc
    10801 ccttccacgg ctatgtggct ggcggtcctg cggatgatca cgaaggcacc cgggtggaag
    10861 atgccgtggc ccgcgcccgt cagggtctgc gggtgatgat gcgctacggc tcagcctggc
    10921 acgatgttgc cacccaggtg cgcgccgtca ccgagcaggg cctcgatccg cgcggcttcc
    10981 tgctctgcac cgacgactca cattcggcca ccctgctggc tgaaggccac atggatcgtg
    11041 tcatccgcca tgccattgcc cagggcctgc cgcccatgac cgccttgcaa atggctacgg
    11101 ttaataccgc tgaacatttt ggcgtcagcc gcgacctggg gatgattgcc cccgggcgtt
    11161 acgccgacat ggtgctgacc tcagacctgg tcaatttccg cgccgaactc gtgattgccc
    11221 gcggccagat tgtggccgaa aacggcgaac tgctggccga tctaccctgc gtgcagcacc
    11281 ccgattgggc ggttcactcc gtgcatctgc cccgtccgct caccgcggcc gacttccggc
    11341 tgcccagccg cgccaacccc gctgtgatgg ccaacgtcat cggcgtgatt gaaaatcagg
    11401 cccccaccca acacctgcgt atgcaggtgc cggtaactca cggcgaagtt cacgccgacc
    11461 ccgcccacga tctggcaaaa atcgccctgg tggaacgcca ccacggcacc gggcgggtgc
    11521 aggtggcgct ggtgcagggc tttggcttca ccgagcgctg cgctgtagcc accaccgtgg
    11581 cccacgacag ccaccatctc atcgtggtgg gcaccgatga tgccgaaatg gctgccgcgg
    11641 tcaacgccct ggcggaatgc ggcggcgggc aggtggtggt gcgcctgggg cagattattg
    11701 gcaaggtgga actgcccatt gccggtctga tgtcttcggc ccgcgccgcc gtggtggcgc
    11761 gccaggccga aaccgtgctg gcaggcttcc gcgcctgcgg ctgcaccctc aacaatccca
    11821 atatgcagct cagtctgctg ggcctggtcg tgatccccgc cttgcgcatc tcagacctgg
    11881 gcctggtgga tgtaagccgc tttgcccata ttccagtttt ggaagcctaa cggtccgggt
    11941 catatttccg aggagcaatg aacgatgaca gaaaatcaac tttttcaacg attgcaggcc
    12001 cagctatcag cgctgattga acaaaccccg gcggggcagc ggctgccttc tgagccagat
    12061 ctggctaaag agcttggcgt ttcgcgcgcc accctgcgcg aagccatgcg cgcttttgag
    12121 gcccagggtc aaatccgccg ccggcagggg gtgggcacct ttgtggttgg tgcccccggc
    12181 gtgatggaga ccggcttgga gctcctggaa agcattgaaa ccctcgccaa acggattcaa
    12241 ctgccggttt ctcttggcgc gatagatatt aaccccctgc tggcagatgc tgcccaggcc
    12301 gagcagctgc gtgtagccga aggaaccccg ctcgttgagg tctcgcgcac catcctgacc
    12361 gctgaccgcc ccgtggcctt tctcattgac ctgctgccgc aggaatacct gctccccgaa
    12421 gatttgcagc aaggcttcag cggctcagtg ctcgatgtgc tgctgcaccg cggcgccccg
    12481 ccgctggccc gctcggtggc gcaaattcag gccgtttcgg ccaaaccgca aattgccaaa
    12541 aaactccaga tccagcgcgg cgacgtgctg ctgctgctgg aggcgcagct ttacggcgcc
    12601 gatgggcgca ttgtggacta ttcgcacagc tatttcatcc ccggccattt ccgcttccat
    12661 gtggttcgcc gggtgggcac agcctgattt ttataggagg taaaaggata atggataagc
    12721 aagtcattga tgctgtacag tcgcgtgtcc gccagcaccg cgacgacatg atccgcttca
    12781 tgcgcgagat cgtggctatc cccagcatgg actctaagat cggtccggtt ggggaacgga
    12841 ttgccgccga aatgcggctg ttgggctatg acgaagtgcg tttcgatgtg atgggcaaca
    12901 tcctcgggcg tatcggttca ggcccgcgtg tcatcgtctt cgattcccac attgacaccg
    12961 tgggcatcgg cgaccctgcc gaatgggaat ttgacccctt ccagggcaag gtcgaagacg
    13021 gcattctcta cggccgcggc gcctgtgacg agaaaaacag cacccccggc atggtctatg
    13081 gcctggcgat tgcccgcgac ctgggcctgc tggacggctg gacggtctat tactttggca
    13141 acatggaaga atggtgcgat ggcatcgccc ccaattcctt tgtcgaagtg gaccccaagg
    13201 tgcgtcccga ttttgtcgtc attggtgaac cgaccaagat gaatgtctat cgcggccaca
    13261 agggccgtat tgagctgcgt gtggtggcca agggcaagag cgctcatgcc gccagcaacc
    13321 acctcggcga aaatgccatc tacatgctgc tgcccgtcat cgccggcatc cgtgacctgg
    13381 aagccagcct gggtgacgac cccttcctgg gacacggcaa aatcaccgtc agcgatatga
    13441 ccgtcaagac ccccagcatc aatgccgtgc ccgatgaggc tgtcatctac attgaccgcc
    13501 gcatgacctt tggcgaaacc aaagaagccg ccattgccca ggttgaagcc ctcatccccg
    13561 ccgagctgcg cgacaaggtc aaggttgagg cgctgttcta tgatgagcca tcttacaccg
    13621 ggtttgtctt ccccgtggac aagtacttcc ccgcctgggc gctggatgaa gctcacccgc
    13681 tggtgcaggc tggccttgaa gcccgccgcg ccatgggcct gaccgatgcc cccgccggaa
    13741 aatgggcctt cagcaccaac ggcatctact gggcgggcaa ggctggcatt cccagcattg
    13801 gctttggccc cgcagacgaa gagaccgccc ataccacccg cgactctgtg ccgctggatg
    13861 atctggtgcg cgcggctgaa ttttacgccc tgctgccggc aatcctcaaa gcccggctgt
    13921 aatcgctcaa ttcaaaaatt atcactcgat atcttatcaa ggagactgaa atgcaaactt
    13981 accttcacgg tcgtgacctg attggcgacc tcgatttctc gaaagaagaa gtcgaaaccg
    14041 ttttagacgt ggcatttgac ctcaagcgca agcgcgccct gggcgaaccc cacgcttacc
    14101 tgcgcgataa agtgctggcg atgctcttct tcttctccag cacccgcacc cgcggttcat
    14161 ttgaagccgg cattgcccag cttggcggcc atggcgcttt catcgaatcc tccaccaccc
    14221 agatcaagca cggtgatacc gccaaagaaa tcggcgaaat ctacggccgc tactttgatg
    14281 ccattgccat ccgccactgc gactggcaga ttggcaatgc ctacctcaat gaggttgccc
    14341 gcaactgccg cgtcccggtc ctcaacatgc agtgcgatgt ctatcatccc ttccagtgcc
    14401 tcgccgacct gatgaccatt gtggaaaaga agggccgtga cttgcgccgc aagaagatcg
    14461 tggtctcgtg ggcctacgcc gcttcctacc tcaaacccat ctccgtgccg cagtcactca
    14521 tcctccagat gccccgcttt ggcatggatg ttgtgctggc ctaccccaaa gaatttatgc
    14581 tcatgcccga aattgaagcc gaagcccagg cccaggcccg caagttcggc accggctttg
    14641 aaatcgttca cgatatggac gaaggctgcc gcgatgccga tgtcatctac gccaagagct
    14701 ggggccccct gctcaccacc tcagacgcgg cccagggcaa ggtgctgcaa gacagccaca
    14761 tcgactggat tatggatcag cgccgcctgc gcctggccaa agaagatgcc atctacatgc
    14821 accccctgcc cgccgaccgc aatatcgaag tgaccgatga agtgattgac ggccccaact
    14881 ctgtcgtgta tgatgaggcc gaaaatcgtc tgcacgttca gaaggccgtc atggcgctga
    14941 cgatgtaatt gatccaacct acccttttta tttggtttga ggagctttga atgactaaaa
    15001 aacgtgttgc tgttgtggcc gtgggtggca attcgctgat caaagacgaa aaacgtaagt
    15061 ccattcccga tcagtacgag gcctccgtcg agtccatgcg ccacattgtg gacatggtgg
    15121 agcagggctg ggaagttgtc gtcacgcacg gcaacggccc gcaggtcggc ttcatccacc
    15181 gccgcagcga gatctctcgc catgaactgc acgaggtgcc cctggattac tgcggcgccg
    15241 atacgcaggg ttccattggc tacatgttcg tccaggcact gcataatgaa ttccggctgc
    15301 gcggcatcaa gaaacatgcc gtcgccgtgg tcacgcagac ccttgtggac ccccatgatc
    15361 aggctttcca gcatcctacc aagccgattg gctcctttat ggacgaagcc acggctcacg
    15421 aacgcgccgc ccgcgaaggc tgggtggtgg tggaagatgc cggccgcggc tggcgccgtg
    15481 tggtggcctc tcccatcccg caggaaattg tcgaagccga tgccatccat aagctgatta
    15541 acgctggctt tatggtcgtc ggtgtgggcg gcggcggcat ccccgtggtc aagctggaca
    15601 acggcgacct gaaaggtgtt gaagccgtga ttgataagga cttcggctcc tcactgctgg
    15661 caaccatgat cggtgccaac ctgtttatca tcagcaccgc ggttgagaaa gttgccatca
    15721 acttcaacaa acccaaccag cagtggctcg accgcatgac tgtggctgag gccaaacaat
    15781 acattgccga aggccacttt gccaagggct ccatgctccc caagatccag gccattctgc
    15841 gcttcctcga acatgatggc ggcgaagccc tcatcaccaa ccccgaaaac atcggccggg
    15901 cgctgcgcgg cgaaaccggc acatggattg tcccgtagtg gtacaattaa ctggactgcg
    15961 gtccacgaag aggggcgggc ttatcaagcc cgccccacct ctaaactgaa aaccaaggag
    16021 gtgagcccgg cgcaaatcat ctcatggata cccagtcatc tgacacatcg ttcccgttca
    16081 ctttttcaat ccaataggag gagcaagatg agttccaaac gtttatggtt cgtggtgagc
    16141 ctgatgctgg tgctggctat ggcgctggca gcctgcaaga cccccacaac cgaattgacc
    16201 caacagcccg aagttgaaac cgaagaactg gtctttggta tgctgctggt tggtccctat
    16261 aatgaccacg gctacagcca ggcgcattat gacgccgggt tgtatgtgga agagaacttt
    16321 gatggcgctc gcatggtgta tgtggacaag gtgaaccccg ccgaccgccc cggcaccacc
    16381 gccgatcaat tagctgaaga actgctggcg aaaggcgcca cggtcatcat cttcaactca
    16441 gatgatatga aagatgctgc cgtgaccttt gcccaggcgc accccgaagt gatcgtgatc
    16501 catgcttctg gcgacaatgc ctggaaagac ggccagaact acaaagctct gcccaatctc
    16561 agcaacgtca tgggccggat ggaatttggc aagatgatgg ctggctgcgc cgccgccctc
    16621 accaccgaaa ccggtaaaat tggctacctc gggccgctaa tcaacgacga aacccgccgc
    16681 ctggcctcct cggcctacct cggcgcccgc tactgctacg agaaatatcg cggcgaaaac
    16741 cccgatgacc tcgaattcaa agtcacctgg attggcttct ggttcaacat ccccggcgtc
    16801 acctctgacc ccactcaggt cgccaacgac ttctatgaca agggttacga tgtcgtcatc
    16861 tcgggcattg ataccaccga agctctggtg caggccaagc agaaaactgc tgaaggcaag
    16921 aaagtctttg ccatccccta cgacttcatg ggcgcctgcg ccgaagccga aaatgtctgc
    16981 ctgggcgtgc cgtacttcaa ctggggcccg gcctacctga gcatgctcaa agctgccgat
    17041 gccggtaccc tgcagcccac ctgggatttt gttggcccgg attggaaaga catcaacaac
    17101 ctcgacacca gcgccattgg cttcatcaag ggcccggcgc tccctgccga tgccggcacc
    17161 aacctcgata agttcattgc tgaactggcg ggcggtctca atctgtggac cggcccgatc
    17221 aagctgcaag atggttccga atacctgaaa gctggcgaag ttgccaccga cttccaggtc
    17281 tggtacctgc cgcagctgct cgatggcatg gaaggccaga gcgttagcga gtaatccatc
    17341 atcacatctg gaggaaggcg gtttgccgcc ttcctccttt taccggaaca ctggcggtgc
    17401 ttatgaatgt cgaactgcga aacatccaca aacattttgg gaaagtccac gccaacaacg
    17461 atatcacgtt gaacatcccg gccggggtca ttcagggtat tctgggcgaa aatggcgccg
    17521 gtaaatcaac tttaatgaaa gtgctctccg gctatatcca cgccgattcg ggtgaaattt
    17581 tgctggacgg ccaacccgtg cgcatcacct cgcctgccga tgccattcgc tgcggcgtgg
    17641 gcatgctgca ccaggacccg ctggactttc cccccatgcg catcattgat aatttcatcc
    17701 ttggtcagcc gggtgggctg ctgcccaacc gccgccaggc cgagcgtgaa ttccacgaac
    17761 tgcgcacccg gttcgacttt aacatcaacc ccgatgccta tgtggacagc ctgaccgtgg
    17821 gcgagcgcca gcagcttgag attttgcggc tgctctggct gggtgcccgc gttctgattt
    17881 tggatgaacc caccacgggc atcagcgcag cgcagaaaac caagctcttt gccacgctgc
    17941 gtattttagc cgagcagggc aagacggcta tttttgtctc ccataaactg gaagacgttg
    18001 aagaactctg ccaccgcgtg gccgtgctcc agcgcggccg attggttggc gaggctgccc
    18061 cgccgtacgt caccgatgac ctggtgcgga tgatgttcgg caagggcatc aaactggcgg
    18121 accgtgccgc cctctctagc ggcaagtgcg gcctgagcgt caccgattta tccattgagg
    18181 atgtgcgcct gaatttacgc cacatctcgc tcgaggtgaa ctgcggcgag gtgattggtc
    18241 tggctggcat ggagggttcg ggtcagcgcc agttcttgcg ggtttgcggc ggtttgcagc
    18301 gcccggtggg cgggcacctg cacctcaacg ggcatgacct gaccggcaag ccctaccgca
    18361 attttctgga tgccggtgtg gcctatgtcc ccgcctcccg cctggaagag ggcctgatcc
    18421 caggtctgac gctcaccgag catttcatcc tcaccggtaa gcagcccggc ttttttatca
    18481 accgcgcggc ggctcaaaaa cgcactgaac agggcattga ggatttttca atccggggca
    18541 caccggccag ccaggttgag cggctctctg gtggcaatca gcagcgggcc ttgctggccc
    18601 tgcagcgcag cccgctcaac ctgctgctgc tcgaacaccc cacccgcggc ctggatattg
    18661 aatcctccat ctggatctgg ggcaagctca aagagcgctg ccgccagggc accagtatca
    18721 tctttatttc tgccgatctg gacgaaattt tgcactacag cgaccgcgtg ctggtctttt
    18781 ttggcggccg ggtgtccgag ccattggatg cggcctcgac ctcggtggac cagttgggcc
    18841 agctcatcgg cggaaaggga ttttagcgtt atgaaatcat tccgttggtc tacagttggt
    18901 tttcaagttc tggcggttgt gctggcgctg gccttcacct cgctggtgct gatgattgcc
    18961 ggcaccccac cgttggcggc ctatgccaat atcctcaacg gcgcgttcaa ctccatgaag
    19021 agcttcgccg atgtggccgt ggcctggatc ccactgctga ttgtcaccgc gggcctgctt
    19081 gtcaccttct ccgccgggct ttggaacatc ggcattgaag gccagatcat ggtcggcgcc
    19141 atcttcacca ccggcgcgct gcgcctgctg caagattcgg ccctgccctc ggcattgatt
    19201 atagtggcag gcttcctggc gggcatgctg ggcggggcgc tctgggctgc cctggccggg
    19261 ctgctcaaaa tctacggcgg cgtgaatgaa atctttggcg gcctgggcct caactttgtg
    19321 gccaccgccc tggccctctg gctgatcttt ggcccctgga agcgccccgg cgtcggctcg
    19381 atgagcggca cagaaccctt ccccgaggcg ctctggctgc cgctgctgcc cggcacccgg
    19441 ctcagcggct ggtcggtggc ggtggggctg gtgggcatca tcgtcatcta cttcctgctg
    19501 cagggcacat attttggcct gcggctgaag gccgtggggc gcaatgcccg ggcagccttc
    19561 ctgttgggca tccccaccag tatgtacggt ctgctggcgt ttctaatttg cggcgctctg
    19621 gcaggcgcag cgggtgcggt tcaggtcacg ggcgtctatc atcgcctgat cccgtccatt
    19681 tcatcgggct atggcttttt aggcctgatg gtcgctatgc tggttaatta tcaggccatc
    19741 tgggcggtgc tggtctcgct cttcttcgcc gccttgaata tcggcagtat ccaactgccc
    19801 attgtcctca aggtggactc gtcgcttgcc ggcgtgctgc agggcgcgct ggtgctcttc
    19861 ttcatgctga tggacggcgt ccggcggcgc ttccagccca aatcataagg agctgaacat
    19921 ggactctgcc tttctctctg gtttggctgg aattttagcc gcagcggcgc caatcttgtt
    19981 cgccgccatt ggcgaaactt tgacggaacg cgctggcgtg accaatctgt cagtcaacgg
    20041 cgccattatc ctgaccgcca tggtcggctt cgtggttgcc gtggagacca acagcaccgt
    20101 gctgggtttt ttggccgggg cggttgttgg ggcgctggtc accttgctgc tggcatttgc
    20161 caccatcacc ctcaagcagt cgcaggtggc ggtggggttt gtgctggccc tcctctgccg
    20221 tgacctggct tacttcctcg gcaaccccta catgggcgtt tctggtccca tcgtcagcca
    20281 cctgcccatc cctctgctgg ctgatattcc catcattggg caaatcttct tccgccataa
    20341 cctgccggtc tatgccagct atctgctggt ctttctggcc ttctggtggc tcttcaaaac
    20401 ccgccccggc ctgatgctgc aaggcatcgg cgagcgcccc gaagcagcct ttgcccgcgg
    20461 cgccaatgtc aaccggatgc gttacttcta caccgttctg ggcggcgccc tggtgggcat
    20521 tgccggaccg ctgtattcgc tcagcgtcaa ggctggctgg aagggcacca tttctggcct
    20581 ggacggcatc ggctggattg cgctggcgat caccatcttc ggcgcctgga accccatgcg
    20641 cgccgcactg ggcgcatact tcttcgcctt cttgcagtgg ctgggcctcg tgctgcagcc
    20701 cagtctgccg ggcatccctt cccaggtctt gcaggtggca cccttcccgt tgatgatcct
    20761 gaccctgctc ttggtcaata ttggcaatgc cgagtgggtg gatcgcgccc tggctgccat
    20821 gcccgaaaag acccgtcatt tcatctcacg cctgctgcgt gccatgcggt catcgccgcc
    20881 gggcgccctg ggcgtgcctt ttgataacga ataaacgacc atggcagctt ttttaggtta
    20941 tcgctgttcg ttgtgtcagg ccgaatatac gcccggtcag gtaacctata cctgccctcg
    21001 cgatggcggc aatctggatg tggtgctgga ttatgccgct ctgcggcaga aatatgaacc
    21061 cgccgatctt acttcccgca ccgaggaatc gctctggcgt tacttgccgc tgctgccggt
    21121 ggccgatccc gaggctgaag gcacgccgct gaaccgcgcc ggctggacgc cgctctacac
    21181 cccttcccgg ctggccgaat cgctggattt acgccaactc tggatcaagg acgagggccg
    21241 caatcccacg gcttctttca aagaccgggc cagcgctgtg gtggtggccc gcgcccggca
    21301 gttgcaggct gaaattgtag tcaccgcttc aacaggcaat gccggggctg ccctggcagg
    21361 catggccgcg gcagtgggcc agcctgcggt gatctttgcc ccacgcagcg cccctcccgc
    21421 caaggtggcg cagctgctgg tctatggcgc ccgcgtcatc ctggtggatg gcagctacga
    21481 ccaggctttt gacctttcgg tgctggccgc ccaggaattt ggctggtact gccgtaatac
    21541 gggctacaat cctttcacgc tggagggtaa aaagaccgcc gcctttgaaa tctgggagca
    21601 ggtcatcctc aacctcagcc cgtcatcccg cccgctgaca gtctttgtct cagtcggcga
    21661 tggcaacatc atctctggca ttcataaggg cttcaaagat ttgcaggccc tcggctggct
    21721 ggatcaaatg ccgcggttga tcggtgtgca ggccgaaggc tctgccgcag tggccaacgc
    21781 atttcgctct ggcgtcgaaa ccatcatccc ggttcaggcc caaaccctgg ccgacagcat
    21841 ctcggtagat ttaccccgtg atggcgtccg ggctgtgcgc gcagccacgc aaactggcgg
    21901 cgcttacctt accgtctcgg atgttgaaat tctgtccgcc attgccgaac tggggcgggt
    21961 gggaatcttt gctgaacccg ccgcggccac agcctatgct ggactgatga gcgccgtttc
    22021 gcaggaaatg gtgcggccag aagatccggt tctggtcatg aataccggca gcggcctcaa
    22081 agatgtgcgc gcagccatgc aggcggtcca ggcagccccg atcatcgagc cgaccatgcc
    22141 cgccctcagg cggctgctgg caatttagcc agccctttgg ggagcgtcga tctccagcgt
    22201 gcgactttag ccgtacgctg gtttactgct taaacactgc aacgccggtg agcggccggc
    22261 gttgcaagcg gagagagggg agcgggagga tgggataaat tactgtcggg tcggcgggtc
    22321 gctagcagga gttcgcgaaa caggctgacc agaaagggaa tggtatgagg gttctgggcg
    22381 ggaactggta gccgtggaga gcgggcgggg gtaaggccgg g
//
LOCUS       DAOPVS010000021        22288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2331_length_22288_cov_55.662349, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000021 DAOPVS010000000
VERSION     DAOPVS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 22288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22288
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_22288_cov_55.662349"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="PKL11_03600"
     CDS             complement(<1..2285)
                     /locus_tag="PKL11_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12718.1"
                     /translation="MATLLAQILKMLTTPPGNLLYHLVLAFSIIAALQGFLFKRNKPE
                     PVAKRLALGLVVILLAQLALFLVSGLAWQKVVSPAHLLPPLDRWLTVASLLWIVWIWC
                     FPTPCRWGDILNTVLNLGAIGLLFFEIYAWSQAAQADPTATFNRGMLDWYWQMFASAI
                     LIGGLLILFIRRPQEWGLGVGFMGLMLAGVMAHLLIETEAGNFAGFVRLAQLCTYPLL
                     PGLVRRLSPDTEAAAEPVRSEDTVQSQRRGQTDPRIIQAWLDISNQTEPLKVGELLAE
                     AISRTMVADLAFISSVPDPFGQVNFICGYDLIREEALPGFSLPQNRIPLLASSIQRNK
                     PLKLSPQGNPPQDLKAICEAVNVRDPGHVLVIPLQHGNQVVGSVMIMSPYSNYGWSME
                     DQANLQANSGRMAAVLNRALEQNALGASGENSQQVLEAARTQILALEQQVQVLRSSAE
                     TNPEVESLLALRAEAQETIRRLDAENESLRTALEDAAHAVRPTSEVEQQLESELRMTL
                     EEMARLQNGMAQANMKIMDLEKELNAGGGITGQERDAISGSIHELRQPLSSILGYTEL
                     LLGESIGILGTQQKKFLERTKHASEQMRQLLDQLMGIVNRQGELSELTPEAIDLAAVI
                     DQTISDTSAQLREKNLTLRVGMPEVLPRIFADREALQQIMGHLLQNAGSASPQEGIIR
                     LNIQINQDIDETTYLLIQVTDSGGGIPPEDLPRIFARQASADGSETTIAGIGENTAGL
                     AIAKALVEAHGGRIWVESEIG"
     gene            2389..2472
                     /locus_tag="PKL11_03605"
     tRNA            2389..2472
                     /locus_tag="PKL11_03605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2423..2425,aa:Leu,seq:cag)
     gene            2543..2920
                     /locus_tag="PKL11_03610"
     CDS             2543..2920
                     /locus_tag="PKL11_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12719.1"
                     /translation="MNWVKTNLRRILIVGAVVVLLLLMMDLNNRVVALNRLTNQRDDM
                     RTEISQYSATATVLYTQIAFATSEAAVDIWARAKAHYILPGDVLVVPLPPPGATPEPV
                     FFPTPTVMPVENYQVWWALIFGE"
     gene            complement(2936..>6757)
                     /locus_tag="PKL11_03615"
     CDS             complement(2936..>6757)
                     /locus_tag="PKL11_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12720.1"
                     /translation="VGQLAGMPEWRQFLGLSEALLAEPNAAAQAALMRKTLETLLGCR
                     GEVWFVPPAYPLPGEPDIRLLPDDAAPEIVRQVYQTHEKDCSAENGAVVPCDEGKPPR
                     QLALPLLSRDYLLAILLVERQEGPPLRPDEIAFLEGLCAHAAVALQISRQERLKNWRY
                     EQLTLVRSVSAQISNLPDLNELCRRVTTLIEQTFHYYSVSIFTMSEDGTELIFQAGSS
                     AATHPVENRPHFRVKLGQGIVGTVGKTGEEILAKDVRTEPHYRAVSILPETRSEIALP
                     LKAGESVVGVLDIQSNRIDGFHEIDMIVLRALANNVALAVQGTRLYSAIRWRAEQMEA
                     IFEVSRALNSILDMEVLLDQVVEVIHKRFGYPYVHLFTTHLGRRTVEFVAGSGQRSEM
                     ICEKCLQYSIDDPLGIIPWVARAGEPRLVNDISKDPLYRPFELTSDSTQSELCVPLKF
                     GDEVLGVLDIQSETVNTFNTDDLTLFQALASSIAVTIRNATMYRTERWRRQVADSFRE
                     VASLIASNVTLEDLLEKVLSELERNLPTEAAVIWLLEDPQNEGYGALRPIAAHGVEIA
                     RLLEVAREDSEVDDQLHATLISSEPQIRQADEAYGPLGKAMGFPQDYSSIAIPLRVGE
                     TALGILALAHESPGRYGSEARAMITTFAGYAAVAIQNARLYSASQEQAWIATILLQVS
                     EASQAINNISELLETMVRLTPLLAGIQQCAFFLWDETLQVFSLEAEYGLNISRAEPLL
                     LEPELPALARLRESGGMVFIYDAASELALPAAGTADETTTLVLMPLQARGEILGAFLV
                     GHHADLNALGNSMLDQHSMAILQGIVHQTAVAVENIRLLETRQEEAYVTAVLLQVAQA
                     VVSQNELDDVLEAIVHLIPILVGMDACAVYLWDPETQSHRATKAYATDEDSIQSILSG
                     DYRLGEFALLDAAIKAQDLIVCPEGEFSVPPGDWPNLVCLQPGQKPSPRQAARGHWLV
                     GYPLAVKGEHFGVLVMQEDGVPAGFQDRRVELITGVAQQVALAIQNERLKAEMVARER
                     LEREIQLAREIQQTFLPSHLPDLPGWELDIRWQTARQVGGDFYDIIRLSKGRLGLVIA
                     DVSDKGLPAALYMTVARTLIRAMQGVESPARALERVNNLMLVDSKNGLFVTAIYAILS
                     LEKGTLTYANAGHNRPLLLRAHNDAIEQLERGGMAMGVMEDIKYDEHVITIEPNDSLL
                     FFTDGLTDATSPTEEMFGEERILETIAGVRGKDVCQLLETLDHAVSEFRQNAAASDDL
                     TLLAIRRVTEE"
     assembly_gap    6758..6857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7025..7360)
                     /locus_tag="PKL11_03620"
     CDS             complement(7025..7360)
                     /locus_tag="PKL11_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNZ12721.1"
                     /translation="MEIVHTPFKHCDVIKVQGRIDSATAPKFAEAINAITEQGRYRIV
                     IDMSELVFISSAGLRVLISTQKTCKRYNRGELTLAEVPANIKSTLELAGFNILFKFYD
                     SVLTAVGNF"
     gene            7601..8347
                     /locus_tag="PKL11_03625"
     CDS             7601..8347
                     /locus_tag="PKL11_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ12722.1"
                     /translation="MSGKENPQNIIEGYKKRQQLMPFLVGGAAVLLVAVGIIILIAWL
                     TGDNKPSGFTLFATKTPTPTSTFTATPTVPSPTPTVTNTPTETPTITLTPTPTGPREY
                     TVQANDNCWDIAVAQNVELSVLLAINGFTPGTCPITEGQVILIPAPDQTLPTATPLPP
                     DLPAGTRIEYYVGAGETLGVIAEKFNTTVDAIVAIRDNGLTLDTINTIYEGQKLIIPV
                     NLVTPTPTKQPTLTPLPATATTAPTATPTP"
     gene            complement(8360..9496)
                     /gene="queG"
                     /locus_tag="PKL11_03630"
     CDS             complement(8360..9496)
                     /gene="queG"
                     /locus_tag="PKL11_03630"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNZ12723.1"
                     /translation="MNGLRQVLQDCAEARGLHLLGISPAQMPTHIEVYEKWIEAGKQA
                     EMGYLARPDAVVKRRNPAELLPGLRSIAVVGLRYAAPGPAVLPGDPTRGQVAAYAWGK
                     DYHSVIPEKLQWLAEDFQKASRKTFNWRIYTDTGPLLERDLAQQAGLGWIGKNTCLIS
                     SAWGSFILLGELLFDTEIEADAPFTADRCGTCTRCIEACPTGCIGADRTLDARRCISY
                     LTIEHKGSIPPELRPALGNRVFGCDICQQVCPWNRKWLNTAGDAALEPRAETATPALI
                     EALGLSATEFNLRYKDSPILRAKRRGYLRNVATALGNAGLTGSIPALENVLLNEPEPL
                     IRGAAAWALGQIGSTTSQQILWAAQKMEGEPEVQMEIRAALQKM"
     gene            complement(9493..10974)
                     /locus_tag="PKL11_03635"
     CDS             complement(9493..10974)
                     /locus_tag="PKL11_03635"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNZ12724.1"
                     /translation="MKRVWRILWLSALLLGACTQTPPPADPTLPASTEAAPTVDFIET
                     PTLEPTALPSVTPAAPQPITYTLNVQWDFGYRQVVVQQDIGYTNNSTEPITQLVLMAE
                     PNRWKGAFELEALTGADGQNYPNAVLDSNQLTIPLDTPLMPGQRIALQAHYTIVLPEI
                     PPPSESYRPVPFGYTVNQSNLVDWYLYFPPYEAGRGWLAHPAWYYGEHQVYELADFDI
                     TLDVSNAPEGLVLAASSPAEQDGSRYHYTMKDCRSFAISGSPVYTVFEKQVGNITVLS
                     YSLFPFSHPAAEAALEATSEAVSLYGELFTPYTRTTLSVVEADFLDGMEYDGLYFLSR
                     GFYSLYEGTDRGYLTMIAVHETSHQWWFGSVGNDQGLEPWLDESLATYCERLYYEQSG
                     PEAVNWWWAYRVDFYNPAGWINRGIYDYGGYLPYRNAVYLRGAQFFEDLRTAMGDEAF
                     FAFLKDYAETLQNQTADREAFFEIAARHTDADLSSLLAEYFMP"
     gene            complement(10986..11696)
                     /locus_tag="PKL11_03640"
     CDS             complement(10986..11696)
                     /locus_tag="PKL11_03640"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNZ12725.1"
                     /translation="MTSIKKILIGALMLVSLLTLAACGKVEPTQSADEVRTQGAQTVQ
                     AWMTATAAMMPSPTATWTLAPTNTPQPPTATVGATATGATATLAVTAPVVTPPAGGTS
                     DRGLWISQSPTDNTSFAPGADFAMTWHIKNTGTTTWTTGYQLRYYAGSGGRMSATDIS
                     FPKEVKPEEEVDLTLNMKAPTTGGTYDSIWVLTNANGANFYSVTITIKVEGAASTATA
                     TLESTATNTPEPTLTPTP"
     gene            complement(11794..12873)
                     /locus_tag="PKL11_03645"
     CDS             complement(11794..12873)
                     /locus_tag="PKL11_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNZ12726.1"
                     /translation="MKPYWYLALLIVLLISLAGCKSGDTAGDLTATPPLAEAGEQMPE
                     GTPPPAVTPTPEYTHDPNPLRLVFPTPGADPSFLWRPPLGEAPWALGAKDHFYFSRPI
                     AVDEVNWPLADYRYGYVFPDSTILHTGIDIDAPPGTPVIAAASGRVVWAGYGLLHGDD
                     DPEDPYGLAVMIRHDFGFEGHKLQTVYAHMDEINVILGQRVETGDTLGLVGNTGNTTG
                     PHLHFEVRLESGERYYTHRNPELWLTPPQGMGVLVGRLLDGTGSFLSGVEVTVKSQTT
                     GRKWIILSYAPWLNSDEYYRENLVLSDLPVGDYTLSFTQRGTDYTYDFSIHNGAVTYF
                     VFRDGRGFSNALPSQDAPEGWIPIP"
     gene            complement(12927..14837)
                     /locus_tag="PKL11_03650"
     CDS             complement(12927..14837)
                     /locus_tag="PKL11_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12727.1"
                     /translation="MGKLKAFFTRKRVRIILMVLAGLLLVGAIVSAFIWPPVEPVVSP
                     TVTRSVTPTGEASPTAAAVLENPPVVEITPISNEQAAGLRDQGFVFLALTDGLYTHLF
                     AYHPEYLPLVRLTNTPWDDADPTVSPDGKVLAYASRQNGYWDIYLLNLETGEQTRVTD
                     TPAYDGAPTWSPDGEWLAYETYTNDNLEIYIQSISDPTQDPIQLTSDPAADFSPAWSP
                     QGREIAFTSTRNGNQEIWTARLDSQGERFTNLSQTPGVQEDHPAWSPDGAWLAWSADG
                     QGTRQIMVWDTAAPGNPARALGSGDWPAWSPGSDAILTAVVGPNQTGLTAYALDGAKM
                     ILPFLDLPGAVRGLEWKALSQPADDPLPPLLQINLPAPETLYTPEISLTQMAPAGRYG
                     IVGLPDVTAPYPYLHDQVDEAYSALRAGVGVTAGWDFLLSLENAYKPITYPVSPGVTQ
                     DWLYTGRAFALNPIPLDAGWLVAVREEIGSQTYWRVYIKARYQDGSQGTPLQQMIWDL
                     NARYSGDPLAYEAGGQVGPVPAGYWIDFTDLAARYGWQRLSAFRNWPTYYPATRFNQF
                     VMTGGLTWEAAMDQLYPVEALITPTYVPTYTPEPTATPRPTRGPTQATPTLTPTATLR
                     PTWTLPPPTPNP"
     gene            complement(14848..15762)
                     /locus_tag="PKL11_03655"
     CDS             complement(14848..15762)
                     /locus_tag="PKL11_03655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ12728.1"
                     /translation="MKRPLRTDKLLRLNHRITHGGWVLLLALFMLAGCTPTVGDSALP
                     TVTATTAIESPTPLPNPPVKPATETPTLMSPTETPEPPVFSGLCSPLEDITLAELPEI
                     LKNPFEMPSPGRDDGHHGADYAYYSRGEHSQMLGLGVMSVLDGETVAAIQDRPPYGNM
                     IIVETPLERLPESWLEMLALPEPGMIATPDGRVNCPALPVTPEWDNSQRSLYVLYAHL
                     NQPALSTVGEAVTCGETIGEVGTTGASVNPHLHIEVRVGPAGANFPVMAHYANNATPD
                     EMGYYCLWRLSGIFQPIDPALLLRDDLK"
     gene            complement(15737..16093)
                     /locus_tag="PKL11_03660"
     CDS             complement(15737..16093)
                     /locus_tag="PKL11_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12729.1"
                     /translation="MPHFALQPPDEDALAPADVRLVDLAVEPYPDGRRVRILVDITPF
                     QQPPNLEIRISAANGEVVSVTHIIETAVEQLKFTMHIRSAEAVGPFTAAVRLYYPDLE
                     CVDERQVNFETPAANG"
     gene            complement(16106..17425)
                     /locus_tag="PKL11_03665"
     CDS             complement(16106..17425)
                     /locus_tag="PKL11_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNZ12730.1"
                     /translation="MRNYPEIGIQIADILLPKPGTDLTKWAVIACDQFTSQPEYWQQV
                     EDMVGSAPSTFRLTLPEVYLETPEEPRRIESTQQAMQAYLAKDLLTAYEGLIYVERTV
                     EGRTRRGLMLALDLERYDYNKGSQTLIRATEGTILERIPPRVRIRQGAALELPHILVL
                     IDDPEKTVIEPMAAQRDHLVPVYDFELMLGSGHLRGYRVTDLAIEKQVVGALEKLADP
                     QKFAQRYGVGADKGVLLFAMGDGNHSLATAKAIWEKLKPTVGMNHPARYALVEIENVH
                     DEGLEFEPIHRVLFGVKQNVVEAMTAFYGGAIHFQSCKSAAEMVEVVDGQQGVEQAVG
                     VISSAGYQVAWITKPTSNLPVGTLQVFLDGWLKNGGAEKIDYVHGEDVVCELGAQPGK
                     LGFYLPGMPKSDLFKTVILDGALPRKTFSMGEAKEKRFYMEARKITA"
     gene            complement(17484..17768)
                     /locus_tag="PKL11_03670"
     CDS             complement(17484..17768)
                     /locus_tag="PKL11_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNZ12731.1"
                     /translation="MKIQDTETKTRLVQRLRRIEGQVRGVQAMLNEERDCREIMQQLT
                     AIHAAVQATSRTFLQDYTAACLTEMDLDPDSRARQANMVAEMIALLDKTP"
     gene            complement(17821..18030)
                     /locus_tag="PKL11_03675"
     CDS             complement(17821..18030)
                     /locus_tag="PKL11_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ12732.1"
                     /translation="MPLYEYRCNQCGETFEKMVRFSEVDLSPVCPKCASTDTKKKVSM
                     AAAFGGGTSGASSGGSSCGSHGGFG"
     gene            complement(18109..18717)
                     /locus_tag="PKL11_03680"
     CDS             complement(18109..18717)
                     /locus_tag="PKL11_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HNZ12733.1"
                     /translation="MDIFTLLDRLQAIARDGLHYAANPFDRERYERLMDLACETYSEA
                     LEAPTSEIKKRFLGEIGTITPKVGTDAAIFNSRGEILLMDRADGSGWCLPCGFVEPNE
                     TPVEGIIREVREETGLEVQINRLVGVFTRKPSAQYGPHTTLSIVHLCDVIGGQLALSH
                     EGLGLQYWDIGAVPRWHTTHEKLARAAYKIWAADEFIQPICE"
     gene            complement(18797..20182)
                     /locus_tag="PKL11_03685"
     CDS             complement(18797..20182)
                     /locus_tag="PKL11_03685"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNZ12734.1"
                     /translation="MKEKILQTLRELRAYAISKNVTATFFYHEEDSYLMRFANSAISL
                     NTNEHLIRLEITAYEGRKRASYELITHLGKLEEMKQGVDIAAEMVKHAQPLNYEPTIP
                     QYTETFIDESAYDAELAKLSNEARLAYFNAACDGLETEDIKLGGIFSNGTNVTAQINT
                     RSEHAQYFVTSDAQISVVLSHTKLKWEVIAERSAQQKSDLNPKSVRSDLSFLLDRYQH
                     DAPQQLPVGSYDIVFGSAAIADLMNFMNYVGFNGGTMKRGYSFLSEAQIGTKVFSEQF
                     SLTDDPTRRETYPFRRDIMGMPRKSFPLVVNGVFKGFTWYQDDADEFGAQPTGHTVMH
                     KSLVINGGTMDVSTLAELAAMPRDKDILYIPFLHYMNLVNPSRGLFTGSSRFGALLLK
                     KDGSIVVPYNVRVTQSLLDIFGDKVAWISKTQVVSNQSSSYGARNPTAMIVPAFMRVN
                     GLEISHSNSSY"
     gene            complement(20185..21639)
                     /locus_tag="PKL11_03690"
     CDS             complement(20185..21639)
                     /locus_tag="PKL11_03690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNZ12735.1"
                     /translation="MQKQLQAQIERLKQAGATYADVRWYPVEETSELVMWNGNLKNAA
                     ASRQSGLGIRVLYRGAWGFSASSDLSNLSALFDQALSNARIAAQRVSFPVRLAEKDAI
                     QASFNSPNRIDPFGVPLTDKIAFMQVMDEKLNRPGVMQRICALTFVRKQIVFFDSEGS
                     EIEKNIIEVFPEIQVMGVDPDGVSQVRKFSPSRMGTTRGWETVDMQMFSENADRIVRE
                     LNEVLAAESCPKEDRSVILLPGIMFLQTHETIGHALELDRILGYELAFAGGSFVTLDD
                     FGKLRYGSPKLTARADATLTNSPGSFGYDDDGVPAQNNLLIDKGILVGAITGRQMVEE
                     ANAKARKQIFDGSSGANRATAFYRVPIERMTNINIDPGEDGTLDDIVRNTERGIILDG
                     EKSWSIGSNREQFHFATEIGWLVDDGKVTRVVKNSTYRGETLKFYNSLSAVGDQSTWE
                     VRYVDNCGKGQPNQIMQLGHGVPVCRFDNVRIGE"
     gene            complement(21815..>22288)
                     /locus_tag="PKL11_03695"
     CDS             complement(21815..>22288)
                     /locus_tag="PKL11_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12736.1"
                     /translation="CPCGNFGNPMKACTCSSATVTKYQKRISGPLMDRIDIHIQTPQV
                     DYEKLSDARMGEASATIRARVEAARQIQRERLATAGLSSNTEMRPAEVRQFCALDDTG
                     RSLMKSAMQQLELSARAYHRILKLARTIADLASSEVILPVHLAEALQYRPRVDFG"
BASE COUNT         4673 a         6866 c         5702 g         4947 t
ORIGIN      
        1 tgtccaattt cgctctcaac ccagatgcgc ccgccgtgcg cctcaaccag cgctttggca
       61 attgccagcc ccgcggtgtt ttcgccaata ccagcgatgg tcgtttcact gccatcagca
      121 ctggcctgac gggcaaaaat ccgcggcaaa tcctctggcg gaatgccccc accgctgtcg
      181 gtaacctgaa tcaacagata ggttgtttca tcaatatcct ggttgatctg aatattgagc
      241 cggataatcc cttcttgtgg cgatgcgctc cctgcatttt gcagcaagtg tcccatgatt
      301 tgctgcagtg cttcgcggtc agcaaaaatc ctgggcagca cttcaggcat gcccacgcgc
      361 agagtcaagt tcttttcccg cagttgggcg cttgtatcgc tgatcgtctg gtcaattacg
      421 gcagccaggt caatcgcttc aggcgtcagc tccgagagct cgccctgtcg gttaacgatc
      481 cccatcagtt gatccaataa ctgccgcatt tgttcagagg cgtgcttggt gcgttccagg
      541 aacttcttct gctgcgttcc cagaatgcca atcgactcac ccaaaagcag ctctgtgtac
      601 cccaaaatcg atgaaagcgg ctgccgcagt tcgtgaatac tgcccgaaat cgcgtcacgt
      661 tcctgcccgg taatcccacc gcctgcattc agttcttttt ccagatccat gatcttcata
      721 ttggcttgcg ccatgccatt ctgcagccgg gccatttctt ccaatgtcat ccgcagttca
      781 gattctaact gctgctcaac ctcggaggta ggccgcacgg catgggccgc atcttccagc
      841 gcggtacgca ggctctcatt ctcagcatcc agccgccgga tggtctcctg tgcttcggcg
      901 cgcagcgcca gcagtgattc aacctccggg ttggtctcgg cgctgctccg cagcacctgc
      961 acctgctgct ccagagccag aatttgcgtt cgcgcggctt ccagcacctg ctgcgagttt
     1021 tcgccgctgg cgccgagcgc attctgttcc agtgcccggt tcaaaactgc cgccatgcgc
     1081 ccgctgttag cctgcaagtt ggcctgatct tccatgctcc agccgtaatt ggaatatggc
     1141 gacataatca tcacgctccc caccacctga ttgccgtgct ggagcggtat caccagcaca
     1201 tgccccgggt cgcgtacatt caccgcctcg cagatagcct tcaggtcctg cggcgggttg
     1261 ccttgtggcg acagctttaa tggcttattg cgctgaatac tggaggccag cagcggaatc
     1321 cggttttgcg gcagggaaaa ccctggcagc gcttcctcgc ggatcaaatc gtatccgcag
     1381 ataaaattaa cctgcccaaa ggggtctggc accgatgaga taaacgccag gtccgccacc
     1441 atcgtccgag agatggcttc agccagcagt tcgccaactt tcagcggctc agtctgattc
     1501 gatatatcca gccaggcctg gatgattctt gggtccgtct gaccacggcg ttgcgactga
     1561 acggtatcct cgctgcgcac gggctcggct gctgcctctg tatctggcga taaacgccgc
     1621 accagtcccg gcagcagcgg ataagtgcat aactgcgcca gccgcacaaa cccggcaaaa
     1681 ttacccgcct cagtttcaat caacaggtgc gccatcacac ccgccagcat caaccccata
     1741 aacccaacac ccagccccca ttcctgtggc cgccggatga acaaaatcag cagtccacca
     1801 atgaggattg cgctggcaaa catttgccag taccagtcca gcatgccccg gttaaaggtg
     1861 gcggttgggt cggcctgcgc cgcctgtgac caggcataaa tctcaaagaa taataaccca
     1921 attgccccca ggttcaggac ggtattcaga atatcgcccc accggcaggg ggttggaaag
     1981 caccagatcc atacgatcca cagcaggctc gccacggtca accagcggtc aagcggcggc
     2041 agcaaatgcg ccggactgac caccttctgc caggccaggc cgctgaccag gaacagcgcc
     2101 aactgggcaa gcagaatcac cacaagtccc agggctaagc gtttggccac cggctcaggc
     2161 ttgtttcgct tgaacaggaa cccctgcagc gcagcaatga ttgagaatgc cagtaccaga
     2221 tggtacagta aattccccgg cggcgtggta agcattttga gaatttgagc aagtaatgta
     2281 gccataattt taatgattat agcacgggta tgaaactcgg ttgctttttt gaacccctga
     2341 aaggttgaca aatctgtaaa ttccggtaaa atactcctcg caagttgagc cgaagtggcg
     2401 gaattggcag acgcgctacg ttcagggcgt agtgggatta tcctcgtgag ggttcaaatc
     2461 cctccttcgg cacctcaaac cccgtgcaaa cggggttttt gttttcttgt cttgaaatta
     2521 atttgggtgt atgattaata ccatgaactg ggttaaaaca aatctccgcc gcattttaat
     2581 cgttggggca gtggttgtcc tgctcctcct gatgatggac ttaaataacc gggtggttgc
     2641 tctcaaccgc ttaaccaatc agcgagatga catgcggact gaaatctccc agtatagcgc
     2701 gacggcgact gtcctatata cccagatcgc ctttgcgact tccgaagcag ctgtcgatat
     2761 ctgggctcgc gccaaggcgc attacatcct gccgggggac gtactggttg tgcccttgcc
     2821 accgcccggc gccacacctg agcccgtctt ctttcccacc cccacggtga tgcctgtcga
     2881 aaattatcag gtttggtggg cgctaatctt cggcgaataa atcggttcgg caaacctatt
     2941 cttcagtcac tcggcggatg gctaacagcg tcaggtcgtc cgatgccgcc gcgttctgcc
     3001 tgaattcgct caccgcatga tccagtgtct ccagcagttg gcacacatct tttccgcgta
     3061 ccccggcaat cgtctccagg atccgctctt cgccgaacat ctcctcagta ggtgacgtgg
     3121 catcggtcag tccatccgta aaaaacagca aagagtcatt gggttcaatg gtgatgacat
     3181 gttcatcgta cttgatatct tccatcaccc ccatcgccat gccgccgcgt tccaattgct
     3241 caatcgcgtc gttgtgcgcc cgcagcagca gggggcggtt atgccccgca ttggcatacg
     3301 tcagcgttcc cttttccagc gataaaatcg cataaattgc cgtcacaaac aacccgttct
     3361 ttgaatccac cagcatcagg ttgttcaccc gttccagcgc ccgcgccggt gattcgaccc
     3421 cctgcatcgc ccggatcaac gtccgcgcca ccgtcatata gagcgccgcg ggcagcccct
     3481 tatccgatac atcggcaatc accaatccca accggccctt acttagccgg atgatatcat
     3541 aaaagtcgcc gcccacctgt cgggctgtct gccagcggat atccagctcc catccgggca
     3601 ggtctggtaa atgcgagggc aaaaaggtct gctgaatttc tcgcgccagt tgaatttctc
     3661 gttccagtcg ctcgcgcgcc accatttcag ccttcagccg ctcgttttga atggccagcg
     3721 ccacctgctg ggccacacca gtgatcaact caacccgccg gtcttgaaat cctgccggca
     3781 ccccatcttc ctgcattacc agcacgccga aatgctcgcc ctttacagcc agcggatatc
     3841 ccaccagcca gtgccctcgg gccgcctgac ggggcgacgg cttttgccca ggctgcaggc
     3901 agaccaggtt cggccaatct cccggcggca ctgagaattc accctccggg cacacaatta
     3961 aatcttgtgc cttgatcgcc gcatcgagca gtgcaaactc acccaggcgg taatctcccg
     4021 aaaggatgct ttgaatggag tcctcgtctg tggcataggc cttggttgcc cgatgcgact
     4081 gcgtttccgg gtcccacaaa tacaccgcac aggcatccat ccctaccagg atggggatca
     4141 ggtgaacaat cgcttccagc acgtcgtcca gctcattttg actgaccacc gcctgggcca
     4201 cctgcaaaag caccgcggtg acgtaggcct cctcctgccg ggtttccagc aggcgaatgt
     4261 tttcaaccgc caccgccgtc tgatgcacaa tcccctgcag aattgccatg ctgtgctgat
     4321 ccagcatcga attgcccagg gcattcagat cggcatggtg cccgaccaga aatgccccca
     4381 gaatttcgcc gcgcgcctgt aatggcatca gcaccagcgt ggtagtctcg tctgctgtac
     4441 cggcagccgg cagcgccagt tcgctcgcgg catcataaat aaagaccatc ccgccgcttt
     4501 ctcgcagccg ggccaaagct ggcagttctg gctctaagag cagcggctcg gcccgcgaaa
     4561 tatttaatcc atattccgcc tcaagcgaaa acacctgcag cgtctcgtcc cacaggaaga
     4621 aagcgcattg ctgaattccc gccagcagcg gggttaaccg caccatcgtc tccagcagct
     4681 cgctgatatt gttgatcgcc tgggaggctt ccgaaacctg cagcaagata gtcgcaatcc
     4741 aggcctgttc ttgcgaggct gaatacaggc gcgcgttctg aatggcgacg gccgcataac
     4801 ccgcaaatgt ggtgatcatt gcccgtgctt cgctgccgta gcgcccgggg ctttcgtgtg
     4861 ccagcgccag aatccccagc gcggtttcac ccacccgcag cgggatcgca atcgaagaat
     4921 aatcctgcgg gaaccccatg gctttcccca acggcccata ggcctcatcg gcctgccgga
     4981 tttgcggctc agacgagatc aacgtcgcgt ggagctgatc atccacctca ctgtcttcgc
     5041 gtgcaacttc taatagccgc gcaatctcca ccccgtgcgc cgcaatcggg cgtaaggccc
     5101 catacccttc attctgagga tcttccaaca gccagataac cgccgcctca gttggcaggt
     5161 tgcgctccaa ttcgctcagc actttttcca gcagatcttc cagcgttaca ttggaggcaa
     5221 tcagactggc cacctcccgg aagctgtcag ccacctgccg ccgccagcgc tcggtccgat
     5281 acatggtcgc gttgcgaatc gtcaccgcaa tgctcgacgc cagcgcctgg aacagagtca
     5341 aatcgtccgt gttgaacgta ttgacagttt cgctttggat atccagaacc cccagcacct
     5401 catcgccaaa ctttaaaggc acacacagtt cagattgcgt tgaatccgag gtcagttcaa
     5461 atggccgata gagcgggtct ttggagatgt cgttcaccag ccgcggctct ccagcgcgtg
     5521 ccacccacgg gatgatgccc aaggggtcat caatactgta ttgcaaacat ttctcgcaga
     5581 tcatttcact gcgctgtccg ctgcccgcca caaattccac cgtccgccgc cccagatgag
     5641 tggtgaacag atgtacatac ggatacccga accgcttgtg aatgacctca acaacctggt
     5701 cgagcagcac ttccatgtcc agaatcgagt ttagcgcccg gcttacctca aatatggctt
     5761 ccatctgttc tgcccgccat cggatggcgc tgtacagccg ggttccctgc actgccagcg
     5821 ccacattatt tgccagcgcc cgcaaaacaa tcatgtcaat ctcatggaac ccgtcaatcc
     5881 ggttggattg aatgtccagc acccccacca cactctcacc ggctttcagt ggcagggcaa
     5941 tttcagagcg cgtctcaggg aggatcgaaa cagcccgata atgcggctcg gtacgcacat
     6001 ccttcgccag tatctcttcg ccagtttttc ctaccgtccc cacaatgcct tgccccagct
     6061 ttacccgaaa atgtggtctg ttttctaccg ggtgggtagc cgcactgctc ccggcctgaa
     6121 agatcagttc agtcccgtct tcactcatgg tgaagatgga cacagagtaa tagtgaaaag
     6181 tctgctcaat gagcgtcgtt acccgccggc agagttcatt caaatcgggt aagttggaaa
     6241 tttgcgcgct gactgagcgt accagggtta gctgttcgta ccgccagttt ttcagtcgct
     6301 cctggcggga gatttgcagt gcaaccgccg catgagcgca cagcccttcc aaaaacgcaa
     6361 tttcatcggg tctcagcggc ggaccttctt gccgttccac caacaaaatc gccagcagat
     6421 aatcgcgtga aagcagcggc agcgccaact ggcgcggggg cttcccttca tcgcacggaa
     6481 ccaccgctcc gttctctgcc gagcagtctt tttcgtgggt ctgatacact tgccgaacaa
     6541 tttccggggc tgcgtcatcg ggcagcagcc ggatatcggg ctctcccggc aggggatagg
     6601 ccggtggcac aaaccagact tcgcctctac aacccagcag ggtttctagt gtcttgcgca
     6661 tcaaggcagc ctgcgcggcc gcattgggct cagccaaaag cgcttcgctc aaaccgagaa
     6721 actgccgcca ctccggcatt ccggcaagct gtcccacnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnntgc tcaatgatat tcgagcaggc ctcatccact gcggtttcca
     6901 cagaatacac cgcaaatgaa ctcaacccgg catcctgggc ggcctggcgg acgatagcag
     6961 caatctgttc cagactatcg tagcgtcctg gaaacgtgta ggttgtcatg ctctggcaaa
     7021 aggcttagaa gttgcctacc gcagtcagca ccgaatcata gaacttgaac aggatattga
     7081 aacctgccag ttccagggtg ctcttaatgt tcgcgggaac ttccgccagg gtcagttcgc
     7141 cccggttata gcgtttgcag gtcttctgag tgctaatcaa cacccgcagg cctgcgctgg
     7201 aaataaacac cagctcgctc atatcaatca caatccgata acgtccctgc tcagtgatcg
     7261 cattgatcgc ctcggcaaac ttgggggcag tggcgctgtc aatgcggccc tggactttga
     7321 tgacgtcaca atgtttgaat ggggtatgaa caatctccat gaaaagcctc caatgcaatt
     7381 tgaaaacaat cgggaatgtg caacaaaacg attatatcat acgccttgcc tttcaaaaca
     7441 accgaatggc cctttatttc acccaatttt cagattgctc caaccctcgc cgaatccttg
     7501 actttcgtag tagaatactc atgaacgttg tttatcaacg tgaatctcat ggttttcggg
     7561 caatccggcc cgaccaatca tcataacaaa ggatatgcca atgagtggaa aagagaatcc
     7621 acaaaacatc atcgagggtt ataagaaacg ccagcagctc atgccgttcc tggtgggcgg
     7681 cgcggcggtg ctgcttgtag ccgtggggat tatcatcctc atcgcctggc tgacgggtga
     7741 caataagcct tctggcttta ccctgtttgc caccaaaaca cccaccccaa ccagcacatt
     7801 cacggctacc cccacggtgc cctcacccac ccccacggtc accaataccc ccaccgaaac
     7861 ccccaccatc acgttgacac ccacacccac cggcccgcgg gaatacaccg ttcaggccaa
     7921 tgacaactgc tgggacattg ccgttgctca gaatgttgag ctctcggtgc tgttagccat
     7981 caatggcttc acccccggca cctgcccgat tactgagggg caagtcatcc tcattccggc
     8041 ccccgaccaa accctgccta ccgccacgcc gctcccgccg gatttgcctg ctggaacgcg
     8101 catcgagtat tacgttggcg caggtgaaac cctgggcgtc attgctgaaa agttcaatac
     8161 caccgtggat gccattgtcg ccatccgaga taacggcctg accctggata ccatcaacac
     8221 aatctatgaa ggtcagaaac tgatcatccc ggtcaatctg gtcacaccga ctcccaccaa
     8281 gcagcccacg ctgaccccgc tgccagccac cgcgacgaca gcgcccactg ccacccccac
     8341 accgtaacct ttctgacgtt cacatctttt ggagggcagc ccgaatctcc atctggactt
     8401 ctggctcgcc ctccattttt tgagcagccc ataagatttg ctggctggtt gtcgaaccaa
     8461 tttgccccag cgcccaggcc gccgcgccac ggattaaggg ttcgggttcg ttcagtaaca
     8521 cattttccag cgccgggata gagcctgtca atcctgcatt gcccagcgca gtcgccacat
     8581 tgcgcaagta cccgcgccgt ttggcgcgta aaataggtga atccttatat ctcaaattga
     8641 attctgtcgc agacagcccc agcgcttcaa tcagtgccgg tgtggctgtt tcggctcggg
     8701 gttccagggc cgcgtcgccg gctgtgttca gccattttcg attccacggg caaacctgct
     8761 ggcaaatatc acacccaaac acccggttcc ccagcgccgg gcgtagttcc ggcggaatgc
     8821 tccccttatg ctcgatggtc agatacgaga tgcatctccg ggcgtccagc gttcggtcgg
     8881 ccccaatgca gccggttggg caggcctcaa tgcagcgcgt acaggtgccg catcggtctg
     8941 cggtaaaagg cgcatcggcc tcaatctcag tgtcaaacag taactcgccc agcaaaataa
     9001 aagaccccca tgcagaagag atcaggcagg tgttcttacc aatccacccc agtccggctt
     9061 gctgcgccag atcgcgttcc agcagggggc ccgtgtcggt atatatcctc caattaaatg
     9121 tcttgcgact ggctttctga aaatcttcgg ccagccattg cagcttttct ggaatcacgc
     9181 tgtgataatc tttgccccag gcgtaggctg ccacttgccc gcgcgtcggg tcgccgggca
     9241 gtaccgccgg ccccggcgcc gcatagcgca gccccaccac ggcaatggag cgtagccccg
     9301 gcagcagttc agcgggattt cgtcgtttta ccactgcgtc gggtcgcgcc agatagccca
     9361 tttcagcctg tttacccgcc tcaatccatt tttcataaac ctcaatatgg gtgggcatct
     9421 gtgccggact gatccccagc aagtgcagcc cgcgcgcttc agcacaatcc tgcaggacct
     9481 gacgcagtcc gttcacggca taaaatactc agccagcaaa ctgctcaggt cagcatcagt
     9541 atgtcgggca gcaatttcaa aaaaagcttc ccggtcagcg gtttgatttt gcagtgtttc
     9601 agcataatct ttcagaaacg caaaaaacgc ctcgtctccc atggcggttc gcaaatcttc
     9661 aaaaaattgt gccccgcgca aatagacggc attccgatag ggcaggtagc cgccgtagtc
     9721 ataaatcccc cggttgatcc agcccgccgg gttataaaaa tctacccggt acgcccacca
     9781 ccagttcacc gcctcgggcc ccgattgttc gtaatataac cgttcgcagt aggtcgccag
     9841 ggattcatcc aaccagggct ccagcccttg atcgttcccc acgcttccaa accaccattg
     9901 atgtgaggtc tcgtgcacgg caatcatcgt caggtaaccg cggtccgtgc cttcatacag
     9961 gctgtaaaat ccccggctga ggaagtacag cccatcatat tccatcccat ccaaaaaatc
    10021 agcttccacc acgctcaacg tagtccgtgt gtagggtgtg aacagttctc cgtacaggct
    10081 gaccgcctcg ctggttgctt ccagtgctgc ctccgccgcc ggatgagaga acgggaacag
    10141 cgaataactc aacaccgtga tattcccgac ctgtttctca aacaccgtat agaccgggct
    10201 tcctgatatg gcaaaactgc ggcagtcttt cattgtgtaa tgataccggc tgccgtcttg
    10261 ctcggcgggc gagctggccg ccagcaccag cccctctggc gcgttgctca catccagcgt
    10321 aatgtcaaaa tccgccagct cgtacacctg atgttcgccg taataccagg ccggatgcgc
    10381 cagccagccg cgcccggctt catagggcgg aaaatacaaa taccagtcca ccagattgct
    10441 ctgattgacc gtatatccaa atggcaccgg gcggtagctt tctgagggcg gcgggatctc
    10501 tggcaggaca atagtatagt gggcctgcag tgcaattcgc tgccctggca tcaggggggt
    10561 atccagcggt atggtcaatt gatttgagtc cagcaccgca ttgggatagt tctgcccgtc
    10621 tgcgccggtt aaggcctcca gttcaaaggc tcccttccag cggttgggct cagccatcaa
    10681 taccagttgg gtgatgggtt ccgtagaatt gttcgtatag ccaatgtctt gctgaaccac
    10741 cacctggcga tacccaaagt cccattggac attcaaggta taggtgattg gctgcggcgc
    10801 tgctggagta accgacggca gtgcggttgg ctctagcgtg ggtgtttcaa taaaatcgac
    10861 agttggcgca gcctcggtcg aggccggcag ggtcggatct gccgggggtg gagtttgtgt
    10921 acacgctccc agcagcaggg cactcaacca gaggatgcgc cagactcgtt tcatttgact
    10981 tatgatcagg gcgtgggcgt cagcgtaggt tcaggcgtat tggttgctgt agattctaat
    11041 gttgccgtgg cagtggacgc cgccccctca accttgatgg taatcgtcac gctgtagaag
    11101 tttgcgccat tggcattggt cagcacccag atgctgtcat atgtgccgcc cgtggtcggg
    11161 gccttcatat tcagtgtgag atccacctct tcttccggct taacttcctt cgggaagctg
    11221 atatctgttg cgctcatccg cccgccgctg cctgcataat accgcagttg gtagccggtg
    11281 gtccacgtcg tggtgccggt attcttgata tgccaggtca tggcaaaatc ggcgcctggc
    11341 gcaaaactgg tattgtcagt tggcgattgt gaaatccaca aaccccggtc actggttccg
    11401 ccagccggcg gcgttaccac tggggcggtc actgccagtg tggcagttgc gccggtggcg
    11461 gttgcgccta ccgtggctgt gggtggctgt ggcgtgttgg tcggcgccag agtccaggtc
    11521 gccgtgggcg agggcatcat tgccgccgtg gcagtcatcc aggcctgcac ggtttgcgct
    11581 ccctgagtgc gaacctcatc cgcgctctgt gtgggttcaa ccttgccgca ggcggccagg
    11641 gtcaaaaggc tcaccagcat cagggcgccg atgagtattt ttttaatcga agtcatatca
    11701 ttctcctaac taaattacaa aatgagcggc ccagaagggc cgaaagggaa ttgtccttcc
    11761 ctttttcgga attgtcaagt gtctaccttg gtcctatggg atcgggatcc agccttcagg
    11821 agcatcctga gaaggcagtg cattgctgaa tccgcgccca tcccggaaca caaaataggt
    11881 cactgcgcca ttatgaattg agaaatcata agtgtaatcg gtgccgcgtt gtgtaaagct
    11941 tagcgtataa tcgccaaccg gcaggtcgct cagcactaga ttctcgcgat aatattcatc
    12001 gctgttcaac cagggcgcgt aggacagtat aatccatttt cgcccggttg tctgagattt
    12061 tacagtgact tctaccccgc tcagaaacga accggtgcca tccagcagcc ttcccaccag
    12121 cacccccatt ccttgcggcg gggtcagcca caattcaggg ttgcggtggg tgtaataccg
    12181 ctcgcccgat tcaagccgca cctcaaaatg caaatgcggg ccggtggtat tgcccgtgtt
    12241 gccaaccagc cccagcgtat cgccggtttc aacccgctgg cctaaaatta cgttaatttc
    12301 atccatgtga gcatagacgg tctgcagctt atgcccttca aagccaaagt catgccggat
    12361 catcacggcc aacccatacg ggtcttcggg atcatcgtcc ccatgcagca ggccgtatcc
    12421 agcccacacc acccgtcccg atgctgccgc aatcacaggc gtccccggag gggcgtcaat
    12481 atcaatgccg gtatgtaaaa tggtgctgtc tggaaataca tagccatacc ggtaatctgc
    12541 cagcggccag ttgacctcat ccacggcaat gggccggctg aaataaaaat gatctttggc
    12601 ccccagcgcc cagggtgctt cacccagggg cggccgccac aggaaagatg ggtctgcccc
    12661 cggtgtagga aagaccaacc gcagcggatt gggatcgtgg gtatattctg gcgttggcgt
    12721 cactgctggt ggaggcgtac cctctggcat ctgttcgcca gcctcagcaa gcggtggggt
    12781 ggcagtcaag tcgccggctg tatcgccgga tttgcagccc gccagactta tcaataaaac
    12841 gatcaacagg gctaaatacc aatagggttt catctttaag ttcatatctc gcgctgacca
    12901 gtctctcatc caaaaaaaag tattgattat ggattcggtg tgggcggcgg cagcgtccag
    12961 gttgggcgca gcgttgccgt gggggtcagg gtgggggtgg cttgtgtagg gcctcgggtc
    13021 ggtcgcggag tggctgtagg ctctggcgta taggttggca cataggtcgg cgtaatcaga
    13081 gcctcaaccg ggtacaactg atccattgcc gcctcccagg tcaatccacc ggtcatcaca
    13141 aactgattaa atcgcgtggc cggataatag gttggccaat tgcggaaggc cgacagccgc
    13201 tgccagccat atcgagccgc caggtcagta aaatctatcc agtaaccggc tggcacaggt
    13261 ccaacctgtc cgcccgcctc atacgccagc gggtcaccgc tgtaacgggc gttcaaatcc
    13321 cagatcattt gctgtaacgg cgtcccctgt gagccgtctt gataccgcgc cttaatatac
    13381 acccgccagt atgtctgact gccgatctct tcccgaacag ccaccagcca gcccgcatcc
    13441 agcggaattg ggttcaacgc aaaagcccgc ccggtgtaca gccagtcttg cgtcaccccg
    13501 ggtgataccg gataggtgat cggtttatag gcgttttcaa ggctgagcag aaaatcccac
    13561 ccggcagtga caccaacgcc agcccgcagc gcactgtaag cctcatccac ctgatcatgc
    13621 aggtacgggt agggtgctgt cacgtccggc agccccacaa tgccgtaacg cccggccggg
    13681 gccatctgag tcagtgaaat ttctggcgtg taaagcgttt ctggtgcggg caggttaatc
    13741 tgcagcagcg gcggcagggg gtcatcagca ggctgtgaca gggctttcca ttccagccca
    13801 cgcacagccc ccggcaggtc caggaagggc aaaatcattt tggccccatc cagcgcgtag
    13861 gccgtcaggc cggtttgatt cgggcccacc accgccgtca aaatagcatc gcttcccggg
    13921 ctccaggccg gccagtcacc gctgcccaat gcccgggccg ggttgcccgg cgccgcagta
    13981 tcccagacca taatttgtcg tgtcccctgt ccatccgccg accaggccag ccaggcgcca
    14041 tccggcgacc aggccgggtg atcttcctgt acgccgggcg tctgactcag gttggtgaac
    14101 cgctcgccct ggctgtccag ccgcgcagtc cagatttcct gattgccgtt ccgtgtggag
    14161 gtgaacgcaa tttcacgccc ctgcggcgac cacgccggag aaaaatccgc cgccgggtca
    14221 ctggtcaatt gaatcgggtc ctgggtcggg tcactgatgg actggatata aatttccaaa
    14281 ttgtcgttgg tataggtttc gtaagccagc cactcgccat ccggtgacca cgttggggct
    14341 ccatcgtagg ccggggtgtc ggtcacccgg gtttgttcgc cggtttccag gtttaacagg
    14401 tagatatccc agtaaccgtt ttggcgtgag gcataggcca aaacctttcc atccgggctg
    14461 accgttgggt cggcgtcatc ccagggtgta ttggtcagcc gcaccagcgg caaatattcg
    14521 gggtgatagg caaaaaggtg cgtgtacagt ccatcggtca gcgccagaaa aacaaagccc
    14581 tggtcacgca atcccgcagc ctgttcatta ctgattgggg taatttctac cactggtgga
    14641 ttttctagca ctgccgcggc tgttggcgag gcctcccccg tcggcgtgac ggatcgggtt
    14701 acggttggac tcaccaccgg ctcgaccggc ggccagataa aagctgatac aatcgccccg
    14761 accagcagca aacccgccag caccatgagg atgatgcgga cacgcttgcg ggtgaaaaat
    14821 gctttgagtt tacccattgc tatgatttta cttcaaatcg tcgcggagca gtagcgccgg
    14881 gtcaatcggt tggaaaatcc cgctgagccg ccacaggcag taatacccca tctcgtccgg
    14941 tgtggcattg ttcgcgtagt gcgccatcac cgggaagttg gcccccgccg gccccacccg
    15001 tacctcaata tgcaggtgtg ggttcacgct cgcccccgtc gtccccacct cgccaatcgt
    15061 ctcaccgcag gtcaccgcct cgcccactgt cgatagggcg ggctgattca aatgagcata
    15121 aagaacatac agtgagcgct ggctgttgtc ccactcgggc gtcaccggca gcgccgggca
    15181 gttcacccgc ccatcgggtg tggcaatcat ccccggctcc ggcagtgcca gcatctccag
    15241 ccagctttca ggcagtcttt ccagcggcgt ctcaacaata atcatgtttc catacggcgg
    15301 gcggtcttga atcgcagcca ccgtctcccc atccagcacg ctcatcaccc ccagccccag
    15361 catctgactg tgctccccgc gtgagtaata cgcataatcc gccccatggt gcccatcatc
    15421 ccgcccgggt gacggcatct caaacggatt tttgagaatc tccggcagct ccgccagggt
    15481 gatatcttcc agcggtgaac acaaccccga aaacacgggc ggctctgggg tttcggttgg
    15541 tgacatcagc gttggtgttt cggttgccgg ttttacgggt gggttgggga gcggtgttgg
    15601 gctttcaatc gccgtggtcg ccgtgaccgt gggcagtgca gaatcaccca ctgtgggggt
    15661 gcagccagcc agcataaaca atgccagcag cagcacccag ccaccgtggg tgattcgatg
    15721 attcagtcgt aacagcttat ccgttcgcag cgggcgtttc aaagttcacc tggcgctcgt
    15781 ccacgcattc caggtcgggg tagtaaagcc gcacggcggc tgtaaaaggt cccaccgcct
    15841 cggccgaacg gatatgcatc gtgaatttta actgttccac tgccgtttca atgatatgcg
    15901 tcactgaaac aacctcccca ttggccgcgc tgattcgtat ctccagatta ggcggctgct
    15961 ggaacggtgt gatatccacc aggatgcgta cccggcgtcc gtccggatat ggctctaccg
    16021 ccaggtcaac cagccgcaca tccgcaggcg ccagggcgtc ctcatccggg ggttggaggg
    16081 caaaatgtgg cattggggca gtcacctaag cggtgatttt ccgggcttcc atatagaagc
    16141 gcttctcttt ggcttcaccc atcgagaagg tcttgcgcgg cagcgcgcca tccagaatca
    16201 cggtcttgaa caggtcgctc tttggcattc cgggcaggta aaagcccagc ttgcccggct
    16261 gcgcgcccag ttcgcaaacc acatcttcgc cgtgcacata atcaatcttc tcggcgccgc
    16321 cgtttttcag ccagccgtct aaaaagacct gcagtgtgcc aacgggcagg ttggatgtcg
    16381 gcttggtgat ccaggcaacc tggtatcccg cgctcgagat cacgccgacc gcctgctcaa
    16441 cgccttgctg cccatccaca acctcaacca tttcagccgc gctcttgcag ctctggaagt
    16501 gaatggcccc accgtaaaaa gcggtcattg cctcaaccac attctgcttc acgccgaaca
    16561 acacccgatg aatcggttca aattccaacc cctcatcatg gacattctca atctccacca
    16621 gggcatagcg cgccgggtga ttcatgccaa cggtgggctt caatttttcc cagatggctt
    16681 tcgccgtcgc cagcgagtga ttgccgtcgc ccatggcaaa cagcagcacg cccttgtcgg
    16741 ctcccacgcc gtagcgctgg gcaaatttct gcgggtcagc cagcttttcc agcgcgccaa
    16801 ccacctgctt ctcaatagcc aggtccgtca cccggtaccc gcgcaggtga ccactgccca
    16861 gcatcagttc aaaatcgtaa accggcacaa ggtggtcgcg ctgtgctgcc atcggctcga
    16921 tgaccgtttt ctccgggtcg tcaatcagca ccagaatatg cggcagttcc agagccgcac
    16981 cctggcgaat ccgcacccgc ggcgggatcc gttccaaaat cgtgccctca gtcgcccgga
    17041 taagcgtttg cgagcctttg ttgtaatcat atcgttccag gtccagcgcc agcatcaaac
    17101 cgcggcgtgt ccgcccttca acggttcgct caacgtaaat cagcccttca taagccgtca
    17161 gcagatcttt cgccaggtag gcctgcatcg cctgctgtgt gctttcaatc cgccggggtt
    17221 cttctggcgt ttccagataa acttccggca gcgtcagccg aaatgttgat ggcgcactgc
    17281 cgaccatatc ttcgacctgc tgccagtatt ctggctgcga ggtgaactgg tcgcaggcaa
    17341 tcacagccca tttggtcagg tctgtgccgg gttttggcag cagaatatca gcaatctgga
    17401 tgccaatttc agggtaatta cgcatggatt gagcctccca ataagtgata tgcttacagc
    17461 gtattttacc ctaaatcaaa accttaaggc gtcttatcca gcagcgcaat catttctgca
    17521 accatgttgg cctgccgcgc ccggctgtcg gggtcaaggt ccatttcggt caggcaggcg
    17581 gccgtataat cttgcagaaa agtccggctt gtggcctgca ccgccgcgtg aattgccgtc
    17641 agttgctgca taatctcccg gcagtctcgc tcttcattta gcattgcctg taccccgcgc
    17701 acctggcctt caattcgccg cagtcgctga actaaccggg tcttggtttc tgtatcttga
    17761 attttcattg gaatattcct tgcgttttac cggctcacgg ccggtcaggg cttagcccgc
    17821 tcagccaaac ccgccgtgcg agccacagct gctcccgccg ctgctggccc cgcttgtccc
    17881 gccgccaaaa gcagccgcca ttgagacttt cttcttcgta tccgtactgg cgcacttggg
    17941 gcataccggg ctcaaatcca cctcggaaaa ccgcaccatc ttctcaaacg tctcgccgca
    18001 ttgattgcac cgatattcat acaggggcat ctaaactcac ctctccaata gttatatact
    18061 cccccctagt atagcatatc gcaaaccctc gtcaaggttt tgcgccagct actcgcagat
    18121 tggttgtata aattcatccg cggcccagat tttgtaggcc gcacgcgcca atttctcgtg
    18181 ggtcgtatgc cagcgtggca ccgcaccgat atcccaatac tgcagcccca gcccttcatg
    18241 cgagagagcc aattgaccac caatgacatc gcacaaatgc acaattgaaa gcgtggtgtg
    18301 ggggccatat tgggcgctcg gttttcgcgt gaacacgccc accagccggt taatttgaac
    18361 ttccagcccg gtctcctcgc gcacctctcg aataatcccc tcaacggggg tttcattggg
    18421 ctcaacaaac ccgcacggca ggcaccagcc gctgccatcc gctcggtcca tcaataaaat
    18481 ctctccccgg ctattaaaaa tagccgcgtc agtacccacc ttgggcgtga tggttccaat
    18541 ttcgcccaga aaccgctttt tgatctctga agttggcgcc tctaatgctt cgctgtacgt
    18601 ttcgcaggcc aggtccatca atcgctcata ccgctcccgg tcaaacgggt tggcggcata
    18661 gtgcagtccg tctcgcgcaa tcgcttgcaa tctgtctaaa agcgtaaaaa tatccatgct
    18721 tatcctgttg aaaaataaac caggtgtgcc ctggagaaca cacctggctc tgtttatcgg
    18781 tattggccca gcgccattaa tacgacgaat tggagtgcga aatctccagc ccgttcaccc
    18841 gcatgaatgc cggcacaatc attgccgtgg ggttgcgcgc cccatagctg cttgattggt
    18901 tgctgaccac ctgggttttg gatatccagg ccactttgtc gccaaaaata tccagcaggc
    18961 tttgcgtgac gcgcacattg taaggaacga caatcgagcc gtctttcttc agcagcagcg
    19021 ccccaaaccg ggaactgccc gtgaacaggc cccgtgacgg gttgaccagg ttcatgtaat
    19081 gcaggaaggg gatgtacagg atatccttgt cgcgcggcat ggctgccagc tctgccagtg
    19141 tactgacatc catcgttccg ccgttgatca ccagactctt gtgcatcacc gtatgcccgg
    19201 tcggttgcgc gccaaattca tcggcatcgt cctgatacca ggtaaagccc ttgaagacgc
    19261 cattcaccac cagagggaaa gacttgcgcg gcatgcccat aatatcccgc cggaaggggt
    19321 aggtttctcg ccgggtgggg tcatccgtca ggctgaactg ttctgaaaat actttggttc
    19381 caatctgggc ttcactcagg aacgagtaac cccgtttcat cgtgccgcca ttgaagccaa
    19441 cgtagttcat aaagttcatc aaatccgcaa ttgctgccga gccaaacaca atatcgtagc
    19501 ttcccacggg cagctgctgc ggcgcatcat gctgataccg atccagcagg aagctcagat
    19561 cgctgcgaac agatttcgga ttcaggtcac ttttttgttg agccgagcgc tcggcaatca
    19621 cttcccactt tagcttggtg tgtgacagca ccacgctgat ctgtgcatct gaggtcacaa
    19681 agtactgggc gtgctctgat cgggtattga tctgcgcagt aacattcgtt ccgtttgaga
    19741 aaataccgcc cagcttgata tcttccgttt ccaatccatc acaggctgca ttgaagtacg
    19801 ccagacgcgc ttcgttgctc agctttgcca gctccgcatc gtaggcgctt tcatcaatga
    19861 aagtctcagt gtactgcggg atggtcggct cgtagttcag cggttgggca tgtttcacca
    19921 tttcagccgc aatgtccacc ccttgcttca tctcttccag cttgcccagg tgcgtaatca
    19981 gttcgtaact ggcacgcttc cgaccctcat aagccgtaat ctccagccgg ataagatgtt
    20041 cattggtatt cagcgagatc gccgaattgg caaaccgcat caagtagctg tcttcttcgt
    20101 gatagaagaa cgtggcggtg acgtttttcg agatcgcata ggcgcgcagt tcgcgcaggg
    20161 tttgcagaat tttctccttc acggttactc tccaattctc acattgtcaa atcggcagac
    20221 cggcacgcca tgccccagct gcataatttg attgggttga cccttgccgc agttatccac
    20281 ataacgcact tcccaggtgg attgatcgcc caccgcagac agcgaattgt agaatttgag
    20341 cgtctcaccc cggtaggtag agttcttcac cacccgggtc accttgccgt catccaccag
    20401 ccagccaatc tcggttgcaa agtggaactg ctcgcggttc gagccgatgg accagctctt
    20461 ttcaccatcc aggatgatcc cgcgctcggt gttgcggacg atatcatcca gcgtgccgtc
    20521 ttcgcccgga tcaatattga tgttggtcat gcgctcaatc ggcacccggt agaaggctgt
    20581 cgcccggttg gcgccgctgc tgccatcaaa aatctgcttc cgtgctttgg cattggcttc
    20641 ttccaccatc tgccgcccgg tgattgcgcc caccagaata cccttgtcaa tcaataaatt
    20701 attctgggcg ggcacgccat catcatcgta gccaaagctg cccggactgt tggtcagggt
    20761 ggcatcggcg cgggcggtca gcttgggcga accgtagcgc agcttgccaa agtcgtccag
    20821 cgttacaaac gagccgcccg caaacgccag ctcgtagccc agaatccgat ccagctccag
    20881 cgcgtgacca atggtctcat gcgtctgcag gaacatgatc cccggcagta aaatcaccga
    20941 gcggtcttcc ttggggcagc tttctgccgc cagcacctcg ttcagttccc gcacaattcg
    21001 gtctgcgttc tcactgaaca tctgcatatc cacggtttcc cagccgcggg tggtgcccat
    21061 tcggcttggg ctgaacttgc gcacctgcga tacgccgtct gggtccacgc ccatcacctg
    21121 gatttctggg aagacctcaa tgatattctt ctcaatttcg ctgccttcag agtcaaagaa
    21181 aacgatctgt ttacgcacaa aggtcagcgc gcagatccgc tgcatcacgc ccgggcggtt
    21241 caacttctca tccatgacct gcataaacgc aattttatcg gtcagcggca ccccaaacgg
    21301 atcaatacgg ttggggctgt tgaaactggc ctgaatggca tccttctctg ccagccgcac
    21361 cggaaaactg acccgctggg cggcaattcg ggcattgctg agtgcctgat caaacagcgc
    21421 gctcaagttg ctcaaatctg aagaagccga aaaaccccat gccccgcggt acagcacccg
    21481 aatgcccagc ccgctctgcc gcgatgccgc agcattcttc aagttgccgt tccacatcac
    21541 cagttcgctg gtttcctcaa ccgggtacca gcgcacatcg gcatacgtcg cgccagcttg
    21601 cttcaatcgt tcaatttgcg cttgtaactg tttctgcatc agcccctcct ttgggggttt
    21661 cattgggttg aatggtggat aaaaatgggg attgcgcttg cgcgccagat atttaacaag
    21721 atcccaattt cactgccttc ttgggattgg aaacgaataa attatagcat catttcaatt
    21781 cgggctgacg caaaaatacc gcccggatgc gcacctatcc aaaatccacc cgcggccgat
    21841 actgcagtgc ctctgccaga tggactggca gaatcacctc actgctggcc aggtctgcaa
    21901 tcgtcctcgc cagcttgaga atccggtgat acgcccgcgc cgataattcc agttgctgca
    21961 tggccgattt catcaggctg cgcccggtat catccagcgc acagaactgc cgcacttccg
    22021 ccggtcgcat ctcggtattg ctgctgagcc cggctgtcgc caaccgttcg cgttgaattt
    22081 gccgcgccgc ctccactctt gcccgaatgg tcgccgaggc ctcacccatc cgcgcatcgc
    22141 tcagcttttc gtaatccacc tgcggcgttt ggatgtgaat atcaatccga tccattagcg
    22201 ggccggaaat ccgcttctga tacttagtca ccgttgccga ggagcaggtg caggccttca
    22261 tcgggttccc aaaattgccg catgggca
//
LOCUS       DAOPVS010000022        21695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2409_length_21695_cov_57.701617, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000022 DAOPVS010000000
VERSION     DAOPVS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21695
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2409_length_21695_cov_57.701617"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKL11_03700"
     CDS             <1..401
                     /locus_tag="PKL11_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ12737.1"
                     /translation="DNEAYVIEALRNGAYGYVLKDSTADELIQAVRIVAEGRRFLSSP
                     FSERAIAAYSQKAQSAPLDPYETLTTREREVLHLAAEGLSATDISARLSISPRTVEIH
                     RANAMHKLNLRSQAELIRYALRRGILPMED"
     gene            complement(408..890)
                     /locus_tag="PKL11_03705"
     CDS             complement(408..890)
                     /locus_tag="PKL11_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNZ12738.1"
                     /translation="MDEAQLFVDTVITARAAWQAAVNQIPLEDWEQAGFCGDWSLKDV
                     IAHIAWYEREMIHLLETRQFEGSELWELPTDERNAQIYLLKKDQPLTQVLASAIMTYK
                     QLLPLLEQISAAELLDPAAFAGMPPEWTPRQILAGSTHLHYDEHRQQAEAWAKNHPQN
                     "
     gene            complement(934..1674)
                     /locus_tag="PKL11_03710"
     CDS             complement(934..1674)
                     /locus_tag="PKL11_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNZ12739.1"
                     /translation="MDIHNTLSKLDQLGCAYAFYYHPTGREAVFEANCERFSSASIIK
                     IPILLSWIHLERQGLVDRAELCDLDSEPQVQGAGFSWLLRGRKLPYQDVLMLMIALSD
                     NLCTNLIIERIGLARLQQTMRETLGLTGTQIQRKLMDFAARERGLDNWVAASEAIRFY
                     ALMSALTPTERDWAEAMLLVNQDDALLKRNLTRDTVDFYHKTGSVAGVLHDWGYTRST
                     EMFLFTQKVPQEQPVFEVFGELGELLAG"
     gene            1862..2851
                     /locus_tag="PKL11_03715"
     CDS             1862..2851
                     /locus_tag="PKL11_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12740.1"
                     /translation="MAELLQVQGLETQFRTREGIVHAVNGVSFNLKEGETLGIVGESG
                     CGKSVTVMSMLRLIPNPPGRVVAGTAMYQGKDLLKVSNEEMRHIRGAQISMIFQDPMT
                     SFNPVLTIGRQVSEPLETHLGMPRKQAYDRVAEMLSLVGIPNAKDRLNDYPHQFSGGM
                     RQRVMIAMSLTCSPQILIADEPTTALDVTIQAQIVDLVKQLRDQLGMSMIWITHDLGI
                     VAGLAERVNVMYAGYIIETAPVKTLYGDPRHPYTLGLLGSLPRVDETDRRRLVSIEGL
                     PPVLYEKPQVCPFAPRCSHRVERCLKENPPLTQVGPEHFAACWVDTTTGREWQ"
     gene            2842..3825
                     /locus_tag="PKL11_03720"
     CDS             2842..3825
                     /locus_tag="PKL11_03720"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNZ12741.1"
                     /translation="MAMSQEILLKVENLVKYFPIMKGAFIRKQVGAVHAVDGVSFEIY
                     KGETLGLVGESGCGKSTTGRAILQLHRPTSGSVIFEGRDLVKTKGEELRLMRRKMQMI
                     FQDPYASLNPRMTVGEIIGEPVLVHGIMDRKASEKRVEELLSLVGLNPAYANRYPHEF
                     SGGQRQRVGVARALALNPSLIVCDEPISALDVSIQAQVVNLLEDLQKQLGLTYLFIAH
                     DLSMVRHISNRVAVMYLGSMVELATRDDVYYHPLHPYTQALLSAVPIPDPIAEEKRQR
                     QILKGEIPSPINPPSGCRFRTRCPHATDICAEKRPEWREVTPGHFVACHLV"
     gene            3940..5712
                     /locus_tag="PKL11_03725"
     CDS             3940..5712
                     /locus_tag="PKL11_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ12742.1"
                     /translation="MRSKLFTLIGLLVLASMVLAACTPPAAQTPIVETVIVEVAGTPQ
                     VIVVTPEPVVETKPAVLRINMGTYPDIIDPQKSSFVNEIAHLKLFYEGLTRLDQALET
                     VPGAAESWEYNEDATQLTFQLRPDLKYSDGTLLNAKRFEYSILRNIDPATAGEYAGIT
                     DEIVGAPEWRMAAAEDLEANGDALKAAVEVRALDTAGNACVDYEQVDCTVLQLKFSKP
                     APYFHTVMSLWVTFPAKEENISEGGDIWWNSSKFQIGNGPFIMKTLEPFVRAYFVPNA
                     NYWAGTASYDVEYSFITEGAVAFEAYKNNEFDIIGLGADDLATVQADPVLSTEANIYP
                     GSCTYAFYMNKDKPPFDDPKVREAFSYGTNRDAWVTDILKGLGAPTLTWIPKGFPGYD
                     ETEDRWQYDVEKAKATIAESTYGSFEALPEITLTFSGTSRNIPRFEWLANQWTTNLGV
                     KVKLDPVESTTYTALTKERETLPQVFYLGWCADYADPQNWLSVYWMTTSAFAQRYGFS
                     NPEMDALMQEADATLDPVKRADLYLQAQKLLVGDAAVIMMVNNVNSYLIKPWVKDVKF
                     TPMDAGWPGDVDPLSITIDTSLIP"
     gene            5891..6883
                     /locus_tag="PKL11_03730"
     CDS             5891..6883
                     /locus_tag="PKL11_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12743.1"
                     /translation="MVPYILRRLLTMIPVLIIVSGITFILMHAAPGGPWDRDPGARQV
                     DANTQKLLTEYYGLDKDPFFQWTTYTFFGENKDGKVVCGAICGNLGPSYRQRGMTIQQ
                     ILFAPPEGRPWYYSRFGYSLRLAFLAMSVAILIGMPMGILSALKQNTGWDYVAMFIST
                     LGVSIPSFVVAIFLIILFNELDLITIVPKSWDSLGVWVLPAFVLGFGTLSRTARLTRA
                     SMLEVIRQDYIRTARSKGLAEGTVITRHMIKNALIPVVTILGPNLAGLITGSFIIETM
                     FSFPGMGRAYVTAIGQRDYSMIMGTTLVFAILISLANISVDIVYGFIDPRIRID"
     gene            6893..7876
                     /locus_tag="PKL11_03735"
     CDS             6893..7876
                     /locus_tag="PKL11_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12744.1"
                     /translation="MAETPSKIVTFELPEKRRSLWGDAWYRLVRNKASVAGMIIILAF
                     FLIAIFAGAISKQNPLEVHSGTDYLPPVWVEKGINGKPSSPDYLLGTDTLGRDVLARV
                     LYGARVSMVVGIVPTLIVLIVGTIVGLIAGFFGGIWDNLLMRFTDIVWSFPDLLFFII
                     IMIALRDTFIGNLLNGLFLLFAALAMVGWVGEARVVRGQVLSLKEKEFVEAARCIGAK
                     NGRIMFKHIMPNALGPIIIMGAMTVPGMIITEATLGFLGLGMRPASDPNNLPFFVTSW
                     GVLMNEGNAAIHAQPWLLLMPAICVSLVVLAFTFLGDGLRDALDPRMQGTS"
     gene            7992..9953
                     /locus_tag="PKL11_03740"
     CDS             7992..9953
                     /locus_tag="PKL11_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12745.1"
                     /translation="MRRKIVSLLALFLAASLLLTACRTTEIPVAPADLTPSPAAATAT
                     PAPTPTPTPTPRVLTICMGQEPSTLYIYGGSSRAMWSVLEAIYDGPVDTRNYTAQPVI
                     LQKLPTLTDGDARYTPVDVAFGAPIVAADGSLSNLVAGVRYLPAGCTADACAVTWDGQ
                     SPVQMDALTLTYHIVPGINWSDGAPLTAADSVYSYTVAADPLTPINKRLVSRTASYLA
                     LQDDPLATEWVSIPGYRTTNFTEAFWQPLPQHIDSLYSADALLSAEEVARYPLGWGPY
                     IIKEWVTGGHITLERNPNYFRAAEGLPHYDLLVFRFLGETADGNLAALRNGECDFIDQ
                     TTSLLDGFQQALEYEQAASIKLWLGLGPEWEHLDFGIRPATLDDGYNAFSDERPDIFG
                     DVRVRRAFAACLDREGLIRTFLYSRSAVPTGLFPPNHPLYAASLSPAAYDVAAGSALL
                     DQVGWRDYDNAPATPRQAYGVPGVPDGTPLTVTYLTASALLPNAAAEYLAASLAQCGI
                     GVTITALTPGQMYAAGPDGPLFGRKFDLAQFSWASGAETPCYLYQTDQIPTAQNGWVG
                     ANLTGYSSPAYDAACQAARLSAGTDAAAAANLEVQRLLSEDLPVVPLYYRIKLTAARR
                     DLCNYTFDVSARSELWNLEAWDAGPLCAP"
     gene            10291..12873
                     /locus_tag="PKL11_03745"
     CDS             10291..12873
                     /locus_tag="PKL11_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12746.1"
                     /translation="MKRNTMFLVLSLIIVASMALAACTPTVAPTDAPVVTEAPVVTEA
                     PVVTEAPTAIPTPEPTTRHGGWFDEIVISVVDNAAAVTQIEAGAIDIYADGVSSDTLP
                     EIEAAGLGYSSANGLYYELTFNPVGPVFESTGKVNPFHFAAAREAMNWLIDRDYLNRE
                     IYSGGALAKFFPLVTNMPDYTSIAPTIRRLEAEYAYNPERAKAAMDEVMLGIEGVTIE
                     DGKYMFNGEQVTLILLIRNDSDKTRVPIGDYVGNQLESIGFATDRQYKTSSEASPIWV
                     GGNPADGLFHIYTGAWSATAISRNEADNFQFFDSPSSAYGFTALWQANNIDEEYKELA
                     DDLAYTNFNTPEERLEALERAVELSLKNSMHVWLIDGKRFIPYKDGLTVTYDLAAGVA
                     GAQIWPYTLRQEDQEGGQLKWGSPDLFVDPINPIAGSNWAFDQPIVRSTYDQSYMYDP
                     YTGLIWPLRFDKAEVTALEGLPIGATHDWVTLDFAPEINVPEDTIVDWDAVNQQFITA
                     GEKFPEGTTAKIKSIVYYPEALFTDVKNQDGTTMDIADFLYSWIISLDIGKEGSLIYD
                     ESMAPNVEASLETYKGFRILSEDPLIIEAYSDNWQIDAEMNVTTLWPTFSYGYAPWHT
                     LAAASLADAAGEVAFSADKADANTTETKTVEWLSLVGGPSLDIMTNYLDQAIADQHIP
                     YANVLSAYITPDEAVARYEAAKGFYEEYGHYMIGNGPYILSEIYLTEKVATLISNPDY
                     VDLADRWSGFAAPRIADVEVDGEASVVLGTEFTFDVMVNFDGEPYASEDVKEVIGLLY
                     DSTGQIVEVLKGELVEEGLYRITVPAELSATMAAGSSKLEAVVVPYVVAIPTFYAFEF
                     VTVK"
     gene            12969..14105
                     /locus_tag="PKL11_03750"
     CDS             12969..14105
                     /locus_tag="PKL11_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12747.1"
                     /translation="MGSVAETTTPTIEKKKSKGESTLVRVLKYSGFRLFSLFVTVVIG
                     IYLTILIANMGGYVDKMMVSEIQERVTMQISMDPKNKTMDPEVKNNLIQERIQAEMAR
                     LDLDQPAAIRSIRFLKNALTLNLGRAMFMTSDLGSRNVKNIILERIPYTLLLMGTANL
                     ILFFVTLFLALALSRKYGSFWDKFVITLSPTSSVPPWFYGIFLILIFAAILKWLPFGG
                     VIDAPVPKDLMGRILSILKHLILPATSLIVSSFFLSIYNWRTFFLIYSSEDYVDTAKA
                     KGLSARDIERRYILRPTLPTIITSFALMLIGMWTGAIITETVFTWPGLGRVTYKAIGL
                     YDTPVIVGTTILYAYLLAITVFLLDFVYALVDPRVKIGGGGGAK"
     gene            14102..15445
                     /locus_tag="PKL11_03755"
     CDS             14102..15445
                     /locus_tag="PKL11_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12748.1"
                     /translation="MKNFLNTLREIGKYPTAIFGVIVVLALIIVAVVVIIKIPYDQAI
                     STWRGGESIVYKNPRNVPPKWYNWFRAEKLVESFDMKEGDEGVSSVTTVTEGGTTITT
                     FTFEFDHSYTSFPQDMVLYFNSEYETQQPFVAIKLFTPDEREIKIASFALTKSHTYPM
                     LQDQKLQKRLNNVVPNIGLFLDPDVEEQIPVQGTYRLEIQATIFESNSVVHPEFVLHG
                     QVFGWAGTDHMRRDLSLPLLWGIPIALSFGLLAALGTSLLTMIIAAFGTWYGGFWDAL
                     IQRITEVNMVLPFLSILIMVGTFYSRSIWTILGATIALSIFTGGIKSYRAMFTQVKES
                     TYIEAARSYGAGDGRIIFKYLIPRMIPLLLPGLISAVPSFVFLEASLALLGIGDPVLP
                     TWGKMINEAYGNAALYRGWYYWILEPAVLLMITGLGFASLGFALDRIFNPRLRGV"
     gene            15451..16407
                     /locus_tag="PKL11_03760"
     CDS             15451..16407
                     /locus_tag="PKL11_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12749.1"
                     /translation="MTDNLLLRVENLKLYFKASKGLVQAVDGVNLNLNTNEAVVIIGE
                     SGCGKSSMAKALLRLLPRNVGTYSGSVYMDGVDVMALDDEKYRRDVRWVKMSMVPQAA
                     MNSLNPVLKVGDQVAEPLMVHHGVSKEAALEQSRAMFQRVGVPVDFVNRYAFELSGGM
                     RQRVAIAMSLVTMPDLVVLDEPTSALDVLTQANIMNLLKRIKKEINTSFILITHDIAT
                     SSELADRVAVMYAGQIVETSDSYRFFTAPLHPYSAKLMASVPKLRSTAEPDFITGQPP
                     SLINPPTGCRFAARCPKRFEKCGEEPPTVTVDGRLVKCWLYQ"
     gene            16423..17466
                     /locus_tag="PKL11_03765"
     CDS             16423..17466
                     /locus_tag="PKL11_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12750.1"
                     /translation="MAKQKHTLEELKSTVASHTGPLLSIRDLHVWFELRRFGFGRAGF
                     VRAVDGVSFNLDRGEAVAVVGESGCGKSSLMKTILGINIPTKGDVYYADVPMGEFSRS
                     QLKWYRSQVGYVQQDPYGALPPFMNIQTILEEPLIINGVKSKQERLERIHKALEEVKM
                     TPVDEFLPKFPHMISGGQQQRVVIARAMINNPTLLVADEPVSMLDASVRVEILKLLRG
                     LQESRNLAVIYITHDLSTVRYFSEYIFVMYAGEVIEKARVADLINNPGHPYTHALLAA
                     TSDPDAKNVDEFQEVPPGEPPSLVKPPEGCRFHPRCKHLIDGLCNKERPQEFEAAPGH
                     FVRCWLFQENGKS"
     gene            17453..17662
                     /locus_tag="PKL11_03770"
     CDS             17453..17662
                     /locus_tag="PKL11_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12751.1"
                     /translation="MERVNPKYWIWIGLVLLLIGVVLPLMMVVRVIEATFFLVFFSHA
                     ASVVGAFMGFYGIAMGYISRKNRHE"
     gene            17836..18993
                     /locus_tag="PKL11_03775"
     CDS             17836..18993
                     /locus_tag="PKL11_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12752.1"
                     /translation="MTQRVMIVDDAMELGRLLKAALEVLGAKLTISVHPSAEEAILDA
                     GKHTVDLVVTDMRLPGMTGVDLIKRMRKRQPDLKVILISGLTDRKIRQQAEELGCEAF
                     LPKPLEMEEFIRVSAAALGVNPPALVGSDPVAGEETPAPAPRREKDKEKPAEPPAADA
                     LAAETQKQLLENLCKSAAALGVVLLDEKGNRLVRSGDFPKIEVAGEWIPPLMTVMQSS
                     NQFANMFADTHPSVGVTMRGAAFDLVMAPVNSGVVMVALKPGSSSIRLALALEEVLAV
                     QPRLAATLAASPAEATDSAPAKSGSKTTAKPAPAPEPEPPAVPEDPAALAAFASLFGE
                     GAAPAAPAVSDTDSFWEQAADQQLAKVNPDTISFDQAVQLGLAPDEPDKEK"
     gene            19030..21222
                     /locus_tag="PKL11_03780"
     CDS             19030..21222
                     /locus_tag="PKL11_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12753.1"
                     /translation="MSNHLAVPFMPGSAPVASLPLGRYLPLLPVGMAAAWARQHLQPG
                     DWVFDPLGAHPLLALEAARAGCRVLVASNNPILTLMLQVLAAAPQTTDLQSALSDLAA
                     LRKGEERLELHFQSLYQTPCPGCGELVPAQAFLWRDGEDQPYARLIKCPRCSAEGEFP
                     ITPSDLERLTPPGAAALHRSRAVQRAALPDEPLGEAVEAAIKTYLPRPLYILSTLINK
                     VEGLLIPAERRRLLYALLISALDEGSALWPWPPGRARPRQINIPPVYRENNLWLALEN
                     AVSAWVNARTPLPVVYWPELPPETGGICIYRGRGRELFPLPPTIHLSAVLTVLPRIQQ
                     ALWTLSALWTGWLWGSDALVPLRGALERRRYDWSGYAGALAQTFAALRPHAPADLPLF
                     AIVPELNPPFSAAVLTALQSAGFILTGAALSPEGDLAQFTWQPAEAGASPGTVLEGMQ
                     VHLRERGEPADFATTYLAGLLPACAPAAPADTPPLQSVQTALENAFHNSAVFYHYKTS
                     ENAALDTGLWGLADSAGSDLPLADRMERFVVTWLQKTPEFMQSALEADLWAEFPGLLT
                     PPQDLLRACLESYAEPTGAPGAWRLRPQEAAAERRAHLKVVYQLLTRLAEQLGYPCSG
                     ESPLIWRGAYAFFPMASAIISRFVSAPQPLPPERCVLVFPGGRASLINVKLRRDPRLA
                     QAARGWHLLKFRHLRDLSGRANLTPAAFADLLDADPPDWEDARQMKMF"
     gene            21361..>21695
                     /locus_tag="PKL11_03785"
     CDS             21361..>21695
                     /locus_tag="PKL11_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12754.1"
                     /translation="MAHFRGIVLRLLVAVVLVLGFSPVHPVQAYIPISGTVTWTIDQI
                     IATDVVVLNGALLTIQSGVSVTMQCGIDPAPYSSGLSTKIEIIVENGGTLNMNGAIIH
                     GDGNGGCWQG"
BASE COUNT         4401 a         6854 c         5630 g         4810 t
ORIGIN      
        1 acgataacga agcctatgtg attgaggccc tgcgcaacgg cgcctatggc tacgtgctca
       61 aagattccac cgccgatgaa ttgattcagg ccgtgcggat cgttgccgaa gggcggcgct
      121 tcctcagctc gccgttttca gagcgtgcca ttgccgccta cagccagaag gcacagagcg
      181 cccccctgga cccctacgaa accctcacca cccgcgagcg cgaggtgctg cacctggcag
      241 ccgagggcct cagcgccacc gacatttcag cccgcctgtc catcagcccg cgcacggtgg
      301 aaattcaccg cgccaatgcc atgcacaagc tcaacctgcg ttcccaggcc gagctgatcc
      361 gctacgccct ccgccgcggc atcctgccga tggaggattg actcccacta attctggggg
      421 tgattcttcg cccaggcctc agcctgctgg cggtgctcat cgtagtgcag gtgggtgctg
      481 cccgccagaa tctgccgtgg cgtccattcc gggggcattc cagcaaacgc cgccggatcc
      541 agcagttcgg ccgcgctgat ctgctccagc aggggcagca gctgcttata cgtcataatg
      601 gcgctggcca gcacctgcgt cagcggttgg tctttcttaa gcaggtaaat ctgcgcattg
      661 cgctcatcag tgggcagttc ccacaattcc gagccttcaa actgccgggt ctccagcaga
      721 tggatcatct cacgctcata ccatgcaatg tgcgcaatca catccttcaa ggaccaatcc
      781 ccgcagaagc ctgcctgctc ccaatcctca agcgggatct gattcaccgc cgcctgccag
      841 gccgcccggg ctgtaatgac cgtatctaca aatagttgtg cttcatccat aaatcaacac
      901 ctcttttatc agcttaaaat ctagcctcgg cgcttacccc gccagcagct cgcccagttc
      961 gccaaacacc tcaaacacgg gctgctcttg cggcaccttt tgcgtgaaca ggaacatctc
     1021 ggttgagcgg gtataacccc aatcgtgcag cacccccgca actgagccgg tcttgtgata
     1081 aaaatccacc gtgtcgcgcg tcaggttgcg cttcagcagc gcatcatcct ggttgaccag
     1141 cagcatcgct tctgcccagt ctcgctcggt aggggtcagg gcgctcatca gcgcataaaa
     1201 gcggatcgct tctgaggccg ccacccagtt atccagcccg cgctcccggg cggcaaaatc
     1261 catcagtttg cgctgtatct gcgtgccggt cagccccagc gtctcccgca tggtctgctg
     1321 caggcgcgcc agcccaatgc gctcgatgat caggttggtg cacaggttgt ccgagagggc
     1381 aatcatcagc atcagcacat cctggtaggg cagcttgcgc ccgcgcagca gccaggagaa
     1441 cccggcgccc tgcacctgcg gctcgctgtc caggtcgcac agttcggcgc ggtccaccaa
     1501 cccctgccgc tccaggtgaa tccagctcag caggatggga attttgataa tcgaggccga
     1561 agagaagcgc tcgcagttgg cttcaaaaac agcctcgcgg ccggtggggt gataatagaa
     1621 tgcataggca caccccaatt ggtccagttt ggagagggta ttgtggatat ccataccccg
     1681 attataattt tcttctctaa aattgggtga ttaacgtaac ctctaatctg gtttgacagg
     1741 ccaaaacttt aaggatatac tcatgaaatt cgtatataat cagtgagtgc atttagcaag
     1801 tctgatttct tcccccaaat atctctcatt acaatccatc taactgaaga ggcaaagctg
     1861 catggccgaa ttattacaag tgcaaggcct ggagacacag ttccgtaccc gtgaaggaat
     1921 tgtgcatgcg gtcaatggcg tttcattcaa cctcaaagaa ggtgaaacgc tgggtatcgt
     1981 tggcgaaagt ggctgcggta aaagtgttac cgtcatgtcc atgttgcgcc tcatccctaa
     2041 ccccccgggg cgggttgtgg ctggtacagc catgtaccag ggcaaagacc tgttgaaggt
     2101 ttccaatgaa gaaatgcgtc acattcgcgg ggcgcaaatc tccatgatct tccaggaccc
     2161 catgacctcg ttcaacccgg tgttgaccat cggtcggcag gtttctgagc ccctggaaac
     2221 acacctgggc atgccccgca aacaggccta cgaccgtgtg gctgagatgc tgtcgctggt
     2281 gggcatcccc aatgccaaag accgcctcaa cgactacccg caccagttct ccggtggtat
     2341 gcgccagcgc gtgatgattg ccatgtcgct gacctgcagc ccccagatcc tgatcgccga
     2401 cgaacccacc accgccctgg atgtgaccat ccaggcgcag attgtggacc tcgtcaagca
     2461 gctgcgcgat caactgggca tgtccatgat ctggattacc cacgacctcg gcatcgtggc
     2521 gggcctggct gagcgtgtca atgtcatgta cgccggctac atcatcgaaa cggcacccgt
     2581 caaaacactc tatggcgacc cccgtcaccc ctataccctg ggcctgctgg gcagtctgcc
     2641 gcgcgtagat gaaaccgacc gccgccggtt ggtttctatt gaaggtctgc cgccggtgtt
     2701 atatgaaaaa ccccaggttt gcccctttgc gccgcgctgc agtcatcgcg ttgagcgctg
     2761 cctcaaggaa aacccgcccc tgacccaggt gggccctgaa cacttcgcgg cctgttgggt
     2821 ggacaccacc accgggagag aatggcaatg agccaggaaa ttctgttaaa ggttgaaaat
     2881 ctcgtcaaat acttccccat catgaagggc gccttcattc gcaagcaggt tggcgcagtc
     2941 catgcggtgg atggcgtttc ttttgagatc tacaaaggcg aaactctggg cctggttggc
     3001 gaatcgggct gcggcaagtc cactactgga cgcgccattt tgcagcttca ccgccccacc
     3061 tcgggcagtg tgatttttga aggccgcgac ctggtcaaga ccaaaggcga agaactgcgc
     3121 ctgatgcgcc gcaagatgca gatgatcttc caggacccct atgccagcct caacccccgc
     3181 atgacggtgg gcgaaatcat tggcgaaccg gtcctggtgc atggcatcat ggatcgcaaa
     3241 gcctcggaaa aacgtgttga agaattgctc tctctcgtgg gcctcaaccc cgcttacgcc
     3301 aaccgctacc cgcacgaatt ttcgggcggt cagcggcagc gggtgggtgt ggcccgcgcc
     3361 cttgcgctga acccctcgct gatcgtttgc gatgaaccca tctccgcgct ggatgtctcc
     3421 atccaggcgc aggtggtcaa cctgctggaa gacctccaaa agcagcttgg cctcacctac
     3481 ctgttcatcg cccacgacct ctcgatggtt cggcacattt ccaatcgggt agcggtcatg
     3541 tacctgggct cgatggtcga actggccacg cgggatgatg tctactatca cccgctgcac
     3601 ccctacaccc aggccctgct ctcggctgtg cccatccccg acccgattgc tgaagagaaa
     3661 cgacaacggc agatcctgaa aggcgaaatt ccttcaccga tcaacccccc ctcgggctgc
     3721 cgttttcgca cgcgctgtcc gcatgcaacg gacatttgcg cggaaaagcg ccccgaatgg
     3781 cgagaagtta cgccagggca ctttgttgcc tgtcacctcg tgtgacgcca acccaacgga
     3841 aggaggtgaa atttcaggaa gaaagatgat cgactctcta accttacgtt tcacagatcc
     3901 atccaaacct agttctatct ctcaaggagg agaaagacaa tgcgttcaaa gttattcacg
     3961 ttaatcggcc ttctggtgct ggcgagcatg gttttggccg cctgtacccc ccccgccgca
     4021 cagactccca ttgtggagac tgtcatcgtg gaagtggccg gcaccccgca ggtcatcgtt
     4081 gtgacccctg aacccgtcgt tgagacgaaa cccgccgtcc tgcgcatcaa catgggcacc
     4141 tatcccgaca tcattgaccc tcagaaatcc tcgttcgtca atgaaattgc ccacctcaaa
     4201 ctgttctacg aaggtctcac ccgcctcgat caggccctcg agaccgtccc cggtgccgct
     4261 gaatcgtggg aatacaacga agatgcgacc cagttgacct tccaactgcg ccctgacctc
     4321 aaatactccg atggcaccct cctcaatgcc aagcgcttcg aatattcgat cctgcgcaac
     4381 attgaccccg ccaccgcggg cgagtatgcc ggtatcaccg atgaaatcgt cggcgctccc
     4441 gaatggcgca tggccgctgc tgaagacctc gaagccaatg gcgacgccct caaggctgcc
     4501 gtggaagttc gcgctctgga caccgctggc aacgcctgcg tggattacga acaggtcgac
     4561 tgcaccgttc tgcagctcaa gttctccaag cctgctcctt acttccacac ggtcatgtcc
     4621 ctgtgggtca ccttccccgc caaggaagaa aacatctctg aaggtggcga tatctggtgg
     4681 aacagctcca agttccagat tggcaatggc cccttcatca tgaagaccct cgaacccttc
     4741 gtccgcgcgt acttcgtccc gaacgccaac tactgggctg gtaccgccag ctacgatgtg
     4801 gaatacagct tcatcaccga aggcgctgtg gcctttgaag cctacaagaa caatgagttc
     4861 gatatcattg gtctgggcgc cgatgatctg gccaccgttc aggccgaccc ggttctgagc
     4921 accgaagcca acatctaccc cggctcctgc acctacgcct tctacatgaa caaagacaag
     4981 ccccccttcg atgatcccaa agtccgcgaa gccttctcct atggcaccaa ccgcgacgcc
     5041 tgggtcaccg acatcctcaa gggcctcggc gccccgaccc tcacctggat ccccaagggc
     5101 ttccctggct atgatgagac cgaagatcgc tggcagtatg atgtcgaaaa agccaaagct
     5161 accattgccg aatcgaccta tggcagcttc gaggccctcc ccgaaatcac cctgaccttc
     5221 tccggcacct cccgcaacat cccccgcttc gagtggctgg ccaatcagtg gaccaccaac
     5281 ctgggcgtaa aagtcaagct tgacccggtt gaatccacca cctacaccgc cctcaccaag
     5341 gaacgcgaga ccctgcccca ggtattctac ctcggctggt gcgctgacta cgccgatccc
     5401 cagaactggc tctccgtgta ctggatgacc acctctgcct tcgcccagcg ctatggcttc
     5461 agcaatcctg aaatggatgc cctgatgcag gaagctgatg cgactctcga tcccgtcaag
     5521 cgcgctgacc tgtacctcca ggcccagaag ctcctcgttg gcgatgccgc cgtcatcatg
     5581 atggtcaaca acgtcaacag ctatctgatc aaaccgtggg tcaaagacgt caagttcacc
     5641 cccatggatg ccggctggcc tggcgatgtg gatccgctct ccatcaccat cgacacttcc
     5701 ctcatcccct aatcgtggat gatcggcgca aaccgggcta acccctggta agcgccagta
     5761 gaaaacgctg gccggtcttc accctccggt caagatcggc cagcgttccc cctctttgaa
     5821 atttttttag acggtttctc ccggctgccc ggctgggttg ggcagtttat cttcagcggt
     5881 aggagtattt atggtcccct atattcttcg cagactcctg acaatgatcc cggtattgat
     5941 catcgtttcg ggcatcacgt tcatcctgat gcacgcggct cccggcggtc cctgggaccg
     6001 cgaccccggc gcccgccagg tggatgccaa cacccagaaa ctgctcaccg aatactacgg
     6061 tcttgataaa gacccgtttt tccagtggac aacctacacc ttctttggcg aaaacaaaga
     6121 tggcaaagtt gtctgcggtg ccatctgcgg caacctgggc ccctcttacc gccagcgcgg
     6181 catgaccatt cagcaaattc tgtttgctcc acccgaaggc cgcccctggt attacagccg
     6241 ctttggttat tcgctgcggt tggcatttct ggcaatgagc gtagccatcc tgattggtat
     6301 gcccatgggc atcctctcag cgctcaaaca gaacaccggc tgggactatg tcgccatgtt
     6361 catctcgacc ttaggcgtct ccatccccag ctttgtggtt gccatcttct tgattatttt
     6421 gttcaatgaa ctggatttga ttaccattgt gccaaaatcc tgggatagtc tgggcgtctg
     6481 ggtactgccc gcctttgtcc tcggctttgg caccctgtct cgcacggccc gcctcacccg
     6541 cgcctccatg ctggaagtca tccgccagga ttacatccgc accgcgcgct ccaagggcct
     6601 tgctgagggc accgttatca cccgccatat gatcaaaaac gccctcatcc cggttgtgac
     6661 catcctcggc ccgaacctgg ccggtttgat tacaggttcg ttcattattg aaaccatgtt
     6721 ctccttccct ggcatgggcc gcgcctatgt aaccgccatc ggtcagcgcg attattccat
     6781 gatcatgggc accacgctgg tctttgccat cctcatcagt ctggcgaaca tttccgtgga
     6841 tattgtctac ggctttattg atccccgcat ccgcattgac taggagccaa aaatggctga
     6901 aaccccaagt aaaattgtaa cttttgaact ccccgaaaag cggcgcagcc tgtggggcga
     6961 cgcctggtac cgcctggtgc gcaacaaggc ctctgtggct ggcatgatca tcatcctggc
     7021 cttttttctg attgccattt tcgcgggcgc aatttccaag cagaaccctc tcgaagtcca
     7081 cagcggcacc gattatttgc cccccgtctg ggttgagaag ggcatcaatg gcaaacccag
     7141 cagccctgat tatctgctgg gcaccgacac cctcggccgc gatgtgctgg cccgcgtgct
     7201 ttacggggcg cgtgtctcca tggtcgtggg catcgtcccc accctcatcg tcctgattgt
     7261 cggcacgatt gttggcctga ttgctggctt ctttggcggc atctgggata acctgctgat
     7321 gcgcttcacc gatatcgtct ggtctttccc ggacctgctg ttcttcatta ttattatgat
     7381 cgccctgcgc gacactttca tcggcaacct gctgaacggc ctgttcctgc tgtttgccgc
     7441 cctggccatg gtcggctggg tgggcgaggc tcgcgtggtg cgcggtcagg tgctctcgct
     7501 gaaagaaaaa gagtttgtcg aagccgcccg ctgcattggc gccaagaacg gccgcatcat
     7561 gttcaagcat atcatgccca atgccctcgg ccccatcatc atcatgggcg ccatgaccgt
     7621 ccccggcatg atcatcaccg aagccaccct gggcttcctg ggcctgggca tgcgcccggc
     7681 cagcgacccc aacaacctgc ccttctttgt caccagttgg ggcgttctga tgaacgaagg
     7741 caacgccgcc attcatgccc agccctggct gctgctcatg cccgccatct gcgtttcgct
     7801 ggtcgtgctg gccttcacct tcctcggcga tggcctgcgc gatgcgctcg atccccgcat
     7861 gcagggcaca tcctgatcgg ttgatcgctt tcaccgtcac tttgagcggg ccgtcaggcc
     7921 cgctcttttt ttgccccaat cgccgttata ataaccctgt taccgacttt gacacctgga
     7981 gattgccgct catgcgcaga aaaatcgttt ccctgcttgc cttattcctg gccgccagcc
     8041 tgctgctgac cgcctgccgc accaccgaaa tcccggtcgc tccggctgac ctcaccccat
     8101 cccccgcggc tgccacagcc accccggcgc ccacccccac gcccacccca accccccgtg
     8161 tgctgaccat ttgcatgggg caggaaccgt ccaccctcta tatttatggc ggctcgtccc
     8221 gcgccatgtg gtccgtgctg gaagccatct acgacggccc cgtggacacg cgcaactaca
     8281 ctgcccagcc ggtgatcctg caaaaacttc ccaccctaac cgatggtgac gcccgctaca
     8341 cccccgttga tgtcgccttt ggcgccccaa ttgtcgccgc ggatggcagc ctgtctaacc
     8401 tggttgccgg ggtgcgctac ctgcccgcgg gctgcactgc cgatgcctgc gctgtcacct
     8461 gggatgggca gagccccgtc cagatggacg cgctgaccct gacctaccac attgtgccgg
     8521 gcatcaactg gtcagacggc gccccgctca cagccgccga ctcagtctat tcctacaccg
     8581 ttgccgccga ccctctcacg cccatcaaca agcgcctggt ctcgcgcacc gcctcctacc
     8641 tggccctgca agatgatcca cttgccaccg aatgggtcag cattcccggc taccgcacca
     8701 ccaattttac cgaagccttc tggcagccgc tgccgcagca tatcgacagc ctgtactctg
     8761 ccgatgccct gttgagcgcc gaggaggttg cccgttaccc gctgggctgg ggtccctaca
     8821 tcattaaaga gtgggtcacc ggtgggcata tcaccctgga gcgcaacccc aactacttcc
     8881 gcgcggccga aggcctgccg cattacgatc tgctggtctt ccgcttcctg ggcgaaaccg
     8941 ccgatggcaa cctggccgcc ctgcgcaacg gcgaatgtga ttttatcgat cagaccacct
     9001 cactgctgga tggcttccag caggccctgg aatatgaaca ggctgcctcc atcaagctct
     9061 ggctgggcct gggccccgaa tgggaacacc tggactttgg cattcgcccc gccaccctgg
     9121 atgatggcta caatgccttc tctgatgaac gccccgatat cttcggcgat gtgcgcgtcc
     9181 ggcgcgcctt tgccgcctgc ctggatcgtg aaggcctgat ccgtaccttc ctgtacagcc
     9241 gctcagccgt gcccactggt ttgttccccc ccaaccaccc gctctacgcg gccagcctgt
     9301 cgcccgccgc ctatgatgta gcggcaggta gcgccctgct ggatcaggtg ggttggcgcg
     9361 actacgacaa cgcccccgcc accccacgcc aggcctacgg cgtgcccggc gtgcccgacg
     9421 gcacccccct gacggtcacc tatctgaccg ccagcgccct gctgcccaac gctgccgccg
     9481 aatacctggc tgcttcgctg gcgcagtgcg gcattggcgt taccattacc gcgctcacgc
     9541 ccggtcaaat gtacgccgcc ggacccgatg gccctctctt tggccgcaaa tttgacctgg
     9601 cgcagttcag ttgggcctcg ggtgctgaaa cgccctgcta cctctatcag accgaccaga
     9661 tccccaccgc ccagaacggc tgggttggcg ccaatctgac cggttacagc agccctgcct
     9721 acgatgccgc ctgccaggct gcccgcctgt ctgccggcac cgatgccgcc gcggccgcca
     9781 acctggaagt gcagcgcctg ctgtccgaag acctgccggt ggtgccgctc tactaccgca
     9841 tcaagctgac cgccgcccgc cgcgacctgt gcaactacac ctttgatgtc agcgcccgca
     9901 gcgagctgtg gaacctggaa gcctgggatg ccggcccgct ctgcgccccg taaatcaatc
     9961 acccattgcc gcttagacag aatgcgggct tgccccgcat tctgcgtttt aattccgagc
    10021 cctaaaaccg acttttaatc ccgatttgtg atatctgaca cccaaattaa cgtcaaaaag
    10081 cacctcgctc ttaaccggca ttcattgaca ttcttgtcta ttccatctat aattattcca
    10141 atccctatcc gggatttgtg cctttgggga gagcccttca gtgttcatcc ctgcaaagca
    10201 agccctataa agcagtcgaa cctgaatgtc tcaattcccc gtcgtcaccc ttcacaatcc
    10261 aatctgtttt ctgttggagg agaaagaaat atgaagcgca ataccatgtt tttggtgctc
    10321 agcctgatca tcgtcgcctc gatggctctg gctgcctgca cccccacggt cgcaccgacc
    10381 gatgctcccg ttgtgaccga agccccggtt gtcaccgagg ctcctgtcgt aactgaggcc
    10441 cccacggcca tcccgacccc cgaacccacc acccgccacg gcggttggtt cgacgagatt
    10501 gtcatctcgg ttgttgacaa tgccgctgct gttacccaga ttgaagcggg cgctatcgac
    10561 atttatgctg atggcgtttc gtccgacacc ctgcccgaaa ttgaggcagc tggtctgggc
    10621 tactcttcgg cgaacggcct gtactatgaa ttgaccttca accccgttgg ccccgtcttc
    10681 gagtccaccg gcaaagtcaa tccattccac tttgctgcag ctcgcgaagc catgaactgg
    10741 ctgatcgacc gcgattacct caatcgcgaa atctacagcg gtggtgcact ggccaagttc
    10801 ttcccgcttg tcaccaacat gccagactac accagcattg ccccgaccat ccgccggctg
    10861 gaagctgaat atgcttacaa cccggaacgc gccaaggctg ccatggatga ggtcatgctc
    10921 ggcatcgaag gcgtgacgat tgaagatggc aagtacatgt tcaacggcga acaggtcacc
    10981 ctcattctgc tgatccgcaa cgactctgac aagactcgtg tcccgatcgg cgactatgtt
    11041 ggcaaccagc tcgagagcat cggtttcgcg accgatcgcc agtacaagac ctcctcagaa
    11101 gcctccccaa tctgggtcgg tggcaatcct gcggatggcc tgttccacat ctacactggc
    11161 gcctggtccg ccactgccat ttcccgcaac gaagcggaca acttccagtt ctttgacagc
    11221 ccatcttctg cctatggctt cactgctctg tggcaggcca acaacatcga cgaggaatac
    11281 aaggaactcg ccgatgatct ggcctatacc aacttcaaca cccccgaaga acgcctcgaa
    11341 gctctggaac gcgccgtgga actctccctc aagaactcca tgcacgtctg gctgatcgat
    11401 ggtaaacgct tcatccccta caaagatggc ctgaccgtga cctatgacct ggctgccggt
    11461 gttgctggcg cccagatctg gccgtacact cttcgtcagg aagaccagga aggcggccag
    11521 ctgaagtggg gttccccgga cctgttcgtt gaccccatca accccatcgc cggctccaac
    11581 tgggcgttcg atcagcccat tgttcgctcc acctacgacc agagctacat gtacgatccc
    11641 tacactggct tgatctggcc gctgcgcttt gataaagccg aagttaccgc cctggaaggc
    11701 ctgcccattg gtgctactca cgattgggtg accctcgatt tcgctcccga aatcaatgtc
    11761 cccgaagaca ccattgttga ctgggatgcc gtcaaccagc agttcatcac cgctggcgaa
    11821 aagttcccgg aaggcaccac tgccaagatc aagagcattg tctactatcc cgaagccctg
    11881 ttcactgatg tgaaaaatca ggatggtacg acgatggata tcgctgactt cctgtattcc
    11941 tggatcatca gcctcgatat cggcaaagaa ggctcgctca tctatgatga atcaatggcc
    12001 cccaatgtcg aagcttccct ggaaacctac aaaggcttcc gcatcctctc cgaagatcct
    12061 ctaatcatcg aagcctacag cgacaactgg cagatcgatg ctgagatgaa cgtcaccacc
    12121 ctgtggccga ccttctccta cggttatgcc ccgtggcaca ccctggccgc tgcctccctg
    12181 gctgatgcgg ctggtgaagt tgccttctct gcggacaagg ccgatgccaa caccactgaa
    12241 accaagactg ttgaatggct cagcctggtg ggtggcccca gccttgatat catgaccaac
    12301 tacctggatc aggccattgc cgatcagcac atcccctacg ccaatgtcct ttctgcctac
    12361 atcaccccgg atgaagcggt tgctcgttac gaagccgcca agggcttcta cgaagaatac
    12421 ggtcactaca tgatcggtaa cggcccctac atcctgagcg aaatctacct gactgaaaag
    12481 gttgctaccc tgatttccaa tccggactat gttgaccttg cagacagatg gtctggcttt
    12541 gctgcgccgc gcattgcgga tgttgaggtg gatggtgaag ccagtgtcgt gctgggtacc
    12601 gaattcacct ttgatgtgat ggtgaacttt gatggcgaac cctatgcctc tgaagatgtc
    12661 aaagaggtca ttggtttgct ctacgattcc acgggtcaaa tcgtcgaagt gctcaagggc
    12721 gaactggtgg aagaaggtct ctatcgcatt acggttcctg cggaactctc cgcgactatg
    12781 gccgccggct ccagcaagtt ggaagctgtt gtggttccct acgtcgtggc tattccgacc
    12841 ttctatgcat tcgaattcgt gactgtcaag taagtccgat cggattgcat acgggtaact
    12901 aagtgtttgg aggggcaagg tgaatcaatc tccttgcccc tccttatctc gatgaggagg
    12961 taggatatat gggatcggtc gctgaaacca caactcccac gatagagaag aagaaatcca
    13021 aaggcgagag cacccttgtc agggtcttaa agtattctgg attccggctg ttctcccttt
    13081 ttgttacggt tgtcattggc atttacctga caattttgat cgctaacatg gggggctatg
    13141 ttgacaaaat gatggtctct gaaatccagg agcgggttac catgcagatt agcatggacc
    13201 ccaaaaataa gaccatggac ccagaggtca agaacaattt gattcaggaa agaatccagg
    13261 cagaaatggc tcgcctggat ctggaccaac ccgcggccat ccgctcaatc cgatttctca
    13321 agaatgcact gaccctgaac ctgggccgtg caatgtttat gaccagcgat ttgggttccc
    13381 gcaatgtaaa aaacatcatt ctggaacgca ttccttatac gctgttgtta atgggcacag
    13441 caaatctgat tcttttcttt gtcacgctct ttctggcatt ggcactttcc cgcaaatatg
    13501 gtagcttttg ggataagttt gtcattaccc tttcgccgac ttcgtcagta ccgccatggt
    13561 tttatggcat attcttgatc ttgatttttg cggcgatact caaatggttg ccatttggcg
    13621 gcgtgattga tgcaccagtt ccaaaggatt tgatggggcg cattctcagc atcctcaaac
    13681 atctcattct gccggcgaca tctctgattg tctcatcctt cttcctgagc atctacaact
    13741 ggcgcacctt cttccttatc tactccagtg aagattacgt ggacacagcc aaggccaaag
    13801 gtctgtcagc ccgcgatatt gaacgtcgtt acatcctgcg cccaaccctg ccaaccatca
    13861 ttacatcgtt tgctctgatg ttgattggta tgtggacagg tgccattatc actgaaaccg
    13921 tgttcacatg gccaggactt gggcgtgtga cctacaaagc catcggtctg tatgataccc
    13981 ccgtaatcgt tggcacaacg atcctttacg catacctgtt ggcaattacc gtattcctgt
    14041 tggactttgt ctatgcgttg gttgatcctc gagtcaaaat tggtggtggc ggaggcgcaa
    14101 aatgaaaaat tttctcaata ccctccggga aattggcaag tacccaacgg ctatctttgg
    14161 cgtcatcgtg gtgttggcgc taatcattgt cgcggttgtg gtaattatca agatccccta
    14221 tgatcaggcc atcagcacct ggcgcggtgg agaatcgatt gtctataaaa acccccgcaa
    14281 cgtcccccca aaatggtaca actggttccg cgcggaaaaa ctcgttgaat ccttcgatat
    14341 gaaggaaggc gatgaaggtg tctcttcggt tacaaccgta actgaaggtg gaacgacgat
    14401 caccaccttc acgtttgaat tcgatcatag ttacacatcc ttcccccagg acatggttct
    14461 gtacttcaac tcggaatacg agacacaaca gccgtttgtt gccataaaac tattcacgcc
    14521 ggatgaacgg gaaatcaaaa ttgctagttt tgcattaaca aaatcccaca cttatccgat
    14581 gctgcaggat caaaaactgc agaagcgtct caataatgtg gtccccaata tcggcctatt
    14641 cctggatccg gatgtagaag aacagatccc ggtccaggga acctacaggt tggaaataca
    14701 agcaacgatt tttgaatcga attcagtagt tcatcctgag tttgtcttgc atggccaggt
    14761 gtttggctgg gcagggaccg atcatatgcg ccgtgacctg agcctgccgc tcttgtgggg
    14821 tatccccatt gcgctatcgt ttggcttgct ggccgctctg ggcacatccc tcctgaccat
    14881 gatcattgct gcgtttggca cctggtatgg cggtttctgg gatgccctga tccagcgcat
    14941 cactgaggtc aacatggtgc tgcccttcct ttccatcctg atcatggtgg gcacattcta
    15001 ctcccggtcc atctggacaa ttctgggagc aacgattgcg ctctccatct tcaccggtgg
    15061 tattaagagc taccgcgcca tgttcacgca ggtgaaagag tctacctaca tcgaagccgc
    15121 tcgctcttac ggggccggtg atgggcgtat catcttcaaa tacctgatcc ctcgtatgat
    15181 accgttgctt ctgcccgggt tgatttctgc agtcccgtca tttgtattcc tcgaagcctc
    15241 tctggcgctg ctgggaatcg gtgacccggt tctgcccacc tggggcaaga tgatcaatga
    15301 ggcctacggc aatgctgcac tctaccgggg ctggtactac tggattttgg aaccagccgt
    15361 cctgttgatg atcaccggtt tgggctttgc aagccttggt tttgcactcg accgtatctt
    15421 caatccacga ttgcgagggg tgtaaaaatc atgactgaca acttattact ccgtgttgaa
    15481 aatctgaaac tgtactttaa agcttcaaag gggttggtgc aggcggttga tggtgtgaac
    15541 ctcaacctga acaccaatga ggccgtcgtg atcattggcg aatctggctg cggtaaatcc
    15601 tccatggcca aagccctgct ccggctcctg ccgcgcaatg ttggcaccta ttccggtagc
    15661 gtttacatgg atggtgtgga tgtcatggct ttggatgatg aaaaatatcg ccgcgatgtc
    15721 cgctgggtta agatgagcat ggtgcctcag gcagccatga actccctcaa ccccgtactc
    15781 aaagtgggcg atcaggtagc cgaacccctg atggtgcacc acggcgtgtc taaagaagcc
    15841 gccctggaac agtctcgtgc tatgtttcaa cgggtaggcg taccggtaga ctttgtcaac
    15901 cgatatgctt ttgagctgag cggtggtatg cgccagcgtg tagccattgc catgtctctg
    15961 gtcaccatgc ccgacctggt agtcttggat gaacctacct ctgcgctgga tgtgctgacc
    16021 caggctaaca tcatgaacct gctcaagcgc atcaagaaag agatcaacac cagctttatc
    16081 ctcatcaccc acgacattgc cacttcctca gaattggctg atcgtgtagc ggtgatgtat
    16141 gccggacaga ttgttgagac gagcgactca taccgcttct ttactgctcc cctgcacccc
    16201 tactcggcca aattgatggc ttcagtgcca aaactgcgca gcactgccga gccagacttt
    16261 atcaccggcc aaccgccgag cctcatcaac ccgcccacgg gctgccgctt tgcggctcgc
    16321 tgccccaagc gttttgaaaa gtgcggcgaa gaacctccca cggttaccgt ggatggacgc
    16381 ctggtcaagt gctggctgta tcagtaaggc gaggagaaca agatggccaa acaaaaacat
    16441 acccttgaag agttgaaatc tacggtagct agccataccg gtcccttgct ttcgattcgg
    16501 gatctacatg tctggtttga actgcgccgc tttggctttg ggcgtgcggg ctttgttcgc
    16561 gcggtagatg gcgtgagttt caatctggat cgcggcgaag ctgtggctgt ggtcggtgag
    16621 agtggctgcg gtaaatccag tctgatgaaa accattctgg gcatcaacat ccccaccaag
    16681 ggtgacgtgt actatgctga tgtccccatg ggtgaattct cccgttctca gttgaaatgg
    16741 tatcgttcac aggtgggcta tgtgcagcag gacccttatg gcgctctacc gcccttcatg
    16801 aatatccaga ctatcctgga agaacctctg atcatcaacg gcgtcaagag caagcaagaa
    16861 cggctcgaac gcatccacaa ggcgcttgaa gaagtcaaga tgacccctgt ggatgaattc
    16921 ctgcccaaat tcccgcatat gatcagcggt ggtcagcagc agcgcgtggt catcgcccgc
    16981 gccatgatca acaaccccac cctgctggtc gccgatgaac cagtatccat gttggatgct
    17041 tctgtccgtg tggaaatcct caaactgctg cgcggtttgc aggaatctcg taacttagcg
    17101 gtgatctaca tcacccacga cctgtccacg gtgcgctact tctcggaata tattttcgtc
    17161 atgtacgctg gcgaagtgat tgagaaagcc cgtgtggcag atctgattaa taaccccggg
    17221 catccctata cccacgccct ccttgcggcc acctctgacc ccgatgccaa gaacgtggac
    17281 gaattccagg aagtcccccc cggcgaaccc ccgtcactgg tcaagccgcc ggaaggctgc
    17341 cgcttccacc cgcgctgcaa gcacctgatc gatggtctgt gcaataaaga gcgccctcag
    17401 gaatttgaag ccgccccggg gcactttgtt cggtgctggc tgttccaaga aaatggaaag
    17461 agttaacccc aagtattgga tctggattgg gcttgttcta ctgctgattg gcgttgttct
    17521 gccgctgatg atggttgttc gagtgattga agcaaccttc ttcctggtct tcttctctca
    17581 tgcggcatca gtggtcgggg cttttatggg cttctatggc atagccatgg gatacatatc
    17641 cagaaagaac cgccacgaat aaactgaacc gtcatggacg gaactggtga tcagcaatag
    17701 aaattacgag gggcaaatat tgattgaatc agcctggcga ggtgattttc accccgccag
    17761 actgattttt tgcgctataa ttctattacg caatcggggg agcgctcgat tgcagtattc
    17821 tcggaggctt gcggaatgac tcaacgcgtg atgattgtgg atgatgccat ggaactgggg
    17881 cggctgctca aagccgccct ggaggtcctg ggcgcaaaat tgaccatttc agttcatccc
    17941 tcggctgaag aagccatcct ggatgcgggc aaacacaccg tcgatctggt ggtgaccgac
    18001 atgcgcctgc ccggcatgac cggcgtagat ctgatcaagc gcatgcgcaa acgccaaccc
    18061 gacctcaagg tcatcctcat cagcggcctg acagaccgca agatccgcca gcaggctgaa
    18121 gaactgggct gcgaggcctt tttgcccaag ccgctggaaa tggaagaatt tatccgcgtc
    18181 tctgccgccg ccctgggtgt caatccgcct gctctggttg gcagtgatcc cgttgcagga
    18241 gaagaaaccc cggcccccgc gccgcgccga gaaaaagaca aagaaaaacc tgccgagccg
    18301 cctgccgcag atgctttggc cgctgagacg caaaaacagc ttttggaaaa cctgtgtaaa
    18361 tcggcggccg ctctgggcgt tgtcttactg gacgaaaaag ggaaccgcct ggttcgcagc
    18421 ggcgacttcc ccaagattga agtggctggc gaatggattc cccccctgat gaccgtgatg
    18481 cagtcctcaa atcagtttgc caatatgttt gccgatacgc acccttcggt gggcgtcacc
    18541 atgcgcggcg ccgcctttga cctggtgatg gcacctgtga acagcggcgt ggtcatggtt
    18601 gcactcaagc ccggcagcag cagcatccgt ctggcgctgg cgctcgaaga ggtgctggcg
    18661 gttcaacccc gtctggcagc caccctggcc gcatcccctg ccgaagccac cgactctgct
    18721 ccggccaaat ccggctctaa aaccacagcc aaacccgcgc ccgcgcccga accagaaccc
    18781 ccggccgtac ccgaagaccc cgctgccctg gctgcgtttg cgtccctgtt cggtgaaggc
    18841 gctgccccgg cggccccggc tgtatcagac acagattcct tctgggagca ggccgccgat
    18901 caacagcttg ccaaggtcaa ccccgatacc atcagctttg accaggctgt gcagcttggc
    18961 ctggctcccg acgagcccga taaagagaaa taaccgctcc ctgaccggtt tgcgctataa
    19021 tgaatccaga tgtccaatca tctggctgtt ccatttatgc ccggctctgc gcctgtcgcc
    19081 agcctgccgc tggggcgtta cctgcccctg ctgccggtgg gcatggctgc cgcctgggcg
    19141 cgccagcacc tccaacccgg cgactgggtc tttgacccgc tgggcgcaca ccccctcctc
    19201 gccctggaag ctgcccgcgc cggctgtcgg gttctggttg ccagcaacaa ccccatcctc
    19261 accctcatgc tccaggtgct ggctgccgcc ccacaaacga ctgaccttca gtctgcgctg
    19321 agcgatctgg cggctttgcg aaaaggcgaa gagcgtctgg agcttcactt ccaatcgctc
    19381 taccagacgc cctgccccgg ctgcggcgaa ctggttccag ctcaggcgtt cctctggcgg
    19441 gatggtgaag accagcccta tgcccgcctg atcaagtgcc cgcgctgcag tgccgagggc
    19501 gagtttccca ttaccccctc agacctcgag cgcctgacgc cccccggcgc cgcggccctg
    19561 caccgttctc gggcggtgca gcgtgctgcc ctgcccgacg agcccctggg cgaggcggtg
    19621 gaagccgcaa tcaaaaccta cctgccccgc ccgctgtata ttctctcgac attgatcaat
    19681 aaagtcgaag gcctgctcat cccggctgag cggcggcgct tgctctatgc gctgctcatc
    19741 agcgcgctgg atgagggcag tgccctctgg ccctggccgc cgggacgcgc ccgtccccgt
    19801 cagatcaata ttcccccggt ttatcgtgaa aacaacctct ggctcgcatt agagaacgcc
    19861 gtctcggcct gggtcaatgc ccgcaccccc ctgccggtgg tctactggcc cgagcttccc
    19921 ccggagaccg gcggcatctg catctaccgc ggccgcgggc gagagctttt tcccctgccg
    19981 ccaaccatcc acctgagcgc cgttctaacc gttctgccgc gcatccagca ggcgctctgg
    20041 acgctctcgg ccctctggac gggctggctc tgggggtctg acgcgctggt gccgttacgt
    20101 ggcgccctgg aacgccgccg ctacgattgg tctggctatg ccggggccct ggcgcagacc
    20161 tttgccgcgc tccgtccgca cgcccccgca gatttgccgc tgtttgccat tgtccccgaa
    20221 ctcaacccgc cgttttctgc ggctgttctc accgcgctgc aatctgccgg ttttatcctg
    20281 acgggcgcgg ccctcagccc ggaaggtgat ctggcccagt tcacctggca gcctgccgaa
    20341 gcgggtgctt cacccggaac cgtgctggaa gggatgcaag tccatctccg cgagcgcggt
    20401 gaacctgccg attttgccac cacttatctg gctggcctct tgccagcctg tgccccggca
    20461 gccccggccg ataccccacc ccttcagtca gttcagactg cgctggaaaa tgcttttcac
    20521 aattcggcgg tcttttatca ttacaaaacc agcgaaaacg ccgcactgga tacgggtctg
    20581 tggggccttg ctgactctgc cgggtctgac ctaccgctgg cagaccgcat ggagcgtttt
    20641 gtggtcacct ggctgcaaaa aacacccgag tttatgcaga gcgcccttga agctgacttg
    20701 tgggccgaat ttccggggct gctcaccccc ccgcaagact tactgcgggc ctgcctcgag
    20761 tcttacgccg aacccaccgg cgcacccggc gcctggcggc tgcgccctca agaggctgcc
    20821 gcagaacggc gtgcccacct gaaggtggtc tatcaactgc tcacccggtt ggcagagcag
    20881 ttgggctatc cctgttcagg cgagtcgccc ctgatctggc ggggcgccta tgccttcttc
    20941 cccatggcct cagccatcat cagccggttt gtatctgcgc cgcagccctt gcccccggag
    21001 cgctgtgtgc tggtctttcc cggcgggcgc gccagtctga ttaacgtcaa gctgcgccgc
    21061 gatccgcgcc tggctcaggc ggcccgcggc tggcatctgc tcaagtttcg ccatctgcgt
    21121 gacctgtccg ggcgagccaa cctcaccccc gcagcctttg ccgacctgct ggacgccgat
    21181 ccgccggatt gggaagacgc ccgccagatg aagatgttct agccgcggcg atttttgccg
    21241 aagttgtctt gacaacctcc ctaattgcgc gacaataatt aaaacgccgc atcatggccg
    21301 gggtggattt taccggtacc cctgacccac tggcccccca cagataaaca ggaggctgaa
    21361 atggcgcact tcaggggtat tgttttacgg ctgctggtcg cggttgtgct ggtgctgggg
    21421 ttctccccgg ttcatccggt tcaggcttac attccgattt ccggcacggt aacctggaca
    21481 attgaccaga tcattgccac agatgtggtg gtgctcaacg gggccttgct caccatccag
    21541 agcggggtga gcgttaccat gcagtgcggc attgacccgg cgccgtatag cagcggctta
    21601 tccaccaaaa ttgaaatcat tgttgaaaac ggcgggacac taaacatgaa cggcgccatt
    21661 atccatggcg atggcaatgg cggctgctgg caggg
//
LOCUS       DAOPVS010000023        21313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2463_length_21313_cov_56.336156, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000023 DAOPVS010000000
VERSION     DAOPVS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21313
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2463_length_21313_cov_56.336156"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKL11_03790"
     CDS             complement(<1..815)
                     /locus_tag="PKL11_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12755.1"
                     /translation="MQRKSLFVVLAIILIASMALTACTPKPDETNAEPVVFVFGRGAD
                     SVQLDPSIVTDGESFRVTGQVLEPLYQYEPGSTKPIPALAEECTPNEDATVWTCKLRK
                     GVKFHDGTDFNADAVIFNFERWRFTTHPNHYASQVFEYYEAMWGGFDDASIITEVKKI
                     DDYTVEFDLSAPLAPFLANLAMDMFAISSPAAIEKAGESYGTPTGGCVGTGPFKFVEW
                     VEGDHIIVEKNADYWGGAPKIDQILFRVIPDDSARFLALKAGDIQGMEQAVVE"
     gene            complement(1090..3501)
                     /locus_tag="PKL11_03795"
     CDS             complement(1090..3501)
                     /locus_tag="PKL11_03795"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12756.1"
                     /translation="MRYGEFDLEQREYVITDPRTPVKWINYIGTRQFGGFVDHTGGAL
                     ICKDDPTFNRITKYLQQMPASDFKGETLYLRLHTSAGYQVYSPFFVPSLNPLDRFECR
                     VGPGYTRLVTEGCGLRTEVTIFVPPGGLCELRDIQVTNLSSAPLTVDAVPVVEFTHPD
                     ALKQFTNADWVPQTMQCHAAQVDGLTVLLQYPFMHRDRQVNLFSASLPASSYETDRRR
                     FLGENEYGSFRQPLSLLQPELSSQPALRGDTLAALLIPLGTLQPGETRRLITRLGQSP
                     SLAAAESDIRRCRQPQTVAAGLAELRAFWDAYFATFQVATPDAPMNVMLNVYHPRQCY
                     VAKTWSRYLSYYQLGLGSRGIGMRDSAQDLLSVIAAVPAEARDFLSLLLAFQKQDGSA
                     MHQFNPLTLEGSAGDSMEMEDRPHYYSDDHLWLVLAACAYLKETGDLAFLSENIPFYE
                     KDRQGQPLEIAPVLEHLRRAVAFTRGDVGAHGLPLLGFADWNDTINLPRGAESLFSAN
                     LYGRALLELIALYEHMGDTAAADEARAAHAEMRARVESAAWDGAWYVLYFDAEGQPVG
                     SNRNTYGKIHLNGQTWPVLSGFASPERARLALDAVYQHLNTPFGIKLSSPGFNGYDPH
                     YGGVTTYPPGAKENSGIFVHPNPWAMIAEAMLGNGDRAFEYYAQINPAARNDQMEIYE
                     CEPYAYAQNILGDEHPQFGLARNSWLTGAAAWSYQAATQWILGIRPEFSGLRIDPCIP
                     AAWDSFKVTRRYRGSLLHITIHNPDHVCKGVSRLFVDGLLVSDNIIPVNLPPGDHYIE
                     ARMTA"
     gene            complement(3513..>5188)
                     /locus_tag="PKL11_03800"
     CDS             complement(3513..>5188)
                     /locus_tag="PKL11_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNZ12757.1"
                     /translation="VVFLLGYQENPVAEKFDPPASQRINKRTALPIIHQYLQPAAIDA
                     AFADLRRYWDGLLGQLQVQTPDVHTNRMVNIWNAYQCMITFNMSRSASYFESGIGRGM
                     GFRDSNQDLLGFVQMVPASARQRLLDLASTQLPTGGAYHQYQPLTKRGNNEVGGGFND
                     DPLWLVLAVAAYLKETGDWAILDEPTPYDNQTGTEQPLYEHLQRSIQYTLERLGPHGL
                     PLIGRADWNDCLNLNCFSDTPGQSFQTTTNFDGKVAESVFIAGLFVLAAHEMAGIASH
                     CHHPAETAQLRANAEKMSDTVARHGWDGAWFRRAYDNYGNPVGSSACTEGQIFIEPQG
                     ICVMAGLGHGDGRARQALDSVRERLATPHGIVLHQPAYTRYYLHLGEISSYPPGYKEN
                     ASVFCHTNPWIMIAEAQLGNGAAAHDYYLRINPSAREDLSELHGCEPYVYAQTIAGKD
                     APTHGEAKNSWLTGTAAWNYVAITQWILGIRPTLDGLSVAPVVPAEWPGFTAVRQYRG
                     VRYEITVTRRGPSSAIRLEVDGQPVIGSVIPLPPRGVTLVQVQAWVGGE"
     assembly_gap    5189..5288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5289..6118)
                     /locus_tag="PKL11_03805"
     CDS             complement(<5289..6118)
                     /locus_tag="PKL11_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNZ12758.1"
                     /translation="MRYGYFDDPNREYVITRPDTPLPWINYLGCESYFGLISNTAGGY
                     SFYRDARLRRLTRYRYNNAPLDVGGRYIYLRDNATGAVWSPTWQPTQTPLDQYECRHG
                     LGYTTIRSQKTGIEAHLRYFVPLGQNLEVWQLTLTNHRPDPAQLSAFSAIEFCLWDAM
                     DDSANFQRNFNIAELEVEDGVIYHKTEYRERRNHYAFFACSEPLAGFDTQRSAFLGPY
                     HGWNAPQVVSQGQSANSLGIGWAPIGSHHIQLTLAPGASHSVVFLLGYQENPVAEKFD
                     P"
     gene            complement(6250..7200)
                     /locus_tag="PKL11_03810"
     CDS             complement(6250..7200)
                     /locus_tag="PKL11_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12759.1"
                     /translation="MLKLENVSKIYRIGAFGNRELRAVSNVSFEVAPGEVISLIGESG
                     SGKSTIGKMILRLISISGGKITLDGVDISQHRGQALKEYYRLVQGVFQDPFSSYNPIF
                     KADRVFEIVRQEFYPQVSAADWYPRVINALEAVGLNPEHVLNKFPHQLSGGQLQRFLI
                     ARALLLDIKFLVADEIISMLDASTRIDVLNLLASLKSRGLSILFITHDLSLAHYISER
                     AIILYRGFMVEMGATGRIFEHPLHPYTRMLMASVPRLDQKWQEVEIDAVANLPGNGRG
                     CAYYDRCPVASHQCTQSPPPLVEAEPGHFVACFNYSSQSG"
     gene            complement(7193..8155)
                     /locus_tag="PKL11_03815"
     CDS             complement(7193..8155)
                     /locus_tag="PKL11_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12760.1"
                     /translation="MSLQVENLRVYYQTLRGDVKALDGATFSLKNGEIMGLAGESGCG
                     KSTLGNSFVRLAPPMRFVEGKVTLDGQELPIWDQDKMMPHRFRDISIVPQYAMNAMNP
                     TRKIGLMVSELLKTRQVNYAEMLPELHRRLDLVGLPHDVLGMYPIELSGGMKQRMVMV
                     ISTLLNPSLLIADEITSALDVSTQKAVSEMLVEFRDQGFVKSMMVITHDISILYQIAD
                     TIMVMYAGKLAEKAPTETIIHAPRHPYTKLLISSLPKVGVTYTQKKLTGIPGNPPLLL
                     TPPTGCRFRERCPVAYEKCLEEPPFVAIGKNHSIACWREAAPHA"
     gene            complement(8159..9016)
                     /locus_tag="PKL11_03820"
     CDS             complement(8159..9016)
                     /locus_tag="PKL11_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12761.1"
                     /translation="MTKPKKINEFLYFALRNKKLRLSLIVLLFFILLAIFGPLLTEYG
                     PKDFAGPPAQSPNSDYWFGTTTFGEDVFTQFVIGLRATLIVGLVGGGLGTLIGILIGF
                     TAGYRGGLVDEILNVLTNIVLVIPTMAVLLIIAAYLEVRSVATECLFIGLTAWPWAAR
                     AIRAQTFSLRNREFVDLARMSGKKSFNIIFTEVAPNMMSYLVMTFILQFGGAILTAAT
                     LDFIGLGPTNTISLGLIMQYSVLWGALPLEIWWWFVPPGLAITLIVGSLYIMNVGLDE
                     VFNPKLREM"
     gene            complement(9013..10005)
                     /locus_tag="PKL11_03825"
     CDS             complement(9013..10005)
                     /locus_tag="PKL11_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12762.1"
                     /translation="MKRYFLRKILIYLLTFFLAVTIDWMIPRFMPGDPVTNMIARASI
                     NSNAASVMYEYYTHVFGLDLPLWQQYLNFWGSLFRGDLGISVYLFPTPVVDILKRAVP
                     YDLALLLPSILLSWIAGNKFGALAARSKWLDNTVSPLSYILTATPYMWLGILLAWFFG
                     IVLGIFPIAGAYSFSLSPSFSFAFIWDVLKHWFLPFASLFLVGFGGWAIGMRNMIIYE
                     LESDYSHYLTALGAPTKLIRKYAFNNAILPQITGLALQLGVVVAGALVTEVVFSYPGI
                     GYLLLAAIQNQDYFLVQGCFLFIIIGVLLANFIIDIAYVLVDPRTRAGMQGESA"
     gene            complement(10170..11981)
                     /locus_tag="PKL11_03830"
     CDS             complement(10170..11981)
                     /locus_tag="PKL11_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ12763.1"
                     /translation="MRKSIMLALGVLIALSLVLSACTTPAPVVTDAPTDAPVVTEAPT
                     EAATEAPTEAPVATEAPAAVTEYPRNETLYVSGAAWGPASTWNPFQPGSLANTTGTIG
                     FVYEFLFSYDPMSGQMTPWLAESGTWKDANTYDVTLREGLTWSDGQPLTADDVKFTFE
                     LGQQYAALWFAPVWNYLKSVTVIDATHLEFVFENCLYQEWDNNLYNIPIVPKHIWETK
                     TEEDITAGANEKPIGSGAYMYLTHAEDRNAWVRNENWWGIKEFGLPAPKYIVDVRTSS
                     NNVAMGMVLKGELDLSNNFLPGIATLADKGYISTYYAGAPYMLSANTAVLFLNTTKKP
                     LDDAAFRRALAFAIDTPSIVNIAYANLVKAADPTGLLPTLGRFSDPDVVAELGYTFDT
                     EKAKTLLAEAGYVDTDGDGFVEAPDGSKIELQVTCPSGWSDWMAAIDIIATSAQAAGI
                     NIQAATPDYGAWNTALQSATFDMTLNNWASMSNTPWTLYNLLFNHPIREQMQSGNFGK
                     YDNQEMFDLVDALAAIPTTDEAAMKEACAQIQTLMLTDMPMIPLWYNGLWAQFSTSTW
                     TNWPTETSANQTLPTTWSGYWQLGGLMTLINLKPANP"
     gene            complement(12123..13145)
                     /locus_tag="PKL11_03835"
     CDS             complement(12123..13145)
                     /locus_tag="PKL11_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ12764.1"
                     /translation="MSDLTLEDIARLAGVSRSTVSRVINNHPSVRGSVRTRVLEVIQN
                     TGFHPNLAARALVSKRSWMLGLVVPRSVSSFFTDPYFPFLTQGIAQACNQHNFTLGFF
                     LVSTKEDEDKIFPRVSRKGYLDGIIVQSGQIGEGLIDRLNNADIPLVIAGRPLQPNNL
                     SYVDVDNVTAAAIAVNHLIGLGYARIATITGPVDNTVGIDRRAGYEQALAQHGLPVEA
                     DLMIAGDFTEPGGYAAMKKLLPARPRAVFAASDGMAIGAMRAIQSVGLRVPQDIAVVG
                     FDDLPMPTPPTPLLTTMRQPIQEFGARAVEVLIDLIENGNLPPRKVIMTAELIIRDSC
                     GSAQMH"
     gene            13409..14470
                     /locus_tag="PKL11_03840"
     CDS             13409..14470
                     /locus_tag="PKL11_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ12765.1"
                     /translation="MKKKVTISDIAEQSGVSPSTVSLILNNKPGVSDETRERVLDVAA
                     ELGYPVRPLTSPNGSNTLLTVGMIVKIDRDILPQANPFYSKVIMGIEDACRRSGINLL
                     FSSLPVDENNRLLEIPPLMYNESVDGLLLVGVSMDETFAASAGRRTPPIVLVDGYSST
                     NSYDTVVSDNFGAAYQAVEHLIALGHREIGLAGSCEHCFVSLRERRNGYLRALKDNGI
                     SRPCVANFNINKTKGFEEITTLLQDNPQLTALFCVNDDVANAALRAAHALGRRVPEDL
                     SVIGYDDTYIAANTRPALTTLHVDTVAMGRAAVRLLALRLETPDASRMTLTIHPTLVE
                     RESVGVAPKLPLAKESIIE"
     gene            14467..15426
                     /locus_tag="PKL11_03845"
     CDS             14467..15426
                     /locus_tag="PKL11_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNZ12766.1"
                     /translation="MTYPPLPNIPWEERPADCSDVVWRYSGNPIIPRNLIPSANSIFN
                     SAVVPFKGNFAGVFRCDNRKREMNIHRGFSPDGIHWTLEDDPIQWQYADPELSRFEHR
                     YDPRVVWLEDRYYVTWCNGYHGPTIGIGYTFDFETFHFLENALLPFNRNGVLFPRKIN
                     GRYMMLSRPSDNGHTPFGDIFISQSPNLVHWGEHRFVMGVKGGWQSTKIGAGPIPIET
                     PQGWLLIYHGVLTSCNGFVYSFGAALLDLDEPWKVIARGEPYLLSPQTLYECVGDVPN
                     VAFPCATLYDEPTGRVAIYYGGADTVTALAFAKMDELLGFLGF"
     gene            15576..16523
                     /locus_tag="PKL11_03850"
     CDS             15576..16523
                     /locus_tag="PKL11_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ12767.1"
                     /translation="MKKLVQILLILAVLVLSGCTAKPVETQPLKVAVLPVLDSLPLYV
                     AESQGYFDEAGIAVELVPVGSAPERDQLMQSGQIDGMLTELVTTLNYNRNEAQIKIVR
                     FARVATAEYPVFRILAAADSGITTVEDLKNVQIGISEGTVIEYMTDRVLANAGLTPAE
                     ISKIAVPKIADRSALLESGELKAAVMPDPLASLLMQKGAVLVIDDTSLPEVSNSVYAF
                     SIETLDARPEDVAAFMTAVEKAVVDINADKAKYQDLMVELKLVPAALVGSYPIPTFPT
                     ASVPSEAQFNDALAWVMDKGMVTVELKYSDNIDASFLPK"
     gene            16533..17309
                     /locus_tag="PKL11_03855"
     CDS             16533..17309
                     /locus_tag="PKL11_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ12768.1"
                     /translation="MIRAVGLTFHYPKRPPIFQNFDWTVAQGQTWAIIGASGCGKSTL
                     LYLLAGLRQPDAGKMLINDQPIQGPRPQTGLILQDYGLLPWATLRQNVTLGLDLRRFY
                     GPDGRHAPIEGLPGNPEQRVTHWLERLGIAGVANQYPGQVSGGQRQRAAIARTLVLEP
                     DLLLMDEPFASLDAPTRENLQSLILQLHTERPLTTVLVTHTIEEAMTIGEKILVLANR
                     TNTTPLILDNPYAGQIPAVQDEGYTKLTARMRAELKETTA"
     gene            17306..18061
                     /locus_tag="PKL11_03860"
     CDS             17306..18061
                     /locus_tag="PKL11_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12769.1"
                     /translation="MKRLQLVWASLGLLAVWQLLAMIVQRDILPAPLAVLGAFFSEVG
                     GELGGHFLASLWRVIASLLLSIALAAPAGLVLGQSRRLNAFFSPLVYILYPIPKVVLV
                     SIVMLFLGINDLSKIVMIFLILFFQILVLVRDQAAAIRPELIYSVRSLGAGRRGLFKY
                     VYLPASLPAILTAVRQSIGTAVAVLYIVELIATTRGLGYYIYLYGTTLYDYPKMYAGV
                     VAMSLLGLGLYFAADWLHRKLCPWQFQTSEDAA"
     gene            18058..18774
                     /locus_tag="PKL11_03865"
     CDS             18058..18774
                     /locus_tag="PKL11_03865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNZ12770.1"
                     /translation="MKPPQGEERTRYVRRMFARIAGRYDLLNRLMTFGQDRAWRTEAI
                     QHLAVQPGQRILDAGSGTGDIALQIVQQTPGVRVVAADLTPEMMAIGRQRPNGERVVW
                     MVADAQHLPFCDGAFDGVISGYLLRNVPDVDAALAEQYRVTGDEGQMVSLDTTPPRRN
                     ILLPFIRFYLKVIIPLLGKLVAGDSEAYTYLPETTAQFLTAEALAMRITQAGYIVDGF
                     ARRMLGSMAIHWGRKTLQKP"
     gene            complement(18771..19679)
                     /locus_tag="PKL11_03870"
     CDS             complement(18771..19679)
                     /locus_tag="PKL11_03870"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNZ12771.1"
                     /translation="MENAAPKPLPHWRRWWLAIRPRTLPAATAPVLIGLAVAFSQGYF
                     KWLPALVILLCALLLQILANLVNDVADFQKGTDKSGRLGPTRVTQSGLLTPRQVWTGA
                     VGVALLAMLGGVYLVFVGGWPVLVMGAASILGAVLYTVGPYALEDYGLGDLFALIFFG
                     FVAVCGTTYILAGQLFPLSWVGGLGAGSLVTDILVVNNVRDIESDRRSGRKNIPVCWG
                     RRAGEIEYLIMLILAYLAPILAIILGWADLWLLLPLLSLPRAIFLWQQIRTLPAGPGF
                     NRLLAQTAQLVLLYCLLFSAGVLLSR"
     gene            complement(19711..21123)
                     /locus_tag="PKL11_03875"
     CDS             complement(19711..21123)
                     /locus_tag="PKL11_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ12772.1"
                     /translation="MKRSLQGKLIFSYLVIALITVLVVSALIRLTSGQSLMNLVMEQQ
                     TAMLNEAVQSYYTENATLDGFFDYYLQQNRRDNPPPTQPDGIPDKGPGMGDIRGVHGL
                     VDLEYRALMPTFNYEIGQIVPADLIKQKIAVEVDGQTIAWILPDTSFQFKLSAEEQLF
                     LKRTNLAIGLAALAGVLAAVAMGFFLSSRLLKPIRRLTQASQALAHGDLQQQVPVTSQ
                     DELGQLTATFNQMSADLTRADQQRKRLTADITHDLSTPLQIISGYIEMLEDGEVTLTP
                     QRIDIIKTEIEHLRRLVGDMTTLTQVEAGALEITLQPVSPAALLQRIYQAYQPIAARS
                     GVELILEQSEPLPFIQVDEGRMLQVLKNLVDNALRYTPAGGSIILSAAADARVQLRVK
                     DTGTGIEPEDLPYVFDRFYRADKARSGSSGKLGLGLAICKALVMAQGGTIAAESAGKH
                     QGATMVITFAPVAEPSSNPG"
     gene            complement(21120..>21313)
                     /locus_tag="PKL11_03880"
     CDS             complement(21120..>21313)
                     /locus_tag="PKL11_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ12773.1"
                     /translation="SPGQVVRRAELAMRLMENGYSGSEATLKIHIRNLRLKLDDDLDQ
                     PRYIETVFGVGYRFMEAAE"
BASE COUNT         4780 a         5389 c         6706 g         4338 t
ORIGIN      
        1 tcttcgacca cagcctgttc catgccctgg atgtcgccag ccttgagggc caggaagcgg
       61 gcagagtcat cggggatcac gcggaagagg atttgatcaa tcttcggtgc gccgccccag
      121 taatccgcat tcttctcaac gatgatgtgg tcgccttcaa cccattcaac aaatttgaag
      181 gggccggttc ccacgcagcc gccggtgggc gtgccatagc tttcaccagc tttttcgatg
      241 gcagccgggc tggagatggc gaacatatcc atggcgaggt tcgccaggaa gggcgccagt
      301 ggcgcgctca gatcgaattc aacggtatag tcgtcaattt tcttgacttc ggtaatgata
      361 ctggcgtcat cgaaaccacc ccacatcgcc tcgtaatatt caaagacctg gctggcgtag
      421 tggttggggt gcgtggtgaa gcgccagcgc tcgaagttga agatcacagc atcggcattg
      481 aaatcggtgc catcatggaa tttgacgcct ttgcgcagct tgcaagtcca tacggttgca
      541 tcttcattgg gcgtgcattc ttcagccagg gccgggattg gtttggtgct gccgggctca
      601 tactggtaga gcggctccag cacctggcca gtgacgcgga acgactcgcc atcggtcacg
      661 atggacgggt caagctggac ggaatcggcg ccccgtccga agacgaagac aacaggttct
      721 gcattggttt catcgggctt gggggtacag gccgtcagag ccatgctggc gatcaagatg
      781 atggccagca caacaaacag ggatttgcgt tgcataactc tctcctctag acttggtgaa
      841 aaacgaaaga aaatacgaca aattcggtca ggcgagttat ctgatcgcgc gcacctcctt
      901 ctctgaattg ttagtaaaca agggggaaca aagtaattat atatgagaac aagacaatca
      961 ggtcaagcga tttagattaa agtttgctca taaagcgagt caattaaata caaaaacaac
     1021 ttcgcctggt tttaatggac gaagccagca gtcccccact acaaagttct cagcctaaat
     1081 cattggtcgt tacgcggtca ttcgagcttc aatgtaatga tcacccgggg gcaaattgac
     1141 agggatgata ttatccgaca ccagcagacc atcgacaaac aggcgggaaa cgcccttgca
     1201 aacatggtcg gggttatgga tggtgatatg aagcaggctg ccccgatagc ggcgggtgac
     1261 tttgaaacta tcccaggcgg cggggatgca gggatcaatg cgcaagccac tgaactccgg
     1321 gcgaatgccc agaatccact gggtggcagc ctgataactc cacgcggcag cgccagtgag
     1381 ccaggaattg cgcgccaggc caaactgcgg atgttcatcg ccgaggatgt tctgggcata
     1441 ggcataaggc tcgcattcgt agatttccat ctgatcgttg cgggcggcag gattgatttg
     1501 agcgtagtat tcaaacgccc ggtcaccatt gccgagcatg gcttcggcaa tcatagccca
     1561 ggggttgggg tgaacaaaga tgccgctgtt ttctttggcg cccggcgggt aggttgtcac
     1621 gccgccgtaa tggggatcat aaccgttgaa accgggggaa gagagcttga tgccaaaggg
     1681 agtgttcagg tgttgataga cggcatccag ggcaaggcgg gcgcgttctg gcgaggcaaa
     1741 gccggagagc accggccagg tttgcccgtt gagatggatt ttgccataag tgttgcgatt
     1801 tgagcctacg ggctgacctt cggcatcaaa ataaagcaca taccaggcgc cgtcccaggc
     1861 ggcagattcg acgcgggcgc gcatttcggc atgggcagcg cgggcttcat ctgctgcggc
     1921 ggtgtcgccc atgtgttcgt agagggcgat gagttccaat agggcgcggc cgtagaggtt
     1981 ggcagagaac agcgattcgg cgccgcgggg caggttgatg gtatcgttcc agtcagcaaa
     2041 gccgaggagc ggcaaaccat gagcgcccac atcgccacgg gtgaaagcca cagcgcggcg
     2101 taaatgttcc aacacgggag cgatttccaa cggctgaccc tgacgatctt tttcgtagaa
     2161 tgggatattt tcagacagga aggcaagatc gccggtttct ttgagatagg cgcaggcggc
     2221 caaaaccagc cagaggtgat catcgctgta gtagtgcggg cggtcttcca tttccatgga
     2281 atctccggca gagccttcca gcgtcagcgg gttgaactgg tgcatggctg agccatcctg
     2341 cttttgaaag gccagcagga gcgagagaaa atcacgcgct tcagcgggaa cggcagcaat
     2401 gaccgagagt aggtcctggg cagaatcgcg cataccgatg ccgcgggagc ccaggcccag
     2461 ttggtagtaa gacaggtagc gggaccaggt tttagccaca tagcactggc ggggatgata
     2521 gacattcagc atgacgttca tgggggcatc gggcgtggca acctgaaagg tggcgaagta
     2581 ggcatcccag aaggcgcgta gttcggcaag gccagccgcc acggtctggg gctggcggca
     2641 gcggcgaatg tctgactcag cggcggcaag gctgggggat tggcccaggc gggtgatcag
     2701 gcggcgggtt tcgccgggct gcagcgtgcc aagggggatg agcagcgcgg caagggtatc
     2761 gccgcgcagg gcgggctggc tgctcaactc cggctgcagg aggctgagcg gctgccggaa
     2821 gctgccatat tcattctcgc caagaaaacg gcgacggtca gtttcataag atgaggctgg
     2881 caggctggcg gaaaagagat tgacctgccg gtcacggtgc ataaaggggt actgcagcag
     2941 gacagtcagc ccatctacct gggctgcgtg acactgcatg gtctggggca cccagtcggc
     3001 attggtgaac tgcttcaggg catcgggatg ggtaaactcc accacgggaa cagcatccac
     3061 cgtaaggggg gcagaggaca ggttggtgac ctggatatcg cgcagttcgc acaggccgcc
     3121 cggtgggacg aagatggtca cctcagtgcg caggccacag ccttcagtca caagacgggt
     3181 atagccaggg cctacccgac actcaaagcg gtccagcggg ttgaggctgg gaacaaaaaa
     3241 cggtgagtag acctggtaac cggcagacgt atgcaggcgc aggtagagcg tctcgccttt
     3301 gaaatcagag gcgggcattt gctgcaagta tttggtgatg cggttgaagg ttgggtcatc
     3361 tttgcagatc aacgcgccgc cagtatggtc aacaaagccg ccaaactggc gcgtgccaat
     3421 gtaattgatc cacttgacgg gggtacgggg atcggtgatg acgtattcac gctgttccag
     3481 gtcaaattca ccgtagcgca tgggctgctc ctttactcgc caccgaccca ggcctgaacc
     3541 tgaaccagag tgacaccccg gggcggtagt ggaataacgc tgccaatgac cggctggcca
     3601 tccacttcca gacgaatggc gctgctgggg ccccggcggg tgacggtgat ttcatagcgt
     3661 acgccgcggt actgccggac agccgtgaag ccgggccatt cggccgggac aaccggagcg
     3721 acggataatc catccagcgt ggggcggatg cccaggatcc actgggtgat ggcgacataa
     3781 ttccaggctg cggtgccggt cagccaggaa tttttggcct cgccgtgggt gggagcatct
     3841 tttccggcga tggtctgggc ataaacatag ggctcacagc cgtgcagctc gctgaggtct
     3901 tcgcgggcag agggattgat ccgcaggtaa taatcatggg cggcagcacc attgccgagc
     3961 tgcgcctcag cgatcataat ccaggggttg gtgtggcaga agacactggc gttttccttg
     4021 tagccgggcg gataggagga gatttcgccc aggtgcaggt agtagcgggt gtaggcgggc
     4081 tggtgcaaga caatgccgtg cggggtcgcc aggcgctcac gcaccgagtc cagagcctgc
     4141 cgggcgcggc catcgccatg gcctaacccg gccatcacac agatgccctg cggttcgatg
     4201 aaaatctgac cttctgtaca ggcgcttgag ccgacaggat taccgtaatt atcataggcg
     4261 cggcggaacc aggcaccatc ccagccatgc cgggcaacgg tgtccgacat tttttcggca
     4321 ttggcgcgca gttgagcggt ttcggcgggg tgatggcagt gactggcgat cccagccatt
     4381 tcatgggctg ccagcacaaa cagcccggcg ataaagacgg actcggcgac tttgccatca
     4441 aaattggtgg tggtctggaa ggactgcccg ggcgtatccg agaagcagtt gaggttgaga
     4501 cagtcgttcc aatcggcgcg gccaatgagc ggcagcccgt gggggccaag gcgctcgagg
     4561 gtgtactgga tggagcgctg caaatgttca tagagcggct gctcggtgcc ggtttgattg
     4621 tcgtaaggcg tgggttcgtc cagaatggcc caatcgccgg tttccttcaa gtaggcggca
     4681 actgccagca ccagccagag gggatcatcg ttgaagccgc cgccaacctc gttgttgccg
     4741 cgtttggtca ggggttggta ctggtgatag gcaccgccgg tgggaagctg ggtggatgcc
     4801 aggtcaagca ggcgctggcg agcgctggca gggaccattt gaacaaagcc gagcaaatcc
     4861 tgattggaat cacggaagcc catcccccgc ccaatgccgg actcgaagta cgacgctgag
     4921 cgggacatgt tgaaggtgat catgcactgg taggcattcc agatgttgac catgcggttg
     4981 gtatgcacat caggggtttg cacctggagc tggccgagca ggccatccca atagcggcgc
     5041 agatctgcaa aggcggcatc aattgcggcg ggctgcaggt actgatgaat gatgggcagg
     5101 gccgtacgct tgttgatgcg ctgagaggcg gggggatcaa atttttcggc aacagggttt
     5161 tcttgatagc ccagcagaaa gaccacggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnngg atcaaatttt tcggcaacag ggttttcttg atagcccagc agaaagacca
     5341 cggaatggga cgcgccgggc gccagcgtca attggatatg atgcgagcca attggcgccc
     5401 agccaatgcc cagcgagttg gcagactgcc cctggctgac gacctggggg gcattccaac
     5461 cgtgataggg gcccaagaag gcgctgcgct gggtatcaaa accggccagc ggttctgaac
     5521 aggcaaagaa ggcataatgg ttgcggcgtt cgcggtattc ggttttgtgg tagataacac
     5581 cgtcttcaac ttccagttca gcgatgttga aattgcgctg gaagttggcg gaatcatcca
     5641 tggcatccca gaggcaaaat tcaatcgcag aaaaggcgga aagctgagcc gggtcgggac
     5701 ggtgattggt gagggtgagc tgccagactt ccaggttctg gcccaggggc acaaaatagc
     5761 gcaagtgggc ttcaatgccg gttttctgcg agcggatggt ggtgtagcca aggccatggc
     5821 ggcattcata ttgatccaac ggcgtctggg tgggctgcca ggttggcgac cagaccgcgc
     5881 cggtggcatt atcccgcagg taaatgtagc gcccgccaac atccagcggg gcgttgttgt
     5941 agcggtaacg ggtcagacgg cgtaagcggg catctcgata aaaggaatag ccgccggctg
     6001 tattggagat gaggccaaaa taagactcgc agccgaggta attgatccag ggcagggggg
     6061 tgtctggacg cgtaatcaca tactcgcggt tgggatcgtc aaaataaccg tagcgcattg
     6121 tggctcctgt tactattatt tgaagatgtc cccgcagggg cggtcaccgg agccgcccct
     6181 gcaaggcgga ggagaaagca gacccataag cggcagagaa gcaagccggg ggcatctgct
     6241 gattataggc taacctgatt gagaactgta gttaaaacag gcaacgaaat gacccggttc
     6301 agcctcaaca agcggcggcg ggctctgtgt acactggtgg ctggcaaccg ggcaccggtc
     6361 atagtaagca cacccacgcc cgttgccggg caggttggcg acagcgtcaa tttcaacttc
     6421 ctgccatttc tggtcaaggc ggggcacaga ggccattaac atacgggtgt aggggtgcag
     6481 cgggtgctca aagatacgcc cggtggcgcc catttcgacc atgaagccac ggtacaaaat
     6541 gatggcacgc tcactgatgt agtgcgccag agagagatcg tgggtgatga acaggatgga
     6601 caggccgcgg gatttgaggc tggccagcag gttcaacaca tcaatgcggg tggaggcatc
     6661 gagcatgctg atgatttcat cagccaccag aaatttgata tcgagcagga gcgcacgggc
     6721 aatgagaaaa cgctgtaact gaccgccgct gagctggtgg gggaatttgt tcagcacatg
     6781 ctcgggattg agccccacgg cctcgagagc gttgatgacg cggggatacc aatcggcagc
     6841 actgacctgg ggataaaact cctggcgcac aatttcaaac acccggtcag ctttgaagat
     6901 gggattgtag gagctgaatg ggtcctggaa aacgccctgg accaaacgat aatactcttt
     6961 gagcgcctgg ccgcgatgct gcgagatatc gacaccatca agggtgattt tgccaccgct
     7021 gatggagatg agccggagga tcatcttgcc gatggtgctc ttgccgctgc cgctttcgcc
     7081 aatgagcgaa atcacttcgc cgggagcaac ctcaaagctc acattgctga ctgcgcgcag
     7141 ttcacggttg ccaaaagcgc cgatgcgata gattttggag acgttctcta atttaagcat
     7201 ggggggcagc ctcccgccaa caggcgatgg aatggttttt gccaattgct acaaacggcg
     7261 gttcttcaag gcatttttca tacgcaacgg ggcaacgctc gcggaagcgg caaccggtgg
     7321 gcggggtgag gagcaggggc gggttgccgg ggatgccggt taacttcttc tgagtgtagg
     7381 taacgccaac tttgggcagt gacgagatca gcagtttggt gtagggatgc cggggggcat
     7441 ggatgatggt ttcggtgggg gctttttcag ccagcttgcc cgcatacatg accatgatgg
     7501 tgtcggcaat ttgatagaga atggaaatgt cgtgggtaat gaccatcatg cttttgacaa
     7561 agccctggtc acggaattcc accagcattt ctgaaacagc tttttgcgtg gaaacatcca
     7621 gcgcggaggt gatttcgtca gcgatcagca gcgaggggtt aagcagggtg gagatcacca
     7681 tgaccatgcg ctgcttcatg ccgcccgaaa gctcgatggg atacatgccc agaacatcgt
     7741 gcggcaagcc caccagatca aggcggcggt gcagttcggg cagcatttcg gcgtaattga
     7801 cctgtcgggt tttcagcagc tcagaaacca tgaggccaat tttgcgggtc gggttcatgg
     7861 cattcatggc gtactgaggc acaatggata tgtctcgaaa acggtgcggc atcattttgt
     7921 cctggtccca gatgggcagt tcctgaccat ccagggtgac tttgccttcc acaaaacgca
     7981 tggggggcgc cagacggaca aagctgttgc ccagcgtcga tttgccgcag ccggattcac
     8041 cggcgaggcc cataatctca ccatttttca ggctgaaggt ggcgccgtcc agggctttca
     8101 cgtccccgcg cagggtttga taatacactc ggaggttttc tacttgtaaa ctcatgggtt
     8161 acatctcccg cagcttgggg ttgaagactt cgtcgagacc aacgttcata atgtaaaggc
     8221 tgccaacgat cagagtgatg gcgagacccg gcggaacaaa ccaccaccag atctccagcg
     8281 gcaaggcgcc ccataaaacc gagtactgca tgatcaggcc gagggaaatg gtattggtcg
     8341 ggccaagacc gatgaagtcg agggtggcgg cagttagaat ggcgccaccg aactggagga
     8401 tgaaggtcat gaccagatag gacatcatgt tgggggcgac ttcggtgaag atgatattga
     8461 aggacttttt gccgctcatg cgcgccaggt ccacaaattc acggttgcgc agagaaaagg
     8521 tctgggcgcg aatggcacgc gcagcccagg gccaggcggt gaggccgatg aacagacatt
     8581 cggtcgccac actgcggact tcgagatagg ccgcaataat gagtaagacc gccatggtgg
     8641 ggatgaccag cacgatattg gtcaggacgt tgagaatttc atccaccagg ccgccgcggt
     8701 aaccggctgt aaaaccaatc aggatgccga tgagggtgcc aaggccaccg ccgaccaggc
     8761 ccacaatcaa cgtggcgcgc aggccaatca caaactgcgt gaagacatct tcaccaaagg
     8821 tggtggtgcc gaaccagtag tcactattag gagattgggc tggcggaccg gcaaaatcct
     8881 tggggccgta ttcggtaagc agcgggccaa aaattgccag caggataaag aatagcaaga
     8941 cgatcaggct gaggcgcaat tttttgttgc gcagtgcaaa gtacagaaat tcattgatct
     9001 ttttgggctt ggtcatgctg attctccctg catgccggcg cgggtgcgag gatccaccag
     9061 cacataggca atatcaatga taaagttagc caacagcaca ccgatgatga taaagaggaa
     9121 gcagccctgg accaggaaat aatcctggtt ttgaatggca gccagcagca gataaccaat
     9181 tccgggatac gagaacacca cctcggtcac cagggcacca gcaaccacca cccccaactg
     9241 taacgccagg ccggtaattt gcggcaggat ggcattgttg aaggcgtact tgcggatcaa
     9301 ttttgtgggt gcgcccagag cggtgaggta gtgagaatag tctgattcca gttcataaat
     9361 gatcatattg cgcatgccaa tggcccagcc gccaaaacca accaggaaga gcgatgcaaa
     9421 tggcaagaac caatgcttca aaacatccca aataaaggca aatgaaaaac ttgggctgag
     9481 tgagaagctg taagcaccgg cgatggggaa aattcccaga acaataccaa agaaccaggc
     9541 cagcaggatg cccagccaca tgtagggggt ggcggtgagg atgtagctga ggggcgaaac
     9601 cgtgttgtcg agccacttgg agcgggccgc cagagcgccg aatttgttac cggcaatcca
     9661 gctcagcaga atggagggca gcaacaaggc cagatcatac ggaacggcgc gcttcagaat
     9721 atccaccacg ggggttggga agagatagac gctgatgccg aggtcgccgc ggaataatga
     9781 gccccagaag ttcagatact gctgccagag gggcaggtcc aatccaaaaa cgtgggtgta
     9841 atactcatac atcaccgagg cggcgtttga attgatggag gcccgggcga tcatgttggt
     9901 tacggggtct cccggcatga agcgcgggat catccagtcg atggtgaccg ccagaaaaaa
     9961 ggtcaaaaga taaatcagga tctttctcaa gaagtaacgt ttcacgaaag ttcctccccg
    10021 gacgggtaag atgctggggc gtacaataag taagatggtg ggggctggga tgcgccggga
    10081 ttgatgaatg gatgattcgg ttgccggcgc atcccggtaa gggccgcccc tcccaaccga
    10141 acgggagggg cggcaggaag aaatcagatt tacggatttg ccggtttgag gttgatgagg
    10201 gtcatcagac caccgagctg ccagtaccca gaccaggtgg tgggcagggt ctgattggca
    10261 gaggtttcgg tcggccaatt cgtccaggtg gaggtgctga actgagccca caggccgttg
    10321 taccacaggg ggatcatggg catgtcggtc agcatgaggg tttgaatctg ggcgcaggct
    10381 tccttcatgg cggcttcgtc agtggtgggg atggcggcga gggcatcgac caggtcaaac
    10441 atttcctggt tgtcatactt gccgaagttg ccgctctgca tctgttcacg gatggggtgg
    10501 ttgaagagca ggttgtagag cgtccagggg gtgttgctca tggaagccca gttgttgagg
    10561 gtcatatcga aggtggcact ctgcagggca gtgttccagg cgccatagtc gggcgtggca
    10621 gcctgaatgt tgatgccggc agcctgggcg ctggtggcga tgatatcaat cgcagccatc
    10681 cagtcagacc agccgctggg gcaggtgacc tgaagttcga tcttggagcc atcgggagct
    10741 tcaacgaagc catcaccgtc ggtatcgaca tagccagcct cggcaaggag ggtcttggcc
    10801 ttttcggtat cgaaggtgta accaagctca gcaacgacat cggggtcaga gaagcggccg
    10861 agggtgggga ggaggcccgt gggatcggca gccttgacca gattggcata ggcaatgttg
    10921 acgatggagg gggtgtcgat ggcgaaggcc agggcgcggc ggaaggcggc gtcatccaac
    10981 ggtttcttgg tggtgttgag gaacagtacc gcggtgttgg cagagagcat gtagggggcg
    11041 ccagcgtagt aggtgctgat atagccctta tcggcgaggg tggcgatgcc gggcaggaag
    11101 ttgttgctca ggtcaagttc gcctttgagg accatgccca tcgcaacgtt gttgctggag
    11161 gtgcggacat caacgatgta cttgggagcg ggcaggccaa actctttgat gccccaccag
    11221 ttttcgttgc gaacccaggc attgcgatct tcagcatggg tcaggtacat gtaggcacca
    11281 gaaccaatgg gtttttcatt cgcgccagcg gtgatatctt cttcggtctt ggtttcccag
    11341 atgtgcttgg ggacaatcgg gatattgtag aggttgttgt cccattcctg atagaggcag
    11401 ttttcgaaaa cgaattcgag gtgggtggca tcgatgacgg tgacactctt caggtaattc
    11461 caaaccgggg cgaaccagag ggcggcgtac tgctggccca gttcgaaggt gaatttgaca
    11521 tcgtcggcgg tcaggggctg gccatcagac catgtgaggc cttcgcgcag ggtcacatca
    11581 taggtattgg cgtctttcca ggttccgctc tcggcgagcc agggggtcat ctgaccggac
    11641 atgggatcgt aggagaagag gaactcatac acaaaaccga tggtaccggt tgtgttggca
    11701 aggctgccgg gctggaaggg attccaggtg gaggcagggc cccaggccgc gcccgagacg
    11761 tagagggtct cgttgcgggg gtattcggtc actgcggcgg gagcttcagt ggcgacgggg
    11821 gcttcggtgg gggcctcagt ggcagcttcg gtaggagctt cggtaaccac aggggcatcg
    11881 gtgggggcat ccgtgacaac gggtgccggg gtggtgcagg ctgacagcac caggctgagt
    11941 gcgatcagaa caccaagggc gagcatgatt gacttacgca ttttctttct ccttttagga
    12001 atattggggg aattttggaa aagggcaggg gaaacttttt gaaagatcgg atgtaatacg
    12061 gtcacctcct tatgaaaagc cggattgctt ttcatgtacc tcggcggggg gcgcaaacca
    12121 ggctagtgca tttgcgccga cccgcacgag tcccgaatga ttagttcagc ggtcatgatg
    12181 accttgcgag gcggcaggtt gccgttttcg atcaggtcaa tcaggacctc aaccgcacgc
    12241 gcaccgaact cttgaatggg ctggcgcatg gtggttaaca aaggtgtggg gggtgtgggc
    12301 atgggcagat catcaaagcc cacaacagcg atatcctgcg gaacccgcag cccgacggat
    12361 tgaatggcgc gcatagcgcc aatggccatc ccgtcagagg cggcaaagac ggcccgcggg
    12421 cgggcaggca ggagtttttt catggccgcg tagccgccgg gttcggtaaa atctccggcg
    12481 atcatcagat cggcttccac aggcaggccg tgctgcgcca gcgcctgctc atacccggca
    12541 cggcggtcaa tgccgacggt gttatccacg gggccggtga tggtggcgat gcgggcatag
    12601 cccaggccaa tcagatggtt gacggcgatg gcagcggcgg tcacattatc aacatcgaca
    12661 tagcttagat tattcggctg gaggggacga ccagcaatga ccagcggaat atcagcgttg
    12721 ttcaggcgat caatcaagcc ttcgccaatc tggccggact ggacaatgat gccatccaga
    12781 tagcccttgc gagatacacg ggggaagatt ttgtcctcat cctctttggt gctgaccagg
    12841 aaaaacccga gggtgaagtt gtgctgattg caggcctggg caatgccctg ggtgaggaag
    12901 gggaaataag gatcggtaaa gaaggagctg acactgcgag gcaccaccaa cccaagcatc
    12961 caagagcgct tggaaaccag ggcgcgggca gccaggttgg ggtggaagcc agtgttttga
    13021 atgacttcca gcacgcgtgt gcgcacactg ccccgcacgc tggggtgatt gttgatcacc
    13081 cgcgagacgg tggagcggga aacgcccgca agtctggcaa tatcttccaa agtgagatcg
    13141 gacacgcagt ggctccgaga gggggatttg aaatctgggt ggtgctgcgc atttgggagc
    13201 gctcccatat ttcaaaaata ttataaaatg tttttattaa agagtcaaca ggttttaacg
    13261 gcaatttagt aactaaacct taaaatttca gcgctttttc tgatttagca agcaaatggg
    13321 ggttttagct tgacaggagg ccgttaactg ctaaaatata aaaatattcc agtaaatatt
    13381 tcagtaaaac aatcgggagc cggaagcctt gaagaaaaaa gttaccatca gcgatattgc
    13441 cgaacagagc ggggtctccc cttccacggt ctctctgatt ctgaataaca aaccaggggt
    13501 ttcggatgaa acccgcgaac gcgtgctgga tgtggctgcc gagctgggct acccggtacg
    13561 accattgaca tcgcccaacg gcagcaatac cctgctgacg gtgggcatga ttgtcaaaat
    13621 tgaccgggat atcctgccgc aggccaaccc gttttattcc aaagtgatta tggggattga
    13681 agacgcctgc cggcgcagcg gaatcaacct gctgttttcc tcactgcccg ttgacgagaa
    13741 taaccgtctg ctggaaattc cgccgctgat gtacaacgag agcgtggacg ggctgctgct
    13801 ggtgggtgtg agcatggatg agacatttgc tgccagcgcc gggcgccgga cgcccccgat
    13861 tgtgctggtg gatgggtact ccagtaccaa cagctatgat acggtggtct cggacaactt
    13921 tggggcagct tatcaggccg tggagcattt gattgccctg gggcaccggg agatcgggtt
    13981 agccgggagc tgcgaacact gttttgtcag cctgcgcgag cgccgcaatg gctatctgcg
    14041 ggcgctgaaa gataacggga tcagccgccc ctgtgtggca aacttcaaca ttaacaaaac
    14101 caaggggttt gaggaaatta caaccctgct gcaagacaac ccacagctaa cggcgctgtt
    14161 ctgcgtcaac gatgatgtgg caaatgccgc actgcgggca gctcatgcgc tggggcggcg
    14221 ggtaccagaa gacctgtcgg tgattgggta tgatgatacc tatattgccg caaatacgcg
    14281 cccggcgttg accaccctgc atgtggacac ggttgccatg gggcgcgcgg cggtgcggct
    14341 gctggcgctg cgcctggaga cgccagacgc ttcccgaatg actctgacaa tccacccaac
    14401 cctggtcgag cgcgagtcgg tcggggttgc gcccaaacta ccacttgcaa aggaatcaat
    14461 catcgaatga cttatccgcc cctccccaat attccatggg aagaacgccc ggctgactgt
    14521 tcagatgttg tctggcggta cagcggcaat cccatcattc cacgcaacct gatcccctcg
    14581 gccaacagca ttttcaacag tgcggtggtg cccttcaagg gtaattttgc gggggtgttc
    14641 cgctgcgata atcgcaagcg agaaatgaac atccatcgcg gcttcagtcc ggatggcatt
    14701 cattggacac tggaagacga ccccatccaa tggcagtatg ccgacccgga attaagccgg
    14761 tttgaacacc gctatgatcc ccgggtggtt tggctggaag atcgttacta tgtgacctgg
    14821 tgcaatgggt accatgggcc gaccatcggg attgggtaca catttgattt tgagaccttt
    14881 cactttttag aaaacgccct gctgccgttt aaccgcaacg gagttttgtt cccgcgaaaa
    14941 atcaatgggc gttatatgat gttgagccgt cccagcgata acggacacac tccgtttggg
    15001 gatatcttca tcagccagag tcccaattta gttcactggg gcgaacaccg ctttgtgatg
    15061 ggggtcaaag gcgggtggca gtccaccaag atcggggcag gaccgattcc gattgagaca
    15121 ccgcagggct ggctgctgat ttaccatggg gtgctgacct cgtgcaacgg gtttgtgtac
    15181 agctttggcg cagcgctgct ggacctggat gaaccctgga aggtgattgc gcgcggcgag
    15241 ccttatctgc tctcaccgca aacgctgtat gagtgtgttg gcgatgtgcc caatgtggct
    15301 ttcccctgcg ccacgctcta cgatgagccg actggacggg ttgccatcta ttacggcggc
    15361 gccgataccg tgaccgcgct ggcatttgcc aaaatggatg aactgctggg attcctggga
    15421 ttctaggcag acagcgagat ccaggatttt tgtataacag gtccgccttg tgcgggcctg
    15481 tttttttgga ttctatgggt cattattgtt ataatggcgc tcagagtaag tgataaatct
    15541 agacaaaaat aatcgatgat taacgaggtc agacgatgaa aaaattagtt caaatcctgc
    15601 tgattctggc agttctggta ctgagcggtt gcacagccaa accagttgaa acccagcctt
    15661 tgaaggtggc ggtgctgccc gtattggatt cgctgccgct gtatgtggct gaaagccagg
    15721 gctattttga cgaggcgggc attgcggttg aactggtgcc ggtggggtcg gcgccagagc
    15781 gcgatcaatt gatgcagtcg ggtcagattg atgggatgtt gacggaactg gtgacaacgc
    15841 tgaattacaa tcgcaacgag gcccagatca aaattgtgcg ctttgcccgt gtggcgacgg
    15901 cagaatatcc ggtctttcgg attctcgcgg cggcagacag cggcataaca accgttgaag
    15961 atcttaagaa cgtgcagatt gggatttctg agggcacggt gattgaatat atgaccgacc
    16021 gggtgctggc gaatgccgga ttgacgcctg cagagattag taaaattgcc gttccaaaaa
    16081 ttgctgaccg ctcggcactg ctggagtcgg gggaattgaa agccgccgtg atgcccgacc
    16141 cactggcatc gctgttgatg caaaaaggcg ctgtgctggt gattgacgac accagcctgc
    16201 cagaggtgag caacagtgtg tatgccttca gcatcgagac actagatgcc agaccggaag
    16261 acgtggcggc ctttatgacc gcggttgaaa aagcagtggt tgatattaat gcagataagg
    16321 ccaagtatca ggacctgatg gtggaattga agctggtgcc tgccgcgctg gtgggcagct
    16381 accccatccc caccttcccg acagccagtg tgcccagcga ggcacagttc aatgacgcac
    16441 tggcgtgggt tatggataag gggatggtga cggttgagtt gaaatacagc gacaatattg
    16501 acgccagttt tctgccaaag taagccaaca agatgatccg ggctgttggc ctgacgtttc
    16561 actaccccaa acgcccgccc attttccaga actttgactg gacagtggcg cagggacaaa
    16621 cctgggctat cattggtgca tcgggctgcg gcaagtccac cctgttgtat ttgctggcgg
    16681 gattgcggca gcccgatgcc ggaaagatgc tgattaatga ccagcccatc cagggaccgc
    16741 gcccgcagac cggattgatt ctgcaggatt acgggctgct gccctgggcc acactgcgtc
    16801 aaaatgtgac gctggggctg gacctgcggc gcttctacgg accggatggg cggcatgccc
    16861 cgattgaggg cctgccgggc aatccggagc agcgcgtgac acactggctg gaacgactgg
    16921 ggattgccgg tgtggctaat cagtatccgg gacaggtatc gggtgggcag cggcaaagag
    16981 ctgcaattgc gcgcaccctg gtgctggagc cggatctgct gttgatggat gagccgtttg
    17041 cttcgctgga tgcgccgacg cgcgaaaacc tgcaaagcct gatcttgcag ctgcacactg
    17101 agcgcccgct gaccaccgtg ctggtgacgc acaccatcga agaagccatg actattggcg
    17161 agaaaattct ggtgctggca aaccgtacga ataccacccc gttgattctg gataacccct
    17221 acgccggaca gattccggcg gtgcaggatg aaggttatac caagctaacg gcgcggatgc
    17281 gggcagaatt gaaggaaacc accgcatgaa acgtttgcag ttggtgtggg cttcgctggg
    17341 actgctggca gtctggcaat tattggccat gattgtgcag cgcgatatct tgccggcgcc
    17401 gctggcggtg ctgggggcct tcttcagcga ggtgggcgga gaactgggcg ggcattttct
    17461 ggcttcgctg tggcgggtga tcgcctcgct gctgctctcg attgccctgg ctgcaccagc
    17521 cgggctggta ttgggacagt cgcggcggct gaatgcgttt ttctcgccgc tggtgtatat
    17581 cctctatccc attcccaagg ttgtactggt ctcaattgtg atgctgtttc tggggatcaa
    17641 cgatttatcc aaaattgtga tgatcttttt gatcctgttc tttcaaattc tggtgctggt
    17701 gcgcgatcag gcggcggcaa ttcgcccgga gctgatttat tcggtgcgca gcctgggagc
    17761 cggacggcgc gggctgttca agtatgttta tctgcctgcc agcctgcctg ccatcctgac
    17821 ggcggtgcgc cagtcaattg ggacggcggt ggcggtgctg tatattgtgg agctgattgc
    17881 caccactcgc gggctgggct actatatcta cctttatggc accacgcttt atgattaccc
    17941 caagatgtat gccggggtag tggcgatgag cctgctgggg ttgggtttgt attttgcagc
    18001 cgactggctg caccgcaaat tgtgcccctg gcaatttcaa acctcggagg acgctgcatg
    18061 aaaccacccc agggtgaaga acgaacccgc tatgtgcgcc gcatgtttgc ccggattgcc
    18121 gggcggtatg acctgctcaa ccggttgatg acgtttgggc aagaccgcgc ctggcgcacc
    18181 gaggcaattc aacatctggc ggtccagccg gggcagcgga ttctggatgc cggcagcggc
    18241 acgggggaca ttgccttaca gattgttcag cagacgccgg gagtgcgcgt ggtagctgcc
    18301 gacctgacgc ccgagatgat ggcgattggc cgacagcgcc ccaacgggga acgggtggtg
    18361 tggatggttg ccgatgccca acacctgccc ttttgtgatg gtgcgtttga tggggtcatc
    18421 tccggttatt tgctgcgaaa tgtccccgat gtggatgcgg cgctggcaga gcagtatcgg
    18481 gtgacgggcg atgaagggca aatggtctcg ctggatacca ccccaccccg gcgcaatatc
    18541 ctgctgccgt ttatccgatt ttatctaaag gtcatcatcc cgctgctggg caaactggta
    18601 gccggggact cagaagccta cacctatttg ccggaaacga cagcgcagtt tctgaccgcc
    18661 gaggcacttg ctatgcgcat tacgcaggca gggtatattg ttgatggatt tgcgcggcgc
    18721 atgctgggca gcatggcgat tcactggggc cggaaaacac tccagaagcc ctagcgactg
    18781 agcaagaccc cggcagaaaa gagcaggcag tacagcaaaa ccaactgagc ggtttgcgcc
    18841 agcaggcggt taaaaccggg gcccgcaggc aaagtgcgaa tctgctgcca aaggaagata
    18901 gcccggggca ggctgagcag cggcagcagc agccacagat cagcccagcc aaggatgatc
    18961 gccaggatgg gtgcgaggta ggccagaatg agcatgatca ggtactcgat ctctccggcg
    19021 cgccgccccc agcagaccgg gatatttttg cgtccggagc ggcggtctga ttcaatatcg
    19081 cggacgttgt taaccaccag aatatcggtt accaaggaac cagcgcccag ccctcccacc
    19141 cagctcagcg ggaagagctg cccggcgagg atataggttg ttccacagac tgccacaaaa
    19201 ccaaaaaaga tcaaagcgaa gagatcgcca aggccgtaat cttccagggc ataggggccc
    19261 acagtgtaaa gcaccgcgcc taaaatagac gcagcgccca tgaccagcac aggccaaccc
    19321 ccgacaaaaa ccagatacac acctcccagc attgccagca gtgccacccc tactgcaccc
    19381 gtccagacct ggcggggcgt gagcagaccg gattgcgtca cgcgagtggg gcccaggcgc
    19441 ccgctcttat cggttccttt ttgaaaatcc gccacatcgt tgaccaggtt ggcgaggatt
    19501 tgcagcagga gggcgcagag caaaatcacc agggcgggca gccatttaaa atagccctgg
    19561 gagaaggcaa ccgcaaggcc gatcagaacc ggggcggtgg cggcaggcag ggtgcgcggg
    19621 cgaattgcca gccaccaacg gcgccaatgg ggaagaggtt tgggggcagc gttttccatg
    19681 gggagaatta tacccaggga tagaaatttt tcaaccgggg ttgctgctgg gttctgcgac
    19741 aggagcaaag gttataacca tggtggcgcc ctgatgctta ccggctgatt cagcggcgat
    19801 tgtgccaccc tgggccatca ccagtgcttt gcaaattgcc aggcccagcc ccagcttgcc
    19861 ggaactgccg ctgcgggctt tatcggcgcg gtagaagcgg tcaaagacat agggcaggtc
    19921 ttcgggctcg atgccagtgc cggtatcttt gacccgtaac tgcacccggg cgtcagcagc
    19981 agccgagagg ataatcgagc cgcccgcggg cgtgtaccgt aaggcattat ccaccaggtt
    20041 cttcaacacc tgcaacatgc gtccctcatc cacctggatg aatggcaagg gttccgattg
    20101 ttccagtatc agttcgaccc cgctgcgggc agcaatgggt tgataggcct ggtaaattcg
    20161 ctggaggagc gcagcaggcg aaacgggctg gagggtgatt tccagtgcgc cggcttccac
    20221 ctgagttagc gtggtcatgt cgcccacaag gcggcgcaag tgttcgattt cggtcttgat
    20281 gatatcaatc cgctgcgggg ttaatgtgac ttcgccatct tccagcattt caatgtaacc
    20341 ggatatgatc tgcagcgggg tgctcaaatc gtgggtaata tcggcggtca ggcgcttgcg
    20401 ctgctggtcg gcccgagtca gatcggcgct catttgattg aaggtggcag tcaactggcc
    20461 gagttcatcc tgggaggtga cgggcacctg ctgctgcagg tcaccgtgtg ccagggcctg
    20521 cgaggcctgc gtcaggcggc ggatgggctt gagcaggcgg ctggacagga agaagcccat
    20581 ggcaaccgcg gccagcaccc ccgccagggc ggccaggcca atggcaaggt tggtgcgctt
    20641 cagaaaaagc tgttcttcag cgctgagctt gaattgaaag ctggtgtctg gcaaaatcca
    20701 ggcgatggtt tgtccatcta cttcaacggc aattttctgc ttaataagat cggcggggac
    20761 gatctgaccg atctcgtaat tgaaggtcgg catcagagcg cgatattcca gatctaccag
    20821 gccgtggacg ccacgaatgt cgcccatgcc ggggcctttg tcgggaatgc catcgggctg
    20881 ggttgggggc gggttgtcgc ggcgattttg ctggaggtaa taatcgaaaa aaccatccag
    20941 ggtggcgttt tcagtatagt aggattgcac tgcctcattc agcatggctg tctgctgttc
    21001 cataaccagg ttcatcaagg actgcccgga cgtgaggcgg atgagggccg aaaccaccag
    21061 cacggtaatc aatgcgatga ccagatagga gaagatcagc ttgccttgca aagagcgttt
    21121 cattcagccg cctccataaa acggtagccc acgccaaaga ctgtttcgat gtaacgcggc
    21181 tgatcgagat catcgtccag tttgagacgc aggttgcgga tgtgaatttt gagggtagcc
    21241 tcggagccac tgtaaccgtt ctccatcagg cgcatggcca gttcggcgcg ccgcacaacc
    21301 tgaccaggag act
//
LOCUS       DAOPVS010000024        19946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2732_length_19946_cov_58.458800, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000024 DAOPVS010000000
VERSION     DAOPVS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19946
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2732_length_19946_cov_58.458800"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKL11_03885"
     CDS             complement(<1..335)
                     /locus_tag="PKL11_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12774.1"
                     /translation="MFRFSGNIRQGVVIALLIVLLLTAAMPAAPVNAASVPIPTFKIV
                     SVVPGISVTIETKNFPANMDFTVRIGEYGTYAIGGYVVATTNSGTGGVFQTTYTIPDA
                     LKNNATLAIR"
     gene            complement(444..575)
                     /locus_tag="PKL11_03890"
     CDS             complement(444..575)
                     /locus_tag="PKL11_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12775.1"
                     /translation="MNSLPWMVGGYIVMLGLLVIYIITLLVRARRLKTAICARKKQL"
     gene            complement(586..1290)
                     /gene="ccsA"
                     /locus_tag="PKL11_03895"
     CDS             complement(586..1290)
                     /gene="ccsA"
                     /locus_tag="PKL11_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNZ12776.1"
                     /translation="MMEQSEIHPGQRTQRILDIAAALAVLGALAVVIFYAPLEATMGA
                     VQKIFYFHLSAAWAGMLSFMIAAIAGGIYLARRQTRWDQLSLSAVEVGLVLMLMAIVG
                     GSIWARQAWNTWWTWDPRLTTVTIMELVYLAYFLLRQGVDDPERRARFGAVYAVVGFL
                     SVPLMYLSIHLYRTIHPLLLGSTANDGMQLAPQMTQTLLISLAAFSVLLAALLYRRVR
                     LARLADTLDALRQQMD"
     gene            complement(1318..2001)
                     /locus_tag="PKL11_03900"
     CDS             complement(1318..2001)
                     /locus_tag="PKL11_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNZ12777.1"
                     /translation="MNRRPRFWRAALTVAWKDLLTEWRSREAFSAMLVFALLVIFIFN
                     FALELKPAARAEISAGILWVTLCFAGALGLNRAWVSEKDRGCLDGLLLAPVDRAALYF
                     GKLFSGWLILMLIAAVLLPIYGLLYNQNLILPGLLLFIALGVEGYLAVGTLLAAMAVQ
                     ARSRDLLLPVLLFPVTLPALIAAVKASSALLSGLPWVDVQPWLMLLLAFDLIFTVVAW
                     LAFDIVMEE"
     gene            complement(1998..2702)
                     /gene="ccmA"
                     /locus_tag="PKL11_03905"
     CDS             complement(1998..2702)
                     /gene="ccmA"
                     /locus_tag="PKL11_03905"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNZ12778.1"
                     /translation="MIEARNLVRRYGYRTILRDLNFTAAPGEFVALLGPNGAGKTTLL
                     RVFAGLLRPTSGVVTVAGCLLPHQAMDLRSRLGVVSHQPLLYGDLTAAENLRFYGQLY
                     GVAHLAARIEALLALTGLEAVRNDLVRTFSRGMQQRLAIGRAMLPDPQVLLLDEPHTG
                     LDQDACVMLDQALSQVAGQGRTLLMASHDLARSAALASRVDILVRGQIIASTATAALP
                     PDGLLTYYRQSLEAAQ"
     gene            complement(2695..4560)
                     /locus_tag="PKL11_03910"
     CDS             complement(2695..4560)
                     /locus_tag="PKL11_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNZ12779.1"
                     /translation="MKLASDGVCKNSWWGVMVALLIPALLSACTPTPMPLIVDPQPSA
                     SPSQTAVASGPYLLPDATNGGQLYAHLCQDCHGQANESLGPQVVALDQPLRPFNHMSV
                     ARTSRPADWFDAITSGIPSGEMPSYGTSLTERQRWDLVAYLFMQNTDAASLALGETIY
                     ERRCETCHGTSGAGDGDKALKSGLTPPNWTIPANLSSYSAMELKESIAQGLAPSMPAF
                     ANRLSEYELRSVAGYVQSFLFGWPQSLSIVSVSPAATPMATLDIEGSVTNGSQNGLPA
                     DLEIALNAYQGSQNVYSAAQPLNDLLGGTYRFDNVPLHPDWIYQVSVIYSNYTFQSDP
                     LSGNGIASSADVNLPLTIYETTTDRSALSAERLQVVVDYSRSGWVQVVQMYLLVNATN
                     RLVVAEQPGDPVLWFDLPDQAENLQFADQITAGRFSILGADFGDYQAIPPGTNYQIVF
                     AYDLPFKSGDVINIHPPVPVSMALIMLPKEVFSLESDQLEDAGERVVEGAQLQLYRAY
                     DLPLSSDLSFSLRERSGMKIQSNSWINLLIGSLMLIIALLITIAWLRATRRPLAALED
                     PPQTPEAILDAIIALDDQARAGKISNSAYHQRRTTLRQQLRAADSAENESSINHD"
     gene            complement(4573..5052)
                     /locus_tag="PKL11_03915"
     CDS             complement(4573..5052)
                     /locus_tag="PKL11_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ12780.1"
                     /translation="MDLGSLFLIIAIALLAGIWIIRPFFQPQAGEALVVDTALHPDIV
                     IRRNLIAERERILAAIQELDSDSALGKIAPNEYTTQRNHLLVTGASVLQRLETVESTL
                     PPQDTSLFSPTEDSALTERLAARRAARQETAAGFCPQCGRPVQQSDRFCARCGKTLS"
     gene            complement(5065..5622)
                     /locus_tag="PKL11_03920"
     CDS             complement(5065..5622)
                     /locus_tag="PKL11_03920"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ12781.1"
                     /translation="MSAALPSKTSPRRISWAVIVAFILLVGFLVLLGISLSRAQSGPP
                     EVGDTAPEFTLTTFDGQTISSTDLAGKVILINFWASWCSPCAEEAVDLEQAWREYQPT
                     GNVIFIGVDYVDTEPEALAYLNEYDITYPNGPDLGTQISRAYRITGVPETYLIDASGV
                     VRGVKIGPFTSLAEIQQFVGSAYAP"
     gene            complement(5622..6128)
                     /locus_tag="PKL11_03925"
     CDS             complement(5622..6128)
                     /locus_tag="PKL11_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNZ12782.1"
                     /translation="MKHTAYTGLISLALTAGLLALAFLLPPPAALAQQPTPSDDAVNA
                     IASQLYCPVCENVPLDVCGTQACAQWRDLIRQKLADGWNEDQIREYFLTQYGDQVVGA
                     PPPRGLNILAYVLPPLAFLIGLFLVLRRFAPRAPVNQPAPIVSDEAIARLEAELQQRK
                     ADASMEDE"
     gene            complement(6125..8155)
                     /locus_tag="PKL11_03930"
     CDS             complement(6125..8155)
                     /locus_tag="PKL11_03930"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNZ12783.1"
                     /translation="MLISNLGFGLLIITLIVAIYSGIAAMIGVTRNARVWAESARAAA
                     VLTFPLLSLSMLALAGLIVAGDYQVQYVYNVASNSMPFYLKLTAIWGGQSGSLLFWCW
                     LLGLSAFLVALRSWHSDQDLLPWIILVIAITLIFFLSLVIFVENPFVRFWRTAGGSEM
                     VALFQPAGALPLIPLDGRGMNPLLRHPGMVFHPPALYLGFVGFIIPYAYAIAALITNR
                     SDDQWIRATRRWSLVAWLFLSLGLLLGSRWAYDVLGWGGYWGWDPVEVAALLPWISGT
                     AFLHSIMMQEKRGLSKRWNMILMILTYSLVIFGTFLTRSGVFSSVHAFSQSAIGPLFF
                     GFIALTLLISLVLLALRWRNLGADTQLQSPFSREGLFLMNNLVMMGLLIACLWGVIYP
                     LISEIFTGQQVTMGPGYYESVAGPLFALLLLLKGIAPLTTWGAGSSRSLGKRLLWPAL
                     GSVVVVIVLLLFGVRQVAALVGLGFAALVIIATVLEYSRAVRGRSLRLKEALPHAFWR
                     LTNLNRRRYGGFIIHLGVALMAVGIIGIELYQTQTQATLQNGQSVSLAGYTVTLQSIS
                     SWDSNDGRNVIAANLTVNRGARFIKQSTPRSDYFYDAQQTMTVPGVYSTLAGDLYIIL
                     ADWNPIYTNQATFKIYYNPLINWLWIGGLIFMLGTLVAAWPPRKSTPLAEVA"
     gene            complement(8165..8650)
                     /locus_tag="PKL11_03935"
     CDS             complement(8165..8650)
                     /locus_tag="PKL11_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNZ12784.1"
                     /translation="MRAKYIIGGLLILAAMVFLIVSATQKNSQYFMTVDELLQQSQQM
                     QGRSVRISGAVLGDTIYYDPNTLTLSFTIVSIPGDNDEIESQGGLAAVLHAAVQDPAR
                     TRLKIEYSGVRPDQLKNEAQAIVTGTLRADGVFEAEELLLKCPTRYEESIPQQAETAV
                     P"
     gene            complement(8816..10069)
                     /locus_tag="PKL11_03940"
     CDS             complement(8816..10069)
                     /locus_tag="PKL11_03940"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ12785.1"
                     /translation="MDTPKRLLILTADAGFGHRSAANAVAAALAQQYGDRCEVTIINP
                     IDDPRVSPLLRESAADYDRIVRLSPQLYQLGYDASDYMVPSAIMESTLTLLLYEVMRD
                     LVDTHQPDAILTTYPLYQIPLLSVFAINRRHIPLVTTVTDLATVHRTWFVSRVDACVV
                     PTPIVQNLALNYGLAPHQIHPLGIPVNPLFAAPRLPKAELRTRLGWQPDLLTVLAVGS
                     RRVDRLVDALNVVNHFGTPLQIIAVAGKDAELFAELRSMEWHVPAHIYEFASNIPEMM
                     LAADCILCKAGGLIVTESLAAGLPLLLVDAIPGQETGNAAYVVENGAGEQVQSPLEVL
                     QTLSHWLGNQHALLDARTRSAAVLGRPRAALDVADLLWSLAQRGPEDLTRRRILERSS
                     LIELLTSNHIPWDIHPVLSKLTQPK"
     gene            complement(10096..10593)
                     /locus_tag="PKL11_03945"
     CDS             complement(10096..10593)
                     /locus_tag="PKL11_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNZ12786.1"
                     /translation="MEIQVGDWLRQCSLKLVVAESCTGGLIGHRITNVPGSSEYYLGS
                     ITAYSYGVKQRLLGVRPETLLAHGAVSRQTVLEMARGVRQSLGGECPLDEVIGLSVSG
                     IAGPGGATPDKPVGLVWIGISAPAGDFAWHFIGQGNRQENKSFSADLALQLLLEVLQG
                     QPRPE"
     gene            10712..12256
                     /locus_tag="PKL11_03950"
     CDS             10712..12256
                     /locus_tag="PKL11_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12787.1"
                     /translation="MKPQKLILVLLVVLALTMRLIPGPRTIDDSYITFRYARNLLAGE
                     GFVYNPGVPVQGTTTPLYTLLMTGLGALAGGTQADFPVIAWLFNAAADAAACILLWLI
                     GKRVRHELAGLGAALVWSVAPYSVTFSIGGLETSLYVLLLTGLAYAYISQKRVLTGLL
                     GALALLTRPDALIFLGPLMLDRFIRAVRPGLLGVKTEPERLQLGEVLAFVIPVAAWYG
                     FATLTFGSPLPHSVQAKLLAYHLEAHEALIRLIQHYATPFIEQSTLGTKIAVGLGLVL
                     YPFFYILGARRVFAAERRLLAWMLFPWLYFITFAIPNPLIFRWYLTPPLPAYFFFVLA
                     GAGSLFKLNGDLRTALRRSWIAALIILLLPVGLSLRAWQLHPDHGPDRPAPGMAWFQL
                     ELLYREAAEFVAPQMNADTVLAAGDVGVLGYYTPARILDTVGLNSEEALSYYPLDEAY
                     YVINYAVAPQLILDEQPDWVIILEVYGREGLLKNETFQQHYTLVKKLETDIYGSDGML
                     IFQRAR"
     gene            complement(12299..12913)
                     /locus_tag="PKL11_03955"
     CDS             complement(12299..12913)
                     /locus_tag="PKL11_03955"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNZ12788.1"
                     /translation="MSSFPHPFYRWRPHPWHGLEIGPKPPEIVHAYIEITPFDLVKYE
                     VDKETGYLRVDRPQRSSSQPPTLYGIIPRTYCGVRVQRLSPQSQRGDGDPLDICVISE
                     RPITKSEVVLNARVIGGIQMIDNGEADDKIIAVLANDSFWDSAQDIENLPPVLVERLR
                     HYFGTYKLTPGKENVTFIAGTYDRQAAYTVVEAAIADYEEAFGS"
     gene            complement(12958..14001)
                     /locus_tag="PKL11_03960"
     CDS             complement(12958..14001)
                     /locus_tag="PKL11_03960"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNZ12789.1"
                     /translation="MPDLSFRNTLHPEPELTAPVDLCDSAGRLNRAAVGWSRQPLHNC
                     NVSGHWPRKKRWNYWCITSPDCLFSATVSNIDYLGMAFIYFLDFHTLKFAEQTVSIPL
                     GKGFELPPTVTGRIHYQNPKLQIAFMDAGNQIDIEVLSQDLGGKMLTTALNACRPAGH
                     ETLNVVIPWDADHFQFTSKQNCLPVSGSLTFDSQEYKFSPDAAFACLDYGRGIWPYAS
                     KWNWASFSTCQNGRQIGVNLGAKWTDDTGMTENGILVDGRLTKLSEPVNFIYNTANWM
                     APWRLETPDSERVRLIFTPFFERVAKTDALILTSEVHQMIGRFDGTLLTADGETVAVS
                     QAVGWAEDHNARW"
     gene            complement(13994..14818)
                     /locus_tag="PKL11_03965"
     CDS             complement(13994..14818)
                     /locus_tag="PKL11_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12790.1"
                     /translation="MSDFFGQPVGVLQNQLLRLEYLLSGGPRLVRLFYRHHPENVLVE
                     VPDIAIPTPAGDYHLWGGHRLWHAPEVMPTTYAPDDAGLEVVPLSDGVRLIGPIEPQT
                     GIRKQLEVHLHADQPIVELRHTLKNCGTAPVELAPWSLTMLPLGGEAHLPFSAPQAAS
                     LLPNRSVIYWPYTQLNDPRLCWEADALCLVAQPSETPLKLGWFNHLGWITYTRAGIAF
                     TKRFTPQPDLPHLDNGCNAEIYAYNRFIELETLGPRIELLPDQDVEHLETWELTDA"
     gene            complement(14818..15441)
                     /locus_tag="PKL11_03970"
     CDS             complement(14818..15441)
                     /locus_tag="PKL11_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ12791.1"
                     /translation="MPTLLLIRHAANDVMNARLAGRLPGVHLNALGRQQAADLARALA
                     ALPLSAVYASPLERAVETAAPLAAARQLTVQVEPGLNEVDYGSWQGCTYKSLRRRRLW
                     QTLLADPASMRFPDGGTLIEAQQLAITTLEQIATHLPKNEFVACITHGDIIRLSVAHY
                     LNMPMLEYHRLCISPASVTVLRLNESQPRVVRVNQSFSTNPDEEFFK"
     gene            complement(15404..16810)
                     /locus_tag="PKL11_03975"
     CDS             complement(15404..16810)
                     /locus_tag="PKL11_03975"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ12792.1"
                     /translation="MDLFDHAMQSQMDQEAPLAARLRPRNFDEFIGQEHIVGAGRLLR
                     RAIQADRLFSSIILTGPPGTGKTTLAQLIAQHTQAHFESISAVLAGVADLRRVIVEAQ
                     ERRRLQRKRTILFIDEVHRWNKAQQDALLPHVESGMVTLIGATTENPYFDVIPALVSR
                     SRVFELKPLTDEQLSTVLDRALTDPERGYGRLTVHLDDDARAHFLRLCGGDARNLLNA
                     LELAVETTQPTLAGEIHVTLAVAEESIQRRAVVYGKNGDPHYDTISAFIKSVRGSDPD
                     AALYWLAKMLHAGEDPRFILRRLIILAGEDIGLAEPRGLLVAVAAAQAFEYIGFPEGV
                     FPIVEATLFLATAPKSNSTTGYFEALKRIEQSGAGPIPLPLMDASRDAAGLGHGKGYQ
                     YPHSFSGHFIPQQYLPTEVLGTHFYQPSDQGEESAAAARVAAWRAAQAAALAGDPPPA
                     PGEKDSDAHPAPDSPRSQ"
     gene            16997..17191
                     /locus_tag="PKL11_03980"
     CDS             16997..17191
                     /locus_tag="PKL11_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNZ12793.1"
                     /translation="MKAFVDPDICIGCGVCETIAPNVFQLSDGGFAVVIVDEVPVGTE
                     GDVRQAAEECPEGAISIFED"
     gene            17248..17493
                     /locus_tag="PKL11_03985"
     CDS             17248..17493
                     /locus_tag="PKL11_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12794.1"
                     /translation="MDILLLDGSLRVKIYYDEDDSTFEDNICICLEEDCPEEERILRA
                     DEVRISLTPLEARRLARALDAAADTSLANYETSPQED"
     gene            17500..17919
                     /locus_tag="PKL11_03990"
     CDS             17500..17919
                     /locus_tag="PKL11_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNZ12795.1"
                     /translation="MDIFLSIKFYADQRNRAWVEALTAALAEHGLKVQCVVRDIENWG
                     MVSLSAAELMRQTFDLLERCSLVVVNLSDKGVGLGIEAGYAYAHNIPVMAAVPQEADL
                     STTLSGISRWTIRYAGMEDLTRQILNAVDELALQEVQ"
     gene            17916..18413
                     /locus_tag="PKL11_03995"
     CDS             17916..18413
                     /locus_tag="PKL11_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12796.1"
                     /translation="MNDSEKTRLAIATGAMGILFPVVGLILGWSIKGFKTGLLIAGVL
                     FGVFFLFSLLFLAQVRNPSKFIVALPFLMGALYNILPDALPLGLDDAAAGVLGGLLTF
                     LLWLRRQPNTPRWIVIPLVLAGLYALVGGFIPGPLDELLVFGAAGLTAWLGGRTPGLP
                     PPQNL"
     gene            18410..19228
                     /locus_tag="PKL11_04000"
     CDS             18410..19228
                     /locus_tag="PKL11_04000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12797.1"
                     /translation="MIPTILHDRAAIAAYLRQDLLVHLYELGDLDDFFWPYTTWYASL
                     NHGDIERLALLYTGSELPILLAAAAADDQPMINLLKALLPFLPRQFYAHLPAGMDFGF
                     LRAEYQMESYGVHDKMALQERTGAGSLPHPADKIRQVKIEDLPELIGFYEAAYPGNWF
                     DPRMIETGQYYGLWRSGRLVSVAGVHVYSPAQGVAALGNITTLPELRGSGLGTAVTGA
                     LCQALGNQVGTIGLNVRADNTAAIACYQKLGFRRVTQYEEWMLKTRPLVEDRDD"
     gene            19221..19802
                     /locus_tag="PKL11_04005"
     CDS             19221..19802
                     /locus_tag="PKL11_04005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ12798.1"
                     /translation="MTELWLVRHGQTDWNLEKRYQGQADIPLNLTGLQQAADLANRLE
                     GEAFEALYSSDLSRARQTAELLARTLGLPLRLDARLREIHQGEWQGEYLDDVRERHTL
                     LLEEHWNNPARSRAPGGESVIEVAGRAAQAISEICRRHPRGRVLVVSHGLTLATLICQ
                     ARSASLARVYSLIPENTQVEVIYWPPPRLLQEN"
BASE COUNT         4540 a         5186 c         6270 g         3950 t
ORIGIN      
        1 cggattgcga gcgtggcatt atttttcaaa gcatcgggga tggtgtaggt ggtctgaaaa
       61 acaccgcctg tgcccgagtt cgtggtagca accacataac caccaatggc gtaggtgccg
      121 tactcgccga tgcgaacggt gaaatccatg ttggcgggga agtttttagt ttcaatagtg
      181 acagagatgc ccgggactac gctgacgatc ttgaaggtgg gaatgggaac agatgccgca
      241 ttaacgggtg cggcaggcat ggcagccgtc aacagcagaa cgatcaacag agcaatgacg
      301 accccttgac gaatattacc agaaaatcga aacatcttca gcctccttgc acaggtgaaa
      361 caagagtcct gtatccatta ctatagcata aaaccggcgg caagttacac ggcagaaagg
      421 tgaattctgg caggggaaag cgttcagagc tgttttttgc gggcgcagat ggctgtcttg
      481 agacgccggg cgcggacaag cagcgtgatg atgtagatca ccaggagccc cagcataaca
      541 atataaccac cgaccatcca gggaagtgaa ttcatatcgg gctccttaat ccatttgctg
      601 acgcagcgcg tcgagagtgt ctgcaaggcg cgccaggcgt acgcggcggt aaagcagagc
      661 cgccagcaat accgaaaagg ctgccaggct gatgagcagg gtttgcgtca tttgaggggc
      721 aagctgcatt ccatcattgg cagtgctgcc cagaagcagt gggtgaatgg tgcggtagag
      781 gtgaatggag aggtacatga gggggaccga taaaaagccg accaccgcat agacggcccc
      841 aaagcgcgcc cggcgttctg gatcatccac tccctgccgg agcagaaaat aggccagata
      901 gaccagttcc atgatggtca ccgtcgtcag gcgcgggtcc caggtccacc aggtattcca
      961 ggcctggcgg gcccagatgg agccgcccac aatcgccatc agcatcagca ccaggcccac
     1021 ctcaaccgca gaaaggctca gttgatccca gcgtgtctgg cgccgggcca ggtagatgcc
     1081 tcccgcgatt gccgcaatca taaaactgag catcccagcc caggctgcgg aaaggtgaaa
     1141 gtagaagatc ttttgcacag cgcccatggt ggcttccaga ggagcgtaaa aaatgaccac
     1201 tgccagggcg cccaggacag ccagggctgc ggcaatatcc agaatgcgct gcgtgcgctg
     1261 accggggtga atttcagatt gttccatcat gcaatgccat cctccaaggg attgggatta
     1321 ttcttccatc acgatatcaa aggccagcca ggcgacaacc gtaaaaatca aatcaaaagc
     1381 cagcaagagc atcagccagg gctgaacatc aacccagggc aagcccgata acagggcgct
     1441 actggccttg accgcggcga tgagcgcggg gagggttacc ggaaagagca agaccggcag
     1501 caatagatct cgagagcgag cctggacagc catggcagcc agcagcgtgc ccacagccag
     1561 atacccttca acacccaggg cgataaacaa taacaggccg gggagaatga gattttggtt
     1621 gtagagcaaa ccataaatcg gcagcagcac ggctgcaatc agcatcagga tcagccaacc
     1681 cgaaaaaagt ttgccaaaat aaagggctgc acgatccact ggcgccagca gcaggccatc
     1741 cagacagccg cggtcttttt cagagaccca ggcacggttg aggcccaacg ctccggcaaa
     1801 acagagtgtt acccagagga tgcccgctga tatttctgcc cgggcggcgg gtttgagttc
     1861 cagcgcaaag ttgaatatga agatgacaag cagggcgaag accagcatgg ccgaaaaggc
     1921 ttcacggctg cgccattcgg tcagcaaatc cttccaggcg acggtgaggg cggcgcgcca
     1981 gaagcgggga cggcggttca ttgggcggcc tccagtgact ggcggtagta cgtcaagaga
     2041 ccatcggggg gcagggccgc cgtagcggtg gaagcaatga tttggcccct gaccagaata
     2101 tccacccggg aggccagcgc ggccgagcgc gccagatcat gcgaggccat cagcagggta
     2161 cggccctgtc cggccacctg actgagcgct tggtcaagca tgacgcaggc atcctgatcg
     2221 aggccggtat ggggttcatc cagcagcagc acctgaggat cggggagcat ggcgcggcca
     2281 atagcaaggc gctgctgcat tccacgcgaa aaggtgcgca ccaggtcatt gcggacagcc
     2341 tccagaccag tgagcgccag cagggcctca attctggcgg cgagatgagc caccccgtag
     2401 agctgaccgt agaagcggag attctcagcg gcggtcagat cgccgtaaag caatggctgg
     2461 tggctgacaa cccccaggcg ggagcgcaga tccatggcct gatgcggcag cagacagccg
     2521 gcaaccgtga cgacgccgct ggtggggcgc agcagccccg cgaagacccg caaaagggta
     2581 gtcttgccgg caccattcgg gcctagcagg gccacaaatt cccccggggc agcggtaaag
     2641 ttcaggtcgc ggagaatggt gcggtagcca tagcggcgca ccaggttgcg cgcttcaatc
     2701 atgattgatt gacgactcgt tttcggcgga atctgccgcg cgtaattgct ggcgcagtgt
     2761 ggtgcggcgc tgatggtagg ctgagtttga gattttgccc gcccgcgcct gatcgtccag
     2821 cgcgataatg gcatccagaa tggcttcggg ggtttgcggc ggatcttcca gggccgcgag
     2881 agggcggcgc gtagcgcgca gccaggcaat tgtgatcagt aaggcgataa tcaacatcag
     2941 gctgccgatg aggagattga tccaagaatt ggactggatt ttcatgcccg aacgctcgcg
     3001 caggctgaag gagagatcac tggacagggg caggtcgtac gcgcggtaaa gttgtagttg
     3061 ggcgccttct acaacccgtt caccggcatc ttcaagctgg tcgctctcca ggctgaagac
     3121 ctctttgggc agcataatca aggccatgga aaccggcact ggggggtgaa tattgatgac
     3181 atcgcccgat ttgaagggca gatcgtaagc aaaaacaatc tgatagttgg ttcccggcgg
     3241 gatggcctga tagtcgccaa aatcagcgcc aaggatgctg aaccgcccgg cggtgatctg
     3301 gtcggcgaat tgcaggtttt cagcctggtc aggcaggtcg aaccacaaaa ccgggtcgcc
     3361 cggctgctcg gctaccacca ggcgattggt tgcattgacc aacaggtaca tttgtacaac
     3421 ctgaacccaa cctgagcgcg aataatccac cacaacctga aggcgctcgg cggagagggc
     3481 cgagcgatca gtggtggttt cgtagatcgt tagcggaaga ttgacatccg cacttgaggc
     3541 aatgccattg cctgaaagcg ggtcagactg gaaggtgtag ttgctgtaaa taacggagac
     3601 ctgataaatc caatcaggat ggaggggaac attatcaaac cgataggtcc ctccgagaag
     3661 atcattcaac ggctgggcag cagaatatac gttttgtgag ccctggtaag cgtttaaggc
     3721 aatttccaga tcggcgggaa gaccgttttg cgatccattg gttaccgagc cttcaatatc
     3781 cagggtagcc attggggtgg cggccgggct gacggagaca attgacagcg actgaggcca
     3841 gccaaagagg aaggattgaa catagcccgc aaccgagcgc aattcgtatt cgctgaggcg
     3901 attggcaaag gctggcatgg acggtgctaa tccttgagcg atggattctt tcagttccat
     3961 ggcactgtag gacgacaggt tggcaggaat ggtccaattg ggcggggtga ggccagattt
     4021 gagtgccttg tcgccatcgc cagcgccgga ggtgccgtga caggtctcgc agcggcgttc
     4081 ataaatggtt tctcccaggg ccagactggc ggcatcggtg ttttgcatga agaggtaggc
     4141 taccaggtcc cagcgctggc gctccgtcag cgaggtacca tacgacggca tctctccaga
     4201 gggtataccg ctggtaatcg cgtcaaacca atctgccggg cggctggtgc gggcaacaga
     4261 catgtgattg aaggggcgca ggggctgatc gagtgcaacg acctggggac ccagactttc
     4321 gtttgcctgg ccgtggcaat cctggcagag atgggcatac aactggccac cgttggtggc
     4381 gtctggcagc aggtaggggc cgctggcaac agcggtttga gatggggatg ctgaaggctg
     4441 ggggtcaaca attagcggca tgggcgttgg ggtacaggct gataagaggg caggaatcaa
     4501 taaagcaacc atcacccccc accaactgtt tttgcaaacg ccatcagatg ccagtttcaa
     4561 accattactc cgttatgaca gggttttacc gcaccgggca caaaaacggt ccgactgctg
     4621 caccgggcgg ccgcattgcg ggcagaaacc cgcggccgtt tcctggcggg cggcgcggcg
     4681 cgcagccaaa cgctccgtaa gggcgctgtc ttctgtggga gagaacagtg aggtgtcttg
     4741 aggtggcagg gtgctttcaa cggtctccag gcgctgcaaa acggacgcgc cggtgaccag
     4801 caagtgattg cgctgggtgg tgtactcatt cggggcaatt tttcccaggg cagagtccga
     4861 atccagttct tgaatcgcgg caagaattcg ctcgcgctcg gcgatgagat tgcggcggat
     4921 gacaatatca ggatgaagtg cggtatcaac gaccagggct tcaccagcct ggggctggaa
     4981 gaaggggcga ataatccaga tacctgccag cagggcaatg gcgataatga gaaataaaga
     5041 gccgagatcc attatggcgg tctcctaggg cgcgtaagcg ctgccgacaa actgctgaat
     5101 ttctgccaga gaggtaaagg ggccaatctt gacaccccgc acaactccgg aagcgtcaat
     5161 tagataggtt tcgggcacgc cggtgatgcg gtaggcgcgc gagatttgcg taccgaggtc
     5221 ggggccgttg ggataggtga tgtcgtactc gttgaggtag gccagggctt caggttcagt
     5281 atccacataa tccacgccga taaagatgac attgccagtg ggctgatatt ctcgccaggc
     5341 ctgttcaaga tcaacggctt cttcggcgca ggggctgcac caggaagccc agaaattaat
     5401 cagaatgact tttccggcaa ggtctgtgct gctgatcgtc tgaccatcaa aggtggtgag
     5461 agtaaactca ggagccgtgt cgccgacttc gggtgggccg gactgggctc gagacaggct
     5521 gatgcccagc aggacaagaa acccgaccag caggataaag gccacaatga cagcccaact
     5581 gatgcggcgc ggcgatgttt ttgagggcag agcagcagac attattcatc ctccattgag
     5641 gcatccgctt tacgctgctg aagttccgct tcgagacggg cgatggcttc gtcagacacg
     5701 atgggggcag gctggttcac cggggcgcgg ggggcaaacc ggcgaagcac caggaagaga
     5761 ccaattagaa aggccagcgg cggcagcaca taggccagga tgttcaggcc gcggggcggc
     5821 ggggcgccga ccacctgatc gccatactgg gtgagaaaat attcacggat ctggtcttcg
     5881 ttccaaccgt cggcaagctt ctgccgaatc agatcgcgcc actgggcgca agcctgggtg
     5941 ccgcaaacat ccaggggaac attctcgcag accgggcagt agagctggct ggcaatggca
     6001 ttgaccgcat catctgaagg cgtgggctgc tgggccagag ccgcaggcgg gggcagcaaa
     6061 aatgccagcg cgagcaatcc ggcagtcaaa gccagggaga tgagtccggt gtaagctgta
     6121 tgcttcatgc aacctccgca aggggcgtgc tcttacgcgg cggccaggca gccaccagcg
     6181 tgcccagcat aaagatcaaa ccgccaatcc acaaccagtt gattaatgga ttgtaatata
     6241 ttttaaaagt cgcctgattg gtgtaaatcg ggttccagtc ggccaaaatg atatagaggt
     6301 cgccagccag cgtgctgtaa acaccgggca ccgtcatggt ttgctgggcg tcatagaagt
     6361 aatcgctgcg gggggtgctc tgtttaatga agcgagctcc tcgattgacg gtcagattgg
     6421 cggcaatgac attacgccca tcgtttgaat cccaggagga aattgattgg agggttacgg
     6481 tataacccgc cagcgagacg ctctgcccgt tctgcagggt ggcttgcgtc tgagtctgat
     6541 acagttcaat gccaatgatg ccaacagcca tcagtgcaac gccaaggtga atgatgaacc
     6601 ccccatagcg gcggcggttg aggttggtca gccgccagaa tgcgtgcggg agggcttctt
     6661 tcaggcgtag ggagcggccg cgtacagccc ggctatattc caatacagtg gcgatgatca
     6721 ccagagcagc aaagcccagg ccaaccagtg cggccacctg gcggacgcca aagagcagta
     6781 agacaatcac aaccacaacg gatccaagcg cgggccagag caggcgcttg cccagagaac
     6841 gggacgaacc tgcgccccag gtggtcagtg gggcgatgcc tttcaggagt aacagcaggg
     6901 caaacaacgg cccggcaaca ctttcgtaat atccggggcc catggtgacc tgctgtccgg
     6961 tgaaaatttc tgaaatcagg ggatagatga cgccccacag acaggcaatc aacaggccca
     7021 tcatgacgag attgttcatc aggaagaggc cctcacggga gaaggggctc tgaagctggg
     7081 tatcggcgcc aaggttgcgc cagcgcagtg ccagcaaaac gagcgagatc agcaacgtca
     7141 gagcaataaa gccgaaaaag agcgggccga tggcgctttg agagaaggca tgcaccgagg
     7201 agaacacccc tgagcgggtg agaaaagtgc caaaaatgac cagcgaatag gttagaatca
     7261 tgaggatcat gttccaacgt ttggaaagac cgcgcttctc ctgcatcatg atggagtgga
     7321 gaaaggcggt gccgctgatc cacggcaata gggcggcaac ttccacgggg tcccaacccc
     7381 agtagccgcc ccagcccagc acatcatacg cccaacgact gccgagcaaa agccccagcg
     7441 aaaggaagag ccaggctacc agcgaccagc gccgggtggc tcgaatccat tgatcatctg
     7501 agcggttggt gatgagcgcc gcaatggcat aggcataggg gatgatgaag ccaacaaagc
     7561 ccagatagag cgccggggga tgaaaaacca tgcccggatg gcgcagcaag ggattcatgc
     7621 cgcggccatc gaggggaatg agcggtaaag cccccgccgg ttggaacagg gcaaccatct
     7681 cgctgccgcc cgccgtgcgc cagaaacgca caaaggggtt ttcgacaaaa atgaccaggc
     7741 tgaggaagaa gattagcgtg atggcgatga ccaggatgat ccacggcaac aggtcttgat
     7801 cagagtgcca ggagcgcagc gcgacgagaa aggcagacag gcctaacagc cagcaccaaa
     7861 agagcagtga gccggactgc ccgccccaga tggcggtcag tttgagatag aagggcattg
     7921 aattgctggc tacattgtag acgtattgaa cctgatagtc ccccgcgaca atcagcccgg
     7981 caagcgccag catcgagagc gataacaacg gaaaggtgag cacggctgcc gcacgggcgc
     8041 tttcggccca gacgcgggca ttacgggtaa cgccaatcat ggcggcaatg ccgctgtaga
     8101 tcgcaacgat cagggtgatg atgagcaggc caaagccgag attggaaatg agcatggcgg
     8161 ggtcctatgg aacagccgtc tcagcttgct gggggattga ttcttcgtag cgggtgggac
     8221 atttgagcag taattcttcc gcttcaaaga cgccatcggc gcggagtgtg ccggtgacaa
     8281 tggcctgcgc ttcgtttttt aattggtcgg gccggacgcc agaatattca attttcaatc
     8341 gagttcgagc ggggtcttgc acagctgcat gcagcacagc cgccaaacca ccctgtgact
     8401 caatctcgtc attatcgccg gggatggaga caatggtaaa gctgagggtc agcgtgttgg
     8461 ggtcgtagta gatagtgtca ccgaggactg cgcctgagat gcgcacactg cgaccctgca
     8521 tctgctggga ttgctgcagc agttcatcca cggtcatgaa atactgggaa tttttttggg
     8581 ttgcagacac tattagaaag accattgcag ccaggatcag caggccgccg atgatgtatt
     8641 tagcacgcat gaaatcattc ctccgggaaa tgagcggaat ccgctctttg gtcattttct
     8701 cacactggga ttaactgaga ctgaaaactt tttaagttag atgtgagaat caattaaggt
     8761 ttgggaaata ggcgataatt atactaattt tgtctttttt gcacgggatg agccgttatt
     8821 tcggttgagt taatttactg agcacagggt gaatatccca ggggatgtga ttgctcgtca
     8881 gcagttcaat gaggctgctg cgttcaagaa tgcggcggcg ggtcaggtct tcggggccac
     8941 gctgtgccag actccagagc aggtcggcga catccagggc ggcacggggg cgccccagca
     9001 cggcggcgct gcgggtgcgg gcatccaaca gggcatgttg attgcccagc caatgtgaca
     9061 gggtttgcag tacttctaac ggggattgga cctgctcccc tgctccattc tcaactacat
     9121 aggcggcatt gcctgtttcc tgcccgggga tggcatcaac cagcagcaga ggcaggccag
     9181 ccgccaggga ttcagtcaca atcaggccgc cagctttgca aagaatgcaa tcggctgcca
     9241 acatcatttc ggggatgttg gaggcaaatt cataaatgtg agcggggaca tgccattcca
     9301 tgctgcggag ttcggcaaaa agctcagcgt ctttaccggc aaccgcgatt atctggagcg
     9361 gggtgccgaa atgattgacg acattcaggg catctaccag gcggtccacc cggcgggagc
     9421 caaccgccag caccgtcagc aggtcgggct gccagccgag gcgggtgcgc agctcagctt
     9481 tgggcaggcg gggtgcggca aataacgggt tgaccgggat gcccagcggg tgaatttgat
     9541 gtggcgccag gccgtaattc aacgccaggt tctgaacaat gggggttggc accacgcaag
     9601 catctacgcg tgagacaaac caggtgcggt gcacggtggc aaggtcagtg actgtggtga
     9661 caagggggat gtggcggcgg ttgatggcaa agacagagag gagtgggatt tgatagagtg
     9721 ggtaagtggt caggatggca tccggctggt gggtatccac caggtcacgc ataacttcgt
     9781 agagcagcag cgtgagcgta ctttccataa tcgccgaggg aaccatataa tcactggcat
     9841 cgtaccccaa ctgatagagc tgaggcgaca ggcggacaat acggtcataa tctgcagcgc
     9901 tctctcgcag gaggggcgac acacgggggt cgtcaatggg attgatgatc gtcacctcac
     9961 agcggtcacc atactgctgc gcgagggcag ccgcaaccgc attggcggcg ctgcgatgtc
    10021 caaagccggc atcggcggtt aagatcaaaa ggcgtttggg cgtatccatg gatcaaccct
    10081 ccaataaaag atggctcatt ccgggcgggg ctggccctgc aaaacttcca gcagcagttg
    10141 cagggccaga tcggcgctga aagatttatt ctcctgacgg ttgccctggc ctataaaatg
    10201 ccaggcaaag tcaccggcag gggcactaat gccaatccat accaggccga cgggcttatc
    10261 tggcgtggcg ccgccggggc cggcaatccc gctgacggag aggccaatga cctcatccag
    10321 ggggcactca ccacccagcg attggcgcac accgcgagcc atttccagga cggtttggcg
    10381 gctgacggcg ccgtgggcca gcagtgtttc ggggcgaacg cccagcagac gctgtttgac
    10441 gccgtaagaa taggcggtga tgctgcccag gtaatactct gaggagccgg gtacgttggt
    10501 gatccggtga ccgattaaac cacccgtgca ggattcagcc acaaccagct taaggctgca
    10561 ctggcgcagc caatcgccaa cctgaatttc caaagattgg atcaaatgaa acctcgcggt
    10621 taaagaatag gttgattata caggatgata ccgccgatcc aggggagaat atcccagccc
    10681 cagcagtttc tcaggtacaa ttatatgtgt aatgaaaccc caaaagctga tcctggtttt
    10741 gcttgttgtg ctggcgctga ccatgcggtt aatccccgga ccgcggacga ttgacgactc
    10801 ttacatcacg tttcggtatg cgcgcaacct gctggctggt gaagggtttg tctataaccc
    10861 gggtgtgccg gttcagggaa ctaccacgcc gctctacacc ctgctgatga ccggactggg
    10921 ggccctggcg ggcggaacac aggctgattt tccggtgatt gcgtggttgt tcaatgcggc
    10981 agccgatgct gccgcctgta ttctgctgtg gttaatcggt aaaagggtgc ggcatgaact
    11041 ggcaggattg ggcgcggcac tggtctggag tgtggcgccc tacagcgtca cgttctcgat
    11101 tggcgggctg gaaaccagcc tgtatgtgct gctgctcacc gggctggcct atgcttacat
    11161 ctcgcaaaag cgggttttga ccggtttgct gggggcactg gcgctgctca cacggccaga
    11221 tgcgctgatt tttttggggc ccttgatgct ggatcgcttc atccgggctg tacggcccgg
    11281 gctgctgggc gtcaaaactg agcctgagcg cttgcagttg ggcgaagtgc tggcttttgt
    11341 gatcccggta gcggcctggt atgggtttgc cacactgaca tttggcagcc ctctgcccca
    11401 ctcggtgcag gccaaactgc tggcctatca tctggaagca cacgaggctc tgatccggtt
    11461 aattcaacat tatgccacac catttatcga gcaaagtacg ctggggacaa agattgccgt
    11521 agggctggga ttggtgcttt atcccttctt ttacattctg ggcgcccggc gagtttttgc
    11581 ggctgagcgg cggctgttgg cctggatgct gttcccgtgg ttgtacttca tcacgtttgc
    11641 aattcctaac ccgttgattt tccgctggta tctgacccca ccgctgccag cttacttttt
    11701 ctttgttttg gcaggagccg ggagtttgtt caagttgaat ggggatttac gcacagccct
    11761 ccggcgcagc tggatcgcgg cgctgatcat tctgctgctg ccggttggcc tttcactgcg
    11821 cgcctggcaa ctgcatccgg accatgggcc agatcgcccg gcgcccggaa tggcgtggtt
    11881 tcaattagag ctgctgtacc gagaggcggc tgagtttgtg gcgccccaga tgaacgccga
    11941 taccgtgctg gcggctggag atgtgggggt gctgggctac tacactcctg cccggattct
    12001 ggacacggtg ggtttgaact ctgaagaagc gctgagctat tacccgctgg acgaggccta
    12061 ttatgtcatc aattatgcgg ttgcgccgca gttgattctg gatgaacagc cggactgggt
    12121 gattattctg gaagtttatg ggcgcgaggg gctgctgaaa aacgaaactt tccagcaaca
    12181 ttatacgctg gtgaaaaagc tggaaaccga tatttacggc agcgatggga tgttgatttt
    12241 tcagcgggcg cgttaaagcg cacaactgaa tcgtcaacac ccgattgatt atcccaggtt
    12301 acgagccaaa ggcctcttcg taatcggcga tggcggcctc aacaacggta taagccgcct
    12361 ggcggtcgta agtaccggca ataaaagtca cattctcttt acccggcgtc agcttgtaag
    12421 tgccgaaata atggcgcagc cgttccacga ggacgggggg caggttttca atatcctgtg
    12481 cgctgtccca gaagctgtcg tttgccagca cggcgataat tttgtcgtct gcctcgccgt
    12541 tatcaatcat ctgaatgccg ccaatcacgc gagcgttcag caccacttca gacttggtga
    12601 tggggcgctc tgagatgacg caaatatcca gcgggtcacc atcgccgcgc tgcgattgcg
    12661 gagacaggcg ctgtacccgc acgccgcaat aggtgcgagg gatgatgcca tataatgtag
    12721 ggggctggga ggaactgcgc tggggacgat ctacccgcag ataccccgtt tctttatcaa
    12781 cctcgtattt gacgaggtca aagggggtaa tttcgatata ggcatgaacg atttccgggg
    12841 gcttagggcc gatttctagc ccgtgccagg ggtgcggacg ccagcgatag aatggatgcg
    12901 gaaaagaaga catgggatac ctcccgccaa gaatcgggtg tattataccg ctttatccta
    12961 ccagcgggcg ttgtgatctt cagcccagcc aaccgcctgt gaaacggcaa ccgtttcgcc
    13021 atctgcagtc agcagggtgc catcaaagcg accaatcatt tgatggactt cagaggtcag
    13081 gatgagggca tcggttttgg ccacgcgttc aaagaagggg gtgaagatca ggcgcacccg
    13141 ctcgctgtcg ggggtttcga gtcgccaggg ggccatccaa ttagctgtgt tgtaaatgaa
    13201 attgacaggt tcgctgagtt tggtcagacg accatccacc aggatgccgt tttcggtcat
    13261 gccggtgtca tccgtccatt tggcgcccag gttgacgcca atctgccgcc cgttctggca
    13321 ggttgagaag gaagcccagt tccatttgga ggcgtagggc cagatgccgc ggccataatc
    13381 aagacaggca aaggcagcat caggggaaaa cttatattcc tgcgagtcga aggtcaggct
    13441 gccagaaaca ggcaggcaat tctgcttgga ggtgaactga aaatgatctg catcccaggg
    13501 gataacgaca ttgagcgtct catgtccggc ggggcggcag gcgttcaggg cagtggtgag
    13561 cattttgcca cctaaatcct gactgaggac ctcaatatcg atctggttgc cagcatccat
    13621 gaaggcgatt tgcaatttag ggttctggta gtgaatgcgc ccggtcaccg tgggaggcaa
    13681 ttcaaagcct ttgccaagcg gaatggagac tgtttgttcg gcgaatttga gggtatgaaa
    13741 atcgaggaaa tagatgaacg ccattcccag ataatcaata ttggagacgg tagccgaaaa
    13801 aagacagtcg ggactggtga tgcaccagta attccaacgc tttttacgcg gccagtgccc
    13861 gctgacgttg cagttatgca gcggctggcg agaccagccc accgcggcgc gattaagccg
    13921 cccggcagag tcacacaggt caaccggggc ggtcagttcg ggttcggggt gcagggtatt
    13981 gcgaaaagac agatcaggca tcggtaagct cccaggtttc aaggtgttca acatcctggt
    14041 cgggcagcag ttcaatgcgc gggcccaggg tctccaattc aatgaagcgg ttataggcat
    14101 agatctccgc attgcagcca ttatccaggt gcggcaggtc tggctgaggc gtgaagcgtt
    14161 tggtaaaggc aatgccagcg cgggtatatg tgatccagcc cagatggttg aaccagccaa
    14221 gcttgagcgg cgtttcggaa ggttgggcaa ccaggcacag cgcatccgct tcccagcaga
    14281 ggcggggatc attgagttgg gtgtaaggcc agtaaataac gctccgattg ggcagcagcg
    14341 aggcagcctg cggggcgctg aagggcagat gcgcttcgcc gccaagcggc agcatggtga
    14401 gcgaccaggg cgccagttcc accggagccg tgccgcagtt cttcagggtg tgccgcagtt
    14461 ctacaatcgg ttggtcagcg tgaagatgga cctccaactg tttgcggatg ccggtctggg
    14521 gttcaatggg gccgatgagc ctgacgccat cagaaagcgg cacaacttcc aacccggcat
    14581 catcgggggc ataggtggtg ggcatgacct ctggcgcgtg ccagagccgg tgcccgcccc
    14641 agaggtgata atcccccgcg ggtgtaggga tggcaatatc gggaacttca accaggacgt
    14701 tctcgggatg atggcggtaa aaaaggcgca ccaggcgcgg gccgccagag agcagatatt
    14761 cgaggcgtag aagctgattc tgcaagacac caaccggttg accaaaaaag tcgctcatta
    14821 tttgaaaaat tcctcatcgg ggtttgtgga gaaagattga ttgacccgta caacgcgggg
    14881 ctgactttca ttcaggcgca gaacggtgac cgaggccggg ctgatgcaca gccggtgata
    14941 ctcgagcatc ggcatgttga gataatgagc cacagacagg cggatgatat caccgtgggt
    15001 gatgcaggcc acaaattcgt ttttgggcag gtgggtggca atctgctcca atgtggtaat
    15061 ggcgagctgc tgagcttcaa taagcgtgcc gccatcgggg aagcgcatgg acgccggatc
    15121 ggccagcaag gtttgccaga ggcggcggcg gcgcagggat ttgtaggtgc agccctgcca
    15181 actgccgtaa tccacctcgt tcagaccggg ttcaacctgg acagtgagct gccgggcagc
    15241 ggcaagcggt gcggcagttt caacggcgcg ttccagaggg ctggcatata ccgcgctgag
    15301 cggcagcgca gcaagggcac gggcaaggtc agccgcctgc tggcgcccca gggcgttgag
    15361 atgaacgccc gggagccgcc ccgcaagacg ggcattcatc acatcattgg ctgcgtggcg
    15421 aatcaggagc agggtgggca tcagagtctt tttctcccgg tgcgggcggg ggatcgccag
    15481 ccagggctgc ggcctgggct gcccgccagg cggcaacgcg cgccgcggca gcggattcct
    15541 cgccctggtc agagggttgg taaaaatggg ttccaagcac ttcggtaggc aggtattgct
    15601 gtgggataaa atgtccggag aagctgtgtg gatattgata gcccttgcca tgccccaatc
    15661 cggcagcatc acgactggca tccatcagag gcaggggaat ggggccggcg ccagactgct
    15721 cgatgcgttt gagggcttca aaataccccg tggtggaatt ggacttgggc gccgttgcca
    15781 gaaacagggt ggcttcgaca atggggaaaa cgccctccgg gaaaccaatg tattcaaaag
    15841 cctgggcggc ggcaactgcc accagcaatc cgcggggttc agccaggcca atatcttcac
    15901 ccgccaggat gatgaggcgg cgcaggatga agcggggatc ttcgccagcg tggagcattt
    15961 tggccagcca gtagagggcg gcatcggggt ctgaaccacg cacagacttg atgaaggcag
    16021 agatagtatc gtaatgcgga tcgccgttct tgccatagac caccgcccgg cgctggatgg
    16081 attcttcagc caccgccagg gtgacgtgaa tttcaccggc aagggtcggc tgggtggttt
    16141 ccacagccaa ttccagggca ttgagcaggt tgcgggcatc gccgccgcag aggcgcagga
    16201 aatgcgcccg ggcgtcgtca tccagatgaa ctgtcaaacg accatagccg cgttctggat
    16261 ccgtaagggc ccggtcaagc acagtgctca actgctcatc cgtcaggggt ttgagctcaa
    16321 aaacgcgcga gcgcgagacc agtgctggaa taacgtcaaa gtaggggttc tcggtggtgg
    16381 cgccgatcaa cgtgaccatg ccactctcaa catggggcag cagggcatct tgctgggctt
    16441 tgttccagcg gtgaacttca tctataaaaa ggatggtgcg cttgcgctgc aggcggcgcc
    16501 gctcttgagc ctcgacaatc acacggcgca ggtcggcaac acccgccagg acggcggaaa
    16561 tggactcaaa atgcgcctgg gtatgctggg caattaactg cgccaaggtg gttttacctg
    16621 tgccgggcgg gccggtaaga atgatggacg aaaacaggcg gtctgcctga atggcgcggc
    16681 gcagcaagcg cccggcgcca acaatgtgct cctgaccgat aaattcatca aagttacggg
    16741 ggcgcagccg ggcagccagc ggggcttcct gatccatttg agactgcatg gcgtggtcaa
    16801 agagatccat gcttctattc tatcacggct gggtggaggc ggcgggcaga agagcataag
    16861 cataaattct gcgcctggcg aacaggccaa accaacaaaa agaaatgtga gattaaattt
    16921 gtgggtgcca ttgacgaatt accgcgcttg catctataat gctaacatca ttattcccct
    16981 tcaaggagaa ggctgcgtga aagcttttgt agaccccgac atttgcatcg gctgcggcgt
    17041 gtgtgaaacg attgcgccta acgtcttcca actctcagat ggcgggtttg ccgttgtcat
    17101 tgtagatgaa gttcccgtcg gaactgaggg cgatgtccgc caggcggcag aggaatgccc
    17161 ggaaggcgcc atttccatct ttgaagatta ggttaaggtt ctccccaact ggattgggat
    17221 gaggcattaa tttttttgag gtgtgagatg gatatcttgc tgttggatgg ttcgctgcga
    17281 gttaaaatct actacgacga agatgacagc acctttgaag acaatatctg catctgtctc
    17341 gaagaagatt gcccggaaga agagcgcatc ttacgagctg atgaggttcg catctccctc
    17401 accccgctgg aagcgcgccg cctggctcgc gccctggatg cggcagcaga tacaagcctg
    17461 gctaactacg aaaccagccc acaagaagat tgataccggg tggatatttt tctttcaata
    17521 aaattttacg ctgatcaacg caaccgcgcc tgggtggagg cattaactgc tgcgctggca
    17581 gaacacggac tgaaagttca gtgcgtggtg cgggatattg aaaactgggg gatggtgagc
    17641 ctttcggcgg cagagttgat gcggcagacc tttgacctgc tggaacgctg cagcctggtg
    17701 gtggtgaatc tgtctgataa gggcgtgggc ctggggattg aagcgggcta cgcctatgcg
    17761 cataatattc cagttatggc cgcggtgccg caagaggcgg acctctccac caccctgagt
    17821 ggtatttcgc gctggaccat tcgctatgcc gggatggagg acctgacccg gcaaattctc
    17881 aatgccgttg atgagctggc tttgcaggaa gttcaatgaa cgacagcgaa aagacccgtc
    17941 tggcaattgc cacaggcgcg atggggatcc tcttcccggt ggtgggctta attttaggtt
    18001 ggagtataaa aggattcaaa accggcttgc tgattgccgg agttctgttt ggggtatttt
    18061 ttctgttcag cctgttgttt ttggcacagg tgcgtaaccc atccaaattt attgtggcgc
    18121 tgccgttttt gatgggcgcg ctctacaata ttctgcccga cgccctgccg ctgggcctgg
    18181 acgatgctgc cgcaggcgtt ctgggcggcc tgctgacgtt cctgctctgg ctgcgccgcc
    18241 aacccaacac gccccgctgg attgtgatcc cgctggtact ggcagggtta tacgcgctgg
    18301 taggcggttt tatccccggg ccgctcgatg agttgctggt gtttggcgcc gcggggctga
    18361 ccgcctggct gggtggccgc acccccggtc tgccgccgcc acaaaaccta tgattcccac
    18421 gattctgcac gatcgtgcgg ccattgcggc ctatttacga caagacctgc tggttcatct
    18481 gtatgaactg ggagatttag atgatttctt ctggccctat accacctggt atgccagtct
    18541 gaatcacggc gatattgagc gtttggcgct gctttataca ggctcagagc tgcccatttt
    18601 gctggctgcc gccgctgccg acgaccaacc gatgattaac ctgcttaagg cgctgctgcc
    18661 atttttgccg cgacagtttt atgcgcacct gccagccggg atggattttg ggttcctgcg
    18721 ggcagaatat cagatggaat cgtacggggt gcacgacaaa atggctctgc aagaaaggac
    18781 tggagcgggc agtctgccac accctgcgga taaaatccgg caggttaaga ttgaagatct
    18841 gccggaactg atcggctttt atgaggctgc ttaccccggc aactggtttg acccgcggat
    18901 gattgaaacc gggcagtatt atgggctctg gcggtctggc aggctggtga gtgtggcggg
    18961 ggtgcacgtc tattcgccag cgcagggcgt ggcagcgctg ggaaacatta ccaccctgcc
    19021 cgagctgcgc ggcagcgggc tggggacagc cgttaccggg gccttgtgcc aggcgctggg
    19081 caaccaggtg ggcacgattg gactgaacgt acgggctgat aacacagcgg cgattgcctg
    19141 ctatcaaaaa ctgggattca gacgggtgac gcaatacgag gaatggatgc taaagacacg
    19201 gccgctggtg gaggataggg atgactgaat tgtggctggt aaggcatggt cagactgatt
    19261 ggaacctgga aaagcgctac cagggacagg ccgacatccc gctgaatttg acggggttgc
    19321 agcaggcggc agacctggca aacagactgg aaggcgaagc gtttgaagcg ctttacagca
    19381 gtgatctttc acgggcgcgg caaactgccg aactgctggc ccgaacgctg gggctgcccc
    19441 tgcggctgga tgcccgactg cgcgagattc atcagggaga gtggcagggg gaatatctgg
    19501 acgatgtgcg cgagcgacac accctgttgt tggaagaaca ctggaataat cctgcccggt
    19561 cacgggcgcc gggtggagaa agtgtgattg aagttgccgg gcgagcagca caggcgatca
    19621 gtgagatttg ccgcagacac ccccgcgggc gcgtcttagt cgtcagccat gggctgacgc
    19681 tggcgaccct catttgtcag gcgcgcagtg catcgctggc gagggtatat tcgctgattc
    19741 ctgaaaacac gcaggtggag gtcatctact ggccgccgcc gcgcttgctg caagaaaatt
    19801 agcgctcgcg tccgcctttg agctggccct gaagggtttc cagttcagcc cactgacctt
    19861 cagccgccag tttggcctgc attggccagg tggcagggtc ggcaatccgc aggttggcga
    19921 ccgaaagaat ctcgcgcagg tgagcg
//
LOCUS       DAOPVS010000025        19774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2771_length_19774_cov_59.512551, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000025 DAOPVS010000000
VERSION     DAOPVS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19774
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_19774_cov_59.512551"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1007
                     /locus_tag="PKL11_04010"
     CDS             192..1007
                     /locus_tag="PKL11_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNZ12799.1"
                     /translation="MALKGNLRDFSITQLLNLINLARKTGLLVIEGSGEAARIHFRDG
                     KLAYAQYAVEDNGLAAILQRNGKISASQYQILKERAGHMTDKELGLMLVNAGYLSQND
                     IIESLQHYFIQVTRRLFTWADGFFNFDGENPPPDNRILVRVDLENLIIEGARQVKELE
                     QLQEEIPSLDMALKFTDRPGANIRSVNLSQDEWRIVSYVNPKNTLRQIAQAARLNDLE
                     IRRVVYGLLQAGLVELVRPGGAPLPPSGRMFPTSDRGEQRSLVNKLISRIRSL"
     gene            1030..1572
                     /locus_tag="PKL11_04015"
     CDS             1030..1572
                     /locus_tag="PKL11_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNZ12800.1"
                     /translation="MQTVKIVVTGPFNSGKTEFIQTVSEIDVVSTERRISSEAERVKE
                     TTTVAMDFGRITVDEDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFRE
                     ARSILETFRAYAPTPYVVAANKQDRPDAWELEDMRVALRLDSKVKLLPSVAVDRESVK
                     SVLLELLYSILAEMDSNPGR"
     gene            1593..2432
                     /locus_tag="PKL11_04020"
     CDS             1593..2432
                     /locus_tag="PKL11_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ12801.1"
                     /translation="MSSITTDQGILHYEVYGRGKPVILLHGWLGSWGLWQETMTFLGQ
                     YYRTYALDFWGFGESGRKLDTYLVNDFVALVDQFMDKLGIESAPLVGHSMGGTVSLLV
                     SLRYPQRVQKVTVVGSPIAGSSLAFLLKMAGIPFNASAIFTFFGLFRRIMRAAGPFIC
                     RDPRFMDMMDSDLTKLTLESFFSSIASLRRTDLRPELGNVQVPVLGMYGDRDNIVNPN
                     QWKLMPRIPRGTYLRFKNAGHFIMLDEPQPFKTQLKDFLDSPLPAPIITSSPNEVQLG
                     PSL"
     gene            2416..3036
                     /locus_tag="PKL11_04025"
     CDS             2416..3036
                     /locus_tag="PKL11_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNZ12802.1"
                     /translation="MAHRCNQSLTLAIIRGMKEVIGQTGVQAVLNLAQREQVLSARFN
                     LDDPRGLLFKDLAALHSILEVSYGPRGGRGVALRAGRAAFGHLLRQTGDQIGLTDMEY
                     RLLPTPQRIQTGLQKLAALTAASCAQPVEVTDTETAWVWRMSLCPECYRRQAAEPVCH
                     FVTGLLQEFMAWANSGRFHHVEEIECRATGGAACVFSIDRQPLELG"
     gene            3039..4316
                     /locus_tag="PKL11_04030"
     CDS             3039..4316
                     /locus_tag="PKL11_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12803.1"
                     /translation="MLKIILQCPRYLAPFNERARDLRIQNQPLWLHQRDLLAGITTNE
                     MELPPGARLPQVHEPCLVYRDNLFFDADFLNAFLAAARKRQTPSRAAISANDPAFREH
                     CLPLSVSYTPAGDMYLVDLWYYPDGPVPNAEPLVVDMQSRELGYYHVPTYMANETGDL
                     TYQIPLRSLIAIDSWVHIFIADCVFGLFGRGARVEKRLDEDLAFKLKVIGTSLYEGRQ
                     VLQCSSLVKIGRNCSIDPDAVIHGPTTIGDNVTIGAGAVIENCIIGDNVNVSQDCQLM
                     LSVVGDGTFLPFRAALFMTTIMEHGMVAQNTCLQMCVVGRNSFIGAGNTFTDFNLVGG
                     AIRARDANGELKPSNRPVLGGCVGHNCRLGSGLIFYPARMIESDVVMFASKERRVVDH
                     DIVYEESDHLRLKNPGIHPRLYPRPGEEVEETW"
     gene            4483..5373
                     /locus_tag="PKL11_04035"
     CDS             4483..5373
                     /locus_tag="PKL11_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12804.1"
                     /translation="MGKARLLIVEDDGDISNMLRIYFTSQGYDVDLAPRGSDALEKTR
                     QVMPHLIVLDIMLPDIDGYGVCRTLRMNTRTSHIPVIFLTQKDERSDRLQGLELGADD
                     YITKPFDIEELKLRVQNAIARAERESLTDPQSGLPAGRMIEDQLRRMIRQKGWAFMDI
                     RINHFDPFKDVYGFIAANDALRFTAMLIGEVADELGNDSDFVGHAGGDNFVLVTSEER
                     AAAIRQRLKERFAEAVLAHYSFMDRQQGYINVTDKNGQTTQAPLMTLAVGVVSPTTHS
                     FADIREITELAAEERRRDTQ"
     gene            5390..12331
                     /locus_tag="PKL11_04040"
     CDS             5390..12331
                     /locus_tag="PKL11_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ12805.1"
                     /translation="MFNNSEYVLAVLYILAALLLLGLVWILLRSARRLRPILQARPPV
                     DLPVTPAETHTDAVIVVQAGGRVMSINAAARQLFGLQPGESANLERLARRLRPSEPFI
                     NLCAAEGQARFVLEGKALQGVSYKLALEPEPVVVVSLRAPGEESRSGGSAALQKLQNA
                     PSFLAAMSADMDIDATILAILQSVDRLIDSDVVEMTIWDAGKEHLTPYRMVATPGAEP
                     RLDKPTQRYRMGEGFSGQLIREKQPLLITDTTTRPELRQAADPVSFPLRSYVGMPLLV
                     GSEFIGTLELGAVTRGNFQQEDLELLQLLAPQAAVAIHNTLLYNRERYRTAELAGLAG
                     LAQAFTTVSDPSSLFDRLLKSISPLIPVQILGFLLYNETTRRLEGQAPIIGLPEQFMD
                     LYNTEVAPDSPLEKALLDQDLIITGNAMEDPQWQVLGIDQLARGASLRDTALIPLNAS
                     GHTLGYLQASNHTDGGVTFTQDELHLLLIVANQAASIIENSRLVQQSRLRAQRAEALR
                     RITGLASSAATLDEIFQYSLQELARLLHADLGLAFVLDQNRKQLSLLQSAVIGELPDL
                     PERSRTLLVEDAQFAFTVTGAQSTINTPELTAETAIIPFYQQIFKAAGVQALLGVPLV
                     VRDEGVGELWFASRTPDSFNQADVQVLTTAAGQLAGVVEQAFLVAQTDASLRRRVEQM
                     TALTRISRELSTSLDLQSLLQLVYSEALRTTRADCGTITLFDLSRPEAETPRSRFSVG
                     DPVGRELSALELKVLAQAQPLNLPDVTAETGCIPSHEGIASAMIVPVIYRQRPAGLIT
                     LHGKTPARFDEDAVEIAKSLAAQAAIALGNAFQFEEESRRGELLRRQVDTIGKLFQVS
                     KVMRPGQNVEEALTAIANAISETSRFKFVVISSVDNAAGTMRRLANAGHPPEVWAEMR
                     TRRNPWNVVETLCQPEFRFGSCFFIPADRRPVVTQDVHFVSMLPAEETHEQDAWDTKD
                     LLFVPLYDAEGLPLGLVSVDGPVDGRRPDRPTLEALELLSMQVGVVLENSRLNQALQE
                     RTEDLQTRTDRLEAALSGAQQSLPLLLNRHLEQTITIQSLHRQNQRVQDGLALTEQVT
                     TQNDAPSLLRTLARQILTRFDLQTALIGERLGSELHIVEVIGAAPAGANPQALFGQKN
                     PLRQALQDGQPLLVSMLEREPEWKNIPLLNAFAAQSFIALPLPVTEDRSLALLAIGTD
                     SLPEFTTEDRQSYARLIRQVTISLQNLELLTETRRRLNEMNLLLDFSRKLGSLDPASI
                     LKALLENAREAIPVGTAGWVGLLNKEGQLEPLAASGYADSDALLEMRFPLEVSGRSLP
                     VSAFRANQPLRIDELNFVQEYTLSSDDLLRYRRATGGGLPIAALATPIGLGEHALGVL
                     VLENFTTPDAFIPEDEGVTQSLSQQAALALDNARLLQSAEQRAAQLQALTEVAGTITS
                     SLQTNDLIASLLDQLGAVIPYNTATLWLRSGSDVTVAAASGFADNESRLGLSARVEDS
                     LLFQSMIATAQPISVPDVREDVRFPSLLEPDHFSWLGIPLLAKGQVIGLIALEKTEPG
                     FYTPEHIQGATTFAGQAAVGLENARLFEESVRRAGELDQRSQRLALLNRLSSELGASL
                     DVDYILKITCQQLLSAMGANRVGAVMLGDGGRLTLQVEVPGAGERLPRQLPATPLFTR
                     LIESQGLFSTADAAAEADLLPIYETYLSQRHTRSLLIVPLMTGSTLHGWLLVQSVEVY
                     RYSLPELELARTISNQAAIAIQNARLFLETTRLKEDLERRVEERTAELSREHRNSQTL
                     LKISTELSASLDLDQVLVRTLSVLNESISAEQSLIVLTARPQKYSAGESLVDVAALTS
                     DSPEQQVAKWVVKRRSAAVIDDIATDARWQFPADHPFAYKSALAIPLTLGEDVLGALM
                     IFHRQGSFFILEQIGLLEAAVRQISIALNNAELFNLIRDQSENLGGMLREQQMEASRS
                     RSILEAVADGVVVTDASGAVTLFNPSAERILDLKADDVLKRPLSQFVGLFGHAAGAWF
                     ATIRTWSQNPATYQPGDIYSEQMDLDNGRVVSVNLAPVFWRSQLLGTVSTFRDITHEV
                     QVDRLKSEFVANVSHELRTPMTSIKGYVEIMLMGATGELNAQQKHFLQVVKGSTERLT
                     ILINDLLDLSRVETGRVTLAMQSLDLNTIARMVVDEMQARSESENRPMTFTLDIPADL
                     PPVLGDAERVQQILANLVRNGYIYTPPGGEVQIHMKALDGEVQVDVVDNGIGILSENQ
                     HRIFERFYRGDDPLVLASAGTGLGLAMAKTLVEMHHGRIWFASAGVSGEGSVFSFTLP
                     VYAQEEA"
     gene            12342..12716
                     /locus_tag="PKL11_04045"
     CDS             12342..12716
                     /locus_tag="PKL11_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ12806.1"
                     /translation="MAKILIAEDEKDIRDLITFVLTFAGHQVTAAANGEEAWLAAPTV
                     MPDLIMMDVRMPRMTGYEACEKMKADPRVAHIPVVFLSAKGQESEIQTGLSLGATEYL
                     LKPFAPDDLTRRVSELLAKYSK"
     gene            12744..13532
                     /locus_tag="PKL11_04050"
     CDS             12744..13532
                     /locus_tag="PKL11_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ12807.1"
                     /translation="MSVIVLNGSDMVHYEVLGRGRPLIFLHGWVGSWRYWIPSMQAAS
                     ISFRTYALDLWGYGDTTKNHRYSLADQSQLLDGFMDYMGIGRVALVGHGLGALVAVYY
                     ALTHADIVDRVMAVSYPMDESMVNPRIRTSPPADLAAWLLGKTPLTEPVLTDAPKADG
                     QAIQASMEDYSANGLKDIFKTLGTACLFVNGENDPAVQPPRTEQMAEMSEQTHAVLFE
                     QSGHFPMLDESSQFSRLLMDFLALTSGESPRDLQLKEEWKRRVR"
     gene            13685..14920
                     /locus_tag="PKL11_04055"
     CDS             13685..14920
                     /locus_tag="PKL11_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ12808.1"
                     /translation="MGKISSKQGISTLVNHVAEGHNPMHAHVTPIFQTSTFSFPDVAT
                     GASIFKGEEPGYVYTRIANPNQAQLSAKLAVLEGLDLLRARPDEAVEAVVDAHVFATG
                     MAAVTTAILARVRGGQTIIAQKALYSATYNFLQELAPRYGIRVVWLEDDLSLPRWEEA
                     FAANPDAVLAYAETPANPTMAVVDLAALSELAHRHNAWVMVDNTFATPYCQRPLTLGV
                     DVSIHSTTKYLSGHGVVVGGAVISRHVNWVRGELFSVMKILGGTPSPMDCWLTSIGLK
                     TFELRMQKHCENAMQVAQWLEAQPQIARVFFPGLPSHPDYAIAKKQMLSFGGMISFEL
                     KGGLAAGESMMNRVQLATLAVSLGNVDTLIQHPASMTHAAMPAAARLAAGITDGLVRL
                     SVGVENVEDIIADLAQALA"
     gene            complement(15059..15391)
                     /locus_tag="PKL11_04060"
     CDS             complement(15059..15391)
                     /locus_tag="PKL11_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12809.1"
                     /translation="MEKRFKILRFIGSMYKILGIISAILTVIGSVGSCLSFALAGGLS
                     SQLLEQYGTQLPAGAGIGIGIAAGLGILLGGLISALTLYAFGEAVYLFIDLENNTRTT
                     TELLRSRM"
     gene            15491..16432
                     /locus_tag="PKL11_04065"
     CDS             15491..16432
                     /locus_tag="PKL11_04065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ12810.1"
                     /translation="MTRPTISIIAPVYNEHESLPELIRRVHEVMDPSGETWELVLVDD
                     GSSDGSTDIIRTLAEQDKLVRPVIFARNFGHQIAVTAGMDYSRGDAVIVIDADLQDPP
                     EVMLEMIAKWREGYEVVYAVRAEREGETWFKKFTAATFYRLIYKITDVKIPLDTGDFR
                     LLDRKVVNVMGQMREHHRFLRGMSSWIGFKQIGVSYRRAARFAGETKYPFKKMLKLAM
                     NAITSFSYVPLQAASILGFICAGISLITIPVVIIARAAGSEAFFGQASTLLAVLFLGG
                     VQLISLGILGEYIGRIYDEVKGRPLYVVRQAPDKDEK"
     gene            16448..17926
                     /locus_tag="PKL11_04070"
     CDS             16448..17926
                     /locus_tag="PKL11_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ12811.1"
                     /translation="MTKIDLTVLPERRERAIQRARERNIIIPTFAEMVNPALIPAGIQ
                     AELRNVGLWDIHPRNLFRITWNNQPQPSGGGFGGVNYVELPSSLTGTKARIVALVGKW
                     FPTGAHKVGAAFGCLAPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSALLACDSIAIL
                     PEGMSRERFDWLKKVAGEVIATPGSESNVKEIFDKCWELRGSGQDLMIFNQFEEFGNY
                     LWHYTVTGHAMEEALQAVMRPGDTYRGVALTTGSAGTIAVGDYLKERFPTSKIVASEA
                     LQCPTLLRNGYGAHRIEGIGDKHVPWVHNVRNTDMVVAIDDDAVVAIARLFNEPAGRA
                     YLLRMGVPAETVNQLGLLGFSGIANVLSAIKFAKYYELGETDVVLTVLTDSMELYHSR
                     IEEMHAEFGAYTELNAAADFARHLLGVTTDNLLELSYAERRRVHDLKYYTWVEQQGKT
                     YEEILAQWYDPTYWTDLHGQAAEIDALITDFNERVGLIQKYR"
     gene            17932..18501
                     /locus_tag="PKL11_04075"
     CDS             17932..18501
                     /locus_tag="PKL11_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12812.1"
                     /translation="MDAWPPSTALILVGVMPSPRDLEIARVLGWYRIPLRTAPKVVAV
                     DYLAFYQTAAFGEDHGARIECVAPVRGHELTTRAELLRAEPDHPRAHEEYYKLLLGPL
                     QTLPRPITSGAWRRLTFLYTTGELLQSAQTLDDLVVRSDERALLWASLRERAAQAGQY
                     HAADLPELPLDPAILLMLGGLGDVGEPGE"
     gene            18619..>19774
                     /gene="hydA"
                     /locus_tag="PKL11_04080"
     CDS             18619..>19774
                     /gene="hydA"
                     /locus_tag="PKL11_04080"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNZ12813.1"
                     /translation="MRKLLIRNGTLVTAAELYPADILVIGEQIAAIGQNLPADDAEVV
                     DAAGKLILPGGVDVHTHFDLPMFGTVSSDDHYTGHKAAAFGGTTTVIDFVSQDFPSLS
                     DSIAAWHAKADPKAAVDFGFHMNITHLDAAIEAEMADLPGLGITSVKVFTAYNNRLRL
                     SDGDIFRVLRLGAQHGLLTLMHAENGDVIDLLTAEALAAGYTAPEWHAYTRPSWGAVE
                     ASLRGAAFAAQADAPLYIVHMNTGGEVDMLQYARQKGLPVMGETCPQYLFFTVNDLRR
                     PDGAKWVCSPPMRTSMDNTRLWQGLMDGTIQVIATDHCPFFFDGAQPITYEGQPIAIP
                     GKELGQSDFTKIPNGLPGVGDRLPILWTHGVLRSRLTPSQFVALNCTNPAR"
BASE COUNT         3909 a         6327 c         5361 g         4177 t
ORIGIN      
        1 tcgtcctctt caacgtcttt ggatattgaa ttactgtgat tataggccgt ttgatggcac
       61 tgtcaagagt gaatttctcc ggttcaccgt gggacttgaa acccgtctga atccatccca
      121 ttttcatttg acgtcaaatc ctcatccatg tataatcaac ctgcaggtat cctgttttct
      181 cagggcgaga tatggcgtta aaaggcaatc ttcgcgactt ttcgataacg cagttactca
      241 acttaataaa tttagcacga aaaaccggat tactggtcat tgaaggttcg ggtgaggccg
      301 cgcgcattca cttccgggat ggcaagctgg cttacgccca atatgccgtt gaagacaatg
      361 gtttggcggc catcctgcag cgcaatggca agatttctgc cagccagtat caaatcctca
      421 aagagcgcgc cggccacatg accgataaag aactcgggct catgctggtc aatgctggct
      481 atctctcaca aaatgatatc atcgagagcc tgcagcacta cttcatccag gtcacccggc
      541 gcctgttcac ctgggccgat ggtttcttca actttgacgg cgaaaatccc ccgcccgata
      601 accgcatcct ggtgcgcgtt gatcttgaaa acttgatcat cgaaggcgcc cgccaggtca
      661 aagaattaga acagctccaa gaagaaatcc ccagcctcga tatggcgctt aaattcactg
      721 accgccccgg cgccaatatc cgcagtgtca atctcagcca ggatgaatgg cggattgtct
      781 cctatgttaa tcccaagaac accctgcgcc agatcgccca ggccgcccgc ctgaacgacc
      841 tggagatccg ccgggtggtc tatggcttgc ttcaagccgg cctggtcgaa ctggttcgcc
      901 ccggcggcgc ccccctgccg ccctcaggtc ggatgttccc cacctccgac cggggagaac
      961 aacgctcgct ggtcaacaaa ttgatcagcc gcatccgttc gctctaatct tatttgggaa
     1021 ggatttgtta tgcaaaccgt aaaaattgtg gtaacaggcc ccttcaactc tggtaaaacc
     1081 gagtttattc agacggtcag cgagattgac gtggtctcaa cggagcgccg catttcctcc
     1141 gaagccgaac gggtgaaaga gaccaccacc gtggcgatgg actttggccg catcaccgtg
     1201 gacgaagacc tggtacttta cctgtttggc acaccgggcc aaaaacgttt tgacttcatg
     1261 tgggaaatcc tctctgaagg catgctgggc tttattgtta tggtggacag cacccgcccc
     1321 gaaaccttcc gcgaggcccg ctccattctg gaaaccttcc gggcctatgc ccccacccct
     1381 tacgttgtgg cggccaacaa acaagaccgc cccgatgcct gggaactgga agacatgcgc
     1441 gtagccttgc ggctcgacag caaggtcaaa ctgctgccat ccgttgccgt ggaccgcgag
     1501 tcggtcaaat cggtactgct tgaactgttg tacagcatcc tcgctgagat ggactccaac
     1561 cccggccgtt aggccagaga gacagttctg ccgtgtcgtc aatcactaca gatcagggca
     1621 ttttgcacta tgaggtttac gggcgcggaa agcccgtcat cttgctgcac ggctggctgg
     1681 gctcctgggg cctctggcag gagacgatga ccttcctggg ccaatattac cgcacctatg
     1741 cgctggattt ctgggggttt ggcgaatcgg gccgcaagct ggatacctac ctggtgaacg
     1801 attttgtcgc cctggtggac cagtttatgg acaagctggg catcgagagc gcccccctgg
     1861 tggggcattc catgggcggc acggtcagcc tgctggtatc cctgcgctac ccgcagcgcg
     1921 tgcaaaaagt caccgtagtc ggttcgccga ttgctggctc atcgctggcc tttctgctaa
     1981 agatggcggg cattcccttc aacgcttcag caatttttac cttctttggc ctgttccggc
     2041 gcatcatgcg cgctgccggg cccttcatct gccgagaccc gcgctttatg gacatgatgg
     2101 acagcgacct gaccaagctg acgctggaat ccttcttctc cagcattgca tcgctgcgcc
     2161 gcaccgacct gcgccctgaa ctgggcaatg tgcaggtgcc ggtgttgggc atgtatggcg
     2221 atcgggataa tatcgtcaat cccaatcagt ggaaattaat gccgcgcatc ccgcggggca
     2281 cctacctgcg ctttaagaat gccgggcatt ttattatgct ggatgaaccc cagcccttca
     2341 aaacccaatt gaaagacttt ttggacagcc cgctcccggc acccatcatc acctcctccc
     2401 caaacgaggt tcagcttggc ccatcgctgt aatcaatcac tcaccctggc gattatccgg
     2461 gggatgaaag aggtgattgg acagaccggc gttcaggccg tgttgaatct ggcgcagcga
     2521 gagcaggttt tatccgcacg tttcaacctg gatgacccgc gcggtctgct gtttaaagac
     2581 ctggcggccc tgcacagcat attagaggtt tcttatggcc cccgcggcgg gcggggtgtg
     2641 gccctgcgcg ctggacgggc ggcttttggt catctgctgc gtcagacggg cgatcagatt
     2701 ggcctgactg atatggaata tcgcctgctg cccacgcccc agcgcattca gactggcctg
     2761 caaaaactgg ctgccctcac ggcagcaagc tgcgcccaac cggttgaggt caccgatact
     2821 gaaaccgcct gggtttggcg catgtcgctg tgccccgaat gttatcgccg tcaggcggcg
     2881 gaaccggttt gccattttgt taccggatta ttacaagaat ttatggcctg ggccaacagc
     2941 ggtcgttttc accatgtaga agaaatcgaa tgccgggcca ctggcggggc agcctgtgtc
     3001 ttctcaattg atcggcagcc cctggagttg ggctgaacat gctaaaaatc atcctgcaat
     3061 gcccccgata tctggctccc ttcaacgaaa gagcgcgcga cctgcgcatc cagaaccaac
     3121 ccctctggct gcatcagcgc gatctgctgg ctggcattac cacaaatgag atggaactgc
     3181 cgccgggcgc ccgcctgccg caggttcacg agccttgcct ggtctatcgc gacaacctgt
     3241 tctttgatgc cgactttctc aatgcctttc tggctgctgc ccgcaaacgc caaaccccca
     3301 gccgcgccgc catctctgcc aacgacccgg ccttccggga gcactgcctg cccctctcgg
     3361 tttcctacac ccccgctggc gatatgtacc tggtggacct ctggtactat cccgatggcc
     3421 cggtgcccaa tgccgagccg ctggtggtgg acatgcagtc tcgtgagctg ggctactacc
     3481 atgtgcccac ctacatggcc aacgaaaccg gtgacctcac ctatcagatc ccgctgcgct
     3541 ctttgatcgc gattgattcc tgggttcaca tcttcatcgc tgactgtgtc tttggccttt
     3601 ttggccgcgg cgcccgagtt gaaaaacgcc tcgacgaaga cctggctttc aaactgaagg
     3661 tcattggcac ttcgttgtat gaagggcggc aggtgctgca gtgctccagc ctggtcaaaa
     3721 ttggccgcaa ctgctccatt gaccccgatg ctgtcattca tggccccacc accatcggcg
     3781 ataacgtcac cattggcgcc ggcgcggtca ttgaaaactg catcattggc gacaatgtca
     3841 acgtctcgca ggactgccag ttgatgctct cagtcgtcgg cgatggcacc ttcctgcctt
     3901 tccgcgccgc actgttcatg accaccatca tggagcacgg catggtggcg caaaacacct
     3961 gcctgcaaat gtgcgtggtt gggcgcaact cgttcatcgg tgcaggtaac accttcacag
     4021 acttcaacct ggttggcggc gccatccgcg cccgcgatgc caatggcgaa ctcaagccct
     4081 ccaaccgccc ggtgctgggc ggctgtgtgg ggcataactg ccgcctgggt tcgggcctca
     4141 tcttctatcc cgcccgcatg attgaatcag acgtggtcat gtttgcatcc aaagagcggc
     4201 gcgtggtgga ccacgacatt gtctatgaag agagcgacca cctgcgtctc aagaatcccg
     4261 gcattcatcc ccggctctat ccgcgtcccg gtgaagaggt agaagagacc tggtaagggg
     4321 caacttaata tttttgtaat ggggattcta tgattaattg ggctgcctcg ctcccactaa
     4381 aattaaaatt cgtgtacaat attagagtga ttgactctaa aagagagtcg gtctctccgc
     4441 tgcttttgtt gactatcagg gatttcattc acggaggccc ccatgggtaa agcccgacta
     4501 ttgatcgtag aagatgatgg cgacatctcc aatatgctgc gtatttattt caccagccag
     4561 ggatatgatg ttgacctggc cccgcgtggc tcagatgcgc tcgaaaagac tcgtcaggtg
     4621 atgccgcacc tgattgtgct cgatattatg ctgcccgata ttgacggtta cggggtttgc
     4681 cgcacactgc gcatgaacac ccgtaccagc catatccccg tcatctttct cacccagaag
     4741 gatgagcgca gcgaccgcct ccaggggctg gaacttggcg cagatgacta catcaccaaa
     4801 ccatttgaca ttgaagagct gaagctgcgg gttcaaaacg ccattgcccg cgcggagcgt
     4861 gagagcctca ccgacccgca atctggcctg ccggctggtc gcatgatcga agaccagctc
     4921 cgccgcatga tccgccagaa gggctgggcc ttcatggaca tccgcatcaa ccactttgat
     4981 cccttcaaag atgtgtacgg cttcattgcc gccaacgatg ccctgcgctt caccgccatg
     5041 ctgattggcg aagtggcaga cgaactgggc aacgacagcg actttgttgg ccatgctggc
     5101 ggcgataact tcgtcctcgt caccagtgaa gaacgcgccg ccgccattcg gcagcgcctc
     5161 aaggaacggt tcgccgaagc cgtgctggcg cactactcct tcatggatcg ccagcagggc
     5221 tacatcaatg tcaccgataa aaacggtcag accacccagg ccccgctgat gacactggcc
     5281 gtcggcgttg tttcgccaac cacccactcc ttcgccgata ttcgcgagat tacggaactg
     5341 gctgcagaag aacgtcggcg cgatactcag taagcctgac tgaggccgaa tgtttaataa
     5401 tagcgagtac gttctggcgg tgctttatat cctggcagcc ctgcttttgc tagggctggt
     5461 ctggatatta ttgcgttccg cccgccggct gcgccccatt ttgcaggccc gcccccccgt
     5521 ggatttgccc gtcacccctg ccgaaaccca caccgatgcg gtgattgtgg ttcaggctgg
     5581 tggacgggtc atgtccatta acgcggcggc ccgacagttg tttggcctgc agcccggtga
     5641 atcagctaac ctggaacgtc tcgcccgccg tttgcgcccc agtgaaccct ttatcaacct
     5701 ctgcgccgct gaggggcagg ctcgctttgt gcttgaaggc aaggccctgc agggcgtctc
     5761 atataagctg gcgctggaac ctgagccagt ggtggtggtt tcgctgcggg ctcccggtga
     5821 agaatcccgc tcgggcggtt cggcggccct gcagaagctg caaaacgcgc cctctttcct
     5881 ggctgccatg tctgccgata tggatattga cgccaccatt ctggctattc tccagagcgt
     5941 tgaccgcctg attgactcgg atgtggttga aatgaccatc tgggatgccg gaaaagagca
     6001 tctgacgccc taccgcatgg ttgccacccc cggcgccgag ccccggctgg ataaacccac
     6061 ccagcgttac cgcatgggcg agggcttttc cgggcagttg atccgcgaaa aacaacccct
     6121 gctgattacc gataccacca cccgccctga actgcgtcag gccgctgacc cggtttcttt
     6181 cccgctgcgt tcttatgtgg gtatgcccct gctggtcggc tctgaattta tcggcacgct
     6241 ggaactgggc gcagtcaccc gcggcaactt ccagcaggaa gatctggaac tactgcaatt
     6301 actggccccg caggccgcgg ttgccatcca caacaccctg ctctacaatc gagaacgcta
     6361 ccgcacggcg gaactggccg gactggccgg gctggctcag gccttcacca ccgtcagcga
     6421 cccctcttcc ctgtttgacc gcctgctcaa aagcatctcc ccgctcatcc cggtgcaaat
     6481 tctgggcttt ttgctctaca atgagaccac ccgccggctc gagggtcagg cgcccattat
     6541 cggtctgccg gaacaattta tggacctcta taacaccgag gtcgcgcccg acagcccgtt
     6601 ggaaaaagcc ctgctcgacc aggatctgat catcaccggc aacgccatgg aagacccgca
     6661 gtggcaggtg ctgggtatcg accaactggc gcgcggcgcc tcgctgcgcg ataccgccct
     6721 gatcccgctc aacgccagcg ggcacacgct gggctatttg caggcttcca accataccga
     6781 cggcggtgtg accttcaccc aggatgaact tcacctgctg ctgattgtcg ccaatcaggc
     6841 cgcctcgatt atcgaaaatt cacgcctggt gcagcaatcc cgcctgcgcg cccagcgcgc
     6901 cgaggccctg cgccgcatta ccgggctggc ttcttctgcc gccacgctgg atgaaatctt
     6961 ccagtactca ctgcaagaac tggcccgcct gctgcatgcc gacctgggcc tggcttttgt
     7021 cctggaccaa aaccgcaagc agttgagcct gctgcagtcc gccgttattg gcgaacttcc
     7081 tgacctgcca gagcgttccc gcaccctgct ggtagaagat gcccagtttg ccttcaccgt
     7141 cactggcgcc caatccacca tcaacacccc cgagctgact gccgaaacag ccatcatccc
     7201 gttttatcaa caaatcttca aagccgccgg tgtgcaggcc ctgttgggcg tgccgctggt
     7261 tgttcgagat gaaggcgtgg gcgaactgtg gtttgccagc cgcacccccg attccttcaa
     7321 tcaggctgat gtgcaggtgc tgaccaccgc cgccgggcag cttgccggcg tggtagaaca
     7381 ggccttcctg gtcgcccaga ccgatgccag cctgcgccgc cgggtggagc agatgaccgc
     7441 cctcacccgc atcagccgcg aactcagcac ctcgctggat ttgcaatccc tgcttcaact
     7501 ggtttattct gaggccctgc gcaccacccg cgccgactgc ggcaccatca ccctgtttga
     7561 cttgagccgc ccggaagccg aaaccccgcg ctcgcgcttc tcggttggcg atccggtggg
     7621 gcgggaactg tccgcgctgg aattgaaagt tctggctcag gcccaaccct tgaacctgcc
     7681 cgatgtcacc gccgaaaccg gctgcatccc ttcgcatgaa ggtattgcct cagccatgat
     7741 tgtgccggtt atctaccgcc agcgtcccgc tggtttgatc accctgcacg gcaagacccc
     7801 ggcgcgcttt gatgaagacg ccgttgaaat cgccaaatct ctggcggctc aggctgccat
     7861 tgccctgggc aatgccttcc aatttgaaga agaatcccgg cgcggtgaac tgcttcgccg
     7921 ccaggtggat accattggca agctcttcca ggtctccaag gtgatgcgcc ccggccaaaa
     7981 tgtagaagaa gccctgaccg ccattgctaa cgccatcagc gagaccagcc gcttcaaatt
     8041 tgtggtcatc agctcggtgg ataatgccgc cggcaccatg cgccgtctgg ctaatgccgg
     8101 gcacccgccg gaagtctggg ctgaaatgcg tacccgccgc aacccctgga atgtggtgga
     8161 aaccctttgc cagccagagt ttcgctttgg ctcctgcttc ttcatcccgg cagatcgccg
     8221 cccggtagtc actcaggatg tccattttgt cagcatgctc cccgccgaag agacccacga
     8281 gcaagatgcc tgggatacca aagacctgct ctttgtgccg ctgtacgatg ccgaaggtct
     8341 gccgcttggt ctggtcagcg tagatggccc ggtggatggc cgccgccccg accgccccac
     8401 cctggaagcc ctggaactgc tctccatgca ggtcggtgtg gtgctggaaa acagccgcct
     8461 caaccaggct ttgcaggaac gcactgaaga cctgcaaacc cgaaccgacc ggctggaggc
     8521 cgccctgtct ggtgcccagc agagcctgcc gctgctgctc aaccgccacc tcgaacaaac
     8581 cattaccatt caatcgctcc accgccagaa ccagcgcgtt caagatggcc tggccctgac
     8641 cgagcaggtt actacccaga atgatgctcc cagcctgctg cggacgctgg cgcgccagat
     8701 tttgacccgc tttgacctcc agaccgcctt gattggtgaa cggctggggt ctgaactgca
     8761 cattgtggaa gtgattggcg cagctcccgc gggtgccaac ccgcaggccc tctttggtca
     8821 aaagaacccc ctgcgtcagg ccttgcagga cgggcagccg ctgctggtct ccatgctgga
     8881 gcgcgagcct gagtggaaaa atatcccgct gctcaatgcc tttgccgccc agagctttat
     8941 tgccctgccc ctgccggtga ccgaggaccg ctcactggcc ctgctggcca ttggcaccga
     9001 ctcgctgccc gaatttacca ctgaggatcg tcagtcttat gcccggctga tccggcaggt
     9061 taccatcagc ctgcagaacc tggaactgct gacggagacc cgccgccgcc tcaacgagat
     9121 gaacctgctg ctcgacttca gccgcaagtt gggctctctc gaccccgcca gcatcctcaa
     9181 agccctgctg gagaacgccc gcgaagccat tccggtgggc acggctggct gggtggggct
     9241 gctcaacaaa gaaggccagt tggaaccgct ggccgccagt ggttatgccg attcagatgc
     9301 actgctggaa atgcgcttcc cgctggaggt ctctgggcgc agcctgccgg tcagcgcctt
     9361 ccgcgccaat cagccgctgc ggattgatga attgaacttt gtccaggaat acaccctctc
     9421 atccgatgac ctgctgcgct atcgccgcgc caccggcggc ggcttgccca ttgctgccct
     9481 ggcgaccccc attggcctgg gcgaacatgc cctgggtgtg ctggttctgg aaaacttcac
     9541 cacccccgac gccttcattc ctgaagacga gggcgtgacc caatccctct cccagcaggc
     9601 tgccttggcg ttggataatg cccgcctgct gcaatctgcc gagcagcgcg ccgcccagct
     9661 tcaggcgctc accgaagttg cgggcaccat tacttccagc ctgcaaacca acgatttgat
     9721 tgcttccctg ctcgaccagt tgggcgccgt catcccctat aacaccgcca cactctggct
     9781 gcgctctggc tccgatgtga ccgttgcggc tgccagcggc tttgccgata acgaaagccg
     9841 cctgggcctc tcggcccgcg tggaagacag cctgctcttc caatccatga tcgccactgc
     9901 gcaacctatc tccgtgcccg atgtgcgtga agatgtccgc ttccccagcc tgttggaacc
     9961 cgatcacttc tcctggctgg gtattcccct gttagccaaa ggtcaggtca ttggcctgat
    10021 tgccctcgag aaaaccgaac ccggtttcta cacccccgaa catatccagg gcgccaccac
    10081 ctttgccggt caggccgcag ttggtctgga aaacgcccgc ctgtttgaag aatccgtccg
    10141 tcgtgccggc gaactggacc agcgttcaca gcgtctggcg ctgctcaacc ggctctccag
    10201 cgaactgggc gcctcactgg atgtcgacta catcctcaag atcacctgcc agcagctgct
    10261 tagcgccatg ggcgccaacc gtgtcggcgc ggtgatgctg ggcgatggcg ggcggctcac
    10321 cctccaggtt gaggttccgg gcgctggcga acgcctgccg cggcagctgc ctgccacccc
    10381 gctcttcacc cgcctgatcg aatctcaggg cctcttctct accgccgatg ccgccgccga
    10441 agccgacctg ctgcccattt acgaaaccta tttgagccaa cggcataccc gctcactcct
    10501 catcgtaccc ctgatgaccg gctccaccct gcacggctgg ttgctggtcc aatcggttga
    10561 agtctatcgt tacagcctgc ccgaactgga actggcccgc accatcagca accaggccgc
    10621 cattgccatc cagaatgccc gtctcttcct cgagaccacc cgcctcaagg aagacctgga
    10681 gcgccgcgtt gaagaacgta ccgccgagct cagccgggag caccgcaatt cgcaaacgct
    10741 gctgaaaatc agcaccgagc tttcggccag cctcgacctg gatcaggtgc tggtgcgcac
    10801 gctgtctgtc ctcaacgagt ccattagcgc cgagcaaagc ctgattgtgc tgaccgcccg
    10861 accccaaaaa tattctgcgg gcgaatccct ggtggatgtt gccgctctga cgagcgattc
    10921 ccccgaacaa caggtggcta aatgggtcgt caagcgccgc tctgccgccg ttattgatga
    10981 cattgccacc gatgcccgct ggcagttccc agcggatcac ccgtttgctt ataagagcgc
    11041 gctggcaatt cctctcaccc tgggcgaaga tgtactcggc gcgttgatga ttttccaccg
    11101 ccagggtagc ttcttcatcc tggagcaaat tggcctcctc gaagcggctg tccgccagat
    11161 cagcattgcc ctcaacaatg ccgaactctt caacctgatc cgagatcagt cggaaaacct
    11221 gggcggtatg ctgcgcgagc agcaaatgga agccagccgt tcccgcagca tcctcgaagc
    11281 tgtggcggat ggtgtggtgg tcaccgatgc cagcggcgcc gtcacgctct tcaacccctc
    11341 ggcggaacgc attttggacc tcaaagccga tgatgttttg aagcgcccgc tctctcaatt
    11401 tgtgggcctg tttggacatg ccgccggtgc ctggtttgcc accattcgca cctggtcgca
    11461 aaaccccgcc acctaccagc ccggtgatat ttactctgag cagatggacc tggataatgg
    11521 ccgagttgta tccgtcaacc tggccccggt attctggcgc tcgcagcttc tgggcaccgt
    11581 ctcaaccttc cgcgatatca cgcatgaagt ccaggtggac cgcttgaagt ctgagtttgt
    11641 cgccaacgtc agccatgaac tgcgcactcc catgacctcc atcaagggct atgttgaaat
    11701 tatgctcatg ggcgccaccg gcgaactgaa cgcccagcaa aagcacttcc tccaggtggt
    11761 caaaggcagc actgaacgcc tgaccattct gattaacgac ctgctcgatc tctcccgcgt
    11821 tgaaaccggc cgcgtcaccc tggcaatgca gtctcttgat ctgaacacga ttgcccgcat
    11881 ggtggtggac gaaatgcagg ctcgctctga gagcgaaaac cgccccatga ccttcacgct
    11941 ggatattccc gccgacctgc cgcccgtgct gggcgatgct gaacgcgttc agcaaatcct
    12001 ggccaacctg gtgcgcaacg gctatatcta caccccgccc ggtggcgagg ttcaaatcca
    12061 tatgaaagcg ctggatggcg aggttcaggt ggatgtggtg gacaacggca ttggcatttt
    12121 gtccgaaaac cagcaccgca tctttgaacg cttctatcgc ggcgacgatc cgcttgtgct
    12181 ggcttcggct ggcaccggcc tggggctggc aatggccaag accctggtcg aaatgcacca
    12241 cggacgcatc tggttcgcca gtgcgggcgt cagtggcgaa ggcagtgtat tctcctttac
    12301 gctgccggtt tatgctcaag aggaggctta gggacttctg gatggctaaa atattgattg
    12361 ctgaagacga gaaagacatc cgcgacctga tcacttttgt gctcactttc gcggggcatc
    12421 aggtcacggc ggctgccaat ggcgaagaag cctggctggc tgcccccacc gtgatgccag
    12481 acctgattat gatggacgtg cggatgccgc gcatgacggg ctatgaggcc tgtgagaaga
    12541 tgaaggcaga cccgcgcgtg gcgcacattc cggtcgtctt tctctctgct aaagggcagg
    12601 aatccgagat tcaaacgggg ctcagtctgg gcgccaccga atacctgctc aaaccctttg
    12661 cacccgatga tctgacccgc cgggtgtctg aattactggc caagtacagc aaataatcac
    12721 acgaggataa caggaattct ggcatgagcg tcatcgtttt gaatggttcc gacatggtgc
    12781 attacgaggt gctggggcgc ggccgccctc tcatcttcct gcacggctgg gtcggttcct
    12841 ggcgctactg gattccctcg atgcaggctg catccatcag cttccgcacc tatgcgctgg
    12901 acctgtgggg ctacggcgat accaccaaga accaccgcta ttcattggcc gaccaatcgc
    12961 agttgctgga tggctttatg gactacatgg gcattggccg ggtggcgctg gtggggcacg
    13021 gcctgggcgc tttggtagca gtctattatg ccctgaccca tgccgatatt gtggaccggg
    13081 tgatggccgt cagctatccc atggatgaga gtatggtcaa cccgcgcatc cgcaccagcc
    13141 cgcccgctga cctggcggcc tggctgctgg gcaagacgcc gctgaccgaa cctgtgctga
    13201 ccgatgcccc taaagccgat gggcaggcca ttcaggcttc aatggaagat tattcagcca
    13261 atggcttgaa agacatcttt aaaacgctgg gtaccgcctg tctgtttgtc aatggcgaaa
    13321 atgaccctgc agtgcagcca ccgcgcaccg agcaaatggc cgaaatgtca gaacaaaccc
    13381 acgcggtcct gtttgagcag agcgggcact tccccatgct ggatgaaagc agccaattca
    13441 gccgcctgtt gatggatttt ctggccctga cctctggcga aagcccgcgc gacttgcagc
    13501 tcaaagaaga gtggaagcgc cgcgtacgct gatttttgcc gggtgtttgg cttccggtta
    13561 tgacaaaacg ccaatctcat cacgtcagtt gtcctacaat tagcctgctg ccaaaatgga
    13621 tggctgccgg ggtggtataa tctccgcgcg gcctgtccgc cattctatat tttaggagcc
    13681 caacatgggg aaaatctctt ccaaacaggg gatcagcaca ctggttaatc atgtggccga
    13741 aggccacaac ccaatgcatg cgcatgtgac ccccattttc caaacgtcaa ccttcagctt
    13801 ccccgatgtt gccaccgggg cttccatctt caaaggcgaa gagcccggtt acgtctatac
    13861 ccgcattgcc aatcccaatc aggcgcagct ttccgccaag ttggccgtgc tcgaaggcct
    13921 tgacctgctg cgcgcccgcc ccgacgaagc cgtcgaagct gtggtggatg cccacgtctt
    13981 tgccaccggc atggcagccg tcaccaccgc cattctggcg cgtgtgcgtg gtggccaaac
    14041 catcattgcc cagaaagcgc tttactccgc tacctataac ttcttgcagg aactggcccc
    14101 gcgctacggt atccgcgtgg tctggctgga agacgatctg agcctgccgc gctgggaaga
    14161 agcctttgcg gccaaccccg atgctgtgct ggcctatgcc gaaacccctg ccaaccccac
    14221 catggcagtt gtggacctgg ctgccctttc tgaactggcg caccgccaca atgcctgggt
    14281 gatggtggat aacacctttg ccacgcccta ctgccagcgc cccctcaccc tcggcgtgga
    14341 tgtcagcatc cactccacca ccaaatacct ctccggtcat ggcgttgtgg tcggtggtgc
    14401 ggtcatcagc cgccatgtca actgggtgcg cggcgaactc ttttcggtga tgaaaatcct
    14461 gggcggcacc cccagcccca tggactgctg gctgaccagt attggcctca aaacctttga
    14521 actgcgcatg cagaagcact gcgaaaacgc catgcaggtg gcgcagtggc tcgaagccca
    14581 gccccagatt gcccgtgtct tcttcccggg cctgcccagc cacccggact acgccattgc
    14641 caaaaagcaa atgctgtcct ttggcggcat gatctccttt gaactcaagg gcggtctggc
    14701 tgccggcgaa tcgatgatga accgtgtcca gcttgccacc ctggcggtca gcctgggtaa
    14761 tgtggatacg ctcatccagc accctgccag catgacgcat gccgccatgc ccgccgcagc
    14821 ccgtctggcg gctggcatca ccgatggtct ggtgcgcctc tccgtcggcg ttgaaaatgt
    14881 cgaagacatc atcgccgatc ttgcccaggc gttggcctga cccgtctctg aacgctttgc
    14941 tggcgcgcga actcgcacgc cagaccaaac caaaactcgc actccagaga atctccccat
    15001 ccccctctgg agtgcgagct ttagctcact gccatggcag gctaaaatgc cgggttgatt
    15061 acatccggct gcgcagcagc tctgttgtgg tccgggtatt attttccagg tcaataaaca
    15121 gatagaccgc ctcgccaaac gcgtacagcg tcagcgcaga gatcaacccg cccagcagaa
    15181 tccccagccc ggcagcaatc ccaatcccaa tgcccgcgcc tgccggcagt tgggtgccat
    15241 attgctccag cagttggctg gacagccctc ccgccagggc aaagctcagg cagctgccca
    15301 ccgaaccaat caccgtcaga atggcgctaa tgatgcccag gattttatac atcgagccaa
    15361 taaaccgcaa gatcttaaac cgcttttcca tgatattttc tccttctgac atcattatag
    15421 tgggaatcac ccaatgaggt ataatcaaag cggcatgttg tttgacaatt tcatttgatc
    15481 aaggagcatt atgacccggc caaccatttc gattatcgcc cctgtgtaca atgagcatga
    15541 atcactgccc gagctcatcc gccgtgtaca cgaagtgatg gaccccagcg gggaaacctg
    15601 ggaactggtc ctggtggatg acggttccag tgacggttca accgatatta tccgcactct
    15661 ggctgaacag gataaactgg ttcgcccggt gatttttgcg cgcaacttcg ggcatcaaat
    15721 tgccgtcacc gccggcatgg attacagccg cggcgacgca gtcatcgtca ttgatgccga
    15781 cctgcaggac ccgcccgagg tgatgctgga aatgattgcc aaatggcgtg aaggctacga
    15841 agtggtctat gccgtgcgcg ccgaacgcga aggcgaaacc tggttcaaga aattcaccgc
    15901 tgccaccttc taccgcctga tctacaagat caccgatgtc aaaatcccgc tggataccgg
    15961 cgactttcgc ctgcttgacc gcaaggtggt caatgtgatg ggtcagatgc gtgagcatca
    16021 ccgcttcttg cgcggtatgt cctcgtggat tggcttcaag cagattggtg tcagctaccg
    16081 ccgcgccgcc cgttttgctg gcgagaccaa atacccgttc aagaaaatgc tcaagctggc
    16141 catgaacgcc atcaccagct tctcctatgt gccgctgcag gccgcttcca ttctgggctt
    16201 tatctgcgcc ggcatcagcc tgatcaccat tccggttgtc attatcgccc gtgcggctgg
    16261 gtctgaggcg tttttcggcc aggcttccac gctgctggcg gtattgttcc ttggcggtgt
    16321 gcagctaatc tcgctgggta tcctcggcga gtacattggc cgcatttatg acgaagttaa
    16381 aggccgcccg ctctatgtgg tgcgccaggc ccccgataaa gacgaaaaat aactcaggag
    16441 attggacatg actaaaattg atttaaccgt actgcccgaa cgccgtgagc gcgccattca
    16501 gcgggcccgt gaacgcaaca tcatcatccc caccttcgcc gaaatggtca accctgccct
    16561 gatcccggcg ggcattcagg ctgaactgcg caacgttggt ctgtgggata ttcatccccg
    16621 caacctgttc cgcatcacct ggaataatca accccagccc agcggcggcg gtttcggcgg
    16681 cgtcaattat gttgagctgc cctccagcct gaccggcacc aaggcccgca ttgtggctct
    16741 ggtgggcaag tggttcccca ccggagcgca taaagttggt gcggcttttg gctgtttggc
    16801 cccccggctg gttaccggcc agtttgaccc caccacccag aaagccgtct ggccctccac
    16861 cggcaactac tgccgcggcg gcgcctacga ctcggccctg ctggcctgcg attcgatcgc
    16921 catcctgccc gaaggcatga gccgcgagcg ctttgactgg ctcaagaaag ttgctggcga
    16981 agtcatcgcc acccccggca gcgaatctaa cgtcaaagaa atttttgata agtgctggga
    17041 actgcgcggt tctggtcagg atttgatgat cttcaaccag tttgaagagt ttggcaatta
    17101 cctctggcac tataccgtca ccgggcacgc catggaagaa gccctgcagg ctgtgatgcg
    17161 ccccggcgat acctaccgcg gcgtagcgct gaccaccggc tcggcgggca ccatcgccgt
    17221 tggcgattat ctcaaagaac gcttccccac cagcaagatt gtggcctctg aagcgctgca
    17281 gtgccccacc ttgctgcgca acggctacgg cgcccaccgc attgaaggca ttggcgacaa
    17341 gcacgtgccc tgggtccaca atgtccgcaa caccgacatg gtggtagcca ttgacgatga
    17401 cgccgtggta gccattgccc gccttttcaa cgagcctgct gggcgcgctt acctgctgcg
    17461 catgggcgtc cctgccgaaa cagtcaatca actgggcttg ctgggcttct ctggcattgc
    17521 caatgttctc tcggctatca agtttgctaa atactacgaa ctgggcgaaa ccgatgtggt
    17581 cctcacggtc ctgaccgact cgatggagct ttaccacagt cggattgaag aaatgcacgc
    17641 tgaatttggc gcctacaccg aactgaacgc cgccgccgat tttgcccgtc acctgctggg
    17701 cgtaaccacc gacaacctgc tggaactctc ctatgcagaa cgccgccggg tgcacgacct
    17761 caagtactac acctgggtgg agcagcaggg caagacctac gaggagattc tggcgcagtg
    17821 gtacgatccc acctactgga ccgacctgca tggtcaggct gccgaaattg atgccctgat
    17881 tactgacttc aacgagcgcg ttgggctgat ccaaaagtac cgctaaccgc catggatgcc
    17941 tggcctccat ccaccgcatt gatcctggtc ggggtgatgc cctcaccgcg tgacctggag
    18001 attgcgcggg tgctgggctg gtatcgcatt cccctgcgca ccgcccccaa ggtagtggca
    18061 gtggattatc tggcgtttta tcaaaccgca gcgtttggcg aagaccacgg cgcgcggatt
    18121 gagtgtgtgg cccctgtgcg cggccacgaa ctgaccaccc gcgccgagct gctgcgcgct
    18181 gagcccgacc atccccgcgc ccacgaggaa tattacaaac tcttgcttgg gccgctgcaa
    18241 accctgccgc ggcccatcac ctcgggcgcc tggcggcggc tcaccttcct ctacaccacc
    18301 ggcgaactgc tccaatcggc gcaaaccctg gatgacctgg tggtgcgctc cgatgaacgc
    18361 gccctgctct gggcctccct gcgcgaacgc gccgcccagg cgggccaata tcacgccgcc
    18421 gacctgcccg aactgcccct tgaccccgcc atcctgctca tgctgggcgg tttgggagat
    18481 gttggagagc ctggcgaatg atcgcgccag gcggttaacc tctggttctt ccgtaggggc
    18541 ggacctgcgt gtccgcccgg aaaaccactc aggagcctca cttcagatcg cttctaaatt
    18601 cattcagaga ggtgtaatat gcgcaaatta cttatccgca atggcaccct ggtcaccgcg
    18661 gccgaactct acccggccga tattctggtg attggcgagc agattgccgc cattgggcaa
    18721 aacctgcccg ccgacgacgc cgaggtggtg gatgccgccg gcaagctgat cctccccggc
    18781 ggggtggatg tgcacaccca cttcgatttg cccatgttcg gcaccgtctc atctgatgat
    18841 cactacaccg ggcacaaggc cgccgctttt ggcggcacca ccaccgtgat tgatttcgtc
    18901 tcacaggatt tcccctcgct ttcagacagc atcgccgcct ggcacgccaa agccgacccc
    18961 aaagccgccg tggactttgg cttccacatg aacatcaccc acctggacgc ggcgattgag
    19021 gctgaaatgg cagacctgcc cggtctgggc attaccagcg tcaaggtctt caccgcctat
    19081 aataaccgtc tgcgcctgtc tgatggcgat attttccgtg tgctgcggct gggcgcccag
    19141 cacggccttt tgactctgat gcacgccgaa aatggcgacg tgattgatct gctgactgcc
    19201 gaagcgttgg ctgccggtta caccgctccc gaatggcatg cctacacccg ccccagttgg
    19261 ggcgccgtag aagccagcct gcgcggcgcc gcgtttgccg cccaggccga tgccccgctt
    19321 tacatcgttc acatgaacac cggcggcgag gtggatatgc tccaatatgc ccgccagaag
    19381 ggcctgcctg taatgggcga gacttgcccg cagtacctct tctttaccgt caatgatttg
    19441 cgccgccccg atggcgccaa atgggtttgc tcgccgccca tgcgcacctc aatggataat
    19501 acccgcctct ggcaaggcct gatggatggc accatccagg tcattgccac cgaccactgc
    19561 cccttcttct tcgatggcgc ccaacccatc acttacgaag ggcagcccat cgccatccct
    19621 ggcaaggaac tcggccagag cgactttacc aaaatcccca acggcctgcc cggtgtgggc
    19681 gaccgcctgc ccatcctctg gacgcatggc gtgctgcgtt cccgcctgac ccccagccag
    19741 tttgtggcgc tcaactgcac caaccccgcc cgca
//
LOCUS       DAOPVS010000026        18840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_2972_length_18840_cov_54.338781, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000026 DAOPVS010000000
VERSION     DAOPVS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2972_length_18840_cov_54.338781"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PKL11_04085"
     CDS             <1..805
                     /locus_tag="PKL11_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HNZ12814.1"
                     /translation="TDGDPLTAILVTGPEHGTLTLNSDGSFTYSPEDNWNGSDSFTYK
                     ANDGATDSNPAAVTLTVNPVNDAPVAADDTASTNEDTQVDIPVSTLLVNDSDVDGDAL
                     SLVEINNVTSGTVALVGETITFTPTTNFHGEAGFDYTITDDTTLDTAHVTIMVTPLND
                     PPTASNDSYSTNEDTPLTVATPGVLENDTDADGDLLNAILVTGPQHGTLTLNPDGSFT
                     YSPDIDWSGIDSFRYKSNDGLEDSNTATVTITVVSAIEWKFIYLPLVFK"
     gene            1314..1772
                     /locus_tag="PKL11_04090"
     CDS             1314..1772
                     /locus_tag="PKL11_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNZ12815.1"
                     /translation="MFSLTDAACLWQPAPGSWTVHLSEDGNWRPDWSMPEPKPAPATT
                     IGWLTWHLIWWWSGLIAAIRGEEPPDHAAVFWPGSAEAVILQLKLLSDQWADLLSQFQ
                     EADLERPLAYPWAEPRPLRIALAWANLELMKNIAEIGCIYRMFEAARSAE"
     gene            complement(1789..2850)
                     /locus_tag="PKL11_04095"
     CDS             complement(1789..2850)
                     /locus_tag="PKL11_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNZ12816.1"
                     /translation="MPNLFEPLTIRSITLRNRIGVAPMCQYSYMDGYSNDWQLTHLGA
                     RAAGGAGLIISEATAVEARGRITPHDVGIWDEAHIEPLRRVTDFIKAQGAVPGIQIAH
                     AGRKANTQRPWDGGKPVTAVQPDFWPVVGASPIPFSEGYQTPHELALDEIRIVQQAFK
                     AGAQRAAEAGFEWLEIHAAHGYLIHNFLSPLSNQRADAYGGSFENRIRFLIETIKQIQ
                     LVWPEHLPITVRISATEWVEGGWTVTDSVELARELINAGVDLVDCSSGGNIPKAQMPL
                     GPGYQVPLSEAVRATGMRTATVGLITEPEHADEIIRNGRADLVLLGREMLRDPHWALR
                     AAQTLKQPVPVPNPYLRAY"
     gene            complement(2924..4144)
                     /locus_tag="PKL11_04100"
     CDS             complement(2924..4144)
                     /locus_tag="PKL11_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNZ12817.1"
                     /translation="MAKKTQVGIIGAGPAGMMAALQAAWKGAEVTLFDANAIVGRKLL
                     VTGSGRCNLTNAAIAPERYVCSNPAFLAALFNQFDREALLKTLERIGILAYHTADGWY
                     YPCSESAATVVDAFAAALAQAGVRLRLNQRVTGLQKTDAGFRVQAGESEWIGERLIVA
                     AGGQAYPTLGSRGDLYPLLSRLGHTITPVYPALAPVQADVRMLHKLQGVRLDVTAGVW
                     QGKTLLGTTTGNLIFTQWGFNGPAVMDLSHLISTRPGTPLHLTLNLLPGESEPRLRAL
                     LKQKRQEAVPVRVLLGAVLPPKVPPVILGLAGINAEVQINQLTEAAVENILKQLTHIL
                     VEIQGVRGFDYAQVSTGGVPVDEVRPASLESKLVPGLYLAGEMLDVIGPCGGFNLQFA
                     FSSGAAAGWAAGQG"
     gene            complement(4205..4624)
                     /locus_tag="PKL11_04105"
     CDS             complement(4205..4624)
                     /locus_tag="PKL11_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12818.1"
                     /translation="MITVQLGTEDQIEAICALDEIARHNNERREFIQRAVAEKICYVA
                     ARPQIVGYAVLNYSFFAQGFIAMLYVQQDWRRQGVGQILLGYLEKVCQTHKLFTSTNL
                     SNLPMQRLLAKFNYRLSGVIHDLDEGDPELVYVKYLR"
     gene            complement(4627..5748)
                     /locus_tag="PKL11_04110"
     CDS             complement(4627..5748)
                     /locus_tag="PKL11_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNZ12819.1"
                     /translation="MRLKSCLLIALITALLTGCQTVPVETGNTPESESLIGLPGEGQL
                     YHGVYPGGITGEEDDLTLQDLRSYEEAVGKPAVWVFFSHNWYHGREFPTATAEWIRTA
                     GSIPYVRLMMRSDASLDHGEPVFTLERIINGDFDADLRNWADAARDFGTPIIAEFGTE
                     MNGEWFSWNGVWNGGGDLTGYGDPNEPDGPERFRDAYRHIIDISREQGAINILWVFHV
                     NGSDWPEDDWNHLENYYPGDAWVDWIGVSVYGAQTPLDEEWPDLRSQMDAVYPRLTAL
                     APDKPIILIEFGVTANNPLGDQAEWAEDALSDLTEQRWPRVIGFSWWNETWQNDDNPA
                     HDTNMRVQDNPQLASVFQQLVAENTAVLGEAILPVENGE"
     gene            5857..6708
                     /locus_tag="PKL11_04115"
     CDS             5857..6708
                     /locus_tag="PKL11_04115"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNZ12820.1"
                     /translation="MEELTTKPAPARPTIYIISGGVGASAEQLVYTVLAQYPEGAVDV
                     FTTGNVRTFEQILPVLEQAATKSAFIIHTLVDPSLRQQLIVAAAERQIPAVDLMGDLM
                     GWLTQQLGELPYIQPGRYRRLHRDYYDRVAAIDYTLAHDDGKHPAGWRNAEIVLLGVS
                     RSGKTPISLYLAVLGWKVANIPLVPRLDIADQVYTLDPARCIGLILDADRLLLYRRQR
                     QARLGVSAASDYTDIDEIELELRSAMKLYRRCGFQVVDTTDKTIEMSADEILRRIQNK
                     PPNALPV"
     gene            complement(6729..7937)
                     /locus_tag="PKL11_04120"
     CDS             complement(6729..7937)
                     /locus_tag="PKL11_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12821.1"
                     /translation="MTEKSFRLYGYRWVVLAVFMFINLTIQILWIAYAPITGVAAEFY
                     GVSDLQIGLLAMTFMMAFIPLSIPVSWAIDTYGFRPTVSIGAVLMGIFGVVRGLAGSN
                     YTLVLLSTIGIAIAQPFLLNAWTKVPAQWFSIEERATAVGLITLSNLVGTALGMVLTP
                     LLTESLSIPHVQLIYGGVAAFSAVLFLILAREKPATPPCPAGLEVRALMLDGLKHALT
                     VKEFWLYLFVSFIGMGVFNGITTWVENIIRPRGFTPTDAGTLGALMLVGGVLGAVLIP
                     PFSDRQHRRRRYILMGFVLAIPGLVGLAFASSAWLLFISAFAMGFFLISASPVGMQYA
                     AEVTHPTPEGTSNGLIQLFGQASVVFVYIMEALKTPDGSFTPALLVSIGLLVISTVLI
                     LQMKDPQPLK"
     gene            complement(7948..9528)
                     /locus_tag="PKL11_04125"
     CDS             complement(7948..9528)
                     /locus_tag="PKL11_04125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ12822.1"
                     /translation="MSDPNAPIILSIDLGTSGMKVALITIHGKVIGWDSEPIQLYLTP
                     DGGAEQSPDEWWGAFITAAQRLIGRGLAAPSRIKAVCCSTQGEGTIPVNRAGKALMNC
                     VLWMDMRGAANLRRQFGGVIKVSGLGAQHVLRWIGLTGGMPSATGKDPAAHMLLIRDR
                     YPEVYAQTYKFLNVLDFLNLQLTGRFTATVDSILTSWVTDNRNPDNIHYDSGLVRSCG
                     VAGDKLPEIVPCTAVLGPLVPSVAAQLGLSSEVKVVAGAIDNTAAAIGAGAIEDYIPH
                     LYIGTSSWLAAHVPYKKTDVFASLASVPCAVPGRYLLTALQATAGGNLTYLRDNIIYN
                     KDELLQEAQVPDIFKVLDQIAARVPAGANGVIYTPWIWGERAPVEDKALRAGLYNLSL
                     RNTREDIIRAFLEGIALNTRWVLEPMDRFMGRKTRSIHIVGGGAQSDIWCQIFADALN
                     VEIKQVAGPVYANACGAAWIGAVGLGEIGFNDIPGLVQFKRLYEPQQANTSIYDERFA
                     IFTRIYRQMKGIYHQLNR"
     gene            complement(9550..11004)
                     /locus_tag="PKL11_04130"
     CDS             complement(9550..11004)
                     /locus_tag="PKL11_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ12823.1"
                     /translation="MTENQAYAISKYPDVEDVYRRLNALVAQPIRPVRREKMEEYLGY
                     FQTRCARSKALTDEAKKLIPGGVQHNLAFNYPFPIAIEKVDGAHLWDVDGNRYIDFLQ
                     AGGPTILGSNYEPVRAKVIEMLQSCGPVTGLFHEYELKLAQLIQRLMPNIEMFRMLGS
                     GTEGVMAAIRAARAFTKKKKVIKVGGAYHGWSDSMVYGLHIPGTGRLEAMGIPGGATA
                     NTQEFFPNALGALKRQLILNRVLGGTAAVIVEPVGPESGTRPVPYDFNQKVRELCDEF
                     GALLIFDEVVTGFRLGLGGAQGYFGVKPDLTVFGKCLTGGYPMAGGVGGRAEVIMRFA
                     AGIGGTGQNAYIGGTLSANPISCVAGYHALLEMERTNAPVIAGRAGDRLTAGLQKLIE
                     KYNLPFVAFNQGSICHLETSGVMLLDLRNLIRLSKELKKRKHMLEEMGAAYLANGLIT
                     LAGSRMYTSMADTDEVIDEALARFEAVFSNIEGA"
     gene            complement(11037..11723)
                     /locus_tag="PKL11_04135"
     CDS             complement(11037..11723)
                     /locus_tag="PKL11_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ12824.1"
                     /translation="MTMFEDAKIQVVNAAQLLTRKGFLSATGGNLSVRIAGQKAFAIT
                     PSNYDYMKMVPEDICVLDFDLHPLAGERKPSVESGMHGAIYQARGDVNAIIHTHQVYP
                     STLAILNLPIPALFDEQVRFLGRSVDVIPYAPSGTGMLKNTVARHVSNHNNAYIMANH
                     GALLFGHDLERAMHNVEVLEKCALAYLLAVLSEKKVSKIPLPIRELAFAKLRKDQKRV
                     ESGNTQTGGE"
     gene            11811..12434
                     /locus_tag="PKL11_04140"
     CDS             11811..12434
                     /locus_tag="PKL11_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ12825.1"
                     /translation="MTHKQYHHGDLKNALIKAGTEILSEEGVAGLSLRKVAARAGVSH
                     SAPYAHFSDKQALIAAISTEGFRQLNARLSSISDACQTEPARLLLEVAFAYVQFALDS
                     SACFKLMFSGVLEKEKDYPDFVAISKASFQLLVTLVQRCQQVGLIRPGDPDVLAVSIW
                     SLVHGFTALLLERQISSHILDRFTIKALLCQTLNQVVLTEIQPVETD"
     gene            complement(12435..14303)
                     /locus_tag="PKL11_04145"
     CDS             complement(12435..14303)
                     /locus_tag="PKL11_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNZ12826.1"
                     /translation="MGRILYIHLLGEFRVIWNDVPIRRFDTNKARALLAYLAMGRGQR
                     HSREVLAEMFWPDVPTENALRNLRLTLHRLKRTLEVALRAEEEVKIFRTDRATIQLDP
                     DLMVWVDALKFQDLMTECADHSHENGGVSSECAQRLAEAVDLYRDAFLRNVDVSSSSS
                     FEEWMVVQRERFHRQTLEALTDLGRFHLKLAIDAITAPGRQAAFEKAIQYSRRALLLE
                     PWQETCHQTIMSALAMTGQVPAAIAQYRICCQVLVDEFEMEPGVETTQLYKKITSKRG
                     LGVQETSKPSSRGNLRAALTSFIGREAQADKLSRLIFAYRWVTLVGEGGVGKTRLVQA
                     VAETLIDQFEGGIWQLPLETHKQASTTRAEIHHTIKQMLIEYLPFLSGTPDMPADRLF
                     SQFGSAKRLIILDSFEYFSDGADIILELLNQAPQVRVVITTRQALYFQAGYIMRLEGL
                     VVPEENEAEAANAPAVQLFIERACSAGCEFDAGSPEDMRQIIQICNKLNGNPLAIELA
                     APWATRMTLAQINENIEEDPIVFPASLISDIPSRHSSMREVFESSWALLSEREQAVLK
                     LCATLEPEFTFADVFALDPLLTNELDQLADKSMLARMDAGRYQILEILRRLIRNGV"
     gene            14640..>16553
                     /locus_tag="PKL11_04150"
     CDS             14640..>16553
                     /locus_tag="PKL11_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ12827.1"
                     /translation="MNNPKPGSTMSNTVLKRMVQMMFAAIVVVVFVGVAMFTLPRQST
                     ASGSNQLGNAVPAAALISDAQPQAQPTPNSQKEVVPPDYAPGGGFYPPVVDQGGDCIS
                     GYVIDVYHQNAGAGWEIVVTSKETGQVWTQITGVDGHYSFSPLPAGTYVVEIKYREGW
                     RAFTLQSFEVTLSGEPGVCAVTRFKMEALPCLIVRKIDKNSAGLPNPVGLADWPITVR
                     NGDYSETKLTDWDGYAEFRYLVPGTWIVEEANQIGWIPEVGQPSSITLSSPRQPYTCE
                     RIVLVNKQLHTSCIAAKKVDDQGRPLANWVINLVRKDGTRPPRQTVTDANGYAFFLDL
                     ELGEWIVSEVVQDGWQPVGETSYTVNLDVPTTLCPTVTFINQPTGCIEGLKINHYDQG
                     LPNWRINATHTATGQQLSTITDINGYFKFENMLLGEWVIREEMQPGWEAVTPGELTVT
                     LTDPFKCEQIRFKNMTEFACLDVYKYDDTGAPLPGWTMILEPAYGGEQLTGITDGSGW
                     VRFNKLTPGEYYVTEIVQEGWDPITPIHQYVRVEATGTCRIMEFYNEQESPYCDDDCH
                     DDCDDDCDDDCSIDGCCNDCNNGCCDGCNNGCCNGCNNGCCTNCNNGCCNGCCDSTYC
                     TASSSEQIWNPPDP"
     assembly_gap    16554..16653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16877..17065
                     /locus_tag="PKL11_04155"
     CDS             16877..17065
                     /locus_tag="PKL11_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ12828.1"
                     /translation="MPSLPSDGSSDGDSSGTTTYVVKSGDTLFAISQAYGITVNQLKA
                     ANGLTSSLIYTGQILKIP"
     gene            complement(17131..17640)
                     /locus_tag="PKL11_04160"
     CDS             complement(17131..17640)
                     /locus_tag="PKL11_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ12829.1"
                     /translation="MELMEALLTRRSIRKFTGEPIGAEDLTAILRAGSYAPSAMNRQP
                     WHFVVLREPEMLEAITKVQPYTQMLPKAGCGIVVCGDKLRQPQVGFLVEDCSAAIQNM
                     LLAAHGLGLGAVWCGLYPVSIFTKPMGKLLKLPVNIVPVGLIAVGHKIEDRTVAERFD
                     PDRVHYEGW"
     gene            17715..17993
                     /locus_tag="PKL11_04165"
     CDS             17715..17993
                     /locus_tag="PKL11_04165"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNZ12830.1"
                     /translation="MSFWDILLWIVIGGLAGLLADWVVKGIRVGLLGAIIVGILGGFL
                     GGWLFGQLNIAIGSGFWGSLISAFVGAVILLFVLRALRRVFRTRRRRR"
     gene            complement(17997..>18840)
                     /locus_tag="PKL11_04170"
     CDS             complement(17997..>18840)
                     /locus_tag="PKL11_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ12831.1"
                     /translation="GKTVVITGATAGIGLAAACALAEQGARLIGVGRDPVRCEAAGKV
                     IQQANPNAVVQFLQADLALQSEVRRLATEIGETLTRWGVPGLDVLVNNAGMIASSLQR
                     TSEGIERTLAVNHLAPFLLTHQLLPWLQAARPGRVITTSSESHYRTWINPKRINHPLI
                     YHVLWAYKVSKLENVLFTIEFNRRVTPTRAYAVDPGLVNTEIGLKGTGLLSRWIWEMR
                     RRGGTPAEVPAQTIAHLACAEDLTDAPYWYNGAPKQPSRAARSEKAARELWEVSCELC
                     GIEW"
BASE COUNT         4252 a         5683 c         4491 g         4314 t
ORIGIN      
        1 cacagacggc gatccgctga ccgccatcct ggtcactggc ccggaacatg gtaccctgac
       61 gctgaactcg gacggcagct ttacctattc accggaggac aactggaacg gcagcgactc
      121 tttcacctac aaggccaatg atggagcaac cgattccaac ccggcggcag taacacttac
      181 ggtcaacccg gtcaatgacg ctccggtagc tgccgatgat accgcttcga ccaacgagga
      241 cacgcaggtt gacatccccg tcagcaccct gctggtcaat gacagtgatg tcgatggtga
      301 tgccctcagc ctggtcgaga tcaacaatgt cacgagcggc acggtggccc tggttggtga
      361 gactatcact tttacgccca ccaccaactt ccacggcgag gccgggtttg actacaccat
      421 caccgacgac accacactcg acacggctca tgtgaccatc atggtcaccc cgctcaacga
      481 tccgccaaca gcctcaaatg attcctattc aaccaatgag gatacgcctt tgaccgtagc
      541 cactccgggt gtattggaga acgacactga cgcagacggc gatctgctga acgccattct
      601 ggtcaccggc ccacagcatg gcaccctgac attgaacccc gatggcagct ttacctactc
      661 accggatatc gactggtcag gcatcgattc gtttagatat aaatcgaatg atgggttgga
      721 ggattccaat actgccactg taacaatcac ggtggtatct gcgattgaat ggaagttcat
      781 ctatttgccg ttggttttca aatgaagtca atttgctggg tcgaatcaac ccaaaggtgc
      841 caggaactga ataagaattc cagatgacct gatgaccaga aaatagttca gacatcaggg
      901 tttaagaata acttcaaata gggctaccag agaaaaccac accagagaaa aacattccat
      961 caaaagaaaa ctttaagatt tctccacttg cttttttgct tcatgtattg taaactgata
     1021 gcaactaatt taaccgcttc accagtctgt ctcgttgtaa ttacttcaat tggtctcgac
     1081 acccgtcgct cccgctgaaa aaatacgact aacctggaat cccttccagg ttagtcgttt
     1141 aaataccaac ctcttacctg gcttgaactt ttgaatttcc tgcgctatag ttatttcctg
     1201 tcacaatcta ttgtagagaa attcattcca tgagcagcag cctaaccctg acccaattac
     1261 acacccttta atggcagttt caactaacct ggcgattggc cgcggaagcg cagttgttca
     1321 gcctgaccga tgccgcttgc ctctggcagc cagcacccgg tagttggact gttcaccttt
     1381 ctgaagatgg aaattggcgc ccggattgga gcatgcccga gcccaaaccc gcccctgcca
     1441 cgaccattgg ttggcttacc tggcatctca tctggtggtg gtctggtctg attgccgcaa
     1501 tccgcgggga agaaccacca gaccacgccg cggttttctg gcccggcagt gccgaggcag
     1561 tcatcttgca gctaaaattg ctgtccgatc agtgggctga tctcttatcc caatttcagg
     1621 aagcagattt agagcgacca ctggcttatc cctgggcaga gccgcgccca ctgcggattg
     1681 ctctggcttg ggccaatcta gagctgatga aaaacattgc tgagattggg tgtatctacc
     1741 gaatgtttga agcagcccgc tcagcagaat aagcctgaag gcattatttt aataggcgcg
     1801 caggtatgga ttaggcaccg gtacaggctg ttttaaggtc tgagcagctc gcagcgccca
     1861 atgtggatca cgcagcattt cgcgccccag cagcaccaaa tctgcccgcc cattacggat
     1921 gatctcatct gcatgttcag gctctgtaat cagccccacc gttgcagtgc gcattccggt
     1981 tgcccgcaca gcctcgctca gcggtacttg atagcccggc cccagcggca tctgtgcttt
     2041 gggaatattg ccccccgaag agcagtccac cagatcaacc ccggcattga ttaattcccg
     2101 cgccagttca accgaatccg ttaccgtcca gccgccttca acccattctg ttgctgaaat
     2161 tcgcacggtg atgggcaggt gttcaggcca tacgagttga atctgcttta ttgtttcaat
     2221 cagaaatcga atgcggttct caaagctccc cccatatgca tctgcgcgct gattggacag
     2281 cggtgaaaga aaattgtgga tcagataacc atgggcggca tggatttcaa gccattcaaa
     2341 tcctgcctct gccgcacgtt gtgccccggc tttgaaggcc tgttgaacaa tccgaatctc
     2401 atctaaggct aattcatggg gcgtctgata tccctccgaa aaaggaatgg ggcttgcgcc
     2461 tacaaccggc cagaaatcag gttgtactgc cgtaaccggc ttgccgccat cccagggacg
     2521 ctgcgtgttg gccttccgtc cggcatgtgc aatctgaata cccggaaccg ccccctgcgc
     2581 cttgatgaaa tctgtaaccc gccgcagtgg ctcaatgtgc gcctcatccc agatgcccac
     2641 gtcatgcggt gtaatccgcc cgcgcgcctc taccgctgtg gcttctgaga taatcaaccc
     2701 cgcaccgcca gctgcccgag ctcccaggtg tgtcaattgc caatcatttg agtagccgtc
     2761 catgtagctg tactggcaca tgggtgcaac accaatgcga ttgcgtagag taatgcttcg
     2821 aatagtgagt ggctcaaata aatttggcat cttgactgtc ctgtctgcct ttatggcgat
     2881 aaaatggctt gttgatttag aattatagca ggtcaatttt cggtcagccc tgcccggcgg
     2941 cccacccagc cgctgcgccg ctggaaaagg caaattgcaa attgaatccg ccgcacgggc
     3001 caatgacgtc cagcatctct cctgccaggt acaatcccgg aaccagcttg gattccaggc
     3061 tggcagggcg cacctcatca actggaaccc cgcccgtgga gacttgcgca tagtcaaagc
     3121 cgcgtacgcc ctgaatttca accaatatgt gggttaactg cttcaaaata ttctccaccg
     3181 cggcttcagt caattgattg atttgcactt cggcattaat gcccgccaat cccaggatca
     3241 caggggggac cttaggcggc aagaccgcgc ctaacaagac gcgcaccggc accgcctctt
     3301 gccgtttttg tttaagcagc gctcgcagcc ggggttcact ttcccccggc agcaagttga
     3361 gggttaaatg cagcggcgtt ccaggtcggg tgctgatcag gtgactcaaa tccattaccg
     3421 ccggtccatt gaatccccac tgcgtaaaaa tcaggttgcc cgtggttgtg ccaagcagcg
     3481 ttttcccctg ccatacaccg gcggtgacat ccaggcgcac cccttgcagc ttgtgcagca
     3541 ttcgcacatc ggcttgcaca ggtgccagcg ccggatagac cggggtaatg gtgtgcccca
     3601 gccgagataa cagcgggtac aggtcgcccc gtgaacccag ggtggggtag gcctgtccgc
     3661 ctgctgctac gataaggcgc tcgccaatcc actcgctctc gccagcctgc acccgaaacc
     3721 cggcatctgt tttttgcaat cccgtcacgc gttgattgag cctcaaccgc actcccgcct
     3781 gggccagcgc tgccgcaaat gcatccacca ccgttgccgc cgattcggag cacgggtaat
     3841 accatccatc tgccgtgtga taggctaata tgccaatccg ttccagtgtt tttagcagtg
     3901 cttcccgatc aaactggttg aacaaggccg ccaaaaacgc cggattcgag caaacatacc
     3961 gctctggtgc aatggcggcg ttcgtcaggt tgcagcgccc gctgcctgtc accagcagct
     4021 tacgccccac aatggcattg gcatcaaata atgtcacctc tgcgcccttc caggcggctt
     4081 gaagtgctgc catcatgccg gcgggcccgg ctccaataat cccaacctgt gttttctttg
     4141 ccataacctg accttaacgg tgtttttacg aaattttctg aattgtaacc gatagcattg
     4201 gctttcagcg cagatacttg acgtaaacca attccgggtc gccctcatcc aaatcatgga
     4261 tcacgccgct taaccgataa ttaaactttg ccagcagtct ctgcattggc aaattggaga
     4321 gattggtgga tgtgaacagt ttatgtgtct ggcagacctt ctccagatac cccaacagta
     4381 tttgaccaac accctgccgc cgccagtctt gctgtacata cagcattgca ataaacccct
     4441 gtgcaaaaaa tgaatagttc agtaccgcat aaccaacaat ttgcggcctg gcggccacat
     4501 aacagatctt ctctgccacc gcccgctgaa taaactcccg gcgttcgttg ttgtggcggg
     4561 caatttcgtc cagcgcgcag attgcctcaa tctgatcttc ggtccccagt tgaacggtaa
     4621 tcatttctac tcgccattct ccaccggcaa aattgcctct cccagcactg cggtattttc
     4681 tgcaaccagc tgctgaaata cgcttgctaa ttgcggattg tcctgtaccc gcatgttggt
     4741 gtcgtgcgcc gggttgtcgt cattttgcca ggtctcattc caccaggaga agccaatcac
     4801 ccgcggccag cgctgctcgg tcagatcgct caatgcatcc tctgcccatt cggcctggtc
     4861 tcccagtggg ttattcgccg tcacgccaaa ttcaatcaag ataatgggct tgtccggtgc
     4921 cagtgccgtc aggcggggat aaaccgcatc catctgtgag cgcagatccg gccattcctc
     4981 gtccagcggc gtttgcgccc cgtaaacact gaccccaatc caatcaaccc acgcgtcgcc
     5041 gggataataa ttttccaggt ggttccaatc gtcttctggc caatcgctcc cattaacatg
     5101 gaaaacccat aagatattaa tggcgccctg ttcccggctg atgtcgatga tatgccgata
     5161 cgcgtctcga aaccgctcag gtccatcagg ctcattgggg tcaccatagc ctgtcagatc
     5221 cccgccgccg ttccaaaccc cgttccatga gaaccattcg ccgttcattt ccgtcccaaa
     5281 ctcagcaata atcggcgtcc caaaatcccg cgcagcatca gcccagtttc tcaggtctgc
     5341 atcaaaatca ccgttgatga tccgctccaa tgtgaacacc ggttcgccat ggtcaagcga
     5401 ggcgtcgctc cgcatcatca accgcacata gggaatactc cccgccgtcc gaatccattc
     5461 cgcagtcgcc gtgggaaatt cgcgcccatg ataccagttg tgtgaaaaga acacccaaac
     5521 cgccggtttc cccaccgctt cctcgtagga acgcaggtct tgcagcgtca gatcatcctc
     5581 ttcccccgtg attccccccg gatacacgcc atgatatagt tgcccctctc cgggcaatcc
     5641 aatcagcgat tcagactccg gtgtgttccc ggtttccacc ggcaccgtct ggcatccggt
     5701 taacagcgca gttattagag caatcaacag gcagcttttt aatctcatat gagtatcacc
     5761 tgattgtgga agtaactcta attatacttg ctgccgatgc ttgacgatcc cctgtataat
     5821 ggtaataagt tcatccatac aaggaatttt cattcaatgg aagaattgac cacaaaacct
     5881 gcccctgctc gcccaacaat ttatatcatt tctggcggtg tgggggctag cgctgagcag
     5941 ttggtctaca ccgttctcgc gcaatatccc gagggtgcgg tagatgtctt caccaccggc
     6001 aacgtgcgca cctttgaaca aattctaccc gtgctggagc aagcagccac caagtctgct
     6061 ttcatcattc ataccctggt cgacccctca ctgcgccagc agctcattgt ggcagccgca
     6121 gagaggcaaa tccccgcagt cgatttgatg ggcgacctga tgggctggct aacgcagcaa
     6181 ctgggggaac tgccctatat ccagccgggg cgctaccgcc gcctgcatcg agattactat
     6241 gaccgcgtgg ctgcgattga ttacactctg gcgcatgatg atggcaagca ccccgctggc
     6301 tggcgcaatg ccgaaatcgt tttgctgggt gtttcgcgct ctggcaagac ccccatcagc
     6361 ctttatctgg cagtcctcgg ttggaaggtc gccaatattc ccctcgtccc gcgcctcgat
     6421 atcgctgacc aggtctatac ccttgaccct gctcgttgta ttggcctgat cctggatgcc
     6481 gaccgtcttc tgctttaccg ccgccagcgt caggctcgtc tgggcgtttc tgccgcatct
     6541 gattacaccg atattgacga aatcgagtta gaattgcgct ctgccatgaa gctttaccgg
     6601 cgctgtggct ttcaggttgt ggataccacc gacaagacca ttgaaatgtc tgccgatgaa
     6661 attctacgcc gcatccaaaa caaaccgcct aacgctttgc cggtctaatg gcggtttctc
     6721 caaccagctt attttagcgg ttgagggtct ttcatctgca agatcagcac tgtgctgata
     6781 accagcagtc caatggatac cagcagcgcc ggtgtgaatg acccgtccgg cgttttaagt
     6841 gcttccatga tgtaaacaaa caccactgag gcctgcccaa acaattggat taacccgttg
     6901 gaggtgcctt ccggggtggg gtgggtcact tcagccgcat actgcatccc aaccgggctt
     6961 gcgctaatca ggaagaaccc cattgcaaaa gccgaaataa acagcagcca tgccgagctg
     7021 gcaaatgcca gccccaccag acccggaatc gccagcacaa atcccatcaa gatgtagcgc
     7081 cgccgccggt gctgccgatc tgagaacggc ggaattaaca ccgctcccag cacgccgccc
     7141 accagcatca gcgcgcccag cgttcccgca tctgtgggag tgaaccctcg tggccggatg
     7201 atattttcca cccaggtggt tatcccatta aagaccccca tcccgataaa cgagacaaac
     7261 agataaagcc agaactcttt gaccgttaat gcatgtttca acccatccag catcaaggcc
     7321 cgtacctcca gacctgccgg gcagggtggg gtggcaggct tttcgcgtgc caggatcaga
     7381 aacaacaccg ctgaaaaggc cgccacaccc ccataaataa gttgaacatg ggggatggat
     7441 aggctctcag tcaataatgg cgtcagcacc atgcccagcg ccgtgccaac caggttggat
     7501 aatgtaatca gtccaaccgc tgtggctcgt tcttcaatac tgaaccattg tgccggtact
     7561 tttgtccagg cattcaacaa gaaaggctgc gcaatggcga tcccaatcgt gctcaataac
     7621 accagggtat aattgctccc cgccagtcca cgcaccaccc caaagatgcc catcaaaacc
     7681 gccccaatgc tgaccgtggg ccggaatcca taagtatcaa ttgcccacga gaccggaatg
     7741 gatagcggta tgaaagccat cataaaggtc atcgccagca gcccaatctg caaatccgag
     7801 acgccataaa attctgcggc caccccggta atgggcgcat aagcgatcca caagatctgg
     7861 attgtcaaat tgatgaacat aaataccgcc aggacaaccc agcggtaacc atacaggcgg
     7921 aatgattttt cggtcatgcg gttctcctca acggttcaac tggtgataaa tgcccttcat
     7981 ctgccggtaa atgcgggtga aaatggcaaa tcgttcatca taaatggaag tattggcttg
     8041 ctgtggttca tagaggcgct taaattgcac caatccgggg atatcattaa atccaatttc
     8101 tcccagtccc acggcgccaa tccaggccgc gccacaggca tttgcataaa caggcccggc
     8161 cacctgttta atttccacat tcagtgcatc ggcaaaaatc tggcaccaga tgtcggattg
     8221 tgcccctccg cccacaatgt gaatactgcg cgttttccgt cccataaacc gatccatggg
     8281 ttccagcacc cagcgtgtgt tcaaagcaat cccctctaaa aacgcccgaa taatatcttc
     8341 gcgggtgttt cgcaatgaca gattgtacag ccctgcccgc agtgccttat cctccaccgg
     8401 agcgcgctcc ccccagatcc acggggtata gatgacgcca ttggcgccgg ccggcacccg
     8461 ggcggcgatc tggtccaaca ctttgaagat gtccggcacc tgcgcctctt gcagcagttc
     8521 atccttgtta tagatgatat tatcccgcag gtaagtcaaa tttcccccgg cagtcgcctg
     8581 cagggctgtc aacaggtagc gtccgggcac agcacacggc accgaggcca gtgaagcaaa
     8641 tacatcggtc tttttatagg gcacatgggc cgccagccag gaggatgtgc caatgtacag
     8701 atgcggtatg taatcttcaa tcgcccctgc tccgatggcc gccgcagtat tgtcgattgc
     8761 ccccgctacc actttaactt cgctgctcag tccaagctgg gctgccacgc ttggcaccag
     8821 gggacccaac acggcggtgc agggcacaat ctcaggcagt ttatctcctg ccacgccgca
     8881 agaccgcacc aacccactgt catagtggat attatccggg ttccgattat cggtcaccca
     8941 tgatgtcagg attgaatcaa ccgtggctgt aaaccgcccg gtcaactgca aatttaagaa
     9001 atccagcaca ttcaaaaatt tataggtctg ggcgtaaact tcgggatacc gatcccgaat
     9061 caagagcatg tgcgccgccg ggtctttgcc ggtcgccgag ggcatcccgc cggtcaaacc
     9121 gatccaccgc agcacatgct gtgcacccaa ccccgaaacc ttgataacgc cgccaaattg
     9181 ccgtctcagg tttgctgcgc cgcgcatatc catccacagc acacaattca tcaaggcctt
     9241 gccagcgcgg ttcaccggga tggtgccctc gccctgcgtg gaacaacaca ccgccttgat
     9301 ccggctcggc gcagccaacc cgcgcccaat caagcgttgg gcagctgtga tgaacgcccc
     9361 ccaccattca tcgggcgatt gctctgctcc gccatccggc gtcagataaa gctgaatagg
     9421 ctcagagtcc cacccaatca ccttgccatg gatggtgatc agcgccacct tcatgccaga
     9481 agtgcccaga tcgatggata gaatgatggg ggcgtttggg tcgctcaacg ttaatctcct
     9541 gtctggaagc taggcgcctt caatatttga aaataccgct tcaaaccgcg ccagcgcttc
     9601 gtcaatcacc tcgtctgtat ccgccatgct ggtgtacatc cggctgcctg ccagtgtaat
     9661 cagtccattc gccaggtagg ctgcccccat ctcttccagc atgtgcttgc gcttcttcaa
     9721 ttctttgcta agccggatta gattgcgcag gtccagcagc atcacaccag aagtttccag
     9781 atggcaaatg gatccctggt tgaaggccac aaacggcaga ttatattttt caatcagctt
     9841 ctgcagaccc gcggtcaggc ggtcgcccgc acgtccggca atcaccggcg cattggtgcg
     9901 ctccatttcc agcagcgcat gataccccgc cacgcacgat ataggattag ccgacagcgt
     9961 ccctccaatg taagcattct gccccgttcc gccgatgcct gccgcaaatc gcatgataac
    10021 ctctgcgcgt ccacccacac cgcccgccat cgggtagccg cccgtcaggc acttgccgaa
    10081 tacggtcagg tctggtttca cgccgaagta accttgcgcg ccgcccagcc ccagccggaa
    10141 acccgtcacc acttcgtcaa aaataagcag cgccccaaat tcatcacaca gctcgcgcac
    10201 cttctgattg aaatcatagg gcaccgggcg tgtgccgctt tcgggcccaa caggctctac
    10261 gatgactgct gccgtaccgc ccaacactcg attgaggatc aattgccgct tcaatgcgcc
    10321 cagcgcattg gggaaaaact cttgcgtgtt ggctgttgcc cctccaggaa tccccatggc
    10381 ctccagccgc cctgttcccg ggatgtgcaa cccatagacc atcgagtcgc tccagccatg
    10441 gtaggccccg ccaactttaa tgaccttttt cttcttcgta aaagcccgcg ctgctcgaat
    10501 ggcagccatc accccttcgg tgcccgagcc caacatccgg aacatttcaa tattgggcat
    10561 caagcgctgg atgagctgcg ccagtttcaa ctcgtattca tggaacaatc ctgtcaccgg
    10621 cccacaggat tgcagcattt caatgacttt ggcgcgcacc ggttcgtaat tgctacctaa
    10681 aattgtcggc ccaccggctt gcagaaaatc aatgtaccgg tttccgtcca catcccataa
    10741 atgggcgcca tccacctttt caattgcaat cgggaagggg tagttaaatg ccagattgtg
    10801 ctgcacccct ccgggaatta acttcttggc ctcatccgtc aaagccttcg aacgagcgca
    10861 ccgggtctga aaatatccca ggtattcttc catcttctcc cgccgcaccg ggcggatggg
    10921 ctgggccacc agcgcattca accggcggta cacatcctct acatcaggat atttagaaat
    10981 ggcataggct tgattttcag tcattttgtc ctccagtttg ctctttagtt tggggttcac
    11041 tcgccgccag tttgggtgtt gccgctctcc acccgctttt gatctttgcg cagcttggca
    11101 aacgccagtt ctcgtatcgg cagagggatt ttgctcactt tcttctccga aagcaccgcc
    11161 agcaggtaag ccaacgcaca cttctccagc acttccacat tatgcatcgc gcgctccagg
    11221 tcgtgcccaa acagcagtgc cccatgattg gccatgatgt atgcattatt gtgattgctc
    11281 acatgccgcg ccaccgtatt tttcaacatc cccgttcccg atggagcata agggattaca
    11341 tccaccgagc gacccaaaaa gcgcacctgt tcgtcaaaca atgccggaat gggcaggttg
    11401 aggattgcca gcgtgctggg gtacacctga tgcgtgtgaa taatggcatt cacatcgccg
    11461 cgtgcctggt aaatggcgcc gtgcattccg ctctcaaccg atggcttgcg ttccccggct
    11521 aagggatgca agtcaaagtc caatacgcag atatcttcag gaaccatttt catgtagtca
    11581 tagttggaag gggtgattgc aaaagccttc tgccctgcaa ttctcaccga taaattgccc
    11641 cccgtggcgc tcaagaaacc tttgcgggtc agtaattgcg ccgcgttcac aacctgtatt
    11701 ttggcatcct caaacatggt catggtaaaa acctcacttt gaaatcttta cattgtcaag
    11761 attgtagtat aaaatctaga cagcgtcaag attttgcccg aggtattttg atgacccaca
    11821 aacaatacca tcatggtgat ttgaaaaatg cattgattaa agccggaact gagatcctgt
    11881 ccgaagaggg ggttgcaggg ctttccctgc gcaaggtagc tgcgcgcgcc ggcgtcagcc
    11941 attctgcgcc ctatgcccac ttttccgaca agcaggcttt gattgccgcc atctctaccg
    12001 aggggtttcg gcagctcaat gcccgtctga gcagcatttc agatgcctgc cagaccgaac
    12061 ccgcccgcct gctgctcgaa gtggccttcg cctacgtcca atttgcgctc gattcttctg
    12121 cctgcttcaa gctcatgttt tctggcgtac ttgaaaagga aaaagactat cccgattttg
    12181 tcgcaatctc aaaagccagc tttcaactgt tggtcaccct tgtgcagcgc tgtcagcaag
    12241 tcggattaat ccggcccggt gatcccgatg ttctggcggt cagcatctgg agcctggttc
    12301 acggctttac cgccctgctc ctcgagcgtc aaatctccag ccacatcctc gaccgcttca
    12361 ccatcaaagc gctgctctgc cagacgctca atcaggtcgt cctgactgag atccagccag
    12421 tagaaacgga ttgattaaac cccatttctg atcagccgcc gtaaaatctc caggatctga
    12481 tatcgccctg cgtccatccg cgccagcata gatttatctg ccagttgatc cagttcgttg
    12541 gtcaataatg ggtccaacgc aaacacatcc gcaaaagtaa attcaggctc cagcgtggca
    12601 cagagcttta ataccgcctg ctccctttca gacagcagcg cccacgagct ttcgaacacc
    12661 tctcgcatac tgctgtgcct ggatggaata tcactgatca gtgatgccgg aaatacaatg
    12721 gggtcttcct caatgttttc attaatctgt gccagggtca ttcgggttgc ccagggcgct
    12781 gccagctcaa tagccagagg atttccatta agcttattgc agatctggat gatttgccgc
    12841 atatcttccg ggctgccggc atcaaattca cagcccgcgc tgcaggctct ttcaataaac
    12901 aactgcactg ccggtgcgtt ggcggcctct gcctcgtttt cttcgggcac caccagcccc
    12961 tccagtcgca taatataccc ggcttgaaaa tagagcgcct gccgggtggt aatcaccacc
    13021 cgcacctggg gagcctggtt taacagctca aggataatgt cggccccatc cgaaaaatac
    13081 tcaaagctgt ccaatataat cagccgtttt gccgatccaa attgactgaa gagcctgtct
    13141 gctggcatat caggcgtgcc agataaaaac ggcagatact caatcagcat ctgtttaatg
    13201 gtgtgatgaa tttcagcccg cgttgtagaa gcctgcttat gggtttctaa tggcagctgc
    13261 cagatgcccc cctcaaactg atctatcagc gtttcggcca ctgcctgtac aagccgcgtc
    13321 tttccaaccc cgccttcgcc aaccagcgta acccagcggt atgcaaagat caaccggctt
    13381 aatttgtctg cctgtgcctc gcgcccgatg aatgatgtca gcgcagcacg caaatttccc
    13441 cggctggagg gtttgctggt ttcctgcacc cccagcccgc gtttgctggt gattttttta
    13501 taaagctggg tggtttcaac tcccggctcc atctcaaatt catcaaccaa aacctggcag
    13561 caaatccgat actgtgcaat cgctgccgga acctgccctg tcatagccag cgcgctcata
    13621 attgtctggt ggcaggtttc ctgccagggc tccagcagca aggctcgccg tgaatattgg
    13681 atggcctttt caaaagctgc ctggcgtcca ggcgcggtaa tggcgtcaat cgcaagcttc
    13741 agatgaaacc gacccagatc tgtcagcgcc tcaagcgtct ggcgatgaaa ccgctctcgc
    13801 tgcacaacca tccattcttc aaaactgctg gatgaactaa catccacgtt cctcagaaac
    13861 gcatcgcgat acaaatcaac ggcctcggcc aaacgctgcg cacattctga actcaccccc
    13921 ccattttcgt gagaatggtc ggcacattcc gtcatcaaat cctggaattt caatgcatcc
    13981 acccaaacca tcagatccgg gtccaattga atggtggcgc ggtccgttcg gaatatttta
    14041 acttcctctt cagcccgcag ggcaacttcc agcgttcgct tcaaccggtg cagtgtcaac
    14101 cgtaagttac gtaacgcatt ctcggttggc acatctggcc aaaacatttc tgccagcacc
    14161 tcgcgcgaat gtcgctgccc gcgtcccatc gccagataag ccagcaaggc ccgcgcctta
    14221 tttgtatcaa agcgccggat cggcacatcg ttccagataa ctctaaattc tcccagcagg
    14281 tgtatataaa ggattctccc catctcacct cttttgtgac cgtttcataa ttataccctg
    14341 tttgcagaaa aactgaaacg caaatgaaac gcaattgaaa ttcatccggc gtattctata
    14401 accatgcaag gttcaggcca gaatcccgtt aaaaaacgta actacgcctc gtatctgtta
    14461 cggtgctggc gcgtacagga cactgctgct tggaggtttg tattagaaga agttatgggg
    14521 tccgaaaaaa taaattttga tagtctgtct gcattgatcg cctacctgga gcaaaaattt
    14581 cagaccggcg attcaaaatc ttatccacca tccgaaaacc aatctacaag gagggaagaa
    14641 tgaataatcc aaaacctggt tccaccatgt cgaacaccgt cttgaaacga atggtccaga
    14701 tgatgttcgc cgccattgtt gtggtcgtgt tcgtgggcgt tgccatgttc accctgccac
    14761 gacagtcaac cgccagcggc tccaatcagc tcggcaacgc cgtaccggct gctgccctca
    14821 tcagcgacgc ccagccgcag gctcaaccca cccccaattc ccaaaaagaa gtcgtcccgc
    14881 ccgattatgc ccctggcggc ggcttttacc cacccgtagt tgatcagggt ggtgattgca
    14941 tttccggcta cgttattgat gtttaccatc agaatgccgg cgctggctgg gaaattgtcg
    15001 tcacatccaa agaaaccggc caggtctgga cccaaatcac cggcgttgat ggccattaca
    15061 gctttagtcc actgccggct ggtacctatg tcgttgaaat caagtatcgc gagggctggc
    15121 gagccttcac gcttcaatcg tttgaagtca ccttgagcgg cgaacccggc gtttgcgcag
    15181 tcactcgctt caaaatggaa gccctgccct gcctgattgt tcgtaaaatt gataaaaaca
    15241 gcgctggcct acccaacccg gtgggcctgg ccgattggcc cattaccgtg cgcaacggcg
    15301 actactctga aacaaagctc accgattggg atggctatgc tgaatttcgc tatctggtcc
    15361 ccggaacctg gattgtcgaa gaagccaacc agattggctg gatccccgaa gtgggccagc
    15421 cctccagcat tacattatct tcaccccgcc aaccttatac ctgtgagcgg attgttctgg
    15481 tcaataaaca actccacaca tcctgcattg ccgccaagaa ggtagatgat cagggccgcc
    15541 cgctggcaaa ctgggtgatc aacctggtac gcaaagacgg cacccgcccg cctcgccaaa
    15601 ccgtcaccga tgccaacggc tatgcattct tccttgacct ggaactgggc gaatggattg
    15661 tatccgaggt ggttcaggat ggctggcagc cagtcggcga gacctcctat acagtcaatc
    15721 tggatgtacc aaccacgctt tgccccacgg tcaccttcat caaccagccc actggctgta
    15781 ttgaaggctt gaagatcaac cactacgatc aaggactgcc caactggcgt attaatgcca
    15841 cccatacggc caccggtcag cagctttcaa ccatcacaga tatcaatggc tacttcaagt
    15901 ttgaaaacat gctgctgggt gaatgggtaa tccgtgagga aatgcagccc ggttgggaag
    15961 ccgtaacccc cggtgaattg accgtgaccc tgacagatcc cttcaagtgc gaacagatcc
    16021 gcttcaagaa catgaccgag ttcgcctgtc tggatgtgta caaatacgat gataccggcg
    16081 ctccgctccc cggctggacg atgatcctgg aacccgctta tggcggtgag cagctaaccg
    16141 gcattacaga tggctcaggc tgggtgcgct tcaacaaact gactcctgga gaatattatg
    16201 tgactgaaat cgtccaggag ggttgggacc ccatcacacc aatccatcaa tatgtccggg
    16261 ttgaagccac cggcacctgc cgcatcatgg aattctacaa tgagcaggaa tcgccctatt
    16321 gcgatgatga ttgccatgac gactgtgacg acgactgtga cgatgattgc tccattgacg
    16381 gctgctgcaa tgactgcaac aatggctgct gcgatggctg caacaatggc tgctgtaacg
    16441 gctgtaataa tggctgctgc accaattgca acaatggctg ctgtaacggc tgttgcgact
    16501 ccacgtactg caccgcctcc tccagtgagc aaatttggaa tcccccggat cccnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctccacg tactgcaccg cctcctccag
    16681 tgagcaaatt tggaatcccc cggatcccag caattgctgt tgttgctgtt gctgcggtgg
    16741 cctaacaggc tgcaccccgg attactgccc cacgggctgt gaccagaatt gtagtgcggc
    16801 tgcctgcagc tctgcaacag atgtatcgat catatatccg aacaactgtt gctgttgctg
    16861 ctgcaccccg ccctacatgc cctccctgcc ctctgacggc agctctgatg gcgacagcag
    16921 cgggacaacc acctatgttg tcaagtctgg cgataccctc ttcgccatct ctcaagccta
    16981 cggcatcacc gtgaatcaat tgaaggccgc caatggcttg accagcagtc tcatctacac
    17041 agggcaaatc ctcaaaattc cctgatcccc tacccatagc aaatcaaaca actcctggcc
    17101 cccaggagtt gtttgattct caatcatttt ttaccacccc tcataatgca ctcggtctgg
    17161 atcaaaccgc tctgccacgg tccgatcctc aattttatgc cccaccgcaa tcaatcccac
    17221 aggcacaata ttgaccggca gcttcaaaag cttgcccatc ggtttggtga agattgagac
    17281 cggatacaat ccacaccaca ccgcccccag ccccaatccg tgggccgcca gcagcatatt
    17341 ttggatcgcc gccgagcagt cttccactag aaagcccacc tgtggctgtc tcagcttatc
    17401 gccgcaaacc acaatcccac agccagcctt gggcagcatc tgggtgtaag gctgcacctt
    17461 tgtaatcgct tccagcattt ccggctcgcg cagcaccaca aaatgccagg gctgccggtt
    17521 catcgccgaa ggcgcgtaac tgccggcccg caggatggcg gtcaaatctt ctgcgccgat
    17581 tggctcgcca gtgaacttgc gaatactgcg gcgggttaac aaagcttcca tcaattccat
    17641 cggttcttct cctggttcgg tagtttcgag cttgcagtat aatcaacata gcattctact
    17701 ttggaggttc tgcaatgtca ttttgggata ttttactttg gattgtcatt ggcggcctgg
    17761 ctggcctgct ggctgattgg gttgtcaagg gcattcgggt gggcttgctg ggcgccatca
    17821 ttgtgggcat tctgggaggc tttttgggcg gttggctgtt tggccagctt aatatcgcca
    17881 ttggttcggg gttctggggc agtctgatct ctgcctttgt cggtgccgta attctgttgt
    17941 ttgtattgcg ggccctgcgc cgcgtcttcc gcactcgccg ccgccgccgc taaccctcac
    18001 cactcaattc cacacaactc gcaagagacc tcccacaact cacgggccgc tttttctgat
    18061 cttgctgccc ggctgggctg tttgggcgcg ccgttatacc agtagggcgc atcggtcaga
    18121 tcttcagcac aggccaggtg cgcaatggtc tgggcgggca cttcagccgg ggtgccgcct
    18181 ctgcgccgca tctcccaaat ccagcgtgag agtagcccgg ttcctttgag gccaatctca
    18241 gtatttacca gtcccgggtc caccgcatag gcccgggtgg gcgtcacgcg ccggttaaac
    18301 tcaatcgtaa acagtacatt ttcaagcttg gatactttgt atgcccacag cacatggtag
    18361 atcaatggat ggttgatccg ctttggattg atccaggtac gataatgtga ttctgaactg
    18421 gtggtaatca cccgccctgg ccttgccgcc tgcagccacg gcagcagttg gtgggtgagc
    18481 agaaacggcg ccaggtgatt taccgccagg gtgcgctcaa tgccttctga tgttcgctgc
    18541 aaggaactgg caatcatccc cgcattgttg accaacacgt ccaaccccgg cacgccccac
    18601 cgcgtcagtg tctcgccaat ctcagtcgcc agccgccgca cctcgctttg tagcgccaaa
    18661 tccgcctgga gaaactgcac cacagcgttc gggttcgcct gttggatcac cttccccgct
    18721 gcctcgcagc gtaccgggtc tcgccccacg ccgatgaggc gcgcgccctg ctcggccaat
    18781 gcgcaagccg ccgccagccc aattccggcg gttgcaccgg taatcactac cgtcttaccc
//
LOCUS       DAOPVS010000027        18271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3104_length_18271_cov_55.814449, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000027 DAOPVS010000000
VERSION     DAOPVS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18271
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3104_length_18271_cov_55.814449"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..964
                     /locus_tag="PKL11_04175"
     CDS             161..964
                     /locus_tag="PKL11_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12832.1"
                     /translation="MKVPPRYNEHIPMNLTYPPELFDLNFAFVYRVSEILEQPWPDLL
                     LNYTHTFRRFRLGAVLDPQNPIWLQFLEGVRRADEPARYAYAFYLQREHDTGPLQRQN
                     AFGCFSYTLEKADCDTILRIHFANQSPAGTGPLSHENQALRRTELTEMFIDARQVVKQ
                     PCLVRGASWLYNLSGYRRLFPAEFIRSLVARPPDFPMLALWGQFLDHNWQIRPDLAGV
                     FLERIGRAQSMTDLEQAFPYSILIACAGIEAFWAFYDIGTACQNPALKS"
     gene            1142..3922
                     /locus_tag="PKL11_04180"
     CDS             1142..3922
                     /locus_tag="PKL11_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ12833.1"
                     /translation="MKFMFLGGADEVGASSTLIEIDGKRILVDCGIRPSPKARDGLAG
                     DQLPNLDQITRPDAILVTHAHTDHTGALELVTDRYPDVPVYATAPTIALIRVLHQDAR
                     RIMQSRLDEEGELPLFDDVSVQRLMAALVPVPFLSRVPLGEGVSVTFFPAGHIAGAAM
                     LGLESSEGNLLITGDFSISPQRTVDGARPPAFHPDVLVVESTYGGRLHANRHVQERKL
                     IETVAAVTGAGGKVLIPAFALGRAQEILLILSEGQRRGELPLVPVWADGMVRTICHAY
                     TGFADVLPLSLQERGARFFEGHIQPVQTAEQRNALIWQPEPAVIVASSGMLAGGPALL
                     YAKAFAGLGQHAILLTGYQDEESPGRRLQELAEKGHGTLRLGNEKVDVQCLLGTYSLS
                     AHADEGQVVSLIETLNPERVVLVHGDDPARASLEKALAERERLVDLPHAGQTLELQFE
                     RRRPGRGETSGIDFRDRQLTLTRERQIKMGNFTGCVGKWLLLKGSVPTPVRCLALEND
                     HLWVEITPGLEQSVYPEEILSVLGDTPPAPADVTFAQSASASPVVMEPNQAMAAANQR
                     FPAAARLRKVGYHLTEQSLVLTFDFPGVASRQFAETIADLQKATGWEVVVTPEANQGA
                     LNAFLREVLPEDWQMTKSPAIHREQQRVVAAVRAPQSSQVDAVESICSRYLAETGYVL
                     ELVVSQAGAPADSPVVLPVAWRGERLEINAAYTLIKSSLEGSTLYRTSLKGETIMLSF
                     ISPQVGERYRQQIEDLSVQIGWPLTMNLQPNQGAILEIARALMNAVHWQVVKGPGVYP
                     EKAEVIVSLGNAPAETEISELAASFEDQTGYRLVVFAARPALADQKSPALDGQVIEIS
                     LAQIRLGHSHAAISLDPLKLEKAIERARRMGITPPIQVRRTRTDYVLVDGLYRLRAAQ
                     ALNLEKVPAIVE"
     gene            complement(4163..5761)
                     /gene="nadB"
                     /locus_tag="PKL11_04185"
     CDS             complement(4163..5761)
                     /gene="nadB"
                     /locus_tag="PKL11_04185"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNZ12834.1"
                     /translation="MKITDVLVIGGGIAGACAALRLARDPNRRIILITRETDPHESNS
                     NYAQGGIIHRGEADTPELLAADIINAGAGATNPAAAAILAGEGPELLEDLLIHEGGVH
                     FDHSPDGSLAYTKEAAHSCRRILHVGDGTGKAIIQGLMAAVQNRPNIELLTRATAVDL
                     ITYPHHSRNPLDTYGPVTCYGAYVFDQNGRAIHRTLAAKTILATGGVGRIYRNTTNPP
                     GARGDGLAMAHRAGARIINAEYVQFHPTALAVPGAEGFLISEAVRGEGGVLLTPEGVP
                     FMERYSPEWRDLAPRDVVARAIHTEMEAHGYSYVLLDIASHMAPEAIQARFPNIYATC
                     LKAGVDMSHEPIPVVPAAHYFCGGVAVDLWGRTDVRNLFAVGEVSCNGLHGANRLASA
                     SLLEGVVFGARAADQIAAELAEGAFVPQPVDIPIWDESGLTQDSDPALIQGDMQTIQN
                     IMWHYVGLIRSGERLSRAIRELRHLQNEIETFYRKTKLSDGLIGLWNAVEAALIIAKA
                     ARHNRQSRGGHYRVDSVTVGERLL"
     gene            complement(5771..6814)
                     /gene="nadA"
                     /locus_tag="PKL11_04190"
     CDS             complement(5771..6814)
                     /gene="nadA"
                     /locus_tag="PKL11_04190"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNZ12835.1"
                     /translation="MNSEAQVLYEEMKTRLGQFVPDFELRYKAEIAVEINALKKERNA
                     VILGHNYMEPVLFYTIPDFRGDSLDLSRKAAQTDKDVIVFCGVKFMAETAKILSPTKT
                     VLLPAERAGCSLAESITAEDVRALKRKYPGVPVVTYVNTYADVKAESDICCTSGNAVA
                     VVESLNSDTVIFLPDQYLAGNVARETGKHIIFPSRLGTTPEAETLLDVQMIGWTGRCE
                     VHEKFTVADIQAVRAQFPDVAVLAHPECPPEVVAAADYSGSTNAMIKYVQQSNAPHYL
                     ILTECAMGDNIAAANPEKNLLRLCMVRCPHMNMITMENVRDALQHNRWVIEVPEEIRV
                     RAFQAVDRMIRIG"
     gene            complement(6839..7672)
                     /gene="nadC"
                     /locus_tag="PKL11_04195"
     CDS             complement(6839..7672)
                     /gene="nadC"
                     /locus_tag="PKL11_04195"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNZ12836.1"
                     /translation="MSIHPDILEAIQRALREDIGAGDATSNSIIPADAVMTGQIIAKQ
                     SGVIAGLDVAQAVFAALDPRVEFTALVEEGARVENRQVVSRLAGPAVSLLTGERTALN
                     FLGRVSGIATLTRQFVDAVAGTRAVILDTRKTVPGLRMLDKLAVARGGGQNHRIGLYD
                     MILIKDNHIDFAGGITAAVERARAAHTGLEIEVEARTLAHVQEALELGVERILLDNMS
                     LETMRQAVVLNVGRAKLEASGNVTLETVRAIAETGVDYISSGALTHSAKVFDVSLDFI
                     R"
     gene            complement(7794..9005)
                     /locus_tag="PKL11_04200"
     CDS             complement(7794..9005)
                     /locus_tag="PKL11_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ12837.1"
                     /translation="MRKILIPAIGTRSDVQPYLALGLGLQRAGHTVTIASHPGMGQWI
                     KSHGLTFASIGPNIHLGNEAEKIRTRAKNKVLGFISLMEFTASIVERCSKDILKLCPG
                     ADLMIVTHSFAGRAESDKAGIPFISVTIQPQIIPVVESHQTGINRATSAVIGAVMNSM
                     MVGPYNHIRKQLNLPPIKGSLENMMSGRLNLVPVSPLVVTPDPRWAPQHRMVGYWLLG
                     EPPGWSSPVEIRAFLDAGGPPVAIWLGGSLSGKETIDLLKMFVEATRFAGVRAIIVGS
                     QEAARKIDLPPTVMVSGWIPPSWLLPKCCALVHYAGFTITGAGLRAGIPAVSIPQTVD
                     QDFWAQRIYQLKCGPKAIPYPELSSERVALALIETLRDTQMRQQAQMLGEQIRLESSV
                     RLTAQMIEQAF"
     gene            complement(9119..9691)
                     /locus_tag="PKL11_04205"
     CDS             complement(9119..9691)
                     /locus_tag="PKL11_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNZ12838.1"
                     /translation="MDLITTLLIAVGVAMDAFAVSLGIGTSPNGRNMRAAFRLAFHFG
                     FFQGGMTLLGWLAGSTVERFISSVDHWIAFALLAFVGGRMLVEGLKNHTEEEVETGKD
                     PSRGGTLVILCVATSIDALAVGLSMALIEVKIAIACSVIALVTFGLSLVGVRLGQRLG
                     ARFGKRMEVLGGLILIGIGIRILVSHLMGL"
     gene            9828..10025
                     /locus_tag="PKL11_04210"
     CDS             9828..10025
                     /locus_tag="PKL11_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNZ12839.1"
                     /translation="MPNESMTDRIIRVVLGIALLALGWTGTVSGVLGEIFKYLGFVPL
                     LTGLIGFCPLYALFKFRTNKG"
     gene            10106..10177
                     /locus_tag="PKL11_04215"
     tRNA            10106..10177
                     /locus_tag="PKL11_04215"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10138..10140,aa:Gly,seq:tcc)
     assembly_gap    10179..10278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10515..11426)
                     /locus_tag="PKL11_04220"
     CDS             complement(10515..11426)
                     /locus_tag="PKL11_04220"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HNZ12840.1"
                     /translation="MSDPIKVAVMGCGHIGIYAARAAQLAADMHLIGAVELPARIEAV
                     RHELPGVTLATSLEALPEKPTVVILALPTRTVEKVAPTLLAQGIHTVDCFDMHGGPFL
                     QLKATLDPIARANQAAAVMGAGVDPGVSTLIRALFEIWAPTGLTYVTYGPGMSMGHTV
                     AAKSYAGVKDALSITRPGDPGCHKRDLYLELEPGADFESVKAAILQDSYFCHDDVRVF
                     QVDDVTPLVDMGHRIDVRRKGGASGVDNQLLHFETTFHNPASTAQVMVGCARACTRLS
                     AGAHTLLEIPPYMYLPEDLTGLVKRLV"
     gene            complement(11428..11886)
                     /locus_tag="PKL11_04225"
     CDS             complement(11428..11886)
                     /locus_tag="PKL11_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12841.1"
                     /translation="MPDPYVNKLLDTAHPCAAKSTVEGRINCVLDARAPQRGLELCPY
                     PSRAVLTHEIHELIITAEATAAPNATVNAIAYLGYFEVLQGGVLWVGDRVMINGQEVG
                     TLAGYDLTHFPNHLNIIVKASGPVQTGLELGYVPGTPLSFIFPGRPAEGA"
     gene            complement(11902..>12153)
                     /locus_tag="PKL11_04230"
     CDS             complement(11902..>12153)
                     /locus_tag="PKL11_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12842.1"
                     /translation="ALLKEARELGITPGVLTNLPLRLVTVNPFYPRYLQRDYRYEAAY
                     VDAERLLASVRGQVTTAPVADLVAHPPADLLKIILGEYH"
     assembly_gap    12154..12253
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12254..13073)
                     /locus_tag="PKL11_04235"
     CDS             complement(<12254..13073)
                     /locus_tag="PKL11_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12843.1"
                     /translation="MQTIGFAGTAKNTGKTTAALKVLGLARDAGLRLAVTSIGYDGEQ
                     RDHVTGLPKPRYALPEGALLATAEQTLPICGPLEVMETTPIQTILGRVVIARMKEPGP
                     AALAGPNTRADIETTLARLDALGAELCLLDGALNRMAPMMAADGLVLSTGAAFDEVID
                     ALAGHAGALAGLFALPRLPLPLGALITPSLLTPAAAENATLTLLHGAPGWVLWGAVSP
                     RLCASVLEQATPALKGRALLFEDPVKLAVSGDPRDWAALLKEARELGITPGVLTN"
     gene            complement(13137..14930)
                     /locus_tag="PKL11_04240"
     CDS             complement(13137..14930)
                     /locus_tag="PKL11_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12844.1"
                     /translation="MKTVLLAPLDPVHDNAIKLLKRKLTDAGYRAVALPPGSTEEEVV
                     ARALEEHPIAILVSRTLGYKVAETLGHLVDLAEAAGLRPQTRLGVGGMAITKELGAEL
                     GFDGCFVGSLDMTELISFLEGHAVAEAAQASQAAPRPRPDVTAGFSYAWRDATIAGLL
                     DQITDQILAWTVDKTSDAIARAELRAEMLAHPSAALRERYIALCDAEIQAFYRQGALP
                     AGVRRLEEAELAALPRATAGPRPAFESLRHGREKPLFFVQYGTGCPIMDITHIRLAED
                     WGVDGVLHFDPSWGAQREGLLEGLLTHEHDGTIVTLGNLKLIRQYMNPATLWNVRGHR
                     GLNTPETQVLAQAAGADLLKINIPYGSTGGGTDPARLTVDGVYSLRLAAKYGLPFDIP
                     GNDELSGVPPHKTFAGLLIMVMLGLKLGARPIPKPLLCYSPYMTVEGLMDADFVDMNF
                     AKLRVWREIVDAPVWPGEPVGFMTHSPERVQSAVTTAAHAALASSAGVAAVTIASSDE
                     AYSKGPISAPARVDSLRATRELLRFFGTAEIQPTPRADALQAELHTGITATLTAIARR
                     GDFVAAIYEGLLGSQEDGLYPGRTGKGTVRI"
     gene            complement(14927..15163)
                     /locus_tag="PKL11_04245"
     CDS             complement(14927..15163)
                     /locus_tag="PKL11_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12845.1"
                     /translation="MTQALSRVVDALDIETFLYCKDEDEAKTLAEALAAELGLPHGDV
                     VFVEHRGAGARVRIRSYIHHPGDHYRWLGAEDKE"
     gene            15263..16687
                     /locus_tag="PKL11_04250"
     CDS             15263..16687
                     /locus_tag="PKL11_04250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNZ12846.1"
                     /translation="MSLSGSKEHYLTLFEYAPISLWEQDFSGIRQQFDALRSQGVTDL
                     AAYLDAHPEFVMTCMRKMIVVAVNQKTVALFGAADQADLLANLPRVFRDGMRHHFRSE
                     LLALWNGELTWSGEGVNYTLSDQPVDILLHWRILPGFEQTWSQVLVTLEDITLRKQSE
                     QRLQAMFEASPISLWEEDYSGVKAFLDEVRQSGVDDLLEYFDLHPEAVAHCMSLIRVL
                     DVNQRTLELFGAESKEHLLSNLNRVFRDEMGTHFARELADLYNGRLSYEREGINYSLT
                     GEPINIHLDFRVMPGHEDDFAWVLVAIQDITARKKAEDYLRYLGTHDVMTGLYNRLYF
                     DETLLRLEKHRHDPISIILADLNGLKRVNDSLGHPAGDNLIRRAAEVLKASFDEDVTI
                     ARIGGDEFVVILPEVDADGAAEGLMRIRALIDLNNKYYREPELSISLGAATSEPGLPL
                     ARVFSQADDAMYRHKKDHHRLAGL"
     gene            16730..17497
                     /locus_tag="PKL11_04255"
     CDS             16730..17497
                     /locus_tag="PKL11_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNZ12847.1"
                     /translation="MTSYLIDMDGVLVHGQRPIPGADAFLARLQTRGIPFLILTNNSM
                     FTPGDLAHRLQSAGLKVRPEQIFTSAMATARFLTVQKPNGTAFAIGESGLTGALHDAG
                     YILTELDPDFVVLGETFTYNYDQIKKAVRLVMAGAHFIATNPDPIGPGEHGVVPACGA
                     MAALIETASGRKPFFIGKPNPFMMRAALHYLGVHSENTIMIGDRMDTDVVAGIQSGLG
                     TILVLSGVTAREQIADFPYQPGRVVDSVAEIETGEAA"
     gene            17494..18270
                     /locus_tag="PKL11_04260"
     CDS             17494..18270
                     /locus_tag="PKL11_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNZ12848.1"
                     /translation="MKLDAQTAGGGRITALDAVTQRLSLPPGDGRTYRLAQLDDYTRL
                     PRKAFRWQAPARLELRARVSAPDLPGTWGFGWWNDPFSAGLGLGGGQRRMPTLPNTAW
                     FFYASPPNHLAFRDDHPADGLLAAVFSAPRALGLMLAAGLPALPLLAWRVTARAMRRT
                     ASRFIKEDAVRVDRDVTAWHTYAIDWQPTGVHFSVDGQPCFETPIAPEGPLGLVVWID
                     NQYAAFPPDGRVRMGALPCATASALELTDLTIESTRSVVK"
BASE COUNT         3579 a         5580 c         5075 g         3837 t
ORIGIN      
        1 gccttctggt tgggctggcg ctgcagcagc attaatgtga tgagtcggct ggcgggtttg
       61 gtcataatct atccatatga tagcctatgt atatgtcatt ttacgacata attatagcct
      121 gatttgtaat tcgcccccag agaaaggaat tgtgactgtc atgaaagtcc cgccgcgcta
      181 taatgagcat attcccatga acctcactta tcccccggaa ctatttgacc tgaattttgc
      241 gtttgtctac cgggtgtctg aaattctgga gcagccctgg cccgacctgc tgctgaacta
      301 tacccacacc ttccgccgct ttcgcctggg agcggtactt gatccccaaa atccgatctg
      361 gcttcagttc cttgaggggg ttcgccgggc ggatgagcct gcccggtacg cttatgcatt
      421 ttacctgcag cgtgagcacg acacgggtcc gctgcagcgc cagaatgcct ttggctgctt
      481 ctcctatacc ctggaaaagg ccgattgtga cacgatcttg cgcattcatt ttgccaacca
      541 gagtccggct ggcacaggac ctttgagcca tgaaaaccag gcgctgcggc ggacagaact
      601 gacagagatg tttattgatg cccggcaggt tgtgaagcag ccctgcctgg tacggggtgc
      661 ttcctggttg tacaatttat ccggctaccg gcgtctattc ccggctgaat tcatccgcag
      721 tctggtggcc cgcccacccg actttccgat gctggccctg tgggggcagt tcctggatca
      781 caactggcag atacgtcccg acctggccgg agtttttctg gagcgaattg gacgggccca
      841 gtcaatgaca gatcttgagc aggcgtttcc ctattcaatt ctgattgcct gtgccgggat
      901 tgaagccttt tgggcatttt atgacatcgg aacagcctgc caaaatccgg ccctgaaatc
      961 ctgaaattca aataacccgg gactgttgag cagaccggcg ggacgcattt tggtcaggcg
     1021 gtcattcact tttgctgcac tggcatatcg accccattgg gtaaagcgcc ttgttcagat
     1081 tgaattataa tgagtccggc atcacaccat taaaaagcag gcggcctctg ccgtaaaata
     1141 aatgaaattc atgtttttag gcggcgcaga tgaagtcggc gccagcagca ccctgatcga
     1201 aatcgatggc aaacgcatcc tggtggactg cggcattcgt ccctccccca aggcgcggga
     1261 tggtctcgcg ggcgaccagc tgccgaacct cgaccagatt acccgtccag acgccatcct
     1321 ggtcacccac gcccatacgg atcacaccgg tgcccttgaa ctggtaacag atcgttatcc
     1381 agatgttcca gtatatgcca ctgctccgac gatcgccctt attcgtgttc tgcaccagga
     1441 tgcgcggcgg atcatgcagt cacggcttga cgaggaaggc gaactgcccc tgttcgatga
     1501 tgtatcggtg cagcggttga tggctgcatt ggttccggtg ccatttctgt ctcgtgtccc
     1561 acttggtgaa ggggtgtctg tcactttttt cccggcggga catatcgcgg gggctgccat
     1621 gctggggctg gaaagttcag aaggcaacct gctcatcacg ggtgattttt cgatttctcc
     1681 ccagcgaacg gtggatggag cccgcccgcc cgcttttcac cccgatgtgc tggtggttga
     1741 aagcacttat ggcgggcggt tgcatgccaa ccggcatgtg caggaacgaa aattgatcga
     1801 gactgtggct gccgttactg gggcaggtgg caaggtgctg attcctgctt ttgccctcgg
     1861 gcgggctcaa gaaatcctgt tgatcttgag tgaaggccag cgccgcggtg agctgccgtt
     1921 ggtgcctgtt tgggccgatg gaatggtccg caccatatgc catgcgtaca ctggttttgc
     1981 cgatgtgttg ccattgtccc tgcaggaacg cggggctaga ttttttgagg gacacatcca
     2041 gccggttcaa acggccgagc agcggaatgc cctgatctgg cagccggagc cggcagtgat
     2101 cgttgccagc agtgggatgc tggcgggggg accagcgctt ttgtatgcca aagcatttgc
     2161 cggcctcggt cagcatgcca ttttgttgac cggttaccag gatgaggaat cgccaggccg
     2221 ccgtctgcag gagctggctg agaagggaca tggtaccttg cgcctgggaa acgaaaaagt
     2281 ggacgtgcag tgtcttttgg ggacctattc cctttccgcc catgccgacg aaggccaggt
     2341 ggtcagcctg attgaaacac ttaaccctga acgggtggtt ctggttcacg gcgatgaccc
     2401 ggcacgcgcc agtctggaaa aagctttggc tgagcgcgaa cgacttgtcg acctgcccca
     2461 tgcaggccag accctggaac ttcaatttga gcggcgaagg ccggggcggg gtgaaacatc
     2521 cggaattgac ttccgcgacc ggcaactgac attgacgcgt gagcgtcaga taaagatggg
     2581 aaattttacc ggatgtgtgg ggaagtggct gctgctgaaa ggatcagttc ccaccccggt
     2641 gcggtgtctg gctttggaaa atgatcacct gtgggtggag atcacccctg gattggaaca
     2701 atctgtctac ccggaagaga tcctctcggt gctgggagat accccgccag caccggcaga
     2761 tgtgaccttt gcccaatctg cttcagcgtc gcctgtggtc atggagccga accaggccat
     2821 ggctgctgcc aaccagcgct ttccagctgc tgcgcgttta cgaaaagttg gttaccacct
     2881 gaccgaacag agcctggtgc tcacatttga ctttcccggc gtggccagtc ggcagtttgc
     2941 tgaaaccatt gcggatttgc aaaaagccac cggttgggag gtggtggtga ccccggaggc
     3001 caatcaaggg gccctcaatg ccttcctgcg ggaagtactg cccgaagact ggcagatgac
     3061 caaaagcccc gcaattcatc gggaacagca gcgcgtggtt gcggcggtac gcgcccccca
     3121 atccagccag gtggatgctg tggaatcaat ttgctcgcgt tatcttgccg aaacaggtta
     3181 tgtgcttgaa ttggtggtga gccaggccgg tgccccggcg gattccccgg ttgttttgcc
     3241 cgtggcctgg cgaggcgagc gtttggaaat caacgcggcc tacaccctga tcaaaagcag
     3301 cctggagggc agtacgctct accgtacgag tcttaagggt gaaacgatca tgctgtcatt
     3361 catctccccc caggttggcg agcggtaccg acagcagatc gaggatctgt cggtccagat
     3421 tggctggcca ctgacgatga acctgcagcc caaccagggg gctattctgg aaattgcccg
     3481 ggcattaatg aatgcggttc actggcaggt ggtcaaagga ccaggcgtgt atcccgagaa
     3541 ggccgaggtt atagtcagcc tgggaaacgc acctgctgaa acagaaattt ctgaattggc
     3601 ggcatccttt gaagatcaaa ccgggtaccg gcttgtggtc ttcgcggcca gaccagccct
     3661 tgcagaccag aaatcccccg cacttgatgg ccaggtgatt gaaatctctt tggcacagat
     3721 ccgcttgggg cattcgcatg cggcgatctc cctggatcca ctcaaacttg aaaaggcgat
     3781 tgaacgcgcc cgccggatgg gaattacccc gccgattcag gttcgccgca cgcgtacaga
     3841 ttatgtcctt gtggacggcc tgtaccgcct gcgggctgcc caggcgctca acctcgaaaa
     3901 agtccccgcc attgtggagt agtgtcagtt tcaaccggct tgtgcttgag ctggatcgct
     3961 ccaatccggt taaaaatcag gctctgggag tgacgctccc aaacaaccac ccggcacggt
     4021 tcagcgcatt aatgggcggg tccgggcggc tgaccgggca gattttaatg cccgggcgca
     4081 aagccacctg accgcccttt tgacttggag cagcccggtg tctttgtgtt tcctgatctc
     4141 tggcaaaacg gacgccgaaa aattaaagca gccgttcgcc gaccgtgaca ctgtccaccc
     4201 ggtaatgtcc cccccggctc tggcggttgt gccgtgctgc cttggcgata atcaacgcag
     4261 cttcaaccgc attccataag ccgatcaacc catcgctcaa cttggtcttg cgatagaagg
     4321 tctcaatttc attctgcagg tgacgcagct ctcgaatggc gcgcgacagc cgctcacccg
     4381 agcggatcag tcccacatag tgccacatga tattctgaat cgtctgcatg tcgccctgaa
     4441 tcagcgccgg gtctgaatcc tgcgtcagcc ccgattcatc ccagatggga atatccaccg
     4501 gctgtggcac aaatgcgcct tcagccagtt ccgctgcaat ctggtctgcc gcccgcgccc
     4561 caaacaccac cccctcaagc agcgaggcgc ttgccaggcg gttggcgcca tgcagcccat
     4621 tgcagcttac ctcgccaacc gcaaacagat tgcgcacatc agtccgcccc cacaggtcca
     4681 ccgccacccc gccgcagaag taatgcgccg ccggcaccac cgggatcggc tcgtgtgaca
     4741 tatcaacccc ggctttcagg caggtggcgt atatattcgg gaagcgcgcc tggatggctt
     4801 cgggcgccat gtgcgaggca atatccagca gcacatagct gtatccgtgc gcctccatct
     4861 ctgtatggat ggcccgcgcc accacatcgc gcggtgccag atcgcgccat tcgggagagt
     4921 accgctccat gaagggcacc ccctccggcg tcagcagcac tccgccttcg ccccgcacgg
     4981 cttcactgat cagaaagccc tcggctcccg gaaccgccag cgccgttgga tgaaactgca
     5041 catactccgc gttgatgatc cgcgccccgg cccgatgtgc cattgccagc ccgtctcccc
     5101 gggcgccggg cgggttggtg gtattgcggt aaatgcgccc cacgccgccg gtcgccagga
     5161 tggtcttggc tgccagggtg cgatgaatgg cccgcccgtt ctggtcaaac acgtaggccc
     5221 catagcaggt caccgggccg taagtgtcca gcggattgcg ggagtgatgc ggataagtga
     5281 tcaggtccac cgcagtggcg cgggtcagca gctcaatgtt ggggcggttc tgcaccgccg
     5341 ccatcaatcc ttgaatgatg gccttgcccg tgccatcgcc aacatgcaaa attcggcggc
     5401 aggaatgggc cgcttctttg gtataggcca ggctgccatc tgggctgtgg tcaaaatgga
     5461 ccccgccttc gtgaatcagc aggtcttcca gcagttctgg cccctcacct gccagaattg
     5521 ccgccgccgc cgggttggtg gcccccgccc ccgcgttgat aatatcggcc gccagcagtt
     5581 ctggagtgtc tgcttcgccg cggtggatga tgcccccctg ggcgtagttt gagttggatt
     5641 cgtgggggtc agtttcgcgg gtaatcagaa taatccgccg gttggggtca cgcgccagcc
     5701 gcagcgccgc gcaggccccg gcaatcccgc cgccaatcac aagcacatcg gttattttca
     5761 tgctttttgc ctagccaatc cgaatcatac ggtccacagc ctggaaagcc cggacgcgaa
     5821 tttcttccgg gacttcaatt acccagcggt tatgctgcag cgcatcccgc acattctcca
     5881 ttgtgatcat gttcatatgc gggcagcgca ccatgcacag ccgcagcaga tttttctccg
     5941 ggttggccgc ggcaatattg tcgcccatcg cacattctgt caaaatcagg taatgcgggg
     6001 cgttgctctg ctgcacatat ttgatcatcg cattggtcga gccggagtaa tcggctgccg
     6061 ccaccacttc gggcgggcat tcggggtgtg ccagcaccgc cacatcgggg aactgcgctc
     6121 gcaccgcctg gatatcggcc acagtgaatt tttcatgcac ttcacagcgg cccgtccagc
     6181 cgatcatttg cacatccagc agggtttctg cctctggcgt ggttcccagc cggctgggga
     6241 agatgatatg cttgccggtt tcccgggcta cgttgcccgc caggtactga tccggcagga
     6301 agattaccgt gtcgctgttc agcgattcca ccactgccac cgcattgccc gatgtacagc
     6361 aaatatcgct ctcggctttc acatcggcat acgtgttcac atacgtcacc accggtacgc
     6421 cgggatattt gcgtttcagg gcgcgcacat cctctgccgt gatggattca gccagggagc
     6481 agcccgcccg ctcggccggc agcagcaccg tcttggtcgg gcttagaatc ttggccgttt
     6541 cagccataaa tttgaccccg caaaagacga tgacatcctt atcggtctgg gcggctttgc
     6601 ggctcaggtc caggctgtca ccgcgaaaat ccgggatcgt gtagaacaac accggttcca
     6661 tgtagttatg tcccagaata acggcgttgc gctctttttt gagtgcgttg atctccacgg
     6721 caatttcggc tttgtagcga agttcaaaat ccggcacaaa ctgtccaagc cgggttttca
     6781 tttcttcata aagtacctgc gcttcactat tcattatcct ctccttcggc ctgcttgctt
     6841 aacggataaa atccaggctg acatcaaaaa ctttggctga gtgggtcagt gccccacttg
     6901 aaatatagtc cacgccggtt tcggcaatgg cccgcactgt ttccagggtc acattcccgc
     6961 tggcttccag tttggctcgc ccgacgttca aaaccacagc ctggcgcatc gtttccagtg
     7021 acatattgtc cagcagaatc cgctccacgc ccagttccag cgcctcttgc acatgcgcca
     7081 gtgtgcgcgc ctcaacctca atttccagcc cggtatgggc cgcgcgggct cgctccactg
     7141 ccgcggtgat gccgcccgca aagtcaatat ggttatcctt gatcaaaatc atatcataca
     7201 gcccaatgcg gtgattctgc ccgccgccgc gggccacagc cagcttgtcc agcatccgca
     7261 gacccggcac ggttttgcgc gtatccagga tgactgcccg ggttccagcc acggcatcta
     7321 caaactgacg cgtcagggtg gcaattcccg aaacccgccc caaaaaattg agcgccgttc
     7381 gctcgcccgt caacaggctg accgccgggc cagcaagccg gctgaccacc tgccggtttt
     7441 ccacccgggc gccttcttca accagggcag taaattccac ccgtggatcc aacgcggcaa
     7501 ataccgcctg ggccacatcc agccctgcaa tgaccccact ctgcttggca ataatctggc
     7561 cggtcatcac cgcatccgcc gggatgatgc tgttcgaggt ggcatctccc gcaccaatat
     7621 cttccctcag ggcgcgttga atggcttcga gaatatcagg atggatgctc atgggattaa
     7681 ctccttgaac cgctaaaatc aatcgaggtt gatcagccca ctgggcgcaa cctgtactcg
     7741 aattctagcg ggtttgacag gccgggtcaa ggccggatta tccccgcctc atttcaaaag
     7801 gcctgctcga tcatctgcgc ggtcagccgc acactgctct ccaaccgaat ctgttccccc
     7861 agcatttgag cctgctgccg catctgggta tcgcggagcg tctcaatcaa cgccagcgcc
     7921 acccgttctg aacttaattc agggtagggg atggctttgg gcccgcactt caattgataa
     7981 atccgctgcg cccaaaaatc ctggtccacg gtttgcggaa tgctcaccgc cggaatcccc
     8041 gcccgcagcc ctgccccggt gatggtgaac ccggcataat gcaccagcgc acagcacttg
     8101 ggcagcagcc aggagggcgg aatccacccg ctgaccatca cggtgggtgg caaatcaatt
     8161 ttgcgggcag cttcctggct gccgacgatg atggcgcgca cacccgcaaa ccgggtggcc
     8221 tccacaaaca tcttcagcag gtcaatcgtt tctttgccgc tcaagctgcc gcccagccag
     8281 atcgccaccg gcggcccgcc agcatccaga aaggcgcgaa tttcaaccgg cgagctccag
     8341 cccggcggtt cgcccagcag ccagtacccc accatgcggt gctgtggcgc ccagcgtggg
     8401 tcaggcgtga ccaccagcgg gctgaccggc accaggttga gccggccaga catcatattt
     8461 tccaggctgc ccttgatggg aggcaggttg agctgcttgc gaatgtgatt gtagggtcct
     8521 accatcatgc tgttcatcac tgcgccaatc accgcactgg tggcccggtt gatgcccgtc
     8581 tgatggctct cgaccaccgg gataatctgc ggctgaatgg ttacactgat aaacgggatg
     8641 cctgccttgt ccgattcggc gcgtccggca aagctgtgcg tcacaatcat caggtcggca
     8701 cccgggcaca gcttgaggat atctttagag cagcgttcca caatcgacgc ggtgaattcc
     8761 atcaggctga taaagcccaa caccttgttt ttggcccggg tgcgaatctt ttcagcttcg
     8821 tttcccaggt ggatgttggg gccaatgctg gcaaatgtca gcccgtggct tttaatccat
     8881 tgccccatcc ccgggtggct ggcgatggtt accgtgtggc ctgcgcgctg caaccccaga
     8941 cccagggcca gatagggctg aacgtcgctg cgtgttccaa tggccggaat aaggattttt
     9001 cgcatctggg ccagggcccc caatcacgga taaagaatat cgcctgggtc ttattataca
     9061 acgattttaa gccgggccca tctaaattgt ccggctgaaa gttacggcag tctgaatgct
     9121 acagacccat caggtgcgag accagaatac ggatgccgat tccaattaag atcagcccgc
     9181 ccagcacttc catccgtttt ccaaaacgcg cccccagccg ctgccccagc cgcacaccca
     9241 ccagcgacag cccaaacgtg accagggcga tcacgctgca ggcaatggca atcttgacct
     9301 cgatcagcgc catgctcaac cccaccgcca gcgcgtcgat gctggtggcc acacagagga
     9361 ttaccagtgt gcccccgcgg gatggatcct tgccggtctc aacttcttct tcagtatgat
     9421 tcttaagccc ttcaaccaac atacgcccac ccacaaaagc cagcagggca aaggcaatcc
     9481 agtgatctac gctggagatg aaccgttcca cggtgctgcc ggccagccag cccagcagcg
     9541 tcatgccgcc ctgaaagaac ccaaaatgaa aagccagccg gaaggctgcc cgcatattgc
     9601 gcccattggg agaagtgcca atgcccagcg aaactgcaaa ggcatccatg gcaaccccaa
     9661 ctgcaattaa cagggtggta atcagatcca tcttcactcc agttcaatgt ctgacaaaca
     9721 gcccgattat actggaaaac cccggctgct gcatgtaact tttatcctgt cttgacgtct
     9781 aatcagatag ttctagtcta tataggagat aacatatgaa atgcatcatg ccaaatgaat
     9841 caatgaccga tcgcatcatt cgggttgtgc tgggcattgc cctgctggcc ctgggctgga
     9901 cgggcactgt gagcggcgtg ctgggagaaa tctttaagta cctcggtttt gtccccctgc
     9961 tcaccggcct gatcggcttc tgcccgctgt atgccctctt caagttccgc accaacaagg
    10021 gctgattgcc tctaacccat tccgtcttgc tgcgcggcac ttgaccattg aacagcccaa
    10081 aggtacaatt gggccgttaa ccaatgcggg agtcgccaag tggtaaggca tcagccttcc
    10141 aagctgacat tcgcgggtcc gaatcccgtc tcccgctcnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnta aggcatcagc cttccaagct gacattcgcg ggtccgaatc
    10321 ccgtctcccg ctcctcgacc aaaatgcccc gttcagacgg ggcatttgtt tttcctcatt
    10381 gccacttgca ggccctatcg aagggtggta tccgaatccc gtctcctgat tttaaaaaag
    10441 gagtccctgc cttaacccgg caaggactcc ttctaaaccc aaaacccaaa acctataccc
    10501 taaaacctat atctctaaac cagcctcttc accaaccccg tcaaatcttc cggcaggtac
    10561 atataaggcg ggatttccag cagagtatgc gccccggccg aaagccgggt gcaggctcgg
    10621 gcgcagccca ccatcacctg ggctgttgaa gccgggttgt ggaaggttgt ctcaaaatgc
    10681 agcagttgat tatccacccc gctggcgccg cccttgcgcc gcacatcaat ccggtgcccc
    10741 atatccacca gcggcgtcac atcatccacc tgaaaaacgc gcacatcatc atggcagaag
    10801 taagagtcct gcaaaattgc cgccttgacg ctttcaaaat cggcgccggg ctccaactcc
    10861 agatacagat cacgcttatg gcagcccggg tcgccaggcc gagtgatgga gagcgcatct
    10921 ttcacgcccg cgtaagattt agccgccacc gtgtgcccca tgctcatccc cggtccatag
    10981 gtcacatacg tcagcccggt cggcgcccag atctcaaaca gtgcccggat gagcgtactc
    11041 acacccggat caaccccggc gcccattacc gctgcagcct ggttcgcacg ggcaatcggg
    11101 tccagcgtgg ccttgagctg caggaacggc ccgccgtgca tgtcaaagca gtccaccgta
    11161 tgaataccct gcgccagcag cgtgggcgcc actttttcca ccgtccgggt cggcagcgcc
    11221 agaataacca ccgtcggctt ttctggcagt gcttccagcg aggtcgccag ggtcacgccg
    11281 ggcagttcgt gccgcacggc ttcaatccgg gcgggcagtt caaccgcgcc gatcaaatgc
    11341 atatcagcag ccagttgagc cgcgcgcgcg gcgtaaattc caatatggcc gcagcccatc
    11401 acggccactt taatggggtc actcatctta cgctccttcc gcgggtcgcc ccgggaaaat
    11461 gaaactcagc ggggtgccgg gcacatagcc cagttccagt cctgtttgca ctggcccgct
    11521 ggctttgaca ataatattga gatggttggg gaaatgcgtc aggtcgtagc ctgccagggt
    11581 gcccacttcc tgcccgttga tcatcacccg gtcgcccacc cacagcacgc cgccctgcag
    11641 cacctcaaaa taacccaggt acgcaatggc gttcacggtg gcgttggggg ctgcggtggc
    11701 ttcagccgtg atgatcagct cgtggatttc gtgcgtcagc acagcccgtg aggggtaggg
    11761 gcacagttcc aggccgcgct gcggggcgcg cgcatccagc acacagttga tgcgtccttc
    11821 caccgtgctt ttggcggcgc aggggtgcgc tgtatccagt aatttattca cgtagggatc
    11881 aggcataatg gctccttgtt tctaatggta ctcgcccaaa atgattttta acagatccgc
    11941 aggtgggtgc gctaccaggt ctgccacagg tgcggtcgta acttgccctc gcacagatgc
    12001 cagcagtctt tcggcatcca catacgcggc ttcgtagcgg taatcacgct gcaaatagcg
    12061 cgggtagaac gggttgaccg tcaccaggcg cagcggcaga ttcgtcagta cccccggtgt
    12121 gatgcccagc tcgcgggctt ctttcagcag ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnngattcgt cagtaccccc ggtgtgatgc ccagctcgcg ggcttctttc
    12301 agcagggcgg cccagtcacg cgggtcgccg ctgacggcta acttaaccgg gtcttcaaag
    12361 agcagcgccc tgcccttcag cgccggggtg gcctgctcga ggacggatgc gcatagccgt
    12421 ggtgatactg caccccacag tacccatccc ggtgcgccgt gcagcagggt taatgtggca
    12481 ttttcagccg ccgcaggcgt cagcagggat ggtgtgatga gcgctcccag tggcagcggc
    12541 agcctgggca gcgcaaacag cccggccagt gcaccggcat gtccagccag cgcatcaata
    12601 acctcatcaa atgccgcccc cgtggacagc accagcccat ccgccgccat catcggcgcc
    12661 atgcggttga gcgctccatc cagcaggcac agttcagcgc ccagcgcgtc caaccgtgcc
    12721 agggtggtct cgatatcggc gcgggtattg gggccggcca gcgcagcggg gccgggttcc
    12781 ttcattcggg caatcaccac ccgccccaga atggtctgga tgggggtggt ttccatcact
    12841 tccagcggac cacagatggg cagggtttgc tcggccgtag ccagcagggc gccttctggc
    12901 agggcatagc gcggtttggg cagcccggtc acatggtcgc gctgctcgcc gtcataacca
    12961 atactggtca cggccaaccg cagcccggca tcccgcgcca atcccagcac cttcagcgcc
    13021 gcggtggtct tacccgtatt cttggccgtt cccgcaaatc caattgtttg catgagtatt
    13081 tacgacatct gttgtgttct gtaaatcagc aatctaaaat ccaaaattca aaaatcctaa
    13141 atcctcaccg ttcccttacc ggttcgcccg gggtacaacc cgtcttcctg actgcccagc
    13201 agaccctcat aaatggctgc cacaaaatcc ccccgacggg caatggctgt cagcgtggct
    13261 gtgatgcccg tatgcagttc tgcctgcagg gcatctgcgc gcggcgtcgg ctggatctct
    13321 gccgttccga agaaacgcag cagctcgcgc gtggcccgca gcgaatcgac ccgcgccgga
    13381 gccgaaatag gccccttaga ataggcctca tcggatgagg caatggtcac agcggctacg
    13441 ccggcgctgc tggccagcgc agcatgggcc gcggtggtaa ctgcagactg cacccgttct
    13501 ggtgagtgcg tcataaagcc taccggctcg ccgggccaga ccggtgcatc gacaatctcg
    13561 cgccagacgc gcagcttggc aaagttcatg tccacaaagt cggcgtccat cagcccttcc
    13621 acggtcatat agggtgagta acacagcaag ggcttgggga tcgggcgtgc tcccagcttg
    13681 agccccagca tcaccatgat cagcagcccg gcaaaggtct tatgcggcgg cactccgctc
    13741 agttcatcgt tgcccggaat atcaaacggc agtccatact ttgccgccaa ccgcagcgaa
    13801 taaaccccgt ccaccgtcag gcgggccggg tcggtgccgc cgcccgtgct gccgtaagga
    13861 atattgattt ttagcaaatc ggctccggca gcctgcgcca gcacctgcgt ttcgggcgtg
    13921 ttcagcccgc ggtgcccgcg cacattccac agcgtagccg gattcatata ctgccggatg
    13981 agtttcagat tacccagtgt cacaatggtg ccgtcgtgtt catgggtcag cagaccttcc
    14041 agcaggcctt ctcgctgtgc cccccacgag gggtcaaagt gcagcacgcc gtccacgccc
    14101 caatcttctg ccagccggat atgggtgata tccatgatcg ggcagccggt gccgtactgc
    14161 acgaagaaca gcggtttctc gcgcccgtga cgcagagatt caaatgccgg gcgcggtccg
    14221 gcggtggcgc gtggcagggc tgccagctcg gcctcttcca accggcgcac accggcaggc
    14281 agtgctccct ggcggtaaaa tgcctgaatt tcggcgtcac acagggcaat atagcgctca
    14341 cgcagcgccg cactggggtg tgccagcatc tcggcgcgca gttctgcccg cgctatagca
    14401 tcgctggtct tatcaactgt ccaggccaga atttgatctg taatctggtc gagcagcccg
    14461 gcgatggtgg catcgcgcca ggcatagctg aatccggcgg tcacatcggg cctcgggcgg
    14521 ggggccgcct gagatgcctg ggcggcttcg gccacggcat ggccctccaa aaagctgatc
    14581 agttccgtca tatccagact gcccacaaaa cagccatcga aacccagttc agcccccagc
    14641 tctttggtaa ttgccatgcc gcccacgccc agtcgggttt gcggccgcag tccggcagct
    14701 tcggccaggt ccaccaggtg gcccagcgtc tcagctacct tataccccag cgtccggctg
    14761 accaggatgg caatcgggtg ttcttccagc gcccgcgcca ccacttcctc ttcggttgag
    14821 cccggcggca gtgccaccgc ccggtagccg gcatcggtca gcttgcgttt gagcagtttg
    14881 atggcgttat cgtgaaccgg gtcaagcgga gccagcagca cagtcttcac tctttatcct
    14941 ccgcacccag ccagcggtaa tggtcgccgg gatggtggat gtagctgcgg atacgcaccc
    15001 gcgcccccgc gccgcgatgc tccacaaata ccacatcgcc gtgcggcagg cccagctcgg
    15061 cggccagcgc ctcggcgagt gttttagctt cgtcttcatc tttacagtaa aggaatgttt
    15121 caatatccag cgcgtcaacc acgcgcgaca gggcctgggt catggcggtc tccttgatgg
    15181 taggttgtgt gttattatac cgcgcccgca tcaacggtaa ctttgctata atgagattgt
    15241 tgctgtgcaa aaggaggtct ttatgagcct atccggcagc aaggagcatt acctgacctt
    15301 gtttgaatac gcccccatct ccctctggga acaggatttc agcggcattc gccagcagtt
    15361 tgacgccctg cgctcgcagg gtgtgacgga tctcgccgcc tatctggatg cccaccccga
    15421 gtttgtgatg acctgcatgc gcaagatgat cgttgtggcg gtcaatcaaa aaaccgtggc
    15481 gctttttggc gccgccgatc aggccgatct gctggcaaac ttgccccgcg tcttccgcga
    15541 tggcatgcgt caccacttcc gcagcgaact gctggccctc tggaacggtg aactcacctg
    15601 gtctggcgag ggcgtcaatt acaccttatc cgaccagccg gtggatattc tactgcactg
    15661 gcgcatcctg ccggggtttg aacaaacctg gtcgcaggtg ctggtgacgt tggaagatat
    15721 caccctgcgc aaacagtctg agcagcgttt gcaggccatg tttgaagcct cacccatctc
    15781 cctctgggag gaggattaca gcggcgtcaa ggcttttctg gatgaggtgc gccagtcggg
    15841 agtggatgat ttgctggagt attttgacct gcatcccgaa gccgtggccc actgcatgag
    15901 tctgatccgg gtgctggatg tcaaccagcg gactttggaa ttgtttggcg ctgagtctaa
    15961 agaacacctg ctctccaacc tgaaccgggt ctttcgagat gagatgggca cgcattttgc
    16021 ccgtgaactg gccgatctct acaacggtcg cctgtcttat gagcgtgagg gcatcaacta
    16081 ttcgctgacc ggtgaaccca ttaacatcca tctggatttc cgggtgatgc ccggtcatga
    16141 ggatgatttt gcctgggtgt tggtcgctat tcaagatatt actgcccgca agaaggccga
    16201 agattacctg cgctacctgg gcactcacga tgtgatgacc ggtttgtata accggctcta
    16261 ttttgatgaa accctgctcc ggctggagaa acaccgccac gaccccatca gcattatcct
    16321 tgcagacctg aacggcctca agcgtgttaa cgattcgctg ggacaccccg caggtgataa
    16381 cctgatccgg cgggcggcgg aggttctaaa agccagcttt gatgaggatg tgacgattgc
    16441 ccggattggc ggggatgaat ttgtggtcat cttgcccgag gtggatgccg atggcgcagc
    16501 cgagggcctg atgcggattc gcgccctgat tgatctgaac aataaatatt accgtgaacc
    16561 tgaattgagc atctcgctgg gcgctgccac cagtgagccc ggtttgccgc tggcacgcgt
    16621 gttcagccag gccgatgatg cgatgtaccg gcacaaaaaa gatcaccacc gcctggcggg
    16681 tctgtaatcc ccttggctgc attgtaattc acattccagg agcttctcga tgaccagtta
    16741 tttgattgac atggacggtg tgctggtgca tgggcaaaga cccatccccg gcgccgatgc
    16801 gtttttggcc cgtcttcaga ccaggggcat cccgttcttg attttgacca acaattcaat
    16861 gttcaccccc ggcgatctgg cccaccggct tcaatcggct ggcttgaagg tgcgccccga
    16921 gcagattttt acctctgcaa tggccacggc tcggtttctg acggtgcaaa aacccaacgg
    16981 caccgccttt gccatcggcg aaagcggcct cactggcgcc ctgcatgatg ccggctacat
    17041 attgacggaa ctggacccgg atttcgtcgt ccttggcgag acctttacct acaattacga
    17101 ccaaatcaaa aaagcggttc gcctggtgat ggcgggtgcc catttcattg ccaccaaccc
    17161 cgaccctatc ggcccaggtg agcatggcgt ggtaccggcc tgcggcgcca tggccgccct
    17221 gatcgaaact gccagcgggc gcaagccatt ctttatcggc aagcccaacc cctttatgat
    17281 gcgcgccgcg ctgcattacc tgggcgtgca ctccgaaaac accatcatga tcggcgaccg
    17341 tatggatacg gatgtggtcg ccgggattca gagcggcctg ggcaccattt tggtgctttc
    17401 tggcgtcaca gcccgtgagc agattgccga ttttccctac cagccgggcc gggtggtgga
    17461 ctctgtggct gaaatcgaaa ccggagaggc cgcatgaagc tggatgccca gacggccggt
    17521 ggagggcgga ttaccgccct cgacgctgta acccagcggc tcagtttgcc gccgggggat
    17581 gggcgcacct accgcctggc gcagctggat gactacaccc ggctgccgcg taaagctttt
    17641 cgctggcagg cccctgcccg gttggagctg cgggcgcgcg tcagcgcacc agacctgccc
    17701 ggcacctggg gcttcggctg gtggaacgat cctttcagtg cgggtctggg gttaggtggt
    17761 ggtcagcgcc gcatgcccac tttgcccaat acggcctggt ttttctatgc ctcgccgccc
    17821 aaccacctgg cctttcggga tgatcacccc gcagacggac tgctggcggc ggtcttttct
    17881 gccccccgcg ccctgggact gatgttggct gccggattgc cggcgctgcc tctgctggcc
    17941 tggcgggtca cggcgcgggc catgcgccgc accgccagcc ggtttatcaa agaagatgct
    18001 gtccgggtag atcgcgacgt aaccgcctgg cacacctatg ccattgactg gcagcccacc
    18061 ggagtgcact tctctgtaga tgggcagccc tgctttgaaa cccccattgc ccccgaaggg
    18121 cccctggggc tcgtcgtttg gattgacaac cagtatgctg ccttcccccc agacgggcgg
    18181 gtgcgcatgg gcgcactgcc ctgcgccacc gcatctgccc tggaactgac tgacctgaca
    18241 atcgaatcaa cccggtctgt ggtaaaataa c
//
LOCUS       DAOPVS010000028        17650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3251_length_17650_cov_59.104291, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000028 DAOPVS010000000
VERSION     DAOPVS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17650
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3251_length_17650_cov_59.104291"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..256
                     /gene="rpmG"
                     /locus_tag="PKL11_04265"
     CDS             107..256
                     /gene="rpmG"
                     /locus_tag="PKL11_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNZ12849.1"
                     /translation="MRPVITFACNECKERNYTSEKNRRNDPGRMEIKKYCPRCHKHTT
                     HRETK"
     gene            320..392
                     /locus_tag="PKL11_04270"
     tRNA            320..392
                     /locus_tag="PKL11_04270"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:353..355,aa:Trp,seq:cca)
     gene            458..748
                     /gene="secE"
                     /locus_tag="PKL11_04275"
     CDS             458..748
                     /gene="secE"
                     /locus_tag="PKL11_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNZ12850.1"
                     /translation="MVKTKDTPSEKPVQPVAAKSKPVEKSAAKSDKPSKPNRIKQWWH
                     ETMGELRKVSWPTRQQAQRLTVIVLVTMLVMALFLGGLDFIFSELITLLVKI"
     gene            774..1439
                     /gene="nusG"
                     /locus_tag="PKL11_04280"
     CDS             774..1439
                     /gene="nusG"
                     /locus_tag="PKL11_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNZ12851.1"
                     /translation="MDAHDDFMPEEEGIEQPKERKPRRSTGRARTSEPVQNPASEETV
                     TESNERAWYVIHCYSGYENKVRHNLEQRIETMGMKDQIFDVVIPTQEEIEVKDGKRRT
                     VERHVFPGYVLVNMLLSEESWYVVRNTPGVTGFVGMGNQPTPLRPEEVQQILRRMEAE
                     APHIKVTFRVGERVRIVDGPFNDFRGTVSEIDMERTKVRVMVSFFGRETPVELDFLQV
                     EKA"
     gene            1513..1938
                     /gene="rplK"
                     /locus_tag="PKL11_04285"
     CDS             1513..1938
                     /gene="rplK"
                     /locus_tag="PKL11_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNZ12852.1"
                     /translation="MAKKLKAVVRLQLVAGKANPAPPVGPALAGYGLNIMAFCKEYNA
                     RTQNRTGDVIPAEISVYSDGSFVFILKSSPASVLLKKAAGVDKGSATPNRTKVGKVTR
                     AQVREIAEIKMKDTNAVDIEDAMRQVEGTARNMGITVVD"
     gene            1995..2708
                     /gene="rplA"
                     /locus_tag="PKL11_04290"
     CDS             1995..2708
                     /gene="rplA"
                     /locus_tag="PKL11_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNZ12853.1"
                     /translation="MAKHGKKYEAAKAKVDSKQNYEPRAAVALAKETAYTSFDATVEV
                     HMRMGLDPRQADQQVRDVVVLPHGLGKTVRVLVFTQGEGVLRAQEGGADHIADSDEWV
                     AKIQGGWTDFDVAIATPDMMGKVGKLGRVLGPRGLMPNPKAGTVVPAEDLPRVIEEAK
                     AGRVEFRLDKTANIHVPIGKASFDETKLFENFAALMEAIKKARPAAAKGIYIRRITLA
                     ATMGPGIHVDPNLAQNISL"
     gene            2922..3452
                     /gene="rplJ"
                     /locus_tag="PKL11_04295"
     CDS             2922..3452
                     /gene="rplJ"
                     /locus_tag="PKL11_04295"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNZ12854.1"
                     /translation="MAFTKQQKDKMVALYREWLRNAQGVVLVTFNKMSMKEIDTLRTK
                     IRDAGGEAHVVKNTLFYNALSAEGIHVAEKPEGTTLAVFSSDPASIAKALVEALGKSE
                     IFQIKGGFLGTELLNAAQVKSLAALPPLPVMRARLLGVLVAPASQLVRTLAEPARSMA
                     AVIKAHSEKEAVPAAG"
     gene            3570..3950
                     /gene="rplL"
                     /locus_tag="PKL11_04300"
     CDS             3570..3950
                     /gene="rplL"
                     /locus_tag="PKL11_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNZ12855.1"
                     /translation="MADLTKLVEELSKLSVLEAADLVKMLEEKWGVSAAAPVAAVAVA
                     AGPAAAAEPVEEKTEFDVVLKDAGPKKIDTIKVIRQLTNLGLVEAKTMAETAGAKVLT
                     AVSKEAATDAKGKLEAAGAVVEIA"
     gene            complement(4023..4754)
                     /gene="lexA"
                     /locus_tag="PKL11_04305"
     CDS             complement(4023..4754)
                     /gene="lexA"
                     /locus_tag="PKL11_04305"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNZ12856.1"
                     /translation="MARRSQKLSERQTRIMQFLTDFQESHGYSPSIREIGDHIDVKST
                     SLVDYYLRQLEELSYIRRDGRVSRSICVLQPVHGTVGGKVVQTVREAGSALRDLVNVP
                     LLGRIVASEPIPMPTQSGGAPGYFDPESSVEVARSMLPGRERIDQLFALEVQGDSMID
                     AMVNDGDIVILRPAQEANNGEMVAVWLDDRDETTLKYFYREPDRIRLQPANRTMQPII
                     VNDPAHLRIMGKVVMVIRQVQSVAA"
     gene            complement(4844..6361)
                     /gene="lysS"
                     /locus_tag="PKL11_04310"
     CDS             complement(4844..6361)
                     /gene="lysS"
                     /locus_tag="PKL11_04310"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNZ12857.1"
                     /translation="MERTELEKIRLEKLQRLLDQNILPYPTRAHVTHTAAQIIAAYEA
                     AEAAGDATPVAATLAGRLRALRPMGKVAFAHIEDGSGRIQLFFRINEIGPEAMDFLQQ
                     AFDLGDFIEAEGTMFRTRTGEATLQVQSFRMLAKAITPLPAAKDELVDGQVVRHAGLT
                     DPETRYRQRYADLAVNPEVREIFRARANTVRALRQFLDERDFLEVETPILQPIYGGAA
                     ARPFVTHHNQLHQQLYLRISFELYLKRLLVGGLDRVYEIGRDFRNEGVSYKHNPEFTQ
                     LEFYMAYADYLKIMEMTEQMIAFVCDQVLGRRTVNFRGNQVDLTPPWRRVDLRQGLIE
                     TTGIDIAQHPTAESLGAAMQAHNVPFKPGSPRGKLIDSLLGDFLEPTLIQPTFLYNYP
                     RDISPLAKSIPGDPTTVERFEGFVGGMELCNAFTELNDPLDQEARFQEMGRAYDDNDE
                     QRHPMDEDYLRAMSMGMPPNGGFGMGVDRLVMLLTDRSTIREVILFPHLRERDED"
     gene            complement(6431..6898)
                     /gene="greA"
                     /locus_tag="PKL11_04315"
     CDS             complement(6431..6898)
                     /gene="greA"
                     /locus_tag="PKL11_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNZ12858.1"
                     /translation="MHNTFLTREGYEKLQEELEFLRTTKRNEIADRLHEAMEGGELIE
                     NAEYEAAKNEQAFVEGRIKELEILLATAHVIDEAPAMGDTIQVGSTAIIREEGTTSDE
                     EYTIVGAAEADPARGRISNESPLGRALLNHKAGDRVQVDAPAGQFVVQIVKVQ"
     gene            complement(7130..7681)
                     /locus_tag="PKL11_04320"
     CDS             complement(7130..7681)
                     /locus_tag="PKL11_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ12859.1"
                     /translation="MQTEIIFSGFGGQGVLFAGQLLSYAAMDNDLEVTWIPSYGPEMR
                     GGTANCTVVIADEEIGSPLVRHPNAAVAMNLPSLDKYESVIKPGGVLVVNSSMINRPV
                     KRTDIVVVMVPANEIAERLGDKRMTNMVLMGALLANLKVLPLEALEKALQEHLPARHH
                     RFLPLNYQALREGAQIPAQPAKA"
     gene            complement(7694..8473)
                     /locus_tag="PKL11_04325"
     CDS             complement(7694..8473)
                     /locus_tag="PKL11_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ12860.1"
                     /translation="MVTQTTPEQTVYEHPDALVDCSTHYCPGCTHGVAHRLIAEVIDE
                     MGIRDRMIGVASVGCSVFAYNYFDFDFVQAPHGRAPAMATGVKRAMPDRVVMTYQGDG
                     DLASIGMGEIVHAAARGENISVFFLNNSNYGMTGGQMAPTTLPGQKTSTSPNGRDVEL
                     QGYPIRASELLSTLDGASYVVRRSLHDPKNIRLAKKAIRMAFETQLRGLGFSMVEMLS
                     TCPTNWGMQPQDAMKWLEANMVPYYPLGDYKIHPAVAEIKI"
     gene            complement(8482..9603)
                     /gene="vorB"
                     /locus_tag="PKL11_04330"
     CDS             complement(8482..9603)
                     /gene="vorB"
                     /locus_tag="PKL11_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNZ12861.1"
                     /translation="MARELMKGNEAVAEAAVRAGLGAYFGYPITPQTELLEYLSRRMP
                     ELGRAFVQAESELGAINMVYGAACTGTRVMSSSSSPGFSLMLEGVSYIAGTELPVVLV
                     DVMRGGPGLGNIAPAQADYNQVVHGGGHGDYHSIVLAPATVQEAVDLTVLAFDLAEKY
                     RSVTVVLLDGSLGQMMEPCELPPMAPLRGVSTAEWATTGTMGQRGRRILSSIYLNPPE
                     EEVANIRMLNRWQEVEANEVRYKTYFTEDAKYLVVGFGSAGRVAVSAVRAARAEGIPV
                     GLLRPITLAPYPTKVLDELVDKVDGLLVTEMNGGQMLEDVRSIVRGRKPIEFYGRLGG
                     IVPFPDEILGEIRRMAAGPLPTDGHPRDRWLKRMATVIE"
     gene            complement(9605..9847)
                     /locus_tag="PKL11_04335"
     CDS             complement(9605..9847)
                     /locus_tag="PKL11_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNZ12862.1"
                     /translation="MPPKGRIEVKELTCKGCNLCVGACPQHVIALSEGINPKGYHPAT
                     LVAEGCTGCGICALMCPEAAITVYRETVNRPIKAEE"
     gene            complement(10020..10523)
                     /locus_tag="PKL11_04340"
     CDS             complement(10020..10523)
                     /locus_tag="PKL11_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12863.1"
                     /translation="MTTETERPLLALPPALSRLRDRFLAGWRNPRRRPLTLALVLIGA
                     LLLIVSCGLLLAGSFGTLPTAVDSPFEQILVGRWQLTADSPRPSGYNVELRFEEGGRL
                     WLVDRATGEGLSATYVFVAEKRVSIFISIGLPSYDFTFDYDGSVLSVETPPDLGFGTL
                     YFTRVSE"
     gene            complement(10520..>12049)
                     /locus_tag="PKL11_04345"
     CDS             complement(10520..>12049)
                     /locus_tag="PKL11_04345"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNZ12864.1"
                     /translation="GPVRLRVALANDYVVPLINLADQIGVLGIWRTAESLGLSGLSTA
                     APDADLLTSGGSLTVLQAAQGYSSFATLGLLNGRRAAVDEPLQPVLILLVQDSSGRAL
                     LDQQVSESQPVLSQPLAYLITHVLSDESARWPSLGHPNLLEIGRPAGAHLGQIADATQ
                     AWTVGYTPQRVAVVWAGSDIALNSDLSASLWHALMQYTHGGRPVENWTMPSGLQTVDV
                     CDPSGMLPTVNCPNVVSELFMAGSEPVMLDTLYQSLQVNRETGRLATVFTPPEQVEAR
                     VYLMAPPEAQAWARWMGLPLPPIEYDAIQAPPPLPNVQVTSPGLYAYVRGVVPIIGTA
                     AGTDFASYTVQVGQGINPRSWMQVGQASESMVVGGELAAWDTTDLEGLYAIRLVVVRD
                     DSQVDSAILQVTVDNTAPLVESPYPLADQTITPPTGQIVTLQAAVQEAVGVNRVEWWV
                     DGTLAGLRQGGEGLTSGDAMAFSLPWSAAPGRHTLVIKAYDLAGNLGESESISFTVSR
                     E"
     assembly_gap    12050..12149
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12150..13129)
                     /locus_tag="PKL11_04350"
     CDS             complement(<12150..13129)
                     /locus_tag="PKL11_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ12865.1"
                     /translation="MQRRHQFTPRLGRTARALGAVLGVLLAAVVVWAGFAWARLTADL
                     PSIQILPTLLDRQTGQMLQPTRLFDRTGQHLLYTFENPGIPRRFLPVDPALSGHFEPL
                     LVQYMVELYDPTFWQHPGFDWRSLTDPQPRTLAERLVSDLLLEQEPPSLQRALRMRLL
                     AAQVVSRYGRGQVLEWALNSTSYGHLTYGADSAARLYLGKPATDLSLAETALLLAVSQ
                     APALNPLDAPAAALENQQQVLALLHDRGLIPEADYAAAAAEALDLQPALEPANPVAVA
                     FSNLVINQLGAQFGSQRVERGGLTVITSLDYETQLQLQCALQTQLARLQG"
     gene            complement(13185..15074)
                     /locus_tag="PKL11_04355"
     CDS             complement(13185..15074)
                     /locus_tag="PKL11_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ12866.1"
                     /translation="MSSEETMFQEAMAAIEKGEKARARDLFTRLLKSDQNNPDYWLYL
                     SSVVETPKERIYCLKEVLRIDPEHVTARRGLIAMGAMPPDPSLAIPYRLQRRKWQADL
                     EVRQAEKLTGAARKQLIMYGAAGLVVLALLILAGIGVANINWRPTETNAAGFRVFPTA
                     VMSPTAGTVVPTPSPVGPTPPWGALQATYTPTPVYALTPHNLVEAYTIGRRAFERGDW
                     ASAITYFQQAVNSEPGAPDILYMLAESYRNSGQTTEALELYNQVIEDAPSFAPSYVAR
                     ARLYLAQDPPQFDRASTDLQKAIQLDAGYGEAYLELAHVKLVEGDPATALLQLDAAES
                     LTPASPLLYYYRGLAYLAQKKYADALEQADLALKLDIAMLPAYSLRGQAAYATEDYEQ
                     AVTDLTIYTTYGGEDLNALVMLARAHMANGDQDAALLAFDRALDINKKNVDVYLARAD
                     IYLADGEARKALADYKQALAYRPNSFEGRMGVARAYMALEQYGNAWEAYNDALDLTSQ
                     SLRSDIGRRALILYERGFAYWELGYPGNAIFDWEYILTLDKDEVDPEVWTGVEQALAE
                     HITATPTGKFTAGPTETLRPTPTSKNSTPAGTITPTPKASPTPKPSATPTPRVTPTRT
                     PTPKK"
     gene            15155..16114
                     /locus_tag="PKL11_04360"
     CDS             15155..16114
                     /locus_tag="PKL11_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNZ12867.1"
                     /translation="MLQILDAGFLTSVQDLGRTGHQQYGVPTGGPMDAFALRAANALT
                     GNPPEAAVLEIGLAGMRLRAESDLIIAVTGAGFEARIQGRRIPLWMSARLDAGQTLTL
                     TPVPGGCWGYVAVSGGVDTPPVLGSRATALNAGLGGLEGRTLRSGDRLACGPAQTGTH
                     AGLWLPPEYRPPYRTRPTLEAIPGPQYNDFTLEGQAALLFGEFAVRPDSDRMGYRLEG
                     PAVTHRGQAELLSEGLVMGAIQVPASGQPLVMMSERPTTGGYPKIAVIATADLPLLAQ
                     SPPGIGVVRFRLTEPEAAQARWRALLDGLARGVTEMDDVLTPI"
     gene            16135..16893
                     /locus_tag="PKL11_04365"
     CDS             16135..16893
                     /locus_tag="PKL11_04365"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNZ12868.1"
                     /translation="MDINCDMGESFGRYTLGQDAQIMPLVTSVSIACGFHAGDPLVMQ
                     GTVRLAVEQAKAIGAHPGYPDLQGFGRREMTLAPAEVEALVLYQIGALAGMLQAEGGE
                     LRHVKPHGALYNQAAKDPALAAAIARAVWRFDRELILVGLAGSALIEAGVEAGLRTAA
                     EGFPERGYNPDGTLIRRGQPGAQIESPKAAGEQAVRLATAGIQFLSGGEMHQVSVDTL
                     CIHGDAPQALQIARAVNAALSSAGVVIAGLGSEC"
     gene            complement(16890..17417)
                     /locus_tag="PKL11_04370"
     CDS             complement(16890..17417)
                     /locus_tag="PKL11_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12869.1"
                     /translation="MLKLSHLVGIPLKWTQPSMMRMQYELLTGETVAATLNFRSSWGT
                     FATAESADGCWTFKRIGFWQNRASIRVCGSETDLAIFTNNTWANGGTLEFVGGRSYKI
                     SNNFWMTKYEICNSAEEPVLRFNYGGFLHTSAEVEILPGAASLPELAPMLMFGFYIAI
                     MLHNDSAAAAAAASA"
BASE COUNT         3689 a         4800 c         5605 g         3456 t
ORIGIN      
        1 ccgcgaaggc ggcctcaccg tcggcgctgg cgtcattacc aaaattctgg actaacctgc
       61 ctgtcaggta gtaaggtaag caagcatggc ttcgaagaag aaagacatcc gcccggtgat
      121 cacctttgca tgcaatgaat gcaaggaacg caactacacc agcgagaaga atcgccgtaa
      181 tgatccgggc cgcatggaaa tcaagaaata ctgcccgcgg tgccacaaac atacgacgca
      241 ccgcgagact aagtaggttt cacctttctg cgccgggtgg ggttcacaag acccctcccg
      301 gtgcagaggt tgagcataca ggccagtagc tcaattggca gagcgccgct ctccaaaagc
      361 ggatgttgtg ggttcaattc ctgcctggcc tgcacaaaac ccaggcgacg ccgccttcaa
      421 ggcggcgttt tagccgtaaa aaaacaagga gcggaacgtg gtcaagacaa aggatacacc
      481 ctcagaaaag cctgttcaac cggttgcggc aaaatccaaa cctgttgaaa aaagtgcggc
      541 caaatccgac aagcccagca aacccaaccg catcaagcag tggtggcacg agacgatggg
      601 tgaactgcgc aaagtgagct ggcctacccg ccagcaagcc cagcgcctga ccgtcatcgt
      661 tctggtcacc atgcttgtca tggcattgtt cctgggtggg ctcgatttta tcttctctga
      721 actgattaca ctgctggtca agatctagac cgcggcaagt agaggtaata ccgatggatg
      781 cacacgacga tttcatgccc gaagaagaag gtattgagca gccgaaggag cgcaagcccc
      841 gccggtcaac cgggcgtgcg cggacatcgg agccggttca aaaccccgct tctgaagaaa
      901 ccgttaccga aagtaacgaa cgtgcctggt acgtcattca ctgctactca ggttatgaga
      961 acaaagttcg gcacaacctg gagcagcgga ttgagacgat gggaatgaaa gaccagatct
     1021 ttgatgtggt cattccaacc caggaagaaa ttgaagtcaa agacggcaag cgccgcacgg
     1081 ttgagcgcca tgtgttcccc ggctatgtgc tggtaaacat gctgctcagc gaagagtcgt
     1141 ggtatgtggt gcgcaacacc cccggtgtga cgggctttgt gggcatgggc aaccagccta
     1201 cccccctgcg ccccgaagag gtgcagcaga ttctgcgccg catggaggcc gaagcgccgc
     1261 atatcaaggt taccttccgg gtgggcgagc gggttcggat tgtagatggc ccgttcaatg
     1321 acttccgggg gaccgtctct gagattgata tggaacgcac caaggtgcgt gtgatggtca
     1381 gcttctttgg gcgtgaaacc ccggtggagc tggacttctt acaggttgaa aaagcgtagt
     1441 caacggactg cgtttaatca tgtgggaggg tgctcccctt atcacccgct ataaccacga
     1501 aggagaaaaa ctgtggcaaa gaaactgaaa gctgttgttc gtctgcagtt ggtggccggt
     1561 aaggccaacc ctgcaccgcc cgtcggtccg gccctggccg gctatggcct gaatattatg
     1621 gccttctgca aggaatacaa cgcccggacg caaaaccgca ccggcgatgt cattccggct
     1681 gaaatcagcg tctattccga tgggtctttt gtcttcatcc tcaagtcttc gccggcttca
     1741 gtattgctga agaaggctgc cggggttgac aagggttcgg ccacgccgaa ccggaccaag
     1801 gtcggcaaag tgacccgcgc tcaggtgcgc gagatcgccg agatcaagat gaaggacacc
     1861 aacgcggtgg atattgaaga tgccatgcgc caggtggaag gcaccgcccg caacatgggc
     1921 atcacggtcg ttgactaaag gaatgtggga gggtggccga acaggctgcc cgctggtacc
     1981 acgaaggagt attcatggca aagcacggta agaaatacga ggccgcaaag gccaaagtag
     2041 acagcaaaca gaactatgaa ccccgggccg ccgttgcgct ggcgaaagaa accgcttaca
     2101 ccagcttcga cgcgactgtg gaagttcaca tgcgcatggg tctggacccc cgccaggcgg
     2161 accagcaggt gcgtgatgtg gttgtactgc cgcacggact gggcaagaca gtgcgcgtgc
     2221 tggtcttcac ccagggtgaa ggtgtgctcc gcgcccagga aggcggcgcc gatcatatcg
     2281 ccgacagcga cgaatgggtt gccaaaatcc agggcggctg gacggatttc gatgtggcca
     2341 ttgccacgcc cgatatgatg ggcaaggttg gtaagctggg tcgtgtgctg ggtccgcgcg
     2401 gtttgatgcc gaaccccaaa gctggcacgg ttgttccggc tgaagatctg ccccgcgtta
     2461 ttgaagaagc caaggctggt cgtgtggaat tccggttgga caagaccgcc aacatccatg
     2521 tgccgattgg taaggcctct tttgatgaga ccaaactgtt cgaaaacttt gctgcgctga
     2581 tggaagcgat taagaaagcc cgcccggctg ctgccaaggg catctatatt cgccgcatca
     2641 ccctcgccgc caccatgggt cctggcattc acgtggatcc caatctggcg caaaatatca
     2701 gtctatagta aaataggggc gctggttaac cccggcgccc aaaaacaact taatttcacc
     2761 gtagaaagcc ggtgcctcag cgttctgagg ctcaatatcc tgccgaggtg gagacaaacc
     2821 ccaaaaaacc gcccccatgg ggcatggaag agtctttacc tggcaagtat gcaaggtaaa
     2881 gactttttaa taccctaaaa cagaaaggag gtgaatcctc attggctttc acaaaacagc
     2941 aaaaagataa gatggttgcc ctctaccgcg aatggctgcg caacgctcaa ggcgtggtgc
     3001 tggtcacctt caataaaatg tctatgaagg aaattgacac cctgcgcacc aagatccgcg
     3061 acgctggcgg cgaagcgcat gtggtgaaga acaccctgtt ctacaacgcc ctcagcgctg
     3121 aaggtatcca tgtggctgaa aagcccgaag gaacaaccct ggccgtattc tccagcgatc
     3181 cggcttccat tgccaaggca ctggttgaag cactgggtaa atcggaaatc ttccagatca
     3241 aaggtggttt cctgggtacg gagctgctca atgccgccca ggtcaaatcg ctggctgctc
     3301 tgccgcccct gcccgttatg cgcgcccgcc tgctgggtgt gctggtggcc ccggcgtctc
     3361 agctcgtccg cacgctggcc gaacccgccc gctcgatggc tgcagtcatc aaggcccatt
     3421 ccgaaaagga agccgttccg gcagccgggt agttctgtgt tgtccaacct ggcagggtcc
     3481 gccctgctga tcggagggca gcacgtctgc ccgtcatcaa gaaacaattt gattcaaaac
     3541 caataaatca agctaggaga tttttaaaaa tggctgatct gaccaaactc gttgaagaac
     3601 tgagcaagct ctctgttctg gaagctgccg atctggttaa aatgctcgaa gaaaagtggg
     3661 gcgtttccgc cgccgctcct gtggccgccg tggctgttgc cgctggcccc gccgccgccg
     3721 ccgaacccgt tgaagaaaag accgagttcg atgtcgtcct gaaggacgcc ggccccaaga
     3781 aaatcgacac catcaaagtc attcgccagc tcaccaacct gggcctggtg gaagccaaga
     3841 cgatggccga gaccgctggc gccaaagtcc tgaccgctgt cagcaaagaa gctgccaccg
     3901 atgccaaggg caaactggaa gccgccggcg cagttgtgga aattgcctaa tttctgaaca
     3961 acgcgttaaa caaaaagcgg gcgcaactgc gcccgctttt tgttttttta ttgtaataac
     4021 cgtcaggcgg ctacgctttg cacctggcga atgaccatga ccaccttgcc catgatgcgc
     4081 aggtgtgccg ggtcattcac aatgatgggc tgcatggtgc ggttagccgg ctgcagacga
     4141 atgcggtcgg gttcgcggta gaagtatttg agggtggttt cgtcccggtc atcgagccag
     4201 acggcgacca tttcgccgtt gttggcttcc tgggcagggc gcaggatgac gatatcacca
     4261 tcgttgacca tggcgtcgat cattgaatct ccctggactt ccagtgcgaa gagttgatca
     4321 atccgctcgc ggccgggcag catggaccgc gccacttcaa cgctgctttc ggggtcaaag
     4381 tagccggggg cgccgcccga ctgggtgggc atggggatgg gttcactggc aacaatgcgc
     4441 cccaggaggg ggacattgac caggtcgcgc aaggctgaac cagcctcacg cactgtctga
     4501 accaccttgc cgcctacggt gccgtgcacg ggctgcaaca cgcagatgct gcgcgagaca
     4561 cggccatcgc gccggatata gctgagctct tcgagttggc ggaggtagta atccaccagt
     4621 gaggtggact tgacatcaat gtgatcccca atctcgcgaa tggaaggtga atacccatgc
     4681 gattcctgga aatcggtcag gaactgcatg atacgggtct gtcgttcgct taatttctgg
     4741 ctacgtcggg ccattcgatg tgtcctttct caccggaaac acttgtacat ttgtgctaat
     4801 tatacaagaa caagtgttct gtgtcaaggc cattttgtca caattaatct tcgtcgcgtt
     4861 cccgcaggtg cgggaagagg atgacctctc ggatggtgga tcggtcggtg aggagcatga
     4921 ccaggcgatc tacgcccatg ccaaagccgc cgttgggggg catgcccatg gacatggcgc
     4981 gcaggtaatc ttcatccatg gggtggcgct gctcatcgtt atcgtcgtag gcccgcccca
     5041 tttcctggaa gcgcgcttcc tgatccagcg ggtcgttgag ttcggtgaag gcgttgcaca
     5101 gttccatgcc gcccacaaag ccctcaaagc gttccacagt ggtggggtca ccggggatgg
     5161 atttggccaa aggcgagata tcgcgcgggt agttatagag gaaggtgggt tggatgaggg
     5221 tcggctcgag aaaatcaccc agcaggctgt caatcagttt gccgcggggt gaccccggtt
     5281 tgaaggggac gttatgcgcc tgcatggctg cgccaaggct ttcagcggtg gggtgctggg
     5341 caatgtcgat gccggtggtc tcgatcagac cctgacggag atcaacccgc cgccaggggg
     5401 gggtgaggtc cacctggttg ccgcggaaat tgacggtacg ccgcccgagg acctggtcgc
     5461 aaacgaaggc gatcatttgc tcagtcatct ccatgatttt gagataatcg gcataggcca
     5521 tgtagaattc aagctgggtg aattcagggt tgtgtttata ggagacgccc tcattgcgga
     5581 agtcgcggcc aatttcatag acccggtcca ggccaccaac cagcaggcgt ttgagataga
     5641 gctcaaagga gatgcgcagg tagagctgct gatggagctg gttatggtgg gtcacaaagg
     5701 ggcgggcggc tgcgccgcca taaatgggct gaaggatggg ggtttcaact tccaggaagt
     5761 cacgctcatc caggaactgg cgcagggcgc gcacggtgtt ggcgcgggcg cggaagattt
     5821 cacgcacttc ggggttgact gccaggtcgg catagcgctg gcggtagcgg gtttcggggt
     5881 cggtcagacc ggcatggcgc accacctgtc catccaccag ttcgtctttg gcggccggca
     5941 ggggggtgat ggctttagcc agcatgcgga aagactgcac ctgcagggtg gcttcaccgg
     6001 tgcgggtgcg gaacatggtt ccttcagcct caataaagtc acccagatca aaggcctgct
     6061 gtaaaaagtc catggcttcg gggccaattt cgttaatgcg gaagaagagc tggatgcgcc
     6121 cgctgccatc ctcaatatgg gcaaaggcca ccttgcccat ggggcgcagg gcgcgcaacc
     6181 gcccggccag cgtggcggca acgggagtgg catcgccggc agcttcagca gcctcatagg
     6241 cggcgatgat ctgggcggcg gtgtgcgtca catgggcgcg ggttgggtag ggcagtatat
     6301 tttgatcgag caggcgttgt aatttttcca gtctgatttt ttcgagttct gtgcgttcca
     6361 tcaggtttga tcctttcaaa gaaaagcccc gcccctggat gcgggcgggg cggattgtcg
     6421 atttgtgggg ctattgcact ttcacaattt gcacgacaaa ctgtccggcc ggggcatcca
     6481 cctgcacccg gtcgccagcc ttatgattca agagggcgcg tcccaatggc gactcgttgg
     6541 agatgcggcc gcgggcgggg tctgcctcgg cagcgccgac gatggtatat tcctcatcag
     6601 aggtggtgcc ttcttcgcgg atgatcgccg tggagccgac ctggatggtg tcacccatgg
     6661 caggggcttc atcaatcaca tgggcagtgg cgagcagaat ttccagttcc ttgatgcggc
     6721 cttccacaaa ggcctgttcg tttttggcgg cttcgtattc agcgttctca atgagttcgc
     6781 cgccttccat ggcctcgtgc aagcgatctg ctatttcgtt acgcttggtg gtgcgaagaa
     6841 attccaattc ttcttgaagt ttctcgtagc cctcacgggt caagaatgtg ttatgcatgg
     6901 tcgtatcctg agtttaaatg tttgattccc aaagtaaaac ttgggagttg gaaaggccac
     6961 ttaagaaaaa acctctccag ttggagggat tctgtgtctt gcgatgtcct tgggttgtga
     7021 gtatgatatc acattaataa cggaaaggca agtacccgat taaaatcagc taaaataaaa
     7081 cgaagccccg gcgataccgg ggcttcgagc tgctgtaatg gcagtttgac taagctttgg
     7141 ccggttgagc gggtatctga gcgccttcac gcagcgcctg atagttcaac ggcaggaagc
     7201 ggtggtggcg ggcgggcagg tgttcctgca gggctttctc cagtgcttcc aggggcagca
     7261 ctttgaggtt ggccagcaga gcgcccatca agaccatgtt ggtcatgcgc ttgtcgccaa
     7321 gccgctcggc gatttcattg gcggggacca tgaccacaac gatgtcggtg cgtttcacgg
     7381 gccgattgat catggaggaa ttgactacca gcacgccgcc cggcttgatc acggattcgt
     7441 atttatcaag cgagggcagg ttcatggcca cagccgcatt cggatggcga accagggggg
     7501 agccaatttc ttcatcggcg atgaccaccg tgcagttggc cgtgccgccg cgcatttcgg
     7561 ggccgtagga ggggatccag gtgacctcga ggtcattatc catggctgcg taggagagga
     7621 gctgcccggc aaagagcacg ccctgaccgc caaacccgga gaagatgatt tcggtttgca
     7681 tgtcgtccct cttttagatc ttaatctcgg ccacagccgg gtggattttg tagtcgccca
     7741 acggatagta gggcaccatg ttcgcttcca gccacttcat tgcgtcttgc ggctgcatgc
     7801 cccagttggt ggggcaggtg gacagcattt caaccatcga gaagccgagg ccgcggagct
     7861 gggtttcaaa ggccatgcgg atggctttct tggccaggcg gatgttcttg gggtcgtgca
     7921 ggctgcggcg caccacatag ctggcgccat cgagggtgga aagcagttca gaagcgcgga
     7981 tgggataacc ttgcagttcc acatcacggc cattgggaga ggtggaggtc ttctgtccag
     8041 ggagcgtggt gggggccatc tgaccgcccg tcatgccgta attgctgtta ttaaggaaga
     8101 agacgctgat gttctcgccg cgggcggcag catgaacgat ttcgcccatg ccaatggagg
     8161 ccaggtcgcc atcgccctgg taggtcataa caacccggtc gggcatggcg cgcttgacac
     8221 ctgttgccat ggcgggggcc cggccatggg gcgcctggac gaagtcgaaa tcgaagtaat
     8281 tgtaggcaaa aaccgagcag cccacagagg ccacgccgat catgcgatcg cggatgccca
     8341 tttcatcaat gacttcggcg atcaggcggt gggcaacacc gtgcgtacag ccagggcagt
     8401 aatgcgtgga gcaatcgacc agcgcatcgg gatgttcata aacagtctgt tcgggagtgg
     8461 tttgggttac cattttatac ctcactcaat cacagtcgcc atacgcttga gccagcgatc
     8521 gcgcggatgc ccgtcggtgg gcagggggcc ggcagccatg cgccggatct cgcccaggat
     8581 ttcatccggg aaggggacaa tgccgcccag gcggccataa aattcaatcg gcttgcggcc
     8641 gcgcacgatg gagcggacat cttcgagcat ctgaccgcca ttcatttcgg tgaccagcag
     8701 gccatccact ttgtcaacga gttcgtccaa cactttggtg gggtagggcg ccagggtgat
     8761 ggggcgcagc aggccaaccg ggatgccttc agcacgggca gcgcgcaccg ccgagacggc
     8821 cacgcggcca gccgagccaa agccaactac cagatacttg gcgtcttcgg tgaagtaggt
     8881 cttgtagcgc acttcgttgg cttcaacttc ttgccagcga ttgagcatgc ggatgttggc
     8941 cacttcttct tcgggcgggt tcaggtagat agaggacagg attctgcggc cgcgctgacc
     9001 catcgtgccg gtggtggccc attcggcagt ggagacgccg cgcaggggag ccatgggggg
     9061 cagttcgcag ggttccatca tctggcccag gctgccatca agcagcacaa cggtgaccga
     9121 gcggtatttt tcggccaggt caaaggcaag cacagtgagg tcaacggctt cctggacggt
     9181 agcaggggcg aggacgatgg aatgataatc gccatgaccg ccgccatgca ccacctggtt
     9241 gtaatctgcc tgggcggggg caatgttgcc caggcccgga ccgccgcgca tcacatccac
     9301 cagcacaaca gggagttcgg tgccggcgat ataggagacg ccttcgagca tcaggctgaa
     9361 gcccgggctg gaggaagagc tcatcacacg ggtgccggtg caggctgcgc catagaccat
     9421 gttaatggcg cccagttcgc tttcggcctg gacaaaagca cggcccaatt cgggcatgcg
     9481 gcgagagagg tattccagca gttcggtctg gggggtgatg gggtagccaa aataggcacc
     9541 cagtccggcg cgcacagcag cttcagcaac ggcttcgttg cccttcatca attcacgagc
     9601 catattattc ctccgccttg atcggacggt taaccgtctc gcgataaacc gtaatggcgg
     9661 cttcggggca catcagcgcg cagatcccgc agccggtgca gccttctgcc accagggtgg
     9721 ccggatggta acctttgggg ttaatccctt ccgagagcgc aatcacatgc tgggggcagg
     9781 ccccaacaca aagattgcag cctttgcaag tcagttcctt gacttcgatt cgtccctttg
     9841 ggggcatcgc atcctcctgt gttgaacgtt tcctaaactc ggacaaaatc agtatgatat
     9901 tttgccaaat ctgattatgc cttctaaatc acagtgcggc tagtgtttat tgtcattcga
     9961 ggaagaaaca aatatcccct gatagatttt atgtatgaag gaatgcaagc caggggagat
    10021 tattcagaaa cgcgggtgaa gtacagggtg ccaaaaccga gatcgggtgg ggtttccacc
    10081 gacaaaacgc tgccatcgta gtcaaatgtg aaatcgtagg agggcaggcc aatggaaata
    10141 aagatgctga cgcgtttttc ggccacaaag acataggtgg cagagagacc ttcgccagtg
    10201 gcgcggtcaa ccagccagag ccgccctcct tcttcaaatc gcagctctac attgtagccg
    10261 ctggggcgcg ggctgtcggc ggtgagctgc cagcgcccaa ccaaaatctg ctcaaaaggg
    10321 gaatcaactg cggtgggcag ggtgccaaaa gatcccgcca gcagcaggcc acaactgacg
    10381 atcaggagca gtgcgccgat taagaccagg gccagggtga gtgggcggcg gcgggggttg
    10441 cgccagcccg ccagaaagcg gtcacgcaaa cggctcaggg cggggggcaa agcaagcagg
    10501 gggcgttcgg tttcagttgt cattcacggc tcacggtaaa tgaaatcgat tctgactcgc
    10561 ccaggttgcc agccagatcg taggctttga tgaccagggt atgccggccc ggtgctgctg
    10621 accagggcag gctgaaggcc atggcatcgc cagaggtaag gccttcgcca ccctgacgca
    10681 aacctgccag ggtgccatcc acccaccatt ccacccggtt tacacccacg gcttcctgta
    10741 cggcagcctg cagggtcaca atctgcccgg tggggggcgt gatggtttga tcggcaaggg
    10801 ggtaggggct ctcgaccagc ggggcggtgt tatccaccgt aacctgaagg atggccgaat
    10861 ccacctggga atcgtcgcgc acaacgacca gccggatggc gtataaccct tcgaggtcgg
    10921 tggtatccca ggcggccagt tcgccgccaa caaccatcga ttcgctggct tggcccacct
    10981 gcatccaact gcgggggttg atgccctgcc ccacctgcac ggtataggag gcaaaatcag
    11041 tgccggctgc ggtgccaata atgggcacca ccccgcgcac ataggcatac agccccggtg
    11101 aggtgacctg aacattgggc agtggaggcg gggcttgaat ggcatcatat tcaatgggcg
    11161 gcaggggcag gcccatccag cgggcccagg cctgtgcctc gggtggagcc atcagataga
    11221 cgcgtgcttc tacctgttcg ggtggggtga agacggtcgc caggcgcccc gtctcacggt
    11281 tgacctgaag ggactggtac agtgtgtcga gcatcaccgg ttcgctgccc gccataaaca
    11341 gttcgctgac cacattggga cagttgacgg ttggcagcat gccggatggg tcgcagacat
    11401 ccacagtttg caggccggag ggcatggtcc agttctcaac cggccggccg ccgtgtgtat
    11461 attgcatcag tgcgtgccag agcgatgcag agagatcgga gttgagggca atatcagaac
    11521 cggcccagac gactgccacg cgctgcggcg tataaccgac cgtccaggcc tgggtggcat
    11581 cggcaatctg ccccaggtgc gctccggccg ggcggccaat ttccagcagg ttggggtgcc
    11641 ccaggctggg ccagcgagcg gattcatcgc tcaggacgtg ggtgatcaaa taggccaggg
    11701 gctggctcaa gacgggctgg ctctcgctaa cctgctgatc gagcagggcg cggcccgagc
    11761 tgtcctgcac cagcaaaatc aagaccggct gcaggggctc atccactgcg gcgcggcggc
    11821 cgttcaacag gcccagggtg gcaaatgaac tgtacccctg tgcggcctgc aaaacagtca
    11881 gactgccgcc tgaggtcagc aggtcggcat cgggggcggc ggtggacagg cccgacaggc
    11941 ccagtgactc ggcggtacgc cagatgccca agacgccaat ctggtcggcc aggttgatga
    12001 ggggcactac ataatcgttt gccagagcca cgcgcagccg caccgggccn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcccctgaag gcgggcaagc tgggtttgca
    12181 gcgcacactg cagttgaagc tgggtttcgt aatccagcga tgtaatcacg gtaaggccgc
    12241 cgcgctccac ccgctgactg ccgaactgcg cacccaactg gttgatgacc agattggaaa
    12301 aagccaccgc taccggattg gcaggttcga gggctggctg caaatcaagt gcttcggcgg
    12361 cggccgcagc ataatcagct tcggggatca gaccgcggtc atgcagaagt gccagcacct
    12421 gctgttgatt ctccagggcg gcggcggggg cgtccaacgg gttgagcgct ggcgcctggc
    12481 tgacggccag gagcagggcg gtttcggcca gcgaaagatc agtggctggt ttgcccaggt
    12541 agagccgggc ggcgctgtcg gcgccataag tcaggtggcc gtacgatgtg ctgttcaagg
    12601 cccattccag cacctgaccg cgtccatagc ggctgaccac ctgggcggcc agcaggcgca
    12661 tgcgcagcgc ccgctgtaaa gaaggcggct cctgctccag cagtagatcg ctgaccagac
    12721 gctccgccag ggtacgcggc tggggatccg tcagggagcg ccagtcaaag ccggggtgct
    12781 gccagaaggt ggggtcgtaa agctcgacca tatactgcac cagaaggggt tcaaaatggc
    12841 cgctgagcgc cggatctact gggaggaagc ggcgcgggat gccagggttt tcaaaggtat
    12901 agagcaggtg ctgcccggtg cggtcaaaaa gccgggtggg ttggagcatc tgcccggtct
    12961 ggcgatcgag cagggtgggc aggatttgaa tggacggcag atcggcagtg agacgcgccc
    13021 aggcaaagcc cgcccagaca accaccgccg ccagtaacac acccaataca gcgcccaggg
    13081 cccgggcggt gcgtcccagg cggggtgtga attgatgacg ccgctgcaca cgccgccgcg
    13141 tccgacgagt aaccattaaa tgaagaggaa cagggcgcaa agcgttactt tttaggcgtg
    13201 ggggtacggg tgggagttac ccggggggtg ggcgtggcag agggtttagg cgtgggcgat
    13261 gccttgggcg tgggcgtgat ggtgccagcc ggggtgctgt ttttggaggt tggggtaggg
    13321 cgcagggttt cggtggggcc ggccgtaaat ttgcccgttg gcgtggcggt gatgtgttct
    13381 gccagggcct gctcaacccc ggtccagact tccgggtcca cctcgtcttt atccagggtc
    13441 aaaatatatt cccagtcaaa aatggcgttg ccaggatagc ccagttccca ataggcgaag
    13501 ccgcgctcgt acaaaatcaa cgcccggcgg ccaatatctg agcgcaggct ctgactggtc
    13561 aggtccaggg cgtcgttata ggcttcccag gcattgccgt actgctccag cgccatgtag
    13621 gcgcgggcca cgcccatgcg gccttcaaac gagttggggc ggtaggccag ggcctgcttg
    13681 taatcggcca gggccttgcg ggcttctcca tctgccaggt agatatcggc gcgggccaga
    13741 tacacatcca catttttctt gttgatatcc agggcgcggt caaaggccag cagggcggcg
    13801 tcttgatcgc cgttagccat gtgggcgcgg gccagcatca ccagggcgtt cagatcttcg
    13861 ccgccatagg tcgtataaat ggtcagatcg gtcacggcct gctcatagtc ttcagttgca
    13921 taagctgcct gaccgcgcag gctgtaggcc ggcagcatgg caatatccag cttcagcgcc
    13981 aggtcggcct gttcgagggc atcggcatat tttttttggg ccaggtaggc caggccgcga
    14041 tagtaataaa gcagcgggct ggcgggcgtc agactttcag cggcatccaa ctggaggagg
    14101 gctgtcgcag ggtcgccttc aaccagcttg acgtgtgcca gttccagata ggcctcgcca
    14161 tatccggcat caagctggat ggccttctgc aaatcggtgc tggcccggtc aaactgaggg
    14221 ggatcctgcg cgagataaag gcgcgcccgc gctacatagg acggggcaaa actgggggca
    14281 tcttcaatca cctgattgta gagctcaagc gcctcggtgg tttgccctga attgcggtag
    14341 gactcggcca gcatatacag aatatcgggt gcgccaggtt cgctgttgac cgcctgctgg
    14401 aagtaggtga tggcagacgc ccaatcgccg cgttcaaagg cacggcggcc aatggtgtag
    14461 gcttcgacca ggttgtgggg ggtcagcgcg taaacgggcg tgggggtata ggtggcctgc
    14521 agggcgcccc agggcggggt ggggccaacc ggtgacgggg ttggaaccac ggtaccggcg
    14581 gttggggaca tcaccgcggt gggaaagacg cgaaaaccgg cggcattggt ttcggtgggg
    14641 cgccagttaa tattggcaac gccaatcccg gccaggatca gcagggctaa aacaaccaga
    14701 ccggcagcgc cgtacataat aagctgtttg cgggcggcgc cggtcagctt ctcagcctgc
    14761 cggacttcaa gatcagcctg ccacttgcgc cgctgcaggc ggtagggaat cgccagtgag
    14821 gggtcagggg gcattgcgcc catggcaatc aagccgcggc gggcggtgac gtgctcggga
    14881 tcaatgcgca agacttcttt gagacagtag atgcgctctt tcggcgtttc aaccaccgag
    14941 ctgaggtaca gccagtaatc cgggttgttt tgatctgatt tcagcagccg ggtgaataga
    15001 tcgcgggcgc gggctttttc gcctttttca atggcggcca tggcctcttg aaacatggtc
    15061 tcttcagagc tcatgctatc agtttaacac aggcttcaaa tcaaggcaag cggatgaagg
    15121 cgaccagccg cgccgaatga tataatcgac gcggatgtta cagattctcg atgcgggatt
    15181 tttgaccagc gtgcaagacc tgggacgcac cgggcaccaa cagtatgggg tgcccaccgg
    15241 cggaccgatg gatgcatttg cgctgcgagc cgccaatgct ctgaccggca acccacccga
    15301 ggccgcggtt ttggaaattg ggctggctgg aatgcggctg cgggcggaga gtgacctgat
    15361 cattgccgtg accggtgcgg ggtttgaggc gcggatacag gggcggcgga tcccgctgtg
    15421 gatgtcggcc cgactggatg ccgggcaaac gctgacactg acgcccgtac cgggtggctg
    15481 ctggggttat gtggccgtct ctggcggggt ggatacgcca cccgtgctgg gcagccgcgc
    15541 cacggcattg aatgccggac tgggtgggtt ggagggccgc accctgcgca gcggcgaccg
    15601 gttggcctgc ggcccagcgc agaccgggac ccatgccggg ctgtggctgc cgccagaata
    15661 tcgcccgccc taccgcaccc gccccacgct ggaggccatc cccgggccgc agtacaatga
    15721 cttcacactg gagggacagg ccgcgctgtt atttggcgaa tttgccgtac ggcccgattc
    15781 ggatcggatg ggctatcggc tggaaggccc ggccgttacc caccgcgggc aggccgaact
    15841 gttgtctgaa gggctggtga tgggggcgat tcaggtgccg gccagcggtc agccgctggt
    15901 gatgatgagc gaacgcccga caacgggcgg ctaccctaaa attgcggtga ttgccacggc
    15961 agatttgccg ctgctggcac agtcgccgcc gggaatcggg gtggtgcgct tccgactgac
    16021 tgagcccgaa gccgcccagg cccgctggcg cgccctgctg gacgggctgg ctcgcggggt
    16081 tactgaaatg gatgacgtac tgacgccaat ttagaccttg aaggagcgta tctgatggac
    16141 atcaactgtg atatgggcga atcatttggc cgctacacgc tggggcaaga cgcgcaaata
    16201 atgccactgg tgacctcggt cagtattgcc tgcggcttcc atgcgggtga tccgctggtg
    16261 atgcagggaa cggtgcggct ggcagttgaa caggctaaag ccatcggagc gcatccgggc
    16321 tacccggact tgcagggttt tggacggcgc gagatgacgc tggcgcctgc tgaggttgag
    16381 gcgctggtgc tgtatcagat tggggcgctg gcggggatgc tgcaggccga gggcggcgaa
    16441 ctgcggcacg ttaaaccgca tggggcgctg tataaccagg cagccaaaga cccggcgttg
    16501 gcggcagcca ttgcccgcgc ggtatggcgg tttgaccgtg aactgattct ggtggggctg
    16561 gcaggctcgg cactgattga ggccggggtg gaagccgggc tgcgcaccgc ggccgagggc
    16621 ttcccggagc ggggctacaa cccggatggc accctgattc ggcgggggca gcccggcgcg
    16681 caaattgaat cacccaaagc cgccggtgaa caggcagtgc gcctggcaac ggcgggcatt
    16741 cagtttctga gcgggggcga aatgcatcag gtgagcgtgg acacgctgtg catccatggc
    16801 gacgcgcccc aggccttgca aatagcacgg gcggtcaacg cggcgctgtc cagcgcgggc
    16861 gtggtcatcg ctggcctggg atcggagtgc taagctgagg ccgcggccgc cgctgcggca
    16921 gaatcgttgt ggagcatgat ggcaatgtag aaaccgaaca tcagcatggg ggcgagttcg
    16981 ggcagggaag ccgcgcccgg cagaatctct acttcggcgg aggtatgcag gaagccgccg
    17041 tagttgaagc gcagcacggg ttcttcggcg ctgttacaaa tctcatattt ggtcatccag
    17101 aagttattgg agatcttgta gctgcgcccg cccacaaact ccagtgtgcc gccgttggcc
    17161 caggtgttat tggtgaaaat ggccagatcg gtttcagagc cgcagacgcg aatgctggcg
    17221 cggttctgcc agaaaccgat gcgtttgaag gtccagcagc catcggcgct ttcagccgtg
    17281 gcaaaggtac cccaggaact gcggaagttg agggtagcgg cgacggtttc gccggtaagc
    17341 agttcatact gcattcgcat catgctgggc tgggtccatt tgagcggaat gccaaccagg
    17401 tgagatagtt tgagcatttt ttcaatcctt tggcaaacaa aaagggttgc gtcatttcag
    17461 tataacatga tttttgaggc gggcagggcg gcagcacgag aagcgcaaaa tcagattttg
    17521 actacttgac aaaatagaat ttatgttcta ttataggaac agaataagcg ttctattgtt
    17581 tataaaggag tctccccatg gaattctcac ccacccgttc tgcttctgat ttcctgactg
    17641 gcgctttccg
//
LOCUS       DAOPVS010000029        16851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3468_length_16851_cov_48.278519, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000029 DAOPVS010000000
VERSION     DAOPVS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16851
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3468_length_16851_cov_48.278519"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..823)
                     /locus_tag="PKL11_04375"
     CDS             complement(128..823)
                     /locus_tag="PKL11_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12870.1"
                     /translation="MQKRTLWVIGFCLSLLALSACTWVGQPAALEEVISSADCTAPCW
                     RGITPGVTTFAEAQTLVQAMQDPASGAPTPAYVGVIETAQTLLVYFDQPEVNVDLRAD
                     SQGLISEISFNFEALKPGQRFKLGELIDRFGEPDALYVCHEQAEVRRAVVRVVYPQIE
                     MTFEQDLPSDETTFSVAYTKNTRLNTLTYYAPRQIPNCDFSSPWAGYGEVAFSLDAEA
                     EDSGTFCPLESKP"
     gene            complement(844..1686)
                     /locus_tag="PKL11_04380"
     CDS             complement(844..1686)
                     /locus_tag="PKL11_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12871.1"
                     /translation="MRLLSVLLRFRFVWLNLLFAAYLGWLQPVLLQRMVTAEQTHRPD
                     WLLGALLLGIQLFELAGLLLKRPASAFFARQNPDISQPNGCRENAKVAALVLVPVLHL
                     GAAAMLTLIAFDLWGWVGRAAATPGDWLLLPLFFVVLIKEAFFVVLMIGTGVGAAALA
                     PAEARFGARLNRWLSPPAGTRLTLKEILMDLAGDLLLLAFTALTYTAAWEFITAESPL
                     RHQGVERLGEYLGVSLLFFMVYFTTRSVYLMQEISIRQSRAARIFGWVSFGLVWAAAL
                     WGLG"
     gene            complement(1732..>2553)
                     /locus_tag="PKL11_04385"
     CDS             complement(1732..>2553)
                     /locus_tag="PKL11_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12872.1"
                     /translation="TPRPNGGETIELFLGGYSFRTLPDYDMDHDDRFVFMSPNDANAN
                     IVLSLAGSLADYGDTADSLLNEYLQSVFEEMDGSYTLAPVETMTLAGEAGIAYNYTGT
                     AMGNEVAGRTAIILLPDRRFVLGLGMAVQAANPTLWADRGAADFQTLLDSVELLEGGA
                     YLPTVTVGSSPCVIATDETYGYTEDNPIRVGGDAFGGPARERAFLDNLRGPNGEKLTY
                     LRLGSEPYGETILDIYTLTGLSVGIVTLYVDEYSYSDPQAPVGFTCAGDFPFIAP"
     assembly_gap    2554..2653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2953..3903
                     /locus_tag="PKL11_04390"
     CDS             2953..3903
                     /locus_tag="PKL11_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ12873.1"
                     /translation="MSTENEAILSAQSAEAHKEKNHAALTSVIAAVGLTAFKIVVGLL
                     TNSLGILAEAAHSALDLVAALVTFFAVRISGKPADPEHNYGHGKIENLSALFETLLLL
                     ATCVWIIYEAIQRLFFRTAEVELSIWAFIVMATSIVIDWGRSRVLYRAARKHNSQALE
                     ADALHFSTDIWSSSVVILGLVCVWLASKFSALAWLHSADAVAALGVALIVIYVSIELG
                     MRTIQGLLDTAPKGLAAKIDAAVAALPGIVNCHHTRLRQSGPNLFVDVHILVDGNLTL
                     AEAHELTERVEQAIQQIAPGADVTVHPEPAGTTEDVRFKL"
     gene            4041..4451
                     /locus_tag="PKL11_04395"
     CDS             4041..4451
                     /locus_tag="PKL11_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12874.1"
                     /translation="MKTRLLNILTVLLLVGAVIAAVVMLSIFNNPTNPINPFPPPTTA
                     PTLFVPSPTVTQTPIPPTYTPIPPTATFTLRPSSTLPPTITPYGMPTETPTFTITPTV
                     TATNTPMPTSSPTTLPTDTPVPTFTLPPTLTPTP"
     gene            4468..4788
                     /locus_tag="PKL11_04400"
     CDS             4468..4788
                     /locus_tag="PKL11_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12875.1"
                     /translation="MLDEISLELFEHLALLAALQLGPDETVYLHQQLNNQLRVIQALA
                     DIPLADETPITSHGVPFTAQTSPELRADIWEPCADAADILAQAPRLRDGYIVAPGIPH
                     TTLE"
     gene            4792..>6170
                     /locus_tag="PKL11_04405"
     CDS             4792..>6170
                     /locus_tag="PKL11_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNZ12876.1"
                     /translation="MELCDLSAYELALLLKQRKISAIETLDACLQRIEAVDGRPGALN
                     FSDPQPDDSRRVHAFITLTAERARAQALEIDRRLAAGEDPGPLAGVPFTVKDIFCVKD
                     TPTTAGSRILADFVAPYTATPVARLEAAGAVMLGKTNLDEFTYGSSGESSAFLPPTRN
                     PWDPRRVPGGSSGGSAASVAAGEAPLSLGTDTAGSIRQPAAFCGVVGLKPTYGRVSRH
                     GLIAFASSLDCPGPLARTVIDAALMLQVIAGPDSHDATAARVPVPDYISALEEGVRGL
                     RIGLSPDYFRITFPDPETGALELQPLPAEVEAAVRQAADRLAALGAEIIEDVPLPNTR
                     HGIPAYFVISRVEAASNLHRYDGLKYGYYDPARVDDVHKLYRKSRDAGFGIQPKLRIL
                     MGMYVSAAQYAEQYYTRALKVRTLIRRDFEQVFDPHGSYRLDALLTPTTPTPAFPMGA
                     LYGNSVLMQ"
     assembly_gap    6171..6270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6271..6536
                     /locus_tag="PKL11_04410"
     CDS             <6271..6536
                     /locus_tag="PKL11_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNZ12877.1"
                     /translation="TPAFPMGALYGNSVLMQYADQLTVPANHAGLPGLSLPGGLSADG
                     LPIGIQFLGPDFSEDRLLRLGRAYEQSTETEPWRAVRPKVLAG"
     gene            6606..7844
                     /locus_tag="PKL11_04415"
     CDS             6606..7844
                     /locus_tag="PKL11_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12878.1"
                     /translation="MTLTPNQLQNLIEAQSGWSGPTGGEESLLLPLLAQMDRELTRGL
                     WVEQTCAAGEIIFAEDAPGDAMYLIRSGMVAILKGSLEAPLVLGFRGPGEMIGEMALL
                     ENLPRSATVAALRETRLLRIEQEKFRQLLADHPALAFKVMAALSARLRSSDEARLDSS
                     RRGRRLVQKMDALQTENQQLQELQRVRAETSDLVVHDLRNPLNTIYLSLCTLELVLPP
                     ADLEANQELIDISKSAAERMKRLVNSLLDISRLEGNQDVLQRAVVRLPALVENIAAQM
                     RVTYKAGRVELRVELPADLPPVLIDEEKIGRVLANLLDNAIKYTPPEGHVTISARRQN
                     GQVAVMVTDTGSGIPAGERERVFERFAQVPGEGQKRRGFGLGLTFCRLTVEAHGGTIH
                     VEAGPNDVGSRFVFTVPAAN"
     gene            complement(7859..8641)
                     /locus_tag="PKL11_04420"
     CDS             complement(7859..8641)
                     /locus_tag="PKL11_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNZ12879.1"
                     /translation="MSHLKFLLAVWKANLQSAMEYRVSFLAQMFGMIANNTIYFLIWV
                     IFFDRFKDVRGWGLGDMYITFGIAASAVGLVSILFGNTFSLGDIITRGRLDYYLSLPR
                     PVLLHTISSRMISSGMGDFIYGFLSYAVSGSFSWDGLLRFVLAVMLAAVVFGAFLILV
                     QSLTFWIGSTNNLVQLATNAMVTFAIYPITLFDRMAKLILFTVIPAALIGTVPAEFTR
                     QFTWQTLALLLGGAAVFLALAIVVFQLGLRRYESGSAIQIEV"
     gene            complement(8648..9433)
                     /locus_tag="PKL11_04425"
     CDS             complement(8648..9433)
                     /locus_tag="PKL11_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNZ12880.1"
                     /translation="MKKYWAIFKLQLANGLAYPGEFIGRSLLILPFMWIFFQLWRVTF
                     EASGGAEINGLTLRSTLWYLVIAEVIELSRPRVANTISEAVKDGSIAYILSKPYNFLW
                     YHYSSSLADTIFRALLNLVLGGGVAWLLVGPPPSLWGLLAVLPLLMGAWALNYCIAAL
                     IGLLAFWVEDTSAFQWIYQKLAFILGGLLIPLDFYPAWLRSIADVLPFSAVTYAPARL
                     FVEPSLNGFLAALGQQALWLLVFAGGLGLLYRRSVTVLTVNGG"
     gene            complement(9430..10431)
                     /locus_tag="PKL11_04430"
     CDS             complement(9430..10431)
                     /locus_tag="PKL11_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ12881.1"
                     /translation="MAAITVRELRKTFQTKHKAAGLRGSFRSLFRPQYATVEAVRGIS
                     FEMEPGELLGFIGPNGAGKSTTIKMLTGILHPTAGEADVLGYCPWRERQKLAHHIGTV
                     FGQRPQLWYHLPAMDTFTLFGKIYDLDDAETRRRIAFLAEAFEIQDLLDTPVRKLSLG
                     QRMRCEVAASLLHRPRLLLLDEPSIGLDVVAKQHIRDAIRTMSREEGVGVLLTSHDAG
                     DLEALCHRVIIVHHGQIVYQDKVSNLKRKFLTRKTVEVRYAEEVAKDFHLEGMQTLKT
                     GRYGVKLEFDTQRTPADAVLARLSAAGTLVDITISDPPLEEVIARIYESAASEVDGQ"
     gene            10638..10994
                     /locus_tag="PKL11_04435"
     CDS             10638..10994
                     /locus_tag="PKL11_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNZ12882.1"
                     /translation="MTFDPSTPPPAPVEMPSISQPPAPAEKTWAAITALVLGIINMCG
                     WLLPICGGPIGIAAVIFGILGLKTSKRTMAIIGLVLGGLTLILTIINSVAGAVLFANG
                     GFDPSQFDPSQWLPQN"
     gene            11383..11892
                     /locus_tag="PKL11_04440"
     CDS             11383..11892
                     /locus_tag="PKL11_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12883.1"
                     /translation="MTRISVIGNAGGGKSTMCRKLSKALNIPLYPIDKIQWKPGWTPA
                     SYDEIKAQHDHILAQERWIIDGWGNWDLIETRFAASDTIIFVDHPLLVHYWWALKRQF
                     ACIFAPRVDGPEDCPMLPMTLPLLKMIWNIHYHSRPRLLELVNRFRADKQVIHITSPK
                     ELRLFMAKY"
     gene            12071..13129
                     /locus_tag="PKL11_04445"
     CDS             12071..13129
                     /locus_tag="PKL11_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HNZ12884.1"
                     /translation="MDWQIAGHDWAARLLQQQLARGETRHAYLFTGPPGVGRRTLALR
                     LAQALNCLQPPEPGQFCGKCRACQQTAAMQFPDLTVVQSAQVGDELKIDAIRALSHTL
                     SLAPYAARWRVALLLRFHEANANAQNALLKTLEEAPPSAILLLTADSAEDLLPTISSR
                     CEVVRLRPLGLDALTAYLTQNHSLGAAEARQLAQISAGRVGLAEQYLADPQAVQARVE
                     QLDELWQMLCAPHRERFLYAKKIAPPSSKQERSLTRAALRGRFELWLSFWRDVLLQVN
                     GAAAPLSNPDRAAQIAAVVAVVTPTQALRQVTALEQAIDRLPGANLQLLVEVLLLDWP
                     RLPGGSALASLPEMVAED"
     gene            complement(13144..16470)
                     /locus_tag="PKL11_04450"
     CDS             complement(13144..16470)
                     /locus_tag="PKL11_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ12885.1"
                     /translation="MSDIETIQQAMAALEAQRALLGDTAVDAALTGLRQKLAELQAQA
                     APAAQRKQVTILFADVSGFTALSETLDAEEVHDLMNALWERLDALILQRGGYIDKHMG
                     DAVMAIWGVHTAREDDAEQCVRTALEMQTELNSLTTDWPHPVRMRVGVHTGPVLLGLV
                     GTTSEFTATGDAVNLTSRLQNAAPVGGVIISHDAYRQVRGIFDVQPQEPITLKGKREP
                     VQTYIVQRARPRSFRMATRGVEGVQTPMIGREAELAALQQAFESAMHQAQARLVSIVG
                     EAGIGKSRLLIEFESWLDALPERVYLFQGRATPEMQSIPYGILRSLFSNRFDILESDR
                     PATVRAKFEAGMAGSLPPDQIALVGQLVGFDFSGTAAVQNLLGSPSFARLAQAYLVTY
                     FRALASSGALAVFMEDIHWADASSLDLLENLTASLSDQRLMIICLTRPILFERREDWS
                     RGPAALRIDLQPLSPEMSRRLVGEILHKVQNLPEPLRELIVQNAEGNPFYVEELIKTF
                     IENGVILRGEDHWQVQVEQLQRAHVPATLTELLQARLDGLPRDERDLLQHAAVVGQTF
                     WDATLKAMHAPGDTVSDTASLLAKLQWRELIFQQMRSTFAGAQENTFKHSMLREAAYE
                     TVLLKLRRIYHRQVAEWMEDSAGERLPEFAAVIARHYELAGEPARAAAWLRRLGDDAL
                     RTGAAPESIAAFEHALTLLPPDHLNERAALQIRLGNALERTGDYDPAAEHLQQALADS
                     QHLQAHDLMVEATLGLGRVLMRQGDYDEATRLNQAVLAQTQDPLLAAQAERVLGVIAY
                     YQAEYISAIEHITASLNLESQNSNRNGMATCLSNLGLVAFSQGDYPAAIRYFEQSLVI
                     HREIGDRSGISAALGNLGMVYHDLADVVSARQAHTESLQIAREIGDRRGECSSLNSLG
                     SVALTLNDLNTALQYFESALALARQVDDPMLSVRILISLGWASLLHQDYAASRRYSEE
                     SLALAEELGDRESVISCLTNLGHAAWPEGDWAVASGYYRRSIVEAQEIGAPPRLLEAL
                     AGLAGCYWAGGQPERAAELLGLALNHPATNSDVTQAADFVLPHLQAALPAAGLQAALA
                     RGQTLDLEATVESVLAESRG"
BASE COUNT         3326 a         5266 c         4947 g         3112 t
ORIGIN      
        1 ctttgcgcct tggcgttaac ttcatctcag ggcgcccagc ctgttcaggc tgcacacaca
       61 taagtgcgcc cctacatcaa cctgaaggcc aaatcctaaa tcccaaatct aaaatctgaa
      121 attcccctca gggtttgctt tccagcgggc agaaggtgcc cgagtcttcg gcctcggcat
      181 ccagcgagaa ggccacctcg ccgtagccgg cccaggggga cgaaaaatca cagttgggga
      241 tctggcgggg ggcgtaatag gtcagggtgt tcaggcgggt gtttttggtg taggccacgc
      301 tgaaggtcgt ctcgtctgag ggcagatcct gctcaaacgt catctcaatc tgcggataga
      361 ccacccgcac cacggcccgg cgcacctcgg cctgttcgtg gcagacgtac agcgcatccg
      421 gttcgccaaa gcggtcaatc agctcgccca gcttgaaccg ctggcccggt ttgagcgcct
      481 cgaaattaaa gctgatttcg ctgatgaggc cctggctgtc ggcgcgaaga tccacattga
      541 cctcgggctg gtcgaaatag acgagcaggg tctgggcagt ctcaatcacg cccacatagg
      601 cgggcgtggg ggcgccgctg gcggggtctt gcatggcctg gaccagggtt tgcgcctcgg
      661 caaaggtggt gacgcccggc gtgatgccgc gccagcaggg cgcggtgcag tcggcggagc
      721 tgatgacttc ttccagggcg gcaggctgcc ccacccaggt gcaggcgctc agcgccagca
      781 ggctcagaca gaaaccgatg acccagagcg ttcttttttg catttttatc acctctttgt
      841 taatcaacct aaaccccaca gcgctgccgc ccagaccagc ccaaagctga cccagccaaa
      901 gatccgtgct gcccggcttt gccggatgct gatttcctgc atcagataga cggagcgggt
      961 ggtgaagtac accatgaaaa acagcagact gacgcccagg tattccccca ggcgctccac
     1021 cccctgatgg cgcagcggcg actcggcggt gatgaactcc caggcggcgg tgtaggtcag
     1081 ggcggtaaag gccagcagca gcaggtcgcc ggccaggtcc atcaatattt ctttgagcgt
     1141 cagccgggtt ccggcgggcg gcgacagcca gcggttcagt cgggcgccaa agcgcgcctc
     1201 ggcgggggcc agggcggccg caccgacgcc ggtgccgatc atcagcacca caaagaaggc
     1261 ctctttgatc agcaccacaa agaacagcgg cagcaacagc caatcgccgg gcgtggctgc
     1321 agcgcggccc acccagcccc acaggtcaaa ggcaatcagg gtcagcatgg cggcagcgcc
     1381 caggtgcagc accggcacca gcaccagggc cgcgaccttt gcattctccc ggcagccgtt
     1441 gggttggctg atgtcggggt tttgccgggc aaagaaggcg ctggcgggcc gcttgagcag
     1501 cagcccggcc agttcaaaca attgaatacc cagcagcagc gcacccagca gccaatcggg
     1561 gcggtgcgtc tgttcggcgg tgaccatgcg ctgcaggagg accggctgca gccaccccag
     1621 gtaggcggcg aacagcaggt tgagccagac gaagcgaaaa cgcagcagca cagacagcag
     1681 tcgcatgctg cgattatatc aggcctgaag cctgccgccc gcccaaaccg cctacggtgc
     1741 tatgaagggg aaatctccgg cgcaggtgaa gccaaccggc gcctgggggt cagagtagct
     1801 gtactcgtcc acatacagcg ttacaatgcc aacgctcagt ccggtgaggg tgtaaatatc
     1861 gaggatggtc tcgccgtaag gctcagagcc cagccggaga taggtcagct tttcgccatt
     1921 ggggccgcgc agattatcca aaaaggcgcg ttcgcgcgcc gggccgccaa aggcatcgcc
     1981 gcccacccgg atggggttat cctcggtgta gccgtaggtc tcgtcggtgg cgatcacgca
     2041 ggggctggag ccgacggtga ccgtgggcag gtaggctccg ccttcaagca gttcaaccga
     2101 atccagcagg gtttgaaaat cggctgcgcc gcggtcggcc cagagcgtgg ggttggcagc
     2161 ctgtacagcc atgcccaggc caagcacaaa acgccggtct ggcagcagga tgatagccgt
     2221 gcgcccggca acctcgttgc ccatggcggt gccggtgtag ttataagcaa tgccagcctc
     2281 tccggccagg gtcatggtct ctaccggggc gagggtataa ctgccatcca tctcctcaaa
     2341 caccgactgg aggtactcgt tgagcagcga atcggcagta tcgccatagt cagccaggct
     2401 cccggccaga gacagcacaa tgttggcatt ggcatcattt ggcgacatga acacaaagcg
     2461 atcatcatga tccatgtcgt aatccggcag ggtgcggaag gagtagccgc ccaggaacaa
     2521 ctcaatggtt tcgccgccgt tgggccgggg ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnntcggtgg gcaccggcgt ggccgtgggc gcgggggttt cggtgggcgc
     2701 caggcggggc atcagcccgc aggccaggct gaccagcagg atgagcggta taagaaagag
     2761 caggcgggtg tgttttttca tcgtttcctt ccctgaggtg aaatgttact gtcattatac
     2821 tgagttttgc cgcgccccac tgccgcttgc aatcccgagt aaagtcacct gccgggatga
     2881 catttggtat tatgccgccc tttcaaacgg tatagaatga gatattatct atctgacgtc
     2941 aggaaaatat ttatgtccac tgaaaatgaa gccatcctct cagcccagag cgctgaagcg
     3001 cataaagaaa aaaatcacgc cgcgctgacc tcggtgattg ccgctgtcgg cctgacggcc
     3061 ttcaagatcg tggtcggcct gctgaccaac agcctgggca ttttggccga ggcggcccac
     3121 tctgcgctgg atctggtggc cgcgctggtc acctttttcg cagtgcgcat ttcgggcaag
     3181 cctgccgacc ccgaacacaa ctacgggcat ggcaaaattg aaaatctctc agccctgttt
     3241 gaaaccctgc tgctgctggc cacctgtgtc tggattattt acgaggccat ccagcggctc
     3301 ttcttccgca ctgccgaggt ggagctgtcg atctgggcct ttattgtgat ggccacttca
     3361 attgtgattg actggggccg ttcccgggtg ctgtaccgcg cggcccgcaa gcacaacagc
     3421 caggcgctgg aggccgatgc cctgcacttc agcaccgaca tctggtcatc cagcgtggtc
     3481 atcctgggcc tggtgtgcgt ctggctggcg agcaagtttt ctgccctggc ctggctgcac
     3541 agtgccgatg ccgtggcggc cctgggtgtg gcgctcattg tgatttacgt cagcattgag
     3601 ctgggcatgc gcaccattca aggcctgctg gataccgccc ccaagggcct ggccgcgaag
     3661 attgatgcgg ctgtggcggc gctccccgga atcgtcaact gccaccatac ccgcctgcgc
     3721 caatctggcc ccaacctgtt tgtggatgtg catattctgg tggatggcaa tctgaccctg
     3781 gccgaagccc acgagctgac cgagcgggtg gagcaggcca ttcagcaaat tgcgcccggc
     3841 gccgatgtga ccgtgcaccc cgaaccggct ggcacaactg aagacgtgcg ctttaagctt
     3901 taataatgta gatgctggtc agggagtgtg acggctcatt aaagcgaatt taatcattcc
     3961 tattcccaaa acaccgcaac ctgggatata ctgacatcag gatatccccc atttcggggg
     4021 gaaaggatgg ctgctttcag atgaaaaccc ggcttttgaa tatcctgacg gttttgctgc
     4081 tggttggcgc agtgattgcc gccgtggtga tgctttcgat attcaacaac cccaccaatc
     4141 ccatcaatcc cttcccgccg cctaccaccg cgccaacgct gtttgtcccc agccccacgg
     4201 tgacccagac ccccatcccg cccacctata cccccattcc gccaaccgcc accttcacgc
     4261 tgcggcccag ttccaccctg ccgcccacca tcacgcccta cggcatgccc accgagacgc
     4321 ccaccttcac catcaccccc acggtcactg ccaccaacac ccccatgccc accagcagcc
     4381 ccacaacgct gcccaccgac acccccgtgc ctaccttcac cctgccgcca accctgaccc
     4441 ccacgccctg ataaggaccg gccgcccatg ctggatgaaa tttcgctgga attgtttgaa
     4501 cacctggccc tgctggccgc gctgcagctt ggccccgatg aaaccgtgta tttgcaccag
     4561 cagctcaaca accagctccg ggtcattcag gcgctggcag atattcccct ggcagatgag
     4621 acgcccatca cctcgcacgg cgtgcccttc accgcccaaa ccagccccga gctccgggcc
     4681 gatatctggg agccgtgcgc cgatgcggcg gatattctgg cgcaggcccc ccggctgcgg
     4741 gatggctata tcgttgcgcc aggcattccg cacaccaccc tggaatagcc gatggaactt
     4801 tgcgatcttt cggcttacga gctggcgctg ctgctcaaac agcggaaaat ctccgccatc
     4861 gaaacgctcg atgcatgcct ccagcgcatc gaagcggtgg atggccgccc cggtgcgctg
     4921 aatttcagcg acccccagcc cgatgattcc cgccgcgtgc atgccttcat caccctgacc
     4981 gccgagcgcg cccgcgccca ggccctggaa attgaccgcc gcctggctgc cggtgaagat
     5041 cccggcccgc tggccggggt gcctttcacg gtcaaggata ttttctgcgt caaagatacc
     5101 cccaccaccg ccggttcgcg catcctggct gattttgtgg cgccctacac cgccaccccc
     5161 gtggcccggc tggaggccgc cggcgcggtg atgctcggca agaccaacct ggatgaattt
     5221 acctacggct catccggcga gtcttcggcc tttctgccgc ccacccgcaa cccctgggat
     5281 ccgcgccggg tgccgggcgg gtcgtccggc ggcagtgcgg cttcggtggc cgcgggcgag
     5341 gccccgcttt cgctgggcac cgacaccgcc gggtccatcc ggcagccggc ggccttctgc
     5401 ggcgtggtcg gtctcaagcc cacctacggg cgcgtctcgc gccacggtct gatcgccttt
     5461 gcctcctcgc tcgactgccc cggcccgctg gcgcgcaccg tgatagatgc cgccttgatg
     5521 ctccaggtca ttgccgggcc agactcgcac gatgccaccg ccgcccgcgt ccccgtgccc
     5581 gattacatca gcgcactgga agaaggcgtg cgcggcctgc gcattggcct atcgccagac
     5641 tatttccgca tcaccttccc cgaccccgaa accggcgcgc tggaacttca acccctgccc
     5701 gccgaggttg aggccgccgt gcgccaggcc gccgaccggc tggcagccct gggcgccgaa
     5761 atcattgagg atgtgcccct gcccaacacc cgccacggca tcccggccta ttttgtcatc
     5821 agccgggtag aggctgcctc gaacctgcac cgctacgacg gcctgaagta cggctattat
     5881 gacccggcgc gggtggatga tgttcacaag ctctaccgca aaagccgcga tgccgggttt
     5941 ggcattcaac ccaaactgcg cattctgatg ggcatgtacg tctcggcggc gcagtatgcc
     6001 gagcagtact acacccgcgc cctcaaagtg cgcaccctca tccggcgtga ctttgagcag
     6061 gttttcgatc cccacggcag ctaccgcctg gatgccctgc tgacccccac cacgcccacc
     6121 ccggccttcc ccatgggcgc gctctacggc aactcggtgc tgatgcagta nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccaccccggc cttccccatg ggcgcgctct
     6301 acggcaactc ggtgctgatg cagtacgccg accaactgac cgtgcccgcc aaccatgccg
     6361 gcctgccggg gctctcactg ccaggcggcc tctcggcgga cggcctgccc atcggcatcc
     6421 agttcctcgg tcccgatttt tctgaagacc gcctcctgcg cctgggccgc gcctacgaac
     6481 aatctaccga gaccgaaccc tggcgcgctg tgcgcccgaa agtgctggca ggctgaatcc
     6541 cgcccgccgc ctgtactaaa cacctgctcc aaaaatctca aaatcctaaa tccccgcgag
     6601 gccccatgac cctcaccccc aaccaactgc aaaacctgat cgaagcccag tccggctgga
     6661 gcggccccac cggcggcgaa gaatcgctgc tgctgccgct gctggcgcag atggaccgtg
     6721 aactgacccg cggtttgtgg gttgaacaaa cctgcgccgc gggcgaaatc atctttgccg
     6781 aagacgcccc cggcgacgcc atgtacctga tccgctcggg catggtggcc atcctcaagg
     6841 gcagcctgga ggctcctctg gtgctgggct ttcgcggccc cggcgagatg atcggcgaga
     6901 tggcgctgct ggagaacctg ccgcgctcgg ccaccgtggc cgccctgcgc gagacccgcc
     6961 tgctgcgcat tgagcaggaa aaattccgcc agctcctggc agatcatccg gcgctggcct
     7021 tcaaggtgat ggccgcgctc agcgcccgcc tgcgctcatc agacgaggcc cggctggaca
     7081 gcagccgccg cggccggcgg ttggttcaaa aaatggatgc gctgcaaacc gaaaaccagc
     7141 agcttcaaga actgcagcgg gtgcgggccg agacctcgga tctggtggtg catgacctgc
     7201 gcaatccact caacaccatc tacctgtcgc tttgtacgct ggaactggtg ctgccgcctg
     7261 ccgacctgga ggccaaccag gaactgattg atatctccaa atcagcggct gagcggatga
     7321 agcggctggt caattcgctg ctggatatct cgcgcctgga gggcaatcag gatgtgctgc
     7381 agcgcgccgt ggtgcggctg ccggcgctgg tggaaaacat tgccgcgcag atgcgcgtca
     7441 cctataaagc cgggcgggtg gagctgcggg tggaactgcc cgccgacctg ccccccgtgt
     7501 tgattgacga ggaaaagatt ggccgcgtgc tggccaactt gctggataat gccatcaaat
     7561 ataccccgcc cgagggccat gtgaccatct cggcgcggcg gcaaaatggc caggtggcgg
     7621 tgatggtcac cgacacgggg tcgggcatcc ccgcgggcga acgcgagcgt gtgttcgagc
     7681 ggtttgccca ggtgccgggc gagggtcaga agcggcgcgg ctttgggttg gggctcacct
     7741 tctgccgcct gacggtagaa gcccacggcg gcacgatcca tgtggaagcc gggccaaatg
     7801 atgttggcag ccggttcgtc ttcaccgtgc ccgccgccaa ctgaataaga tcacggcgct
     7861 aaacttcgat ctgaatggcg ctgccgctct cgtagcgccg caaacccaac tgaaagacca
     7921 caatcgccag cgccaggaac accgcagccc cccccagcag caaggccagc gtttgccagg
     7981 tgaactggcg ggtaaattcg gccggcacgg tgccaatcaa cgccgccggg atgaccgtga
     8041 acaggatcag cttggccatc cggtcgaaca gggtgatggg gtaaatggca aaggtgacca
     8101 tggcgttggt ggccaactgc accaggttgt tggtgctgcc aatccagaac gtcaggcttt
     8161 gcaccaggat caggaaggcg ccaaagacca ccgccgccag catcactgca agcacaaacc
     8221 gcagcagccc gtcccatgag aaagaccccg aaaccgcata gctcaaaaag ccatagatga
     8281 aatctcccat cccgctggag atcatccggc tggagatggt atgcagcagc accggccgcg
     8341 gcagcgaaag gtagtaatcc agccgcccgc gggtgatgat atcgcccagc gaaaaggtat
     8401 tgccgaacaa aatcgaaacc agcccaacgg cgctggcggc aatcccaaag gtgatgtaca
     8461 tatcccccag gccccagccg cgcacgtctt tgaaacggtc aaagaaaatc acccagatca
     8521 gaaaatagat ggtgttattg gcaatcatgc cgaacatttg cgccaaaaac gagacccggt
     8581 actccatggc agactgcagg ttggctttcc agacggcgag tagaaatttt aagtggctca
     8641 ttgaccctta ccctccattc accgtcagaa cggtcacgct gcggcggtag agcaggccca
     8701 gcccgccagc aaagaccaac agccacaggg cctgctggcc cagggcagcc agaaaaccgt
     8761 tcaggctggg ctcaacaaac agccgcgccg gggcatacgt cacggcggaa aacggcagca
     8821 catcggcaat gctccgcagc caggcagggt aaaagtccag cgggatcaac agcccgccca
     8881 ggatgaaggc cagtttttga taaatccatt gaaaagccga ggtatcttcc acccagaacg
     8941 ccagcagacc aatcagcgcg gcgatgcagt aattcaaggc ccaggcgccc atcagcagcg
     9001 gcagcaccgc cagcagcccc cacaggctgg gcggcgggcc taccagcagc caggccaccc
     9061 cgccccccag caccaggttg agcagtgccc ggaaaatcgt atctgccagg gatgaactgt
     9121 agtgatacca gagaaagtta tagggcttgc tgaggatata ggcgatagac ccgtctttga
     9181 cggcctctga aatcgtattg gccacccgcg ggcggctcaa ttcaatcact tcggcaatca
     9241 ccaggtacca cagggtgctg cgcagcgtca gcccgttgat ctcggcgccg cccgaggcct
     9301 caaaggtgac gcgccacaac tggaagaaga tccacatgaa cggcagaatg agcaaactgc
     9361 gcccgataaa ttcacccgga taggccagtc cgtttgccag ttgaagttta aagatggccc
     9421 agtacttttt cattgcccat ccacctcgct ggcggcgctt tcgtaaattc gggcaatcac
     9481 ctcttccaat ggcgggtctg agatggtgat atcgaccagc gtgccagccg ccgagagccg
     9541 cgccagcacg gcatcggcgg gggtgcgctg ggtatcaaat tccaacttga cgccgtagcg
     9601 cccggttttg agggtttgca taccttccag gtgaaagtct ttagccacct cttcggcata
     9661 gcgcacttca actgtcttgc gggtcaggaa cttacgcttc aaattggaga ccttatcctg
     9721 atagacaatc tgcccgtgat gcacaatgat gacgcgatgg cagagggcct ccaggtcgcc
     9781 ggcgtcgtga gaggtcagca gcacgccaac gccttcctca cggctcatgg tgcgaatggc
     9841 atcgcggatg tgctgcttgg caaccacatc caggccaatg gagggttcat ccagcagcag
     9901 aaggcggggg cggtgcagca gcgatgccgc cacctcgcag cgcatacgct gcccgagcga
     9961 gagcttgcgg acgggcgtat ccagcaggtc ttgaatctca aacgcctcag ccagaaaggc
    10021 aatccgccgc cgggtttcgg catcgtccag gtcatagatc ttgccgaaca gggtgaaggt
    10081 atccattgcc ggcaggtgat accacaactg cgggcgctga ccaaagactg tgccgatgtg
    10141 gtgggccagc ttctgacgct cgcgccaggg gcagtaaccc agcacgtcgg cttcgccggc
    10201 cgtggggtgc aggatgccgg tgagcatttt gatggtggtg gatttgcccg ccccgttggg
    10261 cccgatgaac cccagcagct cgcccggttc catctcaaac gaaatgccgc gcacggcttc
    10321 aacagtggca tactggggcc ggaacagcga gcggaagctg cccctcagcc cggcggcctt
    10381 gtgtttggtc tggaatgtct tacgcagctc gcgcacggtg attgctgcca tggtcaacct
    10441 cgcagtgtgg aatgaaaaat gatggcctta agcatatcac agttcacggt gcacggggcg
    10501 ccgggctcca cagtaaaaat atctcccgcc agaatgcccc tcgagcccga cagaatcttc
    10561 tccgccggcg aattaaacct gtctgagttg tgctataatc cagacagcgt attttttatt
    10621 tccccaagga gttaaaaatg actttcgacc cctcaacccc tccgcctgcc ccggtagaaa
    10681 tgccgtctat ctcgcagccc cccgcccccg ctgaaaagac ctgggcggcc atcacggcac
    10741 tggtgctggg catcatcaat atgtgcggct ggctgctgcc gatttgcggc ggccccattg
    10801 gtattgctgc cgtcatcttc ggtatcctgg gcctcaagac ctccaagcgc accatggcca
    10861 tcattggcct ggtcctcggc ggcctgaccc tgatcctgac gatcatcaat tctgtggccg
    10921 gtgcggtgct gtttgccaac ggtggtttcg atccttcgca gttcgatccc tcgcagtggc
    10981 tgccgcagaa ctaatcacgg ttcgccccct tgcgcccggg ttgctccggg cgcaagtctt
    11041 ttaaggggga tcagcacaac ctaacccggg gcaatcacac taaatttttt accgccgtat
    11101 atgactttag tgcaattacc ctggtcatga atgtttgcct ttacaggcta agtgtataat
    11161 atcttaagat ttgtcagtag ttgatcgcct ggtgtagcca ggcatcattg caggacaacc
    11221 tacaatattc atctggttat acggaataat ggcagcgcat tgtcagtccg tctaattaat
    11281 agttgggccg ttgccgcaag gcaatatcaa cacaaaccca aaaccgtatt gaaggcttcc
    11341 cgacattatt cacaataaac gatataaaac aggcaaagag ctatgacgcg tatttcggta
    11401 ataggaaatg ctggcggcgg taaatcaacc atgtgtagga aattaagcaa agcccttaat
    11461 attcctttat atcccatcga taagattcaa tggaagccag gatggacacc ggcttcttac
    11521 gatgaaataa aagctcaaca tgaccatatt ttggctcaag aacggtggat tattgatggt
    11581 tggggaaatt gggatctaat cgaaacgcgc tttgcggcct cagatacgat tatttttgtt
    11641 gaccatccgc tcttggttca ttattggtgg gccctaaaaa ggcaatttgc gtgcatattt
    11701 gcaccgagag tagatgggcc tgaagattgt ccaatgctgc ctatgacact gcctttgcta
    11761 aaaatgatct ggaacattca ttatcattcg cgtcctcggc ttcttgaatt ggtaaatcgt
    11821 tttcgtgcag ataagcaagt aattcatatc acgtcgccta aagaattacg tttgtttatg
    11881 gcgaaatact gagtttgttg gcaaactcat ctgcttgcat catcgccaaa cgcagatcgg
    11941 agccgggtac gtaaagacca tgccactgaa aacctggcgg tcatccgcca cttgcgctta
    12001 atctgcattg ccctgaaacc caaccgccca acggttgcca tctggccccg tctgggctac
    12061 aatctgactc atggattggc agattgccgg acacgactgg gccgcccgcc tgctgcagca
    12121 gcagttggcg cgcggcgaaa cgcgccacgc ttacctgttc accgggccgc cgggggtggg
    12181 gcggcgcacg ctggcgctgc ggctggcaca ggcgctcaac tgcctgcagc cccccgagcc
    12241 cggacagttc tgcggcaaat gccgggcctg ccagcagact gccgccatgc agtttcccga
    12301 tctgacggtg gtgcagtcgg cccaggtggg cgatgaactg aagattgacg ccatccgggc
    12361 gctgtcgcac acgctctcgc tggcgcccta tgcggcgcgc tggcgcgtgg cgctgctgct
    12421 gcgctttcac gaagccaacg ccaatgccca gaacgccctg ctcaaaacgc tggaagaggc
    12481 gccccccagc gccatcctgc tgctgaccgc tgactcggca gaagacctgc tgcccaccat
    12541 ctccagccgc tgcgaggtgg tgcggctgcg ccccctggga ttggacgccc tgaccgccta
    12601 cctgacgcag aatcacagcc tgggcgcggc cgaagcccgc caactggcgc agatctctgc
    12661 cgggcgggtg ggactggccg agcagtacct ggccgatccg caggcagttc aggcgcgggt
    12721 tgagcagctg gacgagctgt ggcagatgct ctgtgcgccg caccgcgaac ggtttttata
    12781 cgccaagaag atcgccccgc catcatccaa acaggagcgc agcctcaccc gggcggcgct
    12841 gcgcggccgc tttgagttgt ggctctcctt ctggcgagat gtgctgctgc aggtcaacgg
    12901 cgcggccgcg cccctgagca accccgaccg ggcggcgcag attgctgcgg tggtggcggt
    12961 ggtgacgccc actcaggcgc tgcggcaggt caccgcgctg gagcaggcca ttgaccgcct
    13021 gccgggcgcc aacctgcagc tcctggtaga ggtgctgctg ctcgactggc cgcgcctgcc
    13081 gggcggcagc gcacttgcca gcctgccgga gatggtcgca gaggattaac cccgggggga
    13141 gattcagccg cggctttcgg ccagcacgga ttctacggta gcttccaggt ccagcgtttg
    13201 accgcgggcc agggcggctt gcagtccggc agcgggcagg gccgcctgca ggtggggcag
    13261 cacaaagtca gccgcctgag tcacatcgct attggtggcc gggtgattaa gcgccagccc
    13321 cagcagttcg gcggcgcgct cgggttggcc gccagcccag tagcagcccg ccagcccggc
    13381 cagggcctcc agcaagcggg ggggcgcgcc aatttcctgc gcttcgacaa ttgaacggcg
    13441 gtaatagccg ctggcaaccg cccaatcacc ctctggccag gcggcgtggc ccaggttggt
    13501 caggcaagag atcacgctct cgcggtcgcc cagttcctcg gccagcgcca ggctctcttc
    13561 tgaatagcgc cgcgaggcgg cataatcttg atgcagcagc gaggcccaac ccaggctaat
    13621 caaaattcgc accgagagca tggggtcgtc cacctggcgg gccagcgcca gcgccgattc
    13681 aaaatattgc agggcggtgt tcaggtcatt cagagtgagc gccacagacc ccaggctgtt
    13741 aagtgagctg cactcgccgc gccggtcgcc aatttcacgc gcaatttgca gtgattcggt
    13801 gtgcgcttgc cgggcggata ccacatcagc caggtcatgg taaaccatgc ccagattgcc
    13861 caacgcagcc gaaatgccag aacggtcgcc aatctcgcgg tgaataacca gactctgctc
    13921 aaaataacgg attgccgcgg ggtaatcacc ctgtgagaac gccaccagcc ccaggttgct
    13981 caggcaggtg gccataccat tgcgattgct gttttggctc tctaaattta atgaagccgt
    14041 gatgtgctca atggcagaaa tatattcagc ctgataatag gcaatcacgc ccagcacacg
    14101 ttcggcctgc gccgccagca gtgggtcctg ggtctgagcc agcaccgcct gattcagtcg
    14161 cgtggcctca tcataatcgc cctgacgcat caacactcgc cccagcccca gggtggcctc
    14221 caccatcagg tcgtgggctt gcaggtgctg gctgtcagcc agggcctgct gcaaatgctc
    14281 agcagccggg tcataatcac cggtgcgttc cagggcgttg cccagccgga tttgcagcgc
    14341 ggcgcgctcg ttgaggtggt ctgggggcag cagcgtcagc gcgtgttcaa atgcggcgat
    14401 gctttcgggc gcggcaccgg tgcgcagagc atcgtccccc agacggcgca gccaggccgc
    14461 agcccgggcg ggttcgccag ccagttcata gtgccgggca atgacggcgg caaattctgg
    14521 caggcgctcg ccagccgaat cttccatcca ctcggccacc tggcggtgat aaattcgccg
    14581 cagtttgagc agcacggttt cgtaggcggc ctcgcgcagc atactgtgct tgaaggtatt
    14641 ttcctgggca ccggcaaagg tggagcgcat ctgctgaaag atcagctcgc gccattgcag
    14701 ttttgccagc agcgacgccg tatcggagac ggtatcgccg ggggcgtgca tggccttgag
    14761 cgtggcatcc cagaaggttt gccccaccac ggccgcgtgc tgcagcaggt cgcgttcgtc
    14821 tcggggcagg ccgtccaggc gggcctgcag cagttcggtc agcgtggcgg gcacatgggc
    14881 gcgctgcagc tgctcaacct gcacctgcca gtgatcttct ccccgcagaa tgacaccatt
    14941 ttcgatgaag gttttgatca gttcttccac atagaagggg ttgccttcgg cgttttgcac
    15001 aatcaattcg cgcagcggct cgggcaggtt ttgcaccttg tgcaagatct cgccgaccag
    15061 gcggcggctc atctccggcg agagcggctg caggtcaatg cgcaaggcag ccgggccgcg
    15121 gctccagtct tcccgccgct caaacaggat gggccgcgtc aggcagatga tcatcaggcg
    15181 ctggtcagac aggctggcgg tcaggttttc cagcaaatcc agcgaggagg catcggccca
    15241 gtgaatatcc tccataaaca cagccagcgc cccggagctg gccagggcgc gaaaataggt
    15301 caccaggtag gcctgggcca gccgggcaaa ggatgggctg cccagcaggt tctgcactgc
    15361 cgccgtgccg gaaaaatcga agcccaccaa ctgccccacc agcgcaatct gatcgggcgg
    15421 caggctgccc gccatgccag cctcaaactt ggcgcgcacg gtggccgggc ggtcgctttc
    15481 cagaatatca aaccggttgc tgaacaggct gcgcagaatg ccgtagggga tggactgcat
    15541 ttccggggtg gcgcgcccct gaaagagata gacccgctcg ggcagggcgt ctagccagga
    15601 ttcaaactcg atcagcaggc gcgatttgcc aatgcctgcc tcgcccacaa tgctgaccag
    15661 ccgggcctgc gcctggtgca tggcagattc aaaggcctgc tgcagggcgg ccagttcggc
    15721 ctcgcggcca atcatggggg tctgaacccc ctccacgccg cgggtggcca tacggaagga
    15781 tcgcggccgg gcgcgctgta caatgtaggt ctgcaccggc tcgcgcttgc ctttgagcgt
    15841 gatcggctcc tggggctgca catcaaagat gccgcgcacc tgccggtaag catcgtgaga
    15901 gatgatcacc ccgccaaccg gggcggcatt ttgcagccgg ctggtcaggt tgacggcatc
    15961 gccggtggcg gtgaattcgc tggttgtgcc caccaggccc agcagcaccg ggccggtatg
    16021 cacccccacg cgcatccgca cggggtgggg ccagtcggtg gtcaggctgt tcaactcggt
    16081 ttgcatttcg agggcggtgc gcacgcactg ttcggcgtca tcctcgcggg cggtatgcac
    16141 gccccagatt gccatcacgg catcgcccat gtgtttgtca atatagccgc cgcgctgcag
    16201 aatcagggca tccagccgtt cccagagggc gttcatcagg tcgtgcacct cttcggcatc
    16261 cagcgtttca gagagggccg taaaacccga gacatcggca aatagaatag tgacctgttt
    16321 gcgttgggcg gcaggggccg cctgggcctg cagttcagcc agtttctggc gcaggccagt
    16381 cagggcagca tccacagcgg tgtcacccag cagggcgcgc tgggcttcga gagcagccat
    16441 ggcctgttga atcgtctcta tgtcgctcat attttctcca gccgggggtt gaattgagca
    16501 tacctgaaag cccggagaat gtcaaacagg aattaggaac taggttttag gttttaggtt
    16561 ttgggttttg gggattgagg attgggttcc acgttccagg ttccaggtgc gatggtgggg
    16621 cccatcggat tcgtaggggc ggggcgagac actccgggcc acaattgaca tagaaccggc
    16681 ctcgcgccat gttacagcta aacgctaatg ggccaggccc ccttccctca ccgctccgga
    16741 gggagcgggg cttaagcccc cttcccttta gggcttatcc atacccacaa ataatggata
    16801 gtctccctaa ttagtgtgtt tagtatctgg tacaggcacc aaatgcaata g
//
LOCUS       DAOPVS010000030        16505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3566_length_16505_cov_53.683830, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000030 DAOPVS010000000
VERSION     DAOPVS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16505
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3566_length_16505_cov_53.683830"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="PKL11_04455"
     CDS             complement(<1..2004)
                     /locus_tag="PKL11_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ12886.1"
                     /translation="MQKGWRTLGIILIVMLTVGVTPKATQAEQIESLGVTAIHRSGQT
                     FITWTESAGLTNELYTIYRSNEPITAANIHRAQVLLTVPEDSARFYANRYYKDYVGPW
                     LNRYLDRLVIADGQPQLAAGTGLLVWTLNEFDFQDYPEMAGYYAVTYTPYGGSEIFDP
                     ANVTGRVSESLLPPKPVRTAVNLGGNAYLMIQYMDLHSWNPTFHAPNATNNYYGNNPN
                     DFKIQHAVQYAYDYAISTPTAAQCDGSVPPVVPVVMHLHSWGDNTYDPYLPGQAPDWC
                     ATIIWPIDMGETWFFGFAQENDYRISEMPGPGDVIQNYTEQRLLRMIYDQLHNPVGPE
                     IDPERIYVKGASMGANGALALALRYPDVFAAAYSSQPMTNLRASTFDDAFFVADAAIS
                     WGWPEDELPVYLTAPNGWADNLQSYNGESVWEWQNHLQTISERKQVDAAPLSLAWGIE
                     DIVMDWATQGRPSFEVLNFSQQCWGASISNDGHHDQSYRGLMPNYIYLPGSGPFWGLQ
                     ARNNETVPGLSDFSSNPALPPPNNPPPVPGEYISFNATIRWAATWDAWDAPPVDEPDR
                     WEMSLCAISSESATCGTGSVQTVDVTPRRVQQFEVRPSQFYRWENWRLSDGGLYAEGF
                     TTMNASGHITIPGAVIFPQGSRLVLFPVDTMRVFLPAILCGTNN"
     gene            2090..3220
                     /locus_tag="PKL11_04460"
     CDS             2090..3220
                     /locus_tag="PKL11_04460"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNZ12887.1"
                     /translation="MDIFTRIEKPTLLLDVERARANIRQMAQKASAQKVRFRPHFKTH
                     QSATIGEWFAAEGVKAITVSSVDMARYFISYGWKDVTIAFPVNLRQISVIESLAQRAS
                     LGLLVESVEVARVLGEKIRSDVSIWLKVDVGSHRTGLAYDAPQPFLSIISELSRHSHL
                     KLRGLLAHAGHSYRAINAAQVCQTYSESVERLLALRSALLAAGSPPLEISVGDTPGCT
                     LSPQLGPVDEIRPGNFVFYDVQQLGVGACTPDQIAVALACPIVALHPEREIAVIYGGA
                     IHLSKDTANWDGDHIYGLVALPNPTRQPGQPGWGEPLPGAYVASLSQEHGLVHVPKPL
                     MEQLHIGDLLCVLPVHSCLTAQAIGRYLTLEGERILMMPLMG"
     gene            complement(3228..4481)
                     /locus_tag="PKL11_04465"
     CDS             complement(3228..4481)
                     /locus_tag="PKL11_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNZ12888.1"
                     /translation="MDIPVEISGYDSNVRQIMRVEDVTLGTAQQGFIARYRGQIINPD
                     TEAAYEQLSNALKPHNLTPLFRWDEKRHAILIIEGLPKPTPDNTRTNLIMFLLTLVSL
                     LVTGGFLAFGATIPKGFGAIVLGIITQGWPFALSMIAILGSHEMGHYLMGRHHKVHVT
                     LPYFIPLPYPISPFGTMGAFINMKEIPRNRRQLLDIGAAGPLAGLIVAIPVILIGLAL
                     SPVTPLPSAEQICPPGTMVEMFAPLPTDCEPASSLEGNSILYLLLKYISKGEMLPAPA
                     AYTEAPALHWLKYIFTGHPLPFGGRDVTLHPVAWAGWAGLLVTAMNLIPAGQLDGGHV
                     LFVLLGGKRTRKTVPIILILLGLLGFVWPGWWLWAVLIFFLGRAHAEPLDQITPLDGK
                     RKALAVLMVVVFLLVFVPVPLMIMQ"
     gene            complement(4548..4641)
                     /locus_tag="PKL11_04470"
     tRNA            complement(4548..4641)
                     /locus_tag="PKL11_04470"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4605..4607),aa:Sec,seq:tca)
     gene            complement(4697..5656)
                     /locus_tag="PKL11_04475"
     CDS             complement(4697..5656)
                     /locus_tag="PKL11_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ12889.1"
                     /translation="MHYRRMGKTGLKVSEISLGAWVTFGNQIDQKTASDLIHAAHEQG
                     INYFDNADVYAGGQAEVVMGQAIKGLARAELVISSKVFGPTMPGPNGRGLSRKHIMES
                     IHASLKRLDTDYLDLYFCHRYDPDTPMEEVARAMDDLIHQGKVLYWGTSEWEAAQVAQ
                     AIGVARQYGLVPPVVEQPQYNLFHRNRVEMELAPLCKEYGLGLVTYSPLYQGLLTGKY
                     NDGIPSGSRADRDDMAWVRDRITPEKVEVVRHLTVIANDLGITPAQLAIAWILRRKEV
                     SSVITGATRIEQLDDNLTAAEAVDNLSDEILEHIEQIVGNQPD"
     gene            complement(5720..7057)
                     /gene="ablA"
                     /locus_tag="PKL11_04480"
     CDS             complement(5720..7057)
                     /gene="ablA"
                     /locus_tag="PKL11_04480"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNZ12890.1"
                     /translation="MSPTPFTSKRAPYYANVPDEKWNDWRWQMSNRLNSLEDFEHVLN
                     LTDSERKALSTSGLFRVDITPYYASLIDPDDPNDPIRLQVIPRDNEILPFTGMMEDSL
                     SEDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRSRIVGDPSATFSRAEFEAQIEY
                     LKNTPQVRDVLLSGGDPLALAPKILEELLSRLREIPHIEIIRIGSRIPVFMPQRVTPE
                     LCDTLQKYHPLWMNIHVNHPNEITAELAEATDRLTRAGIPLGNQSVLLAGVNDCVHIQ
                     RRLVQDLVRIRVRPYYLYQCDLVEGAGHFRTPVAKGVEIIEGLRGHTSGYAVPTYVVD
                     APGGGGKIPVMPNYMISMSDHKIILRNYEGYITTYEEPTDYKAHDPKTCPHCQSKRVE
                     PGQGGVMGLLDGEDMFIKPEGFDELHNRGGGSHRLRDNKDKWKPLGIGSGEEA"
     gene            7388..8461
                     /gene="hrcA"
                     /locus_tag="PKL11_04485"
     CDS             7388..8461
                     /gene="hrcA"
                     /locus_tag="PKL11_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNZ12891.1"
                     /translation="MIAEQLTERQKFILTLVVHDFTRTATPVASQHLVERYHLDMSSA
                     TVRNEMVVLTEKGYLRQPHTSAGRIPTEEGYRYFVGHLLRATELPDDERRTISHQFYQ
                     TRQDMGQWVRLAASVLAHQSRAASLVTAPHPAEVRFRHLELISTRGLQVLIVLVLVGG
                     EVRQRLVSLDLVASQEQLSALADHLTNLFSGKDALGMRTLATQLTGMEQQFAYWVIED
                     MSDIQSAPTGEVFLDGVSNVLSEPEFIGSEDARRALQLLEERSLLQDLLARSVLTDRT
                     GGVQVLIGGEGTREELRPFSMVLASYGAPGLAMGTLGVLGPMRMSYGRSISTVRFLSS
                     LLSNLVAETLTDDNKEGLEEEFD"
     gene            8461..9087
                     /locus_tag="PKL11_04490"
     CDS             8461..9087
                     /locus_tag="PKL11_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNZ12892.1"
                     /translation="MVEDKRRKHKPEAPPPADTPEVSPEAGADEEIISAVRPQPAEES
                     EMILVSKKELAEMQAQLESARQEAGENMNGWQRERADFLNYKKRIERDQVLQTQSIKA
                     DLVKRYLDVLDDLERALQSENRPHAGKSAQWGSGIELIYRKLQGILEAEGVQRIPAEN
                     EIFDPNRHEAITSEDSSTHESGQVIGVVRQGYMIGDRVIRPALVRVAK"
     gene            9102..11003
                     /gene="dnaK"
                     /locus_tag="PKL11_04495"
     CDS             9102..11003
                     /gene="dnaK"
                     /locus_tag="PKL11_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNZ12893.1"
                     /translation="MAKIVGIDLGTTNSVVAVMMGGEPVVIPTVEGERLLPSVVAVNK
                     NHERLVGRSARNQAVMNPENTVFSIKRFMGRKFDDPEVQRTISRVPYKVKAAANGDVR
                     VVLDGKEYSPPEVSAMILAKLKADAETYLNEPVTQAVITVPAYFNDAQRNATKDAGRI
                     AGLEVLRIINEPTASSLAYGLDKKKNEVIAVYDLGGGTFDISILDVGEGVFQVRSTSG
                     DTFLGGDDFDIRVMDYFIDEFNKQSGMDIRQDRQALQRLREAAEKAKIELSTVMQTEI
                     NLPYLTADASGPKHLVMTLTRAKLEQLTEDLVKKSLDPVRRALADAGLKASDINEVVM
                     VGGMTRMPLVQEMVRREFGKELHRGVNPDEVVAVGAAIQAAVLGGEVKDILLLDVTPL
                     TLSIETLGGVATPIIERNTTVPTRKSQVFSTASDGQTTVEIHVVQGERPMAADNKSLG
                     KFQLDGIPPAPRGIPQVEVTFDIDANGTVKVTATDKATGRAQHITIAGSSGLNEQEIE
                     RMRREAEAHADEDRRRKEMVEVRNNADNMIYTTERTLREAGDKVPADQRKQAEDAVAN
                     LRSLMEGGDPEAVRKATDQLGELIQKIGASMYQQQPPTGGPTPDSGAPGNGQTPGGDD
                     VVDGEFRNL"
     gene            11067..12182
                     /gene="dnaJ"
                     /locus_tag="PKL11_04500"
     CDS             11067..12182
                     /gene="dnaJ"
                     /locus_tag="PKL11_04500"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNZ12894.1"
                     /translation="MTSRDYYEVLGVPRDASPEDIKSAFRSLARQYHPDVNREAGAEE
                     KFKEINEAYGVLSDTEKRSTYDRYGRSGLGDMGGMPDFTMSDFADLFGEFFGFGGMGR
                     QQRRNMPQRGADLLYTLTLTFEESVFGVEKEIQVTRDESCSNCRGSGSEPGSGPTRCT
                     TCGGRGEVRQVRQTILGSMVTVAPCQACNGRGEVITNPCHVCKGRGLEQRTTRKLVTI
                     PAGVDNGTRLRLAGEGQPGANGGPNGGLYIDISVKQHAYFRRRGDDILLNLNINIAQA
                     ALGADVEVPTVDGPVRLSIPAGTQPGKTFTIKGKGVPRVRSSSRGDQIVVANVEVPSR
                     LTPEQRQLFEQLSQTLGTEVKPRERGFLDLLKEVFGG"
     gene            12175..13092
                     /gene="prmA"
                     /locus_tag="PKL11_04505"
     CDS             12175..13092
                     /gene="prmA"
                     /locus_tag="PKL11_04505"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNZ12895.1"
                     /translation="MAEARWLEISLVVNDELAEAVADVLGRFVSQGVVMERSVEFNDA
                     EDEGTPYGPVRVSGYLPVDEQLEDVRQQLEQALWYLGRIQPLPEPSYRTIEDQDWMAA
                     WKQHYRPIPVGQKLMILPAWLSLDTEDRIAVRIDPSMAFGTGTHPTTQLCMALLENRV
                     SENEPVIDVGCGSGILSIAAVKLGAVRALAVDIDPLSVKATLENAQANGVSEKIEAGL
                     GSVAEILQKNFSLDRAPLVLANILAPVIVRLLDAGLAQLVLPGGILILSGIMVDQADS
                     VIEAAARHQLTLIEQQTMGDWIALVMQAK"
     gene            complement(13202..14431)
                     /locus_tag="PKL11_04510"
     CDS             complement(13202..14431)
                     /locus_tag="PKL11_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNZ12896.1"
                     /translation="MITLSIQQARRFLLRHHFLYPARQLTGTDGVMQIFERLGSIQFD
                     PVEPSFGRNAELVLQSRVAGYQPVLLEGLLYQERRLWDGFDKVMCIYPTQDWPNFARR
                     RALMRVHYGGEEHRPMQLAEMVRSELQARGPLSSIDFDHDGRADWFWGETRLVRATLE
                     TLFSIGELGIHHRVNTRRIFDLSKNLLPEAVLNALDPFASDEAYQDWHVLRRIGGLGL
                     AQVGSGEHWLGIVGTKSPQRQAALKRLTEAGLVTPVKVTEIPRQTFYARTADLVHLDE
                     MNSCNPTEAAFLAPLDNLLWDRKLIQWIFGFDYIWEVYKPATIRQYGYYVLPVLCGER
                     FVARFEPKFDKKSRRLTVQNWWWEADVTLDQRMQAALARALKDFGTYLNADEIALGAA
                     IENDHSLIGVIGAAKSL"
     gene            14587..>16505
                     /locus_tag="PKL11_04515"
     CDS             14587..>16505
                     /locus_tag="PKL11_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12897.1"
                     /translation="MNNQFCVGQRSVARILIALIVVVLLAGLIQPVAADSQMPSEPAP
                     QIQAVNNSHWSTEFYPRGVNGYVSDLEIGPDGSLYIAGRFYQAGTAQVTGLARWKNNQ
                     WQAFGNFTSAYYSGPYTGLGYVNDIAISGQNIYVGGFFRYYNHYVSANNVAVWDGTRW
                     SRLGYGIDDEVTALTLDSSGILYAGTYSGDIYSWDGTSWTIQGSADDTINALAFDDAG
                     TLYAAGFFTTLDGVSVNYIAMKSGGTWQPMAGGASRNDGKDPWVSDIVFWNGTLYAGG
                     WFDAMGGIPASHLAYWQAGTWHAITGLDAGVYDLQPDGNKLWVGGTLDTISGLQTGNL
                     AAWNGISWEVLPEQISNQSVSAIAVNGTHLFAGGNIFEAGQTGFSSIAEWTGSAWTAL
                     GEGQGAADEIQSVLLPPDQNSLYVGGRFDSIGGTPAHSIARWDGSQWHTLGDGFKRGT
                     LVADVHALLMAGDVLYAGGYFSSSGSTPLSNIAQWNGNAWLPLGEGLASTETSGRVYA
                     LTADDAGNIYAGGYFDKSGEIIVNRVARWDGASWLPLGDGLDYTVRALAYDTTNDLLY
                     AAGTPNGIPEPSFIGVSVWDGSSWQPLGTEPTGFVFAMKISPDGILYAGINGYELAYW
                     TDNAWHRISEQAISSS"
BASE COUNT         3640 a         4762 c         4317 g         3786 t
ORIGIN      
        1 attgtttgta ccacaaagta tcgccgggag gaagaccctc atcgtgtcaa ccggaaagag
       61 aaccaaccgg cttccctgtg gaaaaatcac cgctcctgga atggtaatat gcccgcttgc
      121 attcatcgtg gtaaaaccct cggcataaag cccgccgtcg ctcaggcgcc agttctccca
      181 ccgatagaat tgggatggcc tgacctcaaa ctgctgcacc cgtcgtggtg taacatcaac
      241 cgtctgcaca ctgccggttc cgcaggtggc tgattcagaa ctgatggcgc acaggctcat
      301 ctcccagcgg tccggctcgt caactggcgg cgcatcccac gcatcccatg tggcggccca
      361 gcgaatggtg gcgttaaagc tgatgtattc ccccggaacc gggggcggat tattgggcgg
      421 cggcaaagcc ggattgctgc taaaatcact caaccccgga acggtttcgt tattccgagc
      481 ctgcagcccc caaaatggcc cgctgcccgg cagatagata taattaggca tcaatccccg
      541 gtagctttgg tcgtgatgtc cgtcattgga tatggatgcc ccccagcact gctggctgaa
      601 gttcagcacc tcaaacgatg gcctcccctg cgttgcccaa tccatcacaa tatcctcaat
      661 cccccaagcc agactgagcg gtgcggcatc cacttgcttt cgttcgctga tggtttgcag
      721 gtggttttgc cactcccaaa cgctttcccc gttgtaactt tgcagattat cggcccaccc
      781 gttgggcgca gtcagatata ccggcagctc atcttcgggc catccccaac tgatggccgc
      841 gtcggccaca aaaaaagcat cgtcaaacgt actggcgcgc agattagtca tcggctggct
      901 ggaataagcg gccgcaaaaa catccggata gcgcagtgcc agtgccagtg ccccattggc
      961 gcccatcgag gcccctttga catagatccg ctctgggtca atctccgggc ctaccgggtt
     1021 atgcaattgg tcatagatca tccgcagcag ccgctgctcg gtgtaatttt gaatcacatc
     1081 acccggtccg ggcatttcag agatccggta atcgttctcc tgtgcaaatc caaaaaacca
     1141 ggtttccccc atatcaatcg gccagattat ggtcgcacac cagtcgggcg cctgccccgg
     1201 caggtatgga tcatacgtat tatcccccca gctgtgcaag tgcatcacca ccggcacgac
     1261 tggaggcacg ctgccgtcgc attgcgcagc cgttggcgta ctgattgcgt aatcgtaagc
     1321 atactgcacc gcatgttgaa ttttgaagtc attcggatta ttgccatagt aattgttggt
     1381 cgcattgggc gcatgaaacg ttggattcca actgtgcaaa tccatgtatt ggatcattaa
     1441 ataagcattc ccacccagat tgacagccgt ccgtaccggc ttgggcggca gcaggctttc
     1501 tgaaacccgg ccggtcacat ttgccgggtc aaaaatctca ctacctccat agggcgtata
     1561 ggtcacggca tagtacccgg ccatttcggg atagtcttga aaatcaaatt cattgagcgt
     1621 ccagaccagc agaccggttc ctgccgcaag ctgcggttgc ccgtctgcaa tcaccagtcg
     1681 gtccaggtat cggtttagcc agggtcccac ataatctttg taatatcgat tggcataaaa
     1741 ccgtgccgaa tcttcgggca ccgtcagcag aacctgtgcc cggtgaatat tggccgctgt
     1801 gatgggttca ttggaacggt aaatggtgta caactcattg gtcagccccg ccgattcggt
     1861 ccaggtaata aaagtctgtc cgctgcgatg aatcgctgtt acgcccagcg attctatctg
     1921 ttcagcctgt gttgcttttg gcgtaacccc caccgtcagc atcacaatta aaatgattcc
     1981 cagcgtccgc cagccttttt gcataatgcc atctaccgat ttccagggat gatgtttgac
     2041 actcccctaa ttatagtgtc tctcgttata atccatctgg agaaaaaaca tggatatttt
     2101 tacccgcatc gaaaaaccca ccctcttgct cgatgttgag cgtgcccgtg ccaatattcg
     2161 ccagatggcc caaaaagcct cggcgcaaaa agtgcggttt cgcccccatt tcaaaaccca
     2221 ccagtctgcc acaattggcg aatggttcgc ggctgaggga gtcaaagcga tcaccgtctc
     2281 atccgtggat atggcgcgct actttatctc ctatggctgg aaagacgtga ccattgcctt
     2341 cccggtcaat ctgcgccaaa tctcagtcat cgaatcactg gctcagcgag ccagcctggg
     2401 tctgttggtg gaatccgtag aggtggcgcg ggtactgggc gaaaaaatcc gctctgatgt
     2461 gtccatctgg ctcaaggtgg atgtcggttc acatcgcaca ggcctcgcct acgatgcgcc
     2521 tcaaccattc ctgtccatta tctccgaact atcccgtcac tcacatctga agctgcgcgg
     2581 cctgttggcc cacgccgggc attcctaccg cgccatcaat gctgcccagg tctgccagac
     2641 gtattccgaa agcgtggagc gcctgttagc cctgcgatct gctttactgg ctgctggttc
     2701 ccccccattg gagatttccg tgggcgatac ccccggctgc accttatccc cacagcttgg
     2761 tccggtggat gaaatccgcc ccggcaattt tgtcttttat gatgttcagc agctcggcgt
     2821 gggcgcctgc acccccgacc agattgcggt tgccctggcc tgccccatcg tcgcccttca
     2881 ccctgagcgc gaaattgcag tgatctacgg cggcgccatt cacctctcta aagatactgc
     2941 aaactgggac ggtgaccata tttatgggct ggtcgctctg cccaatccca cccgccaacc
     3001 cggccaaccg ggctggggcg agcctttacc cggcgcctac gtcgcctccc tgtcacagga
     3061 gcatggtctg gtacatgttc ccaagccact aatggaacag cttcacattg gcgatttgct
     3121 ctgtgtcctg ccagtgcatt cctgcctcac cgcccaggcc atcgggcgtt acctcacctt
     3181 agagggtgag cgcattctga tgatgcccct gatgggctaa agcggcctta ttgcataatc
     3241 attaacggca caggcacaaa caccagcaga aaaaccacaa ccattaaaac cgccagtgct
     3301 ttccgtttgc catctagcgg cgtgatctgg tccagcggct cagcgtgagc gcgtcctaaa
     3361 aagaaaatca gcacggccca caaccaccat ccaggccaca caaatcccag cagccccagc
     3421 agaatcagga tgatcggtac tgttttacgc gtccgtttgc cgcccagcag cacaaacaaa
     3481 acgtgtccgc catccaactg tccggctgga atgaggttca ttgccgtaac cagcagcccg
     3541 gcccagcctg cccaggcaac cgggtgcagc gtcacatccc gcccgccaaa tggcagtgga
     3601 tgccccgtga aaatatattt gagccagtgc agcgccgggg cttctgtgta agcggcgggc
     3661 gccggcagca tttcaccctt gctgatatat ttcagcagca aatacaaaat tgagtttccc
     3721 tcgagtgacg aagccggttc gcagtccgtt ggcagcggcg caaacatctc gaccattgtt
     3781 ccaggcgggc atatttgctc ggcgctgggc agcggcgtta ccggcgagag cgccagccca
     3841 atcaaaatga ccggaatggc aacaatcagc cctgccagcg gcccggccgc gccaatatct
     3901 agcagttggc ggcggttgcg gggaatttct ttcatgttaa taaaggcccc catggtcccg
     3961 aatgggctga tggggtaagg cagggggata aaatagggga gggtcacatg gactttgtgg
     4021 tgacgcccca tcagataatg ccccatctcg tgagacccca ggatggcaat catgctcaac
     4081 gcaaacggcc atccctgcgt gataattccc aggacaatgg ccccaaaccc cttggggatt
     4141 gtcgccccaa acgccaggaa cccacccgtg accagcagac tgacgagcgt cagcaggaac
     4201 atgatcaaat tggtacgggt attatcgggg gttggtttgg gcagaccctc aataatcaaa
     4261 atggcatggc gtttttcatc ccatcgaaac agcggtgtca gattatgcgg tttgagcgcg
     4321 tttgaaagct gctcgtatgc ggcttctgta tccggattga tgatctggcc gcggtagcgg
     4381 gcaataaaac cttgctgtgc ggtgcccagg gtgacatcct ccacccgcat aatctgccgc
     4441 acattgctgt cataaccgct gatttcaacc ggtatatcca taaatccatt ccaatctcta
     4501 cagatacttt cggctgacgg cgtagtccac cgtcagccgt ttctaggcgg gaatggatgg
     4561 gagtcgaacc caccacggcc cgcaacgcga cccgtcaccg gttttgaaga ccggaaagcc
     4621 caccgggact catccactcc caaacttaag catacttcag ctgcttgcct tttgcaagga
     4681 aaattccatc taaaccttaa tctggctggt tgccaacaat ctgctcaata tgctcgagaa
     4741 tctcgtcact taaattgtcc acagcctctg ccgctgtcag attatcatct agttgttcaa
     4801 ttcgggttgc tccggtgatc acactgctga cctctttgcg ccgcagtatc caggcgatgg
     4861 caagctgtgc cggggtaatt cccaggtcat ttgcaatcac ggtcaggtgc cgcaccacct
     4921 caactttttc aggcgtaatt cggtcccgaa cccaggccat atcgtcgcga tctgcccggc
     4981 tgccgctcgg gattccatcg ttatatttac ccgtcagcaa gccttgatac agcgggctgt
     5041 atgtcaccaa ccccaaacca tattccttgc acagcggcgc cagctccatc tcaacccgat
     5101 ttcgatgaaa gagattgtac tgcggctgct caaccactgg cggaaccaat ccatattgtc
     5161 gcgccacgcc aatggcctgg gccacctggg cagcctccca ttctgatgtt ccccagtaca
     5221 gcactttgcc ctggtggatc aggtcatcca tagcccgcgc cacttcctcc atgggggtat
     5281 ctgggtcgta gcggtggcag aagtatagat ccaggtaatc cgtgtcaagc cgcttcagcg
     5341 aggcatggat ggattccata atgtgctttc gtgagagccc gcgcccgttg ggtcccggca
     5401 tagtgggccc aaacactttg ctggaaatca ccagttcggc gcgggccagt cctttgatgg
     5461 cttgtcccat cacaacctca gcctgcccgc ccgcataaac atcagcatta tcgaaatagt
     5521 taatcccctg ttcatgtgct gcatgaatga gatcactggc agtcttttgg tcaatctgat
     5581 tgccaaaagt aacccacgcc cccagagaga tttcactcac cttgagccct gttttgccca
     5641 tccgtcggta atgcatccta cctccttcgt ttgagaaaga gggcggcagc cggtataagg
     5701 gctgccgccc tcgcacagct tacgcctctt cgccagagcc aattcccagc ggtttccact
     5761 tatctttatt atcgcgcagt cgatgcgacc cgccgcctcg attgtgtagt tcgtcaaaac
     5821 cttctggctt aatgaacata tcttcgccat ccagcagccc catcacgccg ccctgacccg
     5881 gctctacccg cttgctctgg cagtgcgggc aggtcttggg atcgtgcgcc ttataatccg
     5941 taggctcttc gtaagttgtg atgtagcctt catagtttct caagataatc ttgtggtccg
     6001 acatgctgat catgtaattc ggcattaccg ggattttacc gccgccgccg ggagcatcca
     6061 ccacataggt cggaaccgcg tagccagacg tgtgtccacg cagcccttcg atgatctcta
     6121 cgcccttggc aaccggtgtg cggaagtgtc cggcaccttc caccaggtcg cactgataca
     6181 ggtaatacgg gcgcacccga atccgcacca ggtcttgcac cagccgtcgc tgaatatgca
     6241 cacagtcgtt cactcccgcc agcagcacac tctgattgcc cagcggaatg cccgcccggg
     6301 tcaggcggtc agttgcttct gccagctcag cggtgatttc gttggggtgg ttgacatgga
     6361 tattcatcca caggggatga tatttttgca gggtgtcgca taattccggc gttacccgct
     6421 gcggcataaa caccggaatg cggctgccaa tccggatgat ctcaatgtgc ggaatttcgc
     6481 gcagtcggct taacaactcc tcaagaattt tcggcgccag cgccagcgga tcgccgcccg
     6541 agagcagcac atcgcgcacc tgcggcgtat tcttcaaata ctcaatctgg gcctcaaatt
     6601 ccgcccgcga gaaggttgcg cttgggtcgc ccacaatgcg agagcgagtg cagtaccggc
     6661 agtaagaggc gcattgggtc gtcaccagca tcaacactcg gtccgggtag cggtgcacca
     6721 gcccgggaac cggcgagtgg cgatcttcgg acaacgaatc ttccatcatc cccgtaaacg
     6781 gcaaaatctc gttgtcgcgg gggataacct gcagccggat ggggtcattg ggatcgtctg
     6841 ggtcaattag agaagcgtaa tagggcgtaa tatccacccg gaacagaccg ctggtgctca
     6901 gcgctttgcg ctcgctgtca gtcagattca acacatgctc aaaatcttcc agcgaattga
     6961 gccggttgct catttgccag cgccaatcgt tccacttctc gtcaggcacg ttggcataat
     7021 acggtgcacg tttagatgta aatggggttg gagacatagt caatcctttt ttgaaacatt
     7081 aaccgaataa tacgtcagcg catcgcgcca aatacgtcag agcagcaaac cagcggcgga
     7141 tcatggtcag tgcggtagaa acctaaaaac agattgctgt cgaacgataa aatcatgtgt
     7201 ccattttaaa gaataaacct cgtctggtca atcattttta agattactca taattattcg
     7261 gtcctgctgt cctcatacta ttaacaattt gtcagaactt cccccacggc attgactttg
     7321 cccttcaggc atggtattta atacgcaggt tgccgcgttt agcagactcg gctcaagagt
     7381 gctaaaaatg attgcagaac aactgaccga acgccaaaaa tttatcctta cgctggtcgt
     7441 gcatgatttc acccgcacgg ctacgccggt cgcgtcccag catctggtgg aacgttacca
     7501 tctggatatg agttcagcca cagttcgcaa tgaaatggtt gtactgaccg aaaagggtta
     7561 cctgcgccag ccgcacacct ccgccgggcg cattcccacc gaagaaggtt accgttactt
     7621 tgtcggccat ctgctccgcg ccaccgaact gcccgacgat gagcgccgca ccatcagcca
     7681 ccagttctac cagacccgtc aagatatggg gcagtgggtt cgtctggcgg cctccgtcct
     7741 ggcgcatcaa tcccgggctg cctcactggt cacggcgccc cacccggcag aggttcgctt
     7801 ccgccatcta gagctgatct ccactcgcgg tctccaggtg ctgatagtcc tggttttggt
     7861 gggcggcgaa gtccgccagc gcctggtcag cctcgatctg gttgcctctc aggagcagct
     7921 ttcagcgctc gccgaccatc tgaccaacct gttttccggc aaagacgccc tggggatgcg
     7981 caccctggcg acgcagctca ccggcatgga acaacagttc gcctactggg tgattgaaga
     8041 tatgtctgat attcaatcag cccccactgg cgaagttttt ctggacggcg tcagcaacgt
     8101 cctctccgaa cccgaattta tcggttcgga agacgcccgg agggcattgc agctattgga
     8161 agaacgctcg ctgctgcaag acctgctggc tcgttctgta ctgactgacc gtaccggcgg
     8221 tgtgcaggtc ctcattggcg gcgagggtac gcgcgaagaa ctgcgcccct tctcaatggt
     8281 tttggcctcc tatggcgccc caggtcttgc tatgggcacc ctgggcgtct tgggccccat
     8341 gcgcatgtcc tatggtcgca gcatttcaac agtccgcttt ttatccagcc tgcttagcaa
     8401 tctggttgct gaaacactga cggatgataa taaggaaggt ttagaggagg aatttgacta
     8461 atggtcgaag acaaacgtcg taagcacaaa cccgaagctc cgcccccggc cgacaccccg
     8521 gaagtgtcac ccgaagccgg agccgacgaa gaaataatct cagccgtgcg cccgcagcca
     8581 gctgaagaat cggagatgat cttagtgtct aaaaaagaac tggccgaaat gcaagcgcaa
     8641 ttggagtccg cccgccagga agctggcgaa aacatgaatg gctggcagcg tgagcgcgcc
     8701 gatttcctga actacaagaa acgcattgag cgcgatcagg tactgcaaac ccagtcaatc
     8761 aaggctgatc tggtcaagcg ctacctggat gttctggacg acctggagcg cgcgctccag
     8821 agtgaaaacc gcccacacgc gggcaaaagc gcccagtggg gctcaggcat cgagctcatt
     8881 tatcgtaaac ttcagggtat cctcgaagcc gagggcgttc agcgcatccc tgctgagaat
     8941 gaaatatttg atcccaatcg tcacgaggcc atcaccagcg aagacagctc aacgcatgag
     9001 agcggtcagg tgattggcgt agtgcgtcag ggatacatga ttggtgaccg ggttatccgc
     9061 ccggcgctgg ttcgagtagc taaataaaca ggaggtttct tatggctaaa attgtaggta
     9121 ttgatcttgg tacgacaaac tcggttgttg ctgtgatgat gggcggcgag ccggtcgtca
     9181 tccctaccgt tgaaggcgag cgcctgctgc cttccgttgt ggcggtcaac aaaaatcacg
     9241 agcgtctggt tgggcgttca gcccgcaacc aggcggtgat gaaccccgaa aacaccgtct
     9301 tttccatcaa gcgcttcatg ggtcgcaaat ttgatgatcc tgaagtgcag cgcaccatca
     9361 gccgcgtccc ctataaggtc aaggctgccg ccaacggcga tgtccgggtt gttctggatg
     9421 gcaaggaata ttccccgccg gaagtctcgg ccatgatcct ggctaaattg aaggccgatg
     9481 ccgaaacata tctgaacgaa cccgttactc aggccgtcat taccgtcccg gcttacttca
     9541 acgacgcaca gcgtaacgcc accaaagatg ctggtcgcat tgctggcctc gaagtcttgc
     9601 gcataatcaa cgaaccgaca gcttcctcac tggcctatgg tctggataag aagaagaacg
     9661 aagtgatcgc ggtctatgac ctcggcggcg gcacattcga tatctccatt ctcgatgtgg
     9721 gtgaaggcgt cttccaggtt cgctccacct ccggcgatac cttcctcggt ggtgatgact
     9781 ttgacatccg agtcatggac tattttattg atgaattcaa taagcaaagc ggcatggata
     9841 ttcgccagga ccgccaggcg ctccagcgtc tgcgcgaggc cgctgaaaaa gccaagattg
     9901 aactgtccac cgtcatgcag accgaaatca acctgcccta tctgacggct gatgcctctg
     9961 gccccaagca cctggtgatg accctcaccc gcgccaagct cgagcagttg accgaagatc
    10021 tggtcaagaa gagcctcgac cctgtccgcc gcgccctggc tgatgctggc ctcaaggcct
    10081 cggatattaa cgaagtagtc atggtcggcg gcatgacccg tatgccgctt gtccaggaaa
    10141 tggtccgccg tgaatttggc aaagaactcc accgcggcgt caatccggat gaggttgtgg
    10201 cggttggagc agccatccag gctgccgtct tgggcggtga agtcaaggac attctgcttc
    10261 tcgacgtgac ccccctgacc ctgtcgattg aaaccctggg cggcgttgcc acgccgatca
    10321 tcgagcgcaa caccaccgtc cccacccgca agagccaggt cttctccacc gcctcagacg
    10381 gccagacgac cgtagagatc catgtggttc agggcgagcg tcccatggct gccgacaaca
    10441 aatccctggg taaattccaa ctcgatggta ttcccccggc gccgcgcggc atcccgcagg
    10501 tcgaagtcac ctttgatatt gacgccaacg gtaccgtcaa agtaaccgca accgataagg
    10561 ctaccggtcg cgcccagcat atcactattg ccggttcctc cggtttgaac gagcaggaaa
    10621 ttgaacgcat gcgccgcgaa gctgaagctc atgcagatga agaccgccgc cgcaaggaga
    10681 tggtcgaagt ccgcaacaac gccgacaata tgatttacac caccgaacgc accctgcgcg
    10741 aagccggtga caaggtcccc gccgaccagc gcaaacaggc tgaagatgcc gttgcgaacc
    10801 tgcgctcgct tatggaaggt ggcgaccctg aagcggttcg taaagccacc gatcaacttg
    10861 gcgaactgat tcagaagatt ggtgcctcca tgtaccagca gcagcctccc actggcggac
    10921 ccacccccga ttccggcgct cctggcaatg gccaaacccc cggcggcgac gatgtggtgg
    10981 atggcgaatt ccgtaatctc taatccctac cggtgaaagt ggggcggtct gtacagaccg
    11041 ccccatttcc cgtttgaaag aaccgcatga catcccgtga ttactacgaa gtattgggcg
    11101 tccctcgaga cgcctcaccg gaagacataa aatctgcctt ccgcagcctt gcgcgccaat
    11161 atcacccgga tgtgaaccgg gaagctggtg ctgaagaaaa attcaaagaa atcaacgaag
    11221 cctacggtgt cctctcagat accgagaagc gctcgaccta tgatcgctat ggtcggtctg
    11281 gcctgggtga tatgggcggc atgcccgatt tcaccatgag tgattttgcc gatctctttg
    11341 gagaattttt cggctttggc ggtatgggcc ggcagcagcg gcgcaacatg cctcagcgtg
    11401 gcgccgatct gttatacacc ctcacgctga cttttgaaga atctgtattt ggtgttgaga
    11461 aagagattca ggttacccgg gacgaatcct gcagcaattg tcgcgggtct ggctccgagc
    11521 cgggcagcgg ccccacgcgc tgcaccacct gcggtggccg gggcgaagtt cgccaggtgc
    11581 gccagacgat cctcggttca atggtcacgg tcgcgccttg ccaggcctgc aatggccgcg
    11641 gcgaggtcat caccaatccc tgccatgtct gcaaaggccg tggcctggag cagcgcacca
    11701 cccgtaaact agttaccatc cccgccggag tggataacgg cacccgcctg cgccttgcag
    11761 gcgaaggcca gcccggcgcg aatggtggcc caaacggcgg cctgtatatc gacatttccg
    11821 tcaaacagca cgcctatttc cgccgccgcg gcgatgatat cttgctcaat ctgaatatca
    11881 acatcgctca ggccgccctg ggtgctgatg tcgaagtccc cacggtggat ggcccggttc
    11941 ggctctccat tccggcgggt acccagcccg gcaaaacctt taccatcaaa ggcaaaggcg
    12001 tgccgcgggt gcgttcttct tctcgcggcg atcagatcgt ggttgccaac gtggaagtgc
    12061 cctcccgcct taccccagag cagcggcagt tgtttgaaca gctttctcaa acgcttggaa
    12121 ccgaagtcaa accgcgtgag cgtggttttc tcgatctttt gaaggaggtc tttggtggct
    12181 gaagcccgtt ggttagaaat ctctctggtc gtcaatgatg aattagctga agcggttgct
    12241 gatgtgttgg gccgcttcgt cagtcagggt gtggtcatgg agcgcagcgt tgaatttaac
    12301 gatgccgagg atgaaggcac cccttatggc cctgtgcggg tttcaggtta cctgcctgtt
    12361 gatgaacagc ttgaagatgt acgtcagcag ctagagcagg cgctctggta tcttggccgg
    12421 attcaacccc tgcccgaacc ttcctaccgc accattgaag accaggattg gatggctgcc
    12481 tggaaacagc attaccgccc aatcccggtc ggtcaaaaat tgatgattct tcccgcttgg
    12541 ctcagtctgg acactgagga tcgcattgcc gtccggattg atcccagcat ggcatttggc
    12601 actggtacgc acccaaccac ccaattatgc atggccctgc tcgagaatcg agtcagcgaa
    12661 aacgaacctg tcattgacgt ggggtgtggg tcaggcatcc tgtccattgc tgcagtcaag
    12721 ttaggtgcag ttcgcgccct cgcggtcgat attgacccct tatccgtcaa agccaccctg
    12781 gaaaacgccc aggccaatgg cgtctcagag aagattgaag ctggcctggg ttctgtggct
    12841 gaaatattac agaaaaactt ctcgctggat cgtgctccgc tcgtcctggc aaacattctg
    12901 gccccggtta tcgtccgcct tctggatgcc ggtttggcgc agttggtact ccctggcggg
    12961 attttgatcc tctcgggtat catggtcgat caagccgatt cggtcatcga agccgctgcc
    13021 cgtcaccaac tcaccctcat cgagcagcaa accatgggtg attggatcgc cctggtgatg
    13081 caagccaaat aatcattcat ttgtaatagt tccgggcagg gcagcgtgtc tgccccgccc
    13141 ggatttttat tcctcgatag ttgaggaatt tatcattctc tgctttgcag cgcaggtctt
    13201 gttatagact ctttgctgct ccaattaccc ctattaggct gtgatcattt tcaattgctg
    13261 cgcccaacgc aatttcatca gcattgaggt aggttccaaa atctttcagc gccctggcta
    13321 aagcagcttg catcctctga tcaagcgtaa catctgcttc ccaccaccaa ttttgaaccg
    13381 tcaatcggcg cgacttctta tcaaattttg gttcaaaccg cgccacaaat cgttcaccgc
    13441 acagcactgg caacacataa tagccgtact ggcggatagt cgccggcttg tacacttccc
    13501 agatatagtc aaatccaaaa atccattgga taagcttgcg gtcccagagc aggttatcta
    13561 acggtgccag aaatgctgcc tctgtcgggt tgcagctgtt catttcgtca aggtgaacca
    13621 ggtctgccgt gcgcgcataa aatgtttgcc ggggaatttc ggtaactttt acaggtgtca
    13681 ccagtccagc ttctgtcagt cgtttcaacg cggcctggcg ctgaggcgac ttcgtcccca
    13741 caatccccag ccaatgctct ccgctgccta cctgcgccaa ccccagcccg ccgatacgcc
    13801 gcagtacatg ccagtcctga tacgcctcgt cgcttgcaaa ggggtccagg gcgtttaata
    13861 cagcttctgg cagcagattc ttgctcaggt caaatatccg ccgggtgttc acccggtgat
    13921 gaatccccag ctctccaatg gaaaataacg tttccagggt tgcccgcacc agccgcgttt
    13981 ccccccagaa ccagtctgcc cgtccatcat gatcgaaatc gattgaagac aatggcccgc
    14041 gggcttgaag ttctgaccgc accatttccg ccaactgcat cggccgatgc tcctcaccgc
    14101 cataatgaac ccgcatcagt gcccggcgcc tggcaaaatt cggccagtcc tgtgtggggt
    14161 agatgcacat caccttatca aacccatccc ataaccgccg ttcctgataa agcagccctt
    14221 ccagcagcac cggctggtac cccgccaccc gggactgcag cacaagctct gcgttccggc
    14281 caaacgatgg ctcaaccggg tcaaattgga tagaccccag gcgctcaaaa atttgcatca
    14341 ccccatcggt ccccgtcagt tgccgggctg gatataaaaa gtgatgcctg agcagaaaac
    14401 gccgcgcttg ttgaatggat aaagtgatca ttgcgttatt atagaacgtg tgttcgctcg
    14461 cgtcaagccc gccgtatatc ctgtcagtat cctgatgaat gataattgag taaatctgga
    14521 tggtgctgta caattatctc aggcaggtaa cctattccat ttctgacttt cattctggga
    14581 gggcatatga ataaccaatt ttgtgttggg caaagaagcg ttgcgcgcat tttgatcgcg
    14641 ctgattgttg ttgtactgct ggcggggctc attcaaccgg ttgcggctga ttctcaaatg
    14701 ccgtctgaac cggctccgca aattcaggcc gtcaataaca gccattggag caccgaattt
    14761 tatccccgcg gcgtcaatgg ctatgtctcc gatctggaga ttggtcctga tggatccctt
    14821 tacatcgccg gtcggttcta ccaggctggc acagctcagg taactggcct ggcgcgctgg
    14881 aaaaataatc aatggcaagc ttttggaaat tttacttctg catactattc aggaccctac
    14941 actggactgg ggtatgtaaa tgatattgcc atatcaggac aaaatattta tgtaggtgga
    15001 ttcttcagat attacaacca ctatgtttct gcaaacaatg tcgccgtgtg ggatgggact
    15061 cgctggtctc gcctgggata cggcattgat gatgaggtta cagcattaac gctggatagc
    15121 agtggtatcc tttatgccgg cacctattct ggcgatattt atagttggga tggtacatcc
    15181 tggacgattc agggcagcgc ggatgacaca atcaacgctt tagcctttga tgatgcagga
    15241 accttgtatg ccgctggctt ttttacaacg ctggatggtg tatcggtcaa ctatatcgcc
    15301 atgaaaagcg gcggaacctg gcagcccatg gcgggcggcg ccagccgtaa cgatggaaag
    15361 gacccgtggg ttagtgatat tgtcttctgg aacggcacat tgtatgcagg aggttggttc
    15421 gatgccatgg gcggaattcc agcttctcat ttagcctact ggcaagccgg aacctggcat
    15481 gccatcaccg ggctggatgc aggtgtttat gacctgcagc cagatggcaa taaattatgg
    15541 gttgggggaa ccctggatac catttcgggg cttcagacag gcaatctggc tgcctggaac
    15601 ggaatctcct gggaggtctt gcccgaacaa atttcgaacc aatccgtatc ggcaattgcc
    15661 gttaatggta ctcatttatt tgccggagga aatatctttg aagccgggca gactgggttc
    15721 tcgagtattg ctgagtggac gggtagtgcc tggaccgccc ttggagaagg acagggagcg
    15781 gcagatgaga tccagtcggt gctcctgccc cccgatcaaa actcgctgta tgtcggcggt
    15841 cgctttgatt caattggcgg tacccctgcc cattctattg ctcgctggga cggcagccag
    15901 tggcatactt taggggatgg gtttaagcga ggcactttag ttgcagatgt tcatgcctta
    15961 cttatggcag gtgatgtatt gtatgctggg gggtattttt catctagtgg ttccacgccg
    16021 ctgagcaaca tcgcacaatg gaatggcaat gcctggttgc cactgggtga gggtttagcg
    16081 agcacagaaa cttctggtag agtatatgcc ctgaccgctg atgatgctgg taatatctac
    16141 gccggagggt attttgataa atctggcgag ataattgtaa atcgtgtagc caggtgggat
    16201 ggcgcatcat ggcttccatt gggtgatggt ttggattata cagttcgcgc cctggcttat
    16261 gacacaacca atgatttact ttatgctgct ggtactccca atggaattcc tgaaccctct
    16321 ttcataggag tatcggtttg ggatggatca tcctggcagc cattgggcac cgagcctacc
    16381 ggctttgttt tcgcaatgaa aatatcccca gatggcatcc tgtatgccgg tattaatggt
    16441 tatgaactcg cttattggac tgataatgcc tggcatcgga tatcggagca ggcgatttcg
    16501 tcttc
//
LOCUS       DAOPVS010000031        16388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3600_length_16388_cov_58.569093, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000031 DAOPVS010000000
VERSION     DAOPVS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_16388_cov_58.569093"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1011)
                     /locus_tag="PKL11_04520"
     CDS             complement(37..1011)
                     /locus_tag="PKL11_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ12898.1"
                     /translation="MSALHFRTFENENDYPLLLEINLNSRQADNDYVMITLEDIAYAF
                     AHMDGLTPTEGVVIAFLANSPDMAVGYSRLGWYSSHPDNCLYYQVSFLRQEYRASHYW
                     PLMVAQNENLLRQIAARHPAVPQRFFQAWASDNQVEWMSVLENAGYQAVRRFNNMLYQ
                     LNEVPHIPLPADFDIRSVEPQHMHCIWEAQREMNAGLFENVAEDWLEEKYPAWLEDAS
                     HTPHLWQVAWVGDQLAGVVLTRIDPQENEALQRKRGYTEHIYVRPPWRQRGLASALIA
                     RSLQVLQAQGMAEAELGVDAENESAAFKLYQSLGYQTFSVDTWFRKAM"
     gene            1169..2146
                     /locus_tag="PKL11_04525"
     CDS             1169..2146
                     /locus_tag="PKL11_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ12899.1"
                     /translation="MEYRHLGRSGIRVSALSLGSWVTFHNQVDVKAAIDSMAAAYDAG
                     VNFFDNAEIYAGGKSEEVMGEALRQLGWRRSSYLVSTKFYWGLNNGINEKNTLNRKRL
                     LEAMDGSLKRFGLDYIDLIFCHRPDPTTPIEETVWAMSDLIERGKAIYWGTSEWSAGE
                     IIAAIEIAERHHLRKPVMEQPQYNLLWRDRFEKEYARLFRDYGYGSTTWSPLASGLLT
                     GKYNDGIPADSRGAVKGYEWLQKQLTNPEAIARVKALQVVANEIGVTLAHLALAWCLK
                     NPNVSTVITGASRVAQVQENMRAIEVVDRLTPEVMAQIDAALGKTEEEG"
     gene            complement(2205..3110)
                     /locus_tag="PKL11_04530"
     CDS             complement(2205..3110)
                     /locus_tag="PKL11_04530"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HNZ12900.1"
                     /translation="MNALSIGTAVPAKDFDAVVHSVFTRAANLKLKDSSRLLTLTRVD
                     EADLPQGIRLNTPPDFSFEMLHSGDQFTSRSGILSCVTARLTIDLRLAQRWKCNLKTL
                     AVSLEDVSTLDAWRRAVTIVNNYRLLTETQEPVAQSITCKMAQLTTGLVAATSSMDHL
                     AAIENAAGLIGLGPGLTPAGDDFLVGYLAGLWGTAGRIPERIQFVTNLGRAVARLSRH
                     TNEISGTYLTHAAHARVSSRLLDLARAIKQGQGSADLHPVTLLSLQIGHTSGLEATTG
                     LLQGLAVWNGRLAGHPLAHPDEGLP"
     gene            complement(3188..3544)
                     /locus_tag="PKL11_04535"
     CDS             complement(3188..3544)
                     /locus_tag="PKL11_04535"
                     /inference="COORDINATES: protein motif:HMM:NF023594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ12901.1"
                     /translation="MKKEIPIIAYKCKKCGRVHYPYHDRCLDCKSREFEKIKPQGNAR
                     LLTFTAIYNLPWGFDQRYLILGVAEFENKIKAMGQIKVDSIEDLKIGMQVHPSWEPVR
                     VQAGEDVYGLKFEPIK"
     gene            complement(3541..4731)
                     /locus_tag="PKL11_04540"
     CDS             complement(3541..4731)
                     /locus_tag="PKL11_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12902.1"
                     /translation="MRDVAIIGVGMIPFGRRDEDSLMDMLAYASLKALDDAGLGDKSV
                     NAVYVGNMGAGIVQHQTSIASSLVDRLSLLPAAADVVENGPASGGSAVKNGLLAVASG
                     YYDYVLVVGGEKMREVTGWRATDFVATMTHPQVEYPYGITLPGMAGMFTRLYMEKYGV
                     TREHLLAVTLKNQEMGALNPYAHVEMALDRKGIFDSAHAVVNNPVAADPLHLYDLCPV
                     SDGAAAVILCPLEMARNLPHAPVLIAGFGQATDTHALQEREDPTDLKAVTLAAQQAFE
                     MAKLKPSDIDVAELHDAFTILEIAESEHVGFFKKGEGGPAAAAGKTRLGGQLPINVSG
                     GLKARGHPVGATGVAQVVDIVFQLRHELPKNRQVKNARHGFTINFGGFGNNVTSFVLK
                     RIEP"
     gene            complement(4736..5425)
                     /locus_tag="PKL11_04545"
     CDS             complement(4736..5425)
                     /locus_tag="PKL11_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12903.1"
                     /translation="MPVDLELEAVLRPIQYPMPPYPPKVITLANGKQMVIRQAVREDV
                     PAILKSVHPCLFIERDYYDIVSARLYGELLAWYRYRAKSPYCLLGQVDGYLVGVVNGR
                     METEKVGMSYHTLAIDRGLRIGSHLFAAKMEYHMEYLGQEDVLIVAESPIGFRRWMIE
                     YPLEKTNIPHELGGGDSYRLTREIYLKARPRLVVGDRPVPQDLLAEAEAEIKFADDDT
                     IRERIAGLKGR"
     gene            complement(5437..6318)
                     /locus_tag="PKL11_04550"
     CDS             complement(5437..6318)
                     /locus_tag="PKL11_04550"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNZ12904.1"
                     /translation="MKFLDLTIPLGIATPPWPTYEPLQVKYFKRLAPNGANGQVITHS
                     NHLGTHLDGEIHFHTPGKDIASLDMDFLVHDAAVVDLSDVCGDYDIYTSKMVEDRVEV
                     REGDILIIHTGYHHFGWDMPTADEVRYMVMHPGPDREFAEWAKRKKLRWIGVDCGSAD
                     HPMNTIIRDWMPRQARQAEKVFKKKYGKPLAEFFDDSKYQLMHLEMFPHGIIHAECLG
                     GDIDLIVNRRVQIGFFPWRFVDGESCIGRCVAMLEDQEYASLMEKKAGMLHTKFGDAI
                     EATHVENLNKISRENLK"
     gene            complement(6373..7782)
                     /locus_tag="PKL11_04555"
     CDS             complement(6373..7782)
                     /locus_tag="PKL11_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HNZ12905.1"
                     /translation="MSPSEPRISDLFGKPLSVVNLGLESMAQSVSAQGIPVVHLDWRP
                     PQEGIPHLRQTKSGIDIEAANQAVVARIQQGQVSLRGMGIARKVIPGMHDHLFLHAGP
                     PITWERMCGPQRGAVMGALIYEGLANDEAEAARLAASGAIEFAPCHHYHAVGPMAGII
                     SPSMPVFILENKTFGNQAYCTQNEGLGKVLRYGGMGPEVTARLKWMETTLYPALDRAL
                     QTLPNGIDIKSLIAQALHMGDECHNRNRAATSLFLRAIGPALARTNQDNEILARIIEF
                     IDRNDHFFLNLSMPTGKALLEPAEGIAGSTIITVMARNGTDFGIRLASFPERWFTAPA
                     GKVQGLYFPQFSEQDANPDIGDSTITETAGYGGMAMAAAPAIVQFVGGSPQLATQTTL
                     EMYEITSGEHQNLTIPALNFRGTPLGIDVRRVMETGILPQINTGIAHKDPGIGMVGAG
                     ILRAPEKCFKDAFDFLVNV"
     gene            complement(7795..8031)
                     /locus_tag="PKL11_04560"
     CDS             complement(7795..8031)
                     /locus_tag="PKL11_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12906.1"
                     /translation="MSFIQKFITTVFSKKLADQIKAESQSWIMRCKTCGLERSIWEIG
                     GVRYRAAGNPSRKYPCPTCGRWTWHSVERKQNSN"
     gene            complement(8045..9568)
                     /gene="fdrA"
                     /locus_tag="PKL11_04565"
     CDS             complement(8045..9568)
                     /gene="fdrA"
                     /locus_tag="PKL11_04565"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HNZ12907.1"
                     /translation="MIVKAVIKPGEYHDSVSLMLVARELASQPGVQDAAVVMGTEANK
                     SILDGSGLFTAELAAATANDLVLVVSAVDEPGARQALEKADGLLKKKAAPESVGEFKP
                     KTIRSAISGQPDANLAVISVSGRFAADEAWEALRHGLHVLLFSDNVSLADEIALKRYA
                     VEHGLLLMGPGAGTAILNGVALGFANVLPHGPVGIVSAAGTGLQEVSTLLARQGVGIT
                     QGIGTGGRDLKEEVGGLMMLAGLQALQTDPDTQVIVLVSKPPAAAVADKIVAQAQQCG
                     KPTVVCFMGDRQARRAAKTLSFVQTLEEAAQAAAARAGAQPAPRQIPAVNPVHHTPGK
                     LRGLFSGGTLCYEAQVIWRDLLPEPVLSNAPLDHANQMANSLDSHGHCAVDLGEEEFT
                     VGRPHPMIDNDLRIRRLLQEARDPEVAVIMLDVVLGYGAHPDPASELAPAIQKALEIA
                     HNAGRKLSVITSVTGTQGDPQGLTAQIQKLETAGAIVCPSNAAAARFAADLILGSSR"
     gene            complement(9570..11927)
                     /locus_tag="PKL11_04570"
     CDS             complement(9570..11927)
                     /locus_tag="PKL11_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNZ12908.1"
                     /translation="MGKTTTPKKIGPVGENKPRIDGPEKVTGAAQYADDIQFGNRLLH
                     ARVKRSPHPHARIKKVDVSRALALPGVKAVVTGEDFSGFIGLYLKDRHIFCRDRVRYV
                     GDPVAGVAATTPEIAEKAVNLIDVEYELLEPVLDPEFGASPNAPLLHPDLGTYAVANF
                     IMPQPGSNISNHFKIRKGDVDAAWGKCAAIIERRYHIPHIQHVPIEPHVAIAKVDETG
                     SITLWGSSQSPFAQRDLIAEALGISQSKMRVIAPAVGGGFGGKAGVSMESLAVAIATK
                     VRGYAVKLLLTREEEFYTAFVRQGLVAYFKIGCDQNGKILAMENTFYWDGGAYTEYGV
                     NITRAGGYSCTGPYEVPNIRTDSYCVYTNHPVGGPMRGFGMPEIHAGLEQCIDELALE
                     MGLDAVELRRRNCLKGGSILPTGGQMHPTGLSECLEQAAAAIGWGQKAPTSAPNKRRG
                     KGLALMWKAPAMPGNAGSSAWIELSEDGMLTLGVGCQDLGQGAFTVVAQMAAAAFGVP
                     YESIRLAAPVDTRYSPYEWQTVASRITWSTGNAVIQAAHDARHQVLELVSKAWNENVE
                     DLDIVNGVVVSYKTEREIPVKDFAIKGLPKPDGKGWMGGPIIGRGNFMPTDVTGLDPE
                     TGQGDHAVVHFTTGAQAVELEVDLDTGRIEIIKGVAAFDVGKAINPDLVKAQMEGGLI
                     QGISSALFECLMLKDGKVLNPSFVDYRIATSADIPRSLESIVVEVPQEDGPWGARGVG
                     EHSMVPTIPAIANAIYDAIGIRFDGPPFTAERIYLAMLDAGIVKA"
     gene            complement(11957..12436)
                     /locus_tag="PKL11_04575"
     CDS             complement(11957..12436)
                     /locus_tag="PKL11_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ12909.1"
                     /translation="MAFHQINITVNGHSEQVTVASNTTLMRMLRETLALTGTKNGCSA
                     GECGACTVLLNGEPVNSCMVLAVECDGAEVITVEGLAGDHQMDPVQESIVNSGGVQCG
                     FCTPGILISARALLNRNPDPSEEDIKNALVGNLCRCTGYVRIIESIKKASRVKVSRP"
     gene            complement(12488..13384)
                     /locus_tag="PKL11_04580"
     CDS             complement(12488..13384)
                     /locus_tag="PKL11_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNZ12910.1"
                     /translation="MVTHTHPTLPEFDYIQPESLAQASAFLAEHASEARPFLGGTDIF
                     VRMRDGFFTPKFLVNVKHLPGMNDIQFDPQTGLTIGAAVNMNRVAANPEVNAHYPLLV
                     EACQSVASYQLRNRATIVGNICNASPAGDTLGACLALGGLLQVHSLEGRRQEPLTGFF
                     LGPGKTRLKAGDIVTGISFPLQPQGSAGAYLKLGRNKISDLSIVGVTVLACPDSRLPS
                     GVRFHIVLASVAPVPLVAVEAETCLGAQAVTPESIHQAARLASEACTPIDDIRSSARY
                     RKLMVKNLTETALTNVWHKLQK"
     gene            13654..>16388
                     /locus_tag="PKL11_04585"
     CDS             13654..>16388
                     /locus_tag="PKL11_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ12911.1"
                     /translation="MDEVEKLEKAIAGLEIQRAILGDDVVKAAVSALREKISLLQAEI
                     QPERRKRKIASVLFADVSGFTAMSERMDPEDVSDLMNRLWERIDQVIIDHGGRIDKHI
                     GDAVMALWGTEAVREDDPERSVRAALAVQIELAKITRGEGFLDARFRGEAQELAIRVA
                     VHTGPVLWGQTGTTGEYSAIGDTVNTASRMQHQAPLGGVVVSAATALHIQGLFDLEVL
                     EPLKAKGKSEPVRIFRVLRPKPRAYAMRTRGVMGISTPLVGRDAELNEMVNVLEHCVQ
                     NQSCCMLTITGEAGIGKSRLQYEFEKCVEQSGRRYRILRGRAGQDMVNQPYALLRDLF
                     SNAFQIQDSDTREEVWQKFEQGIATPYRLPEQDKPLQVMQAHFIGQLLGFNFNSSAHL
                     VGAIKDAKQLHDRALVYLIAFFRAMAEQEPVIIFLDDLHWADESSLDHLIHIFRALNH
                     LRILFVCMARPTLFERRPGWGSGKGHTQIKLQPLAFEASQKLLEHILQKLEDIPENLR
                     DMIIQNAEGNPYYIEELVKMLVENGVIIQTGEQWRVVSERLQVEQIPSTLAGILQARL
                     DSLTELERDTLQCSAVVGQTFWNQAVQTLNPDAFAELDETLAELNQRELIEPSEVSAF
                     ADSTEYVFRHHILYGVTYDSVLKSLRRQYHAQIARWLIAHEGERAGEFSGLIADHLEK
                     AERYDEAVVYLRKAGERAIQSYANNEGVQYFSRAIHLTAEDDYAAQYQLRLARENLND
                     LLGERAAQAQELDALDALNDHFDASQQAEIAIRRANLHLVTGNYAIGLEAARKATALA
                     EKSKDSRHLATALMLQAQALWRLNQRPEAHALLLRALELASTVEQAETRSDVLRMLGN
                     LAYDQDIYAEARSYFEQSLEICRQIGFRRGECAVLNSLGILLNRINRGSDEALGC"
BASE COUNT         3715 a         4292 c         4738 g         3643 t
ORIGIN      
        1 gatggatgtt ttgagggatt aagggcaggc gccatgttac atcgctttgc ggaaccaggt
       61 atcaacgctg aaagtctggt atccgaggct ctggtagagt ttgaacgccg cgctttcgtt
      121 ttcagcatct acgcccagtt ctgcttctgc catgccctgg gcttgtaaga cctgtaaact
      181 acgcgcaatc agagcgctgg ccaatcctcg ctggcgccag ggtggacgaa catagatatg
      241 ctctgtgtag ccgcgctttc tttgaagcgc ttcgttctcc tgggggtcaa ttcgggttag
      301 caccaccccc gccagttgat cgcctaccca ggccacctgc cataaatggg gtgtatggga
      361 ggcgtcttca agccaggccg gatatttctc ttccagccaa tcttcagcaa cattttcaaa
      421 aagaccggca ttcatttctc gctgggcttc ccaaatacaa tgcatgtgtt gtggttccac
      481 ggagcgaata tcaaagtctg caggaagcgg aatatgggga acttcgttca gttgatacaa
      541 catgttgtta aagcgacgca cagcctggta gccagcattt tcaaggacag acatccactc
      601 aacttgatta tcagaagccc aggcctggaa aaagcgctgc ggtaccgcag ggtgtctggc
      661 ggcgatttgc cgcaagagat tttcattttg cgccaccatg agcggccaat agtgcgacgc
      721 gcggtattcc tggcgcaaaa agctgacctg ataatataaa caattatccg gatggctgga
      781 ataccagccc agacggctgt agccaaccgc catatccggt gaattagcca ggaatgcaat
      841 gacaacgcct tcggttggag tcaaaccatc catatgggcg aaggcataag caatatcctc
      901 cagcgtaatc attacataat cattgtctgc ctgacggctg ttgaggttaa tctcaagcag
      961 cagcggataa tcgttttcgt tctcaaaggt acggaaatgt agagctgaca tttagcgctc
     1021 gattcgtttg tggggataac ccccattata cctgctctat aaatcaacag ccggcattag
     1081 aaaaaacgcc agccagattt tgacgattgg aaaagccata accatttatg ttagaattgc
     1141 actcaacaac ataggaagga gcataaaaat ggaatatcgg catcttggac gtagtgggat
     1201 ccgtgtttcg gccttatcgt tagggtcgtg ggtgacgttc cacaatcagg tggatgtcaa
     1261 agcggcgatt gactcaatgg cggcagcata tgatgcgggc gtcaactttt ttgataacgc
     1321 agaaatttac gcgggtggaa aatcggaaga ggtgatgggc gaggcgctgc ggcagttagg
     1381 ctggcggcgt tccagttatc tggtctcaac caagttctac tggggtttga ataatgggat
     1441 caacgaaaag aacaccctca accgcaagcg gctgctggaa gccatggacg gctcactcaa
     1501 gcgttttggg ttggattaca ttgacttgat tttctgtcac cgacccgacc ccaccacccc
     1561 gattgaagag acggtgtggg cgatgagcga cctgattgag cggggcaagg cgatttactg
     1621 gggcacgtct gaatggtctg cgggtgagat tatcgcggcc atcgagattg ccgagcggca
     1681 tcatctgcgc aagccggtga tggaacagcc gcagtacaat ttgctctggc gcgaccggtt
     1741 tgaaaaggaa tatgcccggc tattccggga ttatggctac ggttcgacca cctggagccc
     1801 gctggcctcg ggtctgctga cgggcaaata taacgacggc atcccggcag attcacgggg
     1861 ggcggtgaag gggtatgaat ggctacagaa gcagttgacc aaccccgagg caattgccag
     1921 ggtgaaggcg ctgcaagttg tggcgaatga gattggcgtg acgctggcgc acctggcttt
     1981 agcctggtgc ttgaagaacc ccaatgtgag cacggtgatc accggggcga gccgggtggc
     2041 gcaggtgcag gaaaatatgc gggccatcga agtagtggat cggctgacgc ccgaagtgat
     2101 ggcacagatt gatgctgcgc tgggcaaaac agaagaagaa ggttaagtcc ggcaacagat
     2161 aataacaacc aaaggctgct ctcaaacgag ggcagccttt tgagttatgg taagccctcg
     2221 tcaggatgag ccaaggggtg acctgctaag cggccattcc agacagcaag tccttgcaat
     2281 aacccggtgg tggcttccag gcctgaggtg tgaccaatct gcaaggataa caaagtcacc
     2341 gggtgcagat cagccgagcc ctggccctgt ttgattgcac gagccagatc aaggaggcgg
     2401 ctggacaccc gggcgtgggc ggcgtgggtt aagtaggtgc cgctgatttc attggtgtga
     2461 cgggaaaggc gggcaaccgc tcgccccagg ttcgttacga actggatgcg ttccgggatt
     2521 cttccggcag tgccccataa ccctgccagg tagccaacca aaaaatcatc acctgccgga
     2581 gtcagacccg gcccgaggcc aatcaacccg gcagcattct caatcgcagc aaggtgatcc
     2641 atgcttgatg tggcagcaac cagaccagtc gtcaattggg ccatcttaca ggttatagac
     2701 tgcgcaacgg gctcctgtgt ttcagtaaga agccgataat tattaacaat tgttacagca
     2761 cgccgccacg catcgagggt tgaaacatct tccagactga cagccagagt tttcaggtta
     2821 catttccagc gctgcgccag acgcaggtca atggtcaggc gggcagtaac acaactcagg
     2881 atacctgagc gggaagtgaa ttgatctcct gaatgcagca tctcgaatga aaaatctggc
     2941 ggcgtgttca ggcgaatgcc ctgaggcaag tcggcttcat caacccgggt aagcgtgagc
     3001 aggcgcgaac tatccttcaa tttaaggttg gctgcccgag tgaacacact atgaaccacg
     3061 gcatcaaagt ctttagctgg aaccgcagtg ccgatgctta aagcgttcaa accatctgcc
     3121 cttcagaaga taaggcgcag tccgggttac ctgcccggac tgcgcacagg gtcaatttac
     3181 aatccggtca tttgatcggc tcaaatttaa gcccatatac gtcttcgcca gcttgaaccc
     3241 gaacaggttc ccaactggga tgtacttgca tcccaatttt caggtcctca atcgaatcga
     3301 ccttgatctg ccccatggcc ttgattttat tttcaaactc agccacaccc aagatcaggt
     3361 aacgttgatc gaacccccat ggcaaattgt aaatggccgt gaaagtgagc aatctggcat
     3421 tgccctgagg tttgattttt tcaaactcgc ggctcttgca atccaggcac cggtcatggt
     3481 aagggtaatg gacccgacca cattttttgc atttataggc gatgattgga atttcttttt
     3541 tcatggctca atcctcttca acacgaagga ggtaacgttg ttgccaaagc cgccaaagtt
     3601 aatggtaaag ccatgccggg catttttgac ctgacggttc ttgggcaatt cgtgacgaag
     3661 ctggaatacg atatccacaa cctgggcaac gccggtagcg cccaccgggt ggccgcgggc
     3721 tttcagccca ccggaaacat tgatggggag ctgccctccg aggcgggtct tgccagccgc
     3781 cgcggctggg ccaccttcgc cctttttgaa gaagccgaca tgctcgcttt cggcgatctc
     3841 caggatagtg aaagcatcgt gcagttcagc cacatcaata tctgagggtt tcagtttggc
     3901 catctcaaaa gcctgttgag cggcaagggt aactgcttta agatcggtgg ggtcttcgcg
     3961 ttcctggagg gcatgtgtat ccgtcgcctg cccaaaacca gcgatcaaca cgggggcatg
     4021 gggcaggttc ctggccattt ccagtgggca gaggataacg gccgcggcgc catcgctcac
     4081 cgggcaaagg tcatacaggt ggagcggatc ggcagcaacc gggttgttga ccacggcatg
     4141 tgcgctgtcg aaaatgccct ttcgatccag cgccatttca acatgggcat agggattcaa
     4201 agcgcccatt tcctgatttt tcaatgtaac agccagcaga tgctcacggg ttacgccata
     4261 tttttccatg tacaaccggg tgaacatgcc agccatgccg ggcagggtga tcccgtatgg
     4321 atactcaact tgcgggtggg tcatggtggc aacaaaatcg gtggcgcgcc agcctgtgac
     4381 ctcacgcatt ttttcgccgc ccacgaccag tacgtaatcg tagtaaccgg aggcgacggc
     4441 caaaaggccg tttttgaccg cggaaccgcc agaagccggg ccgttctcga ctacatccgc
     4501 tgcagcgggc agcaggctaa gacggtcgac cagtgaactg gcaatggagg tttgatgctg
     4561 gacaatgcct gcacccatat tacccacata aaccgcattg acagatttat ctcccaggcc
     4621 ggcgtcatcg agggctttca aggatgcgta ggcgagcatg tccatcaggc tgtcttcatc
     4681 acgccgcccg aaggggatca tgccaacccc gataatggca acatctctca tggtttcacc
     4741 tgcctttcag accagcaata cgctcgcgga tggtgtcatc atctgcgaat ttaatctcgg
     4801 cttcggcctc ggcaagtaaa tcctgaggca cggggcggtc gccaaccacc aggcgaggcc
     4861 tggctttgag gtagatttcg cgggtcaggc gataggaatc cccgccgccc aactcgtgcg
     4921 gaatgttggt cttttcgagc gggtattcaa tcatccaacg gcggaatcca atggggcttt
     4981 cagcgacaat caggacatct tcctgcccga ggtattccat gtggtattcc attttggcgg
     5041 cgaacaagtg ggagccaatc cgcagcccac gatcaatggc cagggtgtgg tagctcatgc
     5101 caactttttc ggtttccatg cgaccgttga ccaccccgac cagataccca tcgacctggc
     5161 ccagcaggca gtacgggctt tttgcccggt agcgatacca ggccagcagt tcgccataga
     5221 gccgggcact gacaatatca taatagtcgc gttcgatgaa caaacaggga tgcacggatt
     5281 tcaggatcgc cggaacatcc tcacggaccg cctgacggat gaccatctgt ttaccattgg
     5341 ccagtgtgat gaccttgggt ggatatggcg gcatgggata ttggattgga cgtagaacag
     5401 cttcgagctc taaatctaca ggcatagccc acactcctat ttcagatttt ctcggctaat
     5461 tttgttgagg ttttcgacat gggtggcttc aattgcgtcg ccgaattttg tatgcagcat
     5521 gccagctttt ttctccatca ggctggcata ttcctgatct tcgagcattg ccacacagcg
     5581 tccaatgcaa gattcgccat ccacaaaacg ccaggggaag aaaccaatct ggacgcgacg
     5641 gttaacaatc aggtcaatat cgccgccgag acactcagca tggataatgc cgtgggggaa
     5701 catttccaga tgcatgagct gatatttgct gtcatcaaaa aattcggcca ggggtttgcc
     5761 atatttcttc ttgaagactt tttcagcctg acgcgcctgg cgcggcatcc aatctcggat
     5821 gattgtattc atggggtgat cggcgctgcc gcagtccacg ccaatccagc gtaatttctt
     5881 gcgcttggcc cattcggcaa actcacgatc ggggccgggg tgcatgacca tgtagcgcac
     5941 ctcgtcagca gtaggcatat cccagccaaa gtggtggtag ccggtgtgga tgatgaggat
     6001 atcgccctcc cgaacttcaa cccggtcttc gaccatcttg ctggtgtaga tgtcatagtc
     6061 gccacagaca tcacttagat caacaacggc agcatcgtga accaggaagt ccatatccaa
     6121 cgaggcgata tctttgccgg gtgtatgaaa gtggatctca ccatccaggt gggtgcccag
     6181 gtggttggag tgggtgataa cctgtccatt ggcaccgttg ggggccaggc gcttgaaata
     6241 cttgacctgc aaaggctcat aggtgggcca gggtggggtg gcgatcccca aaggaatggt
     6301 taaatcgaga aatttcatat caggttctcc tttcggaatt gggggagttg agtgattggg
     6361 cccggtaaat ggctaaacat tcaccagaaa atcaaaggca tctttgaaac acttctcggg
     6421 ggcacgcagg atacccgcgc caaccatgcc gatgccgggg tctttgtgcg caatgcctgt
     6481 gttgatttgt ggcaggatgc cggtttccat cacccggcgg acatcaatac ccagcggtgt
     6541 gccgcggaaa ttgagcgcgg ggatggtgag attttgatgc tcgccagagg tgatttcgta
     6601 catttcgagt gtggtctgag tagcaagctg cggactgccg ccaacaaatt gaacgatggc
     6661 tggggcggca gccatggcca tgccgccata gccggcggtt tcagtaatgg tgctgtcgcc
     6721 gatatcggga ttggcatctt gctcgctgaa ctgagggaag taaagccctt gcaccttacc
     6781 ggccggggcc gtgaaccagc gctccgggaa agaagccaga cggatgccaa agtcggtacc
     6841 attgcgggcc atcacggtga tgatggtgct gccggcaatt ccttcggcgg gttcaagcag
     6901 ggctttgccg gtgggcatgc tgagattcaa gaagaaatga tcattgcgat caatgaattc
     6961 aatgatgcga gccaggattt cgttgtcttg attggtacga gccaacgcgg gtccaatggc
     7021 ccgcaggaat aacgacgtgg cggcgcggtt gcggttgtgg cattcatccc ccatgtgcag
     7081 ggcctgggcg atcaggctct tgatatcaat gccgttgggc agggtttgca gggcacgatc
     7141 cagcgccggg tacagggttg tttccatcca cttcaaacgg gcggtaactt ctgggcccat
     7201 gccaccgtag cgcagcacct tccccaggcc ttcattttgg gtgcagtagg cctggttgcc
     7261 aaaggttttg ttctcgagaa taaagaccgg catggaggga gaaatgatgc ctgccatggg
     7321 accaacagca tgatagtgat ggcaaggtgc aaactcgatg gctccagaag cagccaagcg
     7381 agccgcttct gcttcatcat tggccagccc ctcgtaaatt aatgctccca tcaccgcacc
     7441 acgctgcggg ccgcacattc gctcccaggt aatgggaggt ccggcgtgca ggaacaggtg
     7501 gtcgtgcatg ccggggataa ccttgcgggc gatgcccata ccgcgaagag aaacctggcc
     7561 ctgttgaatc cgtgccacca cggcttgatt ggcggcttcg atatcaatgc cggatttcgt
     7621 ctggcgtaag tgtggaatgc cttcctgggg tggccgccaa tcgagatgaa ccaccgggat
     7681 gccctgggca ctgacagact gggccatact ttccaaaccc agattcacca ccgacagggg
     7741 cttgccaaaa aggtcgctga tccgaggttc ggatgggctc ataatcgatt tcctttagtt
     7801 cgagttttgt ttgcgctcaa cactgtgcca ggtccagcgg ccacaggttg gacaggggta
     7861 cttgcggctc gggttgcccg ccgcccgata gcgcacgccg ccgatctccc agattgagcg
     7921 ctctaaacca caggttttac agcgcatgat ccaggattgg gactcggcct tgatctggtc
     7981 agccagtttt tttgaaaaca cggtggtgat aaacttttgt atgaaactca tcctcaatct
     8041 cctcttacct ggacgatccc aggatcaaat cagccgcaaa ccgggcggca gcggcgttgc
     8101 tggggcaaac tatggcacca gcggtttcca gcttttggat ctgggcagtt aatccctgag
     8161 ggtcaccctg cgtgcctgtg acagaggtta tcacgctcag tttgcgcccg gcattgtgtg
     8221 ctatttccag agctttctga atggccgggg ccagttcact ggcggggtca gggtgggcgc
     8281 cgtaccccag aaccacatcc agcatgatga cggcaacctc ggggtcgcgg gcttcctgta
     8341 acagacggcg gatacgcaaa tcattgtcaa tcatcggatg cggacggccg acggtgaact
     8401 cttcctcacc cagatcaacc gcacaatgcc cgtgactgtc cagcgaattc gccatctgat
     8461 tggcatgatc cagcggtgca ttggagagca ccggctcggg cagcaaatct cgccagatga
     8521 cctgggcttc gtagcataat gtgccaccgg agaatagccc ccgaagctta ccgggggtat
     8581 gatggactgg atttaccgca ggaatctgac ggggggcggg ttgtgcccct gcccgcgctg
     8641 cggcagcctg ggcggcttct tccagtgtct ggacaaagct caacgttttg gctgcccggc
     8701 gcgcctggcg gtcgcccata aagcagacca ccgtcggttt gccgcactgc tgcgcctgag
     8761 cgacaatctt gtcagccaca gccgccgccg ggggtttgct gacgaggaca atcacctgcg
     8821 tgtctgggtc cgtctgcagg gcctgcagcc cggcgagcat catcaggcca cccacctctt
     8881 ctttgagatc acgtccaccc gtgccaatac cctgagtaat gccaacaccc tgacgagcca
     8941 ggagcgtgct gacctcttgc aggccggtgc ccgcggcgga gacaatccca accgggccgt
     9001 gtggcagcac gttggcaaaa cccagcgcca caccattgag gatggcggtt ccggcgccgg
     9061 ggcccatgag cagcaagcca tgctctaccg catagcgctt gagcgcaatt tcatcggcaa
     9121 gcgagacatt atcgctgaaa aggagcacat gcagaccatg ccgcaaagcc tcccaggctt
     9181 cgtcggcggc aaaacggccc gagactgaga tcacagccag gttggcgtct ggctgcccgg
     9241 agattgcgct gcggatagtt ttcggcttga actcgcccac cgattccgga gcggccttct
     9301 ttttcagcaa gccgtcggct ttttcaagcg cctgccgggc gccaggctca tccacagcgc
     9361 tgacaaccag caccaggtca ttggcggttg ccgcggccaa ttcggcagtg aataacccag
     9421 aaccatccag aatggattta ttggcttctg tgcccatgac cacagcggca tcctgaacgc
     9481 cgggttggct ggcaagttca cgggcgacca gcatgaggct gaccgagtca tgatattcac
     9541 cgggtttgat gactgctttg acaatcatgt tatgccttca caatcccggc atccagcatc
     9601 gccagataaa tcctttcggc tgtaaagggc gggccgtcga aacgaatgcc gatggcatcg
     9661 taaatggcat tggcaattgc cggtatggtg ggcaccatgg aatgctcacc aacgccgcgg
     9721 gcgccccagg ggccatcctc ctgcgggact tcgacaacga ttgactcgag agaccggggg
     9781 atatcggctg aggtggcgat acgataatca acaaaactgg gatttaggac tttgccatct
     9841 ttgagcatga ggcattcaaa taaggcggag ctaattccct ggatcaggcc gccctccatt
     9901 tgcgctttga ccaggtcggg attaatggcc ttgccaacat caaaggcagc tacgcctttg
     9961 atgatttcaa ttcggccagt gtccaggtct acttccaact cgactgcctg ggctccggtg
    10021 gtaaagtgaa ccacggcatg gtcaccctgg ccggtttcgg ggtccaggcc ggtcacatcg
    10081 gtgggcataa aattgccccg cccgatgatc ggcccgccca tccagccttt gccatctggt
    10141 ttgggcagac ctttaatggc gaaatctttg actggaatct ctcgttcggt cttgtaagaa
    10201 acgacaaccc cgttaacaat atccagatct tcaacattct cattccaggc tttagagacc
    10261 agttcgagga cctggtggcg ggcgtcgtga gccgcctgaa tcactgcgtt gccggtgctc
    10321 caggtgatgc ggctggctac ggtttgccac tcgtaagggc tgtagcgtgt gtccacgggg
    10381 gcggcaaggc ggatgctttc ataggggacg ccaaaggcgg ccgcggccat ctgcgccacg
    10441 acggtgaatg cgccctggcc aagatcctgg cagccaaccc ccaaggtgag cataccatct
    10501 tcagacagtt caatccaggc gctggagcca gcattgccgg gcatggccgg ggctttccac
    10561 atcagcgcaa ggcccttgcc acgccgtttg ttgggggcgc tggtcggcgc tttctgtccc
    10621 cagccaatgg ctgctgcggc ctgttccaga cattcggaga ggccggtggg gtgcatttga
    10681 cctccggttg gaagaatgct gccgcccttc aggcagttgc ggcgccgcag ctccacggca
    10741 tccaggccca tttcaagggc cagttcatcg atacactgct ccaacccggc atgaatctcg
    10801 ggcatgccaa agccgcgcat gggcccccct acaggatggt ttgtatagac gcaatagcta
    10861 tcggttcgaa tatttggcac ttcgtagggg ccggtacaac tatagccgcc cgcgcgggtg
    10921 atgttgaccc cgtattccgt ataggcgccg ccatcccaat agaaggtgtt ctccattgcc
    10981 aggattttgc cattctggtc acagccgatc ttgaaatagg ccaccagccc ctggcgcaca
    11041 aaagcggtgt aaaactcttc ttcacgggtg agcagcagct tgaccgcata gcccctgact
    11101 ttggtggcga tggccaccgc cagggattcc atgctgacgc cagctttgcc gccaaacccg
    11161 ccccccacag cgggggcaat cacccgcatt ttgctctggc tgatgcccag ggcttcggcg
    11221 atcagatcac gctgagcgaa gggggattga gaactgcccc acagcgtgat gctgcccgtc
    11281 tcatccactt tggcgatggc gacatggggc tcaatgggga cgtgttgaat gtggggaatg
    11341 tggtaccggc gctcaataat ggcggcacat tttccccagg cagcatcgac atctcctttg
    11401 cgaattttga agtgatttga aatgtttgag cctggttgag gcatgatgaa gttggctacc
    11461 gcataagtgc ccaggtcagg atgaagcaag ggagcatttg ggctggcgcc aaactcggga
    11521 tcaagaacag gttcaagcag ttcgtattcc acatcaatca ggttgactgc tttttcggca
    11581 atttccgggg ttgtggccgc cacgccagcg accgggtcgc cgacataacg cacacggtca
    11641 cggcagaaaa tgtggcggtc tttcaggtac aggccaataa agcccgaaaa atcttccccg
    11701 gtaacaactg ccttgacacc cggcagggca agtgcgcgag aaacatcgac ttttttgatg
    11761 cgtgcgtggg ggtgcgggct gcgctttacc cgggcatgaa gcagccggtt accaaactga
    11821 atatcatcag catactgcgc tgcgccggtc actttttcgg ggccatcaat gcgtggtttg
    11881 ttctcaccga cagggccaat ttttttaggg gtggttgttt ttcccatcgt tgcctcttca
    11941 gaaaatgaac gaatgatcag ggcctggata ccttcacacg agaagctttc ttgatgctct
    12001 caatgatgcg aacatacccg gtgcagcggc agagattgcc taccagggcg tttttgatgt
    12061 cttcttcgct ggggtcgggg ttccgattca gcagggcacg ggctgagatc aggattccgg
    12121 gggtgcaaaa gccgcattgg acgccgccgg aattgacaat gctctcttga acagggtcca
    12181 tctggtggtc acccgccagg ccctcaaccg tgatgacctc ggcgccatcg cattcgacag
    12241 ccaggaccat gcagctattg acgggttcgc cgttcagaag gacggtgcag gcgccgcatt
    12301 caccggctga gcagccattt ttcgtaccgg ttaaggcgag agtctcgcgt aacatgcgca
    12361 tgagggtggt attggatgca accgtgacct gttctgaatg accgttaacg gtgatgttga
    12421 tctggtggaa agccatatat cctccgaaga attgtccctg gaaagccaat ccaatatttt
    12481 tgaggggtta tttttgcagt ttatgccaaa cgttggtgag tgcagtttcg gtcaggtttt
    12541 tgaccatcag cttgcggtag cgggcgctgc tgcgaatatc atcaatgggg gtacaggctt
    12601 cgctggccaa acgcgcggcc tgatgaatgc tttcgggtgt gaccgcctga gcgcccagac
    12661 aggtttctgc ttctacggca accagcggca ccggggcaac cgatgccagc acgatgtgaa
    12721 aacgcacccc ggacggcagg cgggaatccg gacaggcgag taccgtcacg ccgacaatcg
    12781 acagatcgct gattttgttg cgccccagct tgaggtaagc gccagcgctc ccctggggtt
    12841 gtaagggaaa gctgatgcca gtgacgatgt caccggcttt cagcctggtt ttaccggggc
    12901 ctaagaagaa gccggtcagc ggctcctggc ggcggccttc aagactgtga acttgcagca
    12961 acccgcccag agccaggcaa gctccaagtg tgtctccggc gggggaagca ttgcaaatat
    13021 tgccaacaat ggtggcgcgg ttgcgcaact ggtagctggc aaccgactgg caggcctcga
    13081 ccagaagagg gtaatgggca tttacctcgg gattggcggc tacccggttc atgttaaccg
    13141 ccgctccaat ggtcagaccg gtttgcgggt cgaactggat atcgttcatc ccgggcaaat
    13201 gcttgacatt taccagaaat ttcggggtga agaagccatc ccgcatacgg acaaaaatgt
    13261 ccgttccacc cagaaacgga cgcgcttcgc tggcatgctc ggccagaaag gcgctggcct
    13321 gggctaaact ttccggttga atataatcaa actcgggtag ggttggatgc gtgtgtgtga
    13381 ccatacggtt tcctcgcgcc agattaaatt gatgagatca agagggagag aacaataaag
    13441 cgcagatgcg ctttttgtgg gggaaatatc atcagatgat attattatgg cacagatttt
    13501 gtttttggga taccaaaagc cggatgaatc cttcatgata aaaacacaag gaaatgaggg
    13561 tgatcctgcg gcggtctgta atcgcattat aattgtctcg ctgaattcat ttgcgttcga
    13621 tgccacaccc ctgctggaga aaacaagatc cctatggacg aagttgagaa gctggaaaaa
    13681 gccattgctg gattagaaat tcaacgggcc attttggggg atgatgtggt caaggctgca
    13741 gtgagcgccc tgcgggagaa aatcagcctg ctgcaggcag agattcagcc agaacggcgc
    13801 aagcgaaaga tcgcgagtgt gttatttgca gatgtttcgg gttttacggc catgtctgag
    13861 cgcatggacc cggaagatgt cagcgacttg atgaaccgtt tgtgggagcg cattgaccag
    13921 gtgattattg atcacggtgg acggatcgat aaacatatcg gcgacgctgt gatggcgcta
    13981 tggggaaccg aggctgtccg agaagatgat ccggaacgtt ctgttcgagc ggcgctggct
    14041 gttcaaatag aactggctaa gataactcgc ggggaggggt ttttagatgc tcgcttcagg
    14101 ggagaagctc aagaactggc aatccgagta gcagtacata cgggaccggt gctgtggggt
    14161 caaaccggca ccactggcga atattctgcc attggggata cggtgaatac tgccagccgg
    14221 atgcagcacc aggcgccgct gggcggtgtg gtggtttcgg cagccacagc gctgcatatt
    14281 cagggattgt ttgatctgga agttctggag cccttgaaag ccaaggggaa gagtgaacct
    14341 gtccggatat tccgggtgct tcgccccaaa ccccgggctt acgccatgcg aacccgcggg
    14401 gtgatgggca tatcaacgcc gctggtgggg cgcgatgctg aactcaacga gatggtgaat
    14461 gtgctggagc attgcgttca aaatcagtcc tgctgtatgc ttaccatcac cggcgaagcc
    14521 ggaatcggta aatccagact gcagtatgag tttgaaaagt gtgtggaaca gtccgggcga
    14581 agatatcgga ttctgcgagg gcgagccggt caggatatgg taaatcagcc gtatgccctg
    14641 ctgcgagacc tgttttccaa tgcttttcag attcaagaca gtgacacgcg ggaagaagta
    14701 tggcaaaaat ttgaacaggg tattgcaacc ccatatcgcc tgcccgagca agataaaccc
    14761 ctacaggtga tgcaggcaca ttttatcggc caattgctgg gttttaattt caactccagc
    14821 gcgcatttgg ttggggcgat caaagacgcc aaacaattac acgaccgggc gctggtttac
    14881 ctgattgcat tcttccgtgc gatggctgaa caagaaccgg tgattatttt ccttgacgat
    14941 ttgcactggg ccgacgaaag ttcgctcgac catctcatcc acattttcag ggcgttgaac
    15001 catttgcgga ttctgtttgt ctgcatggct cgtcccactc tgtttgaacg ccgcccaggc
    15061 tggggaagcg gaaaaggcca tacgcagatt aagctgcagc cgctggcctt tgaagccagc
    15121 cagaaattgc ttgagcatat cttacaaaag cttgaggata tcccggagaa cctgagggat
    15181 atgattatcc aaaacgcgga ggggaacccc tactatattg aagaactggt caagatgctg
    15241 gttgaaaacg gggtgattat tcagactggt gagcagtggc gcgttgtgag cgaacgattg
    15301 caggttgaac aaatcccttc gaccctggcg gggattctac aggcccgttt ggacagcctg
    15361 acagagctgg aacgggatac gctgcagtgc tctgctgtgg ttgggcaaac cttctggaat
    15421 caggctgtgc agactttgaa cccggatgca tttgctgaac tggatgagac attagctgaa
    15481 ctcaatcaac gtgagctgat tgagccatca gaggtttcgg cttttgctga ttcaacggaa
    15541 tacgttttca ggcatcatat tttgtacggc gttacttatg acagcgttct caagagcctg
    15601 cggcgccaat atcacgctca gattgcccgc tggttgattg cccatgaggg cgagcgtgcg
    15661 ggagaattca gtggattgat tgccgaccat ctggaaaaag ccgagcgata cgatgaggct
    15721 gttgtttacc tgaggaaggc gggggagcgg gcaattcaga gttatgccaa caatgaaggg
    15781 gtgcagtatt ttagccgggc cattcacctg acggctgaag atgattacgc ggcccagtat
    15841 caattgcggt tggctcgtga aaatctgaat gatcttttgg gtgaacgggc ggcccaggca
    15901 caggaactgg atgcgcttga tgccctgaat gaccactttg acgccagcca gcaggctgag
    15961 attgccatcc ggcgcgcaaa cctgcacctg gtaaccggca attatgccat tggcctggaa
    16021 gctgccagaa aggccaccgc attagctgaa aagagcaaag acagccgaca cctggcaacg
    16081 gcattgatgc tgcaggcaca ggccctgtgg cgattgaatc agcgtccaga agcgcatgcg
    16141 ctgctgctgc gcgcgctgga actggcgagt acagtggagc aggcagagac aagatccgat
    16201 gtgttgagaa tgctgggaaa cctggcctat gatcaagata tttacgctga agccaggtca
    16261 tattttgaac aatcgcttga gatctgccgg cagattggtt ttcggcgtgg ggaatgcgcc
    16321 gttttgaatt ccctggggat tctgctgaac cggatcaatc ggggatctga tgaggcgctc
    16381 ggttgtta
//
LOCUS       DAOPVS010000032        16315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3626_length_16315_cov_55.287700, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000032 DAOPVS010000000
VERSION     DAOPVS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16315
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3626_length_16315_cov_55.287700"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3410
                     /locus_tag="PKL11_04590"
     CDS             <1..3410
                     /locus_tag="PKL11_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNZ12912.1"
                     /translation="GVYVENITVSKALTLEGPNAAINPNTGTRGAEAVILPTFSDPDP
                     TSATSSAIVYIAVDGVTIRGFSVDGDNPNLTSGVLVGTADVDAVEGIASYEGVGEIVI
                     ENNILQNYTYAAIDFYNYTDASATTDNYIRYNRLFNISSAAGYGIGVLIYNNFYADIT
                     DNVMTAVRVGVQTGNYYQANPGTTASISNNNIETTRRGIFHNLAYSDASPFTISGNTF
                     TVLDAPTGTKWDAIMLSSLQGSVNATVTGNIITGTDVTQLTAGYQIWNTPTTALLTIT
                     GGSVSGTDYGVWVNNFDGYNSDADNTAIILSGVQISDTTLAGVYILDNPSEVHGHTVK
                     ATLLDGVVINDCTTGIWVSGPQASVNSYGNLIQNNATGALFESDAAGTLANNSLTGNT
                     TAITNTGATLVNASSNWFGTADATAVAGLVSDLVDYTPWLNSGTDTGAAAGFQGDFSY
                     LNVDDDSPQAGSVGRIQEGVNLVSGSTVNVLPGVYAENVLVDKYVVITGAGSSDTADD
                     TIVTSPATVDYKVGVFQIAASGLSSAEPIVLQNMRVQPNGQAGVSIGRFTEATGVTVD
                     YLTLDNLWVIGTNNNAQTEQERGLYVDLTSTLNYLTVTDCAFNNLAYGWYIQKQVSAD
                     ASTVSNVVVTNTVFNHNNLKGIYTEKLTDATFTDCTIDQNGFDATGLPSYFIPWMSGV
                     DINLKAGTYQNIAFINATVTNNGLGGAKEGVGLAIKARTDGATYSPYPATLDNVLIAN
                     STITGNERGIRIGEPGKNNPGPTNVVIHHNRIFGNVQTYSGTDGTTYGGVVNQSAADV
                     TAENNWFGCNEGPNMAGCDATAAASTGLLDADPWLVLSAAADPTAVQPLGTSTIAADM
                     IFNSDAADTSAAGYLPDGIPAAYTAPDGGTLSETAALTLNGTLLPTTTFTAPAADQAY
                     QVCTAVDNELLCVDVTVQNVAPVAVDDTATTTEDTPVAILAVDLATNDTDANLDALTV
                     TAVSNPTNGTVMLEAGTITFTPTADFNGTAGFDYTVSDGVLTDTGHVTVTVTAVNDAP
                     VAVDNAYEMDEDTILNIEAPGVLNNDTDADGDTLTAMLVVGPTNGTLVLNADGSFTYT
                     PSPNFFGTDAFTYKASDGTSDSNVATVTITVVDMPETKYIYLPIILK"
     gene            3510..7253
                     /locus_tag="PKL11_04595"
     CDS             3510..7253
                     /locus_tag="PKL11_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ12913.1"
                     /translation="MPVGKPSVHAATSTPVSRPTVGVLLAEMGGWYGLPLLSGIEEAA
                     DRQDANIIYLVGASLEAAETGRTAIFRLARPERLNGLILHADLARHRSDEEIHQFCQQ
                     FAPLPLIGYALTTPEMPSVLADSYNGMHQLVTHLVKDHGYRKLAFIHGPAGQMEADER
                     FHAFQDVLLMHGLTINPEWVVRGDFSQESGRAAMEALLAHGRPQFEAVVAANDNMAVG
                     ALQVLHERGLRVPDDLALTGFDDTTESKLLSVPLTTVRQTFFASGQQSLISALRRIYG
                     ETLPNQILVPTSLVVRRSCGCVGEGMRQAAAQAVPGTTGSVQILKALTPQAVDHLVAA
                     AGERTDDDTVWGADARRFDMRTPMTETWNALLSDLESEKNHRFLAAFATALQQLQIVR
                     HDPMIWHEVVSALRNFIRPYLNDAQLVLRVENLLQQARLLVNETSLRSQSQRRGRIDR
                     LDQLLQKLTYSLATALNLDAVAETMAEHLPQMGIEALYLALYDVEGADPTPTTAHLAL
                     QFDHGKGAHLTEGESFPIIQILPPAIYPPQERSTFVVMPLTVGERQLGYMTVRVGPID
                     WEIYPRLRDLFSSTIFRIVLEEQQREAQNQMERLWADARLHAAELAQAKEQLEQAVTE
                     SNRRLKETEGLFEAIRSIVGANEIVDICQKLTRHFSRLVAADQTFIALVNHDERRVTL
                     EVSDGEIVENNDYETLVNGISGVVYRSGQPVLSLHAYDGIEPEASRQRRVQGGTGAVI
                     VAPIMVNQQVSGTVTAVRKLGQDPFAQHDVDLLTALAAQAGESIENALLFEAEREQRE
                     LAESLRQAGILVSSTLEFDQVLDQLLEQIQRVVDYDAGSLMLVDGDTVRVARGRGYVF
                     QSEAEKNRPTPEFSLESTITFKEMRNTGQPLVIPDTSSDPHWIKTARSISYRSWMGAP
                     IVSKKGIIGFFSLNKQQPNFFTPKMAQRLAAFANQAALALENASLYENMARFNLQLEE
                     RVRARTEELQNAYNVLEQMDRTKSDFISIASHELRTPITVLRGYSQILTTHPAIKSDE
                     SVQQMMDGILSGAQRMYEIVNSMIDLAKMDSRLMQLSLEQLPLGYIVRSEMQRFSSAV
                     IERKLTVTLNNLSDLPIIEADNESLRKVFTHLISNAIKYTPDGGTITITGKVLEPGEY
                     GLEEGVLISVADNGIGIDPQMRELIFASFFQTGEVSLHSSSKTKYKGGGPGLGLTIAR
                     GIVHAHHGQIWVESGGHDEDVCPGSIFYVALPVHQPKGQKEEA"
     gene            7446..8516
                     /locus_tag="PKL11_04600"
     CDS             7446..8516
                     /locus_tag="PKL11_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ12914.1"
                     /translation="MIIRLPGLIDPHVHVREPGATHKEDWESCTAAALAGGFTTILAM
                     PNTRPPVTDEASLRLALDSAQKARCDYAQYLGAGPDNAEAAARLAPGAAGLKLYLDQT
                     YGPLKLDDMTLWAPHFQHWPTAAPMAIHAEGRTLAAAIAFAGLYQRPVHLCHVAHREE
                     ILLIRKAKACGWPITCEVTPHHLFLSEVDLAEIGGRRGEVRPRLAALADQQALWDNLD
                     IIDCFATDHAPHTLAEKDSDTPPPGFPGLETALPLLLTAVKAGKLSLDDIIQRCYTNP
                     QRIFGLPVQADTWVEVDLEAEWTIHASELFSRCGWTPFEGQRVCGRVQRVMLRGRLAY
                     ENGRQLLPPGAGQNLRPELSSI"
     gene            8543..9526
                     /gene="pyrB"
                     /locus_tag="PKL11_04605"
     CDS             8543..9526
                     /gene="pyrB"
                     /locus_tag="PKL11_04605"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNZ12915.1"
                     /translation="MATHLPPLKRHNPHLPFGDHQNAPYYGSDIISVKQFERQDLEYV
                     FAVAHEMQEMVMRVGTFDLLKGKILASLFYEPSTRTSSSFTAAMERLGGSVIPINEVR
                     YSSVSKGESLPDTVRTLEAYADVIVLRHPEVGSAALAAKYARKPVINAGDGIGEHPTQ
                     ALLDLFTIQAEMGKIDGLTITLLGDLKHGRTVHSLARLLSLYDVKINYVSPEILRMPA
                     EIMEEVSRPQAEYRELDPVLANSDVLYVTRVQKERFDDPAIYEAVKGTYTITLETMTR
                     AKEDMIVMHPLPRVGEISEEVDDDSRAAYFRQMEYGLYTRMALLAMVLGKA"
     gene            9526..10950
                     /gene="pyrF"
                     /locus_tag="PKL11_04610"
     CDS             9526..10950
                     /gene="pyrF"
                     /locus_tag="PKL11_04610"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNZ12916.1"
                     /translation="MQTPVGFFTRLDQRARETGSLLCIGLDPHPGDLATPTAAGARDF
                     CLRLIEATQEVALAYKPNAAFFEAFGAEGWQALQDVIATIPEGIPVILDAKRGDIAST
                     AEAYARSAFETLGAQAITLNPYLGYDSIEPFIKAPEHGVFLLCKTSNQGAGDLQDLRL
                     IGTRQARHLYEQVAVLAQKWNDRQNVGVVVGATHPQALADVRKLAPDLWILAPGVGAQ
                     GGDLHAALTAGLRADGLGLLIPVSRAISRAADPRAAAQALFKDIQQARQTLRREALGE
                     QIPPALAEGLLQAGCVKFGSFTLKSGLVSPIYIDLRQLASHPALLAEVGAAFIPLLEK
                     LHFDRLAALPYAAMPIGTAISLQSGWPMIYPRKEAKTYGTRAEIEGQFAPGETAVVID
                     DLATTGGSKFEAIEQLTGAGLVVKDVVVLIDRQSGAREALEKEGYQLHAVLTLTGLLD
                     TWERSGRVPTEQIEAVRQFLKENG"
     gene            10951..11994
                     /locus_tag="PKL11_04615"
     CDS             10951..11994
                     /locus_tag="PKL11_04615"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNZ12917.1"
                     /translation="MDLYQLVKPLIYKLQPEQAHHLTIAMLQMGGGIAPGRWLIRAMF
                     HARQNGPEVKAFGLTFPNPLGMAAGYDKDGLGWRGLAGLGFGHIEVGTVTPKAQPGNP
                     TPRCFRLVPDEAVINRMGFPNRGADFLARRLRFKRPKGLILGVNIGKNKVTPLEEAAN
                     DYVMLVEQFATLADYLAVNVSSPNTPDLRKLQAGDALTPLLSAIQAAREAQTAKLGRK
                     TPVLVKLAPDLSETELDAALDAIVNTRMDGVIIGNTTISRPTLQSPMAGEIGGLSGKP
                     LSAINQAMVGRVVKLLDGKLPVVASGGVMTPADAQARLDAGATLVQLYSGLIYSGPAL
                     VQQILNAGLKSSH"
     gene            complement(12064..12264)
                     /locus_tag="PKL11_04620"
     CDS             complement(12064..12264)
                     /locus_tag="PKL11_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12918.1"
                     /translation="MKSPAYYSFMLRIWSSTEASCQVWRASLEDPLTRQVMGFGSIEA
                     LTEYLKQQTTAPPNSQDRSDFE"
     gene            complement(12298..13845)
                     /locus_tag="PKL11_04625"
     CDS             complement(12298..13845)
                     /locus_tag="PKL11_04625"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNZ12919.1"
                     /translation="MKIKPLIFVLVLSLALTALAVPTQPVRAAETFVVDTQNDTNDAN
                     PGNCVCADSGGNCSLPAAIQEANACPGADTIRLSHVAYQLEDRVFEITEELYIYNPFD
                     TFITISNGSVFRLFVVNTGVKLTLDTVKINYSGGIESNGQLWLYDCSLEDNQPDTGED
                     GGAIWSGSSSGYLYIDHSWITNNDAGTGGDGGGIYSNSPTVIFDAFITGNNAVNGGGI
                     YLAGGSQSLIYESKISTNTATGSGGGVYVVAGGYGASHSISGSHIAENDAQNGGGLFA
                     TDTDIYLTNTSLIANHATSNGGGLLISTQGTYPQAYLINVTVGANAAENHGGGLYFGA
                     DTRGDLSNVTITQNTADWSITGVGDGSGDGGGIASASQYSVVLRNSLVAENSDRSLVA
                     PPAYGDPDCDGSFESDGYNLIGNKTCTITAQTGDHFGNRFSPVDPLLGDRVDNATPNY
                     TSYYPILESSPLIDRGNPSGCVNSDDELITEDQLERARPQGMHCDIGAYESLFSLTGI
                     YLPLVLR"
     gene            13965..>16315
                     /locus_tag="PKL11_04630"
     CDS             13965..>16315
                     /locus_tag="PKL11_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNZ12920.1"
                     /translation="MKLTVQLFGVLEVKLDGQPVTGLRTDKVRALLTYLLIERAHPHP
                     REALAELLWPDQAPEAALHNLRQALSTLRKALGGDGTGELGTPFLLFQEDMVAWNPAA
                     ETWVDVLAFTEACAAAQQFHKRNAGLAGLNLRRLRRALSYYRGPFLAHFSLGDSSGFE
                     EWAMLTREALNRQAVEAMDALVEYHERRGEYSQAREMATRLLELAPWDETAHGEVMRL
                     LALEGQWSAAQAQYAACRRYLKNELGLEPSAETQALFERIRQGAGQNRPPEPRYPTGQ
                     HNLPGGLTPFTGRQNELDELADLLAKPDCRLITLLGPGGIGKTRLALEAAREQAGLHT
                     RGVFFIPLSGVSHCEQIPAAIAEGLGLPGSEREDPHTRLIQALSGQTILLTLDNLEHL
                     TDGIDCLSALLAAAPGVKILATSRTRLNLQEEWVYRVDGLAYPDGTPRQPLEDYSAIQ
                     LFMQTLERTQQRAALTPVDFEALIEICQLVEGSPLSLELAASAHRARGLGEIAAELRQ
                     NIDVLATSLVNVPARHRSLRATLNYSWMLLDPEEQIGLARLSVFRGGFETEAGQAVAE
                     ILPDVLARLHDHLLLRRDERGRWGLHESVRQYAAEKRGMDEQSDREVCNRHSRFFAEY
                     VHQRTGQLETLDQGEALNAIAANHENIVAGWENAIANRQLARLDQYLRGLYLFYDFRS
                     RYHDGAALLGRALKQLAGEPQAAPLYGRMLARLGIMQSRLSQNDEALVTLTDALHQLA
                     DTDQPDEQAICMVWLANLIRKRKSQAEAGELARQALEISRKID"
BASE COUNT         3687 a         4341 c         4955 g         3332 t
ORIGIN      
        1 ccggcgtcta tgttgagaac atcacggtca gcaaagcgct gaccctggaa ggtccgaacg
       61 cggcgatcaa ccccaacacg ggcacacgcg gcgcggaagc agtgatcctt ccaacgttct
      121 cggatcctga tccaaccagc gcgacatcgt ctgcgatcgt ttatatcgcg gttgatggtg
      181 tgaccattcg cgggttctcg gtggatgggg acaaccccaa cctgaccagc ggggtgctgg
      241 ttggcactgc ggatgttgat gctgttgaag gcatcgccag ttatgaaggt gtgggcgaaa
      301 tcgttattga aaataacatt ttgcagaatt acacctatgc tgcgatcgac ttctataact
      361 ataccgacgc cagcgcgacg accgacaact atatccgtta caaccggctt ttcaatatct
      421 catcagcggc cggatacggg attggcgtcc tgatttacaa caacttctat gctgacatca
      481 ccgacaacgt catgacggca gtgcgcgtgg gtgtgcaaac cggtaattac taccaggcca
      541 acccgggcac cacggcctca atcagtaata acaacatcga gaccactcgc cggggtatct
      601 tccacaacct ggcgtacagc gatgcctccc ccttcaccat cagcggcaac acattcactg
      661 tgcttgacgc gccaaccggc accaagtggg acgccatcat gctctcctca ctgcagggca
      721 gcgtcaatgc cacggtcacc ggcaacatca tcaccggcac cgatgtcacc cagctgactg
      781 ccggttacca gatctggaac accccaacaa cggccctgct gaccatcact ggcggcagtg
      841 tcagcggcac ggattacggc gtctgggtca acaactttga tggctacaat tcggatgccg
      901 acaataccgc aatcatcctg agcggggtgc agatttcaga taccaccctg gcgggtgtct
      961 acatcctgga caacccgtcc gaagttcatg ggcacacggt caaagcaacc ctgctggacg
     1021 gcgtggtcat caatgactgc accaccggca tctgggtttc cggaccccag gcgagcgtga
     1081 actcctatgg gaacctgatc cagaacaatg ccaccggggc gctgtttgaa agcgatgcgg
     1141 caggaacgct tgccaacaac agcctgaccg gcaacaccac cgccataacc aataccggtg
     1201 caaccctggt caatgcctcc agcaactggt tcggcaccgc cgatgccacg gctgtggctg
     1261 gattggtcag cgacctggtc gactacactc cctggctaaa ctccggcacc gataccggcg
     1321 ctgcggccgg cttccaaggc gacttctctt acctgaatgt tgacgatgac agcccgcaag
     1381 ccgggtctgt gggtcgcatt caggaaggtg tcaaccttgt ttccggcagt acggtcaatg
     1441 tccttccagg cgtctatgcc gaaaatgtgc tggtggacaa atatgttgtc atcaccggcg
     1501 ctggcagcag cgatacggct gacgatacga ttgtgacgag cccggcaacc gtggactata
     1561 aagtgggtgt attccagatt gccgcatccg ggttatcatc tgctgagccc attgtgctgc
     1621 aaaatatgcg cgtccagccc aatgggcagg cgggcgtcag tattggacgt ttcaccgagg
     1681 ctactggtgt gacggtggac tacctcacac tggacaacct ctgggtaatt gggaccaata
     1741 acaatgccca aaccgagcag gaacgcggtt tgtatgtgga cctgacttca acgctcaact
     1801 atctgacggt aacagactgc gctttcaaca atctggccta tggctggtat atccaaaagc
     1861 aggttagtgc ggatgccagt acggtcagca atgtggtggt taccaatacc gtctttaatc
     1921 acaacaatct taagggtatc tataccgaaa agttgacgga tgcgaccttt acagattgca
     1981 ccatcgatca aaacggtttt gatgccactg gtttaccgtc ctacttcatc ccgtggatga
     2041 gcggcgtgga tatcaacctc aaggccggta cgtatcagaa cattgccttc atcaacgcaa
     2101 cagtaaccaa caatggcctg ggcggcgcca aagaaggcgt aggtttggcc ataaaagccc
     2161 gcaccgacgg tgccacatac agcccttatc cggcgacact ggataacgtg ttgattgcca
     2221 acagcactat caccgggaac gagcgtggca tccggattgg cgaaccaggc aagaataatc
     2281 ccgggccaac caatgttgtc atccaccata accgcatctt tgggaacgtc caaacctaca
     2341 gcggcacaga tggaacaact tatggcggtg tggtcaacca atctgccgca gatgtaacgg
     2401 ctgaaaacaa ctggttcggc tgcaacgaag ggcccaatat ggcgggctgt gatgcaacgg
     2461 ctgcagcaag cacaggattg ttagacgccg acccatggct ggtgctttcg gctgccgcgg
     2521 atccgactgc agtacaaccc ctgggtacct caaccatcgc ggccgatatg atcttcaact
     2581 cggatgccgc agatacctct gccgctggct acctgcctga tggcatcccg gcagcttaca
     2641 ctgccccgga tggcggcaca ctatctgaaa ccgcagccct tactctgaat ggcaccctgc
     2701 tacccacaac cactttcaca gccccggctg cagatcaagc ttaccaggtt tgcaccgcag
     2761 tagataatga actgctctgt gtcgatgtga ctgttcaaaa cgtagcccct gtggccgtgg
     2821 atgacacagc cacaacgact gaagacacgc cggtcgctat cctggcagtt gacctggcta
     2881 ccaacgatac cgatgccaat ctggatgctc tcactgtaac tgcggtcagc aacccgacca
     2941 acggcaccgt gatgctggaa gcgggcacga ttaccttcac ccccaccgcc gacttcaacg
     3001 gtaccgccgg gtttgactac accgtcagcg acggtgttct gaccgacacc ggtcacgtca
     3061 ctgtcacggt gactgctgtc aacgacgccc cggtggcggt ggacaatgcc tacgagatgg
     3121 atgaggacac catcctgaac atcgaagctc cgggtgtgct gaacaacgac accgatgccg
     3181 atggcgatac cctcactgcc atgctggtgg tgggtccgac caacggcacc ctggtgctca
     3241 acgccgacgg ttcgttcacc tacaccccgt cgcccaactt cttcggcacc gacgccttca
     3301 cctacaaggc cagtgatggc actagcgact ccaacgtcgc caccgtcacc atcaccgtgg
     3361 tggatatgcc cgagacgaag tacatctacc tgccgattat cctcaagtaa gccgagcgat
     3421 agcggattga ccattcgggc gggtgcatta gcacccgccc gaaatttttt tatgatagaa
     3481 tacattaagt ctgcagtgga ggtattgctg tgccggttgg aaagccatca gtccacgccg
     3541 caacatcaac cccggtctca cggccaacgg ttggtgtttt gctggctgag atgggcgggt
     3601 ggtatgggct gcctttactg tcggggattg aggaagccgc tgaccggcaa gatgcgaata
     3661 tcatttatct ggtgggggcc agcctggaag ctgctgaaac cgggcggact gctatttttc
     3721 gattggcgcg gccggaacga ttaaatggcc tgattttaca tgccgacctg gcgcggcacc
     3781 gctcagatga ggagattcat cagttctgcc agcagtttgc gccactgcca ttgattggat
     3841 atgcattgac cacgcctgaa atgccttctg tgctggcaga cagctacaac ggtatgcacc
     3901 aactggtgac gcatctggtc aaagaccacg gataccgcaa gctggccttc atccatggcc
     3961 cagccgggca aatggaagcg gatgaacgct tccatgcgtt tcaggatgtg ctgctcatgc
     4021 atggattgac catcaacccg gaatgggtgg tgcggggaga tttttctcag gagagcggac
     4081 gggcagccat ggaagcgctg ctggctcacg ggcggccgca atttgaggct gtggttgccg
     4141 ccaacgataa tatggcggtg ggggcgctgc aggtgctgca tgagcgcggc ctgcgggtgc
     4201 cggatgacct ggccctgact ggatttgatg atacaaccga atcaaagctg ctcagtgtgc
     4261 cgttaaccac agtgcggcaa accttctttg ccagcgggca gcaaagcctg atttctgctc
     4321 tgcgacgcat ctatggagaa acactgccaa atcaaatctt agtgccgacc agcctggtgg
     4381 tccgccggtc gtgcggctgt gtgggtgaag ggatgcggca ggctgcggca caagctgtgc
     4441 caggaaccac gggttcggta cagattctaa aggcgttgac accccaggca gtggatcacc
     4501 tggtggcggc tgctggagag cgcaccgatg acgataccgt ctggggggca gatgcacgcc
     4561 ggtttgacat gcgtacgcca atgacagaaa cctggaacgc attgttgagc gatctggaaa
     4621 gcgagaaaaa tcaccgcttc ctggcagcat ttgcgactgc gctgcagcaa ctgcaaatcg
     4681 tccggcatga ccccatgatc tggcacgagg tcgtctcggc gctgcggaat tttatccgac
     4741 cttatttgaa tgatgcgcag cttgtgctgc gggttgagaa tttgctgcag caggcgcgcc
     4801 tgctggtgaa cgaaacttca ttgcgatcac agagccaacg gcgcgggcga attgatcggc
     4861 tagaccagct tttacagaag ctgacttaca gcctggctac ggcgcttaat ctggacgcgg
     4921 ttgccgaaac gatggctgag catctgcccc agatggggat tgaggcgtta tatctggcgc
     4981 tttatgatgt ggaaggggca gacccgaccc ccactacggc acacctggcg cttcaatttg
     5041 accacggcaa gggggcgcac cttaccgaag gtgagagctt cccaattatc cagatactgc
     5101 ccccggcaat atacccgccc caggaacgct cgacctttgt ggtgatgcca ttgacggttg
     5161 gcgagcgcca gttggggtac atgaccgtcc gcgtggggcc gattgactgg gagatttacc
     5221 cccggctgcg cgatctgttc agcagcacga tctttcggat tgtgctggaa gaacagcagc
     5281 gggaagcgca gaatcagatg gagcggttgt gggccgacgc ccgactgcat gcggcagaac
     5341 tggcacaggc taaagagcag ttggaacagg cagtcaccga atccaaccgc aggttgaagg
     5401 aaactgaggg gttgtttgag gctatccgct cgattgtggg ggcgaatgag attgtggata
     5461 tttgccagaa gctgacgcgg catttctcgc ggctggtggc agccgaccag acctttattg
     5521 cgctggtcaa tcatgatgag cgccgggtga cgctggaagt gagcgacggc gaaattgttg
     5581 aaaacaacga ttacgaaacc ctggtgaatg gaatcagcgg ggtagtgtac cgcagcgggc
     5641 agccggtctt gtcgctgcac gcgtatgatg ggattgagcc ggaagcctcg cggcagcgcc
     5701 gcgttcaggg tggcacgggg gcggtgattg ttgcaccgat tatggtgaat cagcaagtca
     5761 gcggaactgt gactgccgtg cgaaagctgg gtcaggatcc ctttgcgcag catgatgttg
     5821 atctgttaac cgctttggcc gcccaggctg gtgaatctat tgaaaatgcg ctgttgtttg
     5881 aggctgagcg cgagcagcgc gagctggctg aatcgctgcg gcaggcgggg attctggtgt
     5941 catccacgct ggaatttgac caggtgttgg accaactgtt agagcagatc caacgggtgg
     6001 tggattatga cgctggcagc ctgatgctgg ttgatgggga taccgtgcga gtggctcgcg
     6061 ggcgcgggta tgtgtttcag agtgaggcgg agaaaaaccg cccgactccg gagttttcac
     6121 tggagagtac aattacattt aaagagatgc ggaataccgg ccagcccctg gtgattccag
     6181 ataccagcag cgatccccac tggattaaaa ccgcccggtc gatcagttac cgatcctgga
     6241 tgggggcgcc gattgtttcc aaaaaaggga tcatcgggtt cttctcgctg aacaagcagc
     6301 agcccaattt ctttacaccc aagatggcgc agcggctggc ggcttttgcc aatcaggcgg
     6361 ccctggcgct ggaaaatgcc agcctttacg aaaacatggc ccgtttcaac ctgcaattgg
     6421 aggaacgggt gcgcgcccgc accgaggaac ttcagaatgc ctataatgtg ctggagcaga
     6481 tggatcgcac caagagtgat ttcatcagca ttgcttcgca tgagctgagg acgcccatca
     6541 cggtgctgcg cgggtacagc cagattttga ccacccaccc ggcgatcaaa agcgatgaat
     6601 ctgtccagca gatgatggat gggattctct ccggggcgca aaggatgtat gagattgtca
     6661 acagcatgat tgacctggcc aagatggaca gccggttaat gcagttgagc ctggagcagc
     6721 tgccgctggg ctatattgtg cgcagcgaga tgcagcggtt tagcagcgca gtaattgaac
     6781 gcaaattgac ggtgaccctt aacaacctga gcgatctgcc gattatagaa gcagacaatg
     6841 aatcgctgcg aaaagtgttt acacacttaa tcagtaatgc catcaaatat acgcctgacg
     6901 gcggcacaat caccatcaca gggaaggtgc tggagcccgg tgagtacggg ctggaggaag
     6961 gggtactgat cagcgtggca gataacggga ttgggattga cccccagatg cgggagctga
     7021 tctttgccag tttcttccag acaggtgaag tatccttaca ttcaagcagt aagacgaagt
     7081 ataagggcgg cgggcctgga ttgggtttga caatcgcgcg cgggatcgtc catgcgcacc
     7141 acgggcaaat ctgggtcgaa tcgggtgggc acgatgaaga tgtctgcccg ggcagcatct
     7201 tctatgtagc attgccggtc catcaaccca aggggcaaaa agaagaggcc tagatcaaca
     7261 agattgtcca aacaattggg caatactctt gacagaattg ccatcttgcg tagaatcaac
     7321 ccgatgcatg taccgggacc actcaacaaa atgaaaccca ccctgttgcc agggcaacgt
     7381 tctgatttat ccattcaaga gagccattat tggctctctt tttttgcctg tttacggagg
     7441 gatgcatgat cattcgcctg cctggactga ttgatccgca tgtgcatgtg cgtgaacccg
     7501 gtgccaccca taaagaagac tgggagagct gcacggctgc ggccctagcg ggaggcttta
     7561 caaccattct ggcaatgccc aatacccgcc caccagtgac cgatgaagcg agcctgcggc
     7621 tggcactgga cagcgcccag aaggcccgct gtgactatgc gcaatatctc ggtgcgggac
     7681 ctgacaatgc agaagcggct gcccggctgg cgcccggcgc ggcgggattg aagctttatc
     7741 tggaccaaac ttatgggccc ttgaaactgg acgacatgac cctgtgggcg ccacactttc
     7801 aacactggcc aactgcggcg ccgatggcta tccatgcaga aggccgcacg ctggcggcgg
     7861 caattgcttt tgcaggtcta taccaacgtc ccgtccatct gtgccacgtg gctcaccgtg
     7921 aagaaatttt gctgatccgc aaggccaaag cctgcggctg gcccatcacc tgtgaagtga
     7981 cgccgcacca cctattttta tcagaggttg atctggcaga aattggaggt agacgcggcg
     8041 aggtgcgccc gcggctggct gcactggccg accagcaggc gctgtgggac aacctggata
     8101 ttattgattg ttttgccacc gaccatgcgc ctcacacgct ggcagaaaaa gacagcgata
     8161 ccccaccgcc agggtttccg gggttggaaa cagccctgcc gctgttgtta acggcggtaa
     8221 aggcgggcaa actcagcctg gatgatatta tccagcgctg ctacaccaac ccccaacgaa
     8281 tatttgggct gccggtacag gcagatacct gggttgaggt ggatttggag gctgaatgga
     8341 ccattcacgc gagcgaactg ttcagccgct gcgggtggac cccgtttgaa ggccagcggg
     8401 tttgcgggcg cgttcaacgg gtgatgctgc gcggtagact tgcctatgaa aatggccggc
     8461 agttactgcc gccgggcgcc gggcaaaatt tacgcccaga gctatcgtca atttgagatt
     8521 aatcgaaaaa aggagaacaa ctgtggctac tcatcttccc ccactcaaac ggcataatcc
     8581 acatttgccg tttggtgatc atcaaaatgc gccgtattat ggcagcgata tcatttccgt
     8641 caaacagttt gaacggcaag acctggaata tgtctttgct gtggcccacg aaatgcagga
     8701 aatggtgatg cgggtgggta catttgatct actgaaaggc aagattttag ccagtttatt
     8761 ctatgagccc tccacccgca catcgtcttc gttcacagcg gcgatggaac ggctgggggg
     8821 gagtgttatt ccaatcaacg aggtgcggta ttcgtcggtt tcaaaaggcg agtcgctgcc
     8881 agacacagtg cgcacgctgg aagcctacgc cgatgtgatt gtgctgcggc acccagaagt
     8941 gggttcggcg gcgctggcgg caaaatatgc ccgaaagccc gtgatcaatg ccggcgatgg
     9001 aattggggaa caccccaccc aggcgctgct ggatctgttt accatccagg cggaaatggg
     9061 aaaaattgac gggctgacga ttacgctgct gggtgatttg aagcacgggc gcacggtcca
     9121 ttcgctggcg cggctgcttt cgttgtatga tgtgaagatc aactatgtgt cgccggagat
     9181 tttgcggatg ccggcggaga tcatggaaga ggtttcgcgg cctcaggcag agtatcggga
     9241 attggacccg gtactggcga atagcgacgt cttatatgtg acccgagtgc agaaggagcg
     9301 atttgacgac ccggcgattt atgaagctgt caaggggacg tacaccatca cgctggaaac
     9361 catgacgcgc gcaaaggaag atatgattgt gatgcacccc ctgccgaggg tgggtgaaat
     9421 cagcgaagag gtggacgacg actcgcgggc ggcatacttc cggcagatgg aatacggttt
     9481 gtacacccgc atggcactgc tggcgatggt tctggggaaa gcctgatgca aacaccagtg
     9541 ggtttcttca ccagattaga tcaacgcgcc cgtgaaacgg gatcgctgct gtgcattggg
     9601 ctggatcctc atcccggcga tttagcgaca cccacggcag ccggggcgcg cgatttttgc
     9661 ctgcggctga ttgaagccac acaggaggtt gccctggcct acaaacccaa cgcggccttt
     9721 tttgaggcct ttggcgctga aggctggcag gctctgcaag acgtgattgc aaccatcccg
     9781 gagggcattc ctgtgatttt ggatgccaaa cgcggagata ttgccagcac ggcggaagcc
     9841 tatgcccgct cagcttttga gacgctgggc gcccaggcca tcacgctcaa cccctacctg
     9901 ggatatgact cgattgagcc gtttatcaag gcgccggagc atggcgtttt tctgttgtgc
     9961 aagacctcga accaaggcgc tggggacctg caggacctgc gcctgatagg aacgcggcag
    10021 gcacgacatc tgtacgagca ggtggctgtg ctggctcaaa agtggaatga ccgtcagaat
    10081 gtgggggttg ttgtgggagc tacacaccca caggcgctgg cagatgttcg taaactggcc
    10141 cccgaccttt ggattctggc gccaggcgta ggcgcccagg gcggcgacct gcatgcggca
    10201 ctgaccgccg ggctgcgcgc cgatgggttg ggattgctga tccccgtttc gcgagcaatc
    10261 tcacgagcgg cagaccccag agcagccgca caggccctgt tcaaagatat tcaacaggcg
    10321 cgacaaaccc tgaggcgcga agcattgggc gagcagattc cgccagcgct ggcggagggg
    10381 ctgctccagg cgggatgcgt caagtttggc agctttacgc tgaagtccgg gttggtttcg
    10441 ccgatttaca ttgacctgcg gcagttggca tcgcatccgg cactgctggc cgaagtcggc
    10501 gcagccttca tcccgctgct ggaaaaactg cactttgacc gattggcggc actgccctat
    10561 gctgccatgc ccatcggtac agccatcagc ctgcaaagcg ggtggccgat gatttacccg
    10621 cgcaaagagg ccaaaaccta tgggacgcgg gcggaaattg agggtcaatt tgcgccggga
    10681 gaaaccgcgg tggtgattga cgacctggca acgacaggtg gcagcaaatt tgaggccatt
    10741 gagcaattga cgggcgccgg gctggtggtg aaggatgtgg tggtgttgat tgaccggcag
    10801 tcgggggcaa gagaagcgct tgagaaggaa gggtatcagc tccatgctgt attgacgctg
    10861 accgggctgc tggatacctg ggagcgcagc gggcgcgtcc cgacagaaca aattgaggca
    10921 gtacgtcagt ttttgaagga gaatggatag atggatttgt atcaactggt caagcccctg
    10981 atttacaaat tgcagccgga gcaggcgcac catctgacga ttgccatgct gcaaatgggc
    11041 ggcgggattg cgcccggccg ctggctcatc cgcgcgatgt tccacgcccg gcaaaatggg
    11101 ccggaggtga aggcctttgg gttaactttt cccaatccgc tgggcatggc tgccgggtat
    11161 gataaagatg ggctgggctg gcgcgggttg gcagggctgg ggttcggaca tattgaggtc
    11221 gggacggtga cgcccaaagc ccagccggga aaccccacgc cgcgctgctt ccgactggtg
    11281 cccgatgaag cggtgatcaa tcggatggga tttcccaatc ggggagcgga ttttctggcc
    11341 cggcggttga ggttcaagcg acccaagggg ttgatcctgg gcgtgaatat tggcaagaac
    11401 aaagtcacgc cactggaaga agcggcaaac gactacgtga tgctggtgga acaatttgcg
    11461 accctggcag attatctggc ggtgaacgtc agttcgccaa atacgccgga tttgcgcaag
    11521 ctgcaagccg gagatgccct gacgccgctg ctgagcgcca ttcaggcagc gcgggaagcg
    11581 caaacagcca aactggggcg taaaaccccg gtgctggtca agctggcacc cgatctgagc
    11641 gagacggaac tggacgcggc gctggatgcc atcgtcaata cccggatgga tggggtaatt
    11701 atcggtaata ccaccatcag ccggcctaca ctgcaatcac caatggcagg tgaaattggc
    11761 gggttgagcg gcaagccgct gagcgccatc aatcaagcga tggtggggcg ggttgtgaag
    11821 ctgctggatg gcaagctgcc tgtggtggca agcggcgggg taatgacccc tgcggatgct
    11881 caggcccggc tggatgccgg agcaacgctg gtgcagctct attcggggct gatttactca
    11941 ggaccggcgc tggtacaaca gatactcaat gcggggctga agagcagcca ttaatcgcat
    12001 aaaaaacagg cataagatgc aggcaaaatc tcgcccgggc aggtggcccg ggcgaggggg
    12061 gaattactca aagtcggatc tgtcttgtga attgggtggg gcagtggttt gctgcttgag
    12121 gtattcggtc aaagcttcaa tactgccgaa acccatcacc tgccgggtca gtggatcttc
    12181 cagagaggcg cgccagacct gacaactggc ctcggtactg gaccaaatgc ggagcataaa
    12241 ggagtagtag gctggtgatt tcacaagcat ccccttgacc ggatgggtta gtggtaacta
    12301 gcgcagcacg agcgggagat agatgccggt gaggctgaac aatgattcgt aggcaccaat
    12361 gtcgcagtgc atgccctgag ggcgtgcgcg ttccaattga tcttcagtga taagctcatc
    12421 atcagaattg acgcatccgg agggattgcc ccggtcaatg agcgggctgg attccaggat
    12481 ggggtagtat gaagtgtagt tgggggtggc attatcaaca cggtcgccca gcaaggggtc
    12541 aacgggcgaa aagcggttgc cgaaatgatc gccagtttgc gccgtaatgg tgcaggtttt
    12601 gttgccaatc agattgtagc catctgactc aaatgagcca tcacagtcgg gatcgccgta
    12661 agcgggcgga gcaaccagcg agcgatcgct gttttctgcc acaaggctgt tgcgcagcac
    12721 aactgaatac tggctggcgc ttgcaatgcc gccaccatca ccgctgccat cgcctacacc
    12781 ggtaatgctc cagtcggcgg tgttctgggt tatggtcaca ttggataagt caccgcgggt
    12841 gtcggcaccg aaatagaggc cgccgccgtg gttttcagcg gcattggcgc ccacggtgac
    12901 attgatcaga taggcctgag gataggtgcc ctgggtgctg attaacaggc caccgccgtt
    12961 actggtggcg tggttagcga tcaggctggt gttggtcagg taaatatctg tatctgtggc
    13021 gaaaagaccg ccgccgtttt gggcatcatt ttcagcgatg tggcttccgg agatggaatg
    13081 agaggcgccg taccccccgg caaccacata cacgccgccc ccgctgccgg tggcggtgtt
    13141 cgtgctgatt ttggattcgt agatcaaaga ctgagagccg cccgccagat aaatgccgcc
    13201 gccgttgacg gcattattcc ctgtgatgaa ggcgtcaaaa atcacggttg gagagttgct
    13261 ataaatacca ccgccatcgc cgccggtgcc agcatcgttg ttggtaatcc aggagtggtc
    13321 aatatagagg tacccgctgg aagagccgga ccaaattgcg ccgccatctt caccagtatc
    13381 gggctggtta tcttccagcg agcaatcgta gagccagagc tgtccgttgc tctcgatgcc
    13441 gccagagtag tttattttga ccgtatccag ggtgagcttt acgccggtat tgactacaaa
    13501 gagacgaaag actgagccat tggaaatcgt gataaaagta tcaaagggat tgtaaatgta
    13561 caactcctca gtgatttcga agacgcggtc ttcaagctga taggccacat gagagaggcg
    13621 aatggtatcg gcgcccgggc aggcattggc ctcttgaatg gcggcaggca aactgcagtt
    13681 gccgccgctg tcggcacaga cgcagttgcc gggattggca tcattggtat cattttgggt
    13741 atctactaca aacgtttctg cagcgcggac cggctgggtg ggcacagcca gggctgtaag
    13801 cgcgagcgat aagacaagga caaagattaa gggtttgatt ttcatggttc ttctcctgaa
    13861 taaaatacaa ggaatagcaa gacgctgatt acaggatacc cattgaccgt caacaaatcg
    13921 tcaaaaattc agcatcaggt ataattattc aaggttccca cactgtgaaa cttacggttc
    13981 agcttttcgg ggtattggaa gtcaaactgg atggtcagcc tgttacaggg ctgcggactg
    14041 acaaagttcg agcactgctg acctacctgt taattgagcg cgcccatccc cacccccgcg
    14101 aggcgctggc agaactgctg tggccggatc aagcgccgga ggcagcgctg cacaacctgc
    14161 ggcaggcgct ttccaccctg cggaaggccc tgggcgggga tggcaccggt gaactgggaa
    14221 cgcctttctt attatttcag gaggatatgg ttgcctggaa cccggcggca gagacctggg
    14281 tagatgtgct ggcttttaca gaggcgtgcg cggcggcgca gcagtttcac aagcgaaatg
    14341 ctggtctggc gggtctcaat ttgcgccggc tgcgccgggc cttaagttat taccgcgggc
    14401 cattcctggc gcatttttca ctgggagaca gctctggttt tgaggaatgg gccatgctga
    14461 cgcgcgaggc gctgaaccgg caggccgtgg aagcaatgga tgcactggtg gaatatcatg
    14521 aacgccgggg ggaatattca caggcacggg agatggctac ccgactgctt gaactggcac
    14581 cctgggatga gaccgcccac ggtgaggtga tgcggctgct ggcactcgaa ggacaatgga
    14641 gcgcggcaca ggcgcagtat gcagcctgcc ggcgatattt gaaaaacgag ctgggactgg
    14701 aaccgtcggc ggaaacccag gcattgtttg agagaatccg gcagggggca gggcagaaca
    14761 ggccgccgga accacgctac cccaccggac agcacaacct gccgggagga ttgacgccgt
    14821 ttaccgggcg acaaaatgaa ctggatgaac tggccgacct gctggcaaag ccggactgcc
    14881 gcctgatcac gctgttggga ccgggtggga ttgggaaaac ccggctggcg ctggaggcag
    14941 cccgcgaaca ggcggggttg catacccggg gtgtattctt cattccgctc agcggggtca
    15001 gccactgcga acagattccg gcagcaattg ctgaggggtt ggggctgccc ggctctgagc
    15061 gtgaagatcc ccacacgcgg ttaatccagg ccttgagcgg gcaaactatt ttgctgacgc
    15121 tggataacct ggagcatctg accgacggga ttgactgcct gagtgcacta ctggcagccg
    15181 cgcccggcgt gaaaattctg gcaacttcca gaacacgctt gaatttacag gaagaatggg
    15241 tctatcgggt ggatggattg gcgtacccgg atgggacacc ccggcagccc cttgaagatt
    15301 acagtgcaat ccagttgttt atgcagaccc tggagcgcac ccagcagcgt gcggcgctga
    15361 ccccggtaga cttcgaggcg ttaattgaaa tttgccagct ggttgaaggg tcgccgctga
    15421 gtctggaact ggcggcctcg gcccatcggg cacgcggact gggggagatt gcggcagagc
    15481 tccggcaaaa tattgatgtg ctggcgacga gcctggtgaa tgtgccggca cggcaccgca
    15541 gcctgcgggc tacgttgaac tattcctgga tgctgctgga cccggaagaa caaatcgggc
    15601 tggcccggtt gagcgtgttt cgcggcgggt ttgaaacaga agccgggcag gctgtggcgg
    15661 agatcttgcc ggatgtttta gcccggctgc atgatcacct gctgctgcgg cgggatgaac
    15721 gcgggcgctg ggggctgcac gaatcggtgc ggcaatatgc ggctgaaaag cgcgggatgg
    15781 atgaacaaag cgaccgggaa gtttgcaatc gtcacagtcg gttttttgcg gaatatgtcc
    15841 accaaaggac ggggcagctt gagactctgg atcagggcga ggccttgaat gccattgcgg
    15901 ccaaccacga aaatattgtg gcgggatggg aaaacgcgat tgcaaaccgg caattagccc
    15961 ggcttgacca atatctgcgg gggctttacc tgttctatga ctttcgcagc cggtatcatg
    16021 atggagcggc cttattgggg cgcgcactga agcagttggc aggcgaaccc caggcggcac
    16081 cgctgtacgg acggatgctg gcgcgattgg gaattatgca gtcccggctt tcacaaaatg
    16141 acgaggcgct cgtcaccctg acagatgccc tgcatcaact ggccgacaca gaccaacccg
    16201 atgaacaggc tatctgtatg gtttggctgg cgaacctgat tcgcaaacga aagtcacagg
    16261 cagaagccgg tgaactggcc cggcaagcgc tggaaatatc acgaaaaatt gacta
//
LOCUS       DAOPVS010000033        16283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3635_length_16283_cov_56.991126, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000033 DAOPVS010000000
VERSION     DAOPVS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16283
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_16283_cov_56.991126"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKL11_04635"
     CDS             complement(<1..299)
                     /locus_tag="PKL11_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ12921.1"
                     /translation="MTITILLADDHHVVRQGLRSLLALERDFQVIAEASDGLEAVRTI
                     DRLRPQVSIVDLMMPGLGGLEVTRQVCQWTRVVILSMHDNEAYVIEALRNGAYGYV"
     gene            complement(296..2893)
                     /locus_tag="PKL11_04640"
     CDS             complement(296..2893)
                     /locus_tag="PKL11_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HNZ12922.1"
                     /translation="MSLRKTTLLAIVLTTVGLIAVLLFSQRMVLLPYFEQEQTQTVQL
                     NLQRALSALDAEFSYLTQISRDWAKWDDTYTYVQDRNTTYWRANLQLTTFRDLQLNLF
                     ALLDDEGQVVFAINYDLVTEKAEPLPEALTHQIQPGSQLLAAPDDLTGRQGFLDIDGR
                     ALLIVSHPVLPTDGVGPAQGTLLIGRYLDADNLDRISNSVQLPLTLYPANKAAPESAR
                     SALLKGDSYYMEALDDTSVAGYTLLKDINDQPAFLLRADQPQSVYRTGILLVNYLVVA
                     QVMSGITFSAILFLLLEMTVLGRLRRLSRDVTAIGASGDITRRVAVTRHDELTRLGQS
                     INATLDSLAQAQKQRQESEGRFRTLVESMDDVVFTVSNDLSAVETFGQKANAIGMKDI
                     LSPASASPSSAARLGHLQALQKALSGEQVSFEWSLPGSDQPRHFLTALSPIRSTDGAI
                     TGAVGVGREITERKQLEERLRHQVADLDALHMASQVLLGQLEPAETLAAICRLAVEQF
                     GLQAAWIALPRAEQQMLIPLHARGFQMEQLSVLHLHLNAADLVHPAVTACRTGQPQVV
                     APLYSGESDAQTAAGAALRALACLPLPQNDQPGGVLAVYSAQENFFSPEKMQLLQAFT
                     NLASATLQNASLFEQVSRGRERLQDLSRRLVDIQEGERRRIALELHDQIGQILTGLKL
                     LLDSSAALPAVQLKPRLEQAQELANDLISRVRQMSLNLRPSLLDDLGLVPALLWLFER
                     YTAQTNVGVVFQHTAVEGIRFPIQIETAAYRLVQEALTNVARHAGVNEVIVRLWRADD
                     RLGVQVEDAGCGFNPADILNGESSGLLGQRERLGLLGGRLTIDANPGSGACLTAELPL
                     NGQPLEEEE"
     gene            3218..3508
                     /gene="groES"
                     /locus_tag="PKL11_04645"
     CDS             3218..3508
                     /gene="groES"
                     /locus_tag="PKL11_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNZ12923.1"
                     /translation="MHLKPLGARVVVEPLEAEEVTAGGIVLPETAKEKPQRGKVIAVG
                     AGDRDENGKRIPMEVSVNDTVLFAKYSGTEIKIDGKKLLILRESDLLAIIEG"
     gene            3551..5185
                     /gene="groL"
                     /locus_tag="PKL11_04650"
     CDS             3551..5185
                     /gene="groL"
                     /locus_tag="PKL11_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNZ12924.1"
                     /translation="MGAKQIIFAEEARRKLKAGIDVVATAVATTLGPKGRNVALDRKY
                     GSPTITHDGVTVAKEIELEDPFENMGAQLLKEAATKTNDIAGDGTTTSTVLAHAIVTE
                     GLKTLAAGANPMLLKRGIEAASKLVADEIRKQAIEITTKTDIASVATISAQDKEIGAL
                     IADVMDKVGKDGVITVEESKGLQFETEYVEGMQFDRGYISPYFITDPDHMEATIQEPY
                     ILVYDRKISAAADLVPVLEKMMQLGKRELVIIAEDVDSEALATLVLNRIRGILNVLAI
                     KAPGFGDRRKAMLQDIAILTGATVISDEMGRKLETATIADLGRAEKVVSDKDNTTLIG
                     GKGEEGAIHARIEQIRVEIDKSTSDYDREKLQERLAKLAGGVAIIRVGAATETELKEK
                     KHRVEDALSATRAAVEEGIVPGGGVALVNAIAALEKLKLGVEDEQIGVNIVRKSLEVP
                     MRRIAENAGMEGSVVVENVRQQQKKAKNAHIGYDVLSGEYIDMVKSGVIDPAKVTRGA
                     LENAASIAAMILTTEALITDAPEKDKPAAAPQMPDY"
     gene            5269..6066
                     /locus_tag="PKL11_04655"
     CDS             5269..6066
                     /locus_tag="PKL11_04655"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNZ12925.1"
                     /translation="MSRPTLRTLWKPLLVGFGAAVLILWLAFAPPGLLGKADAVGYAV
                     CHRITERSFMLGDRAMPVCARCSGTFLGALLGLAYQLPRGRRGKLPPVSICIALGVLA
                     LAWAFDGFNSYMHFFEGAPHFYEPQNWLRLATGTGLGLGMALVLLPIMHQTFFTTFDD
                     RPAIPNWGAFGGLLGLAALLNLVILTENPLVLYPLAVLSSVSVLTLLTLCYSLLLVIF
                     LKRDGLANTWRDVWPALVGGLTIALLQTLVIDAVRLYLTGSWGGLGI"
     gene            6305..7000
                     /locus_tag="PKL11_04660"
     CDS             6305..7000
                     /locus_tag="PKL11_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ12926.1"
                     /translation="MLTDKLMYWFSKPLVIAYSDTMLRMNVKRHTDLPHGPKIIAANH
                     PSTTDPFFVAGMLRHQSYILINELLFQVPILGEYLRRSGHIPVEAGNGPAAIDAAVQR
                     LREGKTVIIFPEGDLSPSAQGFHPARTGVARIALLSGAPVIPVGINLNWGLNRTVKST
                     VRGKVSYGRWYLRGPYQVTVGKPLQFNGDVEDRPHVRKVADRVMHEIIELAHESEMRM
                     NQSPSLPLLPEIQ"
     gene            complement(7077..8027)
                     /locus_tag="PKL11_04665"
     CDS             complement(7077..8027)
                     /locus_tag="PKL11_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12927.1"
                     /translation="MKIKTLLRLALAAVLLALISAQVAPLPAARAQTQPAVQLAAPGY
                     IVVDHTTTDLDLIPPEWIAAAQELAVHYAHTSHGSQITSGLAALEEDHPLYAYEVDYA
                     GSTPPTTAPATPPLWIFDGNPPETYIEPGDYWEGEAGINRTRAVINTGLFDYSMWSWC
                     GQAGYYETSQIQTYLDVMADLEADYPNTRFILMTGHTVGGGSTLLETNNNLIRQFAQT
                     HGMILFDFNDIERYTPTGDGPYNNIDDGDCEWCANWCDAHPSDCEHLPVGDCAHSDTS
                     EDAELFCSLKANAFWWLMARLAGWPGPGPLTYVYLPTILR"
     gene            complement(8140..9516)
                     /locus_tag="PKL11_04670"
     CDS             complement(8140..9516)
                     /locus_tag="PKL11_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNZ12928.1"
                     /translation="MNTYRKSERGQIVVIFVVALVALLALTALAIDGSMIMSDRRFAQ
                     AGADAAALGGAGAAAREIENAGITYSTFDCSSAAVLDAIDAASQAAIDAAAANNFLGL
                     ANDGDLNPNNETYGVEVLCEDDAASVDRHIDVMTTITSETSTSFLHLFNSGPMTNTVT
                     AVVRVRPPTPLAMGNAIVTFSPLWGTKTGGLDFTGGAVLHVTDGGMFSNSSIRQKGSG
                     SIWVHDGLGDVMDGSVRCLMDCDEFVPGNMDPDPVDICTAADNCPGLRIQPITMPTPA
                     CGANRGAINGSGTYDPGTYSSASIGNGDVVKLNPGLYCFNGVVKITGGSLTTVADADG
                     HLGVTIFLKKANGDWNITGGTITLVSPVNADQVTAGSIQGVLLYLEDGNTNEIKITGN
                     DKGCTGSYSGLIYGRDAGLYVRGNAADTFNGQILVRYMDNAGTSDMKVSFVDNTNFGD
                     PTFLDVIR"
     gene            complement(9556..10083)
                     /locus_tag="PKL11_04675"
     CDS             complement(9556..10083)
                     /locus_tag="PKL11_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNZ12929.1"
                     /translation="MKSQPSMNPNKSERGQSLVELAISLIILLIILAGVVDLGNMMFQ
                     YMAMRDAAQEAASYGSIFPRDCADIEDRARASLFNAGEYDPDTNPDGIIITIIVTNTT
                     ESPAVQKFCVDAAAYEDPTNEIDLDACNPHGITVTVEQPNFPITMPLLGTFLGRQTVH
                     LEASMTDTILRPACP"
     gene            complement(10097..12382)
                     /locus_tag="PKL11_04680"
     CDS             complement(10097..12382)
                     /locus_tag="PKL11_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12930.1"
                     /translation="MKRTFLSLLLICLLTTGIQTARAQTPAPEGDFGYTVGYVTYDWE
                     ELQGQADVGEVLFLSYDDALSCAIDQTGYCTPTPIPLGLDFPFFENTYSELYVSINGL
                     LLFGGSQSDPYLVNRPIPRDFSPQDIIAPYWDDLELDPVDGAVYYKTFANCPVGDSAS
                     ACAIIEYYHAKKYAEASAPTFNFEIVLYPSGNLRIGYQSLPLTLDECTVGIEDDQGIS
                     GNQVYYNQTGLQPATMLQFTYPAPGYRLKAFPVVSGAFLNNYQVDLPFTLRNAGTFQD
                     TYTLSYTLLPGSQSPADGWTFTFIDSTGAPITGGVTLSPGLLSEQEIRLRVTAPDTAL
                     RGDYARIQIDIDSPNVSPFTIYRQAAAPAPFTSVSLDTNRVETRFQTLLRNYTGAVFL
                     GYTGGQVAIAHQSEAIYLPYWTRLGENQTGLRYYNTVVARVNAVGNRIGPRPLDDHRY
                     DVLGVRDEEAYLAMAPNGSGAAVFVRRTTQPLTSVDLIRVSSSGSPIGEPLTLMDNFG
                     SDGRQYNRPVVTALSDNRFVVVWTFFDVNELDESLSDLYYAVLDSAGNVIHSPAPITN
                     AVYGGLLYSDASVVTMAGDNVLVVYQQYNETTGDASLLYRILDPDTGPGAQQALATAA
                     QPSIADIAVLDQNNILVAWVDRGASDDFYQAQYAVLNASASGFVLPPTVLAQPDQRPA
                     TRLSVTADSQGNGVLVWGEVYTRRLYYTLVNSSGTVLTPPMTFRQAVPDRLLLLASEG
                     GSLAYCPDVLTWKLTLPAVLK"
     gene            complement(12389..14644)
                     /locus_tag="PKL11_04685"
     CDS             complement(12389..14644)
                     /locus_tag="PKL11_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNZ12931.1"
                     /translation="MKRLRIGANHVRWQIKSLRGQGMVEFALALPVFLLLVLGIMEFS
                     WMLFHYSITFSASREAARYGSSVGLGATNLSRDIDCVGIRAEAFRVGRPAGITTNGQV
                     EIRYDLGPDDTRTWTSLPSCDNPTKPNTLLGWRIAVRVTADHNSLIGIFPSFPITSTT
                     FRTIIKNVDMTANPTAPAGQHTNTPTYTVSPTVTPTPTETQPPTATFTPLPQVDITVS
                     KNDGTSFYIPGGTVTYTIIITNAGPNDANGCKLEDNRPYQVTTWDWTCTTAGGASGCD
                     AYSGSGNILDEGLNIPVGGSITYTVTANLNVAATDYLINYASIILPPGMGDDNGGLTA
                     VDIDWPPAADLSIAAISQENYYIPGQDFTFSFVVANAGPDAANGALVTAEKPTQVESY
                     TWTCVASGGASCHNQAAAQPTFSDTMDYMPAGGSVTYTVNARITATATGVMTFGVNVA
                     PPMGITDLNMDNNAITITATPPTANIWISISDGNIANYPRGTLTYTVKVYNWGPEDVT
                     NVFIISAFPDPQVISKWDWVCSGATGGASGCTGVTNSSWEFSDYIDIPAGGQITYSVT
                     VTILNTAMSPLYIVVDESVPAPFMDPDDPALQNDNNSATDINDPPVFSCNNLTNDTSS
                     YTNQKMTVNITNNTSYTGMISSIQVDFDDHKDKKYSRLDSISFGGGGIWTGPLSETKG
                     TPVTISNWNAGSETNRTLDFAGSGSLSTKLLSLNFYQDYNKLDIAIVNVRLAFQTPTG
                     EVICTLGNLAP"
     gene            complement(14831..15724)
                     /locus_tag="PKL11_04690"
     CDS             complement(14831..15724)
                     /locus_tag="PKL11_04690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ12932.1"
                     /translation="MIDVSICIVSYNTCSLLQDCLKSLFETPTRAAFEVIVVDNASTD
                     GSPTMVASQFPQVAVIQLDSNQGYTRPMNLGLQRGRGRYLMQLNPDTVVQPGWLDELL
                     AFIEAHPEVGICTPKVMNRDGSLQRQCRRSAARPWDVFTYFSGLWRIFPNNPRFGGYL
                     MTHMDADATHPAEAVSGSCMLIRRAVVEQVGYLDEQFFAYQEDTEFCFRTRQAGWQIY
                     YVPAARIIHYGGQGGSRVQPFRAILQWHRSYYLYYRKHLAKDYCFLINGLMVAGMAAK
                     LGLSLLVNLLRREKIVGSKKP"
     gene            complement(15721..>16283)
                     /locus_tag="PKL11_04695"
     CDS             complement(15721..>16283)
                     /locus_tag="PKL11_04695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ12933.1"
                     /translation="LPALLARLDINLAPLVLDNPFSQSKSEIKFMEAALVGVPTLASA
                     TNAFQFAIRSGQNSLLIHQDTDWFAGLQALMDPQFRQALGRQARADVLTQYTPPERSR
                     SAVETLNAVGLHLNRPLGLEFCPNPAQDPTQLNWPPEADAYPALTQMALYSLRRRGPG
                     TLFKQVWIYLRRLLSRWIPYGGKAAR"
BASE COUNT         3636 a         4290 c         5066 g         3291 t
ORIGIN      
        1 acgtagccat aggcgccgtt gcgcagggcc tcaatcacat aggcttcgtt atcgtgcatt
       61 gagaggatga ccacccgggt ccactggcaa acctgccggg tgacttccag tccgcccaaa
      121 ccgggcatca tcagatccac aatggagacc tgcggacgaa ggcggtcaat ggtgcggacg
      181 gcttcgaggc cgtcactggc ttcggcaatg acctgaaaat ccctttccag ggccagcagc
      241 gagcgcaatc cctgccgcac cacatgatga tcatcggcca gcaaaatggt aatggtcatt
      301 cttcttcctc cagcggctgg ccgttgagcg gcagttcagc cgtcaggcag gcgccgctgc
      361 ccggattggc atcaatggtc aggcggccgc ccagcagccc caggcgctcg cgctggccca
      421 gcagaccgct ggactcgcca ttgaggatat cggcaggatt aaagccgcag ccggcatctt
      481 ccacttgaac ccccaggcgg tcgtcggcgc gccagagtcg gacaatcacc tcattgacgc
      541 cggcatggcg ggcaacgttg gtcagtgcct cttgtaccag ccggtaggca gcggtttcaa
      601 tttgaatggg gaagcggatg ccttctaccg cggtatgctg gaagaccact cccacgttgg
      661 tttgagcggt atagcgctca aacaaccaga gcaaggccgg caccagtccc agatcgtcca
      721 gcagggaggg gcgcaggtta agcgacatct gccgcacccg gctgatgagg tcattggcca
      781 gttcctgggc ctgttcgagg cgcggcttca attgaaccgc cggcagggcg gccgaagaat
      841 ccagcagcag tttgagcccg gtgagaattt ggccgatctg gtcgtgcaat tccagggcaa
      901 tccggcggcg ctcgccttcc tgaatatcca ccaggcggcg ggagaggtct tgcaggcgtt
      961 cccgcccgcg cgaaacttgc tcaaagaggc tggcgttttg cagcgtggcg ctggcgagat
     1021 tggtaaaggc ctgcaaaagc tgcattttct cggggctgaa gaagttttct tgcgcgctgt
     1081 acaccgccag cacgccgccg ggctggtcgt tctgcggcag gggcaggcag gccagggcac
     1141 gcaaagctgc accggcagcc gtctgggcat cactttcgcc gctgtaaagc ggggcaacca
     1201 cctgcggctg gcctgtacgg caagccgtga ccgccgggtg aaccagatcc gctgcattca
     1261 agtgaagatg gagaacgctg agctgctcca tttgaaagcc ccgggcgtgc agcgggatca
     1321 gcatctgttg ttcggcgcgc ggcagggcaa tccaggcagc ttgcaggcca aactgctcca
     1381 ctgccaggcg gcagatggcc gccagggttt cagcaggttc aagctggccc aacaacacct
     1441 gcgaagccat atgcagggca tccagatcgg caacctggtg acgcaggcgt tcttccagtt
     1501 gtttgcgctc ggtgatttca cgccccaccc ccacggcacc agtgatggca ccatcggttg
     1561 agcgaatggg ggaaagcgcc gtgagaaaat gacgcggctg atcggagccg gggagtgacc
     1621 attcaaagga gacctgctct cccgacaggg ctttctgcaa agcctgcaaa tgccccaggc
     1681 gcgcggccga tgatggcgat gcgctggcag gcgacaggat atctttcata ccaatggcat
     1741 tggccttttg accaaaggtc tctacagccg acaggtcatt gctgacggta aagaccacat
     1801 catccatgga ttcgaccagc gtccggaagc ggccctcgct ttcctggcgc tgcttttggg
     1861 cttgagccag cgaatcgagg gtggcgttga tagattggcc caggcgggtc agttcatcgt
     1921 ggcgcgtgac ggccacccgc cgggtgatat cacccgaggc gccaattgcc gtcacatccc
     1981 gactgaggcg gcgcagtcgg cccaacaccg tcatttccag cagcagaaag agaatggcgc
     2041 tgaaggtaat gcccgacatc acctgagcca ccaccaggta attgaccagc aggatgccgg
     2101 tgcgataaac cgactggggc tggtcggcgc gcagcaaaaa ggcgggctgg tcattgatgt
     2161 ctttcagcag ggtgtaaccg gctactgagg tgtcatccag agcttccatg taataagagt
     2221 cgcctttcaa gagcgcgctg cgggcagatt cgggagcagc tttgttagcc gggtagagtg
     2281 tgagcggcaa ctgcacggaa ttcgagatgc gatccaggtt gtcggcatcc aggtagcggc
     2341 cgatcagcag tgtgccctgg gccgggccaa caccatcggt tggcagcacc gggtgagaga
     2401 cgatcagcag cgcccggccg tcaatatcca aaaagccctg acgccccgtc agatcatcgg
     2461 gcgcggccag cagttggcta ccgggttgaa tctgatgggt cagggcctcg ggcaggggct
     2521 cggccttttc ggtcacgaga tcgtaattaa tggcaaaaac gacctggcct tcatcatcca
     2581 gcagggcaaa cagattgagc tgcagatcgc gaaaggtggt tagctgcagg ttggcccgcc
     2641 aatacgtggt attgcgatcc tggacatagg tgtaggtatc atcccacttg gcccaatcgc
     2701 ggctgatctg ggtgaggtaa gagaactcgg catccagagc gctgagcgca cgctgcaaat
     2761 tgagctggac ggtctgggtt tgctcctgtt caaagtaggg cagcaagacc atgcgctgcg
     2821 agaaaagcag cacggcaatg agacccaccg ttgtcagaac gatggccaac agcgtggttt
     2881 ttcgaaggct catgagcggg ttcctgaagt tgacgatcca accgagcgtt tattcttatt
     2941 aaaaagcaat taaaacaaat cagttaatta taccgagggg atgatagaat cgggttcggg
     3001 tttgagggca aatcagtttg caaaaatgca aggcggaaag gattagacag ggggttttct
     3061 caaaatctgt gtatgagttt tataagaacc ttgaggtttc tcttgactct gatatagaag
     3121 gtgagtatga taggggatgt tctggcagtg ttggcaaaag agtgctaaaa ctgcaaaaaa
     3181 tagatctcaa atcctgaatc aggaggaaac aatggcaatg catcttaaac cccttggcgc
     3241 ccgtgtggtt gttgaaccgc ttgaggccga agaagtgacc gctggtggta tcgttctgcc
     3301 cgagaccgcc aaagaaaagc cgcagcgcgg caaagtgatc gccgtaggcg cgggcgaccg
     3361 cgacgaaaac ggcaagcgca tccccatgga agtttctgtc aacgacacgg tgctgttcgc
     3421 caaatactct ggcaccgaaa tcaagattga cggcaagaaa ctgctgatcc tgcgcgagag
     3481 cgacctgctc gccatcattg aaggttaatt tcggttattt ttcaagttaa accattaagg
     3541 agatttcaca atgggtgcta aacaaatcat ttttgctgaa gaagctcgcc gcaaactgaa
     3601 ggcgggtatt gatgttgtgg cgaccgccgt ggcgaccacc ctgggcccca aaggccgcaa
     3661 tgtggccctg gatcgcaaat acggctcccc caccatcacg cacgatggcg tgaccgtggc
     3721 gaaagaaatt gaactggaag accccttcga aaatatgggc gcccagcttc tgaaggaagc
     3781 cgccaccaag accaacgaca tcgctggcga tggcaccacc acctcgaccg tgctggcgca
     3841 cgcgattgtg accgaaggtc tgaagaccct ggccgctggc gccaacccca tgctgctgaa
     3901 gcgtggcatt gaagccgcct cgaaactggt ggccgatgaa atccgcaagc aggcaattga
     3961 gatcaccacc aagaccgaca tcgcctcggt ggccaccatc tcggcgcagg acaaagaaat
     4021 cggcgccctg atcgccgatg tgatggacaa agttggtaaa gacggcgtca tcacggttga
     4081 agaatccaag ggcctgcagt ttgaaaccga atacgttgaa ggcatgcagt ttgaccgcgg
     4141 ttacatttcg ccctacttca tcaccgatcc cgatcacatg gaagccacca tccaggaacc
     4201 ctacatcctg gtctatgatc gcaagatctc tgcggctgcc gatttggtgc ccgtgctgga
     4261 aaaaatgatg cagcttggca aacgcgagct ggtgatcatt gctgaagacg tggacagcga
     4321 agcactggcg accctggtgc tcaaccgcat ccgcggcatc ctgaacgtgc tggccatcaa
     4381 ggcccctggc tttggtgatc gccgcaaggc catgctgcag gatatcgcca tcctgaccgg
     4441 cgccaccgtc atctctgacg aaatgggccg caagctggaa accgccacca tcgccgacct
     4501 gggccgcgct gagaaggttg tgtctgacaa ggacaacacc accctcatcg gcggcaaggg
     4561 cgaagaaggc gccatccacg ctcgcatcga gcagatccgc gtggagattg acaagagcac
     4621 cagcgattac gatcgcgaaa agctgcagga acggctggcc aaactggctg gcggcgtggc
     4681 catcatccgt gtgggtgcgg ctaccgagac cgagctgaaa gaaaagaaac accgcgtgga
     4741 agatgccctg tcggccaccc gcgctgcggt tgaagaaggg attgtccccg gtggtggtgt
     4801 tgccctggtg aatgccattg ctgccctcga gaaactgaag ctgggcgtgg aagacgaaca
     4861 gattggcgtc aacattgtcc gcaagtcgct ggaagttccg atgcgccgca tcgctgagaa
     4921 cgctggcatg gaaggctctg tggtggtgga gaatgtgcgc cagcagcaga agaaagccaa
     4981 gaatgcccac atcggttatg atgtgctgag cggcgaatac attgacatgg tcaagagcgg
     5041 tgtgattgat cccgcgaagg tgacccgtgg tgcgctggaa aacgccgcct cgattgccgc
     5101 catgatcctg accaccgaag cgctgatcac cgatgctcct gaaaaggaca agccggccgc
     5161 tgcgccccag atgcccgatt actaaatcgg cgactgaatc aaaccgtaag caaggcagcc
     5221 ccctcccctg gagggggctg ttggtttgaa agctgatata atgctcttat gagccgccca
     5281 accctgcgca cactctggaa accgctgctg gttggttttg gcgcggcggt gttgatttta
     5341 tggctggcgt ttgcgccgcc cgggctgctg gggaaagcgg atgccgtggg gtacgcagtc
     5401 tgtcaccgga ttacggagcg ctcgtttatg ctgggcgacc gcgccatgcc cgtctgtgcg
     5461 cgctgctcgg ggacgttttt gggggcgctg ctggggctgg cctatcaact gccgcgcgga
     5521 cgccggggca agctgccgcc ggtgagcatt tgcattgcgc tgggcgtgct ggcgctggcc
     5581 tgggcttttg acgggttcaa ttcgtacatg catttttttg aaggcgcgcc gcatttttac
     5641 gagccgcaga actggctgcg gctggcgacc ggcaccgggc tggggctggg catggcgctg
     5701 gttctgctgc caattatgca ccagacgttt ttcaccacat ttgatgaccg cccggccatc
     5761 cccaactggg gggcgtttgg cgggctgctg gggctggcgg cgctgctcaa tctggtcatt
     5821 ctgaccgaaa atccgctggt gctgtacccg ctggcggtgt tgagcagcgt cagtgtgctg
     5881 acgctgctga cgctgtgtta cagcctgctg ctggtgatct tcctgaagcg cgatggactg
     5941 gcaaacacct ggcgcgatgt ctggccggcg ctggtgggcg ggttgacgat tgcgctgctg
     6001 caaaccctgg tgattgatgc ggtgcggctg tatttgaccg ggtcgtgggg cgggctgggg
     6061 atttaggatt attgattggg gagttttcaa aattgaaagc cggggaaatg gggcgtatcg
     6121 aagcggcacc gggcggggtt ttagtattca gcagcaggtc ataatgacct gcaaaacaaa
     6181 atatgactcg tcacgcccgg tcaatatgcc ccacaggcga tactcaccgg cgggcaatcc
     6241 ggtaaactct aaccatcatc atcccctgca cgacacccaa attaccaact gaggattaca
     6301 agcgatgctg acagacaaac tgatgtactg gttttccaag ccactggtca ttgcttacag
     6361 cgacaccatg ctgcgcatga acgtgaaacg gcatacagat ctgccgcacg gccccaaaat
     6421 cattgctgcc aaccacccca gcaccaccga ccccttcttt gtggcgggca tgctgcggca
     6481 tcaatcgtac atcttgatta atgagttgtt gttccaggtg ccaatcctgg gtgaatacct
     6541 ccgccgctcg gggcatattc ccgtagaggc gggcaacggc ccggccgcca ttgacgcggc
     6601 agtgcagcgg ctgcgcgagg gcaagaccgt gatcatcttc cccgagggcg atctgagccc
     6661 ttcggcgcag ggctttcacc cggcgcggac gggcgtggcc cgaattgccc tgctgagcgg
     6721 cgcaccggtg atcccggtgg gcatcaacct gaactggggc ctgaaccgga cggtcaaatc
     6781 tacggtgcgg ggcaaggtgt cttacgggcg ctggtatctg cgcgggccct accaggtgac
     6841 ggtgggcaag ccgctgcagt tcaacggcga tgtggaagac cgcccgcatg tgcgtaaagt
     6901 ggccgaccgg gtgatgcacg agattattga actggcgcac gagagcgaga tgcgcatgaa
     6961 tcagagcccc agcctgccgc tgctgcctga aattcagtag ccgggcatta ggccctgggg
     7021 tttagaccca tagaatgatt atcgggtgtg gccttttgac cacacccgat tgtttttcag
     7081 cgcagaatgg tggggagata gacgtaggtg agggggccgg ggccgggcca gccagccagg
     7141 cgagccatga gccaccagaa agcattggct ttgagggagc agaaaagctc ggcatcttca
     7201 ctggtatcag aatgggcaca atcgccaacc ggcagatgtt cgcagtcaga ggggtgggca
     7261 tcgcaccagt tggcgcacca ctcgcaatcg ccatcatcga tgttattgta ggggccatct
     7321 ccggtgggag tgtagcgctc gatatcatta aaatcaaaca agatcatgcc atgggtctgg
     7381 gcaaactggc ggatcaggtt gttgttggtc tctaacaggg tgctgccacc gccaaccgta
     7441 tgcccggtca tcaggataaa acgtgtattg gggtagtcgg cttccaggtc ggccatcaca
     7501 tccagatagg tttggatctg ggaagtctca tagtaaccgg cctggccgca ccaggaccac
     7561 atggagtaat caaaaaggcc ggtattgatc acggcgcggg tgcggttgat gccagcttcg
     7621 ccctcccagt aatcgccggg ttcgatgtag gtttcgggcg gattgccatc aaaaatccac
     7681 aggggtgggg tggcgggggc cgtggtgggt ggggtactgc cggcataatc aacttcatat
     7741 gcgtacagcg ggtggtcctc ctccagtgcc gccaggccag acgtgatctg actgccgtga
     7801 gaggtgtggg cgtaatggac ggccagttcc tgggcggctg caatccattc cggggggatg
     7861 aggtcgaggt cggtggtggt atgatccacc acaatgtagc caggggcggc aagctgaacc
     7921 gccggctggg tctgcgcccg ggcagccggg agcggtgcca cctgcgcgct gatcaacgca
     7981 agcagcaccg ccgccagcgc caggcgaaga agggttttta ttttcatggg ggtctccaaa
     8041 aatggggaaa gaatgcggtg gaatgaaaag gggcggagcc acggctgcgc gctgtgactc
     8101 cgcccataat aatcggtgtt taatttgcgg gttgccaggc taacgaatca cgtccaaaaa
     8161 ggtgggatcg ccgaagttgg tgttatccac aaaactcact ttcatatcac tggtaccggc
     8221 attatccata tatctcacca agatttgccc attgaaggtg tcagccgcat tgccacgaac
     8281 gtaaagaccg gcatcccgtc catagatgag acccgagtac gagcccgtgc agcccttgtc
     8341 attgccggtg attttaattt catttgtatt gccatcttca aggtaaagca gaacaccctg
     8401 gatggagcct gcagtcacct gatcggcatt aaccggagaa accagagtga tggtaccccc
     8461 cgtgatattc cagtcaccat tggctttttt caggaaaatg gtcacaccca agtgcccatc
     8521 tgcatccgcc acggtggtga gtgagccacc ggtgattttt acaacgccat tgaagcagta
     8581 caggcccgga ttcaatttaa ccacatcccc attgccaatg ctggcactgg aataggtccc
     8641 gggatcatag gtgccggagc cgtttatggc accccggttt gccccgcagg caggggttgg
     8701 catcgtgatc ggctgaatgc gcagacccgg acaattgtcg gctgccgtgc agatatccac
     8761 cgggtccgga tccatatttc cggggacaaa ttcatcacaa tccatgaggc agcgcacgct
     8821 gccatccatc acatccccca gaccgtcatg gacccagatg ctgccgctcc ccttctgacg
     8881 gatgctcgag tttgagaaca tgccgccatc ggtcacatgc agtaccgcac cgccggtgaa
     8941 atcgaggccc ccggtcttgg tgccccagag cgggctgaag gttacaatgg catttcccat
     9001 ggcgagaggt gtgggtgggc gcacccgcac cacggcagtg accgtattgg tcatgggccc
     9061 tgaattgaac agatggagga aggatgtgga ggtttctgat gtaatggttg tcatcacatc
     9121 aatatgccgg tccacgctgg ccgcatcatc ctcacagaga acttccacac cataggtttc
     9181 attattggga ttaaggtcac catcattggc aagaccaaga aaattattgg ccgccgccgc
     9241 gtcaatggcg gcctgggaag ctgcatcaat ggcatccagc acggctgcag aggagcagtc
     9301 aaaagtgctg taggtgatgc ctgcattttc aatctctctg gctgccgccc cggcgccgcc
     9361 caacgcggca gcatcggctc cggcctgggc aaaccgccgg tcagacatga tcatactccc
     9421 gtcaatggcc agagccgtga gcgccagcag cgccaccagc gcaacgacaa agatgaccac
     9481 aatctggccg cgttcggact tgcgataagt gttcatggtt ttcctccttg gaaaggacat
     9541 acaaataatg ggtgtttagg gacaggccgg gcgcaggata gtatccgtca ttgaggcctc
     9601 caggtgaacc gtctgccggc ccaaaaaggt gcccagcagg ggcatggtga tggggaaatt
     9661 gggctgctca accgtcacgg taatgccgtg ggggttacag gcatccaggt caatttcatt
     9721 ggtcgggtct tcataagcgg cagcatcgac acagaatttt tggacggccg ggctttcggt
     9781 agtattcgta acaatgatgg tgataatgat gccgtcggga ttcgtgtcgg ggtcatattc
     9841 accggcgttg aagaggctgg cgcgggcccg gtcttcaata tcagcgcagt cgcgggggaa
     9901 aatggagccg tatgaggcag cttcctgggc ggcatcgcgc atggccatgt attggaacat
     9961 catattgccc aggtccacca ccccggcgag gatgatgagc agaatgatca gactgatggc
    10021 cagttcaaca aggctctggc cgcgttcaga tttatttgga ttcatagagg gttgggattt
    10081 catcacaatc tccagattat ttcaaaaccg caggcagagt aagtttccag gtgagcacat
    10141 cgggacagta ggccaggctg ccaccctccg aggccaggag gaggaggcga tcggggacgg
    10201 cctgacggaa cgtcatgggg ggggtcaaaa ctgtgccgga tgaattgacc agcgtatagt
    10261 agagacgccg ggtgtaaacc tccccccaga ccaggacgcc attgccctgc gaatcagcag
    10321 tcactgagag acgggtggcg gggcgctggt cgggctgtgc caggacagtg gggggcagca
    10381 caaaaccaga ggcgctggca ttcagcacag catactgcgc ctgatagaag tcatcagagg
    10441 caccccggtc cacccaggct accaggatat tgttctgatc caggacagct atatcagcaa
    10501 tggaaggttg agcggccgtc gccagggctt gttgggcacc ggggccagtg tcgggatcca
    10561 gaatgcgata gagcaggctg gcatctccgg tggtttcgtt gtattgttga tagactacca
    10621 aaacgttatc tccggccatg gtgaccacag aggcatcact gtagagcaag ccgccataaa
    10681 cagcgttggt gatgggcgcc ggcgagtgaa ttacattgcc ggcgctgtct aagaccgcat
    10741 agtaaagatc gctcaatgat tcatctaatt cattgacatc aaaaaatgtc cagacaacca
    10801 caaagcgatt atcagaaagc gctgtaacca ccgggcggtt atattggcgg ccgtcagaac
    10861 caaaattatc cattaaagtc agtggttcgc ctataggact gccggaggaa ctgacccgaa
    10921 tcaagtcaac cgaggtcaga ggctgggtgg tgcggcggac aaaaactgct gcgccagaac
    10981 cattgggggc catggccaga taggcttctt catcgcgaac gcccaggaca tcatagcggt
    11041 gatcatccag tgggcgaggg ccaatgcggt tacccacggc attgacgcgc gccacaacgg
    11101 tgttgtaata tcgcagaccg gtttgatttt caccaagccg cgtccagtag ggcaggtaaa
    11161 tggcttcgga ttggtgggca atggccacct gtccgccggt atagcccagg aacactgccc
    11221 cggtgtagtt gcgtaagagc gtttggaaac gagtttcaac gcgattggta tccagtgaaa
    11281 ccgatgtaaa gggggctggg gcggcggcct ggcgatagat ggtaaaggga gagacgttgg
    11341 gggagtcgat gtctatctga atccgggcgt aatcaccgcg cagggcagta tcgggtgcgg
    11401 tcacgcgcag ccggatttcc tgttcagaga gaagcccggg ggacaaggtt actccgccgg
    11461 taatgggcgc gcccgtgctg tcaatgaagg tgaaagtcca gccatcagcg gggctctggc
    11521 tgcccggcag gagcgtataa cttagtgtat aggtatcttg aaaagttccc gcgttgcgca
    11581 gggtgaaggg caggtccacc tgataattat tcagaaacgc gcccgacacc accgggaagg
    11641 ccttgaggcg gtaacctggg gccgggtagg taaattggag catggtcgca ggctgcaaac
    11701 cggtctggtt ataatagacc tgattgcccg atatgccctg gtcatcctca atacccacag
    11761 tacattcatc cagggtcagc ggcaggcttt gatagccgat gcgcaaattg ccactggggt
    11821 acagaacaat ttcgaagttg aaggtggggg cactggcttc agcgtatttc ttggcgtggt
    11881 aatactcgat gatggcgcag gctgaagccg aatcacccac cgggcagttg gcaaaggttt
    11941 tgtaatagac ggcgccatcc accggatcca gttccaggtc atcccagtag ggtgcaatga
    12001 tatcttgagg cgagaagtcg cgggggatgg ggcggttgac aaggtagggg tcagactgag
    12061 agccgccaaa cagcagcagg ccgttgatcg agacatacag ctcagagtag gtattttcaa
    12121 agaatgggaa atccaaaccc agggggatgg gggtgggcgt gcagtaaccg gtctggtcaa
    12181 tggcgcagct cagggcatca tcataactaa gaaacaaaac ttcgccaacg tcggcctggc
    12241 cctgcagttc ttcccaatcg taggtgacat agccaaccgt atagccaaag tcgccctcgg
    12301 gcgcaggagt ctgcgcccgg gcagtttgga tacctgttgt caacaggcag ataaggagaa
    12361 gcgagagaaa ggtgcgtttc atcatggttt atggcgccag gttgcccagg gtacaaatca
    12421 cttcgccggt cggggtctgg aaagcaagcc tgacattgac aatggcgatg tccaacttat
    12481 tgtagtcttg atagaagttc agtgagagga gttttgtact caaactgcca gagccggcga
    12541 aatcgagggt ccgattggtt tcggatccag cattccaatt ggagatggta accggtgtgc
    12601 ctttcgtttc agacagcggg ccagtccaaa tcccaccgcc gccaaaggaa atcgaatcaa
    12661 gacggctgta ctttttgtct ttatgatcat caaaatctac ttgaatggac gagatcatcc
    12721 ctgtatagct ggtgttgttg gttatattga cggtcatctt ctggttggta tatgaggatg
    12781 tgtcattggt gaggttattg cagctaaaga ccggcgggtc gttgatatcg gtggcagagt
    12841 tgttgtcgtt ctgcagggcg gggtcatccg ggtccatgaa gggcgcaggg acgctttcat
    12901 caacaacaat gtacagaggc gacatggcgg tattcagaat agtgacggta accgaatagg
    12961 tgatttggcc gcctgctggg atgtcgatat aatccgaaaa ctcccaggaa gagtttgtca
    13021 ccccggtaca gcccgaggcg ccgcctgtgg cgcccgaaca aacccaatcc cacttcgaga
    13081 taacctgcgg atcggggaag gcgctgatga tgaatacatt ggtcacatct tcggggcccc
    13141 agttatagac tttgacagtg taagtgaggg tgccgcgcgg gtagtttgcg atgttaccat
    13201 ccgagatgct gatccagata ttggcggttg gcggggtggc cgtgatggtg atggcattat
    13261 tatccatgtt caggtcagtg atacccatgg gtggggcaac attgacaccg aaagtcatta
    13321 caccggttgc agtggcggta atgcgggcgt tgaccgtgta ggtgactgat ccgccagcgg
    13381 gcatataatc catcgtgtcg ctgaaggtgg gctgcgccgc ggcctggttg tggcaactag
    13441 cgccgccgct ggcaacgcag gtccaggtat agctttccac ctgggtaggc ttttcagcag
    13501 tgaccagggc gccatttgca gcatcggggc cggcattggc caccacaaac gagaaggtga
    13561 aatcctgccc gggtatgtag tagttttcct gagaaatggc agcaatgctc aaatcggcag
    13621 cggggggcca gtcaatatcc acggcggtca ggccgccgtt atcatcgccc atgccgggcg
    13681 gcaggatgat gctggcataa ttgatcaggt agtctgtggc cgcgacgttt agattggccg
    13741 tgacggtata ggtgatgctg ccgccaaccg ggatgttaag gccttcgtcc aaaatgttgc
    13801 cgctgccaga ataggcgtcg cagccgctgg cgccgccggc ggtggtacag gtccaatccc
    13861 aggtggttac ctggtagggg cggttatctt ccagcttgca gccattggca tcattgggcc
    13921 cggcgttggt gataatgatg gtataggtta ccgtgccgcc ggggatatag aagctggtgc
    13981 catcattctt gctgacggtg atatcaacct ggggcagtgg ggtgaaggta gccgtggggg
    14041 gctgggtttc ggtgggggtt ggcgttaccg tgggggagac ggtgtaggtg ggggtgttgg
    14101 tgtgttgacc ggctggcgcg gtggggttgg cggtcatatc cacgtttttg atgatggtgc
    14161 ggaacgtggt ggaggtgatg gggaagctgg ggaagatgcc aatcaatgaa ttgtggtcag
    14221 cagtcacccg cacggcaatc cgccacccca atagagtgtt tggtttggtg gggttatcac
    14281 agctaggcag ggaagtccaa gtcctggtgt catcagggcc tagatcatat ctgatctcta
    14341 cctggccatt ggtcgtaatg ccagccgggc ggccaacccg gaaagcctcg gcccggatac
    14401 ccacgcaatc aatatcgcga gataaattgg tggcacccaa accgaccgaa gagccgtagc
    14461 gggcagcctc gcggctggcg gaaaatgtga tgctgtaatg gaaaagcatc caggaaaatt
    14521 ccataatgcc cagcaccagc agcagaaaaa ccggcagtgc caaggcaaat tccaccatgc
    14581 cctggccgcg caacgattta atctgccatc gaacgtgatt tgcgcctatg cgcagtctct
    14641 tcatttgaga ctcctttccg catcattaag atacacgata agaacaggat tgaaaatccg
    14701 tacacctact taattttaca tcaaaccatg cttaatgatg cgctggattg caaaactgta
    14761 atgcaaatgt agagtgaaag ccattcaaac ccccaaaaat caggctaatc tgagcgccag
    14821 cgggttaaaa tcagggtttt tttgagccaa caatcttttc gcgccgcagc aggttgacga
    14881 gcaggctgag ccccagtttg gcagccatgc cggcaaccat caatccgttg atcagaaagc
    14941 agtagtcttt tgccagatgc ttgcgatagt agaggtaata ggagcggtgc cactggagga
    15001 tggcgcggaa aggctggacg cgtgaaccac cctgcccgcc atagtgaatg atgcgcgccg
    15061 cgggaacgta gtagatctgc cagccggcct gacgggtgcg gaagcaaaac tcggtatctt
    15121 cctgataggc aaagaactgc tcatcgaggt agcccacctg ctccaccacg gcccggcgga
    15181 tgagcatgca cgagccggaa acggcctcgg cggggtgggt ggcgtcggcg tccatgtggg
    15241 tcatcaggta gccgccaaag cgcggattgt tggggaagat ccgccagagg ccggagaagt
    15301 aggtgaagac atcccatggg cgggcggcac tgcggcggca ctggcgctgc aacgagccat
    15361 ctcggttcat cactttgggt gtgcaaatgc ccacctcggg gtgggcttct atgaatgcca
    15421 gcagttcatc cagccagccg ggctgcacca cggtatcggg gttaagctgc atgaggtagc
    15481 gaccgcggcc gcgctgcaag cccagattca tcggccgggt gtagccctga ttggaatcga
    15541 gctggatgac cgcaacttgc gggaactggc tggccaccat ggtgggcgag ccatcggtgg
    15601 aggcgttatc aaccacaatc acctcaaagg cggcgcgggt gggcgtctca aagagcgatt
    15661 tgaggcagtc ttgcagcagg ctgcaagtgt tgtatgaaac gatgcaaatg ctgacgtcta
    15721 tcatcgggca gccttccccc catagggtat ccagcgagag agcaggcggc gcaggtagat
    15781 ccagacctgc ttgaacaggg tgccggggcc acggcggcgc agggaataga gcgccatttg
    15841 cgtcagggct ggataggcat cagcctcggg gggccagttc agttgggtgg ggtcctgggc
    15901 gggattgggg caaaattcaa gccccagcgg gcgattgagg tgcagcccca ctgcgttcaa
    15961 ggtttcgacg gcgctgcggg agcgctcagg cggggtgtac tgcgtcagaa catcggcgcg
    16021 ggcctggcgg cccagggcct gccgaaattg gggatccatc agggcttgca aaccggcaaa
    16081 ccagtccgtg tcctgatgaa tcaacaggct gttttggcct gatcgaatgg caaactgaaa
    16141 ggcgttggtg gcgctggcaa gagttggcac gcccaccagg gcggcttcca tgaatttgat
    16201 ttcacttttc gattgggaaa aggggttgtc gagcaccagc ggcgccaggt taatatctag
    16261 ccgggcgagc aaagcgggca ggt
//
LOCUS       DAOPVS010000034        16040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3714_length_16040_cov_58.483516, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000034 DAOPVS010000000
VERSION     DAOPVS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16040
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_16040_cov_58.483516"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKL11_04700"
     CDS             <1..388
                     /locus_tag="PKL11_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12934.1"
                     /translation="GATNAILPDGNPVMPAIPFGTESIKTWCYYYEKAELALQEEDWR
                     TAYDMFTEGLGQGFSPSDGIEWLTLAQSALVLGDEAGLTAAAEGVESDPFARWQACTA
                     IHRMEEEGLSIDAANLTLSEQLFCQP"
     gene            complement(394..1074)
                     /locus_tag="PKL11_04705"
     CDS             complement(394..1074)
                     /locus_tag="PKL11_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNZ12935.1"
                     /translation="MQIEILYRPSYSVAQVRLNTGEAILAEPGAMVGMSRGIQVETRM
                     RGGLMQSLARAALGGESFFTNTYRAAAAGDEILLAPTLPGDVTVLNLQNQEFLVQSGS
                     FVASAETVTTDTKWTGARTFFGGEGLIMLRCSGAGELILSSYGAIHSIDLAAGQTYTI
                     DTGHLVAFERAMKYEIRTLGGIKSTLLSGEGLVVDLTGPGRVLLQTRNKRAFLDWLIP
                     QLPNKSNS"
     gene            1333..1617
                     /gene="rpsF"
                     /locus_tag="PKL11_04710"
     CDS             1333..1617
                     /gene="rpsF"
                     /locus_tag="PKL11_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNZ12936.1"
                     /translation="MRNYEVIVILNPELEESAVTALIEKIKGWIVESGGAVSQVDMWG
                     KRRMAYLIRKQREGQYVLLHAQFAPAFAAELERNLRFQEVILRFLISNVE"
     gene            1669..2073
                     /locus_tag="PKL11_04715"
     CDS             1669..2073
                     /locus_tag="PKL11_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ12937.1"
                     /translation="MSRGLNKVMLIGHLGRDPEMRYTPSGKPVTTFSVATSRSWSTAD
                     GERKTETEWFNIVAWGNLAEICKQYLTKGQQVYIEGRLQTRRWEDAEGVKHSSVEVVA
                     AEMMMLGERRDTNAGTTSTDEVADDIEDEYPF"
     gene            2121..2357
                     /gene="rpsR"
                     /locus_tag="PKL11_04720"
     CDS             2121..2357
                     /gene="rpsR"
                     /locus_tag="PKL11_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNZ12938.1"
                     /translation="MGGRRYMARPKICQFCADKNLKIDYKALDMLRRFVTEEGKIRPR
                     RQTGTCARHQRELAGAVKRARHIALLPYVEDRNG"
     gene            2440..3783
                     /locus_tag="PKL11_04725"
     CDS             2440..3783
                     /locus_tag="PKL11_04725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ12939.1"
                     /translation="MAKEIKINDKVFKQKVANKHHQDRVAREHQQRRWLLIGVAVVAV
                     LVVGLVAAGLLQEYVFKPMKAVAIVDNEKITVSDFEARARFQRHAIITSALQYAQIYQ
                     QFGMDPNAILEDADANLAAETLGNDTLDAMINEIVIEKEAIARGITVSDEEVEIALQE
                     AFGFYANGTPVPTATATVFPTLTVGAQQLTIVAYTATPTTAPTATATAIPETPVPTAT
                     ATATAVPPTATPEPTATPEPTAGPSATPLPTATAYTLDVFEDNYTNYIDEIKAFDFPE
                     KDLRDMYRAKLYRDKMFELLTADVQPIQDQVWARHILVASQEEAQAVYDLLLAGGDWS
                     ELAMTYSSDTSNNTNGGDLGWFTRGAMVKPFEEAAFSQEVGTISQPVESEFGWHLIQV
                     VGHEERPLTATELTQYREVVFGQWLEDVKTGLKIEKFPDVWMPAVPTTPTWTDYQ"
     gene            complement(3855..7466)
                     /gene="smc"
                     /locus_tag="PKL11_04730"
     CDS             complement(3855..7466)
                     /gene="smc"
                     /locus_tag="PKL11_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNZ12940.1"
                     /translation="MTLRLKSLELHGYKTFASRVVFEFPGNIAAIVGPNGSGKSNIAD
                     SLRWVLGEQSFSLLRGRKTEDMIFAGSEQRPRAGMAQATITFNNEDGWLPIDYSEVSI
                     GRRAFRDGDNEYVLNGQRVRLKEIGELLGQSGLSERTYTIIGQGLVDTALSLRPEERR
                     RFFEEAAGIGLYRARREESLNRLETTRRNLERVLDILSELEPRLKSLERQAQRAQEYE
                     RIKADLRLLLRDWYGYHWHTSQKELAHAQDIVRVHELKVEQARDKLAGVEIELKTLRE
                     QLNQLRAELNEWHSQSADLHNQWEKISKTLAVLDERERALSDQQAALQTDLATREEEQ
                     NARQQQAAASTEELTRLQAEQAEAQSQVEAARQQLAARQQERAGIEESLRNARRQMVQ
                     AETRQVQLKAHQAELETRLESLNKNQSNLNTQAEQTAAAFKQAEERRAAAEAQRAAAE
                     TALRQAEEALQTGQRRLAELEAERKDALDVRNALEGQRSRQRAQLDVLEQAEASFSGL
                     NEGSRRVLEAARQGHLKGLYQALGQIMEVPANLETAIAAALGEALDGVLLETQTDPEE
                     VLTLLEAGPKGRAVLLPVDWVRPTALEISSSDDCLGIAANLVKSSADYSPVVDLLLGQ
                     VVIVKDRAAARRMVADLPATGRVVTLKGEVFTASGLIIAGQEARSGMVGRSRQKRELQ
                     EALESVERQFNEHQAELRRLEAQLERERADRTEREKTLKAASAQVSAANQSLQQAQLA
                     VEQARQRGEWLRGQLKDLETQVQRAQTELQRSADEVKAAITQAESYNQQVRAAGHKLD
                     ALPLDEFQAQVVHWNTTAAVAERAARDAARRVEEYQQALSANARQQQEIRQRLSDTAE
                     QMARLQTEKITLHTAENELDEQIQAARRKIEPAEQRLRSLETQTAAVQDNYTLCQQAV
                     SVADRHATQSQLELSRQREALESLQRRIEDDFGLVSFEYTPTVSGPTPLPLDGMVEQL
                     PHVEQLAPEIEESINRQRSQLRRMGAINPDALTEYRSVKERLEFLTGQVEDLRRADAD
                     LREVIAELDTLMKKEFRRTFDAVAAEFRQMFTRLFGGGSARLMMTDEDNPTETGIDIE
                     ARLPGRREQGLSLLSGGERSLTAVALIFSLLKISPTPFCVLDEVDAMLDEANVGRFCE
                     LLRELSQTTQFVVITHNRTTVQTADVIYGITMGRDSASQMISLRLDEVSDDMIRRAD"
     gene            complement(7463..8185)
                     /gene="rnc"
                     /locus_tag="PKL11_04735"
     CDS             complement(7463..8185)
                     /gene="rnc"
                     /locus_tag="PKL11_04735"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNZ12941.1"
                     /translation="MERPEFEGEHEAPLTFAHRVGLQFTDWLLLSRALTHRSYLNEHP
                     EALEDNERLEFLGDAVLDFMVGAWLYNEYPEMPEGDLTRMRSALVYTEQLAVFARELD
                     LGLALRLGRGEMQGGGRDRDPLLCDAFEALIGALYLDGGIPAVERFMAPMLTRAADDI
                     LINRKNEDPKSRLQEWAQAQGYATPHYETTSVTGPDHSKVFEVSVLIRGEVWATGTGH
                     SKQAAAKEAARLALEKIGQDIT"
     gene            complement(8271..>9041)
                     /locus_tag="PKL11_04740"
     CDS             complement(8271..>9041)
                     /locus_tag="PKL11_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ12942.1"
                     /translation="ARGAQRAVEDSSGNAGASFAAYAAQAGLPARVYVPESASGPKRD
                     QIERYGAELIAVPGPRSAAAQAVLMEAEQGVPYASHAYLPYGLPGIATIAYELWESLG
                     RQAPGSVIAPVGHGGLLLGLVRGFAALLQSGSIARLPYFVGVQAAACAPLALAARQGA
                     AALENAPEGATIAEGVRVRRPVRGEALLAEIPPTQGCFVAVDEPRILPAFDQLARRGV
                     FVEPTSALAWAALLDNLEKLPAPIIVILTGLGLKYRPH"
     assembly_gap    9042..9141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9142..9526)
                     /locus_tag="PKL11_04745"
     CDS             complement(<9142..9526)
                     /locus_tag="PKL11_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ12943.1"
                     /translation="MPTIRCTLCQTPYPPQGFPYRCPECGGTLDFDAPPQFDPACIDP
                     SLPGLWRYRHTFDLSADAPLVTLGEGSTPLLWQSVQGHSVGLKLESLNPTGSYKDRGS
                     AVLVSQLLARGAQRAVEDSSGNAGAS"
     gene            complement(9530..10240)
                     /locus_tag="PKL11_04750"
     CDS             complement(9530..10240)
                     /locus_tag="PKL11_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNZ12944.1"
                     /translation="MSDLPFTILFFAHFKELAGAPQVEIHLPPNATVADLKARLASII
                     PALVNALPTAVVAINQEFGFDEDLIPAGAEIALFPPVSGGSGAVIQAQITTEPLDLNQ
                     LTAQHVDAETGAVCLFVGVVRGETHRGAAHTTASLEYEAYTAMAEAKLRQIGAEIQSR
                     WPQVLQIALVQRIGILEPGAPSVAVICTAAHRDEGVFEAARYGIDRLKEIAPIWKKEI
                     GPNGETWVDGDYHPQPGD"
     gene            complement(10233..10715)
                     /gene="moaC"
                     /locus_tag="PKL11_04755"
     CDS             complement(10233..10715)
                     /gene="moaC"
                     /locus_tag="PKL11_04755"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNZ12945.1"
                     /translation="MSEKHLSHLDEHGRAHMVAVGAKPDTERVAIARGEVHLQPDTVA
                     LIRQGLMKKGDVLTVAQIAGIQAAKRTADLIPLCHPIPLHQIAVDLALDDTLPGVVIT
                     ATVRTVGKTGVEMEALTAVSVAALTIYDMVKAVEKTGRIQNIRLLEKHGGLSGDIVNE
                     "
     gene            10836..11159
                     /locus_tag="PKL11_04760"
     CDS             10836..11159
                     /locus_tag="PKL11_04760"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNZ12946.1"
                     /translation="MMLEPQDAQIARLLKQRIQTISHIEKMIVFGSRARGDADAESDM
                     DVFIEVPILTPSIHQQILETAWEIGLDNGLVISPFLASTSLLTNGPLAGNPILRAIQS
                     EGVAV"
     gene            11156..11563
                     /locus_tag="PKL11_04765"
     CDS             11156..11563
                     /locus_tag="PKL11_04765"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNZ12947.1"
                     /translation="MTPTPIEQLIEMRLEQAHESLREANTLREAGLLRGTINRAYYAM
                     FYAALALTALRQEITSKHSGVVAFFDRNFIKTGIFPRELSRALHLAFQRRQETDYGDV
                     FTVNEEEALQSFIEADRFVAAVEAYIRSNKDSE"
     gene            11587..12378
                     /locus_tag="PKL11_04770"
     CDS             11587..12378
                     /locus_tag="PKL11_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNZ12948.1"
                     /translation="MSFQVATHQAEGYTVETEVYQGPLDLLLELIERAELDITHLALA
                     QVTDQYLAYLKQLEERNAAEVSAFLVIAARLIQIKSAALLPRPALPSVRGDEEDPAEA
                     LARQLILYKRFKELAGELEARQNAGWRTYLRVAAPPKMAVTARLDLTGVTIEDLMQAA
                     RDIFAHPGRLPELSQVVNMPRITIRERIKVVLDGLRQLGSTSFRKLISPQASRTEIVV
                     TFLAMLELIKQHIIQANQNALFSDIDLQAEGSLEDKENVEVEFVD"
     gene            complement(12384..12929)
                     /locus_tag="PKL11_04775"
     CDS             complement(12384..12929)
                     /locus_tag="PKL11_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12949.1"
                     /translation="MDSQVKFLPFHAINDFMLDEFRLEVIRTALGGLDNLPAERRSAI
                     NNQIKRLFKIPGFRNSAAAPIALKIRNAVPVFEESPVFAAEVLAAWTDLHSELANQVH
                     AFLTERGWPLLPLDAPRTRLPGFLTTWPRAEEFEVLVKAFREQHPDTPASDDDISLMV
                     AWLSGRLPLDLVDAAFETPAE"
     gene            complement(12963..13499)
                     /locus_tag="PKL11_04780"
     CDS             complement(12963..13499)
                     /locus_tag="PKL11_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12950.1"
                     /translation="MKNLPSIKWLSLLVILLTLVGCTGGMGGMQAEFAEMPVGMGYAN
                     GEKIYFMHTEASDPEIAQALSEMMDSPVLTVPALAQVPLAALANVYVFKNGVEGSGPL
                     GYQADVFDAPPGDPGYSPLRRIVWVTYEQWADSPQTAHLLYSVDEIFAAEASGLLTLE
                     PSNIVVNMPFVTWDGDQR"
     gene            complement(13511..15943)
                     /locus_tag="PKL11_04785"
     CDS             complement(13511..15943)
                     /locus_tag="PKL11_04785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNZ12951.1"
                     /translation="MTEPQHVTLPITGMTCANCVATVERSLKKVNGVQSATVNLSSER
                     ATVEFDPSLAGLDDLIARVERAGYGIAAGEADLIIQRLSDDNDARRLQKTLSALPGVL
                     EAAVNFASGRAHVRYLPTMVSQMDLRRAIRSAGFDALELGGEAEDAEAKARTAEIASQ
                     RHYLIIGLIFTLPLFLFSMGRDLGLLPAWAHQPWANWLMLALALPVQFYVGRQYYVSA
                     YKALRNGAANMDVLVAMGSSAAFLYSLPVTFGWIEGHVYYETAAVIITLIKLGKYLEA
                     RARGGTSEAIKKLMGLRARTAQILRDGVEQQVPVDEVRVGDVVLVRPGEKIPVDGVVL
                     EGRSSVDESMLTGESLPVEKGPGAPVIGATLNKQGLLRFEATRVGRETALAQIIRLVE
                     EAQGSKAPIQKLADQISAVFVPVVIGIATLTFLIWFFLVPIAVNADVSLFTRALIKMV
                     AVLVIACPCAMGLATPTAVMVGTGKGAELGVLIKNSEALERAGKLSVVVLDKTGTITR
                     GQPAVTDIVHSFPAGENELLRLAASVEKGSEHPLGEALVAEAGMRDLPLSDPLAFKAE
                     AGHGVQAQVEGHTVMVGSPRMMERYQQDLNGLSAAVDRLQQEAKTAILVAVDGVVQGV
                     FGIADTIKDGSLEAIDRLHALGLKVIMLTGDNRQTAAAIARQVNVDGVLAEVLPGDKA
                     AEVKRLQADGAVVAMVGDGVNDAPALAQSDVGIAIGTGTDVAMASAPVVLMSGDLRGV
                     PRAIALSRRTLRTIRQNLFWAFFYNVVLIPAAALGLLNPMLAAAAMAFSSIFVVTNSL
                     RLRKWQDRSTTS"
BASE COUNT         3531 a         4184 c         4953 g         3272 t
ORIGIN      
        1 tggggcaacg aacgcgattt tgcctgatgg caatccggtg atgccagcca ttccctttgg
       61 cacagagtct ataaaaacct ggtgttatta ttatgaaaag gcagaactgg ccctccagga
      121 agaagactgg cggactgctt atgatatgtt tactgaagga ttgggccaag gattcagccc
      181 cagcgatggg atagaatggc tgacgctggc acaatcggcc ctggtgcttg gagatgaagc
      241 cgggcttacc gccgcggcag aaggggtgga aagcgacccg tttgcccgct ggcaagcctg
      301 cactgcgatt caccggatgg aggaggaagg gttgtccatc gatgcggcaa atctcacact
      361 cagtgagcag ctattctgcc aaccttagcg aggctagctg ttcgatttgt tgggcagctg
      421 cgggatgagc caatccagga aggcgcgctt gttgcgggtt tgcagcagaa cgcggccggg
      481 gccggtcagg tctaccacca ggccttcacc tgaaagcagc gtggatttga tgccgccgag
      541 ggtgcggatt tcatacttca tggcacgctc aaaggcaacc aggtgaccgg tatcgatggt
      601 gtaggtctgc ccggcagcaa ggtcgataga atggatggcg ccatagctgg acaagatcag
      661 ttcaccggca ccactgcagc gcagcataat gaggccttcg ccgccaaaga aagtgcgggc
      721 accggtccat ttggtatcag ttgtgactgt ttcggcggag gcgacaaagg aacccgactg
      781 aacaagaaat tcctggtttt gaaggttcag aacggtgacg tcaccgggga gggtgggggc
      841 aagtagaatt tcatcaccag cagcggcggc gcgataggtg ttggtgaaaa aggattcgcc
      901 gcccagggcg gcgcgcgcca gggactgcat caaaccgccg cgcatgcggg tttcaacctg
      961 aatgccgcgc gacatgccca ccatggcgcc gggttcagca aggatggctt caccggtgtt
     1021 caggcggacc tgggcaacgg aataagaggg gcggtagagg atttcaattt gcatggggga
     1081 tctcctttga agagcagcga tatggctacg gtgaaattag aactcatcag acagaagtga
     1141 aatttgtcta tttttgtgaa atctattgta atccatcact ttgattttta gaacaatgat
     1201 taggtaaatt gagggatata tgttataatc gggcttcgat aagtcggcag ccaatggctg
     1261 ctgcctttcc gcctctggca gcatccacga tgttgaccgg gggcaatccc agggaaagga
     1321 ggtttccata caatgcgtaa ctatgaggtg atcgtcatcc tcaatcctga actcgaagaa
     1381 agcgctgtga ctgcgctgat tgagaaaatc aagggttgga ttgtcgaatc cggcggtgcg
     1441 gtttctcagg tggacatgtg gggtaaacgc cgcatggcct atctcatccg caagcaacgc
     1501 gaaggccagt atgtgctgct gcatgcacag tttgcgcctg cattcgcggc cgagctggaa
     1561 cgcaacttgc ggttccagga agtaattttg cgcttcctca tctcgaatgt tgagtaggcc
     1621 agtctcaaag acgtttgtat cagacggtga ttgcaaggag attgtacgat gagccgagga
     1681 ctgaacaaag tgatgttgat tgggcatctg gggcgcgacc ccgaaatgcg ctatacgcca
     1741 tcgggcaagc cggtgaccac gttcagcgtg gcgaccagcc gctcgtggag cactgcagac
     1801 ggtgaacgta agaccgagac ggagtggttc aacatcgttg cgtggggcaa cctggcagaa
     1861 atctgcaagc aatacctgac caaaggccag caagtttaca ttgagggccg gctgcaaacc
     1921 cgccgctggg aagacgctga aggcgtgaaa cattccagcg tggaggttgt ggcggctgag
     1981 atgatgatgc tgggcgaacg ccgggataca aacgctggca ccacgtctac cgatgaagtc
     2041 gctgacgaca tcgaagacga atatccattt taggagtaag tccaatgtct gaagaaaata
     2101 tggttgaagg cgaacagatg atgggtggcc gccgctatat ggcccgcccc aaaatctgcc
     2161 aattttgcgc cgataaaaac ctgaaaattg actataaagc gctggatatg ctgcgccggt
     2221 tcgttaccga agaaggtaag atccgccccc gccgccaaac cggcacctgc gcccgccatc
     2281 aacgcgaact ggctggcgcc gtgaaacgcg cccgccatat tgcgctgctt ccctacgtgg
     2341 aagaccgcaa cggctaagcc tggcgagaca caccaacaca ctctgcaggg gcaacgggtc
     2401 atccgcccat tgcccctcat tttatgatga aaggccagta tggctaaaga aatcaaaatt
     2461 aacgacaagg tcttcaaaca aaaagtcgcc aataaacatc atcaagatcg tgtggcgcga
     2521 gagcaccaac agcgccgctg gctgctgatt ggcgtggcgg ttgtggctgt gctcgtggtc
     2581 ggactggttg ccgccggatt gctgcaggaa tacgtcttca aaccgatgaa ggcagtggca
     2641 attgttgata acgaaaaaat cacggtctct gatttcgagg cccgggctcg cttccagcga
     2701 catgctatta ttacctctgc gcttcagtat gcccaaatct atcagcagtt tggcatggac
     2761 ccgaacgcaa ttcttgagga tgccgatgcc aacctggctg ctgaaacact gggcaatgat
     2821 acgttggatg ctatgatcaa tgagattgtg attgaaaaag aagccattgc tcgcggcatc
     2881 actgtcagtg atgaagaagt cgaaatcgcc ctgcaagaag cttttgggtt ctatgccaat
     2941 gggacgcctg tgcccacggc tacggcgaca gtcttcccga cgctgacggt tggggcgcag
     3001 cagttgacga ttgtggctta tacggccacg ccgaccacag cgccgaccgc cacagctacg
     3061 gcaatacctg agacgcccgt gcccaccgcg acggctacgg caacggctgt gccgcctacg
     3121 gcgacacctg agcccacggc gacccccgag ccaaccgccg ggcccagcgc cacgcccttg
     3181 cccacggcaa ctgcatacac gctggatgtg tttgaagata attacacaaa ctacatagac
     3241 gaaatcaaag cttttgattt cccggaaaaa gacctgcgcg atatgtaccg cgccaagtta
     3301 tatcgggata agatgtttga actgctgact gccgatgtgc agcctattca ggatcaggtt
     3361 tgggcacggc atattctggt tgcaagccag gaagaagcac aggctgtgta cgacctgctg
     3421 ctggctggcg gtgactggag cgagctggca atgacctact cgagcgacac ctccaataac
     3481 accaatggcg gcgacctggg ctggttcaca cgcggtgcga tggtcaagcc gtttgaagaa
     3541 gctgcattca gccaggaagt tggcaccatc agccaaccgg ttgagtccga atttggctgg
     3601 cacctgattc aggttgtggg ccacgaagaa cggcctctga cggcaacgga actgacccaa
     3661 taccgcgaag ttgttttcgg tcaatggctg gaagacgtta aaactggcct gaagattgaa
     3721 aaattcccgg atgtctggat gccggcagta cccaccaccc ccacctggac agattatcag
     3781 tagattgcaa gtaaagaacg tcaaacgggc ggcataagcc gcccgttttt tgttaattta
     3841 tccaaccggt ctcattaatc tgcgcgccgg atcatgtcgt cggagacttc atccaggcgc
     3901 aggctgatca tttgactggc ggagtcgcgg cccatggtga tgccgtagat cacgtcggca
     3961 gtctggacgg tggtgcggtt atgggtgatg acgacgaact gggtggtttg cgataattca
     4021 cggagcagtt cgcagaagcg gccgacattg gcctcgtcga gcatggcgtc cacttcatct
     4081 agcacgcaga agggcgtagg cgaaattttc agcagtgaga agatcagggc gacagcggtg
     4141 aggctgcgct cgccgccgga aaggagggac aggccctgct cacggcgccc gggcaggcgg
     4201 gcttcgatat caataccggt ctcggtgggg ttatcttcat cggtcatcat caggcgggcg
     4261 gagccgccgc cgaacaggcg ggtgaacatc tggcggaatt cagccgcaac cgcgtcaaag
     4321 gtgcggcgga attctttttt catcagggta tccagctcgg caatgacttc acgcaaatca
     4381 gcatcggcgc ggcgcaggtc ttccacctgg ccggtcaaaa attcgaggcg ttctttgaca
     4441 gagcggtatt ctgtgagggc gtcgggattg atggcgccca tgcggcgaag ttgggagcgc
     4501 tggcggttga tgctttcttc gatttcaggg gctaactgct cgacatgggg caattgctca
     4561 accataccat caaggggcag cggcgtaggg cccgagactg tgggagtgta ttcaaatgag
     4621 accaggccga aatcatcctc gatccggcgc tggagcgatt ccagggcttc acgctggcgg
     4681 ctgagttcca attgggactg ggtggcgtgg cggtcggcaa cactgacggc ctgctggcag
     4741 agggtgtaat tatcctgcac agcggcggtc tgggtttcaa ggctgcgcaa gcgctgttcg
     4801 gcgggttcaa tcttgcggcg ggcggcctga atttgctcat cgagttcgtt ctcagctgtg
     4861 tgcaaggtga ttttttcggt ctgcaaacgg gccatttgtt ccgctgtgtc actcaaacgc
     4921 tggcggattt cttgctgctg gcgggcgttg gctgaaagcg cctgttgata ttcttctacc
     4981 cgacgggcag catcccgggc agcgcgctca gccacggcgg cagtagtgtt ccaatggaca
     5041 acctgggcct ggaattcgtc caggggcaaa gcatccagct tatgaccggc agcgcgtacc
     5101 tgctggttat aggattcggc ctgggtaatg gcggctttga cttcatcggc cgagcgctgt
     5161 aattcggtct gggcgcgttg gacctgtgtt tcgaggtctt tcaactggcc gcgcagccat
     5221 tcgccgcgct gacgggcctg ctcaacagcc aactgggcct gttgaaggga ttggttggcg
     5281 gcactgactt gagccgaggc tgctttgagc gttttctcgc gctctgtgcg gtcagcgcgc
     5341 tcgcgctcca attgagcttc aaggcggcgt agttccgcct gatgttcgtt aaattgacgt
     5401 tcaacggatt cgagggcttc ctgcagctca cgcttctggc ggctgcgacc taccatgcca
     5461 gaacgggctt cctgcccggc aataatcaga ccagaggcgg tgaagacctc acccttgagg
     5521 gtgaccacgc gaccggtggc gggcaaatcg gctaccatgc ggcgggcggc ggcacggtct
     5581 ttgacaatga caacctgccc gagcaaaaga tcaaccaccg ggctgtaatc ggctgaggat
     5641 ttcaccagat tggcagcaat ccccaggcag tcatcagaag acgagatttc cagggcggtt
     5701 gggcggaccc aatccacagg caggaggaca gcgcggcctt tggggccggc ctccagcaga
     5761 gtcagcactt cttcggggtc ggtctgagtt tccagcagaa cgccatccag ggcttcacca
     5821 agggcggcgg caatggcggt ttccagatta gcggggactt ccatgatctg ccctaaagcc
     5881 tgatagagac ctttgaggtg gccctggcgg gcagcttcga gaactcggcg ggaaccttcg
     5941 ttcagaccac tgaaagaggc ttcggcctgt tcgagtacat ccaactgggc gcgctggcgg
     6001 gagcgctgac cttccagagc gttgcgcaca tcgagggcgt ctttgcgctc ggcttccagt
     6061 tctgccaggc ggcgctgacc ggtctgaagg gcttcttcgg cctgacgcag ggcggtttca
     6121 gcggcggcgc gctgcgcctc ggcagcggca cggcgttctt cagcctgttt gaaggcagca
     6181 gcggtttgtt cggcctgggt gttgaggttg gactggtttt tattcaggga ttccaggcgg
     6241 gtctcaagtt cggcttgatg ggctttcaac tgcacctggc gggtttcggc ctgaaccatc
     6301 tggcggcggg cattgcgcag ggattcttca attcctgccc gctcctgctg acgcgcagca
     6361 agctgctggc gggcggcctc gacctgcgac tgagcttcag cctgttcagc ctgaagacgg
     6421 gtcaattcct cggtagaggc tgcggcctgc tgctggcggg cgttctgttc ttcctcgcga
     6481 gtcgccagat cggtttggag cgcggcctgc tgatctgaaa gggcacgctc acgctcatcc
     6541 aaaacggcga gggttttact gattttttcc cactggttgt gcaggtcggc agactggctg
     6601 tgccattcat tgagttcggc acggagctgg ttaagctgct cgcgcagggt tttcagttca
     6661 atttcaacac cggcgagctt gtcgcgcgcc tgttcaacct tcaactcgtg cacgcgcaca
     6721 atatcctggg catgcgccag ttctttctgt gaggtgtgcc agtggtagcc ataccaatca
     6781 cgcagcaaca ggcgcaagtc ggctttgatg cgttcatatt cctgggcacg ctgggcctga
     6841 cgttcaaggc ttttgaggcg cggctccagc tcgctgagaa tatccaggac tcgctcgagg
     6901 ttgcggcggg ttgtttccag gcgattgagg gattcttcac gccgggcgcg gtagaggccg
     6961 atgccggcgg cctcttcaaa aaagcggcgg cgctcttccg ggcgcaggga gagggcggta
     7021 tcaaccagac cctgaccaat gatggtatag gttcgttctg acaggcccga ttggcccagg
     7081 agttcgccaa tttctttgag gcggacgcgc tgcccgttga gcacatactc gttatcgcca
     7141 tcgcggaagg cgcggcggcc aattgagact tcggaataat caatgggcag ccagccgtct
     7201 tcgttattga aggtgatggt agcctgcgcc ataccggcgc gggggcgctg ctcggaaccg
     7261 gcaaaaatca tgtcttcggt tttgcggccg cgcagcaggc tgaaagactg ctcacccaag
     7321 acccagcgga gggaatcagc aatattggac ttgcccgaac cgtttgggcc aacaatggct
     7381 gcaatgttgc cggggaattc aaacacaacc cggctggcaa aagttttata gccgtgcaat
     7441 tcgagagatt tgaggcgcag ggtcatgtga tatcctgccc aattttttcc agcgccaggc
     7501 gggcggcttc tttggcagcc gcctgcttgc tgtggccggt gccagtagcc cagacttcgc
     7561 cccgaatgag gacgctgacc tcaaacactt ttgaatggtc agggccggtg acgctggtgg
     7621 tttcgtagtg gggtgtggcg tagccctggg cctgggccca ttcctgcagg cggctcttgg
     7681 gatcctcgtt tttgcggttg atcaaaatat catcggcggc acgggtgagc atgggcgcca
     7741 tgaagcgctc cactgccgga atgccgccat ccagatacaa ggcgccgatc agcgcctcaa
     7801 aggcatcaca tagcagggga tcacgatcac gaccgccgcc ctgcatttcg ccgcggccca
     7861 ggcgcagcgc caggcccagg tccagttctc gggcaaagac cgccagctgc tcggtataga
     7921 cgagggcaga acgcatgcgg gtcaggtcgc cttcgggcat ttcggggtat tcgttgtaga
     7981 gccaggcgcc caccatgaaa tccagcactg catcgcccag aaattcgagg cgctcgttgt
     8041 cttcgagggc ttcgggatgt tcattaaggt aggagcggtg ggtaagcgct cgactgagca
     8101 ggagccagtc ggtaaattgc agacccacgc ggtgggcaaa ggtcaggggc gcttcgtgct
     8161 cgccctcaaa ttccggtcgt tccattggat cctccaagaa ctccgggagc ggcagacacc
     8221 tcaccgggat agtcaggtgg cttgcgttcc atgagttcaa atgacatgat ttagtgcggt
     8281 ctgtatttta accccaaacc ggtcaaaatc acaataatcg gggcaggcaa tttctccaga
     8341 ttgtcgagaa gggctgccca ggccaaggca gaagtgggtt cgacaaagac gccgcggcgg
     8401 gcaagttgat caaaagcagg aaggatgcgc ggttcgtcga cggcaacaaa gcagccctgg
     8461 gtgggaggaa tttcagccag aagggcctct ccgcgcaccg ggcggcggac gcggacacct
     8521 tcggcgatgg tcgcgccctc gggggcgttc tccaaagctg cggcaccctg ccgggctgcc
     8581 agggccagcg gagcgcaggc agccgcctgg acgccgacaa aataggggag ccgggcgatt
     8641 gaaccagact gcagcagggc agcaaagccg cggacaagcc ccagcagcag cccaccatgc
     8701 cccacagggg caatcaccga gccgggagcc tggcgtccca acgattccca cagctcatag
     8761 gcaattgtag cgatgccggg aaggccatag gggagatagg catggctggc atacggcaca
     8821 ccctgttcgg cttccatcag aacagcctgc gccgcagcgg agcgcgggcc aggaacagca
     8881 attagttcgg cgccatagcg ttcaatttga tcacgcttgg ggccggaggc ggattcgggg
     8941 acatagaccc gggcaggcag cccggcctga gcggcatagg cggcaaacga ggcgccggca
     9001 ttgccagatg aatcttccac ggcgcgctga gcgccgcggg cnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nacgaggcgc cggcattgcc agatgaatct tccacggcgc
     9181 gctgagcgcc gcgggcgagg agttggctga ccaacacggc gctgccgcgg tctttatagg
     9241 agccggttgg attgagcgat tcaagcttga ggcctactga gtggccctgc acagattgcc
     9301 agagcaaggg ggtgctgcct tctcccagag tgaccagagg ggcatcagcg gaaagatcaa
     9361 aggtatggcg gtaacgccac aggcccggca gagaagggtc aatgcaagcg ggatcaaact
     9421 gcgggggggc gtcaaagtca agggtgccgc cgcactccgg gcagcggtag ggaaagccct
     9481 ggggtgggta aggggtctgg caaagggtac agcgaatggt cggcatgggt taatctccag
     9541 gctgggggtg gtaatcacca tccacccagg tttcgccgtt ggggccaatt tcttttttcc
     9601 agatgggggc aatttctttc aggcgatcaa tgccgtaacg cgccgcttca aagacgccct
     9661 catcacggtg ggcggcagtg cagatgacgg caaccgaagg agcgccgggt tccaggatgc
     9721 caatccgctg gacaagggca atttgaagga cctgcggcca gcgggattgg atttcagcgc
     9781 caatctggcg gagcttggct tcagccatgg cggtgtaggc ttcgtattcc agtgaggcgg
     9841 tggtatgggc cgcgccgcga tgggtttcac cgcgcacaac accgacaaag aggcacaccg
     9901 cgccggtttc ggcatctaca tgttgggcgg tgagctggtt cagatccaag ggttcagtgg
     9961 tgatctgggc ctgaatgacc gcgcccgagc cgccgctgac aggcggaaag agggcaattt
    10021 cagcgcccgc ggggatgaga tcttcgtcaa agccaaattc ttgattgatg gcgactacgg
    10081 cggtgggcag cgcattgacc agggcgggga taatggaggc aagcctggct ttgagatcgg
    10141 caacggtggc atttgggggt aaatggatct cgacttgagg ggctccggca agctctttga
    10201 agtgggcaaa aaacaggatg gtgaagggca ggtcactcat tgacgatatc tccgcttaag
    10261 ccgccatgtt tttccagcag gcggatgttc tgaatgcggc ccgtcttttc gaccgctttg
    10321 accatatcat aaatggtcag ggcagcaact gaaaccgctg tcagggcttc catttccacg
    10381 ccggttttgc cgaccgtgcg gactgtggcg gtaatgacca ctccggggag cgtgtcatca
    10441 agggcgaggt ccacggcaat ctgatgcaag gggatgggat gacagagggg aatgaggtcg
    10501 gcagtgcgct tggcagcctg gatgccagca atctgggcca cggtgaggac atcgcctttt
    10561 ttcatcagcc cctggcgaat gagggcgacc gtgtcgggct gaaggtggac ttcgccgcgg
    10621 gcaatggcga cgcgctcggt atcgggcttg gcgccaacag cgaccatgtg agcgcgcccg
    10681 tgttcatcca gatgggagag atgtttttct gacatagagt aattataatg ggagatagat
    10741 aatcctgtac tcaggtcggg ttgaggtttt ggataagcgt taaccctgga tgcgtttttt
    10801 cttcgatttc ttgtataatg aatgcaggag cgtttatgat gctggaacct caagatgcac
    10861 aaattgcaag actgctaaaa caacggattc aaaccatttc tcatattgaa aagatgattg
    10921 tgtttggatc acgggcacgc ggtgatgcag acgccgaatc agacatggat gtttttattg
    10981 aggttccaat actcactccg agtatacacc aacagattct ggaaacagcg tgggaaattg
    11041 ggctggataa tggactggtg atatcaccat ttcttgcttc cacaagtctt ctaaccaatg
    11101 gaccgctggc tgggaacccc attctacgcg ctatccaatc cgaaggtgtt gctgtatgac
    11161 gccaacgcca attgagcagt tgattgagat gcggctggaa caggcccatg agagtttacg
    11221 agaagccaac acacttcgtg aagcgggttt gttgcgtgga acgataaacc gagcttatta
    11281 cgcaatgttt tatgcggcgt tggcattgac agcgcttcgg caagagatta cttccaagca
    11341 cagcggggtt gttgcttttt tcgatcgaaa ctttattaag acgggaatat tcccaaggga
    11401 gctttcaaga gcgctccatc tggctttcca gcgccgtcaa gaaaccgatt atggggatgt
    11461 gtttacagta aacgaagaag aagcgctcca atcttttata gaagcggatc gttttgttgc
    11521 cgcggttgaa gcttatattc ggtcaaacaa agattccgaa taaacatcta gggtataatt
    11581 gcggctgtga gttttcaggt tgccacccac caggcggaag gatacaccgt cgaaactgag
    11641 gtttaccagg ggccgctcga tctgctgttg gagctgattg agcgcgcaga gttggatatt
    11701 acacatttgg cactggcgca ggtgacggat caatacctgg cttatttgaa gcagcttgaa
    11761 gaacgcaatg cggcagaagt ttcggcgttt ctggtgattg cggcgcggtt gatccaaatc
    11821 aagtcggcgg cgttgctgcc ccgcccggcg ctgcccagtg tgcgcgggga tgaagaagac
    11881 ccggcagagg cgctggcgcg gcagttgatc ttgtataaac ggttcaaaga actggccgga
    11941 gaacttgagg cgcgtcaaaa tgcgggctgg cgaacttatc tgcgggtggc ggcgccgccc
    12001 aaaatggcag tgacggcccg gttggatttg accggggtga caattgaaga cctgatgcaa
    12061 gcggcacggg acatttttgc gcacccaggg cggctgccag agttaagtca ggtggtcaat
    12121 atgccgcgca ttaccatccg cgagcggatc aaggtggtgc tggatggact gaggcagctt
    12181 gggtcaacca gttttcgcaa gttgatttcg ccacaggcaa gccggactga gattgtggtg
    12241 acgtttctgg cgatgctgga attgattaaa cagcacatta tccaggctaa tcagaacgcg
    12301 ctgttcagtg atattgacct ccaggctgag ggcagcctgg aggataagga aaacgtcgaa
    12361 gtcgagtttg tggattaagc cgcttactca gcgggggttt caaaggccgc gtccaccagg
    12421 tcgaggggca aacgaccaga cagccaggcc accatcagcg aaatatcatc atcactggcg
    12481 ggggtgtcgg ggtgctgttc acggaaggct ttcaccaaga cttcaaattc ttcagcccga
    12541 ggccaggtgg tgaggaagcc cggcaaacgg gtgcgggggg catccaacgg gagaagcggc
    12601 caaccgcgct cggtgaggaa ggcatgaacc tgattggcca gttcggaatg caggtcagtc
    12661 caggcggcaa gcacctcggc ggcaaacact ggagattcct caaaaaccgg gacggcattg
    12721 cgaattttca gggcgattgg agcggctgca ctgttgcgaa aacccgggat tttgaacagg
    12781 cgtttgatct gattattgat ggcgctgcga cgctcggcgg gtaaattatc cagaccaccc
    12841 agggcggtgc ggataacctc caggcgaaac tcgtccagca tgaagtcatt aatggcgtga
    12901 aatgggagaa atttaacttg ggagtccata gggatgatta tagtccaaaa tgtgaggaga
    12961 tatcagcgtt gatcgccatc ccaggtgacg aaaggcatgt tcaccacgat attggagggt
    13021 tcgagagtca gcaagccaga ggcttcggcg gcgaagattt catctaccga atagagcagg
    13081 tgggcagttt ggggcgaatc agcccactgc tcataggtga cccagacaat gcggcgcagg
    13141 ggggaataac caggatcgcc gggcggggca tcaaagacat ccgcctgata gcccagcgga
    13201 ccgctgccct caacaccatt tttaaagaca tacacatttg ccagggccgc gaggggcact
    13261 tgagccagag caggcacggt gagaaccggc gaatccatca tctctgagag ggcctgggcg
    13321 atttcaggat cggaagcttc ggtgtgcata aaatagattt tttcgccgtt ggcatatccc
    13381 atcccaacgg gcatttcggc aaattcagcc tgcatgccac ccataccgcc ggtgcagcca
    13441 accagggtca gcaaaatgac cagaagagac agccatttga ttgaggggag atttttcatt
    13501 atccaactcc ttatgaagtg gttgagcgat cctgccattt gcgcaggcgg agactattgg
    13561 taacgacaaa gatactgctg aaggccatgg cggcggcagc cagcattgga ttaagcaaac
    13621 caagcgcggc agcagggatc agaactacat tgtagaaaaa ggcccagaaa agattctggc
    13681 ggatggttcg caatgtgcga cgcgagaggg caatggcacg cggcacacct cgcaggtcgc
    13741 cgctcattaa gacgacaggg gcagaggcca tggcaacatc ggtcccggtg ccgatggcga
    13801 tacctacatc tgattgagcc agcgccggag catcattgac gccatcgcct accatggcta
    13861 caacggcgcc atcggcctgc aagcgcttga cttcggcagc tttgtcgccg ggcagcactt
    13921 cagccagtac accatccaca ttcacctggc gagcaattgc ggcggcggtc tggcggttat
    13981 cgccggtgag cataatcact ttcagcccga gggcatggag gcggtcaatg gcttccaggg
    14041 agccatcttt gatggtatcg gcgatgccga aaacgccctg taccacacca tctacggcga
    14101 caagaattgc ggttttggcc tcctgttgaa ggcgatcaac ggcggctgaa agtccattca
    14161 aatcctgttg atagcgttcc atcatgcgag ggctgccgac catcaccgtg tgaccctcca
    14221 cctgagcctg gacaccgtgc ccggcctcgg ctttgaatgc cagcggatca ctgaggggca
    14281 aatcacgcat accggcttca gccacaaggg cttcacccag ggggtgttcg gaaccttttt
    14341 cgaccgaggc agcaaggcgc aagagttcgt tttcgccggc agggaagctg tgaacaatat
    14401 cggtcacggc aggttggccg cgggtgatgg tgccggtttt atccagcaca acaacgctca
    14461 atttaccggc acgttccaac gcttcactgt ttttaatcag gacacccagc tcggcacctt
    14521 tgccggtgcc taccattacg gcggtggggg ttgccagtcc catggcgcag gggcaggcga
    14581 tgaccagtac ggcgaccatt ttgatcaagg cacgggtgaa taggctgaca tcagcgttga
    14641 ccgcaatggg taccaggaag aaccagatga ggaaggtaag tgtggcaatg ccgatgacta
    14701 ccgggacaaa taccgccgaa atctggtcgg ccagtttttg gatgggagct ttgctgccct
    14761 gggcttcttc taccaggcgg atgatctgcg ccagagcggt ttcgcggcca acccgggtgg
    14821 cctcgaaacg gagcagaccc tgtttgttca gggtggcgcc aatgaccggg gcgccggggc
    14881 ctttttcaac gggcagcgat tcgccggtca gcattgattc gtccacagat gagcgaccct
    14941 ccaggaccac cccatcgacg ggaatttttt cgccggggcg caccaggacg acatcaccga
    15001 cgcggacctc atcaacgggc acctgctgct ccacgccatc ccgcaggatc tgagccgtgc
    15061 gggcccgcag acccatcagt tttttgatgg cttcgctggt gccgccgcgc gcgcgggctt
    15121 cgaggtattt gccaagcttg atcagggtga tgatgacggc agcggtttca taatagacgt
    15181 ggccttcaat ccagccaaag gtcacaggca aagaatataa aaaggctgcc gaggagccca
    15241 tggcaaccag gacatccata ttggcggcgc cattgcgcaa ggccttatag gcggagacat
    15301 aatactgacg acccacatag aactgcactg gcagcgcaag agccagcatc aaccagttgg
    15361 cccagggttg gtgcgcccag gcgggcagca agccaaggtc gcggcccatg gaaaagagga
    15421 acagcggaag cgtgaaaatc aggccaatga tcaggtaatg gcgttgagaa gcaatttcag
    15481 cggtgcgagc tttcgcctcg gcatcttcag cttcgccgcc cagttcaagc gcgtcaaagc
    15541 cggcggaacg aatggcgcgg cgcagatcca tctggctgac catggtgggg aggtagcgaa
    15601 catgggcgcg gccagaggcg aaattaacgg cagcctcgag tacgccgggg agggcactga
    15661 gggtcttctg caggcggcgg gcatcgttat catcgctgag gcgctggata atcaaatctg
    15721 cttcgccagc cgcgatgcca tagccggcac gctccacccg ggcgatcaga tcatccaggc
    15781 cagcaagaga tggatcaaac tctacggtgg cgcgctcaga agacaggttg acggttgccg
    15841 attgaacgcc gttgactttc ttgaggctgc gttcaacagt ggcgacacag ttggcacagg
    15901 tcattccggt gatgggcaag gtgacatgtt ggggttctgt catggtataa tctccttacc
    15961 ccccccaggg gggtggggta aataactgcc ctgattttat ccgatttttg agaaggagtc
    16021 aaaccacaac cactgattac
//
LOCUS       DAOPVS010000035        15937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3746_length_15937_cov_58.838433, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000035 DAOPVS010000000
VERSION     DAOPVS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3746_length_15937_cov_58.838433"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1308
                     /locus_tag="PKL11_04790"
     CDS             151..1308
                     /locus_tag="PKL11_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNZ12952.1"
                     /translation="MREVAVIGIGQTPIEEQWDKSLKELAGEAALAALHDAGLERVDS
                     IFVGNMMSGSANHQQHLGTQIADWVGMRFAEAAHIESACSSGAAAFRAALIAVSSGAV
                     DAALAVGAEKMSDSPSDEITSELATAADFDFEAEIGLSFVAINALAMRRYMFEHGWKK
                     EDFAPFSINAHANGAHNPYARFRSAITEQDYFKAAMVADPISVMDAAGMGDGAAAVVL
                     VPAEYLRAAPGARIIRVAASAAATDTIALASRKEPTWLSAAEKSSRAAYAQAGVEPKD
                     IDVFEAHDAFSIMAALSLEASGFAERGQGPRLALDGEIRPTGRVPIATRGGLKARGHP
                     VGATGMYQIVEIIQQLRGEATGSQVDGAQIGMAQNIGGSGSNIITHILRAG"
     gene            1396..2262
                     /locus_tag="PKL11_04795"
     CDS             1396..2262
                     /locus_tag="PKL11_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNZ12953.1"
                     /translation="MEMLSLEAIKEYRARTFRMTPGLRLGSLEEAVGFVNACGFTFFW
                     PNAERPLPSLWAAVAGDRPVPDEHDDPAHVTWDWKDKALGKRLWYYGRVLAQRNAMVS
                     LEMLPYFYALSPNFGDPENDYLEQYAAGSLTMEAKGVYEALLKNGAMDTLALRRAARL
                     SSPESDHRFNRALNQLQMEFKVLPVGTAEVGAWRYAFVYELTNRHFPDLVQQAGAISE
                     PEARQALLVQYFQIVGVARLADITRLFRWRIDETTRAVNKLMEGGKLRGGYTVEGQKG
                     EWIVNLGFRILD"
     gene            2289..>3915
                     /gene="recQ"
                     /locus_tag="PKL11_04800"
     CDS             2289..>3915
                     /gene="recQ"
                     /locus_tag="PKL11_04800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNZ12954.1"
                     /translation="MDNVMPAAVLKNTFGYDQFLPNQREVIENILARRDTLAVMPTGG
                     GKSLCYQIPALIFDGVTVVVSPLIALMKDQVEQLHAAGVPAVVLNSSLSPEVYGENMK
                     SIRQGRAKLLYMAPETLLTERIFDLLDSIQVNLLTIDEAHCISEWGHDFRPEYRQLVE
                     VRRRYPRAVCLALTATATPRVRQDILDSLNFTKANQFVASFDRPNLFIEVAPKENPEH
                     QTVRFLQDFKDQSGIIYCFSRKQVDELAAYLIQKGFSALPYHAGLEDQQRRRNQEAFI
                     RDDAQIIVATIAFGMGINKPNVRFVLHYDLPKSIEGYYQEIGRAGRDGLPAHCLLLYS
                     YGDAAKQRYFIDQKQGRERQVAMQHLDAMIRYAEDEIRCRRVPLLTYFGETNVPAQCD
                     NCNNCTSAPPRLDDITIPAQKFLSCVIRTGEKFGAKHVAAVLRGEESEQITKWQHHQL
                     STYGIGKDFSEKQWLMVARQLIQMGYLIREEEFYTLSVTDKARAALKKRSKIMGQFKE
                     AQERPPAAARKKVDLETNNALYALLRQKRKELAD"
     assembly_gap    3916..4015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4016..4615
                     /locus_tag="PKL11_04805"
     CDS             <4016..4615
                     /locus_tag="PKL11_04805"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNZ12955.1"
                     /translation="TNNALYALLRQKRKELADAAGVPPYVIFPDTTLIEMAAYYPQSM
                     ERLMDITGVGKVKAERYGEIFLGLIQTFCKNKNLPEKQKVTRRSKSDTGRRYVFVGEA
                     YNAGESVTALMKRYNVTAGTILDHLARFIAAGNRLRKSSDLRQFIKVSPELQQAALAA
                     FRSNGAAYLRPVYDQLRGQVDFEQLKILRLVYLIEAQST"
     gene            complement(4632..5642)
                     /locus_tag="PKL11_04810"
     CDS             complement(4632..5642)
                     /locus_tag="PKL11_04810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNZ12956.1"
                     /translation="MLEKPTIPDELIAACLRTEYNLPAASIDFLPLGADQNTAVYRVA
                     TPAQSYFLKLRRGLFDEIAVALPKYLFDQGITQIIPPLPTVSGSLWGSLQGYKTILAP
                     FVIGHNGYEVRLSDHHWLALGQALKRIHTAPVLPSYAAQIPRETYSPCCRETVKTFLQ
                     QIEVDQFTDPVAAQTAALLQANRPVIEMLLLRAETLALRLSACPAEWAICHADLHAGN
                     LLVGSDDSLYLVDWDTLMLAPKEHDLMFIGAGLLGDWYTPAEESERFYPAYGPTRLNQ
                     VALAYYRYERILQDIAAFCEQLLLTAAGGEDREQSLYYLESNFKPNATIAIACQADQL
                     LW"
     gene            5775..9266
                     /locus_tag="PKL11_04815"
     CDS             5775..9266
                     /locus_tag="PKL11_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ12957.1"
                     /translation="MFEFHVSRQARDRYQFDQTLFRFDGNVIFANFHGARLFAQKMNQ
                     KRDLAAYPEQAVKAGQINAMGMVDEILHLVVSLYRRQKNPTAISGALAWLEERLGTEQ
                     VNRMLVRFTAEFPPVVVYQQQMTVDAYLAGSTDGIPHREAALEELLMLWISNKNPALA
                     PYLELFDDSGLQSETVYARALKELHIYFETQPKHGPDNQNLIDMLRAPALANPYSLTD
                     QLEFIRTRWTEMLGRYLYRLLSSLDLIKEENKQSFMGPGPTVIPTYQQPGSGLAEFEG
                     EPERFSPDQDWMPRLVLIAKNTYVWLDQLSKQYGREMRHLDQIPDEELDRLAQQGFTG
                     LWLIGLWERSRASAHIKQLCGNPEAIASAYSLFDYRIADDLGGELAYNNLSERAGQRG
                     IRLASDMVPNHMAIDSPWVVEHPDWFVSLDYSPFPGYTFNGPDLSEDGRVSINIEDHY
                     FDRTDAAVVFKRYDHTNGQTRFIYHGNDGTSMPWNDTAQLNYLNPAVREAVIQTILAV
                     ARRFPIIRFDAAMTLAKKHYQRLWYPEPGSGGDIPSRADHGMTRDQFNAAMPIEFWRE
                     VVDRVAQEVPNTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRNEDNAGYRQLIKN
                     TLEFDPEILKRYVNFMNNPDERTAADQFGKGDKYFGICTVMATLPGLPMFGHGQVEGF
                     SEKYGMEFRRAYWDERIDDGLVERHRREIFPLLHRRWMFGEVANFLLYDFFTGGGVNE
                     DVYAFSNGQGDERALVIFHNKYAQTSGWIRMSAAYVVKGTGDQRNLVQKSLAQGLNLH
                     PAPNAYLVYRDLTRGMEYIRPSQEVAERGLYLELNAYETHVFADFREVQDDSYHSYRQ
                     LCQYLNGRGVPSVTEALRELVLMPVQQPFREIANPGYFNYLLDARLDLETEALPAGLL
                     DEAEQKAGNLLDGIQYMSGYTQNRAEVQAGIRRAVQSTLSLRRLDTLFSLSTAAGFQK
                     ALDYLETGLVDNRRWRVLFGWAFTHNLGRVAGGTDPDAQTQSWLDEWQLGKMLNDNAL
                     AMGSDEGTARRMNQTLRLLLSHQNWYSQLGALPVEKIVREWLNAPEIQQFIGVNRYKD
                     VLWFNHESFVELVWWMATAAVLKVAANPGGTAAELLEQTLGAYEIAQALLKAEENSGY
                     QVDKLLALLSEAEEAAE"
     gene            complement(9338..10561)
                     /locus_tag="PKL11_04820"
     CDS             complement(9338..10561)
                     /locus_tag="PKL11_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12958.1"
                     /translation="MYIKRHIEQALQKAASMFGAVLVTGARQVGKTTLLREVVRDVTY
                     VSLDDPVLRNQAVHQPATFFLDSPPPICIDEVQYAPGLFPYIKIQIDQTKQKGLFYLS
                     GSQQFHMMKNVSESLAGRVGILNLLGISLRERFGISNNYPFVPDAAYFEGRKNDAPPL
                     AYKGIWQTIFRGSMPELVSNSQADWQMYYATYVRTYIERDVRELSQVADELKFYQFMT
                     AVAAMNGQLLNMASLSRDVGVSQPTIERWLSVLQASSLIYLLQPYHNNILKRAIKSPK
                     LYFMDTGLAAYLGRWPNSEVLSVGASAGAFFESFVISEIYKSYTNAGILQPPLYFYRD
                     RDGAEIDLLIQSGDTLHPIEIKKHADPRPDDISAFGKLDNLSGLKRGSGGIVCMYDRL
                     ITLKDTDKIIPVWYL"
     gene            complement(10649..12061)
                     /locus_tag="PKL11_04825"
     CDS             complement(10649..12061)
                     /locus_tag="PKL11_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNZ12959.1"
                     /translation="MSTHGKIPAWVQDTVFYQIFPDRFANGDASINPANVQPWGDPPT
                     RHGFMGGDLRGVLHHLDYLQDLGINAIYFNPIFLAASNHRYNAFDYFQIDPKLGNLND
                     FHALVDAAHRAGIRIILDGVFNHCGRGFFAFSDLLENQAESPYRDWFHVKNFPVSAYG
                     SHKNLNYEAWWGYSSLPKFNTANPQVRKYIMNVSRYWIEQGADGWRLDVPNEIDDDTF
                     WEEFRYNVRSVNPEAYLLGEIWDGNPRWVSPGHFDGLMHYPLRTSIFEYLAGKLDGAG
                     FTGQVQEQMSRYSQKNVFAMYLLLGSHDTERVLTVLDGNLDKLRLAYLLLFSFPGAPA
                     VYYGDEVGVAGGKDPDCRRAFPWEESSWNHDLRTWLRQLIAHRRGSFALRRGEFVPLA
                     QGSHGACAFARRADGHQMITVANPSRYKAHLEIPASALNASEGQVFTSPIDRQQIAVT
                     DDALRLTLPPVSGNWFAVHS"
     gene            complement(12058..12771)
                     /locus_tag="PKL11_04830"
     CDS             complement(12058..12771)
                     /locus_tag="PKL11_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNZ12960.1"
                     /translation="MSFGDRSQHTRVNPMLLGPLERPALRWLAAHMPAWVHPDLLTAF
                     GFFGTVVIFVSYILTNHSPWFLWLASLGLIMNWFGDSLDGTLARYRNIERPKYGFFVD
                     HIVDALDEVLVFLGLGLSPYIDFKIAALALIGYMLMSVLVYVDTIVTGKFRISYAKLG
                     PTEMRVLIIITNAVIFFAGNPLVNLPWVKWTPTLYDLILAFGAILMFVGFILYSYIKI
                     SELAKLEPSERKARQEQNQ"
     gene            complement(12783..13718)
                     /locus_tag="PKL11_04835"
     CDS             complement(12783..13718)
                     /locus_tag="PKL11_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12961.1"
                     /translation="MTAERPRCPRLNLQRFSNSRFAVSLMLTLSQLMPRWVGQQLVGL
                     VARLIVCRRQRPMVQAIRANQWVLSGQKLTAPELDARACQVIQASGSALFDHYHNLRA
                     PRRARRMVQFTPHMLDVIAERQQGQHGAVFVASHTGSFDLAGMALVQAGLKFLVLSFP
                     QPPGGYQLQNELRRLSGIEIMPLNIEALQAARQRLQSGGTVLTGLDRPWPESTHHPRF
                     FGRPTALPVAYIPLALRNKAPVYFVYCLSQPKGKYLLDCSEPIWLESYPDRDTEIIAN
                     AEKILRLAEEVLQAHPEQWAMTYPLWPESLDQIPS"
     gene            complement(13715..14743)
                     /locus_tag="PKL11_04840"
     CDS             complement(13715..14743)
                     /locus_tag="PKL11_04840"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNZ12962.1"
                     /translation="MRFLCFGVGAIGTYIGGSLALNGQEVVFLERPEGAAAARQSGLR
                     LVLPGGERQIDAPIIVDSIEEALSRGPFDYAIVAIKSYDTPALIDMLRPHQAQLPPLI
                     CLQNGVENENHLRSLVGFSRVIPGTVTSAVGRLGVGQIVLERLRGVGLAYVHPHVLDL
                     TTVFNQAGLNAQIYNHPNAMKWSKLLTNLPANATSAILNWTPAQVFSHPGIYRLEVTQ
                     FREALSVMRAWPLPVCDLPGTPVRALAWAFGYLPPLVSQPLLKRGLTKGRGDKMPSFH
                     IDLYGGRTRSEVEYLNGAVARQAAAFKRFAPVNQSLTRILSALAAGDLPRETYANQPE
                     KLLAEISR"
     gene            complement(14786..15694)
                     /gene="fmt"
                     /locus_tag="PKL11_04845"
     CDS             complement(14786..15694)
                     /gene="fmt"
                     /locus_tag="PKL11_04845"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNZ12963.1"
                     /translation="MDVRIVFMGSPDFAVPVLRRLTEAYPVIGVITQPDRPAGRGQTL
                     ASPPVKILAQSLSLPVIQPNRLKEPEAQAQLAAWAPDLIVVAAFGQILRQNVLDLPPH
                     GCVNVHASLLPRWRGASPIQAAIRTGDAQTGVSIMRMDAGVDTGPVLAQRAIPILPED
                     TAGALSDRLAQLGADLLIETLPAYLNGKIIPQPQDDALAVYAPMIQKENGLLDFSRPA
                     VELERQVRAYQPWPGAYTFWADAVLKIQRASAHPADHLSPGQRGIVAGFPAIGTALGC
                     LLLLELQPAGKRPMPGDVFLRGARGW"
BASE COUNT         3705 a         3848 c         5004 g         3280 t
ORIGIN      
        1 tgatatacta ttgctatgca gttcgcttgg gtgcccccct caccccggcg gactgctgct
       61 ttaagggaaa atttgcaaag gttggaattt tgatagttcc tgacagcaag gggcgatata
      121 attgacttta ctgacgaaag gatgactgcc atgcgcgaag ttgctgtgat tggaattgga
      181 caaactccga ttgaggaaca gtgggataaa tcgttgaaag agctggcggg tgaggcggcg
      241 ctggccgcgc tgcacgatgc cgggctggag cgggtggaca gtatttttgt gggcaatatg
      301 atgtctggct cggccaacca ccagcagcac ctgggaacgc agattgctga ctgggtgggg
      361 atgcggtttg cagaggcggc gcatattgaa tcggcgtgca gttcgggggc ggcggctttt
      421 cgggcggcgc tgattgcagt ctcttcgggg gcggtggatg cggcgctggc agtgggggcc
      481 gaaaagatgt cggattcgcc cagcgatgaa attaccagcg aactggcaac cgcggcggat
      541 ttcgattttg aggcagagat tgggctgtcg tttgtggcca tcaacgcgct ggcgatgcgg
      601 cgctatatgt ttgagcacgg ctggaagaaa gaggattttg cgccattctc gattaatgcc
      661 catgctaacg gcgcacataa cccttatgca cgcttccgct cggcgattac cgagcaggat
      721 tattttaagg cggcaatggt ggctgacccg attagtgtga tggacgcagc cggcatggga
      781 gatggggcgg cggctgttgt gctggttcca gcagaatatc tgcgggcagc gccgggggcg
      841 cgaatcattc gggtggcggc ttcggcagcg gccacggata cgattgcgct ggcaagccgt
      901 aaagaaccca cctggctgag cgcggctgaa aaatcatccc gggcggcgta tgcgcaagcg
      961 ggggtagagc cgaaagatat tgatgttttt gaggcacacg atgcgttcag cattatggcg
     1021 gcgctttcac tggaggccag cgggttcgcc gaacgcgggc aggggccgcg gctggcgctg
     1081 gatggcgaaa tccgtcctac ggggcgggtg ccgattgcaa cgcggggcgg gctgaaggcg
     1141 cgtgggcatc cggtgggggc aacggggatg tatcagattg tggaaattat tcagcagcta
     1201 cgcggcgagg ccactggctc acaggtggat ggggcgcaga ttggcatggc gcaaaatatc
     1261 ggcggcagcg gatcgaatat catcacgcat attctgcggg cgggttaagc cgcccgagat
     1321 atgaggttgg aagatcaagg ggcaggtgct gaggcagact gccgttttgg gctggattgg
     1381 attagaatag ccaacatgga aatgctgtcg ctggaagcga tcaaagaata tcgggcgcgg
     1441 acgtttcgga tgacgccagg gctgcgcctg ggttcattgg aagaggcggt agggtttgtg
     1501 aatgcctgcg gctttacttt cttttggccg aacgcagaac gccccctgcc gagcctgtgg
     1561 gcggcagtgg cgggtgaccg cccggtgcca gatgaacatg atgatccggc gcatgtcacc
     1621 tgggattgga aggataaggc gctgggaaag cggttgtggt attacggccg ggtgctggca
     1681 cagcgcaatg cgatggtttc gctggagatg ctgccctatt tttatgcgct ctcgccgaat
     1741 tttggcgacc cggagaacga ttatctggag cagtatgcgg cgggcagcct gacgatggaa
     1801 gccaaggggg tgtatgaggc tttgctgaaa aacggcgcca tggatactct ggcgctgcgg
     1861 cgggcggcgc ggctctcgtc accggaaagc gatcaccgct ttaaccgggc actgaaccag
     1921 ctgcagatgg agttcaaggt gctgccggtt ggaactgccg aggtcggtgc gtggcggtat
     1981 gcgtttgttt atgaattgac caaccggcat tttccggatc tggtgcagca ggcgggagcg
     2041 atatccgaac cggaggcgcg gcaagcgctg ctggtgcagt attttcagat agtgggggtg
     2101 gcgcggctgg ctgacattac tcgattgttc cgctggcgga ttgatgaaac cacgcgggca
     2161 gtgaataagc tgatggaagg tggaaaactg cgcggtggtt atacagttga aggacagaag
     2221 ggggagtgga ttgttaattt aggatttagg attttagatt gagattttgg tgattttgga
     2281 attgtttaat ggataatgtg atgccggcgg cagtgctgaa gaatactttt gggtatgatc
     2341 aatttctccc caatcaaagg gaggtgattg aaaatatctt ggcgcggcgc gatacgctgg
     2401 cagtgatgcc cacggggggc ggcaagtcgt tatgctatca gattccggcg ctcatttttg
     2461 atggggtgac ggtggtggta tcgccgttga ttgcgttgat gaaagaccag gtggagcaac
     2521 tgcacgcggc cggtgtgcca gcggtggtgc ttaacagctc gctgtcacct gaagtttatg
     2581 gcgagaatat gaaaagcatc cggcagggca gggcaaagct gctgtatatg gcccccgaaa
     2641 cgctgctgac cgaacggatt tttgacttgt tggattccat ccaggttaat ttgctaacca
     2701 ttgacgaggc gcactgtatt tcagagtggg ggcatgattt tcgaccagag tatcgtcaac
     2761 tggttgaggt acggcggcgc taccccaggg ccgtgtgcct ggcgctgacc gccaccgcca
     2821 cgccgcgggt gcggcaggac attctggaca gcttaaattt cacaaaggcc aatcaatttg
     2881 tggccagctt cgaccgtcct aacctgttta tcgaagttgc gcccaaggaa aacccggagc
     2941 accagaccgt gcggttcctg caagatttca aagatcagtc ggggatcatc tattgtttct
     3001 cgcgcaaaca ggtggatgaa ctggcggcgt atttgattca aaaagggttt tcggcactgc
     3061 cttaccacgc cgggctggaa gatcaacaac gccgccgcaa ccaggaagct tttatccggg
     3121 atgatgcgca gatcatcgtg gcaacgattg cctttggaat gggcattaac aaacccaatg
     3181 tgcgctttgt gctgcattat gatttgccca aaagcattga aggctattat caggagattg
     3241 ggcgcgccgg gcgggatgga cttccagccc actgcctgct gttgtacagt tacggcgacg
     3301 cggccaagca acggtatttt attgaccaga aacagggacg ggaacgccag gtggccatgc
     3361 agcatctgga tgccatgatc cggtatgccg aagatgagat ccgctgccgg cgtgtgccgc
     3421 tgctgacgta ttttggcgag accaatgtgc cggctcaatg cgacaactgc aataactgca
     3481 cctctgcccc gccccggttg gatgacatca ccatcccggc gcagaaattc ctctcgtgcg
     3541 tgatacgcac cggcgagaag tttggcgcca agcacgtggc ggcggtgctg cggggagaag
     3601 aatcggagca gattacgaaa tggcagcacc atcagctttc aacctatggg attggcaagg
     3661 atttcagtga aaagcagtgg ttgatggttg cccggcagct aattcagatg ggatatttga
     3721 tccgggaaga ggaattttac accctcagcg tgacggataa agcccgggct gcactgaaaa
     3781 agcgcagcaa gatcatggga cagttcaaag aggcgcagga aaggccgccg gcagcagcgc
     3841 gcaaaaaggt tgatctggaa accaacaacg cgttgtatgc gctgctgcgg cagaagcgca
     3901 aagaacttgc cgatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccaa
     4021 caacgcgttg tatgcgctgc tgcggcagaa gcgcaaagaa cttgccgatg cggcgggtgt
     4081 gccgccttat gtgatctttc cagatacaac cctaattgaa atggcggctt actatcccca
     4141 atcaatggag cggctgatgg atattaccgg ggttgggaag gtgaaagccg aacgctacgg
     4201 cgagatattc cttggcttga tacagacttt ttgcaaaaac aagaatctgc ctgaaaagca
     4261 gaaagtaacc cggcgcagca aaagcgacac ggggcggcgg tatgtatttg tgggcgaagc
     4321 ctataatgcc ggtgagagcg tgactgccct gatgaagcgt tataacgtta cggctggcac
     4381 gattcttgac cacctggcac ggtttatcgc ggcaggaaac aggctgcgta aaagcagtga
     4441 tctgcgtcaa tttatcaaag tatcgcccga attgcaacaa gccgccctgg cagctttcag
     4501 atccaatggg gccgcgtacc tgcgacctgt atatgatcaa ctacggggac aggttgattt
     4561 tgagcagcta aaaattttgc gactggttta tttgatcgaa gcccaatcca cctaaccgtg
     4621 agggcggatg attaccagag cagttgatcg gcctggcagg caatggcaat ggtggcgttg
     4681 ggtttgaaat tcgattccag ataatacaag gactgttcac gatcctcgcc gccggcagcg
     4741 gtgagcagga gctgctcgca gaaggcggca atgtcctgga ggatgcgctc gtagcggtag
     4801 taggcgaggg ctacctggtt caaccgggta gggccatagg cggggtagaa gcgctccgac
     4861 tcttctgccg gggtgtacca atcccccaac agacctgcgc caatgaacat caaatcatgc
     4921 tctttggggg ccaacatcag ggtatcccaa tcgacaagat agagggaatc atcgctgcca
     4981 acgagaaggt tgccggcatg cagatcggca tggcagattg cccattctgc cgggcaggca
     5041 gacagcctga gcgccagggt ttcggcccgc agcagcagca tttcaatgac cgggcggttt
     5101 gcttgcagca aagctgcggt ttgtgcagcc accggatctg taaattgatc cacctcaatc
     5161 tgctggagaa aggttttaac cgtttcacgg cagcagggag aatacgtctc acgggggatt
     5221 tgagcggcgt atgaaggcag cacaggagcg gtatggatgc gtttgagcgc ttgccccagg
     5281 gcgagccagt ggtggtcaga tagacgtact tcgtagccgt tatgcccgat tacaaagggg
     5341 gcaagaattg tcttataacc ctgcaagctg ccccagagac tgcccgagac tgtggggagc
     5401 ggggggatga tctgggtaat gccctggtca aagagatatt tgggcagagc aacggcaatc
     5461 tcatcaaaga ggccgcggcg taatttcagg aaataagact gcgccggtgt ggcaacccga
     5521 taaaccgccg tattttgatc agcgccaagg ggcaggaagt caatcgaagc ggcgggcagg
     5581 ttgtattcag tgcgcagaca ggcggcaatt aattcgtctg ggatggtggg cttttcaagc
     5641 atttggggta ttcttgttcg tcagagaatg gtttgattgt actccaaagc agagttgatg
     5701 ttgaactgac tgattgaacg gtaataacca agtgtataat tatgaagtcg gtattcttgt
     5761 tctggagggc tgccatgttc gagttccatg tttcccgtca ggcacgtgat cgctatcaat
     5821 ttgaccagac cttgttccgg tttgatggta atgtcatttt tgccaatttt catggggcgc
     5881 gcctttttgc tcaaaaaatg aatcagaagc gcgatctggc agcttacccg gagcaggcgg
     5941 ttaaggccgg gcagatcaat gcaatgggga tggtggatga gattctgcac ctggtggtga
     6001 gcctgtaccg gcggcagaaa aatcccaccg ccatctctgg agcgctggcc tggctggaag
     6061 aacgattggg cactgaacag gtcaaccgga tgctggtgcg gttcacagca gaatttccgc
     6121 cagttgtggt gtatcagcag caaatgacgg tggacgcata cctggcgggc agcacggatg
     6181 gcatcccaca ccgcgaggcg gcattggaag aactgctgat gctgtggatc tccaacaaaa
     6241 acccggcgtt ggcaccttac ctggaattat ttgatgactc ggggctgcaa tctgagacgg
     6301 tttatgcacg ggcgctgaaa gaactgcata tctattttga aacccagccc aaacacgggc
     6361 cggataatca gaacctgatt gatatgctgc gggcgccagc actggcaaac ccctattccc
     6421 tgaccgacca gttggaattc atccgcaccc gctggacaga gatgctgggg cggtatcttt
     6481 accggctgct gagcagcctg gacttgatca aggaagaaaa taagcagtcg ttcatggggc
     6541 cggggccaac ggtgatccca acttatcagc agcccggcag cggactggca gaatttgaag
     6601 gcgagccaga gcggttcagc ccagaccagg actggatgcc gcggctggtg ctgattgcca
     6661 agaacaccta cgtctggctg gaccagttga gcaagcagta tgggcgggaa atgcggcatt
     6721 tagatcagat ccctgatgaa gaactggacc gactggcgca gcaaggtttt accggattgt
     6781 ggctgattgg gctgtgggag cgcagccggg catcggcgca catcaagcag ttgtgcggta
     6841 acccggaagc aattgcctcg gcctattcgc tgttcgatta tcgcattgcc gatgacctgg
     6901 gcggtgagct ggcatacaac aacctgtcgg agcgagccgg gcagcggggc attcggctgg
     6961 cgagcgatat ggtgccgaat catatggcaa tcgattcgcc gtgggtagtg gagcatccgg
     7021 actggtttgt cagcctggat tacagcccgt tcccgggcta cacgttcaat gggcctgacc
     7081 tgtcggaaga tgggcgcgtg agcatcaata ttgaagatca ttattttgat cgaacagacg
     7141 cggcggtggt cttcaagcgg tacgaccaca ccaacgggca gactcgcttc atttaccatg
     7201 gtaatgacgg gaccagcatg ccctggaatg ataccgcgca gttgaattac cttaacccgg
     7261 cagtgcgaga agccgtgatt cagaccatcc tggcggtggc gcggcgcttc ccaatcatcc
     7321 ggtttgacgc agcgatgacg ctggcaaaga agcattatca acggttgtgg tacccggagc
     7381 ccggcagcgg cggggatatc ccctcacggg cagatcacgg catgacgcgg gatcaattca
     7441 acgcggcgat gccgattgag ttctggcgcg aggtggtgga ccgggtggca caggaagtgc
     7501 ccaatacgct gctgctggca gaagccttct ggctgatgga agggtatttt gtgcgcacac
     7561 tgggcatgca ccgggtgtat aacagcgcct tcatgaatat gctgcgcaat gaggacaacg
     7621 ccgggtaccg gcagctcatc aagaacacgc tggagtttga cccggagatt ttgaagcggt
     7681 acgtcaattt tatgaacaac ccggacgagc gcacggcggc ggatcagttt ggcaagggcg
     7741 ataagtattt tggcatctgc acggtgatgg cgaccctgcc gggactgccg atgtttgggc
     7801 atgggcaggt ggaaggcttc tctgaaaaat atgggatgga attccggcga gcctactggg
     7861 atgagcgcat tgatgatgga ctggtggaac ggcaccgccg tgagatcttc ccgctgctgc
     7921 accggcgctg gatgtttggt gaagtggcga acttcctgct gtatgatttc ttcacggggg
     7981 gcggggtgaa cgaggatgtg tacgccttct ccaatgggca gggggacgag cgggcactgg
     8041 tgattttcca taacaagtat gctcagacca gcggctggat tcgaatgtcg gcggcttatg
     8101 ttgtcaaggg gacgggggat caacgcaatt tggtgcaaaa aagcctggcg caggggctga
     8161 acctgcaccc tgccccgaac gcctatctgg tgtatcgaga cctgacgcgc ggcatggaat
     8221 acatccgccc cagtcaagaa gttgccgagc gcgggctgta cctggaactg aacgcttatg
     8281 aaacccacgt ctttgcggat ttccgggaag tacaggatga ctcctatcat tcttaccggc
     8341 agctctgcca gtatttgaac gggcgcggtg tgccaagcgt gacagaggcg ctgcgcgaac
     8401 tggtgctgat gccggtacag cagcccttcc gggagattgc caacccgggt tactttaact
     8461 atctgctgga tgcgcggctg gacctggaaa ccgaagcgct gccggcgggt ctgctggatg
     8521 aggcggagca aaaagccggc aacttgctgg atgggattca atacatgtct ggctatacgc
     8581 aaaaccgcgc tgaagtacag gcaggcatcc ggcgggcagt gcaatccacc ctatccctgc
     8641 ggcggctgga caccctgttc tcgctgtcca cggcggctgg tttccagaaa gcgctggatt
     8701 atctcgaaac cgggctggtg gacaaccggc gctggcgggt gctgttcggt tgggccttca
     8761 cccacaatct ggggcgcgtg gcgggcggca cagacccaga cgctcagacc caaagctggc
     8821 tggatgaatg gcagcttggc aagatgctga acgataacgc gctggcgatg ggttcggatg
     8881 aaggcaccgc ccggcggatg aatcaaacgc tgcggctgct gctcagccat cagaactggt
     8941 acagccagct tggcgccttg cccgttgaga agatcgtgcg cgaatggtta aatgcgccag
     9001 agatccagca gttcatcgga gtcaaccgct acaaggatgt gttgtggttc aatcacgagt
     9061 cctttgtgga actggtctgg tggatggcga cggcggctgt gctgaaggtg gccgccaacc
     9121 cgggcggcac agcggcagag ctgctggagc agactctggg cgcctatgag attgctcagg
     9181 cgctgctgaa agccgaggag aattcgggtt accaggtgga caagctgctg gcgctgctga
     9241 gtgaagcgga agaagcggca gaatagagtt tgagattaag caaagatagc ccctgcattg
     9301 tcggtaatgc aggggctatc ttttgaagaa gaatggctta tagataccag acaggaatga
     9361 tcttatcggt atccttaagg gtaatcaagc ggtcatacat gcagacaata cctccgctgc
     9421 cacgtttgag gccagacagg ttgtccaact taccaaacgc agagatatca tcgggccttg
     9481 gatcggcgtg ttttttgatt tcaatcggat gcagggtgtc tccggattgg atcaggaggt
     9541 caatctcagc gccatcacgg tcacggtaaa aatagagcgg cggttgcaaa atgcccgcat
     9601 tggtgtagct tttgtaaatc tcagaaatga caaagctttc aaaaaacgcc ccggcagagg
     9661 cgcccacaga caatacttcg ctgttaggcc aacgtcccag gtaggcagca aggccggtat
     9721 ccataaagta aagtttgggg gactttatgg ctcgcttaag gatattatta tgataaggct
     9781 gcagcaggta aatcaggctg gaggcttgca aaacggataa ccatctttca attgtcggct
     9841 gactgacgcc cacatcgcga gacagcgagg ccatattaag caactgcccg ttcatggcgg
     9901 caaccgcagt catgaactgg taaaacttca actcatcggc tacctgcgaa agttctcgga
     9961 catcgcgttc gatgtaagtg cgcacatagg tcgcataata catttgccaa tcggcctgac
    10021 tgtttgatac cagttctggc atagaaccac gaaagattgt ttgccagata cctttatagg
    10081 caaggggggg ggcgtcgttt tttcggcctt caaagtaggc ggcatcggga acaaaaggat
    10141 aattatttga aatgccaaag cgctccctca gtgaaatgcc cagaagattg aggatgccca
    10201 cccttccagc caacgattcg ctgacgtttt tcatcatgtg aaattgctga gagccagaca
    10261 agtaaaacaa tcccttctgc tttgtctgat cgatctgaat tttgatatat gggaaaaggc
    10321 caggcgcata ttggacttca tcaatgcaaa taggcggggg agaatccaaa aagaaggtcg
    10381 ctggttgatg aacagcctga ttgcgaagca cgggatcgtc caacgataca taggtgacat
    10441 cgcgaacgac ttcacgcagc agggttgttt tgcctacctg cctggcgccg gtcaccagca
    10501 cagcgccaaa catactggct gctttctgga gagcctgttc tatgtgccgc ttgatgtaca
    10561 taacgcaaat ccttttcggc tattataata tacaatatta taatagccga ttcggggcca
    10621 aaaatcaaga ggacaaagaa gattttttct aactgtgaac agcaaaccag ttgccgctga
    10681 ccggcggcag ggtgaggcgc agggcatcat cggttacggc aatttgctgc cggtcaatgg
    10741 ggctggtgaa gacctggcct tcggaggcat tcagcgccga agcggggatt tccaggtggg
    10801 ctttgtagcg agacgggttg gcaacggtga tcatctgatg cccatcggcg cggcgggcaa
    10861 aagcacaggc gccgtgactg ccctgggcaa gggggacaaa ttcgccgcgc cgcagggcaa
    10921 aagagccgcg gcgatgggcg atcaactggc gcagccaggt gcgcaggtcg tgattccatg
    10981 aagattcttc ccaggggaag gcccggcggc agtcggggtc tttgccgccc gccacaccaa
    11041 cctcatcgcc gtaatacaca gccggggcgc cggggaaaga gaataacagc aggtaagcca
    11101 ggcgcagctt gtccagattg ccatctaaaa cggtgagaac gcgctcggtg tcgtgggagc
    11161 caagcagcag gtacatggca aagacatttt tctgagaata gcgggacatc tgttcctgca
    11221 cctggccggt aaagccggcg ccgtccagct tgccggcgag atattcaaag attgaggtgc
    11281 ggagcgggta gtgcatcagg ccatcaaaat ggccggggct gacccagcga ggattgccat
    11341 cccagatttc acccagcaga taggcttcgg gattgacgga gcggacattg tagcgaaatt
    11401 cttcccagaa ggtgtcgtcg tcaatttcgt tgggcacgtc caggcgccag ccatcggcac
    11461 cctgttcaat ccagtagcgg gatacattca tgatgtactt gcgaacctgg ggattggccg
    11521 tattgaattt aggcaggctg gaataccccc accaggcttc gtagttcaga ttcttatggg
    11581 agccataggc tgaaaccggg aagtttttga catggaacca atcgcggtag ggcgactcgg
    11641 cttgattttc gagcagatcg ctgaaggcaa aaaagccgcg gccgcagtga ttaaacacgc
    11701 catccagaat gatgcgaatg ccggcgcgat gggcagcatc gaccagggcg tgaaagtcat
    11761 tcaaattgcc cagcttgggg tcgatttgga aataatcaaa ggcgttgtag cggtgattgg
    11821 aagccgccag gaaaatgggg ttgaaataga tggcattgat gccgaggtcc tgaagataat
    11881 ccagatggtg aaggaccccg cgaagatcac cacccataaa gccgtggcgc gttggcgggt
    11941 cgccccaggg ctggacgtta gcggggttga ttgaggcatc accattggca aaacggtcgg
    12001 ggaagatttg atagaaaacg gtatcttgaa cccaggcggg aatttttccg tgtgtgctca
    12061 ttgattctgc tcctgccgag ctttgcgctc agatggttcg agttttgcca gctcagaaat
    12121 tttgatatat gagtaaagaa taaagcccac aaacatgagg atggcgccaa aggccagaat
    12181 caaatcgtag agggtggggg tccacttgac ccagggcagg ttgaccagcg gattgcccgc
    12241 aaagaaaatc acggcattgg taatgataat cagcacgcgc atttcggtgg ggccgagttt
    12301 ggcgtatgaa atgcggaatt ttccggtgac gatggtatcg acatagacca ggacagacat
    12361 caacatgtag ccgatcaaag cgagggccgc aatcttgaag tcgatgtaag gagaaaggcc
    12421 caggcccaaa aagaccagca cttcatccag agcgtcaaca atatgatcga caaaaaagcc
    12481 gtatttggga cgctcaatat tgcggtagcg cgccagggtg ccatccagac tgtcgccaaa
    12541 ccagttcatg atcaggccaa ggctggccaa ccagaggaac caggggctgt gattggtcag
    12601 gatatagctg acaaagataa ccacggttcc aaagaagccg aatgcggtta acaggtctgg
    12661 gtgaacccag gcgggcatgt gggctgccaa ccagcgcaga gccgggcgtt ccaaaggacc
    12721 gagcagcatg ggattaaccc gggtgtgttg tgaacgatct ccaaaggaca tctgatctct
    12781 cctcaagacg ggatttggtc gagcgattcg ggccagaggg ggtaggtcat ggcccactgc
    12841 tcaggatgcg cctgcaggac ttcttcagca aggcggagaa ttttttcggc gttggcaata
    12901 atttcagtat cccgatcggg ataagattcc agccagatgg gctcggaaca atcgagcaaa
    12961 tatttgccct tgggctgcga taaacaatat acgaaataga ccggggcttt gttgcgtaga
    13021 gccagaggaa tgtaagctac gggaagcgcc gtaggacgtc caaagaagcg cggatggtgg
    13081 gttgattcag gccaggggcg atccagacct gtcagaacgg tgccgccgct ctgcaggcgc
    13141 tggcgggcag cctggagcgc ttcgatattc aggggcatta tttcaatgcc gctgagacga
    13201 cgaagttcgt tttgaagctg atagccgccg ggcggctggg gaaaagacag gacaagaaat
    13261 ttcaggccag cctgaaccag cgccatcccg gccaggtcaa atgagccggt gtggcttgcc
    13321 acaaagacag ctccatgctg accctgctgg cgttctgcaa tcacatcgag catatggggg
    13381 gtgaattgga ccattcgacg ggcgcggcgg ggggcgcgga ggttatggta atggtcaaaa
    13441 agagcgctgc ccgaggcttg aatgacctga caggcacgcg catccaattc cggggcggtc
    13501 aatttttggc cgctgagcac ccattgattg gcgcgaatcg cctgaaccat ggggcgctga
    13561 cggcgacaga caatgaggcg cgccacaaga cccaccaact gctggccaac ccagcgcggc
    13621 atcaactggc tgagggtcag catgaggctg accgcaaagc ggctgttgga gaaacgctga
    13681 agattgaggc gtgggcagcg ggggcgttcg gcggtcatct tgaaatttcg gctaatagtt
    13741 tttcgggttg gttggcatag gtctcgcgcg gcaggtcgcc ggcagcgagt gcggatagga
    13801 tgcgcgtcag ggattgattt accggggcaa aacgtttgaa ggcggcagcc tggcgggcca
    13861 ccgctccgtt gagatattcc acctcgctgc gggtacggcc accgtataaa tcaatgtgga
    13921 aagaaggcat cttgtcgccg cgtcctttgg tgaggccgcg tttgagtaag ggctggctga
    13981 ccaggggagg cagataccca aaagcccagg ccagggcgcg aaccggcgtg ccgggcaaat
    14041 cacagaccgg caaaggccag gcgcgcatga ccgataaagc ctcgcggaac tgggttactt
    14101 ccaggcggta gatgcctggg tgcgagaata cctgggcggg agtccagttg aggatggcag
    14161 aagtggcgtt ggcgggcaga ttggtgagaa gcttggacca cttcatggcg ttggggtggt
    14221 tgtagatctg ggcattcagg ccagcctggt tgaagacagt ggtcaaatcc agaacatggg
    14281 gatggacata agccaggccc accccgcgca ggcgttcaag cacaatttga cccaccccca
    14341 gccgccccac agcactggta acagtgccgg ggatcacccg agaaaagcct accagactgc
    14401 gcagatgatt ttcgttttca acaccatttt ggaggcagat caggggaggc aactgggcct
    14461 ggtgaggccg gagcatgtct ataagcgccg gggtatcata ggatttgatg gcgacaatgg
    14521 cataatcaaa tgggccgcgg gacagggcct cctcgatgga atctacaatg attggggcgt
    14581 ctatctgccg ctcgcccccc ggcaggacca gccgcaaacc gctttgccgg gctgcagcgg
    14641 cgccttcagg gcgctccaaa aagacgacct cttgcccgtt gagggcgagg ctgccgccaa
    14701 tgtacgtacc aattgcacca acaccgaaac aaagaaaacg catccgggcg tccttttctg
    14761 agggtgaaag atgggtgcta ctctgttacc agccgcgggc tccccgcaga aagacatcgc
    14821 cgggcatggg gcgcttgccc gcgggctgta attccagcaa aagcaggcag ccgagggcag
    14881 tgccaattgc cggaaagcca gccactatac cgcgctgacc gggagacagg tgatcggcag
    14941 gatgggcaga ggcacgctga atttttaaca ccgcatcggc ccaaaaggtg taggcgcctg
    15001 gccagggctg ataggcacgg acctggcgtt ccagttcaac ggcggggcga gaaaaatcga
    15061 gcaggccatt ttctttctgg atcatcggag catagaccgc gagggcatca tcctgcggct
    15121 ggggaataat tttaccgttc agataagcgg gaagggtttc gattaacaaa tcggcgccaa
    15181 gctgagccag cctgtcactg agtgcaccgg cagtatcttc aggcagaatg gggatggcgc
    15241 gctgggccag cacggggcct gtatccacac cggcatccat gcgcatgatg gaaacgccgg
    15301 tctgggcatc gccagtgcgg atggcagcct gaatgggcga ggcaccgcgc cagcggggta
    15361 agagcgaggc gtgaacgttg acgcagccat gcgggggcag gtccaggacg ttttggcgca
    15421 gaatttgacc gaaggcggcg acaacaatga gatcgggcgc ccaggcagcc agttgggcct
    15481 gcgcctcggg ttctttaagg cggttgggtt gaatgaccgg cagggagagc gactgggcca
    15541 gaattttcac cggcggagaa gccagggttt gcccccgccc agcgggacgg tcgggctggg
    15601 tgatgacgcc gatgaccggg taggcctcag tcaaacggcg caggacaggt acggcgaagt
    15661 cgggcgagcc catgaaaacg atgcggacat ccatggcata attttagcac atcttggagg
    15721 tttgacgatg gaatggcaaa ggtctggaca ttgtctgaca aaactcaatt ggcgctcaat
    15781 tttacctgtg caatttataa aattaagcta taataggttc attaatccct ttctaacatt
    15841 tggaggaatc aatgaccgac caaaccccta tcacccctga ggtggccttg ccccgaaaaa
    15901 gtggacacga ttaatgtcct gaaggttgct cgaattc
//
LOCUS       DAOPVS010000036        15710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3818_length_15710_cov_52.202619, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000036 DAOPVS010000000
VERSION     DAOPVS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15710
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3818_length_15710_cov_52.202619"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1355
                     /locus_tag="PKL11_04850"
     CDS             174..1355
                     /locus_tag="PKL11_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ12964.1"
                     /translation="MLSKQYTLSRSWTQPRAHYTHIDIELTERCNNACIHCLINQPPN
                     VSHLISREMDTIWVKEILTQAADLGCLTVRFTGGEPLLRPDFPELYVFARRLGLYVIL
                     FTNARLITPETAALLTRYPPGRPVEVSVYGMHAESYDAVAASPGAFDEFWQGINCLRQ
                     FNIPLIVKQSLLPSNRREQPEFEAFAAGLPGMIHRPGYTMNFELRSRRDNPAKNRLIQ
                     SLRLTPEETVQQLSHNPGYVKNMQAFAAKFMRPPGDKLFTCGAGLGLAVDAYGNALMC
                     MGLHHPDLSYPLNAQNHRALRMAVSEQFPIWREMQATNPDYLQRCARCFLKGLCEQCP
                     SKSWQEHGTLDTPVEYYCQVAHAQARYLNLLTEDENAWELEPAVWRQRLKSFTTSCSE
                     S"
     gene            1370..1678
                     /locus_tag="PKL11_04855"
     CDS             1370..1678
                     /locus_tag="PKL11_04855"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNZ12965.1"
                     /translation="MELNLTGESICCPSEDIVAREIAGELIIVPLASGIGDMEDELYT
                     FNETGRAIWDRLDGVCSLHQIAVNLSEAYFAPLDEIESDVLGLMTELARRRIVVVKPV
                     "
     gene            1699..2169
                     /locus_tag="PKL11_04860"
     CDS             1699..2169
                     /locus_tag="PKL11_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNZ12966.1"
                     /translation="MDGPLLQIPNADFAALMSAVVARGLPFRFQASGSSMAPFIHNHD
                     VVIIEPLPDRLRVGDVIAYTCPASSRLIVHRIVRLSQSGYTTRGDNTRQLDPPITRSH
                     ILGRVTHVERNNKPHSWGLGPHKTLIAALSRRGWLIPLINVLRPHFRLFKRISQ"
     gene            2166..3281
                     /locus_tag="PKL11_04865"
     CDS             2166..3281
                     /locus_tag="PKL11_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ12967.1"
                     /translation="MSQTSIIEIQDFSLWDKTRHSRSLHSFDLELTARCNNNCRHCYI
                     NLPASDPQARAEELSPSEISTIADQAVELGALWCLLTGGEPLLRPDFAEIYMLLKRKG
                     LLISVFTNATLIRPEHITLFRQYPPRDIEITVYGASQTTYERVSRVPGSFAAFTRGLE
                     ALIAAGIRVRLKAMALRSNLDELGAIAAFCRTRTKDYYRFDPQLHLRYDRDPYRNVEI
                     ESERLTPAEIVVLESTDPERMGALRASCDSLINPDFGHQNSNRLFRCGAGSGSFTVGF
                     DGTFRLCASLCAPDATINLRTTSLRTAWENFAPRVRSMHSTNPEFLSTCNQCAIVNLC
                     LHCPAHAYLETGAMDGATPYFCQVAHARQAALESDAQ"
     gene            3373..4515
                     /locus_tag="PKL11_04870"
     CDS             3373..4515
                     /locus_tag="PKL11_04870"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNZ12968.1"
                     /translation="MNAVSVEQWQTILKLAGHYGVAPILHHRLEYYDVALPDEIAWAL
                     KQAYLWNTAHNLRNDHELTRILRLMQAHNIPVILLKGVYLASFIYADLGLRTMTDIDL
                     LIPAADIEKSIDALQSLNFVATRPFWPAVDLALHHAPPLTKENLIVELHWRLTHETNL
                     PGIDHAGIWARSEPIQINHTQAAALCLSDLIFHLIVHASYHHQFHNQLRTLVDLAEVL
                     QKHQPDLDWSEIVQLIRTCRAERGAYITLRLAADLLGAQIPSGVFQELCPSGWADTVL
                     GWAQFQLFQLDPVLSENYIRVFHGAGFADKLAALRRGLFPSRTVMAAIYALPPESKKL
                     VWLYPRHIISRLSKYFKPAWRLLLGDRRQSLESKSDQSLRDWLHTA"
     gene            complement(4522..6165)
                     /locus_tag="PKL11_04875"
     CDS             complement(4522..6165)
                     /locus_tag="PKL11_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HNZ12969.1"
                     /translation="MSGIIRFLSENPLLLLFIVAGIGYPLGRIKIGGASLGVSAVLFV
                     GLAIGALSPDLKLPASIYELGVVLFVYCIGLSSGSIFFSSMKRQGLKANLLILGGLLF
                     AAGLAALAHYLFDVKATMVSGMYAGSLTNTPALAGVVDLIKTTAPADTVEMLMAEPVV
                     AYSLSYPMGTLGVILVIFILQRAWKIDYAAEARQARELGPLNEPLQSITLRVLNTQTL
                     TIAELIRQNDWNVVFGRYKRAEAVKLVTGPLLLEAGDYISLVGTIEELNSVQKALGEA
                     VEERLEFDLSHFDKRRVFISNPKITGRRLSELNLVNTYGAMVTRLRRGDLEVLPHGQT
                     TLMLGDQVRVIAPHNQMENVIQLFGDSYRGVSEIDVLTFSLGLALGLLLGMVPIHLPG
                     GLTLKLGMAGGPLIVALVLGALGRTGPVIWQIPYSANLTLRQIGLILFLAGIGTRAGY
                     SFISMITQGNGPIIFIAGALVTCLTMLVTMWVGYKLLHIPMGLLTGMVAGLQTAPAAL
                     GFATEQSHNELPNIGYATVYPVAMISKILLAQALLLMFM"
     gene            complement(6169..6918)
                     /locus_tag="PKL11_04880"
     CDS             complement(6169..6918)
                     /locus_tag="PKL11_04880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ12970.1"
                     /translation="MNTAPDSSQNQAPVRPPVFWHLPARTPIVTYVLIGLTVTAFLGQ
                     FISEAALGGDLLAAFGMKINSAIMQGEIWRLLTPMFLHGGLAHIAFNMYALFSLGVGL
                     EKQYGHGRFLMLYLLGALGGNTLSFLLTPSNSLGASTAIFGLIAAEGVFIWKNRRMYR
                     NPQKELVNVALIVGVNLFLGLGGGIDNFGHLGGLIAGIMFAWFAGPQWTGTVQPTGLH
                     IIDQQRPGRMWLVGGLVALFWCVLAIVKMVG"
     gene            complement(6915..7541)
                     /locus_tag="PKL11_04885"
     CDS             complement(6915..7541)
                     /locus_tag="PKL11_04885"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNZ12971.1"
                     /translation="MCDNSLSFDLLHPHPLLIVISGPSGVGKDSVLQALKARNLPMHF
                     VVTATTRSPRPEEKHGVDYFFVSLEEFERMIAEDELIEHAIVYNQYKGIPKAQIREAF
                     ASGKDVVLRVDVQGAARLQTLYPNALMIFLLPSNEQEWIDRLRRRNTETPENLVLRVE
                     TARKEIESLPRFDYMVVNAEDRLEQAADTIVAIIQAEHHKVCHRIINV"
     gene            7625..8008
                     /locus_tag="PKL11_04890"
     CDS             7625..8008
                     /locus_tag="PKL11_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ12972.1"
                     /translation="MPTYEYRCLDCRKRFIIFLTYAEYGVKPVECRHCGSQKVERRIG
                     RIRVARSDESHLESLADPSQLEGLDEDPRALGRMMRQMKGTVGEDMGPMFDEVVDRLE
                     AGQSPEDIDSAMPDLGDSLGAGDDF"
     gene            complement(8005..9285)
                     /locus_tag="PKL11_04895"
     CDS             complement(8005..9285)
                     /locus_tag="PKL11_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12973.1"
                     /translation="MKQARGFNRLLVIGFLFLWLVQGSTLLLTNADEHLRRFTRAYEF
                     DFSVWMLDALRIKFAQAALDAPSYLSEAEQAALVKDYLAKIAKLNKLKADIEWLYTSP
                     TVADPTIASKPLVRDQLQLQAELKQLGPLAESIIQAQLNAILLEEGLTPVPPPLYHVT
                     DLPYALIISPRDVIRQDYDISLLPDMTMPEITELEQRIEKELNVSALIEPVGGIGVYP
                     TMVMATSSITWLPEVVAHEWTHNYLTLHPLGMGYFASGEMRTINETTASIVGVELSRA
                     LLAQYFPQDLPPEPLPTWPLPAPFEEIYPGLDRSQPPAFNFRVEMHTTRVEVDRLLAE
                     GKIEEAEQYMESRRLFLAEHGYNIRRLNQAYFAFHGAYAGGTADAAYGAQGRDPVGPA
                     VRALRENSGSLADFLKQIAWISSFEELEEAVAEP"
     gene            9426..>9633
                     /locus_tag="PKL11_04900"
     CDS             9426..>9633
                     /locus_tag="PKL11_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12974.1"
                     /translation="MKRKTIVTIGIILALVVAGYFLVRYLQQQKASAGSEYQTAVVER
                     GSLTAIVGATGTVHANQSATLAWET"
     assembly_gap    9634..9733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9734..10981
                     /locus_tag="PKL11_04905"
     CDS             <9734..10981
                     /locus_tag="PKL11_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ12975.1"
                     /translation="GATGTVHANQSATLAWETSGRVSTIDVAVDDQVTADQVLATLAQ
                     DSLSQSIILAEADLIAARRSLDNLLNSNTAAAQAQLALVNAQESFDDASQSRANYDYN
                     LYTADTIEIARTTYYLALDKINKYEEIYENFKDKPEDDPLRAQAANNLANARQNADQA
                     YDNLNYLLGGPDPYDISVADANIALAEARLTDAQREWDRLKNGPDPDDVRSAEVRIAA
                     LEAALDSKNLRAPFAATVTEVKSLPGDMVNPGTVSFRIDDLSHLLVDVDIPEVDINRI
                     QIGQAVNLSFDAITDRQYTGEVIEVARVGSTAQGVVNFKVTIELTDADEAVRPGMTAA
                     VNIIVEQLDDVLLVPNRAVRSSGGQRVVYVLRLGIPTPVPVEIGATSDLVSELLPGEV
                     KEGDVLVLNPPTQIIPADGMFGR"
     gene            10987..11679
                     /locus_tag="PKL11_04910"
     CDS             10987..11679
                     /locus_tag="PKL11_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ12976.1"
                     /translation="MTTEDTVIVATGLTKVYKMGEVEVHALRGANLRIRRGEVVAIMG
                     PSGSGKSTLMNILGCLDIPTSGEYCLDGECISQLRDDQLAIIRNRKVGFVFQSFNLLS
                     RTTALSNVELPLRYAGIPARDRLDRASAALEAVGLADRMRHKPTELSGGQQQRVAIAR
                     ALVNDPAILMADEPTGNLDSKSGKEIMELLLNLNRERHTTLIIVTHDQLVGQQAQRII
                     RISDGVVVEGGE"
     gene            11676..12902
                     /locus_tag="PKL11_04915"
     CDS             11676..12902
                     /locus_tag="PKL11_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ12977.1"
                     /translation="MNLGQAIIEALESISGNKLRSGLTILGIIIGVGAVIAMLGVGQG
                     AQNTITGSISGIGSNLLFVFSGNMEQEVRNEKPLTLSDAEAMADPFQAPSVAAVAPVI
                     QGSGEITFGAETASTSLYGVTPDYTVVRNYGLIEGEYIDEQHLLGQSSVVLLGPDVAE
                     KLFGRTDGITGETVRINGLPFRVIGILESKGGGSFGSQDDVALVPFSTAQSRLIRRSR
                     DRVDVVFVQVASAEQVSDAREEIAQILRTRHRTALGEDDFTIFSQEDFVATAQTITGV
                     FTIFLGGIAGISLLVGGIGIMNIMLVSVTERTREIGLRKALGARRRDILIQFLTESSL
                     LSLIGGVVGIGLGWLIAFIVGKIAAASGTPFYPSIGVDSILLATLFSTAVGLFFGLYP
                     ANRAAGLEPVEALRYE"
     gene            13098..14039
                     /locus_tag="PKL11_04920"
     CDS             13098..14039
                     /locus_tag="PKL11_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNZ12978.1"
                     /translation="MSGWKVLLTDGLEEKGKAILAQSAEVVDRTGISADELLQIVGEY
                     DAVIVRGRTKVIPAVFDAASRLKVVGRAGVGVDNIDLNAARAHNVIVVNSPLATTIAV
                     AEVTMALMLSVVREVARADATMKAGKWIKKELEGAELHAKTLGVIGFGRIGAAVGQRA
                     AAFGMKIIGYDPLIPADEIQRRGGKPVTMDELLAEADIITMHIPLTADSKNMLNADAF
                     AKMKQGVYLVCAARGGVIDEEALLASLESGKVAGAGLDVFATEPPGLTALVSHPRVVC
                     TPHIGAQTVEAQERAAVDISSEVLAALRGDALRWRVA"
     gene            14055..15554
                     /locus_tag="PKL11_04925"
     CDS             14055..15554
                     /locus_tag="PKL11_04925"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNZ12979.1"
                     /translation="MTKIEQLEALTRELGAIQRASAVLGWDAQVNMPAGGAEERGFQL
                     GVLAELSHKRFVNDETHNLIVAAEAEAASLDPDSIQARLVKYCRRDYDKATRVPAAKV
                     AEFAQVTAVSQEAWARARSENNFAAFLPHLERVIELRREYASYFAPYDHVYDPLLDDF
                     ERGMKTAEVQAIFNQLRPVQVELVHAIAARPEIENSFLYQNYPEQAQWDFGVEVATRM
                     GYDWNRGRQDKSAHPFTTSFGLGDVRITTRFDTGYVGSALFSTMHEGGHALYEQGFDP
                     ALRGLPLADGASMAVHESQSRLWENLVGRSLAFWKFFYPRLKEYFPTQLANVDLQTFY
                     RGINKVKPSYIRVESDEATYNLHIMLRLELEIALMEGSLKPADLPAAWNARFQEYLGL
                     TPPTDKVGVLQDVHWSHGLIGYFPTYALGNLVSCQLWEVIQQAIPDLESQIEHGELGT
                     LLAWLRQNVHTHGSMFEPQELVQKITGSRIDPQPYMRYLKTKFGQIYGL"
BASE COUNT         3441 a         4681 c         3931 g         3557 t
ORIGIN      
        1 aattttggcc cgcagccagc ctgaagaagc tgttttatta acctgcaaaa ccgatagcgt
       61 tttagttgga cccagtactt cttcaaattg ttcgaaatat actggagccg gctgtgacaa
      121 taattggccc agctaggtta ttctagagcc atttttcctg cctggttcgc aaaatgctca
      181 gtaaacaata caccttaagc cgaagctgga ctcaacctcg cgcccattat acgcacattg
      241 atatcgaact gactgaacgc tgtaacaacg cctgcattca ctgcctgata aaccagcccc
      301 caaacgtctc ccacctcatc tcgcgcgaga tggataccat ctgggtcaaa gaaatcttga
      361 cgcaggctgc cgacctgggc tgcctcacgg tgcgctttac tggcggcgaa cccctgcttc
      421 gccccgattt tccagagctt tatgtttttg cgcgccgcct gggcctctat gtcattctat
      481 tcaccaatgc ccgcttgatc acccccgaaa cggcagcact tttgacccgc tatccgcctg
      541 ggcgtccggt tgaggtttct gtttatggga tgcatgccga atcctatgat gctgttgccg
      601 cgtctccggg cgcttttgat gagttctggc agggcataaa ctgcttacgt cagtttaaca
      661 ttcctctaat tgtcaaacag agccttttgc cttccaaccg ccgtgagcag ccagaatttg
      721 aggcctttgc tgccggttta cccggaatga tacatcgccc tggttacacc atgaattttg
      781 aattgcgatc ccgccgtgat aacccggcca agaaccgttt gatccaatct ttgcgtctca
      841 caccagaaga aacagtccag caactgtctc ataatcccgg ctacgttaaa aacatgcaag
      901 catttgcggc aaaattcatg cgcccgcctg gcgacaagtt atttacctgc ggcgccgggc
      961 ttggccttgc tgtagatgcc tacggaaacg ccttgatgtg catggggttg catcaccccg
     1021 atttatcgta tcctctgaat gcccaaaatc atcgtgcgct tagaatggct gtatcagagc
     1081 aatttccgat ctggcgtgag atgcaggcca ctaacccgga ctacctgcag cgctgcgccc
     1141 gttgttttct caaggggttg tgtgagcagt gcccatccaa atcatggcaa gaacacggca
     1201 ctttggatac cccggttgag tattactgtc aggtagccca tgcccaggca cgatacttga
     1261 acctgttgac cgaagatgaa aacgcctggg agcttgaacc agcagtctgg cgtcaaaggt
     1321 tgaaatcgtt tacaacatct tgttctgaat cgtaaaagga ttagcgctta tggaattaaa
     1381 cctgactggt gaaagtattt gctgcccatc tgaagatatt gtcgcccgcg agatcgctgg
     1441 cgaactgatt atcgtccctc tggcatccgg tattggagat atggaggatg agctttacac
     1501 cttcaatgaa accgggcgcg caatctggga tcgcttggat ggcgtttgtt ccttgcatca
     1561 gatcgcggtt aacctgtcag aggcatattt tgctcctctg gatgagattg aaagtgatgt
     1621 gctgggctta atgactgagc tggcgcgtcg ccggatcgtg gtcgttaaac cggtgtgagc
     1681 aggcggcaaa tgcaaaccat ggacggaccg cttcttcaaa tccccaatgc agactttgcc
     1741 gcattaatgt ctgctgttgt tgcgcgcggc ctgcccttcc ggtttcaagc ctcaggctcc
     1801 agcatggcgc ccttcatcca caatcacgat gtcgtcatca tcgaaccgct ccctgatagg
     1861 ctgcgcgttg gcgacgttat cgcctacacg tgcccggcat cttcccgttt aattgtgcat
     1921 cgcattgtcc gcctatctca atccggctac acaacccgcg gggataatac ccgccaattg
     1981 gatcccccga ttacccgatc ccatattctg gggcgagtca cccatgtgga acgaaataac
     2041 aaaccccact cctggggttt gggaccgcac aaaaccctga tcgctgctct gtcccggcgt
     2101 ggttggctga ttccgctaat caacgttttg cgtcctcact ttcgtttatt caagcgaatt
     2161 tcccaatgag tcagacttca attattgaaa ttcaagattt ttcgctgtgg gataaaacca
     2221 ggcattcccg aagcctgcac tcatttgacc ttgaattaac agctcgctgc aataataact
     2281 gccgtcattg ttatatcaat ctacccgcca gcgatcctca ggcacgcgca gaagaattat
     2341 cgccctctga aattagcaca atcgccgatc aggcggttga actaggcgcg ctttggtgtc
     2401 tgctcactgg cggcgaaccc cttttgcgcc ccgactttgc cgaaatttac atgctgctaa
     2461 aacgcaaagg tctgttgatc tccgttttta cgaatgcaac cttgatccgc cctgaacaca
     2521 ttacactttt tcgccaatat cccccgcgcg atattgaaat cacagtctat ggcgcttcac
     2581 aaacgactta tgagcgcgtg tcgcgggtgc ctggcagctt tgcggctttt acgcgtgggt
     2641 tggaagcgct gatagctgca ggtattcgtg tccgcctgaa agctatggcg ctgcgctcca
     2701 acctcgacga gctaggcgca atcgctgcct tttgccgcac acgtaccaaa gactattatc
     2761 gctttgatcc ccagcttcac ctgcgctacg accgggatcc ttaccgcaat gtcgaaattg
     2821 aatctgagcg tttgaccccg gcagaaatcg tcgtgcttga aagcactgat cctgaacgca
     2881 tgggcgccct gcgcgccagt tgcgattctt tgattaatcc cgactttggt caccaaaaca
     2941 gcaatcgtct gtttcgctgc ggtgcaggca gtggcagctt tacggtgggt tttgatggca
     3001 cctttcggct ctgcgcatca ttatgcgcgc ccgacgcgac cataaatctt cgcaccacca
     3061 gcctccgaac ggcctgggaa aactttgctc cccgggtacg cagcatgcat tctaccaacc
     3121 ccgaatttct cagtacctgt aaccagtgtg ctattgtcaa tttatgtttg cactgccccg
     3181 cccacgctta cctggagacc ggcgcaatgg atggcgctac cccctacttt tgtcaggtcg
     3241 ctcatgctcg tcaggcagcc ttggaatcag atgcgcaata acccgaagac gcttatttcc
     3301 gccctgcccc ctgaagtatc gctcttactc ctctgtttgc gtagcaaaga tcagtctgaa
     3361 ctccgatctc aattgaacgc agtttctgtc gagcagtggc aaaccatctt gaagctggcg
     3421 gggcattatg gcgttgcacc tatattgcat catcgccttg aatattatga cgttgctctg
     3481 ccagacgaaa tcgcctgggc gcttaagcag gcctatcttt ggaacacggc tcacaatctg
     3541 cgcaatgacc atgaacttac gcggatattg cgtctcatgc aggcgcacaa tattccggtg
     3601 attctgctca aaggcgtcta ccttgccagc tttatttatg ccgacctcgg gcttcgcaca
     3661 atgactgata tagatctcct gatccctgcc gccgatatcg aaaaatccat tgatgctttg
     3721 caatcactga actttgtcgc cacgcggcca ttttggcccg ctgtggatct ggcgctgcac
     3781 cacgctcccc cgttgacgaa agaaaacctg atcgttgaac tgcattggag gctgacccat
     3841 gaaacaaatt tacccggcat cgatcacgct ggaatctggg cacggtcaga accaatccaa
     3901 atcaatcaca ctcaggcggc tgccctttgt ctgagtgacc tgatttttca cctgattgtg
     3961 cacgcctcat atcatcatca attccacaat cagttacgca ctctcgttga tctggctgaa
     4021 gttttgcaaa aacaccaacc cgatttggat tggtcagaga ttgttcaact aattcgcacc
     4081 tgccgggcag aacgtggtgc ttacatcacg cttcggctcg cagccgacct gttaggcgct
     4141 cagattcctt ctggcgtctt tcaggaactc tgcccttcag gttgggcaga cacggttctg
     4201 ggttgggctc agtttcagct gttccagttg gatcccgtgc tgagcgagaa ctatattcgc
     4261 gtctttcacg gggccggctt tgcagacaag ctggccgcct tgcgccgggg tcttttccca
     4321 tcaaggactg taatggctgc aatctatgct ctcccccccg aatcaaaaaa acttgtctgg
     4381 ctttacccgc gtcacatcat ctcgcgcctt tcgaagtact ttaagcctgc ctggcgcctg
     4441 ttactgggag accgtcggca atccctggaa tcaaaaagtg accagtctct ccgagactgg
     4501 ctgcacacag cctgaatcct ttcacataaa catcaacagc agcgcctgcg ccagcaaaat
     4561 cttgctgatc atcgctaccg gatacactgt tgcataacca atattgggca gctcattgtg
     4621 agactgttca gtcgcaaagc ccagcgccgc tggtgccgtt tgtagtcccg ccaccatccc
     4681 cgtcagcaac cccatgggga tgtgcagcag cttataaccc acccacatgg taaccagcat
     4741 cgtcaggcag gtcaccagcg ccccggcgat aaagataatc ggcccgttcc cctgcgtaat
     4801 catgctgata aacgaatacc ctgcccgtgt gccgatgccc gccagaaata aaatcaaccc
     4861 gatctgccgc agtgtcaggt tggcgctgta aggaatctgc cagatcaccg gtccggtgcg
     4921 gcccaatgcg cccagcacca gcgccacaat cagcggtcct ccggccatgc ccagcttaag
     4981 cgtcagcccg ccgggcaggt ggatgggcac catgcccagc agcagcccca gcgccagccc
     5041 caggctgaaa gtcagcacat caatctcgct cacgccccga taggagtccc caaagagctg
     5101 gattacgttt tccatctggt tatggggcgc aatcacccgc acctggtcgc ccagcatcag
     5161 tgtggtctgt ccgtgcggca gcacttccag gtcgccgcgc cgcagccgtg tgaccatggc
     5221 gccgtatgtg ttcaccagat tcaattcgct caggcgccgc ccggtaattt tgggattgga
     5281 aataaacacc cgtcgcttgt caaaatggct caggtcaaat tccaaccgtt cttcaaccgc
     5341 ctcgcccaat gccttttgaa ctgagttcag ttcttcaatg gtccccacca gtgagatata
     5401 atcccctgct tccagcagca gcggtccggt gacaagtttg accgcctccg cccgcttata
     5461 tcgcccaaac accacgttcc aatcgttttg ccggatgagt tcagcaatcg tcaacgtctg
     5521 agtgttcaat acccgcagag tgatgctctg cagcggttca ttcaacgggc ccagttcacg
     5581 cgcttgccgg gcttctgcgg cataatcaat cttccaggca cgctgcagta taaaaatcac
     5641 cagaatcacg cccagcgtcc ccatggggta ggacagtgaa taagccacca ccggttcagc
     5701 catcaacatt tcaactgtgt ctgctggtgc agtcgttttg atcaggtcaa ccaccccggc
     5761 taacgcgggc gtattggtca ggctgccggc atacatcccg gaaaccatcg tcgccttaac
     5821 atcaaataga tagtgcgcca gcgccgccag tccggcagca aagagcagcc cacccagtat
     5881 cagcaaattg gccttcaatc cctgccgctt catcgagctg aagaaaattg agccgctgct
     5941 caatccaatg cagtacacaa acaacaccac gcccagttca taaatggagg caggcagttt
     6001 aaggtccggg cttaacgccc caattgccaa ccccacaaat aaaaccgccg aaacccccag
     6061 gctcgccccg ccaattttga tccgccccag cggatacccg atgcctgcca caataaatag
     6121 cagcagcaac ggattttcgc ttagaaagcg aatgattcca ctcatgcctt acccaaccat
     6181 cttaacaatg gccagaacgc accagaataa agccaccagt ccgccaacca gccacatccg
     6241 ccctggacgc tgttggtcaa taatatgcag cccggtgggc tgtaccgtcc ctgtccattg
     6301 gggcccggcg aaccaggcga acatgatgcc cgcaatcaac ccgcccaggt gcccaaagtt
     6361 atcaatcccg ccgcccagcc ccaggaacag gttgacgccc acaatcaatg caacattcac
     6421 cagctctttt tgcgggttcc ggtacatccg ccgattcttc cagataaata caccttccgc
     6481 agcaatcaac ccaaaaattg cagttgaggc acccagagaa ttgctgggcg tcagcaaaaa
     6541 ggacaatgta ttgcccccca gcgcccccag cagataaagc atcaggaagc gcccatgtcc
     6601 atattgtttt tccaacccga ccccaagtga aaacaaggca tacatattaa aagcaatatg
     6661 cgccagtcca ccgtgcagga acatgggcgt caataatcgc cagatttctc cctgcatgat
     6721 cgctgaattg atcttcatcc cgaacgccgc caggagatcg ccccccaaag ctgcctcact
     6781 gatgaactgg cccagaaagg ccgtcaccgt caggccaatc agaacatagg tcacaatcgg
     6841 cgttcgcgcc ggcaaatgcc agaacaccgg cggtcggact ggcgcttgat tctgcgagga
     6901 atccggcgcc gtgttcatac gttgataatc cggtgacaca ccttgtggtg ttccgcctgg
     6961 atgatcgcca caatcgtatc cgcggcctgc tccagccggt cttccgcatt gaccaccatg
     7021 taatcaaacc gcggcaggct ctcaatttcc ttgcgcgctg tttcaacccg cagcaccagg
     7081 ttctcgggcg tttccgtgtt gcggcggcgc agccggtcaa tccattcctg ctcattggag
     7141 ggcagtaaaa agatcatcag cgcgttgggg taaagggttt gcagccgggc cgctccctgc
     7201 acatccaccc gcagcaccac atctttgccg ctggcaaacg cctctcgaat ttgtgccttg
     7261 gggatgcctt tgtattgatt gtacacaatc gcatgttcaa tcagttcatc ttccgcaatc
     7321 atgcgctcaa actcttccag cgacacgaag aaataatcga cgccgtgctt ttcttcgggc
     7381 ctgggggagc gggtggttgc tgtaaccaca aagtgcatcg gcagattgcg cgctttaagc
     7441 gcctgcagca ccgaatcctt gccaacgccg gagggcccgg agatgacaat cagcagggga
     7501 tgaggatgca gcaaatcaaa tgacagggag ttatcgcaca tggcagaata cccaaattaa
     7561 cagaattctt acgtacattg attataatag tatttgcccc gctggggtgt ggaggaatgt
     7621 cactatgccg acctatgaat atcgctgcct ggattgccga aagcgtttta tcatcttttt
     7681 gacctatgcc gagtatggag tcaagcccgt tgaatgccga cattgtggca gtcagaaggt
     7741 ggaacggcgc attggccgca ttcgcgtggc ccgctcagat gagagccacc tggaaagtct
     7801 ggcggatccc agccaacttg aaggcctgga tgaagacccc cgtgccctgg gccgcatgat
     7861 gcgccagatg aaaggcacgg ttggcgaaga tatgggcccc atgtttgatg aagtggtgga
     7921 tcgcctcgaa gccggtcaat cgcctgaaga tattgacagc gccatgcccg atctgggcga
     7981 cagcctcggc gccggcgatg acttctaagg ttctgccacc gcctcttcca gttcttcaaa
     8041 cgaactgatc cacgcgattt gcttcaaaaa atctgccagc gagcccgaat tttcccgcag
     8101 cgctctcact gctggaccca ccgggtcgcg tccttgcgca ccataggccg cgtctgctgt
     8161 gccaccggca taagccccat gaaaagcgaa gtatgcctgg ttcaaccgcc ggatgttgta
     8221 cccgtgttca gccaggaaca accgccgaga ttccatgtac tgctcggcct cttcaatctt
     8281 gccttcagcc agcaagcggt caacctcaac ccgggtggta tgcatctcaa ctctgaaatt
     8341 gaacgcggga ggctggcttc ggtctaaccc cggatatatc tcttcaaacg gcgcgggcag
     8401 cggccaggtt ggcaggggct cgggcggcaa atcctggggg aagtattgcg ccagcagcgc
     8461 ccggctgagt tccaccccca caatggacgc tgtggtttcg ttgattgtcc gcatttcgcc
     8521 gctggcaaaa taacccatgc ccagcgggtg cagtgtcaaa taattatgcg tccattcatg
     8581 cgccaccact tccggcagcc aggtaatgct gctggtagcc atcaccatgg tggggtaaac
     8641 cccaattcct cccactggct caattaacgc tgaaacatta agctcttttt caatcctttg
     8701 ctctagctcg gtgatttctg gcatggtcat gtcaggcagc agcgaaatat catagtcctg
     8761 ccgaatgaca tcccgcggcg agatgatgag cgcataaggc agatccgtca catgatacag
     8821 cggtggcgga actggcgtca gtccctcctc caacagaata gcgttaagct gggcctggat
     8881 aatagattcc gccagtggcc ccagttgctt aagttctgct tgcaactgca gttgatcgcg
     8941 caccagcggc ttcgatgcaa tggtcgggtc tgccacagtg ggggaggtgt acagccattc
     9001 aatatctgcc tttagcttgt tcagcttggc aatctttgcc aggtaatcct tcaccaacgc
     9061 ggcctgttca gcttcgctga gatagctggg cgcgtccaac gctgcctgcg caaacttgat
     9121 ccgcagtgca tccagcatcc agacactgaa atcaaactca tacgcccgcg taaatcgtcg
     9181 caaatgttcg tcggcattgg tcagcagcag tgtgctgccc tgtaccagcc acaggaataa
     9241 aaacccaatc accagtaatc gattgaatcc acgcgcttgt ttcataatga agggatttta
     9301 gcagattatg ggccgatttt acataattct tacgaaatac ctgctacaat cgctcacgcg
     9361 ccggggcaat cggtgctatt attgccctgc ataaatttta cagatcggta tgagtgagga
     9421 gtggcatgaa aagaaagact attgtgacaa ttggaataat cttggccctg gtggtggcgg
     9481 ggtacttctt agtccggtac ttgcaacagc agaaggcctc tgctggttcg gaatatcaaa
     9541 ccgccgtggt cgagcgaggc agcctgacgg cgattgtcgg tgccaccggc accgtccatg
     9601 ccaaccagtc cgcaaccctt gcctgggaaa cttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnggtgcca ccggcaccgt ccatgccaac cagtccgcaa cccttgcctg
     9781 ggaaacttca ggccgcgtgt ccacaattga cgttgctgtg gatgatcagg tcaccgccga
     9841 ccaggttctt gccaccctgg ctcaagattc gctgtcgcaa tccatcatcc ttgccgaggc
     9901 tgatctaatc gccgcccgcc gctcgctgga taatttgctc aattccaaca ccgcagccgc
     9961 ccaggcgcag cttgccctcg tcaacgccca ggaatccttt gatgatgcca gtcaatcccg
    10021 cgccaattac gattacaacc tctacacggc tgataccatc gagattgccc gcaccactta
    10081 ctacctcgcc ctggacaaaa tcaataagta cgaagaaatt tacgagaatt ttaaagataa
    10141 gcccgaagac gaccccctgc gcgcccaggc ggccaataac ctggccaatg cccgccaaaa
    10201 tgccgatcag gcctatgata atctgaatta cctcctgggc ggcccggatc cctatgatat
    10261 ttccgtcgcc gatgccaata tcgccttagc cgaagcccgc ctgaccgatg cccagcggga
    10321 atgggatcgc ctgaagaacg gccccgaccc cgacgacgtc cgatccgccg aagtgcgcat
    10381 agccgcactc gaggccgccc tggattcgaa gaacctgcgg gcgccttttg ccgccaccgt
    10441 taccgaggtc aaatcactcc ccggcgatat ggtgaacccc ggtaccgtct ctttccgcat
    10501 tgacgacctc agccatttgc tggtcgacgt tgatattccc gaggtggaca tcaaccgcat
    10561 ccagattggt caggcggtta acctctcatt tgatgccatt actgaccgcc agtacaccgg
    10621 cgaagtgatt gaagttgctc gcgtgggttc cacagcgcag ggtgtggtta atttcaaagt
    10681 gactattgaa ttgaccgacg ccgatgaggc cgttcgcccc ggcatgacag ctgcagtcaa
    10741 tatcattgtt gaacagttgg atgatgtgct gctggtaccc aatcgtgcgg tgcgctccag
    10801 cggcggtcag cgggtggttt atgttcttag gctgggcatc ccaacccctg tgccggttga
    10861 aattggcgcc acctccgatc tggtcagcga actgctgccc ggagaggtca aagaaggaga
    10921 tgtcctggtg ctcaacccgc ccacccagat tatccccgct gatggaatgt ttgggagata
    10981 gcccccatga ccactgaaga tacggtcatt gtggctactg gcctgaccaa agtctataag
    11041 atgggcgagg tcgaggtcca cgccttgcgg ggcgctaatt tacgcatccg ccgcggcgaa
    11101 gtagtcgcca ttatgggccc atcgggctcc ggcaaatcta ctctgatgaa tattctcgga
    11161 tgtttggata ttcccacgtc tggtgaatat tgcctggatg gtgaatgtat ctcccagctc
    11221 cgcgacgatc aattggcgat tatccgcaac cgcaaggtcg gctttgtttt ccagagcttc
    11281 aatttactct cccgtaccac ggcactttcc aacgtcgagc tgccgctgcg ctatgcgggc
    11341 atccccgcgc gtgatcgcct ggatcgagcc agcgccgcgc tcgaagccgt tggcctcgca
    11401 gaccggatgc gtcacaagcc cacagaactg tccggcggtc agcagcagcg tgtggccatt
    11461 gcccgtgccc tggtcaacga ccccgcaatt ctgatggcgg atgaaccgac cggcaacctg
    11521 gattcgaaat cgggcaagga gattatggaa ctcctgctca atctcaatcg tgaacgtcat
    11581 accactttga taattgtgac gcatgaccaa ctggtggggc agcaggcgca gcgcattatt
    11641 cgtatatccg atggtgtggt cgtggaaggt ggcgaatgaa cctcggacag gcaattatcg
    11701 aagcattgga aagcatcagc ggaaacaaac tccgctcagg cctcactatt ttggggatca
    11761 tcatcggcgt tggcgccgtg atcgccatgc tcggggtagg gcagggtgcc caaaacacca
    11821 tcaccggctc catcagtggc attggcagca atctgctctt tgtcttttca ggcaacatgg
    11881 agcaggaagt ccgcaatgaa aaaccattga ccctcagcga tgccgaagcc atggcagacc
    11941 cgtttcaggc cccttcggta gctgcggttg cgcccgtcat tcagggcagc ggagaaatca
    12001 cctttggcgc cgaaactgcc agcaccagcc tctacggcgt cacccccgat tacacggttg
    12061 ttcgcaatta cggtttgatc gaaggcgaat atattgatga gcagcatctg ctcgggcagt
    12121 cctcggttgt gctgcttggc cccgatgtcg ctgaaaaact gttcggtcgc accgatggta
    12181 tcaccggtga gaccgttcgc atcaatggtc tgcccttccg ggttatcggc atcctggaat
    12241 caaagggtgg cggcagcttc ggcagtcagg atgatgtggc cctggtgccg ttctccacgg
    12301 cgcagtcccg gctcatccgc cgctcgcgcg accgtgtcga tgttgttttt gtccaggttg
    12361 ccagcgctga acaggtcagt gacgcccgcg aagaaatcgc ccagattctg cgcacccgtc
    12421 accgtactgc cctgggcgaa gatgatttca ccatcttctc acaggaagat tttgtcgcca
    12481 ccgctcagac catcactggt gtcttcacca ttttccttgg cggcattgcg ggtatttccc
    12541 tgctggttgg cggcatcggc atcatgaaca ttatgctggt ctcggtgacc gagcgaaccc
    12601 gtgaaattgg cctccgtaag gccctgggtg cccgccgccg cgatatcctc atccaattcc
    12661 tgaccgaatc atccctcttg agcctgatcg gcggtgtggt tggcatcggc ctgggctggt
    12721 tgatcgcctt catcgtcggt aaaattgccg ctgccagcgg cacccccttc tacccctcca
    12781 tcggcgttga ttcaattcta ctcgcaacgc tcttctccac cgcggtgggc ttgttctttg
    12841 gtctgtaccc ggccaaccgc gccgctggtc tcgaacccgt cgaagccttg cggtatgagt
    12901 gagcgaatct ttttcttata actcgatcaa aatggcgagg ctgcccagcc tcgccatttt
    12961 ctatagacgg ctgggtgccc attgtcatat caaaacccgg aacagcgaca ggatgaaaca
    13021 aactcgaaaa tcgtgtacaa tggtaagggt gtaagttccc tgttaccatc aaatcaatct
    13081 aataaggaga ccttgtcatg agtggatgga aagtcttgct gaccgacggc ctggaagaaa
    13141 aaggaaaggc cattcttgca caatcagccg aggttgtgga tcgcaccggt atttcagcag
    13201 atgaactgct gcaaattgtg ggtgaatacg atgcagtcat cgtccgcggt cggacgaagg
    13261 tcatccccgc cgtgtttgat gcggcttcgc gtctcaaggt tgttggccgc gccggcgttg
    13321 gtgtggataa catcgatctg aacgccgccc gcgcccacaa tgtgattgtg gtcaattccc
    13381 cgctggctac caccatcgcc gttgccgaag tgaccatggc gctgatgctc tcggttgtgc
    13441 gcgaagttgc ccgcgccgat gccaccatga aagctggcaa gtggatcaag aaagaacttg
    13501 aaggcgcaga actccacgcc aaaaccctgg gcgtcattgg ctttggccgc attggcgctg
    13561 cagtgggtca acgcgccgcc gcctttggca tgaaaatcat cggctacgat cctctcatcc
    13621 ccgccgatga aatccagcgc cgcggtggca aacccgtgac catggacgaa ctgcttgccg
    13681 aagctgacat catcaccatg cacatccccc tgaccgccga cagcaaaaac atgctcaacg
    13741 ccgatgcctt cgcaaaaatg aagcagggcg tctatctggt ttgcgctgcc cgtggcggcg
    13801 tcattgatga agaagccttg ttggcgtctc tggaatccgg caaggttgct ggcgccggcc
    13861 tcgatgtctt cgccaccgaa cccccgggcc tgaccgctct ggtcagccac ccccgtgtgg
    13921 tctgcacgcc gcatattggc gcccaaacgg tcgaagccca ggagcgcgcc gcggtggata
    13981 tttccagtga agttctggct gccctgcgcg gcgatgccct tcgctggcgt gttgcctgat
    14041 ttttggagct ctaaatgaca aaaattgaac aacttgaagc cctgacgcgt gaacttggcg
    14101 ccattcagcg cgcttctgcc gtgctgggct gggatgccca ggtcaacatg cccgctggtg
    14161 gcgctgaaga gcgcggtttt caactgggcg ttctggctga actgtcacac aaacgcttcg
    14221 tcaatgacga aacccacaac ctgattgttg ccgccgaagc cgaagcggcc agcctcgatc
    14281 cagattcgat tcaggcccgc ctggtcaaat actgccgccg tgattacgac aaagccaccc
    14341 gggtgcccgc cgccaaagtt gccgagtttg cacaggttac cgcagtctcg caggaagcct
    14401 gggcccgcgc ccgctccgaa aataactttg ctgctttcct gccccacctc gagcgcgtga
    14461 tcgaactccg ccgcgagtac gcctcttact ttgcccccta cgaccatgtc tatgaccccc
    14521 tcctggacga ctttgaacgc ggcatgaaaa ccgctgaggt tcaggcaatc ttcaaccagc
    14581 tgcgccccgt gcaggtggaa ctggtgcatg ccattgccgc ccgtccggag attgaaaaca
    14641 gcttccttta ccagaattat cctgagcagg ctcagtggga tttcggcgtt gaagttgcca
    14701 cccgcatggg atatgactgg aaccgcggcc gtcaggataa atcggcccat cctttcacca
    14761 ccagcttcgg ccttggagac gtccgcatca ccacccgctt tgataccggg tatgtcggct
    14821 ccgccctctt cagcaccatg cacgaaggcg gccacgcgct ctatgagcag ggctttgacc
    14881 ccgctctgcg cggcctgcct ctggccgatg gcgcttcgat ggcggtccac gaatcgcagt
    14941 cccgcctctg ggaaaatctg gtgggtcgct ctctcgcctt ctggaagttc ttctatcccc
    15001 gcctcaagga atacttcccc acccagttgg caaatgtaga tctccaaacc ttctaccgcg
    15061 gcatcaacaa ggtcaaaccc tcttacatcc gcgtggaatc tgatgaggcc acctacaacc
    15121 tgcacatcat gctccgcctg gagcttgaaa ttgccctcat ggaaggcagc cttaaacccg
    15181 ccgacctgcc cgccgcctgg aacgctcgct tccaggaata cctgggcctc accccaccca
    15241 ccgacaaagt gggcgtcctg caggatgtgc actggtcgca cggtctgatt ggttacttcc
    15301 ccacctatgc cctcggtaat ctggtctcct gccagctttg ggaagtgatc cagcaggcca
    15361 tccccgacct ggaatcccag atcgagcacg gcgaattggg caccctgttg gcctggctgc
    15421 gccagaatgt ccatacccac ggttcaatgt ttgagcctca ggaactggtt cagaagatca
    15481 ccggctcccg cattgatccg cagccctaca tgcgctatct caagaccaaa tttggtcaaa
    15541 tctacgggct gtaaaagctg atcaatcaca tgcctcccgc agatcaacct gcgggaggcg
    15601 aattggaaaa atactcgaat ggcaagaatt ttatttgtag atgatgacag cctgaccctg
    15661 cagttgctgg accaggctac ccgtttgttg gggcacgacc ctatttacga
//
LOCUS       DAOPVS010000037        15662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3831_length_15662_cov_62.927789, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000037 DAOPVS010000000
VERSION     DAOPVS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15662
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3831_length_15662_cov_62.927789"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKL11_04930"
     CDS             complement(<1..581)
                     /locus_tag="PKL11_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNZ12980.1"
                     /translation="MVHFLREKYVARGGPDGGDGGHGGSVILVVNPKLNTLAAFRHQQ
                     RHIADSGAKGARRNQTGKSAPDLLVPVPAGTLVYDDTSGELLGDLVEPGQQLLVAKGG
                     RGGRGNPHFASARHQAPRTAERGEPAEERFLRLELKLIADVGIVGVPNAGKSSLLAAV
                     TNANPKIANYPFTTLEPNLGVAELDDENTLVLA"
     gene            complement(720..2036)
                     /locus_tag="PKL11_04935"
     CDS             complement(720..2036)
                     /locus_tag="PKL11_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNZ12981.1"
                     /translation="MTIRTLIMGAAGRDFHNFNVYFRDNKEYQVVAFTATQIPNIEGR
                     RYPAALAGSLYPEGIPIYPESELVKLINDLKVEQVIFAYSDVSHETIMHKASLVLATG
                     ADFRLMGQKSTQVKSTKPVVSICAVRTGAGKSQTTRRVSMILRAMGYRVAAIRHPMPY
                     GDLVAQKVQRFATYADLDKHECTIEEREEYEPHIDNGVIVYAGVDYEAILRQAEQEVD
                     IVLWDGGNNDFPFYVPDLAIVVADPHRPGHEYLYHPGETNVRAADVFVINKVDTAAPD
                     KVIAVRETLHRLNPDALVIEGASPLFVDNPEAIRGKRVLVIEDGPTLTHGEMAYGAGW
                     VAARRFGAAEIVDPRPFAVGTITETYQKYPGTGQILPAMGYGEQQMKDLETTINNADV
                     DLVISATPIDLTRIIKINKPMQRVRYELQEIGQPTLEEILKAKFGK"
     gene            complement(2074..3837)
                     /gene="argS"
                     /locus_tag="PKL11_04940"
     CDS             complement(2074..3837)
                     /gene="argS"
                     /locus_tag="PKL11_04940"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNZ12982.1"
                     /translation="MFEHEQQQMEARILAWCAENGLPEPTLQWTWIPFSGQWGISTSF
                     FQLAAQEARSGKRVIVPQRAAELAEAVAAHLGTPAGFDRIEAVKGYLNLYFPPQDYTR
                     RVVDAVLDAGASFGRGERLGQRMMVEFSHPNTHKAFHVGHLRGAILGDALARLLDAAG
                     YDVIRANYPGDMGLHVMKWLWNYAKHHHGERPGADITRWMGDLYAEANRRLEENPELE
                     AEVRALYVRWDQRDPEVVALWEETRQWSLDGFNAMYAQLGIPFDVYYFNSQMEQPGKV
                     IVDELIRRKIAQDERPDGAVIVKLDELLGEKGEKYRVLVVLRSDGTALYATEDLALAT
                     RKFADYPDMVKCIYVVDVRQSQHFTQVFKTLEIAGRDWAKNLQHLPYGLVSLPGNVVM
                     ASREGTVVLLEDLIREATRRALDIVKVKNPDLSAEAQQSVAQAVAIGAIRYPMISREN
                     AKDVTFDWESALNFDGQAAPYIQYAQVRANSILRKAGGSVPPALTPTHELSRQEIALV
                     DLIARFPREVQRAAAELSPMVIANLTFELAQAFNDFYNQCPVLQVEEPVRAARLRLTA
                     ATRQAIINGLALLGITAPQAM"
     gene            complement(3945..4211)
                     /gene="rpsT"
                     /locus_tag="PKL11_04945"
     CDS             complement(3945..4211)
                     /gene="rpsT"
                     /locus_tag="PKL11_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNZ12983.1"
                     /translation="MANIKSQIKRNRQNDVRRLRNRVYRGSARTFVAKARRSMESSDP
                     EAARVAIQEAVSALDKAAQQGVIHKNNAARRKSRLMKHLASLAK"
     gene            complement(4403..6208)
                     /locus_tag="PKL11_04950"
     CDS             complement(4403..6208)
                     /locus_tag="PKL11_04950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNZ12984.1"
                     /translation="MSAKTPPRWDLSNVYPGLQSAEFAADFEKAKAQTADLAAFLTEA
                     EVLDAKTDPALLTEKVNQLLEKFNDLLVRVGTMFAYINSFVATDSFNTEALRKLSEME
                     IASVELEKLGTRAQAWIGKLGPALPVITSKPGAAREHAFWLNETAEQSRYLMSPAEEA
                     IATELNLSGANAWGKLQRTVTSQLSMDFELDGKVQSLSMPAIINLRGHPDEDVRRRAY
                     ETELKAWQTVREPVAAALNGIKGTVNTLNHRRGRTDALHAALDINRIDRATLEAMLTA
                     MRESLPMFQRYFQAKAKRLGKAKLAWWDLFAPAGRSDRTYSFEEARKFVLDNFGHFSP
                     DMAAFAERAFNNNWIDAEQRTGKVAGAFCMDVPAVKESRILLSFDGSLDVVSTLAHEL
                     GHGFHADCMFKAGTTDLQRRTPMTMAETASIMCETIVSNAVLENAADDDERLAILEAA
                     LIGDSQVVVDIYSRYLFEKEVFERREQAELSADELCEIMERCQKAAYGEGLDERYLHP
                     YMWVWKPHYYEASLSFYNFPYTFGLLFGTGLYAIYQQRGADFVPEYKALLASTGMGTA
                     AELAGRFGIDIRTPDFWRGSLGIIEKRLERYLALL"
     gene            complement(6264..6758)
                     /gene="rnhA"
                     /locus_tag="PKL11_04955"
     CDS             complement(6264..6758)
                     /gene="rnhA"
                     /locus_tag="PKL11_04955"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNZ12985.1"
                     /translation="MTAQPAQKTPDSIVILYTDGACSGNPGPGGYAAILKYGGQQRIL
                     SGGFRLTTNNRMELMAVIAGLEALTRPCRVRVFTDSQYVAQAMERGWARRWRANGWMR
                     NRKEPALNADLWERLLKLCETHTVEFAWVRGHASDPDNAACDQLAVAAAAQPNLPPDP
                     GFKV"
     gene            6809..7438
                     /locus_tag="PKL11_04960"
     CDS             6809..7438
                     /locus_tag="PKL11_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12986.1"
                     /translation="MDENESTSTRSDDTLSVSEGLRSGGQLPVTVPPKTDLGRRILTW
                     LAVGGACLLIGALAVYFGFYVPGQKALKTAQDDLATLQQTLTDTQAELATAESDLAAA
                     QTNAAAAQTELATLQAAVPLLRVQNDVLSARLALVGQDTLTARQMLGLAETDMAALVE
                     VLKTTDPEVAGAIQQRLSDAAAAFARNDITDTSSELRILSENLAILGQR"
     gene            7606..8799
                     /locus_tag="PKL11_04965"
     CDS             7606..8799
                     /locus_tag="PKL11_04965"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12987.1"
                     /translation="MKIIERALFPVLKRALSDQRIVVITGMRRVGKTTTLRWLLEQVP
                     SQNKYFMDLERLDQRRVFQEVNYELVLNYFRNLGLDPAQQMTIAIDEIQYAPNLPSIV
                     KYLHDHDNIKFLLSGSSSYYLKNLFNESMAGRKVVYEMFPLGFGEFLDFRGISYRRRS
                     TLNEMRFDPLEYERLKGYYDDFVNFGGLPDVVLEPRLEARREILQDIFSSYINLDVQT
                     LADFRKIGELMQLLKVLAFRIGNKLDYLKLSQIVGISRPTLSEYLEFLEKTYVIFQLP
                     AYTNPDRTVALGKKLYFRDNGIASILGHPGEGALFENAVFNQLKPYGELNYLTRESQF
                     EVDFVLTPANQDPIGLEVKYHPVASDDVKLKRVAQRSQIQKSWLIGRVATPSFTDFIW
                     GGLIF"
     gene            complement(8875..9978)
                     /locus_tag="PKL11_04970"
     CDS             complement(8875..9978)
                     /locus_tag="PKL11_04970"
                     /inference="COORDINATES: protein motif:HMM:NF037526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12988.1"
                     /translation="MFTDQNLRELVAYTAPQPVLSVYLNTNPSEGNADAYRLRLRNLL
                     KMVDLPQDVAAVEQYFNHQYDWSGRAVAVFSCAPQGFFRAYPLSMPVRDWIHMSDRPS
                     VRMLAGLADSYGGYGVALVDKQGARLFFFHMGDLHEQEGTVGEEVRHTKRGGASSMPG
                     RRGGTAGQTRTVEETVERNMKDAAEFAVRFFEENHVRRVLIGGTDENVAMFRGLLPKA
                     WQSLVVGVFTMGMGASKTEVLNRALEIGRSAELEREKRLVEQLITQTAKLQNAVTGLS
                     ETLATVNDGRVQLLIVSEGFRKAAYRCKNCGVLTIAQPDATCAVCGGEFQKFPDGVDL
                     AMSAVMRQGGEVEIVTPIPALDEAGKIGALLRY"
     gene            complement(10019..11197)
                     /locus_tag="PKL11_04975"
     CDS             complement(10019..11197)
                     /locus_tag="PKL11_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ12989.1"
                     /translation="MKKYAAVLFIAVLLLTTLACVLPTIPFLDRSEEEAPVTSSTPVV
                     IVPTVPSPILPTSFTDLSERDALLTALYQNVNPGVVTIINYNDLGGGSGSGFVFDRDG
                     HIVTNYHVIEGADLLEVDFPSGLKVWADLVGTDLDSDLAVLKVDVPADQLRPLALADP
                     ATIMVGQTVIAIGNPFSLNSTMTIGIISAKGRVLDSLRESGTGGYFSAGDMIQTDAAI
                     NPGNSGGPLLNLNGEVVGINRAIYTEATNITEQPVNSGIGFAISVSIVRNVVPEIIAK
                     GFYDYPYIGITSREQLSLQEIEALGLPQTTGAYVIDVADGGPADKAGIQAGQTPTEFA
                     GLESGGDLIIAIDGQPVNVFGDLIAYLVVNKQPGDQVTLTILRDGETKEVTVTLAKRP
                     "
     gene            complement(11243..12136)
                     /locus_tag="PKL11_04980"
     CDS             complement(11243..12136)
                     /locus_tag="PKL11_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNZ12990.1"
                     /translation="MEYKDYYKTLGVARNASAEEIRKAYRKLAMKYHPDRNPGNRQAE
                     EQFKEINEANEVLGDPEKRKRYDSLGESYSQWQQSGGTPGSFDWGAWQSGRGISQEEF
                     NAVFGNFSDFFSAIFGGMPPMGRSGRTGTRSARPTQQYEQPLQISFDEAFHGSERILQ
                     MDNRRIQVKIPAGARTGTKLRVAGAAPNVDLYLVIEVSPHPRYERKDDDLYTDAEIDL
                     YTAVLGGQAQVETPLGRVALTIPAGTQPGQLFRLGGRGMPHLRDPKSAGDLYVRARVQ
                     LPQRLTAAQKALFEQLRTSAS"
     gene            12281..13936
                     /locus_tag="PKL11_04985"
     CDS             12281..13936
                     /locus_tag="PKL11_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ12991.1"
                     /translation="MATVQDRILIVESDPLISDLIGRQTLQAAGYQVFVVGDANAAIA
                     KAVQLVPDVILANLHLSGLTGKDLLVALNSQNIETPVIMIAPKGAESDVIQTFRLGAA
                     DYILWPAREPEIIQVVERTIKQVRERRERERLSKQLQQTNQELQNRVRELTTIFSIGK
                     AVISVTDQSVLFEKILDGAVKVSQADMGWFLMRDEASKLFLLAAQRNLPPTVSARVGQ
                     PFDDGVSSLVAMSGELLTLHGDPIRRFKIAALGQSTLIVPVRVQKQVIGLLVLVRRQP
                     VPFNGSEQNLMEAMADYASISLVNSQLFRTVEQRARSSQMLADSAQTREKIMNEVLSS
                     VKRELNPPITSARDALDKITKDPTLRWNPTQRQTLTDLQDALVTLSRTSEAILPLPVG
                     QFQQGGMRVNLNDLIREAVNRYLPFAQHNNVTLVAGLPLDPVMVQGDAALLTQALHGL
                     LSNGIKYCHPGGQVAIRLEVSREGQAHIAVTDAGSGMDPAHTARVFEPRPQVEAPGPR
                     RFGGLGVGLPLVKTIITQQNGSVWAESKLGQGSTFHVLLPIIK"
     gene            13954..>15662
                     /locus_tag="PKL11_04990"
     CDS             13954..>15662
                     /locus_tag="PKL11_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNZ12992.1"
                     /translation="MPEIKQPKTVAIITADLISGAFIEETLKADGFSVSRYNDLASAW
                     QVLPAAPPDALIVCEKLPDGAALDFLPPLLRRQPALPTLLLAERESAALLRAALTAGV
                     TDCLFKPLAADTLLTTLRGAMERSARLRDWGLLQARRTTDRLRARLDGLETLTRLGQA
                     ITSTLDIDGVLTAIVEAAVELTGAEEGSLLLLDSTTGELYMRAAKNFDQNFVRTFRLA
                     ISDTLAGSVLRSGQPVVLDDSTPTKIKTSYLVQRLAYVPLKLHGSIIGVLGVDNRTQK
                     SLNRDHTVKVLLALAEYAVIALENSRLYNEMETRRRELESILTGIQDGVMVFGADQRL
                     IMLNPAARAVLKLGDENLAGRLASELILDNDLLHLVESAGQTLSNVTEFETDGRSWSV
                     HVNTIPDVGVALLLHDITHLKKMDKIKSEFVHTVSHDLRSPLTAILGYVELIDRAGPV
                     TDLQRSFIQRVQASVQNITKLVNDLVSLGQVEAGFDTQKELVYLDQIVAYVSEQFQKQ
                     LQAKEQNLQITLPDPCPPISANPVQIRQMFEHLLDNAIKYTPNAGRIAIRGEVEEDQI
                     IVQV"
BASE COUNT         3231 a         4822 c         4160 g         3449 t
ORIGIN      
        1 tctgccagca ccagtgtatt ttcgtcatcc agttcagcca cgcccaggtt aggttccagc
       61 gtggtgaacg ggtaattggc aatcttgggg ttggcattgg tcaccgccgc cagcaggctc
      121 gatttgcccg cattgggaac acccacaatg cccacatcgg caattaattt caactccaaa
      181 cgcaggaagc gctcttcagc cggttcgcct cgttcagccg tccggggggc ctgatgcctg
      241 gcgctggcaa aatgcgggtt gccgcgtccg ccgcgcccgc ctttagccac cagtaactgc
      301 tggcctggct ctaccaggtc acccagcagt tcaccgctgg tatcgtcata caccagcgtg
      361 cccgctggca ctggcaccag caaatcaggc gccgatttgc ccgtctgatt cctgcgcgcg
      421 cccttggcgc cgctgtctgc aatgtgccgc tgttgatgcc gaaacgcagc cagagtgttc
      481 agtttggggt tcaccaccag aatcacactg ccgccgtgac cgccatcgcc cccatcgggg
      541 ccgccgcgtg ccacatactt ctcccggagg aaatggacca taccatcgcc acccttgcca
      601 gactgaacat atattttcgc ttcgtcaata aacataatcc gctacacctt gctgtgagaa
      661 gcgtgagtcg taagcaggcc gcgcccgctt acgactcacg ttgaatgtct tacatcgaac
      721 tacttgccga acttggcctt caggatttct tccagcgtgg gctggccgat ctcctgcagt
      781 tcatagcgca cgcgctgcat cggcttgttg atcttgatga tgcgggtcag gtcaatcggg
      841 gtcgccgaga taaccaggtc cacatccgca ttgttgatgg tggtttccag gtctttcatc
      901 tgctgttcgc cataacccat ggcgggcaga atctggccgg taccggggta cttctgatag
      961 gtttcggtga tggtgccgac cgcgaacggg cgggggtcca caatttcggc ggcgccgaag
     1021 cggcgggcag caacccaacc cgcaccgtag gccatttcac cgtgggtcag cgtggggccg
     1081 tcctcgatca ccagcacgcg cttgccgcgg atggcttcgg ggttatccac aaacaggggg
     1141 gaggcgcctt cgatgaccag cgcatccggg ttcaggcggt gcagggtctc gcgcacagcg
     1201 atgaccttgt cgggggccgc ggtatcaact ttgttgatca caaacacatc cgccgcgcgc
     1261 acattggtct cgcccgggtg atacagatat tcgtgaccgg ggcggtgcgg gtcagcgacc
     1321 acaatcgcca ggtccggaac gtagaacggg aagtcattat tgccgccgtc ccacaggacg
     1381 atgtcaactt cctgctcagc ctggcgcagg atggcttcgt aatccacacc ggcatacacg
     1441 atcacgccgt tgtcaatgtg aggttcgtat tcttcgcgtt cttcaatcgt gcattcgtgc
     1501 ttgtccaggt cggcataggt cgcaaagcgc tgcaccttct gggcaaccag gtcgccgtag
     1561 ggcatggggt ggcggatggc agccacacgg taacccatgg cgcgcaggat catggaaacg
     1621 cggcgggtgg tctggctctt gccagcgcca gtgcgcacgg cgcagatcga aaccacaggc
     1681 ttggtcgatt tcacctgggt cgatttctga cccatcaggc ggaagtcggc gccggtagcc
     1741 agcaccagcg aagccttgtg catgatggtc tcatgcgaca cgtcactgta ggcgaagata
     1801 acctgctcaa ccttcagatc gttgatcagt tttaccagtt cggattcggg gtagatcgga
     1861 ataccttcgg ggtacagcga accggccagc gcagccgggt agcggcggcc ttcaatattg
     1921 gggatctgcg ttgcagtaaa agccacaacc tggtattcct tgttatcgcg gaaatacaca
     1981 ttgaagttgt ggaaatcgcg cccggcggcg cccatgatga gagtacggat ggtcatacag
     2041 ctgctccttt tgtgaaatat gttggatacg ctttcacatc gcttgtgggg ctgtgatgcc
     2101 cagcaaagcc agcccattga tgatggcctg gcgggttgcc gcagtcaggc gcaggcgcgc
     2161 cgcgcgcacc ggttcttcaa cctgaagcac cgggcattga ttgtaaaaat cgttgaaggc
     2221 ctgcgcaagt tcaaaggtca ggttcgcaat caccatcgga gaaagctccg ccgcagctcg
     2281 ctgcacttcg cgcgggaagc gggcaatcaa atcaaccagc gcaatttcct gtcggctcaa
     2341 ctcgtgggtt ggcgtcagcg ccggcggcac actgccgccc gccttgcgca ggatgctgtt
     2401 ggcccgcacc tgggcatatt gaatatacgg cgccgcctga ccatcaaaat tcagcgccga
     2461 ttcccagtca aaggtgacat ctttcgcatt ctcgcgagaa atcatcgggt agcggatggc
     2521 gccaatcgcc accgcctgcg ccacgctttg ctgcgcctca gccgataggt cggggttctt
     2581 gaccttcaca atgtccagcg cccggcgggt ggcttctcgg atcaaatctt ccagcagcac
     2641 caccgtgccc tcgcgtgatg ccatcaccac gttgcccggc aggctcacca gcccgtaggg
     2701 caggtgctgg agatttttgg cccagtctcg cccggcaatt tccagcgtct taaagacctg
     2761 ggtaaagtgc tgcgactgcc gcacatccac cacataaatg cacttgacca tgtcggggta
     2821 atcggcaaat tttcgggttg ccagcgccag gtcttcggtc gcatacagcg ccgtgccgtc
     2881 tgagcgcagc accaccagca cgcggtactt ctcgcctttt tcacccagca gttcgtccag
     2941 cttgacgatc accgccccat cagggcgctc gtcctgggca attttgcggc ggatcagttc
     3001 atccacaatc accttgccag gctgctccat ctgactgttg aaataataca catcaaacgg
     3061 aatgcccagt tgggcgtaca tggcattgaa cccatccagc gaccactgcc gggtctcttc
     3121 ccacagcgcc accacttcgg ggtcgcgctg gtcccagcgc acatacagcg cccgcacctc
     3181 ggcttccagt tcggggtttt cttccagccg gcggttggcc tcggcgtaca ggtcgcccat
     3241 ccagcgggtg atatccgccc ccgggcgctc gccgtggtga tgtttggcgt aattccacag
     3301 ccacttcatc acatgcagcc ccatatcgcc cggatagttc gcccggatca cgtcataacc
     3361 tgccgcgtcc agcaggcgcg ccagggcgtc gcccagaatg gcgccgcgca aatgccccac
     3421 atggaaagcc ttgtgggtgt tggggtgcga aaattccacc atcatgcgct gccccaggcg
     3481 ctcgccgcgc ccaaagctgg cgcctgcgtc cagcactgca tccaccaccc ggcgggtata
     3541 atcctgcggg gggaagtata aattcaggta acccttcacc gcttcgatac ggtcaaagcc
     3601 agccggggtg cccaggtgcg ctgccacggc ttctgccagt tcagccgccc gctgcggcac
     3661 aatcacccgc ttgcccgaac gcgcctcttg agccgccagt tggaagaagg acgtgctgat
     3721 gccccactgt ccgctgaatg gaatccaggt ccactgcaaa gtgggctccg gcaggccgtt
     3781 ttcggcgcac caggccagaa tgcgggcttc catttgctgc tgctcgtgtt caaacatgtg
     3841 cgttctctcg ttaccctaaa agcgaaagcg ggagtaacag gcactcccgc cgcttgtaaa
     3901 aaattgttcg tccccgcagg ccttgccggg gggcacgcaa tcgcttactt agccagagaa
     3961 gccaagtgct tcatcaaccg gctcttgcga cgggcggcat tattcttatg gatgacaccc
     4021 tgctgagcgg ccttatccag cgcgctcaca gcttcctgaa tggccacgcg ggcggcttcg
     4081 ggatcactgg attccatgct gcggcgggct ttggcaacga aggtacgggc agagccgcga
     4141 taaacacggt tgcgcaggcg gcgtacgtcg ttctggcgat ttcgcttaat ttgagacttg
     4201 atattagcca acggtcttcc tccaaaaaat aactttctct aatgcgaagg cgaattttac
     4261 ccgaaactgc cccatttgtc cagcatttat cctggcagaa tctcacattc ttctggagca
     4321 atcttcctga taatccctgc cgtcaggtgc gccagcagca cacctgacgg caggacaatt
     4381 cttgaaatct gatgcgcctg gtttacagca gcgccaggta gcgttccagc cgcttttcga
     4441 tgatgcccag gctgccgcgc cagaaatcgg gcgtgcggat gtcaattcca aagcgcccgg
     4501 ctaattcggc ggcggtgccc atgcccgtgc ttgccagcag cgctttgtat tccggcacaa
     4561 aatctgcgcc gcgctgctgg tagatggcgt agagtccggt gccaaagagc agcccaaacg
     4621 tgtacgggaa gttgtagaac gacaggctgg cttcataata atgcggcttc caaacccaca
     4681 tgtaggggtg caggtagcgc tcgtccagcc cttcgccgta ggcagccttc tggcagcgct
     4741 ccatgatctc gcacagctca tctgccgaaa gttcggcctg ttcgcggcgc tcaaacactt
     4801 ccttctcaaa caggtagcgt gaatagatat ccaccaccac ctgcgaatcg ccaatcagcg
     4861 cagcttccag aattgccagc cgctcatcgt catcggcggc attttccagc accgcgttgc
     4921 tcacaatcgt ctcgcacatg atcgaggccg tttccgccat cgtcatcggg gtgcggcgct
     4981 gcagatcggt ggtgcccgcc ttgaacatgc agtctgcatg gaagccgtgc cccagttcgt
     5041 gcgccagtgt gctgaccaca tccagcgagc cgtcaaagct cagcagaatg cgtgattcct
     5101 tcaccgccgg cacatccatg cagaacgcgc ccgccacttt gccagtgcgc tgttcggcat
     5161 caatccagtt gttgttgaag gcgcgctctg caaacgcagc catgtcgggc gagaaatgcc
     5221 caaaattatc cagcacaaat ttgcgggctt cttcaaagct gtaggtacgg tcactgcgcc
     5281 cggccggggc aaacaggtcc caccatgcca gtttggcctt gcccagccgt ttggccttgg
     5341 cctggaagta ccgctggaac atgggcaggc tctcgcgcat cgcggtcagc atggcttcca
     5401 gggttgcccg gtcaatccgg ttgatgtcca gcgccgcgtg cagcgcatcg gtgcgcccac
     5461 gtcggtgatt cagggtgttc accgtacctt tgatgccgtt cagcgccgcg gcaaccggct
     5521 cgcgcacggt ttgccaggct ttcagctcgg tttcataggc ccgccgccgc acatcttcat
     5581 ccggatgccc gcgtagattg atgatcgccg gcatcgaaag cgactgcacc ttgccatcca
     5641 gctcaaaatc catgctcaac tgggaggtga ccgtccgctg cagcttgccc caggcattgg
     5701 caccgctcag gttcagctca gtcgcaatcg cctcttcagc ggggctcatc agatagcggc
     5761 tctgctcagc ggtttcattc aaccagaagg cgtgctcgcg ggcagcccct ggcttgctgg
     5821 tgatgaccgg tagcgccggg cccagcttgc cgatccaggc ctgggcgcgg gtgcccagtt
     5881 tttccagctc cacgctggca atttccatct cagagagctt ccgcagcgcc tcggtgttga
     5941 atgaatcggt cgcgacgaag gaattaatat aggcaaacat ggttccaacg cgcaccaaca
     6001 ggtcgttgaa cttttccagc aactggttca ctttttcggt cagcagcgcc ggatccgttt
     6061 tggcgtccag tacttcagcc tcggtcaaaa aagcggccag gtcagcggtc tgggctttag
     6121 ctttttcaaa atcagcggca aactccgccg attgcaatcc cggatagaca ttggaaagat
     6181 cccaacgtgg aggggttttg gcactcattc aagctcctta tgatcgtgaa acgaataaat
     6241 gtaatgctat tataccgcga cccctaaact ttgaatcccg ggtcgggcgg caggttaggt
     6301 tgagccgccg ccgccactgc cagttggtcg caggcagcgt tgtccgggtc actggcatgg
     6361 ccgcgcaccc aggcaaattc gacggtgtgg gtttcgcata gctttagcag ccgctcccac
     6421 aggtcggcat tcagcgccgg ttctttccgg ttgcgcatcc aaccgtttgc ccgccagcgt
     6481 cgcgcccagc cccgctccat cgcctgcgcc acatactgcg aatcggtgaa cacccgcacc
     6541 cggcaggggc gggtgagtgc ctccagcccg gcaatcacgg ccatcaattc catccggttg
     6601 ttggtcgtca ggcggaatcc cccactgagg atgcgctgct gccccccata ctttaatata
     6661 gccgcatatc cgccaggtcc cgggttgccc gagcaggccc catcggtgta caggatcacg
     6721 attgaatcag gtgttttttg agcaggttgt gcagtcaatt ttagagtata cttttctata
     6781 tccaatcatc gtatgtcagg agtgtatcat ggacgagaat gaatcgacaa gcacccgctc
     6841 agacgatacc ctgtctgttt cagagggttt gcgctccggg ggacagctcc cggtgaccgt
     6901 gccgcccaaa actgatctgg gccgccgcat cttgacctgg ctggcggtcg gcggcgcctg
     6961 cctgctcatc ggcgcgctgg ccgtctattt tggcttttat gttcccggcc aaaaagccct
     7021 caaaactgct caggatgatt tggccaccct tcagcaaact ctgacggata cccaggccga
     7081 actcgccacc gccgaatccg acctggctgc cgcccagacc aacgcggccg cagcccaaac
     7141 cgaactggcc accctgcagg cggctgtgcc cctgctgcgc gtgcagaatg atgtcctctc
     7201 tgcccggttg gccctggtcg gtcaagacac gctgaccgcc cgccagatgc ttggcctcgc
     7261 cgaaacggac atggccgcac tggtagaagt cctcaagacc accgaccccg aagtcgccgg
     7321 tgccatccag cagcgcctgt ctgatgctgc cgccgccttt gcccgcaacg acatcaccga
     7381 caccagcagc gaactgcgca tcctcagcga aaaccttgcc atcctcggcc agcgctgagc
     7441 ttgccataca tcaccatcga accaccggcg ggcaccatgc ccgccggttt gtattaccgg
     7501 taacactcca ataatcagga cttttgatct attgtacttt acaaatatag acatttttgg
     7561 taattgctac tttacaaata tactataata ttgtaaaata cgaatatgaa aattattgaa
     7621 cgtgctctct ttccggtgct taagcgggcc ctgtcagatc aaagaattgt tgtcatcact
     7681 ggcatgcgtc gggttggtaa aacaacaacc ctgcgctggc tgctggaaca ggttccatct
     7741 cagaataaat attttatgga ccttgagcgt ttggatcaac gtcgggtttt tcaagaggtt
     7801 aactacgaac tggttttgaa ctacttccgc aatttgggtc tggaccccgc tcaacagatg
     7861 acgatagcta tcgatgaaat tcaatacgct cccaatctgc caagtattgt taagtacttg
     7921 catgatcacg ataacatcaa atttttgctt tcaggatcaa gttcgtatta cttaaagaat
     7981 ttattcaacg aatcaatggc ggggcgtaaa gtggtgtatg aaatgttccc gcttggcttt
     8041 ggcgagtttt tagatttcag gggaatctct tatcgccgca gatcaacatt gaacgagatg
     8101 cggtttgacc cccttgaata tgaacgctta aaaggctact atgatgattt tgtgaacttt
     8161 ggcggcttgc ccgatgttgt cttagagccg cgcctggaag cgagacgtga aattttgcag
     8221 gatatcttct cgtcatatat caatctggat gtacagaccc tggctgactt ccgaaaaatc
     8281 ggagagctta tgcagctcct taaggtattg gcctttcgga tcggtaacaa attagattat
     8341 ctcaaactct cccaaattgt gggaatttca cgcccaactt tatccgaata tttggaattt
     8401 ctggaaaaaa cgtacgttat tttccaactc cccgcctata ccaatccaga tcgcaccgtt
     8461 gctttaggca aaaagctcta ttttcgtgac aatggaattg caagcatttt ggggcatcca
     8521 ggagaaggcg ctcttttcga aaatgcagtt ttcaatcaat taaaacccta tggcgaattg
     8581 aattacctga ccagggaaag ccaatttgag gtggattttg ttctgactcc cgccaaccag
     8641 gatccaattg gccttgaagt taaatatcac ccggttgctt ctgatgatgt caaactgaaa
     8701 cgtgttgccc agagaagtca aatccaaaaa tcatggctga ttggtcgggt agcaacgccc
     8761 agctttaccg attttatctg gggcggattg atattttgaa cccttcggtt ccaggtggtt
     8821 agcaggataa aaaagaggcg gccaatcggc cgcctcctct tctaccatca atcattagta
     8881 gcgcagcagc gccccaatct tcccggcctc atccagcgcc ggaatgggcg taacaatttc
     8941 cacctcgccc ccctggcgca tcactgcgct catcgccaga tccaccccat cggggaactt
     9001 ttggaactcg cccccgcaaa cggcgcaggt cgcatcgggt tgggcaatcg tcagcacgcc
     9061 gcagttcttg cagcggtacg ccgccttgcg aaagccttcc gaaacaatca gcagctgcac
     9121 ccgcccgtca ttcaccgtgg ccagcgtctc cgacagcccg gtgaccgcgt tctgcagctt
     9181 ggcagtttgc gtaatcagtt gttccaccag ccgcttttca cgctccagtt cggcgctgcg
     9241 gccaatctcc agggcgcggt tcagcacctc cgttttgctg gcgcccatcc ccatggtaaa
     9301 cacgcccacc accaggctct gccaggcttt tggcagcagc ccgcggaaca tcgcaacgtt
     9361 ttcgtcggtc ccgccaatca acacgcggcg gacatgattt tcttcaaaga accgcaccgc
     9421 aaattctgcg gcatccttca tattgcgttc cacggtttct tcaaccgtcc gggtttgtcc
     9481 tgctgttccg ccgcggcggc ccggcatgga tgatgccccg ccgcgtttgg tatgccgcac
     9541 ctcttcgccc acggtgcctt cctgctcgtg caggtcgccc atatggaaga agaacagccg
     9601 cgcaccctgc ttatccacca gcgccacccc atagccgccg tagctgtcgg ccaacccggc
     9661 caacatacgc acactggggc ggtcgctcat gtggatccaa tcgcgcaccg gcatactgag
     9721 cggataggcc cggaaaaatc cctgcggcgc acacgagaac acggctaccg cgcgcccgct
     9781 ccagtcgtac tggtgattaa aatactgctc aactgccgcc acatcctgtg gaagatcaac
     9841 catcttcaat agattgcgca gccgcagccg ataagcatct gcattgccct ccgaggggtt
     9901 ggtattgagg tacacactca gtacgggttg aggggctgta taagccacca actcccgcag
     9961 gttttgatct gtgaacatag caacctccgt ggtttgaaac ttgccagaat acaatgccct
    10021 atgggcgttt cgccagggta actgtcacct cctttgtttc cccatcccgc aggatggtca
    10081 gagtgacctg gtcaccgggt tgcttgttca cgaccaggta ggcgatcaaa tcgccaaata
    10141 cattcaccgg ctgtccatca atggcgatga tcagatcgcc accgctttcc agcccggcaa
    10201 attctgtggg ggtttgtccg gcctgtatcc cggccttgtc tgccgggccg ccgtctgcaa
    10261 cgtcaatcac ataagcgccg gtggtctgcg gcaggccaag cgcctcaatc tcttgcagcg
    10321 aaagctgctc gcggctggtg atgccaatat aggggtaatc gtagaaccct ttggcgatga
    10381 tttctggcac cacattgcgc acaattgaaa ccgaaatggc aaaaccgatg ccgctgttca
    10441 ctggctgctc ggtgatattg gtggcctcgg tgtaaatggc ccggttgatg cccaccactt
    10501 caccattcag gttgagcagc ggcccgcccg aattgcccgg gttgatggct gcatctgtct
    10561 ggatcatgtc gcccgccgag aaatatcccc ccgtccccga ctcgcgcagt gaatccagta
    10621 cccgcccttt ggctgaaata atcccaatcg tcatcgtgct gttcaggctg aacgggttgc
    10681 caatggcaat caccgtctgc ccgaccataa tggtcgccgg gtcagccagt gccagcggtc
    10741 gaagctggtc agccggcaca tcaactttca acaccgccag gtcagagtcc aggtcagttc
    10801 ccaccagatc ggcccagacc ttcagcccgg atgggaaatc cacttccagc aggtcagccc
    10861 cttcaatcac atgataattg gtcacaatat ggccgtccct gtcaaacaca aagcccgaac
    10921 cagaaccgcc ccccaaatcg ttgtaattga taatcgtcac cacgccgggg ttcacgtttt
    10981 gatacagagc cgtcagcagg gcatcccgct cgctcaaatc agtaaaactg gtcggcagta
    11041 tgggcgacgg cacggttggc acaatcacca ccggcgttga gctggtaact ggcgcttctt
    11101 cttcagagcg atccagaaac gggatggtgg gcagcacaca cgccagtgtc gtcagcaaca
    11161 atacagcgat gaataagacg gctgcgtatt ttttcatttg tacctctatt gtagcccgaa
    11221 ggccaattaa tcagagtcgt ttttagcttg cagatgtccg caactgctca aataaagctt
    11281 tttgagcggc tgttaaccgc tgcggcagtt gcacccgggc ccgcacatac aggtccccgg
    11341 cagatttggg gtctcgcagg tgcggcattc cccgcccgcc caggcgaaac aattgccccg
    11401 gctgagtccc cgccgggatg gtcagcgcca cccgtccgag cggcgtctct acctgcgcct
    11461 gcccgcccag tacagcggtg tacaggtcaa tttcagcatc agtgtacagg tcatcatcct
    11521 tgcgctcgta ccgggggtgt gggctgacct caatcaccag atagaggtcc acattcggcg
    11581 cagcccccgc cactctgagc tttgtgccgg tccgggcgcc agccggaatc ttgacttgaa
    11641 tccggcggtt gtccatctgt agaatgcgct cactgccgtg aaacgcctca tcaaaactga
    11701 tttgcagcgg ttgttcatac tgctgtgtgg ggcgggcact gcgtgtcccg gtgcgcccgc
    11761 tgcggcccat gggcggcatg ccgccaaaga tggcgctgaa aaagtctgag aagttgccaa
    11821 ataccgcgtt aaactcctcc tgcgatattc cccgcccgct ttgccaggcg ccccagtcaa
    11881 agctgcccgg tgtgccgccg ctctgctgcc actgcgaata agactcgccc agcgaatcat
    11941 aacgcttgcg cttttccgga tcccccagca cttcgttggc ctcattgatt tccttaaact
    12001 gctcttcagc ctgccggttg cccgggtttc ggtcggggtg atatttcatc gccagtttgc
    12061 gataggcttt gcgaatctct tctgcgctgg cgttgcgcgc cacgcccagg gttttgtaat
    12121 aatctttata ctccataatt tgattgtatg cttatgcagc ctgtgaagtc aagtaccctg
    12181 aggccgggtc ttacaagatt cttacaattt caagaaccca acaggactgt aagcccaaat
    12241 tccctcccgc ttatcccttt tccatgctat cataatacct atggcaactg tacaagaccg
    12301 catcctgatt gtcgaaagcg atccgctcat cagcgacctg attgggcggc aaacgctgca
    12361 ggcggcaggg tatcaggtgt ttgtggtggg tgatgccaac gcagccattg ccaaagccgt
    12421 tcaactggtc ccagatgtta ttctggcaaa tctccatctc tccgggttga ccggcaaaga
    12481 cctcctggta gcgctcaatt cacagaacat cgaaaccccc gtcatcatga ttgctcccaa
    12541 gggcgctgag tcggatgtca tccaaacctt ccgccttggt gcggctgact atatcctttg
    12601 gccagcccgc gaacccgaga tcatccaggt ggtcgagcgc accatcaaac aggtgcgtga
    12661 acgccgtgaa cgcgaacggc tttcaaaaca gcttcaacag accaatcaag agctgcaaaa
    12721 ccgggtccgc gaattgacca ccatcttttc aattggcaaa gcggtcatct cggtcaccga
    12781 tcaatcggta ctctttgaaa aaatcctcga tggcgccgtc aaggtcagtc aggccgacat
    12841 gggctggttc ttaatgcggg atgaagcttc caagctcttc ctgctggcag cccagcgcaa
    12901 cctgccgcct acggtcagcg cgcgtgtggg ccagcctttt gatgatggcg tcagctcgct
    12961 ggtagccatg tcaggcgaac tgctcaccct gcatggcgat cccatccggc gcttcaaaat
    13021 cgccgcgctc ggccaatcca cgctgattgt ccccgtgcgc gtccaaaagc aggtcattgg
    13081 cctgctggta ctggtgcgcc gccagccggt gccattcaat ggcagtgaac aaaacctcat
    13141 ggaagcgatg gccgattatg cttccatctc tctggtcaac tcacagctct ttcgcacggt
    13201 tgaacagcgg gcgcgttctt cgcaaatgct ggcagacagc gcccaaaccc gtgaaaagat
    13261 catgaacgag gtcctttcct ctgtcaaacg cgagctgaat ccccccatca cttcagcccg
    13321 tgatgcgctg gataaaatca ccaaagaccc caccctgcgt tggaacccaa cccagcgcca
    13381 gaccctcacc gatttgcagg atgccctggt cacgctgagc cgcacatcag aggccattct
    13441 cccgctgcct gttggacagt ttcagcaggg cggcatgcgg gtcaatttga atgacctgat
    13501 ccgtgaggcg gttaaccgtt atctgccctt tgcccagcat aacaacgtca ccctggttgc
    13561 tggcctgccg ctcgacccgg tcatggtgca gggtgatgct gccttgctga cccaggcgct
    13621 gcatggcctg ctcagcaatg gcatcaaata ctgccacccc ggcgggcagg tcgctatccg
    13681 gctggaggtc tctcgtgaag gccaggccca cattgccgtg actgatgctg gatctggcat
    13741 ggacccggct cataccgcac gggtctttga accgcgtccc caggtcgagg cccccggacc
    13801 gcgccgcttt ggcgggttgg gggttggcct gccgctggtc aaaaccatca tcacccagca
    13861 aaacggctcc gtctgggccg aaagcaagct ggggcagggc agcacgttcc atgtcctcct
    13921 gccaatcatc aagtaacatc taccaggtgt cgaatgcccg agattaaaca accgaaaacg
    13981 gttgccatca ttactgccga tcttatatcc ggcgctttta ttgaagaaac cctcaaagcg
    14041 gatggctttt ccgtttcccg gtataacgat ctggcttcag cctggcaggt actgcccgcg
    14101 gcgccgcccg acgcgctgat tgtctgcgaa aaactgcccg atggcgccgc cctggatttt
    14161 ttgcccccgc tgctgcggcg ccaacctgcg ctgcccaccc tgctgctggc tgagcgcgaa
    14221 tctgccgccc tgctgcgggc agctctgact gccggtgtga ccgactgtct cttcaaaccc
    14281 ctggcagccg atacactgct gaccaccctg cgcggcgcca tggaacgttc agcccgcctg
    14341 cgagattggg gcttgctgca ggcccgccgt accaccgacc gcctgcgcgc ccgcctcgat
    14401 ggattggaga ccttgacccg gctggggcag gccatcacca gcactttgga tattgacggc
    14461 gtgctgaccg ccattgtgga ggccgccgta gagttaaccg gtgccgaaga aggcagcctg
    14521 ctgctgctgg attcgaccac cggcgaactt tatatgcgcg ccgccaaaaa ctttgaccag
    14581 aattttgtcc gcaccttccg cctggccatc agtgacacgc tggcaggttc cgtgctgcgc
    14641 tccggtcagc cggtggtttt ggatgactcc acccccacca aaatcaagac ttcttacctg
    14701 gtgcagcggc tggcctacgt gcccctcaag ctgcatggct caattattgg tgtactgggg
    14761 gtagataacc gcacccaaaa atcgcttaac cgtgaccaca ccgtcaaagt gctgctggcc
    14821 ctggctgaat acgctgtcat tgcgctggaa aattcccgcc tttacaatga gatggaaacc
    14881 cgccgccgtg aactcgaatc cattctgacc ggcatccagg atggcgtgat ggtctttggc
    14941 gccgatcaac gtctcattat gctcaaccct gctgcgcgtg ctgtgctcaa actgggcgat
    15001 gaaaaccttg ccggccgcct ggcctctgaa ctgatcctcg ataacgacct gctgcatctg
    15061 gttgaatccg ccggacagac gctgtccaac gttaccgaat ttgaaaccga tggccgttcc
    15121 tggagcgtcc acgtgaacac cattcccgat gtgggtgtgg ccctgctgct gcacgacatc
    15181 acccacctca agaagatgga caagatcaaa agcgaatttg tccatactgt ctcgcacgat
    15241 ctgcgctctc cgctcacggc cattttgggg tatgtcgagt tgattgatcg cgccggcccc
    15301 gtcaccgatt tgcaacgcag ctttatccag cgtgttcagg ccagcgttca gaacatcacc
    15361 aagctcgtca acgacctggt cagcctgggc caggtagagg ccggttttga tacgcagaaa
    15421 gagctggtct atctggatca aattgtggct tatgtttcag agcagttcca aaaacagctc
    15481 caggccaaag agcaaaacct gcaaatcaca ctgccagatc cttgcccacc aatttcggcc
    15541 aacccggtgc aaatccgcca gatgttcgag catctgctcg acaatgctat taagtacacc
    15601 cccaacgctg gccgcattgc catccgcggc gaagtggaag aagatcaaat tattgtgcag
    15661 gt
//
LOCUS       DAOPVS010000038        15163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_3983_length_15163_cov_60.087967, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000038 DAOPVS010000000
VERSION     DAOPVS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3983_length_15163_cov_60.087967"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..>1591
                     /locus_tag="PKL11_04995"
     CDS             444..>1591
                     /locus_tag="PKL11_04995"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ12993.1"
                     /translation="MEKARTRARRRRIPTTRRERWAWYFYDFGNSAYAAVVLLAVYSA
                     YFKGQVVGGSEGTRLWGVSVGIAMLVVALLSPILGTLADYTASKKKYLFIFSSMAIVF
                     TAALFFVQPGNIFMGMLFFILAEIGYRSGQVFYNALLGDIASPNEMSRVSGNGWAIGS
                     AGGIVCLLVVLPLIVLVGGTFVVRISFLITAAFYLASSIPIFIWLRERAEPQPLPQKT
                     SVLALAFRRLADTFKAIRNYKELVKFIVAFLVFNNGIMMTLDFAAIIGATLFGMTQTQ
                     LIIFVIIVQITSVLGAFLFGKLAGRWSSKYSLIISLVLMIVAVSLLFIVDSLVMFNII
                     GALAGFALTGVQSVSRTMVGQMAPEERSTEFYGFFSVAGQLSAFIGPAL"
     assembly_gap    1592..1691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1692..2408
                     /locus_tag="PKL11_05000"
     CDS             <1692..2408
                     /locus_tag="PKL11_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ12994.1"
                     /translation="ITYPRRVAIRRVLRFFIRLIFSIIADFKIEGRENIPTNGPVLVI
                     ANHFSFLDPVALIGVLPWTIEFVGGTKLPNAPKYVWWLQRLYGVLHVNRGGVSRETLL
                     ASRNVLKQGGILGILPEGGSWATVLRPPRPGTSYLATTVAAPILPVGLDGLTDVFPLK
                     LGRRARVTIRFGKPFGPFYVSDRGETNRTRLDELGHEMMRQISVLIPPERRGHYSDDP
                     AIRAAAQGTEIYPWADQTEL"
     gene            2449..>3086
                     /locus_tag="PKL11_05005"
     CDS             2449..>3086
                     /locus_tag="PKL11_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNZ12995.1"
                     /translation="MNPTPKRFPNNFIWGSATSSYQIEGAWNEDGRGLSIWDTFCRQP
                     GTVYHGANGDVAADHYHRWREDVALMASLGLKAYRFSVAWPRILPEGTGRVNPAGLDF
                     YDRLVDGLLEQGIDPYLTLYHWDLPQILEDRGGWPDRKIVDAFVEYSTVVARRLGDRV
                     KHWITHNEPICTSLLGYFWGIHAPGIQNPASALAAAYHVILSHGAAVQALRSV"
     assembly_gap    3087..3186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3187..3337)
                     /locus_tag="PKL11_05010"
                     /pseudo
     CDS             complement(3187..3337)
                     /locus_tag="PKL11_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258383123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            3765..4835
                     /locus_tag="PKL11_05015"
     CDS             3765..4835
                     /locus_tag="PKL11_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNZ12996.1"
                     /translation="MAFNPLNWLMGLLSLDIGIDLGTANTLVNVRGKGIVINEPSWVT
                     IDKRTRRPLAIGAQAKEMVGRTPANVIAIRPLRDGVISEFEITQAMLGYFIGKAHQQT
                     IVPMPRPRVVVGIPSGATEVEKRAVYDASMAAGARETYLIEEPRAAALGAGLPVSEVR
                     GSMVVDIGGGTTEVAVLAMGGVVVSRSLRVAGDELDQDIIQYIRNKYNLFIGERMAEQ
                     VKMQIGSAYPLPNEKTMTVRGRNLVTGLPETLEISSVEIREAVGSSVQVIVDTIKDAL
                     DECPPEIVSDLIETGICLAGGGAMLQGLMDRLSDELNLRVWVAEDPLTCVARGAGMIL
                     EDLDRYADFLVGLERTGAAPSA"
     gene            4884..5723
                     /gene="mreC"
                     /locus_tag="PKL11_05020"
     CDS             4884..5723
                     /gene="mreC"
                     /locus_tag="PKL11_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNZ12997.1"
                     /translation="MKSPVPRFWQTVVVVLIVVGLMLLAVSGYLGPVLRAAQTPLVAA
                     QRWISSRYLAIYELITSPTDMAALRQQNQDLQNQVSQLQTEVIELEQRLGEAQVLYAL
                     LDFAREQPQNTYVAAAVIGRDPNPFMRYIFIDRGSDSGVLAGMPVVTAQGLIGRVDAV
                     TAQACRVALITDPATVINVTLQSLKADAQLVGSLTGELTLEMIPSDVNLQPGELVLTS
                     GLGGDYPSDVLIGQVVSVRKLETDLFQTATVQSAVDFNNLQAVLVITNFRPVDIAPLI
                     PED"
     gene            5726..6226
                     /locus_tag="PKL11_05025"
     CDS             5726..6226
                     /locus_tag="PKL11_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ12998.1"
                     /translation="MPVLIGLGVIILFSLLQIGVISQLPLIQGRADLMLLILSAWALN
                     ERVEHAWEWTIIAGVLMSLISATPFFLPLFVYLVVTALARALRRRIWQTPLLMLLFIV
                     LIGTLLQHGLSFVSLSIMGSRLPLQDSLTMVTLPSLLLNLLLALPVYTMITDIAGWVY
                     PAELDV"
     gene            6223..8382
                     /gene="mrdA"
                     /locus_tag="PKL11_05030"
     CDS             6223..8382
                     /gene="mrdA"
                     /locus_tag="PKL11_05030"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNZ12999.1"
                     /translation="MSSSNGLAQSHIATWRVVVIYVALALVFSVYSYRLFSLQVLEGE
                     TYRTQAEENRTKIINIQTTRGLIYDRNGYILASNAPSYNVVITPAELPGNPVLVMKAY
                     RELYDFDYTEEAGAVQEVYRKLSDLIDVPVITPNLWDEDGYIQEIQVKNFSPCYSDLG
                     ITEIVIIGDTNAPYSPIEIKCNIDERTAMIIRSKAAELPGVSIAITPVREYPTGWLTS
                     EIIGFLGPIPAAEEDYYESLGLVPNRDKVGYAGVEATLQDILSGTNGQRVVEVDNAGQ
                     ILRDIEAPIDPIPGNNVRLTIDTRLQTAAKEILLDEMEGWNNWFGYERYNNGVVIAMN
                     PQTGEILALVSYPSYENNRMAREIPAYYYNQLISDPRRPLFNHAISAEHPPGSVFKMA
                     AAIGILNEGVVTPEQEIFDAGVIEIEERFTPNDPNPGTREYVCYTYKTTGAGHGDVDY
                     LHGIALSCDVYFYKVGGGYEDEVPEGLGIWRLGEYARALGYGSQTGIELPGEGDGFMP
                     DPTWKRIYKFENWSTGDTYISTIGQGYVTSTPLQVLVSVATLANDGKMMKPTLIREVL
                     DAEGNVVTPFTPVEVWDITKDPLIHVYDENYLRTDELKTVEPWVIDMAQQGMRMVVTE
                     GTASAYFEGFDIPSGGKTGTAEYCDDIASARDICKPGAWPAHAWYVGYAPFDNPEIAI
                     VAFVYNGDEGSKVAAPIVRRIMEVYFALKQADAGQQP"
     gene            8447..9169
                     /gene="minC"
                     /locus_tag="PKL11_05035"
     CDS             8447..9169
                     /gene="minC"
                     /locus_tag="PKL11_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNZ13000.1"
                     /translation="MDSGVNLQIKGVRDGLLIAVGEGEWPAVLSAILDHIDARLAFFK
                     GARLTLEVGNRVLHAAELGTLRDRLSDRGVTLWAILSSSPVTEQTAQVLGLATRIHAP
                     RPAERERERERERERPASFRPLDTNLEGDNAILVQRTLRSGFRVASHAHIIVLGDVNP
                     GAEVSAGGNIIVWGRLRGNVHAGMDGNENAVVCALELMPTQLRIADMVISLPGRRGKI
                     QPEMLSLRNGQVVIEPWNPKEK"
     gene            9180..9983
                     /gene="minD"
                     /locus_tag="PKL11_05040"
     CDS             9180..9983
                     /gene="minD"
                     /locus_tag="PKL11_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNZ13001.1"
                     /translation="MAAHVVTISSGKGGVGKTTTTANIAVALAMGGQKVACIDADIGL
                     RNLDLILGLENRIVYDLVDVVEGRCRIRQAMIRDKRLPDLYLIPSAQTRDKSAVSPSD
                     MVRLCDELRQEVDWILIDSPAGIERGFRNAMAPADLVIVVTNPEVSAVRDADRVIGLV
                     EAEEKGPARLVLNRLNPVMVKRGDMLAPDDVLELLAVELIGIVPEDDTVVVSTNRGQP
                     VAMDAKSRAGQAFQNIARRLKGEQVPFMDLDEKKGVLGWLSRIARAGGD"
     gene            9985..10248
                     /gene="minE"
                     /locus_tag="PKL11_05045"
     CDS             9985..10248
                     /gene="minE"
                     /locus_tag="PKL11_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNZ13002.1"
                     /translation="MSDWLDRLLGRQPKSASSAKERLKLVLINDRTDLTSSELEALKN
                     ELLEVIGRYIDIDPAAIRIEMMQEGREQRLLADIPLRASRRHR"
     gene            10266..11366
                     /locus_tag="PKL11_05050"
     CDS             10266..11366
                     /locus_tag="PKL11_05050"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNZ13003.1"
                     /translation="MVRRQIWRYFDYWLFGAVIFLCIFGVIMVRSAVAGNAVLAGLPN
                     RQIIWVGISLVVLLGTTLIDYHYWQSLARTMYIVTVLALVGIFIVGEAVYGSARWFDT
                     GVIFIQPSEIAKIVLIMVLANYYAATQDDERNLLWIGKSFLVTLGIVVWILLQPNLST
                     SIVMFVLWFALLFVAGLPIKFLVSFGFGGLALAAAAFPFLENYQQQRILDFVMKDENA
                     RHGNTYNVEQALITIGNGGWFGLGYGHGTQVQLHFLKVRHTDFIFSAIAEEFGFVGTV
                     LLLALLVFVIMRCLRVARRAADPFGAYLAYGVAVLLLFQTTVNVGVNLNLLPVTGLTL
                     PFVSYGGSSLLSLMLGMGLVESVAAHSKPMEF"
     gene            11376..12863
                     /gene="gltX"
                     /locus_tag="PKL11_05055"
     CDS             11376..12863
                     /gene="gltX"
                     /locus_tag="PKL11_05055"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNZ13004.1"
                     /translation="MANTSKPVRVRFAPSPTGHMHLGSARTALYDYLLARQTGGQFIL
                     RIEDTDRKRFVEGAEQELMDGLRWLGIQWDEGPDIGGPCGPYRQSERKAIYQQYAEQL
                     IDSGHAYYCFCSPQRLDAVRKDQQSRKENPRYDGLCRNLPLAEARARIAAGESYVIRF
                     KMPQEGTTVVRDLLRGDITLENRMLDDYVIVKSDGLALYHLAAMVDDHLMGITHVIRG
                     SEWLPTHPLHVNLIRAFGWEEPVWVHLSIFLKPSGKGKMSKRDTAELANDGYSIFVKD
                     LLALGYLPEAIVNWVALMGWSYDDHTEFFTLADLVEKFSLEKLNPAPAAINFTKLDYF
                     NGLHIRSLSIPELAARLRYYFDAANLPVSDADLLRITPLIRERMTTLDEGPELSGYFF
                     RPYAIPTAADLTAKGLTPAQSADVARRVLTILNDLPDVSHAVAEPPLRTLVEELGLSA
                     GQVFGIMRVAVTGQRVSLPLFESMEIIGRAEILNRLQQAIQILEA"
     gene            12889..13170
                     /locus_tag="PKL11_05060"
     CDS             12889..13170
                     /locus_tag="PKL11_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNZ13005.1"
                     /translation="MPEKTYTEEFRVTGEKLVAEVKKIVHEGNVRRIIVKKEDGDTLM
                     EFPLTIGVVGVALLPVWAALGAVAALAANLTIVVERVDQTPPPGSPQPQ"
     gene            13200..14258
                     /locus_tag="PKL11_05065"
     CDS             13200..14258
                     /locus_tag="PKL11_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ13006.1"
                     /translation="MKSISLENHTEENRPEPSKISRRQFLKQLGLVAGAVGLAACTRP
                     PMPSSTPTLTPTGSPTSTPTAAPSATPTHTPTAAPSPTATLAPLEAQIRQQGVATRIP
                     VLEYHSLNSDVNADGLLLMNPVWFEEQMHWLKDNQFHAVTEQELIDFLTGSIQLPKRS
                     VVLTFDVGSKVSKQMPEFVRIFEQTGMHGLFFIWLARMDAGLWDGCKSNADGCWSDYH
                     ILIESGYGEIGSHSMSHADMAVADYDAVVWELRESKARIEQHLSIEVRSFSWPFEAIP
                     TKARQMFTETGYTLGFGGFSRYITELQAYPYPDFSLPVYDIPRLLPYYNNQSYPYLSG
                     RTMGQETYADLLFRNITP"
     gene            complement(14304..>15163)
                     /locus_tag="PKL11_05070"
     CDS             complement(14304..>15163)
                     /locus_tag="PKL11_05070"
                     /inference="COORDINATES: protein motif:HMM:NF023367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HNZ13007.1"
                     /translation="MALLWNTLATRDIGLLAQALSSRFKLPRGTAWVNYVRCHDDIGW
                     TFADDDAARLNINGHDHRQFLNEFYRGRFPGSFARGLPFQENPNTGDCRISGTCASLA
                     GLEKALNEEGPGEVDLAIKRIALIYNIAMTAGGIPLIYLGDEIGTLNDYSYLDHPDHK
                     RDSRWVHRPQADWVKYARRHDSGSIEGRVFQNFKAIIALRKATPALAGGELDVIHTEN
                     PHVLCYSRSMGEQRVVIFANFAEAEQTIAPKVIQQNYLAQKQFLYGEGYMRDDGGLML
                     PPYSFSVLG"
BASE COUNT         3042 a         4481 c         3868 g         3572 t
ORIGIN      
        1 cagcgtcttt tcaccaaata gaacctcacg cagccccttc tctgaaaccg tcagcgtaaa
       61 gcttcccagc ggtgaatcag ccagcagtgc tgaaaataat gggacaaaag gtgcattttt
      121 catctgtttg ttagctttct gtaaggtgag cgtagggtaa gcgtagggta cacgtcaagc
      181 agggtgaaga ggcctctggt attatagact tcgaagggcg acttctcttc ttctcctcct
      241 tgatagtgtg ccggggtcgg cgtccccggc acactctgat tctaacacgc tttggggaaa
      301 tttcagtctg cggcagcgaa tcacaggttg gcaggctaaa attgtgatac actttgtgac
      361 aacatcccgt accggcatca gactagcggg gttaaatgta atataaccca atcccaacaa
      421 gaggtgattt aatggaatta acgatggaaa aagcccgaac acgcgctcgc cgtcgccgca
      481 tccccaccac tcgacgcgaa cgctgggcct ggtattttta tgatttcggc aattcagcct
      541 atgccgccgt agtcctttta gccgtctatt cagcctattt caaaggccag gttgttggcg
      601 gttctgaagg cacccggctc tggggcgtct cagtgggcat tgctatgctg gtggttgccc
      661 tgctctcgcc catcctgggc accttggccg attacactgc ctcaaagaaa aaatacctct
      721 tcatcttctc aagcatggca attgttttta ccgccgcgct tttctttgtt cagcccggca
      781 atatctttat gggcatgttg ttcttcatcc tggccgaaat cggttaccgc agcggacagg
      841 tcttttataa cgccctgtta ggcgatattg cctctccaaa cgaaatgagc cgcgtctccg
      901 gcaacggctg ggccattggc tcggcgggag gcatcgtctg cctgctggtg gtgctgcccc
      961 tcatcgttct ggttggcggc acatttgtgg ttcgcatttc cttcctgatt accgccgctt
     1021 tctacctggc ctcttcaatc ccgatcttca tctggctgcg tgagcgggct gaacctcagc
     1081 ccctgccgca gaaaaccagt gtgctggcgc tggcattccg ccgcctggcc gataccttca
     1141 aggccattcg caattacaaa gaactggtca aatttatcgt cgccttcctg gtcttcaaca
     1201 acggcatcat gatgaccctc gactttgccg ccatcatcgg tgccaccctc tttggcatga
     1261 cgcagaccca gttaattatc tttgtcatca tcgtccagat taccagcgtg ctgggcgcct
     1321 tcctctttgg caaactggca ggccgttgga gcagcaaata ctcgttgatc atctctctgg
     1381 ttttgatgat cgtggctgtg tcgctgttgt ttatcgttga ttcgttggtg atgttcaata
     1441 tcattggcgc actggcgggc tttgccctca cgggtgtcca atctgtcagc cgaaccatgg
     1501 tcggccaaat ggcccctgaa gaacgcagca ccgaatttta tggctttttc tctgtggccg
     1561 gtcagctctc ggccttcatt ggcccggcgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn natcacctat ccccgccggg tggcaatccg gcgggtcttg cgtttcttca
     1741 tccgcctgat atttagcatc atcgcagact ttaagatcga aggacgcgag aatatcccca
     1801 caaatggccc tgtgttggtc attgccaacc atttcagctt cctggatccg gtcgccctga
     1861 ttggtgtttt gccctggacg attgaatttg tcggcggcac caagctgccc aacgctccca
     1921 agtatgtctg gtggctccag cgcctgtatg gcgtcttgca cgtcaaccgc ggcggcgtct
     1981 cgcgcgagac actgctggct tcgcgcaacg tcctcaaaca gggtggcatt ttaggcatct
     2041 tgcccgaagg cggcagttgg gccactgttc tgcgcccccc gcgcccgggc acctcttatc
     2101 tggcaaccac cgttgccgcc cccatccttc cggttggcct cgatggtctg acagatgtct
     2161 tcccgcttaa gctgggccgc cgggcccggg taaccatccg gtttggcaaa ccttttggcc
     2221 ctttctatgt ttcagatcgc ggcgaaacca atcgcacccg cctggatgaa ctgggccacg
     2281 agatgatgcg ccagatttct gtcctgattc caccggaacg ccgcggccat tattcagacg
     2341 atcccgccat tcgggctgcc gcccagggta ccgaaatcta cccctgggcc gaccagaccg
     2401 aattataacg gcgatatatc actgtatttt ttagagagga agaacacaat gaacccaacc
     2461 ccaaaacgct tcccgaataa ctttatctgg ggtagcgcaa cttcatctta ccaaattgag
     2521 ggcgcctgga atgaagacgg ccgcggcctg tccatctggg atacgttttg ccgccaaccc
     2581 ggcacggtct atcacggcgc taatggcgac gtggcagcag accattacca tcgctggcgc
     2641 gaagatgttg cgctgatggc ctctttaggt cttaaagcct atcgtttttc ggtggcctgg
     2701 ccgcgcatcc tgccagaagg caccgggcgc gtcaatcctg ccggcctcga cttttacgac
     2761 cgtctggtag atggcctgct ggagcagggc attgacccct acctgaccct ctaccactgg
     2821 gacctgccgc aaatcctgga agaccggggc ggctggcccg atcgcaagat tgtagatgct
     2881 tttgtagagt attccaccgt ggtcgcccgc cgcctgggtg accgtgtcaa acactggatt
     2941 acccacaacg aaccgatttg caccagctta ttgggttatt tctggggcat ccatgccccc
     3001 ggtattcaga atccggcctc agcgctggca gcagcttacc atgtcattct ctcgcatggc
     3061 gctgcagttc aggctctgcg ttctgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnntggc gctgcggttg ccagagcggc aaaccacaac cacgggcaca tcctgcggca
     3241 cctcggataa ccggctgttc aattctccta acggaatcag cgttgctccc tcaatatgcc
     3301 ccgcattcca ttcctctggt tggcgtacat ctaatataaa agcgccttct gcctgcagtg
     3361 cagccgcctc aaccgccgta atttccaacg gcattgctgt ggcagccgcc tctggtgttg
     3421 gattagctgc ttcaatcgtc gctgtcagcg gcagagtcgc cgtaggtgtg gccgctgccg
     3481 atcttcccgc cagcgttatt ccggtggcca tcaacccgat caccagaacc atcaccccca
     3541 gcaccatcca ggtgcgccgc ggattgacct ttttctgcgt tgttttctga accgccttgc
     3601 gttttgacat gcgttgatct cctctgagca cttggatgct tgtacaaacc aaaagataca
     3661 cagccgtgag aaataattgt accgtaccag tatggtataa tcattccaac tttgcaagtc
     3721 ctgtctcttg acgattttgc ttcttcgcat acgaggagtt gcgtttggct ttcaatccat
     3781 taaattggtt aatggggctg ctatcccttg atattgggat tgatctgggt acagccaaca
     3841 ccctggtaaa tgtgcgcggc aagggtatag tcatcaatga accctcctgg gtcaccattg
     3901 ataaacggac gcgccgcccg ctggctattg gcgcccaggc aaaagaaatg gtcgggcgca
     3961 ccccggcaaa tgtcatcgcc atccgccctc tgcgcgatgg cgtgatctct gagtttgaaa
     4021 ttacccaggc catgctgggt tactttattg gcaaggctca tcagcaaacc atcgttccca
     4081 tgccgcgccc gcgcgttgtg gtgggcatcc cttctggcgc caccgaagtt gaaaagcgcg
     4141 ccgtgtatga tgcttccatg gctgccggcg ctcgcgaaac ctatctgatt gaggagcccc
     4201 gcgccgctgc cctgggcgcc gggttgcccg tctccgaagt ccgcggcagc atggttgtgg
     4261 atatcggcgg cggcaccacc gaggtcgccg tactggcgat gggcggcgtg gtagtttccc
     4321 gctcactccg cgtcgcgggt gatgagctgg atcaggatat catccagtac attcgtaaca
     4381 aatacaacct gttcattggc gagcgcatgg ccgaacaggt caaaatgcaa attggctcag
     4441 cctacccact gcccaacgaa aagaccatga ccgtccgcgg ccgcaacctg gtcaccggcc
     4501 tgcccgagac cctcgaaatc tcttcggtcg aaattcgcga agccgtaggc agttcagtcc
     4561 aggtgattgt cgacaccatc aaggatgccc tggatgagtg tccgccagaa attgtctccg
     4621 atttgattga aacaggcatc tgtctggctg gcggcggtgc catgctgcaa ggcctgatgg
     4681 atcgcctgag tgatgaattg aatctccgcg tctgggtggc cgaggacccg ctcacctgtg
     4741 tggcgcgtgg tgctggcatg atcctggaag acctggatcg ttacgccgac ttcctcgtcg
     4801 ggctggagcg cactggagct gcgccctccg cctgattcgc cgcctggcct ctggcctgat
     4861 ggtgaaatgg aaggcaacag ataatgaaat cccctgtccc tcgcttttgg caaaccgttg
     4921 tggttgtcct gattgttgta gggctgatgc tgttggctgt cagcggctac ctcggacctg
     4981 ttctgcgcgc ggcccaaacg cctctggtcg ctgcccagcg ctggatttcc agccgttatc
     5041 tggcaattta cgagttgatt acttctccta ccgatatggc tgccctgcgc cagcaaaacc
     5101 aggatctgca aaatcaggtt tcccagcttc aaaccgaagt gattgaactt gaacagcgcc
     5161 ttggcgaagc ccaggtgctg tatgccctgc ttgactttgc ccgtgagcag cctcagaaca
     5221 cctacgttgc cgcggccgtg attggccgcg atcccaatcc attcatgcgg tacatcttta
     5281 tcgaccgtgg ctcagattca ggcgtgctgg ctggcatgcc ggtggtcaca gcccagggtt
     5341 tgattggtcg tgtagatgcc gtcacagctc aagcctgtcg tgtcgccttg attaccgacc
     5401 ctgccacggt cattaacgtt accctccaat cgctaaaagc cgatgcccaa ctggttggct
     5461 cactgaccgg cgaactgacc ctcgaaatga tcccctcaga tgttaacctg cagcccggtg
     5521 aactggtctt gacctctggc ctcggcggcg attacccctc tgatgtgctg attgggcagg
     5581 tggtcagcgt ccgcaagctc gaaacggacc tgttccagac ggctacagtc caatcggctg
     5641 tagatttcaa taacctccag gcggtcctgg tcatcaccaa cttccgcccg gtggatattg
     5701 ccccgctgat ccctgaagat taaccatgcc tgttctgatt ggcctgggcg taatcattct
     5761 cttctccctg ctgcaaattg gcgtcatcag tcaactgccg ctgattcagg gccgggctga
     5821 cctgatgctg ttgatcttat cggcctgggc gctgaacgaa cgtgttgaac acgcctggga
     5881 atggaccatc atcgccggcg ttctaatgag tcttatttca gccacgccgt tcttcttgcc
     5941 cctgtttgtc tatctggtgg tcactgccct ggcccgggcg ctgcgccgcc gcatctggca
     6001 aaccccgctg ctgatgttat tgtttattgt cctgattggc accttgcttc aacatgggtt
     6061 aagctttgtc agcctttcaa tcatgggatc gcgcctgccg cttcaagaca gcctcacaat
     6121 ggtgaccctg ccaagtctgc tgctcaattt gctgctggcg ctcccggtct acacaatgat
     6181 tacagatatt gccggatggg tttatccggc ggagttagat gtatgagttc ctctaatggt
     6241 ctggcacaat cgcacatcgc cacctggcgg gttgtggtca tctatgttgc cctggcgctg
     6301 gtcttcagcg tctattctta ccgcctgttc agcctgcaag ttctggaagg cgaaacctac
     6361 cgcacccagg ctgaagaaaa ccgcaccaag atcatcaaca tccaaaccac ccgcggtttg
     6421 atttacgacc gcaacggcta cattctggca agtaatgcac cctcttacaa tgtcgtcatc
     6481 acccccgcag aactgcccgg caaccccgtg ctggtaatga aggcctaccg tgaattgtat
     6541 gattttgatt acaccgaaga agcgggcgcc gtccaggaag tttaccgcaa gctttctgat
     6601 ctgattgatg ttcctgtgat cacgccgaac ttatgggatg aagatggcta tattcaagaa
     6661 attcaagtca agaacttctc cccctgttac agcgatctgg gcatcactga aattgtgatc
     6721 attggcgata ccaatgcccc ttactctccg attgaaatca aatgcaatat tgacgagcgc
     6781 accgccatga tcatccgctc caaggcagcc gaactgcccg gtgtcagcat tgccatcacc
     6841 cctgtgcgtg aatatcctac cggctggctg acatcagaaa tcattggctt cctggggccc
     6901 atccccgcgg ccgaagaaga ctactacgag tccctgggct tggtccccaa ccgcgacaag
     6961 gttggctacg ccggcgttga agccactctg caggatattc tcagtggcac caatggacag
     7021 cgggttgtcg aggtggacaa tgccggccaa atcctgcgcg acattgaagc ccccattgat
     7081 ccaatcccgg gcaataatgt ccgcctgacg attgataccc gcttgcagac cgccgctaaa
     7141 gaaattctgt tggatgaaat ggaaggctgg aacaactggt ttggctacga gcgttataac
     7201 aatggtgttg tcattgccat gaatccccag accggcgaaa ttttggccct ggtctcgtat
     7261 cccagctatg aaaataaccg catggcccgg gagatccccg cctattacta caatcaacta
     7321 atctcagatc ctcgccgccc cctcttcaat catgccatct cagcggagca tcccccgggc
     7381 tccgttttca aaatggccgc ggccattgga attttgaatg aaggcgtggt cacacccgaa
     7441 caagaaatct ttgatgccgg tgtaattgag attgaagaac gcttcacccc caacgatccc
     7501 aaccccggca cgcgtgaata tgtttgctat acctataaga ccaccggcgc cggtcatggg
     7561 gacgtagatt atctgcatgg cattgcgctt tcctgtgatg tctatttcta caaagtcggc
     7621 ggcggctacg aagatgaagt ccccgaaggc ctgggtatct ggcggctggg tgaatatgct
     7681 cgcgccctgg gctatggcag ccagaccggc attgaactcc ccggtgaggg cgatggtttt
     7741 atgcccgacc caacctggaa acgtatctac aaattcgaaa actggtccac cggcgatacc
     7801 tacatctcca ctatcggtca gggttatgta acctcaaccc cgctgcaggt cttggtctca
     7861 gttgccacat tggctaacga tggcaaaatg atgaagccca ccctgattcg ggaagttctc
     7921 gatgctgaag gcaatgtggt cacccccttt accccggtgg aagtctggga cattaccaaa
     7981 gatccactca ttcatgtcta cgatgaaaat tacctccgga ccgatgagct caaaaccgta
     8041 gaaccctggg tgattgatat ggcgcagcag ggtatgcgca tggtggtgac tgaagggact
     8101 gccagcgcct attttgaagg ctttgatatc ccctccggcg gtaaaactgg cacagccgaa
     8161 tactgtgatg atatcgccag tgcgcgggat atttgcaagc ctggcgcctg gcctgcccat
     8221 gcctggtatg tgggttatgc cccctttgat aaccccgaaa tcgccatagt cgcctttgtt
     8281 tataacggcg acgaaggctc taaagtcgcc gcgcccatcg tccgccggat catggaagtc
     8341 tattttgccc tcaaacaggc tgatgccgga cagcagccct gatgttattt atcctcactt
     8401 cggggtataa ttccaccatt aatcaacccg ctggattatg atattaatgg actcaggcgt
     8461 taaccttcaa atcaagggcg tacgagacgg gctgctgatc gctgtcggcg agggcgaatg
     8521 gccggcggtt ctatctgcca ttcttgacca tattgatgcc cggctggctt tcttcaaagg
     8581 cgcccgcctg acgctggaag ttggcaaccg ggttctgcat gcggccgaat tgggcacctt
     8641 gcgagaccgc ctctcagacc ggggcgtcac gctctgggcc atcctcagca gttctcctgt
     8701 caccgaacag actgcccagg tgctaggcct ggcaacccgc atccacgcgc cgcgccccgc
     8761 cgagcgcgaa agagaacgtg agcgcgaacg ggaacgcccg gcttcctttc gcccgttgga
     8821 taccaacctg gaaggggata acgccatcct ggtccagcgc accctgcgct ctggtttccg
     8881 cgtggcctca cacgcccata tcattgtcct gggcgacgtg aaccccggcg ccgaagtcag
     8941 cgctggcggc aatatcatcg tctgggggcg gctgcgcggc aacgttcatg ccggcatgga
     9001 tggcaatgaa aacgctgtgg tttgcgccct ggaactcatg cccacccagt tgcgcattgc
     9061 cgacatggtc atcagcttgc ccggtcgccg tggcaaaatt caacccgaaa tgctcagcct
     9121 gcgcaatggg caggttgtga tcgaaccctg gaatcccaaa gagaaataga gggaggcaaa
     9181 tggctgcaca cgttgtaacc atctcatctg gcaaaggcgg tgtgggtaaa accaccacca
     9241 ccgccaatat tgcagttgct ctggccatgg ggggccagaa ggtcgcctgc attgatgccg
     9301 atatcggttt acgtaacctg gatctgatcc tcggccttga aaatcggatt gtttacgatc
     9361 tggtggacgt ggttgagggg cgctgccgca tccgtcaggc catgatccgc gataagcgcc
     9421 tgcccgattt gtatctcatc ccatctgccc agacgcgcga taaaagcgcc gtctcaccca
     9481 gtgatatggt tcgcctgtgt gatgaactgc gtcaggaagt cgattggatt ttgatcgatt
     9541 ccccggctgg cattgagcgc ggttttcgca atgccatggc ccccgctgat ttggttattg
     9601 ttgtgaccaa tcccgaagtc tccgctgtgc gcgatgctga ccgggttatc ggcctggtgg
     9661 aagctgaaga aaaaggcccc gcccggctgg tactcaaccg cctcaacccg gtcatggtca
     9721 agcgcggcga catgctcgcc cccgatgatg tgcttgaact cctggccgta gaactgattg
     9781 gcatcgtccc cgaggatgac actgtcgttg tcagtaccaa ccgcggccag cccgtggcca
     9841 tggatgctaa atcacgcgcc gggcaggctt ttcagaatat tgctcgccga ttaaaaggcg
     9901 agcaggtgcc tttcatggat ctggacgaga agaagggcgt cctgggttgg ctttctcgga
     9961 ttgcccgggc aggaggcgac taatatgtca gactggttgg atcgcttact gggtcgtcag
    10021 cccaagagtg ccagttcggc caaagaacgc ctcaaattgg tgttgattaa tgaccgcact
    10081 gacctgacct ccagcgaact ggaggcgctt aaaaacgaac tgctggaggt tattggtcgc
    10141 tatatcgata ttgaccccgc agctattcgc attgaaatga tgcaggaagg gcgcgagcag
    10201 cgtttgctgg cagatattcc gctgcgggcc agccgccgcc accgctgatc tttacaggtt
    10261 attttatggt tcgcaggcaa atctggcgat actttgatta ctggctgttt ggggcagtga
    10321 tcttcctctg catcttcggc gtcatcatgg ttcgttcagc cgttgctggt aatgctgtgc
    10381 tggctggctt gcccaaccgt cagattatct gggtgggcat ctcgctggtt gtcctgttgg
    10441 gcaccaccct cattgactat cattactggc agtcacttgc ccgtaccatg tatatcgtca
    10501 ctgtgctggc cctggtgggc atcttcatcg tcggcgaagc agtctatggc tctgcccgct
    10561 ggtttgatac cggcgtcatc ttcattcagc cctccgaaat tgctaaaatc gtcctcatca
    10621 tggtgttggc aaactattat gccgccacgc aggatgacga acgcaatctc ctctggattg
    10681 gtaaaagctt tcttgtcacg cttggcatcg ttgtctggat tctcctgcag cctaacctga
    10741 gcacctccat tgtgatgttt gtgctctggt tcgccttgct ctttgtagct ggcctgccca
    10801 tcaaatttct ggtgtccttt ggctttggcg ggttagcact ggcagccgca gcctttccct
    10861 tcctggaaaa ctaccagcag cagcgcatcc ttgactttgt catgaaagat gagaatgccc
    10921 gccatggcaa cacctataac gtggagcagg ctctcattac gattggcaac ggcggctggt
    10981 tcggccttgg ctatggacac ggcacccagg tgcagctgca cttcctcaag gtgcgccaca
    11041 ccgactttat cttctcagcc attgctgaag aatttggctt tgtgggcact gtacttttac
    11101 tggccctgct tgttttcgtg atcatgcgct gtctgagggt ggcccggcga gccgctgacc
    11161 cattcggtgc ctatcttgcc tacggggttg cggttttgct gttgttccaa accactgtta
    11221 acgtgggcgt caatcttaat ctattacccg tcaccggcct gacactgcct ttcgtcagct
    11281 atggcggcag ctcgctgctc tcactcatgc tgggcatggg cctggttgaa agtgtggcag
    11341 cccacagtaa accaatggaa ttttaggagt ctggcatggc aaacacatcg aaacccgttc
    11401 gcgttcgttt tgcgccctcg cccaccgggc acatgcacct gggcagcgcc cgtaccgctc
    11461 tctatgatta cctgctggcc cgtcagaccg gtggacagtt catcctgcgc atcgaagata
    11521 ccgaccgcaa acgttttgtc gaaggcgccg aacaggagtt gatggacggg ttgcgctggc
    11581 tcggcatcca gtgggacgaa ggccccgata tcggcggtcc ctgtggtccc taccgccagt
    11641 cagaacgcaa ggccatttat caacaatatg ccgagcagtt gatcgattct ggtcatgcct
    11701 actactgctt ctgctccccc cagcggctgg acgctgttcg caaagatcag cagtcgcgca
    11761 aagagaaccc ccgctatgat ggcctctgcc gcaatctgcc tttggcagaa gcccgtgccc
    11821 gcattgccgc cggtgaatct tatgtcatcc gcttcaaaat gcctcaggaa ggcacaaccg
    11881 tggtgcgcga tttactgcgc ggcgatatta cgcttgaaaa ccgcatgttg gatgattatg
    11941 tcatcgtcaa atcggatggc ttggcgctgt atcatctggc cgccatggtg gatgatcatt
    12001 tgatgggtat cacccacgtc atccgcggct ccgagtggct gcccacccac cccctgcatg
    12061 tcaacctcat ccgggccttt ggctgggaag aacccgtctg ggttcacctt tcgattttcc
    12121 tcaaacccag cggcaagggc aaaatgagca agcgggatac tgccgaactg gcaaatgatg
    12181 gctattccat cttcgtcaaa gacttgctgg ctctgggcta cttgccagaa gccattgtca
    12241 actgggtggc cctgatgggc tggagctatg acgatcacac cgagttcttc acccttgccg
    12301 acctggttga aaaattcagc cttgaaaaac tcaatcctgc cccggctgcc atcaacttca
    12361 ccaaactgga ttacttcaac ggtctgcaca ttcgctctct ctcgatcccg gaattggcgg
    12421 cccggctgcg ctactatttt gacgccgcta acctgcccgt atctgatgct gacctgctgc
    12481 ggatcacccc cttgatccgg gaacgcatga ccaccctcga cgaaggcccc gaactctctg
    12541 ggtacttctt ccgcccttac gcaatcccca ccgccgccga tctgaccgcc aagggtctga
    12601 caccagctca atctgccgat gtggcccgcc gtgtcctgac catcctcaac gacctgccgg
    12661 atgtcagtca tgcggttgcc gagccgccgc tgcgcaccct ggtggaggag ttgggtctct
    12721 cagctggtca ggtattcggc ataatgcgcg tagccgttac gggccagcgc gtcagcctgc
    12781 ccctgtttga gagcatggaa atcattggcc gcgctgaaat tctcaaccgg ctccaacagg
    12841 ccattcaaat tctcgaggct taggcccccc cttaaaagga gcaataaaat gcctgaaaaa
    12901 acctacaccg aagaatttcg cgtcacaggc gaaaaactgg tcgctgaagt caaaaaaatt
    12961 gtacacgagg gcaatgtccg ccgcattatc gtcaaaaagg aagacggcga caccctgatg
    13021 gaattccccc tcaccattgg cgtcgtcggc gttgcgctcc tgcccgtctg ggctgctctg
    13081 ggcgcagtgg ctgctcttgc agctaacctc accatcgttg tcgaacgggt cgatcagacc
    13141 ccgccgcccg gctctcccca gcctcagtag cgttttgcct tgattgactc ggagacatcg
    13201 tgaaatcaat ctcattagaa aatcacaccg aagaaaaccg ccccgagcct tcaaaaattt
    13261 cccgccgcca gtttcttaaa cagcttggcc ttgtggctgg agcggttggt ctggctgcct
    13321 gcacgcgccc gccaatgcct tcctccacac ccaccctcac accaaccgga agcccaacgt
    13381 ccacaccaac ggctgcgccc agcgccaccc ctacccatac cccaaccgcc gcgcccagcc
    13441 ccaccgccac ccttgcccca cttgaagctc aaatccgcca gcaaggagtc gccacccgca
    13501 ttcccgtcct ggaatatcac agtctgaatt ccgatgttaa tgccgatggc ctgcttctga
    13561 tgaatccggt ctggtttgaa gagcaaatgc actggctcaa agataatcag ttccatgccg
    13621 tcaccgagca ggaactgatc gacttcctca ccggcagcat ccaacttccc aagcggtccg
    13681 tcgtgctgac ctttgatgtc ggctccaaag tctccaagca aatgcccgag tttgtccgca
    13741 tcttcgagca gaccggcatg catggcctct tcttcatctg gttggcgcgc atggatgccg
    13801 gactttggga tggctgcaaa tccaacgccg atggctgctg gtccgattac cacatcctga
    13861 ttgaaagcgg ctatggcgag attggctctc attccatgtc tcacgctgat atggctgtgg
    13921 ccgattacga tgctgttgtc tgggaactgc gtgaatctaa agcccgcatt gagcagcacc
    13981 tctccattga ggtgcgctcc ttctcctggc cttttgaagc cattcccacc aaagcccgcc
    14041 agatgttcac cgaaaccggc tataccctcg gatttggcgg tttctcccgc tacatcaccg
    14101 aactgcaggc ctacccctac ccggattttt ccctgcccgt ctacgatatc ccccgtctgc
    14161 tgccctatta caacaatcaa tcctacccct acctctccgg tcgcaccatg gggcaggaga
    14221 cctatgccga tctgttgttt cgcaacatca ccccctgagt gggggtgatg ttgctttttc
    14281 gcctggcttt gtgctgaaag gccttatccc agcacactaa atgagtaggg cggcagcatc
    14341 aggcctccat cgtcccgcat gtatccctcg ccatacaaaa actgcttctg cgccagataa
    14401 ttctgttgaa tgaccttggg cgcaatagtt tgttccgctt cagcaaagtt ggcaaaaatg
    14461 accacccgct gctcgcccat tgaccggctg tagcacagca cgtgcgggtt ctctgtatgg
    14521 atgacatcca attcgccccc cgccagcgca ggggtggcct tccgcagtgc aataatcgcc
    14581 ttgaagtttt gaaacacccg cccctcaata ctgccggagt catgccgcct ggcatacttc
    14641 acccagtcag cctgtgggcg gtgcacccag cggctgtccc gcttatgatc cggatgatcc
    14701 agatagctgt aatcgtttaa tgtcccaatt tcatccccca ggtaaatcaa cgggatcccc
    14761 cccgccgtca tggcaatatt atagatcagt gcaatccgct taatcgccag atctacctca
    14821 cctggcccct cttcgttgag cgccttctcc agccctgcca gcgacgcaca tgtgcccgat
    14881 atccggcagt caccggtatt ggggttttcc tgaaacggca gcccgcgcgc aaaactaccc
    14941 ggaaaacgtc cccggtaaaa ctcgttcaaa aattgccggt ggtcatgccc gttgatattc
    15001 aatcgcgccg cgtcatcgtc agcaaacgtc cagccaatat catcatggca gcgcacataa
    15061 ttgacccagg ctgtgccgcg cggtagttta aatcggctcg aaagcgcctg cgccagcaag
    15121 ccgatatcgc gggttgccag ggtattccac agcagcgcca tca
//
LOCUS       DAOPVS010000039        14741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4154_length_14741_cov_48.298175, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000039 DAOPVS010000000
VERSION     DAOPVS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4154_length_14741_cov_48.298175"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PKL11_05075"
     CDS             complement(<1..703)
                     /locus_tag="PKL11_05075"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNZ13008.1"
                     /translation="MRAVDIIIKKRDRAELTGDEIDFFIDGLTHGSIPDYQASAWAMA
                     VLLNGMTERETTDLTLAMAHSGETLDLTGVVPIAVDKHSTGGVGDKTTLVVEPIVASC
                     GLPVGKMSGRGLGFSGGTLDKMESIPGYRTNLTREEFLHQLKQIGLVLTGQSGDLAPA
                     DGKLYALRDVTGTVPSIPLIASSIMSKKIAAGAQAIVLDVKVGVGAFMETLEDARRLA
                     ALMVAIGRLSGRKTVA"
     gene            complement(810..1580)
                     /gene="jag"
                     /locus_tag="PKL11_05080"
     CDS             complement(810..1580)
                     /gene="jag"
                     /locus_tag="PKL11_05080"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNZ13009.1"
                     /translation="MTTEKTTLEVIASTVEEATAKGLAQLGLPASSVDIEVLDAGSRG
                     LFGLGGRQARIRLVLKDITEPAPAASRPEPVVVAATPVETTPKPNHAAEPTETMDPAS
                     AESARQVVSELLEKMKVQAQVTAHWTPPQDEKDRAMVVVEVQGQDLSILIGRQSETLN
                     ALQYITSLIVSKEVGHWVPLLIDIQGYRSRRERQLRQLARRMAEQVIQYGRRQVLEPM
                     PASERRIIHLELRDHPQVTTESEGEEPTRKVVILPKKQ"
     gene            complement(1596..2522)
                     /locus_tag="PKL11_05085"
     CDS             complement(1596..2522)
                     /locus_tag="PKL11_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNZ13010.1"
                     /translation="MWDALIVTPFINALLFIYNLVGQNFGIAIILFTILIRLITHPLM
                     ASQIKGSKSMQALQSDPEYKEMMEKYKGDREKLAAEQMRIYKEKNIKPFASCLPTLIQ
                     FPIIIGLYQAIIAALASTPVELINLTRHIYPALLKVEALIPLNARFLWMDLGQPERLN
                     LPFLSFGIPVLAIVVVVTTFLQSKLITPPSSNPNDQGAQMAKMMNWYMPIFMGYLAWT
                     LASGLSLYFVISNLIGIGQYALLGSLNWSNLLPKSKRPAPAAAVAGPTKKSDSVVQLA
                     TSSRPEPQKKADDSSTNRGNKQIRPPRKKNRS"
     gene            complement(2534..3610)
                     /locus_tag="PKL11_05090"
     CDS             complement(2534..3610)
                     /locus_tag="PKL11_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HNZ13011.1"
                     /translation="MKQSRYLILVLFVLAAALLSACTGAMTASSWPGITPQDDTIYVA
                     NSSFVYALRPADGGEIWRFPEKAEGQKMFYAAPVLADGQLIVGDYQHTLYSLNPATGR
                     ENWRFEDATNRYIASPLVVDGLILAPNADHVLYALDMDGKLVWSYETGQPLWASPVSD
                     GSHIYLAGMDHNIYALDAQTGELVWQKDLGAAMLYTPTLSEDGHLYVSTLKNTIVVLD
                     ANGTVAWEWQGDAAVWAQPALRDGRLYFGDQLGVLYCVDAEKGSVIWTIDAGGEDDIA
                     AFGTPLLLENVAVFSMENGDVIGVSYEGAKQWTRSITGTLYAGPVLAGDRFLVGVNKG
                     ESLIVTFDLNGNESWSFKLDSKKK"
     assembly_gap    3627..3726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3784..4065)
                     /gene="yidD"
                     /locus_tag="PKL11_05095"
     CDS             complement(3784..4065)
                     /gene="yidD"
                     /locus_tag="PKL11_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNZ13012.1"
                     /translation="MNLPKDYPNEPRLRDLPFRWVNLPRIPLLALIRAYQAVISPAMP
                     VDTCRFYPSCSHYGYQAIYKYGALKGGWMAFTRVLRCNPWNPGGYDPVP"
     gene            complement(4494..4667)
                     /gene="rpmH"
                     /locus_tag="PKL11_05100"
     CDS             complement(4494..4667)
                     /gene="rpmH"
                     /locus_tag="PKL11_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNZ13013.1"
                     /translation="MSTKRTYQPKIRRRVRVHGFRSRMATADGREVLKRRRAKGRHKL
                     VVSMNNHVKQSRW"
     gene            complement(4734..5420)
                     /locus_tag="PKL11_05105"
     CDS             complement(4734..5420)
                     /locus_tag="PKL11_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNZ13014.1"
                     /translation="MYVVIAGGGRTGSHLARLLLSQDHEVHLIEDRKDVLTMNHRELP
                     TESIHEGNPMSPAVLEQAGIRRADVLAACTTHDELNLVLCYVARARYGIARTIARVNN
                     PRDAWLFDQKFHVDVALNQAEIMANMIQEEMSLGDMMTLLRLRRGNYALVEEKIPENA
                     PAVGQAIKDMHLPEKCVIAAIIRSGEVIAPRGQTVFQVGDEVLVFTDADSIHDLAELF
                     SDSQDNPSIV"
     gene            complement(5423..6079)
                     /locus_tag="PKL11_05110"
     CDS             complement(5423..6079)
                     /locus_tag="PKL11_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNZ13015.1"
                     /translation="MKILVVGSGRMGAELAYRLYRQGHEVCVVDGFHESFQYLPADFQ
                     GRVYEGEVINQEVLHRAGIEHCDALAAVTNSDALNLVVATIARDHYRVPQVLARNYDP
                     IFAELYDTFGIQFISATTWGAQRLEEMIYHGEVRSIFSAGNGEVDIYELKVPERWCAR
                     PLQELVPAELVVASLTRNGEAMIPAPDTMLETGDLLHVAATLSGLEALSARLEGKKEA
                     "
     gene            complement(6100..>6446)
                     /locus_tag="PKL11_05115"
     CDS             complement(6100..>6446)
                     /locus_tag="PKL11_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ13016.1"
                     /translation="RYARTLSDDITAVHVSIDPIESEKIKDKWETWGNGIRLVILESP
                     YRLLIEPMLEYIEELQEHRQPNELITIVVPQFVSKQIFASVLHTHTADTLRNVLLNRE
                     GIVITEVPYQVE"
     assembly_gap    6447..6546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6547..8201)
                     /locus_tag="PKL11_05120"
     CDS             complement(<6547..8201)
                     /locus_tag="PKL11_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNZ13017.1"
                     /translation="MNINNEHGTTVLRRTADYKPPRSIRTWLVGRPLQTADAPHQTIG
                     KLVGLAVFSSDAMSSVAYAPQEMLIILAAAGMGALGISIPLSLAICGLLVILTLSYEQ
                     TIHAYPGGGGAYIVARDNLGEIPALTAGAALLTDYILTVSVSISSGVAQLVSAYPALH
                     DYRVWISVAMVLLVMLINLRGVKESGFAFAIPTYFFLVMMFVTVGVGFVRYLAGTLGM
                     VSAPPMDLMMEHEVLQPITIFLILKAFASGTTALTGVEAISNGITAFKTPRSKNAGTT
                     LIMMAVILGTLMFGITFLGHQMGVLPSETETVISQMARTVFDGRGLVYLLALTSTTVI
                     LMMAANTAFADFPRLGALMATDGFLPRQLAYRGSRLVYSYGIAALAIIASLLIIVFQA
                     SVTRLIPLYAIGVFLSFTLSQSGMAHRWFKSGRLRLGQKLTERGSTLEHDRSWLFKMI
                     VNGFGALCTLVVMIVFAVTKFSDGAWVVIILIPGLAAIFLTIHRHYARLAQKLSLEHY
                     GRTPHVGRHRVVLALGGVHRGTLEALRYARTLSDDITAVHVSI"
     gene            complement(8467..>8996)
                     /locus_tag="PKL11_05125"
     CDS             complement(8467..>8996)
                     /locus_tag="PKL11_05125"
                     /inference="COORDINATES: protein motif:HMM:NF027968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ13018.1"
                     /translation="PLSPGAYELEMRLMGASPTEALQHAAFNGRDKCRTPLQWSNAPH
                     GGFCPSQVKPWLPVNANYAAGVNVADQQSDSNSLWQTYRTLLHIRQQNPALIDGDYQP
                     LLEKDTEVLAFIRSTASQRCLVIINTASEERSIRLKQLMQTREVIYTNTSRPLPDWTD
                     LTLAPFEILLLALPA"
     assembly_gap    8997..9096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9097..10322)
                     /locus_tag="PKL11_05130"
     CDS             complement(<9097..10322)
                     /locus_tag="PKL11_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ13019.1"
                     /translation="MTTPKWWQRAVFYQIYPRSFADGNGDGIGDFTGMISKLDYLKDL
                     GVDAVWLSPHYPSPMADCGYDVADYTAVAPEYGTLDDFRRFLNGLHERGMYLILDLVL
                     NHTSDQHPWFIESRSSKTNPKRDWYVWRPGKNGQPPNNWCATFGGPAWEYDPQTDEYY
                     YHFFFKEQPDLNWHNPEVRQAMWSAVRFWLDMGVDGFRLDAIGTIFEDPAMPDHQSKL
                     TLDDLYRYNRLAQTPEEQANSGKLWEGIFAAQHDQPGMHELVQELRAVINEYPDRVLV
                     GETDDLTYYGSGEDELHLVFNFPLMRTRRLNAAWVRDNQQERAAGMPPDAWPCNTLGN
                     HDVDRMISQFGDGVNDAAIARVNLAILLFLRGTPFLYNGEEIGMTNTWVSAVSEFRDP
                     LSPGAYELEMRLMGASP"
     assembly_gap    10627..10726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10727..11595
                     /locus_tag="PKL11_05135"
     CDS             <10727..11595
                     /locus_tag="PKL11_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ13020.1"
                     /translation="IQAGEIFSLLGPNGAGKTTTISMLSTLLAPTSGDAQIGGHSVVH
                     APMAVRQLIGVVPQEIALYDDLNAAENLFFFGRMHGMGGAALKQRVAEVLEQVGLTDR
                     AKSRVKTFSGGMKRRLNIGVGLLHRPQMIFMDEPTVGIDPQSRRNILDAVKELNRQGL
                     TVLYTTHYMEEAQELSDRVGIIDHGELIACGTQKELTQLVGQHETLRLHLNDEINADD
                     LAQKLEALPQILKASAADHQVVLMVTEAEEALPGVIATANALNIKIRSVDIEEPNLEA
                     VFLHLTGRALRD"
     gene            11606..12862
                     /locus_tag="PKL11_05140"
     CDS             11606..12862
                     /locus_tag="PKL11_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13021.1"
                     /translation="MKKIFNIALKDLLINFRDPMGLVLMLVAPLALTLVIAAAFGGVG
                     GGGSAGLADIPVVIVNHDTGDLSPYLVEAFYSEELAELVEPSTVDDDAAARAAVDADE
                     VAAAVIIPDNFSESILPAGLVQGDISSLDSKIVSVVEVYANPGRPVSATVIRSIVDTV
                     LNQFVSGRIGSEITINQLIASGRLSPAEAQQQGMIIGQSLGAAEPTERITLSEETGTA
                     SSGFNWLEYSAPSLAILFMMFTLSAAGRTLLIERDHGTLPRMLVSPTSRAEILGGKLL
                     GNFLTGLLQMTILIVAGGFLFRISWGDPLAVGLLTLAVVAAVTGWGMLPAAFARTPGQ
                     ANAIGTAITLTFGAAAGSFVPRGALPEWLQKAGLVTPNAWGIEGYYNLIKGATLTETI
                     PAILALLVMAIILFGIAVLAFRRQYA"
     gene            12872..14080
                     /locus_tag="PKL11_05145"
     CDS             12872..14080
                     /locus_tag="PKL11_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13022.1"
                     /translation="MRKILALAWNDIQLEFSERSTLVFFLILPLVFTAIIGVALQNTY
                     TDGSEDPRLPLIVVNEDQGTYATELISLLDGSDTVRVVEASAQAAEEMINDDFPAILT
                     IPAGFSQKISTGEQAPLQLYKSPDSNAPVGIEQAVKTAAARLSAALNIAEQSTAEAEK
                     IRPFASETERQTYFEESLAAARAALANPPTRSETTRSENVNTQQIATGFEQSSPGQLV
                     TWVMITLLGGAEVFVNERLNGTLRRLLTTPTTKGIIITGKIAGRLILGIIQMVLLVGF
                     GAVALGVNWGKSPAALAVMLLSFGLAGTALGVMLGAFARTRRQASGLTTLFSMLMAAL
                     GGAWWPLEVTPPAYQAAVKVIPTSWAMTGFTDVILRGQGVSGILLEAGVLLGFAALFF
                     AIGVWKLKFE"
     gene            complement(14143..>14741)
                     /locus_tag="PKL11_05150"
     CDS             complement(14143..>14741)
                     /locus_tag="PKL11_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ13023.1"
                     /translation="LGPDFWQLWQNPRLCRHLHLPLQSGCAATLRRMARKVTPESFSG
                     LVQAARAVAPEIAITTDVIVGFPGETEAEFAESLDFIQEIQFAGGHVFTFSPRPGTPA
                     ERLPGLPTMDVRKQRNARLRAVFAAAAESYHRQFMGQVLPVLWESTDSLGPQGWRLHG
                     LTDNNLRVTALSPQRLWNQITPVRLDALTPDGLSGVIL"
BASE COUNT         3168 a         3649 c         4543 g         2981 t
ORIGIN      
        1 gggccacggt cttgcggccg ctcaggcgcc caatggcaac catcaacgca gccaggcggc
       61 gggcatcttc cagggtttcc atgaaggcgc caacaccaac tttgacatcc aggacaatgg
      121 cctgggcgcc ggcagcgatt ttcttgctca tgatggagga ggcgatcaag ggaatggagg
      181 ggacggtgcc ggtcacatca cgcagggcgt agagcttgcc gtcagcaggg gccaggtcgc
      241 cgctttgacc ggtgagcacc aggccaatct gcttgagttg atgtaaaaat tcttcacggg
      301 tcagattggt gcggtagccg gggatggatt ccattttgtc gagggtgccg ccgctgaagc
      361 ccaggccacg gcccgacatt ttgccaactg gcaggccgca ggaagcaacg atgggttcaa
      421 cgaccagggt ggttttatcg ccgacgccgc cagtggagtg tttatccacg gcaatcggta
      481 ccacgccggt gaggtccagg gtttcgccgg agtgggccat cgccagggtc aggtcggtgg
      541 tttcgcgctc ggtcatgcca ttcaacaaga cagccatcgc ccaggccgag gcctgataat
      601 cggggattga gccgtgggtc agcccatcaa tgaagaaatc aatttcgtcg ccggtcaatt
      661 cggcccgatc tcgttttttg atgatgatat ctactgcacg catcttatct ctccttacac
      721 aatcggcaat taagcagggc ggcccgcaat gggttgcgct tcattgcggg cggccatcag
      781 cgccggaaaa tctggcgatt tagattatct tactgttttt taggcagaat cacaaccttg
      841 cgggtgggct cttcgccttc gctttcggtt gtcacctggg gatgatcgcg cagttccagg
      901 tggatgatgc gtcgctcgct ggcgggcatg ggctcgagca cctggcggcg gccatattga
      961 atgacctgtt cggccatacg gcgggcaagc tggcgaagct ggcgctcgcg gcgggagcgg
     1021 tagccctgga tgtcgatcag cagcggcacc cagtgaccaa cctctttgct gacgatcagg
     1081 ctggtgatgt actgaagagc gttgagggtt tcggactggc ggccaatcag gatgctcagg
     1141 tcctggccct ggacttcaac cacaaccatg gcgcggtctt tctcgtcctg gggcggggtc
     1201 caatgggcag tgacctgcgc ctggactttc attttctcca gcagttcaga gaccacctgg
     1261 cgggctgatt cagccgaggc cgggtccatg gtttcggtgg gttcggcggc gtggttgggt
     1321 ttgggggtgg tttcaacagg tgtagccgcc acaaccacag gttccggccg ggatgcggcc
     1381 ggggcaggtt cggtaatatc tttcaagacc aggcggatgc gggcctggcg gccccccagg
     1441 ccaaagaggc cccggctgcc agcgtccagc acttcaatat caaccgagga ggcgggcagc
     1501 ccgagctgcg ccaagccttt ggcggtggct tcttcaacag tggaagcaat aacttcaagg
     1561 gtcgtttttt cggtagtcat gttcaacctc cctgattatg agcgattctt cttgcggggc
     1621 gggcgaattt gcttgttgcc gcggttggtg gatgaatcat ctgctttttt ctgcggttct
     1681 gggcgagacg atgttgcaag ctgaacaaca gaatcagact ttttggttgg gccagccaca
     1741 gcagccgccg gcgcaggacg tttggatttt ggcaaaagat tggaccagtt gagactgccc
     1801 agcagggcgt actgaccgat accaatcaag tttgagatga caaagtagag cgagaggccg
     1861 gaggccaggg tccaggccag gtagcccatg aagatgggca tgtaccagtt catcattttg
     1921 gccatctggg cgccctgatc gttggggtta gaagatggcg gggtgattaa cttggactga
     1981 aggaaggtgg tcaccaccac cacaatcgcc aggacaggga tgccgaacga gaggaaaggc
     2041 aggttaaggc gttcgggctg gccaaggtcc atccagagga agcgggcgtt caacggaatg
     2101 agcgcttcaa ctttgagcag cgcagggtaa atgtggcggg tcaggttgat gagttcaacc
     2161 ggggtgctgg ccagggcggc gataatggcc tggtagaggc caatgataat ggggaactga
     2221 atgagcgtgg gcaggcacga ggcaaaaggt ttgatgttct tttccttgta gatgcgcatc
     2281 tgctcggcgg ccagtttttc acggtcgcct ttgtatttct ccatcatctc tttatattcc
     2341 gggtcgcttt gcagcgcctg cattgacttg gagcctttga tctggctggc catcaagggg
     2401 tgcgtaatca ggcggatcag gatggtaaag aggataatcg cgataccaaa gttttgacca
     2461 accagattgt aaatgaagag tagagcatta atgaacgggg tgacaattaa ggcgtcccac
     2521 ataacggcat cctttatttc ttcttgctat caagcttgaa tgaccagctt tcgttgccat
     2581 tcaagtcaaa ggtcacaatg aggctttcgc ctttgtttac accaaccagg aagcggtcac
     2641 cagccagcac gggcccggca tacagtgtac cagtgatgct gcgggtccac tgtttggcgc
     2701 cttcgtagct gacgccaatg acatctccgt tttccatgga gaagacggcg acgttttcaa
     2761 gcagcagcgg ggtgccaaaa gcggcaatat cgtcttcgcc gccggcatca atggtccaaa
     2821 tgaccgaacc tttttcggca tctacacagt agaggacacc cagttgatcg ccaaagtaga
     2881 ggcggccatc acgcagggcg ggttgagccc agaccgcggc atcaccctgc cactcccagg
     2941 ctaccgtacc gtttgcgtcc agcaccacga tggtgttttt gagcgtgctg acgtagagat
     3001 gaccatcctc actcaaggtg ggggtgtaca acattgcagc gccgaggtct ttttgccaga
     3061 ccagttcgcc ggtctgcgca tccagggcgt agatgttgtg atccatgcca gcaaggtaga
     3121 tgtggctgcc atcgctgacc ggagacgccc agagcggctg accggtttca taagaccaga
     3181 ccagcttgcc atccatatcc aaagcataga ggacgtgatc ggcattaggc gcgaggatga
     3241 ggccatccac caccaggggg ctggcgatgt agcggttggt ggcgtcttca aaccgccagt
     3301 tttcccggcc ggtggcagga ttcaggctgt agagcgtatg ctggtaatcg ccaacaatca
     3361 attgaccatc ggccaggacg ggagcagcat aaaacatctt ctggccttcg gctttttcgg
     3421 ggaagcgcca gatctcgccg ccatcggctg gacggagagc gtaaacaaaa ctggaattgg
     3481 cgacgtagat ggtgtcatcc tgaggcgtta tgccgggcca ggatgaagcc gtcatggcgc
     3541 cggtgcaggc tgagagcagc gccgcagcca aaacaaacaa gacgagaatc aaatagcgag
     3601 attgtttcat agagtttcca ttcgttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnaaca aacaagacga gaatcaaata gcgagattgt ttcatagagt ttccattcgt
     3781 tgatcagggg acggggtcgt agccgcccgg attccacggg ttgcagcgca gcacacgggt
     3841 gaaggccatc cagccgcctt tgagggcgcc gtatttataa atggcctggt agccgtagtg
     3901 tgaacaactg ggatagaaac ggcaggtatc cactggcatt gcgggggaga tgaccgcctg
     3961 gtaagcccga atgagcgcca gcagcgggat gcgcggcaaa ttgacccacc ggaaaggcag
     4021 gtctcgcagg cgcggttcat tggggtagtc tttaggaagg ttcaccggga ggcggatctt
     4081 gtagcagcaa gccagcgcgg cctaacagtg tttgcagggc agtttgaagc tgatcaaaag
     4141 ttgcgccgtg aatgggttta cgcgccagca ggatcagatc ccaaccaggt gtcagtctgg
     4201 gaagcagccc gtccatacag gctctgatgc ggcgtttggc gcgattgcgc tcgaccgcgt
     4261 tcccggtcac acgaccggct gccaccccga cccgcaccgc tgacgattcc cccggagcaa
     4321 ctattaacac gacaagcggg tgagcgtagg tcttgccaga ccgccgcacc cgctgaaagt
     4381 cggtaaatcg tgtcagacga aaagatcgct tcacccatat ccctgtccat aacggtcagg
     4441 ctgtatgccc agcctgcaga tcaaaccgtt tcatccaagg gcgagggtgt attctaccac
     4501 cgactttgct tgacgtgatt gttcatgctc acgacgagct tgtggcggcc cttggcgcgg
     4561 cggcgcttca aaacctcgcg gccatcggca gtggccatgc gagaccggaa gccgtgcacg
     4621 cgcacgcggc ggcggatctt gggttgatat gtacgcttgg ttgacatctt tatcctttca
     4681 ctttctggtt acgtgataac gagcggcaat tataccgcag cattgtgata cggtcaaacg
     4741 atggacgggt tatcctgcga atccgagaac aattcagcca ggtcatggat actgtcggca
     4801 tcggtgaaga ccaggacctc atcgccaacc tggaaaacag tctgcccgcg cggggcaatg
     4861 acctcacccg agcggatgat ggcggcgatg acgcattttt cggggaggtg catgtctttg
     4921 atcgcctgcc caacggcggg ggcgttttcg gggattttct cttcaacgag ggcatagttg
     4981 ccgcggcgga gccgcaagag ggtcatcatg tcgccgaggg acatttcttc ttgaatcatg
     5041 ttcgccatga tttcggcctg gttgagggcg acgtcaacgt ggaacttctg gtcaaacaac
     5101 caggcatcgc gtgggttgtt gacgcgggca atggtgcggg caatgccgta gcgggctcga
     5161 gccacataac acaataccag gttcagttca tcgtgggttg tgcaggcagc cagcacatcg
     5221 gcgcggcgga tgccagcctg ctccaggact gccgggctca tggggttacc ctcatggatt
     5281 gattcggtcg ggagttcccg atggttcatc gtgagaacat ccttgcgatc ttcaatcaga
     5341 tggacttcgt gatcctggct gagtaacagc cgggctaaat gcgaaccggt tcgaccacca
     5401 ccagcaataa cgacatacat aattacgcct ccttcttgcc ttccaggcgg gcgctcagcg
     5461 cttcgaggcc gctgagtgtg gcagcgacat gcagcaggtc gccagtttca agcatggtat
     5521 cgggtgcggg gatcatggct tcgccattcc gggtgaggga agcgaccaca agttccgcgg
     5581 gcaccagctc ttgcagcggg cgggcgcacc aacgctccgg gacttttaat tcataaatat
     5641 ccacctcgcc attacctgcc gagaagattg agcgcacttc gccgtggtag atcatttctt
     5701 ccaggcgctg ggcgccccag gtggtggcgc tgatgaattg aatgccgaag gtatcataca
     5761 actcggcaaa aatcgggtca taattgcggg ccaaaacctg cggcacgcgg taatgatcac
     5821 gggcgatggt ggcaaccacc aggttgaggg catctgaatt ggtgacggca gcaagcgcat
     5881 cgcagtgttc aatgccggcg cggtgcagca cttcctggtt aatgacttca ccttcataga
     5941 cccggccttg aaaatcggcc ggcaggtact ggaaggattc gtggaaacca tctaccacgc
     6001 aaacttcatg tccctggcga tagaggcgat aagccagctc agcgcccatt cgcccactgc
     6061 caacaacgag tattttcatt ctctttttct ccttgcccat cattcaacct gatagggcac
     6121 ttcggtaatg acgatgcctt cacggttcaa cagcacgttt cgcagagtgt cagcggtgtg
     6181 tgtgtgcaag acactggcga aaatttgttt ggaaacgaac tgcggcacaa caattgtaat
     6241 caattcattg ggctggcggt gctcctgcag ctcttcaata tactccagca tgggttcaat
     6301 cagcaggcga taaggggatt ccagaatcac cagacgaatg ccattgcccc aagtttccca
     6361 tttgtccttg attttttcag attcaatcgg atcaatagag acatgcactg cggtgatgtc
     6421 atctgacagg gtgcgggcgt agcgcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnntcaa tagagacatg cactgcggtg atgtcatctg acagggtgcg ggcgtagcgc
     6601 agcgcctcga gcgtgccgcg gtgaacgccg cccaacgcca ggaccacgcg atggcgaccg
     6661 acatgcgggg tgcgaccata gtgctcaagg gagagctttt gagccaaccg ggcatagtgg
     6721 cggtgaatgg tcaagaaaat ggccgccaga cccggaatta gaatgatcac tacccaggcg
     6781 ccatcggaga atttggtgac agcaaaaacg atcatgacga ccaaggtaca cagggcgccg
     6841 aagccattca caatcatttt gaaaagccag gagcgatcat gctcgagggt ggagccacgc
     6901 tcggtcagtt tttggccaag gcgcaggcgg ccagacttga accagcggtg ggccatgccc
     6961 gactgggata gtgtgaacga gaggaagacg ccaattgcat agagcgggat gagacgagtg
     7021 acgctggcct ggaaaacgat aatcagtagg ctggcgataa tggccagggc ggcaatgccg
     7081 taagagtaaa ccaggcggct gccgcggtag gctaactggc ggggcaagaa gccatcggtg
     7141 gccatcaggg cacccaggcg ggggaagtcg gcgaaggcgg tgttggctgc catcatcaag
     7201 atgacagtgg ttgaggttag cgccagcaaa tagacgaggc cgcgcccgtc aaatacggtg
     7261 cgcgccattt gagaaatgac ggtttcggtt tcagaaggca aaacgcccat ctgatgtccc
     7321 aggaatgtga tgccaaacat cagggtgcca aggattaccg ccatcatgat gagggttgtg
     7381 ccggcatttt tgctgcgagg tgttttgaag gctgtgatgc cgttggaaat ggcctcaacg
     7441 ccggtgaggg ccgtggtgcc gctggcaaaa gctttgagga tcaggaagat tgtgatgggc
     7501 tgtaaaacct catgctccat catcaggtcc atgggggggg cggataccat gcccagagtg
     7561 cccgccaggt agcgcacaaa gccaacaccc acggtcacaa acatcatcac caggaagaag
     7621 taggtgggaa tggcaaaagc aaagcccgat tctttgacgc cgcgcaggtt gatcaacatg
     7681 accagcagca ccatggcaac agaaatccag acgcgatagt cgtgaagggc aggataagcg
     7741 gaaacaagct gggccacgcc ggatgagatg gagacggata cagtcaggat gtaatcggtg
     7801 aggagcgcgg cgccagcggt cagggcggga atttcaccca ggttgtcacg ggcaacgata
     7861 taggcgccgc cgccgccggg gtaggcatga atggtttgct cgtaagacag cgtgagaatg
     7921 accaacagtc cacagattgc cagggagaga gggatagaaa tgcccagggc gcccataccg
     7981 gccgcggcaa ggataatcag catttcctgg ggggcatagg ccaccgaaga catggcatcc
     8041 gaagaaaaaa ctgccagccc caccagcttg ccaatggtct ggtgcggggc atctgcagtt
     8101 tggagcggac ggccaaccag ccaggtacgg atggaacgcg ggggcttgta atccgccgtc
     8161 cgacgcagca cggtagtgcc atgctcatta ttgatgttca tatgtattaa ttacactttc
     8221 tcagacaaac aaattggcgg cgttttcgag tccgagccac caaaggcagg gattatagtc
     8281 ccgacccgtc agcacgtcaa tataaaagcc ccggcgtgcg ggcacgtcca aggctcgtag
     8341 aatgttatgg tttggttaat gtattatacc cgaaatttga gtaaatttat cgggctggtc
     8401 atcaaagcac gggcttttac ggcttgcacc ctgcaagatg caggggtgca agccggtagt
     8461 agaaaatcaa gccggaaggg cgaggagcaa gatttcgaag ggggccaggg taaggtcagt
     8521 ccagtctggc agcgggcggg aggtgttggt gtagataacc tcgcgggtct gcatgagctg
     8581 cttgaggcgg atagagcgtt cttcggaggc agtgttgatg atgaccagac agcgctgcga
     8641 tgccgtagag cggataaagg ccagcacttc ggtatctttt tcgagcaagg gctggtaatc
     8701 accatcgatc agggcggggt tctgctggcg aatatgcagg agtgtgcgat aggtttgcca
     8761 gagcgaattg gaatcggatt gctggtcggc gacatttacc ccggcggcgt agttggcgtt
     8821 gaccggcagc cagggtttga cctgggacgg acaaaaaccg ccgtggggag cattggacca
     8881 ctgcagcggc gtgcggcatt tatcacgccc gttgaaggcg gcgtgctgca aagcctcggt
     8941 tggagaagca cccatcaggc gcatttcaag ctcataggcc ccagggctga gcggatnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggag aagcacccat caggcgcatt
     9121 tcaagctcat aggccccagg gctgagcgga tcacgaaatt cggagacagc ggagacccag
     9181 gtattggtca tgccgatttc ttcgccgtta taaaggaagg gtgtgccgcg caggaagagc
     9241 aaaatggcca ggttgacccg ggcaatggcg gcatcattga caccatcacc aaactgggag
     9301 atcatgcggt ccacatcgtg gttgccgagg gtgttgcaag gccaggcatc ggggggcatg
     9361 ccagcggcac gttcctgttg attgtcccgg acccaggccg cgttgaggcg gcgagtgcgc
     9421 atgagcggga agttgaagac caggtggagc tcgtcttcac cgctgccgta gtaagtgagg
     9481 tcatcggttt cgccgaccag gacacggtcg ggatattcgt ttatcacggc gcgcagttcc
     9541 tgcaccagtt cgtgcatacc aggctgatca tgctgggcgg caaaaatgcc ttcccagagc
     9601 ttgccggaat tggcctgctc ttcgggggtt tgggccagcc ggttatagcg atagagatcg
     9661 tcaagggtga gctttgattg atggtctggc atggcgggat cttcaaagat cgtgccaatg
     9721 gcatccagac ggaagccatc cacgcccata tccagccaga agcgcaccgc cgaccacatg
     9781 gcctggcgaa cctccgggtt gtgccagttc aggtcgggtt gttctttgaa aaagaagtga
     9841 tagtaatatt catcggtctg ggggtcgtat tcccaggcgg ggccgccaaa ggtggcgcac
     9901 cagttattgg gcggctgccc gtttttgccg gggcgccaga cataccaatc ccgtttgggg
     9961 ttggtcttgc tggagcgtga ctcgatgaac caggggtgct ggtcagaggt gtgattaagc
    10021 accaggtcga gaatgaggta catgccgcgc tcgtggaggc cattcaggaa gcggcgaaaa
    10081 tcatcgaggg tgccgtattc aggggcgaca gcggtgtaat cggcaacatc gtagccgcaa
    10141 tcagccatgg gcgagggata gtgtggggaa agccagacgg catctacgcc gaggtctttg
    10201 aggtaatcca gttttgagat catgccagta aaatcgccaa tgccatcgcc gttgccatcg
    10261 gcaaagctgc gggggtaaat ttgataaaaa acggcgcgct gccaccattt tggagttgtc
    10321 atgcggttat tcctctcatc atcaaggttg agtgcgatta taccgcagac ccggaatgac
    10381 tttttatgat atatttggct ggttcccgtg aaattttaca gcccccatat ttgaccgggc
    10441 ttttcgtgcc tcatgcgagc atgaaaggcc gggctattct tttggagaaa aacagatgcc
    10501 cgtgatactg gaagcgcaac aattggttaa gaagttcaag gatttcaccg cagtcaaagg
    10561 tgtaagtttt gaaattcagg ccggtgaaat tttcagtctg ctggggccta acggcgccgg
    10621 gaagacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaat tcaggccggt
    10741 gaaattttca gtctgctggg gcctaacggc gccgggaaga cgacaaccat ctccatgctt
    10801 tccacgctgc tggcgcctac cagcggcgac gcgcagattg gcgggcactc ggttgtccat
    10861 gcaccaatgg cagtgcggca gttgattggg gtagtgccgc aggaaatcgc actgtatgac
    10921 gacctgaatg cggcggagaa cttgtttttc ttcggacgca tgcatggaat gggtggggct
    10981 gcgttaaaac agcgcgtggc cgaagtgctg gagcaggttg ggctgacgga tcgggccaag
    11041 agccgggtga agactttttc ggggggcatg aagcggcggt tgaatattgg ggtggggctg
    11101 ctgcaccgcc cacagatgat ttttatggat gagccgacgg tggggattga cccacaaagc
    11161 cgccgtaata ttctggacgc agtgaaagaa ttgaaccggc aagggttaac agtgctgtac
    11221 accacccatt acatggaaga ggcccaggaa ttatcagacc gggtgggaat tattgaccac
    11281 ggcgagctga ttgcgtgcgg gacccagaag gaactgacgc aactggtggg gcagcatgaa
    11341 actctgcggc tgcacctgaa tgatgaaatt aatgcggatg acctggcgca gaaattggaa
    11401 gccctgcccc agatcctcaa ggcatcggcg gcggaccatc aggtggtttt gatggtgaca
    11461 gaggccgagg aagcgctgcc aggcgtgatt gccacggcaa acgccctgaa tatcaagatc
    11521 aggtcggtgg atattgaaga gcccaatctg gaagcggtgt tcctgcattt gacgggccgg
    11581 gcgctgcgcg attaaggaat ccgggatgaa gaaaattttc aacattgcct tgaaggattt
    11641 actgatcaac tttcgggacc cgatggggct ggtcttgatg ctggtagcgc cgctggcgct
    11701 gacgttggtg attgcggcgg cgtttggcgg agtgggcggc ggcgggagtg ccggactggc
    11761 tgatattccg gttgtgattg tcaatcacga tacgggtgat ctgagccctt acctggttga
    11821 ggctttttat tctgaggaac tggccgaatt ggttgagccc agtacagtgg atgatgacgc
    11881 agccgcccgg gcggctgtgg atgccgatga ggttgccgcg gcggtgatca tcccggataa
    11941 tttttctgaa agtattctgc cggcagggct ggtgcagggc gatatatcca gcctggacag
    12001 caaaatagtc agcgtggtgg aggtttatgc caacccgggc cgtccggtca gtgcgactgt
    12061 gatccgcagc attgtggaca cggtcttgaa tcagtttgtg agcgggcgga ttggcagcga
    12121 gattaccatc aatcaattga ttgccagcgg caggctctct ccggctgaag cccagcagca
    12181 ggggatgatc attgggcaaa gcctgggagc ggcggaacca accgagcgga tcacattaag
    12241 cgaagagacc ggtacagcca gcagcggttt taactggctg gaatacagcg cgcccagtct
    12301 ggcgatcctg tttatgatgt ttacgttgtc ggcagccggg cgaacactgt tgattgaacg
    12361 cgatcacggc accctgcccc ggatgctggt cagcccgacc agccgggcag aaattctggg
    12421 cggcaagctg ctggggaatt ttctgacggg gctgttgcaa atgacgatct taattgtggc
    12481 ggggggattc ctgttcagga tatcctgggg cgacccactg gcggtggggc tgctgacact
    12541 ggcggtggtg gctgcggtga cgggatgggg aatgctgccg gctgcatttg cgcgcacacc
    12601 gggccaggcc aatgcgattg gaacagccat cacattgacg tttggggcgg cggcaggttc
    12661 ctttgtgcca cgcggggcgc tgcccgaatg gctgcaaaag gccgggctgg tgacgcccaa
    12721 tgcctggggc attgaggggt actacaacct catcaaaggg gcgacgctaa ccgagaccat
    12781 cccggccatt ctggcgctgc tggtgatggc aatcatcctg tttggcattg ccgtactggc
    12841 attccggcgg cagtacgcct gagagggaac catgcgaaaa atacttgccc tggcctggaa
    12901 tgatattcaa ctggagtttt cggaacgctc cacgctggta ttctttttga tcctgcccct
    12961 ggtctttacg gcgattattg gggtggcgct gcaaaataca tataccgacg gcagcgagga
    13021 tccacgcctg ccgttgattg tagtcaatga agatcaaggc acttatgcca ccgagttgat
    13081 ttctctgctg gatggctcgg acacggttcg agtggtggaa gcaagcgccc aggcggcaga
    13141 ggaaatgatt aacgatgatt ttccggcaat tttgaccatc ccggcggggt tcagtcaaaa
    13201 aatcagcacg ggagagcagg cgccgctgca attatataag tcaccggaca gcaacgctcc
    13261 ggttgggatt gaacaggcgg ttaaaaccgc ggcggcccgc ttgagcgctg cgctgaatat
    13321 cgcagaacaa tccaccgctg aggcggaaaa aatcaggccg tttgcaagcg agaccgaacg
    13381 acagacctat tttgaggaaa gcctggcagc cgcgcgggcg gcactggcta atccccccac
    13441 ccgctcggag acaacgcgct cggagaacgt taacactcag cagattgcca ctggttttga
    13501 gcaatcatca cccggacaac tggtgacatg ggtgatgatc acgctgctgg gtggggccga
    13561 ggtctttgtc aatgaacggt taaacggcac cctgcggcgg ctgctgacca cgccgacgac
    13621 aaagggaatc attatcaccg ggaagattgc cggacggctc atccttggga ttattcagat
    13681 ggttctgctg gtggggtttg gtgcggtggc gctgggcgtc aactggggca aatcgccggc
    13741 ggcgctggcg gttatgctgc tgagctttgg actggctgga actgcactgg gcgtgatgct
    13801 gggcgcgttt gcccgcaccc gcagacaggc cagcggactg accacgttat tctcgatgtt
    13861 gatggcggcg ttgggcggcg cctggtggcc gttggaagtg acaccaccgg cctatcaggc
    13921 tgcggtgaaa gtcattccga cctcctgggc gatgaccggg ttcactgatg tcattttgcg
    13981 cgggcagggc gtgagcggca tcctgttgga agccggggta ctgttgggct ttgcggcgct
    14041 gttcttcgca ataggcgtgt ggaagctgaa gtttgagtaa acggtaaccc ccaaccaaaa
    14101 ccatgcctgc caaagccaac cagagatttg atctggcccc ggctacagga ttacgccaga
    14161 gagaccgtct ggagtcaatg cgtcaaggcg gaccggggtg atttgattcc agaggcgctg
    14221 gggagacagg gcggtcacac gcagattgtt atccgtaagg ccatgcaagc gccagccctg
    14281 cgggccgagc gagtcggtgc tttcccacag gaccgggagg acctgcccca taaactggcg
    14341 gtgatagctt tcagcagcgg cggcaaagac cgcccgcagg cgggcgttgc gctgcttgcg
    14401 aacatccatg gtcggcagtc cgggcaggcg ctcggcgggc gtgcccgggc ggggcgagaa
    14461 ggtgaagaca tgcccaccgg caaattggat ttcttgaata aagtccagac tttcagcaaa
    14521 ctcggcttcg gtttcgccag ggaagcctac aatgacatcg gtggtgatgg cgatctccgg
    14581 ggcaacggcg cgagcagcct gtaccaggcc agagaaagat tcgggggtga ctttgcgcgc
    14641 catgcggcgc agtgtggcag cacagcccga ctgcagcggt aaatgtaaat gacggcagag
    14701 gcgcgggttt tgccagagct gccagaaatc tgggcccaga t
//
LOCUS       DAOPVS010000040        14487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4260_length_14487_cov_56.703645, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000040 DAOPVS010000000
VERSION     DAOPVS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4260_length_14487_cov_56.703645"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKL11_05155"
     CDS             <1..1096
                     /locus_tag="PKL11_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13024.1"
                     /translation="SFLIRANNIGTSAALLLIWLIKEWRCNGFKKTVNKALFVIGGML
                     LVLMPVLVYFAAIGTLDDMISASITYNFSYSFDARPEANNFNIFKSSLKPAFTFLRIW
                     IIFPTIGFILAIVHGARKLKEKRITVLEIALIIVWPVEILASSISGRGYSHYFISWLP
                     ILTLLSGYFFWNVDQFALSHNFSEIVNKRKPHLGFGIAILITLYIFSGSFNQYLETIN
                     LFVFNKNQEIEYTPKIANYISANTDQDDFVLVWGGQAGINLMSDRNSSTAYIYYPLYT
                     NSPLGITLQEKFYDDLVKNKPVLIVDAYIDAPDVLPSLDPKVRSEQIILYPLANNTQQ
                     SLDYITANYEQINQIDGYTVYRLKSENTVP"
     gene            complement(1160..1405)
                     /locus_tag="PKL11_05160"
     CDS             complement(1160..1405)
                     /locus_tag="PKL11_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNZ13025.1"
                     /translation="MDSGMIGKVEKAKRYAQERKRIHFHTFTVTMDGENNPHQIRYAD
                     GQWECDCSYFRTHKLCSHTMALEIVLEDMIPEKPPVG"
     gene            complement(1537..1998)
                     /locus_tag="PKL11_05165"
     CDS             complement(1537..1998)
                     /locus_tag="PKL11_05165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13026.1"
                     /translation="MSSALTPFEIQSAGLGDLAALAALERTCFSEDAWPLLDLVAALT
                     FPGMIRLKAVVDGRMVGFVGGDPHPGEGFGWITTIGVQPEFRGRGIGAALLAACENAM
                     AVPRVRLCVRRDNQSAIMLYEREGYQRTGVWQKYYNGGEDALVYEKNRLTF"
     gene            complement(2006..2911)
                     /locus_tag="PKL11_05170"
     CDS             complement(2006..2911)
                     /locus_tag="PKL11_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ13027.1"
                     /translation="MNDGTTAFVICAVAAIGLIVVAFLFSAIKIVAEYKRLVVFRLGR
                     CVGAKGPGLVLLIPVIDKSVSVDLREQVREIPAQTSITKDNAPISIDFLWYYRVFDPV
                     MSVLQVGNFEQAAQGIATTTLRAVIGGIPLDGVLSEREQINSALRTRLDEVTEKWGVK
                     VTNVEIREILPPRDVQDAMNRQLSAERNRRAVVTESTGTREAAINVAEGEKQSAILKA
                     EGEKQAAILKAEGEREAQALRAQGYALALDRIFAVAQSIDSKTLTLQYFETLRSMAAS
                     PSTKYIFPMEFTSLLGDFMQQRGNK"
     gene            complement(2952..3566)
                     /locus_tag="PKL11_05175"
     CDS             complement(2952..3566)
                     /locus_tag="PKL11_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNZ13028.1"
                     /translation="MNFLTDPNIAFVLLVSGFVLAVLALFAPGTGLVELGALFALALA
                     AYSLASLPINVWALVILILGVIPFVLAMRKTRHWVFLLIGIAALVVGSIFVFRNPEGG
                     LAVNPLLAGIVSLAAAAFLWLIGRKSLEAIGLKPVGNREAIIGKRGEARTDIYLEGTV
                     YVGGEEWSARSRTRIPIGSLVRVVSRDGLILDVEPVSPEELAAG"
     gene            complement(3715..4620)
                     /locus_tag="PKL11_05180"
     CDS             complement(3715..4620)
                     /locus_tag="PKL11_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13029.1"
                     /translation="MRYLAIRAENLTKFYGQHRGIEDLFLEVEPGEVFGFLGPDGAGK
                     TTTLKLLMAETRPTYGNALLLGMDAYRHSKAIHSRVGYLPANFRPGRWGTAQRYLTGL
                     AKVYGGESWLLAQELAAQFKLDLEQPLAAMSGEALTELGLVQAWMHRPDLLLLDEPAR
                     WLGNEALDVFYRLVRQARMEGRSVFLASSSVAEMERICDRVGVIHAGRLVTVERGLNL
                     RARALRKVEMRFAGPVSAEVFARLPNVEDIRLEENKLRCTLCGDPDALIKLASQFRVI
                     DIISQQPTLEEVFRRNYGIEAARAG"
     gene            5085..6878
                     /gene="metG"
                     /locus_tag="PKL11_05185"
     CDS             5085..6878
                     /gene="metG"
                     /locus_tag="PKL11_05185"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNZ13030.1"
                     /translation="MTETILIAVAWPYANAEIHVGNLTGSYLPADIAARYHRLRGRKV
                     LMVSGSDAHGTPITVRADAEGTTPVEVYQRFHAGFLDLFQQLGLTYDLFTSTHTTNHS
                     GIAQKIFCALLDNGYLHTQREFQWYSTAQNRFLPDRYVEGDCYICGYSGARSDQCDKC
                     GSLLDPAQLLNPRSRIDGSTPELRETEHYYLDLGKLQSQIVDFLRLRESYWRPNVLRQ
                     SLGQILADGLRPRPITRDLDWGIPVPLPDWGEGKCLYVWFEAVIGYLSASIEWSQLTG
                     QPEAWKDWWHNPATRSYYFIGKDNIPFHAIIWPGELIGVGGRFDELMGGKASSPLVLP
                     YDVPANEFMNLEGQKISGSRNWAVWARDFLTRYDPDPLRYYLTVAMPEAKDTDWSWED
                     FYKRNNDELVATWGNLVNRVLAFAYKHWEGIVPDPGELTPLDRELIASVEDGFQTVAI
                     EMEAVHLRAALSEAMRLAGEVNKYLDQTAPWQAIKTDRPAAARAIYTAMRAIDSIKVL
                     LSPFLPFTCERLHSFLGYTEPLFGTQSLSTLADDLGEHTVLRYHPNAAAVRWEPSQMQ
                     PGHIFNQPQPLFRKLEAKIIEEERARLGQSK"
     gene            6890..7597
                     /locus_tag="PKL11_05190"
     CDS             6890..7597
                     /locus_tag="PKL11_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13031.1"
                     /translation="MSRSKPIYLLVVIVLALAVMSCSLPGRVTAPTATEAPADSGLQS
                     PTEAPVVTEAPETDAQPADYAGVALTAQDLPAGFEELSDEDLEDMGISPDTFADAFAE
                     SLSKAEVQDFSAFLNVSDFEVIFDFVLAPVTLIERASFDLLLNDPTKAAEAFAEGAGV
                     PIEVVPMETVGDSSVLFTFTQASSGITLRVDVVISRRGEAVLITMVMYMDGQTPLVSA
                     HDAAVILDGKARAVQGK"
     gene            complement(7671..8306)
                     /locus_tag="PKL11_05195"
     CDS             complement(7671..8306)
                     /locus_tag="PKL11_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ13032.1"
                     /translation="MPTIEQARGWYPDMDAVHGFDHVLRVYAMAERLAEAEGADLEIV
                     RAAALLHDVEGSLPGSDDRPNHHHQSADFAAQVLEAEGWSKERIAAVQHCIRAHRYRS
                     DGERPQTLEAQVLFDADKLDVLGAIGAARTIAYAVIAGTPIHAAPSARFLTSGEEEPG
                     ELHSAYHEYLFKLRHVKARLHTPAALKIAEQRDAALRDFFEQLDAEMRGER"
     gene            complement(8293..9564)
                     /gene="hutI"
                     /locus_tag="PKL11_05200"
     CDS             complement(8293..9564)
                     /gene="hutI"
                     /locus_tag="PKL11_05200"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNZ13033.1"
                     /translation="MNTLLIHSASQVLTLAGGPQRGHELGRLSLIEDGAVLIEGETIA
                     AVGRSAELLAAYPKTPQLNAGGRAMLPGFVDPHTHLIWAGDRAAEFELRLQGKTYLEI
                     LAAGGGILSTVRATRTASLDELKAQTRRRAHALLAHGTTTAEAKTGYGLDHDSELVQL
                     QALLELDEEGPLELAPTYLGAHAIAPEYRDDPDGYTRLLCKQMLPAVRDWWARHAANR
                     PLPFVDVFCETGAFTLAQSREILETAKSLGFPLKIHADEFDNLGGASLAAELGAASAD
                     HLVKTSPEDITALAQSNTVAVSLPCTPFGLADPHYTPARAILEANGLLALATDINPGT
                     AWCENMQFAIALGCRYLRLTPAQAIAAATINSAAAIGRADRIGSIEVGKQADLILLQE
                     PDYRHLGYRFGANLVQTVIKRGKVVVDHANH"
     gene            9636..12074
                     /gene="gyrA"
                     /locus_tag="PKL11_05205"
     CDS             9636..12074
                     /gene="gyrA"
                     /locus_tag="PKL11_05205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ13034.1"
                     /translation="MTEIGLVQSVDIDEQMRTAYLDYAMSVIISRALPDARDGLKPVH
                     RRILFAMHELGIRSNTAYKKSARIVGEVLGKFHPHGDVAVYDAMARLAQDFSMRYPLV
                     DGQGNFGSIDGDPPAAMRYTEARLGILAEETLADIDKNTVDFADNFDGSLQEPTVLPS
                     RLPNLLLNGSSGIAVGMATNIPPHNLRNLAAAVNLMIEHYDNLDDLSVDDLMAAVPGP
                     DFPTGGVIIGREPIRQVYSTGRGRLTVRGMAHIEEIKGGRYAIIITEIPYQVNKTNLI
                     ERIADLAREGRVDSISDLRDESDRRGMSIVIELKRGAQPKQVLNQLYKYTPLQTTFSV
                     HLLALVEGEPRVLSLKRALQIYIEHRQQVITRRSQFELDKARARAHILDGLLIALANL
                     DEVIQTIRQAPDVETAKERLITRFKLTEIQAQAILDMQLRRLAALERQKIESEHRELL
                     AQIAYLEDLLTHPAKILHVIGTDLAELAEKHGDERRTRITAEAMEELKEEDLVQDEPV
                     LITLTQRGYIKRVSANLYRSQGRGGRGVTGQTVKTEDEVLMLIPARTVDTLLFFSDRG
                     KVYSEKVYQLPDAGRTAAGIPIVNILSLGPDERITAAVSVPNFEVAEFCTMATVRGRV
                     KRMALSELSSVRPSGLAAINLDSGDELGWVRLTSGSDDILLVTTQGQALRFAETEVRP
                     TGRAAGGVTGINLRGEDRVASMEVAEPGGFLLVVTTQGFGKRSALDEYPAKSRAGSGV
                     TTQDQKGFAKTGPVAVARVVQESDEVTFISTGGLVLRLKVADISLSSRATRGTYLMKL
                     PAGDAIASLARLRE"
     assembly_gap    12172..12271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12272..14439)
                     /locus_tag="PKL11_05210"
     CDS             complement(<12272..14439)
                     /locus_tag="PKL11_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ13035.1"
                     /translation="MDFISTLNPQQKAAVTAGLGQTLVLAGPGSGKTRVLTHRIAYLA
                     QAMGVRLFNIVAVTFTNKAAREMKNRVEHLLGESVEGLWIGTFHSICARLLRRESERL
                     PFKSDFVIFDADDQETLVKRAIKEMNIDEKLYRPASIHAAISAAKNELIPVEDYPTNT
                     YRNELIQRIYGRYEELLRASNAVDFDDLLLWTVKLLVDNPDVRERYGRRFQHVLVDEF
                     QDTNKAQYELLRLLASVHGNLFVVGDEDQSIYRWRGADYRNVLRFETDYPACQKILLE
                     QNYRSTQTVLNAARAVIDRNTQRTPKHLFSDRGTGSKLTLYEANDDHAEAAYVVDTIQ
                     RLLQRRAAKASDFAVMYRTNAQSRLLEEAFLHAGLPYRLVGAQRFYGRREVKDVIAYL
                     RLVQNPDDEVSLNRVINVPPRGIGDKTLVALQLAAQGAGVAPGAVLLSLGAQGQGSPY
                     WGSLARGAVSLADFGGLLGGWRAEREDTPLPALFDKILIETGYRSYLDDGSEEGKDRW
                     ENVEELRRLAYEFNDRGLTAFLENLALVSDQDTLPEKVEAPTLLTLHAAKGLEFGQVF
                     IVGLDEGVLPHSRALDDPEELAEERRLFYVGLTRAKDQVTLLRAEQRSSYGSPQMQQP
                     SRFLEDIPAELVVHEGLRHHGRSDSLRSWDVRPRQGATAAAAAPAGKYQANQKVRHPV
                     WGEGLVVHIRQGADGEETVDVFFESVGFKRLLVSMANLEVL"
BASE COUNT         2914 a         4672 c         3747 g         3054 t
ORIGIN      
        1 cagtttcctg atacgagcta acaatatcgg cacatcagca gctttgttgt taatctggtt
       61 aattaaagaa tggcgctgta acggattcaa aaagacggtt aataaagctc tttttgttat
      121 aggtggcatg ctgttggtcc tgatgccggt tttagtttac tttgctgcca ttggtacact
      181 ggatgatatg atctcagctt caattaccta taatttttct tatagctttg atgccagacc
      241 agaagcgaat aatttcaata ttttcaaatc cagcctcaaa ccggctttta cctttttgcg
      301 aatttggatc atctttccaa ctattggatt cattcttgca atcgttcacg gggcaagaaa
      361 actaaaagaa aaacgaataa ccgtgcttga aattgctttg ataattgtat ggcctgttga
      421 aatactggca agctcaattt caggtcgtgg ttatagccac tatttcattt cttggttgcc
      481 aatattgact ctgttgtcgg gatatttttt ctggaatgtc gatcaatttg cattgtcgca
      541 caatttttct gaaattgtga ataagcgaaa gccacatttg ggctttggca tcgcaatttt
      601 gataaccctg tatatttttt ctggcagttt caaccagtat ctagagacga tcaatctttt
      661 cgtttttaac aaaaatcagg aaattgaata tacccctaaa atcgccaact atatttctgc
      721 aaacacagat caagatgatt ttgtgttggt atggggtgga caagctggca tcaatttaat
      781 gagtgatcgg aattcttcca ctgcatatat ttactatcct ttatacacca attcgccatt
      841 aggcatcacc ctccaagaga aattttatga cgacttggtt aaaaacaagc ccgtcttgat
      901 tgtggatgct tatattgacg ctcctgatgt gcttccttca cttgacccca aagtacgcag
      961 cgagcaaatc atcctatacc cccttgctaa taatacacag caatcactcg actatattac
     1021 agctaactat gagcagatca accaaatcga tggctatact gtttatcgtc tgaaatctga
     1081 aaacaccgtg ccatagtgaa ataaatgcca ccatgcagtt attgcatggt ggcattatca
     1141 tagattagct ttttgggagt taccccaccg gcggcttctc ggggatcatg tcttccagaa
     1201 cgatctccag cgccatggtg tggctgcaca acttgtgcgt cctaaaatat gagcaatcgc
     1261 actcccactg tccgtccgca tagcgaattt gatgcggatt gttctcgcca tccattgtca
     1321 ccgtaaacgt gtgaaaatga atgcgcttgc gttcctgggc atagcgtttg gccttttcaa
     1381 ctttgccaat catgcctgaa tccattgggt ctgcatctcc tttcagtgag ttaggggaca
     1441 aaaaagcacg cgtacacacg cgtgcctgag ccgtcaatat ccaatttggg gggaggtaaa
     1501 tactcgcatc tcaataatga tgatataccg actcgctcag aaagtcaacc gattcttttc
     1561 ataaaccagg gcatcttcgc cgccgttgta atatttctgc cagactcccg tgcgttgata
     1621 cccctcccgt tcataaagca ttatcgccga ttgattatcg cgccgtacac ataaccgcac
     1681 gcgcggcact gccatggcgt tttcgcaggc tgccagcagc gctgccccaa tcccgcgccc
     1741 gcgaaactcg ggctgcacac cgatggtcgt aatccagcca aacccttcgc cggggtgcgg
     1801 gtcgccgcct acaaacccca ccatgcgccc atccaccacc gcttttaagc ggatcatccc
     1861 cggaaaggtc agggctgcca ctaaatccag caggggccag gcgtcctcgc taaagcaggt
     1921 gcgctcaagc gcagccagcg ccgccaggtc gcccagcccc gccgattgaa tctcaaaagg
     1981 tgtaagcgca gaactcacgc tgcgtttact tattgcctcg ttgctgcatg aaatcgccca
     2041 gcaggctggt gaattccatc gggaagatgt acttcgtaga tgggcttgcc gccatgctgc
     2101 gcagtgtttc aaagtattgc agagttaacg tcttggaatc aatgctctgc gctaccgcaa
     2161 agatgcgatc cagcgccagc gcataaccct gcgcccgcag cgcctgagct tcgcgctcgc
     2221 cttccgcttt gaggatggcc gcttgcttct cgccttccgc cttgaggatg gcgctttgct
     2281 tctcgccttc cgccacgttg atggcggctt cgcgcgtacc ggttgattcc gtcaccaccg
     2341 cgcggcggtt acgctctgcc gaaagctgcc ggttcatcgc atcctgaaca tcgcggggcg
     2401 gcaagatctc gcggatttcc acgttagtca ctttcacgcc ccatttttcg gtcacttcgt
     2461 ccagacgggt gcgcagtgcc gagttgatct gctcgcgctc agacagcaca ccatccaggg
     2521 ggatgccgcc gatgactgcg cgcagcgtgg tcgtcgcaat accctgcgcc gcctgttcaa
     2581 agttgccaac ctgcagcacg ctcatcacgg ggtcaaatac ccggtagtac cacagaaagt
     2641 caatcgaaat cggggcgtta tctttggtaa ttgaggtctg ggccgggatt tcgcgtacct
     2701 gctcgcgcaa gtccaccgat acagatttat caatcaccgg gatgagcaac accaaccccg
     2761 ggcccttggc gcccacacag cgccccaggc gaaagaccac cagccgtttg tattctgcaa
     2821 caattttgat tgctgagaat agaaacgcca caacaatcag cccaattgcg gcaactgcac
     2881 agatcacaaa tgccgtcgtg ccatcattca tttgaaacct ccatctatac tgaaaaaagg
     2941 ttgagatatg tttatccggc tgccaattcc tctggcgaaa ccggttcaac atcgagaatg
     3001 agcccgtcgc gcgagacaac ccgcaccagg ctgccaatcg gaatccgcgt ccggctgcgg
     3061 gcgctccact cttcgccgcc tacatagacg gttccttcca ggtaaatatc ggtgcgcgcc
     3121 tcgccccgtt ttccaataat ggcttcacgg ttccccaccg gcttgagccc aatcgcttcc
     3181 aggctcttac gcccaatcag ccataagaag gcagccgccg ccagcgagac gatgcccgcc
     3241 agcagggggt tgaccgccag cccgccttcc ggattgcgaa aaacgaaaat cgagccaaca
     3301 accagcgcgg caatcccaat cagcagaaaa acccagtgcc gggttttgcg catggccagc
     3361 acaaagggga ttactcccag aatgagtatc accagcgccc agacgttaat cggcagactt
     3421 gccagactgt aggctgccag ggccagcgca aacagcgccc ccagctccac caacccggtg
     3481 cccggcgcaa acagggccaa tacggccagc acaaatccgc tgaccagtaa aacaaaagca
     3541 atatttggat ctgtcaagaa gttcattttg cacctctcct gtatcattat atacgcaaaa
     3601 gcgcaatttg gtgtgtgccg cgcgtgtaaa atcgcccggc tgtggcactc tctgattaaa
     3661 taaacaggcc aggcttcatc aagcctgacc tgtaaacttc ttcccataac ccggctatcc
     3721 tgcccgggcc gcctcaatcc cataatttcg gcggaaaacc tcttccagtg tgggctgttg
     3781 gctgatgata tcaatcacgc gaaattgact cgccagcttg atcagcgcat cggggtcgcc
     3841 gcacagggtg cagcgcagtt tattctcttc caggcgaatg tcttccacat tgggcaggcg
     3901 ggcaaacacc tccgccgaga cgggccccgc aaagcgcatt tccacttttc gcagcgcccg
     3961 ggcccgcaaa ttcaggccgc gttccaccgt caccaaccgc cccgcgtgaa tcacccccac
     4021 gcggtcgcaa atccgttcca tttcagccac cgaactcgac gccagaaaga cggatcgccc
     4081 ttccatacgc gcctgccgca ccagccggta gaacacatcc aaggcctcgt ttcccagcca
     4141 gcgggcgggt tcatccagca gcagtaaatc ggggcggtgc atccaggcct gcacaaggcc
     4201 cagctcagtc agcgcctcgc ccgacatcgc cgccagcggc tgctccaggt ccagtttgaa
     4261 ttgggcagcc agttcctgcg ccagcagcca gctttcaccg ccatacactt ttgccagccc
     4321 cgtcagataa cgctgagcgg ttccccagcg ccccggccga aaattcgccg gcaggtatcc
     4381 cacccggctg tggatggctt tgctgtgacg gtaggcatcc atgcccagca gcagcgcgtt
     4441 gccataagtc ggccgggttt cagccatcaa gagctttaag gtcgtggtct ttccggctcc
     4501 atccggcccc aaaaagccga acacttcgcc cggttctacc tctaaaaata gatcttcaat
     4561 accccgatgc tgaccataga acttggtcag gttttcggcg cggattgcca ggtagcgcat
     4621 ggcagcctcc ttgaaaagtg gtaaatccgc ggcccttaaa aaagagcctc ggctggtatg
     4681 aagcaccagc cgagggaaac gtcaaatcac gcgctacgcg ccatttccat tagacccggt
     4741 tggggcttcc tgtaccggtt ttgccgaacc agcccaacac ccggcagggg gtgttcagct
     4801 cgttccgcca aaccttattt gtaacgaaca ggggtaacag gttcagtgtg cgcctaaggg
     4861 gcacactctc gccagaatta cccgactctt ccgaatccat acagaaactc cttgtagcgg
     4921 aacaaaacat agcttgccca ggcgggctgc cctcatttta gccgcttcac aggccaatgt
     4981 caagctgtca tctgtcctgt cattccttac aaaggtgtcg gctgccttca tctttcgtga
     5041 taaaatcgtg ctattcctgt aattccctca tctggaggtg cgtgatgact gagaccattc
     5101 tgattgctgt tgcctggccc tatgccaatg ccgaaatcca tgtcggtaat ctcaccggct
     5161 cttacctgcc ggcggatatt gccgcccgct atcaccggct gcgcggtcgc aaggtgttaa
     5221 tggtctcggg ctccgacgcc cacggcacac ccattaccgt gcgcgcggat gccgaaggca
     5281 ccaccccggt ggaagtgtat cagcgtttcc atgccggctt tctggacctc ttccagcagc
     5341 ttggcctgac gtatgatctc ttcaccagca cccataccac caaccacagt ggaattgccc
     5401 agaagatttt ctgcgccctg ctggataatg gttacctgca tacccagcgc gagttccagt
     5461 ggtactccac tgcgcaaaac cgcttcctgc ccgaccgcta cgtggagggc gactgctaca
     5521 tctgcggcta ttcgggcgcc cgctctgatc aatgcgataa atgcggcagc ctgctggacc
     5581 ccgcccagct gctcaacccg cgctcccgca ttgacggctc aacccccgag ctgcgcgaga
     5641 ccgagcatta ctacctcgac ctcggcaaac tccagagcca gattgtcgat ttcctgcgcc
     5701 tgcgtgaatc ttactggcgc cccaatgtgc tgcgccagtc gcttggtcag atccttgccg
     5761 atggcctgcg cccgcgcccc atcacccgtg acctcgactg gggcattccc gtacccctgc
     5821 ccgattgggg tgagggcaaa tgcctctatg tctggtttga ggccgtgatt ggctaccttt
     5881 ctgcctcaat tgaatggtcg cagctcaccg gtcaacctga agcctggaag gactggtggc
     5941 ataaccccgc cacccgctct tactacttca tcggcaaaga caatatcccc ttccacgcca
     6001 tcatctggcc cggcgaactg attggtgttg gcggccggtt tgatgaattg atgggcggca
     6061 aggcttcttc cccgttggtg ctgccgtatg acgtgcccgc caacgaattt atgaatctcg
     6121 aaggccaaaa aatctcaggc agccgcaact gggccgtctg ggcgcgtgat ttcctcaccc
     6181 gctacgatcc agaccccctg cgctactacc tcaccgttgc catgcccgaa gccaaagaca
     6241 ccgactggag ctgggaagat ttctacaaac gcaataacga tgaactggtc gccacctggg
     6301 gcaacctggt caatcgggtg cttgcctttg cctacaaaca ctgggaaggc atcgtgcccg
     6361 accccggcga gctgacgccc ctggatcgtg agttgatcgc ctctgttgaa gatggcttcc
     6421 agacggttgc cattgagatg gaagccgtgc atctgcgcgc ggccctgtca gaggccatgc
     6481 gcctggcggg tgaggtcaac aaatacctcg atcaaaccgc cccctggcag gccatcaaga
     6541 cggaccgccc tgccgctgca cgggccatct acaccgccat gcgcgccatc gattcgatca
     6601 aagttctgtt atcacccttc ctgcccttta cctgcgagcg cctgcacagc ttcctgggct
     6661 ataccgagcc gctttttggc acccaatccc tctcgaccct ggctgatgat ctgggtgagc
     6721 atacggtctt gcgctatcac cccaatgccg ccgccgtccg ctgggaaccc agccagatgc
     6781 agcccggaca tatctttaat caaccccagc cgctcttccg caagctggaa gctaaaatta
     6841 ttgaagaaga gcgcgcccgc ctggggcaat ctaaataaga ggaaaatcaa tgtctcgttc
     6901 taaacctatc tatctcctgg tcgtgattgt gctggcgctc gcggtgatga gctgcagcct
     6961 gccggggcgc gtcaccgcac ccacggccac agaagccccc gctgattccg gtctccaatc
     7021 gcccacagaa gccccggtgg taaccgaggc ccccgaaacg gatgctcaac cggcagatta
     7081 tgccggtgtg gcgctgaccg cccaggacct ccccgccggg tttgaagaac tgtctgatga
     7141 agatcttgaa gatatgggca tctctcccga cacatttgcc gatgcatttg cagagagttt
     7201 gtccaaggcg gaggtacagg atttctcagc cttcttgaac gtttcagatt ttgaggttat
     7261 ttttgacttt gtcctggctc cggtgacgtt gattgagcgc gcttcctttg acctgctgct
     7321 caacgatccc accaaggcgg cagaagcctt tgccgagggc gccggtgtgc cgattgaggt
     7381 cgttcccatg gagaccgttg gcgattcttc ggttttgttc acctttaccc aggcttcttc
     7441 aggcattacc ctgcgcgtgg atgtggtgat ttcccgccgc ggcgaggctg tgttgattac
     7501 catggtcatg tacatggacg gtcaaacccc actcgtctct gcccacgatg ccgccgttat
     7561 tctggatggc aaagcccggg cggttcaggg caaataagcg ccaaaaccag ttacgcagct
     7621 taaaaacacg tggcagtcct tggactgcca cgtgtgattt tgaccgatcc tcagcgttcc
     7681 ccgcgcatct cggcatccaa ctgctcaaag aaatcccgca gggctgcatc gcgctgttcg
     7741 gcaattttca gcgctgcagg ggtgtgcagc cgcgccttca catgcctcaa cttgaacagg
     7801 tattcatgat acgcactgtg cagttcgccg ggttcttctt ccccgctggt caaaaaccgc
     7861 gccgatggcg ccgcgtggat gggcgtccct gcaatgaccg cataggcaat cgttcgcgcc
     7921 gcgccgatgg cccccagcac gtccagcttg tcggcgtcaa acagcacctg tgcctccagt
     7981 gtttgcggac gctcgccgtc ggagcggtag cggtgcgccc ggatgcagtg ctgcaccgcc
     8041 gcaatccgct cttttgacca gccctcagcc tctaacacct gggcggcaaa atcagccgat
     8101 tgatgatggt ggttggggcg gtcatcgctg ccgggcaggc tgccttccac gtcgtgcagc
     8161 agcgccgcgg cgcgcacaat ctccaggtca gcgccttcgg cctctgccag ccgctctgcc
     8221 atggcataca cccgcagcac atggtcaaac ccatgcaccg cgtccatgtc cgggtaccag
     8281 ccccgcgcct gctcaatggt tggcatggtc caccaccact ttcccgcgct tgatgaccgt
     8341 ctgcaccagg ttggcgccaa agcggtaacc cagatgccgg taatccggct cctgcagcaa
     8401 aatcaggtcg gcctgcttgc ccacctcaat ggagccaatc cggtctgccc gcccaatggc
     8461 cgcggccgag ttgatggtgg cggcggcaat ggcctgcgcg ggcgtcagcc gcaggtagcg
     8521 gcagcccagc gcaatggcaa actgcatgtt ttcgcaccag gccgtccccg ggttgatgtc
     8581 ggtcgccagt gccagcaggc cgttggcttc cagaattgcc ctggccgggg tgtaatgcgg
     8641 gtctgccagg ccaaatggcg tgcacggcag cgagaccgcc accgtattgc tctgcgccag
     8701 tgcggtgatg tcttctggcg aagtcttcac cagatggtct gccgaggctg cgcccagttc
     8761 agccgccagg ctggccccgc ccaggttgtc aaactcatcg gcgtgaattt tgagcggaaa
     8821 gccgaggctt ttcgcggtct ctaaaatctc gcgagactgc gccagggtaa aggcgcccgt
     8881 ctcgcagaac acatccacaa aaggcagcgg gcggttggcc gcgtgccggg cccaccagtc
     8941 gcgcacagcg ggcagcatct gcttgcacag cagccgggtg tagccgtccg ggtcgtcccg
     9001 atattccggc gcaatggcat gggcgcccag gtaggtcggc gccagctcca gcggaccttc
     9061 ttcatccaat tccagcagcg cctgtaactg caccagctcg ctgtcatgat ccagcccgta
     9121 gccggttttg gcctcggcgg tggtggtgcc gtgtgccagc agggcgtggg cgcgccggcg
     9181 tgtctgcgct ttgagttcat ccaggctggc cgtgcgcgtg gcccgcaccg tcgagagaat
     9241 gcccccgccc gcggccagaa tttccaggta ggttttgccc tgcagccgca gttcaaattc
     9301 ggccgcccgg tcgcccgccc agatcaaatg ggtgtgtggg tccacaaaac ccggcagcat
     9361 ggcccgcccg ccggcgttca attggggcgt cttggggtag gctgccagca gttcggcgct
     9421 gcggcccacc gccgcaatcg tctcgccttc aatcagcacg gcgccatctt cgatgaggct
     9481 caaccggccc agctcgtggc cgcgctgcgg cccgcctgcc agcgtcagca cctgagatgc
     9541 agaatggatc aggagtgtgt tcatgcccaa aattataccc caaccttctc ccgccgcctt
     9601 gtgctataat tcggccttgc ttaactggag gctttatgac tgaaattggc ttggttcaat
     9661 ccgtggatat tgacgaacag atgcgcactg cgtacctcga ttacgccatg agcgtgatca
     9721 tctcgcgggc cctgcccgac gcccgcgacg gcctcaaacc cgttcaccgc cgcattctct
     9781 tcgccatgca cgaactgggc atccgctcca acacagctta caagaaatcc gcccgtattg
     9841 tcggcgaggt gctgggcaag ttccatcccc acggtgatgt agccgtttat gacgccatgg
     9901 cccgcctggc ccaggatttt tccatgcgtt atcccctggt ggatgggcag ggtaactttg
     9961 gctccattga tggcgacccc ccggcggcca tgcgttacac cgaggcccgc ctcggcattc
    10021 tggctgaaga aaccctcgcc gatattgata aaaacacggt ggactttgcc gataattttg
    10081 acggctcact ccaggagccg acggtgctgc cctcccggct gcccaacctg ctgctcaacg
    10141 gttcatcggg cattgccgtg ggcatggcga ctaacattcc cccgcacaac ctgcgcaacc
    10201 ttgcggctgc cgtcaacctg atgattgaac attacgacaa cctcgatgac ctcagcgtgg
    10261 atgacctgat ggcggctgtc cccggtcccg attttcccac tggcggcgtg attattggcc
    10321 gcgagcccat ccgccaggtt tattccaccg gccgcggccg cctgaccgtg cgcggcatgg
    10381 cccacattga ggaaatcaaa ggcgggcgct acgccatcat catcaccgaa atcccctacc
    10441 aggttaacaa aaccaatctg atcgagcgca ttgccgatct ggcccgcgaa ggccgcgtgg
    10501 attcaatttc tgatctgcgt gacgaatcgg accgccgcgg catgagcatt gtgattgaac
    10561 tcaagcgcgg cgcccagccc aagcaggtgc tcaatcagct ctacaaatat acccccctgc
    10621 aaaccacctt cagcgtgcat ctgctggccc tggtggaggg cgagccccgc gtgctgtccc
    10681 tcaagcgcgc cctgcagatc tacattgaac accgccagca ggtcatcacc cgccgctcgc
    10741 agtttgagtt ggacaaggcc cgcgcccgtg cccacatcct cgacggcctg ctgattgccc
    10801 tcgccaatct ggatgaggtc attcagacca tccgccaggc tcccgatgtt gaaaccgcca
    10861 aagagcgcct gatcacccgc ttcaagctga ccgaaattca agcccaggcc attctggata
    10921 tgcagctccg ccgcctggcc gcgctggaac gccagaagat cgaatccgaa caccgcgaac
    10981 tgctggctca gattgcctac ctggaagacc tgctgaccca cccggctaaa atcctgcacg
    11041 tcatcggcac cgacctggcc gagctggctg agaagcacgg cgatgagcgt cgcacgcgca
    11101 tcaccgccga ggccatggaa gaactgaaag aagaagacct ggtgcaggac gagcccgtgc
    11161 tgattaccct gacccagcgc ggctacatca agcgcgtgtc ggccaatctt taccgctccc
    11221 aggggcgcgg cggacgcggc gtcaccggcc agacggtcaa aaccgaagac gaagtgctga
    11281 tgcttatccc ggcccgcacg gtggatacgc tgctcttctt ctcggatcgt ggcaaggtct
    11341 attctgaaaa ggtgtaccag ctccccgatg ccgggcgtac cgccgctggc attcccattg
    11401 tcaatatttt gtcacttggc cctgatgaac gcatcactgc cgcagtgagt gtgcccaact
    11461 ttgaggtcgc tgaattttgc accatggcca ccgtgcgcgg gcgcgtcaaa cgcatggccc
    11521 tctctgaact gtcctcggtc cgcccctcgg ggctggcagc catcaacctc gattccggcg
    11581 atgagttggg ctgggtccgc ctgacctccg gcagtgatga tattctgctg gtcaccaccc
    11641 aggggcaggc gctgcgcttt gctgaaaccg aggtgcgccc cacgggccgc gccgccggcg
    11701 gcgtgacggg catcaacctg cgcggcgagg atcgggttgc ctcgatggaa gtggccgaac
    11761 ccggcggctt cctgctggtg gtcaccacgc agggctttgg caagcgctcg gccctggatg
    11821 aataccccgc caagagccgc gccggttccg gcgtgaccac ccaggatcaa aaaggctttg
    11881 ccaagaccgg ccccgtagcg gtagcccgcg tggtgcagga aagcgatgaa gtcaccttca
    11941 tctcaacggg tggactggtc ttgcgcctta aagtggccga catcagcctg agcagccgcg
    12001 ccacccgcgg cacctatttg atgaagctcc ccgccggtga cgctatcgcc tccctcgccc
    12061 gcctgcgcga atagccccgc ccttatcctg ccagcttatc cagtggagtg aaatctcccc
    12121 gctgctctct tagcgcgtct aaaatccagc cctccgcccc cacatctgga annnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagaacttcc agattcgcca tcgaaaccag
    12301 cagccgcttg aaccccaccg actcaaagaa cacgtccacc gtctcctcgc catcggcgcc
    12361 ctggcggata tgcaccacca acccctcacc ccagaccggg tgccgcactt tttgatttgc
    12421 ctgatacttc ccggccggcg ctgccgccgc cgctgtggcc ccctgccgcg gccgcacatc
    12481 ccagctccgc agtgagtcgc tccgcccgtg atgccgcaga ccctcatgca ccaccagctc
    12541 cgccggaata tcctctaaaa accgtgaggg ctgctgcatt tgcggcgacc cgtaactgct
    12601 gcgttgttcg gcgcgcagca gcgtcacctg atctttcgcc cgcgtcagcc ccacatagaa
    12661 caaccgccgc tcctccgcca gctcttcggg atcatccagc gcccggctgt gcggcagcac
    12721 cccctcatcc agccccacaa tgaacacctg cccaaattcc agccctttgg ccgcgtgcag
    12781 tgtcagcagg gtgggcgctt ccaccttctc cggcagggtg tcttgatctg acaccagcgc
    12841 cagattttcc agaaaagccg tcagcccgcg gtcgttgaac tcgtaggcca gccgccgcag
    12901 ctcttccaca ttctcccagc ggtctttgcc ttcttcgctt ccatcatcca ggtaactgcg
    12961 gtagcccgtc tcaatcaaaa tcttgtcaaa cagcgctggc agcggcgtat cctcacgctc
    13021 ggcgcgccag ccccccagca gcccgccaaa atctgccagt gaaaccgccc cgcgcgccag
    13081 tgacccccag tagggcgagc cctgcccctg ggcgcccagg ctgagcagca ccgcccccgg
    13141 tgcaaccccg gccccctgcg ccgccagttg cagcgccacc agcgtcttat cgccaatccc
    13201 gcgcggcggc acattgatca cccggttcag gctcacctcg tcatccggat tctgcaccag
    13261 ccgcaggtag gcaatcacat ctttgacctc acgccgcccg tagaaccgct gcgcccccac
    13321 cagccggtag ggcaggcccg cgtgcaaaaa ggcctcttcc agcaggcggg attgggcgtt
    13381 ggtgcggtac atcaccgcaa aatcactggc cttggccgcc cgccgctgca gcagccgttg
    13441 gatggtatcc accacatagg cggcttcggc gtggtcatcg ttggcctcgt agagcgtcag
    13501 cttgctgccc gtgccccggt ctgaaaacag gtgcttgggc gtacgctggg tgttgcggtc
    13561 aatcactgcc cgcgccgcat tcagcacggt ctgtgtggag cggtaattct gctccagcag
    13621 aatcttctgg caggccggat aatccgtctc gaaccgcagc acgttgcgat aatccgcccc
    13681 gcgccagcgg tagatggact ggtcttcatc ccccaccaca aacagattgc cgtgcacact
    13741 ggccagcagc ctcagcagct cgtactgcgc cttgttggta tcctgaaact catccaccag
    13801 cacatgttga aaccgccgcc cgtaccgctc gcgcacgtcc gggttatcca ccagcagctt
    13861 caccgtccac agcagcaggt cgtcaaaatc cactgcattg ctggcccgca gcagctcttc
    13921 ataccgcccg tagatccgct ggatcagctc gttgcggtag gtgttggtgg ggtaatcttc
    13981 caccgggatc agctcattct tggccgccga gatggccgcg tgaatgctcg ccgggcggta
    14041 gagcttctca tcaatattca tctctttgat cgcccgcttg accagcgttt cctggtcgtc
    14101 ggcgtcaaaa atcacaaaat cactcttgaa cggcagccgc tccgactcgc gccgcagcag
    14161 ccgggcgcag atcgagtgaa acgtgccgat ccacagccct tccacgcttt cccccagcag
    14221 gtgctccacc cggttcttca tctcccgcgc cgccttgttg gtaaaggtga ccgccacaat
    14281 gttgaacagc cgcaccccca tggcctgcgc cagataggca atccggtgcg tcagcacccg
    14341 cgtcttgcca gagcccggcc cggccagcac cagcgtctga cccagccccg ccgtcacggc
    14401 ggccttttgc tgcggattga gggttgagat gaagtccatg gcgccctcat tctacccgcg
    14461 cccccgccgc ctgtccagta cctcttc
//
LOCUS       DAOPVS010000041        14211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4365_length_14211_cov_58.245550, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000041 DAOPVS010000000
VERSION     DAOPVS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4365_length_14211_cov_58.245550"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKL11_05215"
     CDS             complement(<1..455)
                     /locus_tag="PKL11_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13036.1"
                     /translation="MADNRCPNCRSPLLPDAQVCPVCGKVLMETPPSGDDTPDWLREL
                     RGDMEAAPAEPAADTPDWLARIREDGQQNQPIEPDLTPAEPAEDVPDWLRDLSPAAAP
                     QSTPAESGDWLQALQSSGETPPPAETPAEPAAESDWLSEFRTSEPPAGG"
     gene            complement(461..1090)
                     /locus_tag="PKL11_05220"
     CDS             complement(461..1090)
                     /locus_tag="PKL11_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13037.1"
                     /translation="MRSPVSTAIAMAVGLLVLIGYLLPIGIVGQLRTIILDWAITLAG
                     IAGLVAIFNLAAVHWRKIGDKRNRDYYSIFLILAFWATFLAGVAAKVWGVPSQHDFQR
                     VVTAIQMPIEASLMGVLAISLAYASFRLFQRRKGLMAVIFVISALVFLIILSGLLSFG
                     GSVPVLGSLINLLNLLPVAGARGILLGIALGSLTTGLRILMGTDRPYSG"
     gene            complement(1087..1836)
                     /locus_tag="PKL11_05225"
     CDS             complement(1087..1836)
                     /locus_tag="PKL11_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13038.1"
                     /translation="MNLEHIAGLVLVLASVVLLIVLSLPRKTRRGRDLRDIPGFRRLR
                     RSIGLSVEEGSRLHISLGKSSPVNANGAASLAALAALERVARMNSLSDRPPIATSGDG
                     SLAILSQDTLKAAYRLANALELYDPDRGRLGGATPISYIAGALPITSTEHVGTHLLVG
                     SFGPEIALITEAAERRGALSLAATDSLPAQAALYATASEPLIGEELFAVSAYLQAGPA
                     HTASLRVQDVLRLVAVGALILGALLKLVGVL"
     gene            complement(1833..2162)
                     /locus_tag="PKL11_05230"
     CDS             complement(1833..2162)
                     /locus_tag="PKL11_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13039.1"
                     /translation="MAAVKSSGAWGANLFLKIRVLCYTPFMQGENPHWMIWAQFLQRW
                     GVKEIAAALLEAGGPLNIFMAQLAYAGGPLFSSQQPSGWQALAETLENPQQSRAFAAF
                     LRQEVKA"
     gene            complement(2125..2859)
                     /locus_tag="PKL11_05235"
     CDS             complement(2125..2859)
                     /locus_tag="PKL11_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ13040.1"
                     /translation="MHWIYLSPHLDDAVLSCGGIIWQQVQSGHQVEIWTICAADPPSG
                     PLAPFAQMLHARWQTGSQSMAARRAEDIQACRVLGAAWQHFPFADCIYRFDPQTGTPL
                     IADRDDLFAPLSTADLALADQLAADLAVQLTSGAHWVAPLTLGSHRDHQLVRRAAEQL
                     GAPLWLYADYPYAASDPDHATNLPANVQPQSFLLTPAALTVWQDAVAAYQSQLSSFWD
                     SEAQMRLALAEYYQNGGGQILWRLGC"
     gene            2888..3241
                     /locus_tag="PKL11_05240"
     CDS             2888..3241
                     /locus_tag="PKL11_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13041.1"
                     /translation="MNKSIPTWETGMNLLERLFGMPPYPPEAREEMRRLVDELVRIGI
                     EDDYLSERPGAPFNAQCRHVRAREIGKRLHDLGGLKLMVWTREAVGRKLGRRDALRLL
                     QHLDYAWAEIGEWMP"
     gene            complement(3246..>3806)
                     /locus_tag="PKL11_05245"
     CDS             complement(3246..>3806)
                     /locus_tag="PKL11_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13042.1"
                     /translation="AAALEFLEAIDNGEFTLQEELPAVTEAPGELLPAKSFNADQQII
                     WIYARDAADILRNAIQMVQRGERAFYRVAAVQLRILLCDTTRRHDAVVNIALLPYLYP
                     DLKLPQVNEDGEFDDALEPLPVETWLDQPLFIEDCGEVTLRQLIRRICDQDGGAHVDL
                     HPGTTLGPDAIDYIVYIGELILDRLF"
     assembly_gap    3807..3906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3907..5684)
                     /locus_tag="PKL11_05250"
     CDS             complement(<3907..5684)
                     /locus_tag="PKL11_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNZ13043.1"
                     /translation="MNQETAALHQPTLREMLALDVPVEVKLAPGGAWAAFLVRTMNWK
                     NNRYENVCYVQNLAAGGQTSPRRLNRSGSVQQMEWLNDDTLALLKTGNANGQPAQVWL
                     YEGLIGEGWQVTSHKEGVSSFRPFAGGLIFLADDPKRTEKKKRTERFGLYQHFEQEDS
                     AAALYYLNLSTLCQYEARQRTLTEDEAKDLVLPVVELSRLFDSPLHIGEMTPAPDGKS
                     IYLNCRRRDDYTYWRDTSVYCLQLDADAALAEYVRRAEEKKPADSTETSAETQAPAEE
                     KAAEPPEDLSYLGTLTRLNLPEQCFVVALSPDSTRLLLSFQGRDHHFYTQAELWTLDV
                     TAALAAPDASTARTQMRCLSANLDRFVLQAYWVQSGIFGSYVDGTVMRVARFDEHGQF
                     TVLDFDGLYVDDAFHICESGLIGLPAANSTSFSEACVARPGEGSHWSVQRLTDLGAQV
                     QGWDLGTVETIRWKSQDGVEIEGVLRKPSNFDPTQKYPLVFVVHGGPTASSPEYLLDY
                     DDRRYYPTIQFVNQGVLVLKPNYRGSDGRGQAFMELNVNNLGVGDLWDLESAIAHLDQ
                     LGWIDPQRVGCMGWSQGGYISAFAGLR"
     gene            5843..6415
                     /locus_tag="PKL11_05255"
     CDS             5843..6415
                     /locus_tag="PKL11_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNZ13044.1"
                     /translation="MTASTKAPARGNYTITDPPLIAALFGNTRWAWLWAILRVWLGYD
                     WITHGIDKLGNPAWMSGESLLGFWQRAVAVPEAPARPLIAFDWYRSFLQALIDGGSHV
                     WFAKFVAVGELLVGIALVLGIFTGIAAFFGGLMNFNFMLAGTASTNPVLFLAAILLIM
                     AWKVAGWWGLDRFLLPLLGTPWRPGRWFEK"
     gene            complement(6463..6996)
                     /locus_tag="PKL11_05260"
     CDS             complement(6463..6996)
                     /locus_tag="PKL11_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNZ13045.1"
                     /translation="MALDLTTLFPHESCVSSKLEGRVRPDGSRGVYALQPIAKGEILM
                     VWGGRIISAAELEKLPRELKQIVMQVEEDYYLSGLEEAVADWVNHSCNPNAGLSGQIV
                     LLAMRPIGPGEEICFDYAMCDGSCFDEFECDCGAPNCRRTITGNDWQNPELWERYAGY
                     FMPYLQRRIDRLKAAQS"
     gene            complement(7001..7837)
                     /gene="speE"
                     /locus_tag="PKL11_05265"
     CDS             complement(7001..7837)
                     /gene="speE"
                     /locus_tag="PKL11_05265"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNZ13046.1"
                     /translation="MNTSDLWVTEDLYGIYRSSMRYLELLHDEQTDFQHIQVYKTAYF
                     GNALLLDEIVQLTELDNAGYHEMMVHIPMLACPQPRRVLIVGGGDGGTLQQVLRYPSV
                     EQAVVCELDRRVVEVCADYFPAFGRPFDDPRAHLIIQDAFVYLQETPLKFDVIIADTT
                     DPVGEGEKLFSVEFYELMRRALAPGGAIVTQCEQIYFDQELIRGLIEIAQRLSQHPAY
                     YYAHVPTYPGGAIGFLYLSDTPWQNGLNKPYPPGTMNYLNPAVHQAAFALPEFFRRLL
                     ED"
     gene            complement(7834..8205)
                     /gene="speD"
                     /locus_tag="PKL11_05270"
     CDS             complement(7834..8205)
                     /gene="speD"
                     /locus_tag="PKL11_05270"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNZ13047.1"
                     /translation="MTNFLKLGEHFICELVDCNQTILLDNPRVRTLFVQAARQTGLSI
                     VGEGQFEFSPHGFTLYLLLQESHASIHVWPEYNYCAVDLFTCDLTAPATSFFDLLKQL
                     FEAGEMHVQMIERGVQQKVKI"
     gene            complement(8362..9183)
                     /locus_tag="PKL11_05275"
     CDS             complement(8362..9183)
                     /locus_tag="PKL11_05275"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNZ13048.1"
                     /translation="MFKIGEFSRLTWVPVKTLRYYDELGLLKPVEVDPFTSYRYYSAS
                     QLPRLNRILVLKDLGLTLEQIRLILEEDLTAEQLRGMLYRRQAELRQQIEEGQEMLAR
                     VESRLQQIDLEGRMTTMDVVIKRVPPMWVASVRGVIASYPEQGALWNELETALRLKGL
                     KPNGACFTIYHDEEYVDRNINAEVCEPVDGPAELSGRARIYELPGCETMACYIYHGPF
                     NGLSNAYSELLTWLSDNGYQISGVGREIYVHTGSGITRQDDPAYITEIQFPVTKA"
     gene            9347..10177
                     /locus_tag="PKL11_05280"
     CDS             9347..10177
                     /locus_tag="PKL11_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNZ13049.1"
                     /translation="MFELPEYTVLAQQMNATLTGKVIQQGQLGNSPHKFVWYNCSPEE
                     FTALTAGKRIGPARVQGRWLSLSLEPGYVLVLGECGGRVLYHPPQVELPEKYHLLIQF
                     TDGSALTTFTQMWGAMELYEAGREQERAYIKDMRVTPVETGFTFAYFSELIDDLLKGE
                     KRSTKSLLTQDQIIPGLGNAIAQDILFRARLHPRRALAELSPGQRRDLYTAIMDTVQD
                     VIAAGGRYDETDLFGKPGGYMRLMDSHAAGKPCPECGHTVEKIQYLGGACYFCPSCQV
                     "
     gene            10368..11060
                     /locus_tag="PKL11_05285"
     CDS             10368..11060
                     /locus_tag="PKL11_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ13050.1"
                     /translation="MNTSLAGQVYEAIKKDIISCALRPGQQIAQQQLAERYGAGITPV
                     REALQKLTREGLVLTIPRFGYLVTTITISDVAEIFELRAILESAAARLAAARASDEQV
                     EQLAGLAGYTYVYKDRDSYSDFLAHNRAFHIALANASGNKRLIEAISHLMDELTRMFH
                     LGLDLKDSAAEMRQEHQELVGVLRHRNPEEAEKIVLAQINRSRQRIYEALLPGNDNSP
                     DRIRQNIQLAQP"
     gene            11088..12107
                     /locus_tag="PKL11_05290"
     CDS             11088..12107
                     /locus_tag="PKL11_05290"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNZ13051.1"
                     /translation="MLTDHLPRVSLGFFPTPLEEMPRLAQLLGGPRLWIKRDDQTGLA
                     TGGNKTRKLEFSVAEALAQKADTLVTVGAVQSNHCRQTAAAAARYGLKCVLVIRGHAP
                     ENVTGNLLLDHLLGARIVFSGDRSREAAAEDVLAELKASGATPYFIPVGASNAVGAIG
                     FVAGMEELYHQLEALDLRADRIVFASSSAGTQAGLCVGAKMLGFRGQITGISIDSTRQ
                     ALSEEVAHIAAGVIQRLKVKMAFGPEEVVAYDGYLGAGYSIMGEPEREAIELAARYEG
                     LLLDPVYTGRAMAGLIDLIRKGEFAKDENIIFWHTGGAAALYAYANQLMGLPVKDQPT
                     GKLAK"
     gene            12142..13572
                     /locus_tag="PKL11_05295"
     CDS             12142..13572
                     /locus_tag="PKL11_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ13052.1"
                     /translation="MENLPYSADYEKAYAQFVQNYPEYLTTAKLDALRQTEYARLDRL
                     GMVYLDYTGGGLYAESQIQQHMLLLRDNVFGNPHSSNPSSMFATHLAERAREAVMQYF
                     NASPDEYVVIFTSNASGALKLVGESFPFCAGSRYLLTFDNHNSVNGIREFARARGAEV
                     TYIPVCLPELRVDESQIDDYLNLIKPGCQSLFAFPAQSNFSGVQHPLEWVERAHARGW
                     TVLLDGAAFVPTNRLDLSQVKPDFVPISFYKMFGYPTGIGCLVARKSALGLLRRPWFA
                     GGTITVASVQGDKYYLAPGEAAFEEGTLNYLGLPAVEIGLNYLQSVGLDIIHTRVHCL
                     TGYLLDSLSNLQDDLGHEGARIYGPIKMDRRGGTITLNFYDGHNHLVDHRKVEQRTNL
                     ANISIRTGCFCNPGAGEVALEISKPELITCFSQTTERLTLDDLRICVDGKNTGAVRIS
                     VGIASNFVDVYKFMGYVRELFGHPLE"
     gene            complement(13644..>14211)
                     /locus_tag="PKL11_05300"
     CDS             complement(13644..>14211)
                     /locus_tag="PKL11_05300"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNZ13053.1"
                     /translation="WNMKNKFLEQAKHEYSPKQRLIFLLPLAPIFLFLLPYLFVRLGF
                     GIDHWLQWPQILPLPYNYILGGLLILPGGVFAMWSIYSQFTLGRGTPVPLVATQQLII
                     QPPYTYCRNPMALGTILVYLGAAVLFHSPGGVVVVLLFSGLLLFYIKRVEEKEMVLRF
                     GQPYLAYKKSTPFIIPRFASGPRQNHPG"
BASE COUNT         3186 a         3675 c         4320 g         2930 t
ORIGIN      
        1 tcaccgccag cagggggttc tgaggtgcgg aactctgaca gccagtccga ttcggcggct
       61 ggctcggcgg gggtttcggc cggcgggggc gtctcaccgc tcgattgcag agcctggagc
      121 cagtcgccgg attcggcggg ggtgctctgt ggcgccgcag ccgggctcaa gtctcgcagc
      181 caatcgggca catcttcagc aggctcagcg ggggtcaggt cgggctcgat gggttggttt
      241 tgctgaccgt cctcacgaat gcgcgccagc cagtctgggg tatcggcagc gggttcggca
      301 ggggccgctt ccatatcgcc gcgtaattca cgcagccaat cgggggtatc gtcgccagag
      361 ggcggcgttt ccatcaagac tttaccgcaa accgggcaaa cctgcgcatc gggcaggagc
      421 ggggaacgac aattgggaca acggttatca gccataggga tcatccacta taagggcggt
      481 cagtgcccat gagaatacgc agcccggttg tcaggctgcc cagggcaatg cccagcagaa
      541 taccccgcgc cccggcaaca ggcaggaggt tgagaaggtt gatcagactg cccaggacgg
      601 gcacgctgcc gccaaaggat aaaagtccgc tcaaaattat caggaagacg agcgcgctga
      661 tgacgaagat gaccgccatc aggcctttgc ggcgctgaaa gagccggaaa ctggcatagg
      721 ccaggctgat cgccagcacg cccatcaggc tggcctcaat gggcatttga atggcggtaa
      781 caacccgttg aaaatcatgc tggctgggaa cgccccagac cttggcggca acacccgcca
      841 ggaaggtagc ccagaaggcc aggatgagga aaatgctgta ataatcgcgg ttgcgtttgt
      901 cgccaatctt gcgccagtgg acggcagcca ggttgaaaat ggcaaccagc ccggcgatgc
      961 ccgccagggt gatggcccaa tccagaatga tcgtgcggag ctgcccgacg atgccaatgg
     1021 ggagaagata gccaatcaga acgaggaggc ctactgccat ggcaatggcg gttgaaacag
     1081 gtgatctcat agcaccccca ccagtttgag cagggcgccc aaaatcaacg cgccaacggc
     1141 aaccaggcgc agcacgtcct gcacacgcag gctggccgtg tgggccgggc cagcctgaag
     1201 gtaggccgaa acggcaaaaa gctcttcgcc aatcaggggt tcgctggcag tggcatagag
     1261 cgcagcttga gcggggagcg aatcggtggc ggccaggctg agggcaccgc gccgttcagc
     1321 ggcttcagta atcaaggcaa tttcggggcc aaagctgccc accagcagat gggtgccaac
     1381 atgttccgtt gatgtgatcg gcagggcgcc ggcaatatag gatatgggcg tggcgccgcc
     1441 cagacggccg cggtcgggat catagagttc cagtgcgttt gccaggcggt aggccgcttt
     1501 gagggtatcc tgactgagga tagccaggga accgtcgccg ctggtggcaa tgggcgggcg
     1561 gtcgctgaga gaattcatgc gggcgacccg ctcgagggcg gccagcgctg ccagactggc
     1621 tgcgccgttg gcgttcaccg ggctggattt gcccagggag atgtgcaggc gggagccttc
     1681 ttctacagaa aggccaatag accggcgaag gcgtcgaaaa ccggggatat cacgtaaatc
     1741 tcggccgcgg cgggttttgc ggggcagcga gagcacaatc aacagtacca cgcttgccaa
     1801 aaccagcacc agaccagcga tatgttccaa attcacgctt tcacctcctg ccgcagaaag
     1861 gccgcaaagg cccgactctg ctggggattt tccaatgttt cagccagtgc ctgccagccc
     1921 gagggctgtt gggaactgaa cagggggccg ccggcatatg ccaactgagc catgaagata
     1981 ttcagggggc cgccagcctc caataacgct gcggcgattt ctttcacccc ccaacgttgc
     2041 aggaattgtg cccagatcat ccagtgtgga ttctctcctt gcataaatgg agtatagcat
     2101 aaaacacgaa tcttaaggaa cagattagca ccccaagcgc cagaggattt gaccgccgcc
     2161 attttgataa tattctgcca gggcgagccg catttgggct tcagaatccc aaaacgatga
     2221 aagctgggat tgataggccg ccacggcatc ctgccagacg gtgagggcag ccggggtgag
     2281 caggaaggac tgcggctgga cgtttgccgg gagattagtg gcgtggtccg ggtctgaggc
     2341 ggcatagggg taatcggcgt agagccagag cggggcgccc agttgttcgg cggcgcggcg
     2401 gaccagttga tggtcgcgat ggctgcccag ggtgaggggg gccacccaat gagccccgga
     2461 ggtaagctgc acggcaaggt cggcggcaag ctgatctgcc agggccaggt cggcagtgct
     2521 gaggggggca aaaagatcgt cgcggtcggc gatcaacggg gtgccggttt gcgggtcaaa
     2581 gcgatagatg caatcggcaa aggggaaatg ctgccaggct gcacccagca cgcggcaagc
     2641 ctgaatatct tcagcccggc gcgcggccat gctctgcgaa ccggtctgcc aacgggcatg
     2701 gagcatttgc gcaaagggcg ccagcggacc cgaaggcgga tcggcggcgc agatggtcca
     2761 gatttcaacc tggtgaccgg attggacttg ctgccagatg ataccgccgc aggacagcac
     2821 ggcgtcatcc agatgggggg aaagatagat ccaatgcata gtgatgtttt atcataaaaa
     2881 tgataaaatg aataaatcaa ttccgacctg ggagactggt atgaatctac tggaacgact
     2941 ttttggaatg cccccgtacc cgcccgaagc gcgggaggaa atgcggcgtc tggttgatga
     3001 actggtgcgg atcgggattg aggatgatta tctatctgag cgccccgggg caccttttaa
     3061 tgcgcagtgc cggcacgtgc gggcgcggga aattggcaag cggctgcacg acctgggggg
     3121 actcaagttg atggtgtgga cgcgcgaggc ggtggggcgt aagctgggcc ggcgcgatgc
     3181 cctgcggctg ctgcagcacc tggattatgc ctgggctgaa attggcgaat ggatgccgta
     3241 aacggttaaa acagccgatc cagaatcagc tcgccaatgt agacaatata atcaatggca
     3301 tcggggccca gggtggtgcc cggatgaaga tcaacatggg cgccgccgtc ttgatcgcag
     3361 atgcggcgga tcaactgccg taaggtcact tcaccgcagt cttcaataaa taggggctga
     3421 tcaagccagg tctcaacggg caacggttcc agggcgtcat cgaactcacc atcttcattt
     3481 acttggggga gtttcaggtc gggatacagg taagggagca gggcaatatt gacaacggca
     3541 tcgtgccggc gcgtggtatc gcacagtaag atgcgcagct gcaccgcagc cacgcggtag
     3601 aaggcccgct cgccgcgctg caccatttgg atggcgttgc gtaaaatgtc ggcagcatcc
     3661 cgggcataga tccagataat ctgttgatcg gcgttaaagg atttggccgg cagcagttcg
     3721 ccgggcgctt cggttaccgc cggaagttct tcctgcagtg taaattcacc gttatcaatg
     3781 gcttccagga attcgagggc cgcggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnncgga gcccggcaaa ggctgagata tagccgccct gcgaccaacc catgcagcct
     3961 acccgctgcg ggtcaatcca gcccaactga tccaggtgag cgatggcgct ttccagatcc
     4021 cacaggtcgc ccacacccag gttgttcacg tttaattcca tgaaggcctg accacggcca
     4081 tcagaaccgc gatagttggg cttgaggacc agcacgccct gattgacaaa ctggatggtc
     4141 gggtaataac gccgatcatc atagtccagc aggtattcgg gggagctggc agttgggccg
     4201 ccgtggacaa caaacaccag ggggtatttt tgggttggat cgaagtttga gggtttgcgc
     4261 agcacccctt caatctccac gccatcctgg cttttccaac ggatggtttc cactgtgccg
     4321 aggtcccagc cctgtacctg ggcgcctaaa tcggtcagac gctgaacaga ccagtgagat
     4381 ccctcaccag gccgggcaac gcaggcttcg gagaaagaag tggagtttgc ggctggaaga
     4441 ccgatgaggc cggattcgca gatatggaag gcgtcatcca catacaggcc atcaaagtcc
     4501 agcacggtga actgcccatg ctcgtcaaag cgagccacgc gcatcacggt accgtccaca
     4561 taactgccaa aaatgccaga ctgcacccag taagcctgca gcacgaaacg atcaagattg
     4621 gcggacaggc agcgcatttg cgtccgggca gtggacgcat ctggggcagc cagggccgcg
     4681 gtcacatcca gcgtccacag ttcagcctgg gtgtagaaat gatggtcacg cccctggaat
     4741 gagagcagca gccgggtgct gtctggagac agggcaacaa caaagcactg ctcaggcagg
     4801 ttgaggcgcg tgagtgtgcc caggtaagaa aggtcttcag gcggttcagc ggctttttct
     4861 tcagccgggg cctgggtttc ggcagaggtt tcggtgctgt cggccggctt tttctcttcg
     4921 gcgcggcgga catactctgc aagggcagca tctgcatcaa gctgcaggca atatacgctg
     4981 gtatcgcgcc agtaggtata atcatcacgg cggcggcagt tgaggtaaat ggatttgcca
     5041 tcgggagcgg gggtcatttc gccaatgtgc agggggctgt caaagaggcg gctgagttcc
     5101 accaccggca gcaccaggtc tttagcctcg tcttcggtaa gggtgcgctg ccgggcctcg
     5161 tactgacaca gggtagacag gttcaagtag tacagcgcgg cggcgctgtc ttcctgttca
     5221 aagtgctgat acagtccaaa gcgctcggtg cgcttttttt tctctgtccg cttggggtcg
     5281 tcggcaagga agatcaggcc gccggcaaag ggtcgaaagc tgctgacgcc ctccttgtgg
     5341 ctggtgacct gccagccttc gccaatcagt ccttcgtaca gccagacctg agccggctga
     5401 ccgttagcgt taccggtctt gaggagcgcc agggtgtcat cattcagcca ttccatctgc
     5461 tgaacgctgc cgctgcgatt taagcggcgg ggagaggttt gtccgccggc ggccaggttc
     5521 tggacataac acacattctc gtagcggtta tttttccagt tcatggtgcg caccaggaac
     5581 gcggcccagg cgcctccggg cgcaagtttg acttcgacgg gcacatccag cgccagcatc
     5641 tcgcgcaggg tgggttggtg aagcgcggcg gtctcttgat tcatggtgtt gaactcctat
     5701 gggctttggt gacagacttc catcattata gccaattttt agctgttgta accactttgt
     5761 tatatttgca tatcatttgt aattgtgata atatagataa taagataatt tttatccgta
     5821 tatcacaaaa ggacaggaac atatgactgc atcaactaaa gctccggctc ggggcaacta
     5881 taccatcacc gacccgccgc tgattgcggc gctgttcgga aatacccgct gggcctggct
     5941 ctgggccatt ttgcgcgtct ggctgggtta tgactggatt acccacggca ttgacaagct
     6001 gggcaacccg gcctggatgt ctggcgaatc gctgctgggt ttctggcagc gcgcggtggc
     6061 cgtccctgag gcgccagccc gcccgctgat tgcgtttgac tggtaccgca gctttttgca
     6121 ggcgctgatt gacggcggtt cgcacgtctg gtttgccaag tttgtggccg tcggcgaact
     6181 gctggtgggc attgcgctgg tgctgggcat cttcaccggg attgcggcct tcttcggcgg
     6241 actgatgaac ttcaacttta tgctggccgg aactgccagc accaacccgg tgctcttcct
     6301 ggcggcaatt ctgctgatta tggcctggaa agtggctggc tggtgggggc tggaccgctt
     6361 cttactgcct ctgttgggga cgccctggcg acccggcagg tggttcgaaa agtaaaaata
     6421 aaatgccctg gcaaatcagg ccagggcatt ttgctgtcta tatcaggatt gggcagcctt
     6481 caggcggtca atgcggcgct gaaggtaggg catgaaataa ccagcataac gctcccagag
     6541 ttcgggattc tgccaatcgt tgccggtgat ggtgcggcgg cagttggggg cgccgcagtc
     6601 acattcaaat tcatcaaagc agctgccatc gcacatggca taatcgaagc agatttcttc
     6661 gccgggacca ataggacgca tggccaggag gacaatctga ccgctgaggc cagcgttggg
     6721 attgcaggag tgattgaccc aatcggcaac ggcttcttca aggccagaga ggtaatagtc
     6781 ttcttcaacc tgcatcacaa tttgcttgag ttcgcggggc agcttttcca gttcagcggc
     6841 agagatgatg cgtccacccc agaccatcaa gatttcgcct ttggcaatgg gctgcagggc
     6901 gtacaccccg cggctgccat ccgggcgcac acggccttcc agtttggacg aaacgcagga
     6961 ttcgtgcggg aaaagcgtgg taaggtccag agccatgggg ttaatcctcc agcaggcggc
     7021 ggaaaaattc gggcagggca aaggcggcct gatgcactgc cgggttcagg taattcattg
     7081 tgccaggggg gtagggtttg ttcaggccgt tttgccaggg ggtatcggat agatacagga
     7141 agccaatggc gccgcccggg taggtgggga cgtgggcata gtaataggcc gggtgctgcg
     7201 acaggcgttg agcaatttct atcaggccgc ggatcagctc ctgatcaaaa taaatttgct
     7261 cacactgggt gacgatggcg ccgccgggtg ccagggcgcg gcgcatcagc tcgtaaaatt
     7321 cgaccgagaa gagcttttca ccctcgccaa ccggatcggt agtatcggcg ataatcacat
     7381 caaacttgag gggggtttcc tgcagataga caaaggcatc ttgaatgatg aggtgggcgc
     7441 gcgggtcatc aaacgggcgg ccaaaagcgg ggaagtaatc ggcgcagacc tcgactaccc
     7501 ggcgatccag ttcacagaca acggcctgct caacggatgg gtaacgcagt acctgctgca
     7561 atgtgccgcc atcaccaccg cccacaatca acacccggcg cggctgcggg caggccagca
     7621 tggggatgtg gaccatcatt tcgtgatagc cggcattatc cagttcggtt agctggacga
     7681 tttcatccag caacagcgca ttgccaaagt aagcggtttt atagacttga atgtgctgaa
     7741 agtcggtctg ttcgtcgtgc agcaattcta aataccgcat cgatgagcgg tagatgccgt
     7801 acaggtcttc ggtaacccat aagtcggagg tattcatatc ttcacctttt gctgaacgcc
     7861 gcgctcaatc atctggacgt gcatttcgcc agcttcaaat aattgcttca ggagatcaaa
     7921 aaaagaggtc gccggggcgg ttaagtcaca ggtgaagaga tcaactgcgc agtagttgta
     7981 ctcgggccag acatgaatgc tggcatggga ttcttgcagc agcaagtaga gggtgaagcc
     8041 gtgagggcta aactcaaatt gaccttcgcc aacaattgag aggccagttt gccgggcggc
     8101 ctggacaaat aatgtccgta cgcggggatt atctaacaga atggtctggt tgcagtcaac
     8161 cagttcacag ataaagtgct cgccaagttt caggaaattg gtcaccctta tctgcaccct
     8221 cctttttttg gagatgtgca gcggcggaat tttcgccgca ttgcggttga gagtatagca
     8281 tattttcccc acatgtaaat ataactcaac ggcctggcgg gcaaatgacc cgccaggccg
     8341 agtatcaggc gctgagagaa atcaggcttt ggtgaccggg aactggatct cggtaatgta
     8401 ggcggggtca tcctggcggg tgatgccgct gccggtgtgg acataaatct cacgacccac
     8461 accgctgatc tgatagccat tatccgaaag ccaggtcagc agctcagagt aggcattgga
     8521 cagcccattg aacgggccat ggtaaatgta gcaggccatc gtttcgcagc cgggcagttc
     8581 ataaatccgg gcccgcccag aaagctctgc cggaccatcc acgggttcgc atacttccgc
     8641 attgatgtta cgatccacat attcttcatc gtggtagatg gtgaagcagg ccccattggg
     8701 cttgaggccc ttaagccgca gggcggtttc cagttcgttc caaagcgcac cctgttctgg
     8761 ataggaggca attacgcccc gaactgaggc cacccacatg ggcggcacgc gcttaatcac
     8821 aacatccatt gtcgtcattc gtccctccaa atcaatttgt tgcagccggg attccacacg
     8881 ggctaacatt tcctggcctt cttcgatctg ctggcgcagt tctgcctggc ggcggtacaa
     8941 catgccgcgc agttgttcgg cagtcaggtc ttcctcaagg atcagacgaa tctgctcgag
     9001 ggtcagacct agatctttga gaaccaggat ccgattgagg cggggaagct ggctggctga
     9061 gtagtagcgg tagctggtga agggatctac ctcaaccggt ttgaggaggc ccaactcgtc
     9121 ataataacgg agtgtcttca ccggcaccca ggtcaaacgt gagaactcac caatcttgaa
     9181 catagatcct ccggaggcaa ttgcacgtag atgatatacc ctccagcagg gggagagtca
     9241 agggggcaat tgcgccaatt ttgaaagaag ttgacctctt gacgaaatag aacaaaaggc
     9301 ctattatcgg attattccac cgattgactt gagaaagagg ttcagcatgt ttgagttacc
     9361 tgaatatact gtgctggcgc agcaaatgaa cgcgaccctg acgggcaaag tcatccaaca
     9421 ggggcagttg gggaattctc cgcacaaatt cgtctggtat aactgctcgc cggaggaatt
     9481 tacggccttg actgccggga aacgcattgg gccggcacgg gtgcaggggc gctggctttc
     9541 gttgagcctg gagccgggct atgtgctggt gttgggtgag tgcggcgggc gggtgctcta
     9601 tcacccaccc caggtagagc tgcccgaaaa atatcacctg ttgattcagt tcacggatgg
     9661 ttctgcactg acaaccttta cacaaatgtg gggcgccatg gagctttacg aagcaggcag
     9721 agagcaagag cgcgcctata tcaaagacat gcgagtgacg ccagtggaga ccgggttcac
     9781 gtttgcgtat ttttctgagc tgattgatga cctgctgaaa ggcgaaaaac gcagcaccaa
     9841 aagcctgctg acgcaggatc aaattatccc cgggctgggg aatgcgattg cacaggatat
     9901 tctctttagg gcgcggctgc acccgcgacg ggcacttgcc gaactatcgc caggacagcg
     9961 gcgggacctg tacacagcca ttatggatac tgtgcaagat gtcattgctg ccggtgggcg
    10021 ctatgacgaa actgacctgt ttggcaagcc ggggggctat atgcgcctga tggacagcca
    10081 tgcggcgggc aaaccctgcc ccgagtgcgg gcacacggtt gaaaagattc aatatctggg
    10141 cggcgcctgt tatttctgcc ccagctgcca ggtttagcac cagcaccagt ttctgaccat
    10201 caccccggct gccagaatga ctggcagccg gttttatcct gtgagggatg acatgacatt
    10261 tgggggagac tctgctattt acagttgggg agcagcatga tattcttata atatatcact
    10321 gatatatcag agccctgccc tgctttcttc taattacagg tgaacccatg aatacatccc
    10381 tggctggaca agtctatgag gcgatcaaaa aagatatcat cagttgcgcg ctcaggccgg
    10441 ggcagcaaat tgcccagcag caactggccg agcggtatgg tgcgggcatc acgccggtgc
    10501 gagaagcgct gcagaagctg acccgtgaag ggctggtgct gaccatcccg cgctttgggt
    10561 acctggtgac aacaattacc atttctgatg tagcagaaat ctttgagctg cgggcaatcc
    10621 ttgaatcagc ggcggcgcgg ttggccgctg cgcgggcgtc tgacgagcag gttgagcagc
    10681 ttgccgggct ggcaggctac acttacgttt ataaagaccg agacagctac tcggattttt
    10741 tggcacacaa ccgggcgttt cacattgcgc tggctaatgc cagcggcaac aaacgcctga
    10801 ttgaggcaat ctctcatctg atggatgaat tgacgcgcat gttccacctg ggattagacc
    10861 tgaaagacag tgcggcggag atgcgccagg agcaccagga actggtgggg gtattgcgcc
    10921 ataggaaccc ggaggaagct gaaaagattg tgctggcaca aattaaccgc tcgaggcagc
    10981 gcatctacga agctttgctg cccggcaatg ataacagccc ggatcgtatc cggcaaaata
    11041 ttcaactcgc tcaaccctaa ttttcacata tctcaaggag gatcagcatg ctcacggacc
    11101 atctgccccg cgtatcgctg ggatttttcc cgacaccttt ggaagaaatg ccgcgtttgg
    11161 ctcaactact gggcgggcca cggctgtgga tcaagcgtga tgatcaaacc gggctggcaa
    11221 ccggcggtaa caagacgcgc aagctggaat tcagcgtggc tgaagcactg gctcaaaagg
    11281 ccgataccct ggtgacggtg ggcgcggtgc agtccaatca ctgccggcaa acagccgcgg
    11341 cggcggcgcg ttacgggttg aagtgtgtcc tggtaattcg cggacacgcg ccggaaaacg
    11401 tgacaggtaa cctgctgctg gatcacctgc tgggggcaag gatcgttttt tcaggggacc
    11461 gctcccgcga agctgcggct gaagatgtgc tggctgaact gaaagccagc ggggcaacgc
    11521 cctacttcat cccggtgggg gcatctaatg cggtgggagc aattggtttt gtggcgggga
    11581 tggaagagtt gtaccaccaa ctggaagcgc tggacctgcg ggcagatcgg attgtgtttg
    11641 ccagcagttc ggcgggcacc caggcggggt tatgtgtggg cgccaaaatg ctcgggtttc
    11701 gcggccagat tacggggatt tcgattgaca gcacccggca agcattgagt gaagaagtgg
    11761 cgcacattgc ggcaggggtc attcagcggc tgaaggttaa gatggctttt ggaccggaag
    11821 aagtggtggc ctacgatggc tatctgggag ccggttactc gattatgggc gagccagaac
    11881 gggaagccat tgaacttgcg gcacggtatg aaggccttct gctggacccg gtgtacaccg
    11941 ggcgggccat ggccgggctg attgatttga tccgcaaggg tgaatttgca aaggatgaga
    12001 atatcatttt ctggcacacc ggcggagccg cagcattgta tgcctatgcg aatcagttga
    12061 tggggctgcc ggtcaaagat cagcccaccg ggaaattagc caaataaaga ccacgttttg
    12121 caggtagaaa aggatcaccc catggaaaac ctcccctatt cagccgacta cgaaaaagcc
    12181 tatgcacagt ttgtgcaaaa ttatcccgaa tatctgacca ccgcaaaact ggatgcgttg
    12241 cgccagaccg aatatgcccg cctggacagg ctggggatgg tgtatctgga ttataccggc
    12301 ggcggcttgt atgctgaatc acagattcag cagcatatgc tcctgctgcg ggataatgtt
    12361 tttggaaatc cgcactcgtc caaccccagt tcaatgtttg cgacacatct ggctgaacgg
    12421 gcgcgcgagg ctgtgatgca atacttcaat gcctcaccgg acgagtatgt ggtcattttt
    12481 acctccaatg ccagcggtgc gctgaagctg gtgggggaat ctttcccctt ctgtgcaggc
    12541 agccgctacc tgctgacgtt tgataatcac aattcggtga acggcattcg cgagtttgcc
    12601 cgagcccggg gggcagaggt cacatatatc cccgtctgcc tgcctgaact gcgggtggat
    12661 gaaagccaga tcgatgatta cctgaacctg atcaaaccgg gctgtcagag tctatttgct
    12721 ttcccggcgc aatccaattt ctcgggggtg cagcacccat tggaatgggt ggagcgggcc
    12781 catgccaggg gttggactgt gctgctggat ggcgcggcct ttgtgccaac caaccggctt
    12841 gatctaagcc aggtgaagcc cgattttgtg ccgatttcct tctataagat gtttggctac
    12901 cccaccggga tcggctgtct ggtggcgcgc aaatcggcgc tgggattgct gcggcgtccg
    12961 tggtttgcgg gagggacaat caccgtggcc tcggtacagg gcgacaagta ttacctggcg
    13021 cctggcgaag cagcttttga agaaggcaca ctcaactacc tgggactgcc agccgttgaa
    13081 attgggctga attatctgca gtcggtggga ctggatatca tccacacccg ggtgcactgc
    13141 ctgacgggtt acctgctgga tagtctctca aatctacagg atgatctggg tcatgagggc
    13201 gcccggattt acggaccgat caagatggac cggcgtgggg gaaccataac actgaatttt
    13261 tacgacggcc ataatcatct ggtggatcac cgcaaggtgg agcagcgcac caatctggcg
    13321 aatatttcca ttcgcaccgg gtgtttctgc aaccctggcg ccggggaagt ggcactggag
    13381 atctcaaagc ccgaattgat tacctgtttc tcgcaaacca ctgaacggct gacgctggat
    13441 gatttgcgta tttgcgtcga tggcaaaaat accggggcag tgcggatttc ggtaggtatt
    13501 gccagcaatt ttgtagatgt ctataaattt atggggtatg tgagagagtt gttcgggcat
    13561 ccgttggagt aatgaaaaag ccccggcaga atattatctg ccggggcttt tttgattccc
    13621 aattattgaa ctctcgctgt tgattaaccg ggatgattct gccgggggcc tgaagcaaag
    13681 cgaggaatta taaaaggtgt gcttttctta tatgccagat aaggttgccc aaagcgaagc
    13741 accatctcct tttcctcgac gcgtttgata taaaacagta agaggcccga aaagagcagc
    13801 acaacaacaa ccccacccgg cgaatggaaa agcacagccg cgcccagata gacaaggatg
    13861 gttcccaaag ccatgggatt tcggcaatag gtgtatggtg gttggataat caattgctga
    13921 gttgctacca atggtacagg tgtgccccgc ccaagcgtaa attggctgta gattgaccac
    13981 attgcaaaca ccccacccgg caggatcaac aacccgccca ggatgtagtt ataggggaga
    14041 ggaagtatct gcggccactg aagccaatgg tcaatcccaa aacccaggcg gacgaagagg
    14101 tagggcagca gaaacaagaa gatgggagct aaaggcagga gaaagatcag tcgctgcttt
    14161 ggactgtatt catgcttagc ctgctcaaga aacttgtttt tcatattcca t
//
LOCUS       DAOPVS010000042        14170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4381_length_14170_cov_58.221962, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000042 DAOPVS010000000
VERSION     DAOPVS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_14170_cov_58.221962"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKL11_05305"
     CDS             <1..228
                     /locus_tag="PKL11_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13054.1"
                     /translation="LFITHDIDLAVVYANRVIVVNNGQIVRDGSPVEVLKDMDMLAQN
                     RLVPTSLLRLNLERYPRTQHFQRAEDLAHLQ"
     gene            273..1205
                     /locus_tag="PKL11_05310"
     CDS             273..1205
                     /locus_tag="PKL11_05310"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNZ13055.1"
                     /translation="MKNKAVTVVLALLAVLAFFVGIWQIGRLINPSLDLDETALLRFV
                     FVGIGLLIVFVVYYLTRQSKIWEVGTREVVYMAIGAALYALFSYIFNGSVFVVPSVSQ
                     VALRPAISIPMFFGFAFGPVVGFFTGAVGNMFGDALTGFGLSPQWSVGNGLVGMIAGL
                     WMLFKDKQKSMNIILIVSAVLALLATGIFLLNRTLPNMLFFDVDNGIFGDQQISIFAG
                     LTAVIGFALVLLVRFLFKNNVDVAAAVTWSMLGNLLGIGFAAVSDIWINGFSPAVAIV
                     GEFIPSAGPNLIFAAILVPILVAAFAAVRRQSGR"
     gene            1270..2136
                     /locus_tag="PKL11_05315"
     CDS             1270..2136
                     /locus_tag="PKL11_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNZ13056.1"
                     /translation="MLVTWRYRIRKSIIQFFDPRAWVIFFICFLASVLAFWDLRYLLF
                     FFVLTVGITLAADVSWAEIRRAVLFIGGFILFFAFLTFLTGRGGMELYSEEHILREFR
                     APFDLFGWVPTLKITAERIMYAVSMMARVFSIAIMTILIPYSLNPALYGVTFRGLGLP
                     DKIAYAMDLTMRFIPTYGRDFTLTMDAQRARGFELDKVKGGLFAQVRKLAPLVVPVTI
                     HAIAGSEDIIDAMDLRAFGVGPRTWLDELKFHWYDWVLIGLGVLMLLFSTALALFGYG
                     KFWVPNFLLPGS"
     assembly_gap    2152..2251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2252..2611
                     /locus_tag="PKL11_05320"
     CDS             <2252..2611
                     /locus_tag="PKL11_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ13057.1"
                     /translation="WPGPVEGERPSAYIIVLGDKEIAQNFNIDPGIAVQTILLGAVAQ
                     GLGGCIIASVNRKELIPLLQIPDRYEVLFVLALGKPVEEVVVDPIAPSGDIKYWRDEK
                     HVHHVPKRALDDLIVDL"
     gene            2775..5153
                     /locus_tag="PKL11_05325"
     CDS             2775..5153
                     /locus_tag="PKL11_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNZ13058.1"
                     /translation="MKPKSLIAFLLGLLLTLVWVIPVQADGIVIPGCPPDGCQDLPNP
                     MSQLVIRYHHVTVTIEDQLAVTRVDQVFFNPNDWAVEGTYIFPLPLDAVVSEFTLWVD
                     GVPVQGQVLSADEARQTYQQIVSSLQDPALLEYVGQGAVKASIFPIPPQGERRIELEY
                     TQALTADNGLVRYTYPLNTEKFSLLPLESVSVTVTIADPQSIRAVYSPSHSVDIQRPD
                     QNSARVSYEGANVLPDTDFSLYYSLGEQQAFHLFSYRTPNDPQDADGFFMLLLAPNPK
                     TIDQVIDKDILLVMDRSGSMDGEKFVQAQAAARFILENLNPGDRFYVTSFSSGIDTYA
                     SDLRPASEASSAIAWLSRQSAAGSTDINLALLDTAAVADRERPTYLIFLTDGLPTEGE
                     INSASILDNFARAAPSNLRLFAFGVGYDVDTYLLDSLSQDHHGLSTYVKPGEALDEIL
                     SAFYARISTPVLTNLQLDFGGLDVYDVYPSPLPDLFRGSQVVVVGRYRSGGTFDVTLT
                     GDVNGETMRFTYSDQVFAKDTTGLSGPLTGLPRLWATRKIGYLLNQIRLQGVDEETVD
                     QIVRLSIRYGIVTPYTSYLVTEPELLGSANQDEVVREAYNDLMAQPTQVSGEAAVNKA
                     ADQGALSAAEAPPPAVVTEEGAASAPIRIAGDHTFIQQDGVWLDTTYDPQTMTTVKVS
                     FLSAEYFQLASARPDVAAALALGDRVIVMVDGVAYEVTTDAVTGDVPLPDPVTPPVVT
                     EVVGTPIVVVPDVVDPIEDPQPQSSPSNGFCPAVLIPLGAALGFALYRRRIL"
     gene            complement(5217..5837)
                     /locus_tag="PKL11_05330"
     CDS             complement(5217..5837)
                     /locus_tag="PKL11_05330"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNZ13059.1"
                     /translation="MKTKIIATQAQQALPEALKTLQQGGLVAFPTDTVYGLAALAHRA
                     DAILQLFEAKGRDTTKAIAVLIGSLDQLDQMTPGLPKSALKLARRFWPGALTLVVERH
                     PDLPEVLSPLPTIGIRMPDHALALALLQASGPLATTSANRSGAANPLTAADVLAQLDG
                     QVDLILDGGACPGGVPSTVVDCTGPELKILRVGAITEAMIFEAILK"
     gene            complement(5834..6688)
                     /gene="prmC"
                     /locus_tag="PKL11_05335"
     CDS             complement(5834..6688)
                     /gene="prmC"
                     /locus_tag="PKL11_05335"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNZ13060.1"
                     /translation="MTTNNVGGWAAAAIQRLASETDTPGLEAQLLLGHVLGWPRTRVA
                     AHPEITLSVEEVEQLEALLSRRVNGEPLPYLLGEWEFYGLKFWVTPDVLIPRPETELL
                     VEIALGWLQTHPEKRRAADVGTGSGCIAVALTRQVPDLHMTAIDSSPAALKIATKNMQ
                     RHDVEVRINCVQSDLLNACHGPFDLVCANLPYIPSETVDGLPVAAYEPRQALDGGPDG
                     LDLIDRLLTDSPRWLAPGGLMLLEIESGQGQSAAALAQSRLPEALIVTLPDLAGKPRI
                     LRVEKVAE"
     gene            complement(6675..7751)
                     /gene="prfA"
                     /locus_tag="PKL11_05340"
     CDS             complement(6675..7751)
                     /gene="prfA"
                     /locus_tag="PKL11_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNZ13061.1"
                     /translation="MLEKIAVIEARYEELNRLLEQTGEDYQRAAELAKERAELEDVVT
                     AGREYRQTLQRLEEARALQSSEDEDLRSLAEMELEELAPRAEELGKKLKSMLVPKDPR
                     DDRNVIVEIRAGTGGDEAALFAADLFRMINRYAERRRWTVEILSQNEIGIGGYKEITF
                     AVKGRGAYSRLKYESGVHRVQRIPTTESAGRIHTSTITVAVLAEVEEVDLHIPEGDIE
                     VEVYRSAGAGGQNVQKNSTAVRITHKPTGIVVACQDERSQLQNRLRAMGILRARLYEV
                     EQEKRQQERDEDRRSQVGTGERSEKIRTYNYPQNRVTDHRVNVSSYNLPAVMDGELDL
                     FIDELSLRDETDRLSSIGEGDDDE"
     gene            7946..8728
                     /gene="pstB"
                     /locus_tag="PKL11_05345"
     CDS             7946..8728
                     /gene="pstB"
                     /locus_tag="PKL11_05345"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNZ13062.1"
                     /translation="MPEKQPDFAIEMRSVDFYYGSFRAVKQVNLAIEPRKITAIIGPS
                     GCGKSTVLRCFNRMNDLIPGTRVEGEILFRGQNIYAPDVDPVEVRRLIGMVFQKPNPF
                     PKSIYENIAWGARMNGLRGSADDLVEECLRQAALWDEVKDNLKHSALSLSGGQQQRLC
                     IARALAVHPEIILMDEPCSALDPIATLKIEDLMRELSERYTIIIVTHNMQQAARASDF
                     TAFFTMDSEDRAGLLVEYGITSQIFTNPRDSRTEDYITGRFG"
     gene            8744..9433
                     /gene="phoU"
                     /locus_tag="PKL11_05350"
     CDS             8744..9433
                     /gene="phoU"
                     /locus_tag="PKL11_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNZ13063.1"
                     /translation="MPRELMNRQLHHIQDEVLLLGSMVEQAMLGAVDALLQRNQVTAN
                     RIYRDDALINDKRFAIENAVLILIATQQPMAHDLRQLTAMLEIITELERIGDYAKGIA
                     KITHRLGDHEVPMPAHEFEQMSEIAVGMLHRALSAFINENPNEAVVISQEDDQVDSLY
                     ERAYRSIVEAMIANPATIDQSNLLLWVAHNLERTADRVTNICERTVFISTGELLEMDS
                     TDDELDTEEID"
     gene            9542..10282
                     /gene="tmk"
                     /locus_tag="PKL11_05355"
     CDS             9542..10282
                     /gene="tmk"
                     /locus_tag="PKL11_05355"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNZ13064.1"
                     /translation="MLKPNPYRGKLIVVEGIDGSGKSTQIDLLYKWLLSQGKSVYFSE
                     WNSSELVKSTTRLGKDEKAFTPTTFSLLQATDFSDRWENHILPMLKAGVIVLADRYAF
                     TAFARDVARGVDRDWVRNLYSFAPQPDLVFYFRVPVNIAVDRITTGRAKLKYYEAGMD
                     LGLSDNRVTSFRLFQQRIISEYDAMVDEFSFEVVDGADVVQDQQKRVRTAVKRLLRGY
                     EGMPNPNLPPRRSRRSALKQIATQGGES"
     gene            10279..10968
                     /locus_tag="PKL11_05360"
     CDS             10279..10968
                     /locus_tag="PKL11_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13065.1"
                     /translation="MTEPVYYGIGFPYRIIQDLPGKLIVLEGNDGVGRSTQIAMLRKW
                     LEDEGYAVSDTGLRRSTLTQAGLENAKNGHTLGSLTMSLFYATDFADRLENQIIPALK
                     AGFIVLSDRYFYSTIARDVVRGADPQWARKVYGFALKPDITYYLRADVPTLVARMVHG
                     RGFNYWEAGMDIRCADNLYDSFCVYQSRLLQEFDAMTLEYGFETVDACRNAMEIFDDL
                     RGRLRKLLAHP"
     gene            10979..11986
                     /locus_tag="PKL11_05365"
     CDS             10979..11986
                     /locus_tag="PKL11_05365"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNZ13066.1"
                     /translation="MKKAPPTDIQIYGAAALLQHLGSLLTEAPGVTTAQDIECIHRMR
                     VASRRLRAALAIFEPHLPRKRAARWQEQVRALTKALGVARDADVQIEWVRAFYQNLPA
                     GPDRPGARRLLLRLQQQRQALQPRVHRTLSRFEKSGTALSLTQTLSPPANLKTEQFAP
                     GNTIYQWAEQWIIQRLDEFLAFAPFIAQPEAQKELHAMRIAGKRLRYTLEIFAELYPD
                     ALKRPLQTLRKAQDLLGQIHDDDVWQVTLPELLAQERQRTLEYYGTLRPFNRLQPGML
                     AFAQHCHEDRTAAYAELVNAWADWENRQIWTALRQSVTTPAPEDPPHEPTSDPSPAAD
                     A"
     gene            11946..>12460
                     /locus_tag="PKL11_05370"
     CDS             11946..>12460
                     /locus_tag="PKL11_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ13067.1"
                     /translation="MNPPQTPHQPLTPEQQVAWTAALDLAQSCEVDARHAQQVTRLSL
                     RLFDLFADLHQLGSQERFWLQCAAVLHDIGWLEGWKGHHKATLQIILNTNLLPFDART
                     RLIIGSIARYHRRALPSQKHDHFATLSAAEQQMVSQLAALLRIADGLDRLHQNTIADL
                     EAQIGEKKVTLR"
     assembly_gap    12461..12560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12561..13183)
                     /locus_tag="PKL11_05375"
     CDS             complement(<12561..13183)
                     /locus_tag="PKL11_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13068.1"
                     /translation="MKFSFRQIVLLTGIGMLLCSTLGCAINRPKDGGDEIMDPQGVIE
                     KVNNVLPTGYIPEHFTQEVGSYAPQPGDFDPMAFFEALDALTIKEGYRLDYVYYFDGF
                     GGFPVLYVRSEEQAPFSSVEELDASPDAALMRDYMSYIQPDDTADGYYQYVVFSIMVQ
                     QFYLFWHSNYNDVQIVCTPEKIRQIIDATGEFGSEMPVDDKAKSLLI"
     gene            complement(13325..>14170)
                     /locus_tag="PKL11_05380"
     CDS             complement(13325..>14170)
                     /locus_tag="PKL11_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13069.1"
                     /translation="IIAGIVMLCLFGGLVLVPLFYVMSNDTVSIWYLIVPLGLFFLIL
                     TGGGVGLLFWVTAARKRTVDAAFTPWGLAGSNYLMTFRQYHGSVQGRQVDTYYYRGPI
                     LDIDIQTSLKTRMGVTGRQGDTIFLAKLAGRAPMQLPSELSDLTVFPSDEAWVRDLVN
                     DPQAAEILRRLTTLNGDFSRQQILLRPGTLRLQLSGSHKMMIFSANFTPEQVNAWMQD
                     LFALVRIMESLPAPAVEEPVSGADNVSRQIRKVNWTLVAVLIAGGVMLCAIGAGVLAY
                     ILASL"
BASE COUNT         2845 a         4421 c         3576 g         3128 t
ORIGIN      
        1 ctctttatca cccatgatat tgacctggcc gtcgtctatg ccaaccgggt aattgtggtc
       61 aacaacgggc aaatcgtccg cgacggttcg cctgtagaag tgttgaaaga tatggacatg
      121 ttggcccaga atcgtctggt gcccacctcc ctgctgcggc tcaatctgga acgatacccc
      181 cgtacgcagc atttccagcg cgccgaggat ctggcgcacc ttcaataaag caggttttct
      241 aaattccatc tcatcattca cccggaggaa ccatgaaaaa taaagctgta accgttgtat
      301 tggccctgtt ggctgttctg gccttctttg tgggcatctg gcagatcggc cgcctgatca
      361 accccagcct tgacctcgat gagaccgcgc tgctgcgctt tgtctttgtc ggcattggcc
      421 tgttgattgt ctttgtggtc tactacctga cccgccagag caaaatctgg gaagtgggca
      481 cccgcgaagt ggtctatatg gcgattggcg ctgcgctcta tgcgctcttc tcctatatct
      541 tcaacggctc ggtcttcgtt gtgccttcgg tcagccaggt ggcgctccgc ccggccattt
      601 ccatccccat gttctttggg tttgcctttg gcccggtggt cggcttcttc accggcgcgg
      661 tcggcaacat gtttggcgat gccctcaccg gcttcggcct ttcgccccag tggagcgttg
      721 gtaacggcct cgtcggtatg atcgccggcc tctggatgct cttcaaagac aagcagaaga
      781 gcatgaacat catcctgatc gtcagcgctg ttctggcgct gctggcaacg ggcatcttcc
      841 tgctcaaccg caccctcccc aacatgctgt tcttcgatgt ggacaacggc atcttcggtg
      901 atcagcagat ctccatcttc gccggcctca ccgctgtcat cggctttgcc ctggtgctgc
      961 tggtgcgctt cctgttcaaa aacaatgtgg atgtcgccgc tgccgtaacc tggagtatgc
     1021 tgggcaacct gctgggcatt ggctttgccg ccgtttccga tatctggatc aacggcttct
     1081 ctccggccgt ggccatcgtt ggcgagttca tcccctccgc cggccccaac ctgatctttg
     1141 ctgccatcct ggtcccgatc ctcgttgctg cttttgcggc tgtgcggcgc cagtcgggtc
     1201 gctagtttta gtccctttgt gattgctccg gtggtcttcc gccggagcaa tcaccccaca
     1261 ggttgaatca tgctggttac ctggcgatat cgcatccgca aatccatcat ccagttcttt
     1321 gatccgcgcg cctgggtcat ctttttcatt tgcttcctgg cctcggtgct ggctttttgg
     1381 gatttgcgct acctgctctt cttctttgtc ctgactgtcg gcatcaccct ggctgccgat
     1441 gtcagttggg cagagattcg ccgggcggtt ttgtttatcg gcgggttcat ccttttcttt
     1501 gccttcctca ccttccttac cgggcgcggg ggcatggaac tgtattcaga agaacacatc
     1561 ctgcgcgaat ttcgggctcc atttgatctc ttcggctggg ttcccacgct gaaaatcacc
     1621 gccgagcgca tcatgtatgc cgtgagcatg atggcgcgcg tgttcagcat cgccattatg
     1681 acaatcctca tcccctattc gctgaacccc gcgctctatg gggtcacctt ccgcggcctg
     1741 ggcctgcccg ataaaattgc ctacgccatg gatctgacca tgcgcttcat ccccacctat
     1801 gggcgtgatt tcaccctgac gatggatgcc cagcgagccc gcggttttga actggataag
     1861 gtcaaaggcg gcctgtttgc ccaggttcgc aagctggccc ccctggtggt gcccgtgacc
     1921 attcacgcca ttgccggcag cgaagatatt attgacgcca tggatttacg cgcctttggc
     1981 gttggcccgc gcacctggct ggacgagctc aaattccatt ggtacgactg ggttttgatt
     2041 gggctggggg ttctcatgct gctcttcagc acggcgctgg cgctctttgg ctacggcaag
     2101 ttttgggttc ccaatttctt gttacctggc tcgtagtaca atttccatac cnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggcccggt ccggtggagg gtgaacgccc
     2281 ttcggcctac atcatcgtcc tgggcgacaa agaaattgcc caaaatttca atattgatcc
     2341 cggcattgct gttcaaacta ttctcctggg cgctgtggct cagggcctgg gcggctgcat
     2401 catcgcttcg gtcaatcgca aagagctgat ccccctgctc cagattcctg accggtacga
     2461 agtcttgttc gtgctggcat tgggcaaacc cgttgaagaa gttgtcgttg accccatcgc
     2521 cccctctggc gatatcaaat actggcgcga cgaaaaacac gtccatcacg tccccaagcg
     2581 tgccctggat gatctgattg tagatttgta agcccgccag ctaaagctga atcgtctcca
     2641 atacatccct gccggaactc ctggcaggga tttgacgtct tatttctacc gttcccgttt
     2701 tttcaagccg tgtttgaacc gccagacgtc acgccgggcg tcagtttaaa cgcgtctcta
     2761 ttcaggaggt cgaaatgaaa ccgaagtcgt tgattgcctt tttgctgggc ttgctgctca
     2821 ccctggtatg ggtgatcccc gttcaagccg atggcatcgt catcccgggc tgcccgcccg
     2881 atggttgcca ggatttgccc aaccccatga gccagctcgt catccgctac catcatgtca
     2941 ccgtcaccat tgaagaccag cttgctgtca cccgggtgga tcaggttttc ttcaacccca
     3001 atgattgggc agttgaaggc acttacatct tcccgctgcc gcttgatgcc gtggtcagcg
     3061 agttcaccct ctgggtggat ggcgtgcctg tgcagggtca ggtcctctct gccgatgaag
     3121 cccgccagac ttatcagcag attgtcagca gcctgcaaga cccggccttg ctggagtacg
     3181 tcggtcaggg ggcagtcaag gccagcatct tccccatccc accccagggc gagcgccgca
     3241 ttgagctgga atatactcag gccctgactg ctgataacgg tctggtgcgc tacacctatc
     3301 ccttgaacac tgaaaaattt tccctgctgc cgctggagag cgtctcggtg accgtcacaa
     3361 ttgccgatcc gcagtccatt cgcgcggtct attccccctc acactctgtg gatattcaac
     3421 gccccgacca aaattcagcc cgggtcagct atgaaggcgc caatgttctg cccgataccg
     3481 atttctcgct ctactattcc ctgggcgaac agcaggcttt tcaccttttc agctaccgca
     3541 cccctaacga tccacaggat gccgatggct ttttcatgct gctgctggcc cccaacccca
     3601 aaaccattga ccaggtcatc gacaaagata ttctcctggt gatggatcgc tctggcagca
     3661 tggatggcga aaagtttgtc caggcccagg cggccgcccg gttcattctg gaaaacctca
     3721 accccggaga tcgtttttat gtaacctcct tcagcagcgg cattgatacc tacgcatcgg
     3781 acctgcgccc cgccagcgag gccagctctg ccattgcctg gctgagccgc cagagtgccg
     3841 ccggcagcac cgatatcaac ctggccctgc tggatactgc cgccgtggcc gatcgtgagc
     3901 gccctaccta tctcatcttc ctgaccgatg gcctgcccac tgagggtgag attaacagcg
     3961 ccagcattct ggataatttt gcccgcgcgg cccccagcaa cctgcgcctg tttgcctttg
     4021 gcgtaggcta tgatgtcgat acctacctgc tggactcgct ttctcaagat catcatggcc
     4081 tcagcaccta cgtcaaaccc ggtgaagcgc tggatgaaat cctgtcggcg ttctatgccc
     4141 gcatcagcac gcccgtgctc accaacctcc agttggattt tggcggactc gatgtctatg
     4201 atgtctaccc ctccccgctg cccgatctgt tccgcggcag ccaggtggtg gtggtcgggc
     4261 gctatcgctc cggtggcacg tttgatgtga ccctcactgg tgatgtgaac ggtgaaacca
     4321 tgcgcttcac ctattccgac caagtctttg ccaaagatac cactggcctc agtggcccgc
     4381 tcaccgggct gccgcgcctc tgggccacgc gcaagattgg ctatctcctt aatcagatcc
     4441 gcctccaggg tgtggatgaa gaaaccgtgg atcaaattgt ccgcttgagc atccgctacg
     4501 gcatcgtcac cccctacacc tcctatctgg tcaccgagcc ggaactgctg ggttcagcca
     4561 atcaagacga agtagtccgg gaagcctaca atgatttgat ggcgcagccc acccaggtga
     4621 gcggtgaggc ggcggtcaac aaggccgccg accagggtgc actctcggct gctgaagcgc
     4681 cgccacccgc agtggttacc gaagaaggcg cggcgtccgc gcccatccgc attgccggtg
     4741 accacacatt catccagcag gatggcgtct ggctcgatac cacctacgac ccccagacca
     4801 tgaccacggt gaaagtgagc ttcctctcgg cggaatactt ccagcttgcc tctgcccgcc
     4861 cggatgtcgc cgccgccctg gccctcggag accgtgtgat tgtcatggtg gatggcgttg
     4921 cttatgaggt gaccaccgat gcggtgaccg gggatgtgcc cctgccagac cccgtaaccc
     4981 cacccgtagt cacagaggtg gttggcacac ccattgtggt cgtccccgat gtggttgatc
     5041 caattgaaga cccacagccg caatcatccc catccaatgg cttttgcccg gccgtgctca
     5101 tccccctggg tgccgctttg gggtttgccc tttaccgccg ccgcatattg tagcccgctt
     5161 gattggttat aacaatcagg gcagcttctg tggctgccct gactgggttt aatgacctat
     5221 tttaaaatag cctcaaaaat cattgcttca gtaatggccc ccacccgtaa gatcttcaac
     5281 tcagggccgg tgcagtccac caccgtggaa ggcaccccgc cggggcaggc gccgccatcc
     5341 agaatcagat ccacctgccc atcgagttgt gccagtacat ctgcagcggt cagcgggttg
     5401 gctgcccccg accggttggc cgaggtggtt gccagcggtc cgctggcttg cagcagtgcc
     5461 agcgccagcg catgatccgg catgcggatg ccaatcgtcg gcagcggcga gagaacttcg
     5521 ggcaggtcgg ggtggcgctc caccaccagc gtcagcgcac ccggccagaa ccgccgggcc
     5581 agcttcaatg cggattttgg cagaccgggt gtcatctggt ccagttgatc cagactgcca
     5641 atcaacacag caatggcctt ggttgtatca cgccccttgg cttcaaataa ctgtagaatg
     5701 gcatctgccc ggtgtgccag tgccgccaat ccatacacag tatcggttgg aaaggccacc
     5761 agcccgccct gctgcagtgt tttcagcgct tctggcagcg cctgttgcgc ttgcgtcgcg
     5821 ataatttttg ttttcattcg gccacctttt ccacccgcaa aattctgggt tttccggcca
     5881 ggtcgggcag tgttacgatc agcgcttcgg gcagccggct ttgcgccagc gccgccgccg
     5941 attgtccctg cccggattca atttccagca gcatcaatcc accaggcgcc agccaacgcg
     6001 gcgagtcggt cagcagacgg tcaatcaagt ccagcccatc aggcccgcca tccagcgcct
     6061 ggcgcggttc gtaggctgcc accggcaaac catctactgt ctcactggga atgtagggca
     6121 ggttggcgca gaccaggtca aaaggcccgt ggcacgcatt cagcaagtca gactgcacac
     6181 agtttatccg cacctcaacg tcatgccgct gcatgttttt agtcgcaatc ttcagcgccg
     6241 cgggtgaaga atctatcgcg gtcatgtgta aatctggcac ctgccgggtc agcgccaccg
     6301 caatgcagcc cgaccccgtg ccaacatctg cagcccggcg cttttcagga tgggtttgca
     6361 gccatcccag tgcaatttca accagcagct cagtctcagg gcgggggatc aagacatccg
     6421 gggttaccca aaacttcaag ccataaaatt cccactcgcc cagcaggtag ggcagcggct
     6481 caccgttgac gcgacggctt aataatgcct ctagctgctc cacctcttca acagataatg
     6541 taatctcagg gtgcgccgcc acccgggtgc gcggccagcc caaaacatgc cccagcagca
     6601 actgtgcttc caatccgggt gtgtcggttt cactggctaa ccgctggata gccgcagccg
     6661 cccagccgcc gacattattc gtcgtcatcc ccttcgccaa tggaggatag ccggtcggtt
     6721 tcatctcgca gcgacagctc atcaatgaat aagtccagct cgccatccat aactgccggc
     6781 aggttgtatg acgaaacatt cacgcggtga tccgtcaccc ggttctgtgg ataattatat
     6841 gtgcggattt tctcagagcg ttcgcccgta cccacctgtg agcggcggtc ctcgtcgcgt
     6901 tcctgctgtc gcttttcttg ctctacttcg tatagccgtg cgcgtaaaat ccccatcgcc
     6961 cgcagccggt tctggagttg tgatcgttcg tcctggcagg ccaccacaat gccggttggc
     7021 ttgtgggtga tgcgcactgc cgtggagttc ttctgaacat tctgcccgcc tgcgccggca
     7081 gaacgataca cttcaacctc aatgtcgcct tctggaatgt gcaggtccac ttcttccact
     7141 tcagccagca ccgccacagt gatggtggag gtgtgaatcc gccccgcaga ctcggttgtc
     7201 ggaatccgct gcacccggtg cacccccgat tcatacttca accgtgagta cgccccacgg
     7261 cctttgacgg caaaggtgat ttccttgtat ccaccgatcc caatctcatt ctgcgagagg
     7321 atttccaccg tccagcggcg gcgctcggcg taacggttaa tcatccggaa aaggtctgcc
     7381 gcaaacagcg cagcttcgtc gccgcctgtc cctgcccgaa tttccacaat cacattgcgg
     7441 tcatcgcgcg ggtctttggg caccagcatt gattttaatt ttttgcccag ttcttcagcg
     7501 cgcggggcca gctcttccag ttccatctca gccagcgagc gcaggtcttc atcctcactg
     7561 ctttgcaaag cacgggcttc ttccaggcgt tgcagcgtct ggcgatactc gcgcccggca
     7621 gtcaccacat cttccagttc ggcgcgttct tttgccagct ctgccgcgcg ctgataatcc
     7681 tcgccggttt gttccagcag gcggtttaat tcttcgtatc gggcttcaat tactgcaatc
     7741 ttttccagca tctctctaac cttcgccgga ggagcgctcc ggatgatgtg ttttttagaa
     7801 ccgcaattat actacgaaca ataaccctct gcaccaccgc ctgctggcga atccttaact
     7861 gaaagtttac aatcccttca tcgcataaat tggttatccg gcgatataat tagtcaatcc
     7921 tgtaacggag ccaactattt tgccaatgcc agagaagcaa cccgattttg ccattgaaat
     7981 gcgatccgta gatttctact acggcagctt tcgcgctgtc aagcaagtca acctggcgat
     8041 tgagccgcgc aaaattaccg ccatcatcgg cccctcgggc tgcggtaaga gcaccgtctt
     8101 gcgctgtttc aaccgcatga atgatttgat ccccggcacc cgggtggaag gggagatctt
     8161 attccgcggg caaaatatct acgcccccga tgtcgatccc gtcgaagtcc gccgcctgat
     8221 cggcatggtc tttcagaagc ccaacccctt ccccaaaagc atttatgaaa acattgcctg
     8281 gggtgcccgc atgaacggtc tgcgtggcag cgccgatgat ctggtagaag agtgcctgcg
     8341 ccaggccgcg ctgtgggacg aggtcaaaga caacctgaaa cacagcgccc tgtccttgtc
     8401 cggcggtcag cagcagcgcc tctgcattgc ccgggcgctg gccgtccacc ccgaaatcat
     8461 cctcatggat gaaccctgtt cagccctgga tcccatcgcc accttgaaaa tcgaagacct
     8521 gatgcgcgaa ttatccgagc ggtacaccat catcatcgtg acccacaaca tgcagcaggc
     8581 cgcccgcgcc tctgatttta ccgccttctt caccatggat tctgaagatc gcgccggttt
     8641 actggtagaa tatgggatta ccagtcaaat tttcaccaat ccacgcgatt cccggactga
     8701 agattacatc accgggcgct ttggatagaa aacgagggcc aaaatgccgc gagaattgat
     8761 gaaccgtcaa ctgcatcata ttcaagacga ggtgctgctg ttgggcagca tggtcgagca
     8821 agccatgctg ggcgcggtag atgctctgct tcaacgcaat caggtgactg ccaatcgaat
     8881 ttaccgtgac gacgccctga ttaatgacaa acgttttgcc attgaaaacg ctgtcctgat
     8941 tctgatcgcc acccagcagc ctatggcgca tgacctgcgc caactcaccg ccatgctgga
     9001 gatcatcacc gaactggagc gcattggcga ctacgccaag ggcatcgcca aaatcaccca
     9061 ccgcctgggc gatcacgaag tgcccatgcc tgcccatgag tttgagcaaa tgtctgaaat
     9121 cgcagtgggc atgctgcacc gggctttaag cgccttcatt aatgagaacc ccaatgaggc
     9181 tgtagtgatc tcgcaggaag atgaccaggt ggacagcctt tacgagcggg cctatcgctc
     9241 cattgtcgaa gccatgattg ccaaccccgc caccattgac cagtccaacc tcttgctctg
     9301 ggtggcgcac aacctggagc gcaccgccga tcgtgtgacc aatatctgcg agcgcactgt
     9361 cttcatctcc accggcgaac tgctggagat ggactcaacc gacgacgaac tggatacaga
     9421 agaaatcgac taaacccctg ttcacaaaaa aagtccccga aatttttcgg ggactttttt
     9481 gaaagtgcta taattatttc atatttggta acgcccgcct ggcgggaaag gatcgaccat
     9541 cgtgctaaaa cctaatccct atcgcggcaa attgattgtc gttgaaggca tcgacggcag
     9601 cggcaaatcc acgcaaattg atttgctcta taagtggctc ctgtctcagg gtaaatcggt
     9661 ctattttagc gaatggaact cctccgaact ggtcaaaagc accacccggc tcggcaaaga
     9721 cgaaaaagcc ttcaccccca caaccttcag cctcctgcag gccaccgatt tttctgaccg
     9781 ctgggaaaat cacatcctcc ccatgctcaa agcgggggtc atcgtcctgg cagatcgcta
     9841 cgcctttacc gccttcgccc gggatgtagc ccgcggggtt gatcgagact gggtccgcaa
     9901 cctgtattct tttgcccctc aacccgacct ggtcttttac ttccgtgtcc cggtcaatat
     9961 tgctgtagat cgcatcacca ccggccgcgc caagctcaaa tattatgaag ccggcatgga
    10021 cctgggcctg tcagataatc gcgtcaccag cttccgcctc ttccagcagc gcatcatcag
    10081 cgagtatgat gccatggtgg atgaatttag tttcgaggta gtggatggcg ccgatgtggt
    10141 tcaagatcag caaaagcggg ttcgcacagc ggtcaaacga ctgctgcgcg gctacgaagg
    10201 catgcccaac cccaacctgc ccccccgccg ctcccggcgc agcgcgctca aacaaattgc
    10261 cacccaggga ggtgaatcat gaccgagccc gtctattacg gcattggctt tccctatcgc
    10321 atcattcaag acctccccgg caaattgatt gtgcttgaag gcaacgatgg ggtgggacga
    10381 tccacccaga ttgccatgct gcgcaaatgg ctcgaagatg aaggctatgc agtttccgat
    10441 accggattgc gccgctccac gctcacccag gctggcctgg aaaatgccaa aaacggtcat
    10501 accctcggca gcctgaccat gagcctgttc tacgccaccg attttgccga ccggctggaa
    10561 aatcaaatca tcccggcact caaagcgggg tttatcgtcc tctcagatcg ctatttctac
    10621 tcaaccatcg cccgtgatgt cgtgcgcggc gccgaccctc agtgggcgcg caaggtctat
    10681 ggctttgccc tcaagcccga tatcacctac tacctgcggg cagatgtgcc cacgcttgtg
    10741 gctcgcatgg tgcatggtcg cggcttcaac tactgggaag ccggcatgga tattcgctgc
    10801 gccgacaacc tctatgacag tttttgcgtt tatcaatccc gcctgctgca agagttcgac
    10861 gccatgaccc tcgagtatgg ctttgaaacg gtggacgcct gccgcaacgc catggaaatt
    10921 ttcgacgatc tgcgcggccg tctgcgtaaa ctgctcgccc acccctgatg cgccgcacat
    10981 gaaaaaagcg ccgcccaccg atatccagat ttacggtgcc gccgccttgc tgcagcacct
    11041 cggcagcctc ttgaccgaag cgccgggcgt gactaccgcc caggatattg agtgtattca
    11101 ccggatgcgg gtagcctcgc gccggttgcg cgccgccctg gcaatttttg agccccacct
    11161 gccgcgcaag cgcgccgcac gctggcaaga acaggtccgc gccctgacca aagctctggg
    11221 cgtcgcccgt gatgccgatg tccagattga atgggtgcgc gccttttacc agaacctgcc
    11281 tgccggccca gaccgccccg gcgcgcgccg cctgctgctc cggctgcagc agcagcgcca
    11341 ggccctgcag cccagggtgc accgcaccct ctcccggttc gaaaagagcg gcacagccct
    11401 gagcctgacg caaactctct cccctcctgc caacctgaag actgagcagt ttgcgcccgg
    11461 gaacacaatc tatcaatggg cagaacaatg gattatccaa cgcctggatg aattcttagc
    11521 ctttgcgcct ttcattgctc aacccgaagc gcagaaagaa ctccatgcca tgcggattgc
    11581 tggcaagcgc ctgcgctaca ccctggaaat ttttgccgaa ctgtaccccg acgccctcaa
    11641 gcgcccgctt caaaccctcc gcaaagccca ggacctgctt gggcagatcc acgatgacga
    11701 tgtctggcag gtgaccctgc cagaactgct ggcccaggaa cgtcagcgca ccctggaata
    11761 ttacggcacc ctgcgcccct tcaaccgcct tcagccgggc atgctggcct ttgctcaaca
    11821 ctgccacgaa gatcgtaccg ccgcctatgc cgaactggtg aatgcctggg ctgattggga
    11881 gaaccgtcaa atttggacag ctttgcgaca gtctgtgacc accccggccc cggaggatcc
    11941 cccgcatgaa cccacctcag acccctcacc agccgctgac gcctgaacaa caggtcgcct
    12001 ggaccgccgc gctagacctg gcgcagtctt gcgaagtgga tgcccgccat gcccagcagg
    12061 tcacccgcct gtccttgcgc ctgtttgacc tgttcgccga tctgcaccag cttggcagcc
    12121 aggaacgctt ctggctgcag tgcgccgccg tcttgcatga tatcggctgg ctggagggct
    12181 ggaaaggcca ccataaagcc acgctccaaa tcatcctcaa caccaatctg ctgccctttg
    12241 acgcccgcac gcggctgatc atcggctcca ttgcccgcta ccaccgccgt gccttgccct
    12301 cgcagaaaca cgaccatttt gccaccctct ctgctgccga gcagcaaatg gtcagccagc
    12361 ttgctgcgct gctgcgcatt gccgacggcc tggaccgctt gcatcaaaac acaatcgccg
    12421 acctggaagc ccagatcggc gaaaagaaag tcaccctccg nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn ttaatcagca gtgatttcgc cttatcgtcc acgggcattt
    12601 ctgatccgaa ctcacccgtt gcatcaataa tctgccggat cttttccggt gtgcagacaa
    12661 tctgcacatc gttgtaattg gaatgccaga acagatagaa ttgctgcacc atgattgaaa
    12721 agaccacata ttgataataa ccatcagcgg tatcatcggg ctggatatag ctcatataat
    12781 cccgcatcaa cgctgcatcg ggactggcat ccagttcctc aacactactg aagggcgcct
    12841 gttcttctga acgaacatac aaaaccggga acccgccaaa gccatcaaaa taatagacat
    12901 aatccagtcg gtagccttcc ttgatcgtca gggcatccag agcctcgaaa aaggccatgg
    12961 ggtcaaaatc gccgggctgt ggcgcgtaac tgccaacttc ttgcgtaaaa tgttctggaa
    13021 tatagccggt gggaaggaca ttattgacct tctcaatcac accctgcgga tccatgatct
    13081 catctcctcc atcttttggc cggttgatgg cgcagcccaa tgtagagcac agcagcatcc
    13141 cgatgccggt taataaaaca atctggcgaa aactaaactt catctttatc tccttgtttg
    13201 actgaccttt gaatgggact ggcagacgta gacttctggc gtacagccga gataaagacg
    13261 cctggcgcag ccgggaagtt cccagccaca gcccgtaaaa cgcaaattgt ctggatcagt
    13321 tcccctaaag actggccaga atataagcca gcaccccggc cccaatggca cacaacatca
    13381 cgccgccggc aatcagcaca gccaccagcg tccagttcac cttgcggatt tggcgcgaaa
    13441 cgttatccgc tccgctgacc ggctcctcaa ccgccggggc tggcaggctc tccatgatgc
    13501 gcaccagtgc aaacaggtcc tgcatccagg cgttcacctg ctcaggggta aagttcgcgc
    13561 tgaaaatcat catcttgtga ctgcccgaaa gctgcagccg cagcgtccca gggcgcagca
    13621 gaatctgctg ccggctgaaa tcgccgttca gcgtcgtcag ccgccgcaaa atttccgctg
    13681 cctgcgggtc attgaccagg tcacgcaccc aggcttcgtc acttggaaag accgtcaaat
    13741 cgctcaactc tgacggtaac tgcattggag cgcgtccggc cagttttgcc agaaaaattg
    13801 tgtctccctg ccgcccggtg acgcccatcc gggttttgag actggtctga atgtcaatgt
    13861 ccaggatggg accccggtag taataggtat ccacttgccg cccctgcact gagccatgat
    13921 actgccggaa cgtcatcaga taattgctgc cagccagccc ccagggggta aaggcggcat
    13981 ccaccgttcg cttgcgcgcc gccgttaccc agaacagcag accaaccccg cctccggtca
    14041 aaatgaggaa aaacaggccc agcggaacaa tcagatacca gatgctaacg gtatcattac
    14101 tcatcacata aaacagcgga accaacacca gcccgccaaa caggcagagc atcacaatcc
    14161 cggcaataat
//
LOCUS       DAOPVS010000043        14134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4401_length_14134_cov_56.865118, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000043 DAOPVS010000000
VERSION     DAOPVS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14134
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4401_length_14134_cov_56.865118"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PKL11_05385"
     CDS             complement(<1..905)
                     /locus_tag="PKL11_05385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13070.1"
                     /translation="MSLTCHPYAAPHDLDVLLAFLRQIRPPERAADFPSAANLRELLS
                     LTAVQANAALWRNAAGQLAAYALVDHYNNLIFELDPQLASPDLPGEIIAWGVARRRSR
                     LTEEDELPTLDASCSADNLEQIALLEAHGFVREPITSVHLARSLSEPIPQPVLPPGFT
                     IRPTGGAAEAEAWVVLHRAAFGTEHMELEERLAMLAEPEYDPQVDLMAVAPDGRLAAY
                     CMVQISRSENARTGRSEGYTDPVATHPDFQRRGLARALLLTGLHLLKDRGIATALMGT
                     SSENAPMLATARSVGFVVVSTSVWFS"
     gene            complement(921..1622)
                     /locus_tag="PKL11_05390"
     CDS             complement(921..1622)
                     /locus_tag="PKL11_05390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ13071.1"
                     /translation="MKRALIVGAALAIAVGAGFVYFYWLAPGRSAQRSRQVIAYIRNP
                     EDHADWVIPAGARCGQAPFQMPTTGFIGYLWGDSFRIGHQHSGLDIFGGTQPGQTPVY
                     AAYDGYLTRLSDWKSTVIIRIPSDPLLPGRQIWTYYTHLADPDGNSLVDAAFPPGTSE
                     VFVPAGTLLGYQGNYSGTPGSPTGVHLHISIVLDDGAGHFRNEMDIANTLDPSPYFGL
                     PLNGRTNTDTIPLCP"
     gene            complement(1619..>2521)
                     /locus_tag="PKL11_05395"
     CDS             complement(1619..>2521)
                     /locus_tag="PKL11_05395"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNZ13072.1"
                     /translation="KTQRGKQVTAFVPVVLGCSHACTYCIIPYRRGIEVSRPPEQILS
                     EARALVEQGVREITLLGQIVDRYGKDEPSYPTLAALMRQLQALDGLERIRFLTSHPNW
                     MTDELIDCVAQEPKIMPHIEVPIQAGDDAVLAAMKRGYTADDYRRLVARLRERIPGVS
                     IATDIIVGFPGETDAQFQQTYDLLAELRMDVAHLARYSSRPGTVATRNLPDDVPAAEK
                     LRRFRALEVLQEEIATEIHARLLGQTVPVLFEDKSKARWRGRTPTNKLVFVETNTDLR
                     GQMLPVKITWTGPWSMIGELAEAA"
     assembly_gap    2522..2621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2622..3078)
                     /locus_tag="PKL11_05400"
     CDS             complement(<2622..3078)
                     /locus_tag="PKL11_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNZ13073.1"
                     /translation="MNVADSVRVASALEHLGYQQTGRPDEADVIVLNTCVVRQSAEDK
                     AYGRLMSLRPLKESNPNLVINLMGCLVGVRAPRELQKRFPFVDVFSPPSDPAPLVAYL
                     TQSQDRATEQAETQARFDWMDELDSFGYLRLPKTQRGKQVTAFVPVVLGC"
     gene            complement(3442..4419)
                     /locus_tag="PKL11_05405"
     CDS             complement(3442..4419)
                     /locus_tag="PKL11_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNZ13074.1"
                     /translation="MYPAKLSPGDEVRIVAPATSFSHITAATRQNATAQLAALGLRVT
                     YAEHAEEKDSFNSSSVASRVADLQAAFADPNVKAILTAIGGFSSNQLLRALDYDLIRA
                     NPKILCGYSDITALASAIYAKTGLVTYSGPHFSTFGMLRGLEYTLEYFRKCLFEPGPF
                     AVLPSPAWSDDPWYRDQENRVFVPNPGCRVINPGRAEGRLLGGNLCTLNLLQGTEYMP
                     ALAGSLLLLEDDYESKPQHFDRDLQSLIHQPGFEGVRGLVIGRFQNESEISPEKLAAI
                     IHSKPELAHLPVVAGADFGHTTPSITFPIGGTGSLTALNGAVSFTITEH"
     gene            complement(4423..5223)
                     /locus_tag="PKL11_05410"
     CDS             complement(4423..5223)
                     /locus_tag="PKL11_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ13075.1"
                     /translation="MELTPHTLPRDGFNLHYWTGGQPDAPLVVFTHGATIDHHEWDAT
                     LPLVGAHFRILTWDVRAHGLSRPAPFSLAQAENDLLTLLDTLQTDQAIFVGHSMGGNL
                     HQELVFHHPERVRAMAFLDCTWNFQKLTALEQFGLSLAEPIFRLYSYKALVDQSLAAT
                     AISPESQNLLRQSMGSLTKAEFIQIMMATSACLHEEPGYQINKPLLLMVGDQDKTGNI
                     RQAMPRWAAQEPDCRLVVIPNARHAPNLDNPSVFHQTLLDFLLTQKER"
     gene            complement(5242..5748)
                     /locus_tag="PKL11_05415"
     CDS             complement(5242..5748)
                     /locus_tag="PKL11_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13076.1"
                     /translation="MDKCVHLSPIGRLVHTEWRRLALRFKFVALDEFVIMPDHVHGIL
                     WLQAPGEAQPHQPQLSMACLALTGSVAPSDCKGEARQPDANSHHTGLARPRGAPAHSL
                     GAIVGAFKSSVTLRANLMQNASRSSVWQRNYYEHVIRTEAELNRTRLYIQQNPLKWVL
                     NRSNNLDV"
     gene            complement(5949..6371)
                     /locus_tag="PKL11_05420"
     CDS             complement(5949..6371)
                     /locus_tag="PKL11_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ13077.1"
                     /translation="MTLGAMLSDIVRSLFKKPITERYPFERKPTPERLRGKLIYDPTR
                     CTGCMLCMKDCTANAIEILTVDKVNKKFVMRFHVDRCTYCAQCVENCRFECINMSSEE
                     YEMAALTRAPFEVMYGREEDIQALIDCAEKAPTEEPAA"
     gene            complement(6374..7345)
                     /locus_tag="PKL11_05425"
     CDS             complement(6374..7345)
                     /locus_tag="PKL11_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNZ13078.1"
                     /translation="MELSLHPIILLLFFPGGLFLFVAGLLYEWVDRKLVAQYQNRIGP
                     RFIQPLADIIKLLAKEEIVPDGVHKGLFIALPIVGLASALAAALYVPLVGFNPAYSFK
                     GDLIVVVYLLSVMTLCLGLAGANTADRFSLVGATRTLTQLFSYEAPFMLSLLGPAIVA
                     GTWQISEINAFAIQSRPWWFAFTQIVGFLVAMIGLMGKLEMPPFDAPEAETEIVAGAL
                     TEYSGRGLALFRIGKDVELVIGLTLIAAFFMGGLANPLDFLWKTAILILVVAALQSLF
                     ARLRIDQTVGLWWRAGALLALLQLLAIILGRYLQPVLSQFFSGLFGG"
     gene            complement(7361..8461)
                     /locus_tag="PKL11_05430"
     CDS             complement(7361..8461)
                     /locus_tag="PKL11_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNZ13079.1"
                     /translation="MENPESPQKFVVPIGPQHPALKEPAHFEFTVDGEIVTGASVRLG
                     YVHRGIEKATEARNYPQGLYLVERVCGICSHVHALSFALGTEKLAKVVAPPRAQAIRV
                     LTSELERLHSHMLWLGVAAHEGGFDTLFMYSWRDRETVMDLLEQMTGNRVNYSINIIG
                     GVKQDVNDEIKTAILKGMDFLEGRMAHYLTVVTTDETFLGRTRDVGVMTREQAETLAP
                     VGPTARASGVPIDVRVDSPYSGYVDFPVNLIVDEGCDLLARFVVRIKEMQESIRLVRE
                     ILDHLPEGELAVRVPRRIPENEIIQRVEAPRGELFYYMKSNGTDMPERVKIRTPSLCN
                     WASVINLAPGYKLADMPMILAGIDPCFSCNDR"
     gene            complement(8474..8950)
                     /locus_tag="PKL11_05435"
     CDS             complement(8474..8950)
                     /locus_tag="PKL11_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNZ13080.1"
                     /translation="MDLETALQSAEALLQPLAVEIKRPEIERVDVYIALDNLKPAVQA
                     LVDAHWGYLAAITGVDKPAPAAEGAATTGDVEVLYTFANGGALATLRVRVPYTDAKVP
                     TICDIIPAATLFERELIEMLGVDCLGTPDPGHLILPDEWPADVYPLRKSFTGLNQE"
     gene            complement(8963..9454)
                     /locus_tag="PKL11_05440"
     CDS             complement(8963..9454)
                     /locus_tag="PKL11_05440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNZ13081.1"
                     /translation="MTELKLDTPWQRALEQIKTWARVNSPWAIHYNSGSCNGCDIEIL
                     ATLTPRYDLERFGIKLQGSPRHADILICTGPVSLQSRDRLRRIYEQMPEPKFVVAVGS
                     CGISGGAFQGCYNVVGSIGEVIPVDAYVPGCPPRPEAIIYGVVALLQSLQKKAPAPVQ
                     PAE"
     gene            complement(9509..9823)
                     /locus_tag="PKL11_05445"
     CDS             complement(9509..9823)
                     /locus_tag="PKL11_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13082.1"
                     /translation="MDIILTPPLAFLIYIPLVLIIFWIGKGLAGPEKPGSPLKRSLYG
                     SGELAPTQIAAPGYRPFFLIAFFFAILHLGVLVLATGTLDWSMVPYLVGLGLALVALL
                     LG"
     gene            complement(9836..11362)
                     /locus_tag="PKL11_05450"
     CDS             complement(9836..11362)
                     /locus_tag="PKL11_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNZ13083.1"
                     /translation="MNPVFAILWLIGLPLLASPVIYLIGRSVARRDGESSPRNPARWV
                     ALITTAAMGVPLFVLAQNLLTSGQGVVGLLFAEIPLRFDGISLVLAISVWLLGLMVML
                     YSGPYMAREVGEEKYYALLSAMMAAMIGLGTAADLFNLWVWFETMAITSFMLVAFYRD
                     EPASLEAGFKYLVQSAVGSALVLLGIGLIFMGAGTLNLQQLALMGAGAAGLPIITVAV
                     ALMIIGFGVKTALVPMHTWLPDAHSQAPSGISAMLSGVVIAAGLVALLRSMGVMAYNG
                     MAWGPIMLGFGVLNMVVGNLMALRQTVVKRLLAYSSLAHVGYMLVGIGATATFLSDQG
                     GSGAFFHLFTHAMMKGLAFLSAGALLYSLHIAKGDHDPLTVADLNGAAKRYPLIAMGL
                     SIAVLGLGGLPPLAGFMSKWQIFVGAAGSNNWWMLALVGFAALNSVLSLGYYAPLVNR
                     MVRNEPSEAVAAGKRVPALMTVPVVILSLVVLVFGFWPALFNGIGNLAGQALLRIIVP
                     "
     gene            complement(11366..13117)
                     /locus_tag="PKL11_05455"
     CDS             complement(11366..13117)
                     /locus_tag="PKL11_05455"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNZ13084.1"
                     /translation="MSTPAILILLTLGLPWLGALVVWAAGDRTEKLSHTLAVIFAVLS
                     AVAGLGLLLQTGSEPVKLADLGGVFGPISFVPDGLGVFLSVVATFIGSLAVIFSVNYM
                     HGEKQLSRYYAYMLFFIGAMVGLVLTSNMLLMFVFWEITALCSYALISFNSDDPKAVA
                     GGVKALIMTQIGGIGLLLGALVLYIYTGQMDYNILLANPAAIPAQLLPVMAFGFLVAA
                     AAKSAQFPFQTWLPDAMEAPTPISALIHAATMVNAGVYLLARFYPAFHEVPGWTFSVM
                     LVGLITALLAALMAVVANDLKRVLAYSTVSQLGYMVYAVGSGGIFASQFHLLSHSVFK
                     ALLFLAAGAVIHAVGTRDMRQMGGLGKQMPFTRVVFIIGALALAGIPILNGFWSKELV
                     LEAGLMHAPIWSYVLMLIGAGLTAFYTYRMVTMVFYGEAHGHAHGDAGTAMKIALAPL
                     ALGALSTWLLAGPFAKLLSAETLPYHGIEAASTFHILEEVISLPTLIALAVVALGLLA
                     WVFRSKLAGLGRALRGLGWAAENSFGFEAINRGIVRGVSGAAEGLRITQTGLLNWNVV
                     GIVAGLLIVIAILALGA"
     gene            complement(13122..13433)
                     /locus_tag="PKL11_05460"
     CDS             complement(13122..13433)
                     /locus_tag="PKL11_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HNZ13085.1"
                     /translation="MSNYIPHIILGVAILLLGVGIYALLATRNLIKVIVALQIMVKGA
                     MLVLVLAGKMTAQPGLGQTLALTVIVADTIVAVIGLTLAVQIRRRFGSLDVKDLTRLK
                     R"
     gene            complement(13430..13948)
                     /locus_tag="PKL11_05465"
     CDS             complement(13430..13948)
                     /locus_tag="PKL11_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNZ13086.1"
                     /translation="MEIIWYVLAAAGALVCAALAISAKRLLVSAIWLAGCSALVALLM
                     YLLGAPEIAVIELSVGAGLVTVLFVFAINIAGDESTALPVALPKPIPWILAIGLLALV
                     GWMVLSKLMGGEMPPDYGETFYATLWTSRYLDVLLQVAIIFTGVLGMMGLLAEGKKRP
                     AAEAPGSDEEHS"
BASE COUNT         3004 a         3953 c         4556 g         2521 t
ORIGIN      
        1 gaaaaccaca cgctggtgga tacaaccaca aaccccacag agcgggcggt tgccagcatg
       61 ggggcgttct cgctgctggt gcccatcagg gcggtggcaa tgccccggtc tttgagcagg
      121 tgcagtccgg tgagcagcag ggcacgggcc aggccgcggc gctgaaaatc ggggtgggtg
      181 gcaacggggt cagtataacc ctcactgcgc cccgtgcggg cattctccga gcggctgatt
      241 tgcaccatgc agtaagccgc cagacggcca tcgggcgcca cagccatcag atccacctgc
      301 ggatcgtatt ccggttcagc cagcatggcc aggcgctctt ccagttccat gtgttcggtg
      361 ccgaaggcgg cgcggtgcag cacgacccag gcctcggctt cggcagcgcc cccggttgga
      421 cggatggtga aacccggtgg taaaaccggc tgggggatgg gttcagacag ggagcgggcc
      481 aggtgcaccg aggtgatggg ctcgcgcaca aagccgtggg cttcgagcag ggcaatctgt
      541 tccagattat cggctgaaca actggcatcc agggtgggta actcatcttc ttctgtaaga
      601 cgagagcggc ggcgggccac cccccaggca atgatctcgc ccggcaggtc cggggaggca
      661 agctgcgggt ccagttcaaa aatcagattg ttgtaatgat ccaccagagc ataggccgca
      721 agctgtccgg ccgcgttacg ccacagggcc gcattagcct gtacagcggt tagtgagagc
      781 agttcgcgca ggttggcggc cgaggggaaa tcggcagcgc gctccggcgg gcggatctgg
      841 cgtaaaaatg ccagcagcac gtccagatcg tgcggagcag cgtaggggtg acaggtcagg
      901 gacataagag gctccattta ttaagggcaa agggggattg tgtcggtatt cgttcggcca
      961 ttgagcggca ggccaaagta aggcgagggg tcgagggtgt tggcaatatc catttcgttg
     1021 cgaaagtgcc cggcgccatc atccagcacg atggagatgt gcaggtgcac gcccgtaggg
     1081 ctgcccggtg tgcccgaata gttgccctgg taacccagca gcgtgcccgc aggcacaaag
     1141 acctcgctgg ttccgggcgg aaaggcagca tcaaccagcg aattgccatc agggtcggcc
     1201 aggtgggtgt aataagtcca gatctggcgg cccggcagca gcgggtcaga ggggatgcgg
     1261 atgatgacgg tggacttcca gtctgagagc cgggtcaggt agccatcgta ggcggcatag
     1321 acgggcgttt gaccgggctg tgtgccgcca aagatatcca ggccgctgtg ctggtggcca
     1381 atgcggaacg aatcgcccca caggtagccg atgaagccgg tggtgggcat ctgaaagggg
     1441 gcctggccac agcgggcgcc cgccgggatg acccaatcgg cgtgatcctc cgggttacgg
     1501 atatacgcaa tcacctggcg ggagcgctgg gcgctgcgcc ccggcgccag ccagtagaag
     1561 tagacaaacc cagcgccaac ggctattgcc agcgcggcgc cgacgatcag tgcgcgtttc
     1621 atgcggcctc cgccagctca ccaatcatag accagggacc cgtccaggtg atttttacgg
     1681 gcagcatctg gccgcgcagg tcagtgttgg tctcaacgaa aaccagcttg ttggtgggtg
     1741 tgcggccgcg ccagcgggcc ttggatttat cttcaaacag caccggcacc gtctgaccca
     1801 gcaggcgggc gtggatttcg gtggcaattt cctcctgcag cacttccagg gcgcggaagc
     1861 ggcgtagttt ttcggcggcg ggcacatcgt ccggcaggtt gcgggtggct acggtgcccg
     1921 ggcgcgatga gtagcgcgcc aggtgggcca catccatcct cagttccgcc agcagatcgt
     1981 aggtctgctg gaactgggcg tcggtctcgc cggggaagcc gacgatgatg tcggtagcga
     2041 tggagacgcc agggatgcgc tcacgcaggc gggccaccag gcggcggtaa tcgtcggcgg
     2101 tgtagccgcg cttcatcgca gccagcacgg catcatcacc cgcctggatg gggacttcaa
     2161 tgtgcggcat gatcttgggc tcctgcgcca cacagtcaat cagttcgtcg gtcatccagt
     2221 tggggtgcga ggtcaggaag cggatgcgct ccagcccgtc cagcgcttga agctggcgca
     2281 tcagcgcggc cagggtggga taggacggtt catctttgcc gtagcgatcc acaatctgac
     2341 cgagcagggt gatctcacgc acgccctgct ccaccagcgc ccgcgcctcg gacagaatct
     2401 gctccggcgg gcggctgact tcaatgccgc ggcggtaggg gatgatgcag taggtgcagg
     2461 catgtgaaca gcccagcacc accggcacaa aagccgtcac ctgtttgccg cgctgggttt
     2521 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacagccca gcaccaccgg
     2641 cacaaaagcc gtcacctgtt tgccgcgctg ggttttaggc aggcgcaggt agccaaacga
     2701 gtccagctcg tccatccagt caaagcgggc ctgggtttcg gcctgctcag tggcgcggtc
     2761 ttgcgactgc gtcaggtagg cgaccagcgg ggcggggtcg gagggagggg aaaagacatc
     2821 cacaaagggg aagcgttttt gcagttcgcg cggggcgcgc acgcccacca ggcagcccat
     2881 caggttgata accaggttgg ggttgctctc tttcagcggg cgcagcgaca tcagacggcc
     2941 gtaggcctta tcttcggcgc tctggcgcac cacacaggta ttcaacacga tgacatcagc
     3001 ctcatcaggg cggccggtct gctggtagcc caaatgctcc agcgccgaag ccacgcgcac
     3061 cgaatcagcc acgttcatct gacagccttc agtccagata tgatatttca tgcgccggat
     3121 tataacagcg ttttaggttt caagtttcaa gttttgggcg caagacaaca agatgtgtct
     3181 atgtatagga ttaacgttac tactcaggct agatgagatg ctcatttggc tgaaagtatt
     3241 tcgcccatct gcggagtgcg ggccgcgcag cgcactggaa gtgctaaagc cggcactcca
     3301 gaagaaggcc aaatccgctc atagctgagt agttaccatt ccagaagagg atcgaattcg
     3361 cttattgttg aatagttacg gattaacttt acttctgata ttgttcggtt aatcagcaac
     3421 cgctcaggtc aggcacaact atcagtgttc tgtaatcgta aagctcacag cgccgttcag
     3481 ggcggtcagc gagccggtgc cgccgatggg gaaggtgatg ctcggcgtgg tgtggccaaa
     3541 atctgccccg gcgaccaccg gcaggtgcgc cagttcgggt ttgctgtgga tgatggccgc
     3601 cagcttttcc ggcgaaattt ccgattcgtt ctggaagcgc ccaatcacca ggccgcgcac
     3661 gccttcaaat ccgggctggt ggatcagcga ctgcaggtcg cggtcaaagt gctggggttt
     3721 ggactcatag tcatcttcca gcagcaaaag gctgcccgcc agcgcgggca tgtattcggt
     3781 gccctgcagc aggttgagcg tgcacaggtt gccgcccagc agccgccctt cggcgcggcc
     3841 ggggttaatc acccggcagc cggggttggg gacaaagacc cggttctcct ggtcacggta
     3901 ccaggggtcg tcactccagg cgggggaggg cagcacggca aaggggcccg gctcaaagag
     3961 gcatttgcgg aagtattcga gggtgtattc cagcccgcgc agcatgccaa aggtggaaaa
     4021 atgcgggcca gaataggtga ccagcccggt tttggcatag atggccgagg ccagggcggt
     4081 gatatctgaa tagccgcaca aaatcttggg gttggcccgg atgaggtcgt aatccagggc
     4141 gcgcagcagt tgattgctgc tgaagccgcc aatggcggtc agaatggctt tgacgttcgg
     4201 gtcggcaaag gcggcctgca ggtcggccac ccgggaagca acggaagatg agttgaagct
     4261 gtccttttct tcggcatgtt cggcataagt cacgcgcagg cccagcgcgg caagctgtgc
     4321 agtggcgttt tggcgtgtgg cggctgtgat gtggctgaac gaggtggccg gggccacaat
     4381 ccggacttca tcaccgggtg agagtttggc cgggtacatg ggttagcgtt ccttttgggt
     4441 cagtaaaaag tccagcaggg tttgatggaa gacactgggg ttgtccaggt tgggagcatg
     4501 gcgggcgttg gggatgacca ccagccggca gtctggctcc tgggcggccc agcggggcat
     4561 ggcttggcgg atgttgccgg ttttatcctg gtctccgacc atgagcagca ggggcttgtt
     4621 gatctgatag ccgggctctt cgtgcaggca ggccgaggtg gccatcataa tctgaataaa
     4681 ttcggctttg gtcaggctgc ccatggattg gcgcagcaag ttctgcgatt cgggcgagat
     4741 ggcggtggct gccagcgatt gatccaccag cgccttgtat gaatagagcc ggaagattgg
     4801 ttcagcaagg ctgaggccaa actgttcaag ggcggtcagt ttttggaagt tccaggtaca
     4861 gtcgaggaag gccattgccc ggacgcgctc gggatgatgg aagaccagtt cctggtgcag
     4921 gttgccgccc atcgagtgac cgacaaagat ggcctgatcc gtttgcaggg tatcgagcag
     4981 ggtcagcagg tcattctcag cctgagcgag tgagaagggc gccgggcgtg agaggccgtg
     5041 ggcgcgcaca tcccaggtga ggatgcggaa gtgagcgccc acgaggggca gggtggcgtc
     5101 ccattcgtgg tggtcaatgg tggcgccatg ggtgaagacg accagcgggg catcgggctg
     5161 cccgcctgtc cagtagtgga gattgaagcc gtcgcgcggc aaggtgtgag gggtaagttc
     5221 catgggggga gcctttcaaa cctaaacatc aagattattt gaacgattaa gcacccattt
     5281 tagcgggttt tgctggatgt ataaacgagt ccggtttaat tccgcttcgg tgcgaataac
     5341 atgctcataa tagtttcgtt gccatacaga tgatctggat gcattttgca tcaaattagc
     5401 gcggagcgtt accgaggact taaaagcgcc aacaattgca cctaatgaat gcgcaggggc
     5461 gcctctgggc cgggcgaggc cggtatggtg agagtttgcg tcgggttgtc tcgcctcgcc
     5521 cttacaatca gatggagcca ccgaacccgt aagggcgagg caagccattg ataactgtgg
     5581 ttggtgcggc tgcgcctcgc ccggtgcttg cagccataaa attccatgga catgatctgg
     5641 catgatgaca aattcatcca atgccacaaa tttaaaacga agcgccaatc gccgccactc
     5701 agtgtggacc aatctgccaa ttgggctcaa atgaacacac ttatccaata cctctccaag
     5761 gattggggcg cgctgccagg tcaacaaagt tacaaaataa gcgccgggtt gggtgtaatc
     5821 atatcctggt gcacgcgggg aacaacgatt agatgacatg ttccgattat acatcttccc
     5881 ccttaaaacc agccggcggc tctggggaga gccgccggtg aagggcgata aaatcgatgc
     5941 ggggccggtc aggcagcggg ttcttcggtg ggggcttttt cggcgcagtc tatcagggcc
     6001 tgaatgtctt cctcgcggcc gtacattacc tcgaaggggg cgcgggtgag ggcggccatt
     6061 tcgtattctt ccgatgacat gttgatgcac tcaaagcggc agttttcaac acactgggcg
     6121 cagtaggtgc agcggtcaac gtggaagcgc atgacgaact ttttgttcac cttatctacg
     6181 gtgagaatct cgatggcgtt ggcggtgcag tccttcatac ataacataca gccggtgcag
     6241 cgcgtcggat cgtagattag tttgccgcgc aagcgctccg gggtgggctt gcgttcaaac
     6301 gggtagcgct cggtgatggg ctttttgaac agcgagcgga cgatgtcgct cagcatggct
     6361 ccaagtgtca tggctaacct ccaaacaaac cactaaagaa ctgggacaac acaggctgca
     6421 aatagcggcc caggatgatg gccagtagtt ggagcagggc cagcagggcg cccgcgcgcc
     6481 accacaaccc caccgtctga tcgatgcgca ggcgggcaaa cagcgattgc agcgccgcca
     6541 ccaccaggat caggatggct gtcttccaga ggaagtccag cgggttggcc aggccgccca
     6601 tgaagaaggc cgcgatgagc gtgaggccga tgaccagttc cacatctttg ccgatgcgga
     6661 agagcgccag gccgcggccc gaatattcgg tgagggcgcc ggctacaatt tcggtctcgg
     6721 cttcaggggc atcgaagggc ggcatttcca gcttgcccat caggccaatc atggcgacga
     6781 ggaagccgac aatctgagta aaggcaaacc accacgggcg gctctgaatg gcaaacgcgt
     6841 tgatctcgct gatctgccag gtgccagcca caatggccgg gcccagcagc gagagcatga
     6901 agggcgcttc gtaagagaag agctgcgtga gggtacgggt ggcgccgacc agcgagaagc
     6961 ggtcggccgt gttggcgccc gccaggccca ggcagagggt catcacgctg agcaggtaca
     7021 ccaccacgat caggtcgcct ttgaaagagt aggccgggtt gaagcccacc agcggcacat
     7081 acagcgctgc cgccagggcc gaggccaacc caacaatcgg cagggcaatg aagaggccct
     7141 tgtgcacgcc gtcgggcaca atctcttcct tggccagcag cttgatgata tcggccagcg
     7201 gctgaatgaa gcgcgggcca atgcggtttt gatactgggc caccagcttg cggtcaaccc
     7261 attcgtagag caggccggcc acaaacaaga acaaccctcc cgggaaaaac aggagcagaa
     7321 tgatcggatg caggctaagt tccataggtt ctcctcaatc ctagcggtcg ttgcacgaga
     7381 agcacgggtc aatgccggcc aggatcatcg gcatatcggc cagtttgtag cccggggcca
     7441 ggttgatgac cgaggcccag ttgcacagcg atggggtgcg gatcttgacg cgctcgggca
     7501 tgtcggtgcc gttgctcttc atataataga agagctcgcc acgcggggct tccacccgtt
     7561 ggatgatctc attttcgggg atgcggcggg gtacgcgcac cgccagctcg ccctcgggca
     7621 ggtgatccag aatttcgcgg accaggcgga tgctttcctg catttctttg atgcggacca
     7681 caaagcgggc cagcaggtcg cagccttcat ccacaatcag attgacgggg aagtccacat
     7741 agccagagta gggcgagtcc acacgcacat caatcggcac gccggaggcg cgggcggtgg
     7801 ggccaacggg ggccagggtt tcggcctgtt cgcgggtcat cacgcccacg tcgcgggtac
     7861 ggcccaggaa ggtctcatcg gtggtaacca cggtgaggta gtgtgccatg cggccttcga
     7921 ggaaatccat gcctttgagg atggcggttt tgatttcgtc gttcacatcc tgcttgacgc
     7981 cgccgatgat gttgatggaa tagttgacac ggttgccggt catctgttcc agcagatcca
     8041 tcacggtttc gcggtcacgc caggaataca tgaagagggt atcgaagccg ccctcgtgag
     8101 ccgcaacacc cagccagagc atgtggctgt gcaggcgttc cagttcagag gtcagcaccc
     8161 ggatggcctg ggcgcgcggc ggagccacca ctttggccag tttttcggtg cccagggcaa
     8221 acgaaagggc atgcacatgc gagcagatgc cgcagacgcg ctccaccagg tagagcccct
     8281 ggggatagtt gcgcgcttcg gtggcttttt cgatgccgcg gtgcacataa cccaggcgca
     8341 cactggcgcc ggtgacaatt tcaccatcaa cagtgaattc aaagtgagcg ggttccttca
     8401 gcgcagggtg ctgagggcca attggcacca caaatttttg cggagattcg ggattttcca
     8461 tgggagctct cctttattcc tgattcaaac cagtaaaaga tttccgcaag ggatagacat
     8521 ccgcgggcca ttcatccggc aggatgaggt gaccggggtc gggagtgcca aggcagtcta
     8581 cgccaagcat ttcgatcagc tcacgctcaa agagcgtggc tgcggggatg atatcgcaga
     8641 tggtggggac ttttgcgtcg gtatagggca cacgcacccg cagggttgcc agtgcgccgc
     8701 cattggcaaa ggtgtagagc acttctacat cgccggtggt ggctgcgcct tcggcggcag
     8761 gggcgggctt atccacgccg gtgatggctg ccaggtagcc ccagtgggca tctaccaggg
     8821 cctgcaccgc gggcttcagg ttgtcgaggg cgatgtagac atccacccgt tcgatttcgg
     8881 ggcgtttgat ttccacagcc aggggctgca gcagcgcttc agcagattgc agagccgttt
     8941 caaggtccat gtatgtctcc tcctactcag cgggctggac gggggctgga gccttttttt
     9001 gcaggctttg cagcagcgcc accacgccat agatgatggc ttcggggcgg ggcgggcagc
     9061 ccggcacata ggcgtccacg gggatcacct cgccgatgct gccgacgaca ttgtagcagc
     9121 cctggaaggc gccgcccgag atgccgcacg agccaaccgc caccacaaac ttgggttcgg
     9181 gcatttgctc gtagatgcgg cgcaggcggt cacgcgattg caggctgacc ggcccggtgc
     9241 agatgaggat atcggcgtgg cgcgggctgc cctgcagttt gatgccaaac cgttccagat
     9301 cgtagcgggg ggtgagggtt gccagaattt caatatcaca accattgcag gagccgctgt
     9361 tatagtggat ggcccagggc gagttgaccc gcgcccaggt cttgatctgt tccagcgccc
     9421 gctgccaggg ggtatccagt ttcagttcag tcatagggtt ctccttacca gtgattcaga
     9481 ctaactatac ctgatttgtc cgctggtttc aacccagcag cagcgccacc agggcgaggc
     9541 ccagccccac cagatagggc accatggacc aatccagcgt accggtggcc agcaccagca
     9601 cacccaggtg gagaatggcg aagaaaaacg cgatcaggaa gaaggggcgg tagcccggcg
     9661 cagcgatctg cgtgggggcc agttcaccac tgccgtacag cgagcgcttg agcggcgaac
     9721 cgggtttttc ggggccagcc aggcccttgc caatccagaa aatgatcagc accaggggga
     9781 tgtagatgag aaaggcgagc gggggggtga gaataatatc catgctcgtt ctcctttagg
     9841 ggacaatgat ccggagcaga gcctgcccgg ccagattacc gatgccgttg aagagggccg
     9901 gccagaagcc gaacaccagc accaccagcg agaggatgac tacgggaacc gtcatcagcg
     9961 cgggcacgcg cttgccagca gccactgcct cagaaggctc attgcgcacc atgcggttga
    10021 ccagcggggc atagtagccg agggagagca cactgttgag ggcggcaaag cccaccagcg
    10081 caagcatcca ccagttattg ctgccagccg cgccgacaaa gatctgccat ttggacatga
    10141 agcccgccag cgggggcagg ccgcccaggc ccagcaccgc aatgctgagg cccatggcaa
    10201 tcagcggata gcgtttggcg gcgccgttca ggtcggcaac ggtcagcggg tcgtgatcgc
    10261 ccttagcgat gtgcagcgag tagagcagcg cgcccgcgga gaggaaggcc aggcctttca
    10321 tcatcgcatg ggtgaagagg tggaagaacg cgcccgaacc gccctggtca gagaggaagg
    10381 tggcggtagc gccaatgccc accagcatat agccgacatg cgccaggctg gagtaagcca
    10441 gcaggcgttt gaccaccgtc tggcgcagcg ccatcaggtt gccaaccacc atgttcagca
    10501 cgccaaagcc cagcatgatg gggccccagg ccatgccgtt gtaggccatc acgcccatcg
    10561 agcgcagcag ggccaccaga ccggcggcaa tcaccacgcc cgaaagcatg gcgctgatgc
    10621 cgctgggggc ctgcgagtgc gcatcgggca gccaggtgtg catgggaacc agggccgtct
    10681 tgacgccaaa gccgatgatc atcagggcaa ctgccacagt aatgatcggc aggccagcag
    10741 cgccggcgcc catcagggcg agctgctgca ggttcagcgt acccgcgccc ataaagatca
    10801 ggccgatgcc cagcagcacc agggccgagc ccacggcgct ctgcaccagg tatttgaagc
    10861 ccgcttcaag cgaggcgggt tcatcccggt agaaggccac cagcatgaag gaggtgatag
    10921 ccatggtctc gaaccagacc cacaggttga agagatcagc ggcggtgccc aggccaatca
    10981 tggcggccat catggccgag agcagggcgt agtatttttc ttcgcccact tcgcgggcca
    11041 tgtaggggcc ggagtagagc atgaccatca gacccagcag ccagacggag attgccagca
    11101 caaggctgat gccgtcaaag cgcaggggaa tctctgcaaa gagcagacca acaacccctt
    11161 gaccagaggt cagcaggttt tgcgccagca caaagagcgg cacgcccatg gcggcggtgg
    11221 tgatgagggc aacccagcgt gccgggttgc gggggctcga ttcgccatcg cggcgggcca
    11281 cactgcggcc gatcagataa atgactggag aagccaggag gggcaggcca atcagccaga
    11341 ggatagcaaa gacagggttc atgggttacg ctcccagggc taaaatggca atcacgatca
    11401 gcaaaccggc cacaatgccc acaacgttcc agttgagcag gccggtctga gtgatgcgca
    11461 ggccttcggc ggcgccgcta acgccccgca caatgccgcg gttgatcgcc tcaaagccga
    11521 agctattttc agccgcccag cccagaccgc gcagcgcccg gccaagtccg gccagtttgc
    11581 tgcggaagac ccaggccagc aggcccagtg ccaccacagc cagcgcaatc agggtcggca
    11641 ggctgatgac ttcttccaga atgtggaagg tggaagccgc ctcgatgccg tgatagggca
    11701 gggtttcggc gctcagcagt ttggcgaagg ggccagccag cagccaggtg gagagagcgc
    11761 ccagggccag cggggccagg gcgatcttca tggctgtacc ggcatcacca tgggcatggc
    11821 cgtgagcttc accgtagaag accatggtga ccatgcggta ggtgtagaag gcggtcaggc
    11881 cagcgccaat gagcatgagc acatagctcc agatgggggc gtgcatcagg ccagcttcaa
    11941 gcaccagctc tttgctccag aagccattga ggatggggat gcccgccagc gccagtgcgc
    12001 cgatgatgaa gaccacgcgg gtgaagggca tttgcttgcc cagcccgccc atctggcgca
    12061 tatcccgcgt gcccacggcg tgaatgaccg cgccggcggc aaggaagagc agtgccttga
    12121 agacggagtg gctcaacagg tggaactggc tggcaaagat gccgcccgaa cccactgcat
    12181 agaccatata gcccaactgg ctgacggtgg agtaagccag tacacgtttg agatcgttgg
    12241 caaccacggc catcagggca gccaacaggg ccgtaatcag gcccaccagc atcaccgaga
    12301 aggtccagcc cggcacctca tggaaggccg ggtagaagcg ggccagcaga taaacgcccg
    12361 cgttgaccat cgtggccgca tggatgaggg cgctgatggg ggtgggagct tccatggcat
    12421 cgggcagcca ggtctggaag gggaactggg ccgatttggc cgcagccgcc accaggaaac
    12481 cgaaggccat cacgggcaag agctgcgccg gaatggccgc cgggtttgcc agcaggatgt
    12541 tatagtccat ctgcccggtg tagatgtaga gcaccagggc acccagcagc aggccaatgc
    12601 cgccaatctg ggtcatgatg agggctttga cgccgcccgc caccgccttg ggatcatcac
    12661 tgttgaagga gatgagggcg taggaacaca gggcggtaat ttcccagaag acgaacatga
    12721 gcagcatgtt gctggtgagc accaggccaa ccatggcgcc gatgaagaag agcatgtagg
    12781 cgtagtagcg gctgagctgc ttttcgccgt gcatgtaatt gaccgagaag atgaccgcca
    12841 ggctgccgat gaaggtggca accacggaca ggaagacgcc caggccatcg ggaacgaagc
    12901 tgatggggcc aaaaacgccg cccaggtcgg ctaattttac cggctcggag ccggtttgca
    12961 gcagcaggcc aaggcccgca accgccgaca ggacggcaaa aatgaccgcc agggtgtggc
    13021 tgagcttttc ggtgcggtca ccggcagccc agaccaccag tgcgcccagc cagggcaggc
    13081 ccagtgtaag caggatgaga atcgcaggtg tactcatggt tctacctctt caaccgggtc
    13141 aggtctttga catccagcga gccaaagcgg cggcggatct gcactgccag cgtcaggccg
    13201 atgaccgcca caatggtatc ggcaacaatg acagtcagcg ccagggtttg acccaggccg
    13261 ggctgggctg tcattttgcc agccagcacc agcaccagca tggcgccttt gaccatgatc
    13321 tgcagcgcca caatcacttt gatgagattg cgggtggcca gcagggcata aatgcccacg
    13381 ccgagtaaga gaatggccac gcccagaata atatgaggaa tatagttgct catgaatgct
    13441 cctcatctga cccgggggcc tcagccgccg ggcgcttctt gccttcagcc agcaggccca
    13501 tcatacccag tacgccggtg aagatgatgg cgacctggag caaaacatcc agatagcgcg
    13561 aggtccagag ggtggcataa aaggtctcgc cataatccgg gggcatttcg ccgcccatca
    13621 gtttggagag aaccatccag cctaccaggg ccagcaggcc aattgccaga atccagggga
    13681 tgggtttggg cagagccacg ggcagggcag tggactcatc gccggcgata ttgatcgcaa
    13741 agacgaagag cacggtgacc agtccggcgc ccacacttaa ttcgatgaca gcgatttccg
    13801 gcgcgccaag caggtacata agaagcgcaa ccagcgcact gcagccagcc agccagatgg
    13861 ctgaaaccag caagcgtttg gcgctgatgg ccagggcggc gcataccaga gctcccgctg
    13921 cagccaagac gtaccaaatg atctccattg gtcctccttg cagggtgagg ggataaaacc
    13981 ttatcttatc gcttataccg cagaataaga catttgtcat gtgaaaggta tcttaatttg
    14041 ataacctttt tatcctatta atcacaccgg agcgctccga aacggctctt accgggccta
    14101 cacaccgggt tggaggtagg gtttgaccac ctcc
//
LOCUS       DAOPVS010000044        14041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4442_length_14041_cov_55.662019, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000044 DAOPVS010000000
VERSION     DAOPVS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4442_length_14041_cov_55.662019"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..176)
                     /locus_tag="PKL11_05470"
     tRNA            complement(105..176)
                     /locus_tag="PKL11_05470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Lys,seq:ttt)
     gene            360..1070
                     /locus_tag="PKL11_05475"
     CDS             360..1070
                     /locus_tag="PKL11_05475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNZ13087.1"
                     /translation="MTTMQTEDEEILRLMEQMHAESKPIQMLNIFRGLPISYHALIDH
                     IEMGYVHLKVHGYQAVAIALEKRTFVQGGLLPEPVRANPVAVSVASQEVTLNRFAYAK
                     SPVGQRTTPRVQPKEPLKVEVRTSKGQISAMIADLSASGMGVFTFGTYIDYRLDLKRN
                     DQVELCIHLPGGRTLCLPGAISNMKRERSTMLYRLGLETYPDPIAAAALSDYVANRSA
                     EILQELETMYRTMCDAIA"
     gene            1100..2173
                     /locus_tag="PKL11_05480"
     CDS             1100..2173
                     /locus_tag="PKL11_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13088.1"
                     /translation="MPNQSPTPVEQMARDISQAQSNLGSLQTKARLSSLLDQVEDLQS
                     DIAGLPTVLADLRQRGYVYEKKLETTAQTMADRWRTLAPGIRTQIQIQSQAMMQAMPA
                     IETKMSSLARVSTSLGQAKPLLAQVEAEIRTMEGRANAAESTIRGMYNQFQSEVSTFK
                     AHLDRVAEAFKELSQASFQLLATEALIMSVEATWVRDAKEDDNDPKGNLYITDQRLIF
                     EQKQEVATKKVLFITTEKKLVQQLMFEVALALIEDVKPSKRGAFKNEDHLDLRFASGA
                     PFQTVHFHLFGQDCEEWVALIQRAKAKEFDADRVVAVDAAVEEKVKTAPTTCPNCSGA
                     ITKPVLRGQDTITCEYCGSVIRL"
     gene            complement(2160..2873)
                     /gene="lipB"
                     /locus_tag="PKL11_05485"
     CDS             complement(2160..2873)
                     /gene="lipB"
                     /locus_tag="PKL11_05485"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNZ13089.1"
                     /translation="MRKGHKVCTVHWLGQIDYLRAWQLQDQLAAEIADGKRPPTLLML
                     EHPHTYTIGRRGQAGNLLWDKDQLDALGIQVHEVDRGGDITYHGPGQLVGYPLLPLAA
                     PDWGQQRIPQADFVGYVRKLELGLIAALAELGVPAMTRDGLTGVWVAGPPSGKIVSIG
                     VKVDHRGISRHGFALNVAPDPHYWEGIVPCGLSGVKMVSLADYLNPVPGVAETAQVVA
                     RAVGSVFDYDMQFETFTIE"
     gene            complement(3001..3891)
                     /locus_tag="PKL11_05490"
     CDS             complement(3001..3891)
                     /locus_tag="PKL11_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ13090.1"
                     /translation="MTSLTPIHLSNGHKPVLLAVMAHPDDETFGTGGTLAVYARRGVA
                     VYLICATRGEVGEVDEKYLRGFDSIAERRMAELRCAAEKLGITEVMFLDYRDSGMPGS
                     PDNQHPQALAAQPVEKVAQQVAQIIRKLHPQVVVTFDPYGGYGHPDHIAIHKATVQAV
                     ALASDAGFVDPTHLTTHPIQRLYFHTPARGMLRIMVKLMPLFGKDPRKFGKNQDIDLA
                     AIAKQHFPVNARINYRVVADVREAAAACHASQGGGQQQRKGIMSVVLGIFSAKEEYMR
                     AIPAPRPRERVESDFFRDVV"
     gene            complement(3899..5056)
                     /gene="moeB"
                     /locus_tag="PKL11_05495"
     CDS             complement(3899..5056)
                     /gene="moeB"
                     /locus_tag="PKL11_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNZ13091.1"
                     /translation="MITQSLSQDEVFRYARHLVIPEVGMEGQKKLKEASVLIVGTGGL
                     GSPISLYLAAAGIGRIGLVDYDVVDRSNLQRQIVHDSDAVGQAKVESARRRLVGINPL
                     IQIDAYNTVFAAENAPEIARGYDVLVDGTDNFATRYLLNDLAALTKRPYVYGSIFRFE
                     GQVSVFDAQTGPCYRCLFPEPPPPNSVMSCTAGGVFGVLPGVIGSLQATETIKIILGI
                     GEPLIGRLMLYDALEMNFQIVKLRKNPRCPLCGETPTITRLVDYADFCGTPLPGGENE
                     TLPEAWEITPEALGKLLKSANPPLLVDVRQPMEQQIAQIPGAICLPVEELAARYPELP
                     HDRTLVLFCRTGIRGARAVRFLRDNGYTKAMRLVGGINAYAQAVDPTLLQY"
     gene            5120..7777
                     /locus_tag="PKL11_05500"
     CDS             5120..7777
                     /locus_tag="PKL11_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNZ13092.1"
                     /translation="MQIVLTHEQADFDAIGALLGAALLQDHTIPVLPRKMNRNVRAFL
                     NLYGSEFPFVEPRDLPQEHIDSVTLVDTQSLVTLKGISAATRVQVIDHHVRRPGLEPG
                     WQVMTERVGACTTLLVESLREHNGGLGGLHATLLLLGIYEDTGSLTYVSTTSRDVRAA
                     AYLLEQGASLQVVSEYLNPPLSPEQRLVYDRLLSSAHSHTINGQTIVIAATAAEEMTE
                     EISSVAHKLRDLLDPDALFILVGTIEGIRMVARSTTDQINVSSIAAEFNGGGHERAAS
                     ALIRPETLPSSDLPPLEYVCARLLEILPRYVRPSLTVGQIMSSGKIGTLSPETSAQEA
                     EKLMRRYGYEGYPIVQDGRVLGLLTRRAVDRALSHRLNLTAASLMEAGEITVSPGDTL
                     DHLQRLMASTGWGQIPVVGPDGRMIGIVTRTDLLKFLGSRLPSQPGRKNLAERLSSTL
                     PPAQLALLEQIAKAANTLHFPVYIVGGFVRDLLLDLPGLDLDIVVEGDAIALANQLNH
                     DFGGRVVAHHRFGTAKWQIKEIHAHLLRKFDLPPDVEGLPDALDLITARTEFYDHPTA
                     LPTVERSSIKLDLHRRDFTINTLALRLDGRHYGDLYDYWGGLGDLDNHLVRVLHSLSF
                     IDDPTRMLRAVRFEQRFDFEIEARTRELMSQAHDLLRQVTGERLRHEFDLIFIEPRAA
                     AMMERLENLDLLQAIHPALSWDTERTTIFDLLTRPPEPIWLLPDKIGGLPLRCGLGYL
                     AWLGRYPVEMLDAIGDTLRFSHPLLSVLKHHSRLIRELPTLAQARPSEITVRLDDVPL
                     IAIYALAAAADPVSEYPALAAYITHWQKIQPLTNGLVLRQLGLPPGPTYRSVLSRLRS
                     AWLDGEIHTPQEEQKLLDQLLAEYPGALA"
     gene            7774..8313
                     /locus_tag="PKL11_05505"
     CDS             7774..8313
                     /locus_tag="PKL11_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13093.1"
                     /translation="MSTSPLPQPTQPVPWLQSVLIRPALRYDLPALEWEGEFSHFRRV
                     FEQAYDRMLNKKSIIWVADLQGQIIGQVFIQLDCDRPELADGHDRAYLYSFRIRPSYR
                     GQGLGSQMIAVLEADLRVRCYQILTLNVAKDNLKAQQLYQRLGFQITAHEPGRWSYPD
                     QHGVWHQVEEPAWRMEKHL"
     gene            complement(8310..8864)
                     /locus_tag="PKL11_05510"
     CDS             complement(8310..8864)
                     /locus_tag="PKL11_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13094.1"
                     /translation="MFTHKQSKFLVLSLLTVFALLITGCTATIEPTAGPTPTPTEGQC
                     AFVWATQNLEESSKKLQTALDEAGLTIVTGNAGVFGENCVNEDGTIQGFAARDVDIYL
                     SFTVDNLEDTAALGSLAIKATEVLMALPAETFPKTIDFVNYHFTDGTNSIYAQQTYTG
                     LVALMESKPTGQDFYNAIQKPAPF"
     gene            9010..11082
                     /locus_tag="PKL11_05515"
     CDS             9010..11082
                     /locus_tag="PKL11_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ13095.1"
                     /translation="MKEYTTDSIRNVALASHSGLGKTMLAEAFLHFSGATTRLGKIED
                     GTTISDFDEEEVRRGISIYTSVLPVEHKNVKINFLDTPGYNDFIGEVISALRVADSAI
                     LLVDAVSGVEVGTEIAWDYCNRFNLPRFVVINKMDRDNASYAKALAAIEEFSDLRLVP
                     IQLPWGEKAAFQGVIDLLTMKAYKGDGKTVVDIPADLRAAADEARVKLMEAAAEGDDT
                     LMEKYFEAGELSGEEILRGLRAGVINRSFAPVFVSAASAEIGIGPLLDAIVTLFPSPA
                     EAAKAVAKGKDGDETLTAADSGPLAAYVWKTTADPFVGKQTFFRVYSGMVSSDSRVWN
                     QNKSIEERFGTISVPRGKEIISIKVLHSGDIGVVPKLSETSTSDTLCDKAHPLTLPRP
                     DYPGALYRVAVNPKTQADSTKLSSTLTRQCEEDMTLSWHNDISTNQTILQGMGDQHIE
                     VAIRRAETRFQVGIITTEPRVPYRETITRKGSAMYRHKKQTGGSGQFGEVHLRVEPFP
                     EGDFDFVWEVFGGAVSQSYANSITKGIQSVLKEGVIAGYPVSGVRVAVTDGKEHPVDS
                     KPIAFEIAGREAFKLAFKEAGAMLLEPIMMVKIMVPEANMGDVLGDLNTRRARVMGMD
                     TDRGHSTITATVPLAEMQRYTTQLRSITGGRGIFSMEFERYENVPPHLTQELVAIRQK
                     ELESKKEE"
     gene            11133..11339
                     /locus_tag="PKL11_05520"
     CDS             11133..11339
                     /locus_tag="PKL11_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13096.1"
                     /translation="MLPDPLEVLWDNLLSRQPERIRLAFGALDEAGRRAALEHLRRMA
                     TEPDWHPEQRASALAALKALDQRR"
     gene            11348..12541
                     /locus_tag="PKL11_05525"
     CDS             11348..12541
                     /locus_tag="PKL11_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNZ13097.1"
                     /translation="MKTSRVISAKNTLLFLLLLALVLSAGLTWAIHRVMQGNTPGMDF
                     YIFWLAGRAALTGGNPYGDDIGVAVQLGTYDHLSGAGQNQMLFAYPPYALLAVLPFLA
                     LPFDWAQAAWLSVNLIALAATYLALKPVSPLRSIAGGLLFFPVAFALILGNFVVLIGA
                     ILLLVLTSMIQARQHSPAGWVALGILLAWTTIKPQFSWLFLAFIAVWVLRSRTWHLAW
                     GFLGGLVTMLALSFVVVPDWLSLWLGVLQKYTTYHPAEPALATFLNAWLPHGAAQPLT
                     IVIIAVIAALTLILFRRWWQGRETPLRLLAWLGLAAYLLAPQSVSYEQVRFLLPLFLW
                     AAMLPAQNRWQLWIFFWGGLLVSWGVFVIERTNISFALNSGWRLLYHLGWLIWLFWPF
                     NRRKS"
     gene            12541..13743
                     /locus_tag="PKL11_05530"
     CDS             12541..13743
                     /locus_tag="PKL11_05530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNZ13098.1"
                     /translation="MQKPGIRLLIILLAVSIITGLLSVGIHAALGNNRIGADFYFFWT
                     ASRAAFIEGQSPYSPEVTAQIQQGIYGRSAEPSEDQMIFAYPAHSLLLVGPLAWLPFD
                     WAQALWMALGIIGLVLLPLVLFPRAPLWVRLSLILYYPVAFALILGNFNLPVMLALLV
                     FWSRFTIRESHPASAEQISLGFLLSIATIKPQLVWAFVLLAVLLAFRRRTYILLGSFA
                     GFTLLFLLIPFTFIPNWAGEWLAQIQYYTTFEHAGGDTVQVMLLQFLSLIGLNGWAAT
                     AIAGLTGLAGLIGLLCLVFRKSSRPAAPLIALGLAGLLIYVLHPSGLSYEQLAMILPM
                     FIWAALRGAPRPAVLFFWLGGLLLSWGVFSLARSLSQNWILYLPPTLWTLAWWLWLLL
                     ARRRPAQT"
     gene            13753..>14041
                     /locus_tag="PKL11_05535"
     CDS             13753..>14041
                     /locus_tag="PKL11_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNZ13099.1"
                     /translation="MDISELTAHMQAFVTAKGWYAPTSLRPQTPRNIAISLNLESAEI
                     LEHFQWGEESRDPDGLPGELADVTLYLLQLAHVLNIDLEQAVLQKLAVNYGR"
BASE COUNT         2895 a         4350 c         3548 g         3248 t
ORIGIN      
        1 gggacgagca accaatctct tataagtaaa cctagtgagc gaggagaggc tcgaatacaa
       61 tccctcgatg ggacgagcaa ccaatctctt ataagtaaac ctagtgagcg aggagaggct
      121 cgaactctca accaatggct taaaaggcca ctgctctgcc attgagctac tcgcccgaca
      181 gccagaattt tatcacgcac tgcctttaac gtcaaaggat tgcccctcgc aaacaacaaa
      241 ttaaatcctt gattaatttg ccccaaaaat ctttgcccat ttttatgaaa tcactatata
      301 atcaaattgc aaggccaact catagtttgg tcaggtattt acctgtgctg gagcaaccaa
      361 tgaccacaat gcagacagaa gatgaagaaa tcctgcgcct gatggagcag atgcatgctg
      421 aaagcaagcc catccagatg cttaacatct ttcgcggctt gcccatttcc tatcacgcgc
      481 tgatagatca tattgaaatg ggctatgttc acctgaaagt tcatggctat caagctgtag
      541 ccattgcgct tgaaaaacgc actttcgttc aaggtggcct gttgccagag ccagtccgcg
      601 ccaacccggt tgccgtcagc gttgccagtc aggaagtcac cctcaatcgt tttgcgtacg
      661 ccaaatcacc ggtggggcag cgcaccaccc cgcgcgtcca gcccaaagaa ccgctgaaag
      721 tcgaagtgcg aacctcaaaa gggcagatct ctgccatgat tgctgacctt tctgccagcg
      781 gcatgggcgt ttttaccttt ggcacctata tcgactaccg cctggatctc aaacgcaacg
      841 atcaagttga attgtgcatt caccttcccg gcggccgcac cctttgcctg ccgggcgcca
      901 tctcgaacat gaagcgtgaa cgctccacga tgctgtatcg tctgggactg gaaacctatc
      961 ccgatccaat tgccgctgcc gcattgtcag attatgtggc taatcgttct gcagaaatct
     1021 tgcaggaact ggaaaccatg tatcgcacca tgtgcgatgc cattgcctga aaccccataa
     1081 aacaattgag gaggaattca tgcccaatca atccccaacc ccggttgaac aaatggcgcg
     1141 cgacatatcc caggcccaaa gcaatctggg ttcgctgcaa accaaagccc gcctgagcag
     1201 tcttctcgat caggttgagg acctgcagtc cgatatcgct ggtttgccca ccgtcctggc
     1261 tgatttacgt cagcgcggct acgtttacga gaaaaagctg gaaacaaccg cccaaaccat
     1321 ggctgaccgc tggcgcacat tggcgccggg tatccgaaca caaatccaaa ttcagtccca
     1381 ggcaatgatg caggccatgc ccgccatcga aaccaaaatg tcttccctcg ctcgggtttc
     1441 aaccagtctg gggcaggcca aacccctgct cgcccaggtt gaagctgaaa ttcggaccat
     1501 ggaaggccgc gccaatgcgg ctgagagcac catccgcggc atgtataacc agttccagag
     1561 tgaggtgagc accttcaaag cccatcttga tcgcgtcgcc gaggcattca aagaattatc
     1621 gcaggcttcc ttccaactgc tcgcaaccga agcgttgatc atgtcagtcg aggcaacctg
     1681 ggttcgcgat gccaaagaag atgataacga ccccaagggc aacctctaca ttaccgacca
     1741 gcgcctgatc tttgaacaaa aacaggaagt tgccactaaa aaagttctct tcatcaccac
     1801 cgagaaaaaa ctggtccagc agctcatgtt tgaagtggct ttggccttga ttgaggacgt
     1861 caaaccatcc aaacgcgggg cctttaagaa cgaagatcac ctcgatttac ggtttgcctc
     1921 tggcgcgccc ttccaaacgg ttcacttcca cctgttcggt caggactgcg aagaatgggt
     1981 tgcgctcatc cagcgcgcta aagccaaaga atttgacgcc gatcgagtgg tcgcggttga
     2041 tgccgcggtg gaagaaaagg tcaaaacggc gcccacaacc tgccccaatt gctccggcgc
     2101 aatcaccaaa cccgttctgc gcgggcaaga taccatcacc tgcgaatatt gcggctcggt
     2161 tattcgattg taaacgtctc aaactgcata tcgtagtcaa acacactgcc aaccgcgcgg
     2221 gcaaccacct gcgcggtttc tgctacacca ggcaccgggt tgagataatc cgccagtgaa
     2281 accatcttga cgccggataa tccgcagggc acaatccctt cccagtagtg cggatccggc
     2341 gctacgttca gcgcaaaccc gtgtcgtgat atcccccggt gatccacctt caccccaatg
     2401 gacacaattt tcccggatgg tgggcccgct acccatacac cggtcaatcc atcgcgcgtc
     2461 atagctggca cgccaagctc tgccagggca gcaatcaacc ctagctccag tttccgcaca
     2521 tagcccacaa aatcggcctg cggtatccgc tgctgccccc aatccggtgc agccagcggc
     2581 agcagcggat acccaaccag ttgcccgggt ccatggtagg taatatcccc gccgcgatca
     2641 acctcgtgga cttgaatccc cagcgcatct aactgatctt tatcccacag cagattaccg
     2701 gcttgcccgc gtcgcccaat cgtgtaggta tgcgggtgtt ccagcatcag cagggtgggc
     2761 gggcgcttcc cgtccgctat ttctgcagcc agctgatcct gcaactgcca ggcgcgaaga
     2821 tagtcaatct gccccagcca gtggactgta caaaccttat ggccttttct catttacatt
     2881 tcatgcaaaa atgatagaat tgggttagaa tactctcgtt actaatcaaa ggacttttct
     2941 cctcttctat taccttctct ccgcccaggc tcggagagaa gggcttttaa cccccaaacc
     3001 ttaaaccaca tctctaaaga agtcgctttc aacccgctcg cgtgggcgcg gtgccggaat
     3061 ggcgcgcata tactcctctt tggcgctgaa tatccccagc acaacactca taatgccttt
     3121 acgctgctgc tgcccgcccc cctggctggc gtgacacgcc gccgccgcct cgcgaacatc
     3181 cgcaaccacc cggtaattga tccgtgcatt caccggaaaa tgctgtttgg caatggcagc
     3241 caggtcaata tcttgatttt tcccaaactt gcgcgggtct tttccaaaca agggcattaa
     3301 tttcaccatg atccgcagca tcccccgtgc cggcgtgtga aaatataacc gttgaattgg
     3361 gtgtgttgtc aggtgggttg ggtcaacaaa gcccgcatca ctcgccagcg ccacagcctg
     3421 cacggtggcc ttgtgaatgg caatatggtc aggatgccca taccctccat aggggtcaaa
     3481 ggtcaccacc acctgggggt gaagttttcg gataatctgg gccacttgct gcgccacctt
     3541 ctccacgggc tgggctgcca gcgcctgcgg atgctgatta tcgggtgacc ccggcatgcc
     3601 cgaatcgcga tagtcgagga acatgacctc ggtaatcccc agcttttcag ccgcgcagcg
     3661 cagttctgcc atccgccgct cggcgattga atcaaatccc cgcaggtatt tttcatccac
     3721 ctcgccgacc tcgccgcgcg tcgcacaaat caaatagacc gccacgccgc gccgggcata
     3781 aaccgccagc gttccgccgg tgccaaaagt ctcatcgtct gggtgcgcca taaccgcgag
     3841 cagcacgggc ttgtgtccat tggataggtg aatgggtgtc agggatgtca tttagacctc
     3901 aatattgaag cagggtaggg tcaaccgctt gagcataggc attgatgccg ccgaccaatc
     3961 tcatggcttt cgtatagccg ttatcgcgca gaaatcggac tgcccgcgcg ccgcggattc
     4021 cggttcggca gaacaaaacc agcgtgcggt catgcggcag ttctggatat cgggctgcca
     4081 gctcttcgac tggcaggcaa atcgcccccg gaatttgtgc aatctgctgc tccattggct
     4141 ggcgcacatc caccagcagg ggtggattgg ccgatttcag cagcttgccc agcgcttcgg
     4201 gtgtaatttc ccatgcttcc ggcagcgttt cattttcgcc tccgggcagg ggtgtgccgc
     4261 aaaaatccgc ataatccacc aggcgtgtga tcgttggggt ttctccgcac agcgggcacc
     4321 tggggttctt tcgcagcttc acaatttgaa aattcatttc cagcgcgtcg taaagcatca
     4381 gccgcccgat taagggctct ccgatgccca ggataatttt gatggtctct gtcgcttgca
     4441 gcgatccaat cacccccggc agaacgccaa acacgcctcc cgctgtacag ctcatcacgc
     4501 tgttgggcgg ggggggctct gggaaaaggc agcggtagca gggtccggtt tgtgcgtcaa
     4561 acacgctgac ctgtccctca aaccgaaaga ttgagccgta cacatatggg cgcttggtca
     4621 gtgccgccag gtcattcagc agatagcggg ttgcaaaatt atcggttcca tcaacgagca
     4681 catcatatcc ccgggcaatt tccggggcat tttcagccgc aaagaccgtg ttataggcat
     4741 cgatctggat taacggattg atgccaacca gcctccgccg cgccgattca accttggctt
     4801 gccccacggc atcactgtca tgcacaattt gccgctgcaa atttgagcgg tccaccacat
     4861 cgtaatccac cagaccaatc cgcccaattc cggcagccgc caggtacagg ctgatgggcg
     4921 agcccagccc gcccgttcct acaattaaca cagaggcttc tttgagcttt ttttgccctt
     4981 ccatccccac ttctggaatc acgagatggc gagcgtaccg aaaaacctca tcctgcgaga
     5041 gcgattgtgt gatcatatat ttatgcattg atgataccaa aaacacgtta agctggcaaa
     5101 tcttgggtac aattgtttta tgcaaattgt gctcactcac gaacaggccg actttgacgc
     5161 catcggcgcc ttgctgggcg ccgccctctt gcaggatcat accattccgg ttttgccccg
     5221 caagatgaat cgcaacgttc gggcattctt gaacctttac ggctccgagt ttccctttgt
     5281 ggagccgcgg gacctgcccc aggagcatat tgattccgtc actctggttg atacgcagtc
     5341 tctggtcacc ctgaaaggca tcagtgctgc aacccgcgtc caggtcattg atcatcacgt
     5401 tcgccgcccg ggtctcgaac ccggctggca ggtgatgacc gagcgggtgg gggcctgcac
     5461 caccctgctg gttgaatcgc tgcgggagca taatggtggt ctgggcggtt tgcatgccac
     5521 cctcttgctt ctgggcatct atgaagacac cggctcactg acctatgtca gcaccacctc
     5581 gcgagatgta cgcgccgcag cctacctgct ggaacagggc gccagccttc aggtcgtctc
     5641 agaatatctc aatccgccgc tttcgcccga acagcgcctt gtctatgacc gcctcttgag
     5701 ttccgctcac tcgcatacca tcaacgggca aaccatcgtc atcgctgcca ccgcagcaga
     5761 ggaaatgacc gaggaaatct ccagcgttgc ccataaactg cgcgacctgt tggaccccga
     5821 tgccctgttc atccttgtcg gcaccattga aggcatccgg atggtggccc gctccacaac
     5881 cgatcaaatt aatgtttcca gcattgccgc agaatttaat ggcggcgggc acgagcgcgc
     5941 cgcctcggcg ctcatccgtc ccgaaaccct gccctcatca gacctgcccc cgttggaata
     6001 cgtctgcgcc cgcctcctgg aaatcctgcc ccgttatgtg cgcccttccc tcaccgttgg
     6061 ccagatcatg tcttccggca aaattggcac cctgtcgccc gaaacctctg ctcaggaagc
     6121 tgaaaagctg atgcgccggt acggttatga gggctatccc attgtccagg atggccgcgt
     6181 cctcggcctg ctcacccgcc gggcagttga ccgtgccctg tcccaccgcc tcaatctcac
     6241 cgctgccagc ctgatggaag cgggcgagat taccgtatcg cccggagata cgctggacca
     6301 ccttcaacga ctgatggctt caaccggctg ggggcagatc ccggttgtgg gacccgatgg
     6361 ccgcatgatt ggaatcgtca cccgcacaga cttgttgaag ttcctgggct cgcgtcttcc
     6421 ctcccagccc ggccgcaaaa accttgccga gcgcctctcc agtacactgc cgccagccca
     6481 actggcgctc ttggagcaga ttgcgaaggc tgccaatact ttacacttcc cggtgtatat
     6541 tgtcggtggc tttgtccgtg acctgctcct cgatcttccc ggcctcgatc tggatattgt
     6601 tgtcgaaggt gacgccatcg ccctggcaaa tcagctcaac cacgattttg gcggacgcgt
     6661 tgtggcgcat caccgctttg gcacggctaa atggcagata aaagaaattc atgcgcactt
     6721 gctgcgcaaa tttgacctgc cccccgatgt cgaaggcttg ccagacgccc tggatctgat
     6781 caccgcccgc accgagtttt atgatcatcc cactgctctc cccaccgtgg agcgcagcag
     6841 catcaagctc gaccttcatc gccgcgattt caccatcaac accctcgccc tccgcctgga
     6901 tggccgccat tacggcgacc tttatgacta ttggggcggc ctgggtgatc tggataatca
     6961 cctggttcgg gtgctgcatt ctctttcctt catagacgac cccacccgca tgttgcgtgc
     7021 ggtgcgcttt gagcagcgtt ttgattttga aatcgaggcg cgcacccgtg aacttatgtc
     7081 acaggcgcat gacctcttgc gccaggtcac cggcgaacgt ctgcgccatg aatttgacct
     7141 catctttatc gaacctcggg ctgctgccat gatggaacgc ctggaaaacc tcgatttgct
     7201 gcaagccatt catcccgccc tttcctggga caccgagcgt accacaatct tcgatttact
     7261 gacccgccca cctgaaccaa tctggctgct gcctgataaa atcggcgggc tgcccctgcg
     7321 ctgcggcctg ggttacctgg cctggctggg tcgctatcct gtggaaatgc tcgatgccat
     7381 tggcgacacg ctgcgttttt ctcatccatt gctttcagtt ttgaaacacc acagccgcct
     7441 catccgcgaa ctacccaccc tggcccaggc gcgtccctct gaaattaccg tccgtttgga
     7501 cgacgttccg ctgattgcca tttacgctct ggcggcggct gccgatcccg tgtcagaata
     7561 cccggctctg gctgcctaca tcacacattg gcaaaaaatc cagccgctga ccaacggtct
     7621 tgtgctgcga cagcttggcc ttccgcccgg tccaacctac cgttcggttt tgtcccgcct
     7681 gcgctccgcc tggctggatg gcgaaatcca cacccctcag gaagagcaga aacttttgga
     7741 tcagctcctg gctgaatatc ccggagcact tgcatgagca cctcaccgct gcctcaaccc
     7801 acccagcccg tgccctggct gcagtctgtg ctgattcgcc ccgcgctgcg ttatgacctg
     7861 cccgccctcg aatgggaagg cgaattttcg cacttccgcc gggtttttga gcaggcctat
     7921 gaccgcatgc tgaataaaaa atcaatcatc tgggtggctg accttcaggg acaaatcatc
     7981 ggccaggtct tcatccagtt agattgtgac cgccccgaac tggctgatgg acatgaccgt
     8041 gcctatctct attctttccg cattcgcccg tcttaccgtg ggcaggggct tggcagccaa
     8101 atgatcgccg tactggaagc cgacctgcga gtccgctgct accagattct gactttgaat
     8161 gttgccaaag ataatttgaa agcccagcag ctttatcagc ggctgggctt tcaaatcact
     8221 gctcacgaac ccggacgttg gtcctatccc gatcagcatg gcgtctggca tcaggtggaa
     8281 gaacccgcct ggcggatgga aaaacacctc taaaacggag ctggcttttg aatggcgtta
     8341 taaaaatcct gaccagtcgg ttttgattcc atcaacgcga ccagcccggt gtaggtttgt
     8401 tgcgcgtaga tgctgttagt gccatctgta aagtggtaat tgacaaaatc aatggttttt
     8461 ggaaaggtct cagccggcag cgccatcaaa acctcggtgg ctttgatcgc cagactgccc
     8521 agtgcggccg tatcttccag gttgtccaca gtgaacgata aatagatatc cacatcccgc
     8581 gctgcaaaac cttgaatggt gccgtcttcg ttaacacagt tttccccgaa gacgcccgca
     8641 tttcccgtaa caatggtcaa cccggcttca tccagtgcag tttgtagttt cttgctcgac
     8701 tcttccaggt tttgtgttgc ccacacaaag gcacattgtc cttctgttgg ggtgggggtg
     8761 gggcctgcgg tgggctcgat ggtggcggtg caccctgtaa tcagcagcgc aaacacggtt
     8821 agcaggctaa ggactaagaa tttggattgt ttatgtgtga acatgataaa aggtcacctt
     8881 ggttgaactt tgggatgtgt tataatatgc cagtttgcga aaaccgtcaa cgctgttctt
     8941 ggctatccac agcgattttt aagaattgat cgttgactgc agaactcatt caaagaagga
     9001 ttctggtgca tgaaagagta tacaactgat tcaatccgca atgttgcact ggcctcacac
     9061 agcgggctgg gcaagacaat gctcgcggag gcgtttctgc atttctcggg tgcaaccacc
     9121 cgcctgggca aaatcgaaga tggcaccacc atctccgatt ttgatgaaga agaggtgcgg
     9181 cgcggaattt caatttatac ttccgtcctc ccggtggaac acaaaaacgt caaaatcaat
     9241 ttcctcgaca cccccggtta caatgatttc atcggcgagg tcatctcggc tctgcgggtt
     9301 gccgacagtg ccatcctcct cgtggatgcc gtctccggcg tagaagtcgg tactgagatc
     9361 gcctgggatt actgcaatcg cttcaacctg ccgcgctttg tcgtcatcaa caagatggat
     9421 cgtgacaacg ccagctacgc caaggccctt gctgccattg aagaattcag cgatctgcgt
     9481 ctagttccca tccagctccc ctggggcgag aaagccgcct tccagggtgt gattgacctg
     9541 ctgaccatga aggcctacaa gggcgatggt aagaccgttg tagatatccc cgctgacctg
     9601 cgcgctgccg cagacgaagc ccgcgtcaaa ctgatggaag ccgctgccga aggcgacgac
     9661 accctgatgg aaaaatattt cgaggctggc gaactctccg gcgaagaaat cctgcgtggt
     9721 ctgcgcgctg gcgtaatcaa ccgatcattt gccccggtct ttgtctctgc cgcctctgcc
     9781 gaaatcggca ttggtccgct cctcgacgcc atcgtgactc tgtttccgtc gcccgctgaa
     9841 gctgccaagg cagttgccaa aggcaaagat ggcgatgaaa ccctcaccgc tgccgatagt
     9901 ggcccgctgg ctgcctatgt ctggaagacc actgccgacc cctttgtggg caagcagacc
     9961 tttttccggg tgtactctgg catggtcagc tccgacagcc gggtctggaa ccagaacaag
    10021 agcatcgaag aacgctttgg caccatcagc gtcccgcgcg gtaaagaaat catcagcatc
    10081 aaagtcctcc acagcggtga tatcggtgtg gttcccaagc tctccgaaac ctccaccagc
    10141 gacacgctgt gtgacaaagc ccacccgctc accctgccgc ggcccgatta ccccggtgcc
    10201 ctctaccgcg tagcggttaa ccctaaaacc caggccgact cgaccaaatt gagctctacc
    10261 ctgacccgcc agtgcgaaga ggatatgacc ctctcctggc acaatgacat ctctaccaac
    10321 cagaccatcc ttcagggtat gggcgaccag catatcgaag ttgccatccg ccgcgctgag
    10381 acccgcttcc aggtaggcat tatcacgacg gaaccccgtg tgccctaccg cgaaaccatc
    10441 acccgcaagg gcagcgccat gtaccgccat aagaaacaaa cgggcggttc gggtcaattt
    10501 ggtgaagtcc atttgcgcgt tgagcccttc cccgaaggcg actttgactt cgtctgggaa
    10561 gttttcggtg gcgcagtttc gcaatcctac gccaactcaa tcactaaagg catccagagc
    10621 gtcttgaaag aaggcgttat cgccggctat cccgtctctg gcgtccgcgt ggcggtcacc
    10681 gatggtaaag agcatcctgt tgactctaaa ccaatcgcct ttgaaattgc tggtcgtgag
    10741 gccttcaagc tggccttcaa agaagccggt gccatgctcc ttgagccgat catgatggtc
    10801 aaaatcatgg ttcccgaagc caacatgggc gacgtactgg gcgatctcaa cacccgccgt
    10861 gcccgcgtga tgggcatgga cactgaccgc ggtcactcca ccatcaccgc cacagtcccg
    10921 ctggctgaaa tgcagcgcta taccacccag ctccgctcca tcacgggcgg ccgcggcatc
    10981 ttctccatgg aatttgagcg ctatgaaaat gtcccgccgc acctgaccca ggaactggtt
    11041 gctatccgcc agaaggaact ggaaagcaag aaggaagaat aaaacctctc gctgccccct
    11101 tctcaagaag ggggcagctt tttaaggccc ctatgctccc cgatccactc gaagttttat
    11161 gggataatct gctctctcgc cagcccgagc gcatccgact ggcctttggc gccctcgatg
    11221 aggcgggccg ccgggcagcg ctcgaacatc tccgccggat ggctactgaa cccgactggc
    11281 accccgaaca gcgcgcctcg gcattagccg ccttgaaagc gctggatcag cgccgctaaa
    11341 cctgtttatg aaaacctccc gggtcatctc ggcaaagaat acgctgcttt ttctgcttct
    11401 gctggcctta gtgctttctg ccgggctcac ctgggccatc caccgcgtga tgcagggcaa
    11461 tacccccggc atggattttt acattttctg gctggccggg cgtgcggctc tgacgggcgg
    11521 caatccctat ggtgatgata ttggggtcgc cgtccagctc ggcacctacg accacctctc
    11581 tggggcaggc cagaatcaga tgctctttgc ctacccccct tatgccttgc tggccgtttt
    11641 gccattcctg gcactgccct ttgattgggc tcaggccgcc tggctctccg ttaatctgat
    11701 cgcccttgcc gccacctatc tggcgctcaa gcccgtctca ccgttgcgca gcattgccgg
    11761 gggcttgctc ttcttcccgg ttgcctttgc actcatcctg ggcaactttg tggttctcat
    11821 tggcgccatc cttctgcttg tcttgacctc aatgattcaa gcacgccagc attctcctgc
    11881 gggctgggta gcgctgggca tcttgctcgc ctggacgacc atcaaacccc agttctcgtg
    11941 gctgttcctg gcttttattg cggtctgggt gctgcgctcg cgcacctggc atttggcctg
    12001 gggctttctg ggcggtctgg tcaccatgct ggcgctgtct tttgtcgttg tcccagactg
    12061 gctttcgctc tggctggggg tgctgcaaaa atataccacc taccacccgg cagaaccagc
    12121 cctggccacc ttcctgaacg cctggctgcc tcatggcgcc gcccaacctc tgacgattgt
    12181 aatcatcgca gtcatcgccg cgctcacgct catcctgttc cgccgctggt ggcaggggcg
    12241 tgaaaccccg cttcgcttgc tggcctggct gggccttgct gcctacctgc ttgcccccca
    12301 atccgtttct tatgaacagg tccgcttcct gctgccgttg tttctctggg ctgccatgct
    12361 gccggcccaa aaccgctggc agctctggat tttcttctgg ggcggcttgc tggtttcatg
    12421 gggcgtcttt gtgattgaac ggaccaacat ttcatttgcc ctcaacagcg gctggcggtt
    12481 gctctatcac ctgggttggc tcatctggct cttttggccc ttcaaccgga ggaaatcata
    12541 atgcaaaaac ctggcattcg gctattgatt atcctcctgg cggtcagcat catcactggc
    12601 ttgttgtccg tcggtatcca tgccgctctt gggaataacc gcattggcgc agacttttat
    12661 ttcttctgga cagcttcccg ggcagccttt attgagggtc aatcgcccta cagtcctgag
    12721 gtcactgccc agattcaaca gggcatctac ggccgctctg ctgaacctag cgaagaccag
    12781 atgatctttg cctatcctgc ccacagctta ctgctggtcg gccctctggc ctggctccca
    12841 ttcgattggg ctcaggcgct ctggatggct ctgggcatta ttggcctggt gctgctgcct
    12901 cttgtgcttt ttccccgcgc cccgctctgg gtcagattat ccttaatcct ttactatcca
    12961 gtggcctttg ccctgattct gggtaatttc aacctgcccg tcatgctggc tctgctggtt
    13021 ttctggagcc gctttaccat ccgtgagtcg catcctgctt cagcagaaca gattagcctg
    13081 ggatttttgc tttccatcgc cactattaaa ccgcagttgg tctgggcctt tgtgctcctg
    13141 gcggtcttgc tggccttccg tcgccggact tatatcttgc tcggcagctt tgccggtttc
    13201 acgctcttat tcctcctcat cccgttcaca tttatcccca actgggcggg ggagtggctg
    13261 gcgcagattc aatactacac cacctttgaa cacgccggcg gcgatacagt tcaggtcatg
    13321 ctgcttcaat tcctatcctt aattgggttg aatggctggg ctgccactgc catcgctggc
    13381 ctgacagggc ttgccggtct gattggcttg ctctgcctcg tttttagaaa atcctcccgc
    13441 ccggccgcac cgctcattgc tcttgggctg gcgggcttgc tgatctatgt tctccacccc
    13501 agcggcctgt cttacgaaca attggcaatg atcttgccta tgttcatctg ggccgccttg
    13561 cgcggcgctc ctcgtcccgc ggttttattc ttctggctcg gcgggctgct cctctcctgg
    13621 ggtgtcttca gccttgcgcg cagcctgagc cagaactgga tactctatct cccccccacg
    13681 ctttggacgc tggcctggtg gctctggctt ttgctggccc gccgccgccc ggctcagacc
    13741 tgaaaggatt ctatggatat ctctgaatta acggcgcaca tgcaggcgtt tgtgaccgcc
    13801 aaaggctggt atgcccccac atcgctgcgc ccgcaaacac cgcgcaatat tgccatatcc
    13861 cttaatctcg aatccgccga aattttggaa cacttccagt ggggcgagga atcccgcgat
    13921 cccgacggcc tgcccggtga actggcagac gtcaccctct acctgctcca actcgcccac
    13981 gtccttaaca ttgacctgga acaggccgtg ctgcaaaaat tggcagtcaa ttacgggcgc
    14041 g
//
LOCUS       DAOPVS010000045        13665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4594_length_13665_cov_41.756429, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000045 DAOPVS010000000
VERSION     DAOPVS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13665
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4594_length_13665_cov_41.756429"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PKL11_05540"
     CDS             complement(<1..860)
                     /locus_tag="PKL11_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13100.1"
                     /translation="MSFNLKTTLKRLMAPLDLDAQRRKNYINVQVDGVGVGLANAASP
                     FLPVFLARLQASSYEIGLLSSMPAITGLLLSIPLGQFLQTRRNVVPWYSVARVLVIAG
                     YALSGLAAFFLRGEALVWGILAIWALVTIPQTVLAICFSVVMNAVAGPNGRFELMSRR
                     WIILGFTTAVTVFLIGQLLDNPRLLFPVNYQLAFLVLSLGGVLSFIFSRQIIIPSVER
                     SPGEKTNPLQNLSLVFSEKPFISFIVKRIIYSAGISMSLPLFPLYFVREIQATDSWIA
                     AIATVQSAII"
     gene            complement(850..3081)
                     /locus_tag="PKL11_05545"
     CDS             complement(850..3081)
                     /locus_tag="PKL11_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNZ13101.1"
                     /translation="MSDATHDLVQFLLTASQLPIEEAQRLALRGAVAFLEEAGTPEAI
                     GGLAEVARSGISVEAAAQALESLRRLSRSGTGEAIAKVFELAIQYNHPDAQAAVVVDN
                     LPAPEPGTQIVFWLLTGQAKESLRLDPTHRILTAKFTGAPPDLQARLLQAAQKAGWTG
                     WHALAAALVEDTPARYTELIQAFGALAPDEQGAVLDTVYETGQRGSAPAKEALCELYL
                     VWDSTKALELAEGAGYAPEEPVRRALFFFLAGQWDAYERLDFSHTLIGTAYENAALPL
                     RRRILSKSRYAGRTEWLQPASEGKPRRWLKDLNDADWDYTLVQLNERLAWDELWRLAL
                     FAPPVWGVQILDKLQQANWQPENPDELDLWQRLGHLSAACRTKAPEIYLQKTLHSPEH
                     SLTALAVSPDGRVMATGCAGQVIQRWQMPRGDRLEPPIIGPVAQTRAMVFSPDGEYLV
                     AASGDQAIRIYRTADGKLVKTLSGHKGTVRGLAIHPDGRTLFSASFDGQVRAWRFPLG
                     PDSQVVFESAGELFALAIAPEGELLITGGANGVLQTIHWPTMQKAHNLAGHKDSVTCL
                     CLSESGQLAASYGRDQTLRLWNPIGGRELRQMVVPGAALTGMCFDPAEQVLMGGGMDG
                     RITFWNTSTGSELLRIERQSKPIIGLALQKSEAVLVTAMADGEILCWGIEAFLLARRP
                     VELSSPTQIQQLEIDLARPNQTTAIRNWLAYLLELMRWKGRYDIQIGETRPIHLGEFD
                     IEL"
     gene            complement(3083..3643)
                     /locus_tag="PKL11_05550"
     CDS             complement(3083..3643)
                     /locus_tag="PKL11_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HNZ13102.1"
                     /translation="MNLRLYHLETHSRANGPGLRAVVWVQGCTLNCPGCFNPETHPIE
                     GGTEWQIDALANALMKVPDIEGITISGGEPLQQIEALTELLQRIRSQSDLSIIVFTGF
                     ERAEMEALPHSEALLVQVDVLITGRYVAKQRQARGLIGSANQEIHCLTNRYTPRDFEN
                     IPEAEIILMPDGEIHLTGIRPPEWQA"
     gene            complement(3683..3889)
                     /locus_tag="PKL11_05555"
     CDS             complement(3683..3889)
                     /locus_tag="PKL11_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HNZ13103.1"
                     /translation="MELQEIEVTIGKNGEVQVHVRGVKGKACLELTAALEAALGGQVT
                     LREMTPEVDETEGLQNDLNLDIKN"
     gene            complement(3899..4270)
                     /locus_tag="PKL11_05560"
     CDS             complement(3899..4270)
                     /locus_tag="PKL11_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HNZ13104.1"
                     /translation="MSHITRIKTKITERDYLVQALKDLGYRFEEGGGITGILRQHAEA
                     DFRVWLTPMFVVGLRHKEDSFEIVADWWGRTKERNTFVNQLNQRYAYVAARAKLEEQG
                     FTLVEEQEEKGQIRLVLRRMA"
     gene            complement(4282..5871)
                     /locus_tag="PKL11_05565"
     CDS             complement(4282..5871)
                     /locus_tag="PKL11_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNZ13105.1"
                     /translation="MSGRLAVDFGTSNTLVAVWDAARKEGVPLQIPDYGLVSLQGGES
                     IAIIPSLIHYAEDKRRWIGNQVLQQGLYESRLTFRWMKRYIAQRSPLKVRLDGREISP
                     FEAGRDFLSSVLVFAAREIDLKDEEVVFSAPVEAYEHYENWLASVAEAAGMPRFRLID
                     EPSAAALGYGAHIQPGHVYLIFDFGGGTMHAAVVLVESDEDAASGRRCRVLGKAGRDL
                     GGSTIDQWLFEETLRQNRRQDSEEAVRRISNALLVECERIKESLSFAEQADLSVLNPD
                     TGAALTATFTRGQFEEILDKRGCLAEINRTVRSALNAARERGYDEDSIQSVLMVGGSS
                     QIPAVQRALRQIFGPERVHSNRPLDAIVRGAAAFAAGVDFYDHIQHDYAIRYINPQTG
                     EYEYRTIVRRGTPYPTPEALTRLVIKASYVGQKQLGLAIFEIGAGRSANTSPIELVFD
                     PGGAARILPISAAEEERRTYFWMNEHTPTFLMAEPPAAAGEERFQVEFRVDGNKRLTI
                     TVRDLKNGTLTHQNFPVVRLT"
     gene            complement(5868..6380)
                     /locus_tag="PKL11_05570"
     CDS             complement(5868..6380)
                     /locus_tag="PKL11_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13106.1"
                     /translation="MNWLKSLLQRRQTQPAEQHDLQNELQTLRLELAEQSWELDALKA
                     GKARDTAAQQERIESEAGARLELIVQEMASAVAQLSVQNYLLIEQGKPVAGADVMAVA
                     QRIVRTLEQHGMEIAPRPGEMMAFDANRHLPLSAGQTIQSGQMVVVRFPGVWFRGKML
                     AKAQVEQKSA"
     gene            complement(6377..9106)
                     /locus_tag="PKL11_05575"
     CDS             complement(6377..9106)
                     /locus_tag="PKL11_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13107.1"
                     /translation="MPKPSLRTRLLSYQIDSTLPLVGAAIRRSAAQGLIRAARGGDGE
                     ATRILAENLAQFPDGEIGQSIRDGLARLAHPGSRDALWAVWKKTRHLELERLAIQIGE
                     PASENSDFETRLLSALKLGQVQFASRIGPSRLPLLLAACRDGDPAIAAAAREAAGKLS
                     NARTIKALCFAWVSERSTLLTDLMAAGKYTTSEPPAVKMLTRLLQNPADLQIDAENVV
                     SLVAALSDPMPEIAARAGYAIRHLQDQPAIDKLCALWEKDRHTDLGDILIQSQYTASA
                     PPELRLLTLLKTGQVELARASHAKWLPLLLEAAHNNDAAIQETARAALQNLKRKDSRE
                     ALCELAVTGEQPLARELAVTAGYRPENPEACARFLFLTGQWEAYENLDFDQRLMRAIY
                     EISSTEIRQKIARQIQTSGRTSYLSILAGVDYRSPTATITEEEAQILIHTLAENQEWE
                     RLWGLTQALALPQAVQIVRLLAKNQWTPASEADAMTYQKLVLLLQNPIALSKEELGAT
                     LSAVFSEELEAILPAALPLAKLKVTGRVNSVAFAPDQPHLAIGVSARKVVRWDYQHGQ
                     IVKLISRFGHSVGQVAFTDDGTLVCGERTNNSSVCSIYAQRRDTLVRLGEHNGSVTGL
                     EGVGAQQAISAGRDGRVILWDTETGQKVAETVLSNRNWARDMAIAPSRNQAVMLYEGI
                     QSVALPSMGLSPSYAPLPIRILGSESRKWESSMLRQAVFTPDEAHLIVGYNNGRVVQC
                     QGLPRRGQKYTYRDLSEFKSPVSGLQFIPGHDVLVMAWDCGNMEFWRNLNPNTRRQSY
                     SPIGRITSLHISPDGAFMATGSDEASLMLWDLRTYDIPEIFQKPLAGVRPGNLPGIMS
                     LAQSKNLSPSVQNALKFLQILLEQRFRYDIQLSEIAQIQPGDYDIIIEEDEEA"
     gene            complement(9119..10657)
                     /locus_tag="PKL11_05580"
     CDS             complement(9119..10657)
                     /locus_tag="PKL11_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ13108.1"
                     /translation="MNFEDQLDIYLRARFTLIVLVTTEEERALTTVKSVCERTQRRAL
                     AWDAAEGFQFITAASGTLPTARDPLTALEQIDKAEDGLFILKDFHEAWGNAQIKRKLR
                     SLGQKLKYTRKSILVTSWTAKIPEELKDEAVIVNFPLPTSPELEDVLVNLTHTPGVKV
                     SLTPLGHEKLVQAALGLTASQAQRVFARAVVREGMLDDRAIDLVTEEKKQIIRESEAL
                     EFYAVTETPDDVGGLGVLKEWLRLRERAFTQEARAYGLPAPKGIALIGIPGTGKSLTA
                     KMIGGLWRLPLLRLDVGALFGSLVGESEERARRALHLAETVAPCIVWIDEMEKALAHG
                     GLDSGTSMRVFGAILTWMQEKTAPCFVVATANDIASLPPELLRKGRFDEIFFLDLPTQ
                     AERREIFSVHLRKRGRIPADFDLEKLAEHTAGYVGSEIEQAIIDAMYIGFNDQRREFT
                     TADILAAVKRQVPLSISQRETIGGLRNWLREGRAQSASFQEIEDAATQFVPIELDIRP
                     GHLG"
     gene            complement(10654..11535)
                     /gene="sdaAA"
                     /locus_tag="PKL11_05585"
     CDS             complement(10654..11535)
                     /gene="sdaAA"
                     /locus_tag="PKL11_05585"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNZ13109.1"
                     /translation="MYRKIEDLVQQAEKLGVPISEIVLRSEIEQNSTTREAVIQQMQH
                     RLEVMRTSTQAGLEAPIHSRSGLTSGSAHRFWGWLEGEGQPITGPVLSRALARALSTA
                     EVNAGMGCIVATPTAGSAGILPAVLVTLQEAYHYPDEALISALFTAAGIGMVIATRAQ
                     IAGAAGGCQAETGSAAAMTAGAVVELRGGSPRQSAQAVAIALKNMLGLVCDPVAGMVE
                     VPCVKRNAAGVGQCFVAVDLAMAGVESVIPADEVIDAMGRIGAHMDERLRETAMGGLA
                     ATPTGKALAQEIWSRKS"
     gene            complement(11538..12218)
                     /gene="sdaAB"
                     /locus_tag="PKL11_05590"
     CDS             complement(11538..12218)
                     /gene="sdaAB"
                     /locus_tag="PKL11_05590"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HNZ13110.1"
                     /translation="MSPAPNSIFDIIGPVMVGPSSSHTAGAVRLGLVARALLGDTPQK
                     AKIELHGSFARTGPGHGTDKALIAGLLGMDTADERIRDSFALAAEAGLAFEFLEADLG
                     EDAHPNSVRFTLATGDKTLEMSGASVGGGMIQITRLQGRSVQIRAELDTLILIADDRP
                     GTINAVSGWLAAHQINIAFLRLKRDQRGGKAMMVIETDQNIPEALTRDLASLPWVHWV
                     YQIPRLKE"
     gene            12393..>13665
                     /locus_tag="PKL11_05595"
     CDS             12393..>13665
                     /locus_tag="PKL11_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ13111.1"
                     /translation="MDLTPEKIQSARTILAELRYETRPVVHGYANRTLYINVGDPQIE
                     IKPVTQQMKDIFTGGRGFCLWLLWNAIKDDTRWDSPENELLIAGGPIGGISQYPGTGK
                     CTAVTISPLTHSIIDSNAGSYFGPYLKFSGFDALEVQGIADQEVIIYIDGDEGIVRVE
                     AAPLDPVDTHLLAPLLTKMYARDEKDQRSISVVTAGQAAAHVPMCGLNVSYYDPRRDE
                     VRIKQAARGGAGTVMRHKHIKAIVVRYSQMSGASNGPANADLIRKAGQRINHEITEFD
                     SSQNDMRGNGTPYLVEIMSRFDLLPIENFRFGSDPRSSQIAGAIWKGMYDRRGPDGCW
                     YGCTMACAHAVPHFHLQTGPYAGQVVFVDGPEYETLGAVGSNLSLWEPAQMLEINFYA
                     DTYGIDSISLGNSIAFAMECYEYGVLNLERTG"
BASE COUNT         2870 a         4297 c         3390 g         3108 t
ORIGIN      
        1 atgatgatag ccgattgaac cgtggcaatg gccgcaatcc aactgtcggt cgcctgaatt
       61 tcccgcacaa agtacagggg aaacagcggc aaagacatgg atatcccggc tgagtaaatg
      121 atccgtttga cgatgaatga gataaatggc ttttcagaaa acaccagcga gagattctgc
      181 agtgggtttg ttttctcacc cggtgatcgc tcaactgatg ggatgataat ctgtcttgaa
      241 aaaataaaac tcaatacccc acccagtgat agcaccaaaa aggcaagctg atagttcacc
      301 gggaataata accggggatt atccagcagt tgcccaatca aaaacaccgt caccgcggta
      361 gtaaagccca gaataatcca ccgccgagac atcaactcaa accgcccgtt aggtccggca
      421 acagcattca tcaccactga aaagcaaatt gccagaacgg tttgcgggat ggtaaccagc
      481 gcccagatag ccaaaattcc ccagaccagc gcctcccctc gcagaaagaa cgctgccagc
      541 cccgatagcg catatccggc aatcaccagc acccgcgcca cgctgtacca gggcaccaca
      601 ttgcgccgcg tctgtaaaaa ctgccccaat gggatggata acagcagacc tgtaatcgct
      661 ggcatggaac tcaataaccc aatctcataa ctgcttgcct gcagacgggc taaaaataca
      721 ggtaaaaacg gcgaagccgc attagccagc ccgacgccaa ccccatcaac ctgcacattg
      781 atgtaattct tacgccgctg tgcatccagg tccagtggcg ccatcaggcg tttgagagtg
      841 gtcttaagat taaagctcaa tgtcaaattc tcccaggtgg atgggacggg tttcaccaat
      901 ttgaatatcg tatcgtccct tccatcgcat caattctaac agataagcca gccagttgcg
      961 gatggcagtt gtttggttcg gtcgggccag atcaatctcc aattgttgaa tctgtgtggg
     1021 tgagcttaat tcaaccggcc tgcgtgccag cagaaaggct tcaatgcccc aacagagtat
     1081 ctcgccatct gccatggccg taaccaacac cgcctccgat ttttgcagcg ccagaccaat
     1141 gatgggctta ctctgccgtt caatacgcaa cagttcgctg ccggtggatg tattccaaaa
     1201 agtgattcgt ccgtccattc cgccacccat cagaacttgc tccgcggggt caaagcacat
     1261 tcccgtcagc gccgctcccg gcacaaccat ttgccgcagt tcccgcccgc cgattggatt
     1321 ccataatcgc agggtctggt cacgcccgta acttgcggcc aactgccccg attcggacag
     1381 gcagagacag gtaacgctgt ctttatgccc tgcaagatta tgggcttttt gcattgtcgg
     1441 ccaatggatg gtttgcagta cgccatttgc cccgccggtt atcagcagtt ccccttccgg
     1501 ggcaatcgcc agtgcgaaga gttctccggc gctctcaaaa accacctggc tgtctggccc
     1561 cagcgggaat cgccaggccc gcacctgccc gtcaaaactg gcgctgaaca gggtccgccc
     1621 atccggatga attgccagcc cgcgcaccgt gcctttgtgc ccgctgagcg tcttgaccag
     1681 ctttccatct gccgttcgat agatgcggat ggcctggtcg ccgctggccg caaccagata
     1741 ttccccgtcc gggctgaaaa ccattgcccg ggtctgcgcc actggcccaa taattggcgg
     1801 ctccagccgg tcaccgcgcg gcatttgcca ccgctgaatc acctgtcccg cacagccagt
     1861 agccatcact cgtccatccg ggctgactgc cagtgccgtc aggctgtgtt cgggagagtg
     1921 cagcgtcttt tgcaggtaaa tttcgggcgc tttagttcgg cacgccgcag ataaatgccc
     1981 caatcgctgc cacaggtcta attcatcggg gttttctggc tgccagtttg cctgctgtaa
     2041 cttgtcaaga atctgcactc cccacactgg cggcgcaaac agcgccagcc gccagagctc
     2101 atcccatgcc aggcgttcat tcaactgcac cagggtatag tcccaatcgg catcgttcaa
     2161 atctttcagc cagcgcctgg gtttaccctc actggcgggt tgcagccatt ctgtccgccc
     2221 tgcgtaccgg ctctttgata gaattcggcg gcgcaatggc agtgccgcgt tctcgtatgc
     2281 cgtgccaatc agtgtatggc tgaaatccag ccgttcataa gcgtcccact gtcccgccag
     2341 gaagaaaaac aaggcccgtc gtacaggttc ttcgggcgca taacccgcac cctctgccag
     2401 ttcaagcgct ttggtgctgt cccatacgag atacagttca cacagcgcct cttttgccgg
     2461 ggcgctccct ctttgaccag tctcatacac tgtatccagc actgcgccct gctcgtctgg
     2521 cgccagagcc ccaaacgcct gaatcagttc agtgtatcgg gcgggggtat cctcaaccag
     2581 cgcagccgcc agcgcatgcc agcccgtcca gccggccttt tgcgctgcct ggagcagccg
     2641 cgcctgcaaa tctggcggcg cgcctgtaaa ttttgcagtc aatattcggt gggttggatc
     2701 cagcctcagg ctttcttttg cctgtcctgt cagcagccag aaaacaatct gggtacccgg
     2761 ctctggcgcg ggtaaattat caacgacaac agccgcctgc gcatcagggt gattgtactg
     2821 aatggcaagt tcaaatactt tagcaatcgc ctcgcctgtt ccactgcgac tcagccgccg
     2881 cagtgattcc agtgcctgag cagcagcctc aacggaaata ccacttcgtg ccacttctgc
     2941 caggccgcca attgcttcag gagtgccggc ttcttctaaa aatgcaacag cgccccgtaa
     3001 agccagccgt tgtgcttctt caatgggcag ttgtgaggct gtcagcaaaa attgaaccag
     3061 gtcgtgcgtt gcatcagaca tatcaggctt gccattccgg tggtctgatg ccggtcaggt
     3121 ggatttctcc atccggcatc aggataattt ctgcttcggg gatgttttca aaatcccgag
     3181 gcgtatatcg gttggtcaaa caatggattt cttgatttgc cgagccaatc aatccccgtg
     3241 cctgtcgctg ctttgccaca taccgcccgg tgataaggac atccacctga accagcagcg
     3301 cctcagagtg cggcagagct tccatttctg cccgttcaaa cccggtgaat acaatgatcg
     3361 acaggtcaga ttgactgcgg atgcgctgca gcaattcggt tagcgcctca atttgctgca
     3421 gaggttcgcc gccgctgata gtgatacctt caatatccgg cactttcatc agggcgtttg
     3481 ccagagcatc aatctgccat tctgttccgc cttctatcgg gtgtgtttct ggattaaaac
     3541 accccgggca gttcagcgta cacccctgca cccagaccac agcccgcaga ccgggcccgt
     3601 ttgcccggct gtgtgtttcg agatgataaa ggcgcaggtt catcaggatt cccaatcatc
     3661 tttctgtgat caaccagtca atctaatttt tgatatccag gttcaggtcg ttttgcaggc
     3721 cttcggtttc gtcaacctcc ggcgtcattt cccgcagagt aacctgcccg cccaatgcag
     3781 cttccagcgc tgcagtcaat tccaggcagg ccttgccctt caccccgcgt acatgcacct
     3841 gcacttcacc gttttttccg atggtcactt caatttcctg tagctccatc atatcctcct
     3901 aagccatccg ccgcagcacc agccggattt gacccttttc ttcctgttct tccaccagcg
     3961 taaagccctg ttcttccagc ttggcgcggg cggccacata cgcatagcgc tggttcagtt
     4021 gattgacaaa ggtattgcgc tctttggtcc ggccccacca gtctgccaca atttcaaagc
     4081 tgtcttcttt atgccgcagg cccaccacaa acatcggcgt cagccacact cgaaagtctg
     4141 cctcggcgtg ctgccgcagg atgcccgtaa taccgccgcc ttcttcaaac cggtagccca
     4201 gatctttgag tgcctgaaca agataatcgc gctctgtaat ttttgtcttt atccgtgtaa
     4261 tatgtgacat aaccttctcc tttaagtcaa tctgaccacc ggaaaattct gatgagtcag
     4321 cgtgccattt ttgaggtcgc gcacggtaat cgtcaaccgt ttgttaccat caacccgaaa
     4381 ttccacctgg aaccgttctt ccccggctgc tgctggtggc tccgccatca agaaggttgg
     4441 cgtgtgttcg ttcatccaga aatacgtccg gcgttcttct tccgccgcgc tgatgggtaa
     4501 gatacgggcc gcgccgccag ggtcgaacac cagctcaatc ggcgatgtgt tcgctgaccg
     4561 tccagcccca atctcaaaaa tcgccagccc tagctgtttt tgacccacat aagaagcctt
     4621 gatcactaaa cgggtcaatg cctcaggggt agggtagggc gtaccccggc gcacaatcgt
     4681 ccgatactca tattctccgg tctgggggtt gatgtatcgg atggcgtaat catgctggat
     4741 atgatcgtag aaatccaccc cggcagcaaa cgccgccgca ccgcgcacaa ttgcatccag
     4801 cgggcggttg gagtgaaccc gctctgggcc aaaaatttgc cgcagcgccc gctgcacagc
     4861 cgggatctga ctgctgccgc cgaccatcag cacagattga atgctgtcct catcataacc
     4921 gcgctcgcgc gccgcattga gtgccgaccg caccgtccgg ttgatttctg ccagacagcc
     4981 gcgcttatcc agaatttctt caaactgccc gcgggtaaac gttgccgtta atgccgcccc
     5041 cgtatccgga tttaatacgg agagatctgc ctgctccgca aaggagaggc tttctttaat
     5101 gcgttcacat tccaccagca aggcattgct gatccggcgc acagcctcct cgctgtcctg
     5161 tcgccgattt tggcgcagcg tttcctcaaa taaccattga tcaatcgtac ttccccccag
     5221 gtctcgcccg gctttaccca gcacccggca gcgccggccg ctggcagcat cttcgtcaga
     5281 ttccaccagc actaccgccg cgtgcatggt tcccccacca aaatcaaaaa tcaaatacac
     5341 atgccccggc tgaatatgcg cgccataccc cagtgccgcc gcagagggtt catcaattaa
     5401 ccggaaccgc ggcattccag cggcctctgc cacgcttgcc agccagtttt cataatgctc
     5461 ataagcttcc acgggcgcac tgaaaaccac ttcctcatct ttcaggtcaa tttctcgcgc
     5521 tgcaaacacc agcacactgg agagaaaatc tcgcccggct tcaaatggcg aaatttcacg
     5581 cccatccagc cgcaccttca gcgggctgcg ctgggcaata tagcgcttca tccaccgaaa
     5641 agtcagccgc gattcgtaga gcccctgctg gagaacctga ttgccaatcc agcggcgctt
     5701 gtcttcggca taatggatca aagacggaat aattgcaatg ctttcgccac cctgcagcga
     5761 aaccaggccg taatcaggaa tctgcaacgg aacgccctct ttgcgcgctg cgtcccacac
     5821 ggcgaccagt gtgtttgatg ttccaaaatc taccgccagg cgaccactca tgctgatttt
     5881 tgctccactt gggctttggc aagcatttta ccgcgaaacc aaacccccgg gaaacggaca
     5941 accaccatct gccctgattg aatggtttgc cctgcgctca aaggcaggtg ccgattagca
     6001 tcaaacgcca tcatttcacc tggtcggggc gcaatctcca tgccatgctg ctccagcgtt
     6061 cgaacaatcc gctgcgctac cgccatcaca tccgcccccg caaccggttt cccctgttct
     6121 attaggagat aattctgtac gctgagttgc gccacggcgc tcgccatttc ctgaacaatc
     6181 aattccagcc gcgccccggc ttcagattca atccgctctt gctgagcagc cgtatcgcgt
     6241 gctttgcctg ccttaagcgc atccagttcc cagctttgtt cggccagctc cagccgcagg
     6301 gtttgaagtt cgttttgtaa gtcgtgttgt tccgctggtt gggtttgtct gcgctgcagg
     6361 agagatttta accagttcat gcttcttcat cctcttcaat gataatatca taatcccccg
     6421 gctgtatttg tgctatctcc gagagttgaa tatcatacct gaagcgctgc tccagcaaaa
     6481 tttgcaggaa cttcaacgcg ttttgcacgc ttggcgaaag gttttttgac tgcgccagtg
     6541 acatgatccc tggcaggttg cccggccgca cccccgccag gggtttctgg aagatttccg
     6601 gaatatcgta agtacgcaaa tcccagagca tcagactggc ttcatcgctg cccgttgcca
     6661 taaatgcccc gtcgggtgaa atatgcagcg atgtgattcg cccaatcggc gaataggatt
     6721 ggcggcgcgt gtttggattc agatttcgcc aaaattccat attcccacag tcccacgcca
     6781 tcaccagcac atcatgcccg ggaatgaact gcagtccact gaccggtgat ttgaactcgc
     6841 tcaaatcccg atatgtgtac ttctgcccgc gccgcggcag cccctggcat tgcaccaccc
     6901 gcccgttgtt gtaccccaca atcaagtgcg cttcatccgg cgtgaacaca gcctggcgca
     6961 gcatgctgct ttcccatttt cgcgattcac ttcccagtat tcgaataggc agcggggcgt
     7021 aagatggcga aagtcccata ctgggcagcg cgacagattg aatcccctca tagagcataa
     7081 cagcctgatt ccggcttgga gcaatcgcca tgtcccgggc ccagttgcgg ttactcaaca
     7141 cggtttcagc aactttctgc cctgtttcgg tatcccacag gataacccgc ccgtcccggc
     7201 ccgcagagat cgcttgctgc gcgccaacgc cttccagccc agtgaccgaa ccattgtgct
     7261 cacccagtct caccaaggtg tccctgcgtt gggcataaat cgagcaaacg ctagaattgt
     7321 tggtccgctc gccgcaaacc agtgtgccgt catctgtaaa cgcaacctgc cccaccgaat
     7381 gcccaaacct gctgatcagc ttgacgatct gcccatgctg ataatcccag cgcaccacct
     7441 tgcgggcgct caccccaatt gccaggtgcg gttgatccgg ggcaaacgcc accgaattta
     7501 cccgcccggt taccttcagt tttgccagcg gcagcgccgc cggcaggatt gcctccagtt
     7561 cctctgaaaa aacggccgat aaggttgccc ctagctcctc tttcgataga gcaattgggt
     7621 tttgaagcag caaaaccaat ttttgatagg tcattgcatc cgcttcactg gcaggagtcc
     7681 actgattctt tgccagcagc cgcacaattt gaaccgcctg cggcagcgcc agcgcctgcg
     7741 tcaatcccca cagtcgctcc cattcttgat tttcggccag cgtatggata aggatttgcg
     7801 cttcctcttc agtaatggtg gcagtagggg agcggtaatc aaccccggcc aaaatcgata
     7861 gataactcgt tcgcccggag gtttgtatct gccgggcgat tttctgccga atctccgtgc
     7921 tgctgatttc ataaatggcc cgcatcagcc gctggtcaaa atccaggttt tcgtaagcct
     7981 cccattgccc ggtcagaaag agaaatcgcg cacaggcttc cggattctct ggacggtatc
     8041 ccgccgtaac ggccagctct cttgccagtg gctgctcgcc agtaaccgcc agttcgcaaa
     8101 gcgcttcgcg gctgtctttc ctcttgaggt tttgaagtgc tgcccgggct gtctcctgga
     8161 tggccgcatc gttattgtgg gctgcttcca acagcaaggg cagccattta gcatggctgg
     8221 ctcgcgccag ttcgacctgt ccggttttga gaagcgtcaa taaccgcagt tcaggcggag
     8281 ccgatgccgt gtactgcgat tgaatcagaa tatcccccag gtcagtatgg cgatcttttt
     8341 cccataatgc gcatagctta tcaatggcgg gctgatcttg taaatgccgg attgcataac
     8401 cggctcgtgc tgcaatttct ggcatcggat ctgacaatgc tgccaccaat gaaaccacgt
     8461 tttcagcatc aatttgtaaa tctgccgggt tttgcagcag tcgggtcagc atcttcactg
     8521 ccgggggctc gctggtagtg tacttcccgg cagccattag atcggtcagc agcgtgctgc
     8581 gttcactgac ccaggcaaag cacagtgcct tgattgtccg ggcgttgctc aatttcccgg
     8641 cagcctcacg cgccgcagcg gcaatcgccg ggtcgccatc ccggcaggca gccagcagca
     8701 aaggcaggcg gctgggccca atccgagatg caaattgaac ctgcccaagt tttaacgcac
     8761 tcagcaatcg cgtctcaaaa tcgctgtttt ctgatgctgg ttcgccaatc tgaatcgcca
     8821 ggcgttcaag ctccagatgc cgcgttttct tccagaccgc ccagagcgca tcccgcgatc
     8881 ctggatgggc cagtcgcgcc aacccatccc gaatgctttg accaatttcc ccatcgggga
     8941 attgcgccag gttttctgcc agaatccggg tcgcttcacc atccccgccc cgcgccgccc
     9001 gaatcaatcc ttgtgctgcg ctgcgtcgta ttgcagcacc caccagcggg agggtgctgt
     9061 caatctggta gctcagcagg cgggtgcgca ggctgggttt cggcatggca tcgccgaact
     9121 agcccagatg tcctggtcga atatccagtt caatgggcac aaattgcgtg gccgcatctt
     9181 caatttcctg gaaggatgcc gactgtgccc gaccttctcg cagccagttg cgcaatccgc
     9241 cgattgtctc gcgctgtgaa attgaaagcg gcacctgccg tttcaccgcc gccagaatat
     9301 ccgcagtggt aaattctcgc cgctggtcat tgaagccaat atacatggcg tcaataatgg
     9361 cctgctcaat ttccgagccc acatacccgg ctgtgtgctc tgccagtttc tccaggtcaa
     9421 aatctgccgg aatgcgcccg cgtttgcgca gatgcacact gaaaatttct cgacgctcgg
     9481 cctgtgttgg cagatcgagg aagaaaatct catcaaaccg tcccttgcgc agcagttctg
     9541 gcggcaggct ggcaatgtca ttggcagtcg ccaccacaaa gcacggtgca gtcttttcct
     9601 gcatccaggt cagaatggcc ccaaagacgc gcatactggt gccgctgtcc agcccgccgt
     9661 gtgccagcgc tttctccatc tcatcaatcc acacaataca cggcgccacg gtttcagcca
     9721 gatgcagcgc ccgccgcgcc cgctcttcag attcacccac caggctgcca aacaaggccc
     9781 ccacatccag tcgcagcagg ggcagccgcc acagcccgcc aatcatcttt gctgtcagac
     9841 tctttcccgt gcccgggatc cctatcagag caatcccctt gggggccggc agcccgtaag
     9901 cccgggcttc ctgagtgaag gcgcgctctc gcagccgcag ccattcttta agcactccca
     9961 gcccgcccac gtcatccggc gtctcggtga ccgcataaaa ttccagcgcc tcgctctcgc
    10021 gaataatctg ctttttttcc tcggtcacca ggtcaatcgc ccggtcatcc agcatgcctt
    10081 ctcgcaccac ggcccgggca aagacccgct gcgcctgtga agccgtcagg cccagcgcag
    10141 cctggactaa cttctcgtgt cccagcggcg tcaggcttac cttgaccccc ggcgtatggg
    10201 tcaggttcac cagcacatct tccagctctg gggaggtcgg cagcggaaaa ttgacaatca
    10261 ccgcctcgtc tttcaattct tccggtatct tggccgtcca ggaggtcacc aggatggatt
    10321 tacgggtgta tttgagcttc tgccccagcg agcgcaattt gcgtttaatt tgcgcgtttc
    10381 cccaggcctc gtgaaaatct ttcaaaatga acaatccatc ttcagcctta tcaatctgct
    10441 ccagggccgt taacggatct cgggcagtgg gcagcgtccc gctggcagcc gtgataaatt
    10501 gaaacccctc ggctgcgtcc caggccagcg cccgccgttg ggtgcgctcg cacactgatt
    10561 taactgttgt tagggcgcgt tcttcctcag tcgtcaccag cacaatcagg gtgaaccggg
    10621 cgcgcagata gatgtccagt tgatcttcaa agttcatgat tttcggctcc aaatttcctg
    10681 tgccagggcc tttcccgttg gggtggccgc cagaccgccc atagcggttt cgcgcaaacg
    10741 ctcatccatg tgagcgccaa tccgtcccat cgcgtcaatc acttcatccg ccgggatcac
    10801 actctccacc ccggccatcg ccaggtccac agccacaaaa cactgcccca caccagccgc
    10861 attgcgcttc acgcagggca cttccaccat tcccgccact gggtcgcaga ccagccccag
    10921 catatttttc aaggcaattg ccaccgcctg ggcagactgc cgcgggctgc cgccccgcag
    10981 ttccaccacc gcaccggccg tcattgccgc tgcgctgccc gtttctgcct gacacccccc
    11041 cgccgcgcca gcaatttgcg cccgggtggc aatcaccatg ccaattccag ccgccgtgaa
    11101 caaggctgaa atcagggctt cgtcagggta gtgataggct tcctggagcg tgaccagcac
    11161 cgctggcaag atccccgccg agccagccgt tggggttgcc acaatgcagc ccatccccgc
    11221 attgacctca gccgtagaca gcgctcgcgc cagcgcccgg ctcagcaccg gcccggtaat
    11281 gggctgtccc tcgccttcca gccagcccca aaaacgatgg gcgctgccgc tggtcaatcc
    11341 actccttgag tgaatcggcg cctccaaccc tgcctgggtt gacgttcgca tgacctccag
    11401 ccgatgctgc atttgctgga tcacggcttc gcgcgtggta ctgttctgct caatttcgct
    11461 tcgcagcaca atctcagaaa ttggcacgcc cagtttttct gcttgttgaa ccagatcttc
    11521 aattttgcgg tacatggcta ctctttaagg cgcggaattt gatataccca gtgcacccac
    11581 ggcagcgatg ccagatcccg cgtgagtgcc tctggaatgt tttgatcagt ctcgatgacc
    11641 atcatggctt tgcccccgcg ctgatcgcgc ttcaagcgca ggaaagcaat attgatctga
    11701 tgtgcggcca gccacccact cactgcattg atggtgccgg ggcgatcatc ggcaatcaaa
    11761 atcagcgtat ccagctcagc ccgaatttgt accgagcgcc cctgcagtcg ggtgatttgg
    11821 atcatcccgc cgcccaccga agctccgctc atttccaggg ttttatcgcc cgtcgcgagc
    11881 gtgaatcgca ctgaattggg gtgtgcgtct tctcccaggt ctgcttccag aaattcaaag
    11941 gccagccctg cttctgcggc cagcgcaaaa ctgtctcgga tccgctcatc cgccgtatcc
    12001 atgcccagca gcccggcaat cagggcttta tccgtgccgt gcccgggtcc ggtgcgcgca
    12061 aacgaaccat gtagttctat tttggctttt tgcggtgtgt cacccagcaa ggcccgcgcc
    12121 accagcccta gccgcaccgc ccctgcggta tgcgaactgg acggcccgac catcaccgga
    12181 ccaataatat caaaaatgga gtttggcgct ggactcatga ttcgaaatta tagcacgaat
    12241 gatgacattc atctgacgaa tgaatgacgc cgcgcagccc ggattgggac tgcgaaaaat
    12301 tataatatta tcggaagttc gttatatctg atgtccctta tctcaataac cctttttggc
    12361 tggcagggct gacctgcaaa tggagcaatg tgatggatct gacccctgaa aaaatccaat
    12421 cggcccgcac aatcctggct gagctgcgtt atgaaacccg cccggtggtt cacggctatg
    12481 ctaatcgcac gctctatatt aacgttggtg acccgcagat tgaaatcaag ccggttaccc
    12541 agcagatgaa agatattttc accggcgggc gcggcttttg cctttggctc ctctggaatg
    12601 cgatcaaaga tgacacccgt tgggattctc ccgaaaatga gctgctgatt gcgggcggtc
    12661 ccatcggcgg catcagccag taccccggta ctggtaagtg cacagcagtc acaatttccc
    12721 cgctgaccca ttcgattatt gacagcaacg ccggcagcta ctttggcccg tacctcaaat
    12781 tctctggctt tgacgcgctt gaagttcagg gcattgccga ccaggaagta atcatctata
    12841 ttgatggtga tgaaggtatt gtccgggttg aagctgcgcc tctcgatccc gtagataccc
    12901 acctgctggc accgcttctg accaaaatgt atgcccgcga tgaaaaagac cagcgctcca
    12961 tttcagtggt caccgccggg caggcagccg ctcatgttcc tatgtgcggc cttaacgtca
    13021 gctactacga cccacggcga gatgaagttc gcatcaaaca ggcggctcgc ggcggcgccg
    13081 gaacggtaat gcgccacaaa cacatcaagg ccatcgttgt ccgttacagc cagatgagcg
    13141 gcgccagcaa cggcccagcc aatgcggatt tgatccgtaa agccggtcag cgcattaatc
    13201 acgaaatcac cgagtttgac tcctcacaga atgatatgcg cggtaatggt acgccttacc
    13261 tggtcgaaat tatgagccga tttgatttgc tgcccatcga gaacttccgt tttggctctg
    13321 acccgcgttc ctcacaaatt gctggcgcta tctggaaagg tatgtatgac cgccgcggcc
    13381 ccgatggctg ctggtacggc tgcaccatgg cctgtgcgca cgctgtcccg cacttccacc
    13441 ttcaaaccgg tccctatgcc ggtcaggtgg tctttgttga tggtccggaa tatgaaaccc
    13501 ttggcgccgt tggctccaac ctgtcacttt gggagcccgc ccagatgctc gaaatcaact
    13561 tctatgccga tacctatggt atcgactcga tttccctggg taactcaatc gcctttgcca
    13621 tggagtgtta tgagtatggc gtcttgaatc tcgagcgcac cggcg
//
LOCUS       DAOPVS010000046        13603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4614_length_13603_cov_53.758636, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000046 DAOPVS010000000
VERSION     DAOPVS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13603
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_13603_cov_53.758636"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1848
                     /locus_tag="PKL11_05600"
     CDS             184..1848
                     /locus_tag="PKL11_05600"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNZ13112.1"
                     /translation="MKRRLFAAILLLSVLAACTPTLPAQPTPIPSPLPPTSTPSSQPS
                     ATLLPSPTPLPALSVYIAPNVPPALLDQLQVAPDVQRVSDPTSANLRLTIWPDTWNQS
                     TWVYALAAPFPTLVDGVSLSELQSAWRGQSVKVFAGQPLWMTPQTLDAFSAQWGAPAE
                     SAVQVFPADELLDRAWTAGTAWVILPFEDLGPRWKVLRVADQSPYDQAFNPSTYPLTV
                     YYGLYGEEALITALKALPVPPVSLPTTNRDPDKFTALILTGTTALVRRTALRMEEEGV
                     LYPAVQIGDYLRSADLLHISNEISFDPECPPARPLYTGSRFCSSPAYWELFESLDVDL
                     FELTGNHMLDWKAPPFLATLALYQEQGIPYYGGGANLTEAQRPLLLEDHGNRLAFIGC
                     NAAGPAIALAGEDNPGAAPCEDYDWLADQVTALKSQGYLVIVTFQAIEVDDYEPSSAQ
                     TVNFGRIARLGPVIVSGSQSHFPQGMAFEGDTFIHYGLGNLFFDQMDRDHRRAFIDRH
                     IFYDGRYLGVELATTMMEDYAQPRWMTPEERTKLLGFAFAASDWAK"
     assembly_gap    1985..2084
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2085..3221
                     /locus_tag="PKL11_05605"
     CDS             <2085..3221
                     /locus_tag="PKL11_05605"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNZ13113.1"
                     /translation="AWENRPSWAILPFEDLNPAWKVLHVDAQSPYDPAFYPAAYPLAV
                     NFSLTVSQRAAQTYAGLGLAVPALPASNRDPQKFTTVVITGTTAIVRTLAARIDEKGA
                     DYPAALIRDWLVSADITHISNESSFTPDCPAARWTSTTRMFCSTPAHVALFHDVGADV
                     IELSGNHLGDYGADPIVFTLDLLADEGFLTYASGLNQESARQGITLEHHGNRIAFIGC
                     NEAGPDYVWATETRPGAAQCDMDWLAAEVQRLKAEGYLPVVTFQYRESQGTHPMPWEI
                     RDFRRMVDSGAVIVSGSQSHVPMTMEFYNGGFIHYGLGNLFFDQMTADKYRQEVIDRH
                     IFYDGRYLGSDLLTAYLEDYSQPRPMTATERADLLTRIFHDSGW"
     gene            3493..3849
                     /locus_tag="PKL11_05610"
     CDS             3493..3849
                     /locus_tag="PKL11_05610"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNZ13114.1"
                     /translation="MTLDAFFQGYESSRPIFDALRAVLDTIGPTELRVSKSQIAFWRR
                     RAVARVWIPARYLRGNPAPLVLTLAFRQPIPSPRWKQIAHPTPAWTIHHLELSTAAEI
                     DAEVACWLRLAWEQAA"
     gene            complement(3923..5269)
                     /locus_tag="PKL11_05615"
     CDS             complement(3923..5269)
                     /locus_tag="PKL11_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13115.1"
                     /translation="MSKKIRLILPLMALMLAMLACGLPAIPTPPPGDIVETNVAATVG
                     SFFADTTPTPAATPTEPVPTITAAPSEVPTLPTPAERQVVFVQTGNLWLWRASSGAVQ
                     LTSDGGAVEARLSPDGQKVAYSKWMTEFNKELWLINRDGSGNRMLLSAVDIANLDAEK
                     ALSLYNFWWLPDSQKIGYETQFAVEIGVPRSWDFQVIDTVTGEGGTLVPAGQSGNVLA
                     SPDGQKLAVTTATNIRVIELSTGIHRMLLSYPFVYTYSEWAYVPTPVWAPDSESLGVI
                     IPPQDSLGVETPIGTLWIAPLHAAPYEAASFDLSPVFISDTSVAPNLNQYIYGTGANP
                     DTGQVHLGQVAPPSSQVIINGSVRYSGWSADSAHYFFTQSESDGGDTLYVAAPGSSPQ
                     VLAGNVHAYTVRWVGNDQVIFISGTYGSAELHLMKLSGSDTIIAVGGDDLTFDTTR"
     gene            5438..6070
                     /locus_tag="PKL11_05620"
     CDS             5438..6070
                     /locus_tag="PKL11_05620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNZ13116.1"
                     /translation="MKIYDVSLTITPSLPVWPGDPQVDLHRVSKMEDGAVCNISHLTL
                     GVHTGTHVDAPYHFLPNGGRVDALPLEALIGPALVLEVPETCTAIGAAELRAAGLPGR
                     VERVLFKSRNSNLWSSEKEFYREFVAILPDAAEMLVAAGVRLVGMDYLSVAPFNNAVR
                     THEILLSAGVVLLEGLNLAEVPAGLYTLYCLPLKLGGADGAPARTVLIGA"
     gene            complement(6057..6827)
                     /gene="fabL"
                     /locus_tag="PKL11_05625"
     CDS             complement(6057..6827)
                     /gene="fabL"
                     /locus_tag="PKL11_05625"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNZ13117.1"
                     /translation="MLPLENKIGLVTGSSRGIGRAIALRLAQDGADVVVTFVRNEGPA
                     RQVAAEIEGLGRRAVVVQANLSKLEDIERLFDTIVSEFGGLDIFISNAASGFNRPAMQ
                     QKPQGWDYTLNVNARAFLFAAQRAAPLVQSRGGGQMVAISSPGSSRVLPDYVAVGASK
                     AALEALTRYLAVELAPANIRVNAVAPGLVLTDALQHFATLGDPAVIERAMKNTPAGRL
                     VTPEDVAGVVAFLCSPAAAMICGQVIVLDGGYTLITPQ"
     gene            complement(6832..7248)
                     /locus_tag="PKL11_05630"
     CDS             complement(6832..7248)
                     /locus_tag="PKL11_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNZ13118.1"
                     /translation="MVGQDLEANLIEGCGEALPRLAQRGMEHFNQGEYFEAHEWLELA
                     WREEKRPIRELYRGILQVGVAYHHLRRGNIRGAIRLLERAEGWLAQFPDVCQGVDVRR
                     LRQDADRVRQVLEENEATGATVRIDLRPVSFQQTKE"
     gene            7385..8734
                     /locus_tag="PKL11_05635"
     CDS             7385..8734
                     /locus_tag="PKL11_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ13119.1"
                     /translation="MIDLPDDQILPLSLEHNFWTWSAQGKVSPIPVKRSQGVYFWDVN
                     DKRYLDFNSMVMCVNIGHGDQRVIQAIINQAQELPFAGPGMATRPRARLGQLLAEITP
                     PGLTRFLYTLGGADANENAIKLARAFTGRSKILTRYRSYHGATYGAIAATGDPRRAAW
                     EPAVMPGVVHFLDPYRYRSTFHRTNPNISDEDFTRDYLNHLEEIILYERPETIAAVMV
                     ESVTGTNGIIIPPPGYMQGLRELCNKYDILLICDEVMSGFGRTGEWFAVNHWNVNPDI
                     MTMAKGLTSGYAPLGAVAMRQEIADTFKDKVYEGGLTYNGHPLSLAAAIAVIEAMQAD
                     HMVEHARQMGEVLSDMLQELVDRHPSVGEVRSIGLFGILEIVRNRQTREPMAPFGGSS
                     PEMNAFKKYMSDHGVFLYTHWHTVLIIPPLIITPEELAEGFAILDQALEITDAAVKS"
     gene            8747..9718
                     /gene="trxB"
                     /locus_tag="PKL11_05640"
     CDS             8747..9718
                     /gene="trxB"
                     /locus_tag="PKL11_05640"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNZ13120.1"
                     /translation="MEFNLTPLAQASSSTEPQSEKVLILGAGPAGLAAALYAARAELS
                     PLVLTGLSIGGQAAATHLIENYPGFPEGIGGAELGEQFRLQAERFGARFDFDTATRVD
                     LSQRPFRVYTYDREFHAETLILATGASPNHLNIPGEKELTGKGVSYCGTCDGWFFKGK
                     RVAVVGGGDSAIEEAIFLTRYAETVTIIHRRDALRAGAILQNRARQNPKINFIWDAVV
                     TAAEGQGALANLKLRNVKTNAESNFTTDGLFIFIGNTPNTDMFRGQLDMDDKGYVVTN
                     ARMETSLPGVYAAGEVTDSVFRQVITSAGMGAAAAIQAIRFLEEHDA"
     gene            complement(9767..10759)
                     /locus_tag="PKL11_05645"
     CDS             complement(9767..10759)
                     /locus_tag="PKL11_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ13121.1"
                     /translation="MPNISLSLPIALLIMLVLLGIGAGVVFAVLRGTGRVVEPTATAT
                     LTITPTVTLTPTATSTATQVPTPTPLPPIEYVVKSGDNCGTIALTFNVSIASIIVSNN
                     LDSNCTTLVPGQTLRIPQPTPTWTPPPTATLSAVEATDAACQKISYTVTANDTLSGIA
                     DMYNVSIQSIKDYNGLPSDSVWEGQKLDIPLCERLPTPGPTPTPTPPPPYPAVSLLLP
                     ADGSAFMNANDVITLQWAAVSTLLQNEAYAVTIEDVTSGDRRLVEYVTDTKFIVPANF
                     RPADSLPHIMRWYVTIVRQTGTTNDDQPIYSPAGAISTPRVFTWWSSGVTTPNP"
     gene            10966..12237
                     /gene="hemW"
                     /locus_tag="PKL11_05650"
     CDS             10966..12237
                     /gene="hemW"
                     /locus_tag="PKL11_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNZ13122.1"
                     /translation="MKIGQVPYASAPIRDILTLMQVPLSLYVHIPFCRRRCGYCDFNT
                     YAGMESWIAPYSAALKQEITEVALATGARLPVGTVFWGGGTPSLLPIEQIALIQNAYS
                     QYYALLSDVEITLEANPGTVSLSWLTELRRLGFNRLSFGMQSANPDELHLLDRAHAPR
                     DVQSAVAWSRQAGFDNLNLDLIYGLPNQRLSDWQNTLSAALQLEPEHLSLYSLIVEPG
                     TPLAAQVEAGRLPPPDDDLAADMMDLAAEVLSAAGFVQYEISNFARGLEHSCRHNLQY
                     WRNLPYLGFGAGAHGSAAGWRTANLASISAYLNALTASSEWPAYPFSPANAERNPIDR
                     WTEVQETLMVGLRLTHEGVSLKGFADRFGMDLMALFGATIDRLQAQGLLEWAELEFGK
                     ALRLTSHGRLLGNQVFAAFIGDPEPPLFHNH"
     gene            complement(12220..13260)
                     /locus_tag="PKL11_05655"
     CDS             complement(12220..13260)
                     /locus_tag="PKL11_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNZ13123.1"
                     /translation="MRTKISRRDFLKIAGLSMAALAFRPYYEHGEAVDDGALARVAVY
                     SVSVHKQPSDDSQIVHQRYRDELLHVYEEVVSPDGPGWNPVWYRVWGGYVHSGYLQKV
                     QTRFNPVASKLKAERQLARVTVPFSQAMFYTEVAGWQQVYRLYYDSVHWVTGIDEGPD
                     GAPWYKVWDELLNLKYFVPAPHLSMINAAEYAPITPDVPHGKKWIHVSISRQELTAYE
                     NDQVVLLTKCSTGLPSRNLPPGAIPTETPKGSFHIQNKMPVRHMGDGNLTADPEAYEL
                     PGVPWVSYFEPETGVAIHGTYWHQNYGVQMSHGCVNLKPEEALWVFRWATPAYVEGDV
                     YRVGYGTSVIVE"
     gene            13341..>13603
                     /locus_tag="PKL11_05660"
     CDS             13341..>13603
                     /locus_tag="PKL11_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13124.1"
                     /translation="MINHKLLAWTQKGLFVLVWALSSSLGWWVIISINALTSLSAGLY
                     DPDEVVLYLSQSPALRAFNVLLPALVGGLIFAVIVGALQWYSGG"
BASE COUNT         2710 a         4369 c         3385 g         3039 t
ORIGIN      
        1 cttcctggcc tggttgatgg ggcgcagcat gctggagacg aaagggatgc tttcaccctg
       61 gattatccac ttcatccccg atgtcgttgt cttcatatcc tacgcacttc tctttgtcca
      121 atcctgattc atttcataaa cctgcttaat ccatccggat gaggatggat tataatctcc
      181 gccatgaaac gccgcctctt tgcagccatt cttttattat cggttctggc tgcctgcacg
      241 ccgaccctgc cagcccaacc cacgcccatc cccagcccgc tccctcccac gagcacgccc
      301 tcgtcgcagc cttcggcaac ccttctgcct tcacccacgc cgctccccgc cctcagtgtg
      361 tatatcgctc cgaatgtccc gcctgcgctt ctggaccaac ttcaagttgc gcccgatgtg
      421 cagcgcgtct ctgacccaac ctcggccaac ctccgtctga ccatctggcc cgatacctgg
      481 aaccagtcga cctgggtcta tgccctggcc gcgccgttcc ccaccctggt ggatggcgtc
      541 agcctgtcgg aactgcagtc tgcctggcgc ggccaatccg tcaaggtctt tgccggtcag
      601 ccgctctgga tgacgcccca gaccctggac gcattctcgg cccagtgggg cgccccggcc
      661 gagtctgctg tgcaggtctt cccggcagat gaactgcttg accgggcctg gacggctggc
      721 actgcctggg tcattctgcc ctttgaagac ctcggaccgc gctggaaggt tctgcgcgtg
      781 gccgatcaga gtccctacga ccaggctttt aatccttcca cctacccgct cacggtctat
      841 tatggcctct atggcgaaga agcgctcatc actgcgctca aagccctgcc cgtgccgccg
      901 gtcagcctgc ccaccaccaa ccgtgatcct gataaattca ccgctctaat cctcaccggc
      961 accaccgccc ttgtccgccg cactgccctg cgcatggagg aagagggtgt cctctacccg
     1021 gccgttcaaa tcggcgatta tctccgctct gccgatttac tccatatcag caacgaaatc
     1081 tccttcgatc ccgaatgtcc ccccgccagg ccgctttata ccggctctcg cttctgttcc
     1141 tcaccagcct actgggaatt attcgaatcg ctggatgtgg acctgtttga actcaccggc
     1201 aaccatatgc tcgattggaa agctccgcct ttcctcgcca cgctggcgct ttaccaggag
     1261 caggggattc cttattatgg tgggggcgcc aacctgaccg aggctcagcg cccgctgctt
     1321 cttgaagatc atggcaaccg cctggccttc attggctgca atgctgccgg ccccgccatt
     1381 gccctcgccg gtgaagacaa ccccggcgcc gctccctgcg aggattacga ctggcttgcc
     1441 gaccaggtga ccgctctcaa atcccagggc tatctggtaa ttgtcacctt ccaggccatc
     1501 gaggtggatg actacgaacc ttcctctgcc cagaccgtga attttggccg tattgcccgg
     1561 ctgggcccgg tcatcgtttc tggcagccag tcgcatttcc cccagggcat ggcttttgag
     1621 ggcgacacct tcattcatta tggccttggc aacctcttct ttgaccagat ggatcgtgac
     1681 caccgccgcg ccttcattga ccgccatatc ttttacgatg gccgctacct gggcgttgag
     1741 cttgccacta ccatgatgga agattatgcc cagccgcgct ggatgacccc tgaagaacgt
     1801 accaaactcc tcggctttgc ctttgccgcc agcgattggg caaaatgaac ccgcttctgc
     1861 acccctggta aatgatagga gctgtcccca cttcttatga agccatttcg gatcatcctg
     1921 tttctcatcc tgctgaccgc ctgcacgccc gccccaaccc ctgtggtgct gccttcgcca
     1981 accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcctgg gaaaaccgcc
     2101 cttcctgggc catcctgccc tttgaagacc tcaaccccgc ctggaaagtt ctccatgtag
     2161 atgcccaatc gccctatgat cccgcctttt accctgcggc atatcccctt gcggtgaact
     2221 tctccctgac cgtcagccaa cgcgccgcgc aaacttatgc cggcctgggg ctggccgtgc
     2281 ctgccctgcc tgcatccaac cgtgaccccc aaaaattcac cacagtcgtc atcaccggca
     2341 ccaccgccat cgtccgcacc ctggctgccc gcattgatga gaagggtgcc gactatcccg
     2401 ccgccctgat tcgcgactgg ctggtgtctg ccgatatcac ccacatcagc aacgaatcat
     2461 ccttcacccc cgattgcccc gccgcccgct ggaccagcac cacccgtatg ttttgcagca
     2521 ccccggcgca tgtcgccctc ttccacgatg tcggcgccga tgtgattgag ctttcgggca
     2581 atcatctggg cgactatggc gcagacccca tcgtcttcac cctggattta cttgccgacg
     2641 aaggcttcct gacctacgct tccggcttga accaggaatc cgcccgtcag ggtatcaccc
     2701 tcgagcatca cggcaaccgc attgccttca tcggctgtaa cgaagccggc cccgattatg
     2761 tctgggcaac ggaaacacgc cccggcgccg cccagtgcga tatggattgg ctcgcagccg
     2821 aagttcagcg ccttaaggca gagggctacc tcccggttgt caccttccag taccgtgaat
     2881 cgcagggcac ccaccccatg ccctgggaaa tccgtgattt tcgccgtatg gtcgattctg
     2941 gagctgtaat tgtcagcggc agccagtctc acgtccccat gaccatggaa ttttacaacg
     3001 gcggatttat ccattacggc ctcggcaacc tgttcttcga ccagatgact gccgataaat
     3061 accgtcagga agtcattgac cgccacatct tctatgatgg ccgctacctg ggcagcgatc
     3121 tgctcaccgc ctatcttgaa gactattccc agccccgtcc catgaccgcc accgaacgtg
     3181 ctgatcttct cacccgcatt tttcatgact caggctggta attgcgctat aatcagagta
     3241 tctgaggcag catagtctca cccttcgaac aataatcata aaaggagacc cccgatgggc
     3301 gacaaaggcg gcaagaaaga aaaagaaaaa gccaaaaagc agaagcagga tcaggttcaa
     3361 aagaagcagg aacagcagaa gaacaagcag cctgctaaga agcctgcctg atccaaattc
     3421 attgcatcaa aaatcacccc ccatcccaat catagggatg ggggcgcttg tatctgctat
     3481 tgagaatatg gcatgacgct ggatgcattc ttccagggct atgagtcttc ccgcccgatt
     3541 ttcgacgctc tccgggccgt attggatacc attggcccca cagaactgcg ggtctccaaa
     3601 agccaaatcg ccttctggcg gcggagagct gttgcccgcg tctggattcc cgcccgttat
     3661 ctgcggggaa atccggctcc cctggtgttg actctggcct ttcgccaacc catcccctcg
     3721 ccgcgttgga aacaaattgc ccaccccacc cccgcctgga ctatccacca tctggaactt
     3781 tccactgcag ccgagattga cgctgaagtg gcctgctggc tccggctggc ctgggagcaa
     3841 gccgcctgac ccataggtgt tattcaaaca aaagagcgag gcggttatcc gcctcgctct
     3901 tatttatcct cacacgcacc ctttaccggg ttgtgtcaaa tgtcaaatca tcacccccca
     3961 cagcgataat cgtgtcgctc ccgctgagtt tcatcaggtg cagttcagcg ctgccatacg
     4021 tcccactgat aaagataacc tggtcattgc ccacccaacg caccgtgtag gcgtgaacat
     4081 tgcctgccag cacctggggt gagctgcccg gcgcagcaac atagagtgtg tctccgccat
     4141 cgctttctga ctgtgtaaag aagtaatgcg ccgaatcagc actccaaccg ctgtaccgaa
     4201 cgcttccgtt gataatcacc tggcttgaag gtggtgccac ctgccccagg tgaacttgac
     4261 ccgtatccgg gttggcgccg gtgccataaa tatactgatt caggttcgga gcaactgagg
     4321 tatccgaaat gaaaactggt gacagatcaa aacttgcagc ttcgtacggc gccgcgtgaa
     4381 gcggagcaat ccacagtgtt ccaatcggcg tctcaacgcc cagtgaatcc tgcggtggga
     4441 taatcacccc cagcgattcg ctgtccggcg cccacacagg tgttggcaca taagcccact
     4501 cactgtaggt gtacacaaac gggtagctta acaacatccg gtggatcccc gtggaaagct
     4561 caatgactcg aatattggtc gccgtggtga ccgccagttt ctgcccgtct ggcgatgcca
     4621 gcacgttgcc gctttgcccg gcgggaacca gtgtgccgcc ttctcccgtc accgtgtcaa
     4681 tgacctgaaa atcccaactg cgcggcacgc caatctcaac ggcaaattgc gtttcatagc
     4741 caatcttctg gctgtcaggc agccaccaga agttatacag tgacagcgct ttttcagcat
     4801 ccaggttggc aatatccact gccgatagca gcatccggtt gccgctgcca tcgcggttga
     4861 tcagccacag ttctttgtta aattccgtca tccatttact gtaagccacc ttttgcccgt
     4921 ctggtgagag ccgtgcttcc acggctcccc catcgctggt gagctgcact gccccactgc
     4981 tggcgcgcca cagccacaga ttgccggttt gcacaaaaac cacctgccgc tcggctggcg
     5041 ttggcagggt tggcacctca cttggcgccg ccgtgatagt cggcactggc tcggtggggg
     5101 ttgctgccgg ggtgggggtg gtatctgcaa aaaagctgcc cacggttgcc gccacattcg
     5161 tttcaacaat atccccgggc ggtggggttg gaatggctgg cagcccgcag gccagcattg
     5221 ccagcatcaa ggccatcaag ggtaggatta gacgaatttt ctttgacata taagcgctcc
     5281 ttttgatatc ttgaaattag attatatcca atcgttgatg aaattggtaa aaatatctgt
     5341 cctgcattcc aaaccacaaa atcctgaaac ccaaaaccta aatcccaaat cccaaattgt
     5401 ggtatcctca taaccaattc ttcatccgga ggttcccttg aaaatctacg atgtctcact
     5461 caccattacc ccatcgctcc ctgtttggcc cggcgatccc caggttgacc tgcaccgtgt
     5521 cagcaaaatg gaggatggcg cggtttgcaa tatttcgcac ctcaccctcg gcgttcacac
     5581 cggcacccat gtcgatgccc cctatcactt cctccccaat ggcggccgcg tggatgccct
     5641 gcccctcgaa gcgctgattg gcccggcgtt ggtccttgaa gtgcctgaaa cctgcactgc
     5701 catcggcgcg gctgaattgc gcgcggctgg cttgcccggt cgggtagagc gggtcctttt
     5761 caagtctcgc aattcaaacc tctggtcttc tgaaaaggaa ttttaccggg aatttgttgc
     5821 cattctgccc gatgccgccg aaatgctggt tgctgccggt gttcgcctgg taggcatgga
     5881 ttacctctcc gtagcgccat tcaataatgc cgtccgcacc catgaaatct tactgagcgc
     5941 cggagtggtg ctgcttgaag gtctgaacct cgctgaagtc cccgccggcc tttatacgct
     6001 ttactgccta cccctcaagc tgggtggcgc tgatggcgcc cctgcccgca ctgtcctcat
     6061 tggggcgtaa tcaacgtata accgccgtcc agcacaatca cctgcccgca gatcatcgcg
     6121 gccgcgggtg agcatagaaa agcaaccacg cctgccacat cttcgggtgt caccagccgc
     6181 ccggcaggcg tgtttttcat cgcccgctca atcaccgctg gatcgcccag tgtggcaaaa
     6241 tgctgcagcg cgtccgtcaa aaccagcccc ggcgccaccg cgttgacgcg aatgttcgca
     6301 ggcgccagtt caaccgccag gtaacgggtc agcgcttcca gcgcggcctt gctggcgcct
     6361 accgccacat aatccggcag cacccggctg gaacccgggc ttgaaatggc caccatctgc
     6421 ccgccgccgc ggctttgcac caatggcgcc gctcgctgag ccgcaaacaa aaacgcccgc
     6481 gcgttcacgt tcagcgtata atcccagcct tgcggctttt gttgcatggc cgggcggtta
     6541 aacccgctgg cagcattgct gataaaaata tccagcccgc cgaactcact gacaatcgta
     6601 tcaaacagcc gctcaatatc ttccagtttg ctcaggttgg cctgaacaac caccgcccgc
     6661 cggcccaatc cttcaatctc agccgccacc tgccgcgccg gaccctcatt gcgtacaaac
     6721 gtcaccacca catccgcccc atcttgagcc agccgcaggg caatggcccg cccaatcccg
     6781 cggcttgatc ccgtcaccag cccgattttg ttttctaaag gcagcatcat ttcattcctt
     6841 tgtttgttga aagctcaccg ggcgcaggtc aatgcgaacg gttgctcctg tggcttcgtt
     6901 ctcttccagc acctgccgta cccggtcggc atcctggcgt aatctgcgaa catctacccc
     6961 ctgacacacg tccggaaact gcgccagcca gccctcggcc cgttccaaca gccggattgc
     7021 cccccgaata tttccgcgcc gcaggtgatg ataggccacc cccacctgta aaatccctcg
     7081 atacagttcc cggatggggc gtttttcttc ccgccaggcc agctccagcc attcatgcgc
     7141 ctcaaaatat tctccctggt taaagtgctc catcccgcgc tgcgccagcc gcggcaaagc
     7201 ttcaccgcag ccttcaatca gatttgcttc taaatcttga ccgaccatca cacctcagca
     7261 attacattca tcttgtctcg ttgacattgt acgataatcc gccgtttttg tgataggatt
     7321 ctatcagcca ttggcgggcg ggcctgtgcc tgcctatctc acaagaatct tacccgagga
     7381 aacgatgatt gacctgcctg atgatcaaat tctccccctt tcgctcgaac ataatttttg
     7441 gacctggtca gcccagggca aagtcagccc catccccgtc aagcgctccc agggcgtcta
     7501 tttttgggat gtaaacgaca agcgctacct cgatttcaac tcgatggtca tgtgtgtcaa
     7561 catcgggcat ggcgatcaac gggtcatcca ggccattatc aatcaggcgc aagaactgcc
     7621 ttttgctggc cccggcatgg ccacccgccc gcgggcgcgc ctggggcagc ttctcgctga
     7681 aattaccccg cctggcctga cgcgcttcct ctataccctc ggcggtgccg atgccaatga
     7741 gaacgccatc aagctggcgc gcgctttcac cgggcgctcc aagatcctca cccgttaccg
     7801 ctcttatcac ggcgccacct acggcgccat cgccgccact ggcgacccgc gccgcgccgc
     7861 ctgggagcct gccgtgatgc ccggcgtggt tcacttcctc gatccttacc gctatcgctc
     7921 caccttccac cgcaccaatc ccaacatttc tgacgaagac ttcacccgcg attatctcaa
     7981 ccacctcgaa gaaatcatcc tctacgaacg ccccgagaca attgccgcag tgatggttga
     8041 atccgtgacc ggtaccaacg gcatcatcat cccgccgccc ggttacatgc agggcttgcg
     8101 cgagttgtgc aataaatacg atatcctcct catttgcgat gaggtgatga gcggttttgg
     8161 ccgcaccggc gagtggttcg ccgtcaatca ctggaatgtt aatcccgata tcatgaccat
     8221 ggccaagggg cttacctctg gctatgcccc gctgggcgcg gttgccatgc gtcaggagat
     8281 cgccgatacc ttcaaagaca aagtctatga aggcggcctg acctacaatg gccatccgct
     8341 ttcccttgcc gctgccattg cggttattga ggccatgcag gccgatcaca tggttgaaca
     8401 cgctcgccag atgggcgagg tcctgtcgga tatgctccag gaactcgtgg accgccatcc
     8461 ctccgttggc gaagtgcgct ccattggcct gtttggcatc ctggaaattg tccgcaaccg
     8521 ccagacccgt gagcccatgg cgcctttcgg cggctccagc cctgaaatga acgccttcaa
     8581 aaaatacatg agcgaccatg gcgtcttcct ctatacccac tggcatacgg tcctgatcat
     8641 cccgcccctc atcatcaccc ccgaggaatt agccgaaggc ttcgccatcc tcgaccaggc
     8701 gctcgaaatt accgatgccg cggtcaaatc ctaagccgga ggcctcatgg aattcaacct
     8761 gactccttta gcccaggcaa gctcatcaac cgaaccccaa tccgaaaaag tcctgatcct
     8821 cggcgcaggt ccggctggcc ttgccgccgc gctctatgct gcccgcgctg aattatcccc
     8881 gctggtgctt accggcctgt ccattggcgg ccaggctgct gccacacatc taatcgagaa
     8941 ttaccccggc ttccccgagg gcattggcgg cgctgaactg ggcgaacagt ttcgtcttca
     9001 ggccgaacgc tttggcgccc gctttgattt tgataccgcc acccgcgttg atctttcaca
     9061 gcgtcctttc cgcgtctaca cctacgaccg tgaatttcac gccgaaaccc tgatccttgc
     9121 cactggcgct tcccccaatc acctcaacat ccccggcgaa aaggaactga cgggcaaggg
     9181 tgtctcctac tgcggcacct gtgatggttg gttcttcaaa ggcaagcggg ttgccgttgt
     9241 cggcggtggt gattctgcca ttgaggaagc catttttctc acccgctatg ctgagacggt
     9301 gacgatcatc caccgccgtg atgccttgcg tgctggtgcc attctccaaa accgcgcccg
     9361 gcaaaatcct aaaatcaact tcatctggga tgcagtggtt accgcggctg aggggcaagg
     9421 cgcacttgcc aacctcaagc tgcgtaacgt caaaaccaac gccgaatcta atttcaccac
     9481 agacggtctc ttcatcttta taggtaatac ccccaacaca gatatgttcc ggggccagct
     9541 cgatatggac gataagggct atgtggtgac caacgcccgc atggaaacca gcctccccgg
     9601 cgtttacgcc gctggtgaag taaccgattc cgtcttccgc caggtgatta cctcagcggg
     9661 catgggcgct gccgctgcca ttcaggccat ccgcttcctc gaagaacacg acgcctgatt
     9721 tccacgcata caaaaagccg aggttttgac ctcggctttt tttgttttac ggattggggg
     9781 ttgtcacacc cgaactccac caggtgaata cccgtggcgt actgatcgcc ccagccgggc
     9841 tgtaaatcgg ctgatcgtca ttcgtcgtgc ccgtctgccg cacaatcgtc acataccagc
     9901 gcatgatatg cggcaaactg tctgccggac ggaagttagc cgggacaatg aacttggtat
     9961 ctgtcacgta ttccaccagc cgccggtctc cgctggtcac atcttcaatt gtcaccgcat
    10021 aggcttcgtt ttgcagcagc gtgctcactg cagcccattg cagcgtgatg acatcgttcg
    10081 cgttcataaa agccgagcca tcggcgggca gcagcaggct gacagccgga taaggcggcg
    10141 gcggggttgg ggtgggggtt gggcccgggg ttggcagccg ttcgcacagc ggaatatcca
    10201 gcttttgccc ttcccacact gagtcgctgg gcaggccgtt atagtcttta atagactgga
    10261 tcgagacatt gtacatgtca gcaatcccgc tcagcgtgtc gttggcggtc accgtgtaag
    10321 agatcttctg gcaagccgca tctgttgctt caacagcgct gagtgtcgcg gttggcggcg
    10381 gcgtccacgt aggggtcggt tgcgggatcc gcagcgtctg acccggaacc agcgtggtgc
    10441 agttggaatc caggttgttc gacacaatga tgctcgcaat cgaaacgttg aacgtcagcg
    10501 caatcgtgcc gcagttatcg cccgatttga ccacatattc aatcggcggc agcggcgttg
    10561 gggtaggcac ctgggtcgcc gtagaagttg ccgtaggcgt cagtgtgacg gttggggtaa
    10621 ttgtcagggt agccgtggcg gtgggttcaa ccacgcgccc ggtgccccgc agcacagcaa
    10681 aaaccacccc ggcgccaatc cccagcaaga ccagcataat cagcagggca atcggcagcg
    10741 aaagggagat gtttggcaaa cggggagcct ttacctcaac tgagggtttc ttctgggctt
    10801 ccgcagccgg agcaaaggtg tggccgcaga ccaggcagcg ggttgcattt tcacccagcg
    10861 gcgaaccaca ggttggacaa gttttagagg gagtgttttc tggactcata ggcttcttag
    10921 tagagtttac cggggagaat ccccggctcg cgtattatac catatgtgaa aatcggtcaa
    10981 gtcccctacg catctgcccc aattcgtgat atcctgaccc tcatgcaagt gccgcttagt
    11041 ctctacgtgc atattccttt ctgccgccgc cgctgcggct attgtgattt caatacctac
    11101 gctggcatgg aatcgtggat tgccccttac agtgcagcac tcaagcaaga gatcacagag
    11161 gttgcccttg ccactggcgc gcgtttgccg gttggcaccg tcttctgggg tgggggaacg
    11221 ccttccttgc tgccaattga gcagattgcc ctgatacaaa atgcgtatag ccaatattat
    11281 gcactcctgt ctgatgtcga aatcaccctc gaggccaacc ctggcactgt ctccctctcc
    11341 tggctgactg agctgcgccg cctgggcttc aatcgtttga gctttggcat gcagtctgcc
    11401 aatccagacg aactgcacct gctcgatcgg gcccatgccc ccagagatgt gcagtccgca
    11461 gtcgcctggt cccgtcaggc ggggtttgat aacctcaacc tcgacctgat ctatggcctt
    11521 cccaaccagc gtctctcaga ctggcaaaat accctctctg ccgcacttca attggagccc
    11581 gaacacctgt ccctctattc gctcattgta gagcccggca ctccgctagc ggctcaggtt
    11641 gaagctggcc gtttgccgcc cccggatgat gacctggccg cagacatgat ggacctggct
    11701 gccgaagtgc tgtctgccgc tggctttgtg cagtatgaaa tctccaactt tgcccgcggt
    11761 ttagagcatt cctgccgcca caatctgcaa tactggcgca atttgcccta tctgggcttc
    11821 ggcgccggag cgcacggcag cgccgctggc tggcgcacag ccaatctcgc atctatctcg
    11881 gcctatctca atgccctcac cgcctcgtca gaatggcccg cctatccctt cagcccggca
    11941 aatgccgaac gcaaccccat tgaccgctgg accgaggtcc aggaaaccct gatggtcggc
    12001 ctgcgcctga cgcatgaagg tgtatcgctg aaaggatttg cagaccgttt cggcatggat
    12061 ctgatggcgc tgtttggcgc gacaattgat cgtcttcagg ctcagggctt gttggaatgg
    12121 gctgaattag agtttggtaa ggccttgcgc ctcacatcac acgggcgctt gctgggcaac
    12181 caggtctttg ctgcttttat cggtgatcca gagccgccgc tattccacaa tcactgacgt
    12241 tccgtatcct acccggtaca catccccctc aacatacgct ggcgttgccc accgaaagac
    12301 ccacagcgcc tcttcgggct tcaaattgac acacccatgg ctcatctgaa caccataatt
    12361 ctggtgccag taagtcccat ggatcgccac gcccgtttcg ggttcaaaat aacttaccca
    12421 tggcacccct ggcagttcat aggcttccgg gtcagccgtc aggttgccgt cacccatatg
    12481 ccgcacaggc atcttgtttt gaatatggaa gctgccttta ggcgtctcgg ttggtatggc
    12541 ccctggaggc aggttgcggc tcggcagccc cgtggagcat ttggtcagca gcaccacctg
    12601 atcattttca tatgctgtca gttcctgccg ggaaattgaa acgtgaatcc actttttgcc
    12661 gtgcggcaca tccggtgtaa tcggggcata ctccgccgca ttaatcatgc tcaggtgcgg
    12721 cgctggcaca aaatacttca gattgagcaa ttcatcccac accttatacc acggcgcgcc
    12781 atccggtcct tcatcaatgc cggtcaccca gtgcaccgag tcataataca accggtacac
    12841 ttgctgccag ccagccacct ctgtataaaa catcgcctgt gaaaacggca ccgtgacgcg
    12901 tgccagttgc cgctctgcct ttaacttgct cgcaaccggg ttgaagcgcg tctgcacctt
    12961 ctgaagatac ccgctgtgca catatccccc ccacacacgg taccacaccg ggttccagcc
    13021 cggcccatcc ggcgagacga cttcctcata gacgtgcagc agttcatccc ggtagcgttg
    13081 atgcacaatc tgactgtcat ccgaaggctg cttatgcacg ctcaccgaat aaacggcaac
    13141 ccgcgccagc gcgccatcgt ccaccgcctc gccatgctca taataaggcc gaaaggccag
    13201 cgcggccata ctcagcccgg ctattttgag aaaatctcgg cgcgatattt ttgtacgcat
    13261 cgtgtcaaga ttataccaga tgcgtcaatt cgtcaatctg ttgtatactt attttataag
    13321 ccatccggcg aggataaata atgattaacc acaaattact ggcatggaca caaaaaggtt
    13381 tattcgtatt ggtctgggct ttgagcagca gcctgggctg gtgggtgatt atctcaatca
    13441 atgcgctcac aagtctatcg gccggtcttt acgatcctga tgaggtggtg ctctacctgt
    13501 cccaatcgcc agccctccgt gctttcaatg ttttgttgcc tgctctcgtt ggcggcctga
    13561 tctttgctgt catcgtgggc gcattgcagt ggtattcggg cgg
//
LOCUS       DAOPVS010000047        13568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4626_length_13568_cov_35.180567, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000047 DAOPVS010000000
VERSION     DAOPVS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4626_length_13568_cov_35.180567"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKL11_05665"
     CDS             <1..221
                     /locus_tag="PKL11_05665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13125.1"
                     /translation="AQPWAQLGPLGVGKGCRGKGLGGALIDAAIRQVQSEGVDGCLID
                     WTDLLDLYGKFGFTPYRQYRMLSKQFAA"
     gene            234..1370
                     /locus_tag="PKL11_05670"
     CDS             234..1370
                     /locus_tag="PKL11_05670"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNZ13126.1"
                     /translation="MDFKPLERAISHVLEAGMTPALQLAVRQHGQLIYSAANGWLDPD
                     TRQRVTCITTRFDMASVTKLFTTTCFLQLVEQGKTGLDQPVCEVLPEFSGLRELHPYE
                     DPLRDGVWVDVANQQTQKVDASQTTFRQLLSHNSGLPAWRPFKEQPSAEAARQMALET
                     FFSYVPGERVVYSDVGLILLGMAVERLTGQRLDAAIRERVTAPLGLQRTGYLPIGELP
                     PDVDLLDIAPTELCRWRNRRVIGEVHDESTSRLGGIAGHAGIFSTAEEIARFGQLFIN
                     HGAPLLSRAMVQEMVKVQAEYNGVRRGLGFALWSNDPEASSNPFSPATFGHTGFTGTS
                     IWMDPERELVAALLTNEVYNGRESRNILPLRVEIHRAIVAAVDA"
     gene            1389..3446
                     /locus_tag="PKL11_05675"
     CDS             1389..3446
                     /locus_tag="PKL11_05675"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNZ13127.1"
                     /translation="MIVIGLMSGTSADGIEAAVVELSGTPAALDWQVLSHVHVDFDDH
                     LRAEIFACFRPETSSVDRLCRLNFALGRALGLAALNAAVAAGLSIQQVDLIGSHGQTL
                     WHIPAGDEASTLQMGEPAVISEITGRTVVSGFRTRDMAAGGQGAPLVAYVDQILLSHP
                     EKTRAVQNIGGIANVTWLPAQNLSGKVPLAFDTGPGNMLIDDAVRRATGGNQTFDRDG
                     QIAAGGQVCGALLQELLSQPYFEMKPPKTTGRELFGSQFGEWVWQRGEALHLCAADVI
                     ATLTEFTAASIAEAYRRFLPIYPDEIILSGGGAFNPTLVRAIRRLTAPATVLISDDLG
                     LKGDAKEAVAFAVLAYETLQGRADNLPAATGARRAVVLGNITPSSPAGDQKPLRAGRY
                     TEGRNTSTENIDQLSTLEMLRRINAEDEKVPQAVRATLPQIASAVDAIAVRMQAGGRL
                     IYIGAGTSGRLGILDASECPPTFSTSPEQVIGIIAGGKVAITDAVEGAEDNAEAGQYD
                     LARLGVNETDSIVGIAASGYTPYVLGGMAEARRRGALVISLACNSPAPIEELADISIA
                     PIVGPEAITGSTRLKAGTAQKLVLNMISTGTMIRLGKTYGNLMVDVQMTNAKLRARGG
                     RIVAEACRISEDNALELLASCNYEVKTAIVAYLGSLSPEEARKRLAEHHGVVRKALMG
                     LKE"
     gene            3443..4366
                     /locus_tag="PKL11_05680"
     CDS             3443..4366
                     /locus_tag="PKL11_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNZ13128.1"
                     /translation="MRSLIIGVDGGGSKTTAILARVDGTILGRGQAGPSNYHAIGVGE
                     ALEALDRAIACAFDNAGLAAEPIDALALGLAGMGRPADRAIFEAWAKRSHPQARLKLV
                     NDAEILLAAGTPDGRGIAIIAGTGSLIFGQTTDGRTDRAGGWGYRMGDEGSGYAAGLA
                     ALQAVARAWDGRGGQTNLTERLLDHFGVAVAPDLIAEVYRDTIGRPEIAALARYVDLA
                     AEDGDAVAGAILQSSAEELALGAKAVMTQLGLKGDIPAALGGGYVTGSRLLQQFVQES
                     AVQQGVHLRPVTPVMEPALGAVKLASALYNL"
     gene            4455..5138
                     /locus_tag="PKL11_05685"
     CDS             4455..5138
                     /locus_tag="PKL11_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNZ13129.1"
                     /translation="MEGYLLYIIISLPALLLGLWAQAKVQGAYRKYSQVRSSTGFTGA
                     QVARRILDTNGLHDVPVEETGGILSDHYDPAKRVLRLSPAVYRTNSIAAAGVAAHEAG
                     HALQHREQYFMLSLRSAMVPTVQIGSWLGPIIFIVGLFLSTTIAWIGLALFAGTSLFA
                     LITLPVEFDATRRAKEWLANSGLVYQGEMVGVHKVLDAAALTYVAAAAQSISTLLYYG
                     LMLVGARDN"
     gene            complement(5220..6227)
                     /locus_tag="PKL11_05690"
     CDS             complement(5220..6227)
                     /locus_tag="PKL11_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNZ13130.1"
                     /translation="MPVFSREFGIDLGTMNTVMAEGNQILLQEPTMVALVIAEQKMVE
                     WGQAAKDMFGRVPDTIEVVRPMQHGVIAEYEITEKMLQYLIKKLTGPMVVFRPRVMIT
                     VPYGITSVESRAVHEAGLGAGSREVYLIRQPLAAALGLDMPIGTPSGNMIISLGGGTN
                     QVGVLALNDVVAAETARTGGLRFDDAIIQYARKKFGVIIGQPTAESLKIQIGAALPLD
                     TPLSVEVQGQDQVTGLPRPITLTTDDIVDALQEPLTELVQLARRVLEKTPPELISDII
                     DRGVALCGGGALLRNVDRFLTKALGIPAYLVDEPMTATALGAARALSMRDALRRSLIS
                     V"
     gene            complement(6248..6682)
                     /locus_tag="PKL11_05695"
     CDS             complement(6248..6682)
                     /locus_tag="PKL11_05695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNZ13131.1"
                     /translation="MPIRCEIVSQDRIVFQGDVDIVVLPGVEGVMGILPDHAPLLSVL
                     QYGYITVRMGREEEVFTVAGGVVEVQPDQVTVLADAAENVEEIDVQRAEAARRRAEEA
                     LQKESGGDSDRQLTLQAALRRSMLRLDAARRFGRTRTQRRGS"
     gene            complement(6691..8091)
                     /gene="atpD"
                     /locus_tag="PKL11_05700"
     CDS             complement(6691..8091)
                     /gene="atpD"
                     /locus_tag="PKL11_05700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNZ13132.1"
                     /translation="MDQATGRVAQVMGSVVDVTFEVGEMPEIYEALEIVRPDAQPMVL
                     EVQKHLGDSGVRCVSMDSTDGIQRGLPVRRTGAPIRVPVGPQTLGRVFNVLGRPVDNK
                     GPVDASDYYPIHRAAPAFADQSTRVEVFETGMKVIDLIAPFTKGGKTGIFGGAGVGKT
                     VIIMELIRSIATVHQGNSVFAGVGERTREGTQLYREMLESGVMKDTVMVFGQMNEPPG
                     VRLRVALSALSMAEYFRDQGRDVLLFIDNIFRFTMSGSEVSALLGRMPSAVGYQPTLA
                     TEMGELQERITSTKHGSITSMQAVYVPADDYSDPAPVATFTHLDATIALERSIAEKGI
                     YPAVDPLVSTSRILDPRIVGDEHYTIAREVQRMLQRYKDLQDIIAILGVDELSEDDKL
                     IVSRARKIERFFSQPMSVAEQFTGIPGRYVPLRETVRGFGEILQGKYDDLPEQAFHMV
                     GTIEDAVEKARKLQAA"
     gene            complement(8123..9019)
                     /gene="atpG"
                     /locus_tag="PKL11_05705"
     CDS             complement(8123..9019)
                     /gene="atpG"
                     /locus_tag="PKL11_05705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNZ13133.1"
                     /translation="MPSAREMRLRIRSVKNLSQVTRALETVSASKVRKAMQAALATRP
                     YAEKAWKVLLHLARQPIQMQSLHPLLVERNQVNNILVIMVTGDRGLAGSYNVNMLRHT
                     LEEFGHIETPVRYIAVGRKGRDMLARRRKNLLAEFSNLPSPPSFLDVSAIGRLAVNQY
                     LSGEVDQVFIAYTEFVNMIKQDTIIRKILPLEVDTSGHGHGTNSTHANHAVFSYEPDS
                     SGLLDQIVPRFTAIQIYQAILSSQASEHAARMVAMRNATDNALDLVEDLQLDYNKARQ
                     QSITNDMLDIASGAEALSAAKA"
     gene            complement(9036..10700)
                     /gene="atpA"
                     /locus_tag="PKL11_05710"
     CDS             complement(9036..10700)
                     /gene="atpA"
                     /locus_tag="PKL11_05710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNZ13134.1"
                     /translation="MSDQIIRQLTADLQGKIEAYQPQVEVRNVGKVLEAGDGIARASG
                     LAGVRSQELVEFANGVIGIAFNLESERVGITILGDYAGIHEGMEVRATGRIASVPVGE
                     GLIGRVVNALGEPIDGKGSLQYSGFYPIERIAPGVVARQDVDSPVQTGIKAIDSMIPI
                     GRGQRELIIGDRQTGKTALAIDTIINQKGKDLICIYVAIGQKKAAVARTIALLERYGA
                     MAHTIVVVAAADESAALQYIAPYAGCAIGEYFMENGQDALVVYDDLSKHAWAYRQVSL
                     LLRRPPGREAYPGDLFYLHSRLLERASRMAAQYVIVPADFGGKTAKPEDAVDGHVYGG
                     PIAKDDAEKALKALSNPEAHRLVKCQGSGGSLTALPIIETLLGDVSAYVPTNVISITD
                     GQIYLENNLFNAGIRPAINVGISVSRVGGSAQTKAMKQVAGRLRLDMAAYRELAAFAQ
                     FGSDLDKATQSQLNRGLRLQEILKQPQYEPMPLEQQITVLFAGVNGFADAVPAERMKT
                     WEQDLLRYMEMSHPEIGRDIAERRQITPDNETHLREALKAFASTWI"
     gene            complement(10918..11802)
                     /locus_tag="PKL11_05715"
     CDS             complement(10918..11802)
                     /locus_tag="PKL11_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNZ13135.1"
                     /translation="MQNRQHHQLEVQAFLQKHLGHAHWDFEIPDGSGNETYFVKSNGH
                     NYFVKLGVQIARYQTMASLGLTPAVIMAGHLTDGMSIIVQPYIVGRTPSRKDYHTNLE
                     QIAAMINRTHHSVEVKQVLPEASSNSYSDIGLEVLTRIQQRWAYYRAQVPEAAEFVET
                     SLADLHQQVQSFRGTGLVASHNDICNANWLTSTNGQLYLIDLESMTLDDPAVDIGAIL
                     WWYYPPELRQRFLEIVGYANDEAFQFRMRVRMAMHCLSITLPRVQSFDEFDAASFAQE
                     LTDFRAILAGKENPQGYE"
     gene            complement(11865..12440)
                     /gene="ruvA"
                     /locus_tag="PKL11_05720"
     CDS             complement(11865..12440)
                     /gene="ruvA"
                     /locus_tag="PKL11_05720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNZ13136.1"
                     /translation="MIAYLEGHVMGGGEDYLTIRLGGMGLRVFVPLTLRTQTGPGAHL
                     ALHTHLVVREDLLALYGFETPEERDLFVLLLGANGVGPRTALTMLSVLSVDAVRRAVL
                     SEQSEVFARVPGVGKKTAQKILLHLQGKISGEGLADVVGLGDADSEVLEALTSLGYSV
                     VEAQAALQSIPRDAAKDVEERLRLALQYFNS"
     gene            complement(12437..13237)
                     /locus_tag="PKL11_05725"
     CDS             complement(12437..13237)
                     /locus_tag="PKL11_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ13137.1"
                     /translation="MLITSTSNPQIKQIRHLRDRKARQETGLFYMEGLRIVTEALQQG
                     LTLQQLIIAPDLLVSTVGQQAALEQARRGAPVLEVSAEVFRSFSLKDGPQGIAAVAHQ
                     RWSNLNEEKLEPLDCWVALDSVADPGNLGTILRTHDSVGGQGVILLDQSTDPYDPTAL
                     RASMGAAFSQRLIRATFAEFIAWAQHWNLPIVGAAGGSKTDYHTYRYPSPLVLLMGSE
                     RQGLQPHHLSACSAVVAIPMQGRSDSLNLAVATAVILYEIYNQRRASA"
     gene            complement(13240..>13568)
                     /locus_tag="PKL11_05730"
     CDS             complement(13240..>13568)
                     /locus_tag="PKL11_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ13138.1"
                     /translation="LHRPESAAVEKLFFQRNVTTALAVGHARGVILLALAQAGLPVAE
                     YTPLEVKQAVAGYGGADKHQMQEMTRALLGLSDIPRPDDAADALGIAICHLSTGRYHA
                     LLDQGS"
BASE COUNT         3023 a         3501 c         4169 g         2875 t
ORIGIN      
        1 tggcccaacc ctgggcgcag cttgggccgc tgggtgtagg caaaggctgc cggggtaaag
       61 ggctgggcgg cgctttgatt gacgctgcga ttcggcaggt gcaatctgag ggcgtggatg
      121 gctgcttgat cgattggacc gatttgctgg acctatatgg aaagttcggc ttcacgccct
      181 atcgacaata tcgaatgctt tcaaagcagt ttgcagcata aaaggataaa agcatggatt
      241 ttaaaccgct ggaacgggcc atttcacatg tattagaggc cgggatgaca cccgcgctac
      301 aacttgccgt tcggcagcac gggcagctca tctactcagc ggccaacggt tggctggacc
      361 cggatacccg ccagcgggtg acctgtatta caacgcgatt tgacatggcc tcggtaacca
      421 agttgtttac gactacctgc ttcctccagc ttgtggaaca agggaaaacc gggcttgacc
      481 agccggtgtg cgaagtgctg cctgaatttt cagggctgcg ggaactgcac ccttacgaag
      541 atccgctgcg ggatggcgtt tgggtggacg ttgccaacca acaaactcaa aaggtggatg
      601 ccagccagac cactttccgg cagttattaa gccacaactc tgggctaccc gcctggcggc
      661 ccttcaaaga acagccttcg gctgaggcag ctcgccagat ggcgctggag acgttcttct
      721 cctatgtgcc gggtgaacgg gtggtctact ctgacgtggg attaattctg ctggggatgg
      781 cagtggaacg gttgaccggg cagcggttgg acgcggctat ccgagagcga gtgaccgcgc
      841 cgctgggact gcagcgcacg gggtatctgc cgattggcga acttccgcca gatgtggatc
      901 tgctggatat tgcccccacc gagctctgcc gctggcgaaa ccgccgggtt atcggggaag
      961 ttcacgatga aagcaccagc cggttaggtg gaattgccgg acacgcgggg attttctcca
     1021 cagccgaaga gattgcccgg tttgggcagc tatttatcaa tcatggagcg ccgctgcttt
     1081 cccgtgccat ggttcaagaa atggtgaaag tgcaggctga gtataacggg gtgcggcgcg
     1141 ggttgggctt tgcactgtgg agcaacgacc cagaagccag cagcaacccc ttcagcccgg
     1201 caacatttgg gcacaccggg ttcacgggaa cgtctatctg gatggaccca gagcgcgaac
     1261 tggtggcggc cctgctgacc aatgaagtct acaatggacg cgagagccgc aatatcctcc
     1321 ccctgcgggt ggagattcac cgggcaattg tggctgcggt ggatgcataa gaccgaggag
     1381 gggaaggcat gattgtcatc ggattaatgt cgggcacttc ggcggatggc attgaggctg
     1441 cggtggttga gctctcagga acgccagcag cgctcgattg gcaagtatta agccatgtcc
     1501 atgttgattt cgatgaccat ttgcgggctg aaatttttgc ctgcttcagg cctgaaacca
     1561 gcagcgtgga ccggttatgc cggctcaatt ttgcactggg gcgggcactg gggctggcag
     1621 cgctgaatgc tgctgtggca gccgggttgt caatacaaca agtggattta attggcagcc
     1681 atggacaaac cctgtggcat atcccggcgg gggatgaggc ttcaacgctg cagatggggg
     1741 aaccagcggt tatttctgag atcacgggtc ggacggttgt cagtggattc aggacgcgcg
     1801 acatggcggc gggcgggcaa ggcgccccgc tggtggccta tgttgaccag atcttattga
     1861 gccaccccga gaaaactcgg gcggtgcaaa acataggcgg gattgccaac gtgacctggc
     1921 tgccagccca aaatctgagc ggcaaggtac ccctggcttt tgataccgga cccggcaata
     1981 tgctgattga tgacgcagtg cgccgggcga cgggtggtaa tcaaaccttt gatcgagatg
     2041 gtcaaattgc tgccggaggg caggtttgcg gggctttgct gcaggaactg ctcagccagc
     2101 cttattttga aatgaagccc cccaagacca ccgggcggga gctgtttggc agccagtttg
     2161 gcgaatgggt ctggcagcgc ggtgaggcgc tgcacttatg cgccgctgat gtcatcgcta
     2221 cgctgacgga gtttacagca gccagtattg ctgaagccta ccggcgtttt ttgccgattt
     2281 acccagatga gatcatcctc agcggcggtg gagcgttcaa cccgacactg gtcagggcta
     2341 tccggcgatt gaccgccccg gcgacagtct taatcagcga cgacctgggg ttgaaaggcg
     2401 atgccaagga agcagtggca tttgcggtgc tggcgtatga gacgctgcaa ggccgcgcgg
     2461 acaatctgcc ggcggcaaca ggagccaggc gagcagtggt gctgggcaat attacgccct
     2521 ccagcccggc gggggatcag aaacccttgc gagcagggcg ctatacggag ggacggaata
     2581 cctctactga gaatattgac cagctttcca cgctggagat gctgcggcgg atcaacgctg
     2641 aagacgagaa ggtgccccag gccgtgcggg caacactgcc gcagattgcc agcgcagttg
     2701 atgcaattgc ggtgcgaatg caggctggcg gaagattgat ttatatcggg gcggggactt
     2761 ccggacggtt gggtatttta gatgcctcgg aatgcccacc cactttcagc acctcacctg
     2821 agcaggtgat tgggattatt gccgggggca aggttgcaat cacagacgcg gtggaaggcg
     2881 cagaagataa cgccgaagcg ggccaatatg atcttgccag actgggtgtc aatgagacgg
     2941 acagcattgt gggcattgct gccagcggat acacccccta tgtgctgggc ggcatggcag
     3001 aagcccggcg gcgcggtgcg ctggtcatca gcctggcgtg caactcgcca gcgccgattg
     3061 aagaactggc ggatatttcg attgcgccga ttgtcgggcc tgaagccatc acagggtcta
     3121 cccgcttgaa agcgggaaca gcgcaaaagc tggtgctgaa tatgatctca accgggacaa
     3181 tgatccgact ggggaagact tacggcaatc tgatggttga tgttcaaatg accaatgcca
     3241 agctgcgggc ccggggcggg cgaattgtgg ccgaagcctg ccggatttca gaggataatg
     3301 cgctggaatt gttggcaagc tgcaattacg aggtcaaaac ggccattgtg gcctatctgg
     3361 gaagcttatc tccagaagag gcgcgcaaac ggctggcaga acatcacggg gtggtgcgaa
     3421 aagccctgat gggattgaag gaatgaggtc gctgattatc ggggttgacg gcggcggcag
     3481 caagaccacc gccatcctgg cgagggtgga cggcacaatt ttggggcgcg gccaggcggg
     3541 gccatcgaat tatcatgcca ttggggtggg tgaggcgctg gaagcccttg acagggcaat
     3601 tgcctgcgcc ttcgataatg caggattggc ggctgaaccc attgatgcgc tggcactggg
     3661 gctggctgga atgggccggc ctgccgatcg ggcaatattt gaagcctggg ctaaacgcag
     3721 tcacccccaa gcaaggctca agctggttaa cgatgcggaa atactgctgg cagccggaac
     3781 cccggacggc cgggggattg cgattattgc aggcacaggc tcattgattt ttgggcagac
     3841 aaccgatggg cgcacggacc gcgcgggcgg atggggatat cggatggggg atgaaggcag
     3901 cggctacgcg gccgggttag cggcgctgca ggctgtggcc cgcgcctggg atgggcgcgg
     3961 ggggcaaacg aatctaaccg agcggctact ggaccatttt ggggttgcgg ttgcccctga
     4021 tttgattgca gaggtgtatc gggatacgat tgggcgcccg gaaattgcgg cactggcacg
     4081 gtatgtggat ctggcggccg aagatggtga tgcggtggcg ggggccattt tgcaatccag
     4141 cgccgaggag ctggctctgg gcgcaaaagc ggtcatgacg cagcttgggc ttaaagggga
     4201 cattccggct gccctgggag ggggatatgt gaccggcagc cgcttgctgc agcaattcgt
     4261 gcaggaatcg gcagtacagc aaggggtaca tctacggccc gtcacaccgg tgatggagcc
     4321 tgccctgggg gcggtgaagc tggccagcgc attgtacaac ctgtgacgcc cggctctaat
     4381 agaaatcata catatttctg gtatgattga taaaaattga tgctggttct ttactgttac
     4441 aggaaaggtt aaccatggaa ggatacttac tttacatcat catcagcctg ccagcgctgc
     4501 tgctgggctt atgggcacag gccaaagttc agggggcata tcgcaaatac tcacaggtgc
     4561 gcagcagtac cggattcaca ggggcacagg tggcgcggcg aattttggat accaatggac
     4621 tgcacgatgt gccggtagaa gaaacaggcg gcatcttaag cgatcactac gacccggcca
     4681 aacgggtcct gcggctcagt ccggcagtgt atcggaccaa cagtattgcc gccgccgggg
     4741 ttgccgcaca cgaagccggg catgccctgc agcaccggga gcaatatttt atgctctcgc
     4801 tgcggtcggc catggtgcct accgttcaga ttggaagctg gctgggaccg attatcttta
     4861 tcgttgggtt gttcctgtcc acaacaattg cctggatcgg gctggccctg tttgcgggaa
     4921 catcgctgtt tgcattgatc acgctgccgg ttgaatttga cgccacccgg cgggcgaaag
     4981 aatggctggc taattctgga ttagtttacc agggtgaaat ggtgggcgtg cacaaggtgc
     5041 tggacgcggc cgcgctgact tatgtggcgg cggctgccca atccatttca acactgctct
     5101 attatgggct gatgctggtc ggcgcccggg ataattaggg gctcaaaccc gtcaaaaaac
     5161 aaaaaagagt gatgcgatca cctcgcatca ctctttttat tatcacagca ttcagagagc
     5221 taaacagaga tcaaactgcg gcgaagggca tctcgcatgg agagggctcg agcagcgccg
     5281 agggcagtgg cggtcatggg ttcatccaca agatacgccg ggatgcccag ggctttggtg
     5341 agaaatctgt caacattgcg gaggagtgcg ccgccaccgc atagggccac cccacggtca
     5401 atgatgtcag aaatcagttc tgggggtgtt ttttcaagca cgcggcgggc cagttggacc
     5461 aattcagtga ggggttcctg taaggcatcg acaatgtcat cagtagtcaa ggtgatgggg
     5521 cgcggcagac cggtaacttg atcctgccct tgaacttcta ccgagagggg ggtatccagg
     5581 gggagggcag cgccaatttg gattttcagt gattcggcgg ttggctgacc aataatcacg
     5641 ccaaattttt tgcgggcgta ctggatgatg gcatcatcaa atcgcaggcc gcccgtgcgg
     5701 gcggtttcgg cggcaaccac atcattgagg gccaggacgc caacctgatt ggtgccgccg
     5761 cccagcgata tgatcatatt gccggaaggg gtgccaatgg gcatgtccag gccaagggcg
     5821 gctgccagcg gctggcggat gaggtagacc tcacgactgc cggcgcccaa acccgcctcg
     5881 tgcacggcgc gggattcaac actggtgatg ccgtaaggca cagtaatcat cacgcgcggg
     5941 cggaaaacga ccattgggcc ggttaatttt ttgatgaggt attgcagcat tttctcagta
     6001 atctcatatt cggcaatcac gccatgctgc atgggacgga ctacctcaat ggtatcgggg
     6061 acgcggccaa acatatcttt ggcggcctga ccccattcaa ccattttttg ttcggcaatc
     6121 accagggcaa ccatagttgg ttcctgaagg aggatttgat tcccttccgc catgacggtg
     6181 ttcatggtgc ccaggtcaat gccaaattct cgcgaaaaga ccggcataat tattgtatcc
     6241 ttgtaattta gctgccccgg cgctgggtac gggtgcgccc aaagcggcga gccgcatcaa
     6301 ggcgaagcat ggaacggcgc aaggcagcct gcagggtaag ctgacgatca gaatccccgc
     6361 ccgattcttt ctggagagct tcttcagcgc ggcggcgagc tgcctcagcg cgctggacat
     6421 caatttcctc aacattctca gccgcatcgg cgaggacggt tacctgatcg ggttgaactt
     6481 caaccacacc accggcaact gtaaagactt cctcttcgcg gcccatccga actgtaatat
     6541 agccatattg cagcacagaa agcagggggg catggtcagg caggatgccc atcacacctt
     6601 ctaccccggg caagacaaca atatctacat cgccctggaa aacaatccga tcttgagaga
     6661 cgatttcaca acgaatgggc atgactgctc ctaggcggcc tgaagtttgc gggctttctc
     6721 aacggcatct tcaatggtgc cgaccatgtg gaaagcctgc tcggggagat catcatactt
     6781 gccctgcagg atttcgccaa agccgcgcac ggtctcgcgc agcgggacgt agcgaccagg
     6841 gatgccagtg aactgttcag ccacagacat gggttgggag aagaaacgct cgatcttgcg
     6901 ggcacgcgag acgattagtt tgtcgtcttc gctgagttcg tccacgccca gaatggcaat
     6961 gatgtcttgc aggtctttgt agcgctgaag catgcgctga acttcacggg caatggtgta
     7021 gtgctcgtcg ccaacaattc gggggtccaa aatccgggaa gtggagacga ggggatctac
     7081 cgcagggtaa atgccttttt cagcaatgga gcgctcgagg gcaatggtag catccaaatg
     7141 ggtgaaggtc gcaacaggag ccgggtccga atagtcatcg gcagggacgt agaccgcctg
     7201 catggaggtg atggagccgt gcttggtaga ggtgatgcgt tcttgcagtt cgcccatttc
     7261 ggtggcgagg gtgggctgat agcctaccgc ggagggcata cgcccaagca gcgccgaaac
     7321 ctcagaccca gacatggtga agcggaagat gttatcaatg aagagcagta catcccggcc
     7381 ctgatcgcgg aagtattcgg ccatggaaag cgccgagagg gccacgcgca ggcgcacgcc
     7441 cgggggttca ttcatctgac cgaagaccat gacggtgtct ttcataacgc cagattcgag
     7501 catttcgcgg tagagttggg tgccttcgcg ggtgcgttca cctacaccgg caaatacgga
     7561 gttgccctga tggacggtag caatggagcg gatcagctcc atgatgatga cggttttgcc
     7621 cacgccagcg ccgccaaaga tgcccgtctt gccgcctttg gtgaagggag cgatcaggtc
     7681 aatgaccttc atgccggttt caaagacttc gacacgggtc gattggtcgg caaaggccgg
     7741 ggcggcgcga tggatggggt agtagtcgct ggcatctacg gggcctttgt tatccacagg
     7801 gcgcccgagg acattgaaaa cgcgacccag ggtttggggg ccaaccggga cgcgaatggg
     7861 tgcgccggtg cggcggaccg gcaggccccg ctggattcca tcggtagaat ccatagaaac
     7921 gcagcgcaca ccgctgtcgc caaggtgctt ctggacttcc aaaaccattg gctgagcatc
     7981 tgggcggaca atttccagcg cttcataaat ttcgggcatc tcgcccactt caaatgtgac
     8041 atctaccaca ctacccatca cctgcgcgac acgtccggtt gcctgatcca tgaataatcc
     8101 tccagatata agcgtatctg tactatgctt tcgcggcgga aagcgcttcg gcgccagagg
     8161 caatgtccaa catatcgttg gtgatgctct gctggcgggc tttgttatag tccaactgca
     8221 aatcctcgac caggtcgagg gcgttgtcgg ttgcgttgcg catggcgacc atgcgggcgg
     8281 catgttcact ggcctgggaa gacaggatag cctgatagat ctgaatagcc gtaaaacggg
     8341 gcacaatttg atccagcagg cctgaactgt cgggctcgta ggagaagaca gcatgattgg
     8401 catgggtgct gttagtgcca tgcccgtgac ccgaggtatc cacttccagt ggcaggattt
     8461 tgcgaatgat cgtatcttgc ttgatcatat tcacaaattc ggtataggca atgaagacct
     8521 gatcgacttc gccggagagg tattgattaa ccgccaggcg gccaatggca gaaacgtcta
     8581 agaatgaggg cgggctgggc aggttactga attcagccag caggttcttg cggcggcgcg
     8641 ccagcatatc acggcccttg cgccccacag caatatagcg gacaggtgtt tcaatgtgac
     8701 cgaattcttc cagtgtatga cgcagcatgt tgacgttata cgaacccgcc aggccgcgat
     8761 ctccggtcac catgatgacc aggatattgt tcacctggtt gcgctcaacc agcagcggat
     8821 gcagagactg catctgaatg ggctggcggg ccaggtgcag cagcactttc caggcctttt
     8881 cggcgtaggg gcgggttgcc agagcagcct gcatggcttt gcgcaccttg ctggcgctga
     8941 ccgtctcgag ggcgcgagtg acctgcgaca ggtttttgac gctccgaatg cggagccgca
     9001 tctcacgagc agaaggcatc cttcaatact ccaggttaaa tccaggtgga tgcgaaagct
     9061 ttgagggcct cgcggaggtg agtttcgttg tccggggtaa tctgacgccg ctcggcaatg
     9121 tcgcgaccaa tttcagggtg agacatttcc atgtagcgga gcaggtcttg ctcccaggtt
     9181 ttcatacgtt cagcggggac agcatcggca aagccattga caccggcaaa gaggacggtg
     9241 atttgctgtt ccaacggcat cggctcatac tggggctgtt tgagaatttc ttgcaggcgg
     9301 agaccgcggt tcagttggga ttgagtggcc ttatcgagat cagagccaaa ctgggcaaag
     9361 gcagccaact cgcggtaagc agccatatcg aggcgcaggc ggccggcaac ctgcttcatg
     9421 gctttggttt gggccgaacc acccacgcgg gaaaccgaga tgcccacgtt aatggccggg
     9481 cgaataccgg cattgaaaag gttgttttca agataaatct gaccgtcggt aattgagatg
     9541 acgttggtgg gaacgtaggc cgaaacatca cccagcaagg tttcaataat tggcaaagcg
     9601 gtcaacgagc ccccgctgcc ctggcatttg accagacgat gggcttcggg gttggacagg
     9661 gctttgaggg ccttttcggc gtcatctttg gcgattgggc cgccatagac atggccatcc
     9721 acggcatctt cgggtttggc agttttgccg ccaaaatctg cggggacgat cacatactgg
     9781 gctgccatgc gggaggcgcg ctccaggagg cgggagtgca ggtagaacag gtcgccgggg
     9841 taggcttcac gacccggggg acgccgcagg aggagggaaa cctggcggta agcccaggca
     9901 tgtttagaga ggtcatcata cacaaccagg gcatcctgac cgttttccat gaaatattcg
     9961 ccaatggcac agccagcata gggggcaatg tattgaaggg cagcggattc atcggcagca
    10021 gcgacaacca caatggtgtg ggccattgcg ccgtagcgtt ccagcagggc aatggtgcga
    10081 gctacggcag cttttttctg gccaatggcg acataaatac agatgagatc tttgcccttc
    10141 tgattgataa tagtgtcaat tgccagggct gtttttccgg tctggcggtc gccaatgatc
    10201 aactcacgct ggccgcggcc aatcgggatc atgctgtcaa tagctttgat gccggtctgg
    10261 acgggcgagt cgacatcctg acgggcgact acaccagggg caatgcgttc aatgggatag
    10321 aaaccagaat attgtagaga gcctttgccg tcaatgggtt cgccaagggc gttgacgacc
    10381 cgaccaatca ggccttcgcc cacgggcaca gaggcaatgc ggcccgtggc gcggacttcc
    10441 atgccttcat ggatgccggc ataatcgccg aggatggtga tgcccacccg ctcagattca
    10501 aggttgaaag cgataccaat cacaccgttg gcaaattcta ccaattcctg agagcgcacc
    10561 ccggccaggc cggaagcgcg ggcgatgcca tcgccggcct ccagaacttt acccacattg
    10621 cggacttcaa cctgaggttg atacgcctcg atcttgcctt gcaggtcggc ggtcagttgt
    10681 cgaataatct ggtcactcat tgaacctcca gcccaggaga aaggatgatc atgctataac
    10741 aggataatca tacctgaaag caaaaacatt gtccagtaaa atgccaaatg gtattctgag
    10801 gaattcagct tccgaggttt atgtagtgat ttgaccgggg taaaatcgtt gcaaaaccgg
    10861 gtaacaggca gtggggtgca gccgtttatt agcccacact tgttattaag tgacgcgtta
    10921 ttcatatcct tgagggtttt ctttacctgc aagtatagct ctgaaatcgg ttagctcctg
    10981 agcaaaagag gcagcgtcga actcatcaaa gctctgaaca cgcggcaggg taatactcaa
    11041 acagtgcatc gccatccgta cgcgcatacg aaattggaag gcctcgtcat tggcgtaacc
    11101 aacaatttcc aaaaaccttt gccgtaattc tggtgggtaa taccaccaaa gtattgctcc
    11161 tatatcaaca gcggggtcgt ccagggtcat agactctaaa tcgatcaagt atagttgtcc
    11221 attcgttgag gtaagccagt tggcgttaca aatatcattg tgcgatgcta ccaaacctgt
    11281 gcctcgaaag ctttgaactt gttggtgcaa atcagccaga ctggtctcaa cgaattcagc
    11341 ggcctctgga acttgcgccc gataatatgc ccacctttgt tgtatgcggg ttagtacttc
    11401 taatccaata tcgctgtagc tgttggagga ggcttcaggg agaacttgct ttacttcaac
    11461 actatgatgt gttcggttga tcattgcagc aatttgctca aggttggtat gataatcttt
    11521 tcgagaaggc gtcctaccca cgatataagg ttgaacaatt atagacatac catccgtcag
    11581 atggccggcc attattacgg cgggagtcaa gccaagtgag gccatcgtct gataccttgc
    11641 tatttgcaca cctaatttta cgaagtaatt atgcccatta ctttttacaa agtaggtttc
    11701 gtttccgctt ccatctggaa tctcaaaatc ccaatgagcg tgccctaaat gtttttgaag
    11761 gaaagcctga acttctagct gatgatgttg tctattctgc atggctattt tcgaccgtat
    11821 taggctttag ggtgtgctac agccaatact ttatgacaag cgggttaact attgaaatat
    11881 tgaagcgcca gacgcaggcg ttcttcaacg tcttttgccg cgtctcgcgg gatggactga
    11941 agggcggctt gcgcctcaac cacgctgtac cccaggctgg tgagcgcctc aagaacttcg
    12001 gaatctgcgt cgcccaatcc gacaacatca gccaggcctt cgccggagat ttttccctgc
    12061 aggtgaagca ggattttttg agctgtcttt ttgcctacgc caggaacacg ggcaaagact
    12121 tcggattgtt cactgagcac tgcccggcgg acagcatcta ccgagaggac ggagagcatt
    12181 gtgagggcgg tgcgcggacc taccccgttg gcgccgagaa gcaggacgaa caggtcgcgc
    12241 tcttcgggtg tttcaaagcc atagagggcc agtaaatctt cacgaaccac caggtgggtg
    12301 tggagcgcta aatgcgcgcc aggaccggtt tgggttcgca aggtaagcgg cacaaagacc
    12361 cgcagtccca tgccgcccag tcgaatggtg agataatctt ccccgccccc catgacatgg
    12421 ccttccaggt aagcaatcat gcggatgccc tcctctggtt gtagatttca tacaaaataa
    12481 cggcggtggc aaccgccaga ttaagtgaat cgctgcggcc ctgcataggg atagccacta
    12541 ccgccgagca agccgacaga tgatgcggct gcaaaccctg gcgttcgctg cccatgagca
    12601 agaccagggg cgaggggtag cgataggtat gataatcggt ctttgagccg ccagcggcgc
    12661 caacaattgg caggttccag tgctgcgccc aggcaataaa ctcggcaaag gtggcgcgga
    12721 tcagccgctg gctgaaggct gcccccattg acgcgcgcaa ggcggttgga tcgtaagggt
    12781 cggtagattg atctaataaa atgacgcctt gaccgccgac tgaatcgtgg gtgcgcagga
    12841 tggtccccag gttgccgggg tcggcaacgg aatcaagagc gacccagcaa tctaagggct
    12901 cgagtttttc ttcatttaaa ttactccagc gttgatgagc aacggcagca atgccctgcg
    12961 ggccatcttt gagcgagaag gatcggaaaa cctctgcgct gacttccagc accggggcac
    13021 cgcggcgagc ctgctcaagc gcggcttgct gccccacggt gctgaccagg agatcggggg
    13081 caatgattaa ttgctggagg gtgaggcctt gctggagcgc ctcagtgacg atgcgcaaac
    13141 cctccatata gaacagaccg gtttcctggc gcgctttgcg gtcgcgcagg tggcgaattt
    13201 gtttaatttg cgggttggat gtgctggtaa tcaacatggt caggatcctt gatccagcaa
    13261 ggcgtgatag cgcccggttg agaggtggca gatagcgatg cccagcgcgt cggcggcatc
    13321 atcaggacgt gggatgtctg aaaggccgag caaggcgcgg gtcatttcct gcatctggtg
    13381 cttgtcagct ccgccgtaac cagcaacggc ctgtttgacc tccagagggg tgtattcagc
    13441 caccggcaag cccgcctgag ccagggccag cagaatcaca ccacgggcat ggcccactgc
    13501 cagggcagtg gtcacattcc gttgaaaaaa aagcttctcc acagcggcac tttcgggccg
    13561 gtggagaa
//
LOCUS       DAOPVS010000048        13522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4653_length_13522_cov_55.171308, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000048 DAOPVS010000000
VERSION     DAOPVS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13522
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4653_length_13522_cov_55.171308"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1214
                     /locus_tag="PKL11_05735"
     CDS             246..1214
                     /locus_tag="PKL11_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNZ13139.1"
                     /translation="MKRSLLYFSILLVLAITLTACTPAARTITDSYEEFAAAPNEAPQ
                     PAFDMPGSGGVAAPEAQFSNVSQGYQPAAERMVIQNANLSIVVKDPAESMDAIIAMTE
                     GMGGFVVSSYLYKTTTYDGIEIPAANLTVRVPVAQLTTAMSQIKAMVENPEKDIITET
                     ISGEDITQAYTDLESRLTNLENAADKLNEIMASATKTEDVLTVYRELTSVTEEIEIIK
                     GQMQYYEEAAAMSAISITLQAQEAVKPITVAGWEPQGVLRDAVQSLINFWQGFVDFMI
                     MLFVYIIPVLLTIALPLFLVFLAGRAIVRRNKAKKAARKTVEPPQA"
     gene            complement(1290..3599)
                     /locus_tag="PKL11_05740"
     CDS             complement(1290..3599)
                     /locus_tag="PKL11_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNZ13140.1"
                     /translation="MTSPECFFSLDPRSYTLHVNSPDDSLPFVKGAPFFAFYWQNGRL
                     YRLPEQPPKSSVGEWQPVPSPLGSLLAVSHHFAPDRQGVVLTITLATCADRPLFFWKL
                     AIENQGKLPITVDHFALMQVALSDMGLDVADDLAFHANGWQSWSHTATYGAAQKQRQS
                     VLGPLQCPMVYNDSTPRLRGRGHFSSDFFAVLGSRRKRTALLTGFLSQQQQFGSVVAN
                     LKSTGRLHLIASGDHARLDPGCTMETDWAVLFPFHVDQPDALAPYFQAVADWHQVRFP
                     ANPPTGWCSWYHFYTKVTAEDIRQNLQAIVSLRDELPLPLVQIDDGFEAAVGDWFDFR
                     PTFPAGVAVLADEIKAAGQTPGLWLAPFILEPNSRLAREHPGWLLRDTRGRRVNAGFV
                     WNNLAAALDITVPDALDYACQAVSTAAHDWGFPYLKLDFLYAAALSGRYSDPTRTRAQ
                     VLRNGLEALRQAAGPDTFLLGCGMPLGSGLGLFEANRIGADVSGSWKPNWNGIYPVFE
                     NEPNVPSARVALQNIITRAPMHRHWWLNDPDCLLVRPDTKLTLPEVQTLASAIALTGG
                     SLLVSDDLPRLPADRRRIAEVLLPAIGERAWVLDWLDAETPARLRVDLGGPGGAWHLL
                     GWFNWGDSPSLFNWQPEAYQLPSGAYWLRSFWDGGVIQAPAGEPPIFPAVPPHGALVL
                     AVRSVTGAPQYLGSDLHLSMGLELSEWHATPQSVSLGLELPRHCAGVVDLALPQPPIS
                     PAAQCIAPGVYRFPVKTAPTARLDVRWAG"
     gene            complement(3633..4187)
                     /gene="dcd"
                     /locus_tag="PKL11_05745"
     CDS             complement(3633..4187)
                     /gene="dcd"
                     /locus_tag="PKL11_05745"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNZ13141.1"
                     /translation="MGLKPDHWIRKMALEHKMVEPYVDTQVREGVISYGLSSYGYDIR
                     VADEFKIFTNVFSAVVDPKNFDPRSMIDFKGDVCVIPPNSFALARTIEYFRIPRGVLT
                     ICLGKSTYARCGIIVNVTPFEPEWEGFVTLEISNTTPLPARIYANEGIAQVLFFEGDE
                     VCQTSYADKKGKYQRQSSIALPKI"
     gene            complement(4212..5861)
                     /locus_tag="PKL11_05750"
     CDS             complement(4212..5861)
                     /locus_tag="PKL11_05750"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNZ13142.1"
                     /translation="MRRLLWVVLAVLLTACTAFPSPTPTETLPPLPTVTATATAIPSP
                     TPTPLPPAFWLDPLLPETFQTKFALPAGWIAASAQGESVASLTFGADRPVATWIYALV
                     AAFPTLTDDVPGADLRAFWQSRAAFPASALLLDASTQSALTSLWGAPQCPVKILPAAD
                     LLDAAWADLTTWAVIPFEQIEPRWKVLSLDGQSPLHKDFDPTAYPLSISISLLASDPA
                     LGETLQAQFSAALPATNRDPDHLTTVILTGVTALVRATASEMEYYGVTHPAEFIGPTL
                     AEADITHISNEIPFDKKCPHPKDNTSPDLVFCSRDKYLELLQVVGTDVVELTGDHFQD
                     WGAPAMLHTLDLYKEQGWPYYGGGANIEEARQPVLFEHNGNKIAFIGCNAKPPGYAQA
                     SEQLPGAYHCNMDYMTAEVQRLRAAGYLPIVTFQHEEFYEWIARPSLVEDFHLMADAG
                     AVIVSGSQAHQPHGMEFYDGAMLRYGLGNLFFDQYTWCDACDEAFIDRHVFYEGRYLG
                     AELLSIQFVDFSRPRWMTPEERQQLLTKTFQASGWLEAEDK"
     gene            complement(5865..7412)
                     /gene="ftcD"
                     /locus_tag="PKL11_05755"
     CDS             complement(5865..7412)
                     /gene="ftcD"
                     /locus_tag="PKL11_05755"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNZ13143.1"
                     /translation="MANPLVECIPNFSDARRPEVVEAILAAIQAVPGVHILDRHSDLD
                     HNRTVVTFVGSPAAVEEAAYQGIARASELIDLDHHTGEHPRMGAADVVPFVPIRDITM
                     VECVEMARRLGKRVGDTLNIPVYLYEEAATRPERTNLENIRRGQYEGLKAEMGLNPAR
                     DPDFGPRAVGPAGGTVIGARAPLIAFNVYLTTDDVSIAQKIGRAVRNSSGGMRFVKGM
                     GVLVEGRAQVSMNLTNYRQSPIARVVELVRREAQRYGVAIHHTELVGLTPEEALIDAA
                     QWYLQLDGFEPDQILERRLFDSDTTAASEAAPAGPDFLDQLASATPAPGGGSAAAHTG
                     AAGAALVAMVARLTIGKKKYESVKDQMWALIEQAETLRAELTAAVDEDAAAFEAVMAA
                     FKLPKDTEEQQAARNAAVQQATFAAAQVPLTVARRAVQVMDLALQAAQLGNLNAISDA
                     GSAVALGRAALTGAGMNVRINLLGLDDQTPGAPLLLEMAELEHRANRIESELRLALKE
                     RAGLELK"
     gene            7496..8107
                     /locus_tag="PKL11_05760"
     CDS             7496..8107
                     /locus_tag="PKL11_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNZ13144.1"
                     /translation="MCGRFTLTLDADEIRTALRLGQIPAEWQPRRNIAPTEPVAVVVD
                     AVQRDVLWMRWGLIPSWAKDMSIGSRMFNARAETLAEKPSFRAALRQRRCLILADGFY
                     EWQKPASGKGKSQPFHFRLLGGEPFMFAGLWESWRQPDGEDLLSCTLITCAANETVAP
                     IHERMPVILRGEQPWEWLRTPAQNLLAPLPTDALIVEKVSAPR"
     gene            complement(8168..9559)
                     /locus_tag="PKL11_05765"
     CDS             complement(8168..9559)
                     /locus_tag="PKL11_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13145.1"
                     /translation="MHTSNKKPVLFPAILLVVLFALLLSACAPQVPESFEYSENNDKG
                     DLVLYVFWGDGCSHCEAAKPFLGQLDETYPTLRIRGFEIWYSEPNAALYTQMCDHYGL
                     PEGGRGTPTFFLGERSWVGYSEDIAADMESAVKTCLETGCPDAGAGIMPGASGELSTP
                     DPTLNPNTEIINIPLIGQVDLSKQSIWISTILIAIVDGVNPCSIWVLTMLLALTLHTG
                     SRKKVAIIGVIFLTVTALIYALFIAGLFTVLSVVSFIGWIQVVVALLALFFAIVNIKD
                     YFWYKEGLSFTIADDKKGGIFKNIRKVMDASQSFWAMAGATVVMAAGVSLVEFSCTAG
                     FPVLWTNLLVSQKIAGGLFILLLVVYMLIYQLDELVIFFTAVYSLKASRLEEKHGRIL
                     KLIGGMLMLTLAGVMLIDPSWMNSLSKSLLIFAIAFGLVVLVLLLHRVVLPAFGIWIG
                     TEAAGQSKKPKRR"
     gene            9677..10465
                     /locus_tag="PKL11_05770"
     CDS             9677..10465
                     /locus_tag="PKL11_05770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13146.1"
                     /translation="MSIDPNKRKSFDAVAEIYNEVRTGYPEMLVEDILSLSRIPDKGR
                     ILEIGCGSGNATIAFARRGYTILGIELGANLAALAVQNCQNYPDVKILNLAFEDWDVE
                     DRAFDLVIAADALHWIPPRIAYPKAARAIKDTGSAAFFWWVPVDPQTEWSREIDQLYE
                     ATTPQFVNPDKRFTAEWLIEIVTRNFEASGCYTTITTRKYVWRETLTRDQYVKGLWTF
                     SMHKDICDAEREKMYARIGDVIDRYGGKVEQPQSVVLFHGGVKR"
     gene            complement(10505..10831)
                     /locus_tag="PKL11_05775"
     CDS             complement(10505..10831)
                     /locus_tag="PKL11_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HNZ13147.1"
                     /translation="MRIIKWVALLGLLAMTGIIIYAFAVGDFSAEGARLLAMPWGIVS
                     LVDLYVGFTLFSCWIIYREKRWLPSIIWVILMMVLGFWTGALYTLVALQTSQGDWRRF
                     WHGRRV"
     gene            complement(10859..11641)
                     /locus_tag="PKL11_05780"
     CDS             complement(10859..11641)
                     /locus_tag="PKL11_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNZ13148.1"
                     /translation="MSFFSIFLTLGLVILGLMTLLWLVSLILKNASIVDIFWGTGFVV
                     VAWASFFLTPDGFLPRKQLLLLLVTLWGLRLSTHILLRNWGKPEDFRYQAWREQNGAS
                     WWWRSFFKVFFLQGVLMWVISTPLLAAQLRPLPDRLIWLDFLAVLVWLIGFFFEAVGD
                     YQLTRFRANPANKGQVLQTGVWRYTRHPNYFGDATQWWAYFLIALAAGGWWTIFSPII
                     MTTLLLRVSGVALLEKSLAVEKPGYKEYIEKTSAFIPWFPRK"
     gene            complement(11665..>11951)
                     /locus_tag="PKL11_05785"
     CDS             complement(11665..>11951)
                     /locus_tag="PKL11_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ13149.1"
                     /translation="IKPILRMHDGKPETEKVRTRDRALHRVVEILRSLGALERIAIVH
                     TNAHERVAQLRALAADLLPAEDIWTMDITPVIGAHIGPGAIGFGVVTART"
     assembly_gap    11952..12051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12052..12659)
                     /locus_tag="PKL11_05790"
     CDS             complement(<12052..12659)
                     /locus_tag="PKL11_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ13150.1"
                     /translation="MTVRIVTDSTCDLPADIINKYRISVIPLYINVGSQGYLDGVDIT
                     RQEFYQKLPVFPTQPTTAVPSAQKFHSVYTALADKGATDVLSIHISTSLSAIHNVAQV
                     AAQEVTSTRVTVMDSRQLSLGTGFMVELAARLAQAGHSLEQIVSALNEQIKRTYVFAA
                     LDTLEFLRRSGRMNRFLANFGTLLQIKPILRMHDGKPETEKVR"
     gene            complement(12668..13009)
                     /locus_tag="PKL11_05795"
     CDS             complement(12668..13009)
                     /locus_tag="PKL11_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNZ13151.1"
                     /translation="MDFLLILKVIAALATVATGLLALVKPTAVYGFTGLTAPGVRGIS
                     EIRSIFGGLFIGVGVAPLFLGPVAYQMLGVGYLAIALARLVSILIDKSYDKSNWISLG
                     IEIVLGVILVI"
     gene            complement(13011..13424)
                     /locus_tag="PKL11_05800"
     CDS             complement(13011..13424)
                     /locus_tag="PKL11_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(13359..13361),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNZ13152.1"
                     /translation="MHLSDFKGQANLLLVFNRGFVUPFCQKHMAQLRHDYSEFVSRHA
                     QIIIIGPEDAAAFASYWQQHDLPFIGLPDPKASVLKLYGQEVNLFKLGRMPAQVIVDR
                     AGIARFVHYGHSMSDIPANTEVFALLDEINTPTGA"
BASE COUNT         3214 a         3436 c         4143 g         2629 t
ORIGIN      
        1 gtgtcctgat aaccattgcc tttcctgtga tgtaatctgc cccctccatt ttctctattt
       61 cgccaaccaa ctttgggaca aacactgggg ggttattaaa ttgcaaatat aatcgttatc
      121 cggtacactg attatggaac tatttattgg tgggatgcgt ctaactcaca gcagaataaa
      181 ccaaaatcaa ttctaaattt gccgatcggc atctgctgcg gtcggcctcc tgaggaggag
      241 aacccatgaa acgctcgctt ttatatttct ctattttgct tgtgctggca atcaccctga
      301 cggcctgtac ccccgctgcc cgcaccatta cagactccta tgaagaattt gctgcggcac
      361 ccaacgaagc tccgcaacct gcatttgaca tgccgggttc tggcggggtt gctgcgcctg
      421 aagcgcagtt cagcaatgtg agccaggggt atcagccagc ggctgaacgg atggtcatcc
      481 agaacgccaa tttatccatt gtggtcaaag accccgccga gtcaatggac gcgattattg
      541 cgatgacgga aggaatgggc gggtttgtgg tttcatcgta cctgtataaa accacaacct
      601 atgatgggat cgaaattccg gcggcaaacc tgaccgtgcg ggtgcccgtg gcacagttga
      661 ctaccgcaat gtcacagatc aaggcgatgg tcgaaaaccc ggaaaaggat atcatcaccg
      721 aaaccatcag tggtgaggat atcactcagg cctataccga tcttgaatcc cgactgacca
      781 acctggaaaa tgcggctgac aagctcaatg aaatcatggc ttcggccacc aaaaccgagg
      841 atgtgctgac ggtctatcgg gaattgacga gtgtgaccga ggagattgag atcatcaagg
      901 ggcagatgca gtactacgaa gaagcggctg ccatgtctgc catcagcatc accctgcagg
      961 cccaggaagc ggtgaagccg atcaccgtgg ctggatggga gccccagggc gtgctgcgag
     1021 atgcggtcca gtcgctgatc aacttctggc aggggtttgt agacttcatg attatgctgt
     1081 ttgtctatat catcccggtg ctgctgacga ttgcactgcc tctcttcctg gtcttcctgg
     1141 cggggcgggc gattgtgcgc cgcaataagg ccaagaaggc tgcccggaaa accgttgaac
     1201 cgccgcaggc ataacctgct ctgaaaatca atatcaaacc gcccgtgctt aaggcatggg
     1261 cggtattttt tagaagaatt tgggatttat cagccagccc aacggacatc cagacgggcg
     1321 gtgggggcgg tttttactgg gaagcgataa acgcccgggg caatgcactg ggctgcaggg
     1381 gatattggcg gctgagggag cgccagatca accacacctg cacaatgccg gggtagttcg
     1441 agaccaagcg aaacgctttg cggggtggca tgccattcag acagctcaag gcccatggag
     1501 aggtgcaggt cactgcccag atattgcggg gcgcccgtca ccgagcgaac agccagcacc
     1561 agtgcgccat gcggcggcac agccgggaag atggggggct cgccagcagg agcctgaatg
     1621 acgccgccat cccagaagga gcgcagccaa taagcgcccg agggcagttg ataggcctcg
     1681 ggctgccagt tgaacaggga gggcgaatcg ccccaattga accagcccaa cagatgccag
     1741 gcgccgcccg gaccgcccag gtcgacgcgc aggcgggcag gggtttcggc atccagccag
     1801 tcgagcaccc aggcacgctc gccgatggcc ggaagcagaa cctcggcaat ccggcggcgg
     1861 tctgcgggca ggcggggcag gtcatctgac acgagcagtg aaccgccggt gagggcaatg
     1921 gctgaggcaa gggtctgcac ttcggggagg gtcagtttag tatccggtcg caccagcagg
     1981 cagtcggggt cgttcagcca ccaatggcgg tgcatggggg cgcgggtgat aatgttctgc
     2041 agggccaccc tggcggatgg gacgttgggc tcgttttcaa acaccgggta aatgccgttc
     2101 cagttgggct tccaggagcc gctgacatcg gcgccaatcc gattggcctc aaagaggcct
     2161 aggcctgaac ccagcggcat gccgcagccg agcaggaagg tatcggggcc agcggcctga
     2221 cgcagcgctt cgaggccatt ccgcaaaacc tgggcgcggg tgcgggttgg gtcgctgtaa
     2281 cgcccggaca gtgcggcagc atacaaaaag tccagtttga gataggggaa gccccaatca
     2341 tgcgcagcgg tggaaaccgc ctggcaggca taatccagtg catcggggac ggtaatatcg
     2401 agggccgcgg cgagattgtt ccagacaaag ccggcattga cgcggcggcc gcgggtgtct
     2461 cgcagcagcc aaccggggtg ttctctggcg agccgggagt tcggctcgag gataaatggc
     2521 gccagccaca ggccgggggt ttgcccggca gccttgattt catcggccaa aacggcaacg
     2581 ccagcgggga aggtggggcg gaagtcaaac caatcgccca cggcagcttc aaaaccgtca
     2641 tcaatttgca ccaggggcaa gggcagttca tctcgcagag agacaatggc ctgcaggttc
     2701 tggcggatat cttcagcggt gactttggtg tagaagtggt accaggagca ccagccggtg
     2761 ggtgggttgg ctggaaagcg gacttgatgc caatcggcaa ccgcctggaa gtaaggcgcc
     2821 agggcatcgg gttgatcaac gtggaacggg aacaggaccg cccaatcggt ttccatggtg
     2881 cagcctggat cgagacgagc atggtcaccg ctggcgatga ggtgaagccg accggtagat
     2941 ttcagattgg caaccaccga accaaactgc tgctgttgag aaaggaaccc ggtcagcagg
     3001 gcagtacgtt tgcggcggct gcccagcacg gcaaaaaagt cactggagaa atgtccacgc
     3061 ccgcgcaagc gcggggtgga atcgttatag accatggggc attggagcgg gcccaaaact
     3121 gactgacgct gcttctgcgc ggcgccatag gtggcggtat gggaccagga ctgccagccg
     3181 ttggcgtgaa aggccagatc atcggcgaca tccaggccca tatcactcaa ggcgacctgc
     3241 atgagggcga aatgatccac cgtgatgggc agcttgccct ggttttctat cgccagcttc
     3301 cagaaaaaca gggggcggtc agcacaggtc gccagtgtga tcgtgaggac aacgccctgg
     3361 cgatctggcg caaagtggtg actgacagca agcagcgatc ccagtggtga tggcacgggc
     3421 tgccactcgc caactgatga tttgggtggc tgctctggga gacggtaaag ccgcccattt
     3481 tgccagtaga aagcaaagaa cggagcgccc ttgacgaagg gaagagaatc atcgggagaa
     3541 ttgacatgca gcgtgtagct gcgcgggtcc agagagaaga aacattccgg cgaggtcata
     3601 ccgctccctc cacgtgatta gattagcagg gactagattt tgggcaaggc aattgaagat
     3661 tggcgctggt atttaccttt tttatcagcg taggaggtct ggcaaacttc atcaccttca
     3721 aagaacagca cctgggcaat gccttcgttg gcataaatgc gggcgggcag cggggtggta
     3781 ttggaaattt cgagggtaac aaagccctcc cactccggct caaagggggt cacgttaacg
     3841 atgatgccac agcgggcgta tgtgcttttg cccagacaga tcgtcagcac gccgcgcgga
     3901 atgcggaagt attcaatggt gcgagccagg gcgaatgagt tggggggaat gacgcagaca
     3961 tcgcctttga agtcaatcat cgagcgggga tcaaaattct tgggatccac aaccgctgag
     4021 aagacgttgg taaagatttt gaattcgtcg gctacacgaa tatcatagcc gtaagatgac
     4081 aggccgtagc taatcacgcc ctcgcggact tgcgtgtcca catatggttc gaccatttta
     4141 tgttccagcg ccatcttgcg aatccagtgg tcgggtttta atcccattat tcaaatcctc
     4201 cgtttttttg attatttgtc ttcggcttcc agccaaccgc tggcctggaa ggttttggtc
     4261 agcaattgct gacgttcttc gggggtcatc caccgcgggc gggaaaagtc tacaaactgg
     4321 atggagagca gttcggcgcc caggtagcga ccctcataaa acacgtggcg gtcaatgaag
     4381 gcttcatcgc aagcatcgca ccaggtgtac tggtcaaaaa acaggttgcc caagccatag
     4441 cgcagcatgg cgccatcata aaattccatg ccgtgaggtt ggtgcgcctg gctgccgctg
     4501 acgatcacgg cgccagcatc ggccatgagg tgaaagtctt caaccagaga cgggcgggca
     4561 atccactcgt aaaattcctc atgctggaag gtgacgatag gcaggtagcc ggcagcgcgc
     4621 aggcgttgga cctcggcggt catgtaatcc atgttacagt gataagcgcc ggggagctgc
     4681 tcgctggcct gggcataacc cgggggtttg gcgttgcagc caataaaggc gattttattg
     4741 ccgttatgct caaacaggac gggctgacgg gcttcttcaa tattcgcacc gccaccatag
     4801 taaggccagc cctgctcttt atacaggtca agggtatgga gcatggccgg agcgccccaa
     4861 tcctgaaagt gatcaccggt cagttcaacg acatcggtgc cgacaacttg caggagctcc
     4921 aggtacttat cgcgcgagca aaagaccaga tcggggctgg tgttgtcttt ggggtgcggg
     4981 catttcttat caaaggggat ttcgttgctg atgtgggtga tgtccgcttc agccagcgtg
     5041 gggccgataa attcagccgg gtgggtgacg ccgtagtatt ccatctcaga agcggtagcg
     5101 cgcaccagcg ccgtgacgcc ggtgaggatc accgtggtca agtggtcagg atctcggttg
     5161 gtggcaggca gggcggcgct gaactgcgct tgcagggttt cacccagggc cgggtcgctg
     5221 gccaggagcg aaatcgaaat gctcaacggg taggcggttg ggtcaaagtc tttgtgcaag
     5281 ggcgactgac catccaggct caaaaccttc cagcgcggct caatttgctc aaagggaatc
     5341 acggcccagg ttgtcagatc agcccaggcg gcatccagca gatcggcagc agggagaatt
     5401 ttgaccgggc actgcggggc accccagaga gacgtcaagg ctgactgcgt actggcatcc
     5461 agcagcagag cgctggccgg gaaagccgcc cgtgactgcc agaaagcgcg caaatcagcg
     5521 ccgggaacat catcggtcag cgtggggaag gcggcaacga gagcataaat ccaggtggca
     5581 acggggcggt ctgcgccaaa ggtcagagag gccaccgatt cgccctgagc gctggctgca
     5641 atccagcccg cgggcagggc gaatttggtt tgaaaggtct ctgggagcaa tgggtccagc
     5701 cagaacgccg ggggaagcgg cgtgggtgtg ggtgaaggga tggctgtggc ggttgcagtg
     5761 acagtcggca gaggaggaag agtctcggtc ggcgtgggtg atggaaatgc agtgcaggct
     5821 gtgagcagca cagccaggac gacccagagc aggcggcgca taggttactt cagctccaaa
     5881 ccggcgcgtt ctttcagggc aaggcgcagt tcactctcaa ttcggttggc gcggtgttca
     5941 agctcggcca tttcaagcag cagcggcgca ccgggggtct gatcgtccaa cccaagcaga
     6001 ttgatgcgga cgttcatccc cgcaccggtg agggcggcgc gccccagggc aacggcggaa
     6061 ccggcatcgg aaatggcgtt gaggttgccg agctgggcag cctgaagcgc cagatccatc
     6121 acctggacag cgcggcgagc aaccgtcagc gggacctgag ctgcggcaaa ggttgcctgc
     6181 tggacagccg cgttacgggc agcctgctgt tcttcggtat ctttggggag cttgaaggca
     6241 gccatcacgg cttcaaatgc ggcggcatct tcatccacag cggcagtcag ttcagcgcgc
     6301 agggtttcgg cctgttcaat cagcgcccac atctggtctt tgacggactc gtattttttc
     6361 tttccaatcg tcaggcgagc gaccatggcc acaagcgcag caccggcagc gcccgtgtgg
     6421 gctgccgccg aaccgccacc gggagccggg gtggcagagg ccaactggtc gagaaagtcg
     6481 gggccagcag gagcagcctc agatgcggca gtggtgtcgg aatcaaagag gcggcgttcc
     6541 aaaatttgat cgggttcaaa accgtccaac tgcaggtacc actgggcggc atcaatcagg
     6601 gcttcttcgg gcgtcaggcc gaccagttca gtatggtgaa tggcaacccc atagcgctga
     6661 gcttcacggc gcaccagttc gaccacgcga gcaatgggcg attggcggta attggtcagg
     6721 ttcatcgaga cctgggcgcg cccttccacc aggacgccca tgcctttgac aaagcgcatg
     6781 ccgccagatg agttgcggac ggcgcggccg atcttctggg cgatggagac atcgtcagtg
     6841 gtcagataga cgttgaaggc gataagcggg gcgcgggcgc cgatgacagt gccgcccgcc
     6901 gggccgactg cacgggggcc gaaatcggga tcgcgggcgg ggttgaggcc catttcagct
     6961 ttcaggccct cgtactgacc gcggcggatg ttttcaaggt tggtgcgctc agggcgggta
     7021 gcagcttctt catataagta taccgggata ttgagcgtat ctcccacacg cttgcccagg
     7081 cggcgagcca tttccacaca ttcgaccatg gtgatatcgc gaatcgggac aaaggggaca
     7141 acatcggcag cgcccatgcg gggatgttcg ccggtgtgat ggtcgaggtc aatcagctca
     7201 gatgcgcggg caatgccctg ataggcggct tcttcgaccg cggcgggaga gccgacaaaa
     7261 gtgaccacgg tgcggttatg gtcgagatcg gaatggcgat ccagaatatg aacgccagga
     7321 acagcctgaa tggcggcgag aatggcttcg acgacttcgg gacggcgagc gtctgaaaaa
     7381 ttggggatac attcaacaag cggattggcc atgagtaacc tctgtatatg gagatggtac
     7441 aattataata tagcgaagcg cttttggagg ggggccgttc tgaaaggagc caaagatgtg
     7501 tggtcggttc acgctaacgc tggatgcaga tgaaatccga acagccctgc gcctggggca
     7561 aattccggca gagtggcagc cccggcggaa cattgcgccc acagagccgg tggcggtggt
     7621 ggtggatgcc gttcagcggg atgtgctgtg gatgcgctgg gggctgattc catcctgggc
     7681 aaaagatatg agcattggca gccggatgtt caatgcgcgg gcagagacgc tggcagagaa
     7741 gccgtccttc cgggcggcac tgcggcagcg gcgctgcctg atcctggccg acgggtttta
     7801 tgaatggcaa aaacctgcca gcggcaaagg aaaaagtcag ccatttcact ttcggctgct
     7861 aggcggcgag ccatttatgt ttgccggatt gtgggaaagc tggcgccagc ccgatggcga
     7921 agacctgcta agctgtaccc tgattacctg cgcagcgaat gaaacggtgg cacccatcca
     7981 tgagcggatg ccggtcatcc tgcgcggcga gcagccctgg gaatggctgc gcacgcctgc
     8041 tcaaaacctg ctggcaccgc tgccgacgga tgctctgatc gtagaaaagg tatctgcgcc
     8101 gagatgatgg gacgataaat caaaaaacag cagccctggc tgctgttttt tgtatgagac
     8161 cggaaattta acgccgcttt ggtttcttgc tctgaccggc agcctcggtg ccaatccaga
     8221 tgccgaaggc gggcagaacg acccggtgca ggagcaggac cagcaccacc aggccaaagg
     8281 cgatggcaaa gatgagcagt gatttggaga ggctgttcat ccagcttgga tcaataagca
     8341 tgaccccggc cagggtcagc atcaacatgc cgccgatgag cttgaggatg cggccgtgct
     8401 tctcttccag gcggctggct ttgagcgagt acactgcagt gaagaaaatg accaactcat
     8461 ccagttggta gatgagcatg taaacaacca gaagaaggat aaacaggccg ccagcaattt
     8521 tttgagaaac cagcaagttc gtccagagga cagggaagcc cgccgtgcag gagaactcaa
     8581 ccagcgagac ccccgccgcc atgacgaccg tggcgccggc catggcccag aaagattgag
     8641 aagcatccat caccttgcgg atattcttga agatgccacc ttttttatca tcggcgatgg
     8701 tgaaggagag gccttctttg taccagaaat agtctttgat attgacaatg gcaaagaaaa
     8761 gcgccagcaa ggctaccacc acctggatcc agccgatgaa gctgaccaca ctgagcacag
     8821 tgaagaggcc agcgataaag agggcgtaga tcaaagctgt cacggtcaaa aagatgacgc
     8881 caatgatggc gactttttta cgggagccgg tgtgtaaggt aagggccagc agcatcgtca
     8941 aaacccagat ggagcaaggg ttgacgccat caacaatggc gatcaggata gtactgatcc
     9001 agatagactg cttggagaga tccacctgac caatcagggg gatattgatg atttcggtat
     9061 tggggttgag ggtgggatcg ggggtggata gttcaccgga ggcgccaggc atgatgccag
     9121 caccggcatc tgggcagcca gtttcaaggc aggttttgac ggcggattcc atatccgcgg
     9181 caatatcctc agaatagccc acccagctgc gctctcccag gaaaaaggta ggggtgccgc
     9241 gaccgccttc tggcagtcca tagtggtcac acatctgggt gtacagggct gcattgggct
     9301 ctgaatacca gatttcaaag ccgcggatgc ggagggtagg ataggtttcg tctaactgcc
     9361 ccaaaaaggg tttggcggct tcgcaatggc tgcagccatc accccagaag acatataaaa
     9421 ctaaatcgcc tttgtcgttg ttttcggagt attcaaaaga ttcaggaacc tgcggggcac
     9481 aggcagacag aagcagggca aagagcacca ctaaaagaat cgctgggaaa aggaccggtt
     9541 ttttgtttga ggtatgcatc aaatcagttt ccttatccga atgtgaatgg ctgtataagt
     9601 ttgatattat acttcaagat atgcaaatga atgctgtgga taaatttatt ccaagaaagt
     9661 agacaaggtg agcttgatga gcatagatcc caacaagcga aaatcatttg atgcggttgc
     9721 cgaaatttac aacgaggtgc ggactggcta tcctgagatg ttagttgaag atatcttgag
     9781 tctttcgcgg ataccagata agggcagaat cttagaaatc ggctgtgggt caggtaatgc
     9841 cacaattgca tttgcccgac gcggttatac catcctgggg attgaactgg gggccaacct
     9901 ggctgcgctg gctgtgcaaa actgtcagaa ttatcccgat gtgaagattc tcaacctggc
     9961 ttttgaagac tgggatgttg aggatagggc tttcgacctg gtgattgcgg cagatgcgct
    10021 gcactggata ccgcctcgaa tcgcctatcc aaaagcagcc agagccatca aagacactgg
    10081 ttcggcggca ttcttctggt gggtgccggt tgatccacaa acggagtggt cacgggaaat
    10141 tgatcagttg tatgaggcca ccacgccgca atttgtcaat ccagataagc ggtttacagc
    10201 ggaatggttg attgaaatcg tcacccgcaa ctttgaggcc agcggatgtt atacaaccat
    10261 caccactcgg aaatacgtat ggagagagac cctgacgcgc gaccaatatg ttaaggggtt
    10321 atggacgttt tcgatgcaca aagacatttg tgatgctgag cgcgaaaaga tgtatgccag
    10381 gattggcgat gtgattgacc ggtacggggg aaaagtggag caaccccaat ccgtggtgct
    10441 gtttcatggg ggggtaaagc gatgattttg gggtggcgtt gaattacgcc accccattat
    10501 ttgctcaaac ccggcgccca tgccagaacc gccgccaatc gccctgactg gtttgcagag
    10561 caaccaacgt atataaggcc cccgtccaaa agcccaatac catcatcagg ataacccaga
    10621 tgattgaagg cagccatctt ttctcacggt agataatcca gcaagaaaag agcgtaaagc
    10681 cgacatacag atctaccaga gagacaatgc cccagggcat tgccagcaag cgggcgcctt
    10741 cggcagaaaa gtcacccacg gcgaaggcat agatgatgat gcccgtcatc gccagaagtc
    10801 caagcagggc aacccattta ataatgcgca tggcggtcac ctgtgcgtct aaacaagatt
    10861 atttacgcgg gaaccagggg atgaaggcgc tggtcttttc gatatattct ttgtaaccag
    10921 gtttttccac cgccagagat ttttccagca gggctacccc agacacgcgc aggagtaatg
    10981 tagtcatgat gatggggctg aagatggtcc accaaccacc agccgccaga gcaatcaaaa
    11041 aataggccca ccactgggtg gcatcaccaa agtaattggg gtgacgggtg tagcgccaga
    11101 cgccggtttg cagcacctgc cccttattcg caggattggc gcggaagcgg gtcagttgat
    11161 aatcaccaac agcttcaaag aagaagccaa tcagccagac aagaacagcc agaaaatcaa
    11221 gccagatgag gcggtctgga agcggacgaa gctgtgcagc caggagcggc gtggaaatta
    11281 cccacatcag cacaccctgc aagaagaaaa ccttgaagaa gctgcgccac caccagcttg
    11341 ccccgttttg ttcgcgccag gcctgatagc gaaaatcttc gggcttgccc cagttgcgca
    11401 gcaggatatg ggtagagagc cgcagccccc agagggtgac caacaggagc agcaactgct
    11461 tgcggggcag gaagccatca ggagtaagga agaaggaagc ccaggcaacg accacaaagc
    11521 cggttcccca gaagatgtct acgatgctgg catttttgag aatgaggctg accagccaga
    11581 gcagggtcat gagtcccagg atgaccaggc caagggtaag aaaaatcgaa aagaagctca
    11641 tgtgtttttc ctttacagat gcgttcaggt gcgcgcagta accacgccaa agccaatggc
    11701 gccggggcca atgtgagcgc cgatgactgg cgtgatgtcc attgtccaga tgtcttcagc
    11761 gggcagcaag tcggctgcaa gagcacgcaa ctgagctaca cgctcatggg cattggtatg
    11821 gacgatggca atccgctcaa gcgcgcccag cgagcgcaga atttcaacca cgcggtgcag
    11881 agcacggtca cgggtacgga ccttttcagt ctcgggtttg ccgtcgtgca ttctgagaat
    11941 cggcttgatt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggaccttt
    12061 tcagtctcgg gtttgccgtc gtgcattctg agaatcggct tgatttgcag caaggtgcca
    12121 aagtttgcca ggaagcggtt catgcggccg ctgcggcgga ggaattccag cgtgtcgagg
    12181 gcagcaaaga cgtaggtgcg cttgatctgt tcgttaagcg cagatacaat ttgctccagc
    12241 gagtgtccgg cttgtgccag gcgcgcagcc agttccacca tgaagccggt gcccaggctg
    12301 agctggcggg agtccatcac ggtgacccgg gttgaggtga cttcctgcgc agcgacctgg
    12361 gccacgttat ggatggcgct caaagaggtt gaaatgtgaa tggacaggac atcggttgcg
    12421 cctttatctg ccagggctgt atagaccgaa tgaaactttt gggctgaagg cacggcagtg
    12481 gtaggctggg taggaaagac cggcagtttt tgataaaact cctggcgggt gatatcaacg
    12541 ccgtctaaat agccctggga accaacgtta atgtacaggg ggatcaccga gatgcgatat
    12601 ttattgataa tatcggccgg cagatcgcag gtgctgtctg tgacaatgcg aacagtcata
    12661 taccctccta gatgaccaga atgacaccca gaacaatctc gatgccaaga ctgatccagt
    12721 tggacttgtc ataggatttg tctatgagaa tggaaaccag acgcgcgagg gcaattgcca
    12781 gatagccgac gcccagcatt tggtaagcca ccgggcccaa aaacagaggg gcaacaccta
    12841 cgccaatgaa aaggccgcca aaaatgctgc ggatttcaga aatgccgcgc acacccggcg
    12901 ccgtgaggcc ggtgaagccg tagactgcgg tgggtttgac gagggccagc aagccagtgg
    12961 ctaccgtagc cagggcagcg atgactttga gaatcagtaa gaaatccata ttatgctcca
    13021 gtgggggtat tgatttcgtc caacagcgca aagacttcag tgttggcggg gatatcggac
    13081 atggaatggc cgtaatgcac aaagcgggcg atcccagcgc gatctacgat gacctgggcg
    13141 ggcatgcgcc ccagcttgaa caggttaact tcctgaccat agagcttgag aacgctggcc
    13201 ttggggtcgg ggaggccgat gaagggcagg tcgtgctgct gccagtaact ggcaaaagcg
    13261 gcggcatctt cagggccaat gatgatgatc tgcgcatggc ggctgacaaa ttcagagtag
    13321 tcatggcgca actgcgccat atgcttttgg cagaatggtc agacaaatcc acggttgaaa
    13381 accagaagaa ggttagcctg acctttgaag tcagaaaggt gcaccggctg acctttgaaa
    13441 tcggtcaggg tgaaatcggg tgcaggggca ttgagagaca ccttgggcat tgcaatcctc
    13501 ctctatcgat ctattgtact ta
//
LOCUS       DAOPVS010000049        13482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_4672_length_13482_cov_57.595368, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000049 DAOPVS010000000
VERSION     DAOPVS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4672_length_13482_cov_57.595368"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKL11_05805"
     CDS             complement(<1..793)
                     /locus_tag="PKL11_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ13153.1"
                     /translation="MFERNKRLVGWMLMLVVALAGCVTPAVPTPAPMQTEAAPTPDQP
                     TPTALPTRPPYPPGTLVEYTAQTGDTLIALAARFNTTVTEIREANPIIPDHVTTLPPG
                     LPMQIPIYYQILWGSPYQILPDSQYGYGPAQVGFDAAAFVAAQPGWLKDYSIFIGGAT
                     RRGGAIVEHIATNFSINPKLLLAILEYQTGALSQPEPPVDLEDYPLGYVEYMHTGLAS
                     QLTWAANYLNNGFYAWRNGNLTLFEFPDGRLERPDPWQNAATVALH"
     gene            complement(777..2342)
                     /locus_tag="PKL11_05810"
     CDS             complement(777..2342)
                     /locus_tag="PKL11_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ13154.1"
                     /translation="MKPGIVSYPKIKLPAKRWLTLTVLAAWAISLLACAWGPEAVTPV
                     PTYDLGIGGMDTPNAETPQITPSVQFLAYTPAPTKTATVPITPRPPLLYTTQAGDSLN
                     ALAIRFGVSVDQIECNQALPAEGFIDPGQLLVIPDVLPDRQVMERVMPDSEVVYSATA
                     LDFDLDGYVAGAGGYLSSYREYLSDGWYDGAQVVKRVAQENSINPRLLLAIIEYQSHW
                     VTGQPQSLAETNYPAGNVDFSQKGLYHQLTWAARQMSLGYYGWRLGTVTTLSFPNGET
                     RRLWPELNAGTVGLLYLFSQIYEPLAWAGAVYGTESLSVRYEEMFDSPWQRAQTVEPL
                     LPAGFSQPPMELPFAPGLPWVYTGGPHPAWGTGTPMAALDFAPPSDERSCEASMYWIT
                     AAAPGLVTRSDNGLVVVDLDGDGSEQTGWVILYLHIATMGRVPVGEWLDQDEQIGHPS
                     CEGGSATGRHVHMARKYNGEWMPADSPAPFVLSGWRAVQGEKNYEGLLIKDGVIVTAS
                     TVGSVEAVIVRTK"
     gene            complement(2296..3828)
                     /locus_tag="PKL11_05815"
     CDS             complement(2296..3828)
                     /locus_tag="PKL11_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ13155.1"
                     /translation="MRLKPQRLSAVLMSFVMLGLLLSACGKPDKESTLWWLNATSETI
                     PQGTVTLTPFLPPTRVPGAPILTPTPDAPRELPGQRSETIYYTVQAGDSLAGIGQGYG
                     VSVNTLAEANGINPADWLYAGQVLIIPPPEVQIGASGFKIIPDSELVYGPVSAYFNVG
                     EFVEQQGGYLSRHWEVVDEMETSGAAVLERVAQENSVNPRLLLAVLEYQSGWVTGGDP
                     SEDQKKHPLATMEYWRVGLYHQLSWAANALNRGYYLWRAEALGQIPLADGSAAPLDPT
                     LNAGTAGVQYYLGQLYGAADWQHAVSEDGLFATYQNLFGYPFDLAIEPLIPEGLSQPT
                     MVLPFNVGETWSFTGGPHGGWTDGSGWAALDFAPPGEPQGCVVSPNWVTAVADGVVVR
                     SGHGMVVLDLDGDGLEQTGWTVLYLHVDSWQRAPVGDIVQAGDRIGRPSCEGGYSLAT
                     HLHIARRYNGEWISADGDLPFVMDGWVSAGDGIEYNGRLTRDGVVIEAWDRFVPQNQI
                     TR"
     gene            complement(3856..4197)
                     /locus_tag="PKL11_05820"
     CDS             complement(3856..4197)
                     /locus_tag="PKL11_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNZ13156.1"
                     /translation="MACIFCQIAAGEAPAKIYYQDDRVTAFQDTHPIAPVHILIIPNQ
                     HIDSVNAIGPADEALLGHLITVAQKLAAERGIDRSGFRLMMNTGHDGGQSIYHLHLHL
                     LGGRHLPIHFH"
     gene            complement(4268..5347)
                     /locus_tag="PKL11_05825"
     CDS             complement(4268..5347)
                     /locus_tag="PKL11_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNZ13157.1"
                     /translation="MADKKVRVAIIGVGNCASSLVQGVHFYGNAADTDRVPGLMHVNL
                     GGYHIRDIEFSAAFDVVDTKVGKDLSEAIFSYPNNTYKFCDVPRLGVRVERGMTHDGL
                     GKYLSQILKKAPGPTANIVQILKDTGTDVVVSYLPVGSEMATKWYVEQILEAGVGFVN
                     AIPVFIASSEYWGERFRERGLPIIGDDIKSQVGATILHRVLTSLFVDRGVRMDRTYQL
                     NFGGNTDFMNMLERERLESKKISKTGAVTSMLPYPISEENIHVGPSDYVPWLTDRKWC
                     YLRMEGTTFGEVPLNLEAKLEVWDSPNSAGVVIDAVRCIKLARDRGIAGPLYGPASYF
                     MKTPPRQFKDDQCREMTEAFIRGEA"
     gene            5439..6707
                     /locus_tag="PKL11_05830"
     CDS             5439..6707
                     /locus_tag="PKL11_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ13158.1"
                     /translation="MGVDTIDYTRRLTRLLEVCKNLSSNLELEPLLQSLLEVASELTH
                     SESSSILTYDEENGLLKFLAVPWHLKERSSAIGVPLDASAAGWVFLNRQPMLVQDAQD
                     DRRIFRAVDLEWNNQTRSLLAVPLCFRGQTIGVFECTNKAQQANYTEEDVFILETLAA
                     QAAIAIRNRHLLEQSRQAYEKALESDRLKGDFIAIMSHELRTPLGVVLGHSALLRDMA
                     PENLRVDLDVIAKSAMRLKEIIEDFSNVDRIEAGLASLRRQRVMISTLVQSVVDSFKD
                     LARDHQIALDFHVSQRNLALEGDESKIAIALSNLVRNALTFTNPGGQVQVRAEQVPGY
                     IKIAVIDDGIGIPAAEQEKIFERFYQVEKHLTRRHGGMGLGLSIAREFIELHGGRIWV
                     ESTEGKGSRFTFLLPKSQAEAAAAGRVFGS"
     gene            complement(6704..7144)
                     /locus_tag="PKL11_05835"
     CDS             complement(6704..7144)
                     /locus_tag="PKL11_05835"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNZ13159.1"
                     /translation="MKKITLHGELHANPCLNCGACCAFFRVSFYWGETDPAQGGQVPV
                     ELTENVTEYYCAMRGTNQLSRRCIALEGKPGGPTRCTIYEQRPSPCRDFGVQWQGGQM
                     SIRPGDLERCNHARAAWNMPPISVPVHHVSPWRQTPVHKHLTRR"
     gene            complement(7190..8950)
                     /locus_tag="PKL11_05840"
     CDS             complement(7190..8950)
                     /locus_tag="PKL11_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNZ13160.1"
                     /translation="MNLLGQTIGRYQIEAQLGEGGMATVYRALDTRLDRMVAIKFIRK
                     DAFTTELLEQVLARFEREAKALARLTHPNIVNVHDYGEYEGAPYFVMQYVPGGTLRQR
                     TGKPIPWQEAIRLVLPVARALSYAHAQNIVHRDIKPSNILMTESGEPMLMDFGIAKIL
                     DLSSGATLTGAGTGIGTPEYMAPEQAMGNPVDGRADVYALGVVLYELLTGRTPYQADT
                     PMAVMLKHITEPLPDPRQFVPSLPQAVVTALARALQKQPNDRYADMNGFISVLEGLLM
                     QPGGATEPVQAAAVEVPEAAPTIPVMDPPAVIEPALKTAASLSAPTLVERAQVEAPAA
                     RPKKARKPIPRWAWFVGGGLVVTVLIAAALIVGLSQYFSRGGDFQLPALFARPTATYT
                     ATVTSTPKPTATPRPSLTATATATLKPSATATITRTPTITQTPTITPTPTITQTPSKT
                     PSPTPAKAALGEPTFTTTTVSYYAGCANDQLGIEMDVTDPDGIYLINVYYRIVDQGTG
                     DMTDWALVPILMEKQTGDHYTMTMPALRQMGLNGWIADRFSTYGGSWSGLLEIQFIVV
                     DMNRDVTNSKNYDFVLKGCG"
     gene            complement(9038..10474)
                     /locus_tag="PKL11_05845"
     CDS             complement(9038..10474)
                     /locus_tag="PKL11_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13161.1"
                     /translation="MLPLENTENSPRKWWVLVSIGLGTFMSALDGSVVNTILPVLQTS
                     FDSNIATIEWVVTVYLLVVSSLLLTVGRLGDMNGNKRVYTLGFVIFLLGSALCGLAGS
                     PTGLILARALQAIGAAMLFANSPAILTKVFPAQQRGRALGLQATMTYLGLTVGPSLGG
                     WLTEQFSWRAVFYINIPVGLLALALSLRFIPADRKTDGKERFDLVGAALFGVGLVALL
                     LGLNQGAAWGWRSLPTLGLLGTAVGLLVGFMAWERKQPAPMLDLGLFRSRTFSTAVSS
                     ALLSYMCLYAVIFLLPYYLIHGRDFSPAQAGLILTAQPIVMALIAPISGSLSDKIGSR
                     LLTALGMLTVSGGMVMLSFVGASSPTGQIIAGLAVVGLGMALFASPNNSALMGSAPGH
                     RRGIAAGMLATARNVGMVLGIGLAGAILTSIYRPAEADTLFSAVQAGLLVGAGLALAA
                     AGLAAMREKVIQASAAGAALTPDAQKPE"
     gene            10852..12840
                     /locus_tag="PKL11_05850"
     CDS             10852..12840
                     /locus_tag="PKL11_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ13162.1"
                     /translation="MSLITTENLGLSFGAFDLFLGITVHVPNDGKIGLIGPNGIGKTS
                     LVRILAGLDQPTAGRVYLARGRRIGYLRQEAVDAFADRTNTVYEEMLTAFEDLRAQQT
                     RLNELEARMSAGDHAEALLQQYGDLQHAFEQNGGYNYEVDIQQTLEGLDLGKNYWHMP
                     LAHLSGGQKTRALLARLLLEKPDLLILDEPTNHLDTQAVEWLEHTLGDWRGAVLIVSH
                     DRYFLDNTVDTIWEMSRAGIETYSGNYSSYLVQREERWEYYEKVFEEEKTRLLNEVDY
                     IQRNWVRDSTHAQALGRLRRLSRDIAIVENFGLMALRSGKKWSEMDLHVERPLDVIEA
                     IRAVNAIQMPGNRPTHIRPRLASDKVSGSIILRAQDAAIGYPGNNLFNIREVELRRGE
                     CAALLGPNGSGKTTFLKVLLGQLDPLSGSVNLGASLQIGYFAQSHDALKGDHNILDEL
                     ARHKPLEMEQARSHLAQYLFRGDDVFKQLSSLSGGERARLALAILALDGANFLLLDEP
                     TNHLDMPAREALQDVLDSFPGTILLVSHDRYLVDQLATQIWEIRDGALNVFKGSYREF
                     VLRRATAPGAAARTIVMPPKPIMRGNDKEERRRVQALNLLEERIHEQETRIQRLSSEL
                     QRAGQSQAFERIQKLSREIGQAQTTLEELISEWEKLAA"
     gene            12819..>13482
                     /locus_tag="PKL11_05855"
     CDS             12819..>13482
                     /locus_tag="PKL11_05855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13163.1"
                     /translation="MGKAGSLTMPDHQTIYQQAAGRYDRLVSCEDAAGNLLIELNKIT
                     SFEGQQIVELGAGTGRLTRLLAPLARTIHAFDLSPHMLAFAAQRLKGLSIQNCTLAAA
                     EHRAIPLPAASADVVISGWSLCYLAQGDRRIWQPVVQQVLDETYRLLRPHGWLILIET
                     LGTGFETPTAPEKLLPYYDFLEAQGCAQTWTRTDYRFDTVAEAVDLTRFFFGDELADQ
                     LER"
BASE COUNT         2834 a         4253 c         3583 g         2812 t
ORIGIN      
        1 agtgcagcgc cacagtcgcg gcattttgcc aggggtccgg acgctccaac cgtccatctg
       61 gaaattcaaa cagggtcagg ttgccgtttc gccaggcata gaatccgttg ttcaggtaat
      121 tcgccgccca ggtcaattgc gaagccaggc cggtgtgcat gtattccaca taccccagcg
      181 gataatcctc taaatcaact gggggctctg gctggctcaa tgccccggtc tgatactcca
      241 gaattgccag cagcagcttg ggattgatgc tgaaattggt ggcaatatgc tcaacaattg
      301 cgccgcctct gcgggtggct ccgccaataa aaatggaata atctttcaac cagccgggct
      361 gtgccgccac aaatgccgct gcatcaaacc ccacctgtgc tggcccatac ccatactggc
      421 tgtcaggcaa aatctgatac gggctgcccc acaaaatctg atagtagatc gggatctgca
      481 taggcaggcc cggcggcagg gtggtcacat ggtccgggat gatggggttg gcctcccgga
      541 tttccgtcac ggtggtatta aatcgcgctg ccagtgctat caatgtgtcg ccggtttgag
      601 cggtatattc caccagtgtg cccggggggt agggtgggcg ggttggcagc gccgtggggg
      661 taggctgatc tggcgtgggc gccgcctcgg tctgcattgg cgcaggcgtc ggcaccgccg
      721 gggtcacgca gcctgccagt gcaaccacaa gcatcagcat ccagccgacc aggcgcttat
      781 ttcgttcgaa caatgacggc ctctaccgaa ccaacggtac tggctgtaac gatcaccccg
      841 tctttgatca atagcccttc gtaatttttc tccccctgaa cggcccgcca tccgctcaac
      901 acaaacggcg ccgggctgtc ggctggcatc cactcgccat tatacttgcg cgccatatgc
      961 acatgccgcc ccgttgccga tcctccctcg caggagggat gcccaatttg ctcatcctga
     1021 tccagccact cgccaaccgg cacccgcccc atggtggcaa tatgcaggta taaaatcacc
     1081 cagcccgtct gctccgaccc gtcgccatcc agatccacta caaccagccc gttatccgag
     1141 cgggtcacca gtccgggcgc cgccgcggta atccagtaca tggacgcctc gcagcttcgt
     1201 tcgtctgatg gcggggcaaa gtcgagcgcg gccatggggg tgccggtgcc ccaggcgggg
     1261 tgcggcccgc cggtataaac ccagggcagt ccgggggcaa agggcagttc catcggcggc
     1321 tgtgagaagc ccgccggcag cagcggttca acggtctgcg cccgctgcca ggggctgtca
     1381 aacatttctt cataccgcac gctcaggctt tcggtgccgt acaccgcacc ggcccaggcc
     1441 agcggctcat agatctgact gaataaatac agcagcccca ccgtcccggc attgagttcc
     1501 ggccacagcc gccgggtttc accgttgggg aagctgaggg ttgtcaccgt gcccagccgc
     1561 cagccgtaat atcccagcga catctggcgc gccgcccagg tgagctggtg atacagcccc
     1621 ttttggctga aatctacatt acccgccgga taattggttt ctgccaggct ctgcggctgc
     1681 ccggtcaccc agtgtgattg atattcaata attgccagca gtagccgggg gttgatcgaa
     1741 ttttcttgcg ccacccgctt gaccacctgc gccccgtcat accacccatc gctcagatac
     1801 tcgcgataac tgctcaggta gccaccggct ccggcaacgt acccatccag gtcaaaatcc
     1861 agcgccgttg ccgaatacac cacctctgaa tccggcataa cgcgttccat gacctgccgg
     1921 tctggcagaa catccgggat caccagcaac tggcccgggt caataaagcc ttccgctggc
     1981 agcgcctggt tgcattcaat ttgatccacg ctcaccccga accggatcgc cagcgcattc
     2041 aatgaatcac ctgcctgcgt agtgtacagc agcggtgggc gcggcgtaat tggcacagtg
     2101 gccgttttgg tgggcgcagg cgtatatgcc aaaaactgca ccgatggcgt aatttgtggc
     2161 gtttcagcgt tgggcgtatc catcccgccg atgcccaggt catacgtcgg aacgggggta
     2221 accgcctctg gcccccaggc gcaggcaagc agcgaaatcg cccacgctgc cagcacggtc
     2281 aaagtcagcc agcgcttagc gggtaatttg attttggggt acgaaacgat cccaggcttc
     2341 aatcacaact ccatcacgtg tcaggcggcc attatactcg atcccgtcgc cggcagagac
     2401 ccagccatcc atcacaaatg gcaggtcacc gtcggccgaa atccactctc cattataccg
     2461 ccgggcgatg tgcaggtgtg ttgccagtga gtatccacct tcacaggaag ggcgcccaat
     2521 ccgatctccg gcttgcacaa tatcacccac cggcgcccgc tgccagctgt ccacgtgcag
     2581 gtagagcacc gtccacccgg tttgttccaa accgtcgccg tccaggtcta aaaccaccat
     2641 cccatggccc gagcgtacca ccacaccgtc agcaaccgcc gttacccagt tgggcgagac
     2701 cacgcagcct tgcggttcgc cggggggcgc aaaatccagc gcagcccagc ccgacccatc
     2761 cgtccatccg ccgtgcggcc cgcccgtaaa ggaccaggtc tccccaacat tgaacggcag
     2821 taccattgtt ggctgtgaca atccctctgg aatcagcggt tcaatcgcca ggtcaaatgg
     2881 atagccaaac aggttctggt aggtggcaaa caacccatct tcgctcacgg cgtgctgcca
     2941 gtctgccgcc ccataaagct gccccaggta atactgcacc ccggctgtcc ccgcgttgag
     3001 ggtcgggtca agcggcgcag cgctgccatc ggccagcgga atttgcccca gcgcttcagc
     3061 gcgccacaga taataccccc gattcaaagc attagccgcc catgacagtt gatgatacaa
     3121 ccccacccgc cagtattcca tcgttgccag cggatgcttc ttctgatctt cactggggtc
     3181 gcccccggta acccatccgc tctgatattc cagcactgcc agcagcaagc gtggattgac
     3241 tgagttttcc tgcgccacac gttccaaaac agccgcgccg ctggtctcca tctcatccac
     3301 aacttcccag tgccggctca agtacccgcc ttgctgctca acaaattctc ccacgttaaa
     3361 ataagcgctg accggcccat acaccagttc ggaatccgga ataatcttga acccggaggc
     3421 cccaatttga acctccggcg gaggaatgat cagcacttgc ccggcataca accagtctgc
     3481 cgggttgatg ccgttagcct ctgccagcgt attgacgctg acgccgtagc cctgcccgat
     3541 ccccgccagc gaatcccctg cctgaacggt gtaatagatc gtctcgctgc gctgcccggg
     3601 cagctcgcgc ggcgcatcag gtgtcggcgt cagaatgggt gctcccggca cccgcgtggg
     3661 cggcaggaag ggggttagtg tcaccgtccc ctggggaatg gtctcggatg tggcattcaa
     3721 ccaccagagt gtgctttctt tatccggctt gccacaggcc gataagagca gccccagcat
     3781 cacgaagctc atcagcacgg cagaaaggcg ttggggtttg agtctcatgc gggttgctcc
     3841 tggttgtctg gtgtatcaat gaaaatggat gggtaaatgt cgtccgccca acaaatgcaa
     3901 gtgcaggtgg taaatcgatt gtccaccatc atgcccggta ttcatcatca gccggaagcc
     3961 gctccggtca attccccgct cagccgccaa cttctgtgct actgtaatca gatgccccag
     4021 cagcgcctca tcagccgggc cgattgcatt cactgaatca atatgctggt tcggaatgat
     4081 gaggatatgc acgggcgcaa tcgggtgtgt atcttgaaaa gctgtcaccc ggtcatcttg
     4141 ataataaatt tttgccggtg cttcaccggc tgcaatctga caaaaaatac acgccattaa
     4201 tataccctga taaaagttac tgccctggcc gcaccttcaa gtgcggccag ggcagcgcat
     4261 gataatctta cgcctcgccg cggatgaagg cttcagtcat ttcgcggcac tggtcatctt
     4321 tgaactgccg gggcggcgtc ttcataaagt aagatgccgg accatacagc gggcctgcaa
     4381 tcccccggtc acgcgccagt ttgatgcagc gtactgcatc aatcaccaca cctgccgaat
     4441 tgggcgaatc ccagacttcc agcttggctt ccaggttcaa gggcacctcg ccaaaagtcg
     4501 tcccttccat ccgcaggtag caccacttgc ggtcggtcag ccacggcaca tagtcgctgg
     4561 ggccaacgtg aatattctct tcactgatcg ggtaaggcag catactggtc actgcgccgg
     4621 tctttgaaat cttcttggat tccaaccgtt cacgctccag catattcatg aaatcagtgt
     4681 tgccgccgaa gttgagctgg taggtgcgat ccatccgcac accgcggtcc acaaacaggc
     4741 tggtcagcac ccggtgcaaa atcgtggcgc ccacctggct cttgatgtcg tcgccgatga
     4801 tcggcagccc acgttcgcgg aagcgctctc cccagtattc agagctggca atgaacaccg
     4861 gaatggcgtt cacaaaccca accccagctt ccagaatctg ctccacgtac cacttggtcg
     4921 ccatttcaga acccacgggc aggtagctca ccaccacatc cgtaccggta tctttaagga
     4981 tctgaacgat attggcggtt gggcccgggg ccttcttcag gatctgcgaa aggtacttgc
     5041 ccagaccgtc atgcgtcatg ccccgctcaa cccgcacacc cagccgcggc acatcacaga
     5101 acttgtaggt attgttgggg tatgaaaaaa tcgcctcaga gaggtctttt cctaccttgg
     5161 tatccacaac gtcaaacgcc gcgctgaatt caatatcgcg gatgtgataa ccgcccaggt
     5221 tgacgtgcat caatccagga acgcggtcgg tatcggctgc attgccataa aaatgcacgc
     5281 cttgcaccag cgaagaggcg cagtttccca cgccaataat tgccactctt acctttttgt
     5341 ctgccatagt gtcttgctcc tttagaaagc cttctccttg attggaatat gttcgtgccg
     5401 cctatgctat aatcaaaccg aggagcttgt tgtgcagcat gggcgtggac accattgatt
     5461 atactcgccg tctcactcgt ttgcttgaag tttgtaaaaa tttaagctcg aatcttgagc
     5521 ttgaaccgct tttacaatcc ctgctcgagg tggcctctga gctgacccac agcgaaagca
     5581 gctccattct gacctatgat gaagaaaacg gcctgctcaa attcctggcg gttccctggc
     5641 acctcaaaga gcgctccagc gccatcggtg ttccgctgga tgccagcgcc gcaggctggg
     5701 tctttcttaa tcgccagccc atgctggttc aagatgccca ggacgaccgc cggattttcc
     5761 gggctgtaga tcttgaatgg aacaaccaaa cccgctcact gctggctgtg ccgctttgtt
     5821 ttcgcggcca aaccatcggc gtctttgaat gtaccaacaa ggcccagcag gctaattata
     5881 ccgaagaaga tgtttttata ctggaaacac ttgcggcgca ggcagcaatt gccatccgca
     5941 accgtcactt actcgagcag tctcgccagg cgtatgagaa agccctggaa agcgatcgcc
     6001 tgaagggcga ttttatcgcc atcatgtctc acgaactgcg cacccccctg ggggttgtgc
     6061 tggggcattc cgccctcctg cgcgatatgg cgcccgaaaa tctgcgcgtc gacctcgatg
     6121 tgattgccaa atcggccatg cgcctcaaag aaatcataga agatttttcc aatgtggacc
     6181 gtattgaagc cggtcttgcc tcactgcgcc gccagcgggt catgatctcc actttggtgc
     6241 agagcgtggt cgattctttc aaagacctgg cccgtgacca tcaaatcgct ctggatttcc
     6301 acgtctcaca gcgcaacctt gctctcgagg gtgatgaatc caaaatcgcc attgccctca
     6361 gcaacctggt gcgcaatgcg ctcaccttca ccaatcccgg tgggcaggtg caggttcgtg
     6421 cagaacaggt gcctggctat atcaaaatcg ccgtcattga tgatggtatc ggcattcccg
     6481 cagccgaaca ggaaaagata ttcgagcgtt tttatcaggt tgaaaaacac ctgacgcgcc
     6541 ggcacggcgg catggggtta ggcctttcca tcgcgcgtga atttatcgaa ctgcacggcg
     6601 ggcgcatctg ggttgaaagc accgaaggca agggcagccg cttcacattc ctcttgccca
     6661 aaagccaggc cgaagccgcc gccgccgggc gcgtatttgg cagctagcgc cgcgtcagat
     6721 gtttgtgcac tggcgtttgc cgccagggcg aaacatggtg caccggtact gagatagggg
     6781 gcatattcca ggctgctcgg gcgtggttgc agcgctccag gtcccctggt cgaatggaca
     6841 tctgcccgcc ctgccactgc accccaaaat cgcggcaggg gctgggccgc tgctcgtaga
     6901 tggtgcagcg cgtgggcccg cccggcttgc cttccagcgc aatgcaccgc cgtgaaagct
     6961 gattggtccc gcgcatggcg cagtaatatt cagtcacatt ttctgtcaat tcaaccggca
     7021 cctgcccacc ttgcgccggg tcggtttctc cccagtagaa cgatacgcga aagaacgcac
     7081 agcacgcccc gcagttcagg caggggtttg cgtgaagctc accatgcagt gttattttct
     7141 tcatcggtta tctggagata tctgccgcgc cgcccactcg gtaactggct tatccgcagc
     7201 ctttcaatac aaagtcgtag tttttagagt tggtcacgtc gcggttcata tccacaacaa
     7261 taaactgaat ctccagcagt ccgctccagg aacccccata agtgctgaag cgatctgcaa
     7321 tccagccatt gagacccatc tgccggagcg cgggcatcgt catggtatag tggtcgccgg
     7381 tttgtttttc catcaaaatc ggcaccagcg cccaatcggt catatcaccg gtgccctggt
     7441 caacaatccg gtaatagaca ttgatcaggt aaatgccatc cgggtcagtc acatccatct
     7501 caatccccag ttgatcattc gcgcaaccgg catagtaact gacggtggtt gtagtgaagg
     7561 tcggctcgcc cagggctgct tttgccgggg tagggctggg tgtcttgctc ggcgtctgcg
     7621 tgatggtcgg ggtaggggtg atggtgggcg tctgtgtgat ggtcggtgtg cgtgtgatgg
     7681 tggccgtggc gctcggcttg agtgtggctg tagccgtggc ggtcaggctg ggtctgggtg
     7741 ttgctgtagg cttcggcgtt gatgtgaccg tcgccgtata ggtagcggtc ggtctggcaa
     7801 acagcgccgg cagttgaaaa tcgccccctc tagagaaata ttggctgagc cccacaatca
     7861 acgctgcggc aatcaacacc gtaaccacca gcccgccccc cacaaaccac gcccagcggg
     7921 ggatgggttt gcgggctttc ttggggcgcg ccgccggggc ctccacctga gcccgctcca
     7981 ccagggttgg cgcactcagg cttgcagccg ttttaagcgc tggctcaatc acagccggcg
     8041 ggtccatcac cgggatggtc ggggcggctt ccggcacctc aactgccgct gcctgcacgg
     8101 gttctgtcgc accgccgggc tgcatcagca agccctccag cacggagata aacccgttca
     8161 tgtctgcgta ccgatcattg ggttgttttt gcagcgctcg cgccagagcc gtaaccacgg
     8221 cttgcggcag gctgggcaca aactgtcgcg ggtcaggcag cggttcagtg atgtgtttga
     8281 gcatcaccgc catcggcgtg tcagcctggt agggcgtccg cccggtgagc agttcataca
     8341 gtaccacgcc cagcgcatac acatcggcgc gcccatccac cggatttccc attgcctgtt
     8401 cgggcgccat atattccggc gttccaatcc ccgtaccagc gccggtcaat gttgccccac
     8461 tgctcaaatc cagaatcttg gcaatcccaa aatccatcag catcggctcg ccgctctcgg
     8521 tcattaaaat attggagggc ttgatatctc gatgcacaat attttgcgca tgcgcatagc
     8581 tgagggcgcg tgccaccggc aaaaccagcc ggatggcctc ctgccagggt atcggtttgc
     8641 cggtccgctg ccgcagggtg ccgcccggca catactgcat cacaaagtac ggcgcacctt
     8701 catattctcc ataatcatgc acattgacaa tgtttggatg cgtcaatcgc gccagcgcct
     8761 tggcctcacg ctcaaaccgc gccagcacct gctccagcag ctcagtggta aaagcatcct
     8821 tgcgaataaa cttgattgcc accatccggt ccagccgcgt atccagggcg cgatagacag
     8881 ttgccatgcc gccttcgccc aattgcgctt caatttgata tcgcccaatt gtctgaccga
     8941 gtaaattcat aaggttgtcc agccggggat gattatctca attatatgcg aaaacccaga
     9001 cttgatgagg gacatccatg ccgtgtttct cgggctgtca ctctggtttc tgcgcgtcag
     9061 gcgtcaacgc cgcgcctgcg gctgaagcct ggatgacctt ttctcgcatc gccgccagac
     9121 cggccgcggc cagggctaaa cccgccccaa ccagcagccc ggcttgcaca gcgctgaata
     9181 gggtgtctgc ttctgccggt cggtagatgg aagtgagaat ggcgccagcc aatccaatgc
     9241 ccagcaccat acccacattg cgagccgttg ccagcatccc ggcggcaatc cctcgccggt
     9301 gccccggcgc tgaccccatc aatgcgctgt tgttgggcga ggcaaataag gccataccca
     9361 ggccaaccac agccaatccg gcaataattt gtcctgtagg ggagctggct cccacaaagc
     9421 tgagcattac catcccgccg ctgacagtca gcatgcccag cgcggtcagc aggcgcgacc
     9481 caattttgtc cgacagactg ccgctgattg gcgcaatcag ggccatcaca atcggctggg
     9541 cagtcaggat caacccggcc tgtgcgggtg aaaaatcgcg cccgtgaatc agataatagg
     9601 gcagcaggaa aatcaccgca tacaggcaca tatatgataa cagcgccgaa ctgaccgccg
     9661 tgctgaatgt ccggctgcgg aatagcccca gatccagcat cggtgcaggt tgctttcgtt
     9721 cccaggccat aaagcccacc agcagtccaa ccgctgtgcc cagcagcccc agcgtcggca
     9781 gtgagcgcca tccccaggcc gccccctgat tcaatcccag cagcagcgcc accagaccga
     9841 ccccaaagag cgctgcgcca accagatcaa accgttcctt gccatccgtc tttcggtcag
     9901 ccgggataaa gcgcaggctc aaggccagcg ccagcagccc aaccggaatg ttaatgtaaa
     9961 acacggcccg ccatgaaaac tgctccgtca accagccgcc cagcgatggc cccaccgtca
    10021 aacccaggta ggtcattgtg gcttgcagcc ccagcgcccg acctcgctgc tgggccggaa
    10081 acactttggt caaaatggcc ggcgaattgg caaataacat cgctgcgcca atggcttgca
    10141 gcgcccgcgc cagaatcaat cccgtggggc tgccagccag tccacataac gccgaaccca
    10201 gcagaaaaat cacaaacccc agcgtataga cccgcttgtt gccattcata tcccccagcc
    10261 gccccaccgt cagcagcagg ctgctaacca ccagtaaata gaccgtcacc acccattcaa
    10321 tcgtcgcaat attgctgtca aagctggtct gcagcaccgg taaaatcgta ttgaccacgc
    10381 tgccatccag tgccgacata aacgtcccca gcccaatgga gaccagcacc caccattttc
    10441 gaggcgaatt ttcagtattc tctagcggca gcatcagaca tccttacgca aacgggttgg
    10501 ccgtggcggg cggcggttca agattatatc gcgaattcag cccctatcca tccccacctg
    10561 ctgtcaattt tccgaaccgc ttcgcgtcgt ccgcgtgctg ggcttgacac atcctcaaaa
    10621 ccttgataaa ctgacaaccg ttaaggaaac ctaatgaatg gcgttttact ctggaatggt
    10681 gattaatcag gctctttgcc ctcgcaccgg gggcgggtct tttaccttct cggagtgcgc
    10741 ctgctgactg ttttgtacaa tgtataatgt atcaacggcc acggcacacc ccagcctgtg
    10801 gccgttttga ttttatcgct taagctgttt gtttcttttt ggaggtcgtt catgtcactc
    10861 atcactactg aaaatctggg gttatccttc ggtgcttttg atttgttcct gggcatcacc
    10921 gtccatgtac ccaacgatgg caaaattggc ctcattggcc ccaacggaat tggtaaaacc
    10981 tctctggtcc gtatccttgc cgggttagat caacccaccg ccggccgggt ctatctggcc
    11041 cgcggccgcc gcattggcta tctgcgtcag gaggcagtgg atgcctttgc tgaccgcacc
    11101 aacaccgttt acgaagaaat gctcaccgct tttgaagatt tgcgcgccca acagacccgc
    11161 ctcaacgagc tggaagcccg catgtccgcc ggagatcacg ccgaagcgct gctgcagcag
    11221 tacggtgatc tgcagcacgc ctttgagcaa aacggtggct acaactacga ggttgatatt
    11281 cagcagacgc tggaaggcct ggaccttggc aaaaattact ggcacatgcc cctggcgcat
    11341 ctcagcggcg gacagaaaac ccgcgccctg ctggcccgcc tgctgctcga aaaacccgac
    11401 ctgctcattc tggatgagcc caccaaccat cttgataccc aggccgttga atggctggag
    11461 cataccctcg gtgattggcg cggcgccgtt ctgatcgtca gccacgaccg ctatttcctg
    11521 gataacaccg tcgataccat ctgggagatg agccgggctg gcatcgaaac ctactcaggc
    11581 aactattcgt catatcttgt ccagcgtgaa gaacgctggg agtattacga gaaggtcttt
    11641 gaagaagaaa aaacgcgcct cttgaatgag gtggattata tccagcggaa ctgggtgcga
    11701 gatagcaccc atgctcaggc gctgggtcgc ttgcgccgcc tgagccgtga catcgccatt
    11761 gtcgaaaatt ttggcctgat ggcgctgcgc agcggcaaga aatggtctga aatggacctg
    11821 cacgtcgagc gtccattaga cgtcattgaa gccatccgcg cagtcaatgc cattcaaatg
    11881 cctggcaacc gccccaccca catccgcccc cgccttgcct ctgacaaggt cagcggctcc
    11941 atcatcctcc gtgctcaaga cgcagccatc ggttatcccg gcaataacct cttcaacatc
    12001 cgtgaggttg aactccgccg cggcgaatgt gccgccctgc tcggccccaa tggcagcggc
    12061 aaaaccacct tcctgaaagt gcttctcgga cagttggacc ccctcagtgg cagtgtcaac
    12121 ctgggtgcca gcttgcaaat tggctacttc gcccagtcac acgacgcgct aaaaggtgac
    12181 cacaacatcc tggatgagct tgcccgccac aaaccccttg agatggagca ggctcgcagc
    12241 catctggcgc agtacctttt ccggggtgat gatgtcttca aacagctctc ctccctcagc
    12301 ggcggcgaac gggcccggct ggcactggcg atcctggcgc tggacggcgc caatttcctg
    12361 ctgctggatg agcccaccaa ccatctggat atgcccgccc gcgaagcgct tcaagatgtt
    12421 ctggacagct tccccggcac cattctgctg gtctcgcacg accgttatct ggtggaccaa
    12481 ctggcaactc agatctggga aatccgagat ggcgccctga acgttttcaa aggcagctat
    12541 cgcgaatttg ttttgcggcg cgcaactgcg ccgggggccg ccgctcggac tatcgtcatg
    12601 ccgcccaagc ccatcatgcg cggcaatgat aaagaagaac gccgccgggt tcaggccctg
    12661 aacctgttag aagaacgcat ccacgagcag gaaacccgca tccagcgcct ttccagcgaa
    12721 ctgcagcgtg ccgggcaatc ccaggctttt gagcgcatcc aaaaactcag ccgggaaatt
    12781 ggccaggctc agaccacgct ggaagaactg atttctgaat gggaaaagct ggcagcctga
    12841 ccatgcccga ccaccagacc atctatcagc aagctgccgg gcgttatgac cgcctggtaa
    12901 gctgcgaaga tgccgccggg aacctcctga tcgaactgaa taagatcacg tcttttgagg
    12961 gtcagcagat tgtcgagttg ggcgccggca ccggccgcct gactcgtctg ctggcccctc
    13021 tggcgcgcac cattcacgcc tttgacctgt cgccccacat gctcgccttt gccgcacagc
    13081 gcctgaaagg gctgtctatc caaaactgta ccctggctgc ggccgagcat cgtgccatcc
    13141 ccttgcctgc cgcctcagcc gatgtcgtta tctctggctg gagcctgtgc tatctggcgc
    13201 agggcgaccg ccgtatttgg cagccggttg ttcagcaggt tttagacgaa acctaccgcc
    13261 tgctccggcc gcatggctgg cttatcctga ttgagaccct cggcacaggt tttgaaaccc
    13321 ccaccgcccc cgaaaaactg ctcccctatt acgatttcct ggaagctcaa ggctgtgccc
    13381 agacctggac ccgcaccgac taccgctttg atacagtcgc cgaggccgtt gacctgaccc
    13441 gcttcttctt cggtgatgaa cttgccgatc agcttgaacg ca
//
LOCUS       DAOPVS010000050        12769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_5006_length_12769_cov_58.395234, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000050 DAOPVS010000000
VERSION     DAOPVS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_12769_cov_58.395234"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKL11_05860"
     CDS             <1..222
                     /locus_tag="PKL11_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13164.1"
                     /translation="APVVSPGNLIRIMRQQTSNRIFTQSASLKEQNPSGDFWAPGYLV
                     VSGSQPPGGKLVREFVRRIRRHQGFDQSG"
     gene            226..3009
                     /gene="polA"
                     /locus_tag="PKL11_05865"
     CDS             226..3009
                     /gene="polA"
                     /locus_tag="PKL11_05865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNZ13165.1"
                     /translation="MPPVLYLIDGHALAYRAYFALTMSASERMQTRTGEPTAGIFGFA
                     SVILRLLEQDHPEYLAVAFDTGKTFRNDLFPEYKATRAKMPDDLRTQIDRIRQMLDAF
                     QIPRLEVEGVEADDVLGSVAQQAVAHGLGVKIITGDRDLLQLVNDRIIINLAGSKMSE
                     SRDFTAELVVDSLGVRPDQVVDYKALVGDKSDNIPGVPGVGEKTATALLAQYETLDTI
                     YEHLAEIPDRVRNKLESGRDLAYLSRNLATIRTDVPVSIDLEQAHTTRLDLVAAEAIF
                     REFEFRSLINRLRALAPAAAPAAGQQLSLFGEPVQRIGAPTPTSLNVHIVRTPTDLDE
                     LVKALSGAEWISLDTETTSIDPMLADLVGISLAVQPGEGYYIPLGHHQDDNQLPLEQA
                     LAALRPALTDPAKPKMGHHLKYDVIVLAEHGLLVSPLSFDTLLAEWVVNPSSRALGLK
                     DMAYDYLGQSMTHIEELIGSGKNQISMADVPVEQVAPYAAADAEVPLRLRPLLQSRLD
                     EFSATSLFQTIEMPLVAVLVMMERAGIALDSTFLEKMSVELQTRMGEIEREISRSVGY
                     AFNLNSTQQLSKVLFETLHLEPPDRRKKTASGHYSTSADVLEELRGRHPVVDWILEYR
                     ELAKLRSTYLEALPKQVNPRTGRVHTSYSQTAAVTGRLSSSNPNLQNIPTRTELGRKV
                     RAAFVSDPGNVLLSVDYSQIELRIVAAMAGDEAMLAAFRAGQDIHATTAAAIYQVPLA
                     EVTKEQRRHAKAINFGLIYGMSAFGLSRTTGLTLAESENFVRAYFERFPGVKNFLDGL
                     RRQASQRGYVETLLGRRRYFPNLQTLTDANQRNREEREAINAPIQGTAADIMKLAMIH
                     LPSALTAAGLHANILLQVHDELVLECPADELKATARVVQQVMEDAYALVIPLTTEARW
                     GQNWGDMQVLHSS"
     gene            3023..5341
                     /locus_tag="PKL11_05870"
     CDS             3023..5341
                     /locus_tag="PKL11_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ13166.1"
                     /translation="MGDPEAFQKAMNLGHDSAWDQDWEHAAQYYRQALDLMPDNVQAL
                     TSLALALFEMQDFEGALSAYQQASFRSPEDPIPVEKMARIYERMGRLAEAIKASLQAA
                     DLFLKAREVEKALQNWTQVLSLQPENATARSRLAVVYEKFGRKPEAAAEYLALASLLQ
                     RSGSVANAAQAITRALQLAPESPEVNQAVAMLRTGQMLPPPPRPRGGTAPMRMAAARQ
                     NTLAPAVDEPLMTPVAEARKRALVQIAALLFEQSESMAQNEVQSQRRGFGSLTRGTGG
                     LTMDQTDQTRTLLHLSQAIESQTNGQDAQAAAELQKAVDIGLSHHAVYFDLGMTLIPD
                     DPQRALKYLQKAVRNPDFALAANFLLGQIYQATENLPQAVAHYLQALRLADMETVLPE
                     QADEVGQLYETIVETQSQKTDTAELTTICETVLKQLDRDDWRKFLRTARAQLPPQPAG
                     SPPMPLAEMLLESRSGQVIEALARVRQLSDQGKIQSAMEEAFYALRFAPTYMPLHVQI
                     GELLIKEGRVQDAVEKFMLVAQLYSLRGDASAAIRLLSRVTQMAPMDLSIRGRLIELL
                     TAQGRVDEAIQQYIDLAEIYNSLAELDMTRQTYQSALRLTQRSSNPRKWSVDILTRVA
                     DIDMQRLDLRQAARIFEQLRTLQPEASEPRRQLIALNFRLGQESAALTELDGYLSLLE
                     NSSKRTDAITFVSQLRDELPDQIEIRKRLADLHLRNEDKPAAIAEMDSMADMLHASGN
                     RLGAITMVRAIIALHPDNVEQYQTVLDQLQNG"
     gene            complement(5420..5492)
                     /locus_tag="PKL11_05875"
     tRNA            complement(5420..5492)
                     /locus_tag="PKL11_05875"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5457..5459),aa:Phe,seq:gaa)
     gene            complement(5529..>5774)
                     /locus_tag="PKL11_05880"
     CDS             complement(5529..>5774)
                     /locus_tag="PKL11_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13167.1"
                     /translation="PTLEEELPNFWEIGSAMQGFAVQVRPPTLPQPVLQRLGEPDVNS
                     PIRLREQLQETYEAISAAAISLAFGDFGPGESGEENA"
     assembly_gap    5775..5874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6059..8971)
                     /locus_tag="PKL11_05885"
     CDS             complement(6059..8971)
                     /locus_tag="PKL11_05885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ13168.1"
                     /translation="MVLRLPTSRIGPLPSPQLNHNWDVDSLTSPYLSPWEIGGLWLPA
                     RKAFSLLVNVPLEIREIGFSLGIDARYWRTVCNLVLEVLADQKLLPVLVPSNHHQTSY
                     IARWQPVLDGPQDGPRLAKLVSAMPPICRAETRGERLQGIEPPRGLLDSFLNTMCDAL
                     ARTWGRTRNQRLLASSEHPLERWLASLFTDDPTVTNASPAQLQALSSSLQAWMRNLRA
                     AGDQHFRIAFQLEAPEIQPGDANQPAWTLHYLLQSRDDPSLIVPAEEVWRLRGNVLTQ
                     LGKRFEQPQEKLLAGLGYAARLFPPLLTSLQAKQPTGVELSTNEAYTFLRQAAPLLEQ
                     AGFGLLAPPWWNQKGSRLGVRLHLSPRGAATPVSAGKLKLDTLISYEWQVSLGGTELT
                     REEFEILARLKLPLVQIRGQWVQLDTEQIEAAIKFYESQRQRGEMGLMDAARLTLDED
                     SAVQGLPLEEVIAEGWVSEWLEHLSHQDRMVELPQPGGLQGELRPYQRYGFSWLAFFR
                     RWGLGACLADDMGLGKTIQALALLLYEKESLGKLPGPTLLVCPTSVVTNWEREARRFA
                     PSLKVLAHQGPDRLRNSEFKDAARNSDLVLTSYALLRLDAELMQSIAWYGVILDEAQN
                     IKNPAAKQTQAARQLTAGFRLALTGTPVENRLSELWSIMQFLNPGFLGTQAGFRREFS
                     LPIERFADPEATERLRKMVSPFILRRVKTDPNVISDLPEKIEMKEYCHLSEEQATLYQ
                     AAVQETMKKVEESEGIQRRGLVLSLLTQLKQICNHPGQFLHEEPETIAEAINGRSGKL
                     ARLIEMLEEVLETGERALIFTQYAEMGHVLAEALPRALGAGVLYLHGGTPAKMRDQMV
                     HRFQEEDHGPSLFILSLKAGGTGLNLTRASHVFHFDRWWNPAVEDQATDRSYRIGQLK
                     NVAVHKFITVGTLEERVDEMIESKKGLVDSVIGAGEDWMTELSTEELRDLVALRIS"
     gene            9553..9924
                     /locus_tag="PKL11_05890"
     CDS             9553..9924
                     /locus_tag="PKL11_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNZ13169.1"
                     /translation="MTKSRTQMMVERLRELQASSPDIEASAIVSVDGLTIASALPQGV
                     EEDRVSAMSAAMLSLGERIAGELGRGSLEQVYIKGQKGYVVLMSVGQEAVLTALAREQ
                     AKLGLIFLDMRRAAEDLTKYI"
     gene            9939..10571
                     /locus_tag="PKL11_05895"
     CDS             9939..10571
                     /locus_tag="PKL11_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNZ13170.1"
                     /translation="MEPIPKSGLFYPNKISRIALLALEDVMGKNGVNAILNLASLPHL
                     IGNYPPTDLNREFDFADFSAINGALEEMYGPRGGRGLALRAGRATFDEGLRNFGALAG
                     VGDLAFKVLPLQAKLRIGLPAMARIFSQTSDQLSTVEEKDDHFVYTIHRCPVCYGRHT
                     DKASCFIAVGLLQSGLKWVSGGNEFRVNESKCMAVGDSVCEFIVYKEPIS"
     gene            10583..11446
                     /locus_tag="PKL11_05900"
     CDS             10583..11446
                     /locus_tag="PKL11_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ13171.1"
                     /translation="MTSEKKETLLIVEDDLDIADMLNAYFRVQGYEVITVNWGEDGVS
                     TCQNTPPDLVILDIRLPDIDGFEVARQLRSNRRTQSIPIIFLTEKRDRVDRLTGLELR
                     AEDYITKPFDITELRLRVRNALRRAQQGSLTNPVTGMPDGSLVDEHLMQFLQSPDRAL
                     LLVSLANLDHFREVYGFVASDDLLRAVSLMMQDTVRELCAPDDFLGHMDTSTFLIYTR
                     PANLPALKERIRIKLQQSFEYFYSTRDREGGTFEGEKGLSVQLHELTRKSQVFRDLAE
                     LKETLQNLCRS"
     assembly_gap    11522..11621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11622..12038
                     /locus_tag="PKL11_05905"
     CDS             <11622..12038
                     /locus_tag="PKL11_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNZ13172.1"
                     /translation="ILELVQTLANCDMAVGSRYVPGGSLDERWPAWRRGLSAFGNWYA
                     RTILGLPMRDVTGGFRLWRNETLRAMPLDRVRSNGYVFQVEMAYVAHRLGFTFREIPI
                     HFADRRWGKSKMSLRIQAEAALRVWALLGRYRNLKP"
     gene            complement(12035..>12769)
                     /locus_tag="PKL11_05910"
     CDS             complement(12035..>12769)
                     /locus_tag="PKL11_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13173.1"
                     /translation="GSSIPANPDLNPGTMALQVLFAQWFNETGWREVLYGQASFSIDY
                     TQFAGDPWSQAASPIIPEGLIAPLLELPFEPGVLWHFTGGPHVAWGVFSPLGAVDFAP
                     HAEVKGCTPSAEWITASAPGLVVRSDNGAVMVDLDGDGYEQTGWVIFYLHVATADRVP
                     VGTWLAQDDRIGHASCEGGMATGRHVHLVRKYNGEWIAAAGPFPLVLSGWQIIPGEQA
                     YVGTLERDGVVIPSRGTGSILNQIVR"
BASE COUNT         2620 a         3955 c         3250 g         2744 t
ORIGIN      
        1 gctccggttg tctcacctgg aaacctcatc cgcatcatgc gccagcaaac ctcaaaccgt
       61 atctttaccc aatccgcttc actcaaagaa caaaacccct ccggcgattt ctgggctccc
      121 ggatacctgg ttgtcagtgg ctcgcagccc cccggcggaa aacttgtccg tgaatttgtc
      181 cgccgcattc gccgccacca gggttttgat cagtcaggat aacccatgcc gcctgtttta
      241 tatttgatcg atggacacgc tctggcgtac cgggcttatt ttgccctgac catgtcggcc
      301 tctgaacgca tgcaaacccg caccggcgaa ccaaccgccg gtatctttgg ctttgccagc
      361 gtcatattgc gccttttgga acaggatcac cccgaatatc tggcggttgc ttttgatacc
      421 ggcaagacct tccgcaatga tctcttccct gagtacaagg ccacccgcgc caaaatgccc
      481 gatgacctgc gcacccagat cgaccgcatc cgccagatgc tggatgcttt ccagatcccc
      541 cgccttgaag tcgaaggtgt agaagccgat gatgtcctgg gcagcgtagc tcagcaggct
      601 gtagcccatg gtctgggcgt caaaatcatc accggtgacc gcgatctgtt gcagttggtc
      661 aatgaccgca tcatcatcaa cctggctggc agcaaaatgt ctgagtcgcg cgattttacc
      721 gccgaactgg ttgtcgattc cctcggcgtc cgccccgatc aggtggtgga ttacaaagcc
      781 ctggtcggcg acaaatcgga taacatcccc ggcgtgccgg gtgttggcga aaagaccgcc
      841 accgcgcttc ttgcacagta cgaaaccctg gataccattt acgaacacct tgccgaaatc
      901 cccgaccggg tgcgcaataa actcgaatcc gggcgtgacc ttgcctatct cagccgtaat
      961 ctggcaacca tccgcaccga tgtccccgta tccattgatc tggagcaggc ccacaccacc
     1021 cggctggatc tggtcgccgc cgaagccatc ttccgcgaat ttgaatttcg ctcgctgatt
     1081 aaccgcctgc gggctctggc gccggcagcc gcccctgccg ccgggcagca gctttcgctg
     1141 tttggcgaac ccgtgcagcg catcggagcc cccacgccca ccagcctcaa tgttcatatc
     1201 gtccgtaccc cgaccgatct tgatgagttg gtcaaagccc tgtctggcgc cgaatggatt
     1261 tcgctggaca ccgaaaccac ctccattgac cccatgctgg cagacctggt tggcatctcc
     1321 ctggcggttc aacccggcga gggttattac attccgctgg gccaccatca ggacgataac
     1381 caactgcctt tggagcaggc tctggctgcc ctgcgcccgg cattaaccga tcccgccaaa
     1441 cccaaaatgg gccatcatct gaagtatgac gtgattgtcc tggctgaaca tggccttctg
     1501 gtcagccctc tgagctttga caccctcctg gctgaatggg ttgtcaatcc atcctcgcgc
     1561 gccctgggcc tcaaagatat ggcttacgat tatctgggcc aatccatgac ccacattgag
     1621 gaactgatcg gctcgggtaa aaaccagatc agcatggcgg atgttcctgt ggagcaggta
     1681 gctccctacg ccgcggccga tgccgaagtg ccgctgcgtt tgcgcccgtt gctccaatcc
     1741 cgcctggatg agttttctgc cacgtccctg ttccaaacca ttgaaatgcc cctggtcgct
     1801 gttctggtga tgatggaacg cgccggcatt gccctggatt cgaccttcct ggaaaaaatg
     1861 tcagtggaat tgcaaacccg catgggcgag attgaacgtg aaatctcgcg cagtgttggc
     1921 tatgccttta atctcaattc cacgcagcaa ctctccaaag tcctctttga aacgctgcat
     1981 ctcgagccgc cagatcgccg caagaagacc gccagcggcc attattccac ctcggcagat
     2041 gtgcttgagg agctgcgcgg ccgtcacccg gtggtggatt ggatcctgga ataccgcgag
     2101 cttgccaagc tccgctcaac ctacctggag gccctgccaa agcaggtgaa tccacgtacc
     2161 gggcgcgtgc acacttcgta tagtcaaacg gccgcagtca ctggccggct ttcctcctcc
     2221 aatcccaatt tgcaaaacat ccccacgcgc accgaactgg ggcgtaaagt ccgggctgct
     2281 ttcgtatctg accccggcaa tgttctgctc tcggtggatt attctcaaat tgaactgcgc
     2341 attgttgctg ccatggcagg ggatgaagcc atgctggctg ccttccgtgc cgggcaggat
     2401 attcacgcca ccacggcagc cgccatctat caggttcccc tggcagaggt caccaaagag
     2461 cagcgccgcc atgccaaagc gatcaatttt ggcctgattt acggcatgag cgcttttgga
     2521 ttgagccgca ccaccggact gaccctggca gaatctgaaa attttgtccg cgcctatttt
     2581 gagcgattcc ccggcgtcaa aaatttcctc gatggccttc gccgtcaggc ctctcagcgt
     2641 ggctatgtcg aaaccttgct ggggcggcgg cgttatttcc ccaatctcca aacccttact
     2701 gatgccaatc agcgcaaccg tgaagaacgc gaagccatta atgctcccat tcagggcacg
     2761 gctgctgata taatgaagct agccatgatt catctgccgt ctgctctgac ggcggctgga
     2821 ctgcacgcca acatcctgct ccaggtgcac gacgaactgg tgctggagtg tcccgcagat
     2881 gagttgaaag ccaccgcccg ggtggtccag caggtcatgg aagatgctta tgcactggtt
     2941 atccccttga ccaccgaagc acgctggggg cagaactggg gcgacatgca agtcctacac
     3001 tcctcataag gaaggccaga agatgggcga tcctgaagca tttcaaaaag cgatgaattt
     3061 aggccacgac tccgcctggg atcaggactg ggaacacgcc gcgcagtatt accggcaagc
     3121 gttagattta atgcccgaca atgtccaggc gctcaccagt ttggcgctgg cattatttga
     3181 aatgcaggat tttgaaggcg ccctcagtgc ttatcagcag gcttccttcc gcagccccga
     3241 agaccccatc ccagttgaga aaatggcccg aatttacgag cgcatggggc ggctggcaga
     3301 agccatcaaa gccagcctcc aggctgccga ccttttcctt aaagcgcggg aagtagaaaa
     3361 agctctccaa aattggaccc aggtcctgag ccttcagccc gaaaatgcca cggcgcgcag
     3421 tcgtctggca gttgtctatg aaaaatttgg ccgcaaaccc gaagccgctg cagaatatct
     3481 ggcgcttgcc agtctcttgc agcgctccgg cagcgtagcc aatgctgccc aggccatcac
     3541 ccgcgccctc cagcttgccc ccgaaagccc ggaggtcaat caggcggttg ccatgctccg
     3601 caccgggcaa atgctgcccc cgccgcctcg gccgcgcggt ggcaccgccc ccatgcggat
     3661 ggctgcggcc cgtcaaaata cgctggcccc cgcggttgat gaacctttga tgacccccgt
     3721 cgccgaagcc cgcaaacgtg cgcttgttca gattgccgca ctgctctttg agcagtccga
     3781 atcaatggct caaaatgaag tgcagagcca gcgccgcggc tttggctcct tgacgcgcgg
     3841 cacaggcggc ttgacgatgg atcaaaccga ccagacccgt accctcctgc atctcagtca
     3901 ggcgattgaa tcacagacca acgggcaaga tgcccaggcc gctgctgaac tgcaaaaagc
     3961 agtcgatatt ggcctcagcc accatgctgt ctattttgac ctgggcatga ccctgatccc
     4021 cgatgacccc cagcgcgcct taaaatatct tcaaaaggca gttcgcaacc ccgatttcgc
     4081 cctggctgcc aatttccttc tggggcagat ctaccaggcg accgaaaatc ttcctcaggc
     4141 ggtggcacat tacctgcaag ccctccgcct ggcagatatg gaaaccgttc tgcccgaaca
     4201 ggcagatgag gtaggccagc tttacgaaac cattgtcgaa actcagtctc aaaagaccga
     4261 taccgctgaa ttgaccacta tctgcgagac ggttctcaag cagttggacc gtgacgactg
     4321 gcgaaaattc ctgcgcacag cccgcgccca actgccgccg cagcctgccg gcagcccgcc
     4381 catgccgctg gctgaaatgc tgctcgagtc ccgttccggt caggtgatcg aagccctggc
     4441 gcgcgtccgt cagctttccg atcagggcaa aattcaatcc gccatggaag aagcttttta
     4501 tgctctgcgc tttgcgccga cctacatgcc tctccatgtg cagattggcg aattgctgat
     4561 caaagaaggc cgggtgcaag acgccgtgga aaaatttatg ctggtggctc aactgtacag
     4621 cctgcgcggg gatgcttcgg cggccatccg tctgctctcc cgcgttaccc agatggcccc
     4681 catggatctg tccatccgtg gccgtctcat tgaattgctg acggcccagg gccgggtgga
     4741 cgaagccatc caacagtaca ttgacctggc tgagatttat aacagtctcg ccgaactgga
     4801 tatgactcgc cagacctacc agtctgcact gcgtctgacc cagcgttctt caaatccgcg
     4861 caaatggtcg gtggacattc tgacccgcgt ggccgatatt gacatgcagc gcctggacct
     4921 gcgtcaggct gcccgcattt ttgagcagtt gcgcactctg cagcccgagg catccgaacc
     4981 gcgccgccag ttgattgccc tcaatttccg cctcggccag gaatctgccg ccctgaccga
     5041 actggacggc tatttgagcc tgctggaaaa ttccagcaaa cgcaccgacg ccatcacctt
     5101 tgtctctcaa ctgcgcgatg aactgcccga ccagatcgaa attcgcaagc gcctggccga
     5161 tctccatctc cgcaacgaag acaaacctgc cgccattgct gaaatggact ccatggccga
     5221 tatgctgcat gcctctggca accgcctggg ggccattacc atggtgcgcg ccattattgc
     5281 cctgcacccg gacaatgtcg agcagtatca gaccgtgctg gaccagctcc agaacggttg
     5341 attgatctca acgcttttca tccaaaataa aaccgtcggg cagctttgaa actgcccgac
     5401 ggtctcttta aaagtcctgt gccgagagtc agactcggac tgacgactcc gcgattttca
     5461 gtcgcgtgct ctaccaactg agctatctcg gcaaggttcg ccaattctat cgtgatgttg
     5521 cgcgcttgtc aagcattttc ttcgcccgat tcccccgggc caaaatcgcc aaatgccagg
     5581 ctgatggctg ctgcgctgat ggcttcgtag gtttcctgca gctgctcgcg caatcggatg
     5641 gggctgttca catcgggctc tcccagccgc tgcagcactg gctgtggcag tgtgggcggg
     5701 cgcacctgca ccgcaaaccc ctgcatggca ctgccaattt cccagaagtt tggcagttct
     5761 tcctccaggg ttggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntccacc
     5881 aaccgctcca tcgccgagat ccagcgccgt gcccaccagt tgcgggcaaa atcaccgcgc
     5941 tggctgcgag cttgaatgcc cccccgtgcc cggcgtgggc tcatgccatt tgggcgttca
     6001 tcgtcattat cgttgttgtt gttataccag gacatgacag tatctctctc gtatcgggct
     6061 atgaaatccg cagcgcaacc agatcacgca gttcttctgt gctcagttca gtcatccagt
     6121 cttcgccggc gccaatcact gaatccacca gccccttctt ggattcaatc atttcatcca
     6181 ctcgttcttc cagtgtccca acagtgataa atttatgcac tgccacgttt ttcagctgtc
     6241 caattcggta agaccggtcc gtcgcctgat cttccaccgc cggattccac cagcggtcaa
     6301 aatggaacac gtggcttgcc cgtgtcaggt tcagccctgt gccgccggct ttgagcgata
     6361 agataaagag cgacggtccg tggtcttctt cctggaagcg gtgcaccatc tgatcgcgca
     6421 tcttagccgg ggtgccgccg tgcaggtaca gcacgcccgc ccccagcgca cgcggcagcg
     6481 cctcagccag cacatgtccc atttccgcat attgagtgaa gatcaaggcg cgttcgccgg
     6541 tttccagcac ctcttccagc atttcaatca agcgcgccag cttgccgctg cgcccattaa
     6601 tcgcctctgc aatggtttcg ggttcttcgt gcaggaactg tccgggatga ttgcaaattt
     6661 gtttcaactg ggttaacagg ctcagcacca accctcgccg ttgaatgcct tcgctctctt
     6721 ccaccttctt catcgtctcc tgcactgcag cctgatacag ggttgcctgt tcttcgctga
     6781 ggtggcagta ctctttcatc tcaatcttct ctggcaaatc cgaaataacg ttcggatcgg
     6841 tcttcacgcg ccgcaaaata aacgggctga ccatcttgcg cagccgctcg gtggcttcag
     6901 ggtctgcaaa ccgctcaatc ggcaggctga attccctgcg gaaaccggcc tgggttccca
     6961 aaaatcccgg attgaggaac tgcatgatgg accataactc agacaaccga ttttccaccg
     7021 gcgtgccggt cagcgccagc cggaagccag cggttaactg ccgtgccgcc tgcgtctgtt
     7081 tggcagccgg attcttgata ttctgggctt catccagaat cactccgtac caggcgatgg
     7141 actgcatcaa ttccgcatcc aaccgcagca gcgcatagct ggtcagcacc aggtccgagt
     7201 ttctggccgc gtctttgaat tctgaatttc gcagccggtc tggcccctgg tgtgccagaa
     7261 ccttcaatga tggcgcaaac cggcgcgcct cgcgctccca gttggtcacc accgaagtcg
     7321 ggcagacaag caaggtcggc ccgggtaatt tgcccaaaga ctctttctca taaagcagca
     7381 gcgccagcgc ctgaatggtc ttccccagcc ccatatcatc tgccaggcag gcccccagcc
     7441 cccagcgtcg gaagaaggcc agccacgaaa acccatagcg ctgataaggc cgcagttcgc
     7501 cttgcaatcc acccggctgc ggcagttcca ccatacgatc ctgatgagaa agatgctcca
     7561 gccattccga gacccacccc tcggcaatca cctcttccag tggcaggccc tgaacggcgc
     7621 tgtcttcgtc cagcgtcagc cgcgccgcgt ccatcaagcc catttcgccg cgttgtcgct
     7681 gcgactcgta gaacttgatt gccgcctcaa tctgctccgt atccagttgg acccactgtc
     7741 cccgaatttg aaccagcggc agcttcaatc gggctaaaat ctcaaattcc tcacgggtca
     7801 gctctgtgcc tcccagcgac acctgccatt cataactgat cagtgtatcc agcttgagct
     7861 tgcccgccga aaccggcgtt gctgcacctc gtggactcaa gtgcagccgg acgcccagcc
     7921 gtgaaccctt ctggttccac cacggcggcg ccagcaggcc aaagccggcc tgttccagca
     7981 gtggtgccgc ctggcgcagg aaggtatagg cctcgttggt gctcagctca acccccgtcg
     8041 gctgcttggc ctgcaggctg gtcaacaacg gcgggaacag ccgggcagca tagcccagac
     8101 ctgccagcag cttctcttgc ggctgctcaa accgtttccc cagttgtgtc agcacattcc
     8161 cgcgcagccg ccagacttcc tccgctggca caatcagtga tgggtcatcg cgtgattgca
     8221 gcaggtaatg cagtgtccag gcaggttgat tggcatcgcc cggctgaatc tccggtgcct
     8281 cgagttggaa agcaatccga aaatgttgat ctccggctgc ccgcagattg cgcatccagg
     8341 cctgcaaact gcttgataaa gcctgcaact gcgccggtga cgcattcgtc accgtaggat
     8401 catccgtaaa gagcgaagcc agccaccgtt ccagcgggtg ttcgctggaa gccagcagcc
     8461 gttgattgcg tgtccgtccc caggtgcgcg ccagcgcgtc gcacatggta ttcaaaaagc
     8521 tgtccagcag cccacgtggc ggctcaatgc cttgcaggcg ctcgccgcgt gtctccgccc
     8581 ggcaaattgg cggcatggcc gaaaccagct ttgccagccg cggcccatct tgcggcccat
     8641 ccagtaccgg ctgccagcgg gcaatatagc tggtctgatg atgattgctc ggcacaagta
     8701 ccggcagtaa tttctggtct gccagcacct ccagcaccag gttgcaaacc gttcgccagt
     8761 aacgcgcatc aatccccagc gaaaaaccaa tctcgcgaat ttccagcggc acattcacca
     8821 gcaggctgaa cgctttccgg gctggcagcc acaacccgcc aatctcccag ggtgagagat
     8881 acggcgaggt cagcgaatcg acatcccagt tatggttaag ctgcggtgat ggcagtggtc
     8941 caatccggct ggtgggcagc cgcagtacca cgttggcgct ttgagcgtct tctaatggtg
     9001 tgcccgctcc aatccgttcc tgaataacct gaggatccag acagaagggg tggggtaata
     9061 tctgcgtccg cttggggacc tgcccgcgaa tgcgttccgg tgccggtcct tctgaagact
     9121 cagcccaaaa aagtatgcct cccggatctg tggcccggcg tggcggctgc caatgtgcgt
     9181 ggagaaccca catatgttct tatatctacc ctgattattg ataaaacttc actgccccgc
     9241 gggcaagcga tccatattta ttccatcggc tgatggcgcg aaccagattt tagagcccat
     9301 ctccagcccc gctgttgaag tggaccctgc tgccgcccca acttgcccct gttttttagg
     9361 ttacattgtt aagaaaacgc gctataatta cgccatgatt atatcctgat tctgtcaagt
     9421 cgtggagagt aaaataatcg gtctatcctg ctcttttttc tctccagctt tgcttgacac
     9481 aatgatagcc ctgtgctaaa gttatttaca aatcaggaaa taaattccct ttgaccggga
     9541 aggagcgcaa ccttgaccaa gtcacgaacc caaatgatgg tagagcgttt acgggagctt
     9601 caggcatctt cgccagacat tgaagcctca gcaattgtga gcgttgatgg tttgaccatc
     9661 gcttcggcac tgcctcaggg cgttgaggaa gatcgcgttt ctgccatgtc ggctgccatg
     9721 ctctcccttg gcgaaagaat cgctggagag ttgggccgtg gctcattgga acaggtctat
     9781 atcaaaggcc aaaaaggcta tgtcgtcttg atgtcggtcg gacaagaagc tgtgcttact
     9841 gccctggcgc gtgaacaggc caagctgggc ctgattttcc tggatatgcg ccgcgcagcc
     9901 gaagacctga ctaaatatat ctaataaggc gaggacacat ggaaccaatc ccaaagagcg
     9961 gcttgttcta ccccaataaa atttcccgca ttgcactttt agcgctggaa gatgtgatgg
    10021 gcaaaaacgg cgtcaatgcc attcttaacc tcgccagtct gccccatttg attggcaatt
    10081 atccccccac cgatctgaat cgcgaatttg acttcgctga tttctcagcc atcaatggcg
    10141 ccctcgaaga gatgtatggc ccgcggggtg gccgtggtct ggcgttgcgc gccggccgcg
    10201 ccactttcga tgaaggcctg cgtaactttg gcgccctggc tggtgtgggc gatctggcct
    10261 tcaaagttct tcccctacaa gctaaactgc gcattggcct gcccgccatg gctcgcattt
    10321 tctcgcaaac cagcgatcaa ctcagcactg ttgaagaaaa agacgaccac ttcgtctaca
    10381 ccattcatcg ctgcccggtt tgctatggtc gtcataccga caaagcatct tgctttatcg
    10441 cggtcgggtt gctgcaatcg ggtctcaagt gggtttctgg cggcaacgaa tttcgggtga
    10501 atgaatccaa gtgcatggct gtcggcgatt cggtgtgcga gttcatcgtc tataaagaac
    10561 ccatttctta ggagcactcc ggatgacaag cgaaaagaaa gagactctgc tgattgtaga
    10621 ggatgatctc gatatcgcgg atatgttaaa cgcgtatttt cgcgttcagg gttatgaggt
    10681 catcacggtg aactggggcg aagacggcgt cagcacctgc cagaacaccc cgcccgatct
    10741 ggttattctg gacatccgcc tgcccgatat tgacggtttt gaagttgccc gtcaactgcg
    10801 cagcaaccgc cgcacccaga gcatcccgat catcttcctg accgagaaac gtgaccgcgt
    10861 ggatcgcctg accggtcttg aactgcgcgc ggaagactac atcaccaaac cgtttgacat
    10921 caccgaactg cgcttgcgcg ttcgcaatgc tttgcgccgg gctcagcagg gctcacttac
    10981 caacccggtc accggcatgc cagatggcag cctggtagac gagcacttga tgcagttcct
    11041 ccaatcgcca gaccgcgctc tgctcctggt ttcgcttgcc aacctcgacc acttccgcga
    11101 agtttatggc tttgttgcct cagatgatct gctccgtgcc gtcagcctga tgatgcagga
    11161 cactgtccgc gaactttgcg cccccgatga cttcctcggt cacatggata cgtccacctt
    11221 cctcatctac acccgtccgg caaatctccc ggcgctcaaa gaacgcatcc gtataaaact
    11281 ccaacaaagt tttgagtatt tctattcgac tcgcgaccgc gaaggcggca ccttcgaggg
    11341 cgagaaaggt ctttctgttc agcttcatga actgacccgc aaatcccagg tattccgcga
    11401 tctggcagag ttgaaagaaa ccctccaaaa tctttgccgt tcgtagaggc ccattgaagc
    11461 tcacatttgt tattccgacc tacaatgagg cggagaactt gccccggctg gtctccgcct
    11521 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natcctggaa ctggtacaaa
    11641 cccttgccaa ttgtgacatg gcagttggct cgcgctatgt ccccggcggc agcctggatg
    11701 aacgctggcc cgcctggcgc cgcggcctgt cggcctttgg caactggtat gcccgcacca
    11761 ttctgggcct gcccatgcgg gatgtgactg gtggcttccg cctctggcga aacgaaaccc
    11821 tgcgtgccat gcctctggat cgtgtccgct caaatggcta tgtcttccag gttgaaatgg
    11881 cctacgtggc gcatcgtttg ggcttcacat ttcgtgaaat ccccatccat tttgccgacc
    11941 gccgctgggg caagtcgaaa atgtccttgc gcatccaggc cgaggctgct ctgcgagtct
    12001 gggcgctcct ggggcgttat cgcaatttga agccctaacg cacaatctga ttgaggatgc
    12061 ttcccgtccc gcgcgatgga atgaccaccc catcgcgttc cagtgtgcct acataagcct
    12121 gctcacccgg gataatctgc cagccgctta ataccagcgg gaatggtccc gcggcggcga
    12181 tccattcgcc gttgtatttt cgcaccaggt gaacatgcct cccggttgcc atgccgccct
    12241 cgcaggaagc gtgcccaatc cggtcatcct gtgccagcca ggtgcccacc ggaacccggt
    12301 cagccgttgc aacatgcaga taaaaaatca cccagccggt ttgttcatac ccatcgccat
    12361 ccaggtcaac catcaccgca ccgttatcag aacgcaccac cagccccggc gccgaagccg
    12421 tgatccactc cgctgaaggg gtgcagccct tcacttctgc gtggggcgca aaatccactg
    12481 cgcccaacgg actgaacaca ccccaggcca catgcggtcc acctgtaaaa tgccacagaa
    12541 cgcccggctc aaagggcagc tccagtagcg gtgcaattaa gccttccgga ataattggac
    12601 tggcggcttg gctccacgga tcgccagcga attgcgtata gtcaatagaa aaactggcct
    12661 gcccatacag gacctctcgc cacccggttt cattgaacca ctgcgcaaac aaaacctgca
    12721 gcgccattgt ccccgggttc aggtccgggt ttgccggaat gctgctgcc
//
LOCUS       DAOPVS010000051        12712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_5034_length_12712_cov_63.577941, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000051 DAOPVS010000000
VERSION     DAOPVS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12712
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_12712_cov_63.577941"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1171
                     /locus_tag="PKL11_05915"
     CDS             140..1171
                     /locus_tag="PKL11_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ13174.1"
                     /translation="MSKKLYVVLAVLLAFAFVLSACTPKPVETKIKVCQVTDTGGIDD
                     KSFNATAWAGVQEAIEVYGVDGKYLESQEVADYEKNLNLFLEEGCDLIIPVGFMLTDA
                     TKAAAEANPDAKFSIVDVSYDPVVPNILGQVFQTDEAAFLAGYLAAGVTKTGKIGTFG
                     GFPIPTVTIFMDGLARGVAYYNEVHGTAVEVIGWDINNPDAGLFSSSFDDQDKGRELG
                     VSLMDEGCDIIMPVAGPVGLGTAAAMKERGGGYVIGVDSDWFLTAPDYADITLTSVLK
                     NMNVTVLESVKMVIDGNFAGGTTVGTLANKGVGLAPYHNLDSMVSAELKAEIEQLTKD
                     IVDGKVKTR"
     gene            1356..1616
                     /locus_tag="PKL11_05920"
     CDS             1356..1616
                     /locus_tag="PKL11_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNZ13175.1"
                     /translation="MDIIKVKANSRTAAVAGAIAGVIREHKHAEVQAIGAGAVNQAVK
                     ALVLARSYLAEDGITVICIPEFVDVEIDSKVRTAIKLVVEPR"
     gene            complement(1814..2587)
                     /locus_tag="PKL11_05925"
     CDS             complement(1814..2587)
                     /locus_tag="PKL11_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNZ13176.1"
                     /translation="MPRKAIALIVLIIAAITSLACLSSSATPTEAPTSAPIVTLSSTA
                     TKAAAPATATLEPSATPEPPTATPRPTATQTATPQPLATVSSESINLRTGPGTIYGVV
                     SALTQGTSLILLERTADSQWFHVQVADTGVEGWVSALVVELSVDPETLPLAAYIPPTP
                     SYIPPTKAPVPPTAAAEPTAVPTYDTHITIINSLSVSVTITLDGPIRTTVTVSAGSSI
                     VVDLPAGGYYFYATAPGFAPLEGNKTWDPGEWEWEFYAE"
     gene            2863..3804
                     /locus_tag="PKL11_05930"
     CDS             2863..3804
                     /locus_tag="PKL11_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ13177.1"
                     /translation="MNKYIPDYHWSNIFAMLRNLKAEPRICTAPLNHHLVVITGATSG
                     IGFAAARKYASRGADLLFVNRNEEKSIGLCEELKDAYNTNCDYLLADFSKLADIHFAA
                     EKLSKLDRKIDSLIHNAGVYNTRRLVTADHLEAVFQTNYLSTFILNYSLKEKFRNQAS
                     GRILFVNSEAYRFAAFGLNLDDLLWQKRRYSGLMSYGSAKLAQLLSMLKFNDYYTGSG
                     VTINAMHPGNVKTNSGQNNGIIYKLFKRLILDRNTQPADFAAEALYYLGVSDEVATRS
                     GKFFNLTTEEEPAPPALDWAAAEELWQISLKLGGLHA"
     gene            3797..5410
                     /locus_tag="PKL11_05935"
     CDS             3797..5410
                     /locus_tag="PKL11_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ13178.1"
                     /translation="MHKTNEQIVIVGAGMAGLTAAAYLSKAGYKVLLLEKNTRSGGLL
                     GTFSSNGFHFDTGPRALINSGIVKPILKELGVEWTCYENKISIGLEDRLFQVSSIEAL
                     EEYQRILIDLYPDDTEDIRRILAAMRELSEYTRVIYEFDNPYFGDQFSNKEYVIKRLL
                     PWTLKFLQALRKFNQFKLPMEDYLHQLTTRQPLIDILTQHFFRGTPTYFALGYFYVYL
                     DYFYPKGGTGALKDLLTQKVSEWGGQIELNKKVIQVRPAESKVIDEAGQEYRYDHLIW
                     AADLKTFYQSIDPVGLDATVTRRLDAERQRILASKGAESVFIMNIGVNRPPSYFKERG
                     GEHLFFTPSRQGLGETNGAQRVRLIQNFDQFSKSEVIAWLESYCDLTTYEISIPALRD
                     SSLAPEGQTGLMVSCLFDYQVIKNVENAGWYDEFKERVENRIISNLSRTIFSDLDQDI
                     LFKFSATPLTINKVSGSSEGAITGWSFETEPPVIYKLTDVGKSVITALPNVYKAGQWA
                     YAPAGVPIAMLTAWHAYQKIIKQSKRKRP"
     gene            5407..5823
                     /locus_tag="PKL11_05940"
     CDS             5407..5823
                     /locus_tag="PKL11_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13179.1"
                     /translation="MIIKIVLLIFILLEFSNVVVLYFFPGSKMANGVGVFSAWEKSKQ
                     YPEIHDFVRYLVYWVAGAKLIFLLLLGLIVLFADLNFQRLSLLALGFATVTFFWRMFP
                     LVRKMDRAGQLQPRYSLVLGGMILGLVVLFFAAALI"
     gene            6024..6845
                     /locus_tag="PKL11_05945"
     CDS             6024..6845
                     /locus_tag="PKL11_05945"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13180.1"
                     /translation="MSTVEHDHLPDANRLSIVAALVLLTYAATPFLRAPGQLISLGLF
                     GIRFDLPLNYGTALSTLAAVLAGAGASWLISAHPHLQRAMRLRHVFIPALTAWVIGVP
                     LSTLKVGAPWWGVLALGGVLLVLVLVAEYIAVEPDDVRHAPAAVGLTAVSFALYLILA
                     IALRAAQFRLYMLVPALTLALFAVAVRSLYLHLGGVWRVAWAVAIALFVGQLALGLFY
                     TGIPPLRFGLILLGPAYALTRLAGALSEGRPLRSALGEPAAMLAAFWLLAIFFNQ"
     gene            6853..7287
                     /locus_tag="PKL11_05950"
     CDS             6853..7287
                     /locus_tag="PKL11_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNZ13181.1"
                     /translation="MALSIGSGERAIMQQHVLRCLPTEACGLLGGLGQTVTAAIPITN
                     RLHSPTRFVMEPLEMLRAFRWLEEHELKLLGIYHSHPSGPAHPSPTDLTELASWEAAI
                     LIWSPAGPGWQARAFYLRPEGYDPVPIHWIDEAPQPPAETGV"
     gene            7340..7999
                     /locus_tag="PKL11_05955"
     CDS             7340..7999
                     /locus_tag="PKL11_05955"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ13182.1"
                     /translation="MILKFLAIAALSYLIGSIPFGWLVVMIARGRDVRTLGSGRVGGT
                     NVMRAAGCLAGVLTALLDAFKGVMALWAANWLLPGNPWAQALGPLMAVVGQVLSVFLV
                     ERQTDGKLLFRGGAGGATTFGGLVALWPPALIVVWPLAGLVFLLGGYASLTTIAVALS
                     GLLALIYQAITGAGPWAHVLYGVGTLILVLWALRSNLQRVRAGTERTVGLRAWRQKQG
                     K"
     gene            complement(8085..8939)
                     /locus_tag="PKL11_05960"
     CDS             complement(8085..8939)
                     /locus_tag="PKL11_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ13183.1"
                     /translation="MQRERISENVYWFQSEVYAQVTAGAIIGPQWAVLIDTLAMPEET
                     LAMRSFIEEQLGVPVRYIILTHSHADHAWGSCFFPGATVIASTECRRLMQETGAEALE
                     KARHQNPAFRQVKLVHPHLTFDEGTFYLRVGKKSLSLFATPGHSTDGISVLVEEDRVL
                     FAGDAFMPLPYIVGGNLDELTASIKKIGKLGLENIIQGHGDIILRGEIEEAIKSNLSY
                     LAAIRKAVRTAARRRNAVEALGQIEIESCGKSRVALSGLAEELHRRNVRALYRQIRDE
                     EEDPSTPI"
     gene            complement(9081..9431)
                     /gene="rplT"
                     /locus_tag="PKL11_05965"
     CDS             complement(9081..9431)
                     /gene="rplT"
                     /locus_tag="PKL11_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNZ13184.1"
                     /translation="MSRVKRGATAHLRHKKVLKLNQGYFGSRHRLFRRANEAMLKSMW
                     YATRDRKVRKRDLRKLWIVRINAAARLNGLSYSQLMHALKVADITLNRKVLADLAVRD
                     PNTFAAIVTRAKAA"
     gene            complement(9534..9764)
                     /locus_tag="PKL11_05970"
     CDS             complement(9534..9764)
                     /locus_tag="PKL11_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNZ13185.1"
                     /translation="MPRKLKTGKLKLKTHKATSKRFRLTGTGTVVRTKGGKSHLRRRT
                     AKRTKVLLSEMIVVEGKSIVKRVRRLAPNMEQ"
     gene            complement(9874..10959)
                     /locus_tag="PKL11_05975"
     CDS             complement(9874..10959)
                     /locus_tag="PKL11_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ13186.1"
                     /translation="MEAPRVGYFVSSHGFGHAARASAVMLALREQAPGVEFDIFTETP
                     EWFFKEALGGGYQYHLAQTDVGVVQSGPLDEDLPATVKRLAGMWPLRPERVDSLAYAL
                     QRTDCRLVICDISPLGIAAAYAAGLPVVLIENFTWDWIYAGYLKTEPRLEAFLPLLQI
                     AFAAADLHLQAEPYCQPAAGAQMVAPISRPPRTPRAQLRASLQIAAEAPVALVTMGGI
                     PAQFDFLSALQAHPEITFIIPGGSAQVEQRGNLRLLPHHSPFYHPDLMQACDVVVAKA
                     GYSTIAEAWYAGVPFVWVSRPRFRESGVMSAYLRQSFDGFELSEAEFAGGGWAARLPA
                     LLEKPRRARPGVNGAHQAARLILDTFI"
     gene            complement(11039..11326)
                     /locus_tag="PKL11_05980"
     CDS             complement(11039..11326)
                     /locus_tag="PKL11_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13187.1"
                     /translation="MKKAKIAILIALLALTVLATGAAAMASANYRLDWFAPATGTGGS
                     AESASYAMSFTVGQSAIYPAASTSYRAGLGFWYGWGEIAEFVQVFLPAILR"
     gene            complement(11337..12455)
                     /locus_tag="PKL11_05985"
     CDS             complement(11337..12455)
                     /locus_tag="PKL11_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13188.1"
                     /translation="MNKKLSFILLALVLSLGIAALNLGAGAAQDTGPNGEPETITPGA
                     IPIQGRLTDAAGNPLDGTYTVVFSLYEVATGGTAICSQTRSISVVDGLFSDYLDNCYN
                     VLTGQKVWLGIKVGDDDEMTPRQVILAVPYALALVPGATIISDIDGVLEVESVGTPGD
                     YDAFLAYADGTGEAVSGMATGGSGVYGESLTGIGVLGYSETGTAIQATGTGVIASSAV
                     SYVWISGNNATKFYAADDIVINGNGMGGADIYRGSTAGTRYIMIPITVPGPIYGQDVT
                     VSALDIYWAGQTEFTSITSFRLRRQVGANNYVEIVFDDADYTCNVDPNGCIIHKDLTT
                     NNVLTADSGILYLVFGTMFNGDADWLRLGGVRLTLEHD"
BASE COUNT         2547 a         3657 c         3805 g         2703 t
ORIGIN      
        1 cccaatctgc acccaaaccc gtaattttca tccttgcatg gcggtgatat cggttgtata
       61 ctagtaacaa gtgcggagca aacttgtctg acatttgctc cggaaacccc ccattcaccc
      121 cttatgagga gaggaaagaa tgtctaagaa actgtatgtt gtgttggctg tgctgctggc
      181 cttcgccttt gtgctgagcg cctgcactcc caagccggtc gagaccaaaa tcaaggtctg
      241 ccaggtcacc gatacgggcg gtattgacga caagtcgttc aacgccaccg cctgggcggg
      301 cgtccaggaa gccatcgaag tttatggcgt ggacggcaaa tacctggaat ctcaggaagt
      361 tgcagactat gagaagaacc tcaacctctt cctggaagag ggctgcgacc tgatcatccc
      421 cgttggtttc atgctgaccg atgccaccaa ggctgcggcg gaagccaatc ccgatgccaa
      481 gttctccatc gtggatgttt cctacgatcc cgtggtcccg aacatcctcg ggcaggtctt
      541 ccagacagac gaagccgcct tcctggctgg ctacctggct gctggcgtga ccaagaccgg
      601 caaaatcggc accttcggcg gcttccccat ccccaccgtc accatcttca tggatggtct
      661 ggctcgcggc gtggcctact acaatgaagt tcacggcacc gctgtggaag tcatcggctg
      721 ggacatcaac aacccggatg ccggcctgtt ctccagctcc ttcgatgatc aggacaaggg
      781 ccgcgaactc ggcgtctccc tgatggacga aggctgcgac atcatcatgc ccgttgccgg
      841 tcctgtgggc ctgggcactg cggctgcgat gaaagaacgc ggcggcggct atgtcatcgg
      901 cgtggattcc gactggttcc tgaccgcccc cgactatgcc gacatcaccc tgacctctgt
      961 tctgaagaac atgaacgtga ccgtgctcga atcggtcaag atggtcattg atggcaactt
     1021 cgctggcggc accactgttg gtaccctggc gaacaaaggc gttggcctgg ccccctatca
     1081 caatctggac agcatggtct cggctgaact gaaggccgaa atcgaacagc tgaccaaaga
     1141 catcgttgat ggcaaggtca agacccgcta aaccgggtag tgtttgaacg cccttttgcc
     1201 cccgcccccc ggcgggggca tttttattca cgatacaagt tgtctaaacc gcttgtaatt
     1261 gtctgtagaa tcttgtacaa tcgcgataac gatttgaccc tccccctggc aacccattct
     1321 ccaaccatcc aactcactag agaaaggccg catccatgga catcatcaag gtcaaagcaa
     1381 attcccgcac ggccgctgtt gcgggcgcca tcgcaggggt aatccgcgag cataaacatg
     1441 ccgaagtgca ggccattggc gctggcgctg tcaatcaggc cgttaaggcg ctggtgctgg
     1501 cgcgcagcta cctggcagaa gacggcatta cggtgatttg cattcctgaa tttgttgatg
     1561 tagaaattga cagcaaggtt cgtaccgcca tcaagctggt ggtcgagccg cgctgagcag
     1621 tcctccgccc gtttgcacaa ctgagcttgc ccattggggt tcgttcaacg cggggcctgc
     1681 aaggctcggg caggggctga ccgcccggcg taacattaat attacaccgg gcgcacaacc
     1741 tattcaggtg gcaggcacac aggaccgccc ctacggcaca ataagccgcc ggagtttcac
     1801 gatcgcgcgg cagctattcc gcgtaaaact cccattccca ttcaccggga tcccaggttt
     1861 tgttgccttc cagcggggca aaacctggcg cagttgcgta gaagtagtac ccgccagcgg
     1921 gcagatcgac cacaatgctg ctgccggcag aaaccgtgac ggtggtgcgg atgggcccat
     1981 ccagcgtgat ggtgacgctg acgctgaggc tgttgatgat ggtgatatgc gtgtcatagg
     2041 tcggcacggc agtgggttcg gcggctgcag tgggcggcac gggggccttg gtcggcggga
     2101 tgtagctggg cgtgggcgga atataggccg ccagcggcag cgtctcgggg tcaaccgaca
     2161 gctcaaccac cagcgccgag acccaaccct ccacgccggt atcggccacc tgcacatgga
     2221 accactgcga atcggcagtg cgctccagca gaatcaagct ggtgccctgc gtcagcgcgc
     2281 tgaccacacc gtaaatggtg ccggggccgg tccgcaggtt gatgctctcg ctgctgacgg
     2341 tagccagcgg ctgcggggtg gcggtttgag tggcggtggg gcgcggggtg gccgtgggcg
     2401 gttcgggcgt ggccgagggc tccagcgtgg cagtggccgg cgcggcggcc tttgtagccg
     2461 tggatgaaag cgtgacaatt ggcgccgagg tgggcgcttc tgtcggcgtg gcgctggaag
     2521 acaggcacgc cagcgaggta atcgcggcga tgatcagcac aatcagggca atggctttgc
     2581 gaggcatgga tgttttcctt tgaactgtgg gggaactgga aattaagttg attgtagcac
     2641 gattcaatcc gcaccttgcg ccgtgcaggc ggagattttt atccttacaa ggtgctttac
     2701 gaggccgccc gactgcaaaa tctgcttttc cggctcaggt tgtgctgaag cggattaatt
     2761 tgatagccta tacaggtcat gtataatact tggaacagat cgggttaaag attaaaatat
     2821 agtttggcat cagcacagaa gcaacacctg gtcggaggaa gtttgaataa atacatcccg
     2881 gattatcact ggtccaacat ttttgccatg ctgcgcaatc taaaagcgga gccgcgcatt
     2941 tgcacggctc ccctcaacca ccatttggtg gtgatcacgg gagccacttc cggcatcggt
     3001 tttgcagccg cgagaaaata cgcctcccgc ggtgcagatc tcttatttgt gaatcggaat
     3061 gaggaaaaat ccatcgggct ttgtgaagaa ttgaaggacg cctacaacac caactgtgat
     3121 taccttctgg cggacttttc gaaactggcc gatattcatt ttgcggcaga aaaattatca
     3181 aagctggacc ggaaaattga cagcttaatt cacaatgccg gcgtttacaa cactcgccgg
     3241 ctggtcactg ctgaccacct tgaggctgtt tttcaaacca attatttgag cacgttcatt
     3301 ttgaattatt ccctgaaaga aaagtttcgg aaccaggcca gcgggcggat cctctttgtc
     3361 aattcagaag cctaccggtt tgcagcattt ggcctcaatt tggacgacct cttgtggcaa
     3421 aaaaggcgct attcgggcct catgagctat ggctcggcca agctggcgca gttgctttcg
     3481 atgctaaaat ttaacgatta ctataccggc agcggagtca ctatcaatgc catgcacccc
     3541 ggcaacgtga agaccaacag cgggcaaaac aacggcatta tttataaatt gttcaagcgg
     3601 ctgatcctgg atcgcaatac gcagccggca gattttgcgg ctgaggcgct ctattatctg
     3661 ggcgtttctg acgaagtagc cacgcgctcc ggcaagttct tcaacctgac gaccgaagaa
     3721 gaacccgccc cgccagcgct ggattgggca gcggcagaag aactgtggca gatcagttta
     3781 aaactaggtg gattacatgc ataaaacaaa tgagcagatc gtgattgtcg gcgcagggat
     3841 ggccgggtta accgccgcgg cctatctttc taaggccggg tacaaagtgc tgctgctgga
     3901 gaagaatacc agaagcggag gcttgctggg cacctttagc agtaatggct tccattttga
     3961 taccggtcca agagccttga tcaattcggg gattgtcaaa cccatcttga aagagctggg
     4021 ggtcgagtgg acgtgttatg aaaataaaat ctcaattggc ctcgaggacc ggcttttcca
     4081 ggtcagttcg attgaggccc tggaagaata ccagcggata ttgattgacc tttaccccga
     4141 tgacaccgaa gatatccggc ggattcttgc cgcaatgcga gaactatcgg aatacacccg
     4201 ggtgatctat gaatttgaca acccgtattt cggcgatcaa ttcagcaaca aagaatatgt
     4261 gatcaaacgg ctgctgccgt ggacgttgaa attcctgcag gcattaagaa aattcaatca
     4321 gttcaagctg cccatggaag attacctgca tcagctaacc acccggcagc cgctgattga
     4381 catcctgacc cagcacttct tcaggggcac gcccacgtat tttgccctgg gctattttta
     4441 tgtttacctg gattattttt acccgaaagg cggcaccggc gcgttgaagg acctgctgac
     4501 ccagaaagtg agcgaatggg gcgggcagat tgagctgaat aaaaaggtta tccaggtcag
     4561 acctgccgaa tcaaaggtca ttgatgaagc gggccaggaa taccgctacg accatttaat
     4621 ttgggcggca gatctaaaga ccttttacca gagcattgat ccggttggct tggatgccac
     4681 cgtgacgcgc cgtttggatg ctgaaaggca gcggattttg gcctccaaag gggctgaatc
     4741 ggtcttcatc atgaatatcg gcgtcaaccg cccgccatcc tacttcaagg agcgcggcgg
     4801 ggagcacctg ttcttcacgc cctcccggca ggggttgggc gagaccaacg gggcgcagcg
     4861 ggtgcgcctc atccaaaatt ttgatcagtt ttccaagagc gaagtgattg cgtggctgga
     4921 aagctactgc gacctgacga cctatgaaat ctccatccct gcactccggg atagttccct
     4981 ggcgcctgaa ggccaaaccg gcctgatggt tagctgcctc tttgactacc aggtgatcaa
     5041 aaacgtcgaa aatgctggct ggtacgatga atttaaggag cgggtggaaa atcggatcat
     5101 cagcaacctg tcccggacaa ttttcagcga tcttgaccag gatattcttt ttaagttctc
     5161 cgccacgccg ctgacgatta ataaagtctc cggcagttcc gaaggcgcca tcaccggatg
     5221 gtcttttgaa actgagccgc cggtcatcta taagctcacc gatgttggaa agtcggtcat
     5281 caccgcgctg cccaatgttt ataaagccgg gcagtgggcc tatgcgccag ccggtgtgcc
     5341 cattgccatg ttgaccgcct ggcatgccta tcaaaaaatc atcaagcagt caaaaagaaa
     5401 acgcccatga tcatcaagat tgtgctcctg atatttatcc tgctggaatt ttcaaacgtt
     5461 gtggtcttgt attttttccc ggggtccaaa atggccaatg gcgttggggt tttctcggcc
     5521 tgggaaaagt ccaaacaata ccccgaaatc cacgactttg tgcgctatct ggtctactgg
     5581 gtggcgggcg ccaagctgat ctttctcctc ctgctgggct tgattgttct gttcgccgac
     5641 ctgaattttc agcgcttaag cctgctggcg ctgggttttg ccacggttac tttcttctgg
     5701 cggatgtttc cgctggtgcg aaaaatggac cgcgccgggc agctccaacc ccgatactcg
     5761 ctggtgctgg gaggaatgat cctgggattg gtggttctgt ttttcgccgc tgcactgata
     5821 taaaaaaacg ggggccctag agcctggccc cgggttaacc cggcggtcat tgcaattgcc
     5881 gcagttccct gcgccagaac gggctgcgcc tcggtaatta atggcctgcg ttgcccttac
     5941 gctacaatta caggcatctt tgggttgcct tcttccgcat tttttgcatg gcatttcggc
     6001 gcgggtgagt gtacaatcag ggtatgtcca cggttgaaca tgatcacctg cccgacgcca
     6061 accggctgag cattgtggcc gcgctggtgc tgctgaccta cgccgccacg ccgtttttgc
     6121 gtgcgccggg gcagttgatc tcgctggggc tgtttggcat ccgctttgat ctgccgctca
     6181 attacggcac ggcgctttca acgctggcgg cggtgctggc tggcgcgggc gccagttggc
     6241 tgatcagcgc acacccgcat ttgcagcgcg ccatgcggct gcggcatgtc ttcatcccgg
     6301 cgctcaccgc ctgggtgatc ggcgtgccgc tgtccacgtt gaaggtcggc gcgccgtggt
     6361 ggggggtgct ggcgctgggc ggcgtgctgc tggtgctggt gctggtggct gaatatatcg
     6421 ccgttgagcc ggacgatgtg cgccatgcgc ctgcggctgt gggcctgacg gcggtctcgt
     6481 ttgcgctgta cctgatcctg gcgattgccc tgcgggcggc gcagttccgg ctttatatgc
     6541 tggtgcccgc gctgacgctg gccttgtttg ccgtggcggt gcgctcgctt tacctgcacc
     6601 tggggggcgt ctggcgggtt gcctgggcgg tggccattgc cctctttgtg gggcagttgg
     6661 cgctgggtct gttttatacc ggcataccgc cgctgcgctt tgggctgatt ctgcttggcc
     6721 cggcctatgc cctcacccgt ctggcgggcg cattgtctga gggccgcccg ctgcggtcag
     6781 ccctgggcga gcccgctgcc atgctggcgg ccttctggct gctggcaatt ttcttcaatc
     6841 agtgagttgg atatggccct gtccattggc tcaggtgaac gcgccatcat gcagcagcat
     6901 gtgctgcgct gcctgcccac cgaagcctgc gggctgctgg gcgggctggg tcagaccgtg
     6961 accgcggcca tccccatcac caaccggctg cacagcccca cccgctttgt gatggagccg
     7021 ctggaaatgc tgcgggcctt ccgctggctg gaagaacacg agctgaagct gctgggcatc
     7081 taccactcgc accccagcgg cccggcgcac ccctcgccca ccgacctcac cgagctggcc
     7141 tcctgggagg ccgccatcct catctggtcg ccggctggcc ccggctggca ggcccgggcg
     7201 ttttatctcc gcccggaggg ttacgacccg gtgccgatcc actggattga cgaagcgccg
     7261 caacctcccg ccgaaaccgg ggtttaatgt taataacgca ggggaacctg atactttact
     7321 gatatgggtg actgaattga tgatcttgaa atttctcgcc attgcggcac tcagttattt
     7381 gattggctcc attccgtttg gctggctggt ggtgatgatt gcccgcgggc gcgatgtgcg
     7441 caccctgggc agcggccgcg tgggcggcac caatgtaatg cgggcggcgg gctgcctggc
     7501 cggtgtgctg acggccctgc tggatgcctt caaaggggtg atggcgctct gggcggccaa
     7561 ctggctgctg cccggcaacc cctgggcgca ggccctgggc ccgctgatgg cggtggtggg
     7621 gcaggtttta tccgtctttc tggtggaacg gcagaccgat ggcaagctgc tgtttcgcgg
     7681 cggcgcgggc ggggccacca cctttggcgg gctggtggcg ctctggccgc cggcgttgat
     7741 cgtcgtctgg ccgctggcgg ggctggtgtt cctcttgggg gggtacgcct cgctgaccac
     7801 cattgccgtg gccctcagcg gcctgctggc gctgatctat caggccatca ccggggccgg
     7861 gccctgggcg catgtgctgt acggcgtggg cacgctgatc ctggtgctgt gggcgctgcg
     7921 ttccaacctg cagcgggtgc gcgccggcac cgagcgcacc gttggcctgc gcgcctggcg
     7981 gcagaagcag ggcaaataga gggattgggg attagtgtca ggcaatccat aaaaaaacga
     8041 gcctctaaaa ggctcgttaa ttctaaaaac cgaaaaccca aatcctagat cggtgttgag
     8101 gggtcttcct cttcatcccg gatctggcgg taaagcgccc gcacattgcg gcggtgcagc
     8161 tcttcggcca ggccgctgag cgccacgcgg ctcttgccgc aggactcaat ttcgatttgg
     8221 cccagggctt ccaccgcgtt gcgtcggcgg gcagccgtgc gcaccgcctt gcggatggct
     8281 gccaggtagc tcaggttgga tttgatggcc tcttcaatct cgccgcgcag gatgatatca
     8341 ccatgcccct ggatgatgtt ctccagcccc agcttgccaa tcttcttgat gctggcggtc
     8401 agttcgtcaa ggttgccgcc cacaatgtag ggcagcggca taaaggcatc accggcaaac
     8461 agcacccggt cttcttccac cagcaccgaa ataccatcag tgctgtggcc aggggtggca
     8521 aacaggctca ggctcttctt acctacgcgc aggtagaagg tgccttcatc aaaagtcagg
     8581 tgcggatgca ccagcttgac ctgccggaaa gccgggtttt gatgccgggc cttctccagc
     8641 gcctcggcgc cggtttcctg catcaggcgg cggcactcgg tgctggcaat caccgtggcg
     8701 ccggggaaga agcagctccc ccaggcatgg tcggcgtgtg agtgggtgag gatgatgtag
     8761 cgcaccggca cgcccaactg ctcctcaatg aagctgcgca tggccagcgt ttcttcgggc
     8821 atggccagcg tatcaatcaa cacagcccac tgcgggccga tgatggcgcc agccgtgacc
     8881 tgagcataaa cctcactctg gaaccagtag acattttcag aaatccgttc gcgctgcata
     8941 aaaccatcca acagttgaaa taattacaat ggcggcgaaa tttcgccgtc aagacaatag
     9001 aatagcataa aagagactaa aagtcaaaaa aatgcccctc tccacaggga gaggggcaaa
     9061 aagagtacaa aaatgggctg ctaggcagct ttggcgcggg tcacaatggc cgcaaaggta
     9121 ttgggatcgc gcacggccag gtcggccagc actttgcgat tcaaggtgat gtcggccact
     9181 ttgagggcgt gcatcaactg gctgtagctc agtccgttca ggcgggcggc cgcattgatg
     9241 cggacgatcc acagcttgcg caggtcgcgc ttgcgaacct tgcggtcgcg cgtggcgtac
     9301 cacatgcttt tgagcatggc ttcgttggcg cggcggaaca ggcgatggcg agagccaaaa
     9361 tagccctgat tcagcttcaa tactttcttg tggcgcaggt gggcggttgc gcctctcttc
     9421 acacgagaca tttactttac tcctttgcga accccggggc gccggtgtgc atcacccgtt
     9481 gtatgcaccc tgcggtcgca caaatcaaaa acgaatcctg atgggcgtta agcctactgc
     9541 tccatgttgg gggccagtcg gcggacgcgc ttgacaatgc tcttgccttc gaccacgatc
     9601 atctcagaca gcaagacctt ggtgcgcttg gcagtgcggc ggcgcagatg gcttttgccg
     9661 cccttggtgc gaaccacggt accggttccg gtcaggcgga accgcttgga ggtggcctta
     9721 tgggtcttga gcttcagttt gccggttttt agcttccgag gcacagcgat tttcctctct
     9781 ttcaaagata atagatccac gcgcctgcga aggcgcaagg ggctattata ctataagttc
     9841 cgattctgtg caggcgagtt ttgcgtatcc ccgctatata aatgtatcca ggatcaggcg
     9901 ggcagcctga tgcgccccgt tgacgccggg ccgcgcccgg cgcggttttt ccagcagggc
     9961 tggcagccgg gccgcccagc cgccgccggc aaattcggct tcgctcagct caaagccgtc
    10021 aaatgactgg cgcaaatagg ccgacatcac gcccgattcg cggaagcgcg gccgcgagac
    10081 ccagacaaac ggcacgcccg cataccaggc ttcggcaatg gtgctgtacc cggctttggc
    10141 caccaccacg tcgcaggcct gcatcaggtc ggggtggtag aagggcgaat ggtgcggcag
    10201 caggcgcagg ttgccccgct gctccacctg ggcgctgccg ccggggatga tgaatgtgat
    10261 ctccgggtgg gcctgcaggg cgctcagaaa atcaaactgg gccggaatgc cccccatcgt
    10321 caccagcgcc acgggggcct cggcggcaat ctgcaggctg gcgcgcagtt gggcgcgcgg
    10381 ggtgcgcggc gggcggctga tgggcgccac catctgggcg ccggcggcgg gctggcagta
    10441 gggttcggcc tgcaggtgca ggtcggcggc ggcaaaggca atttgcagca gcggcaggaa
    10501 agcctccagc cggggttcgg ttttcaggta gcccgcataa atccaatccc aggtgaagtt
    10561 ctcaatcaaa accaccggca gcccggcggc ataggctgcg gcaatgccca ggggcgaaat
    10621 gtcgcagatc accaggcggc agtccgtgcg ctgcagggca taagccagcg aatcgacccg
    10681 ctcggggcgc aggggccaca tgccagccag acgttttacc gtggcgggca ggtcttcatc
    10741 cagcgggccc gactgcacca cccccacatc ggtctgcgcc agatgatact gatagccacc
    10801 gcccagcgcc tctttgaaaa accattcggg cgtctcggtg aaaatgtcaa actcaacccc
    10861 cggtgcctgt tcgcgcagcg ccagcatcac cgcgctggcg cgggccgcgt gcccaaaccc
    10921 atgcgatgag acgaaatagc caacccgggg cgcttccatg ggcgcaattg taccatgccg
    10981 ggggcgggct gcaacctctc cggcatggca aacaattgga ctgtattttt acgggtgctc
    11041 agcgcaggat ggctggcagg aacacctgca caaattcagc gatctcgccc cagccatacc
    11101 agaagcccag cccggcccgg tagctggtgc tggccgccgg atagatggcc gactgcccca
    11161 ccgtgaagct catggcatag ctggccgatt cggccgaccc gccggtgccg gtggccgggg
    11221 cgaaccagtc cagccggtaa ttggccgagg ccatggcggc ggcgccagtg gccagcaccg
    11281 tcaacgccag aagcgcaatc aagatggcaa ttttagcttt tttcatggcg ccctcgctaa
    11341 tcatgttcca gcgtcaggcg cacaccgccc agccgcagcc agtctgcatc tccgttgaac
    11401 attgtgccga acaccaggta gaggatgccg gaatcggcgg tcaggacgtt gttggtggtc
    11461 agatctttgt ggatgatgca gccgttgggg tctacattac aggtgtaatc cgcgtcatca
    11521 aagacaattt cgacatagtt gttggctccc acctgccggc gcagccgaaa actggtaatg
    11581 ctggtgaatt cggtttgccc ggcccagtag atatccagcg cggagacggt tacatcctgt
    11641 ccgtagattg gaccggggac ggtgatcggg atcatgatgt atctggtgcc cgccgttgaa
    11701 ccccgataaa tatctgcgcc gcccatgccg ttcccgttga tcacaatgtc gtcagcggca
    11761 tagaatttcg tggcattgtt gccgctgatc cacacatacg aaaccgccga gctggcgatg
    11821 acgcccgtcc cggtggcctg gatggcggtg ccggtctcgc tgtatcccaa aaccccaatc
    11881 ccggtcaggc tttcaccata gacgccagac ccgccggtag ccatgcccga aaccgcctcg
    11941 ccggtcccat cggcataggc cagaaaggca tcgtaatcgc ccggtgtgcc cacgctttca
    12001 acctcgagaa cgccgtcaat atcgctgatg atggtggccc ctggcaccag ggccagcgca
    12061 tagggcaccg ccagaatgac ctgacgcggg gtcatttcgt catcgtcgcc caccttgatg
    12121 cccagccaga ccttttgccc ggtcaggaca ttgtagcagt tatccaggta gtcgctgaac
    12181 agcccatcca ccacggagat ggagcgggtc tgcgagcaga tcgccgttcc accggtggca
    12241 acttcataca ggctgaacac aaccgtatag gtgccatcca gcggattgcc ggcggcatcg
    12301 gtcaggcggc cttgaatcgg gattgcgccg ggggtaatgg tctcgggttc gccattgggg
    12361 ccggtgtcct gggcggcgcc ggcgcctaaa ttgagcgcgg caatgcccag gctgagtacc
    12421 agcgccagca ggataaacga caatttcttg ttcatgatcc aatactccat tctcaattca
    12481 aatcaattta tggctgttgg cccacaatgg gcagatacag gcagcatgac caggggcatc
    12541 ctccttctga ccggcgcgcg gcggcgcagt caaaacatct ggcaggtcgg cgtgaacctg
    12601 cccgggtgag gtaacttccg ataaagccgt ttgccagatt ggcgcatgta ccgctcaggc
    12661 agtatggctc tgcgttgagc gctttttaga aatgccaata aggtgatgat gc
//
LOCUS       DAOPVS010000052        11594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_5670_length_11594_cov_56.274981, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000052 DAOPVS010000000
VERSION     DAOPVS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5670_length_11594_cov_56.274981"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..742
                     /locus_tag="PKL11_05990"
     CDS             341..742
                     /locus_tag="PKL11_05990"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNZ13189.1"
                     /translation="MDPVNANCRTIDEYIARFPDDIQTILVELRAVIRAAAPEAEEKI
                     SYQMPTFALNGNLVHFAVHKNHIGFYPAPSGIEAFKSELSGYACSKGAVRFPIDRPLP
                     LDLIAKIVRFRVAENMKKAAQKPRQKAGHQR"
     gene            739..1416
                     /locus_tag="PKL11_05995"
     CDS             739..1416
                     /locus_tag="PKL11_05995"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNZ13190.1"
                     /translation="MSKLILSALRTFLIGALVLGVLLFLPAQTLNYWQAWVFIIVFQT
                     AVNAIGLYLALKDPALFERRKKVGPAAEQNPAQKIIMSLALLSLVALLVVCALDHRLG
                     WSRMPVVASLIGDLLVMLGLYLNFIVFKANSFGASTIQVVEAQKVISTGPYALIRHPM
                     YGGVLIMVAGVPLALGSWWGLLILALVLPGLVWRILDEEQTLKRDLPGYLEYMQKVRY
                     RLVPYLW"
     gene            1437..2210
                     /locus_tag="PKL11_06000"
     CDS             1437..2210
                     /locus_tag="PKL11_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNZ13191.1"
                     /translation="MTASQTTQPPLTQADIEAGLRRLGLERGQIVEVHSSLSRFGDVA
                     GGPAAVVDALMSVIGPQGAIVMSAYPVSKPLPLSEAEKSWGVLAKVQTYPESYRGPTG
                     MGVIADEFCRRPGTILGPGWHRVCAWGRDAQKLSQGYQALLALDGWALLLGVDIERLS
                     SMHQAEKEPLPPEITQYVAPPTEIRQRYPADYYIAYGQTPENAWLKIQARAESEQIIK
                     IGQIGQATCRFFKARPVVEMYAEALRTDPWGLYGVKKSA"
     gene            2324..3373
                     /gene="amrS"
                     /locus_tag="PKL11_06005"
     CDS             2324..3373
                     /gene="amrS"
                     /locus_tag="PKL11_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNZ13192.1"
                     /translation="MLLDRLDTLTAPAWLSEPAEGGAVRCLACAHRCLIQPGRRGVCQ
                     VRFNRAGELRVPWGYVAALQDDPIEKKPFAHLLPGARALTFGMLGCDFHCDFCQNWLT
                     SQTGRDPAAERGALYIEPVTPEQVVRVAQRVGAQVVVSSYNEPLITTEWAVDIFKLVR
                     AAGLRCAYVSNGHATPQALAALRPYLSAYKVDLKTMQAEQYRALGGRLEAVLATIQAA
                     HDLGLWVEVVTLVIPDYNDSPTELWEAARFIASVSRDIPWHVTAFHPDYQMTDRPPTG
                     SEALQRAAEIGQEAGLNYVYAGNLPGRVGSLEDTFCPHCQARLIERRGYTIRANRITS
                     AGACPNCGAAIAGVW"
     gene            complement(3483..4934)
                     /locus_tag="PKL11_06010"
     CDS             complement(3483..4934)
                     /locus_tag="PKL11_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ13193.1"
                     /translation="MSDFPLLTLAIIVFIVLMFFAALYMASRYKRCPSDKILVISGRV
                     GKGMSSRCMHGGGAFIWPVAQEHRYMSLTPLTINIPLQNALSLQNIRINVPSTFTVGI
                     STQPEVMTVAAERLVGLTPALIEAMAKEIIFGQLRLTVASLTIEQINQDRESFLEAVR
                     KNVEPELNKIGLYLINVNITDITDESDYIESIGKKAASEAVNQAKVDVAQQTKKGSIG
                     ESDAKREETIRVSENLAEAEKGKTAAMAGQRIFVQQREAEAVRGENNSRAEIAASNAE
                     LEVQKATAMQRAEIAKREAEKAIQIAEYEARMALLKAEEIAQQEINKTKQEIDAEANA
                     ERVRREARGEADSILMRYQAEAEGLRTLLEAKAVGYRGLIESCNGDAQAAASLLVIEK
                     LSDMVQAQVEAISRMRIDKITVWDGGSHNGSGNAAADFVSGFVRAIPPLQEIAAMAGI
                     ELPAYLGRAMPQTPEAAPQPPEAEKPTPPADKK"
     gene            complement(4955..5533)
                     /locus_tag="PKL11_06015"
     CDS             complement(4955..5533)
                     /locus_tag="PKL11_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13194.1"
                     /translation="MGLNTIYLICAIGGGTVLLLRLVMMIIGLDHGDTPDSGVDLDHD
                     GSVDAPDGNIGMKLLSLQSIAGFFTMFGLVGLGLLQVKAAPIWSLLGALAAGLVTAWV
                     TAMIFYQMRRLQSEGTMVIQDAIGQIGSVYLTIPEQGTGVVSVAVKGTLRSLDAMSEA
                     GKRIPTGAMVRVTGVTAGKILVVVEQQPPENS"
     gene            complement(5670..7274)
                     /locus_tag="PKL11_06020"
     CDS             complement(5670..7274)
                     /locus_tag="PKL11_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNZ13195.1"
                     /translation="MTDLELSHQMDELLQALYPADQPGAAVIVMQAGQPLLRKGYGLA
                     HLELGVPVDPAMIFRIGSVTKQFTAVCILMLLEQGKLSLQDDLTRYLPDYPTGGRTIS
                     IEHLLTHTSGIKSYTSLPEWYPLWRKDLTLDELIALFKDQPFDFEPGEKFQYNNSGYI
                     LLGAIIEKISGMPYAQFLQTQIFDRLGLTHTLYDDTARLVPGRVAGYSKGLDGLINTP
                     YLSMTHPYAAGALISNVDDLARWNAALTAGELLRPETLALAFQPYHLNNGESTGYGYG
                     WSIWDYAGDTFIEHGGGINGFACGAVRVPAAGVYVALLTNQDDSINDPNLVAFKLAAM
                     AIGKPYADPTPIDLPAEVLADYVGVYAVNDKAERVVTCEDGQLYSQRTGGPRLRLYPY
                     AEDAFFIKESAGDQFCFERTADGRVSGMQITRRLGPVERAPKTDRPLPQPRTAIELDP
                     AALGRYVGAFELAPGMTLMVTLTDGQLYLQAPGQEKIALFAETPERLFPRVEALTLDY
                     SFDAQGQATGFVLTQGPETYPCRKVG"
     gene            7429..7950
                     /locus_tag="PKL11_06025"
     CDS             7429..7950
                     /locus_tag="PKL11_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13196.1"
                     /translation="MSGYSPNFNLPAYGKYKQSSSKSYLIKLIIIAVVAAGLLAIMDV
                     LLGVFVGVVIIAGGLFQMSKSSRGWDGAIQAKDQMAFSDTQPDGTINTYTKYELKVER
                     APGKVETLKWTDNSGAFEYYRVGEQVRHHPGCDFPEKFDKSNDEQVICVACGELYDLP
                     TETCPKCQVPLLK"
     gene            7981..8784
                     /locus_tag="PKL11_06030"
     CDS             7981..8784
                     /locus_tag="PKL11_06030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13197.1"
                     /translation="MDDLSDIAEYYNTDPQHEADRLDRHQLELDLTWRYLERHLPSAA
                     KILEIGAATGRYTLELARRGYTVTAVDLSAGLVEHCAARLAEAGLAARVRLVVADARD
                     LSAITETDYDAVLLMGPLYHLMLEADRLLVLQQVYARLRPGGLIFSALISRYGILGDL
                     IRNIPDWIDDPEVASFMARGRRPDDYPPGGFRGYFARVEEIIPLHAAAGFETLTLAGV
                     EPAISADDESYNRLVGPQRQKWLDLLEAVSAEPTLVGASRHLLYIGRRP"
     assembly_gap    8833..8932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8933..9487
                     /locus_tag="PKL11_06035"
     CDS             <8933..9487
                     /locus_tag="PKL11_06035"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNZ13198.1"
                     /translation="AMTPEIIIGIVGSAALLFISRAALRTPGAHGFYRFFAWEGLLIL
                     FLLNARRWFVQPLALHQIISWGLLLISLGLVLAGLSLLRRRGQPDPQRADEALLPLEK
                     TTALVTTGIYRYIRHPLYSSLLFLGWGIFFKQPAGWGAALALGVSVFLVLTARAEERE
                     NLRYFGAAYQAYRQRTKMFVPFIF"
     gene            9504..10142
                     /locus_tag="PKL11_06040"
     CDS             9504..10142
                     /locus_tag="PKL11_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13199.1"
                     /translation="MKKLTLILLLALTLLLAACGGGKDQRPHSETVAEDKVVDLACGA
                     LSRKDCLARLCADPAACPVLIALAEPAVFDFIATYAACPDCNTPDFAPEDGIGKCVEY
                     QTSDAPSGWEVTFWVSENCTFRYGSPTESRVVVRINPQTLEIKKISPSVEIIADPLYC
                     QNKADCVSLSGSGVPFTGCSNSFYGPLNWSGYYAGTECTCQKHQCVAAAPKK"
     gene            complement(10197..10664)
                     /gene="bfr"
                     /locus_tag="PKL11_06045"
     CDS             complement(10197..10664)
                     /gene="bfr"
                     /locus_tag="PKL11_06045"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNZ13200.1"
                     /translation="MKGQEKILALLNDLLADELTAINQYIVHSEMCADWGYGKLHDAT
                     EHRAIEEMKHAEKLIARILFLEGTPIVSNLKKLTIGAAVDVQLKNDHASESGAIKAYN
                     DGVRLCIELGDGGTRELLEDILEDEEAHIDWLETQLDQIEQMGLPNYLLSQTA"
     gene            complement(10802..11407)
                     /gene="infC"
                     /locus_tag="PKL11_06050"
     CDS             complement(10802..11407)
                     /gene="infC"
                     /locus_tag="PKL11_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNZ13201.1"
                     /translation="MYQEGEFSISTTTFRVNETIRVPEVRLIGPAGENIGVVPIERAL
                     RIARDAELDLVEVAPGSSPPVCRVMDFGKFLYERTKKEREARKAQTKIEIKEIRMRPK
                     TNEHHRGFKTRDARRWLLEGMKVRVTVRFRGREIDYPELALEDLKEVAAELLDVAAIE
                     QPPTLEGKTMSMVLAPSRPGHKKSAAEAAAPAPTPEPPAEG"
BASE COUNT         2163 a         3387 c         3527 g         2417 t
ORIGIN      
        1 cgcggtcgcg ggcaatttcg gggttcacct gggggatcag gtggtaaaca tgctcagctg
       61 ccagataaat ttccatattg gagcatcctg tgaggaaaat aatcaaggga ttcgctaata
      121 ctatattgca tccgggctga ttctgcaaga gattaggccg acctttcagg gtccacaaac
      181 agggttaggt aagggcaagg cgcagttgaa tctgacccgg cccttacatt ggttgagctt
      241 ttttgtgaaa gccgtagagt ctctgcactc tggcattgcc cttctctctg aacggactat
      301 aattatttct atccccaaat gctgttcaag aggcgccctg atggatccgg taaacgccaa
      361 ctgccgcacg attgacgagt atatcgcccg tttcccggac gatattcaaa ccatcctggt
      421 cgaactgcgg gctgtcatcc gggcggccgc gccagaggcc gaagaaaaaa tcagctatca
      481 gatgcccact tttgcgctca acggcaacct ggtacacttc gcggttcaca agaatcatat
      541 cggcttctac ccggcgccga gcggcattga agccttcaaa tcagagctgt ctggctacgc
      601 ctgctccaaa ggcgccgtga ggtttcccat tgaccggccc ctgccgctgg acttgattgc
      661 caaaatcgtc cggtttcggg ttgctgaaaa catgaaaaag gcggcgcaga aaccgcgcca
      721 gaaagcaggc catcaacggt gagtaagctg attctctctg ccctcagaac atttttgatc
      781 ggcgcgcttg tcctgggtgt gctgctcttc ctccccgccc agacgctcaa ctactggcag
      841 gcctgggttt ttataattgt ctttcagacc gccgtgaacg caatcggcct ctaccttgcg
      901 ctcaaagacc cggccctgtt cgagcggcgt aaaaaagtgg gcccggcggc agagcaaaac
      961 cccgcgcaga agatcatcat gtctttggcg ctgctcagtt tggtagcctt gctggtggtc
     1021 tgcgcgctgg atcaccgcct ggggtggtct cgtatgccgg ttgtggcctc gctgattggt
     1081 gatctgctgg tcatgttggg gttatatctc aattttattg tgttcaaagc caacagcttt
     1141 ggcgcgtcca ccatccaggt ggttgaagcc caaaaagtca tctccaccgg cccttatgcg
     1201 cttattcggc atcccatgta tgggggcgtc ttgatcatgg tcgccggggt tcccctggcg
     1261 cttggttcat ggtgggggct gctgattctg gctctggtct tgcccgggtt ggtctggcgc
     1321 attcttgacg aagaacagac cctgaagcga gacttgccgg ggtacctgga atatatgcaa
     1381 aaggtgcggt accgcctggt gccttacctc tggtaattgg agcggcattt atcctcatga
     1441 cagcttccca gacaacccaa ccgccattaa ctcaggccga catcgaagcc ggattgcgcc
     1501 ggctgggcct tgaaagaggc cagattgttg aagttcacag ttcgctgagc cgttttggcg
     1561 atgtggcagg cggccccgct gcagtggtag atgccttgat gagcgtgatt ggcccgcaag
     1621 gcgcaatcgt catgtcggcc tatccggtca gcaagcccct gccgctttcc gaagcagaaa
     1681 agagctgggg ggtgctggct aaagtgcaga cttatcccga aagctatcgc ggtccaaccg
     1741 gcatgggtgt gattgccgac gaattttgcc gccggcctgg cacaattttg gggccgggct
     1801 ggcaccgggt ttgcgcctgg ggtcgagacg cgcaaaaact cagccagggc taccaggcct
     1861 tgctggcgct ggatggctgg gcgctgctgc tgggggtgga catcgagcgc ctttccagta
     1921 tgcaccaggc cgagaaagaa cccctgccgc ccgaaattac gcagtatgtt gcccccccaa
     1981 ccgaaattcg ccagcgctac cccgctgatt actatatcgc ttacggacag acgccggaga
     2041 atgcctggtt gaagatccag gcgcgggccg aaagtgagca gatcatcaag attggccaga
     2101 tcggccaggc tacctgccgg tttttcaaag cccgcccggt ggtcgagatg tatgcagagg
     2161 cgctgcgtac cgatccgtgg gggttgtacg gggtcaagaa aagcgcataa cctgtgtgca
     2221 ggagcggcac gggcatcaaa tttctgcgct ttgattttta ggaagcgccc ggaacgtgga
     2281 accccggaac gttgtataat cattttgccg gaggttgttt gccatgcttt tggatcgcct
     2341 ggatacgctg accgctcccg cctggctgag cgagcccgcc gagggcggcg cggtgcgctg
     2401 cctggcctgc gcccaccgct gcctcattca acccgggcgg cggggggtct gtcaggtgcg
     2461 cttcaaccgc gccggcgagc tgcgcgtccc ctggggctat gtggccgccc tgcaggatga
     2521 tccgattgag aaaaagccct ttgcccatct gctgccgggg gcgcgggccc ttacctttgg
     2581 catgttgggc tgtgattttc actgcgattt ctgtcagaac tggctgacca gccagacggg
     2641 ccgtgacccc gccgccgaac gcggggcgct ctacatcgaa cctgtgacgc ccgagcaggt
     2701 ggtgcgcgtg gcacagcggg tgggtgcgca ggtggtcgtt tcgtcttaca atgagccgct
     2761 catcaccacg gagtgggcgg tggatatctt caaactggtc cgggccgccg ggctgcgctg
     2821 cgcctatgta tccaacgggc acgccacccc ccaggcgctg gccgcgctgc gcccctacct
     2881 cagcgcctac aaggtggatc tcaagacgat gcaggctgag cagtaccgcg ccctgggcgg
     2941 ccggctggaa gccgtgcttg ccactatcca ggccgcgcac gacctggggc tgtgggtaga
     3001 ggtggtcaca ctggtgatcc ccgactacaa cgacagcccc accgagctgt gggaggcggc
     3061 gcgctttatt gcctcggtct cgcgggatat tccctggcat gtgaccgcct ttcacccgga
     3121 ttatcagatg accgaccggc cccccaccgg atcggaggcg ctgcagcgcg ccgccgaaat
     3181 cggtcaggaa gccgggctca actacgtcta tgcgggcaat ctgccggggc gggtcggctc
     3241 gctggaagac accttctgcc cacactgcca ggcgcggctg attgagcggc gcggctacac
     3301 catccgcgcc aaccgcatca catctgcggg cgcctgcccc aactgcggcg cagccattgc
     3361 cggggtgtgg taacgttctg cgttcccggt gccagggccg cacctgagac cgaataccca
     3421 aatcaaaacg cccagtattc gggtgaatac cgggcgttgg tgttatctac cgggcggcaa
     3481 aactactttt tgtctgccgg cggcgtgggt ttttcggctt cggggggctg cggtgcggcc
     3541 tcgggggttt gcggcatggc gcgccccagg taggcgggca gttcaatgcc agccatggcg
     3601 gcaatctctt gcaggggcgg aatggcgcgc acaaacccgc tcacaaagtc ggcggcggcg
     3661 ttgccgctgc cattgtggct gccgccatcc cagacagtga tcttatcaat ccgcatccgg
     3721 ctgatggcct caacctgcgc ctgaaccatg tcgctcagtt tctcaatgac cagcaggctg
     3781 gctgcggcct gggcatcgcc attgcagctt tcgatcaggc cgcggtaacc gacggctttg
     3841 gcttccagca gggtgcgcag accctcggcc tcagcctgat agcgcatcag gatgctgtcg
     3901 gcttcaccgc gcgcttcccg gcggacgcgc tcggcgttgg cttcggcgtc aatttcttgc
     3961 ttggtcttgt tgatttcctg ctgggcgatt tcttcagcct tcaacagggc catgcgggct
     4021 tcgtactcgg caatctgaat ggctttttca gcctcgcgtt tggcaatttc ggcccgctgc
     4081 atggcagtgg ccttctgcac ttccagctct gcgtttgagg cggcaatttc ggcccgcgag
     4141 ttgttttcgc cgcgcacggc ttcggcctcg cgctgctgca caaagatacg ctgcccggcc
     4201 atggcggccg tcttgccttt ttcggcttcg gccaggttct ctgagacgcg aatggtctcc
     4261 tcgcgcttgg catccgattc gccaatcgag cccttcttgg tctgctgggc cacatccacc
     4321 ttggcctggt tcaccgcctc cgaggcggcc ttcttgccga tgctttcgat gtaatcgctt
     4381 tcatcggtga tgtcggtgat gttcacgttg atcaggtaga ggccaatctt gttcagctcg
     4441 ggctccacgt ttttgcgcac ggcttccagg aagctctcgc ggtcctggtt aatctgctcg
     4501 atggtcagcg aggccaccgt cagacggagc tggccgaaga tgatttcttt ggccatggcc
     4561 tcaatcaggg caggggtcag gcccaccagg cgctcggcag ccaccgtcat gacctcgggc
     4621 tgggtgctga tgcccaccgt gaacgtgctg ggcacgttga tgcggatatt ttgcagcgac
     4681 agcgcatttt gcagcgggat gttgatggtc aggggggtca ggctcatata gcggtgttct
     4741 tgcgccaccg gccagataaa cgcgccaccg ccgtgcatac acctgctcga catgcccttg
     4801 cctacccgcc ccgaaatgac cagaatcttg tcggacgggc agcgcttgta acggctggcc
     4861 atatacagcg cggcaaagaa catcaagacg atgaagacaa tgatcgccag cgtcagcagt
     4921 ggaaaatcag acatccaaaa acctcctgaa gaaattatga gttttccggg ggctgttgtt
     4981 caaccaccac cagaatttta cccgcggtaa cgcccgtcac ccgcaccatg gcgccggttg
     5041 gaatgcgttt ccccgcttca gacatggcat ccagcgagcg cagggtgccc ttgaccgcca
     5101 ccgaaacaac ccccgtgccc tgttcgggga tggtcagata gaccgagccg atctgcccga
     5161 tggcatcttg aatgaccatg gtgccttcag attggagccg ccgcatttga tagaagatca
     5221 tggcagtcac ccaggcggtg accagcccgg cagccagggc gcccagcagg ctccagatgg
     5281 gtgcggcttt gacctgcagc agccccagcc ccaccagccc gaacatggtg aagaacccgg
     5341 ctatggactg cagcgagagc agcttcatgc cgatattgcc atcgggcgcg tccaccgagc
     5401 catcgtggtc caggtccaca ccgctgtcgg gcgtatcacc gtgatccaat ccgatgatca
     5461 tcatcaccag ccgcagcagc agaaccgtgc cgccgccgat ggcgcaaatc aaatagatgg
     5521 tatttaagcc cataaagcct cctgggggtg gccggtggat gaaaatgcgc cggcccgcaa
     5581 aataattata cccgaaacct gcggcagtgt tttgcgttcc gggttccagg tgcaggggtc
     5641 tgtgctgtga ccaggctgcg gtttggcgct cagcccactt tgcggcaggg gtaagtctcg
     5701 gggccctggg tcagcacaaa gccagtcgcc tggccctggg cgtcaaagct gtaatccagc
     5761 gtcagagctt ctacacgcgg gaagagccgc tcgggggttt cggcaaagag cgcaatcttc
     5821 tcctgccccg gtgcctgaag gtagagctgc ccgtcggtca gcgtgaccat cagggtcatg
     5881 ccgggggcca gctcaaacgc gcccacatac cgccccagcg cggcggggtc cagctcgatg
     5941 gcggtgcggg gctgcggcag gggacggtcg gttttgggtg cgcgctccac cgggcccagg
     6001 cgccgggtga tctgcatgcc gctgacgcga ccgtcggcag tgcgctcaaa gcaaaactga
     6061 tcgccagccg attctttgat aaaaaaggca tcttcagcat acgggtacag gcgcagccgc
     6121 ggcccgcccg tacgctgtga gtagagctgc ccatcttcac aggtaaccac ccgctccgct
     6181 ttgtcgttaa cggcatacac gcccacatag tctgccagca cctcggcggg cagatctatg
     6241 ggggtggggt cggcataggg cttgccgatg gccatggccg ccagcttgaa ggccaccagg
     6301 ttggggtcgt tgatagaatc atcctgattg gtcagcagcg ccacatagac ccccgcggcc
     6361 ggaacccgca ccgccccgca ggcaaagccg ttgatgccgc cgccgtgctc gatgaaggta
     6421 tcgcccgcat agtcccaaat gctccaaccg tagccatagc cggttgattc gccgttgttc
     6481 agatgatagg gctggaaggc cagtgccagg gtctcggggc gcagcagttc accggcggtc
     6541 agggcggcat tccagcgcgc caggtcgtcc acgttagaaa tcaaagcgcc agcagcgtaa
     6601 gggtgcgtca tgctcaggta gggggtgttg attaagccat ccaggccttt ggagtagccc
     6661 gccacccgcc ccggcaccag gcgcgcagta tcatcataca gggtgtgcgt caggcccagg
     6721 cggtcaaaaa tctgtgtctg taagaactgc gcgtagggca tcccgctgat tttctcgatg
     6781 atggccccca gcagaatata gccagaattg ttgtattgaa atttctcacc gggttcaaaa
     6841 tcaaagggct gatctttgaa cagggcgatt agttcatcca gggtcaagtc tttgcgccag
     6901 agggggtacc actcgggcag gctggtgtag cttttgatgc ccgaggtgtg cgtcaacagg
     6961 tgctcaatgg agatggtgcg cccgccggtg ggataatcgg gcaggtagcg ggtgaggtcg
     7021 tcttgcagcg agagcttgcc ctgttccagc agcatcaaaa tgcagaccgc cgtgaactgc
     7081 ttggtgaccg agccaatccg gaagatcatg gcggggtcca cgggtacgcc cagctccagg
     7141 tgcgccaggc cgtagccctt gcgcagcagc ggctgcccgg cttgcatcac aatcaccgcc
     7201 gcgccgggct ggtcagccgg gtacagcgct tgcagcagtt cgtccatctg gtgggatagc
     7261 tctaaatcgg tcatgggaag ctcctggatc ggcataagat gagggtgggg aatgctttga
     7321 ttatacccag aaggtgggcc tctgcgccgg atgattattg aaggatcatg gtatgattat
     7381 ttatcccctc aagcgtctga tttcaaatca tctcaaggag aaaccaacat gtccgggtat
     7441 tcgccaaatt tcaaccttcc tgcgtatggc aagtataagc aaagctcctc aaaaagctac
     7501 ctcatcaagt tgatcattat tgccgtggtt gcagcgggct tgctggcaat tatggatgtg
     7561 ctgctggggg tttttgtggg cgtggtgatt attgcggggg gcctgttcca aatgtccaaa
     7621 agcagccggg gctgggatgg cgcgatccag gccaaagatc agatggcatt ctccgacact
     7681 cagcccgatg gcaccatcaa tacctacacc aaatacgagc tgaaggtgga acgcgcaccc
     7741 ggcaaagttg aaaccctcaa atggactgac aattcggggg cgtttgaata ttatcgcgtg
     7801 ggcgagcagg tgcggcacca ccccggctgc gatttccccg agaagtttga taaatccaac
     7861 gatgaacagg tgatctgcgt ggcctgcggc gagctgtatg atctgccaac cgaaacctgc
     7921 cccaaatgcc aggtgccgct gttgaagtga aattggactg aatatccccg aggcttgaag
     7981 atggacgatc tctctgacat tgccgaatat tacaacaccg acccgcagca cgaggcggac
     8041 cggctggacc gccaccaact ggagctggac ctgacctggc gctatctgga gcgccacctg
     8101 ccgtccgcgg ccaaaatcct cgaaattggc gccgcaaccg ggcgctacac cctggagctg
     8161 gcccgccgcg gttataccgt gaccgccgtg gatctttcgg caggattggt ggagcactgc
     8221 gccgcgcggc tggctgaagc cgggcttgcg gcgcgggtgc ggctggtggt ggctgacgcg
     8281 cgcgacctga gcgccatcac cgagacggat tatgacgccg tgctgttgat gggcccgctc
     8341 tatcatctga tgctggaagc agaccggctg ctggtcttgc agcaggttta tgcccggctg
     8401 cgccccggcg ggttgatctt ctcggcgctg atcagccggt acggcattct gggcgacttg
     8461 atccgcaaca ttcccgactg gattgacgac cccgaggtgg cctcgtttat ggcgcgcggc
     8521 cgccgcccgg atgattatcc ccccggtggg tttcgcggct attttgcccg ggttgaagaa
     8581 atcatcccgc tgcacgccgc cgccgggttt gaaacgctga ccctggccgg ggtggagcca
     8641 gccatctcgg cggatgatga gagttacaac cggctggtag gcccccagcg gcagaaatgg
     8701 ctggacctgc tggaagccgt cagcgccgag cccacgctgg tgggggcctc gcggcatttg
     8761 ttatacatcg ggcgcaggcc atgacgcccg agatcatcat cggcattgtg ggctcggccg
     8821 cgctgctgtt cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccatgac
     8941 gcccgagatc atcatcggca ttgtgggctc ggccgcgctg ctgttcatct cacgggctgc
     9001 gctgcgcacg ccgggcgcgc acggctttta tcgttttttt gcctgggagg gcctgctgat
     9061 tctgtttctg ctgaacgctc gccggtggtt tgtgcagccg ctggcgctgc atcagatcat
     9121 atcgtggggg ctgctgctca tttcgctggg gctggtgctg gctggcctca gcctgctgcg
     9181 ccgccggggg cagccagacc cgcagcgcgc cgatgaggcc ctgctgccgc tggaaaaaac
     9241 caccgccctg gtgaccaccg gcatctatcg ctacatccgg cacccgctgt acagttcgct
     9301 gctgtttttg ggctggggca tcttcttcaa gcagccggcg gggtggggcg cggcgctggc
     9361 gctgggggtc agcgtgtttc tggtgctcac cgcccgggcc gaagagcgcg aaaacctgcg
     9421 ctatttcggc gcggcatacc aggcttaccg gcagcggacc aagatgttcg tgccgtttat
     9481 tttttagcca cgggaggcat ttaatgaaaa aactcaccct gattctctta ctcgctctga
     9541 cgctgctgct ggcggcctgc ggcggcggca aagaccagcg gccccacagt gagacggtgg
     9601 ctgaagataa ggtggtggac ctggcctgcg gggcgctgag ccgcaaagac tgcctggcgc
     9661 gcctctgcgc tgaccccgcc gcctgcccgg tgctgattgc cctggctgaa cccgcagtat
     9721 ttgattttat tgccacctat gccgcctgcc ccgactgcaa cacgcccgat tttgcccccg
     9781 aagatggcat tggcaagtgc gtggaatatc aaacctccga cgccccctcc ggctgggagg
     9841 tgaccttctg ggtctcggag aattgcacat tccgctacgg cagccccacc gagagccggg
     9901 tggtggtgcg catcaacccg cagacgctgg agatcaaaaa gatcagcccg tcggtcgaga
     9961 tcatcgccga cccgctgtac tgccagaaca aggccgactg cgtcagcctg tcgggcagcg
    10021 gggtgccgtt caccgggtgc agcaattcgt tctacggccc gctgaactgg agcggctact
    10081 acgccggaac cgaatgcacc tgccagaagc atcaatgcgt ggcagcggcg cctaaaaaat
    10141 aaaccgggca gccttttggg gccgcccggc aaagggtaga tccaggtgaa ccggacttac
    10201 gcggtctggc tcagcaggta attgggcagg cccatctgct caatctgatc gagctgggtt
    10261 tccagccagt caatgtgggc ttcttcgtct tccagaatat cttcgagcag ttcgcgggtg
    10321 ccgccgtcgc ccagttcgat gcacaggcgc acgccgtcat tataggcttt gatggccccg
    10381 gactcggaag catggtcgtt tttaagctgc acatctacag ccgcaccaat ggtcaacttt
    10441 ttgaggttgc tgacaatggg cgtgccttcg aggaacagaa tgcgggcgat cagtttttcg
    10501 gcgtgcttca tctcttcaat cgcgcggtgc tcggtggcat cgtgcagttt gccgtaaccc
    10561 caatcggcgc acatttcaga atggacgatg tactggttga tggcagtcag ctcgtctgcc
    10621 agcagatcgt ttaagagtgc aagaattttt tcctggcctt tcatgggaac ctcctgaaga
    10681 atttgtgaaa cgaaacagcg agacagaaag attgtagcac aaaatctgac cgccgggcgc
    10741 ggccggatgg cggtgcttaa aaacaaactt gcgaagcagg ttgcttcgca agtgaatggg
    10801 tttatccttc ggcggggggc tcgggtgtgg gagcgggggc cgcagcctcg gcagcggatt
    10861 tcttatggcc cgggcggctg ggcgccagca ccatgctcat ggtcttgcct tccagcgtgg
    10921 ggggctgctc aatggcggcc acgtccagca gctcggcggc aacctctttg aggtcttcca
    10981 gcgccagttc ggggtagtca atctcgcgcc cacggaagcg cacagtgacg cgcaccttca
    11041 tgccttccag cagccagcgg cgggcatcgc gggttttgaa gccgcggtgg tgctcgttgg
    11101 tcttgggccg catgcgaatc tctttgattt cgattttggt ctgcgccttg cgagcttcgc
    11161 gctctttctt ggtgcgctcg tagaggaact tgccaaagtc catcacccgg caaaccggcg
    11221 ggctagagcc cggcgcaacc tcaaccaggt ccagctcggc gtcgcgggca atccgcagcg
    11281 cacgttcaat tggcacaacc ccaatgtttt cacccgccgg gccaatcaag cgaacttctg
    11341 gcacacggat ggtttcattt acccgaaaag tggtagtact gatggaaaat tctccttctt
    11401 gatacaaatg aacaactgcc gtcggcgtta cggcataaac taggatgata ataccttaaa
    11461 ttgtcgaaaa cgtcaattta acggggattg ggtttcaggt tttgggccgg gtacgttcca
    11521 gatgcggaca gacggtacca cgttccaagt tccaggtgcg aggggaccgt ctggcgtgaa
    11581 gcgtaggggt cgcg
//
LOCUS       DAOPVS010000053        11475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_5748_length_11475_cov_52.753765, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000053 DAOPVS010000000
VERSION     DAOPVS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11475
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5748_length_11475_cov_52.753765"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKL11_06055"
     CDS             complement(<1..346)
                     /locus_tag="PKL11_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13202.1"
                     /translation="MTKPIIVALVAGAVAAFVFALIGPGAVWISCLAGGGLTTLAVWG
                     LFHVWRWAGGRRSLAWILILAFVLRLGVGIALHLLLPIYGDDEPVQNAGYVFYDAWQR
                     DQQSWQLAQSDQA"
     gene            complement(339..1490)
                     /locus_tag="PKL11_06060"
     CDS             complement(339..1490)
                     /locus_tag="PKL11_06060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ13203.1"
                     /translation="MRIAVISTSPVPSTTAHSIQMMKAAYALSQVDSPVRLWTPGSEA
                     APWPKLSQQYGIIQPFEITWVPSRPQLRRVDFVTTSVGQVRQWGADVLYTWTVQAAVA
                     ALLQHIPVILELHDRPTGFFGKSWLRLFASLSGEKRVLMITHALRTALETEFNLIFPD
                     SVVQIAPNGCDLEQYAAMPDAPTARQRLALPERLTAVYSGHLYSGRGMDILLALASAY
                     PEVQFIWAGGKPESVKEWQHRIEKESLDNIYLTGFLDHQQLPLMQSTADILLMPYATR
                     IAGSGGGNSADICSPMKMFDYLATGRAIMTSDLPVIHEILTPACAVFCPPEDESAWVQ
                     AFGDLVADAAWRSRLGACAQAQAAKHTWRARASRALADFPSGESADEID"
     gene            complement(1494..2867)
                     /locus_tag="PKL11_06065"
     CDS             complement(1494..2867)
                     /locus_tag="PKL11_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ13204.1"
                     /translation="MTSSPPASPPGRWIKDRLLGKVVKNSTYLFASNAISAILSIVTA
                     NLLGVSNFGVLGLVIGFVSDVNRLFSFRMGEVIVRYVGEALEHQNRERAAAVIKAAAL
                     AEAATSLLAFGVLVLLAPLGARLFIKDAAAVPLILLYGITILGSLIAETSNGILQVSG
                     HFRSQAVLNLIQAVVTAALIILAAVQKSGVLAVLWAYLAGKLIIGLGPAVLAWRHLPG
                     LLGSNWLRTPFKHLPPLRELLRYAISTNFSGTINMIARDSEVMWVGFFFTSVEAGYFK
                     MALSIVNLMIMPITPFISTTFPEITRSVASRSWQRLRSLLGRVSIIAAGWTAAVVLGL
                     LLLGRQLLFEPLFTLAGHTFQLYTADYLPAMPVLFILLIGFGIANILFWNRPLLLALG
                     QPEYSLKVGFWCMLVKVLLAVILLPAAGYLTEAALLSGYFLISVSWMVWRGLKTLRQL
                     EQELLKG"
     gene            complement(2864..3841)
                     /locus_tag="PKL11_06070"
     CDS             complement(2864..3841)
                     /locus_tag="PKL11_06070"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ13205.1"
                     /translation="MARYLVTGAAGFIGAQVSTQLIADGHQVVGVDNLNDAYDVRLKH
                     YRLEHLQKLADFSYRHIDICNLPPLKEMFAQDGPFDAVVNLAARAGVRFSVENPWSYL
                     DTNTTGALNLLDLCRQYQTPKFVQASTSSIYGANAPLPTPEDADSSLPLQPYAATKKA
                     AEVLAHSYHHLHGLDVTVVRYFTVYGPAGRPDMVMFRFAQWIAEGQTVRLNGDGNQSR
                     GFTYVDDIARGTILALKPVGYEIVNLGGHEVITINELIAQLEQRIGRSARVERYPFHP
                     ADMLSNLADVSKARQLLGWNPQISLSEGITNLVDWYMTERSWASQVLTP"
     gene            complement(3841..5154)
                     /locus_tag="PKL11_06075"
     CDS             complement(3841..5154)
                     /locus_tag="PKL11_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNZ13206.1"
                     /translation="MDTQQELIHQLQTRTAKVAILGLGYVGLPLAVVFAEAGFEVIGI
                     DPIAEKVEMLNRGESYIIDVPTETVKRLVRLGKLSATTDFKALQQAQAVSICVPTPLS
                     KFGDPDLSFIASAVESVTQNIHPGMVIVLESSTYPGTTREMVLPKLEQSGLKAGLDFY
                     LAFSPERVDPGRTDWTTINTPKVVGGITANCCEVATAWYAQALQKVVTVSSAEVAEMA
                     KLLENTFRMINIGLVNELAIMCDRLGVDVWEVIDAAATKPFGFMKFTPGPGLGGHCIP
                     IDPLYLSWKLKSLNYTARFIELASEINANMPRFAVGKVQDALNDQGKALRGAKVLVLG
                     AAYKPDIDDLRESPALDVIGLLQKKGAQVSYHDPYIPHLHHDTIQLDSVSDLMAAVRS
                     VDCVVIVTNHSTYDYTAILETASLIVDTRNALGDRGRNHPKVVRL"
     assembly_gap    5187..5286
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5322..5969)
                     /locus_tag="PKL11_06080"
     CDS             complement(5322..5969)
                     /locus_tag="PKL11_06080"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNZ13207.1"
                     /translation="MNSAEALQEVARQVAVCEQCALHFSRKRAVPGEGPGNAEVLLIG
                     EGPGFYENEQGRPFVGQAGKFLDELLQSAGFKRPDVFITNVVKCRPPGNRDPQPEELV
                     ACSSYLDRQIEAIQPRVIVTLGRYSMARFLQNAKISEVHGQAVWVRGRLIVAMYHPAA
                     ALHQPSLKPQLEQDFARLPEFIEQARSSTPPTADASDSASDQSPADDKPTQLSLF"
     gene            complement(5966..6499)
                     /gene="rimI"
                     /locus_tag="PKL11_06085"
     CDS             complement(5966..6499)
                     /gene="rimI"
                     /locus_tag="PKL11_06085"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNZ13208.1"
                     /translation="MDVIIRPMLLEDVERVYEIDCLSFSLPWPERSYRFELTQNPNSH
                     PWVAVVKDVLGQETLVGMIVLWLVIDEAHIGTIAIHPDWRRHGIAQRLLAHALLHAVT
                     LGVDRVMLEVRRSNLGAQALYNRFGFVEDGVRRHYYRDNGEDAILMSLAPLQSAAVQA
                     ALAELNAAQCFNEEASQ"
     gene            complement(6512..7171)
                     /gene="tsaB"
                     /locus_tag="PKL11_06090"
     CDS             complement(6512..7171)
                     /gene="tsaB"
                     /locus_tag="PKL11_06090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNZ13209.1"
                     /translation="MDTSTQWVGLALYDGAQVLGEITWQTHSHHTMEMAPAIQDLLQR
                     CGVRSDMLKALGVALGPGSFTSLRIGLALAKGLSLSLHIPVVGLPTLDILAAAQPVQD
                     LPLIALLQAGRGRLAVGHYKPGRGRWKPQDEAVLQTLDQLVESINDQPALVCGELDAA
                     ARQTLSRQCKSAVLASPARSLRRPAVLAELAWQRWQTDKVDDVVSLAPIYLRVADPIP
                     G"
     gene            complement(7185..7679)
                     /gene="tsaE"
                     /locus_tag="PKL11_06095"
     CDS             complement(7185..7679)
                     /gene="tsaE"
                     /locus_tag="PKL11_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNZ13210.1"
                     /translation="MPILDLHTLEFFSRSPEQTRRVGMRLGALLQRGDLICLSGDLGA
                     GKTTLVQGIAQGWGTLDAVSSPTFVLANLYRRSDGQQLHHLDAYRLQNAQEAEDLDLD
                     QMLETGALIIEWPERILSALPADRLWVQLRWLADEQRGLVFTPAGSRFEQMVDELRHR
                     LVGG"
     gene            complement(7688..7900)
                     /locus_tag="PKL11_06100"
     CDS             complement(7688..7900)
                     /locus_tag="PKL11_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNZ13211.1"
                     /translation="MLPDLKQNDIVRLRKPHPCGSYDWKITRLGADIGLECCGCARRV
                     LLSRRELMRRMKQVLPSNQDSEANEV"
     gene            7945..8586
                     /locus_tag="PKL11_06105"
     CDS             7945..8586
                     /locus_tag="PKL11_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNZ13212.1"
                     /translation="MEPAILITYAITLIISISVHEFAHAWSADRLGDNTPRMYGRVTL
                     NPLAHLDPMGSLMMIFAGFGWGKPVPVNAFALQRRSPAALMLTAIAGPLSNLLLAILA
                     AIPFRLKLVHIAFDTSEAIFPSIDIFLTYFILVNLSLMLFNLIPLSPLDGDKIADYFF
                     PPFMAKALASIRPYAPFVLMGLVFLPRFLGIDILGEIMSPAISNIWMLLMGVY"
     gene            8586..9581
                     /locus_tag="PKL11_06110"
     CDS             8586..9581
                     /locus_tag="PKL11_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNZ13213.1"
                     /translation="MTIQLTIIGLGQVGASLGLALQEQKERIHRTGLDRSGDVTVKAH
                     KIGAIDRGARNAPDAVKDADVVILATPVDELRETLEVIGPLLREGAVVVDTSALKIKA
                     AEWAKEFLPAGRHFIGMMPTLNPAYLHETQTGIDAAHADLFHNSLMVIASFPDTHPDA
                     VKLASDLSQLLHSKALFTDPYEADGLTAASELLPQLAAAALVHSIQSQPAWREGRKLA
                     SRAYAEATEPVLGLNENEAPGVTALNNRENTLRVLDDLIATLNEMRAVIDSQDSEQLA
                     KILDKARQDRREWWAQRVRAEWDKIDNVQTLSASEAISGIFGFRRKAKEKTRNRR"
     gene            9603..9857
                     /locus_tag="PKL11_06115"
     CDS             9603..9857
                     /locus_tag="PKL11_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNZ13214.1"
                     /translation="MAFYCYILECADGSFYTGWTTDPVRREKQHNRGTGAAYTRTHRP
                     VRLVYVEPQASRSDAMRRERAIKRLTHLRKEALIHGQSKS"
     gene            9838..11001
                     /gene="galK"
                     /locus_tag="PKL11_06120"
     CDS             9838..11001
                     /gene="galK"
                     /locus_tag="PKL11_06120"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNZ13215.1"
                     /translation="MANPNRNLLLEQATLTVIAPGRVNLLGEHVDYNDGLVLPAAINR
                     HVRLEAKPRADRLVVLHAKDLEETVCFELDGLEKRQTSGGEPLPHWALYPAGVAWAAQ
                     QRGLPVQGIEAAYTSDVPIGAGLSSSAAVEVGFAALWRSLGGWALNNLMLAQLSQQAE
                     NQYVGVNCGLMDQFASANGVEGHGLLFDTRSLDFRPLPLPGGTVIVIADSGVRRRLVG
                     SAYNDRRAACEQAVALLKKELPGIRALRDVSRDDFNRLADHLPPIVARRARHIVEEID
                     RVDAAIGMLSQGDAAGFGRLMLATHASLRDLYEVSIPELDALVEIAARQPGCWGARLT
                     GAGFGGCTVNLVQQDRAEAFMVALKNDYTKNTGQQAEVYLCQASRGVHVEPSG"
     gene            complement(11005..>11475)
                     /locus_tag="PKL11_06125"
     CDS             complement(11005..>11475)
                     /locus_tag="PKL11_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNZ13216.1"
                     /translation="RIAILNILGDTELVQACAKALAEKMESVVYDVVVTAESKSIPLA
                     YAISVVTRKPYVILRKAYKPYMGQALQAETLSITTGQPQTLYLDEKDLRVLEGKRVVI
                     LDDVISTGSTLQGMRLLMQKAGAQVVAEAAILTEGERARWADIISLGHLPVFTD"
BASE COUNT         2711 a         2968 c         3386 g         2310 t
ORIGIN      
        1 gggcctggtc ggattgcgcc aactgccagg actgctgatc acgctgccag gcatcataaa
       61 aaacatatcc ggcgttttga acgggttcgt catcgccgta aataggcagc aacagatgca
      121 gggcaattcc aacacccagg cgaaggacaa aggccagaat caggatccag gcaagggagc
      181 gcctgccgcc agcccagcgc cagacatgaa agagccccca aacagctaaa gtagtcaggc
      241 caccgccagc aaggcaagaa atccaaacag cgcccgggcc gataagtgca aaaacaaatg
      301 ccgccacagc accagcgacg agggcaacga taataggttt agtcaatctc atcagcagat
      361 tctccagagg ggaaatcggc gagggcacga ctggcgcggg cccgccaagt atgttttgcg
      421 gcctgggcct gggcacaggc gcccaaccga ctacgccagg cggcatcagc aaccagatca
      481 ccaaaggcct gaacccaggc agattcatct tcaggtgggc aaaagactgc gcaggcaggc
      541 gtcaagatct catgaattac aggcaggtca ctggtcatga tagcacggcc agtggccagg
      601 taatcaaaca tcttcatcgg gctgcagatg tcggcagaat taccgccgcc gctgccggca
      661 attcttgtag cgtaaggcat caataaaatg tcggccgtgg actgcatgag cgggagctgc
      721 tggtgatcta aaaagccggt caggtagatg ttatccaatg attctttttc aatcctgtgc
      781 tgccattcct tgacggattc gggttttccc ccggcccaga tgaactgaac ctcggggtaa
      841 gcgctggcca gagcgagcaa tatatccatg ccacgtccac tgtataagtg cccactgtaa
      901 acagcggtca aacgttcggg caaggccagg cgctgccgag cggtgggggc atcgggcatg
      961 gcggcatact gctccaggtc gcagccattg ggggcaattt gcacaacaga atcgggaaag
     1021 ataagattaa attccgtttc cagagcagta cgcagcgcgt gggtgatcat caggacgcgc
     1081 ttttcaccgc tcaggctggc aaacaagcgc agccaggatt tgccaaaaaa gcccgtggga
     1141 cggtcgtgga gctccagaat taccgggatg tgttgaagca gcgccgccac tgccgcctga
     1201 acagtccagg tgtagagaac atctgcgccc cactgtcgaa cctgaccgac agaggtggtc
     1261 acaaaatcaa cccgccgaag ctggggacgg ctgggcaccc aggttatttc aaagggttgg
     1321 atgatgccat actgctgaga tagtttaggc cagggagccg cctctgaccc gggcgtccag
     1381 aggcgcacgg ggctatccac ctgagacagt gcataagcag ccttcatcat ctggattgag
     1441 tgggcggttg tagaaggaac cggagaggtg gaaataacag ctatacgcat tatttaccct
     1501 ttcagtagct cttgttccaa ttgacggagc gttttcaaac cacgccagac catccagctt
     1561 actgagatca ggaaatatcc cgacaacagg gcggcttcag tgaggtatcc ggcggcagga
     1621 agcaggatga ctgcaagtaa aaccttaacc agcatacacc aaaaacctac tttgagcgaa
     1681 tattctggct gccccagggc taaaagcagc gggcggttcc agaaaaggat attggcaata
     1741 ccaaaaccaa tcaatagaat gaaaagcacc ggcatggcgg gcaggtaatc ggcggtatag
     1801 agctggaagg tatgcccggc aagggtgaat aagggttcaa ataataactg ccgccccagc
     1861 aacagcagac ccaacacaac agcagccgtc caaccggcag cgatgatgct gacgcgcccc
     1921 agaagtgagc gcagacgctg ccaactgcgg gaagcaacgc tgcgggtgat ttccgggaag
     1981 gtggtactga tgaacggcgt gatgggcata atcatcagat tgacaatgga taaggccatt
     2041 ttgaaatagc cagcctcaac cgaggtgaag aagaagccga cccacatcac ctcgctgtcc
     2101 cgggcaatca tattgattgt gccagaaaag ttggtggaaa tggcgtagcg cagcagttcg
     2161 cgaagtggag gaaggtgttt gaatggagta cgcagccagt tggaaccgag cagcccaggc
     2221 aaatgacgcc aggccagcac agccggacca aggccaataa tcagtttgcc agccagatac
     2281 gcccagagca ccgccaggac gccggatttt tgaaccgcag ccagaattat caacgctgcg
     2341 gtgaccacag cttgaatcaa atttaatacg gcctgactgc gaaagtgccc gctgacttga
     2401 agaatgccat ttgaggtttc ggcaatcagg ctgccgagga tggtgatgcc ataaagcaga
     2461 atcaggggca cggcagcggc atctttgatg aatagacgcg caccaagagg agcgagcaag
     2521 accaaaacgc caaaagccaa taaagaagtg gccgcctcag ccagggcagc ggctttaatt
     2581 actgctgcgg cgcgctcgcg attctgatgt tccaacgctt cgccaacgta acggacaatc
     2641 acctcgccca tgcgaaaaga aaagaggcgg ttcacatcgg agacgaagcc aatcaccaac
     2701 cccagtacgc caaaattaga cacgcccaaa aggttggccg tgacaatgct caaaatggcg
     2761 ctgattgcat tgctggcaaa caggtaagtt gagtttttta ccaccttgcc gagcaagcga
     2821 tcttttatcc accgtcccgg aggtgaggcc gggggggagg aggtcatggc gtcagtacct
     2881 gtgatgccca gcttcgctcg gtcatgtacc agtccaccaa gttggtgatg ccttcagaca
     2941 ggctgatttg cgggttccaa cccaataact ggcgggcttt ggatacatcg gccagattag
     3001 aaagcatatc ggcggggtgg aaggggtaac gttcaacccg ggccgagcgg ccaatacgct
     3061 gctccaactg ggcgatcagt tcattaatcg taatcacttc gtgcccgcca agattgacga
     3121 tttcataacc gaccggcttg agggccagga tggtgccgcg ggcaatatca tccacatagg
     3181 tgaagccgcg cgactgattg ccatcgccat tcaggcgaac ggtttggcct tcggcaatcc
     3241 actgggcaaa gcggaacatg accatgtcgg ggcgaccggc ggggccatag accgtgaagt
     3301 agcgtacaac ggtaacatcc aacccgtgga ggtggtgata gctgtgcgcc aaaacctcgg
     3361 cggccttttt ggtggcagca tagggttgga gtgggaggct actgtcagcg tcttcgggtg
     3421 tgggcagcgg cgcgttggcg ccataaatac tggaggtaga tgcctgtaca aacttgggcg
     3481 tttgatattg acggcagaga tccagtaaat tcaaggcgcc ggttgtgttg gtatctaaat
     3541 aagaccaggg gttctcgact gagaaacgca cacctgcgcg agcggccaga ttgacgacag
     3601 catcaaacgg gccatcctgg gcaaacattt ccttcagcgg gggcagattg cagatatcaa
     3661 tatggcggta ggaaaaatcc gccagtttct gaaggtgctc caggcgataa tgcttcagac
     3721 gaacatcgta ggcatcattg agattatcca ccccaacaac ctggtgacca tcggcaataa
     3781 gctgggtgct gacctgggcg ccgataaaac cagcagcccc tgtaaccaga taacgtgcca
     3841 ttagagcctc actaccttcg ggtgattgcg gccgcgatca cccaaagcat tacgagtgtc
     3901 cacaatcaga ctggcggtct caagaatggc ggtgtaatca taggtggaat ggttggtcac
     3961 aatgaccaca cagtcgacag accggacggc agccatgaga tcagaaacgc tatccaactg
     4021 gatggtgtcg tgatgcagat gggggatata ggggtcgtga taagagacct gggcgccttt
     4081 tttctgcagc aagccaatca catccagggc gggcgattcg cgcaggtcgt caatatcggg
     4141 tttataggcg gcgcccaata ccagcacctt agcaccccgg agggctttac cctgatcatt
     4201 caaggcatct tgcactttgc caacggcaaa gcgcggcatg ttggcgttga tttcagaggc
     4261 cagctcgata aaccgggcgg tatagttgag ggatttgagc ttccaggaca ggtaaagcgg
     4321 gtcaatgggg atgcagtgcc cacccaggcc ggggccgggg gtaaatttca taaagccaaa
     4381 gggtttagtg gcagcggcat caatcacttc ccaaacatcc acacccaggc ggtcgcacat
     4441 aatggctaac tcgttgacga ggccaatgtt gatcatgcgg aaggtgtttt ccagcagctt
     4501 tgccatttcg gccacttcag cagaggagac ggtgaccacc ttttgcagag cctgggcata
     4561 ccaggcggtt gcgacttcgc agcaatttgc ggtgatgccg ccgactactt tgggtgtatt
     4621 gatggtcgtc cagtcggtgc gaccggggtc tacgcgctcg ggggagaagg ccaggtagaa
     4681 atccagacca gctttgaggc cagactgctc taattttggc aggaccattt cccgcgtggt
     4741 gccagggtag gtgctggatt caaggacgat aaccatgcca gggtggatgt tctgggtaac
     4801 cgattctacg gcagaggcga tgaaggacaa gtcgggatcg ccaaatttac tgagcggggt
     4861 aggtacgcag atgctgacag cctgggcctg ttgaagagcc ttgaaatcgg tggtggcact
     4921 taatttgccc aacctaacca ggcgcttgac cgtctcggtg ggaacgtcaa tgatgtagct
     4981 ttcgccacga ttgagcattt ctactttttc ggcgatggga tcgatgccga tgacctcaaa
     5041 accagcctcg gcaaagacca ctgccagagg cagacccaca tagcccaggc ccaaaatggc
     5101 aactttggct gtgcgggttt gaagttgatg aatcagttct tgttgggtat ccatatttct
     5161 ccggagttaa aataggctca actgagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnatca gttcttgttg ggtatccata tttctccgga gttaaaatag gctcaactga
     5341 gtgggtttat cgtcggcggg ggattgatcg gatgcgctat cagaggcatc ggccgtgggt
     5401 ggcgtggagg agcgagcctg ttcaataaat tctggcaggc gggcaaaatc ctgctccagc
     5461 tgcggcttca gggatggctg atgcagggca gcagcagggt gatacatggc tacaatcagg
     5521 cggccgcgca cccagacagc ctggccgtga acttcgctga ttttagcatt ttgcaggaac
     5581 ctggccattg aatagcggcc cagggtgaca atcacacggg gttgaatggc ttctatctga
     5641 cgatcaaggt agctgctgca ggcaaccagc tcttcgggct gcgggtcgcg gttgccgggg
     5701 ggacggcatt tgaccacgtt ggtaataaag acatcggggc gcttaaaacc agcagactgt
     5761 aataattcgt ccaagaattt gccggcctga cctacaaagg gccgcccctg ttcgttttca
     5821 taaaaaccgg ggccttcacc aatcaaaagc acttcggcat tgccggggcc ttcaccgggg
     5881 acagcgcgct tgcgcgagaa atgcaaggcg cactgttcgc aaaccgcaac ctgacgggcg
     5941 acttcctgca gcgcttcagc agaattcatt gggatgcctc ctcgttaaaa cattgcgccg
     6001 cattcagttc tgccagtgcg gcctgcacag cggcgctctg aagcggtgcc aggctcatca
     6061 aaatagcatc ttcgccgtta tcacgataat agtggcggcg aacgccatct tccacaaaac
     6121 caaagcggtt ataaagcgcc tgtgccccca gattggaacg gcgcacctca agcatgacac
     6181 gatcgacacc cagggtcact gcgtgcaaga gagcatgtgc cagcagccgc tgagcaatgc
     6241 catgccggcg ccaatcgggg tgaatggcaa tggtgccaat gtgagcttca tcaatgacca
     6301 gccagaggac aatcatgcca accagagtct cttgacccag aacgtcttta accaccgcca
     6361 cccaggggtg agagttgggg ttctgggtca gctcaaagcg ataggaacgc tccggccagg
     6421 gcaagctaaa ggaaaggcag tcaatttcat agacgcgctc aacatcctcc aggagcatgg
     6481 gccgaatgat tacatccatg cgtgatgacc tttatccggg gatggggtct gccactcgca
     6541 ggtaaatggg ggccagggag accacatcat ccaccttgtc ggtctgccag cgctgccagg
     6601 ccagttcggc aaggactgcc ggacggcgaa gggaacgcgc tggagaagcc agaactgcag
     6661 atttgcactg ccgggatagc gtttgccggg cagcagcgtc tagttcaccg caaaccaggg
     6721 cgggctggtc gttgattgat tccacaagct gatccagggt ctggagaacg gcttcgtcct
     6781 gcggcttcca acggccccgg ccaggtttgt aatgcccgac agccaaacgc ccgcgtccgg
     6841 cctgcagcag agcaattagc ggcaggtctt gaacaggctg agccgcggcc agaatatcca
     6901 aggtgggcaa gccaacaacc gggatgtgca gcgagagcga taagcccttg gccaacgcca
     6961 ggccaatgcg taagctggtg aaagagccgg ggcccagggc gacgccgagg gctttgagca
     7021 tgtctgagcg aacgccgcag cgctggagca aatcctgaat agcaggggcc atttccatgg
     7081 tgtgatggga atgggtttgc caggtgattt cacccagcac ctgagcgcca tcgtaaagcg
     7141 ccaatccaac ccactgagtt gaggtatcca ttgccagcag catttcagcc tcccaccaag
     7201 cggtgacgca gctcgtccac catctgttca aagcggctgc cggcgggggt aaacaccaga
     7261 ccacgctgct cgtcagccag ccagcgtagc tgtacccaga ggcggtcggc gggtagagca
     7321 gagaggatgc gttcgggcca ctcaataatc agagcgccgg tttcaagcat ttgatccagg
     7381 tccaaatctt cggcttcctg ggcattctgc aggcgatagg catccaaatg atggagctgc
     7441 tgaccgtcgg accgacggta taaattcgcc aaaacaaatg tcgggctgga aacggcgtcc
     7501 aaggttcccc aaccctgggc aatcccctga accagggtgg ttttgccagc acccaggtca
     7561 cctgagaggc agatcagatc gccgcgctgc agaagtgcgc caagccgcat gcccacgcgg
     7621 cgggtctgct cggggctgcg gctaaaaaat tctagagtat gcaaatccag aatgggcatg
     7681 tcaaaaatta tacctcattc gcctcagaat cttgattgga gggcaagacc tgtttcatgc
     7741 ggcgcatcaa ctcgcggcgg ctgagcagta cccgccgggc acaaccacaa cactccaggc
     7801 caatatccgc ccccagccgg gtgattttcc aatcatagct gccacaaggg tgcggcttgc
     7861 gcagccgcac aatgtcgttt tgcttgagat cgggtaacac gttcagatta taccacccat
     7921 gttataatcg cttttatgct aaacatggaa cccgctatcc tgattacgta tgcgattacc
     7981 ttgatcatct caatttcggt gcatgagttt gctcatgcgt ggtctgcgga tcgattgggc
     8041 gataatacac ctcgtatgta cgggcgcgtc acgctaaacc cgctagcaca tctggacccg
     8101 atggggtctc tgatgatgat atttgccggg tttgggtggg gaaaacctgt gccggtcaat
     8161 gcgtttgcac ttcaaagacg atcaccagcg gcattgatgc tgactgccat tgcggggccg
     8221 ctttcgaatt tgctgctggc tattttggcg gcgatcccct tccggttgaa attggttcac
     8281 atcgctttcg acacaagcga ggctattttc cccagtatag atatttttct gacgtatttt
     8341 attctggtaa atttgtcttt gatgttgttc aatttgattc cgctatcgcc gctggatggc
     8401 gacaaaattg cggattattt cttcccgcca tttatggcaa aagcattagc cagcatccgg
     8461 ccctatgccc cgtttgtgct gatgggcctg gtgttcttgc cgcgtttttt ggggattgat
     8521 attttggggg aaatcatgtc tccggctatt tcaaatattt ggatgctatt gatgggagtt
     8581 tattaatgac cattcaactg accatcatcg ggttgggaca ggttggggcg tcgctggggc
     8641 tggcgctgca ggagcaaaaa gagcggattc accgcacggg gttagaccga tctggcgatg
     8701 tgacggttaa ggcgcacaaa atcggtgcta ttgaccgcgg agcgcgcaac gcaccggatg
     8761 cagtcaaaga tgcggatgtg gtgatcctgg caacaccagt tgatgagttg agggaaacat
     8821 tggaagtgat tgggccactg ctgcgcgaag gcgcggtggt ggtggataca tcagcactca
     8881 aaatcaaagc ggctgaatgg gctaaagaat ttctgcctgc cggtcggcat tttatcggca
     8941 tgatgccaac attgaacccg gcttacctgc acgagaccca aaccgggatt gacgccgccc
     9001 atgcggattt atttcacaac agcctgatgg ttattgcgag ttttccggat acgcaccccg
     9061 atgctgttaa gctggcgtca gacttgagcc agctgctgca cagcaaggcc ttattcaccg
     9121 atccgtatga ggctgatgga ttgacggcag caagtgaact gctgccccaa ctggcggcgg
     9181 ccgcactggt ccacagcatc cagtcccaac cagcctggcg ggaggggcga aaattagcca
     9241 gtcgggccta tgctgaagct acagaaccgg ttctcggcct gaatgagaat gaggcgccgg
     9301 gtgtgacggc attgaataac cgtgagaata cgttgcgggt gcttgatgat ttgattgcca
     9361 cgctcaacga gatgcgggct gtgattgaca gccaggacag cgagcaatta gccaagattc
     9421 tggataaagc gcggcaagac cggcgcgagt ggtgggccca gcgcgtgcgg gctgagtggg
     9481 acaaaattga caatgtgcag accctttcag ccagcgaggc gatcagcggt atttttggct
     9541 ttcgacgcaa ggccaaggaa aagacgcgta accgccgata agtccggagg cagccgacta
     9601 agatggcatt ttattgttat atcttagaat gtgcggatgg gtctttttat accggctgga
     9661 caacagaccc ggtgcgccgg gaaaagcagc ataaccgggg cacgggggca gcatatacgc
     9721 ggacgcatcg tccggtgcgg ctggtttatg ttgagccaca ggccagccgt tctgacgcaa
     9781 tgcgccgcga gcgcgctatc aaacggttaa cacacctcag gaaggaggca ctcatccatg
     9841 gccaatccaa atcgtaacct tctccttgaa caggcaaccc tgactgtgat tgccccaggg
     9901 cgggtgaatt tgctgggcga gcatgtggat tataacgatg ggctggtgct gccagcagcc
     9961 atcaaccgcc atgtgcgcct ggaggccaag ccaagagctg accggcttgt ggtgctgcac
    10021 gctaaagacc tggaagaaac agtctgtttc gaattagatg ggctggagaa gcgccagaca
    10081 agcggcggcg agccgctgcc ccactgggcg ctttacccgg cgggtgtggc ctgggcggca
    10141 cagcagcgcg gcctgcccgt gcaggggatt gaggctgcat acacgtcaga tgtgccgata
    10201 ggagcgggat tgagttcatc ggcagcggtg gaagtggggt ttgcggctct gtggcgatct
    10261 ttgggcggat gggcgttgaa caacctgatg ctggcacaac tcagccagca ggccgaaaat
    10321 cagtatgtgg gggtcaactg cgggttgatg gatcagtttg ccagtgccaa cggggtagaa
    10381 ggacacgggt tgttatttga cacccggtct ttagattttc gccccctgcc gctgcctggc
    10441 gggacggtga ttgtgattgc ggactccggc gtccggcggc ggctggttgg ctcggcttat
    10501 aacgaccggc gggcggcctg cgagcaagct gtggcgctgc ttaaaaaaga gctgccgggc
    10561 atccgggcgc tgcgggatgt gtcgagagat gatttcaacc ggctggctga ccacctgccc
    10621 ccgattgttg cccggcgggc gcggcatatt gtggaagaaa tagaccgcgt ggacgcagct
    10681 attggaatgt tgagccaggg tgatgccgcc ggatttgggc ggttgatgct ggcaacccac
    10741 gcctctttgc gcgacctgta tgaggtcagt attcctgagt tggatgcgct ggttgaaatt
    10801 gctgccagac agcccgggtg ctggggcgca cgactaaccg gcgctggatt tgggggctgc
    10861 acggtgaacc ttgtgcagca agatcgggca gaagccttta tggtggccct caaaaatgac
    10921 tatacgaaaa acaccggcca gcaggcagag gtttatctct gccaagccag ccggggtgtt
    10981 cacgttgagc cgtcagggta agggtcagtc agtaaagacg ggcaggtgac cgagcgaaat
    11041 gatatctgcc cagcgggcgc gttcgccttc agtcagaatt gcagcttcgg caaccacctg
    11101 ggcaccagct ttttgcatca gcaggcgcat gccttgcagt gtggagccgg ttgagatgac
    11161 atcatccaga atgacgaccc gtttgccttc caacacacgc agatcttttt cgtccaggta
    11221 gagcgtctgg ggttgaccgg tggtgatgga cagggtctca gcctggaggg cctgccccat
    11281 gtacggcttg taggccttgc gcaaaatcac atagggcttg cgcgtgacaa ccgagatcgc
    11341 ataggccagt ggaatactct tgctctcagc ggttacgaca acatcataaa ccactgattc
    11401 cattttttcg gccagggctt tggcgcaggc ctgcaccagt tcggtatcgc ccagaatgtt
    11461 gaggatggcg atgcg
//
LOCUS       DAOPVS010000054        11224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_5884_length_11224_cov_55.786552, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000054 DAOPVS010000000
VERSION     DAOPVS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5884_length_11224_cov_55.786552"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKL11_06130"
     CDS             <1..566
                     /locus_tag="PKL11_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ13217.1"
                     /translation="IVPIFKNDAEQAKVMPVVQQLEASLKNFRVKVDARTEVTPGFKF
                     NDWEMRGVPVRIEIGPKDVDKGVVTVARRDVPGREGKITLSPDQIAAPIGELLNEIHT
                     AMLNRARAFRDAHISDPRDYTEFQEAVQNGWALSYWCGDAACEAAIKEDTKATTRCIP
                     AEQPSGPGRCIRCGQPAAEKAYFARAY"
     gene            583..1377
                     /locus_tag="PKL11_06135"
     CDS             583..1377
                     /locus_tag="PKL11_06135"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNZ13218.1"
                     /translation="MPAVQLARLRAQVEELSSRFSQPAVYLRALRDLLELYADRTYQP
                     GQGVPSQVLTPSYHVPMLVMRQLESSLESHARENPIVALPLADLLWQEEYLEPRLLAA
                     SALGQAPLMPPEQILDRLVAWAQTGDERLVRAGLFSHGTDRLRREFPDLWLQRIQVWF
                     SDPNPTAQMMALEALLPLVRDPNYQDLPPIFPLVQPLLRAAPAPLQAPLQDLLEALAR
                     RSSVETTYILRQILTMQRPPALTRLVRRCLPAFDPADQAVLKTLLK"
     gene            1479..1751
                     /gene="rpsO"
                     /locus_tag="PKL11_06140"
     CDS             1479..1751
                     /gene="rpsO"
                     /locus_tag="PKL11_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNZ13219.1"
                     /translation="MTLTKEDKTKLISDHHRHETDTGSPEVQIAILTRRIQQLTEHLR
                     ANKHDESSRRGLLQMVGQRRRLLAYLRRVDYGRYVSLAEKLNLRQR"
     gene            1918..4146
                     /locus_tag="PKL11_06145"
     CDS             1918..4146
                     /locus_tag="PKL11_06145"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNZ13220.1"
                     /translation="MKPTVHQYTTTVGGRPLTFESGKLAAQAGGSVTVRFGDSIVFAA
                     ATMSPNPREAADFFPLTVDYEERLYAGGRIPGSYFRREGRPTEDAILTARLTDRPLRP
                     LFDKNIRNDVQVIMFSMSADGVNPLDVLAINAASAAVMISDIPWAGPVGAVRVGRVNG
                     ELIINPTFSQMAESDLDLRIAGTHDAILMVECGAYEIPEADMVAALMAGHQAIRPIVE
                     AQIKMAAEIGKPKRQVQTFPLDESLVERVSSRITTELTAALDAPHTKAELYTFIDMLQ
                     ATVVAEMASEDATQAPAVKQAFEEAHKRIIRDRILNGTRPDGRTPEEIRPIWCEVDIS
                     PRAHGSGLFTRGETQVLTLATLGTPKEAQELDSLLPQETKRYMHHYNFPPYSTGEVKP
                     LRGQSRREIGHGALAERALVPVIPPESEFPYTLRLVSEVLASNGSSSMGSVCGSTLAL
                     MDSGVPIKAPVSGVAMGLVKEGDRYMILTDIQGMEDHLGDMDFKVAGTTEGITALQMD
                     IKIDGLTADLMSKALAQARTARLSILDQMLAVLPAPRPDLKPHAPRITIIKIPVDKIG
                     AIIGPGGKMIRAIQEETGAKIDIAEDGSVYIATADGVAEARAREMIESLVEVPVLGRI
                     YTGKVVRIADFGAFVEILPGVDGMVHISQLDSERVESVESVVSMGDEISVMVTGIDPA
                     GKIRLSRQAVLEGWTLEEAQASDKPRGGGGGGGRGGGRPQGGGGRRDDRRDNRRGGYD
                     RR"
     gene            complement(4220..5188)
                     /locus_tag="PKL11_06150"
     CDS             complement(4220..5188)
                     /locus_tag="PKL11_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNZ13221.1"
                     /translation="MFFDLPLEQLQQYLPERDEPADFDLFWQTTLEEARAFSLGACFD
                     PVETGLKWMDVFDVTYNGYEGQAIKGWFLLPKERSGPLPCVVEYIGYGGGRGKPIDWL
                     LFANLGYAHFVMDTRGQGSTWQAGDTPDPEPEGANPQYPGFMTRGVLNPKSYYYRRVF
                     TDAVRAVEAARSHPAVDAQRVAVTGGSQGGGITLAVAGLVPDLAAALADVPFLCHYRH
                     ATEIVDTFPYNEISLFLKTHRDKIEVVFNTLAYFDGVNFATRARAPALMCTGLMDDVC
                     PPSTVFAAYNYYAGPKRMKVWPYNHHENGETFQTIEKLNFLKALWG"
     gene            5354..6133
                     /locus_tag="PKL11_06155"
     CDS             5354..6133
                     /locus_tag="PKL11_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13222.1"
                     /translation="MLEKVNLSLALDKAAYKLRLKQAGPWLTLLQRSSWEAGIPIVIV
                     FEGWDASGKGTTINLLTRYMDPRGFHIHPIKAPTDLEKQMPWLWRFWQRLPNYGQVGL
                     FDRSWYGRVMVERVEGFTPRSAWEQAYQDIVDFERTLTDDGYVIIKFFLHISKAEQKK
                     RFKKLEDDPLQSWRVQKEDWEHHRKYKDYVVAIEEMLARTDTEWGPWTIVEATDRCWA
                     RTRVIDTVNHRLEAALQQRNKPVPEMPPLPAEPQPEEVPHA"
     gene            6126..6878
                     /locus_tag="PKL11_06160"
     CDS             6126..6878
                     /locus_tag="PKL11_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13223.1"
                     /translation="MLERVDLTQTLDRADYQQQLEPLQEQLLAYEHLLYERRRCLVVV
                     FEGWDAAGKGGAIKRITEELDSRGYEVYSIAAPAGEDKTHHYLWRFWRRLKPPEEKQI
                     QIFDRSWYGRVLVERVEGFCSEAAWRRAYREINEFERQMVNSGMILAKFWLQISPEEQ
                     LARFEARQGDLAKSWKLTDEDWRNREKWPLYNAAVEEMLLKTSTLPAPWTVVEANDKY
                     FARVKVLQTLVDAAAHGLGVSLSGGERRKKKK"
     gene            6939..8045
                     /locus_tag="PKL11_06165"
     CDS             6939..8045
                     /locus_tag="PKL11_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNZ13224.1"
                     /translation="MTIDRLNRLYSLMSAHSLDAFALNPGPTLTYLTGLEFHLMERPV
                     VLFLSPGTNPIMVLPELEQAKIPQAHIPLQPFPYGENPAQWGSVFAAATRAAGLDGKT
                     IGVEPTLLRFLEFQYVQAAAPQAHLISGAEPLAALRMQKSSAEVARMRRAVQIAENAL
                     AETLRLIRVGMTEREIASELTLQLLRAGSDSAFPFKPIVSGGPNGANPHASPSDRPIQ
                     LGDLLVIDWGAAYEGYISDLTRTFAIGPVDDECRRICEIVKQANAAGRAAGRPGLPAG
                     QVDAAARAVIDQAGYGQYFIHRTGHGIGMEGHEAPYIFGENTLLLAEGMAYTVEPGIY
                     LPDRNGVRIEDNIVVTANGCETLSTLPRELITIG"
     gene            8056..8817
                     /locus_tag="PKL11_06170"
     CDS             8056..8817
                     /locus_tag="PKL11_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ13225.1"
                     /translation="MPTPQLIPTAEPFFYPGGRVGCLLVHGFTGAPTEMHWMGAELAA
                     KGFTVLGIRLAGHSTTISDMLRCRWWDWLASIEDGLSILKGATDQVVVMGLSMGGALS
                     LLSAARYPMSGVIAMSAPYELQHDPRLPYLRWLHWLFPRVSKPTSGWYDPASAEGHIS
                     YAYYPSRQIIELNALLAEVRRSLPEVKVPALLMHSRHDGSVSPDNAPRIMSSLGSTDK
                     ELIWVENSGHMITREPARFVALEAATRFIRRVTSR"
     gene            8814..9950
                     /locus_tag="PKL11_06175"
     CDS             8814..9950
                     /locus_tag="PKL11_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13226.1"
                     /translation="MTRDLILVMISLWAWGTGEGLFIYFQPLYLQQWGADPVLIGAIF
                     GGMGIGMAVSQIPAGYLADRLGPRPIMWLSWGLGAAAALAMAAANTLTAFVIGLLLYG
                     LTAFALAPMNAYITRVRGTLGAGRALTFASAAYNFGAVIGPTVGGAVAEGLGLRTVYW
                     IAAAAFTVSTVLVLFVRNAPPEHHPEPHVKGQLHRNSRFMFLVILSLLTMFSLYLAQP
                     LTPNYLQNERGLSVQQIGWMGSAASLGNVIIMLLLGSLSPLAGFLVGLPLVALFAILM
                     WRGNSLPVFAIGYFLMGGYRLSRSMVLAVGRALIHPAETGLAYGMLETANALATILAP
                     PLAGYLYKSSPALVYQVALLSIGVVLLLNLLVWALLTRRKAAVA"
     gene            9947..10573
                     /locus_tag="PKL11_06180"
     CDS             9947..10573
                     /locus_tag="PKL11_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ13227.1"
                     /translation="MTLNLNVYTLGPLENNTILLADPITRDAVVIDPALGIADILPDL
                     EPWHLTQIWLTHAHFDHFIGIDALQQALGISLPVGLHPDDLPLYQEGGGAAQFNFRLI
                     PGPLPTISFQHGQTLSVGAEKVEVRHVPGHSAGHVLFYLASLGAALVGDLIFAGSVGR
                     TDLPGGSHATLIDSIRTQVLTLPPQTRLVPGHGPETTVVIEAETNPFL"
     gene            10689..11135
                     /locus_tag="PKL11_06185"
     CDS             10689..11135
                     /locus_tag="PKL11_06185"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNZ13228.1"
                     /translation="MPKFEREVEIDATVEAVWQVMTDPTHWPKWFPGIDSIGKVSAVA
                     EGGTFEWIDDGQTGIGTIVKLEPMKRLEIVTQMGKDKDSHVFVLRPTGGFLGLKADEC
                     KVEYTMDPLVGGGILGNFIVGGNPKDTLKVKKAMHLLRKLVESLQG"
BASE COUNT         2262 a         3570 c         2943 g         2449 t
ORIGIN      
        1 tgattgtccc catcttcaaa aacgacgccg agcaggccaa ggttatgccg gttgtacagc
       61 agcttgaagc cagcctcaaa aatttccgcg tcaaagtgga tgcccgcacc gaagtaaccc
      121 ccggcttcaa gttcaatgac tgggaaatgc gcggcgtccc ggtccgcatt gaaattggtc
      181 ccaaagacgt ggacaagggt gttgtcacgg ttgcccgccg tgatgttccc ggccgcgagg
      241 gcaaaatcac cctcagcccc gatcagattg ccgcccccat tggcgaattg ctgaatgaga
      301 tccacaccgc catgctcaac cgtgcccgcg ccttccgcga tgcgcatatt tccgacccgc
      361 gcgactatac tgaattccag gaagccgtcc agaatggctg ggcgctctct tactggtgcg
      421 gcgatgcggc ctgcgaagcc gccatcaaag aagacaccaa ggcaaccacc cgctgtatcc
      481 ccgccgaaca gccatccggc cccgggcgct gcatccgctg cggtcagccc gctgccgaaa
      541 aggcctattt cgcgcgtgcc tattaatccg ggttactgat ttatgcctgc ggtgcagctt
      601 gcgcgcctca gggcgcaggt cgaagaatta agcagtcgct tttctcaacc ggctgtctat
      661 ctccgcgcct tgcgagattt gctggagctt tatgccgacc gcacctacca gcctggacag
      721 ggggtgccct cgcaggtgct cacccccagt tatcatgtcc ctatgctggt catgcgccag
      781 ctcgaatcca gcctggaatc gcacgctcgt gagaacccta tcgtggcact cccgcttgcc
      841 gacttgctct ggcaggaaga atatctggag ccgcgcttgc tggcagcctc ggcccttgga
      901 caggctcctc tcatgccccc cgaacagatc ctggaccgcc tggttgcctg ggcccagacc
      961 ggcgacgagc gcctggtgcg ggccggttta ttctcgcatg gcaccgatcg tctgcgccgt
     1021 gagttccctg atttgtggct gcagcgtatc caggtctggt tttcagaccc caatccaacc
     1081 gctcaaatga tggccctgga ggcgctcctg ccactggtgc gtgatccgaa ctatcaagac
     1141 ctcccgccca tcttcccact ggttcaaccc ttgctccgcg ccgctccggc ccccttacag
     1201 gcccccctgc aagaccttct ggaggcgctt gcccggcgct catcggtgga aaccacttat
     1261 atcctgcgtc aaatcttgac catgcagcgc ccgcctgcac ttacccggct ggttcgccgc
     1321 tgcttgcccg cctttgatcc cgccgaccag gccgtactca aaaccctgct caaataaaga
     1381 cccaaattgt ggtataattc gttccactga ctggacctgt ctgcggcgca agctgcgggc
     1441 atggagacct gcggaaaatc tttttaggag cagatgtcat gactttgaca aaggaagaca
     1501 aaaccaaact tatttctgac caccaccgtc acgagaccga caccggttct cctgaggtgc
     1561 aaatcgccat cctgacccgg cggattcaac agctcactga gcacctgcgc gcgaacaagc
     1621 acgatgaaag ctcgcgccgc ggcctgcttc agatggttgg ccaacgccgc cgccttctgg
     1681 cgtatctgcg ccgtgttgat tacgggcgct acgtctccct ggccgaaaaa ctaaacctcc
     1741 gccagcggta aagcaagccc ccaacgagtg gatggggaag gctccccatc cactcgattt
     1801 ttagtccaac tctattcacg tagtgctgcc ggttgggaat tgaaaagtga gcgcgacctc
     1861 ccgtctcgac cagttttcag tccctgaccg gggcaacaca gagggatcaa acaacttatg
     1921 aaaccaactg tacaccaata cacgaccact gttggtggtc gcccgctcac atttgagtcg
     1981 ggtaaactgg ccgctcaggc gggtggctct gtcaccgtcc gctttggcga ttccatcgtt
     2041 tttgctgctg ccaccatgag ccctaatccg cgcgaagccg ctgatttctt cccgctcacc
     2101 gtagattacg aagagcgcct ctatgctggc ggccgcatcc ccggttccta cttccgccgc
     2161 gaaggccgtc cgaccgaaga cgccatcctc actgcgcgcc tcaccgaccg ccccctgcgc
     2221 ccgctctttg acaaaaacat ccgcaacgat gtccaggtca tcatgttctc gatgtctgcc
     2281 gatggcgtaa acccgctcga tgtcctggcg atcaatgccg cctcggctgc cgtcatgatc
     2341 tcggatatcc cctgggctgg cccggtgggt gctgtccgtg taggccgcgt caatggcgaa
     2401 ttaatcatca accccacctt cagccagatg gccgaatcag atctggacct gcgcattgcc
     2461 ggcacccacg acgccatcct gatggtggag tgcggcgcct acgaaatccc cgaagccgac
     2521 atggtcgccg ccctcatggc cggccatcag gccattcgcc cgattgtcga agcccagatc
     2581 aaaatggctg ccgaaatcgg caaacccaag cgccaggtgc aaaccttccc actggacgaa
     2641 tcactcgtcg agcgtgtctc ttcccgcatc accaccgaac tcaccgccgc cctggatgcc
     2701 ccccacacca aagctgaact ctacaccttc attgacatgc tgcaggcgac cgttgttgcc
     2761 gaaatggcca gcgaggatgc cacccaggcc ccggctgtca agcaggcttt tgaagaagcc
     2821 cacaagcgca tcatccgtga tcgcattctg aatggcaccc gccccgacgg ccgcaccccc
     2881 gaagaaattc gccccatctg gtgcgaggtg gatatttccc ctcgtgccca tggctccggc
     2941 ctcttcaccc gtggcgaaac tcaggtgctc actctggcaa ccctcggtac ccccaaagaa
     3001 gctcaggaac tggactcgct gttgccgcag gaaaccaagc ggtacatgca tcattacaat
     3061 ttcccgccct actccaccgg cgaagtcaag ccgctccgcg gccagtctcg tcgcgagatc
     3121 ggtcacggtg ccctggctga acgcgccctg gtgccggtca tcccccccga atctgaattc
     3181 ccctacaccc tccgcctggt ctctgaggtc ttggcctcca atggctccag ctccatgggt
     3241 tcagtttgtg gctcaaccct tgctctcatg gactcgggtg tgccgatcaa agcccccgtc
     3301 agcggtgtgg cgatgggtct ggtcaaagaa ggcgaccgct acatgatcct gaccgatatc
     3361 cagggcatgg aagatcacct cggcgacatg gacttcaaag ttgccggtac caccgaaggc
     3421 atcactgcct tgcagatgga catcaaaatt gacggcctga ccgccgacct gatgtcaaag
     3481 gctctggccc aggctcgcac cgctcgcctg agcatcctcg accagatgct ggctgtgctc
     3541 ccggcgccgc gtcctgatct caagccgcat gccccgcgca tcaccatcat caagatcccc
     3601 gtggataaaa tcggcgccat catcggccct ggcggcaaga tgatccgcgc tatccaggaa
     3661 gaaactggcg ccaagatcga tatcgccgaa gatggcagcg tctacatcgc taccgctgat
     3721 ggcgttgctg aagctcgcgc tcgcgagatg atcgaaagcc ttgtggaagt gcccgtgctg
     3781 ggccgcatct acaccggcaa ggtcgtccgc atcgccgact ttggcgcttt tgtcgaaatt
     3841 ctccccggcg tagatggcat ggttcacatc tcccagcttg acagcgaacg tgttgaaagt
     3901 gttgaatcgg ttgtctcgat gggcgatgaa atctccgtca tggtcaccgg catcgacccc
     3961 gccggcaaga tccgcctgtc tcgccaggct gtcctcgaag gctggacgct tgaagaagcc
     4021 caggccagcg acaagccgcg tggcggcggt ggtggcggcg gccgcggtgg cggacgcccg
     4081 cagggtggtg gtggccggcg cgatgatcgc cgtgacaacc gccgcggtgg ttacgatcgc
     4141 cgctaatccc aattgacgaa ttataaaagc tgcgcctgca aaggcgcagc tttttgattg
     4201 cccattccaa tcagcaggtt cagccccaca gagccttcaa aaaattaagc ttttcaatcg
     4261 tttgaaaggt ttcgccgttt tcgtggtggt tgtaaggcca cactttcatc cgttttggcc
     4321 ccgcataata attgtaagct gcaaaaaccg tagatggcgg gcagacatca tccatcagtc
     4381 cggtgcacat caaagctgga gcacgggcgc gggtggcaaa attgaccccg tcaaagtagg
     4441 ccagcgtgtt gaatacaacc tcaatcttgt ctcggtgagt cttcagaaat aacgaaattt
     4501 cattataagg aaaggtatcc acaatttctg tggcgtgccg gtaatggcac aaaaacggca
     4561 catctgccag ggccgccgcc aggtcaggca ccagaccggc aaccgccagg gtgatgccgc
     4621 cgccctgact gcccccggtc actgccacgc gttgtgcatc caccgccgga tggcttctgg
     4681 cagcttctac agcccgaacc gcatccgtga acacccgccg ataataatag cttttggggt
     4741 tgagcactcc ccgggtcata aaaccgggat attgggggtt ggcaccttct ggctccgggt
     4801 ctggtgtgtc gccagcctgc caggtgctgc cctgcccgcg ggtatccatc acaaaatgag
     4861 catatcccag attggcaaac agcagccagt caatgggttt gccgcgcccg ccgccatagc
     4921 caatgtactc caccacgcaa ggcagtggcc ccgaccgctc tttgggcagc aggaaccatc
     4981 ctttaattgc ctggccctcg taaccgttat aagtcacatc aaaaacatcc atccacttca
     5041 aacccgtctc aaccggatca aagcaggcgc ctaatgaaaa tgcccgggct tcttccagcg
     5101 ttgtttgcca aaacaggtca aaatcggctg gctcatcccg ctctggaaga tattgctgca
     5161 gttgttctaa aggcaagtca aaaaacatag catgctcctt gccaataagt ataccgctct
     5221 ccagaaaacc atgtgagcag cgctgaaaca ttcccggccc gccattctat tcgtttctcc
     5281 gcttgccttt tccggtttct gtgctacagt ttaatcatca tccaggcaat tgttcttctt
     5341 gggaggacgc attatgcttg aaaaagtcaa tctttccctg gcgctggaca aagccgccta
     5401 caaactgcgc ctcaaacagg ctggcccctg gctcacgctc ttacagcgtt cttcttggga
     5461 ggctggcatt cctatcgtca ttgtttttga aggctgggat gcctctggca aaggcaccac
     5521 catcaacctg ctcacccgtt acatggaccc gcgtggcttc catatccatc cgattaaggc
     5581 ccccaccgac ctggaaaaac aaatgccctg gctctggcgt ttctggcagc gcctgcccaa
     5641 ttatgggcag gtcggcctct ttgatcgcag ttggtatgga cgcgtcatgg tcgaacgggt
     5701 tgagggcttt accccgcgtt ccgcctggga acaggcctat caagacattg ttgattttga
     5761 acgtaccttg accgatgacg gttacgtcat tatcaaattc ttcctgcaca tcagcaaagc
     5821 cgaacagaaa aaacgcttca aaaaattgga agacgacccg ctccagtcct ggcgggtgca
     5881 aaaagaagac tgggaacacc accgcaaata caaagactat gttgttgcca ttgaagaaat
     5941 gctggcccgc accgataccg aatggggccc ctggactatc gttgaagcga ccgatcgttg
     6001 ctgggcgcgc acccgggtaa ttgatacggt caatcaccgc ctcgaagccg ccttgcagca
     6061 gcgtaataaa ccggtccctg aaatgccccc cttacccgcc gaacctcaac ccgaggaggt
     6121 gccgcatgct tgaacgcgta gatctgaccc agacgctcga tcgtgccgat tatcagcagc
     6181 agcttgaacc cttgcaggag cagttgttgg cttacgaaca cctgctctat gagcgccgcc
     6241 gctgcctggt ggtcgtcttt gaaggctggg acgctgccgg caagggcggc gccatcaaac
     6301 gcatcactga agaactggac tctcgcggct atgaggtcta ttccattgcg gccccagccg
     6361 gcgaagataa aacccatcat tatctctggc gtttctggcg gcggctcaaa ccgcccgaag
     6421 agaaacagat ccagatcttt gaccgcagct ggtatggtcg tgttttggtt gaacgcgtgg
     6481 aaggcttctg ctccgaagct gcctggcgcc gggcttaccg tgagattaac gaatttgaac
     6541 gccagatggt caattccggc atgatcctgg cgaaattctg gctacagatt agccccgagg
     6601 aacaattggc tcggtttgaa gctcggcagg gcgacctcgc caaatcctgg aagctgacag
     6661 acgaagactg gcgcaaccgt gaaaaatggc cgctctacaa tgccgccgtt gaagagatgc
     6721 tgctcaaaac ctcaaccctg cccgccccct ggacggttgt cgaggccaac gacaagtact
     6781 ttgcccgcgt caaagtcctt caaaccctcg ttgatgccgc cgcccacggt ctgggcgttt
     6841 ctctgtccgg tggcgagcgc cgtaaaaaga aaaaataaaa ccggtcgggt gccccctcac
     6901 catccggatt agattataat caaattggag gtgtccccat gaccattgat cgcctgaatc
     6961 ggctttattc cctcatgtct gctcacagcc tggacgcatt cgccctcaat cctggcccaa
     7021 cgctgaccta cctgacgggc cttgaattcc atttgatgga gcgcccggtt gtcttattcc
     7081 tatcaccggg gaccaacccc atcatggtcc tgcctgaatt ggagcaggcc aagatccccc
     7141 aggcgcatat tcccctgcag cccttccctt atggcgaaaa cccggcccaa tggggcagcg
     7201 tttttgccgc cgccacccgt gcggctggtc tggatggtaa aaccatcggc gttgagccca
     7261 ccctgctgcg gttcctggaa tttcaatatg tccaggccgc cgccccgcag gctcacttga
     7321 tttctggcgc cgagcctctg gctgccctgc ggatgcaaaa atcatccgcc gaggttgccc
     7381 gtatgcgccg ggctgtccag atcgccgaaa atgccctcgc cgagaccctg cgtctcattc
     7441 gcgtcggcat gaccgagcgc gaaattgcca gcgaactgac cctgcagttg ctccgcgccg
     7501 gctccgattc agccttcccc ttcaagccca ttgtttccgg cggccccaat ggcgccaatc
     7561 cccatgcctc acccagtgac cgccccatcc aactcggcga cctgctggtg attgattggg
     7621 gcgctgccta cgaggggtac atctccgacc tcacccggac gtttgccatt ggccccgtag
     7681 atgatgagtg ccgccgcatc tgtgagatcg tcaagcaggc caacgcggct ggccgtgcgg
     7741 ccggtcgccc cggcctccct gccggccagg tcgatgctgc cgcccgcgct gtgattgacc
     7801 aggccggtta cggccagtat tttattcacc gcaccgggca cggcattggc atggagggcc
     7861 acgaagctcc gtatatcttt ggcgagaaca cgctcttgct ggctgaaggc atggcctata
     7921 cggttgaacc gggcatctac ctgccagacc gcaacggtgt ccgcattgaa gataacattg
     7981 tcgtgacagc caatggctgt gaaacgcttt caacgctgcc gcgcgaactc atcaccattg
     8041 gataggtacc gccacatgcc aaccccacaa ctcattccaa cagccgaacc cttcttttac
     8101 cccggcggac gggtgggctg cctgctggta catggcttca ccggcgcccc caccgaaatg
     8161 cactggatgg gcgctgaact ggccgccaaa ggatttaccg ttcttggcat ccgtcttgcc
     8221 ggccacagca ccaccatttc agatatgctc cgctgccgtt ggtgggattg gctggcatct
     8281 attgaggatg gtctatccat tctcaaaggc gccaccgatc aggtggtggt catgggcctt
     8341 tccatgggcg gcgcactctc cctgctcagc gcggcccgct atcccatgtc cggcgtgatt
     8401 gccatgtctg ccccctatga actccagcac gacccgcgcc tgccgtacct gcgctggctg
     8461 cactggcttt ttccccgggt atccaagccc accagcggct ggtatgaccc ggcttctgct
     8521 gaagggcata tcagctatgc ctattacccc tcccgccaga ttattgagct gaacgctctt
     8581 cttgctgagg ttcgccgttc gctgccagag gtcaaggtgc ccgccctgct catgcattcc
     8641 cgccacgatg gcagtgtttc accggataac gcccccagga tcatgtccag cctgggatca
     8701 acggataaag agctcatctg ggttgaaaac agcggtcata tgatcacccg cgaaccggcg
     8761 cgctttgtcg ctctcgaagc cgccacgcgc tttattcgcc gggtgacctc aagatgaccc
     8821 gtgacctgat tctggtgatg atctccctct gggcctgggg caccggagaa ggtctcttca
     8881 tttactttca accgctttac ctccagcagt ggggtgctga ccctgttttg attggcgcca
     8941 tctttggcgg catgggcatc ggcatggcgg tttctcaaat cccggcgggt tacctggccg
     9001 atcgcctggg cccccgtccg attatgtggc tttcctgggg actgggggct gctgctgctc
     9061 tggccatggc cgccgccaac accctgaccg cctttgtgat tggactgctg ctctatggtc
     9121 tcaccgcctt tgccctggcc cccatgaacg cctacatcac gcgggtgcgg ggcacattgg
     9181 gtgcagggcg cgccctgaca tttgcttctg ccgcttataa ctttggcgct gtaattggcc
     9241 caactgtagg aggcgccgtt gctgaaggat tgggattgcg cacggtctat tggattgcag
     9301 cggcagcctt taccgtctct actgtactgg ttttgtttgt gcgcaacgcc ccgccagagc
     9361 atcatcccga gccgcacgtt aaaggtcaac tgcatcgcaa ttcccgtttt atgtttctgg
     9421 tcattctgtc gctgttgacc atgttctccc tctacctggc ccagccgctc acgcccaatt
     9481 acctccaaaa tgagcgcggc ctctcggtcc agcaaatcgg ctggatgggc tctgctgcca
     9541 gtctgggcaa cgtgatcatt atgctgctgc tcggcagcct atctccactg gcgggctttc
     9601 tcgttggact gcctctggtg gctttatttg ctatcttaat gtggcgggga aattccctcc
     9661 cggtttttgc aatcggctac tttttgatgg gcggttaccg cctcagtcgc tcaatggtat
     9721 tggcagttgg ccgggccctc attcaccccg ctgaaaccgg tctggcatat ggcatgcttg
     9781 agactgcaaa tgcgcttgcc accatcctgg ccccacccct tgccgggtat ctctacaaaa
     9841 gtagccccgc gctggtttat caggtcgccc tgctgtctat tggcgttgta ctactcctga
     9901 acctgttggt ttgggcgctg ctgaccagac gaaaggccgc tgttgcatga ccctgaatct
     9961 taatgtctat accctgggcc cgctcgaaaa taacaccatc ttgctggccg atcccatcac
    10021 ccgcgacgct gtggtgattg accccgccct gggcattgcc gatatcctgc ccgacctgga
    10081 gccctggcac ctgacccaga tctggctcac ccacgcccac tttgatcatt tcatcggcat
    10141 cgatgcgctt cagcaggccc ttggcatcag tttgccggtt ggcctgcatc cggacgatct
    10201 gccgctgtat caggaggggg gcggcgccgc ccagttcaac ttccggttga ttcccggccc
    10261 cctgccaacg atcagttttc agcatgggca aaccttatct gttggcgccg aaaaagtcga
    10321 ggtccggcat gtccccggcc attctgccgg gcatgtcctg ttttaccttg ccagcctggg
    10381 cgccgctttg gtcggcgacc tcatctttgc tggcagcgtt gggcgcactg acctgcctgg
    10441 cggcagtcac gctaccctga ttgacagcat ccgcacccag gtgctcaccc tgccgcccca
    10501 aacccgtctc gtccccggtc acggcccaga aaccaccgtc gtcattgaag ctgaaaccaa
    10561 tcccttcctc tgagttgatc gtgtctcaaa cttcaacaaa tttgacattc tcattaatca
    10621 gactataatg atttaaatca ttgcagtttt tggctgtaat ggaaatccca aaggaggtaa
    10681 tcagtaaaat gcctaagttc gaacgtgaag ttgaaattga tgcgaccgtc gaagccgttt
    10741 ggcaggttat gacggaccca acgcattggc ccaagtggtt ccctggaatc gacagcattg
    10801 gaaaagtatc cgctgttgct gagggcggta cctttgagtg gattgatgac ggccagaccg
    10861 gcatcggcac catcgtgaaa ctggaaccca tgaagcgtct tgaaatcgtc acccagatgg
    10921 gcaaagacaa agactcgcac gtcttcgtcc tgcgccctac tggtggtttc ctgggcctca
    10981 aggctgacga atgtaaagtg gaatatacca tggacccact ggttggcggc ggcatcctgg
    11041 gtaactttat cgtcggcggc aaccccaaag acaccctcaa ggtcaagaaa gccatgcatt
    11101 tgctgcggaa actggtcgag agcctgcaag gctgagtcca ttccgccgtt gatttctaaa
    11161 gaataggatt aggagataca ccatgaacct ttttgcaaca attgtcgttg gcctgattgc
    11221 cggt
//
LOCUS       DAOPVS010000055        11079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_5968_length_11079_cov_61.749365, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000055 DAOPVS010000000
VERSION     DAOPVS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5968_length_11079_cov_61.749365"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..857
                     /locus_tag="PKL11_06190"
     CDS             180..857
                     /locus_tag="PKL11_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13229.1"
                     /translation="MPNRAKILLVEGKRSDRPSLVTGLTKKGFNVETASNGSNAQERL
                     KDAHAHLIIVDAASMRTSGKRIVTALRLQAPTLPLVLIVDEQADPNEKYGADVLLVAP
                     FTLQKLLNRIKPLLPAEQKDTLQVGPLELDVRHRWVRCHDRQARLTPRLVALLKTLME
                     RVGQVIPREELFTQVWETNYAGDMRTLDVHISWLRQAIEEDPRRPHYLKTLRGMGYRL
                     DIDDNDG"
     gene            847..2361
                     /locus_tag="PKL11_06195"
     CDS             847..2361
                     /locus_tag="PKL11_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13230.1"
                     /translation="MTDSRPDKLQPPAHHAPALLPRLEAAVRVLFFPGFVLFVLWVLL
                     GNSIEIDSWWHLAHGRWMVENGQLLTTDPFSYTRPGAIWSHPGAWYEILLYQMYTWLG
                     YIGVDLTAGVVLAAPFCVLWAYLKTPHLRRFALVLSGLVLSAVYWSARPNIFTLLFTL
                     LTLILLDQHQRGSRWALWLLPVVMVAWVNTHGGWPVGFLLTGAFALDEIRWPRRLNRA
                     ALRDQNYRRLGQFALVLALMAAATFINPYGWTMHREMFLTTTRVAEDTMIQEWQSPNF
                     HRPYGQVFLAAILISAVIVVRRRGPGQVGRLAALAGVLTMGLVSARHIAISAVVLPLF
                     WADLLGWRVPAGEKLAVKSPAPAVTGVALAFFCFSILMFGGRLDTVLHTPRDLSAWPT
                     GAVEYLRTARPEGHIFNNYQMGGYLIWALPEYPVFLDSRSDIYGDEIIIQSDRVERAL
                     PGWEAILKQWDIRIALTTADSALSSALAQKPEWELCQQDAASVLWIQTGGQGCP"
     gene            complement(2395..3057)
                     /locus_tag="PKL11_06200"
     CDS             complement(2395..3057)
                     /locus_tag="PKL11_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ13231.1"
                     /translation="MKRDTLQKILRFLIRVLTDTRFEGVEHIPTQGGVILATNHLSRL
                     DIPVLFVNPVRNDLTGLVADKYKRHSIIAWFTRQAQGIWLDRTKADFSAFRDAIAALG
                     EQRVLGIAPEGTRSPGQLLEGKPGTILIATRANVPIVPVAITGTKGGMQKILTFRRPH
                     IRVRFGPAFQVPALSRENREAELKYWTDELMCRIAVMLPESYRGVYADHPRLAELIAA
                     EQ"
     gene            complement(3069..3746)
                     /locus_tag="PKL11_06205"
     CDS             complement(3069..3746)
                     /locus_tag="PKL11_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNZ13232.1"
                     /translation="MDFIMQIVDFILHVDKYLDMIIAAVGNWTYVVLFIIIFIETGLV
                     VTPFLPGDSLLFAAGAFAARGAFDVILLYVLMAFAAVLGDTANYWIGHYIGPRVFTTK
                     SRLFKKEYLDRTQAFYEKHGGKTIFLARFIPIIRTFAPFVAGVGKMTYGHFITYNIVG
                     GIVWTAVFIFGGYFFGNLPFVEKNFSLVIIAIIVISVLPAVYEAIKNRRQPAKPAAEE
                     KTPAATE"
     gene            complement(3771..4991)
                     /locus_tag="PKL11_06210"
     CDS             complement(3771..4991)
                     /locus_tag="PKL11_06210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNZ13233.1"
                     /translation="MASRAGFHYPTLAQLILSACGGLFGLFAAALLLLMGGMNLVSGN
                     TTSQTTSLLNLAGGTALVGLLCLPSAVYAMLRLLERPLPDWRRMPGIRLFAIGLPVLW
                     GMALVLAAVVIGHPAETFVIPIIYPVVVGVPIAWLLLIAGRGLLLGGPQRRWGTVSLS
                     LTGTLITVLVTEVLVLGVLILIFAFWLAANPDQMAFFQNFAAVVETVEAQDSMDVMLR
                     QLFQYPGVIPGLITLAAVIIPLMEELIKPLALYFLLGRRLTPAQGFVGGLICGACFAL
                     TESIGSLSQVADSSSWIMLAVGRGGTALLHITCTGLVGWGVVSAFTERRWGRFFLAFL
                     GAVALHGTWNAATIAQSVSTLMDGALWKTLGALSPVMLGIIAMSNLIILLVMNRRLQP
                     PVEVPAVAPAGMDA"
     gene            complement(4991..6265)
                     /locus_tag="PKL11_06215"
     CDS             complement(4991..6265)
                     /locus_tag="PKL11_06215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ13234.1"
                     /translation="MKKFVFPLLVMLSLLLAACDGMIQAEPTAIPTPVPATATPVVQE
                     GATVPDCQVASIFPEPRAAYEITIPLPEAEDHIRGAKDGRMTLIVYSDFQCPYCAQLE
                     PQIKAFLDAYPEEVTVIFRNLPLISIHEKAQLAAQAAEAAALQDKFWEMHDALVDANN
                     WSAWTALSLEEFPSWLKTTATSIGLDSEKFMTDLTSKAIEESVSAETQANYALGLNST
                     PSVFLFMDGNLYFTPEDQIPFTYANLELIRSLYDLRAIEYKECPANTLKDNKTYTATL
                     KTTKGDIVIELYAKEAPLTVNSFIFLSKEGWYDNVPFHRVLDGFVAQAGDPTGTGVGG
                     PGYMIKDEIVDTLKYDQAGVVGMANSGANTNGSQFFITLDAQPNLDGSYTIFGHVVEG
                     MDVVNALTKRDPSSSTDLPEPDYILSVTITEK"
     gene            complement(6320..6943)
                     /gene="tmk"
                     /locus_tag="PKL11_06220"
     CDS             complement(6320..6943)
                     /gene="tmk"
                     /locus_tag="PKL11_06220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNZ13235.1"
                     /translation="MFITLEGPEGSGKTSQLPRLADYLREQGYDVLTTREPGGTIIGD
                     QVRAVLTRLDNTAMQPRTEILLFLAARAQLVEEVIRPALAQGKIILSDRYADSTLAYQ
                     GYGHGVDLALLRLLLDFATGGLRPDLTLLLDVDPAVGLSRRRSSGGEWNRLDAYALEF
                     HQRVRQGYLTMAHQEPTRWRVVDASQPPDVVQSALSQLVLNRLRCDV"
     gene            complement(6947..8563)
                     /locus_tag="PKL11_06225"
     CDS             complement(6947..8563)
                     /locus_tag="PKL11_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNZ13236.1"
                     /translation="MTQRRITDDLHTLLGVLPPAIAQAVTRANHEDALIEIVLDLGRL
                     PKARFLNEEVTLSDVEVTSAEIETVVARIGDFDADNRAGLERTLHRISAIRNRRGHIV
                     GLTCRVGRAVYGTIDIIQDLVESGQSILLLGRPGIGKTTMLREAARILAETKRVIIVD
                     TSNEIGGDGDVPHPAVGRARRMQVAAPSLQHEVMIEAVENHNPEVIVIDEIGRELEAQ
                     AARTIAERGVQLVATAHGRTLENLLLNPTLSDLIGGIEAVTLSDEEARRRGTQKTVLE
                     RRSPPTFDVLIEIQERDQMAVHPDVSAAVDALVRGAPLPPEIRSRGADGEIKVEKPAP
                     PPPASRALNQGSRRAYANGLEEEPYAPSRPARSPRPEYEPAARPGREPLNPVRVYAYG
                     VARNRLTQAARRVGVPAIVVQDIAEADVLMTLRSYYRSHEQTIVDAEQRGMPIYVLRA
                     NTVSQMEQSLAELFNLAATPDNGPAEWDFVSAQTAAAIQAVLNGQKWIDLPPASPQVR
                     RMQHEMARQAQLVSHSYGKEPHRRVRIFRE"
     gene            complement(8538..9428)
                     /locus_tag="PKL11_06230"
     CDS             complement(8538..9428)
                     /locus_tag="PKL11_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13237.1"
                     /translation="MTHGSPIGLGLLLRHLRLGDYGDSAAVVELGRSRLLGRMRYTLG
                     QRAAHLTFLMPQDALESTPGAVLLDSLAAAAGERGALCLLAESADDLGTFELLRRSGF
                     AVYAWQRYWRVAAAPQAAADLSWRPYRAADENAVRSLHQQLTPPLVQAAEAFEPRFAS
                     GWVHWQSGALRAYVDVQSGPRGVCLQPLIHPEVSDAGALLDALLAYPDRWLGRPVYLA
                     VRSYQAWLEPLLEARGVPASTRHSLMVRHLVVFSRSLQPATAQAALEKGWAGPTPTAH
                     TINGNKRVQSELYDTETHNR"
     gene            complement(9512..10117)
                     /gene="pdxT"
                     /locus_tag="PKL11_06235"
     CDS             complement(9512..10117)
                     /gene="pdxT"
                     /locus_tag="PKL11_06235"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNZ13238.1"
                     /translation="MALQIGVLALQGAFAEHQVVLKRLGASVTQVRLADELAGLDGLI
                     IPGGESTTMGKLAVDFGLLEPLRTFGQTHAIWGTCAGAILLSRDARRAQPLLGLMDIT
                     VERNAFGRQTESFEADLTINALPEPERPFHAVFIRAPLIESIGPGVQPLAALPGDFAG
                     RRPGLGGKIVAAQQGRLLATAFHPELTADDRFHRYFMELAA"
     gene            complement(10118..11005)
                     /gene="pdxS"
                     /locus_tag="PKL11_06240"
     CDS             complement(10118..11005)
                     /gene="pdxS"
                     /locus_tag="PKL11_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNZ13239.1"
                     /translation="MEQQVGTFAVKQGLAQMLKGGVIMDVVTPEQARIAEEAGACAVM
                     ALERVPADIRAQGGVARMSDPELILKIMDSVSIPVMAKCRIGHFVEAQILQSLGVDYI
                     DESEVLTPADEAFHIYKHAFNVPFVCGCRNLGEALRRIGEGAAMMRTKGEAGTGDVVE
                     AVRHARQVLGDIRRLQTMPEEELMTYAKELGAPYELLVQTRSLGRLPVVNFAAGGIAT
                     PADAALMMQLGVDGVFVGSGIFKSGNPATRAAAIVKAVTHYTDAAILAEVSRNLGEPM
                     VGRTVANLPDAERMAGRGW"
BASE COUNT         2263 a         3123 c         3580 g         2113 t
ORIGIN      
        1 gcgttcttta agtttttaag gtataatcat gtaacgtcgg ggtgtatgtt ccctggcgga
       61 tgatgggcag cggttcggct ggcacgccag cagacaccaa gacaaccgcc ggggcaatgc
      121 gcagaccact gcccaagcca gaacagcacg ttcaaccttc gcacatcagg ctggtgctta
      181 tgccaaatcg ggccaaaatc ctacttgtgg aaggcaagcg ctcagaccgt ccctcactgg
      241 tgacggggct gacaaaaaaa ggttttaacg ttgagaccgc ctccaacggc agcaatgcgc
      301 aggagcggct taaagacgcg catgcccatc tgattattgt ggatgcggct tcaatgcgca
      361 ccagcggcaa acggattgtt acggcgctgc gcctgcaggc ccccaccctg ccgctggtct
      421 tgattgtgga tgaacaggca gaccccaatg aaaaatatgg cgccgatgtt ctgcttgtgg
      481 cgccatttac actgcaaaag ctgctgaacc gcatcaagcc gctgctgcct gccgagcaga
      541 aagatactct gcaagttggc ccgctggagc tggatgtgcg gcatcgctgg gtgcgctgcc
      601 atgaccgcca ggcccggctg acgccccggc tggtagcgct gctcaaaacc ctgatggagc
      661 gcgtggggca ggttattccc cgggaagaac tctttaccca ggtgtgggaa accaattacg
      721 ccggcgatat gcgcaccctg gatgtccaca ttagctggct caggcaggcc attgaagaag
      781 acccgcgccg cccgcactac ctgaagaccc tgcgcgggat gggttaccgc ctggatattg
      841 acgacaatga cggatagccg tccagacaaa ttacagccac ctgcacacca cgcgcccgct
      901 ttgctgcccc gactggaggc ggcagtgcgg gtgctgtttt tcccaggctt tgtgctgttt
      961 gtgttgtggg tgctgctggg caactccatt gaaattgata gttggtggca cctggcgcat
     1021 gggcggtgga tggttgagaa cgggcagctg ctgaccaccg acccgttttc ttacacccgc
     1081 ccgggggcca tctggtcgca cccgggggcc tggtatgaga ttttgctcta ccagatgtac
     1141 acctggttgg gctatatcgg cgtggatctg accgccggag tggtgctggc ggcaccgttt
     1201 tgtgttttgt gggcctactt gaagacaccc cacctgcgcc ggtttgcact ggtgttgagc
     1261 gggctggtgc tttctgccgt ctactggtcg gcgcggccca atatcttcac actgctgttt
     1321 acgctgttga ccctgatttt gctggaccag catcagcgcg gcagccgctg ggcgctgtgg
     1381 ctgctgccgg tggtgatggt ggcctgggtg aatactcacg gcggctggcc ggtgggattt
     1441 ttactgaccg gtgcctttgc tttagatgaa atccgctggc cccggcggct gaaccgggcc
     1501 gccttgcgcg atcagaacta ccggcgattg gggcagtttg ccctggtgct ggccttgatg
     1561 gcggcggcga cattcatcaa cccctacggc tggaccatgc accgggaaat gtttttgacc
     1621 accacacggg tggccgaaga cacaatgatt caagagtggc agtcgcctaa ttttcaccgc
     1681 ccctacgggc aagttttcct ggcggcaatt ttgatcagcg cggtgattgt ggtgcggcgg
     1741 cgcggccccg ggcaggtggg gcggctggca gcattagccg gggtactgac tatggggctg
     1801 gtttcggcgc ggcacattgc catcagcgcg gtggtgctgc cgctgttctg ggccgacctg
     1861 ttaggttggc gcgttccggc aggcgaaaag ctggcggtca aatctccggc gccggcggtg
     1921 accggtgtgg cgctggcctt cttctgcttt tcgatcctta tgtttggggg gcgtttggat
     1981 accgtgctgc acacaccgcg cgatctttct gcctggccaa ctggcgcggt tgaatatctc
     2041 agaacggctc gcccggaagg acatattttt aataactatc aaatgggcgg atacttgatc
     2101 tgggcgctgc ccgaataccc ggtctttctg gacagccggt ctgatattta tggcgatgaa
     2161 atcatcatcc aatcggaccg ggtggagcgg gcactgcccg gctgggaggc cattttgaag
     2221 caatgggata tccgcattgc cctgaccacg gctgattcgg cgttgagcag tgcattagcc
     2281 caaaagcccg agtgggaatt atgccagcag gacgcggcca gcgtcctgtg gattcaaacc
     2341 ggggggcaag gctgccccta aacgccccct gttagcccga aaaaaagccg ccgattactg
     2401 ctcggcggcg atcaattctg ccagacgggg atggtcagca tagacgccgc ggtagctttc
     2461 gggcagcatg acagcgatgc ggcacatcag ttcatctgtc cagtatttca gttcagcttc
     2521 gcggttttca cggctgaggg ccggcacctg gaaggccggg ccaaagcgca cccggatatg
     2581 ggggcggcgg aaggtcagga tcttctgcat gccgcccttg gtaccggtaa tggcaaccgg
     2641 cacaatcggc acgttggcgc gcgtggcaat caagatggtg ccgggcttgc cctccagcaa
     2701 ttgccccggg ctgcgggtgc cttcgggggc aatgcccagg actctctgtt cacccagtgc
     2761 agcgatggca tcgcgaaagg ccgagaaatc cgccttggtg cggtccagcc aaatgccctg
     2821 ggcctgacgg gtgaaccagg cgatgatgga gtggcgctta tatttgtctg ccaccaaccc
     2881 ggtgaggtcg ttgcgcaccg ggttgacaaa cagcaccggg atatccaatc ggctgaggtg
     2941 attggtcgcc agaatcacac cgccctgggt gggaatgtgt tccacgcctt caaagcgcgt
     3001 gtcggtaaga acgcgaatca aaaagcggag aattttctgg agggtatcac gtttcatagg
     3061 atcaagtatt actccgtggc ggcaggagtt ttttcttcgg cagcgggttt ggcgggctgg
     3121 cggcgattct tgatcgcctc atagactgct ggcagcaccg aaatgacgat gatggcgata
     3181 atgaccagcg agaagttctt ttcgacgaat ggcaggttgc caaagaagta gccgccaaag
     3241 ataaatacgg ccgtccaaac aatgccgcca acaatattat acgtgataaa gtgtccgtag
     3301 gtcatcttgc caacgccagc aacaaagggc gcaaaggtgc gaatgatggg gatgaagcgg
     3361 gccaggaaga tggtcttgcc gccgtgtttt tcataaaatg cctgggtgcg gtcaaggtat
     3421 tctttcttga acaggcggga tttggtggtg aagacgcgcg ggccaatgta gtgcccgatc
     3481 cagtaattgg cggtatcgcc gagcacggca gcgaatgcca tcaagacgta aagcagaatg
     3541 acgtcaaagg cgccgcgggc cgcaaaggca ccagcagcaa agagcagcga atcaccgggc
     3601 aggaaggggg tgaccacaag cccggtttcg atgaagatga tgatgaacag aaccacataa
     3661 gtccaattgc cgactgcggc aataatcatg tcaagatatt tgtccacgtg gaggatgaag
     3721 tccacgattt gcataataaa atccatatgt tttcctcttg attgatttaa ttaagcatcc
     3781 atgccagcgg gcgcaactgc gggcacctca accggcggct gaaggcggcg gttcatcacc
     3841 agcaggatga tcagattgct catggcgatg atgcccagca tcaccggtga cagggcgccg
     3901 agcgttttcc agagcgcgcc gtccatgagc gttgaaacgc tctgggcaat ggtggccgcg
     3961 ttccaggtgc catgcagcgc taccgcgccc agaaaagcca ggaagaaccg gccccagcgg
     4021 cgttcggtga aggcagaaac caccccccag ccaaccaggc cggtgcaggt gatgtgcagc
     4081 agtgcggtgc cgccgcgccc aactgccagc atgatccaac tggaagagtc ggccacctgg
     4141 ctcagtgagc cgatgctttc ggtcagggca aagcaggcgc cgcagatcag gccgcccaca
     4201 aatccctggg cgggggtcag gcggcggccc agcaaaaagt acagcgccag gggcttaatc
     4261 agctcttcca tcagggggat aatcacggct gccagggtga tgaggccggg gattacgccg
     4321 ggatactgga aaagctggcg cagcataaca tccatagaat cttgcgcctc aacggtctca
     4381 accacggcag caaaattctg aaaaaaggcc atttggtccg ggttagctgc cagccagaag
     4441 gcaaatatca aaatcagcac tcccagcacc agtacctcgg tcaccagtac ggtgatcagg
     4501 gtgcccgtca ggctcaggct gacggttccc cagcggcgct gagggccgcc cagcagcaag
     4561 ccgcgccctg cgatgagcag cagccaggca atgggcaccc ccaccactac ggggtagatg
     4621 atgggaatga caaaggtttc ggcggggtga ccaatgacca ccgcggcgag caccagcgcc
     4681 atgccccaga gtacgggcag gccaatggca aacaggcgaa tgccgggcat ccgccgccag
     4741 tcgggcaggg gacgctccag caggcgcagc atggcataga ctgccgaagg caggcagagc
     4801 aagccaacca gcgccgtgcc gccagccagg ttaagcaaac tggttgtctg ggatgtggta
     4861 ttccccgaga ccaggttcat gccccccatc aagagcaaca gcgcggcggc gaacaggccg
     4921 aacagtccgc cgcacgcgct tagaatgagc tgggcgagcg tagggtaatg aaatcccgcg
     4981 cgcgacgcca ttatttctca gtgatggtca ctgacaagat gtaatcgggt tccggcagat
     5041 cagtgctgga agacggatca cgcttggtca gtgcgttgac cacatccata ccctcaacca
     5101 catggccgaa gatggtgtaa ctgccatcca gattcggctg ggcatccagg gtgatgaaga
     5161 actggctacc gttggtattg gcgcccgaat tggccatgcc aaccacacca gcctgatcat
     5221 acttgagggt atcgacaatt tcgtccttga tcatgtagcc ggggccgccc acaccggtac
     5281 cggtgggatc gccagcctgg gccacaaagc catccagcac acggtggaag ggcacattgt
     5341 cgtaccagcc ctctttcgag aggaagatga agctgttgac cgtgagcggg gcttccttgg
     5401 catacagctc gatcacaatg tcgcctttgg tggttttgag ggtggcggtg taggtcttgt
     5461 tgtctttgag tgtgttggcc gggcattctt tgtattcaat ggcgcgcagg tcatacagac
     5521 tgcggatcag ttcgaggttg gcataggtga agggaatctg atcttcgggc gtgaagtaca
     5581 ggttgccgtc catgaagagg aaaaccgaag gggtggagtt gaggcccagg gcatagttgg
     5641 cctgggtttc agccgagacg gattcctcaa tggctttgct ggtgagatcg gtcataaatt
     5701 tttccgaatc caggccgatg gaggtggcgg tggtcttcag ccagctgggg aactcttcca
     5761 gcgatagcgc ggtccaggcc gaccagttat tggcgtccac cagggcatcg tgcatttccc
     5821 agaatttatc ctggagggcg gcggcttcgg cagcctgggc agccaattgg gctttctcat
     5881 ggatgctgat gaggggcagg ttgcggaaaa tcaccgtgac ctcttcggga taggcatcca
     5941 ggaaggcctt gatctgcggc tcaagctggg cgcagtacgg gcactggaaa tcgctataga
     6001 cgatcaatgt catgcggcca tctttggcgc cgcggatgtg atcttccgct tcgggaagtg
     6061 ggatggtgat ctcgtaggca gcgcggggtt cagggaagat gctggcaacc tggcaatcgg
     6121 gcacggttgc gccttcctga acgaccggcg tagcagtggc gggaaccggc gtgggaatag
     6181 ccgtgggttc ggcctggatc atgccatcac aggcagccag cagcaggctg agcatcacaa
     6241 gcagggggaa tacaaacttc ttcaaagcga cctaaccttt cttagaaaga tcaataatga
     6301 ggtatacgtc cggcggctgc tacacatcgc agcgcaaccg gttcagtacc agttgagata
     6361 gggctgattg taccacgtct gggggctgcg aggcatccac cacccgccag cgggtcggct
     6421 cttgatgcgc catggtcagg taaccctggc gcacacgctg gtgaaattcc agcgcgtagg
     6481 catccaaacg attccattcg ccgccgctgg atcgccgccg gctcaggccg actgcggggt
     6541 ccacatccag caggagggtc aggtcggggc gcaggccgcc ggtggcaaag tcgagcagca
     6601 ggcggagcag cgccagatca acgccatgcc cgtaaccctg ataggccagg gtggagtcgg
     6661 cgtagcggtc tgagaggatg attttgccct gcgccagcgc cgggcggatg acttcttcaa
     6721 ccagttgggc acgggcggcc aggaagagca aaatttcggt gcggggctgc atggcagtat
     6781 tatccaggcg agtcagtacg gcgcgcacct ggtcaccaat gatggtgccg ccaggttcgc
     6841 gggtggttag aacatcatag ccctgttcgc gcagataatc agccagcctg ggcagctgcg
     6901 aggttttacc gctgccttca ggcccctcca gtgtgatgaa catgcgttac tcccggaaaa
     6961 tccgcacgcg gcggtgcggt tccttgccat aagaatgaga gaccaactgc gcctggcggg
     7021 ccatttcgtg ctgcatgcgc cgcacctggg gcgaggccgg gggcagatca atccattttt
     7081 gtccgttcaa gaccgcctga atggccgcag ccgtctgcgc cgagacaaaa tcccactcag
     7141 cagggccgtt atcgggggtg gcggccaggt taaagagttc agccagcgat tgttccattt
     7201 gactgaccgt gttggcgcgc agcacataaa tgggcatgcc gcgctgttca gcatccacaa
     7261 tcgtttgctc atgcgagcgg taataagacc gcagggtcat cagcacatcg gcttcggcaa
     7321 tatcctggac cacaatggca ggcaccccca cccggcgggc ggcctgagtg aggcggttgc
     7381 gggccacgcc gtaggcataa acccgcaccg ggtttaacgg ctctcgccct ggacgggcag
     7441 caggctcata ttcggggcgg ggcgaacggg ccgggcggct gggggcatag ggctcttctt
     7501 ccaggccatt ggcataggcg cggcgggaac cctgattgag ggctcgggag gctggtggcg
     7561 ggggggcggg tttttcgacc ttgatttcgc catcggcacc gcggctgcgg atttcgggtg
     7621 gcagcggggc gccgcgcacc agggcgtcga ccgcggctga gacatcgggg tgcacggcca
     7681 tctggtcgcg ctcctgaatt tcaatcagca catcaaaggt cgggggagag cggcgttcca
     7741 gcacagtttt ctgggtgccg cggcggcggg cttcttcatc ggagagggtg acggcttcaa
     7801 taccgccgat caggtcagaa agggtggggt tgagcagcag gttttccagc gtgcggccat
     7861 gcgccgtagc caccaactgc acaccgcgtt cggcaatggt gcgggcggcc tgggcttcca
     7921 gctcgcggcc aatttcatca atgacgatga cttcggggtt gtggttttcc actgcctcaa
     7981 tcatcacttc gtgctggagg gaaggcgcag ccacttgcat gcggcgggcg cggcccactg
     8041 cggggtgggg cacatcgcca tcgccgccaa tttcattgga ggtatctacg atgatgacgc
     8101 gcttggtctc cgcgaggatg cgggcagcct cgcggagcat ggtggttttg ccaatgccgg
     8161 ggcggcccag cagcaaaatg ctctgaccgg attcgaccag gtcctggata atatcaatgg
     8221 tgccatagac cgcccggccc acccggcagg tgaggccaac gatatggccg cggcggttgc
     8281 gaatggcgga gatgcggtgc agggtgcgct ccaacccggc gcggttgtcg gcatcgaaat
     8341 cgccaatgcg ggcgacaacg gtttcgattt ctgccgaggt aacttccacg tcgctaaggg
     8401 ttacttcttc atttaaaaag cgagccttgg gcagccgccc tagatcgagg acaatttcga
     8461 tcagggcgtc ttcgtggttg gcgcgggtaa ccgcctgggc aattgctgga ggcaaaaccc
     8521 ccagcagggt atgtaaatca tctgttatgc gtctctgtgt catacagttc cgattgtact
     8581 cgtttattgc cattaatggt atgggcggtg ggcgttgggc ccgcccagcc cttctccagg
     8641 gcggcctggg cagttgccgg ttggagggag cggctgaaga ccaccaggtg ccgcaccatc
     8701 aaggagtgac gggtgctggc gggtacgccg cgggcttcca gcaggggttc gagccaggcc
     8761 tggtaagagc gcaccgccag atagacgggg cggcccagcc agcggtcggg ataggccagc
     8821 agggcatcga gcagggcacc ggcatcgctg acttctggat ggatgagcgg ctgaaggcat
     8881 acgccgcgcg gcccggattg gacatcgaca taggcgcgca gagcaccaga ctgccagtga
     8941 acccaaccgg aggcaaaccg gggttcgaag gcttcggcgg cctgcaccag cggcggggtg
     9001 agctgttgat gcagactgcg gacggcgttc tcatcggcgg cgcggtaggg ccgccaggag
     9061 agatctgccg cggcttgcgg ggcggcggcc acccgccagt aacgctgcca ggcatagacg
     9121 gcaaagcccg accggcggag cagttcaaaa gtgcccaggt catcggcgga ctcggccagc
     9181 aggcacaggg cgccgcgctc acctgcggcc gcggccaggc tatccagcag cactgcgccg
     9241 ggggtggatt caagggcatc ctggggcatg aggaaggtga ggtgggcggc gcgctgcccc
     9301 agtgtgtagc gcatgcggcc cagcaggcgg ctacgcccta attccactac ggcggcagag
     9361 tcgccataat cgcccaggcg caggtgacgc agcagcaagc ccagaccaat ggggctgccg
     9421 tgcgtcaacc gacccgtttg atccaggcaa accacctgcc gccggaaacg gtagacgttc
     9481 agtaaatcag gccaggcaaa cggacggacc tctatgctgc cagctccata aagtaacggt
     9541 gaaagcggtc atcggcggtc agttcgggat gaaaagcggt ggcgagcagg cggccctgct
     9601 gcgcggccac aattttaccg cccaggcccg gacgacgacc cgcaaaatcc ccgggaaggg
     9661 cagccagcgg ctgcacaccg ggaccgatgg attcgatcag tggggcgcgg atgaatacag
     9721 cgtggaatgg gcgctcgggt tcaggcaggg cgttgatggt caggtcggcc tcaaagctct
     9781 cagtctgacg gccaaaggcg ttgcgctcga cggtgatgtc catcaatccc agcaggggtt
     9841 gagcgcggcg ggcatcgcgc gagagcagga tggcgcccgc gcaggtgccc cagatggcat
     9901 gggtttggcc gaaggtacgc agcggctcca gcaggccaaa atccaccgcc agcttgccca
     9961 tggtggtaga ctcgccgccg ggaatgatca gaccatcgag gccagccaac tcatcagcca
    10021 ggcgcacctg agtcaccgag gcgcccaggc gcttcagcac aacctgatgt tcggcaaaag
    10081 cgccctgcag cgccagaaca ccaatctgca aagccattta ccagcctcgc ccggccatgc
    10141 gttcagcatc gggcaggttt gccacggtgc ggcccaccat cggctcgccc aggttgcggc
    10201 tgacctccgc caggatggcg gcgtcggtgt agtgcgtgac ggctttgaca atggcggcgg
    10261 cgcgggtggc cgggttgccc gacttgaaga tgcccgagcc cacaaagacg ccgtccacgc
    10321 caagctgcat catcagagcg gcatcggcag gggtggcaat gccaccagca gcaaaattga
    10381 ccaccggcag gcggcccagc gagcgggttt gaaccagcag ttcatagggc gcgccaagtt
    10441 ccttggcata ggtcatcagt tcttcttcgg gcatggtttg caggcggcga atatcgccca
    10501 aaacctggcg ggcatggcgt acggcctcga ccacatcgcc ggtgccggct tcgcctttgg
    10561 tgcgcatcat ggctgcgcct tcgccaatgc ggcgcagggc ttcgcccagg ttgcggcagc
    10621 cgcagacaaa cggcacattg aaagcgtgct tgtaaatgtg gaacgcctca tcggcggggg
    10681 tcaggacttc ggattcgtca atataatcca cgccgagcga ctgcaagatt tgcgcctcaa
    10741 caaaatgacc gatgcggcat ttagccatta cggggatgga gacggaatcc atgattttga
    10801 ggatgagttc gggatcgctc atgcgagcca cgccgccctg ggcgcggata tcggcgggga
    10861 cgcgttcgag cgccattacg gcgcaggcac cggcttcttc ggcaatccgg gcctgttcgg
    10921 gggtgaccac atccataatc acgccgcctt taagcatttg agccagtccc tgcttgaccg
    10981 caaaggtgcc gacctgttgt tccatacgta aacctccaaa agattgaatt tgcgttttga
    11041 ttctaccacg gggcaagggg taatttttta attttagat
//
LOCUS       DAOPVS010000056        10969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6039_length_10969_cov_58.921477, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000056 DAOPVS010000000
VERSION     DAOPVS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6039_length_10969_cov_58.921477"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2302)
                     /gene="recJ"
                     /locus_tag="PKL11_06245"
     CDS             complement(<1..2302)
                     /gene="recJ"
                     /locus_tag="PKL11_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNZ13240.1"
                     /translation="MSAATIWIEPAPVEVPAELAEFVGGHALISEALVRRGIQSIEAA
                     RRFLFAEHYPVTNPLELPDLGNATERMMQALKNGERIGVWGDFDVDGQTATTLLVAGL
                     RALGGSVIHHIPVRGRETHGIQLAALKEFLAQGVEVILTCDTGVTAHESIDYAQSMGV
                     DVVVTDHHTLAETLPAARAVVNPQRLPEGHSLRTLCGAGVAYQLLMEICQQAGRPEVA
                     ADQLDLAALGTVADLAILTGDSRWIVQKGLERLREHPRPAIQAMLELAGAQAEQLNEQ
                     HISFMLAPRLNALGRLDDANPIVDFLTASEIAAARQMAVHLEGLNAQRRLLCDQVFRA
                     AQAQIAQNPAVLDEAVLILAHPEWPASVVGIVASRLVELYQLPTLLLHAPPGEAARGS
                     ARSVEGINITAAIAANQHLLLGFGGHPMAAGLSLAEEKLPEFRRALARTVRQMRAGER
                     PAAEMAIDAFLPLTDLSLELSEDIARLAPFGPGNPPLTLATRNLMLRSWSFIGRTQEH
                     LQLLVEDEAGNTRKVLWWQGAGSPLPEGRFDLAYTLQASSFRGQAALQVEWLHARQIE
                     GPGIGITAAPKPLEIVDLRTAENPAEQLSQLDPGNAGMLWVEGADKAHLPGSDRYHLT
                     PAETLVVWNLPPGPLELQIAVQTVNPQRVIFVGITPPDGQINDFLKRLMGLVQYAIRQ
                     RQGWAAWSELAAAMAQREVTVQAGIKWLAAQGKIQIGATQLGRVQIEAGGAPALPGLQ
                     ASADRDLKALLNETAAFRAYLRRASLE"
     gene            complement(2302..3123)
                     /locus_tag="PKL11_06250"
     CDS             complement(2302..3123)
                     /locus_tag="PKL11_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNZ13241.1"
                     /translation="MPLPGNFHFSQNNLQDFVDCARRFELRHLLKLAWPAPQTEPVLQ
                     AEQDMLLGNQFHHLIHQHQIGLAAETLLPGDLQLQNWWQAYLDYAPHQLPGQHYPEFT
                     LSAPFGTHRLIAKFDLLCIVPGEKAVIVDWKTSRRLPSHNTLMERIQTRLYRLLLVEG
                     GAYLNGDQPIQTEQIEMIYWFANEPQNLEKLAYSPAQAEADRQEIQARIEEIVQLSHL
                     ERPFPLTLDERRCAYCNYRSLCNRGERAGSGEMDEFELDDGPLTLDFDQIGEIAF"
     gene            complement(3107..5362)
                     /locus_tag="PKL11_06255"
     CDS             complement(3107..5362)
                     /locus_tag="PKL11_06255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNZ13242.1"
                     /translation="MSAVLTFQPRIMQREVLRYTTGRMGVSAVPGSGKTHTLSALAAR
                     LIADGWVAEYQEILIVTLANSAVNNFAYRINEFIKAYGLIPGVGYRVRTLHSLAHEIV
                     RERPDLVGLSDRFEIVDERESGEILRSVVTNWMRANPEFSAEWLNPEIDEARAHDANR
                     QWGDTLISLAGALIKKSKDLMTTPQELRTKLNGLQRTPSLLRFAADVYADYQRALNFR
                     SAVDFDDLIRLALQALQTDGDYLERLRARWPYILEDESQDSSELQERILRLLTGPEGN
                     WVRVGDPNQAIYETFTTASPHFLLDFRKETGVLAPNLAESGRSTKSIIGLANHLISWT
                     REAHPVRELREALTLPYILPTQPDDPQPNPEDQPEGIKLINTRYTPEKEIEAIVRSLK
                     HWLPEHSDWTVAVLTPRNERGAKVVEALQEAGIEVVELLQSSQSTRETAEMLAAVLKA
                     LADPTAPRKLADVYTRLPLPPEVDPAQGTALKAAGADVLQKCANLEDFIWPLPGSDWL
                     EMLAGGPEIIRDLMAFRSYIQRWQAAVLLPVDQLVLTIATDIFGNPSDLAMAHKLALL
                     LERAGALHPEWQLPDFANELGEISRNRRKLLGFGEEDSGFDPDRHRGKVVVATIHKAK
                     GLEWDRVYLMSVNNYDFPGAQPYDRYISERWYVRGSLNLEAEGLAQLEALIRGDVAGI
                     YMEEGAATQEARFDYSAERLRLLYVGITRAKRELIVTWNTGKIIKSEAQPATALVELQ
                     AFWEKKHAAAG"
     gene            complement(5359..7458)
                     /locus_tag="PKL11_06260"
     CDS             complement(5359..7458)
                     /locus_tag="PKL11_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13243.1"
                     /translation="MTMKLTPEQNALTHRPLDHKLFLRGPAGTGKTTIGINRTLALLN
                     WRVPAERILVLVPQRTLGLAYTEALREPSLPPGGQVTVVTLGGLAQRMIGLFWPLIAR
                     EAGFAHPEQGPTFLTLETSQYYMARLVRPLIDEGYFESITIDRNRLYSQIVDNLNKTA
                     VTGLDYRSFGERLRAAWLGDPGQLNVYDQAQECANRFRQFCLDNNLLDFSLQMEVFVR
                     RIWPAFMCREYMTRTYRHLIYDNAEEDVPVVHDVIQQWLPAFDSALVIYDDGGGYRAF
                     LGADPESALRLQDECAETAELVESWITPPPMAALADHLGTCLMRQPAEPPTETRLAFR
                     LEHHRFYPEMVDTVCAEVAGLVQEQEIAPGEIVILSAYLSDALRFSIMNGLQARGIPA
                     RSHRPSRSLREEPATACLLTLAMLAHPEWGLAVSKFDVRNALLQAIEGLDLVRADLMA
                     QILYLPKQPEAPLAAFDKIRMDKQQRITYTIGERYERLRSWLQAARERQIEALDIFIA
                     QLFGELLSQPGFGFHINYDAAAITSRLIESIEKFRRVTAPMLAEEKAAVSQEYLKMVQ
                     EGVLAAQYLQPWEQPEQDSVMVSPAYTFLMTNRPATVQFWLDVGSQGWWERLYQPLTH
                     PVVLSRRWTGDGPWTDANEFQVNQETLAKLASGLLRRCRGEVVFCTTGANERGDEERG
                     PLLQGIQALVRRLRILE"
     gene            7592..7981
                     /locus_tag="PKL11_06265"
     CDS             7592..7981
                     /locus_tag="PKL11_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13244.1"
                     /translation="MRKPLLILAFLTVVVLLSACTEQKSPAVQAVEAYQTAFASKDAD
                     ALAQLSCADWEAQALLEMDSFQAVGTVLDSVACTEAGAEGDAVLVTCEGSISLTYGEE
                     KRSFDLSGRTFTVIQEGADWLVCGAQP"
     gene            7978..8106
                     /locus_tag="PKL11_06270"
     CDS             7978..8106
                     /locus_tag="PKL11_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13245.1"
                     /translation="MKKVLRWLFNRETLWALLLCLIIIAVVIFTTDSSPLWIYQGF"
     gene            8107..9531
                     /locus_tag="PKL11_06275"
     CDS             8107..9531
                     /locus_tag="PKL11_06275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNZ13246.1"
                     /translation="MTLTNILIFAALILAYRLILRERWRGWLLFGVSVIAVYWLQPAL
                     PIRGLDFWLPTATLALVVFGWAATAPQEDRRTRENWVTAVALLALVLLIATTRYLSLT
                     GLLTPSRPPQTVSVLVVLAIIALIGWLLVRFTRPVSAGLTAFIVVIILVFLALKIPQV
                     TQPLSALFRSLAGQSVERASAIDIRWLGFSYVAFRLIATLRDRQTGRLPSITLREYIT
                     YLIFFPALTAGPIDRPERFLKDLRGSHPPSASEFLIGGQRLAVGLFKKFVVADALALL
                     ALSSANADKIQTAGWLWVAVAAYSLLIYFDFSGYTDIAIGLARWLGIALPENFNHPYL
                     KPNLTQFWNNWHMTLTQWFRAYFFNPLTRALRSAPRPVSVVWVILITQVSTFLLIGLW
                     HGGTPNFLLWGLWHGLGQFIQNRYSDFMKPRIAGLDNRPTLKRVLSIAGTALTFIYVT
                     LGWVWFALPTTGQALQVFTRLFGG"
     gene            9584..10819
                     /locus_tag="PKL11_06280"
     CDS             9584..10819
                     /locus_tag="PKL11_06280"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNZ13247.1"
                     /translation="MNAKNSARWSLLCGLVLVSLLFSACNSWGAAGHATQTAVSLTQT
                     ETARPTNTPTSTATPTQTPTPTATPTATPTPTPTPVVYGPTGFPDNVNPLTGLVVSDP
                     ELLNRRPMFIKVANFPRTGRPHAGLSYADLVFEYYIGEGANRFAAVYYGQDCPQVGPM
                     RSGRLVDAQLAPMYQGTLAYVSAWDWVRTKLDQTMQWRGMLEGPNTCPAICRLPPGDV
                     TSVFADTAALEEAFAKRNVQIEDPVLDGMYFNEAVPANGIPGEKLRVEFYIKNIGEWV
                     YDPVSGKYLRWIETVDVVTGEVNGMIPLVDRLTDEQLGFSNVVLIFATYTTYSPTIHD
                     MTILDNRDGMRAVLFRDGQAFETVWKSVGYDKPIQFFTPEGEPMAFKPGNTWFVIMGR
                     NSPYTETEPGKWLVQLSLR"
BASE COUNT         2080 a         3605 c         2832 g         2452 t
ORIGIN      
        1 cttctaaaga agctcttctc aaatacgccc gaaaagctgc tgtttcattc agcaatgcct
       61 tgagatccct gtccgcgctg gcctgcaggc cgggtagagc cggggcgcca cctgcctcaa
      121 tttgaacgcg ccctagttgc gtagccccaa tctgaatttt cccttgcgct gccagccact
      181 taatccctgc ctgcacggtc acttcgcgct gcgccattgc cgcggccagt tcagaccacg
      241 cggcccagcc ttgccgctgc cggatggcat attgaaccag ccccatcagg cgcttcagaa
      301 agtcgttgat ctgcccatcc gggggcgtta tccccacaaa aatgactcgc tgcggattca
      361 ctgtttgcac cgcaatctgc agttccagcg gtccaggagg caggttccag acaaccagtg
      421 tctccgccgg ggtcaggtga taccggtccg atcccggcag gtgcgcttta tcggccccct
      481 caacccacaa cattcccgca ttacccgggt ccaattggct caattgctct gccggatttt
      541 ctgccgttct caagtccaca atctcaagcg gttttggtgc cgccgtgata ccgatacccg
      601 gcccttcaat ttgccgggcg tgcagccatt caacctgcaa ggccgcctgt cccctgaagc
      661 tggacgcctg cagggtgtat gccaggtcaa agcgcccttc gggcagcggt gaaccggccc
      721 cctgccacca cagcaccttg cgcgtgttcc cggcctcatc ttccaccagc agttgcagat
      781 gctcctgcgt tcttccaata aagctccacg agcgcagcat caggttgcgg gttgccagcg
      841 tcaggggcgg gttccccggt ccaaatggcg ccagtcgggc aatgtcctcg ctgagctcca
      901 gcgagaggtc ggtcagcggc aggaaggcat caattgccat ctctgccgcc gggcgttctc
      961 ctgcccgcat ttgccggacg gtgcgcgcca gcgcccgcct gaattcgggc agcttttcct
     1021 ctgccagtga taaccctgcc gccatcggat gccccccaaa ccccagcagc aaatgctggt
     1081 tggccgcaat cgccgccgtg atgttgatcc cttccaccga ccgcgccgaa ccccgggcgg
     1141 cctcgccagg tggggcatgt aataatagcg tgggtagttg atacagctcc accagccggc
     1201 tggcaacaat ccccaccacg ctggccggcc attccgggtg ggccagaatc aataccgctt
     1261 catccagcac cgccggattt tgggcaatct gcgcctgcgc tgctcgaaac acctggtcgc
     1321 acagcagccg gcgttgggcg ttcagccctt ccaggtgcac tgccatctgg cgcgctgcgg
     1381 caatttcgct ggcggtcaaa aaatccacaa ttggattggc gtcatccagc cgtcccaggg
     1441 cattcaggcg cggcgccagc ataaaactga tatgctgttc attgagctgt tctgcctgtg
     1501 cgccggccag ttccagcatc gcctgaattg ccgggcgggg gtgttctcgc agccgctcca
     1561 ggcctttctg cacaatccaa cggctgtcgc cggtcaaaat agccagatcc gccacagttc
     1621 ccaacgctgc caggtcaagc tgatctgcag cgacctctgg cctgcctgcc tgctggcaga
     1681 tttccatcag tagttgatag gccactcccg cgccgcacaa ggttctcagg gaatgcccct
     1741 ccggtagccg ctgcggattg acaaccgccc gcgccgccgg cagcgtttct gccagcgtat
     1801 gatgatccgt aaccactaca tccacgccca tagactgcgc atagtcaatc gattcgtgcg
     1861 ctgtgacgcc cgtgtcgcac gtcaggataa cttcaacccc ctgcgccaga aattctttca
     1921 gcgccgccag ttgaattccg tgcgtctcgc gtcctcgcac cgggatatga tgaataacgc
     1981 tgccccccag cgctcgcagc cccgcaacca gcagggtggt ggcagtctgc ccatccacgt
     2041 caaaatctcc ccaaacccca atccgctcgc cgttcttcag cgcttgcatc atccgttcag
     2101 tcgcattgcc caggtcgggc agttccagcg gattggtgac cgggtaatgc tcggcaaaca
     2161 ggaatcgccg cgcggcctca atgctttgaa tccctcgccg gaccagtgct tcgctgatga
     2221 gcgcatgccc ccccacaaat tcggccaatt cagcgggtac ctccactggc gccggctcaa
     2281 tccagatcgt tgctgcgctc attagaaagc aatctcacca atctggtcaa agtccagggt
     2341 cagcggtccg tcatccagct caaattcgtc catctcgccg cttcccgcac gctctccccg
     2401 attgcaaagt gagcggtaat tgcagtaggc gcaccgccgt tcatccagcg tcagcggaaa
     2461 cggccgctct aaatggctta gctggacaat ttcctcaatt cgcgcctgaa tttcctgccg
     2521 gtctgcctct gcctgcgccg ggctgtaagc cagcttttcc agattttggg gctcgttggc
     2581 aaaccagtag atcatttcaa tctgctctgt ctgaataggc tgatcgccat tcaaataggc
     2641 gccgccctct accagcagta gccggtacag cctggtctga atgcgctcca tcagggtatt
     2701 gtgactgggt agccgccgtg aggttttcca atccacgatg acagctttct cacccggcac
     2761 gatgcacagt aaatcaaact tggcaatcag ccggtgggtg ccaaagggcg cgctgagggt
     2821 aaattctggg taatgctgcc ccggcagctg gtgaggtgca taatccaggt atgcctgcca
     2881 ccagttctgc agctgcaggt cgccgggcag cagtgtttct gcggccagcc caatctgatg
     2941 ttggtgaata agatgatgaa actgattgcc cagcagcata tcctgctcag cctgcagcac
     3001 cggctctgtt tgcggcgccg gccaggccag cttcagcaaa tgccgcagtt caaaccgccg
     3061 ggcgcagtcc acaaaatcct gcaggttgtt ctggctgaaa tgaaaattac ccggcagcgg
     3121 catgtttttt ctcccagaag gcttgcagtt ccaccagtgc agtggctggc tgcgcttcac
     3181 tcttgataat ttttcccgtg ttccaggtca caatcagctc tcgctttgcc cgggtgatgc
     3241 caacgtacag cagccgcagc cgttcggcgc tgtaatcaaa ccgggcttcc tgcgtggcgg
     3301 ccccttcttc catgtatatc cccgccacat ccccgcggat cagtgcttct aactgtgcca
     3361 aaccctcagc ttccaggttc aacgatccgc gcacatacca gcgctcgctg atgtaccggt
     3421 cataaggctg cgcgcccgga aaatcataat tattcaccga catcaaataa acccgatccc
     3481 actccagccc cttggcctta tgaatcgttg ccaccaccac cttgcctcgg tgtcggtcgg
     3541 ggtcaaaccc gctgtcttct tcgccaaacc ccagcagctt gcgccggttg cgtgaaatct
     3601 ctcccagttc gttggcaaaa tcgggcaact gccattcggg gtgcagcgct ccggcgcgct
     3661 ccagcagcag cgccagctta tgcgccatcg ccaggtcaga cggattgcca aaaatatcgg
     3721 ttgcaatcgt cagcaccagt tgatccaccg gcagcagcac agccgcctgc caccgctgga
     3781 tatagctccg aaatgccatc aaatcccgga tgatctccgg gccgcccgcc agcatttcca
     3841 accagtcgct ccccggcagc ggccagataa aatcttccag gttggcgcac ttttgcagca
     3901 catcggcccc ggctgctttg agtgccgttc cctgcgccgg atcgacctcc ggggggagtg
     3961 gcagccgggt ataaacatcc gccagcttgc gcggggcggt ggggtcagcc agtgccttca
     4021 gcacggccgc cagcatctcc gccgtctctc gggtggattg actgctctgc agcagctcca
     4081 caacctcaat cccggcttcc tgcaaggcct caaccacttt ggcgccgcgc tcgttgcgcg
     4141 gggtcaatac tgccacagtc caatcactat gttctggcag ccagtgtttc aggctgcgca
     4201 caatcgcctc aatttccttt tccggtgtgt accgggtgtt gattaacttg atcccttccg
     4261 gctgatcttc cgggttgggt tgtggatcat ccggctgcgt cggcagaata taaggcaggg
     4321 tcagtgcctc gcgcagctcc cgcaccggat gcgcctcacg cgtccagcta atcaggtgat
     4381 ttgccaaccc aataatgctc tttgtggatc gccccgactc cgccagattg ggcgccagca
     4441 cacccgtctc cttccggaaa tccagcagaa agtgcgggct ggcagtggtg aacgtttcgt
     4501 aaatcgcctg gttgggatcg cccacccgaa cccagttacc ctccggcccc gtcagcagcc
     4561 gtagaatccg ctcctgcaat tcgctgctgt cctgagattc gtcttccaat atataaggcc
     4621 agcgcgcgcg cagccgttcc agataatccc catcggtttg cagggcttgc agtgccaacc
     4681 ggatcaagtc atcgaaatcc accgcactcc gaaaattgag cgcccgttga taatcggcat
     4741 agacatctgc tgcaaatcgc agcaggctcg gggtccgttg caatccattc agttttgtac
     4801 gcagttcctg aggggtggtc atcaaatctt tagatttctt gatcagcgct cccgccagcg
     4861 agatcagcgt gtcgccccat tgccggttgg cgtcgtgcgc ccgggcttcg tcaatctccg
     4921 gattcagcca ctcagccgaa aactccggat tggcgcgcat ccagttggtc accacactgc
     4981 gcaaaatctc gccgctctcg cgctcatcta caatctcaaa tcggtccgat aaccccacca
     5041 ggtccggtcg ctcccgtaca atctcgtgtg ccaggctgtg cagcgtccgc acccggtatc
     5101 ccactcccgg aatcaaccca taagctttga taaattcatt gatccgatag gcaaaattat
     5161 taaccgctga atttgccagc gtaacgatca aaatctcctg atattcagcc acccagccgt
     5221 ccgcaatcag tctggctgcc agcgctgaca gtgtatgcgt cttgccgctg cccggcaccg
     5281 ccgagacgcc catgcgcccg gtggtgtacc gcagcacttc ccgctgcatg attcttggct
     5341 gaaaggttaa caccgcgctc attccagtat cctcaatcgc cgcaccagcg cctgaatacc
     5401 ctgcagcagc gggccgcgct cttcgtcgcc gcgctcgttg gcgcctgtgg tgcagaacac
     5461 cacttcgccc cggcagcgcc gcagcaaacc acttgccagt tttgccagcg tctcttgatt
     5521 aacctgaaat tcattggcat cggtccacgg tccatcgcca gtccagcgcc ggctcagcac
     5581 caccgggtgc gtcagcggct ggtacagccg ctcccaccaa ccctggctgc ccacgtccag
     5641 ccagaattgc actgttgccg ggcggttggt catcaaaaac gtgtaggccg gcgagaccat
     5701 cacactgtct tgttctggct gctcccaggg ctgcaaatac tgcgccgcca gcacgccctc
     5761 ttgcaccatc ttcaaatatt cctggctgac cgcagccttc tcttccgcca gcattggcgc
     5821 tgtcacccgg cgaaactttt cgatggattc aatcaagcgg ctcgtgatgg ccgcagcatc
     5881 gtaattgatg tgaaatccaa agcctggctg cgacagcagc tcgccaaaca actgtgcgat
     5941 aaagatatcc aacgcctcaa tctgtcgttc tcttgccgct tgcagccagc ttcgcagccg
     6001 ctcatagcgt tctccaatgg tgtaggtgat acgctgctgc ttgtccattc tgatcttgtc
     6061 aaacgctgcc agcggcgctt cgggctgctt gggcagatac agtatctgcg ccatcaaatc
     6121 tgcccgcacc aggtccagcc cttcaatcgc ctgcagcagt gcgttgcgca catcaaattt
     6181 ggagaccgcc agtccccatt ccgggtgcgc cagcatcgcc agggtcagca ggcaggctgt
     6241 ggcgggctct tcacgcaaac tgcgtgaggg ccgatgagac cgcgctggaa tcccccgggc
     6301 ttgcagtcca ttcataattg aaaaccgcag ggcgtcgctc aggtatgccg acaaaattac
     6361 aatctcgccc ggtgcaatct cctgttcctg caccagcccg gcaacctctg cgcagacggt
     6421 gtccaccatc tccgggtaaa accggtgatg ctccaaccga aatgccagcc gcgtttcggt
     6481 cggcggctct gctggctgtc gcatcaggca ggttcccagg tggtcagcca gtgcagccat
     6541 tggcggcggc gtaatccagc tctcaaccag ttcggcagtt tcggcacatt cgtcttgcag
     6601 ccgcagtgcc gattccgggt ccgcccccag gaacgctcga taccccccgc cgtcgtcata
     6661 aatcaccagc gccgaatcaa acgctggcag ccactgttga ataacatcat gcaccaccgg
     6721 cacatcttct tcggcattgt cataaatcaa atgccggtag gtgcgggtca tatattcccg
     6781 gcacataaac gccggccaga tccgccggac aaacacctcc atttgaagtg aaaagtccag
     6841 caggttatta tccaggcaaa actgccggaa gcggttggcg cattcctgtg cctgatcata
     6901 cacattcaat tgccccgggt cgccaagcca cgccgcccgc agtcgttctc caaatgagcg
     6961 ataatccagc cctgtgacgg cggtcttatt cagattatcc acaatctggc tgtacaaccg
     7021 gttgcggtca attgtaatgc tctcgaaata accctcatca atcagaggcc gcaccagccg
     7081 cgccatatag tactgcgaag tctccagcgt caagaaggtc ggcccctgtt ccgggtgggc
     7141 aaaccccgcc tcgcgcgcaa tcaggggcca gaagagacca atcatccgtt gtgccagacc
     7201 gcccagcgtc accaccgtca cctgtccccc cggcggtagg ctcggctctc gcagcgcctc
     7261 tgtgtaagcc agtcccagcg ttcgctgtgg caccagcacc agaatccgct cggctggcac
     7321 ccgccagttg agcagtgcca acgtccggtt gatcccaatg gtcgttttgc ctgtcccggc
     7381 tggcccacgc aggaagagct tgtgatccag cgggcggtgg gtgagggcat tttgttcggg
     7441 cgtcagtttc atcgtcatta ctgtatcata aacccccatt tagcacaatt ctgagcagtg
     7501 taacatcctc aatcggtaca tgatataatc tgcgcgacgt attccagcgc ataagctgtg
     7561 tggatgcacg gctgattcaa acgaggcaaa catgcgaaaa ccgctcctca ttttagcatt
     7621 tttgacagtc gtggtgctgc tttctgcctg caccgagcag aaaagcccgg ctgtccaggc
     7681 tgttgaagcc taccagactg cctttgcaag caaagatgcc gacgccctgg cccagctctc
     7741 ctgcgcagac tgggaagccc aggccctgct tgaaatggac tcctttcagg ctgttggcac
     7801 cgtgctggat tcggtcgcct gcactgaagc cggtgcagaa ggtgatgcgg ttctggtcac
     7861 ctgtgaaggc agcatcagcc tgacctacgg cgaagagaaa cgcagctttg acctttcagg
     7921 gcgcaccttc accgtcattc aagaaggcgc cgattggctt gtctgcggag cccagccgtg
     7981 aaaaaggtgc tgcgctggct ctttaatcgc gagacgcttt gggcgctgct gctctgcctg
     8041 atcattatcg ctgtggtcat tttcaccacc gatagttccc ctctttggat ttaccagggc
     8101 ttctaaatga ccctgaccaa cattctcatc tttgcggcac tcatcctggc ctaccgcctc
     8161 atcctgcggg aacgctggcg cggctggctc ctgtttggcg tcagcgtcat cgccgtctac
     8221 tggctccagc ccgccctgcc catccggggc ctggactttt ggctgcctac tgccaccctg
     8281 gcgctggttg tctttggctg ggcagccacc gcgcctcagg aagatcgccg cacccgcgaa
     8341 aattgggtca ctgcggttgc cctccttgcc ctggtcttgt tgatcgcgac cacgcgctac
     8401 ctcagcctga cgggcctcct caccccgtcc cgcccaccgc agaccgtttc agtgcttgtc
     8461 gtcctggcga tcattgccct cattggctgg ctgctggtgc gtttcacccg ccccgtttct
     8521 gccggactta cggcttttat cgtagtcatt atcctggtct ttttagccct taaaattcct
     8581 caggtcacac agcccctcag cgccctgttc cgctccctgg cggggcagtc ggtggagcgt
     8641 gccagcgcca ttgatattcg ttggctgggc ttctcctatg tcgccttccg cctcatcgcc
     8701 accctgcgag atcgtcagac ggggcgtctg ccttccatca ccctgcgtga atatatcacc
     8761 tatctcattt tcttccccgc cctgaccgcc ggacccattg accgccccga gcgctttctc
     8821 aaagacctgc gcggcagtca tccaccgtcc gcctctgaat ttctgattgg tggtcagcgt
     8881 ctggctgttg gcctgttcaa gaaatttgtg gttgccgatg ccctggcgct cctggcgctt
     8941 tcatctgcca atgccgacaa aatccagacc gctggctggc tctgggtggc cgtggcggcc
     9001 tattcgctgc tcatttattt cgatttctcg ggctataccg atattgccat tggtctggcg
     9061 cgctggctgg gcattgccct gcccgagaac tttaatcatc cctacctcaa acccaacctt
     9121 acccaattct ggaacaactg gcacatgacg ctcacccagt ggttccgggc gtacttcttc
     9181 aatccgctca cccgggcgct gcgcagcgcc ccccgcccgg tgagtgtggt ctgggtgatt
     9241 ttgattaccc aggtcagcac ctttttgctc atcggcctct ggcatggcgg cacgcctaat
     9301 ttcctgctct gggggctctg gcatggcctg ggtcagttta tccagaaccg ttacagtgat
     9361 ttcatgaaac cccgcattgc cgggttggat aatcgcccaa ccctcaaacg cgtattgagc
     9421 attgctggca cagcgctcac attcatctat gtcacgttag gctgggtttg gtttgccctg
     9481 cccaccaccg ggcaggcgct tcaggtcttt acccggcttt tcggcggtta acagcatcca
     9541 gagtataata tctggcttga tgcttgatta ttgaggagat tgtatgaacg ccaaaaattc
     9601 agcccgttgg tctttgctgt gtgggttggt tctggtttcc ttgttgttct ctgcctgcaa
     9661 tagttggggc gcagccggcc atgccacgca gacggccgtc agcctcactc agacggagac
     9721 tgcccgccca accaataccc ccacatctac tgccacgccc acccagacgc cgacccccac
     9781 cgctacccct accgctacgc cgacgcccac ccccacgccg gtggtttacg gccccaccgg
     9841 tttccccgat aacgtcaacc cactgaccgg tctggtggtg tctgaccccg agctgctcaa
     9901 ccgccgcccc atgtttatca aggtggctaa cttcccccgc acaggtcgcc cacatgctgg
     9961 tctgtcctat gctgacctcg tttttgaata ttacattggc gaaggcgcca atcgctttgc
    10021 cgcggtctat tatggtcagg actgccccca ggttggtccc atgcgctcag gtcgtctggt
    10081 agatgcccag cttgccccca tgtaccaggg caccctggcc tacgtcagcg cctgggattg
    10141 ggttcgcacc aagctggacc aaaccatgca gtggcgcggc atgttggaag gtcccaatac
    10201 gtgtccggcc atttgccgtc tcccacccgg cgacgtgacc agtgtatttg ccgataccgc
    10261 cgctcttgaa gaggcttttg ccaagcgcaa tgtccagatc gaagaccccg ttttggatgg
    10321 aatgtacttc aacgaagccg ttcccgccaa tggcatccct ggcgaaaaat tgcgtgtaga
    10381 gttttacatc aaaaacattg gcgaatgggt ctatgacccc gtcagcggca aatatctgcg
    10441 ctggattgag accgtggatg ttgtcaccgg cgaagtcaac ggcatgatcc ccctggtgga
    10501 ccgcctcacc gatgagcagt tgggtttctc aaacgttgtc ttgatctttg ccacatacac
    10561 cacctattcg cccaccatcc acgacatgac cattttggat aatcgggatg gtatgcgcgc
    10621 cgtgctgttc cgcgatggtc aggcctttga gaccgtctgg aaatccgtgg gctatgacaa
    10681 gcccatccag ttctttaccc ccgaaggtga acccatggcc ttcaagccgg gcaacacctg
    10741 gtttgtcatc atgggccgca actcgcccta caccgaaact gagccaggca aatggctggt
    10801 tcagctttcc ctgcgctagt ctggagctgt tgatgaaaac gaaaattctg tatcttgctt
    10861 ttgttttgag ccttgtcctg gctgcttgca ccagcaatgc cagccctgcc gccaccgcgg
    10921 tcgagcagta tcttacggcc ctggttggta agcagtccga tacgctctc
//
LOCUS       DAOPVS010000057        10907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6086_length_10907_cov_20.699963, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000057 DAOPVS010000000
VERSION     DAOPVS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_10907_cov_20.699963"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKL11_06285"
     CDS             complement(<1..287)
                     /locus_tag="PKL11_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13248.1"
                     /translation="MPSTEPTRNVKPSVAQLIRWSKLWISLPPDARAAIVNSRPGQLH
                     NAHRAIRRRWAWAVYLAGAVFFCQSYALTLPKHQLSIVVAIGFTSVIATINL"
     gene            719..928
                     /locus_tag="PKL11_06290"
     CDS             719..928
                     /locus_tag="PKL11_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13249.1"
                     /translation="MADDYMVRMNDLEAAEKLKVMAKMDKRTIGDQNAWIIHQMWALR
                     TGLPAAMMPIIPPVIVPDITSGEAK"
     gene            1192..1425
                     /locus_tag="PKL11_06295"
     CDS             1192..1425
                     /locus_tag="PKL11_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13250.1"
                     /translation="MSDKSTKNVQKRKALNVIITRRFIPVEDGEERWRYAISEILRIS
                     LRYMRQNGIPLPTTHETYPDVSVNAADPEVVES"
     gene            1422..1742
                     /locus_tag="PKL11_06300"
     CDS             1422..1742
                     /locus_tag="PKL11_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13251.1"
                     /translation="MMPEKIASLCLVLFVFGLIYNVLIDAADWPRYLVGLTGLPDAVR
                     AGIMSAGAGWGIELERWRFAWSTLAAFASQVASMVIGTVARDVRVRQTGYLCLLGEVL
                     TDER"
     gene            1732..1956
                     /locus_tag="PKL11_06305"
     CDS             1732..1956
                     /locus_tag="PKL11_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13252.1"
                     /translation="MSGDRARPWPTKAAWARDDAVQSLAEAVRMLEPMVAGERQISEA
                     EIIRRTARALVAVERAIRILAQAGAQVKID"
     gene            2101..2802
                     /locus_tag="PKL11_06310"
     CDS             2101..2802
                     /locus_tag="PKL11_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13253.1"
                     /translation="MTDLNLSDLPPELMDLYRQAAVDQSMRQHEAQERDDANEAARLN
                     RERQTLEQLIANDQLPEAVRPYVTFDWRTSTSDYRPFSLLVSLPDIAPIMVHWVWGGT
                     TNGPQPVSVKYSTPMDIEDDEDGTPTYSGYYQITDIFAEALYNAIHYAPSMKMHLNRK
                     TAEKAQRENQEQAEVDAEASQEEHDEKIRDLLMDNSYDPTRGDVGNLLKMINCRLTAI
                     GMVLQDIAIAQRGEK"
     assembly_gap    2827..2926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3099..3305
                     /locus_tag="PKL11_06315"
     CDS             3099..3305
                     /locus_tag="PKL11_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13254.1"
                     /translation="MNPNTPILRQFSFDALRICGECHEQFVLGVDGVVMEGRDLCDKC
                     AGVTRTVPGGHVILINDKPTVEVK"
     gene            3305..3643
                     /locus_tag="PKL11_06320"
     CDS             3305..3643
                     /locus_tag="PKL11_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13255.1"
                     /translation="MLPSLMTDMQIDEAWDMYLAYLGNQAERGYRLCDGCQQWYNVRH
                     MRQYAGGWLCTCCASKYDPTIAPEDTHPPVAQICGYCGDAHDSLTAKLDGVDCCESCM
                     QIVIDASTPC"
     gene            3717..4118
                     /locus_tag="PKL11_06325"
     CDS             3717..4118
                     /locus_tag="PKL11_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13256.1"
                     /translation="MTTTPFYDDLIAAMPAGLERRVFEALQVHVGEANKIRLDDLCRE
                     VLGAFNATTERQVREAIEVLRRDYHAPVISESGKSGRWLAADVAELQACINEMQNRHN
                     HLGEVIRSLRQDQVPARSPKFDQTTQQSLWS"
     gene            4122..5051
                     /locus_tag="PKL11_06330"
     CDS             4122..5051
                     /locus_tag="PKL11_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13257.1"
                     /translation="MASKRIIDSALFEDDFVGGLDFFGRYLWIGLFSAVADDQGRFLD
                     NAAIIRARVFPFDAVDDAQVEAVLVQLAEAGKINRYQADGKRLVQINHWWVYQQPSWA
                     LPSRFPAPEGWVDRAKYHTKGNKIIVVNWDSEGGYVEDVSSLLPSALPSALPSALCSG
                     LPSPIEEEKREEEIKEDVKQEQEQKTRAPAAAAAARIGYQTDAMILRAWTMATDMPAI
                     PPGDLSKVLPVLESVSARYTSAEDFAAALKPYYQAWLSRRTADGRAYSRTNLGWIDWF
                     AAGEIPPVRGDNRKKQGNQPSAAELAAALGGGV"
     assembly_gap    5053..5152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5153..5959
                     /locus_tag="PKL11_06335"
     CDS             <5153..5959
                     /locus_tag="PKL11_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNZ13258.1"
                     /translation="VSGWYSGELRSAPCPVCCKGQMATYVLHNCGLTGADLDVSLANF
                     KVDGPLLAEKAAALTVCKQLLGQGLAVAGFVTLVGDYGVGKSHLLKGLVNGFRQIGVM
                     AKYSTMSDLLSDIRERFGDEHGAREAEAVIDLYRRARILAIDEVDRINLTGWAKETIF
                     RLLNSRYEESDRLLTVMATNVEPVDLPAELGYLGSRMNGGQIIRVGGPDMRPAVALRR
                     GPLTHAAASAAAAEAAEPETEMVFENAAEAAEKLKALAQQMNVGDHHETH"
     gene            5946..6176
                     /locus_tag="PKL11_06340"
     CDS             5946..6176
                     /locus_tag="PKL11_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13259.1"
                     /translation="MKPTNQKRLEAVYRFIVQYKRDNGGIAPTLVEIGAGTGITSKSL
                     VARYLQHLVNTGRIVLQPGVRNIRVPGEVWHV"
     gene            6169..6648
                     /locus_tag="PKL11_06345"
     CDS             6169..6648
                     /locus_tag="PKL11_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13260.1"
                     /translation="MSKGCAIAGTVLFFVLVIGLCIVLPLMNVFTHLPPVGVAAETTH
                     PIIGLTAAQTDGLWSLVLGSAILLSVSAIVRSVMRYSGPKEVTGNPGPEIMRPLAGGD
                     VDPWAANVSLGGPGTYVGAKRPAYRLDLVSVTPAQDAIDRATPITEMSVDLRSENRR"
     gene            6645..8423
                     /locus_tag="PKL11_06350"
     CDS             6645..8423
                     /locus_tag="PKL11_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HNZ13261.1"
                     /translation="MKTELTITLMILLAVLLAGCVGYEQPTTPNWGLVMTPMPDIYRD
                     ADQAAATLTAAGMQMTATSQAAQMTQQAAAFEATATAEAYQVQATRQAWEATATAQAY
                     QSTSTARVQSTQQALVVQSTQQSLAVQATQQSLDLGRKQMTNVVVAVTPLALLWLVVV
                     YGMVVAWRLLKVRPIRRDPNGDAPILDIEGRILYDPDRNPVPALVAGREPSAPMLVDQ
                     ALQARVTATDQAVDGLRAAGHRYDSAPIAVETEQSGNQVVDAQWRDVDTGWTGRGHSL
                     LLGMGAQGAIPFVPDRTPHLLVAGTTGSGKTMGLLRPLAAMALAQGWRVMVINPAGAD
                     FVPLAAHPNCRTVDAAGAADALEWAANEVTRRSQLLARVGVSTWAGLPADERPERLML
                     AVDELVALAWGREPSVAARIWASAIMITSQGRKLGVCLAAATTDPTNRTLGRPGLTVR
                     DNCTVAAFRVRDEAVSRAALGIGGAEGLGQWRFMARMAGQVVTGAAFHPNDDELKAFL
                     QRRMAPALNQVEIPQRLEAGMPAETRLKIIDLKRRGVSINGICRALYGYTGGQAYEDV
                     KAVLDDAAITTTITTATTGHNAESEA"
     gene            8429..9139
                     /locus_tag="PKL11_06355"
     CDS             8429..9139
                     /locus_tag="PKL11_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13262.1"
                     /translation="MNRITAFLRGWSQLTQVLEATLIDNVAATIPWMAPIIPAWFAYE
                     NMTVVLGMPQAISLVGAAVIEFLGLATVSTTFKLWQYNDSKRKTDQVAPVQIAIGTTI
                     FYLLIVLTVNVILDSSPITSRIAKALLSLLSVAGAVVLAIRAQHSRRLESIELERQER
                     RELRQEIRRERLAESYRQDTGNFPETIDRIGDWRKLTIQEQEAVASMTTTEITQKYPI
                     TERTARLWREKSRNNNRH"
     gene            9233..9931
                     /locus_tag="PKL11_06360"
     CDS             9233..9931
                     /locus_tag="PKL11_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13263.1"
                     /translation="MDNKNHRFFYLMQALAAGVSTRAELAWMVCQNFDLAYPSGSVTR
                     LISRIKDMGLVVTEHVRLFRSGSFELIRLSQQGRQWCLVNGVGGQPGRDGEWDCLRKH
                     HAGDICPRHTAGLLLFAHQARKRNYEIELLPSMGCADWQPDACVDDGHPVFVEFETRA
                     RLEKAQAGKWAKAQRYANLNGGSLGIVAIYPYAREKLAEWAPDALVTDLQSLCRDQQR
                     GLWYTGQDDQAMCL"
     gene            10436..>10907
                     /locus_tag="PKL11_06365"
     CDS             10436..>10907
                     /locus_tag="PKL11_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13264.1"
                     /translation="MTKSKISQRSSLGHQMMTALQSVFKPGKSRHEDKKHGRPYIRGI
                     DTMETMTQDVFAFARYVKANWPGTRQLNQVTPEMTQQYIAELMLRELTGGYIGRVSAT
                     LRKLDIACRRIRIFPQDAPPLLPYASQGGVGGFHSTPSTKAYAPEDAERIITHIA"
BASE COUNT         2318 a         2794 c         3435 g         2160 t
ORIGIN      
        1 aggttgatgg tggcgatgac ggaggtgaag ccgatggcga cgacgatgga tagttggtgt
       61 ttggggaggg tgagggcgta ggattggcag aagaagactg cgccggcgag ataaacggcc
      121 caggcccagc ggcggcggat ggcgcggtgg gcgttatgta gttgaccagg gcggctgttg
      181 acgatagcgg cgcgggcgtc tggagggagg ctaatccaca gtttgctcca gcggattagc
      241 tgggcgacgc ttggctttac gttcctggtc ggctcggtac tgggcatagt ttaggaggtc
      301 ctccctggtg tgttcatcgg cttgcataaa gatttcgatc cactcggcaa gagtgggggg
      361 agcgtctgac tcaacagacg gcaggatgcc ggcagctcga aaaacttcat ctggtggcag
      421 gtgaagcgcc ccggcaatac ttttgagaac gtcggggcca ggcttacggg tgccattgat
      481 caggtggctt atggctcccc tagaaacacc agagaggcga gatagttcac tttggttcat
      541 atctcgccgg gtaagttggt caagtagcca atctgaaaac atttcgttca cagttgacac
      601 ataatagccg cttttgacgc gtcattggta atcagtaaat agtctattga caacattggt
      661 ggaaagttgt atactaatga cgcaccataa aatacagatg acgcagtttg gaggaacgat
      721 ggctgacgat tacatggtca ggatgaatga tttggaagcg gcagaaaaac tcaaggtgat
      781 ggcgaagatg gacaagcgca caatcgggga tcagaacgct tggattatcc accagatgtg
      841 ggcgctgcga actggcctac ctgctgccat gatgccaatt atccctccgg tgattgtgcc
      901 cgatatcaca tcaggagaag ccaagtaaat gggccagggt accactgttc cgcgctgctc
      961 gaaatgcggg cgggtgctga aaagccccga gagcattgcc cgaggaatcg ggccagaatg
     1021 cgcaggcttg cgcgggtatg cccggtcaaa caagttgggc atccggcagg gccggcgggg
     1081 ggcgtatgcg gcgggagact cttcttctgg gagcggggaa ccggctgtac ctgtggtgca
     1141 tgatgacctc catgaagaga gtgtagccga tggcgctgag gatggtgaaa aatgagcgat
     1201 aaaagcacga aaaatgtaca gaaacggaag gcgctcaatg tcatcatcac gcgccggttt
     1261 attccggtgg aagatgggga agagcggtgg cggtatgcca tcagcgagat tttacgcata
     1321 agtctccggt acatgcggca gaacggcatc ccgctgccca caacccatga aacctatccg
     1381 gacgtgtctg taaatgcagc ggacccagag gtggtggaat catgatgcct gagaagattg
     1441 ccagcctgtg tctggtctta tttgtgtttg ggctgatcta taacgtcctg atcgacgccg
     1501 ccgattggcc caggtatctg gttgggctca ccggtttgcc cgatgctgtg cgtgccggca
     1561 tcatgtcggc cggggcaggc tggggtattg agctcgagcg gtggaggttt gcttggagca
     1621 cactggcggc gtttgcctcc caagtggcgt caatggttat cgggactgtg gcgcgcgatg
     1681 tgcgcgtccg tcaaactggt tatctgtgtc tgcttgggga ggttttgact gatgagcggt
     1741 gatcgggcac ggccgtggcc gacaaaagct gcctgggccc gtgatgatgc ggtccagtca
     1801 ctggccgagg cagtgcgaat gctggagcct atggtcgccg gtgagcgcca gatcagcgag
     1861 gcagagatta ttcgacggac tgcccgggca ctggtcgccg ttgagcgggc aatccggatc
     1921 ctggcgcagg cgggagcgca ggtcaagatt gattaactct ggaggcaggt ttttgatgac
     1981 tgattgaact ttacagccga ggtgcaaaac gaaaccggct cgttgcagcg agccggagaa
     2041 aggaggtgag ggtttggatc acgctcgcct ccagtatagc agattttgga ggtttctaca
     2101 atgaccgatc ttaatttatc tgacctgccc cctgaactga tggatctcta ccgacaggca
     2161 gccgttgatc aaagcatgcg gcagcatgag gcgcaagaac gagacgatgc aaatgaggca
     2221 gcccgcctga accgtgagcg ccagacgctg gagcagctga tcgctaacga ccagctgcca
     2281 gaagcagtcc gcccatatgt gacgtttgat tggcggacga gtacatcgga ttacaggccg
     2341 tttagcctgc tggtttcact gcccgatatt gcgccaatta tggtgcactg ggtctggggt
     2401 ggaacgacta atggcccgca accggttagc gttaagtact cgacgccaat ggacatcgag
     2461 gatgatgaag acggcacccc gacctattcg ggttattacc agatcacgga catctttgcc
     2521 gaggcactat acaacgccat ccactatgcg ccttcgatga aaatgcacct caatcgaaag
     2581 acagccgaaa aagcccagcg cgaaaaccag gaacaggctg aagtagatgc tgaggcctct
     2641 caggaagagc atgatgaaaa aatcagggat ctgctgatgg ataatagcta tgatccaacg
     2701 cggggagatg ttggcaacct gctgaaaatg attaattgcc gtctgactgc catcggcatg
     2761 gtcttgcaag atattgcgat tgcccaacgg ggcgaaaaat gaaaccgaaa ttgagcatga
     2821 accaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtaa agccaaaacg
     2941 ttatggctgg ccgactggct ggtcatcctg ctgacggtgg tctgcgggct ggcggttggc
     3001 tgggcgattg tgaccggcaa ctgggggatg ctggtggttg aggtgatcac ctacgccatt
     3061 ggcggaggga tttattcgga atatcgggcg gtgtcacgat gaacccgaac acacccatcc
     3121 tccggcagtt ctcgtttgat gcgctgcgca tctgcggcga atgccatgag cagtttgtgc
     3181 tgggtgtgga tggcgtggtc atggaggggc gtgacctctg cgacaagtgc gccggcgtaa
     3241 cccgcacggt tccgggcggg catgtaattt tgatcaacga caaaccaact gtggaggtta
     3301 aataatgctg ccttctttga tgaccgacat gcagattgac gaagcctggg atatgtacct
     3361 ggcttacctg ggtaatcagg cggaacgggg ctaccggctc tgtgacgggt gccagcagtg
     3421 gtacaacgtc cggcacatgc gccagtacgc cgggggctgg ctgtgcacct gctgtgccag
     3481 caaatatgat ccaacgattg ccccggaaga tacccacccg ccagtcgcac agatctgcgg
     3541 ctactgcggc gatgcccacg acagcctgac ggccaagtta gacggtgtgg actgctgcga
     3601 gagctgtatg cagattgtga ttgacgcctc gaccccctgt tgatttcatc cttttctcct
     3661 ctctaagggc tgccggcttg acccaccggc agcccgctac cccgaaaggc ggccccatga
     3721 caaccacccc attctatgac gacctgattg ctgcgatgcc ggcaggactt gaacggagag
     3781 tgtttgaagc actccaagtg catgttggcg aggccaataa aatccggctg gatgacctgt
     3841 gccgggaagt tcttggcgct ttcaatgcca caactgagcg ccaggtgcgc gaagcgattg
     3901 aagtgctgcg gcgtgattac catgcgccgg ttatctctga gagcggcaaa tccgggcgtt
     3961 ggctggccgc tgatgtagcc gagctgcagg cctgcatcaa tgaaatgcag aaccggcaca
     4021 atcatctggg tgaagtcatc cgcagcctgc gccaggatca ggtgccggcc agatcgccga
     4081 agttcgacca gaccactcag caatcactct ggagttgagt tatggcaagt aaacggatta
     4141 tcgactctgc actgttcgag gacgactttg taggcgggct ggacttcttt ggccgttacc
     4201 tgtggattgg cctgttctcg gcggtggcgg atgaccaggg gcggttcctg gataatgcgg
     4261 ccatcatccg ggcgcgggtg tttccgtttg atgccgtgga cgatgcccag gtggaagcgg
     4321 tactggtgca gctggccgaa gctggaaaga tcaaccgtta ccaggcagat ggaaagcgcc
     4381 tggtgcagat caatcactgg tgggtctatc aacagcccag ctgggcatta cccagccgct
     4441 ttccagcccc ggaaggttgg gttgaccggg caaaatatca cacgaagggc aacaagatca
     4501 tcgttgtgaa ttgggacagt gaaggcggct atgttgagga tgtatccagc ctgctaccta
     4561 gtgcgctacc tagtgcgcta cctagtgcgc tatgtagtgg cctacctagc ccgatagagg
     4621 aggagaagag ggaagaggag ataaaagagg atgttaagca ggagcaggag cagaagactc
     4681 gcgcacctgc ggctgctgcg gcggctcgaa ttggctatca gaccgatgcg atgattttga
     4741 gggcctggac aatggccacc gacatgccag cgatcccacc gggagacctg tcaaaggtcc
     4801 tgccggtgtt ggaatctgtc tcggcgcggt acacatcggc cgaagacttt gcggccgcac
     4861 tcaaacccta ttaccaggcc tggctgtccc ggcgcactgc cgatggccgc gcctattccc
     4921 ggacaaacct cggctggatt gactggtttg ccgcggggga gattccgcct gtcaggggcg
     4981 ataaccgcaa aaagcagggc aaccagccca gcgcggctga actggcggcg gctttaggag
     5041 gtggtgtatg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtctcagg
     5161 ctggtacagc ggcgaattgc gcagtgcacc ctgtccggtg tgctgcaagg ggcagatggc
     5221 gacctatgtg ctgcacaact gtgggctgac cggcgccgat ctggatgtga gcctggccaa
     5281 cttcaaggtt gatgggcctt tgctggctga gaaagccgca gccctgacag tctgcaagca
     5341 gctgctgggc cagggcctgg cggtggcggg gtttgtcacc ctggtgggtg attacggcgt
     5401 cgggaagtcg cacctgctca aagggctggt gaacggcttc cggcagatcg gcgtgatggc
     5461 caagtattcc accatgagcg acctgctctc cgacattcgg gagcggtttg gggatgagca
     5521 tggggcgcgg gaagccgagg ccgtgattga cctctaccgc cgggcgcgta tcctggcgat
     5581 tgacgaagtg gatcggatca acttgaccgg ctgggcgaag gaaacgattt tccggctgct
     5641 gaacagccgt tacgaggaat cggaccggct gctaaccgtc atggcgacca acgtcgaacc
     5701 ggttgatctg ccggccgaac tgggctacct gggcagccgg atgaacggcg gccagatcat
     5761 ccgtgtgggt gggccggaca tgcgcccggc tgtggcgctg cgccgggggc cgctgaccca
     5821 tgcggcggcg agcgcggcgg cggctgaagc ggctgagcct gaaaccgaga tggtattcga
     5881 gaatgcggcc gaagcggctg aaaagctgaa ggctttggcc cagcagatga acgttggaga
     5941 tcaccatgaa acccactaat caaaaacggc tggaggcagt gtaccgcttt atcgtgcagt
     6001 ataagcgtga taacggcggc attgcgccaa cactggttga aatcggggct ggaacgggca
     6061 tcaccagcaa atcgctagtg gcgcgctatt tgcagcacct ggtcaatact ggccggattg
     6121 tactccagcc gggcgtgcgc aatatccggg tgcctgggga ggtgtggcat gtctaaggga
     6181 tgtgcgattg caggtacggt tctgtttttc gtcctggtga tcgggctgtg catcgtcctg
     6241 ccgctgatga atgtgttcac acacctgcca ccggtcggtg tggcggcaga gaccacgcat
     6301 ccgatcattg ggctgacggc ggcccagacc gatggccttt ggagcctggt gctggggtcg
     6361 gcgatcctgt tatcggtatc ggcgatcgtg cggagcgtga tgcgttacag cgggccaaaa
     6421 gaagttactg gcaaccctgg gccggagatc atgcggccac tggcgggtgg agacgttgac
     6481 ccctgggcgg ctaatgtatc actgggcggg cctgggacgt atgtcggggc aaagcggcca
     6541 gcgtaccgcc ttgacctggt atccgtaacc ccggcgcagg acgccattga tcgggcgacc
     6601 ccgatcacgg agatgagcgt ggacctacgc tcggagaatc ggcgatgaaa acagaactaa
     6661 ctattaccct gatgatcttg ctggctgtcc tgctggcggg atgcgtggga tacgagcagc
     6721 ctacaacccc caactggggg ctggtgatga caccgatgcc ggatatctac cgggatgctg
     6781 atcaggctgc cgcaacactg acagcggccg ggatgcagat gacagcgacc agccaggcgg
     6841 cccagatgac ccagcaagct gcggcctttg aggccaccgc tacggctgaa gcctaccagg
     6901 tgcaggccac ccggcaggcc tgggaagcca cggcgacagc tcaggcatat caatccacct
     6961 caacggcccg ggtacagagc acccagcagg ccctggtggt gcagagtacg cagcagtccc
     7021 tggctgtgca ggccacccag caatcgctgg acctggggcg caagcagatg accaatgtgg
     7081 ttgtcgccgt caccccgctg gccttgctgt ggctggtggt ggtgtatgga atggtggtcg
     7141 cctggcggct gctcaaagtg cgcccgatcc ggcgagaccc caacggggat gcccccatcc
     7201 tggatattga ggggcgaatc ctgtacgatc ctgaccgcaa tcccgtgccg gcactggtgg
     7261 cagggcgtga gcccagcgca ccgatgctgg ttgaccaggc gctccaggcg cgggtgacgg
     7321 caactgacca ggctgtggat ggcctgcggg cggcggggca ccggtatgac agcgctccga
     7381 tcgcggtaga gactgagcag tcaggcaacc aggttgttga tgcccagtgg cgggatgtgg
     7441 ataccggttg gacggggcga gggcattccc tgctgctggg catgggcgca cagggagcaa
     7501 tcccgtttgt cccagaccgc accccacacc tgctggtggc cgggaccacc ggcagcggca
     7561 agacaatggg cctgctgcgg cctctggcgg caatggcgct ggcccagggc tggcgggtga
     7621 tggtcatcaa cccggcagga gcggattttg tcccgctggc ggctcacccg aactgccgga
     7681 cggttgacgc tgccggggcg gctgatgccc tggaatgggc cgctaacgag gtcacgcggc
     7741 gcagtcaact gctggccagg gtcggcgtat ccacctgggc agggctgccg gcagatgagc
     7801 ggccagagcg tttgatgctg gctgtggatg aactggttgc cctggcatgg ggccgggagc
     7861 ccagtgtggc ggctcggatt tgggccagtg cgatcatgat taccagccag ggccgcaagc
     7921 tcggcgtctg cctggcagcg gcgaccacag acccgaccaa ccgcacactg gggcggcctg
     7981 gtctgaccgt gcgcgataac tgcaccgtag cggcgttccg tgtccgtgat gaagccgtct
     8041 cacgagctgc gctgggaatc ggcggggccg aagggcttgg ccagtggcgg ttcatggccc
     8101 gcatggcagg ccaggtcgtc accggggcgg cgtttcaccc gaatgatgac gaactcaaag
     8161 cattccttca acggcggatg gctccagcat taaaccaggt tgagatcccg caacggctgg
     8221 aggctggcat gccggccgag acacggctga aaatcattga cctcaaacgg cgaggggtga
     8281 gcatcaacgg aatctgccgg gcgctgtatg gatatacggg cgggcaagcc tacgaggatg
     8341 tgaaggcggt gctagatgat gccgctatta ctactactat tacgaccgct acgaccgggc
     8401 ataatgctga aagtgaggcg tagtcgtaat gaatcgcatc acagcttttc tccgtggttg
     8461 gtcgcagctc actcaggttc tggaagccac gctgattgat aacgtggctg ccacaatccc
     8521 ctggatggct ccaatcatcc cggcctggtt cgcatatgag aacatgacgg ttgtgctggg
     8581 aatgcctcag gcgatcagcc tggttggcgc tgccgttatt gagtttctgg gattggccac
     8641 tgtttcgaca acattcaagc tgtggcagta caacgactcg aagcgcaaga ctgaccaggt
     8701 agcccctgtg cagatagcga ttgggacaac catcttttat cttcttatcg ttttgacagt
     8761 caatgtcatt cttgatagtt ccccaataac ttctcggata gcaaaagctc tcctttctct
     8821 gctcagcgtg gccggggcag tggtattagc catccgggca caacattccc gccggttgga
     8881 gagtattgaa ttggaacggc aagaacggcg agagttgcgg caagaaattc gccgggagcg
     8941 gctggcggaa agttaccggc aagataccgg aaactttccg gaaacaattg atcgaatcgg
     9001 agactggaga aaacttacta tccaggaaca ggaagccgtt gcttctatga ccacaacgga
     9061 gattacacaa aaatacccga tcacagaacg aactgcccgt ttatggcgcg agaagtccag
     9121 aaacaataac cggcattaaa ttgatccact taatcccacc gggcataagt tgcaactggc
     9181 gcagaataat tttttggcct gataaccctg ccgcatatga gtaataaata tcatggataa
     9241 taaaaatcac cgatttttct acctgatgca ggcgctggct gccggggtga gcacgcgggc
     9301 tgaacttgcc tggatggttt gccagaactt tgacctggct tatcccagtg gatcggtcac
     9361 gcggctgatc agcaggatca aggacatggg gctggtcgta acggagcacg tcaggctgtt
     9421 ccggtcgggc agctttgaac tcattcggct gagccaacag gggcggcagt ggtgcctggt
     9481 caatggcgtt ggtgggcagc ctgggcgtga tggcgagtgg gattgtctgc gtaagcatca
     9541 tgccggcgat atctgtccgc gccatacggc aggcctgctg ctgtttgccc atcaggcccg
     9601 gaagcggaat tacgagatcg aattattgcc ctctatgggt tgcgcggact ggcagccgga
     9661 tgcctgcgtt gacgatgggc atcccgtatt tgttgagttt gaaaccaggg cgcggctgga
     9721 gaaggctcag gctggcaagt gggcaaaggc gcagcggtat gccaatctca acgggggcag
     9781 cctgggcatt gtggcgatct atccgtatgc ccgtgaaaag ctggccgagt gggcgccaga
     9841 tgcgctggtg actgacctcc aaagcctgtg ccgggatcag cagcggggtt tatggtatac
     9901 ggggcaggat gatcaagcca tgtgcctcta ggtgtaaaga taacacccca ccttgcattt
     9961 tggtaaggtg gggtgttttg aactttatgc agcgacggga tgtcgtataa tcaatttaac
    10021 tcatatgctg tcctgaacgg cataggttac acgttagctg gttgcaagtt gaccttagaa
    10081 taccaataaa aaagtcctaa tcacaaggat ctgccgcgat gcggcagaac ggacgatctc
    10141 cggcaagcct ggagatagcc acacacaagg agattttgcc caactgggca caactcgacc
    10201 aggtacgcct ggctgagcgt gatgcgagat ttttggcgtt gttctctcga catcgcgggc
    10261 ctccttccaa acaacacccc ctccatgcga gggcgctccg agaggagcgt gtggccgtaa
    10321 ggccatccca acgaggcgtt tttattcaca ggaaacaggc ggaaagccca ttcggcagcc
    10381 aaaaagctgc cagctttcgc gtttccgcat tctgctaact gtcgcgaggt aaaccatgac
    10441 aaaatcaaaa atcagccagc gtagttcgct ggggcatcag atgatgaccg ccctgcaatc
    10501 tgttttcaag cctggaaaaa gccggcatga ggacaaaaag catggccgcc catatatccg
    10561 cggtattgac acgatggaga cgatgaccca ggatgtcttt gcctttgcgc gctacgtaaa
    10621 agcaaactgg ccgggcacca ggcagttgaa ccaggtgacg ccagagatga cccagcagta
    10681 tattgctgaa ttgatgctcc gtgagctgac tggtggctac attggcaggg tgagtgccac
    10741 cctgcgcaag ctggacatcg cctgccggcg catacgcatc tttccccagg atgccccacc
    10801 actgctgccc tatgccagtc agggcggcgt gggcggcttt cactcgacac ccagcacgaa
    10861 ggcatatgct ccagaggatg ctgagcgcat tatcacccac attgccc
//
LOCUS       DAOPVS010000058        10811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6165_length_10811_cov_64.655634, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000058 DAOPVS010000000
VERSION     DAOPVS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6165_length_10811_cov_64.655634"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PKL11_06370"
     CDS             complement(<1..1124)
                     /locus_tag="PKL11_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ13265.1"
                     /translation="MDTMVFGHKLVILLSASGLITGAGLLLLGILWLISRLKVWRLDV
                     VLFHAGAGIPAFTLAGLLLLMIDNFTYTVLNFGIVTSERLLRGLYAILFVGLLYLSYR
                     QVGKWVRRLNARKPRGLWVISAGLVIVAALVTGGLSLSNTSSATAIKTGTTGQRPNIL
                     IIGSDGLSADHMSLYGYELETTPFLSRLAEDSLWAENAFSNSSNTSGSLISMLASKPP
                     AATRVLYPPDILSGINAYQHLPGLLRNAGYTTVQLSIAHYADAYAFNVQNGFDMVNGQ
                     ASLEHSQIYGLIRRVLPGDVAYFISGTADRLFDRLLHIFFVRAMPNPYKTVTTGEVEV
                     TDRQRVDQIIQLVTTTDQPVFIHAHLMGTHGPRYEEITEPV"
     gene            complement(1399..1860)
                     /locus_tag="PKL11_06375"
     CDS             complement(1399..1860)
                     /locus_tag="PKL11_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ13266.1"
                     /translation="MKKQQIMDKINQGWQDLEAAYAGLTEVQMTLPGVTGEWAVKDIL
                     AHVSWWEEESLKHLPDILAGKQPPRYSATYGGIDAFNALMTQQWRELPLPEVLRKLDE
                     THHRLLAYLDSVPEAHFKTETRFRRRLGWDSYKHYPVHTQAIQEWRARVML"
     gene            complement(1876..3705)
                     /locus_tag="PKL11_06380"
     CDS             complement(1876..3705)
                     /locus_tag="PKL11_06380"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNZ13267.1"
                     /translation="MGSTIWIAGVVIVLLVLAGWLASLIWGKPWSVQGLFLRTFAKFV
                     FKGPELLTMLGFLEKFGLHGHNARLSDASEAFQDKTYRFVRRDLGILRSYNRQRMPRG
                     VQISADVMAAFLDDIVEGERFRYHDYPVNQMFGIQSEMPNFMMTIHPVISKLEARNYV
                     RRLNRFGVKFDQVLEGLRIREAKGCIPPRFVIRRVLDEMIAFIGAPARENPLYTVFAE
                     KLDKLNNVKVADQQQILAEAAAAIENTVYPAYQKFIDYFTALEQKATEDDGVWKLPDG
                     DAYYAYCLRSNTTTNYTPAEVHEIGLREVERIEAEMRAIFERLGYANVENPTRKLNEL
                     GKEERFLYANTDEGRAACLGEYQHILDEMNAALDPYFAVRPKAILKVERVPEFREKTS
                     AGAYYQMGALGGGRPGIFFANLRDMNEIHKFGMKTLSYHEGIPGHHFQISIAQELKSV
                     PIFRRMLPFTAYAEGWALYAEKLAREIGMYQDDPYSELGCLDSELFRAVRLVVDTGIH
                     YKRWTREQAIEYMSAHAAQEQESVISEIERYIVMPGQACAYKIGMIKILELRERAQKA
                     LGSAFDLREFHDVILKNGSLPLSILDEIVQAYIRQKTAEHSQV"
     gene            complement(3791..5713)
                     /locus_tag="PKL11_06385"
     CDS             complement(3791..5713)
                     /locus_tag="PKL11_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13268.1"
                     /translation="MGFHGGWRGLINSPDDKPQITWGLLKRVLKYARPYRWALIGMLL
                     LILAHTGLMLLTPLILRDLIDKTIPAGDVRRLIWLALALLLIPAVNGVINVVQRRLNV
                     RVGEGVIYDLRMALYANLQRMSLRFFTNTKVGELMSRLNNDVVGAQNAISSTIVGIIT
                     QIVQAVAVLTVMLTLEWRLTLISVVILPLFILAARLLGNRLRDMARKQMEANAQMNAM
                     MNETLNIGGALLVKLFGRKQLEVDRFGQRAAQVRDLGVQRALIGSVFMAIIGLISAVG
                     SALVYGLGGYFVIQGAFTIGTIVAFGSYLGSLYGALQGLSNAPVEFATSMVSFERVFE
                     VIDLPVDIPEKADAVALRDVRGEIIFDQVSFQYESGDEKLLSDVHRYGSMDNVTAVLS
                     GAKPNDDEGEEPERHSQARSTALEEVSFRVQPGQLVALVGPSGAGKTTLTYLIPRLYD
                     PSTGIIRIDGHDLRDITLESLSAQIGMVTQETYLFHDTIRTNLQYARLNASQEELEAA
                     CRVANIHPFIQDLPDGYDTIVGERGYRLSGGEKQRIALARVILKDPKILVLDEATSSL
                     DSESEALIQDALGKVMAQRTSIVIAHRLSTILAADLILVIDRGRIVESGNHTSLLAQN
                     GLYASLYQTQFRKDRA"
     gene            complement(5826..6611)
                     /locus_tag="PKL11_06390"
     CDS             complement(5826..6611)
                     /locus_tag="PKL11_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNZ13269.1"
                     /translation="MLKLSVGQASFQVTQLEANVEVIRARLAEADADGTDILVLPELA
                     NSGYTFGSREEAAASAEAVDGGPLCELLQDWSGAGRLVVSGLCERAGETLYNSAVVYA
                     EGRLLAVYRKLHLFARESEWFKPGDAPAPVFDFRGYRLGVMICFDWVFPEMARSLALR
                     GAQVILHPANLVLPYCQAAMVTRSIENRVFTATANRCGQDRELHFSGLSQVTAPNGTL
                     LAQAGADFMGVVGAAIDPSKADNKWITRQNHLFEDRRPDLYGL"
     gene            complement(6630..7388)
                     /locus_tag="PKL11_06395"
     CDS             complement(6630..7388)
                     /locus_tag="PKL11_06395"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ13270.1"
                     /translation="MNAQILPKANSFLHPGGATGCILMHGFSCSPEEMLPLDAFLTEK
                     GYTTLGLRFAGHGTTPRDLKDVRWTDWLANIEDAFHILQGSCQRVVLIGQSMGGILSL
                     TAAAYLPVEAVIALSTPFWVPTASEKVVNRLLTLIQPTIRKRNVQPHPEWGWRREAGY
                     PAYAQFPAKINRQTTLLMKAMRENLGRVKAPVLLIQSKQDELIPKNSLESIQAGLTAA
                     RVTPVWIEGPGHGITLDPQAEKAFQPIDAFLAAL"
     gene            complement(7457..9838)
                     /locus_tag="PKL11_06400"
     CDS             complement(7457..9838)
                     /locus_tag="PKL11_06400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNZ13271.1"
                     /translation="MEILSFEQTESCPPIIVSVHGLVEFALQAGDITPGGFQKRDRAQ
                     QGTWGHKQVQRARPEGYESEVEIAYQVEGADPPLEIRGRIDGLYAHQDPVIIEEIKTT
                     TLSLELVNEEHNRLHWAQAQCYAYMYARQHHLDSICVHLTYYHLDSRKEKRFERIFSM
                     AELEVFFSDLVTPYLSWMRKMRAWQARRNQSIQQLGFPYAEYRSGQRDMAVAVYKTIR
                     ANGRLYVQSPTGVGKTIAALFPAVKALEQGLAAKIFYLTAKTPGRQVAEKTLEDMRQA
                     SLQLISVTLTAKEKICFCPPVNCDPEVCEFARDYFGKVKQALEGIDQHQAYTRPVIEA
                     LAREYQICPFEFSLDLALWADCIICDYNYAFDPRVYLHRFFDFCTEPYIFLVDEAHNL
                     PDRAREMYSAEIDKKNVLELQRALKPHLPDLAQKLNALNKVMLQIRKACQAAGKEALI
                     ENSLPEELLKAIRKFSQAAEDWLALNQAAEFRQDLLTFYFLCLNYLRTAEYFDTYYVS
                     YFERQGASDLKAKLFCLDPAPMLAAPLEHSQSAVFFSATLLPMDYFMRLLTGDARHPR
                     QIFQSPFPIENTCLLIQNQISTKYAQRADSYAEIAATIEAVCNTHVGNYLVFFPSYAY
                     LAAVLEILKDKLPEEQLMVQERAMTEVDREAFLARFSASNTTTLVGLAVMGGIFGEGI
                     DLVGERLIGVVIVGVGVPQLGLERDLIKAYFDQKSGSGFAYAYQYPGFNRVLQATGRV
                     IRTETDRGIIVLIDDRFTHARYRQLFPTHWRGFQVVQNVDEIKDKLTRFWAQD"
     gene            10077..>10811
                     /locus_tag="PKL11_06405"
     CDS             10077..>10811
                     /locus_tag="PKL11_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ13272.1"
                     /translation="MNNILALLQPIRNSVSKTTLRQMSRIIAAMLSMTGRVTMLGLSR
                     WAEKGGSYRTVQRFFHTAIPWATLFWGFFCKHLWDPQETYILAGDESVVTKAGEKTHG
                     LDYFFSGLLKRTVPGLSFFTLSLIGTQRRQSFPVLVRQMVRTTEEKAASQAKKAQKSG
                     ERRKKGRPKGSRNKGKAEVTLNPELLRIQEMVKSLLQTMQGAIRLSYLVMDGHFGNHP
                     AVHMVRACGLHLISKLRHDAALYMPYL"
BASE COUNT         2574 a         3139 c         2591 g         2507 t
ORIGIN      
        1 actggctcgg ttatttcttc ataccttggg ccatgggtcc ccatcaggtg ggcatgaatg
       61 aaaaccggtt gatctgtggt tgtcacaagt tgaatgatct ggtccacgcg ctgccggtcg
      121 gtaacctcaa cctcgccggt tgtgacggtt ttatacgggt tgggcattgc ccggacaaaa
      181 aatatatgca gcagtcggtc aaagagccgg tctgccgtgc ccgatataaa ataggccaca
      241 tcgccaggca gcactcgccg gatcaggccg taaatctggc tgtgttccag ggatgcctga
      301 ccattgacca tgtcaaaacc gttttgcaca ttaaacgcat aggcatcggc gtagtgcgca
      361 atactcaact gcacagtcgt ataaccggcg ttgcgcagca aacccggcag gtgctgatac
      421 gcattgatgc cagaaagaat gtctggtggg tacaaaaccc gcgttgccgc cggcggtttg
      481 gatgccagca ttgaaatcag cgagccgctg gtattggacg aattgctgaa tgcgttttct
      541 gcccataaag aatcttcggc caggcggctc aagaatgggg ttgtttccag ttcatatcca
      601 tacaaagaca tatgatccgc agacaaccca tccgaaccaa tgatgagtat attgggccgc
      661 tgaccagtag tgcctgtttt tattgccgtt gcagagctgg tattcgataa gctcaatcca
      721 cctgtcacca gtgctgccac aatcactaaa cctgctgaaa tcacccaaag ccctcgcggc
      781 ttgcgggcat tcaagcgccg tacccacttc cccacctggc ggtaactcag gtacagcaag
      841 cccacaaaca aaattgcata caatccgcgc agcaggcgct cgctggtcac aattccaaaa
      901 ttcagcacag tataggtgaa gttatcaatc atcaacagca gcagccccgc cagggtgaag
      961 gcaggtattc ccgcaccggc atggaacaga accacgtcca gccgccagac cttcaaacgg
     1021 ctgatgagcc acaggatccc caataacagc aggcccgctc cggtgatcaa gccgctggcg
     1081 gataagagaa taaccagttt atgcccaaaa accattgtgt ccataaatga cggcttggtt
     1141 accataaaca gccattccat gaacccaaat ccaaacccgg taagaatcgc aatttccacc
     1201 aggagccccc agccttttgg tgacggctcg attttcaggc cgttaacttt tgccttgatg
     1261 aataaaatca gaaagctgag gctcttatgg atcaggctcc ctgtctttga ttgtcttgcc
     1321 tgctgcatcg cctgctcccg cgcggatgtg atttccgggc aagtcaggct taatctgact
     1381 tttgccggag ttgccgtatt atagcatcac tcgggcgcgc cactcctgga ttgcctgcgt
     1441 atgcacgggg tagtgtttat aagaatccca ccccagccgc cgcctgaaac gagtctccgt
     1501 cttgaaatgc gcttcaggca cgctgtccag gtatgccagc agccggtgat gggtttcgtc
     1561 cagcttccgc aatacctcgg gcagcggcag ttcacgccac tgctgcgtca tcagcgcatt
     1621 gaaagcatcg atcccgccgt aagtcgccga atatcgcggc ggctgcttac ccgccaggat
     1681 atccggcagg tgcttgagcg attcttcttc ccaccagctc acatgcgcca gaatatcctt
     1741 aactgcccac tccccggtga cacccggcag agtcatttgc acctcggtta atccggcata
     1801 agcggcttca agatcctgcc agccctggtt gattttatcc atgatctgct gttttttcac
     1861 cgcaccatcc tccaatcaga cctggctgtg ctctgctgtt ttctggcgaa tatacgcctg
     1921 cacaatctca tccaggatag acagcggcag gctgccgttc ttcaagatca catcatggaa
     1981 ttctcgcagg tcgaatgctg accccagggc tttctgagcg cgttctcgta gctccaagat
     2041 tttaatcatt ccaatcttgt aagcgcaggc ctgtcccggc atcacaatat aacgttcaat
     2101 ctcgctgatc acgctctctt gttcctgcgc ggcatgggcg ctcatgtatt caatcgcctg
     2161 ttcccgtgtc cagcgtttgt agtggatccc cgtatccact accagccgca ctgcccgaaa
     2221 cagctcagaa tccaggcagc ccagctcgct gtagggatca tcctgataca taccaatttc
     2281 gcgcgccagt ttctcggcat acagcgccca gccttcagca tatgcggtaa agggcagcat
     2341 tctgcggaag atgggaacac tcttcaattc ttgagcaatg gaaatctgaa aatgatgtcc
     2401 tggaatccct tcgtgatagg aaagggtctt cataccaaac ttgtgtatct cattcatatc
     2461 gcgcaagttg gcaaagaaaa tccccggccg cccaccaccc agcgcgccca tttgataata
     2521 agcgccagcc gatgtctttt ctcgaaattc cggcacgcgc tccactttca ggatggcttt
     2581 aggccgcacc gcaaaatagg ggtccagcgc ggcattcatc tcatccagta tgtgctgata
     2641 ttcacccagg caggctgccc gcccctcatc tgtgttggca tagagaaaac gctcttcttt
     2701 acccagttca ttcagttttc gggtagggtt ttcaacgttt gcatatccca gccgctcaaa
     2761 gatggcccgc atctcagcct caatccgctc aacctcgcgc aacccgattt cgtgcacctc
     2821 tgccggggta tagtttgtag tggtattgct gcgcaggcag tacgcataat aggcatcacc
     2881 atcgggcagc ttccatacac catcatcctc ggtggctttt tgttccaatg ctgtaaagta
     2941 atcaataaac ttttggtaag ctggatacac cgtgttttct atcgcagcgg ctgcctcagc
     3001 gagaatctgc tgctgatctg ccaccttcac gttattcagc ttgtccagct tctcggcaaa
     3061 caccgtgtaa agtggattct cgcgggcagg tgcgccaata aaagctatca tctcatctaa
     3121 aacccggcgg attacaaagc gcggcggaat gcagcctttg gcttcccgga tccgcaaccc
     3181 ttccaacacc tggtcaaact taacgccaaa tcgattcagc cgccgcacat aattcctggc
     3241 ttccagcttg ctgatgaccg ggtgaatcgt catcataaag ttgggcattt cagattgaat
     3301 cccaaacatt tgattgactg gataatcgtg gtagcgaaaa cgctcgccct cgacaatatc
     3361 gtccagaaat gccgccatca cgtcagccga gatctgaacc ccgcgcggca tcctttgacg
     3421 gttgtagctg cgcagtattc ccaggtcgcg ccgcacgaat cgatacgtct tatcctgaaa
     3481 tgcttctgaa gcatctgaaa gcctggcatt atgcccgtgc agcccaaact tttccaaaaa
     3541 tcccaacatg gtcagcaact cgggcccctt gaacacaaac ttggcaaatg tgcgcaggaa
     3601 caatccctgc accgaccacg gcttgcccca gatcaggctt gccagccacc ccgccaggac
     3661 cagcagaacg ataaccactc cggcaatcca aatagtgctg cccatgaaga actccttcag
     3721 tcaggtattg tatatattga attgtacagc aaaacatcca aaatcagttt gctgaaagcc
     3781 gttgattgaa ctatgcccta tctttgcgga attgtgtctg atacaggctg gcataaagcc
     3841 cattctgagc tagcagactg gtatgattgc cgctttcaac aatcctgccc cggtcaatca
     3901 ccagaatcaa atcagccgcc agaatggtgc ttaaccggtg cgcaatcaca atgctggtgc
     3961 gctgcgccat cactttgccc agcgcatctt gtatcagggc ttccgattcg ctgtccaggc
     4021 tgctggtggc ttcatccaga acaagaatct tgggatcttt tagaatcacc cgcgccagcg
     4081 ctatgcgctg cttttcgccg ccgctcaagc ggtatccgcg ctcgccaaca atggtgtcat
     4141 acccatcagg caaatcttga ataaacggat ggatgttggc aacccggcag gcggcttcca
     4201 attcttcctg gctggcattc aagcgagcat actgcaaatt ggttcgaatg gtgtcgtgga
     4261 acagataagt ttcctgcgtc accatcccaa tctgagctga aagcgattcc agcgtgatgt
     4321 ctcgcaaatc atgcccatca attcggatga tccctgtgct ggggtcatat aatcggggga
     4381 tgagataggt cagggtggtt ttacccgcac cgctggggcc caccagtgca accagctggc
     4441 cgggttgaac ccggaaactg acttcttcca gtgccgtgct gcgagcctgg ctgtgacgtt
     4501 ccggctcttc gccctcgtca tcattgggct tggctcccga aagcaccgcc gtcacgttat
     4561 ccatgctgcc ataccggtgc acatcgctca gcagcttctc gtcgccgctt tcatattgga
     4621 acgaaacctg gtcaaaaata atttcgccgc gcacatctcg cagtgccacc gcatcagcct
     4681 tttctggaat gtccaccggc aggtcaatta cttcaaacac gcgttcaaag ctgaccattg
     4741 aggttgcaaa ttccactggc gcattggata agccctgcag cgcgccgtac agactgccca
     4801 ggtaagaccc aaaggccaca attgtgccaa tcgtaaaggc accctggatg acgaaatatc
     4861 cgcccagccc atacaccagc gccgacccca ctgcgctgat caggccaata attgccataa
     4921 atacagaccc aatcaatgcc cgctgcacgc ccaggtcgcg cacctgtgcc gcgcgttgcc
     4981 caaaccgatc cacttccagt tgcttgcgcc caaacagctt gaccagcagc gcaccgccaa
     5041 tgttgagtgt ctcattcatc atcgcattca tctgcgcgtt ggcttccatc tgtttgcgcg
     5101 ccatatctcg tagccggttg cctaaaagcc gcgccgccag aataaacaac ggcagaatca
     5161 ccacactgat cagggttaat cgccactcca gggttagcat cacggtcaag accgctacag
     5221 cctgaactat ttgcgtgatg attcccacaa tcgtgctgct gatggcattt tgagccccca
     5281 ccacatcgtt gttcaggcgg ctcatcagct cgcccacctt ggtattggtg aaaaaccgca
     5341 gtgacatccg ctgcaggttg gcatacagcg ccatgcgcaa atcgtagatc acgccttcgc
     5401 ccacgcgcac attcagccgc cgttgaacca cattgatgac cccattcacc gctggaataa
     5461 gcagcagcgc cagcgccagc cagattaacc ggcgcacatc ccccgccggg attgtcttgt
     5521 caatcaagtc tcgtagtatc aacggcgtca gcagcatcaa gcccgtatgg gccaaaataa
     5581 gcagcaacat gccaataagt gcccaccgat aagggcgggc atacttcaaa acccgtttga
     5641 gcagccccca ggtaatttga ggtttatcat cgggcgaatt gatcagccct cgccagcccc
     5701 catgaaatcc cattgtgcac ctcttgaatg atgatcttgg aagtattatc ccacactttg
     5761 cggcggtttc ctccccaatc tcaacccgtc aggcgctctt atgtaagcta tctctcaacc
     5821 cgggttcaca acccatacag gtctggtcgc cggtcctcaa atagatgatt ttgccgtgtt
     5881 atccacttat tatccgcctt gctcgggtca atggccgcgc caaccacccc cataaaatcc
     5941 gccccggctt gcgccagcag ggttccattc ggcgccgtga cctgtgagag ccctgaaaaa
     6001 tgcagttcgc ggtcttgccc gcagcgatta gctgtggcag tgaaaacccg attctctatc
     6061 gagcgtgtga ccatcgccgc ctggcagtaa gggagcacca gatttgctgg atgcagaatc
     6121 acttgtgctc cgcgcagcgc cagcgagcgc gccatttcgg gaaaaaccca gtcaaaacag
     6181 atcatcaccc ccagccggta tcctcggaag tcaaacacgg gtgccggcgc gtcgcccggc
     6241 ttgaaccact cgctttcgcg ggcaaagagg tgcagcttgc ggtacaccgc cagcaaacgc
     6301 ccctctgcgt aaaccacagc cgaattatag agtgtctcgc cagcgcgctc gcataatcca
     6361 ctgaccacca gccgccccgc cccggaccaa tcctgtagca gctcgcaaag cggcccgccg
     6421 tcaaccgctt ccgcgctggc agcggcttct tcccggctgc caaacgtgta ccccgagtta
     6481 gccagctcgg gcagcaccag aatatccgtg ccatccgcat ctgcttctgc cagccgggct
     6541 cggattacct cgacgttggc ttcaagctgc gtcacctgaa aactggcttg cccaacagat
     6601 agcttaagca ccttgtccgc ctccgcaaat tacaatgctg ctaagaatgc gtcaataggc
     6661 tgaaaagctt tttctgcttg agggtccagc gtgataccat gcccggggcc ttcgatccac
     6721 accggggtca ctctggccgc ggttaacccg gcctgaatac tttccaggct gttcttgggg
     6781 attagctcgt cttgtttaga ctggatcaac agtaccggag ctttcaccct gcccaaattt
     6841 tcacgcatgg ccttcatcag cagtgtagtc tggcggttaa ttttagccgg gaactgcgca
     6901 taagccggat aaccggcctc gcgccgccaa ccccactccg ggtggggctg cacatttctt
     6961 ttcctgatgg tcggctgaat caacgtcaaa agccgattca ccactttctc cgatgctgtg
     7021 ggtacccaga acggtgttga gagcgcaatg acagcctcga ccggcaaata agccgctgcc
     7081 gtcaggctaa gtattccccc catagactgc ccaatcaaga ccacccgctg acagctcccc
     7141 tgaagtatat ggaaggcatc ctcaatattc gccagccagt cggtccagcg gacatctttc
     7201 agatccctgg gcgtggtccc gtgccccgca aaacgcagcc ccagcgtggt atagcccttc
     7261 tcggttagaa aggcatccag cggcagcatc tcctcaggtg aacagctaaa accgtgcatc
     7321 agaatgcagc ctgtagcgcc gccggggtgt aagaacgaat ttgctttggg caggatttgg
     7381 gcgttcatca tcagtccttg tgattaatcg ggatatgctt caatcatacc ccgttttaga
     7441 aaaaaggaaa caggtattaa tcctgcgccc agaatcgggt caacttatct ttaatctcat
     7501 ccacattttg aaccacttga aacccccgcc agtgggtggg aaataattgg cggtaacggg
     7561 catgggtaaa ccggtcatca attaagacaa tgattccccg gtctgtctcc gtgcggatga
     7621 cccgtccggt tgcctgcagc acccggttga accctgggta ctggtatgca taggcaaacc
     7681 cgctgccgct cttttgatca aaataagcct tgattaaatc acgttctaat cctaactgtg
     7741 gcacccccac accaacaatc acaaccccaa tcagccgttc ccccaccaga tcaatcccct
     7801 cgccgaagat gccgcccatc acagccagcc caaccagggt cgttgtatta ctggctgaaa
     7861 accgcgctaa aaaggcttct cggtcaactt ctgtcattgc tcgttcttga accataagct
     7921 gctcttcagg cagtttatct ttaaggatct caagcacagc tgccagatag gcataggatg
     7981 gaaagaaaac caggtaatta cccacatggg tgttgcacac ggcctcaatg gttgccgcaa
     8041 tctcggcata agaatcagcc cgctgtgcgt atttggtaga tatctgattt tgaattaaca
     8101 ggcaggtatt ctcaatagga aaaggcgatt ggaatatttg tctggggtgg cgtgcgtcac
     8161 cggtcaaaag ccgcataaaa taatccatcg gcagcagcgt ggctgagaaa aagaccgccg
     8221 attgactgtg ctccaatggc gccgccagca ttggcgccgg atccaggcag aacaacttcg
     8281 ccttcaaatc agatgcccct tgtctctcaa agtaagacac ataataggtg tcaaaatact
     8341 cggcagtgcg caggtagttc aggcacagga aatagaatgt cagtaagtcc tgcctgaact
     8401 cggccgcctg attcaaggcc agccagtctt cggccgcctg actgaatttt cgtatggcct
     8461 tgagcaattc ttctggaagc gagttctcta tcagggcttc cttccctgcc gcctggcagg
     8521 ccttgcggat ttgcagcata accttgttga gcgcatttaa tttttgggcc aaatcgggca
     8581 ggtgcggttt tagcgctcgc tgaagctcca gtacgttttt tttatcaatc tcagccgaat
     8641 acatttcccg ggcccggtct ggcaggttgt gcgcctcatc aaccagaaag atgtagggtt
     8701 cggtgcagaa gtcaaaaaag cgatgcagat aaacgcgcgg atcaaacgca tagttgtaat
     8761 cgcagatgat gcaatcggcc cataaagcca ggtcaagcga aaattcgaaa ggacagatct
     8821 ggtactcccg ggcaagtgcc tcaatgactg ggcgggtata agcttgatgc tgatcgatcc
     8881 cttccagtgc ctgcttgacc ttgccaaaat aatctcgcgc aaattcgcaa acctccgggt
     8941 cgcagttcac cggtgggcaa aagcagatct tttctttcgc agtcagggtg acgctgatga
     9001 gctgcaggct tgcctggcgc atatcttcca gcgttttctc tgccacctgc cgcccgggcg
     9061 ttttagcggt caaatagaag atcttggctg ccagcccctg ttccagtgcc ttgactgccg
     9121 gaaaaagggc tgcgatggtt tttcccaccc ccgtagggga ttgcacatac aatcgcccat
     9181 ttgctcggat ggttttgtaa acggccaccg ccatgtctcg ttgacctgag cggtattcag
     9241 cataaggaaa cccaagctgc tgaatggatt gattacgccg ggcttgccag gcgcgcatct
     9301 tgcgcatcca gctcaaataa ggtgtcacta gatcggagaa aaagacttcc aactcagcca
     9361 tcgagaaaat gcgctcaaag cgcttttctt tgcggctgtc cagatggtaa taggtcaggt
     9421 ggacgcaaat tgagtctaaa tggtgctgcc gagcatacat gtaagcatag cactgtgcct
     9481 gtgcccagtg cagccggtta tgttcctcat ttaccaactc cagactcagc gtggttgttt
     9541 tgatctcctc aatgatcacg gggtcctgat gggcatacag gccatcaatc cgcccccgaa
     9601 tttcaagagg cgggtcagcg ccctctacct gataggcaat ttccacctcg gattcatatc
     9661 cttcgggacg tgcccgctgc acctgtttgt gcccccatgt cccttgttga gcacgatcgc
     9721 gtttttgaaa accgcccggc gtaatgtctc ccgcctgcag cgcaaattcc accaacccat
     9781 gaacagagac aataataggt gggcaggatt cagtttgctc gaacgataaa atctccattt
     9841 tcgtcccaga ttctagcaca ggtgccgttt cttcggcagc aattacccct attttgaacc
     9901 ttttattatt gccacgccgg tcatcgcgtt tctgcgtaac aaaacctgtc atttagtcat
     9961 tgttaatcta aaatttaatc cggtatacaa taccttcgcc aaaaatgagt taagcaaata
    10021 gggcttttgt agtaaagttg aggtcgacca aaacaccaac taaccacaaa aagcccatga
    10081 acaatatttt agcactttta caaccgatac ggaacagcgt ttccaaaaca acattgaggc
    10141 agatgagtcg gatcatagcc gcaatgctat caatgacagg gcgtgttacg atgcttgggc
    10201 tgtcacgatg ggcagaaaag ggcggcagct accgaaccgt acagcgcttc tttcacacgg
    10261 cgataccctg ggcaacccta ttctgggggt ttttctgcaa acatctctgg gatccacagg
    10321 agacctacat acttgcagga gatgagagtg tcgtcaccaa agcgggagag aaaacgcacg
    10381 gcctggatta tttcttctca gggttgttga aaagaaccgt tcctgggtta tccttcttca
    10441 ctctgtcgtt gattggcacg cagcggaggc aatcttttcc cgttctggtg aggcaaatgg
    10501 tccgcacgac tgaagaaaag gctgctagcc aggcgaaaaa ggcccaaaaa tctggcgaaa
    10561 ggcgcaaaaa gggtcgtccc aaaggcagtc gtaacaaagg caaagctgaa gtgacgctta
    10621 atccggagtt actccgcatt caagagatgg tgaaaagcct gcttcaaacc atgcaaggcg
    10681 ccattcggtt gtcttacctg gtcatggacg ggcactttgg taatcaccca gcggtgcata
    10741 tggtaagagc atgtggtctc catctgattt caaagctacg ccatgatgct gccttgtata
    10801 tgccctactt a
//
LOCUS       DAOPVS010000059        10720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6228_length_10720_cov_58.996624, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000059 DAOPVS010000000
VERSION     DAOPVS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6228_length_10720_cov_58.996624"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PKL11_06410"
     CDS             complement(<1..1038)
                     /locus_tag="PKL11_06410"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNZ13273.1"
                     /translation="MSTNSTHTSSWSKPVRIVVGLALVIALAVAAWAITQWIYKQTLP
                     FNIPGKVLEYPLWALLVGLAGNLLLKLLNLKETLRPGIRTELFLKIGLVLLGAGINLA
                     VIAKAAGGAIVQTLVIMPTVFFFSWWLGKKFGLDEKLRAVMSTAVSVCGVSAAIAAAG
                     SVSAKKEQVSYITTLVILVALPMMVIAPLLAGAMNLSDPVAGAWFGGNIDTTAAVVGA
                     GTIYSEGAQEIAAIVKSTQNTLIGLVAFLLALWAASHDPKTNNGKPSAKIIWDRFPKF
                     VLGFVLASILFTLGLIDGGKGTAIDALKNWAFTLAFVCMGLEFSIREVRKMGWRPVIV
                     FLIVTVFNSLVG"
     gene            complement(1048..1194)
                     /locus_tag="PKL11_06415"
     CDS             complement(1048..1194)
                     /locus_tag="PKL11_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13274.1"
                     /translation="MSKNRKHLDKPAPKSWLNEDWLAVILAFVIILLAAIGLLGPNGL
                     PIRF"
     gene            complement(1299..1655)
                     /locus_tag="PKL11_06420"
     CDS             complement(1299..1655)
                     /locus_tag="PKL11_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13275.1"
                     /translation="MFFILFVLHDTDALNDVLTAWEECGVSGVTILPSTGLARFRHKG
                     GLREDLPLIPSLDDLTERMENTNRTLFTIVNDQAMVDRVIAATEAVTGNLDLPDTGIL
                     AVLPVAQVKGLNRIQR"
     gene            complement(1655..2890)
                     /locus_tag="PKL11_06425"
     CDS             complement(1655..2890)
                     /locus_tag="PKL11_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNZ13276.1"
                     /translation="MTPFLQLVLVLAIILFAAKTAAYLSTRLGQPAVLGELIVGLLLG
                     PTLIDLTHMVIITDIHLSEIVAELGELGVLALMFLAGLELELRDLTKSGRVSALSGLL
                     GVVAPVGLGLLVGEVSGMDFSHAMFLGLTLGATSVSISAQTLMEMKLLRSRVGLGLLG
                     AAVFDDVLVILLLSGYLALESGGSGLQILLVLGKMLLFLGLSVVVGLWGLPAITRTTS
                     RLPISQGVLTLAIVILLLYGLAAEVVGGMAAITGAFIAGLMFARTPEKAEIEKGLHAL
                     AYSFFVPIFFINIGLNVNLREMNLGVLWLFLGISAVAILGKIIGAGGGARLGGYTWRE
                     SLQMGIGMVSRGEVGLIVAKVGLDQGLLSAEIFSAIVGMVLVTTLVTPPMLRAAFRPP
                     RPARPKPAEPEASEPKELA"
     assembly_gap    2967..3066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3067..4728
                     /locus_tag="PKL11_06430"
     CDS             <3067..4728
                     /locus_tag="PKL11_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNZ13277.1"
                     /translation="KRMKMDLEAESKRWEEQVVQPVIKKNPERKAEFTSPSQIPLPRV
                     ALPQETDYMESLGLPGEYPFTRGVQPTMYRGRFWTMRQYAGFATAEESNRRYRFLLEQ
                     GQTGLSVAFDLPTQIGYDADDPMAMGEVGKVGVSISSRQEMETLFREIPLDKVSTSMT
                     INAPAAVLLAMYIAVARRQGVAEKALRGTIQNDVLKEYVARGTYIFPPKPSLRLITNT
                     FQYCASNVPNWNTISISGYHIREAGSTAVQEVAFTLADAIAYVQAAIESGQEVDKFAS
                     QLSFFFAAHSNFLEEVAKFRAARRLWAQIMRNRFNAKDPKSWMLRFHTQTGGSTLTAQ
                     QPENNIVRVAVQALAAVMGGTQSLHTNSMDEALWLPTEKSVRIALRTQQIIANETGAA
                     DTIDPLAGSYAVEYLTDEISSRAMEYIRKIDEMGGALAAIENGYMQGEIQDAAYRYQR
                     EVESGEQVVVGVNAYQVEEKLELERLTVDPSIEQGARARLAELRATRDAARASELLTH
                     LEAAAKGSENLMPILIECMEGDITLGEACGVLRKVWGEYNPPAWI"
     gene            4755..5168
                     /gene="mce"
                     /locus_tag="PKL11_06435"
     CDS             4755..5168
                     /gene="mce"
                     /locus_tag="PKL11_06435"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNZ13278.1"
                     /translation="MAKITKINHVAIAVPEVDAALTFWRDAMGLAVDHIEDVPSQKAT
                     VVFIPVGESEVELVRPTSDDTGVAKFLAERGGGMHHLCFEVDDIDGMLTQLKEKGVRL
                     INETPLDLPGRRMAFVHPKSTGGVLVELYQLTGAH"
     gene            5184..5630
                     /locus_tag="PKL11_06440"
     CDS             5184..5630
                     /locus_tag="PKL11_06440"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNZ13279.1"
                     /translation="MEQPPLQPHPAQRTLRQLFSVRRLPTLALIALMYVVVALSVVDL
                     TWTLIRDIISPPMILLDIEELLDILNFFLLVLIAIELLDTIIAYLDENVVHVEVVLEV
                     AMIAIARKIIILDIKEVQPLAVIGIGVIIIALAAGFYVVKKTLRHS"
     gene            5787..6044
                     /locus_tag="PKL11_06445"
     CDS             5787..6044
                     /locus_tag="PKL11_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNZ13280.1"
                     /translation="MNNIWQLQDAKSRFSEMVERALAQGVQIVTRRGRKTVVILPYED
                     YERLTAQTDSLAQFLLASPLAGSDLLVERDPRAPRQVDLEP"
     gene            6041..6460
                     /locus_tag="PKL11_06450"
     CDS             6041..6460
                     /locus_tag="PKL11_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNZ13281.1"
                     /translation="MNYLLDTCVLSEFTRRQPDSRVVDWLNAIAEDRLFISVITVGEI
                     QHGIERLPDSHRKTELLVWMNTALLERFAGRMVPIDAPTMHQWGSLTARMEALGQPLG
                     LMDSLIIASALQHNLIVATRNVTDFLPGGVQVINPWE"
     gene            complement(6510..>6693)
                     /locus_tag="PKL11_06455"
     CDS             complement(6510..>6693)
                     /locus_tag="PKL11_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13282.1"
                     /translation="NFSNSPANECAPVWSPDGLHLAWISDMDGNAEIYTAAVTGETLV
                     RLTYTDWANETRLLWH"
     assembly_gap    6694..6793
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6794..7452)
                     /locus_tag="PKL11_06460"
     CDS             complement(<6794..7452)
                     /locus_tag="PKL11_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNZ13283.1"
                     /translation="MLAKRWTLFGILALALVMLALTACSFDPNGLAGTGFEETPTEAV
                     SPDQPTDQPTEAEPTLEPTPTEPPTPTPYPAPVGQIIFIAQANGYNELFGLNASGGEP
                     YPVTAGLMLEQDAVLSPDGSRVAFTANDGENMDIFVVNVDGSGLMRLTDDPARDEQPA
                     WSPDGSWIAFASFRDGNYNVYQVSAWGGEAAPLTDSSDGDMRPIYSPDGAWVIFTSAR
                     DG"
     gene            complement(7627..8535)
                     /locus_tag="PKL11_06465"
     CDS             complement(7627..8535)
                     /locus_tag="PKL11_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13284.1"
                     /translation="MMAPLRRRAAHKKRVWLSLGVILIFAVVAALTPWLAPHNPYRWN
                     LPQSYLPPAWVQDVPTPGSPDFPLGTDYYGRDILSRLIYGTRTGFLLAVTAVSITALI
                     GTLLGLVAGYLGGKADTLINFLIELFQSVPGLMFMVMIALIFRYTLKPNWFNGFITLV
                     IGFSAVGWVSLARLVRVSVQQLKAQLFVEAAVSIGASRWRILLRHILPNVLHVILVWI
                     INAIPAVIMLEVLLGYIGIGLTRATEGDEFNVISWGGLFFDGRKMLTSNPLVVILPSL
                     CILLISMSFILLADFLNESTRPEKTG"
     gene            complement(8532..9572)
                     /locus_tag="PKL11_06470"
     CDS             complement(8532..9572)
                     /locus_tag="PKL11_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13285.1"
                     /translation="MLPFLFKSILRGIAIVLGVTTILFLLMHAIPGNPWMNHSTERRL
                     MLGPALDLDQATMTSLNRRFGLDQPLWRQWLRYVVGDYYGENGEFICGAVCGNLGPSI
                     TQRGRSVQDVLFAPPEGQTFWQSRFGYSIRLMFWSALLAVGLGIPLGIISGTTAKTGL
                     SRAISVILAVIISVPNFILGLLAILVLASWLKIMRVIPNWHNPADWIAPTIVLALTPL
                     AGIARATRAFLINLTGADYIRTAHAKGLHPVRVMRDHLLRNALVPIITYLGPAAMELF
                     TGLFIIEALYAFPGMGREYWLAVLKLDYSMILGLTMLYAVGIVTLNILNTALCELLDP
                     RIREAAVTEAPA"
     gene            complement(9753..>10720)
                     /locus_tag="PKL11_06475"
     CDS             complement(9753..>10720)
                     /locus_tag="PKL11_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13286.1"
                     /translation="RSKNCTMDLDLELEQAIAEEDKPPSTPPRPATAWRVAVITTSRG
                     ETDRLTQRQIAGAETNRQPNLATVFTALRQSGLKPVAIPANADLLSALTDARPDLCLT
                     LNPGAGTPAQAAQLATLLEMLGLPFTGSRPVAQALAADPALRRALWQQAGLPVVNSRI
                     QTGPRFLTLVLGNGANSGAPRSSAFPQRCAADGFCRLPVWRLDNRQMTVTGMAAEQAA
                     AAQMLAIRAHQAIGALDYSLVQLTLGSEGEPRLLSIQTQPDLTPNGAAARLAAAGGWR
                     YAELLSEILTLGGARFGVSVPRPIFRAVRPPLAISARRLAPVSLR"
BASE COUNT         2203 a         3227 c         3183 g         1907 t
ORIGIN      
        1 ccccaccagc gagttgaaga ccgtcacaat caagaagacg atcaccgggc gccagcccat
       61 cttgcgcacc tcgcgaatgg agaactcgag ccccatgcag acaaacgcca gcgtaaaggc
      121 ccagttcttc aatgcatcaa tggcggtgcc tttgccgccg tcaatcaggc cgagggtgaa
      181 gaggatggaa gccagcacaa accccagcac aaacttgggg aagcggtccc agatgatctt
      241 ggcgctgggc ttgccgttgt tcgtcttggg gtcgtgagaa gccgcccaga gcgccagcag
      301 gaaggccacc agcccgatca gcgtgttctg tgtggatttg acaatggcgg caatttcttg
      361 tgcgccctcg ctgtagatgg ttccggcgcc taccaccgcg gcggtggtat caatattgcc
      421 gccgaaccag gccccggcca cggggtctga cagattcatg gcgcctgcca gcagcggggc
      481 aatcaccatc atcggcagcg ccaccagaat caccagggtg gtgatatagc tgacctgctc
      541 tttcttggcc gagacggacc cggccgccgc aatggcagcc gaaacgccgc agaccgatac
      601 tgccgtggac atgaccgcgc gcagcttttc atccaggccg aactttttgc ccagccacca
      661 cgagaagaaa aagaccgttg gcatgatgac cagggtctgc acaatggctc cgccggcggc
      721 cttggcaatc accgccaggt tgatgcccgc ccccagcagc accagcccga tcttaagaaa
      781 cagctcggtg cggatgcccg ggcgcagggt ttccttcagg ttgagcagct tgagcagcag
      841 gttgcccgcc agccccacca gcagcgccca gagcgggtat tccagcactt tgccggggat
      901 gttgaacggc agggtctgct tgtagatcca ttgggtgatg gcccaggcag ccactgccag
      961 cgcaatcacc agcgccagcc caaccacaat ccggacgggt ttggaccagg aagatgtatg
     1021 ggttgaattt gtgctcatgt ctgcctccta aaaccggatg gggaggccgt tgggccccaa
     1081 cagcccgatg gctgccagta aaatgatgac aaaggccaga atgacagcca gccagtcttc
     1141 gttcagccag cttttgggcg ctggcttatc cagatgcttg cggtttttgc tcatcgccat
     1201 cctccgatat tcagtggatt aaatcctggg gatagcttga ttgtacacaa aaacgccgcg
     1261 ctggcggcgc gttttatagg ccgaattttc agcccgcctt aacgctgaat ccggttcaac
     1321 cccttgacct gggcaacagg cagcaccgcc agaatgcccg tatcgggcaa atccaggttg
     1381 ccggtcacgg cttcggtggc agcaatcacc cggtcaacca tcgcctggtc gttgacgatg
     1441 gtgaagaggg tgcggttggt gttttccatg cgctcggtca ggtcgtccag gctggggatc
     1501 agcggcaggt cttcgcgcag cccgcctttg tgacggaagc gcgccagccc ggtgctgggc
     1561 aaaatcgtca cgccgctgac gccgcattcc tcccaggcgg tcagcacatc gttgagcgcg
     1621 tcggtatcgt gcagcacaaa caggatgaag aacattacgc cagctccttt ggttcacttg
     1681 cttccggctc tgccggtttg ggccgcgccg ggcgcggcgg acgaaaagca gcccgcagca
     1741 taggcggcgt gaccagcgtg gtcaccagca ccatgcccac aatcgccgag aagatctcgg
     1801 cgctcaacag tccctggtcc agccccacct tggcgacaat caggcccacc tcgccgcgtg
     1861 agaccatgcc aatgcccatt tgcagcgact cgcgccaggt gtagccgccc agccgcgccc
     1921 ccccgccggc gccaatgatt ttgcccagga tcgccaccgc tgaaatgccc aggaagagcc
     1981 acagcacccc caggttcatc tcgcgcaggt tgacgttgag gccaatgtta atgaagaaga
     2041 ttggcacgaa gaagctgtag gccagcgcat gcagcccctt ttcaatctcg gctttttcgg
     2101 gcgtgcgggc aaacatcaac ccggcaataa acgccccggt gatggctgcc atgccgccca
     2161 ccacctcggc agccaggccg taaagcagca agatcacaat tgccagtgtc agcacgccct
     2221 ggctgatggg cagccgggac gtggtgcggg tgatggcggg caagccccac aaccccacaa
     2281 ccaccgacag ccccaggaag agcagcatct tgcccagcac cagcagaatt tgcaggccgc
     2341 tgccgccgct ttccagcgcc agatagcccg aaagcagcaa aatgaccagc acatcatcaa
     2401 aaacggctgc acccagcagc cccagcccca cccggctgcg cagcagtttc atttccatca
     2461 gcgtttgcgc cgaaatgctc acactggtag cgcccagcgt caggcccagg aacatggcat
     2521 gcgagaaatc catgccactc acttcgccca ccagcagccc caggcccacc ggtgccacca
     2581 cgcccagcag gccgctcagc gccgaaaccc gccccgattt ggtcaggtcg cgcagttcca
     2641 gttccaggcc agccagaaac atcagcgcga gcacgcccag ttcgcccagt tcagccacaa
     2701 tttcgctgag gtggatatcg gtaatgatga ccatgtgggt caggtcaatc agcgtggggc
     2761 cgagcagcag gccaacaatc agttctccca gcaccgccgg ctgccccagc cgcgtgctga
     2821 gataggccgc ggttttggcg gcaaacagaa taatggccag aactaaaacc agttgtagaa
     2881 agggcgtcat gggcaaaacc agccaggtta tgaggattat cgtaagttta ccataagtta
     2941 ttcatccccc gtttgggcgg gcttcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnaaga ggatgaaaat ggatcttgaa gccgagagca aacgatggga agaacaggta
     3121 gtgcagccgg tcatcaagaa gaacccggaa cgcaaggctg agtttacctc gccatcacag
     3181 attccgctgc cgcgcgtggc gctgccgcag gaaaccgatt acatggaatc gctgggcctg
     3241 cccggtgaat atcccttcac ccgcggggtg cagcccacca tgtaccgcgg ccgcttctgg
     3301 acaatgcgcc agtacgccgg gtttgccacc gccgaagaat ccaaccgccg ctaccgcttc
     3361 ctgctggaac agggccagac cggcctttcg gtggcctttg acctgcccac ccagattggc
     3421 tatgatgccg atgatcccat ggcgatgggc gaggtcggca aggtgggcgt ttccatctcc
     3481 tcccgccagg aaatggaaac cctcttccgc gaaatcccgc tggataaagt ctctaccagc
     3541 atgaccatca acgcgcccgc cgccgtgctg ctggcgatgt atatcgccgt ggcgcgccgc
     3601 cagggtgtgg ccgaaaaagc gctgcgcggc accatccaaa atgatgtgct gaaagaatat
     3661 gtggcccgcg gcacctacat cttcccgccc aagccctcgc tgcggctgat caccaacacc
     3721 ttccagtact gcgcgagcaa tgtgcccaac tggaatacca tcagcatctc cggctaccac
     3781 atccgcgagg cgggttcaac cgccgtgcag gaagtggcct ttaccctggc cgatgccatc
     3841 gcctatgtgc aggcggcgat tgaatcgggt caggaagtgg acaagtttgc ctcgcagctt
     3901 tcgttcttct ttgcggcgca cagcaacttc ctggaagaag tggctaaatt ccgcgctgcc
     3961 cgccgcctgt gggcgcagat tatgcgcaac cgcttcaacg ccaaggaccc caagtcctgg
     4021 atgctgcgct tccacaccca gaccggcggc tccaccctca cggcccagca gcccgagaac
     4081 aacatcgtgc gtgtggcggt gcaggccctg gctgcggtga tgggcggcac acagtcgctg
     4141 cacaccaaca gcatggatga ggcgctctgg ctgcccaccg aaaaatctgt ccgcattgcc
     4201 ctgcgcaccc agcagatcat cgccaacgaa accggcgccg ccgacaccat tgacccgctg
     4261 gcgggctcgt atgcagtgga atatctgacc gatgagatta gcagccgcgc catggaatac
     4321 atccgcaaga ttgacgagat gggcggcgcc ctggctgcga ttgaaaacgg ctatatgcag
     4381 ggcgaaattc aggatgcggc ctaccgctat cagcgcgagg ttgaatcggg cgagcaggtt
     4441 gtggtcggcg tgaacgccta ccaggtggaa gaaaaactgg aactggaacg cctgaccgtt
     4501 gatccttcca ttgaacaggg cgcccgcgcc cgcctggcag agctgcgcgc cacccgcgat
     4561 gccgcccgcg ccagcgaact gctgacccac ctggaagctg ccgccaaagg cagcgagaac
     4621 ctgatgccca tcctgatcga atgcatggaa ggcgacatca ccctcggcga ggcctgcggc
     4681 gtgctgcgca aggtttgggg cgaatacaat ccccccgcct ggatttagtc tggacttaaa
     4741 ttaggagtaa cccaatggct aaaattacca aaatcaatca tgtggcaatt gccgtccccg
     4801 aagtagatgc tgccctgacc ttctggcgcg atgccatggg cctggcggtg gatcatatcg
     4861 aagatgtgcc cagccagaaa gccaccgtgg ttttcatccc cgtgggcgaa agcgaagtgg
     4921 aactggtgcg ccccaccagc gatgacaccg gcgtggccaa attcctggcg gagcgcggcg
     4981 gcggcatgca ccacctgtgc tttgaggtgg acgatattga cggtatgctg acccagctca
     5041 aagaaaaggg cgtgcgcttg atcaacgaaa ccccgctcga cctgccgggc cgccgcatgg
     5101 cctttgtgca ccccaagagc accggcggcg tgctggtaga actgtatcaa ctgaccggcg
     5161 cccactaatt tctggcggcc tccatggaac aacccccgct ccaacctcac ccggcccaac
     5221 gcaccctccg acagttgttc tcggtgcggc ggctgcccac cctggcgctc atcgccttga
     5281 tgtatgtggt ggtggcgctg tcggtggtgg acctgacctg gacgctgatc cgggatatca
     5341 tctcgccgcc catgattttg ctggatattg aggagctgct ggatatcctc aacttcttcc
     5401 tgctggtgct gattgccatc gagcttctcg ataccatcat cgcctacctg gatgaaaacg
     5461 tggtgcacgt ggaggtggtg ctggaagtgg cgatgattgc catcgcgcgc aaaatcatca
     5521 ttctggacat taaagaagtg cagccgctgg cggttattgg gatcggggtg attatcattg
     5581 ccctggcggc gggtttctac gtcgtcaaaa agacgctgcg gcactcttga ggcctggctt
     5641 ttcacaaaaa atcaaaccgg aggcgggtga ctgcctccgg ttttgctgtg cctggcctgc
     5701 ctgcagccta aggggcttga ttcttttcca ttctgccggt ataatcttat cagactagtc
     5761 tgactagtct gataaggagg tgcagtatga acaatatctg gcagcttcaa gatgccaaga
     5821 gccgtttcag tgaaatggtg gagcgcgccc tggcccaggg cgtgcagatt gtcacccggc
     5881 gcggccgaaa aacggtggtc attttgccct atgaagatta cgagcgtctg accgcccaga
     5941 ccgacagcct ggcgcagttc ctgctggctt cgcccctggc cgggtctgac ctgttggttg
     6001 agcgcgaccc ccgggcgccg cgccaggttg accttgagcc atgaactatc tgctggacac
     6061 ctgcgtgctt tccgaattta cccgccgcca gccagacagc cgcgtggtgg actggctgaa
     6121 tgccattgcc gaagaccggc ttttcatcag cgtcatcacc gtgggcgaaa ttcagcacgg
     6181 catcgagcgc ctgcccgact cgcaccgcaa gacagaactg ctggtgtgga tgaacactgc
     6241 cctgctggag cgctttgccg ggcgcatggt gcccatcgac gcgcccacca tgcaccaatg
     6301 ggggtctctc acggcccgaa tggaggccct gggccagccg ctggggttga tggattcgct
     6361 catcattgcc agcgcactgc agcataatct gattgttgct acccgcaatg tgaccgattt
     6421 tctccccggc ggggttcagg tcatcaatcc ctgggaataa gcaccgccag caggttcacg
     6481 ctgctggcgg ctgttttttt gtgcggcccc taatgccaga gcagccgggt ttcgttggcc
     6541 cagtcggtat aggtcaggcg gaccagggtt tcgccggtga cggcggcggt gtaaatttcg
     6601 gcgttgccgt ccatgtctga aatccaggcc aggtgcaggc cgtctggcga ccagacgggc
     6661 gcgcactcgt tggccggcga gttgctgaag ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnccatcgc gcgcgctggt gaagatcacc caggcgccat cgggggagta
     6841 gatcgggcgc atatcgccat cgctgctgtc ggtcagcgga gcggcctcgc cgccccaggc
     6901 gctgacctga taaacattgt aattgccatc ccggaaggag gcaaaggcaa tccaactgcc
     6961 atccggcgac caggcgggtt gttcgtcacg ggcggggtca tctgtcaggc gcatcagacc
     7021 gctgccatcc acattgacca caaaaatatc catgttttcg ccatcgttgg cggtgaaggc
     7081 cacccggctg ccatcgggcg aaagtacggc gtcctgctcc agcatcaacc cggcggtcac
     7141 cgggtagggc tcgccgccgc tggcgttcag cccgaacagc tcgttatagc cattggcctg
     7201 ggcaatgaag atgatctgcc ccacaggggc ggggtaaggc gtgggggtgg gcggctcggt
     7261 gggggtgggc tccagggtcg gctcggcctc agtgggctgg tcggtgggct ggtctggcga
     7321 tacagcttcg gtgggggttt cttcaaagcc ggtgccggcc aggccgttgg ggtcaaatga
     7381 acaggctgtc agcgccagca tcaccaacgc cagagccaga attccaaaga gggtccatct
     7441 ttttgctaac attaatatta tgcctttcaa ctagtttgat tgtattagtc gaagcgcaga
     7501 taggaaaggg gtcaggcctt gcaaagttgt aaggactcgg acaatttgga gtacaaaatg
     7561 cagttttgag attgcagaat tgaaatctgc cccgcaagtg cagcgcgaaa cgacgcgcgg
     7621 gatgcctcag ccggtcttct cggggcgggt cgattcgttc aaaaaatcgg ccagcaggat
     7681 gaaactcatt gaaatcaaca ggatgcagag cgatggcagg atcaccacca gcgggttgct
     7741 ggtcagcatc ttgcggccat caaaaaacag gccgccccag ctaatcacgt taaactcgtc
     7801 gccttcggtg gcgcgcgtca ggccaatgcc aatatagccg agcagcactt ccagcataat
     7861 gacggccggg atggcgttga tgatccagac caggatcacg tgcagcacat tgggcagaat
     7921 atgccgtaag aggatgcgcc accgggaggc cccaatgctg accgccgcct cgacaaacaa
     7981 ctgtgccttg agctgctgca cactcacacg caccagccgc gccagactga cccagcccac
     8041 cgccgaaaaa ccaatcacca gggtgatgaa tccattgaac cagttgggct tcagggtgta
     8101 gcggaaaatc agggcgatca tcaccataaa catcagcccc ggcaccgact gaaacagctc
     8161 aatcaggaag ttaatcaggg tgtcggcctt gccgcccaga taccccgcca ccaggcccag
     8221 cagcgtgccg atcagcgcgg tgatggaaac agccgtgacc gccagcagaa agcccgtgcg
     8281 cgtgccataa atcaaccggc tgaggatgtc gcggccgtaa taatcggtac ccagcggaaa
     8341 atcggggctg ccgggcgttg gcacatcctg cacccaggcc gggggcaaat aactctgcgg
     8401 cagattccag cggtaagggt tgtgcggcgc cagccagggc gtcagcgcgg ccaccaccgc
     8461 aaagatcaga attaccccca ggctgagcca gacccgtttt ttgtgcgctg cccgccgccg
     8521 gaggggggcc atcatgccgg cgcctcagtg actgcggcct cacggatccg gggatccagc
     8581 agctcgcaca gcgccgtgtt tagaatattc agcgtcacaa tcccaacggc gtacagcatg
     8641 gtcagcccca ggatcatcga ataatccagc ttcaacacgg caagccaata ttcccgcccc
     8701 atgccgggga aggcatagag ggcttcgatg ataaacaacc cggtgaacaa ttccatggcg
     8761 gcgggcccca ggtaggtgat gatgggcacc agggcattgc gcagcaggtg gtcgcgcatc
     8821 acccggacgg ggtgcagccc tttggcatgt gccgtgcgga tataatctgc gccggtgagg
     8881 ttaatcagaa aggcccgggt tgcgcgggca atcccggcca ggggcgtcaa ggccaggacg
     8941 atggtcggtg caatccaatc ggccgggtta tgccagttgg gaatgacccg cataatcttc
     9001 agccaggagg ccagaaccag aatggccagc agccccagga tgaaattggg gaccgagatg
     9061 atgaccgcca gaatcacaga aatggcccgg ctcaggccgg ttttggcggt ggtgccgctg
     9121 ataattccca gcggaatccc cagtccaacg gcgagcagcg cgctccagaa catcaaccgg
     9181 atggagtagc caaaacggct ttgccagaag gtttggcctt cgggcggggc aaaaagcaca
     9241 tcttgcacac tgcgcccgcg ctgcgtgatg gagggaccca ggttgccgca aaccgcgccg
     9301 cagataaatt caccattctc gccataataa tccccaacga catagcgcag ccactgccgc
     9361 cagaggggtt ggtccagccc aaagcgccgg ttgaggctcg tcatggtggc ctggtccaga
     9421 tccaaggcgg ggcccagcat caggcggcgc tcggtagaat gattcatcca cgggttgccc
     9481 gggatggcat gcatcaacag aaatagtatc gttgtcaccc ccaggacaat ggcaatcccc
     9541 cgcaaaatgg acttaaacaa aaaaggcagc atggttgaga ttttctgggg cgaagagccg
     9601 ctccatgtac tttgattata cctgttttga ccccgcgggc gcagatcctg taaacagcca
     9661 accattcatc cggatgctgc gcggctaaaa aattcccccg acggtaagac gcgccggggg
     9721 gtgttggttt acgggttacc gcctgtgcgg tgtcagcgca gcgagacggg cgccagacgg
     9781 cgggcgctga tggccagcgg cgggcgcacc gcccggaaga tgggccgcgg caccgagacc
     9841 ccaaaacggg ctccgcccag ggtcaggatt tcggacagca gctcggcata gcgccaaccg
     9901 ccagccgcag ccagacgggc cgcggcgccg ttgggcgtga ggtcgggctg ggtctgaatg
     9961 gacagcagcc ggggttcgcc ctcgctgccg agggtgagct gcaccagcga atagtccagt
    10021 gcgccaatgg cctggtgggc gcggatcgcc agcatttgtg cagcggcagc ctgttcggcc
    10081 gccatgccgg ttaccgtcat ctggcggtta tccagccgcc agaccggcag acggcagaag
    10141 ccatcggcgg cacagcgctg cgggaaggcg ctgctgcgcg gcgcgccgga gttggctccg
    10201 ttgcccagca ccagcgtcag aaagcgcggg ccggtctgga tgcggctgtt gaccaccggc
    10261 aggccagcct gctgccacag ggcgcggcgc agggcagggt cggcagccag cgcctgcgcc
    10321 accggccgcg agccggtgaa gggcaggccc agcatttcca gcagggtggc taactgcgca
    10381 gcctgggccg gggtgccagc gccgggattc agcgtcaggc agaggtcggg gcgggcatcg
    10441 gtcagcgcgc tcaatagatc cgcattggct ggaatggcaa cgggtttcag cccgctctgg
    10501 cgcagtgcgg tgaaaacggt cgcgagattg ggttggcggt tggtttccgc tccggcaatc
    10561 tggcgttggg tcagccgatc agtctctccc cgtgatgttg tgatcacggc tacccgccat
    10621 gcggttgccg gccgcggcgg agtactggga ggcttgtctt cttcggcgat ggcttgttcg
    10681 agctcgagat caagatccat ggtacagttt ttactccgat
//
LOCUS       DAOPVS010000060        10623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6303_length_10623_cov_48.052139, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000060 DAOPVS010000000
VERSION     DAOPVS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6303_length_10623_cov_48.052139"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..782
                     /locus_tag="PKL11_06480"
     CDS             18..782
                     /locus_tag="PKL11_06480"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HNZ13287.1"
                     /translation="MTALAFTEAVQTAAQYLREARSVVALTGAGLSTPSGIPDFRSAG
                     SGLWTQNDPMKVSSLTAFRQRPERFFNWLRPLAQQILAAAPNAAHAAMAELEQAGRLK
                     AVITQNIDGLHQRAGSQNVIELHGSLRQAHCVHCHQAYSLELYAPAFIDREQIPRCPD
                     CGWVLKPDIILFEEALPIKALEEAQALCESADLILIAGTALEVSPANRLPMFAMENGA
                     RMIINTLSATPLDMFADMLMPYDVAQALPAIAKEVL"
     gene            779..1972
                     /locus_tag="PKL11_06485"
     CDS             779..1972
                     /locus_tag="PKL11_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ13288.1"
                     /translation="MTNPNLPLERYQRLIEISRDLASTLNLDDLLQRIIIMAMELVEA
                     EASSILLYDERKNQLTFSVANDPRDQAVLAGIIVPPESIAGWVATNREPVFVPDVHAD
                     ARFFKDVEQALQFTTRSIIAVPMMARDRLIGVLEVLNKKRDVFNEADLALLQALSAQA
                     AVAIQNSRLFHQADLISELVHEIRTPLSAISTVAYLLQRPGMTDVQRLEMAGMIRRET
                     ERLNELASSFLDLARLESGRMAIHPAPINLAGLIDECCKVTRPKAEENGIIIRVQLPH
                     QIPSIEVDPEKFKQVLLNLLSNAVKYNQPNGKVTVTVTADEDDLQIAVADTGAGIPAE
                     ALPHIGEKFFRAKNTEGSHPGTGLGLSICKWIVEAHGGRLTIESTVNVGTTFTVHLPI
                     SKAEG"
     gene            complement(1969..2838)
                     /locus_tag="PKL11_06490"
     CDS             complement(1969..2838)
                     /locus_tag="PKL11_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ13289.1"
                     /translation="MQIVTDRGADFSAEQLKDLNIHFAPLRIQLEGKTYVSGIDITSE
                     EFYDRISKTESYPTTSQPSAGEFAELYRELAKTGPDILSVHISSGLSGTVNAARLGAE
                     MVPEAKVTIWDTKILSCPEAWQVEAAARAVKAGWDLPRILTMLETLRKRITGIYTLND
                     LKYLIHGGRINHITGLVASLLNIKPIITVDKESGVYIQAGREITLKRAIGRMAHIVEN
                     WYGSTGEALRIQLLHGLNLEGVEMFREELTKRLSNIHWLPTANIAPVLGAHTGPSMVG
                     CAVGPLNLFEALP"
     gene            complement(2863..4650)
                     /gene="mutL"
                     /locus_tag="PKL11_06495"
     CDS             complement(2863..4650)
                     /gene="mutL"
                     /locus_tag="PKL11_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNZ13290.1"
                     /translation="MPIQILPDEVASQIAAGEVVERPASVVKELVENALDAGASHIVV
                     RLAEAGRRLVEVADDGAGILADEMQLAVARHATSKLRTAEDLFHITTLGFRGEALASI
                     ASVSRFSLTSRRQDVSVGARLTVAGGRIGALESVGVPVGTVVRVEDLFYNVPARLKFL
                     KRDQTERQQVDALLTRYALAYPAVRFELFHDDKPALQTSGNGDSREVLAQLLGVDVAR
                     QMLAVSLEDEGLTINGYISPVALTRSNRREITFFINGRWVQDTALTAALLHSYHTLLM
                     VGRYPIAVLFVELTPEDVDVNVHPAKAEVRFRTPDRVFGAVQRATRRALLAYSPVPPV
                     STSYWRSAPSSGPAIDPAWDMSADLRGLPAQPELTVAPSLDEPSTHISPDAILPTPPA
                     SNSPSHLPSGQIPLLRLIGQVGGAYLVAEGPDGLYLIDQHAAHERVLFERLMQSHGSL
                     PAQALLTPVVLQLPAPQAQLLQNQLDTLSHLGFQVELFGPNTFRILAIPTIFSGGDPL
                     AALRVVVEDFEEDETPLQTEVEDRIAARVCKRMAVKAGQILSVDEQRALVRDLETCQS
                     PRTCPHGRPTMIHLSVDLLERQFGRRGSR"
     gene            4772..5410
                     /locus_tag="PKL11_06500"
     CDS             4772..5410
                     /locus_tag="PKL11_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNZ13291.1"
                     /translation="MRFNTLFFDLDETVYPSSSGVWAAIRDRMQRYMIEQLGIPAEEV
                     PALRERLYREYGTTMRGLQYERGLDTDAFLAYVHDIPLVNYLSPAPSDREALLSLPQQ
                     RFIFTNADVHHARRVLNILNLSDCFEGIMDITMFIPHCKPQPEAFEIALKKAGSPDPT
                     RCVLIDDLPVNLKAARQMGFSTILISENPPENHDFDAHIRSLSELPTVLTAL"
     gene            5474..6703
                     /locus_tag="PKL11_06505"
     CDS             5474..6703
                     /locus_tag="PKL11_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ13292.1"
                     /translation="MSKAIRVILVIVVSLGVLACAFAGGVAVGWGIPRSITSKVPVPM
                     PSDTGAAETGTSDDLADLFAPFWEAWDIVHAQYVDQPVDDLLLMQGAIRGMMDSLGDP
                     HTSYMDPVEYETANAPLEGSYEGIGAWVDTSGEWLTIISPIEGSPAEAAGLKPDDRIV
                     GVDGEDMTGVDPELVRRKVLGPAGSPVVLTIAREGEAETFEVTLTRATVVLKSVNYEM
                     LDGQIAYVEVTTFGDDTTSELKKALTDLMAQNPKGLILDLRNNGGGYLITAIEVVSQF
                     IGDNQVIMYEQFGDGSRETYNALPGGLALDVPMVVLVNEGSASASEITAGALQDYGRA
                     QLVGVTTYGKGSVQTWNELDNNQGAVRVTIARWLTPKERLIHEVGLTPDVVVEITDED
                     VDAGRDPQLEKAIELLK"
     gene            6795..7661
                     /locus_tag="PKL11_06510"
     CDS             6795..7661
                     /locus_tag="PKL11_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNZ13293.1"
                     /translation="MEINPAADTNRATEIKSKMHFTGTVKTITLAGAVIDIGVGTPAV
                     LHISQMAASTEGQPIKSVREVLQEGQTIDVWVRRVKDNRVELTMFRPLDLEWRDLKKG
                     MVVKGKVVRLEKFGAFIEIGAERPGLVHISEMAHSYVRLPSDVVNEGDEVEAEVIDVD
                     RKKKQIKLSMKALQPEPVKEEEPEMPVEMAPRRERTERGERGERPARRRTTSKGGRRE
                     HQEDNSEAMAALAAEAAAAEAEPTAMGLAWQEAMEKAKTRREERARKGKSGATSEHEE
                     IFSRTLEQKSQQ"
     gene            7799..>8394
                     /locus_tag="PKL11_06515"
     CDS             7799..>8394
                     /locus_tag="PKL11_06515"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNZ13294.1"
                     /translation="MPTKYIFFTGGVVSSVGKGVTAAAVGRLLKERGFRVAVQKLDPY
                     INVDPGTMSPYQHGEVFVLDDGAETDLDLGHYERLIDIRLNRVCNITTGQVYAEVISK
                     ERRGDYLGGTIQVIPHITNEIKRRIGLVAKTTQAEIVLVEVGGTVGDIESLPFLEALR
                     QLRSDLGRENTFYIHVTWLPHIGATNELKTKPTQHSVR"
     assembly_gap    8395..8494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8495..9253
                     /locus_tag="PKL11_06520"
     CDS             <8495..9253
                     /locus_tag="PKL11_06520"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNZ13295.1"
                     /translation="PKPTVKVALVGKYVELHDAYISVREAIMHAALTAGVEVDLRWVH
                     SADLERGQGLEQLEDVDGIVVPGGFGSRGIEGKIQAAQQARTRKIPYLGLCLGMQLMV
                     VEFARYALGNEKANSTEFDQSTPCPVIDLMPDQQNITDMGGTMRLGLYPCNLQPDTIA
                     ARAYSEPQVQERHRHRFEFNNNYRQVLYDKGLRFSGLSPDGKLVEIAELVEHPFMVGT
                     QFHPEFLSRPNRPHPLFAAFMEAVRDHAGVTEKS"
     gene            9320..10612
                     /gene="eno"
                     /locus_tag="PKL11_06525"
     CDS             9320..10612
                     /gene="eno"
                     /locus_tag="PKL11_06525"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ13296.1"
                     /translation="MDTIIESVIGQEVLDSRGNPTVEVEVILANGAWGRAAVPSGAST
                     GVHEALELRDGDKKRYGGKGTLQAVQHVNEDIAELIVGWDAVEQKAIDMAMIEADGTP
                     NKSKFGANAILGTSLAVAKAAAEALGLPLYRYIGGVYAHVLPVPMLNILNGGAHTGWQ
                     STDAQEFMVMPLGAPSFAEGLRWGAEIYHALKAVLKARGYATLVGDEGGYAPALKANV
                     EAVEVILEAITKAGYKPGEEVCIALDPAASELFDEDSKKYNLRREGKLLTGEQMVAFW
                     AEWVRQFPIVSIEDGLAQDDWDSWKLMVRELGSKLQIVGDDLLVTNPERVRRAIKEQA
                     ANALLVKLNQIGSLTETIEAVETCHRAGWRAVTSHRSGETEDSTIADLAVALNMGQIK
                     TGAPARSDRVAKYNQLLRIEAELGETATYAGWSALRRG"
BASE COUNT         2394 a         2655 c         3295 g         2179 t
ORIGIN      
        1 gttgaccgcc tgaacaaatg accgcgctgg cttttacaga agcagttcaa acggcagccc
       61 agtatctgcg ggaagcccga tctgttgtcg cacttacggg tgccggactt tccacgccat
      121 cggggattcc ggattttcgc tcggcgggat cggggttgtg gacgcaaaac gatccgatga
      181 aggtttcttc attaactgca ttccgccagc gcccggagcg ctttttcaac tggctgcgcc
      241 cgctggcaca gcaaattctg gctgcggcgc caaatgccgc ccatgcagct atggcggagt
      301 tggaacaggc agggcggctg aaagcggtga tcacccaaaa tatcgatgga ctgcatcaac
      361 gggcgggcag tcagaacgtg atcgagctgc acggcagcct gcggcaggcg cactgtgtgc
      421 attgccatca ggcttactcg ctggagcttt atgccccggc atttattgac cgggagcaaa
      481 ttccccgctg cccggactgc gggtgggtgc tcaaaccgga tattatcctg tttgaagaag
      541 cactgccgat taaagcgctg gaagaagccc aggcgctgtg tgaaagcgca gatttgatac
      601 taattgcggg gacggctctg gaagtatctc cggcaaaccg actgcccatg tttgccatgg
      661 agaacggcgc gcggatgatc atcaatacac tctctgccac gccactggat atgtttgcgg
      721 acatgttaat gccttatgat gtggcccagg ccctgccagc aattgcgaag gaagtcttat
      781 gaccaacccc aatttaccat tggagcgtta tcagcgtttg attgaaatct cgcgcgatct
      841 ggcctccacg ctcaatctgg atgacctgct gcaacgcatc atcatcatgg caatggaact
      901 ggtggaagca gaagccagtt ctattttgtt atatgatgag cgcaaaaatc aacttacttt
      961 ttcagttgcc aatgatccgc gcgatcaggc tgtgctggcg ggcattattg tgccgccgga
     1021 aagcattgcg ggctgggtgg cgacaaaccg ggaaccggtg tttgtgcctg atgtgcatgc
     1081 ggatgcccgt tttttcaagg atgttgagca ggcgctgcag ttcactaccc gttcgatcat
     1141 tgcggtgccg atgatggcgc gcgaccggct gatcggggta ttggaggtgc tgaacaaaaa
     1201 gcgcgatgtg tttaatgaag ctgacctggc tctgctgcaa gccctgagcg cccaggcagc
     1261 ggtggcgatt caaaattcgc ggttatttca ccaggcagac ctgatttctg aactggtgca
     1321 tgagattcgc accccgctgt cggcgatcag cacggtggct tacctgctgc aaaggccggg
     1381 gatgacggat gttcaacggc tggaaatggc agggatgatc cggcgggaaa cggaaaggct
     1441 gaacgaactg gcctcctcat ttttggattt ggcgcggttg gagtcggggc ggatggcaat
     1501 tcacccggcg ccgattaacc tggcgggatt gattgatgaa tgctgcaagg tgacacggcc
     1561 caaagcggaa gaaaatggga ttattattcg ggtacagctc ccccatcaga taccctccat
     1621 tgaggtggat ccggagaaat tcaagcaggt gctgctcaac ctgttgagca atgcggtgaa
     1681 atataatcaa ccgaacggga aggtgacggt gacggtaacg gctgatgaag atgatttgca
     1741 gattgcagtt gcggatacgg gggcgggcat tccggcagag gcgctgccgc atattggcga
     1801 gaaattcttc cgggccaaaa atacggaggg cagtcaccca gggacagggt tggggctttc
     1861 gatttgcaaa tggattgttg aggcacacgg cgggcggctg acaattgaaa gcacggtgaa
     1921 cgtggggacc acattcaccg tgcatttacc gatttcaaag gctgagggtt agggcaaagc
     1981 ctcgaataaa ttcagggggc cgacggcaca gccaaccata ctggggccgg tgtgggcgcc
     2041 cagcaccggg gcaatattgg ccgtgggcag ccaatggatg ttggacagac gtttggtcag
     2101 ttcctcacgg aacatctcca caccttccag gttgagtccg tggaggagct gaatacgcaa
     2161 ggcttcgccc gtactgccgt accagttttc cacaatgtgg gccattcggc caatggcgcg
     2221 cttaagggtg atttcacgcc cagcctgaat gtagacaccc gattctttat ccacagtgat
     2281 gatgggtttg atattcagca gggaggctac cagccccgtg atgtggttga tgcgcccgcc
     2341 atggatgagg tatttgaggt cattcaaggt atagataccg gtaattcttt tacgcagggt
     2401 ttccagcatg gtgaggatac gggggagatc ccagcccgct ttgaccgccc gggcagcggc
     2461 ttccacctgc caggcttcgg ggcaggacag gattttggta tcccaaatgg tgaccttagc
     2521 ctcgggcacc atctcggcgc caaggcgggc ggcgttcacg gtgccgctga ggcctgaaga
     2581 aatatgaacc gacaggatat cggggccggt tttggcaagt tcgcgatata gttcggcaaa
     2641 ctcaccggcg gatggttggg aggtggtagg ataggattcg gttttggaaa ttcggtcgta
     2701 aaattcctcc gaagtaatat caatcccact gacgtaggtt ttgccctcca attgaatacg
     2761 caagggggca aaatggatat tgaggtcttt caattgttct gccgaaaagt cggccccacg
     2821 gtcggtcacg atttgcatct tcttctcctg tttgaaaata ggttagcggc tgccacgccg
     2881 cccaaattgg cgttccaata agtccacgga aagatgaatc atggtggggc gaccatgcgg
     2941 gcaggtgcgg ggagactggc aagtttccag gtctcgcacc agggcgcgtt gttcgtcaac
     3001 ggataaaatt tgcccggctt tgaccgccat gcgtttgcaa acccgggcag ctatacgatc
     3061 ttccacttcc gtttgaagtg gggtttcatc ttcttcaaaa tcttctacca ccacccgcag
     3121 ggcggctaga ggatcaccgc cagaaaaaat tgttgggatg gctaaaattc gaaaagtatt
     3181 agggccaaat aattccacct ggaagccgag gtgcgagagg gtatcgagtt gattttgaag
     3241 cagctgggcc tgtggggccg gaagttgtag tactaccggg gtgagcaatg cctgggcggg
     3301 gaggctgccg tgagactgca tcagacgctc aaacaggacg cgctcgtgag ctgcatgctg
     3361 atcgatcaga taaaggccat cggggccctc ggcaaccaga taggcgccgc caacctggcc
     3421 gatgaggcgc aggaggggga tttgcccgga ggggagatgg gatggtgaat ttgaggccgg
     3481 aggcgtgggc aggatagcat ctggcgagat gtgggttgag ggttcatcca atgagggggc
     3541 aacagtcagt tcgggctggg caggaagacc gcgcaggtcg gcagacatat cccaggcggg
     3601 gtcaattgcg gggccggagg acggcgctga acgccagtaa gaggtcgaga cgggcgggac
     3661 agggctgtag gccaacagcg cccggcgggt ggctcgctgc accgcaccaa agacacggtc
     3721 gggggtgcgg aaacggacct cagctttggc tggatgaaca ttgacatcca catcttcggg
     3781 ggtgagttca acaaataaga cggcgatggg gtaacggccc accatgagca gtgtatgata
     3841 agaatggagc agagctgcgg tcagggcagt gtcttgcacc cagcggccat tgataaagaa
     3901 ggtgatttcg cggcggtttg agcgggtgag ggccacaggg ctgatatagc cattgatggt
     3961 caaaccctca tcttcaagac tgacagccag catctggcgg gcaacatcca cgccgaggag
     4021 ctgggccagg acttcacgtg agtcgccgtt gccgctggtc tggagggcgg gtttgtcgtc
     4081 gtggaagagt tcaaagcgca cggcggggta tgccagggca tagcgcgtga gcagtgcatc
     4141 aacctgctgg cgctcggttt gatcgcgttt aagaaatttg agccgcgccg gaacgttgta
     4201 aaagaggtct tcaacccgca caaccgtgcc aaccggcaca ccaaccgatt cgagggcacc
     4261 aatccgcccc ccggcaaccg tcagacgggc gccaaccgaa acgtcctggc ggcgtgaggt
     4321 gaggctgaaa cgggaaaccg aggcgatgga ggccagggcc tcgccccgaa agccgagggt
     4381 tgtgatgtga aagagatctt cagcagtgcg cagtttgctg gtggcgtggc gggccactgc
     4441 caactgcatt tcatcagcca aaatacccgc accatcatcg gcaacttcaa ccagacgccg
     4501 accggcttcg gccaggcgca ccacaatatg gctggcgcca gcatccaggg cgttttcgac
     4561 aagctctttg acgaccgagg cgggacgttc aaccacctcg ccggcggcga tttgagaagc
     4621 aacttcatcg ggcaggattt ggataggcat tgtcaattcc aaattttaat tatatcagaa
     4681 tgataccgct cggtatggta aatcacgaaa ctcagtttat gaaaaagata agaagctatt
     4741 ctcgggggcg gcgtgtgcta taatacgccc catgcggttc aacactttat ttttcgacct
     4801 cgatgagacg gtttatccgt catcaagcgg ggtttgggcg gcaattcgag atcgaatgca
     4861 gcgctatatg attgagcaac tgggcatccc ggcagaggaa gtgccggcgt tgagggaacg
     4921 gttgtatcga gaatatggga caacgatgcg cggactgcaa tatgagcgtg ggctggatac
     4981 cgatgctttt ctggcctatg tgcacgatat tccgctggta aattacctgt cccctgcccc
     5041 gtcggatcgc gaggcgctgc tctcgctgcc gcagcagcgg tttatcttta ccaatgccga
     5101 tgttcaccat gcccggcggg tgctgaacat actcaacttg agcgactgct ttgaggggat
     5161 tatggacatc acgatgttta taccgcactg caaaccgcaa cccgaagcat ttgaaattgc
     5221 gctgaaaaaa gccggatcac cggacccgac ccggtgtgtg ttgattgatg atctgccggt
     5281 gaacttaaaa gccgcacgac agatggggtt ttccacgatt ttaatcagcg aaaatccacc
     5341 tgaaaatcat gattttgatg ctcatattcg ttcgctgtca gaactaccta cggttctgac
     5401 ggcgttatga cggcggcgcg ccaaagctcg gcagccgtca gtttatttag attaagaaag
     5461 gatactggtg aaaatgtcta aagccattcg cgttattctc gtcattgtcg tttcactcgg
     5521 tgtgctggcc tgcgcgtttg caggcggcgt ggctgttggc tggggcatcc cccgcagcat
     5581 tacgtccaag gtacccgttc ccatgccatc ggatacgggg gcagccgaaa ccggcacctc
     5641 ggatgattta gcggatctat ttgcgccctt ctgggaagcc tgggatattg tgcatgccca
     5701 gtatgttgac cagccggtgg acgatctgct gctcatgcag ggtgccatcc gtgggatgat
     5761 ggattcgctg ggtgatccgc atacgtctta catggacccg gtggaatacg aaactgccaa
     5821 cgcccccctg gaagggtcgt acgaagggat tggggcctgg gttgatacca gcggcgagtg
     5881 gctgacgatc atcagcccga ttgagggctc gcctgccgaa gctgccggac tgaaaccaga
     5941 tgaccggatt gtgggcgtgg atggcgaaga tatgaccggg gttgacccgg aactggtacg
     6001 ccgcaaggtg ctgggaccgg cggggtcgcc agtggtgctg accattgccc gcgaaggtga
     6061 agccgagacc tttgaggtca ccctgacgcg cgcaacggtg gttttgaaga gtgtgaatta
     6121 tgaaatgctg gacggacaga ttgcctacgt tgaagttacc acctttggcg atgacacaac
     6181 cagtgagtta aagaaggctc tgaccgacct gatggcgcag aatcccaaag gtttgattct
     6241 ggatttgcgc aacaacggcg gcggctacct gatcaccgct atcgaggtgg tgtcgcagtt
     6301 tattggcgac aaccaggtga ttatgtatga gcagtttggg gatggctcgc gtgaaaccta
     6361 taatgccctg cccggcgggt tagccctgga tgtgccgatg gtggtgctgg tcaacgaagg
     6421 gtcagcctca gcttccgaga ttacagccgg tgcgctgcag gattatgggc gggcgcagct
     6481 ggtgggcgtg accacctacg gcaaaggctc ggtgcagacc tggaacgagc tggataataa
     6541 tcagggtgcg gtgcgcgtga cgattgcgcg ctggctgacg cccaaggaac gcctgatcca
     6601 cgaggtaggt ttgacgccgg atgtggtcgt tgaaatcact gatgaggatg tggatgcggg
     6661 tcgagatccg caattagaaa aagcaattga actgctgaaa tagtcattaa tggcaatttc
     6721 ggtccaatga ttgacagttt agggcacgag ggttataatc acccgtcgcc gaaataattt
     6781 tgggagagcg tctaatggaa atcaaccccg ctgcggatac aaaccgcgca acagaaataa
     6841 agtcgaaaat gcacttcacc ggcacggtga aaaccatcac actggctggc gccgtaattg
     6901 atattggcgt cggcacgccg gctgtgctgc acatctctca gatggctgcc tctaccgagg
     6961 ggcaaccgat caagagcgtg cgcgaagtcc tgcaggaggg gcagacaatt gatgtctggg
     7021 tacgccgggt gaaagacaat cgcgttgaac tgacaatgtt ccgcccgctg gacctggaat
     7081 ggcgtgacct taaaaagggc atggtcgtca agggcaaagt tgtccgcctt gaaaagtttg
     7141 gcgcctttat tgaaattggc gctgaacggc ctggtctggt gcacatcagc gaaatggcgc
     7201 acagctatgt tcgcctgcct tcagatgttg tcaacgaggg tgatgaggtg gaagccgaag
     7261 tcattgatgt tgaccgcaag aagaagcaga tcaagctgag catgaaggcc ctgcagcccg
     7321 aaccggtgaa ggaagaagaa cctgaaatgc cggttgaaat ggcaccccgc cgtgagcgca
     7381 cagagcgtgg ggagcggggc gaacgccctg cccgccgccg cactacgagc aagggtggcc
     7441 gccgcgagca ccaggaagat aacagcgaag caatggctgc cctggccgcc gaagcggctg
     7501 ctgccgaagc cgagcccacc gcgatgggtc tggcctggca ggaagccatg gaaaaagcca
     7561 agactcgccg agaagaacgc gcccgcaagg gcaaatctgg cgccaccagc gagcatgaag
     7621 aaatcttcag ccgcacgctg gaacagaaaa gccagcagta ggatttacct tctgtaactg
     7681 ccgaaaccca agaccgctcc gccccacccg ctgagcggtc ttcgtttgtg tgtgaggcct
     7741 gagtgaattg tggtacactt tagcggttga ttcagcctga acagatcaaa ggaggagcat
     7801 gccgaccaaa tatattttct tcactggcgg ggttgtcagt tccgttggta agggcgttac
     7861 ggcggcggcg gttggccgcc tgttgaaaga acggggattt cgagtcgctg ttcagaagct
     7921 ggatccctat atcaatgtgg atccagggac gatgagcccg taccagcatg gtgaggtttt
     7981 tgtgctggac gatggcgctg aaaccgacct ggacctgggg cattacgagc ggttgattga
     8041 tatccggctg aaccgggtgt gcaatatcac caccgggcag gtctatgccg aagtcatttc
     8101 aaaagaacgg cggggagact atctgggcgg caccattcag gtgattccgc atattacaaa
     8161 tgaaatcaaa cggcggattg ggctggttgc caaaaccacc caggccgaaa ttgtgctggt
     8221 ggaagttggt ggtacggtag gcgatattga gagcctgcct tttctggaag cactgcggca
     8281 gctccgcagt gacctggggc gggaaaatac tttttatatt catgtaacct ggctgccgca
     8341 cattggcgca accaacgaac tgaagaccaa gccaactcag cactcggttc gagannnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccaag ccaaccgtca aagtggcgct
     8521 ggtgggcaag tatgttgaac tgcacgacgc ctatatcagc gtgcgcgagg cgattatgca
     8581 tgcggcgctg acggcaggcg tggaagtgga tttgcgctgg gtacattcgg cggacctgga
     8641 acgcggccag gggctggagc agttggaaga tgtggatggg attgtggttc cgggcgggtt
     8701 tggcagccgt gggattgaag ggaaaatcca ggcggcgcag caggcccgca cccgaaaaat
     8761 cccctatctg gggttatgcc tggggatgca gttgatggtg gtagagtttg cgcggtatgc
     8821 gttgggcaat gagaaagcca actcgaccga atttgaccaa tcaaccccct gcccggtgat
     8881 tgatttgatg ccggaccagc agaacatcac cgatatgggc ggcaccatgc ggctggggct
     8941 gtacccctgt aatctgcaac ctgataccat tgccgcccgg gcatatagcg agcctcaggt
     9001 gcaggagcgg caccgccatc gctttgagtt caataataat taccgacagg ttctatatga
     9061 taagggttta cgcttctcag gactctcacc tgatggtaaa ctggtagaaa ttgcggagct
     9121 ggtcgagcat cccttcatgg ttggaacgca gttccacccg gagtttctct cgcgacccaa
     9181 ccgcccccac cccctgtttg cagccttcat ggaagcggtg cgcgatcatg ctggcgtgac
     9241 agagaaatca taacaaacct gcggcagagt gcatctctgc ggcagtgaag aataatctca
     9301 ctataagagg aaaaaaaaga tggacacaat cattgaatca gttatcggac aggaagtgct
     9361 cgactcgcgc ggcaatccga ccgtggaagt ggaagttatc ctggccaatg gcgcctgggg
     9421 ccgtgcggcg gttccgtcag gcgcgtccac cggcgtgcac gaagcactgg agctgcgcga
     9481 tggcgacaag aagcgctacg gtggcaaggg cacattgcaa gctgtgcagc acgtaaacga
     9541 agatattgcc gagctaattg tgggctggga cgcagttgag caaaaagcca ttgatatggc
     9601 catgattgaa gccgatggca cgccgaacaa atccaagttt ggggccaatg ctattctggg
     9661 caccagcctg gcggtagcca aagccgcggc cgaagcgctg ggcctgccgc tgtaccgcta
     9721 cattggcggt gtctatgcgc atgttctgcc cgtaccgatg ctgaacatcc tcaacggcgg
     9781 cgcgcatacc ggctggcagt ccaccgatgc gcaggaattc atggtcatgc cgctgggcgc
     9841 cccctccttt gccgagggtc tgcgctgggg cgctgagatt tatcacgcgc tgaaggcggt
     9901 gctgaaagcc cgcggttatg ccacgctggt tggcgatgag ggcggttacg ctccggcgct
     9961 gaaggcgaat gtggaagcgg ttgaagtgat cctggaagcc attaccaagg cgggttacaa
    10021 acccggcgaa gaagtctgca ttgcgctgga cccggcagcc tcggaattgt ttgatgagga
    10081 cagcaagaag tacaacctgc gccgcgaagg caagctgctg accggcgaac agatggtggc
    10141 cttctgggcg gagtgggttc gccagttccc gattgtgtcc attgaagatg gcctggctca
    10201 ggacgactgg gacagttgga agttgatggt gcgcgagcta ggcagcaagc tccagatcgt
    10261 tggcgacgac ctgctggtca ccaacccgga acgcgtccgc cgcgccatca aagaacaggc
    10321 tgccaatgcg ctgctggtca aactgaatca gattggttcg ctgaccgaga cgattgaagc
    10381 cgtggaaacc tgccaccggg cgggctggcg ggcggtcacc tcgcaccgct cgggcgagac
    10441 ggaagattcg accattgccg acctggctgt ggcgctcaac atgggtcaga tcaagaccgg
    10501 tgcgccggca cggtcagacc gcgtggcgaa gtacaaccaa ctgctgcgga ttgaagcaga
    10561 gctgggcgaa actgcgactt atgcgggctg gtctgcgctg cgccgcggtt aattttcggc
    10621 tga
//
LOCUS       DAOPVS010000061        10375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6494_length_10375_cov_59.142539, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000061 DAOPVS010000000
VERSION     DAOPVS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6494_length_10375_cov_59.142539"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..2050
                     /gene="typA"
                     /locus_tag="PKL11_06530"
     CDS             191..2050
                     /gene="typA"
                     /locus_tag="PKL11_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNZ13297.1"
                     /translation="MQQARLDIRNVAIIAHVDHGKTTLVDQLLRQARTFRENQQLVER
                     VMDSNDLERERGITILAKNTSITIPNPKTGEMVKINIVDTPGHADFGGEVERIMNMVD
                     GVLLLVDAAEGPMPQTRFVLKKALEMGHPAVVVINKMDRKDAEPDRTLDDTFDLFIEL
                     GADDLQASFPIIYANAITGQAGTTPQLEPDLSPLFNAILDFIPPPNVDPEAPLQILVA
                     NLTYDDYRGNIAIGRIFAGSITANQQLARIGVTGQLYPERARYLYVYEGLERVEVSKA
                     LAGEIVAIAGLENVNIGETLADPENPIALPPIAVEAPTVKMTFGVNTSPFSGREGKWS
                     TSRKLRERLMAELRNNVALRVAETDTADTFIVSGRGELHLAILIETMRREGYEFQVSR
                     PEVIFQRDEAGNILEPFEEVHIEVPSEAVGIVVEMLGSRRGVMQEMLDNPDNTVHLTY
                     LCPTRGLLGFRHNFLTATRGTGIINAIFYGYQPHAGPIGGRSTHSIIVWETGVTSTYG
                     LKNAEERGLLFLGAGVDVYEGMVVGETQRSEGDIPINVCKKKHLTNMRQSNKDIEIRL
                     TPPVQMSLDEAIEYLQEDELLEVTPLTFRIRKRILNTEERGKLTKKAKEDLEQ"
     gene            2399..4171
                     /locus_tag="PKL11_06535"
     CDS             2399..4171
                     /locus_tag="PKL11_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ13298.1"
                     /translation="MSRLMMRINLTDRTVAVQPLPEKWAKFAGRGLSSSIIAAEVDPL
                     CHPLGPNNKLVFAPGLITGSAAPTSARISVGGKSPLTGGIKEANAGTPWATMAGKMRL
                     SALIIEGMPADKNVWWGIHLTWDAAAGQPKAEFFDATEYVGKDLFEVFPRVYERFGKK
                     VSIAGCGVSGEFGYGNAGIVFNDLQTGSPNRYAGRGGLGSVLGSKRVKFLVIDDHGAP
                     GIEIADKPLFDQGRQKMIDALRTHAITKPKGGLNSFGTAVLVNILNEAGGLPTRNFSS
                     GRFEGAAQIAGEAIFETNKLRLGKELYNHACSPGCIIQCSNTVYDANGKPIASCVEYE
                     SDWSLGANCGIDNLDQISEMVHLCNAYGLDTIETGTTIAVAMEAGVIPFGDGLGAIDL
                     IRKMGQGTPLGRILGAGTATTGKVYGVTRVPVVKGQSMPAYEPRAVRGIGITYATTTM
                     GADHTAGYTIAPEILSVGGAADPLSNEGKAALSRAFQATTAFIDSTGHCLFIAFAILD
                     IPSGYEGMVEEWNGVLGTHFTPADVVPLGAEILKVERAFNEAAGFTRADDRVPEFMKH
                     EPLPPHNRVFDVPDEALDSVFGDL"
     gene            4238..5425
                     /locus_tag="PKL11_06540"
     CDS             4238..5425
                     /locus_tag="PKL11_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungsten cofactor oxidoreductase radical SAM
                     maturase"
                     /protein_id="HNZ13299.1"
                     /translation="MPKITADPSGRLTLPADFLERRHMPAQAEYWLDERQGDIILHPR
                     VPDTQKLYIEATTGCNLHCRTCIRNIWGDPIALMAENTFASLIDSLPGLPDLKRVIFT
                     GFGEPLSHPRILEMIAQVRQQGLAVSVGSNGLLLTEKMARALVSLGVDRLVVSVDGVR
                     PETYSDVRGAMLSEVLDNLRRLNEIKTELNTLSPALGIEFVALKSNLSELPQLASLAM
                     HLNAARILVTNVLPYTEDMRAEMLYGYDPQPSLAAVNWPVHTGAWVTWGALELPRMHW
                     GAERRCRFVQDRSIVVGWDGSVTPCYALSHNYSYFAIDGIHKQVERYILGNVVNQSLA
                     EIWMSEDYVRFRSEVRSFHFPSCPNCDLRETCDLRQRNEGCWGWNPSCADCLWAQDIV
                     RCP"
     gene            5434..6048
                     /locus_tag="PKL11_06545"
     CDS             5434..6048
                     /locus_tag="PKL11_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNZ13300.1"
                     /translation="MKITVRLFASLAAYYPPVRASVPFELEVAPDCSLAQLSQQLNIP
                     PDELKVAFINGRIESLETCLRAGDEVGLFPPIGGGSGDISLDVWLYGDLARYGDPAAK
                     LGFAHLTITLPAGSAIRHLLAALSMPTEERGITFISGNLSAMPGIQPDLDHILQDNDR
                     VAFFHLNSMWPFQYRHGAAMVDEMRRALDEREDGGMHHAYKQGD"
     gene            complement(6054..7472)
                     /locus_tag="PKL11_06550"
     CDS             complement(6054..7472)
                     /locus_tag="PKL11_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13301.1"
                     /translation="MKPVGRLLAVIVIVLGMQACTPSLTPPTPDVLTPDTGQIITLTP
                     ETPESVETQEQTDIPSPHPIPANENIQPENLEYLGAFRLPDGNAESGWEYSGQGLTYF
                     AGGDSNGAEDGFTGSLFGIGNDQKLQVSEISIPAPIISRHLDDLNTATTLQGFTDITG
                     EMFNLEMMDLPIADLAYLPSIDGSGTGKLHFVFGQHFQEFNPSNGWHDLHLSSTEAAG
                     PWIVDSYTNYVTSDYLFDIPENWAEAIAPGQRLATGRFREGVWGGGGPALFAYNPGSE
                     AAPPVAGTKLTEVTPLLLYGVQEPGMTEIISDETMWMNDYQLADHWSGGAWLSAGDKG
                     AVIFVGTKALGQSWYGFANGVTWAYDCADHTPPTCPEVPEWPYENRGYWAEDYQAQII
                     FFNPADLAAVARGDIQTWEPQPYATLVLDDYLFNPEINPGEYKRDLVGAVAFDREHGR
                     LYVIERLADGYKSVIHVWAVNP"
     gene            complement(7489..8580)
                     /locus_tag="PKL11_06555"
     CDS             complement(7489..8580)
                     /locus_tag="PKL11_06555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNZ13302.1"
                     /translation="MISPDFEKVGIVFDKILKQQAPYSGAQVAVYHHGKLVVDLAGSR
                     PASDIITSETSFLNFSVSKAFTAAAIWRLMEEGKLDLDAPIGKYWSEFASRGKEEATI
                     RHALLHQAGVPAPHLRMQVLLWPYWGWVTRGLAREKAQFKPGTQTAYHLVNFGFILGE
                     VVRRVSGQPIDVYLQQNFFEPMGLKNTWMRIPTEALPRSPRLLAGSDKMKTTVHIFNR
                     PMIRCALIPAAGLHSNAVGTATFFQMLLNGGEYAGKRYLKPETIDLAAKSHYDGYDSY
                     IKFNMNWGLGLIIGGGAHQPPNRRDRVMGYGSTSTTFGALGLGTCMVWADRRAELVAA
                     FTCNNMLSEPEVNQRWSAISNAVWDCIPD"
     gene            8758..9639
                     /locus_tag="PKL11_06560"
     CDS             8758..9639
                     /locus_tag="PKL11_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13303.1"
                     /translation="MKQKTQTMHWIIDAALLFGFLLAFFLDLTGLAVHQWLGVALGAL
                     AVYHLFRHKNWILMTLERFFRPTSWRVRVYTLLDAGILLGFTLMVLTGLAMSTWFNLP
                     LADYDLWYNLHVASSVITLLAVVVKLALHWRWIVITFQKMVSSAGRSVGSMQPLAPQP
                     APVAHQLTLSRRQFLTTMGLVSVGAALALSNVLGNRNQVNSQSLESAPTADPTPASTA
                     TPTAQPPAAATPTSQPVAAATAQPTVVPTAVPTTQPTAYVCTYRCRKGRHCTFPGQCH
                     SYTDLNNNGYCDLGECS"
     gene            complement(9660..>10375)
                     /locus_tag="PKL11_06565"
     CDS             complement(9660..>10375)
                     /locus_tag="PKL11_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ13304.1"
                     /translation="AHLMGTHGPRYEEITEPVFSAGLEQSEEWMTAFYDDSILTFDGL
                     IEDIVTALDDAGELNNTILVIYSDHARFWKPNERIPLLIYFPDHTSVGVIRENVQNLD
                     VAPTVLDYMGWDIPEWMEGQSLIEASGLNQRMIFNEAVTAQGVEENDGFFALVPERVQ
                     PPFYQFGNVRAIVCDAYYDLNLENLTWTVLKIEGHTAPCAKNELPEPEEIKAAILQHL
                     QDHGFEITTLENLPPPPEE"
BASE COUNT         2254 a         3102 c         2572 g         2447 t
ORIGIN      
        1 gttgacctga cccgcttctt cttcggtgat gaacttgccg atcagcttga acgcagccac
       61 cagtgcactc tgccagaatg tactggcttt tggtttcttc aagtctgatg aaattccccc
      121 ctgttcaaag tgccctgctt ttgatatgat gtaacgcttg tgcgttcagc acttgaaagg
      181 gtttataaat atgcagcaag ccagactaga tattcgtaat gttgccatca tcgctcatgt
      241 cgatcatggc aaaaccactc ttgtggacca gcttctccgc caggcgcgta ccttccgcga
      301 gaaccaacag cttgtggaac gcgtgatgga ctccaatgac ctggaacgcg agcgcggcat
      361 caccattctg gctaaaaata cctccattac catccccaac cccaagaccg gcgaaatggt
      421 caaaatcaat attgttgata cccccggtca cgccgatttt ggcggcgagg ttgagcgtat
      481 catgaacatg gtggatggcg tcttgctgct ggtagatgcg gccgaaggcc ccatgcccca
      541 gacccgcttt gtgctcaaga aagccctcga aatgggtcac cccgccgtgg tcgtcatcaa
      601 taagatggat cgcaaggatg ccgaacccga ccgcaccctg gatgacacct tcgatctttt
      661 catcgaactg ggcgctgatg atctccaggc gagtttcccc atcatttacg ccaacgccat
      721 caccggccag gctggcacga ccccgcaatt ggaaccggat ctgtcgccgc tcttcaatgc
      781 catccttgat tttatccccc cgcccaatgt ggaccccgaa gctccgctgc agatcctggt
      841 tgccaatcta acctatgacg attatcgcgg caatattgcc attggtcgta tttttgccgg
      901 ttcaatcacc gccaaccagc agctggcgcg cattggcgtc accggccaac tttaccccga
      961 gcgcgcccgc tacctctatg tatatgaagg cctggaacga gtagaagtat ccaaggcctt
     1021 ggctggcgaa attgttgcca tcgccggtct cgaaaacgtc aacattggcg aaaccctcgc
     1081 tgaccccgaa aatccaattg ccctgccgcc catcgccgtc gaggccccca ccgtcaagat
     1141 gacctttggc gtcaacacct cgcccttctc cgggcgcgag ggcaagtgga gcacctctcg
     1201 caagctccgc gaacgcctga tggctgaact gcgcaacaac gtagccctgc gggttgccga
     1261 aactgacact gccgatacct ttattgtctc cggtcgcggt gaactgcacc tggctattct
     1321 gatcgaaacc atgcgccgcg agggctatga gtttcaggtc tcgcgccccg aggtcatctt
     1381 ccagcgcgat gaagccggaa atatcctgga accctttgaa gaagtccaca tcgaagttcc
     1441 ctctgaggct gttggcattg tggttgagat gctgggcagc cgccgcgggg tcatgcagga
     1501 aatgctcgat aaccccgata atacggttca cctcacctac ctttgcccta cccgcggcct
     1561 gttgggtttt cgccacaact tcctcactgc aacccgcggt acgggcatca tcaacgcaat
     1621 cttttacggt tatcaacctc atgccgggcc tatcggcggg cgttccaccc attccatcat
     1681 tgtctgggaa accggcgtta cgtcaaccta tggcctcaaa aacgctgaag agcgcggcct
     1741 gctcttcctg ggcgccggcg tggatgttta cgagggcatg gtagttggtg aaacccaacg
     1801 cagcgaaggc gatatcccga ttaacgtctg caaaaagaag cacctcacca acatgcgtca
     1861 gtccaacaaa gatatcgaaa tccgcctgac cccgcccgtg cagatgagcc tggatgaagc
     1921 cattgaatac ctgcaggaag atgagctttt ggaagtcacc cccctcactt tccgcattcg
     1981 caagcgcatt ctcaataccg aagagcgcgg caagctgact aaaaaagcca aagaagatct
     2041 ggaacagtaa aaccatcaac aaaaacccgt tgtaaaccca acgggttttt gctacaatag
     2101 tttagtggtc aattctggcc aatctgacat tccctcacag ctacatcaca gccaacagac
     2161 ctccgattcc ctgattccct ccgggataag tgaacgataa ggcctgggga gcaattccca
     2221 gtgcccgcca ttgcgcaaag gaggctctcc ccggtttttg ccgggtgagc ccatttgcgc
     2281 atttttgcgt tctcacatgc agaggaggtt gtttgagctt catatcccaa tctgtagtct
     2341 tctttccaaa ccgatatcac ctcatcccgt ttctaatctg ctgaaaggag taattgttat
     2401 gtcacgcttg atgatgcgaa tcaatctgac ggatcgaact gttgccgtgc agcccctgcc
     2461 tgaaaaatgg gccaagtttg ctggccgtgg actttcttcg tcgatcattg ccgcagaggt
     2521 ggatccgctt tgccatcccc tggggcccaa taataaactg gtgtttgccc ccggtctgat
     2581 caccggcagc gccgccccca cctcggcgcg catttcagtt ggcggcaaat ctccgctcac
     2641 cggcggcatt aaagaggcca atgcgggcac tccctgggct accatggccg ggaaaatgcg
     2701 cctctctgct ttgatcattg agggtatgcc ggccgataaa aatgtctggt ggggcattca
     2761 cctcacctgg gatgccgccg ccggtcagcc caaggccgaa ttctttgatg ctacggaata
     2821 tgtgggcaaa gacctctttg aagttttccc cagggtctat gagcgttttg gcaaaaaggt
     2881 cagcattgcc ggctgtgggg tttctggcga gtttggctat ggcaatgctg gcatcgtttt
     2941 caatgacctg caaactggct cacccaaccg ctacgccggg cgcggtggac tgggatcggt
     3001 gctgggcagc aagcgggtca aattcctggt cattgatgac cacggcgctc ccggcattga
     3061 aattgccgat aagccgctct ttgatcaggg ccgccagaag atgattgacg ccctccgcac
     3121 ccatgccatc accaaaccca aaggtggcct gaacagcttt ggcaccgccg tgctggtcaa
     3181 catcctcaac gaagctggtg gcctccccac tcgcaacttc tccagcggcc ggtttgaagg
     3241 cgccgcccag atcgccggag aagctatttt tgaaaccaac aagctccgcc tgggcaaaga
     3301 gctttataac cacgcctgca gccctggctg catcatccaa tgctctaaca ccgtttatga
     3361 tgccaatggc aaacccattg cctcctgcgt ggaatacgaa tcagactggt cactgggcgc
     3421 caactgcggc attgataacc tggatcagat ctcagagatg gttcacctct gtaatgccta
     3481 tggcctcgat accattgaaa ccggcaccac catcgctgtc gccatggagg caggtgtcat
     3541 cccctttggt gatggccttg gcgctatcga tcttatccgc aaaatggggc agggcacccc
     3601 gcttgggcgt attctgggtg ctggcacagc caccaccggc aaagtctatg gcgttacccg
     3661 ggttccagtt gttaaagggc agtccatgcc cgcctacgag ccccgcgctg tgcgcggcat
     3721 tggcatcacc tatgccacca ccaccatggg cgccgaccat acagcgggct ataccattgc
     3781 ccccgaaatt ctcagtgtgg gtggcgctgc cgacccattg agcaacgaag gcaaggctgc
     3841 tctaagccgg gctttccagg ccaccaccgc attcattgat tcaaccgggc actgcctctt
     3901 catcgccttt gccattctcg atatccccag cggttatgag ggtatggtgg aggaatggaa
     3961 cggtgtgctg ggaacacatt tcacgcctgc ggatgtcgta ccgcttggcg ccgaaatcct
     4021 gaaggtggag cgtgccttca atgaggccgc cggcttcacc cgtgccgatg accgcgtgcc
     4081 agagttcatg aagcatgaac ctctaccgcc gcacaatcgg gtctttgatg tccccgacga
     4141 ggccctcgac tcggtctttg gcgatctcta aacgcgtgta aaataaacct caggtctgcc
     4201 agccagtctc tggctggctg gcagacaggg aacccccatg cccaaaatca ccgccgaccc
     4261 atctggtcgc ctgaccctcc cggcagattt tcttgagcgc cgccacatgc ccgctcaggc
     4321 agaatactgg ctggatgaac gtcagggcga tatcatcctg catccacgtg tgcccgatac
     4381 ccaaaagctc tacatcgaag ctaccactgg ctgcaacctc cactgccgca cctgcattcg
     4441 taacatctgg ggcgatccca tcgccctgat ggcggaaaat acctttgcca gtctcatcga
     4501 cagtctgccc ggtttgcctg acctcaaacg ggttattttt accggttttg gcgagcccct
     4561 gtcacatccc cgcatcctgg agatgattgc tcaggtgcgc cagcaaggcc ttgctgtttc
     4621 agttggttcc aacggcctgt tgcttacaga aaagatggcc cgcgccctgg tatcgttggg
     4681 cgtagatcgt ctggtcgttt cagtggatgg ggtccgtccc gagacctact ccgatgtgcg
     4741 cggcgccatg ctcagcgaag tcctcgacaa tttacggcgg ttgaatgaaa tcaagaccga
     4801 actgaatacc ctctctcctg ccctgggcat cgagtttgtc gccctcaaga gcaacctgtc
     4861 tgaactgccc cagcttgcca gcctggcaat gcatctcaat gctgcccgga ttctggtcac
     4921 caatgtactg ccgtacactg aagatatgcg cgccgaaatg ttatacggct acgacccgca
     4981 gccttcgctt gcggctgtca attggccggt gcataccggc gcctgggtca cctggggcgc
     5041 tttggagctg ccccgcatgc actggggtgc agaacgccgc tgccgctttg tgcaggatcg
     5101 ctccattgtg gtcggctggg atggctcagt cacgccctgt tatgccctct cgcacaatta
     5161 cagctatttt gcgattgatg gcatccacaa acaggttgag cgttatatcc tcggcaatgt
     5221 tgtcaaccag tccctggccg aaatctggat gtccgaggac tatgtccgct tccgcagcga
     5281 agttcgcagc ttccactttc cttcctgccc caactgcgac ctgcgcgaaa cttgtgacct
     5341 gcgccagcgc aatgaaggct gctggggctg gaacccatcc tgtgccgact gtctctgggc
     5401 gcaggatatt gtccgctgtc cttaaggctg aaaatgaaaa tcaccgtccg cttattcgca
     5461 tcattagctg cctattaccc tccagttcga gcctcggttc catttgaact tgaggtagct
     5521 ccagattgct ccctggcgca gctttctcag cagctcaaca ttccgccaga cgagctcaag
     5581 gttgctttta tcaatggccg cattgaatct ttggaaacct gcctgcgcgc tggtgatgag
     5641 gtgggtttgt tcccgccgat tggcggcggc tctggtgata tctccctgga cgtctggctg
     5701 tatggcgacc tcgcccgcta tggcgacccg gcagccaaac tgggctttgc ccatctcacc
     5761 attaccttac ctgctggcag cgccatccgg catctgctgg cggcgttgtc aatgccgaca
     5821 gaagagcgtg gcattacatt tatcagtggt aacctcagcg ccatgcccgg cattcagccc
     5881 gacctggatc atattcttca ggataacgac cgcgtggcct tcttccacct caacagcatg
     5941 tggccgtttc aataccggca tggcgccgcc atggtggacg aaatgcgccg cgccctcgac
     6001 gaacgtgagg atgggggcat gcaccatgcc tataaacagg gcgattgatc cggttacggg
     6061 tttacagccc aaacgtgaat aaccgatttg taaccgtctg ccagccgttc aatcacatag
     6121 agccgcccat gttcacggtc gaaggccacg gccccgacca ggtcacgttt atattcgccc
     6181 ggattgattt ctgggttaaa tagataatca tccagaacaa gcgtggcgta tggctgcggc
     6241 tcccaggttt gaatgtcacc tcgagccact gcagccaggt cagccgggtt gaagaagatt
     6301 atttgtgctt gatagtcttc tgcccagtag ccgcggtttt catacggcca ctcaggcacc
     6361 tctgggcatg tgggtggggt atggtctgca cagtcatatg cccaggttac accattggca
     6421 aatccatacc acgattgtcc cagagctttc gttcccacaa aaatcactgc ccctttgtcc
     6481 ccggcgctga gccaggctcc ccctgaccaa tgatcagcaa gctgataatc attcatccac
     6541 atggtttcgt cagatataat ttccgtcatg ccgggctctt gtacaccata caggagcagg
     6601 ggtgtaacct cggtaagttt tgttcctgcc acagggggcg ccgcctcact cccaggatta
     6661 tatgcaaata gcgccggccc gccgccgccc cagaccccct ctcggaaacg tccggttgcc
     6721 agccgctgtc cgggggcaat agcctcagcc cagttttcgg gaatgtcaaa caagtaatca
     6781 ctggtgacgt aattcgtata gctgtcgaca atccatggcc cggcagcttc tgtgctggat
     6841 agatgcaaat catgccagcc atttgatgga ttgaactcct ggaaatgttg tccaaaaaca
     6901 aagtgtaatt tgcccgtccc actgccatcg atggacggca gatatgccag gtcggcaatt
     6961 ggcaagtcca tcatttcaag attaaacatc tcgccagtaa tatctgtgaa gccttgcagg
     7021 gtggtcgctg tattgagatc atccagatga cgtgagatga ttggcgcggg aatgctaatt
     7081 tctgaaactt gcagcttttg atcattcccg atcccaaaca acgatcccgt aaaaccatct
     7141 tctgcgccat tcgaatctcc ccctgcaaaa taggtcagcc cctgtccact atattcccac
     7201 cccgattcag catttccatc tggcaatctg aatgctccca gatattccag gttttcaggc
     7261 tgaatatttt cattggctgg gatagggtgg ggggagggaa tatctgtttg ttcttgcgtt
     7321 tcaacgcttt cgggagtttc cggtgtgagg gtgatgattt gtcctgtgtc cggggtcagt
     7381 acatcgggtg ttggaggcgt taagctcggc gtgcacgcct gcatccccaa cacaatgaca
     7441 attacagcaa gcaatcgccc cacaggcttc atggtttatt ctcaagcttt aatcaggaat
     7501 acaatcccat accgcattgg agatagccga ccaccgttga ttaacttctg gctcactgag
     7561 catgttgttg caggtgaatg ccgccaccag ttcagcccgg cggtcagccc agaccatgca
     7621 ggtgcccagg cccagcgccc caaacgtggt ggatgtgctg ccgtacccca tgacccggtc
     7681 tcgccggttg ggcggctgat gcgcgccgcc gcctatgatt aaccccagtc cccagttcat
     7741 attgaacttg atataagagt cgtagccgtc ataatgtgat ttggctgcca ggtcaatagt
     7801 ctcgggtttg aggtaacgtt ttccagcata ttcgccgccg tttaacagca tctggaagaa
     7861 tgtcgctgtt ccaactgcgt tgctgtgcag gcccgccgcc gggatcaacg cgcaccgaat
     7921 catggggcgg ttaaagatat gaaccgtggt tttcattttg tcagatccgg caagcagtcg
     7981 gggtgagcgc ggcaaagcct ctgtcggaat ccgcatccag gtattcttca agcccattgg
     8041 ctcaaagaag ttttgctgca aataaacatc aatcggctgc ccgctgacgc gccggacaac
     8101 ctcgcccaga ataaagccaa aattcaccag atgataagca gtctgggttc caggcttaaa
     8161 ctgcgccttt tcccgcgcca ggccgcgggt cacccaaccc caataaggcc acaataagac
     8221 ctgcatccgt aaatgcggcg ccggcacacc tgcttgatgc agcagggcat ggcgaatggt
     8281 ggcctcctct tttccccgtg aggcaaactc agaccaatat ttaccaatcg gtgcatccag
     8341 atccagcttg ccttcttcca tcagccgcca gatcgccgcc gccgtaaagg ccttggatac
     8401 cgaaaaattc agaaaggagg tttccgaggt gatgatatcg gaagccggtc ggctgcctgc
     8461 caggtcaacc accagtttcc cgtgatggta caccgccacc tgtgctccgc tgtaaggcgc
     8521 ttgctgttta agaatcttat caaagactat gccaactttt tcaaaatcag gggaaatcat
     8581 gatcgcctcc gcaaagcagt tttcccctcg attttatcac cacttctatc cgcagcctaa
     8641 gccatttctt cacacaatct tcacaagtca ttcacatctt tcacactgaa ttccaagcct
     8701 gtctttttat actgatgcca gaatacaaaa cgatcggatc atggatagga tcaccttatg
     8761 aaacaaaaaa cgcaaaccat gcattggatt atagatgctg ctttactgtt tggatttctc
     8821 ctcgcatttt tcctggattt gaccggcctg gctgttcatc aatggttggg cgtggccctg
     8881 ggtgcgctgg ctgtttatca tttattccgg cacaaaaact ggattctgat gacgcttgaa
     8941 cgcttcttcc gcccaacttc ctggcgggtg cgcgtctata cgctgctaga tgcgggtatt
     9001 ctgctgggtt tcactctgat ggtcttaact ggcctcgcga tgtccacctg gtttaacctg
     9061 ccgctggcag attatgacct ctggtataac ctgcatgttg catcttccgt catcacttta
     9121 ttggctgtcg tcgtaaaact agcgctgcac tggcgctgga tcgtcatcac cttccagaaa
     9181 atggtctcgt ctgcaggtcg ctccgttggc tccatgcagc cgctggcgcc ccagccagcg
     9241 ccggtcgccc atcagctgac cctctcgcgc cgtcaatttc taaccacaat ggggctcgtg
     9301 agcgttggcg cagccctggc cttatccaac gtgcttggta accgcaatca ggtcaattca
     9361 cagtccttag aatccgcccc gacagccgac cccactcccg cctcaaccgc cacccccaca
     9421 gcccaacccc ctgcggcggc cacacccacc agccaaccgg ttgcggctgc cacggcccag
     9481 cccacggtgg ttcccactgc cgtgccgacc acccaaccca ctgcttatgt ttgcacatat
     9541 cgctgccgca aaggccgcca ctgcaccttc ccgggtcaat gccacagcta taccgatttg
     9601 aacaataatg gctactgcga cctgggggaa tgcagttagc ccttctcaac cacaagaaat
     9661 cattcttccg gtgggggcgg cagattctcc agcgttgtga tctcaaagcc gtgatcttgc
     9721 aggtgctgca ggatggcggc tttgatctcc tctggctcag gcagctcatt ttttgcacac
     9781 ggcgcggtgt gcccctcaat ctttaatact gtccaggtca ggttttcaag attcagatca
     9841 taataggcat cgcaaacaat tgcccgcaca tttccaaatt gataaaacgg cggctgcact
     9901 ctttcaggta caagcgcaaa gaagccatca ttttcttcaa ccccttgcgc tgtcactgct
     9961 tcattgaaaa tcatgcgctg atttaaacca ctggcttcaa tcagagattg gccttccatc
    10021 cattcaggaa tatcccagcc catataatcc agcacagttg gcgcaacatc caggttttgc
    10081 acattttccc gaatcacgcc caccgaggta tgatccggga aataaattag tagtggaatc
    10141 cgttcattgg gcttccagaa acgggcatga tcagaataga ttaccaggat ggtgttattc
    10201 agttctccag cgtcatccag cgccgttaca atgtcttcaa tcaacccatc aaaggtcaga
    10261 atcgaatcat cgtaaaacgc agtcatccat tcttcgcttt gctccagccc cgctgaaaat
    10321 actggctcgg ttatttcttc ataccttggg ccatgggtcc ccatcaggtg ggcat
//
LOCUS       DAOPVS010000062        10367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6500_length_10367_cov_56.967126, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000062 DAOPVS010000000
VERSION     DAOPVS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6500_length_10367_cov_56.967126"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKL11_06570"
     CDS             <1..909
                     /locus_tag="PKL11_06570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13305.1"
                     /translation="ALHNRNYCAHHHEQTGFLYYFDAADDQQAANGLFDAAVGWARER
                     ELDLLLAGKGIIRSNGAGLLVEGFDDPYMPIGILYNYPYYQTLFDSYGFTKENDHLSG
                     MLLKSADIGERVHGIAERVLEQGGFSIMACKTKKEMYPWIPRVEKVHHEAFHTNAGYY
                     PTTPAEFELLARGFISLADPALIKLVMKGDEIAGFAMTFPNIAPALRAIRGRLWPLGW
                     ILVMRAQKTSKVIDGSAIGLLPQYQGRGANALLYNEIEKTLRARNVERVNIIQVDERN
                     ARSKADHETMGVKFHLRHRTYRLPIG"
     gene            915..2351
                     /locus_tag="PKL11_06575"
     CDS             915..2351
                     /locus_tag="PKL11_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HNZ13306.1"
                     /translation="MRNAAAAPLTRLQKLIYGSGDWSKASFNVLRQLFYAIFLTDVVG
                     LDARLASLAALISIVWDAVNDPIIGALSDNVRTRWGRRRPFILLFAVPFALAFVLLWW
                     APPWQSQVMLMIHVTLAYMVSDTLQTLVTVPYLSLTPELAKDYDERTSLTSYRMFFNL
                     LASLATAVAAPGILDAVVKAGYSQQQGYVTVGALFGGLAVLPLLLIFFSLREQDAPPQ
                     PPEEQLTFRKTIRVLWANRPFRFAAGFYVLNWISFDVVGLMLPYYLLYWVGEGNLLAK
                     VNLLGVRLPLESAIFGVLLITATAAIPLWGWLARRFSKRFAYIAGMGFWIGVQLLIYS
                     LQPQQTTLMLMLAALAGLSVSTAHVLPEAIFPDVIDWDELRTHTRREGMYYGAINFIR
                     KLSSALATFITLQFLGWFGYQAPPADAQVFTQSETTLQVIRLLTGPFVAVLLIGAIGF
                     AWFYPLSRERQKRIRRALERRYLFVKEG"
     gene            2356..3198
                     /locus_tag="PKL11_06580"
     CDS             2356..3198
                     /locus_tag="PKL11_06580"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNZ13307.1"
                     /translation="MFTTFITADELTKHLNQPDWVLVDCRTDLMKPEWGREDYRRAHV
                     PGAVYADLNRDLSGPTGPQTGRHPLPEPGAFRATMGKLGIDASKQVVVIDTAAGAFAA
                     RLWWMLRLYGHAAVAVLAGGFPLWEREGRPTRSGEETNPITQFNGEPQTGWFLTAEEV
                     NRVRDDSSWLVVDARTPERYRGEQEPIDPVAGRIPGAVNRAHTLNLQAGGLPLPPETL
                     REQFTALLGSTPPERTVFYCGSGVTSAFHLLAMAYAGMPDARLYPGSWSEWIRDPNRP
                     IGKG"
     gene            complement(3279..4316)
                     /locus_tag="PKL11_06585"
     CDS             complement(3279..4316)
                     /locus_tag="PKL11_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNZ13308.1"
                     /translation="MATQAAQQLNQAGLSRADYKGAPSTLCQGCGHNGIASQITSALY
                     ELNVVPETVVKFSGIGCSSKSPTYFLSRSFAFNGVHGRMPSLALGALFGDARLHGIGV
                     SGDGDTASIGMGQFKHIVRRNMPMVYIVENNGVYGLTKGQFSATAELGLSLKRQGTNP
                     FLPVDICLEALASNASFVARSFAGDPRQLKELLKAAYSHRGIAVIDIISPCVTFHNQD
                     GWLHSYTYGKEHEEPLHEISYVPARDEVTVEDFEEGTTRDVELHDGAVIRLKKLERDY
                     DPTNRAAAFQILEEANQSGELVTGLIYVNPEIPSMFDAYNLPEEGLNRLPAHKLRPTP
                     ESLDRLNASFA"
     gene            complement(4318..6159)
                     /locus_tag="PKL11_06590"
     CDS             complement(4318..6159)
                     /locus_tag="PKL11_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNZ13309.1"
                     /translation="MAEETPAPASPARKPIVNDFCITFSTINGSGSATANNILLRAFF
                     RMGIPVSGKNIFPSNIQGQPTWYSLRLSKDGYLARLERDDIVVAMNPATIAKEVGFIV
                     PGGVLIYADDIKLTELRPDIIAYPMPVKQLVKESEVAPGLRDYIANMVYVGVVGYLLK
                     MPLDVLRQTIEVHFKGKQKAIDSNFGTVRAAYEWAAANLTKTDPYEVTPMDRTKGYIM
                     TDGNTAGALGSLYGGMQFCGWYPITPASSLVESFTDYAAPLRTDPETGKQTFAVVQAE
                     DELAAAGMIVGAGFAGLRAMTATSGPGLSLMAEYLSLAYYAEVPLVIWDVQRVGPSTG
                     LPTRTAQCDITEAYFIGHGDTQFILLFPGSVQECFEFGWRALDYAERYQTPVLVMSDL
                     DLGMNYWMTPAFEYPDQPMDRGKVLWEEDLEKMIKERNGDWGRYLDIDNDGIPYRTVA
                     GNRHPRSAYFARGTGHDEYARYSEEPDVWERVLNRIAKKFETARNQLVKPVIQSRQGA
                     RIGILSVGSTDPAIIEALDCLQADHNLPADYLRVRSIPFSDEVYDFIRNHDRIYVVEM
                     NRDGQLHQILRLNIPDCTSKLISVAHIDGMPLTAKWICDQITTQEAK"
     gene            complement(6208..6906)
                     /locus_tag="PKL11_06595"
     CDS             complement(6208..6906)
                     /locus_tag="PKL11_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNZ13310.1"
                     /translation="MLRERNVLEQSAFLQYLTERTDAEGDRLPPLSELSQQLGVSIAS
                     LREQLEVARTLGVVEVRPKVGIRRLPYRFKPAVLTSLGYAVSLDTRSFRAFSDLRNHL
                     EMSYYMAAVSLLKLEDHAELRALVQSAQARLNRPTPQIPHAEHRDLHLLIYRRLNNPF
                     VTGLLEAYWDMYEAIGLNVYTDMVYLKRVWEYHDRMVEAICVGDFATGYQALIDHMNL
                     IYERSKSDGAQKFE"
     gene            complement(7138..9588)
                     /gene="leuS"
                     /locus_tag="PKL11_06600"
     CDS             complement(7138..9588)
                     /gene="leuS"
                     /locus_tag="PKL11_06600"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNZ13311.1"
                     /translation="MSEQIYNPAEIEARWQARWAADRLYEADVDPARPKHYALTMLPY
                     PSGDLHIGHWYAMTPSDAQARFMRMQGYNVLFPMGFDAFGLPAENAAIRDNIHPRIRT
                     MANIERMRKQMRRMGAMFDWRREIVTCDPAYYRWTQWFFVQFYKNGLAYRKHSPVDWC
                     PKCNTTLAREQVWGDDRHCERCGTPVIKKNLDQWFFRTTRYAEELLSYEGLDWPETVR
                     VLQTNWIGRSNGAHVTFTTETGSPLTVFTTRPDTLWGVTFMVLAPEHPLVAEITSEAQ
                     RPLVQAYIEQATRQTDVQREAADKEKTGVFTGGYAINPVNGERVPIWIADYVLMTYGT
                     GAIMAVPAHDQRDFEFARKFNLPIRIVIQPSDLEPIRPEDLTAAVPASGDMVNSGPLT
                     GTPGAEAFQRAVGYLEARGLGNGAVNYKLRDWLISRQRYWGAPIPIVYCPACGPVAVP
                     ESQLPVLLPDDVEWKPTGESPLKLHPTWRFTTCPQCGGPAERETDTMDTFMCSSWYHL
                     GYLNKGMSDQYPFPPEEYDYWMPVDTYTGGIEHATMHLIYTRFFHKACRDVGITEGHS
                     GMLRLRNQGMVLGEDGEKMSKSRGNVIAPDDLVDAYGADTIRAFLMFFCRWEMGGPWS
                     SGGIEGTSRWLRRVWSAVIDPAPMAAPDPVLVRQLRRKVHQTLRSVTRDYQAFEFNTI
                     VSSLMELMNEMLRVKSAGLWNSPAWNEAVELYLKMLAPICPHISEELWARLGKPYSIH
                     NQLWPAVDEAAAAEDEITLVVQVNGKVRDRITLPADVTEEAAKAAALSSEVIQKHLEG
                     KPPRQVVYVRGRLVNIVV"
     gene            complement(9917..9989)
                     /locus_tag="PKL11_06605"
     tRNA            complement(9917..9989)
                     /locus_tag="PKL11_06605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9954..9956),aa:Ala,seq:ggc)
     gene            complement(10037..10109)
                     /locus_tag="PKL11_06610"
     tRNA            complement(10037..10109)
                     /locus_tag="PKL11_06610"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10074..10076),aa:Ala,seq:cgc)
BASE COUNT         2109 a         2715 c         3321 g         2222 t
ORIGIN      
        1 gcgctgcata accgcaacta ctgcgcccac caccatgaac aaaccggatt tctttattat
       61 tttgatgcgg ccgatgacca gcaggctgcc aatgggctgt ttgatgcggc ggtgggctgg
      121 gcacgcgagc gcgagttgga cttgctgctg gcgggtaaag gcatcatccg atccaatgga
      181 gcggggctgc tagtggaagg ttttgatgac ccgtatatgc cgattgggat tttatacaat
      241 tacccttatt atcagaccct gtttgacagc tatggcttta ccaaagaaaa cgatcatttg
      301 tcgggcatgc tgctgaaaag cgccgatatt ggggagcgcg tccacggaat cgctgaacgc
      361 gtgctcgaac agggtggctt ttctattatg gcctgcaaga ccaaaaaaga aatgtatccc
      421 tggattccgc gggtggagaa ggtgcatcac gaggcctttc ataccaatgc aggctattat
      481 cccactacac cggctgaatt tgaactgctg gcgcgcgggt tcatctcgct ggcagacccg
      541 gcattgatca aactggtgat gaagggcgat gaaattgccg gttttgcgat gacattcccc
      601 aatattgcgc cggcgctgcg ggccattcga gggaggctgt ggccgctggg gtggattctg
      661 gtaatgcggg cacagaagac ttctaaagtg attgacggaa gcgcaattgg gctgctgccg
      721 cagtaccagg ggcgaggcgc caacgccctg ctgtacaatg aaattgaaaa gacgctgcgc
      781 gcccgcaatg ttgagcgggt aaacattatc caggtggatg agcgcaatgc ccgcagcaag
      841 gcagaccacg aaaccatggg cgtcaaattc catctgcggc accgcactta ccgcctgccg
      901 attgggtgag ccagatgcgt aacgctgccg cagcaccgct gacacggctg caaaagctga
      961 tctacggctc cggcgactgg agcaaggcca gcttcaacgt actgcggcag ctattctacg
     1021 ccatctttct gacggatgtg gtggggctgg atgcgcggct ggcttcacta gcggcactga
     1081 tcagcattgt atgggatgcg gtcaacgatc caattattgg ggcgctgagc gataatgtgc
     1141 gtacgcgctg ggggcgacgg cggccattca ttttgctgtt tgcggtgccg tttgcgctgg
     1201 cctttgtgct gttgtggtgg gcgccgccct ggcagagcca ggtcatgctg atgatccatg
     1261 tgacgctggc ctatatggtc agcgacacac tccagaccct ggtcacggtg ccatatcttt
     1321 cgctgactcc ggaactggca aaggattatg atgagcgcac ctctctgacc agctaccgga
     1381 tgttctttaa tctgctggct tccctggcaa cggctgtggc ggcaccgggg attctggatg
     1441 cggtggttaa agccgggtac agccaacagc agggctatgt gacagtgggg gcgctgtttg
     1501 gcggactggc agtgctgccg ctgctgctga tcttcttcag cctgcgcgaa caagatgctc
     1561 cgcctcaacc gcccgaggag cagcttacat ttcgtaaaac catccgggtg ctgtgggcta
     1621 accggccgtt ccgatttgcg gcgggtttct acgtgctgaa ctggatttca tttgatgtgg
     1681 tggggctgat gctgccgtat tatctgctct actgggtggg cgagggcaac ctactggcca
     1741 aggtaaacct cttgggtgtg aggctgcccc tggaatcggc aatctttggg gtgttgttga
     1801 ttacggccac ggcagccata ccgctgtggg gctggctggc gcggcggttc agcaagcggt
     1861 ttgcttacat tgccgggatg ggcttctgga ttggggtgca gttgttgatt tattcgctgc
     1921 agcctcagca aaccaccctg atgctgatgc tggcggcgct ggcagggttg agtgtttcca
     1981 ccgcacatgt tctgcccgag gcaattttcc cggatgtgat tgactgggat gagctgcgta
     2041 cgcatacccg ccgcgagggc atgtattacg gcgccatcaa tttcatccgc aagttgagca
     2101 gtgcgctggc gactttcatc acgctgcaat ttctgggctg gtttggttac caggcgcctc
     2161 cggcagatgc gcaagtcttc acccaatcag agacgaccct gcaggtcatc cggctgctga
     2221 cggggccgtt tgtggccgtg ctgctgattg gcgcgattgg gtttgcctgg ttctacccac
     2281 tctcacgcga acggcagaaa cggattcgcc gggcgctgga acgccgatac ctgtttgtaa
     2341 aggaaggata ggccaatgtt cacgacattt atcacggctg acgaactgac taaacacctg
     2401 aatcaacccg actgggtgct ggtggactgc cgaactgatt tgatgaagcc tgaatggggc
     2461 cgggaagatt accggcgagc ccatgttccg ggtgcggtct atgccgacct gaaccgcgac
     2521 ctgtctgggc caaccggccc acaaaccgga cgccacccct taccagagcc aggggcgttc
     2581 cgcgcaacca tggggaagct ggggattgac gccagcaaac aggtggtggt gattgatacc
     2641 gccgcgggtg cttttgccgc ccggttgtgg tggatgctgc ggctgtatgg acatgcggcg
     2701 gtggcggtgc tggcgggggg atttccgctg tgggagcggg agggccgccc cacccggagc
     2761 ggcgaagaga ccaacccaat cactcagttc aacggcgaac cgcagaccgg gtggttcttg
     2821 acagccgaag aagtcaatcg tgtgcgcgac gattcgagct ggctggtggt ggatgcgcgc
     2881 acacccgagc gctaccgggg cgagcaagaa ccgattgacc cggtggcggg gagaattccg
     2941 ggtgcggtta accgggcgca cacgctgaac ttgcaggcgg gcggtctgcc gctgccacca
     3001 gaaacgctgc gcgagcagtt tacagcactg ctgggttcca ccccgccgga gagaactgtt
     3061 ttctactgcg ggtcgggggt gacctcggct tttcacctgc tggcaatggc ctatgccgga
     3121 atgcctgatg ccaggctgta tccgggttcg tggagtgagt ggattcgcga ccccaaccgg
     3181 cccatcggaa agggctgata gaggcgtttt tcgggtcgaa tagaaaccgc ccgggcaaat
     3241 atatccgggc ggtttgtctt caggctatgg gcgttggatc aggcgaatga ggcgttgagg
     3301 cgatccaacg actcgggggt ggggcgcagt ttgtgggctg gcaagcggtt gaggccttcc
     3361 tcgggcaagt tgtaggcatc gaacatggag ggaatttctg gattgacata gatcaaaccg
     3421 gtgaccagtt cgccgctctg gttggcttct tccaaaatct ggaaggcagc agcgcggttg
     3481 gtggggtcgt aatcgcgttc cagtttcttg aggcgaatca cagcgccatc gtggagttcc
     3541 acatcgcgcg tggtgccttc ttcaaaatcc tcaaccgtga cttcatcacg ggcggggacg
     3601 taagaaatct cgtgcagggg ttcttcgtgc tctttgccgt aggtatagga atgcagccag
     3661 ccatcctggt tgtggaaggt gacgcagggg ctgatgatat caatcacggc aatgccgcgg
     3721 tggctgtagg ccgctttgag cagttctttg agctggcgag gatcgcccgc aaaggagcgt
     3781 gccacaaaag aggcgtttga agccagtgct tccaggcaga tatccaccgg caggaagggg
     3841 ttggtgccct ggcgtttgag ggagaggcca agctctgctg tggcagagaa ctgacctttg
     3901 gtcaggccat agacgccatt gttctcgaca atataaacca tgggcatatt gcggcgaacg
     3961 atgtgcttga actgccccat gccaatgctg gcggtatcgc catcaccgct gacgccaatg
     4021 ccatgcagcc gggcgtcgcc gaacagcgcg ccgagggcca gcgaaggcat gcgcccgtga
     4081 acaccattga aggcgaatga gcggctgagg aagtaggtgg ggcttttgct ggagcagcca
     4141 atgccgctga actttaccac tgtttcggga acaacattca attcgtagag tgcagaggtg
     4201 atctggctgg cgatgccatt gtgaccgcag ccctggcaga gggtgctggg ggcgccttta
     4261 taatcggcac gggaaagacc ggcctggttg agttgttgag cagcttgagt cgccatgtta
     4321 cttcgcctcc tgcgtggtga tttgatcaca gatccatttg gcggtcaaag gcataccgtc
     4381 aatatgggca acagaaatca atttggatgt gcaatcgggg atattgagcc gcaaaatctg
     4441 gtgcagttga ccatcgcggt tcatttccac aacatagata cggtcgtggt tgcggatgaa
     4501 gtcgtagact tcatcagaga aggggatcga gcgcacccgc aggtaatcgg cgggcaggtt
     4561 atgatccgcc tggaggcagt cgagcgcttc gataatcgcc gggtcggtag agcctaccga
     4621 gaggatgcca atgcgggcgc cctggcggct ctggatgacg ggtttgacca gttgattgcg
     4681 ggcggtttcg aattttttgg cgatgcggtt gaggacgcgt tcccagacat cgggctcttc
     4741 gctgtagcgg gcatattcat catgtccggt accacgcgca aagtaggcgc tgcgggggtg
     4801 gcggttgcca gccacggtgc ggtaggggat gccatcgtta tcaatatcca ggtagcggcc
     4861 ccaatcgccg ttgcgttctt tgatcatttt ctcaagatct tcttcccaca gcactttgcc
     4921 acgatccatg ggttgatcgg ggtattcaaa ggcgggggtc atccagtaat tcatgcccag
     4981 gtcgaggtcc gacatgacga ggacgggggt ctggtagcgt tcggcatagt cgagggcgcg
     5041 ccagccgaac tcgaaacatt cctggacgga gccggggaag agcaggatga actgggtatc
     5101 gccatggcca atgaagtagg cctcggtgat atcgcactgg gcggtgcggg tgggcaggcc
     5161 cgtgctgggg ccaacccgct gtacatccca gatgacgagc ggaacctcgg catagtaggc
     5221 caggctgagg tactcagcca tcaggctgag gcccgggccg gaggtggcgg tcatggcgcg
     5281 caggccagca aagccagcgc ccacaatcat gccggcggca gccagttcat cttcggcctg
     5341 aaccacggca aaggtttgct tgccggtttc ggggtcagtg cggagagggg cagcatagtc
     5401 ggtgaaggat tcaaccaggg atgaggccgg ggtaatgggg taccagccgc agaactgcat
     5461 accgccatac agagaaccca gcgcaccggc ggtattgcca tcggtcatga tgtagccctt
     5521 ggtacgatcc atgggggtta cttcgtaggg gtcggttttg gtcaggttgg cggcagccca
     5581 ttcataggcg gcgcgcacgg tgccgaagtt ggagtcaatg gccttctgct tgcccttgaa
     5641 gtggacctca atggtctggc gtagtacgtc caggggcatc ttcagcagat agcccactac
     5701 acctacatag accatgttgg cgatatagtc gcgcaagccg ggagcgactt cggactcttt
     5761 gacaagctgc ttgaccggca tggggtaggc aataatatcc gggcgcagct cggtcagttt
     5821 gatgtcatcg gcataaatca gcacaccacc ggggacaata aagccaactt ctttggcaat
     5881 ggtggcaggg ttcatggcga ccacaatgtc gtcacgttcc aggcgggcca ggtagccgtc
     5941 tttgctcagg cgcagcgaat accaggtggg ctgcccctgg atattggaag ggaagatatt
     6001 tttccccgat accgggatgc ccatgcggaa aaatgcgcgc aggaggatat tatttgccgt
     6061 agcgctcccc gagccattga tcgtgctaaa agtaatgcaa aagtcgttga cgatcggctt
     6121 acgggccggg ctggcaggtg caggggtttc ttcagccata gctctcctca ataggtgtac
     6181 tgaacagtga tggtaaatat tcgggggcta ctcaaatttt tgagcgccgt ccgattttga
     6241 acgttcgtaa atcaggttca tatgatcgat cagagcctga taaccggtgg cgaaatcgcc
     6301 aacgcaaatc gcttcaacca tgcgatcatg gtattcccag acacgcttga gatagaccat
     6361 gtcagtatag acgttcaggc cgattgcctc atacatatcc cagtaggctt ccagcaggcc
     6421 ggtgacaaag ggattgttga ggcggcggta gatgagcaag tgcaggtcgc gatgttcagc
     6481 gtgggggatt tgcggcgtgg ggcggttgag ccgggcctga gcgctttgga ccagcgctcg
     6541 cagttcggcg tgatcttcga gcttgagcag gctgaccgcc gccatataat aggacatttc
     6601 caggtggttg cgcagatcag agaaagcgcg gaaagacctg gtatccagcg agacggcata
     6661 acccaggctg gtgagcacgg cgggcttaaa gcggtagggc aggcggcgga tgccgacctt
     6721 gggacgcact tccaccaccc ccagcgtccg ggcgacttcg agctgttcac gcaagctggc
     6781 aatactgacc ccaagctgct ggcttaactc agaaagcggc ggcagcctat ccccctccgc
     6841 atcggtccgc tccgtcagat attgcaaaaa agctgattgt tccagcacgt tacgctcacg
     6901 cagcatggcg agcagcctct ttttgattga ttaatcagat tattacgatg attggagtat
     6961 agcacgcggg atagggattg tcaatcagga tgaatgtcac gattgcgggc gaggtttgag
     7021 attgggaaga ccccgctcta cccaaagagg cgaaccttac tggttcgcct cttgacagca
     7081 tgggttttct attctgtttg ggcccgggcg agcatgtgca ccatccgggc atcggaatta
     7141 cacaacgatg ttaaccaggc ggccgcggac atagacaacc tggcgcggag gcttgccttc
     7201 cagatgtttc tggatcacct cgctggacag ggcggcggct ttggcggctt cttcggttac
     7261 atcggcgggc agggtgatgc ggtcacgcac tttgccattg acctggacga ccagggtaat
     7321 ctcatcttca gcagcagcag cctcatccac agcgggccag agctggttgt ggatggaata
     7381 gggcttgccc aggcgtgccc acagctcttc agagatgtgc gggcagatgg gcgccaacat
     7441 tttcaagtag agctcaacgg cttcgttcca agcagggctg ttccacagac cggctgattt
     7501 gacgcgcagc atttcgttca tcagctccat caaggatgag acgatcgtgt tgaactcaaa
     7561 ggcctgatag tcgcgtgtga cggagcgcag ggtttgatga accttgcggc gcaactggcg
     7621 caccagaacg ggatcgggcg cggccatggg agcagggtca atgacagccg accagacccg
     7681 gcgcagccag cgagaggtgc cctcaatgcc gccgctggac cagggaccgc ccatttccca
     7741 gcggcagaag aacatcagga aggcgcggat ggtgtcggca ccataggcat ccaccagatc
     7801 atcgggagcg atcacgttgc cgcggctctt ggacattttt tcgccgtctt ctcccagcac
     7861 catgccctga ttgcgcaggc gcagcatgcc ggagtggccc tcggtgatgc caacatcacg
     7921 gcaggctttg tggaagaagc gggtgtaaat caggtgcatg gtggcatgtt caatgccgcc
     7981 ggtgtaggta tctacaggca tccagtagtc atattcttcc ggcgggaagg ggtactggtc
     8041 gctcatgcct ttgttcaaat agcccaggtg ataccaggac gagcacatga aggtatccat
     8101 ggtgtcggtt tcgcgctcgg cagggccacc gcactgtggg caggtggtaa accgccaggt
     8161 ggggtgcagc ttgagggggc tttcgccagt gggcttccat tccacatcat cgggcagcag
     8221 aacaggcaac tggctttcgg gcacagccac cgggccgcag gccgggcagt agacgatcgg
     8281 gatgggggcg ccccagtagc gctgacggga gatcaaccag tcacgcagct tgtagttaac
     8341 cgcaccgttg cccaatccac gcgcttccag gtagcctacg gcgcgctgga aggcctcagc
     8401 gccgggggta ccggtgaggg ggccagaatt gaccatgtcg cccgaggcag gcacggcggc
     8461 agtcagatct tccgggcgaa tgggttccag gtcagacggc tggataacga tccgaatggg
     8521 caggttgaat ttgcgggcaa attcaaagtc gcgctggtcg tgggcaggga cagccatgat
     8581 ggcgcccgtg ccgtaggtca tcaagacgta atcggcaatc cagatgggca cccgctcacc
     8641 attgaccggg ttgatggcgt agccgccggt gaagacgccg gttttttctt tgtcggcagc
     8701 ctcacgctgc acatcggtct ggcgggtggc ctgttcgatg taggcctgca ccagagggcg
     8761 ctgcgcctcg gaggtaatct cagcaaccag gggatgttcc ggcgccagga ccatgaaggt
     8821 gacaccccac aacgtatcgg ggcgggtggt gaagacggtc agcggggagc cggtttccgt
     8881 ggtaaaggtc acatgggcgc cattggagcg gccaatccag ttggtttgca gcacgcgcac
     8941 cgtttcgggc caatccaggc cttcgtaaga gagcaattct tcggcgtagc gggtggtgcg
     9001 gaagaaccat tggtccaggt ttttcttgat gaccggggtg ccgcagcgct cgcagtgacg
     9061 gtcatcgccc cagacctgct cgcgggccag ggtggtgttg cacttggggc accagtccac
     9121 cggggagtgt ttgcggtaag ccagcccgtt cttgtagaac tggacgaaga accactgcgt
     9181 ccagcggtaa taagccgggt cgcaggtgac gatctcgcgc cgccagtcaa acatggcgcc
     9241 catccggcgc atttgcttgc gcatgcgctc gatgtttgcc atggtgcgga tgcgggggtg
     9301 aatattatca cggatggcgg cgttttcggc aggcaagccg aaggcgtcaa accccatggg
     9361 aaacaggaca ttgtagccct gcatgcgcat gaagcgcgcc tgggcatctg agggggtcat
     9421 ggcgtaccag tggccgatat ggaggtctcc agagggatag ggaagcatgg tcagggcgta
     9481 atgtttggga cgggcgggat ccacgtcagc ctcgtagagg cgatcagcgg cccagcgggc
     9541 ctgccatctg gcttcaattt ctgcgggatt gtagatttgt tcgctcatgc agcacctccg
     9601 aaaaagaaaa gaatgttggt ttggggttcc gggcggagaa acaaaaaagc cctcgtccag
     9661 acagggacga aggcatgctc cgcggtacca ccctgattgc cggttcgcac acagcttgcc
     9721 ggccgctcag aacgcgctaa cgggcgaacc cgttgccggt tactggtttt caccggcaca
     9781 gcctgcttct tgaaaaagaa gcgcagcagg cgagttcgat ctggctgatg ctccgttggg
     9841 catcgtgtcg ggcttccacc ttgctctccc gactcgctga cggatgatct actactcctg
     9901 ctctggcttt tgtttgtgga cccagaggga ttcgaaccct cggccttctc aatgccattg
     9961 agacgcgctc ccaactgcgc tatgggccca tataaactgt tccgtgcttg taaagtacag
    10021 aggggtcaat ccccagtgga cctgagggga gtcgaacccc tggcctcctc agtgcgattg
    10081 aggcgcgctc ccaactgcgc tacaggcccg tttgatttga gtaagccgat tttacccgag
    10141 agactgggga ttgtcaagaa cggattttgg aaaaatttga cgatgttttg acgcctgtct
    10201 gtcaaaatat gtctataatc atattattcc cctgtgattg ccaaaggagt tgattatggg
    10261 caccattggt cgagaagttg ggcgcggctg cggacgatcg attatcgttt acattattgc
    10321 cgggattgtg atgctctgcc tgtttggcgg gctggtgttg gttccgc
//
LOCUS       DAOPVS010000063        10365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6504_length_10365_cov_60.177692, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000063 DAOPVS010000000
VERSION     DAOPVS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_10365_cov_60.177692"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..191)
                     /gene="rpmB"
                     /locus_tag="PKL11_06615"
     CDS             complement(9..191)
                     /gene="rpmB"
                     /locus_tag="PKL11_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNZ13312.1"
                     /translation="MAKCDNCGKTTTFGHNRSFSQRATNRTFHPNLQRVSVFVNGRKV
                     RKVLCAKCIRSMAKSA"
     gene            324..680
                     /locus_tag="PKL11_06620"
     CDS             324..680
                     /locus_tag="PKL11_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNZ13313.1"
                     /translation="MSEPTNPFGTIFISKRAITTIAAQSALESYGVVGLAAKNLADSL
                     AQALVKDPALGVTVHYDGGQVQIDLYLIIEYGLRVTTVANSVIESVRYHIEKATGLVV
                     KQVNVHVRGLRISNPD"
     gene            706..>1643
                     /locus_tag="PKL11_06625"
     CDS             706..>1643
                     /locus_tag="PKL11_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNZ13314.1"
                     /translation="MSTAEFYSVDRQQLAQATTLDGQGLKTLVEAALTWLKTNQQTVN
                     ALNVFPVPDGDTGTNMLLTLQAAYNEVANSGERNVGKMAHAIAQGALMGARGNSGVIL
                     SQIWRGFARGLDSLDVMDAAIMVRALIESRNTAYKGVVRPVEGTILTVIKDVSIAAEQ
                     ALAETQDLNLLLEQIVQAADVSVKHTPELLPILKQAGVVDSGGKGLFFIFEGMLRYLK
                     DQPLETATTTVMPLAAMQLEETMEAVEAGQDFEVVVDFRPNEPLNLESFYAELSEMGT
                     SIQVGEGDGFYRMHIHVPTENRYLPIDYTMKIGAVT"
     assembly_gap    1644..1743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1744..2226
                     /locus_tag="PKL11_06630"
     CDS             <1744..2226
                     /locus_tag="PKL11_06630"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13315.1"
                     /translation="KNIVLAAQAAASMTVKKVAVIPSKSVPQGLSAMLRLNPEGDFDE
                     VVAEMNEAITEVESGEITVATRSIEINGVSVKEGEVIALLNGQLIAAQPDLEKACLAL
                     LEKANTADRERITFFYGQNIGRAEVNRIVDIIRTAYPTHEIELHEGGQPHYQFILSIE
                     "
     gene            2229..3077
                     /locus_tag="PKL11_06635"
     CDS             2229..3077
                     /locus_tag="PKL11_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ13316.1"
                     /translation="MSSICILTDSSAQFPQPTFPGQNMVRMISLGVELAGKTYTDSKE
                     IRLNTLPAAATPALHPRLLPPSVEQFQQMFMDLSSQHDAVLVILLSEQLHSTCSNAMQ
                     AASALQSCTNVQVINSQTTSVGLGLLVQTAAEAIAGGASPAHAERLVRSMIPHVYTII
                     CSQGLSYLHYSGFLDHAQARIGEMLGLLPLFTLEEGKLTPLEKVRSLRQVLDFFQEFL
                     SEFDHLRHIALLQGTLGFGSEARLLRQFAQENYAKTPYSEHTFNTPLASLLGPRSLCL
                     IAAEER"
     gene            3107..5557
                     /gene="recG"
                     /locus_tag="PKL11_06640"
     CDS             3107..5557
                     /gene="recG"
                     /locus_tag="PKL11_06640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNZ13317.1"
                     /translation="MLSAIEKLLKFMRLEVDRGYDNRAVVGGLNKVLPWWEPEARASG
                     MDAGLLERVVEQLNAYPGYSLEDRAACLNDLLNELEAALPAETERRTATPPHPRPVDS
                     DAQSAPSERSRRPAVEREPSAPIGLDAPVTVLPGVGPKYAQTMKQLGLNTLADLLYYF
                     PRRYDDYSQLKTINRLEYGEELTIIATVQSVATRPLRGGQSSMIEAVVSDGTGSIRIT
                     WFNQPWIANRLNRNDQIAISGKVDMYLGRLVMNSPDWEPIEQNSLNTNRIVPVYPLTA
                     HVTQKWLRRLMYDTVTYWAARITDYLPEDIRRAVDLPPLGPALVQAHFPDSMERLRAA
                     RARLAFDEILLLQLGVLQQKSNWQSVAARVFETPDAWLDAQVQRLPYPLTGAQQRALA
                     HVRADLSSGRPMNRLLQGDVGSGKTVIAALGAAMVTRAGAQAAIMAPTSILAEQHYRN
                     LTRMLTEPGDNDNAALKPEEIRLLVGDTPEAEKAEIRTGLEEGNIKLIIGTHALIEAP
                     ISFNQLEFVVIDEQHRFGVAQRAALRVKGQTPHLLVMTATPIPRSLALTLYGDLDLTV
                     MDEMPAGRQPVETHVLHPLERERAYQLIRTQVQQGRQAFIIYPLIEKGDKEESKAAVD
                     EHERLQSVIFPRLKLGLLHGRMRPDEKEQVMARFRDGEFQILVSTTVVEVGVDVPNAT
                     VMLIEGANRFGLAQLHQLRGRVGRGGDQAYCLLIPENENAIENERLAVMAETNDGFVL
                     AERDLDQRGPGDFLGTRQAGFAQLRMASLTDVRLIEKAREQAMKINDRDPDLSAPENA
                     ALRRTLQRFWNPGKGDIS"
     assembly_gap    5563..5662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5663..5851
                     /locus_tag="PKL11_06645"
     CDS             <5663..5851
                     /locus_tag="PKL11_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13318.1"
                     /translation="DIEVMALITNEEHTFLSSTTVREIASLGGDVSSMVPPFVAKALY
                     QRFGEIGENHQHMTSLRD"
     gene            5908..6372
                     /locus_tag="PKL11_06650"
     CDS             5908..6372
                     /locus_tag="PKL11_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13319.1"
                     /translation="MDILHLVDRLEEVFNEGRSIWFTRQVAVDEDRMLDLIDQMRISI
                     PEEIKKAQQITAQRDRMLAQAQEEANRTVQQARERAQQMIERDDVTKEARVQAEQILA
                     AARDEAEITRRDADAYVLKSLTDMEMELERLLTQVRNGIHTIKETQNPTNEA"
     gene            complement(6379..8874)
                     /gene="lon"
                     /locus_tag="PKL11_06655"
     CDS             complement(6379..8874)
                     /gene="lon"
                     /locus_tag="PKL11_06655"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNZ13320.1"
                     /translation="MKDNQRASRPDDSEDYISALHQRTEELYQVPDMTVNEDGTIEAG
                     VLILRDVVVFPHMVSPIFVPPGPNLLAIEDAQEAYETMIALIVSDPDAEAEPTQKDFL
                     PIGVEVAVGRLLSMPDDNNSALIQGRRRLEVLEFTQTEPYFRVRARVIVESTHVDRQT
                     EALMRTSRDLFERCVQLDRSLPEEAHLFSLNIQEPGWLADMIATAISLPFTERQKLIT
                     ITDPQERLKHLNWLLAQELDVLQLEDEIQTRVQSEVDRSQREFYLREQMKAIQTELGD
                     GDPWMREIEELRNRINQSHLPDAARTVALKELERLAQMPQMAPEVGMLRSYIDWLLEL
                     PWDTETEDNLDVRHAAGILDQYHYGLGKAKDRILEFIAVRSLKPRRTRQPILCFVGPP
                     GTGKTSLGRSIAEALGRKFVRLSLGGVRDEAEIRGHRRTYIGALPGRILQTMRRAGST
                     NPLFMLDEIDKLGADFRGDPAAALLEVLDPEQNAAFSDHYLEVDYDLSRVMFITTANS
                     LGSIPPALLDRMEVLDFPGYIEEEKLEIARRFLIPRQVEESGLETKEVTFPDQTVRRL
                     IREYTYEAGVRNLEREIGRMCRKVARQKAEGKPQISRLTPATVERFLGPPQFFVTEAE
                     RQDEVGVATALAWTESGGDIMPVEILILDGKGSLQITGQIGEVMQESAQAALSYLKSR
                     AAQLQIDPSIFEKVDIHIHIPEGAIPKDGPSAGITIAAALISAFTERPVYRHVGMTGE
                     ITLRGRVLPVGGVREKILAAHRAGLKVVLLPHLNLKDLVDVPKKVMTDLKIVPLHHMD
                     EVLEMALYPPTEKAPRPARRKRAPEAETPSENE"
     gene            complement(8920..10305)
                     /locus_tag="PKL11_06660"
     CDS             complement(8920..10305)
                     /locus_tag="PKL11_06660"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNZ13321.1"
                     /translation="MNSLDDAYQQSLDYLYSFVDYSLTHNLRFSPEKFDLARMHDLMA
                     RLGNPHQKYPVIHVAGTKGKGSTAAMTAAALQAAGYRVGFYTSPHLEDFNERIQVNGQ
                     PISRADLVMLVDQLRPHAAAIERITTFELTTALGFMYFAHAHVDVAVVEVGLGGRLDA
                     TNVVTPLVSVITSLSYDHMAVLGNTLAQIAAEKAGIIKPGRPIVLAPQKEEARLVVEQ
                     IASERSAPLTQVGKDLFFAPWSHSLDGQTLLIWSPVEQSLVDEYIESDGHSNWQPIRL
                     TIPLLGHHQIENAATAYTALQAANAAGLSISDAAIQQGLLQTHWPGRFEILRRDPPLV
                     IDSAHNRDSALKLRLAVEDYFPGKPVVLLFGASEDKDIAGMFAELMPRVWQVIATQSV
                     HPRAMPPEQLVALAHQFGVRARVVLPLEAALEQALQTAGPDALVLGTGSIFVAAAIRS
                     AWQKLNTTGRG"
BASE COUNT         2233 a         2672 c         3148 g         2112 t
ORIGIN      
        1 cggtagcctt aggccgactt cgccatcgag cggatgcact tggcgcacag caccttgcga
       61 accttacgac cattcacgaa aaccgacaca cgctgcaggt tggggtgaaa ggtacgattg
      121 gttgcccgct gggaaaagct gcggttatga ccgaaggttg tcgtcttacc acaattatcg
      181 cacttagcca tcttgaaaat cctttccgaa ccagaattgc atcaaaccgc cggcctctgt
      241 ctttgaggca gcaaggctgg attttaccac aaggcgcatc tttttacaag ttgcgtgtta
      301 aattagagaa ggtaaaaaac accatgagcg aaccgacaaa tccatttggc acaattttca
      361 tctccaagcg agcgattact accattgcag cccaatctgc gttagaatcg tacggcgttg
      421 ttggcctggc agccaagaac ctggctgaca gcctggccca ggcgctggta aaagatccgg
      481 cgctgggcgt taccgtccac tatgacggcg ggcaagtgca gattgacctg tatctgatca
      541 ttgaatacgg gctgcgcgtc accaccgtgg caaacagtgt gattgagagc gtgcgctacc
      601 acatcgaaaa ggccaccggg ctggtggtta agcaagtgaa tgttcacgtc cgcgggctgc
      661 gcatcagcaa ccccgattaa cgtcattatc cggtattgga gtcctatgag tactgccgaa
      721 ttttattctg ttgaccgcca gcagctggcg caagcgacca cgctggatgg tcagggcttg
      781 aagacgctgg tagaagccgc gctgacttgg cttaagacca accagcaaac ggtgaacgcg
      841 ctgaatgttt ttccggtgcc tgatggggat actggcacca atatgctgtt gactctgcag
      901 gctgcctata acgaagtggc caactctggc gagcgcaatg tgggcaaaat ggcccatgcg
      961 attgcccagg gggcgctgat gggcgcccgg ggaaattctg gcgtaattct ttcgcagatc
     1021 tggcgtggtt ttgcgcgcgg actggacagc ctggatgtaa tggatgcggc gattatggtg
     1081 cgggccttga ttgaaagccg caacacggct tacaaggggg ttgtgcggcc tgtagaaggc
     1141 accattttga ccgtcatcaa ggatgtctcc atcgctgcag aacaggccct ggctgaaacc
     1201 caggatttga atctgctgct tgaacagatt gtccaggctg cggatgtgtc ggtcaaacat
     1261 accccggaac tgctgccgat tttgaagcaa gcgggggtgg tcgattccgg gggcaaggga
     1321 ctattcttca tttttgaggg aatgctgcgc tacttaaagg atcaaccgct ggaaactgca
     1381 acgacgaccg taatgccatt ggccgccatg caattggagg aaacaatgga agccgtggaa
     1441 gccggacagg attttgaagt ggtggttgat tttcggccca atgagccgct gaatttggaa
     1501 agcttttacg ctgaactttc ggagatggga acgtccattc aggttggcga aggcgatggc
     1561 ttctaccgca tgcatatcca cgtgcctacc gaaaaccgct acctgcccat tgattacaca
     1621 atgaagattg gggcagtgac taannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnaaaaata ttgtactggc agcccaggcg gcagccagta tgacggtcaa gaaagtggca
     1801 gtgatcccca gcaaaagcgt gccgcaaggg ctctcggcta tgctgcggct aaacccggaa
     1861 ggcgatttcg acgaagtggt ggcagagatg aacgaggcca ttacggaggt tgaatccggt
     1921 gagattacgg ttgccacccg ttcgattgaa atcaatgggg tcagtgtaaa agaaggggaa
     1981 gtgattgcgc tgctcaacgg gcagttgatt gccgcccagc ctgatctgga gaaggcctgc
     2041 ctggccctgc tggaaaaggc caacacagcc gatcgtgagc ggattacctt cttctatggg
     2101 cagaatattg gccgagctga ggttaaccgg attgtggata tcatccgcac agcatatccc
     2161 acccatgaga ttgaactgca cgaaggcggg cagccccact atcaatttat cctgtccatt
     2221 gaatagctat gtcatcgatc tgcattctta cggatagttc ggcgcagttc ccacagccta
     2281 catttccggg ccagaatatg gtaaggatga tttcgctggg ggttgagctg gctggaaaaa
     2341 cctataccga cagcaaagaa atccggctga atacgctgcc ggctgcggca acgccggcgc
     2401 tgcacccacg cttgctgccg ccatccgtgg aacagtttca gcaaatgttt atggatttga
     2461 gcagccagca cgatgccgtg ctggttattt tgctatctga gcagctgcat tcaacctgct
     2521 ccaatgcaat gcaagcggcc tctgcactgc aaagctgcac caacgtccag gtgattaact
     2581 cacaaaccac gtcagtgggc ctggggctgc tggtgcaaac cgccgccgag gccattgcgg
     2641 ggggagccag cccggcgcat gccgagcgcc tggtgcgcag tatgatcccg cacgtctata
     2701 cgatcatctg ttcgcaggga ttatcttatt tgcattactc cggatttctc gatcatgccc
     2761 aggccagaat tggtgaaatg ctggggctgc tgccgctatt tacgctggaa gaggggaaat
     2821 taaccccgct ggagaaagtg cgcagcctga ggcaggtatt ggatttcttc caggaatttt
     2881 taagcgagtt tgatcatctg cggcacattg ccctgctgca aggcacacta gggtttggtt
     2941 ctgaagcccg gctgctgcgg cagtttgccc aggagaatta cgccaaaacg ccctacagcg
     3001 aacacacctt caatacgccg ctggcgagcc tgctggggcc gcgcagcctg tgtttgattg
     3061 cggcagaaga acgctaaagc tgactgtttt acggtacaat tggaccatgt tatctgccat
     3121 agaaaaatta ttaaaattca tgcgtctgga agttgatcgc gggtacgaca accgcgctgt
     3181 cgttggtgga ctgaacaaag tgctgccctg gtgggaacca gaagcccgcg cttcggggat
     3241 ggacgccggt ttactggaac gcgtggtgga gcagttgaat gcctacccgg gttacagcct
     3301 ggaagaccgg gctgcctgcc tgaatgacct gttgaatgag ttggaggcgg cccttccggc
     3361 cgaaaccgaa cgccggactg ccaccccgcc gcaccctcgc ccggtggatt ctgacgcaca
     3421 atctgcgccg tcagagcgca gtcggcgccc ggcggttgaa cgcgaaccca gcgcccccat
     3481 tgggctggat gcgccggtaa cggtactgcc cggagtgggg cccaagtatg ctcagacgat
     3541 gaagcaattg gggctgaata ccctggctga tttattgtat tatttccccc gccggtatga
     3601 tgattacagt caattgaaga cgatcaaccg gttggaatat ggcgaagaac tgaccatcat
     3661 cgcgacggtg caaagcgtgg caacccgacc actgcgcggg gggcaatcct cgatgattga
     3721 ggcggttgtc agtgatggca caggctccat ccgcattacc tggtttaatc agccctggat
     3781 agccaaccgg cttaatcgca atgatcaaat tgccatttcg ggcaaggtag atatgtacct
     3841 ggggcggctg gtgatgaaca gccccgattg ggaaccgatt gaacagaaca gcctgaacac
     3901 caatcggatt gtgccggtgt acccgctgac agcgcatgtg acgcaaaaat ggctgcggcg
     3961 cttaatgtat gacacggtta cctactgggc cgcgcggatc acggattatc tgcctgagga
     4021 tattcgccgg gcagtggatt tgccacccct gggccccgcc ctggtgcagg cgcatttccc
     4081 cgattcgatg gaacggctgc gagctgcccg cgcccggctg gcctttgatg aaattttgct
     4141 gctgcaactg ggtgtgctgc agcaaaaaag caactggcaa tcggttgcgg cacgcgtctt
     4201 tgagacgccg gatgcgtggc tggacgcgca ggttcagcgg ctgccctacc cattaaccgg
     4261 cgctcagcag cgcgccctgg cgcatgtgcg cgccgacttg agttctggac gcccgatgaa
     4321 ccgcctgctg cagggtgatg tgggttcggg caagacggtg attgcagcat tgggcgcagc
     4381 catggtcacc cgggccggcg cacaggcagc catcatggcg ccgaccagta ttctggcaga
     4441 acagcactac cgcaacctga cccggatgct gaccgagccg ggcgataacg acaacgcggc
     4501 cctcaagccg gaggaaattc gactgctggt gggcgatacc cccgaagctg aaaaggccga
     4561 aatccgcacc gggttagaag aaggcaatat caagctgatt attggcacgc atgctttgat
     4621 tgaagcgccg atcagcttta atcaattgga atttgtggtg attgatgaac agcaccgctt
     4681 tggtgtggcg cagcgcgcgg cactgcgtgt gaagggtcaa accccgcacc tgctggtgat
     4741 gacagcaacc cccatccccc gctcgctggc gctgacgctg tatggcgacc tggacctgac
     4801 ggtgatggac gaaatgcccg ccggacgcca acccgtggaa acgcatgtac tgcatccact
     4861 ggagcgcgag cgcgcctacc agttaattcg cacccaggtg cagcaaggac gacaggcttt
     4921 cattatctac cccttgattg agaagggtga taaagaagaa agcaaggctg cggtggatga
     4981 acacgagcgg ctgcaaagcg tcatcttccc acggctaaag ctggggttgc tgcatggcag
     5041 gatgcgcccc gacgaaaaag aacaggtgat ggcgcgcttc cgagacgggg aatttcagat
     5101 ccttgtttcg accacggtgg tggaagttgg cgtggatgtg cccaatgcga ccgtcatgtt
     5161 gattgaaggg gcaaaccgct ttggcctggc gcagctgcat caattgcgcg ggcgggtggg
     5221 ccgcggcgga gaccaggctt actgcctgtt gatccctgag aatgaaaacg cgattgagaa
     5281 tgagcggctg gcggtgatgg ccgagaccaa tgatggtttt gtgctggctg agcgcgacct
     5341 ggatcagcgc ggaccgggcg actttttggg cacccggcag gctggctttg cccagttacg
     5401 tatggcctcg ctgaccgatg tgcgcttgat tgaaaaggcc cgcgaacagg ctatgaaaat
     5461 taatgaccgc gacccggatt tatctgcccc ggaaaacgcc gccctgcgcc gaactttgca
     5521 gcggttttgg aaccccggaa aaggcgatat cagctaaaat agnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nngatattga ggtgatggcg ctgatcacca atgaggaaca
     5701 cacttttctt tcctccacca ccgtgcgaga aatcgcctcg ctggggggag atgtgagcag
     5761 catggtgccg ccctttgtag caaaagccct gtatcagcgt tttggtgaga ttggcgaaaa
     5821 tcaccagcat atgacatccc tgcgagattg agcaagctgg cccaatcctt gcaacgattt
     5881 gtggtgtgag atcacccgga ggaagaaatg gacattctgc atctcgttga ccgcctggaa
     5941 gaagttttca acgaaggccg ctcaatctgg tttacccgcc aggtagccgt ggacgaagac
     6001 cggatgctgg acttaattga ccagatgcgg atttcgattc ctgaagaaat caagaaggca
     6061 cagcaaatta ccgcccagcg agaccggatg ctggctcagg cccaggaaga agccaaccgg
     6121 acggttcagc aggcccgcga acgcgcccaa cagatgattg agcgcgatga tgtaaccaaa
     6181 gaagccaggg tgcaggctga gcagattttg gcggctgccc gggatgaagc tgaaattacg
     6241 cggcgagacg ccgatgctta tgtgttgaaa tcgctgacgg atatggaaat ggaattagag
     6301 cgcctgctga cgcaggtgcg caacggcatc cataccatca aagaaactca aaacccgacc
     6361 aacgaagcct gaaacggctt attcgttttc tgaaggcgtc tcggcttcgg gagcgcgctt
     6421 gcggcgcgcc gggcgaggcg ccttttctgt tggcgggtaa agcgccattt ccagaacttc
     6481 gtccatgtgg tgcaggggga cgattttcaa atctgtcatg acctttttgg ggacatcgac
     6541 caggtctttc aggttgaggt gaggcagcaa aacgaccttc aagccagcgc ggtgggcggc
     6601 caggatcttc tcgcgcacgc cgccaaccgg cagcacccgc ccgcgcaggg taatctcacc
     6661 tgtcatgccg acatgccggt aaaccgggcg ttcggtgaag gcagaaatca gggcggcggc
     6721 aatggtgatg cccgcgctgg ggccgtcttt ggggatggcg ccctcaggga tgtgaatgtg
     6781 gatgtcgacc ttctcaaaga tggacggatc gatttgcagt tgagctgcac gggacttgag
     6841 ataagacagc gcagcctggg cggattcttg catcacttcg ccaatctggc cggtaatttg
     6901 caaactgccc ttgccatcca ggatcagaat ttcaacgggc atgatgtcgc cgccgctttc
     6961 cgtccaggcg agggctgtgg cgacgcccac ttcatcctgg cgctcggctt cggtcacgaa
     7021 gaactgcggc gggccgagga agcgttccac cgtggcgggg gtcagacgcg aaatttgcgg
     7081 cttgccttcg gccttctggc gggccacttt gcggcacatg cggccaattt cccgctccag
     7141 gttgcggaca ccggcttcgt aggtatattc acgaatgagg cggcggacgg tctggtcggg
     7201 gaaggtgact tctttagtct ccaggccgct ttcttccacc tggcggggga tgaggaagcg
     7261 gcgggcaatt tccagctttt cttcttcgat gtagccgggg aagtccagca cttccatgcg
     7321 gtcgagaagg gccggcggta tgctgcccag cgagttggcg gtggtgatga acatgacgcg
     7381 ggaaaggtcg taatcgacct cgagataatg atcggagaag gcggcgttct gctcggggtc
     7441 cagcacctcg aggagtgcgg cggcagggtc gccgcgaaaa tcggcgccaa gtttatcaat
     7501 ttcgtccagc atgaagagcg gattggttga acccgcccgc cgcatggttt gcagaatacg
     7561 cccggggagc gcgccgatgt aggtgcggcg gtggccgcgg atttcagctt catcacggac
     7621 gccgcccagg gaaaggcgga cgaacttgcg ccccagagct tcggcgattg agcgccccag
     7681 cgaggtcttg ccagtgccgg gcgggccgac aaaacagagg atgggctggc gcgtgcggcg
     7741 gggtttgagg ctgcggacgg caataaattc cagaatgcgg tctttggctt tgccgaggcc
     7801 gtagtggtat tgatccagga tgccagccgc gtggcggaca tccaggttgt cttcggtttc
     7861 ggtgtcccag ggcagttcca gtagccaatc gatgtaggag cgcagcatgc ccacctctgg
     7921 cgccatctgg ggcatttgag ccaggcgttc aagctccttg agggcaacgg tgcgggcagc
     7981 atcgggcagg tgcgattggt tgatccgatt gcgcagctct tcaatttccc gcatccacgg
     8041 atcgccatct cccagttcgg tctggatggc tttcatctgc tcgcgaagat aaaactctcg
     8101 ctggctgcga tccacttcgc tttgaacgcg ggtctggatt tcgtcttcaa gctgcaagac
     8161 atccagttct tgagccagga gccagttgag gtgcttgagg cgttcctggg gatcggtgat
     8221 ggtgatgagt ttttggcgct cggtgaacgg cagcgagatg gcagtggcaa tcatatcggc
     8281 cagccagccg ggttcttgaa tgttcaacga gaagagatgt gcttcttctg gcagactgcg
     8341 atccagttgg acacagcgct caaacagatc gcgcgaggtg cgcatgagag cttcggtctg
     8401 gcgatctaca tgggtggatt caacgatcac acgggcgcgg acacggaaat aaggctcggt
     8461 ttgcgtaaat tccagcactt ccaggcggcg gcggccctgg atcagggcgg agttgttgtc
     8521 atcgggcatg gacagcagcc gaccaaccgc cacctcaacg ccaataggga ggaaatcttt
     8581 ttgggtaggc tcggcttcag catccgggtc ggagacaatc agggcgatca tggtctcgta
     8641 ggcttcctgg gcatcttcaa tcgccagcag gttgggaccg ggtgggacaa agatgggtga
     8701 gaccatgtgg gggaacacca ccacatcccg cagaatcagg acgccagctt cgatggtgcc
     8761 atcttcattg acggtcatat ctgggacctg atagagttct tcagtccgct ggtgcagtgc
     8821 ggagatatag tcttctgagt cgtcggggcg cgacgcgcgt tgattatctt tcatgcggtt
     8881 tggttccttc aaggtcaatt ataccgaaaa gcggcagctt tagccgcgcc cggtggtgtt
     8941 cagtttttgc caggcagagc ggatggctgc tgcgacaaag atgctgcccg tgcccagtac
     9001 cagggcatcg ggcccggcgg tttgcagggc ctgctccagg gcggcttcca gtggaagcac
     9061 gacccgggca cggaccccaa attgatgcgc cagggcgaca agttgttcgg gcggcatggc
     9121 gcgcgggtga actgactggg tggcaataac ctgccaaacc cgaggcatta attcggcaaa
     9181 catgccggca atatctttat cttcagatgc gccaaagagg aggactaccg gcttgccggg
     9241 gaaataatct tccacggcca ggcgcagctt gagggcggaa tcacggttgt gcgccgagtc
     9301 gatcaccagc ggaggatcac ggcgcagaat ttcgaaccgg ccaggccagt gggtttgcag
     9361 caaaccctgc tggatggctg catctgagat ggacagacca gcggcattgg cggcctgcag
     9421 cgccgtgtag gcggtagcgg cattttcaat ctgatgatgg cccagcaggg gaatggtcag
     9481 gcggatgggc tgccagttgg agtgtccatc ggattcaatg tactcatcaa caagcgattg
     9541 ctcgaccgga gaccagatga gcagagtttg cccgtccagg gagtgcgacc agggggcaaa
     9601 aaacaggtct ttgccgacct gagtgagggg agcggagcgc tcagaggcaa tctgctcaac
     9661 caccaaccgg gcttcttctt tctgcggggc cagcacgatg ggccgcccgg gtttgatgat
     9721 gcccgctttt tcggcagcaa tctgggcaag ggtgttgcca agcacggcca tatgatcgta
     9781 ggagagcgag gtgatgacag agaccagcgg ggtgacgacg ttggtggcat ccagacggcc
     9841 gcccaggccg acctcaacca cagccacatc cacgtgggcg tgggcaaaat acataaatcc
     9901 gagggcggtg gtcagctcaa aggtagtgat gcgttcaatt gcggcggcgt gagggcggag
     9961 ttgatccacc agcatgacca gatcggcccg cgagatgggc tgcccgttga cctgaatgcg
    10021 ctcgttgaaa tcctccagat ggggggaggt atagaagccc acccggtagc cggcagcctg
    10081 caaggcggcg gcagtcatgg cggcggtaga gcccttgcct tttgtaccag caacatggat
    10141 gaccgggtat ttctggtggg ggttacccag gcgcgccatc agatcgtgca tgcgcgccag
    10201 gtcaaacttc tcagggctga agcgcaggtt gtgcgtgaga ctgtaatcta caaaactgta
    10261 gaggtaatcc agcgactgct ggtaagcatc atctaatgag ttcattgatt cttccaatcg
    10321 aagcgtgatt gtactaaagc gcgcggcttt gtgcaaggtc gaatc
//
LOCUS       DAOPVS010000064        10106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6710_length_10106_cov_55.945876, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000064 DAOPVS010000000
VERSION     DAOPVS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6710_length_10106_cov_55.945876"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..506
                     /locus_tag="PKL11_06665"
     CDS             39..506
                     /locus_tag="PKL11_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ13322.1"
                     /translation="MPTHYSGTTEETLALNTYIKLTRAVNTVEARVAHQGAAGDLTPS
                     QFGVLEALMHLGPLCPGELSAKLLKSTGNITLVLDNLEKRGLIQRERTVEDRRMIKVS
                     LTEAGKALIERIFPDQVKAITTAFCSLSAEEQSLLGELCRKLGKTESVNTLPE"
     gene            546..1070
                     /locus_tag="PKL11_06670"
     CDS             546..1070
                     /locus_tag="PKL11_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNZ13323.1"
                     /translation="MSWQIDTAHSQIQFTVRHLMISKVRGSFEKFSGTVALDESAPAN
                     TTVDVQIETGSVNTREPQRDGHLRSPDFFNSEAFPLMTFKSKRVDVVDGHHAKLIGDL
                     TIRDVTREVTLDVEYLGQAKSPWGTTSAGFTATTKINRKDWNLTWNQALETGGVLVGE
                     EVEISIELELVKQA"
     gene            1225..1965
                     /locus_tag="PKL11_06675"
     CDS             1225..1965
                     /locus_tag="PKL11_06675"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HNZ13324.1"
                     /translation="MDDLLTTRQVQDRLKVDRITVYRMLQDGRLKGVKIGQQWRFATR
                     EVERLLGGAPAEETVGETAAMRAFPTHCVQTIQNLFSDISQTGALVIDPQGEPLTEPS
                     ALPAFCQLMLNNPRGREACQKTWQAMAQVGGSGGTFTCHAGLHYCAAPIQEDGEQVGW
                     FLAGQMHLQAPDAEEQAALITHLAAAYGLDAAELAKAARQIPVAAGSDGAPCLSWTDR
                     ARQAVEAILKERAGLVERLQKIAEITQI"
     gene            2000..3232
                     /locus_tag="PKL11_06680"
     CDS             2000..3232
                     /locus_tag="PKL11_06680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNZ13325.1"
                     /translation="MKTFLILGGGTAGTILAHNMVKKLDTEQWKIIVVDRDKTHYYQP
                     GFLFIPFGIYKPEDTIQPKQKYLPRKVEFVLSDIELIEPDKNRVTLTEGKRVINYDYL
                     AIATGTDIRPSEVEGLLGAGWRKNVFDFYTFEGASALGEFMPKFEGGRLVIHITEMPI
                     KCPVAPLEFAFLADWYFTKRGIRNKVEIVYATPLPGAFTKPRAAAVLGDMFKKKGIHL
                     EPDFNVSEIDSGKNTISSYDGRELPYDLLVTVPTNMGAEVIGRSGMGDDLDFVPTNKH
                     TLQSNKWPNVFVLGDAANIPASKAGAVIHFQMEAAVENILHHMAGKEMHAEFDGHAIC
                     FIESGYNKAALIDFSYDQEPLPGKYPFPGIGPMTLLGESVLNHWGKLGFRYMYWDLMM
                     RGINVPMPAKFSMTGKIK"
     gene            3277..3582
                     /locus_tag="PKL11_06685"
     CDS             3277..3582
                     /locus_tag="PKL11_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HNZ13326.1"
                     /translation="MSDILSTVAFDADGFMVDANAWTPEIAAAIAAREGITLTDRHMV
                     VINYARATWKATGDAPTLRNITKNTDVGTKELYELFPGGPGKLAAKVAGLKKPTGCI"
     gene            3615..4091
                     /locus_tag="PKL11_06690"
     CDS             3615..4091
                     /locus_tag="PKL11_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNZ13327.1"
                     /translation="MADAPRKLSIIASKGSLDMAYPPLILANAARMSGVEVHMFFTFW
                     GLDIITKKKMDHLNVAMVGNPNMHPMMHIPTWIGAIPGMSALGTWMMNKEIAKLDFPP
                     VGEFVQMLQDAGAHIYGCKMSMDMMKLTDADLVDGAKTLGAMEFIELSEGGQIIFV"
     gene            complement(4163..5425)
                     /locus_tag="PKL11_06695"
     CDS             complement(4163..5425)
                     /locus_tag="PKL11_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13328.1"
                     /translation="MESNWKRTFFLIWTGQAFSLLGSSLVQFALVWWLTQKTGSATVL
                     AAATFVALLPSVFLGPFVGALVDRWNRRIVMIAADGMVALATAALVILFWLDIAQVWH
                     VYVVLFVRSLTGTFHFPAMQASTSLMVPDKHLSRISGLNQALRGILNIAAPPLGALLI
                     GLIPMAAVMAIDIVTAAIAILPLLFVSIPQPQQANPSEAVTPTTVLRDVAAGLKYVKA
                     WPGLLGVIILAMLLNFVIAPSGTLMPLLVTKHLNGDAYLLGFMESAFGAGIIVGGIIL
                     AVWGGFKRRIITSMSGVIALGAGVLFLSAATKESLWLGLAAQGLMGFAIPFANGPLMA
                     IMQSRVAPEMQGRVFTLLESGATAMMPLAMIIAGPVADALGIRTWYVIGGAACILIAL
                     GGLLVRPIMQIESNGHAEPVPQPVPEAI"
     gene            complement(5436..5846)
                     /locus_tag="PKL11_06700"
     CDS             complement(5436..5846)
                     /locus_tag="PKL11_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13329.1"
                     /translation="MGVNREERLKILQMLQEGKISAEQATQLLEALEGARTPVSPMPP
                     VPPEPPLPPRVSGAGRWLRVRVTDSDTNKVRVNLRLPLSVVESGLKMGMRFTPEIQDI
                     DPQELMGILNSGQIGQVVDVYDEKDGEHVEVFIE"
     gene            complement(5871..6275)
                     /locus_tag="PKL11_06705"
     CDS             complement(5871..6275)
                     /locus_tag="PKL11_06705"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNZ13330.1"
                     /translation="MNRLLSKCPVCEGEMFVTRLYCPQCDTGVEGHFSPSANPLSPLT
                     SEQVKFVMTFIRCEGRFNRMEEELGLSYPTLRNRLYDIIRALGFEPGKDETIRLTNEE
                     RMQILDELARGVISAEDAERRLLGKKDESEDK"
     gene            6482..6931
                     /locus_tag="PKL11_06710"
     CDS             6482..6931
                     /locus_tag="PKL11_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13331.1"
                     /translation="MQISEVKQVNDEIVEAFNRLIPQLSTTNLPPTREELEEIVAAGT
                     SHLLIARDDTGRIVGGLTVVIFRIPTGLRTRIEDVVVESSARGQGIGEALSRAGIEMA
                     RSLGAESVDLTSHPSRIAANRLYQRLGFTIRHTNVYRYIFPTHGATS"
     gene            6968..7198
                     /locus_tag="PKL11_06715"
     CDS             6968..7198
                     /locus_tag="PKL11_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNZ13332.1"
                     /translation="MENRRLYRSRADQMIGGVCSGLGSYLTIDPTLVRLIFVLLFLFG
                     GNGLLIYIILWIIVPIEPELPASGGDQPPAVS"
     assembly_gap    7262..7361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7362..8971
                     /gene="murJ"
                     /locus_tag="PKL11_06720"
     CDS             <7362..8971
                     /gene="murJ"
                     /locus_tag="PKL11_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNZ13333.1"
                     /translation="IPAIIRGMQSNPASRQIARAAGMVAAAMIISQLIGLVRGILIYR
                     TFGTTADLDSFNAANRVTEVLFNLMAGGALGSAFIPTFTGLLAKDNRQRAWKLAAAIA
                     NLLILVLSGVTLLAAIFAPQIVRHGLFILAPDLSTGQELLTIRLMRIMLPSVVIFGLS
                     GLAMGILNAHQIFWMPALAPAMYSIGMILGVAIFPASWGIDRLAWGALLGSGLHLLVQ
                     MPGLIRLRGRYSVTLGRDIQEVGEVMRLMGPRILGVAVVQINFIVNTIIALSLPEGSV
                     SAVTLAFALMLMPQAAIAQSLAIAAMPTFSAQVALGKLDEMRAAFAATLRGALLLAIP
                     AAVGVILLRYPIIRVLYERGEFTAHSTDLVTWALLWYSIGLVGHSLVEISSRAFYALH
                     NTKTPVLVGVAAMSLNLVFSLTLPAGFAQLGWMPHGGLALANTLATALEAAALLVLLR
                     RQMKGLNGRAIGQVTVQAGLAATAMALGLALWLMATRNIPNWIQAVGGVAVGSGLYGL
                     LLWGLRVHEVSRVMAAVRRRLPGRFQS"
     gene            9000..9746
                     /locus_tag="PKL11_06725"
     CDS             9000..9746
                     /locus_tag="PKL11_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNZ13334.1"
                     /translation="MTKNSGHDFMQRTRYTEMGPSAQSQGLPQPALETDYPLQAKLIS
                     LPDPAELTMPGVDLRRAIEERVSIRQYSQEPLTIEELTYLLWTTQGVKAVTARPVTLR
                     TVPSAGARHAFETYLLINRVSGIEPGLYRYLAIENALLPITMGPEIATQLTEACLKQQ
                     MINTSAVTFFWTAAVERMFWRYGERGYRYLHLDAGHVCQNLYLAAWEINCGTCAIAAF
                     DDDMLNAALGLDGETTFTVYLAAVGRKGKC"
     gene            complement(9845..10093)
                     /locus_tag="PKL11_06730"
     CDS             complement(9845..10093)
                     /locus_tag="PKL11_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNZ13335.1"
                     /translation="MNLFATIVVGLIAGFLASKIMKTNTSIWVELILGVVGSIVGGLI
                     GSLFGLDLMTGINLTSILLALAGAIIVIVIYRFIKRRK"
BASE COUNT         2295 a         2578 c         3003 g         2130 t
ORIGIN      
        1 tatctctaca tcaagatatt tgatatagag atataaacat gccgacacat tattctggaa
       61 caactgaaga aacactggcg ctgaacactt atatcaaatt gacccgggcg gtcaatacgg
      121 ttgaggcaag agtggcacac cagggagccg cgggcgacct gacgccaagt cagtttggcg
      181 ttttggaggc attgatgcat ctgggaccgc tctgccccgg cgaactgagc gccaaactat
      241 tgaagagcac tggcaatatc accctggtgc tggataacct agagaaacgc ggcttgattc
      301 aacgagaacg caccgtcgaa gaccggcgga tgatcaaggt ttcgctcacg gaagcaggca
      361 aggcgctgat tgagcgcatt tttccagacc aggtgaaggc aattacaacg gcattctgtt
      421 cgctttcagc cgaggagcaa agcctgctcg gggaactgtg ccgaaaactg ggtaaaacgg
      481 agagcgtgaa tacgctccct gaataagaca ttttatttgt agaactactt tctaaggaga
      541 aaaacatgtc ctggcaaatt gatacagctc actctcaaat tcaattcact gtgcgccatt
      601 tgatgatttc aaaggtgcgc ggcagctttg agaaatttag tggaacggtg gctctggatg
      661 aatcggcacc ggcaaacaca acagtggatg tgcaaatcga aaccggcagt gtgaacaccc
      721 gtgaaccgca gcgcgatggg cacctgcgct cgccagattt cttcaacagc gaagcttttc
      781 cgctgatgac gttcaagagc aagcgggtgg atgtggtaga tggtcaccat gctaaactga
      841 ttggcgacct gacgatccgg gatgtgaccc gggaggttac actggatgtg gaatacctgg
      901 ggcaggccaa gagtccgtgg ggaaccacca gcgccggctt tacggcgaca acgaaaatca
      961 accgcaaaga ttggaacctg acctggaatc aggcactgga aaccggcggc gtgctggtgg
     1021 gagaagaggt tgagatttcg attgagttgg aactggtgaa gcaggcgtag tctctttcgt
     1081 tttacaagag gataaacaac cgaggctttt gcctcggttg ttgcaatatg acatatatca
     1141 cgtgacaaat atcactattc gctctacgct attaggtgta ttatatcaac gtataacatg
     1201 ctataacaag ttataactat atttatggac gacttactca ccacccgcca agtacaggac
     1261 cgcctcaaag tagaccggat tacggtctat cgtatgctgc aagatggacg cctcaagggc
     1321 gtcaagattg gacagcagtg gcggtttgct acgcgggaag tagagcggct gctgggcggc
     1381 gcaccggcag aggaaaccgt gggggaaacg gctgcgatgc gggcgttccc aacccactgc
     1441 gttcagacaa ttcaaaacct tttcagtgat atcagccaga cgggggcgct ggtgattgat
     1501 ccgcagggtg aaccgctgac cgagcccagc gcgctgccgg cgttctgcca gttgatgctc
     1561 aacaatcccc gagggcgcga ggcctgtcaa aaaacatggc aggcgatggc gcaggtgggc
     1621 ggcagcggcg gtacattcac atgccatgcg gggctgcatt actgtgcggc gcccattcaa
     1681 gaagatggcg aacaggtggg ctggtttttg gcgggtcaaa tgcacctgca agcgccagat
     1741 gccgaggaac aggcggcgct gatcacccat ctggcggcgg catatggtct ggacgccgca
     1801 gaactggcga aggctgcccg gcagatcccg gtggcagccg gcagtgatgg ggcgccctgc
     1861 ctgagctgga ccgaccgcgc ccggcaagcc gttgaggcca ttttgaagga acgcgccggg
     1921 ctggttgaac ggctgcagaa gattgctgaa atcactcaga tttgatttta ttgctagact
     1981 ggaaataagg agcaaacaca tgaagacatt cctcattttg ggtggcggca cggctggaac
     2041 cattctggct cataacatgg tcaagaagct ggatacggaa cagtggaaga tcattgtggt
     2101 ggaccgcgac aagacacatt attatcaacc cgggttcctg ttcatcccct ttgggattta
     2161 taaacccgag gataccattc agcccaaaca gaaatacctg ccgcgcaagg tggaatttgt
     2221 gctctcggat attgaactga ttgagccaga caagaaccgg gtcacgctga ctgagggcaa
     2281 gcgggtgatc aactatgatt acctggcgat tgctacgggt acggacatcc gcccgagtga
     2341 agttgaaggg ctgctgggcg cgggctggcg gaagaatgtc ttcgattttt acacctttga
     2401 aggcgcttcg gcgctaggcg agttcatgcc taaatttgag ggcgggcggc tggtgattca
     2461 catcacagaa atgccaatta aatgcccggt ggcgccgctg gaattcgcct tcctggcgga
     2521 ctggtacttc accaagcgcg ggatccgcaa caaggtggag attgtgtatg ccacccctct
     2581 gccgggcgcc tttaccaagc cgcgggcggc agcggtgctg ggcgatatgt tcaagaaaaa
     2641 gggcattcac ctggagcctg atttcaatgt cagtgaaatt gactcgggca agaacactat
     2701 cagttcttat gacgggcgcg aactgccata tgacctgctg gtgactgtgc cgaccaatat
     2761 gggcgccgag gtgatcgggc gttcgggcat gggggatgac ctggattttg tgccgaccaa
     2821 caagcacacc ctgcaatcca acaaatggcc caatgtcttt gtgctgggcg atgcggcgaa
     2881 tatcccggct tctaaggcgg gcgcggtgat tcacttccag atggaagcgg cggtagagaa
     2941 catcctgcat catatggccg gcaaggaaat gcacgccgaa tttgatggcc atgcaatttg
     3001 cttcatcgag agcggataca acaaggccgc actgattgac ttcagctacg accaagaacc
     3061 gctgccgggt aaatatccct tcccgggtat tggcccgatg acgctgctgg gcgaatcggt
     3121 gcttaatcac tggggcaaac tgggcttccg gtacatgtac tgggacctga tgatgcgggg
     3181 cattaacgtg cccatgcctg ccaagttcag catgacgggc aaaatcaaat aacccatctg
     3241 atctcattca accaatcaca aaaaggagat tctgaaatgt ctgacattct ttcgactgtt
     3301 gcttttgatg ccgatggctt catggtggat gcaaacgcct ggacgcccga aattgctgcg
     3361 gcgattgccg cccgcgaagg cattactctg accgaccgcc acatggtggt catcaactat
     3421 gcccgggcga cctggaaagc cactggcgat gcccccacgc tgcgcaacat caccaaaaac
     3481 accgacgtcg gcaccaaaga actgtacgaa ctgttccccg gcggaccggg caagctggcc
     3541 gccaaagttg ccgggttgaa gaaacccacc ggctgcatct aatccatctg atattgattt
     3601 gaggagaaac aaaaatggct gacgctccgc gcaaactgtc aattattgct tcgaagggct
     3661 cgctggatat ggcttacccg cccctgatcc tggcgaatgc ggcccgcatg tctggtgttg
     3721 aggtgcatat gttcttcacc ttctgggggc tggacatcat caccaagaaa aagatggatc
     3781 atctgaatgt ggccatggtg ggcaacccca acatgcatcc gatgatgcac atcccgacct
     3841 ggatcggtgc gattccgggc atgtcggcgc tgggcacctg gatgatgaac aaagagatcg
     3901 ccaagctgga cttcccgcct gtgggtgaat ttgtgcaaat gctgcaagac gcgggcgcac
     3961 atatctatgg ctgcaagatg tcgatggata tgatgaagct gaccgacgcc gacctggtgg
     4021 atggcgccaa gacgctgggc gcgatggagt tcattgagct ttcagagggc gggcagatta
     4081 tcttcgtata atctctccac acacttaaaa gcaaacttgc ggcggaccat gtggttcgcc
     4141 gcaagttttt tataagtcca gatcagatgg cttcgggaac cggttgaggg actggttcgg
     4201 catggccgtt agattcaatt tgcatgatcg ggcgaaccag taaaccaccc agagcaatga
     4261 ggatgcaggc tgcgccgcca atgacgtacc aggtgcggat gcccagggca tcggcaaccg
     4321 ggccagcaat gatcatggcc aggggcatca tggcggttgc gccgctttca agcagtgtaa
     4381 agacgcgccc ctgcatttcg ggggcgaccc tggactgcat gatcgccatc aacgggccat
     4441 tggcaaaggg aatggcaaag cccatgaggc cttgagccgc caggcccagc cagaggctct
     4501 cttttgtggc cgcagacagg aataaaacgc ctgcgccaag ggcgatgacg ccgctcatgg
     4561 aggtgatgat gcgccgcttg aacccgcccc aaaccgccag aatgattccg ccaacgatga
     4621 tgccggcgcc aaaggctgat tccatgaagc ccagcaggta ggcatcgcca ttcaggtgtt
     4681 tggtgaccag caggggcatc agcgtgccag agggggcaat gacgaagttc aagagcatgg
     4741 cgaggatgat gacacccagc agaccgggcc aggctttgac atacttcaaa ccggcagcca
     4801 catctcgaag cacggtggtg ggggtgactg cctcggaagg attggcctgt tggggctgag
     4861 gaatggagac aaacagcagc ggcaggatgg caatggcggc ggtgacaata tcgatcgcca
     4921 tgaccgccgc catcggaatc aggccaatca aaagcgctcc gaggggcggg gcagcgatgt
     4981 tgaggatgcc acggagcgcc tggttgaggc cggagatacg agacaggtgc ttatcgggca
     5041 ccatcagaga ggtggaggcc tgcatggcgg ggaagtggaa cgtgcccgtg agggaacgga
     5101 caaatagaac tacatagacg tgccagacct gggcaatatc cagccagaaa agaatgacca
     5161 gggcggcggt tgccagggca accatgccat cggcggcaat catcacaata cggcggttcc
     5221 accggtccac cagagcgccg acaaacgggc ccaggaaaac ggagggcagc agggctacaa
     5281 acgtggccgc agccaggacc gtggcggagc cggtcttctg ggtgagccac caaaccagcg
     5341 caaactggac taaagagctg cccagcagcg agaaggcctg accggtccag atcagaaaga
     5401 aagttcgctt ccagttcgat tccacttgag gggcctcact cgatgaagac ttcgacgtgt
     5461 tcgccgtctt tttcgtcata gacatcgaca acctggccaa tttgaccgct gttgaggatg
     5521 cccatgagct cctggggatc aatatcctgg atttcggggg tgaagcgcat gcccatttta
     5581 agacccgatt ccaccacgct gaggggcagc cgtaaattga cgcgcacctt gttggtatcg
     5641 gaatcggtga cccggacccg cagccagcga ccggcgccgc tcacgcgagg gggcagtggt
     5701 ggttctgggg ggacaggggg catgggagag accggcgtgc gggccccttc cagagcctcg
     5761 agcaactgcg tggcctgctc ggcagagatt ttgccttctt gaagcatttg caagattttg
     5821 agacgttctt cgcgattgac acccatgatc acacctccta tgcgtgatta ctatttgtct
     5881 tcggattcat cttttttgcc gagcaagcgg cgctcggcat cttcggcgct gatgacgccg
     5941 cgggccagtt catccaaaat ttgcatccgc tcttcgttgg tcaggcggat ggtttcatcc
     6001 ttgccgggtt caaagcccag ggcgcggata atatcgtaga ggcggttgcg cagcgtggga
     6061 taagacagcc ccagctcttc ttccatgcgg ttaaagcggc cctcacagcg gatgaaggtc
     6121 atcacaaact tgacctgttc cgaggtcagc ggcgagagtg gattagccga tgggctgaaa
     6181 tggccttcca cgccggtatc acactgagga caatacaggc gggtgacgaa catttcacct
     6241 tcgcagacgg ggcatttgct caataatcgg ttcataaggg ttcctccagg cgtttgaact
     6301 tcaatatcac tttgtcaaac taaatatatt ctagcagata attaaataat gtcaatatgt
     6361 attttgatta ttttgatatt tgctttagat tatttaggac gaattgcgct ttttcaagga
     6421 tatccgaccc gattgagcaa agacaatgcc ggatataatt atggatattc aaggaggaga
     6481 catgcaaata agcgaagtca agcaagtgaa tgacgagatt gtggaagcct tcaaccgatt
     6541 gattccgcaa ctgtccacga ccaacctgcc ccccacccgg gaagagctgg aagagattgt
     6601 ggcggctggg accagccatt tactgatcgc ccgggatgat acggggcgaa ttgtgggtgg
     6661 gctgaccgtg gtgatttttc gcatcccgac agggctgcga acccggattg aggatgtggt
     6721 ggtggaatct tcggcacggg gccagggaat cggcgaggcg ttgagccgcg ccgggatcga
     6781 gatggcgcgc agcctgggag ccgaatcggt ggatctgacg tcgcacccat cgcgtatcgc
     6841 cgccaaccgg ttgtatcaac ggttggggtt caccatccgg cataccaacg tctatcggta
     6901 tatctttccg acacacggtg caacttcctg aggtggattg cgtattttat actgaaggag
     6961 ttacaccatg gaaaaccgaa gattgtatcg ttcgcgtgct gatcaaatga ttggcggtgt
     7021 ttgcagcggc ctgggcagct atttgacgat tgacccgaca ttggtccggc ttatttttgt
     7081 gctgttgttc cttttcggcg ggaacggtct gttgatctac atcattttgt ggattattgt
     7141 gccgattgag ccggaactgc cagcctcggg cggcgaccag cccccggcag tatcctgatc
     7201 ttttgccatt cgccctgccc cgcccggagc gttcctcccc gggcggggct tccataaccg
     7261 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggattcctg ctataatcag
     7381 aggcatgcaa tccaacccgg ccagtcgtca aattgcccgg gccgccggga tggtcgctgc
     7441 ggccatgatt atcagccagt tgatcggtct ggtgcgcggt attttaattt atcgcacttt
     7501 tggcaccacg gctgatttag attcgttcaa tgcggcgaac cgggtcaccg aggtgctgtt
     7561 caatttgatg gccggggggg cgctgggctc ggcatttata cccaccttta cgggactgct
     7621 ggctaaagat aaccgacaac gagcctggaa actggctgcc gcgattgcca atctgctcat
     7681 tctggtgctt tcgggggtaa cactgctggc agcgattttt gcgccgcaga ttgtgcggca
     7741 cggtctgttt attctggcgc cggacctttc gacggggcag gaactgctga ccattcggct
     7801 gatgcgcatc atgctgccca gcgtggtgat ctttggcttg agcgggctgg cgatgggaat
     7861 cctcaacgca caccaaattt tctggatgcc agcactggcg ccagcgatgt attcgatcgg
     7921 tatgattcta ggtgtggcga tattcccggc aagctgggga attgaccggc tggcctgggg
     7981 cgcgctgctg gggtccggac tgcatctact ggtccagatg ccgggtctta tccggctcag
     8041 ggggcgctac agcgtgacgc tgggacgcga tattcaagaa gtcggcgaag tgatgcggtt
     8101 gatggggccc cggattctgg gcgtggcagt tgtacaaatc aattttatag tcaataccat
     8161 catcgcactg agcctgccag agggcagtgt ttcggcggtg acgctggcat ttgcgttgat
     8221 gctgatgccg caagctgcaa ttgcccaatc gctggcgatt gcggccatgc ctacattctc
     8281 ggcgcaggta gcgctgggca aactggacga aatgagagcc gcgtttgcag ccaccctgcg
     8341 cggagcgctg ctgctggcga tcccggcagc agtaggcgtc atcctgctgc gctatcccat
     8401 catccgagtg ctttatgagc gcggtgaatt tacggctcac tcgactgatt tggtgacctg
     8461 ggcgctgctc tggtacagca tcgggctggt gggacactcg ctggtggaaa tttccagccg
     8521 ggcattttat gcactgcata acaccaaaac gccggtgctg gtcggcgtgg cggctatgag
     8581 cctgaatctg gtgttcagcc tgacgctgcc tgccggcttt gcccaactgg gatggatgcc
     8641 ccacggtggc ctggcactgg cgaatactct ggcaaccgca ctggaggctg ccgccctgtt
     8701 ggtgcttctg cggcggcaaa tgaaaggcct gaatggccgg gccattggtc aggttacagt
     8761 gcaagctgga ctggctgcca ctgcaatggc gttggggttg gcgctctggt tgatggctac
     8821 gcgcaatatt ccaaactgga tacaggctgt ggggggcgtg gctgtgggca gcggcttgta
     8881 tggcctgctg ctctgggggc tgcgggttca tgaagtatca cgggtaatgg cggcagtgcg
     8941 ccgacgcctg ccgggacgtt ttcagagttg atttcagaat tcttgaggag gtttttccaa
     9001 tgacaaaaaa cagtggtcac gactttatgc agcgaacgcg ttataccgaa atgggaccct
     9061 cggcacagag ccagggcttg ccgcaaccag cattggaaac agattatccc ctgcaggcaa
     9121 aactgatctc cctgccggac ccggcggaac tgacaatgcc tggcgttgat ttgcggcggg
     9181 caattgagga gcgcgtctcc atccgacaat acagccagga gcccctgacg attgaagaac
     9241 tgacgtacct gctgtggacc acgcagggcg tgaaagccgt aaccgcccgc ccggtgacgc
     9301 tgcgaacggt gccatcggca ggtgcgcgcc atgcctttga gacttatctg ctgatcaacc
     9361 gggttagcgg gattgagccg ggactgtacc ggtatctggc aattgaaaac gcgctgctgc
     9421 ccatcaccat gggccccgaa attgccaccc aactgaccga agcctgcctg aaacagcaga
     9481 tgattaacac cagcgcggtg acctttttct ggacggcggc agtagagcgg atgttctggc
     9541 ggtacggcga acgcggctac cggtatctgc atctggatgc cggacacgtc tgccaaaacc
     9601 tctacctggc agcctgggaa atcaactgcg gcacgtgtgc gattgcagcg tttgatgacg
     9661 acatgctcaa tgcagcgctg ggtttggatg gagagacaac gttcacggta tatctggcag
     9721 ccgtgggacg taaagggaaa tgctaatcct tcccgaaggc ataccggtca atcacgagta
     9781 aaaaacaggt ggtcaatctt gaggattgac cacctgtttg ctgacggata accgggtttg
     9841 aggcttactt gcggcgcttg atgaagcggt agatcacgat gacgatgatg gcacccgcca
     9901 gagccagcag gatgctggtg aggttgatac ctgtcatcag gtcgaggcca aagaggctgc
     9961 cgatgagacc gccaacgatg ctgccgacca cacccaggat gagttcaacc cagatgctgg
    10021 tattggtttt catgatcttt gaggccagga aaccggcaat caggccaacg acaattgttg
    10081 caaaaaggtt catggtgtat ctccta
//
LOCUS       DAOPVS010000065        10082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6732_length_10082_cov_10.123068, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000065 DAOPVS010000000
VERSION     DAOPVS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6732_length_10082_cov_10.123068"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..527)
                     /locus_tag="PKL11_06735"
     CDS             complement(48..527)
                     /locus_tag="PKL11_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ13336.1"
                     /translation="MSLVCVPAGDFMMGVDGGVYDDERPKHTVYLDAYWIDQTEVTNA
                     QYARCVAAGACREPESKDSNTRDSYYGASQYADYPVISVSWSNAADYCSWAGRQLPSE
                     AQWEKAARGTDGWLYPWGYESPSANLLNYDRNSGDTTVVGSYPDGASPYGALDMAGC"
     gene            1014..2864
                     /locus_tag="PKL11_06740"
     CDS             1014..2864
                     /locus_tag="PKL11_06740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNZ13337.1"
                     /translation="MPTFQPDDIVLRDYKIETFIGEGGFGEVYQARDLNLNEPVALKI
                     LRRTAETDAAYYERARDRFTLEARLGHRITHPNIIRVYKFAPDDTSGLLVLVMEYAPG
                     GSLADKLKSGLPAVPDALWIARQVAAGLGALHHADVVHRDLKPSNILFDATGTARVAD
                     LGLAQPASAIQVSQSSSGGGQLYTPPGTPAYMSPEQEGGRMQHLPPASDIYALGLILF
                     EMLTGRTYKNQPPGTRLSQLRPEIPLVLDELVAAMLADDPKERPWDGAAAEKALQELS
                     ADEPVAPPVDAIPARTAATSSRVSATPTWTLEEGLSALKDMEEAQDWQLASELLDELE
                     AAYPNHPKLKLPHKKIPQAEERARREAEEQELREIEEMDRREAEEKANRYMRRDGDRI
                     LIKLDAQQEMVFVRIPAGKFLMGSNLQNRVTLPEYWMGLAPVTNAQYWAFILEEGRKA
                     PGHWQNNRPPQDKMNHPVVNVDSFDAYYYCKWLHYLTGLDVALPKEEEWEKAARGTDG
                     RIYPWGDQAPDAKHCNFNQNVKDTTPVGRYSPVGDSPYGCVDMAGNVWEWCADSVYHG
                     QVMRGGSWRDDLWQVRATYSRSYNGSHYADDLGFRCLLSLNSELLGFWDA"
     gene            3144..5042
                     /locus_tag="PKL11_06745"
     CDS             3144..5042
                     /locus_tag="PKL11_06745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNZ13338.1"
                     /translation="MPIFKPGDIVLRDYQIEAFIGEGSFGEVYRAADLNLKETVALKI
                     LRRSAGMADGYYERARDRFTLEARLGHRITHPNVIRIYKFAPDETSGLLVLVMEYAPG
                     GSLADRIKSGPLAVSDTLRIARQVAAGLGALHEQDVIHRDLKPSNILFDSTGTAKVAD
                     LGLAQPANAIQVSESSFGGSRELHTSPGTPAYMSPEQESGKPHLPPASDVYSLGLILF
                     EMLTGRSYKNQPPGKRVRTMRSDVPATVDELLVKMLSEDPRRRPWNGAAAEKALSKLL
                     KTVSARTSSSHKFAKALLIVGAVALLVGLGPSAAQTLNWLPGHRPTAVTGLDRTTASP
                     QTSVTASDPNTPPACTQINQAWISPIDGMTLVCVPAGEFLMGSTNEDADDDEKPQHTV
                     YLDAYWIDQTEVTNAMFAEFVAAKGYRATGEEEGSGVVYTGSEWTWVDGANWQHPSGP
                     GSDLTGKEDHPVVQVNWDDAAAYCAWAGRQLPSEAQWEKAARGEDGRLYPWGNADPTG
                     NRLNFADINTNFDWSDKAIDDGYEDTAPVGSFPAGVSPYGMLDMAGNVWEWTAEWYGI
                     NYYSSQTTWRNPVGPVSGDYRMVRGGSWYSNTWYVRTAGRLWRTPDLRDNNLGFRCSF
                     STAPLIGP"
     gene            complement(5067..7226)
                     /locus_tag="PKL11_06750"
     CDS             complement(5067..7226)
                     /locus_tag="PKL11_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ13339.1"
                     /translation="MNQEPEARTLGDYIIHDEIGRGGFAIVYRAEDRNLHKIVALKLL
                     NPALFNDAGITERFICEARSAAQLVHPNIVRVTDLDEKEGRIFMVLDYLPGGDLAAWQ
                     ARHGRLSLQQGLAVLTDVAAALDYAHQQGIVHGDVKPQNILLAGRSENNNPGQARLTD
                     FGLLRAVEASGASTTTTELVAGTPYYLSPEQARGERPGPASDLYGLGVVAYELFTGQV
                     PFKDGTPVAIYRQHLMNTPPDPQSLNPQLSEEFAAILMRALAKAPEERYSNCGELVRA
                     LAQAATAMQKDRFGDLVHTARQALADNDLATARARLKDALLLAPTDPTTQQMLKNLES
                     QETAADWYARSAASLGTARQQAAELQATAPNTPDPQELLVKLSPPLLPPLKQFWQNYR
                     LSILAGLGILILALLLGMGTRLAAWGSEGSRATVIALVHTYTHTPTPTSTATFTPTFT
                     PTPTNTLTPTPTPTSTLVPPPCMETGQIWISPLDGMRMVCVPASYFLMGSLDDQYGYP
                     DELPQHVVYLGAYWMDQTEVTNAQYTQCVADGACREPENHRSWTRSFYYGNDQFANYP
                     VIYVSWDDAKNYCTWAGRYLPSEAQWEKAARGIKGLVFPWGNQEPDINLLNSYGNITD
                     TTVVGNYPDGSSPYGVLDMAGNIWEWTADWYSDSYYSSQISWRNPVGPNSGKFRVIRG
                     GSWNNEAIYVRTAYRGWLSPDNRNDSLGFRCILSETH"
     gene            complement(7249..8460)
                     /locus_tag="PKL11_06755"
     CDS             complement(7249..8460)
                     /locus_tag="PKL11_06755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ13340.1"
                     /translation="MSQLGKYELHEKLGEGGFATVYRATHLTLGSEAAVKVLTLTDTE
                     SSARQRFIREAQVAAGFEHPNIARVLALGEQGEQVYIVMEYFPEGDLTRWVKNHGRLT
                     RAETLRILGDIAAALDYAHARQAWHRDVKPGNILMDVNESAHLTDFGLVSVPNEPHLT
                     QIGGVVGSPAYMSPEQAEGRELNGAADQYSLTVVAYELITGKLPFTAESSTSVALMHL
                     TQTPPLPSQVNPDVPTEVDAVLMRGLAKKPTERYATCTDLVRSLETAFQESDARRFRA
                     LISEAREHLKAGRADEAHSLMAELTRLAPAGPQNSALLQAMEVEIERARQYAVLVKNW
                     ETARQQGTEALERFPNLPDPEGIFPILGLRPKPPLPFKRVLREVGIGLLVGLIPALGL
                     LILFWLWIDKV"
     gene            complement(8488..9942)
                     /locus_tag="PKL11_06760"
     CDS             complement(8488..9942)
                     /locus_tag="PKL11_06760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ13341.1"
                     /translation="MKQLGKYEIIELIGKGAYAEVYRAVDTTLDRTVALKLLKPALLA
                     DEDAVGRFMREARTAANLIHPRIAWVWEVGELEGRFYIAMRFIEGQPLDQFLKSQGPL
                     PWAQAQEIIQQIGEALSYAHGRGLVHRDVKPQNVLLSKDEGAILTDFGLVRAMESSGV
                     GTRTGVLMGTPAYMAPEIWRGENAGPAADQYALACILCELLTGQQLFGAANTPAVILR
                     HMQPPQLPEQWPANCSPNLSLTLGKALDPQPDQRYGSVADFIAMVATAEEPGTAPASN
                     DTQSGNQIGQAQNPNIPADDQASEPEQFKPLELRVRARQKSATKPTQANQSPKHNPAE
                     LAKSGHQRPYWPALLWIALGWTPSALFVFDMWGIINLGISYSLPLAILAFAIAAGLGW
                     TITGLAIKKQYQLESKHIVRLLLLGIGSGIIGWILGEIFSYLIFRVFVPFSVLSLLLT
                     SFISFLIMGAASGAIMIAILEAARKRAARQTETN"
BASE COUNT         2164 a         2827 c         2827 g         2264 t
ORIGIN      
        1 tcatctgtgc gacatgatcc cggttaagct gtgcgttttc gccggattca gcagcctgcc
       61 atgtccaacg cgccatacgg gctggctcca tcagggtaac tgcccacaac cgtcgtatcg
      121 ccactgttcc tgtcgtagtt cagcaggttg gcactgggag attcatagcc ccaaggatac
      181 agccagccat ccgtcccccg ggccgccttc tcccactggg cttcacttgg caactgtcta
      241 ccagcccaag agcagtaatc agcggcatta ctccaagata cggaaatcac cggataatca
      301 gcgtactgac tggctccata ataactgtca cgcgtgttag agtctttgct ttcaggttcc
      361 ctgcaggctc cggcagccac acagcgggca tattgggcgt tggtcacctc ggtctgatcg
      421 atccagtagg catccaaata cactgtgtgc ttcggccttt catcatcata tacaccccca
      481 tccacaccca tcatgaaatc acccgccggg acacacacca ggctcattcc atccaatggc
      541 gatgtccagg tctggccgat ttcggtgcag ggcgggggga tcaacgttga ggtcgggtga
      601 tttgtcggcg ttgccacatg tctggcagat tcccacgcct tcccagtcag cggcatccgg
      661 tgtttagctg ctggatgggg ctggggcttc tgacagcaac ccggtgggct ggccacctga
      721 aagctcccag ccggagccgg gctctgggat ggcatcgggc gagccgggcg gttaaaattt
      781 cgcagctgcg ggtgcggccc atgccggcca gtaccaacac cagatccggg gtgggcgcgg
      841 gacaaggacg gtatcggtaa atgcgatggc ggtggcgctc ccgattgcag ccggagcggc
      901 gactttgggt gacggcggcg tttatcctgc tcgctgtggc cccgcctttc ccggtgatga
      961 tggtataata ttttcatcaa tcaaccaaca accaaccgcc tgggggatta atcatgccca
     1021 cctttcaacc tgatgacatt gtcctgcgag actataaaat cgagaccttt attggcgaag
     1081 gcggttttgg tgaggtttac caggcccgcg atctgaacct gaacgagccg gtggcgctca
     1141 agatattgcg ccgcacggct gaaacagatg ccgcgtatta cgagcgcgcc cgggaccgct
     1201 tcaccctgga agcccggctg ggccaccgca tcacacatcc gaacatcatt cgggtctaca
     1261 aatttgcccc ggatgacacc tcggggctgt tggtgctggt gatggaatac gcccccggcg
     1321 gctccctggc cgacaaactg aaatccggcc tgccggcagt gcccgatgcg ctgtggatcg
     1381 cccggcaggt ggcggccggt ttgggtgcgc tgcaccatgc ggatgtggtt caccgggacc
     1441 tgaaacccag caacattttg tttgatgcca ccggcactgc ccgggtggcc gatctgggtt
     1501 tggcccagcc ggccagcgcg atccaggtca gccagtccag ttccggcggc ggccagctgt
     1561 acaccccgcc cgggacaccg gcctacatga gcccggagca ggagggtgga cgcatgcagc
     1621 atttgccccc ggcctccgac atttacgcgc tgggcttgat cctgtttgag atgctgaccg
     1681 gacgcacgta caagaaccag ccgccgggga cacggttgag ccagctgcgc ccggaaatcc
     1741 cgctggtact tgacgagctg gtggcggcca tgctggctga tgatcccaag gagaggccct
     1801 gggatggggc ggcggctgaa aaggcgctcc aggaactgtc ggctgacgag ccggttgcgc
     1861 cacccgtgga tgcgattccg gcccgcactg ctgccacctc ctcccgcgtc tcagccaccc
     1921 ccacctggac cttggaggaa gggctgtctg cgctcaagga tatggaagag gcgcaagact
     1981 ggcagctggc gtcagaacta ctggatgagc tggaggccgc ctatcccaac catccaaaat
     2041 tgaaactgcc acataaaaag atcccccagg ccgaagaacg cgctcggcgt gaggcggagg
     2101 agcaggagtt acgggagatc gaggaaatgg accggcgtga agccgaggag aaagccaatc
     2161 ggtatatgcg tcgtgatggc gaccgaatcc tcatcaaatt ggatgcgcag caggaaatgg
     2221 tttttgtccg gatcccagcc ggtaaatttt tgatgggcag caatctacaa aaccgcgtca
     2281 ccctgccgga gtactggatg gggcttgcac cggtgacgaa tgcccagtac tgggccttta
     2341 tcctggagga gggaaggaag gcaccgggac actggcaaaa caaccggccg ccgcaggaca
     2401 aaatgaatca tccggtggtc aatgtggact cgtttgatgc ctattattac tgtaagtggt
     2461 tgcactacct aactggcctg gacgtggccc tgccaaagga agaggagtgg gaaaaagcgg
     2521 cccgggggac ggatggccgg atttacccgt ggggtgacca ggcaccggat gcgaagcact
     2581 gtaacttcaa ccagaatgtt aaagatacca ccccggtggg ccgatacagt ccagtcgggg
     2641 acagtcccta cggctgtgtg gacatggcgg gcaatgtctg ggagtggtgt gccgacagcg
     2701 tttaccatgg gcaagtgatg cgcggcggtt cctggcgcga tgatttatgg caggtgcgcg
     2761 ccacctacag ccgctcttac aatggttccc actatgccga tgatctgggc ttccgctgcc
     2821 tcctctcgct caattctgaa cttctgggat tctgggatgc gtgacctgaa ggtcactaac
     2881 ctaacacgat ggaagtagcg tgacctgaca atgccctgtt tggtcaggcg gcctgagacc
     2941 aggcgggcca gggtaagtgg ggcatatcgt accaagtctg acaaagcatc gaaacccagc
     3001 gtaggatgca tagccgcccg cgagctgcca caaaagcgaa ccattgaaga gtcatcatga
     3061 aacagaagcc gccccggttt tataagcaac ttaatgatgt caaaatgata taatcacctc
     3121 agtccaatca caggggttct tccatgccca tctttaagcc cggcgacatt gttctgagag
     3181 actaccagat cgaagccttc atcggtgaag gtagttttgg cgaagtctat cgggcggccg
     3241 acttgaattt gaaggaaact gtagcgctca agatcttgcg ccgctctgcc gggatggcag
     3301 atggctatta tgagcgtgcg cgggatcgct ttacattgga agcccggctt ggtcaccgca
     3361 tcactcatcc gaacgtcatt cggatctata agtttgcccc agacgaaact tctggattgc
     3421 tggtattggt gatggagtat gctcccgggg gctcgctagc ggatcggatc aaatcagggc
     3481 cgttggcagt atcggacaca ctgcgaatag cacggcaggt ggcggcaggt ctaggcgcct
     3541 tgcacgaaca ggatgtgatt catcgggatc ttaaacccag caacattctg tttgattcaa
     3601 ccggcacagc caaagttgcc gacctgggac tggctcagcc cgcgaatgcc attcaggtga
     3661 gtgagtccag ctttggcggc agccgggaac tgcacacctc ccccggcacg ccggcttaca
     3721 tgagcccgga acaggaaagc ggaaaaccac acctgcctcc ggcatcagac gtttattcgc
     3781 ttggcctgat cctgtttgag atgcttaccg gacggtccta caagaaccaa ccgccgggaa
     3841 aacgggtccg gacgatgcgg tcggatgtgc ctgctaccgt ggacgagcta ttggttaaaa
     3901 tgctgtccga agatccccgt cggcgaccct ggaatggggc cgctgcggaa aaggctttga
     3961 gcaaattact caagacggtg tctgccagaa catcctccag ccacaaattt gctaaagctc
     4021 tcctgattgt aggggctgtg gcattgctgg tgggcttggg gccgagtgct gctcaaacat
     4081 tgaactggtt gccaggtcat aggcccacgg cggtgactgg tttggaccgg accacggcgt
     4141 caccccaaac gtctgtaact gcatcggatc cgaatacccc cccggcttgc acccaaatca
     4201 atcaggcctg gatttcaccg attgatggga tgaccctggt ctgtgtgccg gcaggcgagt
     4261 tcttgatggg atcgaccaat gaggatgctg atgacgatga aaagccgcag cacacggtat
     4321 acctggatgc ctactggata gatcagaccg aagtgaccaa tgccatgttt gccgagtttg
     4381 tggccgccaa aggttaccgc gctactggcg aggaggaagg ttcaggggtg gtttatacgg
     4441 gcagtgagtg gacgtgggtt gatggcgcca actggcagca tccgtcgggg ccgggcagtg
     4501 acttgactgg aaaagaggat catcccgtgg ttcaagtcaa ctgggatgat gcagcggctt
     4561 actgtgcctg ggcgggccgg cagctgccaa gcgaggccca gtgggaaaag gcggcccggg
     4621 gtgaggatgg ccgattatac ccctggggca atgcggaccc taccgggaac cgattgaatt
     4681 tcgccgatat taatacaaac tttgattggt ctgataaagc catcgatgat ggttacgaag
     4741 atactgctcc agtgggcagt tttccggccg gggtcagtcc ctacgggatg ctggacatgg
     4801 caggaaatgt ttgggagtgg actgcagaat ggtatggcat caactactat tccagccaga
     4861 ctacctggcg aaatccagtt ggaccggtat ccggggatta tcgcatggtg cgcggtggtt
     4921 cctggtacag taatacgtgg tacgtccgta ccgccggccg tctctggcga acccctgacc
     4981 tccgtgacaa taatctcggg ttccgctgca gctttagcac agcaccctta attggcccgt
     5041 aagatcgcaa atgaaagtga accctctcag tgcgtttcac taaggatgca acggaacccg
     5101 agagaatcat ttcggttatc agggctgagc caaccacggt atgccgtacg cacatatatt
     5161 gcttcgttat tccaggaacc gccgcgaatt acacggaact tccctgaatt cgggccaacc
     5221 gggtttctcc agctaatttg actggaataa taactatctg aataccagtc tgcagtccat
     5281 tcccaaatgt taccggccat gtccaaaacc ccgtaggggc ttgacccatc cggataatta
     5341 cccacaacgg tggtatctgt tatgttcccg taactattga gaagattaat gtcgggctct
     5401 tgatttcccc aaggaaaaac aagcccctta atcccacgcg ccgctttttc ccactgcgct
     5461 tcgctcggta aataccgccc tgcccaggtg cagtaatttt tggcgtcatc ccaagataca
     5521 tagattactg ggtaattagc aaattgatca tttccataat aaaatgaccg tgtccatgat
     5581 ctgtgatttt ctggttctcg gcaagctcca tcagccacac actgtgtgta ctgagcatta
     5641 gtcacctcgg tttgatccat ccagtaagcg cccaggtaaa caacatgctg tggcaattca
     5701 tctggatacc catattgatc atctaatgag cccatcagaa aataacttgc tggcacacag
     5761 accattctca taccatctaa tggcgagatc catatctgac cggtttccat gcaaggaggt
     5821 ggtacaaggg ttgaggttgg cgtaggtgtg ggtgtgaggg tgtttgtagg agttggggta
     5881 aaggtaggcg tgaaagttgc tgtgctggtt ggggtggggg tatgcgtgta ggtgtgaacc
     5941 agggcaatca ctgtcgcccg gcttccttcg ctgccccagg ctgccaggcg tgtccccatg
     6001 cccagaagca aagctagtat cagtattccc agccctgcca gaatgcttag cctgtagttc
     6061 tgccaaaatt gctttaaggg cggcagcaga ggcggggata gctttactag caattcctga
     6121 gggtcaggtg tattgggggc tgttgcttgc agttctgcgg cttgttgccg tgctgtgccg
     6181 agactggctg cactgcgtgc ataccagtca gctgcagttt cctgagattc cagattttta
     6241 agcatctgtt gagtggttgg gtctgtaggg gctagcagca aggcgtcttt taacctggct
     6301 cgggcagttg cgaggtcatt atctgccagt gcctgccgtg ccgtatgcac taggtctcca
     6361 aaccgatcct tttgcattgc ggtcgctgcc tgtgccagtg cccgtaccag ttccccgcaa
     6421 ttcgagtaac gttcttctgg ggctttagcc agtgcccgca ttaatattgc ggcaaactct
     6481 tcgcttaact gcgggttcag actttgtgga tccggcggtg tattcatcag gtgctgccgg
     6541 tagatggcca ccggggtgcc atccttgaaa ggtacctgtc cggtaaatag ttcgtaagcc
     6601 accactccca acccatacaa atcgctggct ggaccgggac gttccccgcg ggcctgctct
     6661 ggcgaaaggt aatagggtgt tcccgccacc agttcggtag tggtggtgct ggcccccgag
     6721 gcttccaccg cccgcagcag tccaaaatcg gtcagccgtg cctgcccggg gttgttgttc
     6781 tccgatctgc cggccagcag aatgttctgc ggcttcacat ccccatgcac aatcccctgt
     6841 tgatgtgcat aatctaatgc cgcagccaca tcggtcagca ctgccagccc ctgttgcagc
     6901 gagagccgac catgccgggc ctgccaggca gccagatcac ccccggggag gtaatccagc
     6961 accatgaaaa tgcgtccctc tttttcatcc aggtcggtca cccgcacaat attggggtgc
     7021 accagttggg cagctgagcg agcttcgcaa ataaaccgct ctgtaattcc agcatcattg
     7081 aacagtgccg gatttaacag tttcagcgcc acaatcttat gcagattgcg atcctctgcc
     7141 cggtacacaa tcgcaaaacc gccgcgccca atctcgtcat gaataatata atcgcccaga
     7201 gttcgcgctt ccggttcttg gttcatcgga tcgcctccac agtctctttc atactttatc
     7261 aatccacagc cagaataaaa tcaacaagcc caatgccgga atcagaccaa ccaacaggcc
     7321 aatgcctact tcccgcagca cccgcttgaa tggtaatggc ggtttaggtc gcaatcccag
     7381 gatggggaag atgccctctg ggtctggcag gttggggaag cgttccagcg cttcggtgcc
     7441 ctgctgtcgg gcagtttccc aatttttgac cagtacagca tactgccggg ctcgctcaat
     7501 ttctacttcc atggcctgca gcagggcgct gttttgcggc ccagccgggg ccaaccgggt
     7561 cagttcagcc atcaagctgt gggcctcatc tgcgcgcccg gctttcaaat gctcgcgggc
     7621 ttcgcttatc agcgctcgga accgtctggc atcggactct tgaaaagcag tttccaggga
     7681 gcgcaccagg tctgtacagg ttgcgtagcg ctctgtcggc ttttttgcca gtccacgcat
     7741 cagcaccgcg tccacttcgg tcggcacatc cgggttcact tgactgggca gaggcggtgt
     7801 ctgtgtcagg tgcatcagcg caaccgatgt actgctttcg gcggtaaaag gcagcttccc
     7861 cgtaatcaac tcataggcca ctaccgtcag cgaatattgg tcggctgcgc cgttcagttc
     7921 gcgcccctcg gcctgctccg gtgacatgta tgcaggtgag cccaccacgc cgccaatctg
     7981 tgtcaagtgc ggctcgtttg gcaccgagac gagtccaaag tcggtgaggt gagcgctttc
     8041 attaacgtcc atcaaaatat tgccgggctt cacgtcgcga tgccaggctt ggcgggcgtg
     8101 ggcgtaatcc agcgctgcgg caatatcccc tagaatacgg agagtttctg cgcgggttag
     8161 tcggccatgg tttttgaccc agcgggtcag atcaccttct ggaaaatact ccatcactat
     8221 ataaacttgc tccccctgtt ccccaagggc cagcacccgg gcaatattgg ggtgctcaaa
     8281 tcccgctgcc acctgcgcct cccgaatgaa gcgctgccgg gcgctgcttt ctgtgtcggt
     8341 cagtgtcagc accttcaccg ccgcctcgct gcccagcgtc aggtgcgtgg cccgatatac
     8401 cgttgcaaac ccaccttccc ccagcttctc atgcagttca tatttaccca gttggctcat
     8461 ggtcttgctc ctgccgaggt gaaaatatca attggtctca gtttgccggg ctgcccgctt
     8521 gcgggcagct tccaggatgg cgatcatgat tgcgccgctg gccgccccca ttattaaaaa
     8581 tgatataaaa gatgttaaaa gcaatgacaa cacggaaaaa ggaacgaaca ccctaaatat
     8641 caagtaggag aaaatttcac caagtatcca accaattatc ccactgccga tgcccaataa
     8701 tagtaatctg acgatatgtt ttgactccag ttgatattgt ttttttattg ctaaaccggt
     8761 aattgtccag cctaatcccg ccgcaatggc aaaagccaaa atagctagag gtaatgaata
     8821 agatatgccg agattaataa ttccccacat gtcaaaaacg aaaagagcgg agggggtcca
     8881 tcccagggca atccataaca gagcgggcca gtaggggcgc tgatgacctg atttggcaag
     8941 ttcggccggg ttgtgttttg gggattgatt tgcctgcgtc ggtttggtgg cgcttttttg
     9001 acgagccctg acgcgcagtt caagtggctt gaactgttcg ggttccgatg cttgatcatc
     9061 ggcgggaatg ttgggatttt gcgcctgccc gatctggttg cctgattgtg tgtcgttact
     9121 cgccggtgct gtccccggtt cttcagcagt agcaaccatt gcgataaagt cagccacact
     9181 gccatatctt tgatctggtt gtggatccag cgccttgcca agcgttaaag ataaatttgg
     9241 cgaacaatta gccggccatt gttccggcag ttgaggtggc tgcatatgcc gcaaaatcac
     9301 cgcgggtgtg ttggcggcgc caaaaagctg ctgcccggtc agcagttcac acaggatgca
     9361 cgccagcgcg tattggtctg ctgctgggcc tgcattttcc ccgcgccaga tttccggtgc
     9421 catgtacgcc ggcgtcccca tcaacacccc tgtgcgggtg cccacgccgc tgctttccat
     9481 tgctcgcacc agcccaaaat cggtcagtat tgcgccttcg tctttactca atagaacatt
     9541 ttgcggcttc acatcccgat gcacgagtcc gcgcccatgg gcataactaa gtgcttcgcc
     9601 aatctgctgg ataatttcct gtgcctgtgc ccagggcagc ggcccctggg acttcaagaa
     9661 ttgatccagc ggctggccct caataaaccg catggcaata tagaaacgcc cctccagttc
     9721 acccacctcc catacccagg caattcgggg atggatcagg ttggccgctg ttcgggcttc
     9781 gcgcataaac cggcctacgg catcttcgtc agctagcagc gctggcttta gcagtttcag
     9841 cgccaccgtc cggtccaacg tggtgtcaac cgcccgatat acctccgcgt aggcaccttt
     9901 gccgatgagt tcaataattt catatttgcc aagttgcttc atagaaaatt ctcccggcct
     9961 cgggtttttg gtggggcttt caaaatcccg cctgcaatga ttatatcata acggttttag
    10021 ggcgaataca aaagaggcgg tcaatttgac cgcctctgat ccgaaccctg agacgctatg
    10081 gc
//
LOCUS       DAOPVS010000066         9928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6864_length_9928_cov_60.358959, whole genome shotgun sequence.
ACCESSION   DAOPVS010000066 DAOPVS010000000
VERSION     DAOPVS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6864_length_9928_cov_60.358959"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1383)
                     /locus_tag="PKL11_06765"
     CDS             complement(175..1383)
                     /locus_tag="PKL11_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNZ13342.1"
                     /translation="MFLTHLSLTNFRAFTRLDMDLPRRILLLVGDNAQGKTSILEAVF
                     YLATFTSLHAQSDRQLINFLTAEEALAVARLVADFQRGDRLHRLEVRLIQEPIGPVGN
                     RFRKEILLDGVKRTAQEAMGVFNAVIFLPQMMRIIEGGPDERRRYLNLALSQAMPGYA
                     AALSEYSQALAQRNALLKLLAERGGSPDQLTYWDELIAGRGAVLIAARIQAIQEMERL
                     AARLHQKLTHSEEVLRIHYQPSYDPLPRPEGQYALPIQTAVQRNGFTIEQIRQGFLAQ
                     LQARRTEELARGVTAIGPHRDEMRFLSNGIDLGDFGSRGQIRTALLALKMSEAAWLHE
                     KTGQWPVLLLDEILAELDTQRRTDLLETLGEGEQALLTTTDTKLFSPTFLEQAARWEV
                     RAGQVVKAVG"
     gene            complement(1385..1894)
                     /gene="def"
                     /locus_tag="PKL11_06770"
     CDS             complement(1385..1894)
                     /gene="def"
                     /locus_tag="PKL11_06770"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ13343.1"
                     /translation="MSLKEIVALPHPVLRRKAHKITVFDKDLQALIEDMIETMRKAPG
                     VGLAAPQVAEPVRLIVVEYTTDEEDENAPTKLYVVANPEIVEMSEEKVTGVEGCLSVP
                     GLIGEVERSESIVVKGQNRRGQLVRIKAQGWLARIFQHEIDHLEGVIFTDRATRIWQP
                     KEGEELPLD"
     gene            complement(1968..3614)
                     /locus_tag="PKL11_06775"
     CDS             complement(1968..3614)
                     /locus_tag="PKL11_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ13344.1"
                     /translation="MSDFSADELNLIRSMHLFRGLSEAEWARVQRDFTILPLKEGEGI
                     FRQGQYADTFYIVLSGRVRLIQSKNGVESDLEILDAGMTFGEEALARRQSYRVRAEAM
                     APTRVLRWQIEDLLLAERNMPTLRPFMDTLRETYDLMVTQSMPWRGPREVVYLMARRH
                     SIFLWLKLIPPLLLGAVTSVPLLALYAARAQNPVFLILAILFLLVLAVWVGLIWLDWA
                     NDFSIITNRRVLFHERVILLYDSRREVPLEAVLSDDVTTSFLGRMFGFGTVIVKTYTG
                     KLEMPDLRYPNVVRAILGVQRERAKVRQERNRRRNMDDTIRQRFQHLPPLPVPEEETY
                     VAPIVQPGRLQIALSDFFQLRRVVGDTITYRTHWLILFAHAWAPTLILTLLFLGLLLR
                     IFNLFTFIPLAAVFGLWLFFSIGFGLWWLYCFIDWRNDLYILNNNELIDYYRKPLGEE
                     NRRSAPLENIQTIDYEQKNIIALLFNYGTVFIRVGNDALSFNNVFNPSEVQREIFERF
                     VEKRRRKEQEAVRAEENRMLDWLEAYHRVVEGEPPPPNAG"
     gene            3697..5178
                     /locus_tag="PKL11_06780"
     CDS             3697..5178
                     /locus_tag="PKL11_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNZ13345.1"
                     /translation="MRNRSLQILPWISVIILLAAVLLTVVQLIRYSRIRASFTPGTKI
                     AGIPVGGLTRVQAADRLVQAYNLPIEIHYGDAIIQAKPASLGFDMDLEVMLAAADQQR
                     VELPFWQSFWDYLWNRLPAAAEVPLSVTITEERLKAYLQSEIASRYDQPAEAAQPIPG
                     TPNFEPGKPGTSLDIERAVILVEDALRSPTARVVNLTYNRTETVRPSLQNLQVLLQQV
                     IDAQEFDGVTEVYLLDLQSYQELNFAYTQGEAIPSDVAFTAASTMKIPILVSVFRRLN
                     EPTPADALTMIEQMIVRSENDPADRLMETYLDTNLGPLQVTDDMQALGLDNTFLAGYF
                     YPGAPLLRRFDTTANLRTDVSTSPDAYNQTTPAEMGALLTDIYQCAQSGGGSLAAVFP
                     GEISQAECRTMIGYLAQNRIGVLIQAGLPEGTQLAHKHGWITESDGVIHTISDAGIVY
                     TPSGNYVLVISIYRTQQLIWDDANELFAGLSQGIYNYFNLSGQ"
     gene            complement(5159..6229)
                     /locus_tag="PKL11_06785"
     CDS             complement(5159..6229)
                     /locus_tag="PKL11_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ13346.1"
                     /translation="MTISAIAFDWGNTLMRVLPEYSGVMADWPRVEAVPGAEPALSAL
                     AQRARLVVATNAADSNAEQVMRALARVGLDAHLERVYTMHELGARKPAAAFFGTLAQQ
                     LGLPAHQLMMVGDEYLADIVGAQRLGWRTVWLNPSGLTAPGLLPLQDAEITSLAELPA
                     LLDDLSLPGYADCLAWLTELNNPAILLAHVQGVAALSYGMAVWLRAAGLTVNPLLAHR
                     GGLLHDLSKLSADGHQHHGEAAAVWLEAHQQPILAEIARRHMLFGLLNPPEAPQTWEE
                     KIVYYADKLVEGSKLVGVPARLKALQQRYGTITPARLAQLMPALNTLEAELCAPLGLT
                     PEGLLERLQQAYGHYLPTARTN"
     gene            complement(6226..>7227)
                     /locus_tag="PKL11_06790"
     CDS             complement(6226..>7227)
                     /locus_tag="PKL11_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13347.1"
                     /translation="MYHRFLPNRYTESTNTKLRLADFQWQLEQLDAAGYSLISLENWL
                     NGNLTVPPGRRPLIFTMDDLFFADQIFLDKAGNPSPKSGLGWHWQFYQQHPDFGFSWA
                     LFFNMGDKLYANQETTDDWFINMPGWEDALSAAIIWCIEHDAAPYNHTYDHVRLDQTD
                     NSLIAWELKKNDEVLRTFIDRAGRGDLKSRLGNIIALPYSIWPASQSGRGVLAAHLTP
                     EGKPVTAILEADYHYRAAFMPAPYAEGFDAYKIPRVAALNDAITLLVDRKAEFPVAAA
                     CALGPMDPGQVSDPAALMAQIQQAVAESRCPAGVYAVSGLLFRAQAGTVEPITLNTP"
     assembly_gap    7228..7327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7328..8895)
                     /locus_tag="PKL11_06795"
     CDS             complement(<7328..8895)
                     /locus_tag="PKL11_06795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNZ13348.1"
                     /translation="MADETFYQTMLNISLQMAQTRDLQPLLEYAMRTVMELVHAEYGY
                     LALMDESGEVDCRVSLNAEGQTITGPERNISLSILKRVIAQKKPLLMQDAVNGIRTTT
                     GLQLRSVICAPLTTPRATIGALYLENRSRLAIFQQQDLDPLQLFAAQAAVFIENSLLY
                     SSLERKVSERTLALERRANEMETVNSIGLAILSDLKLGEVLRAVYQQCKRVAPVDAFY
                     IALYHPKTEDIVFPLFLDDNKPVPTDGVKYTDPGSVTAHIIRSRETVYIPDDSSPDED
                     LPPLFHVGGKTVRAYLGVPMIVRDQIIGAISIQSYEPNTYTDEHIRLLQTIAVQAAIA
                     VEHARLFDAARHAREETEAANRQLQIALQQMETLASTDKLTGAFNRRKFDEIIGPELQ
                     RAQRYQTPLALIIFDADYYKQFNDKYGHHMGDAVLGKLAQIIMREIRSSDIFIRWGGD
                     EFIVLSPGIGLEQGGELANRLRLAISQHNFPIPEAVTISLGVAAYRPGDTPDSLVRRA
                     DDALYRAKAQGRNCV"
     gene            9060..>9928
                     /locus_tag="PKL11_06800"
     CDS             9060..>9928
                     /locus_tag="PKL11_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNZ13349.1"
                     /translation="MTIVAIDIETTGLDAQRDAIIEVAAVRFNGRRVEGEWTSLINPN
                     RPIPPTITQLTGITNDMIRNAPPLRAVIQDLEAFIGSDPVLGHNVRFDLGFLQQQGIL
                     QLNEVIDTYELAAVLIPTAPRYNLGSLGQIMGILLENSHRALDDARLTHAVFMELYDR
                     ALALPLELTAEIVRHSEPLAWDGAWLFQQILRARIRQPVQARQSRQTEFGALFAQLDE
                     ALGQPLMPNETMTPLDPDEVAALLEHGGPFSHYFEGFEHRPQQVEMLRAITQALSEGY
                     HLMVEAGTGTGKS"
BASE COUNT         2093 a         2655 c         3073 g         2007 t
ORIGIN      
        1 acggctggac gggcggttca aattctgggg gggcggctgc gcccgctgca gttggtgcat
       61 atccccggca ttcccgagac gcgctacctg gtggtgatgg aaaaggtggc ccccaccccg
      121 gcaggttacc cgcggcgaac cgggattcca gctaaaaagc cgttgtagag cggtctaacc
      181 gaccgccttg accacctgcc cggcacggac ttcccagcgg gcagcctgct ccagaaatgt
      241 gggggagaag agcttcgtgt cggtggtggt taaaagcgcc tgctcgcctt cgccgagggt
      301 ctccagcaaa tcggtgcggc gctgagtatc cagttcagcg aggatttcat ccagcagcaa
      361 gacgggccac tgaccggtct tttcgtgcag ccaggcggcc tcggacatct tcagcgcgag
      421 gagcgctgtg cggatctgcc cgcgggagcc aaaatcaccc agatctatgc cgttgctcag
      481 gaagcgcatc tcgtcacggt gcgggccgat ggcagtgacg ccgcgggcca gttcttcggt
      541 acggcgggcc tgaagctgtg ccagaaagcc ctggcggatc tgctcaatgg tgaagccgtt
      601 acgctgtaca gcagtctgga tgggcagggc atactgacct tcgggacgcg gcagggggtc
      661 ataagatggc tggtaatgga tgcgcaagac ctcttctgaa tgggtcaatt tttggtgcag
      721 gcgggctgcc aggcgttcca tctcttgaat ggcctggatg cgggcggcaa tcaacactgc
      781 gccgcgtccg gcgatgagtt catcccagta ggtcaactgg tcaggtgagc cgccgcgttc
      841 tgccaacagc ttgagcaggg cgttgcgctg ggccagggcc tgagaatatt cgctaagggc
      901 ggcggcatag ccgggcatgg cctgagacaa tgccaggttg agatagcggc ggcgctcatc
      961 ggggccgcct tcgatgatgc gcatcatctg cggcaggaaa atgacggcgt taaagacgcc
     1021 catcgcttcc tgggcggtgc gtttaacacc atccagcagg atttctttgc ggaaacggtt
     1081 gcccaccggg cctatcggct cttggatcag gcgcacctcc aggcggtgca gccggtcgcc
     1141 gcgttgaaaa tcggcaacca ggcgggccac tgccagcgct tcttcagcgg tgagaaagtt
     1201 gatgagctgg cgatcactct gggcatgcag cgaggtgaag gttgccaggt agaagacggc
     1261 ttcgagaata ctggttttgc cctgggcatt atcgccgacc agcaaaagaa tccgccgggg
     1321 caggtccatg tccaggcggg tgaaggcacg gaaattggtc agcgataggt gggtaagaaa
     1381 catgtcaatc caggggcagt tcttcgcctt ctttgggctg ccagatgcgg gtggcccggt
     1441 cggtgaaaat cacaccttcc agatggtcaa tctcatgctg gaagatgcgc gccagccagc
     1501 cctgagcttt gatgcgcacc agttgaccgc ggcggttctg gcccttgacc acgatggatt
     1561 cggagcgttc aacctcgcca atcagtccgg ggacagagag gcagccctct acgccggtga
     1621 ctttttcttc agacatctca acgatttcgg ggttggcaac cacatagagc ttggtggggg
     1681 cgttttcgtc ttcctcatcc gtggtgtatt cgaccacaat caggcgaaca ggttcggcaa
     1741 cctgcggcgc cgccaggcca acgccggggg ctttgcgcat ggtttcgatc atatcttcaa
     1801 ttaacgcttg caggtctttg tcaaaaacag taattttatg agctttccgc cgcaagacgg
     1861 gatgcggcag agcaacaatt tctttcagac tcatgtcagt ttccttctac aataattcat
     1921 cgcccctatt gtaccttaga ctgaggcaga gttgaatagg aaaccgctca gccagcgttg
     1981 gggggcgggg gctcgccttc gaccacgcgg tgataggctt cgagccagtc gagcatgcgg
     2041 ttttcttcgg cacggacggc ttcctgctct ttgcggcggc gtttttcaac aaagcgctca
     2101 aagatttcgc gctggacttc agaaggattg aagacgttgt taaagctcaa tgcgtcattg
     2161 cccacacgaa taaatacagt accataattg aacaacaggg caataatatt tttctgttcg
     2221 taatcaattg tttgaatatt ttccagcggg gcagaacggc ggttttcttc accaagcggc
     2281 ttgcggtagt aatcgatcag ttcgttgttg ttgagaatat agaggtcatt gcgccagtca
     2341 ataaagcaat acaaccacca gaggccgaaa ccgatgctga agaacagcca cagtccgaac
     2401 acagcggcca gcggaatgaa ggtgaataga ttaaaaatcc ggaggagaag acccaggaac
     2461 aggagcgtca gaatcagggt tggcgcccag gcatgggcaa agaggatcag ccaatgggtg
     2521 cggtaggtaa tcgtatcgcc gacgacgcgc cggagctgga aaaagtctga aagcgcaatc
     2581 tgcaggcggc ccggctggac gatgggggca acgtaagttt cttcttcagg cacgggcagc
     2641 ggcggcagat gctggaaccg ctggcggatg gtgtcatcca tgttgcggcg gcggttgcgt
     2701 tcctggcgga ctttggcgcg ctcacgctgg acgccgagga tggcgcgcac cacgttgggg
     2761 tagcgcaggt cgggcatttc cagcttgccg gtataggttt tgacaatgac ggtgccgaag
     2821 ccaaacatcc gccccagaaa cgaggtggtg acatcatcgg agaggacggc ttccagagga
     2881 acttcacggc ggctgtcgta cagcaagatg acacgctcat ggaacaagac ccggcggtta
     2941 gtaataatgg agaagtcgtt agcccagtcc agccagatga ggccgaccca gaccgcaagc
     3001 accagcaaaa acaagattgc cagaattaga aagacagggt tctgcgcccg ggcggcgtag
     3061 agcgccagga gcggcacact ggtcacggcg ccgagcagca gtggggggat gagtttaagc
     3121 cagaggaaaa tcgaatggcg gcgggccatc agatagacca cctcacgcgg gccgcgccag
     3181 ggcatggact gcgtcaccat cagatcgtag gtctcgcgca gggtatccat gaaggggcgc
     3241 agggtgggca tgttgcgttc ggcaagcagc aggtcttcaa tctgccaacg cagcacccgg
     3301 gtgggcgcca tggcttcggc gcggacgcga taactttgac gacgggcgag cgcctcctca
     3361 ccgaaggtca tgccggcatc cagaatctcc agatctgatt ccacaccatt tttagactgg
     3421 atcaggcgca cgcgcccgct gaggacgatg taaaaagtat cggcatactg accctggcgg
     3481 aagatgccct ccccttcttt gaggggtaag atggtgaaat cccgctggac ccgagcccac
     3541 tccgcctcac tcagaccgcg gaagaggtgc atactgcgga tcaggttcaa ttcatcggcg
     3601 gagaaatcgc tcataggtta agtatatccc aatttcagat tcggcttcgt cggaaagggc
     3661 aaaactctgg tagaatttgc ttttgcggag agccttatgc gtaatcgcag tctacaaatt
     3721 ttgccgtgga tatcggtcat cattctgctg gcagccgtac tgctgaccgt ggttcaactg
     3781 atacggtata gcaggattcg tgccagcttt acaccgggca ccaaaattgc cggtattccg
     3841 gtggggggat tgacgcgtgt gcaggcagcc gaccgtctgg tgcaggccta taacctgcca
     3901 attgaaattc attatggcga tgccatcatt caggccaaac ccgcctcgct gggttttgac
     3961 atggacctgg aagtgatgct ggctgccgct gaccagcaga gggtagagct ccccttctgg
     4021 caatcgttct gggattatct gtggaaccgt ctgccggctg cggctgaagt tccgctgagc
     4081 gtgacgatta ccgaagaacg gttgaaggct tatctgcaaa gcgaaattgc atcgcggtat
     4141 gatcagccag ccgaggcggc ccaaccgatt ccggggacgc ctaattttga acctggtaag
     4201 ccgggaactt cgctggatat tgagcgggca gtgattctgg tagaagatgc actgcgatca
     4261 ccgaccgcgc gggtggtcaa cctgacctat aaccggactg aaacggtacg gccttcgctg
     4321 caaaacctgc aagtgctgct gcagcaggtg attgacgctc aggaatttga cggtgtgacc
     4381 gaagtgtatt tgctggattt gcaaagctac caggagctca atttcgccta tacgcaaggc
     4441 gaagcgatcc catccgacgt ggcttttacg gcagccagca ctatgaaaat cccgattctg
     4501 gtttcggtgt tccgccggtt gaacgagccc acccctgccg atgcactgac catgattgaa
     4561 cagatgattg tgcgctcaga gaacgatccg gctgaccgcc tgatggaaac gtatctggat
     4621 accaacctgg ggccgctgca agtgacggat gacatgcaag cactgggcct tgacaacacc
     4681 ttcctggcgg gatattttta ccccggtgcg ccgctgctgc ggcgttttga cactaccgcc
     4741 aacctgcgaa ccgatgtatc cacctcgccg gatgcttata atcagaccac cccggctgaa
     4801 atgggcgccc tgctgacgga tatttatcaa tgtgcgcaga gcggcggcgg ctcgctggcg
     4861 gcagtttttc cgggtgaaat ttcacaggct gagtgccgga cgatgattgg ctatctggcg
     4921 caaaaccgga tcggcgtttt gattcaggca gggctgcccg aaggcacgca gttggcgcat
     4981 aaacacggct ggatcaccga atcggatggc gtgattcaca cgatatcaga tgcggggatt
     5041 gtctacacgc cctcgggcaa ctatgtgctg gtcatttcca tttaccgcac gcagcagctc
     5101 atctgggatg acgccaatga gctgtttgcg gggctgtcgc agggtattta caactacttc
     5161 aatttgtccg ggcagtaggc agatagtgcc cgtaagcctg ctgtaagcgc tccagcagcc
     5221 cttccggcgt caggccaagg ggggcgcaga gttccgcttc cagggtgttg agggcgggca
     5281 tcagttgagc cagtcttgcg ggggtaatgg tgccatagcg ctgctgcaag gctttcagcc
     5341 gagccgggac gcccaccagc ttgctgcctt ccaccagttt gtccgcataa tagacaatct
     5401 tttcttccca ggtttggggg gcttcggggg gattgagcag gccaaagagc atgtggcgcc
     5461 gggcgatctc tgccaggatg ggctgctgat gggcttcgag ccagacggcg gcagcttcgc
     5521 catgatgctg gtgcccgtct gcggagagct tgctcagatc gtgcagcagc ccgccgcggt
     5581 gcgccagcag ggggttgacg gtaaggcccg cggcacgcag ccagaccgcc atcccataac
     5641 tgagggcggc aacgccctgc acatgcgcca acagaatggc ggggttgttc agttcagtca
     5701 gccaggccag acaatcagca tagccgggga gcgagaggtc atccagcaag gcggggagtt
     5761 ctgccagaga ggtgatctca gcatcctgca agggcagcaa gcccggcgca gtcaggccag
     5821 acgggttgag ccagaccgtg cgccagccca gccgctgagc gccgacgatg tccgccaggt
     5881 attcatcgcc aaccatcatt agctggtggg cgggcaggcc caactgctgc gccagggtgc
     5941 cgaagaaggc ggcagccggc ttgcgggcgc cgagttcgtg catggtgtag acacgctcga
     6001 ggtgagcatc cagccccacg cgagccaggg cgcgcatcac ctgttcggcg ttggaatcgg
     6061 cggcgttggt ggcgaccacc agccgggcgc gctgtgccag ggcagacagg gcgggctctg
     6121 ctcctggcac cgcctccacg cgcggccagt cggccatcac gccagaatat tcgggcaaga
     6181 cccgcatgag ggtgttgccc cagtcaaagg caatggcaga aattgtcatg gtgtattcag
     6241 ggtaatcggt tcaaccgtgc cagcctgggc gcggaagagc agcccgctga cggcataaac
     6301 acccgccggg cagcgagact cggcgacagc ctgttgaatt tgcgccatca gggccgccgg
     6361 gtcgctgacc tggcctgggt ccatggggcc cagggcacag gccgcagcca ccgggaactc
     6421 agctttgcgg tccaccagca aggtaatggc atcattcagt gcggcaaccc gcgggatttt
     6481 gtaggcatca aagccttcgg catagggcgc aggcatgaaa gccgcccggt agtgataatc
     6541 ggcttccagg atggcggtga cgggcttgcc ctcgggcgtg agatgagccg ccagaacgcc
     6601 ccggccgctc tgactggccg gccaaatgct gtagggcaat gcaatgatat tgcccaggcg
     6661 ggatttcaaa tcgccgcgcc cggcgcggtc aatgaaggta cgcaaaacct catcgttctt
     6721 ttttagttcc caggcaatca aactgttgtc ggtctggtcg aggcggacgt ggtcgtaggt
     6781 gtggttgtag ggagcggcgt catgctcaat gcaccagatg atagcggcgg agagggcatc
     6841 ttcccagccg ggcatgttga tgaaccagtc atcggtggtt tcctggttgg cgtagagctt
     6901 atcgcccatg ttgaagaaca gcgcccagga gaagccaaaa tcggggtgct gttgatagaa
     6961 ctgccagtgc cagcccaggc cagacttggg ggaagggttg cctgctttgt ccaggaaaat
     7021 ctggtcggcg aagaacagat cgtccatggt gaaaatcagc gggcggcggc ccggcggcac
     7081 ggtgaggttg ccgttaagcc agttttccag cgagatcaga ctgtaaccgg cagcatcaag
     7141 ctgttccaac tgccattgaa aatctgccag gcgcagcttg gtattcgttg attcggtgta
     7201 acgattgggc aggaagcggt ggtacatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnacg cagttgcggc cctgggcttt ggcgcggtag agggcgtcat cggcccggcg
     7381 gaccagcgag tcgggggtat ctccgggacg gtaggccgcc acacccaggc tgatggtgac
     7441 tgcctcggga atagggaaat tgtgctgaga gatggccagg cgcaggcggt tagccagttc
     7501 gccgccctgt tccaacccaa tgccgggcga aaggacaata aactcatcgc ccccccagcg
     7561 gatgaaaata tctgatgagc ggatttcccg catgatgatt tgggccagtt tgcccagaac
     7621 ggcatccccc atgtggtggc cgtatttatc attgaactgc ttatagtaat cagcatcgaa
     7681 aataatcaac gccagagggg tttgataccg ctgggcgcgc tgcagctcgg ggccgatgat
     7741 ttcgtcaaat ttgcggcggt tgaaggcgcc ggtcagcttg tcggtagagg ccagggtttc
     7801 catctgctgc agggctatct gaagctggcg gttggcggct tcggtctcct cacgggcgtg
     7861 acgagcggca tcaaacaggc gagcgtgctc caccgcaatg gccgcctgaa ccgcaatggt
     7921 ctgcaaaagg cggatatgtt catcggtata ggtgtttggc tcgtaactct ggatggaaat
     7981 agcaccgatg atctgatcgc gcacaatcat cggcacaccc agataggctc taaccgtctt
     8041 tcccccgaca tgaaacaaag gcggcaaatc ttcgtccggg gatgaatcat ccgggatgta
     8101 gactgtttca cgggagcgga tgatgtgagc cgtgacgctg cccggatcgg tatatttaac
     8161 accatctgtc gggactggct tgttatcatc cagaaacagc ggaaagacaa tatcttcagt
     8221 cttggggtga taaagggcga tgtagaacgc gtctacggga gccacacgct tgcactgctg
     8281 atagacggcg cgcagcacct cacccagttt caggtcagaa aggatcgcca ggccgatgct
     8341 gttgaccgtc tccatttcat tggcgcggcg ctccagggcc agagtgcgct cgctgacttt
     8401 tctttccaga gaactataaa gcaggctgtt ttcgataaat actgccgcct gagccgcaaa
     8461 caactgcagc ggatcaaggt cttgctgctg gaagatagcc aaacgggagc gattttccag
     8521 atacagtgcg ccaatggtgg cgcgcggggt ggtcaaggga gcgcaaatga ccgagcgtaa
     8581 ctgcaggccg gtggtggtac ggatgccgtt cacggcgtct tgcataagca ggggtttttt
     8641 ctgcgcaatg acgcgcttga ggatggacag actgatgttg cgttccgggc cagtaatggt
     8701 ctggccttca gcgttgaggc tgacgcggca gtccacctcg ccggactcat ccatgagtgc
     8761 gaggtagccg tattctgcat ggaccagttc catgacggtg cgcatggcat attccagcaa
     8821 gggctgcaaa tcgcgcgtct gcgccatttg caaactgatg ttgagcatgg tctggtagaa
     8881 ggtttcatcg gccataggaa atacccccag gctgcacacg aaacgtgcaa ggcaatttga
     8941 agctcaagtt ctgcctttcc aatttcatta aattatattt tagcataaag caagtcgaac
     9001 ctgtgcagca tctgcacccg gtttagaggt ttgccgcctg ccggtgatat aatcggcgca
     9061 tgacgattgt agccattgat attgaaacga ctggcctgga cgcacagcgc gatgccatca
     9121 tcgaagttgc ggcggtgcgg tttaatggcc gccgggttga aggggaatgg acctcgctga
     9181 tcaaccccaa ccgccccatt ccaccgacca tcacgcagtt gaccggcatc accaacgaca
     9241 tgatccgcaa tgcgccgccg ctgcgggccg tgattcaaga cctggaagca tttatcgggt
     9301 ctgatccggt gctggggcat aatgtgcgct ttgacctggg ctttttgcag cagcagggta
     9361 ttctgcagct taatgaggta attgatacct atgagctggc tgcggtgctg atacccaccg
     9421 ccccgcggta caacctgggc agcctgggcc agattatggg gattctgctt gaaaattctc
     9481 accgggcgct ggatgatgcc cggctgaccc atgctgtttt tatggagctg tatgaccggg
     9541 cactggcact gccgctggaa ctgactgccg aaattgtgcg gcacagcgag ccgctggcct
     9601 gggatggcgc ctggctgttt cagcaaatct tgcgggcgcg catccgccag cccgtgcagg
     9661 ctcgacagag ccgccaaact gaatttgggg cgctgtttgc gcagttggat gaggcgctgg
     9721 ggcagcccct gatgcctaac gagaccatga cgccgctgga tccggacgag gtggcggcac
     9781 tgctggagca cggcgggccg ttctcacatt actttgaagg ctttgaacac cgccctcagc
     9841 aggtggaaat gctgcgagcc attacccagg cgttatcaga aggctatcac ctgatggtgg
     9901 aggctgggac cggcacgggc aagtctta
//
LOCUS       DAOPVS010000067         9928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6865_length_9928_cov_57.161754, whole genome shotgun sequence.
ACCESSION   DAOPVS010000067 DAOPVS010000000
VERSION     DAOPVS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6865_length_9928_cov_57.161754"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1707)
                     /locus_tag="PKL11_06805"
     CDS             complement(52..1707)
                     /locus_tag="PKL11_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ13350.1"
                     /translation="MKQRSLFRNALFTAFLLLSLVLGGCQAINPYDVQPVDVYTTLKV
                     SSPNCDYGGEFRSIEALDRYTVKFTLCYPDAALPSKLAFPTFSIQDAEYLYEKGGDSA
                     ALSLETNGTGPYMVEEWVPGTSLKLTANPNYWGVPPRSETLTLRWNPSSVYRRSELLF
                     DRADGIDRPDLNSAIFKGNPNYITYTRPALSIAFLGINRDVPPYNDSRVRLGMAMLID
                     RQRVVANSFPAGAEAAEQFLPRSLTPGHSDVLRWHDYNIKEGTDLLNAAGFNYDQELF
                     LYYTDTSTSYLPSPILVAQEIQAQLADAGLKITTVRMEEREFNAAVEAGELGLFLTGL
                     SAEYPDATNFYDPNFSASSQRYGTPYGDISAALLNAKATSDAKSRQYYYDIVNQKLKE
                     YILIIPLAHANDSAVFQADVTNVIISPLNENFDQMSTPDDQLTFMLESEPTFLWPADE
                     TSNDTMRVARLLYTPLVTYAYGSAELAPGLAEYWESNAALTEWTFSLRYGVTFSNGAE
                     LDANDVVATLGAQWNAKDPNHVGRTGSFIYFKRFFGEFINVGK"
     gene            complement(1837..2589)
                     /locus_tag="PKL11_06810"
     CDS             complement(1837..2589)
                     /locus_tag="PKL11_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13351.1"
                     /translation="MRLDVQSLVVRYGLRVALNGVSFSLNSGEVLAVIGPNGAGKTSL
                     LRALSGIQPAESGQMLLDGRDVAKLSPVERARWMAVVPQARQLPALFTGYQTVMLGRT
                     PHINWLGRTLPQDEAAVHQAMQRTGTLELAERRVDELSGGEQQRLLLARALAQGSPLL
                     LLDEPTTHLDLHYQISLMDNIRSLAHDDGLAVLLVTHDLHLVARYSNRVALLVGGELL
                     SQGTPQEVLTPDLLSRAYQIPAEAVNLAALGL"
     gene            complement(2592..>2795)
                     /locus_tag="PKL11_06815"
     CDS             complement(2592..>2795)
                     /locus_tag="PKL11_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNZ13352.1"
                     /translation="HLVRLAWGPDYRHLLPLAAIGGASLLLLADVVARVAMAPQEIPV
                     GIITALLGAPFFLWVLRSSKRGG"
     assembly_gap    2796..2895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2896..3092)
                     /locus_tag="PKL11_06820"
     CDS             complement(<2896..3092)
                     /locus_tag="PKL11_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNZ13353.1"
                     /translation="MPRLSRPYLLNLILLGAALILSVVVGSVWLAPAEIWQWLNGQGG
                     QTAGTILLQLRLPRTLLVAITG"
     gene            complement(3103..4008)
                     /locus_tag="PKL11_06825"
     CDS             complement(3103..4008)
                     /locus_tag="PKL11_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNZ13354.1"
                     /translation="MKKSWLILLVMAILLTACTPKAPTGTTYIDGEGRSIVLAGPAQR
                     IVSLAPSNTEILYAIGAGDQVVGRDEFSDYPAEAKDLPSVGGSMGEYDFEAIAALEPD
                     LVLATAINTPKQVKALEELGITVYMLPNPDDLDGLYANLTTVGQLTGHEAETQVLVDS
                     LKARVQVVLEKVAISSYLPAVFYELDGTDPAKPWTAGPDTFMSKWISLAGGRNVGDEM
                     DSSWAQISQEALIVANPEIILLGDAAYGMTPEQVAARPGWDAIAAVESGKILVFDDNL
                     ISRPGPRLVDALETLARLLHPELFE"
     regulatory      complement(4137..4280)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4397..5485
                     /locus_tag="PKL11_06830"
     CDS             4397..5485
                     /locus_tag="PKL11_06830"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ13355.1"
                     /translation="MISSASPSSPPLSPSLSWDIGSAYDFFVSIYVLHRPADFGLRPS
                     WAAGVRSRLAGPVRDFLEETQNFLTVPLPWLHDLPAVPKTAVVALEALAALPAADRLP
                     ALSFSPETTPELREACAAIAARGSWTNTEADVIRAVFQRRNITPRPGAVQQACQAWSD
                     PAAFGERYLEAVQLYNNVFFREEEARITPALENALTEARALAARLPVADLLDELTRGV
                     HFQAALQLDQLVLAPSFWSAPLVFYRPLQPGRAIMLFGARPADQPLVPGENIPADLVD
                     GLKALADPTRLRILRNLAEGPQTPSELARQVRLRPPTVIHHLNQLRLSGLVEITVQAE
                     GERRYALRQEALAAVIATLKAFIQPESE"
     gene            5482..>5957
                     /locus_tag="PKL11_06835"
     CDS             5482..>5957
                     /locus_tag="PKL11_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13356.1"
                     /translation="MNRILNVLKDYAGNLANFQRNAWLYLIHTILSGAAMGVFRLLFN
                     FYGTSLGYDSQLIGGLITASSFTALIVALPMGLLADKLGRKVSLLTSSLTVASAVMLM
                     ALFPSPGLFIGMNILMGMAQSLGGITMGPFLIENSSDRERVYLFSFASGMNMISGFA"
     assembly_gap    5958..6057
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6058..6627
                     /locus_tag="PKL11_06840"
     CDS             <6058..6627
                     /locus_tag="PKL11_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13357.1"
                     /translation="LILPMLLVSIGAGLTMPFMNLFFRDVHLLSDAAVGSTFAWGSLA
                     MGLGLIIAPPLADRMGKIKLVVLTQGLSIPFLALLGFGPLAVSLTAYYIRLTLMNMSG
                     PVYQTFVMEKVEPSARGTIASLVSMANSFGWAFSPIVSGWIQVRYGFGPAFWGTIILY
                     AAAVFFYWWFFMAPRNQPQSAQLSEAPRI"
     gene            6681..7067
                     /locus_tag="PKL11_06845"
     CDS             6681..7067
                     /locus_tag="PKL11_06845"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNZ13358.1"
                     /translation="MEMVIDFPGGARVDAHFGPFTVATDQPPAATAPTPFALFLSSLG
                     TCAGIYVLGFCNQRGLPTQGIRIIERVHANPLTHKVEKIDLEIQVPPEFPAKYYDALV
                     RSAEQCAVKKLIENPPVFEVTTRVTE"
     gene            complement(7125..8492)
                     /locus_tag="PKL11_06850"
     CDS             complement(7125..8492)
                     /locus_tag="PKL11_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNZ13359.1"
                     /translation="MSTQTTTRFQLNESDLPRTWYNILADAPAAPPPPLHPVTLEPVT
                     PDFLSALFPIGLLEQEMTTERYIDIPEEVREIYKLWRPTPLLRARRLEQMLDTPAHIY
                     YKYEGVSPSGSHKSNTAVAQAFYNKIGGTHALTTETGAGQWGSALAMACQFFGLDLEV
                     YMVCSSYQQKPYRRALMQTYGAQVFASPSDRTEAGRQILAINPQTSGSLGIAISEAVE
                     VAVKSGGMKKYGLGSVLNHVLLHQTVIGEEALKQMDLAGEYPDVVIGCVGGGSNFAGI
                     AFPFLRQNLRDGQRTRLLAVEPTATPSLTRGTYAFDYGDTAGMTPVMKMYTLGHDFMP
                     PAIHAGGLRYHGMAPIISALYDAGLMEAVAVPQRAVFEAALMFNRCEGILPAPESSHA
                     IRAAIDEALAARAAGEKRVILFNLSGHGHFDLSAYEAYMQGDLEDVESMPLELGTIVR
                     QAVAA"
     gene            complement(8528..9568)
                     /gene="trpS"
                     /locus_tag="PKL11_06855"
     CDS             complement(8528..9568)
                     /gene="trpS"
                     /locus_tag="PKL11_06855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ13360.1"
                     /translation="MTARTRILTGHRPTGPRHIGHLVGTLQTWAALQDTRECFFLVAD
                     LHVLTTSETAARIRENTIEMVLDWLAAGIDPARATLVLQSAIPEHAVLGLLLGMLTPV
                     SRLERVPTYKEQIQALGLNPSLGLLTYPVLQTADILLYRAEAVPVGEDQLPHLELARE
                     VARRFNTTYGALFPEPQPVLSRMPRLPGTDNRTMHTSYGNTIYLRDTAAETERKVMQM
                     YTDPSRIHATDPGHVDGNPVFTYLDLFHPEAALVADLKTRYAAGKVGDVEVKRILAAA
                     LNHYLAPLRERRAELAARRYEVLDILYSGTERARPIARATLEDVYAAIGLTDSRSLNP
                     ASHPAAVQGLFC"
     misc_binding    complement(9612..9917)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1860 a         3061 c         2753 g         2054 t
ORIGIN      
        1 aaaaaacagt ccggtgagct tgctcaccgg actgccgcga tgacatgaat cttactttcc
       61 gacgttgata aattcgccga agaagcgctt gaagtagatg aagctgcccg tgcggccaac
      121 atgattaggg tctttggcat tccattgggc ccccagggtt gccaccacat cgttggcatc
      181 cagctcggcg ccgttgctga acgtgacgcc gtaacgcagg ctgaaggtcc actcggtcag
      241 cgccgcattc gattcccagt attcggccaa tccgggcgcc agttcagccg agccgtaggc
      301 gtaagtcacc agcggcgtat agagcaggcg cgccacccgc atggtgtcgt tgctggtttc
      361 gtcagccggc cacaggaagg tgggctcgga ctctaacata aaggtcaact gatcatcagg
      421 ggtgctcatc tggtcaaaat tctcattcag cgggctgatg atgacgttag tcacatcggc
      481 ctggaatacc gccgagtcgt tggcgtgggc cagcggaata atcaaaatat attccttgag
      541 cttctggttg acgatatcgt aatagtactg ccggctcttg gcatcactgg tagctttggc
      601 attcagcagc gcggccgaaa tatccccgta cggggtgccg taccgctgtg aactcgcgct
      661 gaagttgggg tcgtaaaagt ttgttgcatc cggatactcc gcactcaacc cggtcaggaa
      721 cagccccagt tcgcctgctt caacggccgc gttgaattca cgttcttcca tacgcactgt
      781 tgtgatttta agcccagcat ccgccagttg ggcctgaatt tcctgcgcca ccagaatggg
      841 gctgggtaaa tatgatgtgc tggtatcagt gtagtagagg aacagttcct ggtcgtaatt
      901 gaaaccggct gcattgagca ggtcggtgcc ttctttgatg ttatagtcat gccagcgcag
      961 cacatcgctg tgcccgggcg tcagcgagcg cggcagaaac tgctcggcgg cttcagcgcc
     1021 agccgggaaa ctgttggcca ctacccgctg gcgatcaatc aacatagcca tccccaggcg
     1081 cacccgtgaa tcattatagg gcggcacatc gcggtttatc cccagaaagg caatactcaa
     1141 cgccgggcgg gtataggtga tgtagttcgg gttgcccttg aaaatcgccg agttcaggtc
     1201 tgggcggtca atcccatctg cgcggtcaaa cagcagctct gaccggcggt aaaccgaaga
     1261 aggattccag cgcagggtca gggtttctga gcggggcggc acgccccagt aattggggtt
     1321 ggcggtcagt ttgagtgacg ttccgggaac ccactcttca accatataag gcccggtgcc
     1381 gttggtttcc aggctcaggg cagctgaatc gccgcctttt tcatacaggt attcagcgtc
     1441 ttgaatcgaa aaggtcggaa acgccagctt ggagggcagg gcggcatccg ggtaacagag
     1501 cgtgaatttg acagtgtatc ggtcaagcgc ctcaatggat cggaactcgc cgccgtaatc
     1561 gcagtttggt gaactgactt ttagggtcgt gtacacatcc acaggctgta catcataggg
     1621 gttgatcgcc tggcagccgc caagtaccag gcttagcaac agaaaagcgg tgaacagtgc
     1681 gttacgaaaa agagaacgtt gtttcacgtt gattgtccct gggtgaaccg taaatggata
     1741 gtgaccgggc tttaatctca gccctgtttt gattatacgt gttcctgttt gaatgtcaat
     1801 tcaaccccac ttgatatttc tgtaaggata ggggccttac agccccagcg cggctagatt
     1861 cactgcctct gccgggatct gataggcccg gctgagcaga tccggcgtca aaacctcctg
     1921 cggcgttccc tgactcaaca gctcgccccc caccagtaac gccacccggt ttgaatagcg
     1981 cgccaccagg tgcaggtcgt gcgtcaccag cagcaccgcc agcccgtcat cgtgcgccag
     2041 cgagcggata ttatccatca ggctgatctg gtagtgcagg tccaggtgcg tggtgggctc
     2101 gtccaacagc agcagcggtg aaccctgtgc cagcgctcgc gccagcagca ggcgctgctg
     2161 ctcgcccccc gaaagctcat ccacccgccg ctctgccagt tccagcgtgc cggtgcgctg
     2221 catggcctgg tgcaccgccg cctcgtcctg gggcagggtg cgccccagcc agttgatgtg
     2281 cggcgtgcgc cccagcatca ccgtctgata ccccgtgaac agcgccggca gctgccgcgc
     2341 ctggggcacc accgccatcc agcgggcccg ttccaccggc gataacttcg ccacatcgcg
     2401 cccgtccagc agcatttgac cactctcagc gggttgaatc ccgctcaggg cgcgcagcag
     2461 cgaggttttc cccgcgccgt tgggcccaat caccgccaga acctcgccag aattcagcga
     2521 aaaactgact ccgttgagcg ccacccggag cccatagcgt accaccagtg attgcacatc
     2581 cagccgcatg gtcatccccc tcgcttgctg ctccgcagca cccacaggaa aaatggcgct
     2641 cccagcagcg cggtgatgat gcccaccggg atctcctgcg gcgccattgc cacgcgggcc
     2701 accacatcgg ccagcagcag caggctggca ccgccaatgg ctgccagggg cagcagatgg
     2761 cggtaatcgg ggccccaggc cagccgcacc aggtgnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnncccgt gatggccacc agcagcgtgc gcggcaggcg cagttgcagc
     2941 aaaatggtgc cggccgtctg cccgccctgc ccgttcagcc actgccagat ctcggctggc
     3001 gcaagccaca cactgcctac caccacgctg agtatcaacg cggcgccaag caaaatcagg
     3061 ttgagcaggt aagggcggga tagacggggc atgtttgatc cgttactcga ataattccgg
     3121 gtgcagcagg cgcgccaggg tttccagcgc atccaccagc cgcggtcccg ggcggctgat
     3181 caggttgtca tcaaaaacca ggatcttgcc gctttctacc gccgcaatcg catcccagcc
     3241 ggggcgggcc gccacctgtt cgggcgtcat gccgtaggcg gcatcaccca gcagaattat
     3301 ctccgggttg gccacaatca gtgcttcctg gctgatctgc gcccaactgg aatccatttc
     3361 atcccccaca ttgcgcccac ctgccaggct gatccacttg ctcataaagg tatccggtcc
     3421 ggcggtccag ggtttggcgg ggtcggtgcc atccagctca taaaacacag ccggcaaata
     3481 gctgcttatg gcgacttttt ccagcaccac ctgcacccga gccttgagtg aatcgaccag
     3541 cacctgcgtt tcggcttcat gcccggtcaa ctgccccacg gtggtcaggt tggcatacag
     3601 cccatccagg tcatccgggt tgggcagcat gtagacggtg atgcccaatt cttccagcgc
     3661 ctttacctgt ttgggggtgt taatggcggt tgccagcacc aggtcgggtt ccaaagcggc
     3721 gatcgcctca aagtcatatt cacccatcga gccgcccacg ctgggcaggt ctttggcctc
     3781 agccgggtaa tctgaaaatt catcgcgccc caccacctga tcgccagccc caatggcata
     3841 gagaatctcg gtattggaag gcgccagtga gacaatccgc tgcgccgggc cagccagcac
     3901 aatgctgcgg ccttcgccat caatatacgt cgtcccggtg ggggccttgg gcgtgcaggc
     3961 ggtcagcaaa atggccatca caagcagaat cagccaactt ttcttcataa taatacttaa
     4021 actcctttag gggtaaattg aaacaaatct gtcatcaaac aaaagccccg tccaacgtgg
     4081 acggggaggt ataaacgtga agctgccggg cgcttccacc tcctttccgc gagggtgtgg
     4141 tcagcatctc tctgggcggg cgacctggct tgaaccttcg gttctcacag ttgcgcgaca
     4201 gcaccggact tgcaccggtt tcgcctgatt gagggagcct tcccatccgg gggagaggcg
     4261 cccaggagat gtattcaatt gacagcctga ttgtatatgc aacccggcac agcgtcaagg
     4321 gacgatgttt ttttgattta ggggttgaca gttctggcct gttagattat catctaacag
     4381 gttagacgta tatctaatga tatcttctgc ttcaccttct tctccccccc tttctccgtc
     4441 cctctcctgg gacatcggct cggcgtatga cttcttcgtc agcatctacg tccttcatcg
     4501 ccccgccgac tttggcctgc gtccctcctg ggccgcgggc gtccgctcac gcttggcagg
     4561 tccggtccgc gattttttgg aagaaacgca aaatttcctc acagtccctc ttccctggct
     4621 ccacgacctc cctgctgtgc ccaaaactgc ggttgtagcc ctggaagcgc tggcggcgct
     4681 tccagcagcg gaccgcctgc cagcgttgag cttcagcccc gaaactacgc ccgaactccg
     4741 cgaagcctgt gccgccattg ccgcccgcgg cagttggacc aacaccgaag ctgatgtgat
     4801 ccgggctgtt ttccagcgcc gcaacatcac cccgcgccct ggcgcggtgc agcaggcctg
     4861 ccaggcctgg agcgatccgg ccgcttttgg tgaacggtac cttgaagcgg tgcagcttta
     4921 caacaatgtc tttttccgtg aagaagaggc gcgcattacg cccgcgcttg aaaacgccct
     4981 gaccgaagcc cgcgccctgg ctgcccggct gcccgttgcc gacctgctcg atgaattgac
     5041 ccgcggcgtc catttccagg ccgcgctgca gctcgatcaa ctggtgctgg cgccatcctt
     5101 ctggagcgcc ccgctggtct tctaccgccc gctgcagcct ggacgcgcca tcatgctctt
     5161 tggcgcccgc cccgccgacc agcccctggt gccgggtgaa aacatccccg ccgacctggt
     5221 ggatggtctg aaggccctgg ctgaccccac ccggttgcgc attctgcgta acctggcaga
     5281 aggccctcaa accccctccg aactggcccg tcaggtgcgc ctgcgccccc ccacagtcat
     5341 ccatcatctt aatcaattgc gtctctccgg tctggtggaa attaccgtcc aggctgaagg
     5401 ggagcgccgc tacgccctgc gccaagaagc gctggctgcc gtcatcgcaa ccctgaaagc
     5461 ctttatacaa cctgagtctg aatgaaccga atattaaatg tattaaaaga ttacgccggc
     5521 aatctggcga attttcaacg taacgcctgg ctgtacctca tccacactat cctctccggt
     5581 gccgcaatgg gcgttttccg cctgttgttc aacttctacg gcaccagcct gggatatgat
     5641 tcgcagttga tcggtggttt gattaccgcc agcagcttta ccgccctgat tgtggcgctg
     5701 cccatgggcc tgctggctga taaactgggc cgcaaggtct cgctgctcac cagctccctc
     5761 acggtagcca gtgcggtgat gctcatggca ttattcccct cccccggcct gtttatcggc
     5821 atgaatatct tgatgggcat ggcccagagc cttggcggca tcactatggg gccgttcctc
     5881 atcgaaaaca gctctgaccg cgagcgcgtt tatctcttca gcttcgcctc tggtatgaac
     5941 atgatctcgg gctttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc
     6061 attctgccca tgctgctcgt ctccatcggc gccggcctca ccatgccctt tatgaatctg
     6121 ttcttccgcg atgtccatct gctttcggat gccgcggttg gcagcacctt tgcctggggg
     6181 tcgctggcca tgggcctggg cttgatcatt gccccgccgc tggccgaccg catggggaaa
     6241 atcaaactgg tggttctcac ccagggcctc tcaataccct tcctggcgct gctgggcttt
     6301 ggcccgctgg cagtcagcct gacggcctat tacatccgcc tgaccctgat gaatatgagc
     6361 ggtccggttt accagacctt tgtcatggaa aaggtggagc cctctgcccg cggcaccatt
     6421 gccagtctgg tcagcatggc caacagtttt ggctgggcat tcagccccat cgtcagcggc
     6481 tggattcagg tacgctacgg cttcggcccg gcgttctggg gcaccatcat tctctatgct
     6541 gccgcagtct tcttctactg gtggtttttc atggcgccca gaaatcaacc gcaatcggcg
     6601 caactttctg aagctccgcg catctaaatc cgtggtggcg taaaaaacgc ccagatatca
     6661 ctggtggagg atgttgtttt atggaaatgg taattgattt tcccggcggc gcccgggtag
     6721 atgctcactt tggccccttt acggtcgcaa cggaccagcc cccggcggca acggccccaa
     6781 ccccgtttgc tctgtttctc tcttcgctgg gcacctgcgc aggtatttat gtgctgggct
     6841 tttgcaacca gcgcggcctg cccacccagg gcatccgcat cattgaacgg gttcatgcca
     6901 acccactgac ccacaaggtt gagaaaattg acctggagat tcaggtcccg cccgagttcc
     6961 cggctaaata ttacgacgcc ctggtgcgct ctgccgaaca gtgcgcggtc aaaaaactca
     7021 tcgagaatcc ccccgttttt gaagtgacca cccgggtcac agaataaagc ccctgccctc
     7081 cggacgagcc ccctgatctc gcccggaggt tttttatttt cagactacgc cgccaccgcc
     7141 tgccggacga ttgtgcccag ttccagcggc atcgattcga catcttccag gtcgccctgc
     7201 atgtaggctt cgtaggcgct caagtcaaag tgcccgtgcc ccgacaggtt gaacaggatt
     7261 acccgcttct cccctgccgc acgcgccgcc agcgcctcat caatcgctgc ccgaatggca
     7321 tgggatgact ccggcgcggg cagaatgccc tcgcagcggt taaacatcag cgcggcctca
     7381 aatacggccc gctgcggcac tgccactgcc tccatcaacc cggcgtcata cagcgcgctg
     7441 atgatgggcg ccatgccgtg ataccgcagt ccgccggcgt gaatcgctgg cggcataaaa
     7501 tcatgtccca gcgtgtacat cttcatcacc ggcgtcatcc cggcggtgtc gccgtaatca
     7561 aaagcgtagg tgccgcgggt cagagagggg gttgccgtgg gttccactgc cagcaggcgg
     7621 gtgcgctgcc catcgcgcag gttctgccgc aggaacggga aagcaatccc cgcaaagtta
     7681 gacccacctc ccacgcagcc aatcaccaca tccgggtatt cgcccgccag gtccatctgc
     7741 ttgagggctt cttcgccaat cacggtctga tgcagcagca catggttcag aaccgagccc
     7801 agcccatact tcttcatccc gcccgacttg acggccactt ccaccgcttc ggatatggca
     7861 atgcccagcg atccgcttgt ctgcgggttg atcgccagaa tttgccgccc ggcttcggtg
     7921 cgatctgagg ggctggcaaa tacctgcgcg ccataagtct gcatcaacgc ccggcggtaa
     7981 ggcttctgct gatacgatga gcagaccata tatacctcca ggtccaggcc aaaaaactgg
     8041 caggccattg ccagcgccga accccactgt ccggcgccag tttcggtggt caaggcatgc
     8101 gtgccgccaa tcttgttgta aaacgcctgt gccaccgccg tgttggactt atggctgcct
     8161 gatggggaga ccccttcata cttgtagtaa atgtgcgccg gggtatccag catctgctcc
     8221 agccgccgcg cccgcagcag cggcgtgggc cgccagagct tgtagatctc gcgcacttct
     8281 tcaggaatat caatataccg ctcggtggtc atctcctgct ccagcagacc tatggggaac
     8341 agggccgaaa gaaagtctgg cgtcactggc tccagcgtca cggggtgcag gggcggcggc
     8401 ggcgcggcgg gcgcatctgc cagaatgttg taccaggtgc gcggcaggtc agattcgttg
     8461 agttgaaaac gggtggtggt ttgcgtggac atgataatct ccttatcgaa tgaatgcttg
     8521 ggtcaggtta acaaaacagg ccttgcacag ccgccggatg tgacgcggga ttcagcgagc
     8581 ggctgtctgt caggccaatg gctgcgtaaa cgtcttccag ggtggcgcgg gcaatcgggc
     8641 gggcgcgctc ggtgccgctg tacagaatat ccagcacttc gtaccggcgg gcagccagct
     8701 ccgcccggcg ctcccgcagc ggcgccagat agtgattcag cgcggctgcc agaatccgct
     8761 tcacctctac atcgccgacc ttgccggcgg cataacgcgt tttcaggtca gctaccagcg
     8821 ccgcctcggg atggaacagg tccaggtagg tgaacaccgg gttgccatcc acgtgtccgg
     8881 gatcagtggc gtgaatgcgg ctcgggtcgg tgtacatttg catcaccttg cgctccgtct
     8941 cggctgccgt atcgcgcagg tagatggtat tgccgtacga ggtatgcatg gtccggttgt
     9001 cggttccggg caggcgcggc atccgggata aaactggctg cggttcgggg aacaacgcgc
     9061 cataggttgt gttaaaccgc cgggctacct cgcgcgccag ttccaggtgg ggcagttgat
     9121 cttcgcccac cggcacagcc tcggcccggt agagcagaat atcggcggtc tgcagcaccg
     9181 ggtaggtcag cagccccagc gatggattga gccccagcgc ctgaatctgt tctttgtagg
     9241 tgggcacgcg ttccaaccgg ctgaccggcg tcagcatgcc cagcagcagc cccagcaccg
     9301 catgttctgg aatggctgat tgcaggacca gcgttgcacg cgccgggtca atcccggctg
     9361 ccagccagtc caacaccatt tcaatcgtat tttcgcggat cctggcggcg gtttcgctgg
     9421 tcgtcagcac atgcaggtct gccaccagga agaagcactc ccgcgtgtct tgcagcgcgg
     9481 cccaggtttg cagcgtgcca accaggtggc cgatatgtcg cggcccggtg gggcgatgcc
     9541 ccgtcaatat acgtgttctt gcagtcatct gacacctccg tctgtggtca aaacaaaacc
     9601 cggcgttcat ccagaaggac gaacgccggg ttcgtggtgc cacctccgtt aggctgccat
     9661 ttgaccttaa caaaaaatcc ccggtgataa gcacacaggg ggtaacaggc cagcctcact
     9721 ctttgcgggt acggcagcaa gctgcgatac cctttgccct gataacggtg gcagctccgg
     9781 cgcaggctac tggcagatgg tgcgtttgcc tggcaactcc aaggtccatt cggcgccggg
     9841 gtgcgggcag gttctcaccg tctcctgctc tctggaccgt ccgttcagcg cgtactcgtc
     9901 ctcttcaacg tctttggata ttgaatta
//
LOCUS       DAOPVS010000068         9921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_6869_length_9921_cov_61.365802, whole genome shotgun sequence.
ACCESSION   DAOPVS010000068 DAOPVS010000000
VERSION     DAOPVS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6869_length_9921_cov_61.365802"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PKL11_06860"
     CDS             complement(<1..914)
                     /locus_tag="PKL11_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNZ13361.1"
                     /translation="MDIRIGSHTLGLTHPTYFIADIAANHDGSLERAKLLIRLAKEAG
                     ADAAKFQNFRAAQIVSDYGFRSLGGQQSHQSKWKKSVFEVYQGASVPFEWTPELKAVC
                     DEVGIDYFSSPYDFEAVDHLDSYVPAHKIGSGEIDWLEELEYIARKGKPVLLATGAAY
                     LGEVQRAVHTILAINPQLVLMQCNTNYTGSIENFNHVHLNVLKTYALMFPELVLGLSD
                     HTPGHAAVLGAVALGARVVEKHFTDDTSREGPDHGFALDPRTWREMVDRTRELERALG
                     SADKQVAENERETVIIQRRCLRAARDLRA"
     gene            complement(985..1914)
                     /locus_tag="PKL11_06865"
     CDS             complement(985..1914)
                     /locus_tag="PKL11_06865"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ13362.1"
                     /translation="MARVLITGASGLLGIHLALRAAGEHTVTGVVNTRGLPGAPFRVA
                     AADLSGTDAVTRLLDETRPEILFHCAALTHLDAVEAEPERAEWLNAGLPGLLAAECAQ
                     RGTRLVHISTDAVFDGARPYIPGDGYTEADLPNPLSVYARTKLAGEQAVLSANPQAAV
                     ARVNFYGWSLTGRRSLGEFFFNNLSAGQRINGFTDVMFCPLLATDLADILLEMARRGL
                     NGVYHTVSSEAVSKYEFGCRLARHFGLDESLITPIRVSESGLKAARSPNLALNTGKLA
                     AALGRALPEVDAGLARFAQEYHSGLPNRLQKIC"
     gene            complement(1916..2932)
                     /gene="pseB"
                     /locus_tag="PKL11_06870"
     CDS             complement(1916..2932)
                     /gene="pseB"
                     /locus_tag="PKL11_06870"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNZ13363.1"
                     /translation="MNWNDKVVLVTGGTGSFGKKFIRILLEQYNPAKVIVFSRDELKQ
                     HEMRVAGYNAPNLRYFIGDIRDRDRLHRAFNGVDIVVHAAALKQVPACEYNPMEAIKT
                     NILGSSNVIDAALDAGVQRVLALSTDKAVNPVNLYGATKLAAEKLFVQSNAYAGGRAT
                     RYACVRYGNVVGSRGSVVPVFLKQRETGRLTITDERMTRFWISLEQGVRFVIRCIEQM
                     QGGEVFVPKIPSTTIVDLARAVAPQAKIDVIGIRPGEKLHEVLISDDEARTTLELDDM
                     FVVQPAEALWFGRAWEKQGRPLPDGFRYASNTNPEWLNIDQIRQMIAPIEAEYQSGKL
                     ANGE"
     gene            complement(2952..3806)
                     /locus_tag="PKL11_06875"
     CDS             complement(2952..3806)
                     /locus_tag="PKL11_06875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13364.1"
                     /translation="MTNPDVKRQVADFYDQVGWQTEADGLYQNARYEDLRPVAREYIH
                     RCHMRIKRYIKPQGRLLLDAGSGPVQYPEYRTYSEGYAHRVCADISIVALKEARQRLG
                     EHGLYVVCDVARLPFAPEVFDGVVSLHTLHHLPVEEQPRAYRELHRVLAPNSAAAIVN
                     GWTESALMQKTQWLVRLMEWVGRQINRRKDAKPQTAPAGSSKPAAPTGTFVRKLNAEG
                     LRRELGGEMDLEILVWRSVSVRWLRAVIHRVLGGKLWLRLLYALEERWPHWFGEKGQY
                     PLVVIRKQ"
     gene            complement(3787..5406)
                     /locus_tag="PKL11_06880"
     CDS             complement(3787..5406)
                     /locus_tag="PKL11_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13365.1"
                     /translation="MTPSGKDAVKRVLGQIPFAAELYWLVRQNGRPISTRFSLKHLQT
                     ALPEVVQQAAALRAQAPAGRKVFVFATLHYWIEHAAMLALGLAAQGHEVTLGFLPYAE
                     WQKPINRFDLRRQNAYARRVLAPAESLIRVVSFINTRAPYTPLPANLVDAVKLVSSYD
                     SQYTLQVEDVDPESEIYKLRMLRNKDAAQSALAWLRTNRPDVVIVPNGTIQELGVVYR
                     VARSLGIPAVTYEFSEQRQRIWLAQNDEIMSQNTDTLWEACQSEPLSEPQIEKMRSLM
                     MARQRGMLWQNFARLWQGAPAQGGEQARQALGLDDRPVVLLATNVLGDSLTLGRQVFS
                     HNMAEWITRTVQYFIERPDIQLVIRVHPGETLTHGQSMVDVIHSVLPKLPEHIRVIKP
                     DEKINTYDLVDVADLGLVYTTTVGMEMAMNGVPVIVAGKTHYRNRGFTHDPDSWVAYF
                     KLLGHLLENPAEQRLTREQVQRAWQYAYAFFFHYPRPFPWHLVKLWDDVKTHPLNEVF
                     GPEGQAFYGETFNALLGEALDWKATHDEPGR"
     gene            complement(5403..6671)
                     /locus_tag="PKL11_06885"
     CDS             complement(5403..6671)
                     /locus_tag="PKL11_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13366.1"
                     /translation="MSDLAIRVENLGKRYRLGSTATAYTTLRETLSEWAGSPLRRRLG
                     KDEEETLWALRGVSFDVKQGQVLGVIGRNGAGKSTLLKILSRVTDPSEGWAEIHGRVG
                     SLLEVGTGFHPELTGRENIFLNGAILGMHRTEIARKFDEMVEFAEVEKFIDTPVKRYS
                     SGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQRKCLGKMSDVASQGRTVLFVSHNM
                     SAILRLTEETLVIDQGRLAMRAPTTEAVDYYLSNGFARTGERTWSAAEVPAAAAPFQP
                     IALRLLDRQGRIVDTLRSTEPASIEIEYKLAAPVQGLRVGLYLLSARGETVFTAFDTD
                     DPDVYEQQGTRAAGHYISRCALPADLLNEGRFILGINASAYRIKRYFQDEQALTFSVD
                     AAGAPGMQWPEPRLGAVRPRLEWQIEVLNQ"
     gene            complement(6675..>7484)
                     /locus_tag="PKL11_06890"
     CDS             complement(6675..>7484)
                     /locus_tag="PKL11_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13367.1"
                     /translation="LRPIKGWAPLNWRDLMDFRELTYFLTWRNIKVRYKQTLLGASWA
                     VLEPFLTMVVFSIFFGNLAQVGSDGVPYPLFSFAGLLPWGLFSKALNDASRSLVNNRH
                     MITKIYFPRLILPLSSILSGLVDFAIAFVVLLGMMLYYKTWPTAAAWWLPLLLLLALV
                     TALGVGLWLAALNVMYRDVGYIIPFLTQFWMFITPVVYPASMIPEKWRLLYGINPMTG
                     VVEGFRWALLGIQPQPTFWPMLGISAGVALLLLVSGLFYFRRMERLFADMV"
     assembly_gap    7485..7584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7585..8352)
                     /locus_tag="PKL11_06895"
     CDS             complement(<7585..8352)
                     /locus_tag="PKL11_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNZ13368.1"
                     /translation="MTSASEFWKNRRVCVTGGAGFLGRHVQARLRQGGATEIFVPTIE
                     QYDLVKGVDIRRMLDDARPDVIIHLAAHVGGIGANREKPAEFFYDNLMMGVELMHAAW
                     QKGVEKFVAIGTVCAYPKFTPVPFKEDDLWIGYPEETNAPYGLAKKMLLVQAQAYRQQ
                     YGYNAIFLLPVNLYGPGDNFDPRSSHVIPALIRKMLEAQDRGEHEVTLWGDGSPTREF
                     LYVEDAAEGIVLAAEKYNKSDPVNLGSGNEISIKDLAE"
     gene            complement(8349..8849)
                     /locus_tag="PKL11_06900"
     CDS             complement(8349..8849)
                     /locus_tag="PKL11_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNZ13369.1"
                     /translation="MSSTTSPDSHRDLIILEEIEQQPDSTQATLAAKLGVAIGTINWH
                     LKRLVDKGYVKVRRIERRKFRYLITPEGVALRARLTVDYIQTSFGLYRLIRQRTQEAL
                     AQAHAGGAEAVRVIGEGEVAEVCRLTCLEQGLPLLDAEAPANGRTALLRIKELKIFLE
                     WEEKRS"
     gene            9056..9889
                     /locus_tag="PKL11_06905"
     CDS             9056..9889
                     /locus_tag="PKL11_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ13370.1"
                     /translation="MSANFILDVNEANFEYEVISFSQNTPVVAAFWAAWRQPAQELSA
                     LLERLAREAQGAFRLARVDADRNPNLTVRYGVRSLPTVKAFTQGQVVAEFVGLQPEGR
                     VRDFLSSITPPSPFNLALERADGFYHLHQYDQAEKAYRRLLELQPGTPAALLGLARTL
                     LRRGTAPEALTILKDFPDSRQLVQAELLLPLAERLMDLQAGRLPAEADLDALFTRALQ
                     MAQRGNIYAALDGLIELLRQDKRYGSGRARQAALGLIELLGDDDPATRSYRNELATAL
                     F"
BASE COUNT         1921 a         3179 c         2837 g         1884 t
ORIGIN      
        1 gcccgcagat cacgcgccgc gcgcaaacag cgccgctgga tgatgaccgt ctcgcgttca
       61 ttttcggcca cctgtttgtc tgccgagccc agggcccgtt ccaactcgcg ggtgcggtcc
      121 accatctcgc gccaggtgcg cgggtccagc gcaaacccat gatccggccc ctcgcgggag
      181 gtgtcatcgg tgaagtgctt ttccaccacg cgggcgccca gcgccaccgc acccagcacc
      241 gccgcatgcc ccggtgtatg gtccgacagc cccagcacca gctcggggaa catcagcgca
      301 taggttttta acacattcaa atgcacatgg ttgaagttct caatgctgcc ggtgtaattc
      361 gtgttgcact gcatcaaaac caactgcggg ttgattgcca gaatggtatg cacagcgcgc
      421 tgcacctcgc ccaggtatgc cgcaccggta gccagcagca ccggcttgcc cttgcgggca
      481 atatattcca gctcttccag ccagtcaatt tcgcccgagc cgatcttgtg cgccggaaca
      541 tacgaatcca ggtgatccac cgcctcaaag tcatacggcg aggagaagta atcaatgccc
      601 acctcgtcgc aaacagcttt caattcgggc gtccactcaa agggcaccga tgcaccctga
      661 taaacctcaa agaccgattt cttccatttg gactggtgcg attgctgccc gcccagcgag
      721 cgaaagccgt aatccgaaac gatctgggcc gcgcggaaat tctggaactt ggcggcatca
      781 gccccggctt ctttggctaa ccggatcagc agtttggcgc gttccaggct gccatcatgg
      841 ttggcagcaa tatccgcaat aaaataggtc ggatgggtca gaccgagcgt gtgcgaaccg
      901 atgcgaatgt ccatttcagt tcctttcaat tgttttttgg gggaggaaat tcaaccgcgc
      961 gcagcccggc gcgcctcaaa cgccttagca gatcttttgc agccggttgg gcaggccgct
     1021 gtgatattcc tgcgcaaacc gcgccagccc ggcgtcaacc tctggcaggg cgcggcccag
     1081 cgccgccgcc agcttgccgg tattcagcgc caggttgggc gaacgggccg ccttcagccc
     1141 gctttcgctc acccggatgg gggtaatcag gctttcatcc agcccaaaat ggcgcgccag
     1201 gcggcagcca aactcgtatt tgctcacggc ttcgctgctc acggtgtgat acaccccgtt
     1261 caggccgcgc cgcgccatct ccagcagaat atctgccaga tcggtagcca gcagcgggca
     1321 aaacatcaca tcggtaaagc cgttgatgcg ctgcccggcg ctgagattgt tgaagaaaaa
     1381 ctcacccagg ctgcgccgcc ccgtcaggct ccagccataa aaattgaccc gcgccaccgc
     1441 cgcctgcggg ttggcagaca gcaccgcctg ctccccggcc agcttggtgc gggcatagac
     1501 cgagagcggg ttgggcaggt cggcttcggt atagccatcg ccgggaatgt aggggcgggc
     1561 gccgtcaaac accgcatcgg tggagatgtg caccaaccgc gtcccgcgct gggcgcattc
     1621 agccgccagc agccctggca ggccagcgtt cagccattcg gcgcgttcgg gttcggcttc
     1681 caccgcgtcc aggtgagtca gcgccgcaca gtgaaacagg atctccgggc gggtctcgtc
     1741 cagcaggcgc gtcaccgcat ccgtcccaga caggtctgcc gccgccacgc gaaaaggcgc
     1801 ccccggcagc ccgcgcgtat tgaccacgcc ggtaaccgta tgttcaccgg cggcgcgcag
     1861 cgccaggtga atgcccagca gcccgcttgc tccggtaatc agtacacggg ccatcttatt
     1921 cgccgtttgc caacttgcca gactgatatt cggcctcaat cggggcaatc atctggcgga
     1981 tctggtcaat attcagccat tcggggttgg tgttgctggc atagcggaag ccatcgggca
     2041 gggggcggcc ctgtttttcc caggcgcgcc caaaccagag cgcctcggca ggctgcacca
     2101 caaacatgtc gtccagttcc agcgtggtgc gggcctcatc atccgagatg agcacctcgt
     2161 gcagtttctc gcccgggcgg atgccaatca catcaatctt ggcctggggc gccacggcgc
     2221 gggccaggtc cacaatggtg gtgctgggaa tcttggggac gaagacctcg ccgccctgca
     2281 tctgttcgat gcagcggatg acgaagcgca cgccctgctc cagcgaaatc cagaagcgcg
     2341 tcatgcgctc atcggtaatc gtcaggcggc cagtctcacg ctgtttgagg aagaccggca
     2401 ccacactgcc ccggctgccc accacgttgc cgtagcgcac acaggcgtaa cgggtggccc
     2461 gcccgccggc ataggcattg gactgcacga acagcttctc ggcagccagc ttggtggcgc
     2521 catacagatt gaccgggttc accgccttat cggtgctgag cgccagcacg cgctgcacgc
     2581 ccgcatccag tgcggcgtca atcacgttgc tgctgcccag aatattggtc ttgatggctt
     2641 ccatcgggtt gtattcgcag gcgggcacct gcttcagcgc cgccgcatgg acgacaatat
     2701 ccacgccgtt gaaggcgcgg tgcaggcggt cgcggtcgcg aatatcgccg atgaaatagc
     2761 gcaggttggg cgcattgtac ccggccaccc gcatctcatg ctgtttcagt tcatcccggc
     2821 tgaacacaat caccttggcc gggttatact gctccagcaa aatgcggatg aatttcttgc
     2881 caaatgaacc ggtgccgccg gttaccagca caactttatc gttccagttc atagtcacct
     2941 ctaccttcta cttactgctt gcgaatgaca accagtggat attgcccttt ttcgccgaac
     3001 cagtgcggcc agcgttcttc cagtgcgtag agcaggcgca gccacagctt gccgcccagc
     3061 acacggtgaa tgacggcgcg cagccagcgc acgctgacgc tgcgccagac cagaatctca
     3121 aggtccattt cgccgcccaa ctcccggcgc agcccttcgg cgtttaattt gcgcacaaag
     3181 gtaccggtgg gagcggcagg tttggacgag ccagccggag ccgtttgcgg tttggcgtct
     3241 ttacggcggt tgatctgccg cccaacccat tccatcagcc gcaccagcca ctgcgttttc
     3301 tgcatcagcg ccgattctgt ccagccgttg acgatggccg cggctgaatt gggcgccagc
     3361 acccggtgca gctcgcggta ggcgcgcggc tgctcttcca ccggcaggtg gtgcagggtg
     3421 tgcagtgaga ccacgccgtc aaaaacttcc ggcgcaaagg gcagtcgggc aacatcacag
     3481 accacataca gcccgtgctc gcccagccgc tgccgggctt ctttcagcgc cacaattgaa
     3541 atatcagcac agacccggtg cgcgtagcct tcggaatagg tgcggtattc ggggtactgc
     3601 accggacccg agccggcatc cagcagcagc cgcccctgcg gtttgatgta ccgcttgatg
     3661 cgcatgtggc agcggtgaat atactcacgc gctaccgggc gcaggtcttc atagcgggca
     3721 ttttgataga gaccatcggc ctctgtctgc cagcccacct gatcgtaaaa atcggcaacc
     3781 tgccgcttaa cgtccgggtt cgtcatgcgt tgccttccag tccaacgcct cgccaagcag
     3841 ggcgttgaag gtctcgccgt agaatgcttg cccttcagga ccaaacacct cgttcaaggg
     3901 gtgcgttttt acgtcatccc acaatttgac caggtgccac gggaacgggc ggggatagtg
     3961 gaagaagaaa gcataagcat actgccaggc ccgctgaacc tgctcgcgcg tcaggcgctg
     4021 ctcggcaggg ttctccagca gatgccccag cagcttgaag taagccaccc acgagtcggg
     4081 gtcgtgcgta aagccgcggt tgcggtaatg ggttttgccc gccacaatca ccggcacgcc
     4141 gttcatcgcc atttccatgc cgaccgtagt ggtataaacc agccccaggt cagccacatc
     4201 caccaggtcg taggtgttga ttttttcatc cggcttgatt acgcggatat gctcgggcag
     4261 cttgggcagc acactgtgaa tcacatccac cattgactgc ccgtgcgtca gcgtctcacc
     4321 ggggtgcacc cggataacca gttgaatatc ggggcgctca ataaaatact gcaccgtgcg
     4381 ggtaatccac tcagccatat tgtggctgaa gacctggcgt cccagcgtca ggctgtcgcc
     4441 cagcacattg gtcgccagca gcaccaccgg gcggtcatcc agccccagcg cctggcgagc
     4501 ctgctcgccg ccctgggcgg gcgcgccctg ccacagccgg gcgaaattct gccacagcat
     4561 cccccgctgg cgggccatca tcaaagagcg cattttctca atttgcggct cgctcaacgg
     4621 ttcgctctgg caggcctccc acagcgtatc ggtgttctgg ctcataattt catcattttg
     4681 cgccagccag atgcgctgcc gctgctcgct gaattcatag gtcactgcgg gaatgcccag
     4741 cgaacgcgcc acgcgataca ccacgcccaa ctcctggatg gtgccgttgg gcacaatcac
     4801 cacatcgggg cggttggtgc gcagccaggc cagcgccgat tgggcggcgt ctttgttgcg
     4861 cagcatgcgc agcttgtaaa tttcactctc ggggtccaca tcttccacct gcagggtgta
     4921 ctgcgaatcg tagctggaca ccagcttgac ggcatccacc aggttggcgg gtaggggcgt
     4981 gtacggtgcc cgagtgttga tgaaggaaac caccctgatc aacgactcgg ccggcgccag
     5041 cacccggcgg gcgtaagcat tctgccgccg caaatcaaac cggttgatcg gcttctgcca
     5101 ttcagcatag ggcagaaaac ccagcgtcac ctcgtgcccc tgggcagcca gccccagcgc
     5161 cagcatggcg gcatgctcaa tccagtagtg cagggtggca aaaacaaaaa ccttgcgtcc
     5221 agcgggggcc tgggcgcgca gcgccgcagc ctgctgaaca acctcgggca gagcggtttg
     5281 cagatgtttg agcgagaagc gggttgaaat ggggcgtccg ttttgccgga ccagccagta
     5341 gagctcggcg gcaaagggga tttgccccag cactcgtttg acggcgtctt tgcctgatgg
     5401 tgtcattggt tcaaaacctc aatctgccat tctaaacgcg ggcggacagc ccccagccgc
     5461 ggttcgggcc actgcatgcc cggcgcgccg gcggcatcca ccgaaaatgt gagcgcctgc
     5521 tcatcttgaa aatatcgctt gatgcggtag gcgctggcgt tgatgcccag gatgaaacgc
     5581 ccctcgttca gcaggtcggc gggcagcgca caccggctga tgtaatgccc ggccgcgcgg
     5641 gtgccttgct gctcataaac atcggggtca tccgtatcaa aggcggtaaa aacagtctcg
     5701 ccgcgggcgc tgagcaaata cagccctacg cgcagtccct gaacgggggc ggcaagttta
     5761 tactcaattt caatgctggc aggctcggta gagcgcagcg tatccacaat gcgaccctgg
     5821 cggtccagca gccgcagggc aatcggttgg aagggtgccg ccgcagcggg cacctctgcg
     5881 gcgctccagg tgcgctcacc ggtgcgggca aacccgttgg acaggtagta atccaccgcc
     5941 tcggtggtgg gcgcccgcat cgccaggcgg ccctggtcaa tcaccagcgt ttcttcggtc
     6001 agccgcaaaa tagccgacat gttatggctg acgaacagca ccgtgcgccc ctggctggca
     6061 acatcgctca tcttgcccag gcacttgcgc tggaactcgg catcgccgac tgccagcacc
     6121 tcgtccacca ccagaatctc gggctccagg tgggctgcca cggcaaaagc cagccgcagg
     6181 tacatgccgc tggaataccg cttgaccggg gtatcaataa atttctctac ctcggcaaat
     6241 tccaccattt catcgaattt gcgggcaatt tcagtccggt gcatccccag gatggcgccg
     6301 ttcaggaaga tattttcccg tccggtcagc tccgggtgaa agcctgtgcc aacttccagc
     6361 agcgagccta cccgcccgtg aatctcagcc cagccttcag atgggtctgt aacccggctg
     6421 aggatcttga gcagcgtgct tttacccgca ccgttgcgcc cgatcacgcc cagcacctgc
     6481 ccctgcttaa cgtcaaacga gacgccgcgc agcgcccaca gcgtttcttc ttcatccttg
     6541 cccaagcgcc gccgcagtgg tgaaccagcc cattcgctca gggtctcgcg cagggtggta
     6601 taggccgttg ccgtgctgcc cagccggtag cgcttgccca gattttcaac ccgaatcgcc
     6661 agatcactca ttggttacac catgtcggca aacagccgct ccatgcgccg gaagtagaac
     6721 agcccgctga ccagcagcag cagcgccacc cccgccgaaa tgcccagcat gggccagaag
     6781 gttggctgcg gttgaatgcc cagcagcgcc cagcgaaagc cttccaccac acccgtcatc
     6841 gggttaatgc cgtacagcag ccgccacttc tcaggaatca tgctcgccgg atagactacc
     6901 ggcgtaatga acatccagaa ctgcgtcagg aaggggatga tatagcccac atcccggtac
     6961 atcacattca gcgcggccag ccacaacccc acccccagcg cagtgaccag cgccagcagc
     7021 agcagcagcg gcagccacca ggccgcggcg gtgggccagg tcttgtagta cagcatcatg
     7081 cccagcagca ccacaaaggc aattgcaaaa tccaccagcc cgctgagtat ggacgacagc
     7141 ggcagaatca gccgcggaaa atagatcttg gtaatcatgt gccggttgtt caccagcgag
     7201 cggctggcgt cgttgagcgc cttggagaac agcccccagg gcagcagccc ggcaaacgag
     7261 aagagcgggt acggcacgcc gtctgaccca acctgcgcca gattgccgaa gaagatggag
     7321 aaaaccacca tcgtcaggaa tggttccaaa accgcccagg atgcgcccag cagcgtttgc
     7381 ttatagcgca ccttgatgtt tcgccaggtg agaaaatacg tcagctcgcg aaaatccatc
     7441 aggtcgcgcc agttcagcgg cgcccagcct ttgatggggc gcagnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnctcggc caggtctttg atgctgattt cattgccaga
     7621 acccaggttc accgggtccg atttgttgta tttctcggct gccagcacaa tgccctcggc
     7681 agcatcttcc acatacagaa actcgcgggt gggcgagccg tcgccccaca gcgtcacctc
     7741 gtgttcgccg cggtcctgcg cttcaagcat cttgcggatc agcgccggga tgacgtgcga
     7801 cgagcgcggg tcaaaattat cgccggggcc gtacagattg acgggcagca ggaagatggc
     7861 attatagccg tactgctgac gataggcctg cgcctgcacc agcagcatct tttttgccaa
     7921 cccataaggg gcgttggtct cttcggggta gccgatccac agatcgtctt ctttgaatgg
     7981 caccggggtg aacttggggt aagcgcaaac cgtgccgatg gccacaaatt tttccacgcc
     8041 cttctgccag gccgcgtgca tcagctccac gcccatcatc aggttgtcgt agaaaaattc
     8101 ggcgggtttt tcgcggttgg cgccaatgcc gcccacatgc gccgccaggt gaataatcac
     8161 atccgggcgg gcatcatcca gcatccggcg gatatccaca cccttcacca ggtcatactg
     8221 ttcaatggtt gggacgaaaa tttcggtggc gccgccctgg cgcagccggg cctgcacatg
     8281 ccggcccaga aaacccgccc cgcccgtcac gcaaacgcgt cgattcttcc agaattcaga
     8341 tgcagaggtc atgaccgttt ttcctcccac tccaaaaaga ttttaagttc tttgattcgc
     8401 agcagtgcgg tacgcccatt ggcaggggcc tcggcatcca gcagcggcag gccctgctcc
     8461 aggcaggtca accggcagac ttcggccact tcgccttcgc caatcacccg caccgcctcg
     8521 gcgccgccgg catgggcctg cgccagcgcc tcctgtgtgc gctgccggat cagccggtac
     8581 agcccaaacg aggtctgaat gtaatccact gtcagccggg cgcgcagggc caccccttcg
     8641 ggcgtaatca gatagcgaaa tttgcgccgc tcaatgcgcc gcactttgac gtatccctta
     8701 tcaaccaggc gcttcaaatg ccagttgatg gtcccaatgg ctactcccag tttggcagcc
     8761 agcgtggcct gggttgaatc gggctgctgc tcgatctctt cgaggataat caggtcgcga
     8821 tggctgtcgg gggaggtggt actgctcata aaggttccaa tattcaacgg ctgaattata
     8881 cccgccgccg ctgtttcggt caagggaggt tttgcaggcg gcggctcccc ttgcccggcg
     8941 cagttgtgtg gtgcctttcc cggttcggta aacatttttg agtcccaacc cccggcgctg
     9001 gtgattgtac tttatcccca tttttcggta caataagcat gatggagact ctggcatgag
     9061 cgcaaacttt attctggatg ttaacgaagc caactttgaa tacgaagtga tcagcttctc
     9121 ccagaatacg cccgtggtgg cggcgttttg ggctgcctgg cgtcagcccg cccaggagct
     9181 ttcggcgctg ctcgagcgcc tggcgcgtga ggcccagggc gccttccggc tggcgcgggt
     9241 ggatgccgac cgcaacccca acctgacggt gcgctacggc gtccgcagcc tgcctaccgt
     9301 caaagccttc acccaggggc aggtggttgc cgagtttgtg ggcctgcagc ccgaaggccg
     9361 agtccgcgac tttttgagca gcatcacgcc gcccagcccc ttcaacctgg cgctggaacg
     9421 cgccgatggc ttctatcacc tgcaccagta tgaccaggcc gagaaggctt accgccgttt
     9481 gctggagctg cagcccggca cccccgccgc cctgctggga ctggcgcgca ccctgctgcg
     9541 ccgcggcacc gcccccgagg cgcttaccat cctgaaagat tttcccgaca gccgccaact
     9601 ggttcaggcc gaactgctgc tgccgttggc tgaacggctg atggatttac aggccgggcg
     9661 gctgcccgcc gaggccgacc tggatgcgct cttcacccgc gccctgcaga tggcccagcg
     9721 cggcaatatc tacgccgctc tcgatggatt gattgaactg ctgcgccagg acaagcgcta
     9781 tggcagcggc cgcgcccgac aggccgctct ggggttaatt gaactgctgg gcgatgacga
     9841 cccggccacc cgcagctatc gcaatgaact ggctacggcg ttgttctaac actatttcgg
     9901 aggtatccaa atgaaatttt c
//
LOCUS       DAOPVS010000069         9773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7007_length_9773_cov_57.962132, whole genome shotgun sequence.
ACCESSION   DAOPVS010000069 DAOPVS010000000
VERSION     DAOPVS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7007_length_9773_cov_57.962132"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3437
                     /locus_tag="PKL11_06910"
     CDS             <1..3437
                     /locus_tag="PKL11_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNZ13371.1"
                     /translation="NMNGAIIHGDGNGGCWQGITFLPGSGGNILNSTIQDATVGIAIQ
                     SASPQILGNTISNLSGDAQTPSSIATDAIGVWVYGVGALPVVNENTIQHVNGGFGLNG
                     QIVSPGGNAYGVLITSGASPTVQNNDILNIYGGNGGVGQGGLAGLPGAPGTEFSPNGG
                     NGTDGGPGTGGGSGGEAVGIALTSSSALVSGNFISDVRGGAGGFGGAGGPGGPGGNGY
                     GATDIDVGLTGGSGGSGGGGGAGGMGGGGGVAAGIRSLLGVPLTAYDNYIQIVLGGAA
                     GDGGPGGPGGDGGLGGNGGNGAPGTPPGAGGNGGSGGAGGAGGSGGLGGQADGIHLHD
                     SSLPQPLDRNSTYTILAGNGGSGGSGGPGGLNGGLGGNGGSSPLHSGGAGGAGGNGGA
                     GGAAGAAGNGGVAAALRLENFTGVYVVTNHLGVDVAAGEGGVGGNGAPGGMGGPGGMG
                     GFGTVQQAPGGNGGNGGSGGAAGAGGGAGDAHLAFVQQTTAVLTNNTLVEVYTALTGG
                     ASGQVGPGGMGGPGGMGVPPGATGANGSPGAASIPGAGGSAYGALIFTGGVGQVHNTI
                     LFNILSRTNKTALAQLAGGILSTEDYNNLYNWGVNYGGVPVGAHNLLTDPLFVDPNNN
                     NFHLTTPNAPGIDSADPSAPQCPTLDYDTINRPIDGNDDGTAVVDMGAFEDGSYLQFD
                     WASISTNEDEIILQVTVSRLGNPALTASVDYATGGGDAAAGQDYDSASGTLTWTAGDA
                     SGKTINITIHEDTTDELDETFNVTLSNPSSAFLGTPAVSTITILDDDAPAVCTIQFAQ
                     AASSAGENAGTTTIAATRTGGGCSAAGSADYAVTGGTATPGSDFTGGSGTLNWAAGET
                     GTKTFTLTIADDAVDELDETVIFDLSNLTGATAGTPIQTTLTIGDNDTCQVAFSAAAY
                     AVLEDDVSATVTVSLDQACAAPAQVDYATSNGSAQSGSDYTATAGTLSWSAGDPAPQT
                     FFVDILQDAETEGDETINLLLSNPSGANLGDPSSAVLTIIDDENPSTCLVRFTASEYA
                     VDETTSSATITVMVEPAAQCTGGLSVDYATSNGTATAGADYTTASGTLTWAAGNTNSQ
                     TFSIPLLDDSLVEGDETVILTLSDPVGAVLGAPADSVLIIRDNDVAHFICLPVILK"
     gene            complement(3492..4349)
                     /locus_tag="PKL11_06915"
     CDS             complement(3492..4349)
                     /locus_tag="PKL11_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNZ13372.1"
                     /translation="MLYLLFVSLLWAFSFGLIKGNLAGVDPVFVAFARILIALLVFLP
                     FARLRGVTRRQGLQLLGIGAVQYGLMYIFYNAAFAYLQAYEAALFTIFTPLYIVLIND
                     GLTRRVHFFALWVAALAVIGTGIISYNGGVSTAQWLGFGLMQLSNLCFALGQVAYRRV
                     MTGSAASDASIYGLVYLGGALATGLAAALFTPWQALMLTTRQIWTLVYLGAVASGLGF
                     FLWNMGARRVNVGALAIFNNLKVPLSVAVSLLVFGETVRLPSLILGGLIVLGALVWNE
                     LHQRQTAKA"
     gene            4475..4675
                     /locus_tag="PKL11_06920"
                     /pseudo
     CDS             4475..4675
                     /locus_tag="PKL11_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            4672..5664
                     /gene="trpS"
                     /locus_tag="PKL11_06925"
     CDS             4672..5664
                     /gene="trpS"
                     /locus_tag="PKL11_06925"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ13373.1"
                     /translation="MMAKKGRVFSGARPTGRQHLGNYLGAMRNYVALQADYECIYCIV
                     DLHALTTMETTRDLRQNTYEMALDWLAVGIRPDESIMFVQSHVPQVTELHTILSMVTP
                     LGKLTDLPTFKEKVRQQPNNVNLGLVGYPVLMAADIVLYKAGLVPVGVDQAPHIEFAR
                     ETVRSFNYRYGAAVLIEPDMKVTEVPKVVGIDGVQKMSKSLDNHIELAATPEETSKRV
                     MSMVTDPQRQRRTDPGRPEVCNVFALHKVFSPADEVAMIDTECRRAGIGCVDCKKRFA
                     ANLNAHLEPFRTRRAELDCNPAAVWDILHEGAGRARAIAEETMREVRTAIGLPE"
     gene            5671..6108
                     /gene="dtd"
                     /locus_tag="PKL11_06930"
     CDS             5671..6108
                     /gene="dtd"
                     /locus_tag="PKL11_06930"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNZ13374.1"
                     /translation="MKAVIQRVTRGSVTIEGRRVAEIDLGLVILLGVGPGDDEEKARL
                     LARKIAMLRIFGDENGKMNLSVLDVNGAAIVVSQFTLYADTRKGHRPAFTDAAPPEIA
                     RPLCDRFTDLLREQGVPVQTGEFGAHMQVEIQNDGPVTISLEY"
     gene            complement(6146..6898)
                     /locus_tag="PKL11_06935"
     CDS             complement(6146..6898)
                     /locus_tag="PKL11_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13375.1"
                     /translation="MSDNLPRQELYSRSKWLISGLGFAPQAALPALPEPPGAFITAPI
                     SLSARTPAESRACLNYPGGFLLHTGLPNPGLRAVLKHYAARWERAELPVWPHLIPATP
                     DEADRMGRLLEGRENVTALVISPPPHLKDREQMDLLAAARGELPIIAEMPLSGISAAW
                     VETLAGLGISAVLLGAPRGCLPNGEGKLVSGRLYGPGLLPQMLEVVRAVKRTGLPVIA
                     GAGVFSKSDGLAVLAAGAWAVTMDAALWRPAP"
     gene            complement(6895..7581)
                     /locus_tag="PKL11_06940"
     CDS             complement(6895..7581)
                     /locus_tag="PKL11_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13376.1"
                     /translation="MAEFESVLTEVTLSTGGMGGILTCPPRLQVQPGQYLLGYAPELN
                     EPRTTTFFPVEIQPGALRLAAPLPQTWAAGMPLLVRGPLGRGFTLPAQARRVALAALN
                     GSAERLTPLMTAALAQGAAVVLFSGSPPASVSDEVEILPLEQLPEAPAWADYLALDLM
                     PAQAGNWRKLLAIRPTQPCPCSGEALIITDMPCGGLAECGVCAVETRLGWRLACKDGP
                     VFPLSELEAR"
     gene            7633..8037
                     /locus_tag="PKL11_06945"
     CDS             7633..8037
                     /locus_tag="PKL11_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNZ13377.1"
                     /translation="MPPNWLDNLKTRAHLLKRDALALYFAYRDPRTPWYARLFAAYVV
                     ARTFSPIDLIPDFIPVLGYLDDLILTPLGIALALKMIPAQVMMAARQQADASLQFKKP
                     INGLYIALVILIWLLLALVVGLVVWNLAHTGA"
     gene            8095..9021
                     /locus_tag="PKL11_06950"
     CDS             8095..9021
                     /locus_tag="PKL11_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNZ13378.1"
                     /translation="MLRYKVILNPAAGRGTARAQLPFVRQALEGYGLSIDVTETARPG
                     HALDLARQAVADNFDVIVAGGGDGTVNETINGMMQAKQQGLGRAALAVIPLGRGNDFA
                     ASMGVGADWQRACLAVGENHRRWIDIGRSVGEFYPEGRYFGNGVGIGFDAVVGFQAAK
                     MAHISDSSYVVAAIKTIFLYFKAPEVQLVVDGQTSTLPCLMINIMNGRRLGGKFWMTP
                     NAKPDDGIMDLCIARQVSRLEILKIMPLYQSGTQHTHPAIRTMHGKRVTITALKGTLP
                     AHADGETLCTEGHHLEIEILPHQLELIIPQDA"
     gene            9018..9710
                     /locus_tag="PKL11_06955"
     CDS             9018..9710
                     /locus_tag="PKL11_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ13379.1"
                     /translation="MKLSVHVVNRTIKFVIRMMFDMQDAELARVPQQGPLILAVNHVN
                     SMDAPMGLSHLYPRPVAAIAKVETWDNPLFRFLFNVWDGIPIRRGEADLSAIRLAQEA
                     LAAGKIVAVAPEGTRSRHGCLQEGHPGIVLLAVRSGAPILPVVYYGHADYKEQLLHFR
                     RPRVQIVVGQPFYLDAGGKALSRDVRDQMTAEIMYQMAALLPPHFRGHYADMKQATQA
                     YLRFGPQAALQF"
BASE COUNT         1937 a         3055 c         2884 g         1897 t
ORIGIN      
        1 taaacatgaa cggcgccatt atccatggcg atggcaatgg cggctgctgg cagggcatca
       61 ccttcctgcc cggcagcggt ggcaatatcc tcaacagcac cattcaagat gccaccgttg
      121 ggatcgccat ccagtccgcc tcgccccaaa ttctgggcaa caccatctcc aatttgtcgg
      181 gtgatgccca gacccccagt tcaatcgcta ccgatgccat tggcgtatgg gtttatggcg
      241 ttggggcgct gccagtggtc aatgagaata ccatccagca tgtgaatggc gggtttggtc
      301 tgaacgggca aatcgtcagc ccgggcggaa atgcctacgg cgttctgatc acttcgggcg
      361 cctcgccaac cgtccagaat aatgacattc tcaatattta cggcggtaat ggcggggttg
      421 ggcagggtgg cctggctggc ttgcccggtg cgcccggcac agagtttagc cccaatggtg
      481 gcaatggcac ggatggcgga cccggcactg gcggcggtag cggtggcgag gctgtgggca
      541 ttgccctgac ctcgtccagc gcgctggttt cgggtaactt tatttcagat gtgcgtggcg
      601 gagcaggggg atttggcggc gctggcggcc ccggtgggcc cggcggcaat ggctatggcg
      661 ccacagatat tgatgtcggc ctgacgggcg gcagcggcgg cagcggcggt ggtggaggcg
      721 cgggcggcat gggcggcggt gggggcgtag ccgccggaat ccgttccctg ctgggcgtcc
      781 cgctcacagc gtatgataat tacattcaaa ttgtgctggg cggcgccgcc ggagatggcg
      841 gcccgggtgg gcccggcggt gatggcggtc tgggtggcaa tgggggtaat ggcgcacccg
      901 gaaccccgcc cggcgcgggc ggtaacggcg gctcaggcgg cgcgggtggc gccggtggca
      961 gtggcggact gggcggtcag gccgatggca ttcacctgca cgattcttct ctgccccagc
     1021 cgctggatcg taacagcacc tacaccattt tggctggaaa cggcggtagt ggtggcagcg
     1081 gcggccccgg cggcctgaac ggcggcctgg gcggcaacgg cggctccagc ccgctgcatt
     1141 cgggcggcgc gggtggcgct ggcggtaatg gcggcgccgg tggtgcggct ggtgcggccg
     1201 gtaatggggg tgttgctgcc gctttgcgcc ttgaaaactt cacaggcgtc tatgttgtca
     1261 ccaatcacct cggcgtggat gtggccgctg gagaaggcgg cgttggtggc aatggggccc
     1321 ccggcggcat gggcggcccc ggggggatgg gcggattcgg gactgtccag caagctccgg
     1381 gtggcaacgg tggtaatggc ggcagtggcg gcgcagccgg ggccgggggc ggcgctggtg
     1441 atgcgcatct ggcctttgtt cagcaaacca ctgctgttct gaccaataat accctggtgg
     1501 aagtttatac ggctctgacc gggggcgctt cgggccaggt tgggcccggc ggcatgggcg
     1561 gacccggcgg catgggagtt cctcctggtg cgaccggagc caatggcagc ccgggtgcgg
     1621 cttctatccc cggcgcgggc ggctcggcct atggtgcctt gatcttcacg ggtggcgttg
     1681 ggcaggtcca caataccatt cttttcaata tactctcccg caccaacaaa accgccctgg
     1741 cacagcttgc cggaggcatt ttgtccaccg aggattataa caatctgtat aactggggtg
     1801 tcaattatgg aggcgtgccg gtcggggccc ataatctcct gaccgatccg ctctttgtgg
     1861 acccgaataa taacaacttc cacctgacca caccgaacgc tcccggcatt gattccgccg
     1921 atccatccgc tccgcagtgt ccgaccctgg attacgatac catcaaccgc ccgattgacg
     1981 ggaatgatga tggaaccgcc gtggtggaca tgggcgcgtt tgaagatggc agctacctcc
     2041 aatttgattg ggcctctatc tcaaccaacg aagacgagat tatccttcag gttacggtct
     2101 cgcgccttgg caacccagcg ctgaccgcct cggtagatta tgctaccggc ggcggtgatg
     2161 ccgcggccgg gcaggactat gacagcgcca gcggcaccct gacctggacc gcaggtgacg
     2221 cttcgggcaa gacaatcaac atcaccattc atgaagacac gacggatgag ttggatgaaa
     2281 ccttcaatgt caccctgtca aacccgagca gcgctttcct gggaacaccg gctgtcagta
     2341 ccatcaccat cctggatgat gatgccccgg cggtttgtac catccagttt gcccaggcgg
     2401 cttccagcgc tggcgagaat gctggcacaa ccaccatcgc cgccacgcgg acgggtggcg
     2461 gctgctccgc agccggctct gccgattacg ccgtgaccgg cggcacggcc accccgggtt
     2521 ctgattttac tggcggcagc ggtacgctca actgggctgc cggagagaca ggcaccaaga
     2581 ccttcaccct gacgattgcc gatgacgctg tggacgagct ggatgagacc gtcatctttg
     2641 acctgagcaa tctcaccggc gccactgctg gcactcccat tcaaaccacc ctgaccattg
     2701 gcgataatga tacctgtcag gtggccttca gcgcggccgc ttacgccgtg cttgaagatg
     2761 acgtcagtgc cactgtcacc gtgtcgctcg atcaggcctg cgctgcaccc gcccaggtgg
     2821 attatgcaac cagcaacggc tcagcccaat ccggctccga ttacaccgcc actgccggaa
     2881 cgctgtcctg gtcggcgggc gaccctgcgc cgcagacctt cttcgtggat atcctgcagg
     2941 atgccgaaac cgaaggcgat gagacgatca acctgctcct cagcaatccg tcgggcgcca
     3001 acctgggaga tcccagttcg gcagtactca ccatcattga cgacgagaac ccctccacct
     3061 gcctggtgcg ttttacggcc tctgaatatg ctgttgacga gaccacatcc agcgcaacga
     3121 tcactgtcat ggttgaaccg gctgcccagt gtaccggcgg gctcagtgtt gattatgcca
     3181 ccagcaacgg cacagccacc gccggggcag actataccac cgccagcggt acgctcacct
     3241 gggcggcggg caataccaac tcgcaaacgt tcagcatccc gcttctggat gacagcctgg
     3301 tggaagggga tgagaccgtt atcctgacgc tcagcgatcc agtgggggcg gtgctgggcg
     3361 cccccgcaga ctcggtattg atcatccggg ataacgatgt agcccacttc atttgtttgc
     3421 cggtgattct caagtaaggc cgcctggctt agcccgaggc ggcctgtcca ccgcctcggg
     3481 ctgatggctt ttcaggcttt ggctgtctgg cgctggtgca gttcattcca gaccaacgcc
     3541 cccagcacaa tcaaccctcc cagaatcagg ctgggcaggc gcaccgtttc gccaaacacc
     3601 agcagtgaaa ccgcaaccga gagcggcact ttgaggttgt tgaagattgc cagtgcgcca
     3661 acgttaaccc gccgcgcccc catattccac aggaagaaac ccagcccgga ggctaccgcg
     3721 cccaggtaaa ccagtgtcca gatctgcctg gtggtcagca tcagcgcctg ccagggcgtg
     3781 aacagagccg ccgccaaccc ggttgccagc gccccaccca ggtacaccag tccataaatg
     3841 gacgcgtctg aggctgcgct gccggtcatc acccgccggt aggctacctg ccccagcgca
     3901 aaacacaggt tagatagctg catcaagcca aaccccagcc actgcgccgt gctcacgccc
     3961 ccgttatagc tgatgattcc cgtcccaatc actgccagtg ccgccaccca cagcgcgaag
     4021 aaatgcacgc ggcgggtcag cccgtcatta atcagaacga tatacagcgg cgtaaaaatg
     4081 gtgaacaggg cggcttcgta ggcctgcaga taggcaaaag ccgcattgta gaaaatgtac
     4141 atcaatccgt actgcaccgc gccgatgccc agcagttgca gcccctgccg gcgggtgacc
     4201 ccgcgcagcc gggcaaatgg caggaaaacc agcagcgcaa tcagaatccg ggcaaaggcc
     4261 acaaacactg gatcaacccc cgccaggttg cccttgatca gtccaaacga gaaagcccac
     4321 agtaaggaaa caaacagtaa gtaaagcatg tcatcagcct cggcagtctc aaaaaacgat
     4381 tataatcagg cacaagatta acaccgtgga ggcttgcccg atgctgctca aaatcacgat
     4441 tgaatactgc gctgtttgac actacaccga gcgcgccgtc agtttgacgg ccgaactgat
     4501 gcggaatttt gagcccgaaa tcgaaaccgt taccctcatg ccctcgcagg gcggtcgttt
     4561 tgaggtgacg gtcaacggca gcctgctcta ctccaagttg gcaaccatgc gacaccccga
     4621 acccggcgaa gtgacccggg cgattaaaaa atatcttcag gatggtgaaa aatgatggct
     4681 aaaaaaggcc gtgtgttctc cggcgcccgc cccaccgggc ggcagcacct gggcaattac
     4741 ctgggcgcca tgcgcaatta tgtggccctg caggcagact atgaatgtat ctattgtatt
     4801 gtcgatttgc acgccctgac caccatggaa accacccgcg acctgcggca gaacacctat
     4861 gaaatggccc tcgactggct cgcggtgggc atccgcccgg atgaatccat catgtttgtc
     4921 cagtcgcatg tgccccaggt caccgagctg cacaccatcc tctccatggt cacccccctc
     4981 ggcaagctga ccgatctgcc caccttcaaa gaaaaagtcc gccagcagcc caacaacgtg
     5041 aacctgggcc tggtgggtta ccccgtgctg atggctgccg atattgtgct gtataaagcc
     5101 gggctggtgc ctgttggcgt ggatcaggcg ccccatattg agtttgcccg tgagacagtt
     5161 cgttcattta actaccgcta cggcgccgcc gtgctgattg aaccggacat gaaagtcacc
     5221 gaggtcccca aggttgttgg cattgacggc gttcagaaga tgagcaaaag ccttgataac
     5281 cacatcgagc tggctgccac ccccgaagag accagcaagc gcgtgatgag catggtcact
     5341 gatccccagc gccagcgccg caccgatccg gggcgccccg aagtctgcaa cgtgtttgcc
     5401 ctgcacaagg tcttctcacc ggctgatgaa gttgccatga ttgataccga gtgccgccgt
     5461 gcgggcatcg gctgcgtgga ctgcaagaaa cgctttgccg ccaacctcaa cgcccatctg
     5521 gagcctttcc gcacccgccg tgccgagctg gattgcaatc cggctgctgt ttgggatatt
     5581 ttgcatgagg gcgccggccg cgcccgcgcc attgccgaag agacaatgcg tgaagttcgc
     5641 actgccattg gtctgcccga atagctgccc atgaaagctg tgatccagcg cgtcacccgc
     5701 ggcagcgtga ccattgaagg tcgccgcgtg gccgaaattg acctgggcct ggtgattcta
     5761 ctgggcgttg gccccggcga tgatgaagaa aaggcccggc tgctggcgcg caaaattgcc
     5821 atgctgcgca tctttggcga tgaaaacggc aagatgaatc tgtcggtgct ggatgtgaac
     5881 ggcgcggcga ttgtcgtttc gcagttcacc ctctatgccg atacccgcaa aggccaccgc
     5941 ccggccttca ccgatgccgc cccacccgag attgcccgcc cgctctgcga tcgctttacc
     6001 gatttgcttc gagagcaggg cgtgccggtt caaacgggcg aatttggcgc tcacatgcag
     6061 gtcgaaatcc aaaacgacgg ccccgtcacg atttcattgg aatattaacc gccctctccc
     6121 tttagggaga aggccgggct gagggtcagg gcgcaggccg ccaaagcgcc gcatccatcg
     6181 tcaccgccca ggcgcccgca gccagcactg ccagcccatc tgactttgaa aacacccccg
     6241 ctcccgcaat caccggcagc ccggtgcgtt tgacggcgcg caccacctcc agcatttgtg
     6301 gcagcagccc gggtccatac agcctgccgc tcaccagctt tccttcacca ttgggcaggc
     6361 aaccgcgcgg tgcgcccaat agcaccgcac tgatccccaa ccccgccagc gtttcaaccc
     6421 aggccgcgct gatgccagac agcggcattt cggcgataat gggcagttca ccccgcgccg
     6481 ccgccagaag atccatctgc tctcggtctt tcagatgggg cggtgggctg atgaccagtg
     6541 ccgtgacatt ctcgcgtccc tccagcagcc ggcccatgcg gtccgcttca tccggcgtgg
     6601 cgggaatcaa atgcggccag actggcagtt cggcgcgctc ccagcgggcg gcataatgct
     6661 tgagcaccgc gcgcagtccg gggttgggca gcccggtgtg cagtaaaaac ccacccgggt
     6721 agttcaggca agcccggctt tctgccggtg tgcgggctga gaggctgatc ggcgcagtaa
     6781 tgaaagctcc gggcggctct ggcagcgctg gaagcgcagc ctgtggagca aaccccaacc
     6841 cggatatcag ccattttgat cggctgtaca gttcctgacg cggaaggttg tcgctcatcg
     6901 cgcctccagt tcgctcaggg gaaaaacagg gccatctttg caggccagcc gccagcccaa
     6961 gcgcgtctcc acggcgcaaa cgccgcattc tgccagcccg ccgcacggca tgtccgtgat
     7021 gatcaacgcc tcgccgctgc acgggcaggg ctgagtgggg cgaattgcca gcagttttcg
     7081 ccagtttccg gcctgggcgg gcatcagatc cagcgccaga tagtctgccc aggccggggc
     7141 ttctggaagc tgttccagcg gcagaatctc aacctcgtca gaaacgctgg caggcggact
     7201 gccagaaaac agcaccaccg cagcgccctg cgccagcgcc gccgtcatca gcggggtcag
     7261 ccgctcggcg ctgccgttga gcgcagccag cgccacccgg cgcgcctggg ccgggagtgt
     7321 aaaaccacgt cccagcggac cgcgcaccag caggggcatc ccggcagccc aggtctgggg
     7381 cagcggggcc gccagccgca gcgctccggg ttgaatctca accgggaaga acgttgtggt
     7441 tcggggttcg ttcaattcgg gcgcatagcc cagcaggtac tgcccgggct gcacttgcag
     7501 ccggggcggg caggtcagta tgccgcccat cccgccggtt gaaagggtca cttccgtcaa
     7561 tacgctctca aattcagcca tgccttgatt atagcggatt ccactccgca aaatcgtggt
     7621 attatcaagc ccatgccgcc caactggttg gacaacctaa aaacacgcgc tcacctgctc
     7681 aaacgcgatg cactggcttt gtatttcgcc tatcgcgacc cgcgcacccc ctggtatgcc
     7741 cgcctgtttg ctgcctatgt ggttgcccga acgttcagcc ccattgacct gatccccgat
     7801 tttatccctg ttttgggcta tctcgacgac ttgatcctca ccccgctggg catagccctg
     7861 gctctaaaga tgatccctgc ccaggtcatg atggctgcgc gccagcaagc cgatgcctct
     7921 ctgcaattta aaaaaccgat caatgggctt tatatcgctc tggttatcct gatctggttg
     7981 ttattggcgc ttgttgttgg cctggtggtc tggaatctgg cccatacagg tgcttgacaa
     8041 accctttatc ctgattacaa tcaaggtatt caaaaccaaa ggaatcaata cgcaatgctg
     8101 cgatacaaag taattctcaa tccggcggct ggccgtggaa ctgcccgagc acagctccct
     8161 tttgtccgcc aggcgctgga aggttatggc ttatcgatag atgttaccga aacggctcgt
     8221 cctggtcatg cgctcgatct ggcgcgtcag gccgtagctg ataactttga cgtcattgtc
     8281 gctggtgggg gcgatggcac ggttaacgaa accattaatg gcatgatgca ggccaaacag
     8341 caaggattgg gccgtgcggc gctggcagtc atccccctgg ggcgtggcaa cgactttgcc
     8401 gccagcatgg gcgtgggcgc cgattggcag cgtgcctgcc tggcagtggg cgaaaaccac
     8461 cgccgctgga ttgatattgg ccgttctgtc ggcgagtttt accctgaagg acgttatttc
     8521 ggcaacggcg tgggcattgg ctttgacgcc gtggtcggct tccaggctgc caaaatggca
     8581 catatctctg attcttccta tgtggttgca gccatcaaga ccatcttcct atatttcaaa
     8641 gcccccgaag tccagcttgt cgtggatgga cagacctcaa cgctgccctg cttaatgatc
     8701 aacatcatga acggccggcg cctgggcggc aaattctgga tgacgcccaa tgccaagccc
     8761 gatgacggca ttatggattt gtgcattgcc cggcaggtca gccggttgga aattcttaaa
     8821 atcatgccgc tgtatcaaag cggcacacag cacacgcacc ccgccatccg caccatgcat
     8881 ggcaaacgcg tgaccattac cgccctgaag gggacgctac ccgcccatgc cgacggtgaa
     8941 accttgtgca ccgaaggcca tcacctggaa attgagatcc tgccccacca gcttgagctt
     9001 attattccgc aggacgcatg aaattatcag tacacgtggt caatcgaaca attaaattcg
     9061 tcatccgcat gatgtttgat atgcaggatg ccgaactggc gcgggtgccc cagcagggcc
     9121 cgttgatttt ggccgtgaat cacgtcaatt caatggatgc ccccatgggc ctctcgcatc
     9181 tttatccccg ccccgtagct gccattgcca aggtcgaaac ctgggataat cccctgtttc
     9241 gcttcctgtt caacgtctgg gatggcatcc ccatccgccg cggcgaagcc gacctgtcgg
     9301 cgatccgcct ggcgcaggag gcgctcgccg ccggcaagat tgtggccgtg gcccccgaag
     9361 gcacccgcag ccggcatggc tgtctgcagg aagggcaccc cggcattgtg ctgctggcgg
     9421 tgcgcagcgg tgcgcccatc ctgccggtgg tctattacgg ccatgcagac tacaaagaac
     9481 aactcctgca cttccgccgc ccccgggttc aaattgttgt tgggcagcca ttttacctgg
     9541 atgcaggcgg caaggccctc tcccgtgatg tgcgcgatca aatgaccgcc gagatcatgt
     9601 accagatggc tgcgctgctg ccgccgcact tccgcggtca ctatgccgat atgaagcagg
     9661 ccacccaggc ctatttgcgg tttggcccgc aggccgcgct tcaattctaa atttgtccat
     9721 tcattggaca ttttgtccaa aatttgtcca gaccccggtg cataataaga tta
//
LOCUS       DAOPVS010000070         9494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7258_length_9494_cov_59.791291, whole genome shotgun sequence.
ACCESSION   DAOPVS010000070 DAOPVS010000000
VERSION     DAOPVS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7258_length_9494_cov_59.791291"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="msrP"
                     /locus_tag="PKL11_06960"
     CDS             complement(<1..665)
                     /gene="msrP"
                     /locus_tag="PKL11_06960"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNZ13380.1"
                     /translation="MNKIPSSEITPENVYMSRRSFLKRAGLLAAAAAALAACKPQGAV
                     LPPADATPPSASGQTDELGDALTDYQDVTTYNNFYEFSLDKEAVARLSEGWITSPWSV
                     TVGGLVNRPKTYDVDDLRRLYDEEERIYRMRCVEGWSMVIPWLGFPLSKLLKDVEPTG
                     QARYVRFESVYDPAGLPGQRDPWFNWPYVEGLRLDEAMHDLTILSTGLYGKTLLPQNG
                     APLR"
     gene            complement(746..1324)
                     /locus_tag="PKL11_06965"
     CDS             complement(746..1324)
                     /locus_tag="PKL11_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ13381.1"
                     /translation="MTTPPLTPRQKTIELARQMVEAKPVYLDTETTGLNKTDEIVEIA
                     VVDWDGSILFETLVRPSQPIPAEAMRVHGITDEMVKPARPWPIVWQQLRPLLLGRVVA
                     IYNAEFDLRMMQQSMEQYRMPWREQFNALDVMRLYSDYRGEWDPMRRANRRFRLAQAG
                     EAFNIKLPNAHRGAADSLLTRALLHAMAGVPY"
     gene            complement(1321..2196)
                     /locus_tag="PKL11_06970"
     CDS             complement(1321..2196)
                     /locus_tag="PKL11_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13382.1"
                     /translation="MQKVWLLLIALTLMLTACNLPVGNTPAPSDADEIATRVAETLTL
                     AAGGAITPLPPVEQTPEAATATPALPPAEPTLTETPQPTLTPTPTATVNNSDPRQSLG
                     APAWQDTLDDGGAWGLDSTGYQDDYLGIKIENGSMFLTSYQALGWHSWRLNGQRPQNF
                     YLEATLRPQNCSGSDAYGLVFRAPDYDSGRGYYLRLRCNGQFVLSAWPDDGMDNLTEW
                     QTHSTFVGGANQTNRIGVLVNGSSLAVYANGTLVQTVTDSSFSDAGHIGVFISAVSTP
                     GFTVEMSEISYWNLP"
     gene            2307..3797
                     /locus_tag="PKL11_06975"
     CDS             2307..3797
                     /locus_tag="PKL11_06975"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNZ13383.1"
                     /translation="MRTHIIQLETHDDVISTCDKMGWSKSRRVLLIWPRLSGVLERKL
                     DLVILLRHARQLGMQLAIVSRAPDVQQQARALGIPLFQTASEAQRRAWRRPRRRRAFR
                     QPLSRSDLSQLRSAVSSDAPLQPDRLSRLLVFSLGVLAALALVLFFLPSARITLPLSQ
                     QSQSLDLPVFASPALTTVGLSGGIPAREVTVIVEAQQTTPSSGQSAVGLTYATGTVTF
                     TNLNGLSLTIPADTVVTTADDPAIRFATTRQVTLPAGPGQTVDAPVQALVPGASGNLA
                     SGAIAAVEGQLGLQMTVTNAAALRGGTDRLARLPSESDYADLRAALLDSLEQTALANL
                     TELLGEGQQILPGTLALSEVQYENQSPPVGLPGDQLTLTLRVEYSAWAVRYTDVEQAA
                     QLALDASLPSGFRARPNSLQWTTLTQPTLSTDGLLHWTLRAERQLQADYSEDAVIANV
                     LARSPRQAAAWVNTNISLNAPAIVALWPPRWPWMPALPFRIQVDTQ"
     gene            3794..4237
                     /gene="ruvX"
                     /locus_tag="PKL11_06980"
     CDS             3794..4237
                     /gene="ruvX"
                     /locus_tag="PKL11_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNZ13384.1"
                     /translation="MNNPETRILAVDPGEKRIGLAISDPTATIARPLTVVKHVSRLLD
                     AAEVARLALENNVVRIVIGCPLDSEGQPGPAARRSERFADALRQQTDIPILLWDESGS
                     TQTARAVRLEMGVRRRDRQGHLDDLAAVIILQSYLEARREAGEAP"
     gene            4234..5376
                     /gene="mltG"
                     /locus_tag="PKL11_06985"
     CDS             4234..5376
                     /gene="mltG"
                     /locus_tag="PKL11_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNZ13385.1"
                     /translation="MTGRSSEGCGRPVVLLLLVVLCMFVVGGLLFLERLPTAVARNFG
                     PPSPALSAPNRIWYAAQLELRRQELLLPRDPTGPAQTFEISSGESVDAIATRLESTGL
                     IWKADIFRVYMIYAGLDRSIQAGSYQLSPAQTPLEIAHAMQDATPSEVSFYLLPGWRA
                     EEVAAALPLNGLSIDPQTFIALVQNPPLSILPPGWPVSGGLEGYLYPGEYQLRRDYSA
                     EQILQTFLLRFDEQVPMDLRVEFERRGLSLAEAVTLASIVQREAVVEDERPLVASVFY
                     NRLAAGMTLGSDPTVQYALGYNLAQSTWWTNPLSIDDLQVYSPYNTYQVTGLPPGPIC
                     SPAISSLQAVAYPADSPYYYFRALCDGSGRHAFAVTYDEHLQNACP"
     gene            5391..6137
                     /locus_tag="PKL11_06990"
     CDS             5391..6137
                     /locus_tag="PKL11_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ13386.1"
                     /translation="MSTILGLDVGGSGIKGALVNIETGELVAPRHRLPTPEAAKPAAV
                     GEVLKQLTLHFQYSGPVGIGFPSVIRHGVILTAANVDSSWIGLPANEFFGKAIGMPAH
                     ALNDADAAGIAEMAFGAGRAYHTGVVLLLTVGTGIGSAVFVNGHLLPNTEFGHIQIRG
                     KDAERRASDAARQRKKLSWEQWATRFEEYLETMCALISPDIIILGGGVSRKMDKFMPH
                     IKVDVKIIPAELENDAGIVGAAMFAAHPSA"
     gene            complement(6134..7579)
                     /locus_tag="PKL11_06995"
     CDS             complement(6134..7579)
                     /locus_tag="PKL11_06995"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNZ13387.1"
                     /translation="MTPTGSLYRIERDSLGEVQVPKNALYGAQTQRAVVNFPISGLRP
                     RRAFVWSVAAIKCAAAKVNRDLGLLDEDLAEGIIAAATEVMEDLWADQFVVDPFQAGA
                     GTSHNMNANEVIANRATALLSRLTGAQVHPNDHVNMSQSTNDVIPTAIRLGCLWRLDE
                     LLGAVTALAETLQGKAQEFDDVIKSGRTHLQDAVPVRLGQEFGAYARAVARDAERIAR
                     AAEGLRRIGIGGTATGTGLNAHPEYHARMVGTLSAMTGIKLYESDNLFESMQSMADMA
                     DFSAALRTLAITLTRIANDFRLLSSGPNTGLDEIRLPAMQPGSSIMPGKVNPAMAEML
                     NMTMFQVMGCDATISLAAQAGQLELNVMMPVIAHNLFEMMHLTIQAVRAFTEKCVAGA
                     TANREKASRWLQNNPILITALNPIIGYVAGAALVKEAQERGIPIRDLALEKAEHGALH
                     HRETQQPVSPEEIRAIFEDLRQFTEGGIAGS"
     gene            complement(7672..8082)
                     /locus_tag="PKL11_07000"
     CDS             complement(7672..8082)
                     /locus_tag="PKL11_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ13388.1"
                     /translation="MPHVITSLCLRDGGCATVCPVECIVPGKPVDKWPLYYIDADTCI
                     DCGACVSECPWGAIFPVEELPAPYVAKGGEYLSEPVGTAGYETVYDGKNHDGDAVHLT
                     ATRILAAGEKVDLTGFADVNAAFFKEGPGYSAKD"
     gene            complement(8120..8731)
                     /locus_tag="PKL11_07005"
     CDS             complement(8120..8731)
                     /locus_tag="PKL11_07005"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ13389.1"
                     /translation="MPYELPALPYALDALEPWIDARTVEIHYGKHHQTYLSNTNKALE
                     AHPELFAFSIEDLLRDLNRVPEAIRTAVKNNGGGVYNHNIYWQGLGPNCGGEPVGKLA
                     EAIQATFGDFTTFKAEFEKAGLTRFGSGYAWLSRRADGSLLIHSTANQDCPLSEGLYP
                     ILTVDVWEHAYYLKYQNRRAEYLSIWWNLVNWKQADERFTHGK"
     gene            8998..>9494
                     /locus_tag="PKL11_07010"
     CDS             8998..>9494
                     /locus_tag="PKL11_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNZ13390.1"
                     /translation="MRFAQTEASSIVIAAYRLGLAALILAVMVLPRQWAAIRALPRRT
                     LALAALAGLFLAFHFASWITSLEHTTIASSVVLVTTAPLWVAMLSGLVLHERIAPAVW
                     LGLGVALLGGVIVGVSGECTLTSGQIACPPLSYFASGKNTLGNLLALFGAWMSGAYLM
                     VGRKVR"
BASE COUNT         1759 a         3058 c         2569 g         2108 t
ORIGIN      
        1 cgcagtggcg cgccgttttg tggcagcagc gttttgccat acagcccggt ggataagatc
       61 gtcaggtcgt gcatcgcctc atccagccgc agcccctcca catacggcca gttgaaccac
      121 ggatcacgct gccccggcaa acccgccggg tcgtacacgc tttcaaagcg cacatagcgg
      181 gcctgtccgg tgggctccac atccttcagc agcttgctca gcggaaaacc cagccacgga
      241 atgaccatgg accagccttc aacacaccgc atccggtaga ttcgttcttc ctcgtcatac
      301 agccgccgca gatcatccac atcatacgtc ttgggccggt taaccagccc tccaaccgtg
      361 acgctccagg gcgaagtgat ccagccttca gataaccggg ccactgcctc tttatccagg
      421 ctgaactcat aaaaattatt gtaagttgtc acatcctgat agtcggtcaa cgcatcgccc
      481 agctcgtccg tctggccgct ggcgctgggc ggtgtcgcat ctgccggggg caaaacagct
      541 ccctggggtt tgcaggctgc cagtgccgct gcggctgcag ccagcaggcc agcgcgcttc
      601 aaaaaactgc gccgagacat atagacgttt tcgggcgtta tctcagaaga agggatcttg
      661 ttcattttta cctctaataa cttaccaata agttgcaggc ccttattctg gctgcagtgc
      721 tgccgtgatg ttagaaccgg gtcttctaat aaggcacgcc cgccatggca tgcagcaaag
      781 cacgggtcaa cagcgagtct gccgcacccc ggtgggcatt gggcagcttg atgttaaacg
      841 cctcgcctgc ctgcgccagc cggaaacgcc ggttggcgcg gcgcattggg tcccattcac
      901 cgcggtaatc ggaatacaac cgcatcacat ccagcgcgtt gaactgctca cgccacggca
      961 tgcggtattg ctccatcgat tgctgcatca tccgcaggtc aaactctgcg ttatagatcg
     1021 ccaccacgcg ccccagcagc agggggcgca gttgctgcca cacaatcggc cacgggcgcg
     1081 ccggcttcac catctcatcg gtgatgccgt gaacacgcat cgcctcagcc gggatgggtt
     1141 ggcttggccg caccaaagtt tcaaacagga tgctgccatc ccagtccaca accgcaattt
     1201 ccacaatttc atccgtcttg ttcaggccgg tggtttcagt atccaggtaa accggtttgg
     1261 cttccaccat ctggcgggcc agttcaattg ttttttgacg tggggtgaga gggggagttg
     1321 tcatggtaga ttccagtagc tgatttcgct catttcaacg gtaaatccgg gtgtgctgac
     1381 tgcagaaata aacacgccaa tatgcccggc atcgctgaag ctgctgtccg tcacggtttg
     1441 caccagcgta ccgttggcat acacagccag cgaactgcca ttcaccagca caccaatccg
     1501 gttggtctgg ttggcgccgc ccacaaacgt gctgtgggtc tgccattcgg tcagattatc
     1561 catgccatca tccggccagg cgctcaaaac aaactgcccg ttgcagcgca gccgcaggta
     1621 atatccccgt ccgctgtcat aatcgggggc tcgaaacacc agcccatagg cgtcagatcc
     1681 gctgcagttt tgtgggcgca gcgttgcttc caggtagaaa ttctgcgggc gctgcccgtt
     1741 caaccgccaa ctgtgccagc caagggcctg ataactggtc agaaacatac tgccattttc
     1801 aatcttgatg cccaggtaat cgtcctggta gccggtgctg tccaatcccc aggcgccgcc
     1861 atcgtccagt gtgtcttgcc acgccggcgc ccccagtgac tgtcgcggat cgctgttgtt
     1921 cacggtagcg gttggtgttg gcgtcagcgt tggctgcggc gtttctgtca gggtcggttc
     1981 tgccgggggc agggcggggg tggcggttgc cgcttccggg gtctgctcca caggcggcag
     2041 cggtgtgatg gcgccccccg ccgccagcgt cagggtttca gccacccggg ttgctatctc
     2101 atcagcatca gagggtgccg gtgtattccc caccggcagg ttacaggctg tcagcatcaa
     2161 tgtaagagca atcaatagca gccagacttt ttgcatggtc aaattccttt ctcaacaatc
     2221 ctggtctgat tatagccgtt aagcagttac aaaaaaagtc ccagtccaac cttcaaccgg
     2281 cattggtgtt gtataatggg gaacgaatgc gcacacatat cattcagctt gaaacccacg
     2341 atgatgtcat ctccacctgc gataaaatgg gctggagcaa atcccgccgc gtcctgctca
     2401 tctggccgcg cctgtctggc gtgctggagc gcaagctcga cctggtgatt ttgctgcggc
     2461 atgcccgcca gttgggcatg cagttagcca ttgtcagccg cgctcccgat gttcagcagc
     2521 aggcgcgggc gctgggcatc ccgcttttcc agaccgccag cgaagcccag cggcgggcct
     2581 ggcgccgccc gcgccgccgc cgggcatttc gccagccatt atcgcgttca gacctgagcc
     2641 agctccgctc tgcagtgtca tccgatgccc ccctgcagcc tgaccgcctc tcccggctgc
     2701 tggtgtttag cctgggtgtg ctggcggcgt tggcgctggt gctctttttc ctgccatctg
     2761 cgcgcattac cctgccgctc tcccagcagt cgcaatcgct cgatctgccg gtttttgcca
     2821 gccccgcgct gaccaccgtc ggcctcagcg gcggcatccc cgcccgtgag gtcacggtca
     2881 ttgttgaagc ccagcagacc actcccagtt cagggcagtc ggcggttggc ctcacctatg
     2941 ccacaggcac agtcaccttc accaatctca acggcctgtc tctgaccatc cccgccgata
     3001 ccgtggtcac cactgccgat gatcccgcca ttcggtttgc caccacccgc caggtcacgc
     3061 tgccagccgg ccccggtcag acggtggatg ccccggtcca ggcgctggtg ccgggggcct
     3121 cgggcaatct ggcttcgggc gccatcgccg ccgtggaagg gcagttgggc ctgcaaatga
     3181 ctgtgaccaa tgccgctgca ctgcgcggtg gaacagaccg cctggcgcgt ctgccttctg
     3241 aatctgatta tgctgacctc cgcgcggccc tgttggattc tcttgaacaa accgccctgg
     3301 caaatctgac cgagctgctg ggcgaaggcc agcagatttt acctggcaca ctggccctgt
     3361 ctgaagtgca gtatgaaaac cagtctcccc ctgtaggcct acctggcgac cagttgaccc
     3421 tgaccttgcg agtggaatac agcgcctggg ctgtgcgcta taccgatgtg gagcaggccg
     3481 cccaattggc gttggacgcc agtctgccgt caggttttcg cgcccgccct aattcgctcc
     3541 agtggaccac cctgacccag cccaccctgt ccaccgatgg cctgctgcac tggacgctgc
     3601 gcgctgaacg tcagctccag gccgattatt ctgaagatgc tgtcattgcc aatgtcctcg
     3661 cccgctcacc ccgtcaggct gccgcgtggg tcaataccaa catctcactg aatgccccgg
     3721 ccatcgtggc gctttggcct ccgcgctggc cctggatgcc cgccctgcct ttccgcattc
     3781 aggtggatac acaatgaaca accccgaaac tcggattttg gccgttgatc ccggtgaaaa
     3841 gcggattggc ctggccatca gcgaccccac cgccaccatt gcccgtcctc tgaccgtagt
     3901 caaacacgtc tcccgcctgt tggatgcggc cgaggtggcg cgactggccc tggaaaacaa
     3961 cgttgtgcgc atagtcattg gttgcccgct ggattccgaa ggtcagcccg gcccggcagc
     4021 ccgccgttca gagcgttttg ccgacgctct tcgccagcag accgatatcc ccatcctgct
     4081 ctgggacgag agcggcagca cccagaccgc ccgcgccgtt cggttggaaa tgggggtgcg
     4141 ccggcgtgac cgtcagggcc atctggacga ccttgccgcc gtgattatcc tgcaatccta
     4201 ccttgaagca cgccgcgaag ccggagaggc cccatgaccg ggcgcagttc ggaagggtgt
     4261 ggccgtccag tagtgctgtt attgctcgtg gtgctctgca tgttcgttgt cggcggtctg
     4321 cttttcctgg agcgcctgcc caccgccgtt gcccgaaact ttggcccccc cagtccagct
     4381 ttgagcgccc ccaaccgtat ctggtatgca gcccagcttg agcttcgccg ccaggaatta
     4441 ctcttgccgc gcgatcccac cgggcctgcc cagacttttg aaatcagctc tggcgaatcg
     4501 gttgacgcca tcgccacccg tctggaatca accggcctga tctggaaagc cgacatcttc
     4561 cgggtctata tgatttatgc cggtctggat cgcagcattc aggctggcag ttaccagctt
     4621 tctccggcac aaacaccgct tgaaattgcc catgccatgc aagatgccac cccgtccgaa
     4681 gtgagctttt atctgctgcc cggctggcgc gcagaagagg ttgccgccgc cttgccgctc
     4741 aacggcctat ccattgaccc tcaaaccttt attgccctgg ttcagaaccc gccgctctcc
     4801 attttgcccc cgggctggcc cgtctccggt ggtctggagg gctacctcta tccgggtgaa
     4861 tatcagcttc gccgggatta ttccgctgag cagattttgc agaccttttt gctgcgcttt
     4921 gatgaacagg tccccatgga tttgcgcgta gaatttgaac gccgcggtct ttcgcttgcc
     4981 gaggcggtca cactggcctc cattgttcag cgtgaggccg ttgttgaaga tgaacgtccg
     5041 ctggtcgcct cggtttttta taaccgtctg gctgctggca tgactctcgg cagtgatccc
     5101 accgttcaat atgcgctggg ctacaattta gcccaatcca cctggtggac caatccactc
     5161 agcattgatg acctgcaggt ttattcgccc tacaatactt atcaggtcac tggcctgccg
     5221 cctgggccca tttgcagccc ggcaatttcc agccttcaag cggttgctta tccagccgat
     5281 tcgccgtatt attattttcg agccctgtgc gatggctcag ggcggcatgc ctttgccgta
     5341 acctatgacg aacatttgca gaatgcgtgt ccataactgg aggtgaagca atgtccacga
     5401 ttttaggcct ggatgtcggc ggctcaggca tcaagggtgc cctggtgaat attgagaccg
     5461 gcgagctggt tgccccgcgt catcgtttgc ccactcccga ggccgccaaa ccggcagccg
     5521 tgggagaagt cctcaagcaa ttgaccctcc attttcaata ttccggaccg gttggcattg
     5581 gtttcccctc cgtcatccgg catggcgtca tcctcaccgc cgccaacgtt gattcaagct
     5641 ggattggcct gcccgccaat gaattttttg gcaaggccat tggcatgccg gctcatgcac
     5701 tcaatgatgc tgacgccgcc gggattgccg agatggcttt cggcgccgga cgcgcttatc
     5761 acaccggcgt tgtgctttta ctgacggttg gaacggggat tggctcggcc gtttttgtca
     5821 acgggcatct cctgcccaat acagagtttg gtcacattca aatccgcggc aaagatgctg
     5881 agcgccgcgc ctcagatgca gcccgccagc gcaaaaagct ctcctgggag cagtgggcca
     5941 cgcgttttga agaatacctg gaaacgatgt gcgccctgat ctctcccgac atcatcattc
     6001 tgggcggggg cgtcagccga aaaatggaca aattcatgcc gcacatcaag gtggatgtca
     6061 aaatcatccc cgccgaactt gaaaacgatg cgggcattgt tggggcagcc atgtttgccg
     6121 cccatccgtc cgcttaactc ccggcaatcc cgccctcggt gaactgccgc aggtcttcaa
     6181 atatcgcccg gatctcttcc gggctcacag gttgctgcgt ctcacggtga tgtaacgccc
     6241 cgtgttcggc tttctccagc gccaggtctc gaattggaat cccgcgctcc tgcgcctctt
     6301 tgaccagcgc cgccccggcc acatatccaa taatcggatt caatgccgta atcaagatgg
     6361 ggttgttttg cagccagcgg ctggctttct cacggttggc tgttgcgcct gccacgcact
     6421 tctcagtgaa cgcgcgcacc gcctggatgg tgagatgcat catctcaaat aaattgtgcg
     6481 caatcaccgg catcatcaca ttcaattcca gttgccctgc ctgcgccgcc aggctgatgg
     6541 tcgcatcgca gcccatcacc tggaacatgg tcatattcaa catttcagcc atcgccgggt
     6601 tgaccttccc cggcatgatg cttgagcccg gctgcattgc cggcagccga atttcatcca
     6661 gccccgtgtt gggcccggag gataacagcc gaaaatcgtt ggcaatgcgc gtcagcgtga
     6721 tcgccagtgt gcgcagcgcc gccgaaaagt ccgccatatc cgccatggac tgcatgctct
     6781 caaacaggtt gtcggattca tacagcttga tgcccgtcat tgcgctcagg gttccaacca
     6841 ttcgggcgtg atattccggg tgagcgttca accctgtgcc cgtggcggtt cccccaatcc
     6901 cgatccgcct caggccttcg gccgcccggg caatccgctc ggcatcgcgc gccaccgccc
     6961 gggcataagc gccaaactcc tgccccagcc gcacgggcac cgcatcttgc aggtgagtgc
     7021 gccccgattt gatcacatcg tcaaattcct gcgctttgcc ttgcagcgtc tctgccagcg
     7081 cggtcacggc cccgagcagt tcatccagcc gccacaggca ccccagccgg atggcggttg
     7141 ggatcacgtc attggtcgat tgtgacatgt tcacatgatc gttggggtga acctgtgccc
     7201 cggtcaaccg gctgagcagg gcagtggccc ggttggctat cacctcgttg gcattcatgt
     7261 tatggctggt gcccgctccc gcctggaagg gatcgaccac gaattgatcc gcccacaggt
     7321 cttccatcac ttcggtcgca gccgcaataa tcccctctgc caggtcttca tccaacagcc
     7381 ccaggtctcg attcaccttg gccgccgcgc atttaattgc cgccacagac cagacaaacg
     7441 cccgtcgggg tcttaaaccc gagatgggaa agttaaccac tgcccgctgg gtttgcgccc
     7501 catacagcgc atttttgggc acctgcacct cacccagtga gtcgcgttca atccgataga
     7561 gcgaacctgt tggagtcatg ggtcacctcg ccttaacagg cagggcggac acaaatgtcc
     7621 gccccgccat ttctaacctt taaactttac ggcagacaga tcacatactg cctaatcttt
     7681 ggcgctgtag ccagggcctt ccttaaagaa tgccgcatta acatctgcaa aaccggtcag
     7741 gtccactttt tcaccggcag ccagaatccg ggtggcggtc agatgcaccg cgtcaccgtc
     7801 atgattcttg ccatcataga ccgtctcata cccggctgtg cccaccggct cagacaggta
     7861 ctcgccgcct ttggcaacgt agggcgccgg cagttcttcc accgggaaga tcgcccccca
     7921 cgggcattcc gagacgcagg ccccgcagtc aatgcaggta tcggcatcaa tataatagag
     7981 cggccacttg tccaccggct tgccgggaac aatgcactcc acagggcaga cggtggcgca
     8041 gccaccatcc cgcagacaca ggctggtaat tacatgcggc atttttacac tccttaaaat
     8101 ggttagagat cagggaaact tatttcccgt gcgtgaaccg ctcgtcagct tgtttccaat
     8161 tgacgagatt ccaccagatg gaaagatact ctgcccggcg gttttgatac ttcaggtaat
     8221 aggcatgttc ccacacatcc acagtcagga tggggtacag cccttccgaa agcgggcagt
     8281 cctggttggc tgtgctgtga atcagcagcg agccatcggc gcggcggctg agccaggcgt
     8341 aaccgctgcc aaagcgcgtc agcccggctt tctcaaactc ggccttaaag gtggtaaaat
     8401 cgccgaatgt ggcctgaatg gcctcagcca gtttgcccac cggttcgccg ccacagttcg
     8461 gccccagccc ttgccagtag atattgtgat tatagacccc gccgccgttg ttcttcaccg
     8521 ccgtgcggat cgcctcgggc acccggttca ggtcccgcag caaatcctca atcgagaagg
     8581 cgaacagttc cgggtgtgct tccagagcct tgttggtatt gctcaggtaa gtttgatgat
     8641 gcttgccgta atggatttca accgtccggg cgtcaatcca gggttccagc gcatccaggg
     8701 catagggtag tgcaggcagt tcgtaaggca taggtcaccc caaaaaatga attttgaaca
     8761 gtaggttttg gaaatttcaa acgacatacc gattgtagcc agcgcatgaa tgtttgtcaa
     8821 gcattcctga tgatttttac cccctttggc attgccggat tttgcaggcc caaaatgccc
     8881 ccaatcattg ccttgcttct ttagaaggag tcccccatcg ccccgtctgc ccccgccaaa
     8941 acccccttcc tggccctcat cctgggcatc ctggctgttt ccacggcctc tgtcctgatc
     9001 cgctttgcac agaccgaggc gtcttctatt gtcattgccg cctatcgcct gggcctggct
     9061 gccctgatcc tggccgtcat ggttctcccg cgccagtggg ccgccatccg cgccctgccc
     9121 cggcgcacgc tggccctggc tgccctggct ggtctcttcc tcgcttttca cttcgcttca
     9181 tggattacat ctctcgaaca taccaccatc gccagttcgg tggtgctggt caccaccgcg
     9241 ccgctctggg tcgccatgct ctccgggctg gttctgcatg agcgcattgc cccggcggtc
     9301 tggctgggcc tgggggtagc cctgctgggt ggtgtgattg tcggcgtcag cggagaatgt
     9361 accctcacat ccggtcaaat tgcctgcccg cctctgtcat atttcgccag cggcaaaaac
     9421 actctcggca atcttctggc tctcttcggc gcctggatgt ccggcgccta cctgatggtc
     9481 ggacgcaaag ttcg
//
LOCUS       DAOPVS010000071         9363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7384_length_9363_cov_56.424688, whole genome shotgun sequence.
ACCESSION   DAOPVS010000071 DAOPVS010000000
VERSION     DAOPVS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_9363_cov_56.424688"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1480
                     /locus_tag="PKL11_07015"
     CDS             128..1480
                     /locus_tag="PKL11_07015"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNZ13391.1"
                     /translation="MSQTRTACPRCRTPLLAEVEQVVDVAVDPQAKQRLLSGSLNVIE
                     CQNCGYSGMLATPLVYHDPEKELLLTYFPPELGLPINEQERVMGPLISQVVNRLPAEK
                     RKAYLLRPQLMLTFETLLNRVLEADGITREMIEDQQKRLNLLRRLLSTTQSETRVEII
                     KQEEALIDENFFALLGQIGESAVHNNEQQMAQALMMLQQELLENTELGRKLKERAEET
                     QAAIQSLQAASQAGLTREKLLDLIVEAPTETRRAALVGMARSGLDYQFFQILSDRIEA
                     AAGPQREQLTALRDQLLEMTKAIDAEVQTQVQAAEDLLNSLVTAENIEAATEEHLPEF
                     NDFFINVLKAELQAAQQKGDQARLEKLQRVVMVLQHASARPEMELVEKLVSAGTDEAR
                     RTVLQANAEMVTPEFLEILNRLAVQSEAEGQQPEIVAALQAAYRSAMRFSMEQKFKQN
                     "
     gene            complement(1610..2281)
                     /locus_tag="PKL11_07020"
     CDS             complement(1610..2281)
                     /locus_tag="PKL11_07020"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNZ13392.1"
                     /translation="MREIPPEVPILIAQTGPLNGQRWPLTQTAVIGRDPDCDIVIADR
                     QISRFHARLTPDADGVILEDLGSKNGTYCNGSVLTESVRLQDGDQVQIALAQHFVYLS
                     SDATLPLDGTLPAMAAVQSSPLTLDAPSRRVWVGGVEIVPPLSAPQFRLLQILYTHAG
                     QVVSRADLVETVWTEAEAEGVSEQALDALIRRLRDRLADADPQHNYLTTVRGHGVRLD
                     LGSSE"
     gene            2411..3397
                     /locus_tag="PKL11_07025"
     CDS             2411..3397
                     /locus_tag="PKL11_07025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ13393.1"
                     /translation="MPLPLKAGEVLRSRYRIRRIIGQGGMGSIYLADDQRLEGRQCAL
                     KEVEHDRTLPTELVREAREQFLREATVLARLDHPNLPKVSDFFSIGSRDYLVMDYVPG
                     KDLRALMLEARQDNRFLPEADVLNWAAQLCDALTYLHSQEPPILHRDIKPSNLKVTPS
                     GLLKLVDFGLVKLLAPGEMTITILQGQGTALYTPLEQYGGDSGHTDVRSDIYAFGATL
                     YHLLTNTQPADARERFLSPESLTPPRKINPDISSRSERAILWAMGVHPDERPETVERF
                     RQTLLGDQPTLPRPTINRTVQLKDLLEQPTESGLMWALALLTLVTFVLTVSR"
     gene            complement(3411..6155)
                     /locus_tag="PKL11_07030"
     CDS             complement(3411..6155)
                     /locus_tag="PKL11_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ13394.1"
                     /translation="MTPRRSLLLLTLIALLAAALFPSTRALAQTDDPRARAQALLAQM
                     TPQEKVGQLFLVTFEGLNVDTDSQIYDLIVNQHVGGVVLSADNDNFTGPEGTAVAAQN
                     LITALQTSAAETNGVYVPLFVGVSQEGDLAPYDSIFNDLTPLPNQMAIGATWKPALAQ
                     QVGTVMGRELEALGFNLYLGPSLDVLDVLRSETSDDLGTRTFGGDPYWVGAMGSAYIN
                     GLHTGSDYRLAVIAKHFPGRGGSDRPPEEEVATVRKSLESLKQIELAPFFAVTGNAPD
                     AASTTDGLLVSHIRYQGFQGNIRATTRPVSLDAAALEQILTLPELTPWRDNMGIIVSD
                     DLGSQAVRRFYDPTGRTFDARAVARSAFLAGNDLLYVNEFTATGDPDTYTTLLSTLES
                     FTQKYREDAAFAERVDASVERILTLKYRLYGEFALENILPTADSLTLVGQAQTVSFEV
                     ARQGVTLLSPTAAQLSLDLPRPPTANDRILFLTDTYNARACTNCPEQTLLGVSDLQLA
                     VQRLYGPQGGGQISASRLYAYSFRDLKALLDDPLAQPTLQTDLQSANWVVVLMLGEDA
                     DRPASTAFRQMLSQRPDLLAGKHVIVFALNAPYYLDATDISVLTAYYGLYSKSPDFVN
                     VAARILFQEISPVGALPVTVPGVGYDLIDATSPDPTQVIPLYLDLPAATATPNAAGTL
                     EPTPAPSYLIGDTIPLRTGVILDRNRHTVPDGTVVRFVFELGGETTTTQQIETVTMQG
                     VAHASYRIQSAGALQIRVISDPAYSSDIIQLNVAENQGAVPTVLVATPEPTITPTPEP
                     TATPSPTPQAPPLTTPPPTLTGLHWLLAAALAWGGAAAIYGLGRKRATLRWGLRWALL
                     AGLGGLLAYFLLLILSRTFPTWIESARLGATLGFTILGVLLGWLAGWIWQQAMLRRRR
                     "
     gene            complement(6152..7108)
                     /locus_tag="PKL11_07035"
     CDS             complement(6152..7108)
                     /locus_tag="PKL11_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ13395.1"
                     /translation="MIPPEWLDAAAARLAGRVRITPVTYDAELDLYLKWENRQTTGSF
                     KLRGAANKVLSLQPWERARGLVCASAGNHGQGVALAAQEVAAPVIVFASDHATPLKLA
                     AMRALGAEVRTVPGGYELAERTAHAFAVEQGYAWVSPYNDGQVIAGQATLGLELLEQL
                     SPFPAQVCLVPVGGGGLISGIGLALRRLQHPPRLIGVQSQASAFFHGLFHRGTQADVI
                     ETDSLADGLSGAVEADALTIPLVRSLVDDLVLVSEEQIGQAVAFAWQRYGEVIEGSAA
                     VALAAVLCGQVMARPALVVLTGGNIQPETHLALLQRYGAAAS"
     gene            complement(7105..7863)
                     /locus_tag="PKL11_07040"
     CDS             complement(7105..7863)
                     /locus_tag="PKL11_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ13396.1"
                     /translation="MNLLTDLPRPVFFAHRGACAHAPENTLASFRLAVEHGAPAVELD
                     AKLSADGQVVVIHDPTINRTTDGKGFVNHLPLAALRDLDAGSFFAPRFRGERIPTLEE
                     VFETVGRNILVNVELTNYTSPLDALTIKVAELIKRLNNADQVIFSSFQPLNLVRIRQM
                     LPQVPVAILFSQGAARRKTTAWLGRRVAPEIVHPYLTDATADFIQHQHTLKRRVHVWT
                     VNDPQEMRRLFAADVDGIFTDDPLLARQTLENMA"
     gene            complement(7860..9215)
                     /locus_tag="PKL11_07045"
     CDS             complement(7860..9215)
                     /locus_tag="PKL11_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ13397.1"
                     /translation="MTTMPPRPAQPPAGNAPVGTGAMSFVPPPISRMEDTGLSPLWLQ
                     DLVLKIMYFQGYMTGFRIAEDIALPFTGIVDQLLEALKREKLLEVRTSQMGLGEGAYV
                     YAITGAGIARAREALDRSQYAGPAPVPIAVYNESIRRQSRGRFQITNRLMRQVLAHLV
                     LSEKAYSRLGPAVNSGTSIFMYGPPGNGKTSVARAVGNLIQSQLMYIPYAISVDGQVI
                     KLYDSVNHQVAPEEDATPMGTGSLRTGARRDPRWMRIRRPFIITGGELTLAGLDLVFD
                     DTLKFYEAPFQMKANGGILLIDDFGRQQVRPRDLLNRWIVPLENRIDYLALHTGRKIE
                     VPFDVLVIFSTNLPPRELVDEAFLRRLRHKVEVGDPTYEEYREIFRRVSGQKGVQYSD
                     QGLAYLLQEHYIKRNRKLRASHPRDLCDQILDIARYLNLEPAMSRELLDRAADSYFVE
                     L"
BASE COUNT         1933 a         2596 c         3163 g         1671 t
ORIGIN      
        1 gcagggtcag gcggggtgtt tcaaggggga gagaaaaagc gggcatggat taattgtacc
       61 ctcaaaatgt gccagggtga aatctggtat aatcccctca cattctgttg gagtgagtta
      121 aaaaactatg tctcaaaccc gtactgcctg cccgcgctgc cgtacacccc tgcttgccga
      181 ggtggaacaa gtggtggacg tggctgttga cccacaggcc aagcagcgcc tgctgagcgg
      241 cagccttaac gtgattgaat gtcaaaactg cggttattcc gggatgctgg cgaccccact
      301 ggtctatcat gaccccgaaa aagaattact gctgacctac ttcccgcccg aactggggct
      361 gcccatcaat gaacaggaac gcgtgatggg accgttgatc tcacaggtag tcaaccggct
      421 gccggcagag aagcgcaaag cctacctgct gcgcccgcag ttgatgctga ccttcgaaac
      481 cctgctcaac cgtgtgctgg aagctgacgg catcacccgc gagatgattg aagaccagca
      541 gaagcggctc aacctgctgc ggcggctgct ttctacaacg cagtctgaaa cccgggttga
      601 gattatcaag caggaagaag ccctgattga cgaaaacttc tttgcgctgc tggggcagat
      661 tggcgagtcg gcggtgcata acaacgaaca gcagatggct caggccttga tgatgctgca
      721 gcaggagctg ttggagaata ctgaactggg ccggaagctg aaagaacgcg ccgaggaaac
      781 ccaggcagcc atccagtctt tacaggcggc cagccaggcg ggattgacgc gcgaaaagct
      841 gctggacctg attgtggaag cccccaccga aacccggcgg gctgcgctgg tgggcatggc
      901 ccgcagcggg ctggactacc agttcttcca gattttgagc gaccggattg aagccgcggc
      961 aggcccgcag cgcgagcagt tgaccgccct gcgcgatcag ctcctggaaa tgaccaaggc
     1021 gattgacgcc gaggtgcaga cccaggttca ggccgccgaa gacctgctga acagcctggt
     1081 gacggcggag aatattgaag cggccaccga agaacacttg ccagagttca acgatttctt
     1141 catcaatgta cttaaggctg aactgcaagc tgcccagcaa aaaggcgatc aggcccggct
     1201 ggaaaagctg cagcgggtgg tgatggtgct gcaacatgcc agcgcccgcc ccgagatgga
     1261 gctggtggaa aagctggtca gcgctggaac cgacgaggcc cggcgcacgg tgctgcaggc
     1321 caatgctgag atggtgacac cggaattttt ggagatcttg aaccggctgg ccgtgcagag
     1381 cgaggccgaa ggccaacagc ccgagattgt ggccgcgctg caagctgcct accgctcggc
     1441 gatgcgcttc tcgatggagc agaagtttaa gcagaactag ggctgacgat tttagcgtgt
     1501 gatgtgcaac cgcacgggct tttacccgtg cggttgtttg ttggatgggg gatggggatg
     1561 ggttttctcg cccggcgtgg aggctgaagc cggcacgcct cagagcgatt tattcgctgc
     1621 tgcccaggtc aaggcggacg ccgtgaccgc ggacggtggt gaggtaattg tgctgcgggt
     1681 cagcatcagc caggcggtca cgcaggcggc ggatgagggc atccaaagcc tgctcagaaa
     1741 cgccttcggc ttcggcctcc gtccagactg tttccaccag atcagcccgg ctgacaacct
     1801 gcccggcatg ggtgtagagg atttgcagca ggcggaactg cggcgccgac agcgggggga
     1861 caatttcaac cccgccaacc cagacccgtc gtgagggggc atccagagtc agggggctgg
     1921 attggactgc cgccattgct ggcagcgtgc catcgagcgg cagggtggca tctgaactga
     1981 gatagacaaa gtgctgcgcc agggcgattt gcacctggtc gccatcctgc aggcggactg
     2041 actccgtgag caccgagccg ttgcagtagg tgccattctt gctgcccagg tcttccagga
     2101 tcaccccatc ggcatccggc gtcaggcggg catggaagcg cgaaatctgc cggtccgcga
     2161 tgacgatatc gcaatcggga tcacgcccaa tgactgcggt ttgggtcagg ggccagcgct
     2221 gaccgttgag cgggccagtc tgggcaatga ggatgggaac ttcgggggga atttcgcgca
     2281 tagagataat cgcttctttt ctagtataat agtaattgag taattcgaca actgacagag
     2341 cgccgatcca gacaacgccg aattgacccg gattggagcg gctgtcgcct ttttctcagg
     2401 actgcgcccc atgcccctgc cgctaaaagc cggagaggtg ctgcgcagcc gttaccggat
     2461 tcgccggatt attggccagg gcgggatggg cagcatctac ctggcggatg atcagcggct
     2521 ggaaggacgg cagtgcgccc tcaaagaagt ggaacacgac cgcaccctgc ccaccgaact
     2581 ggtgcgggag gctcgcgagc agtttttgcg cgaggccacg gtgctggcgc ggctggatca
     2641 tcccaatttg cccaaggttt cagacttttt ctccattggc agccgggatt acctggtgat
     2701 ggactatgtg ccgggcaaag atttgcgggc gttgatgctg gaggcgcggc aggataaccg
     2761 gtttttgcca gaggccgatg tgctgaactg ggcggcgcag ctttgcgatg ctctgactta
     2821 cctgcacagt caggagccgc caatcctgca ccgtgatatc aaaccgagca atttgaaagt
     2881 gacgcccagc ggactgttaa agctggtgga ttttggactg gtgaagctgc tggcgccggg
     2941 ggaaatgacc atcaccatct tacaggggca gggcacggcg ctgtacacac cgctggaaca
     3001 gtacggcggc gacagcgggc acaccgatgt gcgcagcgat atttacgcct ttggtgccac
     3061 gctgtaccac ctgctgacca atacacagcc agccgatgcg cgcgagcggt ttctctcacc
     3121 ggagagcctg accccaccgc gcaaaatcaa cccggacatc agctcgcgca gcgagcgggc
     3181 cattctgtgg gcaatggggg tgcaccccga cgagcgccca gagaccgtgg agcggttccg
     3241 gcagacgctg ctgggcgacc aacccacact gccgcgcccg accatcaacc ggacggtgca
     3301 attgaaagat ttgctggagc agcccaccga gagcggctta atgtgggcac tggcgctgct
     3361 gacgctggtc acatttgtgc tgacggtttc gcgataaggg ggcgatccgt ttaacgccgg
     3421 cggcgcagca tggcctgctg ccagatccag ccagccagcc agcccagcaa gacccccagg
     3481 atggtgaagc ccaatgtggc acccagccgg gcagattcga tccaggtggg gaaggtgcgc
     3541 gagaggatga gcagcaagaa gtaagccagc aaaccaccca ggccagccaa cagcgcccag
     3601 cgcagacccc agcgcagggt tgcacgctta cgccccagcc cgtagatggc ggctgcgccg
     3661 ccccaggcca gggctgccgc cagcagccag tgcaaaccgg tcagcgtagg cggcggggtg
     3721 gtcaatgggg gcgcctgggg ggtaggtgaa ggggtggcag ttggttcggg ggtgggggta
     3781 atggttggct caggggtggc gaccagcaca gtgggcaccg cgccctgatt ttcagccaca
     3841 ttcaattgaa tgatatcgga agagtaagcc gggtcactga tgacgcgaat ttgcagcgcc
     3901 cccgcagact ggatgcggta gctggcatgg gccacaccct gcatggtcac ggtttcaatc
     3961 tgctgggtgg tggtggtttc gccgcccagc tcaaaaacga accgcaccac ggtgccatct
     4021 ggcacggtgt ggcggttgcg atccagaatc acaccggtgc gcagggggat ggtatcgccg
     4081 atcaggtagc tgggggcggg cgtgggctcc agggtgccag cggcattggg ggtggcggtg
     4141 gctgcgggca gatccagata aagcgggatg acctgtgtcg ggtcgggtga ggtggcatca
     4201 atcaaatcgt agcccacgcc ggggacggtg acgggcaaag cgccaaccgg tgaaatttct
     4261 tgaaaaagga tgcgcgcggc cacattcaca aagtctggcg acttgctgta aaggccgtag
     4321 taggcggtca ggacggaaat atcggtggca tccaaatagt agggggcatt gagggcaaag
     4381 acgatgacat gcttgccggc gagcaggtca gggcgttgtg agagcatctg ccggaaggct
     4441 gttgaagccg gacggtcggc atcttcgccg agcatcaaga ccaccaccca gttggcgctc
     4501 tgaaggtcag tttgcagagt gggctgcgcc agcgggtcat cgagcagggc cttgaggtct
     4561 ctgaaggaat aggcatagag acgagaagcg gaaatctgcc cgccgccttg agggccatac
     4621 agccgctgaa ccgcgagctg caggtcgctg acgccgagca gggtttgctc cgggcagttg
     4681 gtgcaggcgc gggcgttgta agtgtcggtc aagaagagaa tgcggtcgtt ggcggtgggt
     4741 gggcggggca ggtccaggct caactgggca gcggtggggc tgagcagggt gacaccctgg
     4801 cgggcgactt caaaagagac ggtttgagcc tgcccaacca gcgtcagaga atcggcggtg
     4861 gggaggatgt tttcaagggc aaattcgccg tagagccggt atttgagggt caggatgcgt
     4921 tcaaccgagg catccacgcg ctcggcaaag gcggcgtctt cacgatactt ctgggtgaag
     4981 gattccagcg tagagagcag cgtggtatag gtatcggggt cgccggtggc ggtaaactca
     5041 ttcacataga gcaggtcgtt gcccgccagg aaggcgctgc gtgccacagc gcgggcatca
     5101 aacgtgcgcc cggtggggtc ataaaagcgg cggactgcct gactgcccag gtcgtcgctg
     5161 acgataatgc ccatgttgtc gcgccagggg gtcagttcgg gcagggtgag gatttgctcg
     5221 agcgcggcgg catccaggct gacggggcgg gtggtggcgc gaatattgcc ctgaaagccc
     5281 tggtaacgga tatgcgaaac cagcaggcca tctgtggtcg aggcagcatc gggagcgttg
     5341 ccagtgacgg caaagaacgg cgccagttca atctgtttga gcgactcgag gctcttgcga
     5401 acggtggcaa cttcctcttc tggcgggcgg tctgagccgc cgcgcccggg gaagtgcttg
     5461 gcaatgaccg ccagacggta atcgctgccg gtgtgcaggc cattgatgta ggcgctgccc
     5521 atggccccca cccagtaggg gtcgccgcca aaggtgcggg tgcccaggtc gtcgctggtt
     5581 tccgagcgca gcacgtccag cacatcgagg gaggggccga ggtacaggtt gaagcccagg
     5641 gcctccagct cgcggcccat gacggtgccg acctgctgtg ccagggcggg cttccaggtg
     5701 gcgccaatag ccatctggtt gggcaggggc gtgaggtcat taaaaatcga atcgtagggg
     5761 gccaggtcgc cttcctgtga aacgccaaca aacagcggca cataaacacc gttggtttcg
     5821 gcagcgctgg tttgcagcgc ggtaatcaga ttttgagcgg ctacggcagt tccctcgggg
     5881 ccggtaaagt tatcgttatc ggcgctcaac accaccccgc cgacatgctg attaacgatc
     5941 aaatcgtaaa tttgagagtc tgtatcaacg ttcagacctt caaaggtgac cagaaagagc
     6001 tgtcccactt tttcctgggg ggtcatttgt gccagcagag cctgggcgcg ggcgcgggga
     6061 tcatcggtct gggccagggc acgagtggag ggaaagagcg ccgcagccag cagggcgatc
     6121 agagtgagca gcagcagcga gcgccggggc gtcatgatgc ggcggctcca taacgctgca
     6181 gcagcgcgag gtgggtttcg ggctgaatgt tgccgccggt tagtaccacc agggccgggc
     6241 gcgccatgac ctgcccgcat aataccgccg ccaacgccac cgcagccgag ccttcaatca
     6301 cctcgccgta gcgctgccag gcaaaggcca ccgcctgccc aatctgttct tcggagacca
     6361 gcaccaggtc atccaccagc gagcgcacca gcgggatggt cagggcatcg gcttcaacgg
     6421 cgccggaaag gccatcagcg aggctgtcgg tctcgatgac atcggcctgg gtgccgcgat
     6481 gaaacaagcc gtggaaaaag gccgaagcct gcgactgcac cccgatcaga cggggcgggt
     6541 gctgcaggcg gcgcagcgcc agcccaatgc cagagatcag accgccgccg cccaccggca
     6601 ccaggcagac ctgcgccggg aaggggctga gctgctcaag cagttcgagg cccagggtgg
     6661 cctgcccggc aatcacctga ccatcgttgt acggtgaaac ccaggcatag ccctgttcga
     6721 cagcaaacgc gtgggcggtg cgctcggcca gttcatagcc gccggggacg gtgcgaactt
     6781 cggcaccgag ggcgcgcatg gcagccagct tgagcggcgt ggcatggtct gaggcgaaaa
     6841 cgatcaccgg ggcggcgact tcttgagcag ccagggccac cccctgcccg tgattgcccg
     6901 ccgaggcgca aaccaggcca cgggcgcgtt cccagggctg cagggacagc actttattgg
     6961 ccgccccacg cagcttgaaa gagccggtgg tctgccggtt ttcccacttc agatagagat
     7021 ccagctcggc atcataagtc accggcgtga tgcgcacgcg gcccgccagg cgcgccgcgg
     7081 cggcatccag ccattcgggc ggaatcatgc catgttctcc agggtctggc gcgccagcaa
     7141 gggatcatca gtgaagatgc catccacgtc ggcggcaaac aggcggcgca tttcctgcgg
     7201 gtcgttcacg gtccagacgt ggacacggcg tttgagcgta tgttgatgct ggataaaatc
     7261 agcagtggca tcggtgaggt aaggatggac aatctccggg gccacgcggc ggcccagcca
     7321 ggcggtggtc ttgcggcggg cggcgccttg agagaacaga atggcgaccg gaacctgagg
     7381 cagcatctga cggatgcgca ccaggttgag gggttggaac gaggaaaaaa tcacctgatc
     7441 agcgttgttc agccgcttga tcagctctgc caccttgatg gtgagggcat ccagcgggga
     7501 ggtatagttg gtcagttcga cattgaccag gatattacgc cctacggtct caaagacttc
     7561 ttccagggtg gggatgcgct cgccgcgaaa gcggggggca aagaacgaac cggcgtccag
     7621 atcgcgcagg gccgccagcg gcaggtgatt gacaaaacct ttgccatcgg tggtgcgatt
     7681 gatggtgggg tcgtgaatga cgaccacctg accatcggcg ctgagcttgg catccagttc
     7741 aaccgccggg gcgccgtgct ccactgccag gcggaaagaa gcgagggtgt tttcgggggc
     7801 gtgcgcgcag gcgccgcggt gagcgaaaaa gacggggcgg ggtaaatcag tcagcaggtt
     7861 catagttcca caaagtaaga atcagccgca cgatccaata attcgcggct catagcaggt
     7921 tccaggttga ggtaacgagc aatatccagg atctggtcgc agagatcacg cgggtgtgag
     7981 gcgcgcagct tgcggttgcg tttgatgtaa tgctcctgga gcaggtaggc cagaccctga
     8041 tccgaatact ggacgccttt ttgaccggaa acgcgccgga aaatttcgcg gtattcttcg
     8101 taggtcgggt cgccaacttc aaccttgtgg cgcaggcggc gcaggaaggc ctcatccacc
     8161 agctcacgcg ggggcaggtt ggtggagaag atgaccagca cgtcaaaggg cacttcgatc
     8221 ttgcggccgg tgtgcagggc caggtaatca atccggtttt ccagcggcac aatccagcgg
     8281 ttgagcagat cgcgcgggcg aacctgctgg cggccaaagt cgtcaatcag caagatgccg
     8341 ccgttggctt tcatctggaa gggggcctcg tagaatttga gcgtgtcatc aaacaccagg
     8401 tccaggccag ccagggtcag ttcgccgcca gtgatgatga aggggcggcg gatgcgcatc
     8461 cagcgcgggt ctcggcgggc gccggtgcgc agcgagccgg tacccatggg ggtggcatct
     8521 tcttccggtg cgacctgatg attgaccgag tcatagagct tgatgacctg cccatccaca
     8581 ctgatggcgt aggggatgta catcaactgc gactggatca ggttgcccac ggcgcgggcc
     8641 acactggttt taccgttgcc aggcgggccg tacatgaaga tggaggtacc cgagttcacc
     8701 gccgggccca ggcgcgaata ggctttttca gagagcacca ggtgtgccag cacctgccgc
     8761 atgaggcggt tggtgatctg gaaacggccg cggctctggc ggcggatgga ttcgttatag
     8821 acggcaatcg gcacgggcgc cgggccagcg tactggctgc ggtcgagcgc ctcgcgagcg
     8881 cgggcgatgc cggcgccggt aatggcatag acataggcgc cttcccccag gcccatttgc
     8941 gaggtgcgga cttccagcag tttttcgcgc ttcagggctt cgagaagctg gtccacaata
     9001 ccggtaaaag gcagggcaat atcttcggcg atgcggaagc cggtcatgta gccctggaag
     9061 tacataattt tcaaaaccag gtcttgcagc cagagcggcg acaggcccgt atcttccatt
     9121 ctggagatgg gcggcggaac aaaggacatg gcgccagtgc cgacgggcgc attgccagcc
     9181 gggggctgtg cggggcgggg gggcatggta gtcataagac atcctccttc taagtcgtga
     9241 ggaacagcag gcacccccta ttatagcggt ttttgcgttc cgacggttgc ggtttggggg
     9301 ttgggttttt aggccaatag agtccggcgc gcgctacttg taatctttgg tttttcaatt
     9361 aca
//
LOCUS       DAOPVS010000072         9356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7396_length_9356_cov_57.985593, whole genome shotgun sequence.
ACCESSION   DAOPVS010000072 DAOPVS010000000
VERSION     DAOPVS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7396_length_9356_cov_57.985593"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKL11_07050"
     CDS             complement(<1..206)
                     /locus_tag="PKL11_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13398.1"
                     /translation="MNFTIATRSFVPSTPLPAHKPCLKVLGLGGGGSNAINRMIELGL
                     TGVDFIAANTDAQALNNSLAPVRL"
     gene            complement(211..1464)
                     /locus_tag="PKL11_07055"
     CDS             complement(211..1464)
                     /locus_tag="PKL11_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13399.1"
                     /translation="MAVTGLHYFPDTLHYRQSDLATWLPELHELGVGWLALRAESSRA
                     VPEFFLRSLIGAGITPILQFQLALHQPPQVEDLRPLLRAYSTWGVRYVVLFERPNQST
                     AWPEAEWLGEDLVERTLDRYLPLAEAALDAGLLPVFPPLEPGGSYWDLSFLSAALQAL
                     ERRRQTRLLETLTVSAYAHCYEHSLNWGLGGTVRWTGVRPYLTPEGSEDQRGFREYEW
                     IQAVVQKQLGGPRPMLLLEAGADAPAEVQLAAVRLANGQATPDPNNRSHPLAPLGAEV
                     LGCCFWLLSAAPDSPDADKAWYSADGKASELAQKVKALPRLMAPKQVPAGPQRPIRRY
                     VLLPGYEWGIADWHFEAIRPYVKKYRPTIGFSLAEAALADIVLVIGGEKDFSEEALNR
                     LRRAGCRVERINETGTTLATILAER"
     gene            1573..2490
                     /locus_tag="PKL11_07060"
     CDS             1573..2490
                     /locus_tag="PKL11_07060"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNZ13400.1"
                     /translation="MILVTGGTGFIGQALARQLVLNGHRVRVLLRPAPQSPNLPRHIP
                     VEVALCSLRDERGLRAAMKGVDAIFHLASAENASGRADLDEVDIGGAEAIAAAASQSG
                     VQRIFMLSQLGADRASAYPALRAKALAEGILQHGSVPVSIIRSASVYGPNDHFTIGLV
                     RLLRMSPGIFLLPGGGKTLLQPLWIEDLIACLLMALEDPSLVTRTIEIGGPEAISFKK
                     IVQDVMQASAVHRLLLPVELGYIRKISPWADHNPRFPLSIYWLDTLATDRTCSISTLP
                     HQFGLMPARFHQQLSYLNPPDKKAHSSTG"
     gene            2502..3950
                     /locus_tag="PKL11_07065"
     CDS             2502..3950
                     /locus_tag="PKL11_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNZ13401.1"
                     /translation="MSPQPSNSPSFCVHGHFYQPVREDPLTNEIPEEQGAAPYHDWNE
                     RIYAHCYAPNAELGNFERISFNIGPTLFTWLDLHHPETSEMIVTQERRNVERYGVGNA
                     MAQAYHHTILPLARREDKITEVMWGIADFENRFQHTPQGMWLPETAADLQTLEVLADC
                     GIQFTILAPWQAAVDSVDITHPYWVELPNNKRIVVFFYQPELSARISFDPGSTANADD
                     FYNHILLPQFYGAPTDSAPKFVMMASDGELYGHHQPFRDKFLSRLTQYSGDGPRPTFP
                     ALWLQQNQPTHTMAIREKTSWSCHHGVLRWSGQCGCTPHGEWKTHLRQALNRIAEVVD
                     AQYLAVVKPLLSDPWQLRHQYIRVLLGQLTLADLVELLVGRRLTEHQIIQLDLLLSAQ
                     HERQRMFTSCGWFFEDFDRIEPRNVVAYAAQAVWLTNLATGEDFSQKALGWLRPVESW
                     VSGLRADVVFSERLALTRKFFDRMQPSVWVPG"
     gene            complement(3957..4592)
                     /locus_tag="PKL11_07070"
     CDS             complement(3957..4592)
                     /locus_tag="PKL11_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13402.1"
                     /translation="MSVRILIADDHLIIRQGIRLILETGEDFELVGEAADGAEALRLC
                     RELHPDVVLMDLRMPGMDGITAIERLRVEMPEIGVVILTTFNEDDLMRRGLRAGAKGY
                     LLKDTSRERLFHAIQAAARGETLLAPEMLLKLLNGAENPPPTEGGMLKLSEREMEVLA
                     AVARGERSKEIALNLGISERTVKAHLASIYNKLGVDSRAAAIAIAAQRGLL"
     gene            complement(4589..5779)
                     /locus_tag="PKL11_07075"
     CDS             complement(4589..5779)
                     /locus_tag="PKL11_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNZ13403.1"
                     /translation="MKTRTPIINTERETRIFFWFMTVVLVAMSLWAIVAQPEMQQPWR
                     LGIFVLLMSAHILLHWILRVFAERPGWLIWYMLLQGGLAIALVYLSKDIGIAFAVSMA
                     LVGEAIGVYGITRRGFLAMAYYLILSLGFYIWVSGTTQVGWWLLGALPMVIFVVIYVE
                     MYTRQSNANERAQALLNELEAANRQLTNYAEQVEDLTIAAERQRMARELHDTLSQGLA
                     GLILQLEAANAYLESGRAEKAHQIIQNTMERARTTLADARRAIEDLREPSTPDCKDIL
                     QGEVARFSASTGVPCTLEIDIPQALPANLCDTMQRILAEGLANIAQHARAAHSQVIVS
                     AQNRTIQLEIRDDGQGFDPKTIPTGHYGLIGMRERIELAGGTLEIESRSGQGTSIIVR
                     MPIL"
     gene            5940..6905
                     /locus_tag="PKL11_07080"
     CDS             5940..6905
                     /locus_tag="PKL11_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13404.1"
                     /translation="MQPALHVHNLHKTFRNGHNLKAVDGVSFDIFPGEIFSLLGPNGA
                     GKTTTISMLSALLRPDLPSDGASEIAILGHSLQHEPRAAKALLGVVPQDIALYEDLSA
                     RENLTFWGKMYGLRGAALVQRVDEVLELIGLTERAKDRIGKYSGGMKRRVNIGVALLH
                     RPKVIYMDEPTVGIDPQSRRSILDGVMALKHQGMTVLYTTHYMEEAQELSDRIAIMDH
                     GKLIASGTHAELIQLVGQKARLILTVNVPTDRILQDWRSVPGVFSVVAADDTLTLLVD
                     DTNRVLPQLFEIAASRGVRITSVDIQEPDLESVFLHLTGRALRDA"
     gene            6918..8162
                     /locus_tag="PKL11_07085"
     CDS             6918..8162
                     /locus_tag="PKL11_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13405.1"
                     /translation="MKSLAIAFKDLTHSFRSTFAIIFMFGVPLMVTGLFYLMFGSLSA
                     DKSGFNLPPTKVVIVNLDAGDPQAGQLGQIMVAALQSEDFASLMDISLSPDSASARQL
                     VDTQQAGVAVIIPADFSTSFTDSQSATRLELYSDPTLTIGPGIVRSIISQFTDQFSGI
                     KITVALALKQAESGQIEYAQIESIIADYMAASQASADPQSLIESVTPTAAPQQNFMLT
                     LIGPIMAGMLIFYAFYTGVNTAMTILKESEDGTLSRLFTTPTSQTEILGGKWIAVGLT
                     VLVQVVTLLLVSRLIFQIEWGALPVVALVALAIVCSASAFGVFICSWLKNTRQGGIIF
                     GGVLTVTGMVGMMDVFTGSPGDPRFGFWPLITPQGWASRGLLASMNGAAVSQVLPYVL
                     ALVAMSIIFFIIGVLRFQKRYA"
     gene            8208..>9356
                     /locus_tag="PKL11_07090"
     CDS             8208..>9356
                     /locus_tag="PKL11_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13406.1"
                     /translation="MLPILDLASKDLLQMLRDRKTFLFLLLMPVAFTLLFGFIFNGTA
                     QTDTDARLPVGLLNQDQAAPLSLELEALLSSSNVIRLEFYEDVATLEEAVIDQSLAAA
                     VIIPAGYGDSLQSNLPLKLTVIADPAVPAGLSAQTEISVAASRLNRAIHTAQVIASQG
                     RASFDAALHDALTAWQDPPVHLALTQSQTTLAEPGEPPAPANNFAHSSPGMILQFAIA
                     GLLTCAQVIVSERKNRCLQRLLTTATSRVQILLGHYLSIFLLIFSQFAILILFGQFVL
                     KLDYFAHPLATLLIALTAALCISALGLLIGVLSKSEEQAVTYALICMFILAGVGGAWV
                     PLEFTGSTFQTIGHLTPLAWAMDGFKNILVRGLGLTSVLLPAAALLGYA"
BASE COUNT         1908 a         2930 c         2326 g         2192 t
ORIGIN      
        1 tgcaggcgca ccggcgccag gctgttattc agcgcctgtg catcagtatt tgcagcaatg
       61 aaatcaacgc cagtcagtcc aagttcaatc atccggttga tggcgtttga gccgccgccg
      121 cccagcccaa gcaccttcag acagggcttg tgggctggca gtggcgtgct gggtacaaaa
      181 gaacgggtgg caattgtgaa gttcatggga tcacctctca gccagtattg ttgcaagagt
      241 cgtgccggtc tcgttgatcc gttcaacccg gcagcccgcc cggcgcaggc ggttcagcgc
      301 ctcttccgaa aaatctttct cgccgccaat cactaaaaca atgtctgcca gcgcggcttc
      361 agccagcgaa aaaccgatcg ttgggcgata ttttttgaca tagggccgaa tggcttcaaa
      421 atgccagtcc gcgatgcccc attcatatcc cggcagcaga acatagcgcc tgatggggcg
      481 ctgcggaccg gcaggaacct gcttcggagc catcagccgc ggcagcgcct taactttctg
      541 tgccagttcg cttgcttttc cgtctgccga ataccaggcc ttatccgcat ctggcgaatc
      601 gggcgccgcg ctcagcagcc agaaacagca gcccagcacc tccgcaccca gcggcgccag
      661 cgggtgagat cgattgttgg ggtccggggt ggcctgccca tttgccagac gcacggccgc
      721 cagttgaacc tctgccgggg catccgcgcc ggcttccagc agcagcatgg gccgcggccc
      781 gcccagttgc ttctgtacaa ccgcctgaat ccattcatat tcccgaaatc cgcgctggtc
      841 ttcggagccc tccggggtca ggtaggggcg cacccctgtc cagcgaactg tgccgcccag
      901 cccccaattc aatgaatgct cataacaatg cgcataggca gaaaccgtca gcgtttccag
      961 cagcctggtt tgacgccgcc gttccagcgc ctgcagcgcc gcgcttaaaa agctgaggtc
     1021 ccagtaactg cctcccggct ccagcggtgg aaaaacaggc agcagcccgg catccagtgc
     1081 ggcttcagcc agcggcaaat agcggtccag ggtgcgctca accagatctt cccccagcca
     1141 ttcagcctca ggccaggctg tactttgatt gggcctttca aacagcacaa cgtaccgcac
     1201 cccccaggtt gaataagcgc gcagcagggg gcgcaagtcc tcaacctggg gcggctgatg
     1261 cagcgccagt tggaactgta agatgggcgt gatacccgca ccaatcaggc tgcgcagaaa
     1321 aaactcaggc actgcccggc tgctttctgc ccgcagcgcc agccagccta cccccagctc
     1381 gtggagttcg ggcagccagg tcgccaggtc agactggcgg taatgcaggg tatccggaaa
     1441 ataatgcaga ccagtgacag ccatagacgc ttccagaaga aagagtgacg tgctgccttc
     1501 aacggagcaa gaaatatgcc agtctccagg ataacgcctc ttaaaaccca aaagacagta
     1561 taatttccca tcatgatttt ggttaccggt ggcaccggtt ttattggtca ggcgcttgct
     1621 cgccagttgg tcctcaatgg ccatcgggtt cgggtcttac tgcgccctgc tcctcagtcg
     1681 ccgaatctgc cgcggcacat ccccgttgag gtggcgcttt gctcactgcg cgatgagcgc
     1741 ggcctgcgcg ccgccatgaa aggcgtggat gccatcttcc accttgccag cgccgaaaac
     1801 gcctccggac gggcagacct cgacgaggtg gatatcggtg gcgccgaagc cattgccgca
     1861 gctgcgtccc agtcgggcgt tcagcgtatt ttcatgctca gccagttggg cgcagaccgc
     1921 gcctctgcct acccggcctt gcgagccaaa gcattggccg aaggcattct ccagcacggc
     1981 tccgtccccg tcagtatcat tcgttcggcc tcggtttatg gccccaacga tcacttcacg
     2041 attgggctgg ttcgcttgct gcgcatgagt ccgggtattt tcctgcttcc cggcggtggt
     2101 aagaccctgc tccaacctct ctggattgaa gacctcatcg cctgcctgct gatggcgctt
     2161 gaagatccat ctctcgtcac tcggaccatt gaaattggtg ggcctgaggc catttctttt
     2221 aagaaaattg tacaggatgt catgcaggcc tctgccgtgc accgcctgct gttgcccgtt
     2281 gaattgggtt acatcagaaa aatctcaccc tgggctgatc acaacccccg cttcccgctt
     2341 tcaatttatt ggctcgacac ccttgccaca gaccgcacct gctcgatcag caccctgccg
     2401 catcaatttg gcctgatgcc cgctcgcttc catcagcagt tgtcctatct taatccaccg
     2461 gataaaaaag ctcactccag caccggttag aaagaatccc tatgtcgccc caaccttcca
     2521 attctcccag tttttgtgtt cacgggcatt tttatcaacc cgttcgtgaa gaccccctga
     2581 ccaatgagat ccccgaagag cagggcgcag ccccctacca tgactggaac gagcgcattt
     2641 acgcccattg ctatgcccca aatgccgaac tgggcaactt tgagcgcatc agctttaaca
     2701 tcggcccaac cctgttcacc tggctggatc tgcaccaccc cgaaaccagc gaaatgattg
     2761 ttacccagga gcgccgcaat gtagagcgtt atggtgtggg caatgccatg gctcaggcct
     2821 accaccatac cattctccct ctcgcccggc gagaagataa aatcaccgag gtgatgtggg
     2881 gcatcgctga ttttgagaat cgcttccagc atacgcccca gggcatgtgg ctgccagaaa
     2941 ccgctgccga tctgcaaacg ctggaagttt tggccgattg cggcatccag ttcaccattc
     3001 tggcaccctg gcaggcggcc gtggattcgg tagatatcac ccatccctac tgggttgaac
     3061 tgcccaacaa caaacgtatt gtcgtcttct tttaccaacc cgaactcagc gcccgcatta
     3121 gcttcgatcc tggctcaacc gccaatgccg atgacttcta taaccacatt ctcttgcccc
     3181 agttttatgg cgccccaacc gattccgccc cgaagtttgt catgatggct tccgatggcg
     3241 aactctacgg ccaccatcaa cccttccgcg ataaattcct ctcccgcctc acccagtaca
     3301 gcggtgatgg cccccggccg acctttccgg cgctctggct tcaacagaac caacccaccc
     3361 ataccatggc cattcgtgaa aaaacctcct ggagctgcca ccacggtgtt ctccgctgga
     3421 gcggacagtg tggctgcacc ccccacggcg aatggaaaac ccatctgcgc caggccctca
     3481 accggattgc cgaagtggta gatgcccagt accttgctgt ggtcaagccg ctcctgtccg
     3541 atccctggca gttacgccac cagtacatcc gggtcttgct cggccagctt acacttgccg
     3601 acctggttga gttgttggtg ggccgccggc tcacagagca ccagatcatc caattggatc
     3661 tcctcctcag cgcccagcat gagcgccagc gcatgttcac ctcctgtggc tggttctttg
     3721 aagatttcga ccgcatcgaa ccgcgcaatg tggtggccta tgccgctcag gcagtctggc
     3781 tcaccaacct ggctaccggt gaagatttct ctcaaaaagc cctgggctgg ctgcgccctg
     3841 tcgaatcctg ggtcagcggc ctgcgcgccg acgtagtatt ctccgagcgc ctggccctta
     3901 cccgcaaatt ctttgaccgg atgcagccct cggtctgggt tcctggctga gccgggctat
     3961 aacaacccgc gttgtgcggc aatcgcaatc gccgccgccc gtgaatcaac ccccagtttg
     4021 ttgtagatac tggccaggtg tgccttgacg gtccgctcgc tgatgcccag gttgagcgca
     4081 atctccttgc tgcgctcccc gcgcgccacg gctgccaata cttccatctc gcgctcactc
     4141 aacttaagca ttcccccttc agttgggggc ggattttctg ccccattcag cagctttagc
     4201 agcatctctg gcgccagcag tgtctctcct cgcgcggctg cttgaatggc atggaataac
     4261 cgttcccggc tggtatcttt gagcaggtaa cccttggccc cggcgcgcaa gccgcgccgc
     4321 ataagatcat cctcgttgaa agtggttaaa atcaccaccc caatttccgg catttcgacc
     4381 cgcagacgtt caatcgccgt aatgccatcc atgccgggca tgcgcaggtc catcaacacc
     4441 acatccgggt gcagttcacg gcacagccgc agcgcttctg cgccatcggc agcttctccc
     4501 accagttcaa aatcttcgcc ggtttccaga atcaggcgaa tgccttgccg gatgattaaa
     4561 tggtcgtctg caatcagaat tcgaacactc ataggattgg catcctgacg atgatggacg
     4621 ttccctgtcc ggagcggctt tcaatttcca gcgtcccgcc ggccaactcg atccgttctc
     4681 gcatgccaat taatccataa tgcccggtgg gtatggtctt gggatcaaaa ccttgcccgt
     4741 cgtcacgaat ttcaagctga atcgttctgt tttgggccga aacaatcacc tggctgtgtg
     4801 cagcccgcgc atgttgggca atatttgcca acccttcagc caggatgcgt tgcatggtgt
     4861 cacaaaggtt ggctggcagg gcttgtggta tgtcaatctc caatgtgcag ggaaccccgg
     4921 tgctggcact gaagcgcgcc acctctcctt gcagaatatc cttgcagtct ggcgtggagg
     4981 gttctcgcag atcttcaatc gcccggcggg catctgccag cgttgtgcgc gcccgttcca
     5041 tcgtattttg aataatctga tgggcttttt ctgctcgccc gctttccaga tacgcattag
     5101 cagcttcaag ctgtaaaatc aatcccgcca gcccctgcga aagtgtatcg tgcagttcac
     5161 gcgccatgcg ctgccgctcg gcagcaatgg ttaaatcttc cacctgttca gcatagtttg
     5221 taagctgtcg gttagccgct tcaagctcat tcaataaagc ttgggcgcgc tcattcgcat
     5281 ttgattggcg cgtatacatc tccacataaa ttaccacaaa aatgaccatc ggcagagcgc
     5341 cgagcagcca ccagccaacc tgtgttgttc ccgataccca gatgtaaaac cccagcgaca
     5401 atatcagata gtaagccatg gccaggaatc cacgccgggt aatcccataa accccaatcg
     5461 cctcgcccac cagcgccatt gaaacggcaa acgcaatccc aatatcttta gagagatata
     5521 ccagggcaat tgccagcccg ccttgcagca gcatatacca gatcagccag ccagggcgct
     5581 cagcaaacac cctcaagatc caatgcagca aaatatgggc gctcattaac aaaacaaaga
     5641 ttcccagccg ccagggctgc tgcatctctg gttgagccac gatcgcccac agactcatgg
     5701 cgaccaaaac gacggtcata aaccagaaaa agatgcgtgt ctctcgttct gtattgataa
     5761 tgggagttct tgttttcata tctgctttta ttgtatgtct tcttgccttt ttttaccacc
     5821 gcccaaacgg acattgtcca aaaggacagt ccaacccggc ccgattgccc gatgtaattt
     5881 tctgtcagac tgactaaact tcaatctagt cagcttattt ctcgaaaacg gagtctctca
     5941 tgcaaccagc acttcatgtt cacaatctgc ataaaacctt tcgcaatggt cacaacctta
     6001 aagctgtaga tggtgtcagt tttgatatct tccccggtga gatttttagc ttgctgggcc
     6061 ccaatggcgc tggcaaaacg accaccattt ccatgctctc tgccctctta cgccccgacc
     6121 tcccatcgga cggagccagt gaaattgcaa tcctgggcca ttcactgcag cacgaacctc
     6181 gggctgccaa ggctttgctt ggcgtggtgc cccaggatat cgccctttac gaagacctct
     6241 ctgcgcgtga aaacctcacc ttctggggca aaatgtatgg cctgcgcggt gctgccctgg
     6301 tccagcgggt tgacgaagtg ctggaattaa tcggcctgac cgagcgcgcc aaggaccgga
     6361 ttggtaagta ctcgggtggc atgaagcgcc gtgtcaatat cggtgtagct ttgctccatc
     6421 ggcccaaagt aatttatatg gacgagccaa cagtcggcat cgatccgcaa agccgccgca
     6481 gtattctgga tggcgtgatg gccctcaagc atcagggcat gaccgtgctc tacaccacgc
     6541 attacatgga agaagcccag gaactttctg atcgcatagc catcatggat catggcaaat
     6601 tgattgccag cggcacccac gcagagttaa tccagcttgt gggccagaaa gcacgtctaa
     6661 tcctgaccgt caatgtccca actgatcgca tcttgcaaga ctggcgatct gttcccgggg
     6721 ttttcagtgt ggttgcagcc gatgacacgt tgaccttgtt ggtggatgat accaaccgcg
     6781 tgctcccgca gctttttgaa attgctgcct ctcgcggtgt tcgcattacc tcagtagata
     6841 tccaggaacc cgacctggaa tcggtgtttc tccatctcac cggccgcgcc ctgcgtgacg
     6901 cctgaacgga gcccctcatg aaatcccttg ccattgcctt caaagacctg acgcattcat
     6961 tccgctccac gtttgccatc attttcatgt ttggcgttcc cctgatggtc accgggcttt
     7021 tctacctcat gtttggcagt ctatctgctg acaagtccgg tttcaatctg ccccccacca
     7081 aagtagtgat tgtcaatctc gatgctggcg atcctcaggc cgggcagttg gggcaaatta
     7141 tggtagcagc cctccagagt gaggattttg ccagcttgat ggatatctcc ctgtctccgg
     7201 acagcgcttc tgcccgccag cttgtggata ctcagcaggc tggggtagcc gtgatcatcc
     7261 ccgcagactt ctccacctcg ttcaccgact cccaatcggc aacccggctt gaattgtatt
     7321 ctgatcctac cctgaccatc ggccctggga ttgttcgttc aatcatcagt caatttacag
     7381 atcagttctc cggcatcaaa atcacggtag ccctcgccct caaacaggct gaatctggtc
     7441 agatcgagta cgctcaaatt gagtcaatca tcgccgacta catggcagca tctcaggcca
     7501 gcgccgatcc gcaatcgctg atcgaatcag tcaccccaac ggctgccccg cagcagaact
     7561 tcatgctgac tttgattggc ccaatcatgg ccggcatgtt gatcttctat gccttttaca
     7621 ccggtgtcaa taccgccatg acaatcctca aagagagcga agatggcacc ttgagccgtc
     7681 tctttaccac gcccacctcg caaaccgaaa tccttggcgg caaatggatt gcagtcggct
     7741 tgacggtgct ggtgcaggtg gttacccttc tgctcgtttc ccgcctgatc tttcaaattg
     7801 aatggggcgc cctcccggtg gtcgcgctgg tggcgctcgc cattgtctgc tcggcctcgg
     7861 cttttggtgt tttcatttgt tcgtggctca aaaacacccg gcagggaggc attatctttg
     7921 gcggcgtgct caccgtcact ggcatggttg gcatgatgga tgtctttacc ggcagtcctg
     7981 gtgacccccg gtttggattt tggcctttga tcacgcccca gggctgggcc tcacgcggcc
     8041 tgcttgcctc aatgaacggc gctgcggtct cccaggttct gccctatgtg ctggcgctgg
     8101 tcgcgatgag catcatcttt ttcatcatcg gcgtactgcg ctttcaaaag cgctatgcct
     8161 gatcccactt accccgagcc acatctcgat aaataggagc ccgtcacatg ttacccattc
     8221 ttgaccttgc gtctaaagat ttgttacaaa tgctgcgcga tcgtaaaacc tttttgttct
     8281 tgctgctgat gcctgtcgct tttacattgc tgtttggctt tatttttaac ggcacagcgc
     8341 aaacagatac cgatgcccgt ctgcccgtcg gattgttgaa tcaggaccag gccgcaccgc
     8401 tcagtctgga attagaagcc ttgctctcgt cttcaaacgt aatccgactg gaattctatg
     8461 aggatgttgc caccctggaa gaagctgtca ttgaccagtc tctggccgcg gctgtcatca
     8521 ttcctgccgg gtatggcgat tcgctccaga gtaatctccc tctcaaatta acggtcattg
     8581 ccgaccccgc cgtccctgcc ggtctcagcg ctcaaacaga aatcagcgtg gctgccagcc
     8641 gcctgaaccg cgccatccat acggctcaag tgattgcatc acagggccgc gccagttttg
     8701 atgccgcgct tcacgatgcc cttacagcct ggcaggatcc ccccgtgcat ctggctttaa
     8761 cccaatctca aaccaccctt gcagaacccg gtgagcctcc ggctcctgcc aataattttg
     8821 cccatagttc tccgggcatg atcctgcagt tcgcaattgc cggtttgctc acctgcgccc
     8881 aggtcatcgt ttcagaacgt aaaaaccgct gcctccagcg cttgctgacc accgccacca
     8941 gccgggtaca aattctcttg ggacattacc tctcaatctt tctcctgatc ttttcccagt
     9001 tcgccatcct gattctcttt gggcagtttg ttctcaagct ggattatttc gctcacccct
     9061 tagccaccct cttgattgcc ctcacggcgg ccctgtgcat ctccgctctg ggtctgctga
     9121 ttggggtgct gtccaaatca gaggagcagg ccgtgaccta tgctttgatt tgcatgttta
     9181 ttcttgctgg cgtgggcgga gcctgggtgc cgcttgaatt tactggctca accttccaga
     9241 caatcggtca cctcaccccc ctggcctggg cgatggatgg tttcaagaat atcctcgtcc
     9301 gcggtctggg tttgacttca gttctgctcc cggctgcggc cttactgggt tatgcc
//
LOCUS       DAOPVS010000073         9293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7466_length_9293_cov_46.320199, whole genome shotgun sequence.
ACCESSION   DAOPVS010000073 DAOPVS010000000
VERSION     DAOPVS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_9293_cov_46.320199"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PKL11_07095"
     CDS             <1..1083
                     /locus_tag="PKL11_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNZ13407.1"
                     /translation="VSAEYRFGEQLTITAQLTADQPIAQVVLFLQQADRPTRAKELTP
                     GANGELLAQFNLLEQPLRPFARVDYWFEVTPAEGDVYHSPVFSLDYEDNRQKWQRLED
                     NGLQVAWLEGDVTFGQQIINTVAASLERNQQFINSEAGQPLKIYVYPTAAALQEALKL
                     EGANWVAGHASPELGTVLLSIAPGSTQGMEMERQIPHELMHIQLYEKTGENYGRIPIW
                     LSEGLASLAELYPNPDYSRVLDQASANGMLLPIETLCSNFPMDASGAYLAYAESASFV
                     KHQYELYGTSGLDRLISAYASGQGCVEGFETALGTTLNQAEVNWKQEVLGVDTVNMVL
                     RTLGPYLLLLALVIGVPLGAVLIRRK"
     gene            1094..1387
                     /locus_tag="PKL11_07100"
     CDS             1094..1387
                     /locus_tag="PKL11_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNZ13408.1"
                     /translation="MKNEGLIGLHAVVQGVVQGVGFRAFVIAEAQALGVTGWVRNTWD
                     GKVEVQAEGTRTMLEELLSKLRRGPRNAVVYAVTVDWGEAGGEFSGFSMRRSV"
     gene            complement(1466..2014)
                     /locus_tag="PKL11_07105"
     CDS             complement(1466..2014)
                     /locus_tag="PKL11_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13409.1"
                     /translation="MKKWLWMLAGFGVAVVLVVAGLAIVTSVNANAVLPQIGDGPGGR
                     HGGRGGMMGGGGMMGGTGDTDRGLLSDVMHTAMLSVFADRLATTPTDLQARLDAGESL
                     YDIAEAAGVTQEEFVTLRDTARTQALADAVSQGLITQEQADQMAARHAGGGMHSGRGG
                     RMGFGGTCNCDCTGNTETETDN"
     gene            complement(2125..4179)
                     /gene="uvrB"
                     /locus_tag="PKL11_07110"
     CDS             complement(2125..4179)
                     /gene="uvrB"
                     /locus_tag="PKL11_07110"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNZ13410.1"
                     /translation="MEFRLQAPYQPTGDQPEAIAQLVQNLRQGSRHQVLLGATGTGKT
                     FAIANIIQQLQMPALIMAHNKTLAAQLYAEFADFFPENAVSYFVSYYDYYQPEAYVPQ
                     RDLYIEKETDINEEIDRLRLAATASLMSRRDVIIVASVSCIYGLGNPEAYGHGAIHLQ
                     QGAIYRRNALLRQLIEGQYQRNDFDFHAGTFRVRGDTLEIFPAYEEKQAYRVTFFGDE
                     VERMMAVNPLTGEILSEPAEITIYPAKHYIAQEDRLKSSIVNIEQELEERLAQFKREG
                     KLLEAQRLEQRCRYDLEMLREVGYCSGIENYSRQLDQRAPGSPPWTLIDYLPSDYLLV
                     IDESHMTIPQIRGMYNGDRSRKGTLVEYGFRLPSAMDNRPLTFDEFEQHMGYTIYTSA
                     TPGPYELQRSDHVVEQIIRPTGLVDPEVEVRPTAGQIDDLLKEIRRRVEVGERVLVTT
                     LTKRMAEDLADYLMEAGIKVHYLHSEVETIERIGILRDLRMGVFDVVVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSATALIQTIGRAARHLHGRVILYADKMTDSMRFAIDETN
                     RRRAKQTAYNEAHGIEPISIHKAIRDLTERLSPNAVPVTAEARAEYQANERARSARMP
                     TRDLQRLIVELEKEMKQAARDLEFERAAGLRDQIYELRGVLAEESNLPPWQKARLLAG
                     EE"
     gene            complement(4262..5497)
                     /locus_tag="PKL11_07115"
     CDS             complement(4262..5497)
                     /locus_tag="PKL11_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ13411.1"
                     /translation="MSNKQEAPKKINLVFRDHLYQLRGFMDKFINNQEFMVELFDNAE
                     LAERAGEIGAAILEARSLRLTDIAVKMEGESAAGYKRIQRFLGKADPRTALWRLFQED
                     AEFVIGDPTEIERPHAWKTEYVGKLKDGKTRGFWALILATPYRGRAIPCGMVTYSSRT
                     IAQGLDSRNLNHFRAFEGLKDMLGGRPLVLDREFSYLELMLNLFEEQINWVIRLNLRS
                     HPPKFYDDDGKEVALTISPGETVVLNHIWYMGKVCMNVVGTWKKGLSEPMWVMTNLEA
                     KRGLQIYFTRMKIEETYRDLKSLLGLTKLMNKQQVYMEKMVGLLLLTFTIGLLVGEEL
                     RDLLYGEPIAEEEPIEDYERIPGSPSLKKGKKWKRYSGLFVLLKQKWSLSDTQRAAVL
                     HAALTTFRCLVYPNVRTFV"
     gene            complement(5528..6907)
                     /locus_tag="PKL11_07120"
     CDS             complement(5528..6907)
                     /locus_tag="PKL11_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ13412.1"
                     /translation="MRTKFILSLVLVALAAIASVVIFVRLDSRQQIQTYMLRGGFEGL
                     EETVSDLEAYYAEHGSWQGADSLLSVGAGPAEGHGPGGNGGHGGGSQRLRLADSQGLV
                     LIDASGQTEGSVLSSDDLAGGIVLTGPQGAAVGYLLPSTGSFYRQGDELPLIERLNDA
                     ALRAGLVGVGIALLMALALSAGLIRPIRQLTRAAHAMSAGDLSQRVAIRGRDELSDLG
                     RTFNHMAASLQQSEERRRSMTADIAHELRTPLAVQRAQLEALLDGVNPMTPENLNAIL
                     AQTDLLTRLVGDLRTLALAEAGELPVERVPVDLVALTRRVVNRFDAPASARNVELTIE
                     PSSPLQALGDPDRIEQILGNLLSNALRYTPDAGRITIALASTGLLTVHDSGPGIAPEA
                     LPHIFERFYRAERSRSRDEGGTGLGLAIARQLARAMGGDVTAANHPSGGAVFTVTLPL
                     STSRSSIAR"
     gene            complement(6916..7632)
                     /locus_tag="PKL11_07125"
     CDS             complement(6916..7632)
                     /locus_tag="PKL11_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13413.1"
                     /translation="MTKILIVEDEPELAKILQAYLEKAGFEVLLSARGDTGLQTWERE
                     HPDLVLLDLNLPGLDGLDVAHAIRRKSETPLIMVTARAEETDRLIGLELGADDYIVKP
                     FSPREVVARVRAVLRRSERGAVTETQNNIMRIANIEIDLDAHSVRRDDQPIDLTPSEF
                     NLLAALASQPGRVFSRLQLLEAAQGVAYEGYERSIDAHIKNLRAKLEPEPRIPRYIET
                     VFGVGYRFRKPDNPSPDLEP"
     assembly_gap    7721..7820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7821..>9293)
                     /locus_tag="PKL11_07130"
     CDS             complement(7821..>9293)
                     /locus_tag="PKL11_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ13414.1"
                     /translation="VLAQQHYETFRERLAAFPVTVEMLSRFRTPREQSEILLKLALGE
                     VDIIIGTHRLLQPDVEFKDLGLAIVDEEQRFGVTHKEHFKKLRTEIDVLTLTATPIPR
                     TLYMALTGVRDISNINTPPEERLPIITHIGPYSPRLVRQAILRELERGGQVFFVHNRV
                     QSIHAMERHLNALVPEARVGIGHGQMHESDLARVMHEFTRGEIDVLLCTSIIESGLDI
                     PNANTLIVDRGDAFGLAQLYQLRGRVGRGAQRAYAYFFRHRRKPPTPEGQERLEVIAE
                     NTQLGAGYSIAMRDLEMRGAGELLGMRQHGYIASVGFHLYTRLLAQAVRQMRAVSGLP
                     APDDRMLSAPELRMAITVDLPLSVGIPLDYVPDQQQRLNLYRRIADLQSEDEVDALRE
                     EFIDRFGALPESVENLFFQMRVKLRAYLAGLNLVAVEGDQMVLRFPLLPEGSPPRQLL
                     PLGPTARAGKNAYWLSMSSPDWRTDLLEALSAIVQNEFSK"
BASE COUNT         2030 a         2418 c         2816 g         1929 t
ORIGIN      
        1 gtatcagcag aataccgctt cggcgagcag ttgacaatta cagcccaatt gactgcagac
       61 cagccgattg cgcaagtggt gttgttttta cagcaagcag atcggccaac ccgggctaaa
      121 gaactgacgc ctggagccaa cggcgaactg ctggcgcagt ttaatttgtt ggaacagccc
      181 ctgcgcccgt ttgcacgagt ggattactgg tttgaggtga ccccggctga gggcgatgtg
      241 tatcactcgc cagtgttttc gctggattat gaagataacc gtcaaaagtg gcagcgcctg
      301 gaagataatg ggctgcaggt ggcctggctg gaaggggatg tgacctttgg gcagcaaatt
      361 atcaatacgg tggcagccag tttggaacgc aatcagcaat tcattaacag cgaagccggc
      421 cagccgctca aaatttatgt gtacccgacg gcggccgcgc tgcaagaggc gttgaagcta
      481 gagggggcca attgggttgc cggacatgcc agcccggagc ttgggacagt gctgctctcc
      541 attgcgccgg gcagcacgca gggcatggag atggagcggc agattccgca tgagttgatg
      601 catattcagc tctatgagaa aaccggagag aattacgggc ggattccgat ctggctgtca
      661 gaaggattgg cttcactggc ggaactctat cccaacccgg attacagccg ggtgctggat
      721 caggcttcgg caaacggcat gctgctaccc attgaaacgc tgtgcagcaa ttttcccatg
      781 gatgccagcg gggcctacct ggcttatgct gaatcggcct cgtttgtaaa acatcaatac
      841 gagctgtatg gcaccagcgg cctggaccgg ttgatcagcg catacgccag cggacaaggg
      901 tgtgttgaag gttttgagac agccctgggc acaacgctga accaggcaga agtgaattgg
      961 aaacaagaag tactgggggt ggatacagtg aacatggtct tgagaacgct ggggccatat
     1021 ctgctgctgc tagctctggt aatcggggtg ccgctggggg cggtcttgat ccggcgaaag
     1081 tgaggcgctg ccgatgaaaa atgagggctt gatagggttg cacgccgtgg ttcagggcgt
     1141 tgttcagggg gtggggttcc gggcttttgt gattgccgaa gcacaggcgt tgggtgtgac
     1201 tggctgggtg cggaacacct gggatggcaa ggtggaggtg caggctgaag gaacccgcac
     1261 aatgctggag gaattattga gcaaactgcg gcgcgggccg cgcaacgcgg tggtttatgc
     1321 tgtgacagtg gactggggag aagccggggg agaattttcg gggttttcga tgcggcggtc
     1381 tgtgtgaaat gacccggcta ttaaaataaa atcgggctga tggacgagcc atcaacccga
     1441 ctgtttgtgg tttcaaccgt agaaattagt tatcggtttc agtttcagta ttaccggtgc
     1501 agtcgcagtt gcaggttcca ccaaagccca tccggccgcc gcgacccgag tgcatgccac
     1561 cgccagcgtg gcgggcagcc atttgatcgg cctgctcctg ggtgatcaaa ccctgagaga
     1621 cggcatcggc cagggcctgg gtgcgggctg tgtctcggag ggtaacaaat tcttcctggg
     1681 tgacgccagc cgcctcagca atatcgtaga gcgattcgcc cgcatcgagc cgggcttgca
     1741 ggtctgtggg agtggtggca aggcgatctg caaagacgga cagcatggca gtgtgcatca
     1801 catcggagag caaaccgcgg tctgtatcac ccgtgccgcc catcatacca ccgccaccca
     1861 tcatgccgcc gcggccgcca tggcggccac cggggccatc gccaatttgc gggagaaccg
     1921 cattggcatt aaccgaggtc acaattgcca gaccagctac cactaagaca acagctacac
     1981 caaaaccggc taacatccag agccattttt tcattgtttt actcctgatc tcaatttccg
     2041 gttgaacgac cggattgatt ataggatacc gtaaagttgt gaagaagtta tgaagaaatc
     2101 gtaagcttgt tgtaaagatt ggggctattc ttcaccggca agcaggcggg ccttttgcca
     2161 ggggggcaaa ttggattcct cagccaggac gccgcgtaac tcataaatct ggtcccgcag
     2221 accagcagca cgctcaaatt cgaggtcacg ggcagcctgt ttcatttcct tttcaagctc
     2281 gacaatcagg cgctgcagat cgcgggtggg catgcgggca gaacgagcgc gttcgttggc
     2341 ctggtattcg gcgcgggcct cggcagtcac tggtacagca ttgggggaga gccgctcagt
     2401 caaatcgcgg atcgctttgt gaatgctgat cggttcgatg ccgtgggcct cgttataagc
     2461 cgtttgcttg gcgcggcggc ggttggtctc atcaatggca aagcgcatgg aatccgtcat
     2521 tttgtcagca tacagaatga cgcggccgtg caggtgacgg gcggcgcggc cgatggtctg
     2581 gataagggct gtggctgagc ggaggaaacc ctccttgtcg gcatccagga tggccaccag
     2641 ggagacttcg gggaggtcga gcccttcacg cagcaggttg atgcccacaa ccacatcaaa
     2701 gacgcccatg cgcaggtcac gcagaatgcc gatgcgttcg atcgtctcaa cttcggaatg
     2761 gaggtaatgc accttgatgc cagcttccat caggtaatcg gcgaggtctt cagccatgcg
     2821 tttggtcaga gtggtgacga ggacgcgctc ccccacttct acgcgacggc gaatttcttt
     2881 gagcaggtca tcaatctgac cagcggttgg gcggacttct acctcggggt caaccagacc
     2941 ggtcgggcgg atgatctgct cgacgacatg gtcggagcgc tgcagttcat aggggccagg
     3001 ggtggcgctg gtatagatgg tgtagcccat gtgctgctca aactcatcga aagtcaacgg
     3061 gcggttatcc atggcagagg ggaggcggaa accgtattca accagggtgc ctttacgcga
     3121 gcggtcaccg ttgtacatcc cccgaatctg ggggatggtc atatgggact cgtcaatgac
     3181 gagcaggtaa tcactgggga ggtaatcaat cagcgtccag ggtggagacc cgggggcacg
     3241 ctggtccaac tggcgggagt agttttcgat gccggagcag taacccactt cgcggagcat
     3301 ttcaaggtca taacgacagc gctgttccag gcgctgcgct tcgagcagct tgccttcgcg
     3361 cttgaattga gccaggcgtt cttccaattc ctgttcgatg ttgacaatgg aggatttcag
     3421 gcgatcttcc tgggcgatat agtgcttggc ggggtaaatg gtgatttcgg ctggctcgga
     3481 caggatttca cctgtcaggg ggttgacggc catcatgcgt tcaacttcat caccaaagaa
     3541 tgtgacccgg taggcctgct tctcttcata agcggggaag atttcaagag tatcgccgcg
     3601 cacgcgaaac gtgccggcat ggaagtcaaa gtcattgcgc tgatactggc cctcaatcag
     3661 ctgacggagg agggcattgc ggcggtagat ggcgccctgc tgtaaatgga tggcgccatg
     3721 accgtaggct tcggggttgc ccaggccata gatgcacgac acagaggcca caataatgac
     3781 atcgcggcga gacatgagcg aggcagtcgc cgccagacgc aggcgatcaa tctcctcgtt
     3841 gatgtcagtc tctttctcga tatagaggtc acgctgggga acgtaggctt cgggctgata
     3901 ataatcgtag tacgagacga agtaagagac cgcgttctcg gggaaaaaat cggcaaattc
     3961 ggcatagagc tgagctgcca gagtcttatt atgcgccata atcagcgcgg gcatctgaag
     4021 ctgctggatg atattggcaa tggcaaaggt cttgccggtg ccggttgcgc caagcagcac
     4081 ctgatggcgg ctgccctggc gcagattctg aaccaactgg gcaatcgctt ctggctgatc
     4141 gccagtaggc tgataggggg cctgtagacg aaactccatg agacacctgc cttctaagaa
     4201 ttggtcaagt gttctattat aatctcagat tggatgaaag gcaattgtca gattctgagg
     4261 ttcagacaaa agttcggaca tttgggtaaa ccaagcaacg aaaggtagtc agggcggcat
     4321 gcagaaccgc cgctctctgt gtgtcagaaa gtgaccattt ctgtttgagc agcacaaaca
     4381 aaccagaata gcgtttccat tttttaccct ttttgagcga aggcgatcct ggtatacgct
     4441 catagtcttc gattggctct tcttctgcga ttggctcgcc ataaagcaaa tcacgcaatt
     4501 cttctccgac cagcaggcca atggtgaagg tcagcaacaa aagaccgacc attttctcca
     4561 tgtagacttg ttgcttattc atgagcttgg tcaaacccag caagcttttc aagtcgcggt
     4621 aagtctcttc aattttcata cgggtaaagt aaatctgtaa gcctcgcttg gcttccaggt
     4681 tggtcatcac ccacattggt tcgctcaacc ccttcttcca ggttccgaca acgttcatgc
     4741 acaccttgcc catgtaccag atgtggttga gaactacggt ttccccgggt gaaatcgtca
     4801 aggccacttc cttgccatca tcatcataga atttgggcgg atgggagcgc aagttgagac
     4861 ggatcaccca gttgatctgc tcttcgaaga ggttgagcat caattccagg tagctgaatt
     4921 ctcgatccaa caccaacggg cgtcccccca acatgtcctt caagccttca aatgctcgaa
     4981 aatgattgag gttacgcgaa tccaagccct gggcgatcgt tctcgatgaa tacgtaacca
     5041 tcccacacgg gatggcccgg cctcgatagg gtgtggctaa gatcaaggcc cagaaccctc
     5101 tggtcttccc gtctttcagc ttgcctacat attctgtttt ccaggcgtgc ggtcgctcaa
     5161 tctcggttgg gtcgccaatc acgaactctg catcttcttg aaacagccgc cacagggctg
     5221 tccgggggtc tgcttttccc aggaagcgct ggatcctctt gtaaccggct gcgctttcac
     5281 cctccatttt cacggcgatg tcggtcaagc gtaacgagcg cgcctcaaga atggccgccc
     5341 cgatttctcc tgcccgttcc gccagttcag cattgtcaaa caattccacc ataaattctt
     5401 gattgttgat aaacttgtcc atgaagcctc tgagttgata aagatggtct cggaaaacca
     5461 agtttatctt cttcggggct tcttgtttgt tactcatgtc cgaacttttg tccggacatc
     5521 agtcagatta gcgtgcgatg gatgatctgg atgtggaaag cggcagggtg acggtaaaga
     5581 cagcgccgcc ggatggatga ttggctgcgg tgacatcccc gcccatggca cgggccaact
     5641 ggcgggcaat tgccagaccc aggccagtgc cgccttcgtc acggctgcga gaacgctcgg
     5701 cgcggtagaa acgctcaaag atgtgcggca gagcctccgg cgcaatgccg gggccgctgt
     5761 cgtgaaccgt caacagtccg gtggacgcaa gcgcaatcgt aatccgcccg gcatcgggtg
     5821 tataacgcag ggcgttgctg agcaaattgc ccagaatttg ctcaatgcgg tcgggatcgc
     5881 ccagggcttg aagcggtgag gaaggctcga tggtcagctc aacattgcgg gctgatgctg
     5941 gcgcgtcgaa ccggttaacc acgcgccggg tgagggcaac caggtccacc gggacgcgtt
     6001 caacgggcag ttcacccgct tcggccaggg ccagggtgcg caggtccccc accagacggg
     6061 tcaatagatc tgtctgggcc agaatggcat tcaggttttc gggggtcatt ggattgacgc
     6121 catccaaaag cgcttcgagc tgggcgcgct gaactgccaa cggagtgcgc agctcgtgag
     6181 caatgtcggc ggtcatggag cggcggcgct cttcggactg ctgaagggaa gccgccatat
     6241 ggttgaaggt gcggcccagg tcagacagct catcacgccc acgaatggca acccgctggg
     6301 ataaatctcc agcagacatg gcatgggcgg cgcgggtgag ctggcgaatg gggcggatca
     6361 ggccggcgga caaggcgagc gccatcaaga gggcgatgcc taccccaacc agacccgccc
     6421 gcaaggcagc gtcgttgagg cgttcaatga ggggcagctc gtcgccctga cggtaaaagg
     6481 aaccggttga gggcagcaaa tagcccaccg ctgcaccctg gggtccggtt aatacaatgc
     6541 ccccggcgag atcatcagat gacagcactg agccttcggt ctgcccggag gcgtctatca
     6601 gcaccagacc ctgagaatcg gctaggcgca agcgctgact gccaccgcca tgtccgccgt
     6661 tgccgcccgg gccatggcct tctgccgggc ctgcgccaac ggacagcagg ctgtcggcgc
     6721 cctgccagga gccgtgttcg gcgtagtaag cttcaagatc ggagacggtt tcttccaggc
     6781 cttcaaatcc cccgcgcagc atataggttt ggatctgctg ccgggagtcc aggcggacaa
     6841 aaatgaccac gctggcaatg gcagcgaggg caaccagcac aagggagagg atgaatttgg
     6901 tgcgcatgtg caacctcaag gctccaaatc tggcgagggg ttatcgggtt tgcggaaacg
     6961 gtaacccacg ccaaagacgg tttcgatata gcgcgggatg cggggttcgg gttccagctt
     7021 agcgcgcagg tttttgatat gggcatcaat actgcgctca taaccttcat aagccacacc
     7081 ctgggcggcc tctaacaact gcaggcgaga gaacacccgg cccggctggg aggccagggc
     7141 ggcaagcaag ttgaattcgg aaggtgtaag gtcaatgggc tggtcgtctc ggcgaactga
     7201 atgcgcgtcc aggtcaatct cgatatttgc tatacgcatt atattgtttt gagtctcagt
     7261 caccgcccca cgctccgagc gtcgaagcac tgcccgcacc cgcgccacca cctcgcgcgg
     7321 ggagaaaggt ttgacgatat agtcatcggc gcccaactcc aggccgatca gacggtcggt
     7381 ctcttcggcg cgggccgtga ccatgatcag cggggtttcg gatttgcgcc ggatggcatg
     7441 agcgacatcc aggccatcca gaccgggcag gttcaggtcg agcagcacca ggtcgggatg
     7501 ttcacgctcc caggtttgca acccggtatc gccacgggcg gataatagga cctcaaatcc
     7561 ggctttttcg agataagcct ggaggatttt tgccagttcg ggttcatctt caacaattaa
     7621 gattttggtc attgcacagc ctcaacttct gggcttatta tggcgtcatt ttgaaaattc
     7681 attttgaacg atagcggaaa gggcttcgag caagtcggtg nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn tcattttgaa aattcatttt gaacgatagc ggaaagggct
     7861 tcgagcaagt cggtgcgcca atcgggcgag gacatggaga gccagtaggc atttttgccg
     7921 gcgcgagccg tgggaccaag gggtagaagc tggcgcggcg gggagccttc gggcaggagc
     7981 gggaagcgca gcaccatctg atcgccttca accgcgacca gattgagccc tgccagatag
     8041 gctcggagct tgacgcgcat ctggaaaaat aaattttcaa ccgattcggg cagggcacca
     8101 aaacggtcaa taaactcttc ccgcagagca tccacctcat cttcagattg aagatcggca
     8161 atgcggcggt aaagattgag gcgctgctgc tggtcgggga cataatcgag cgggatgccc
     8221 actgagagcg gcaaatcgac cgtgatggcc atgcgcaatt cgggagcgct caacatgcga
     8281 tcatcgggag cgggcaggcc gctgacagcg cgcatctgac ggactgcctg ggccagcagg
     8341 cgcgtgtaaa ggtgaaagcc aacgctggcg atgtagccgt gctggcgcat gcccaacaat
     8401 tcaccagcgc cgcgcatttc aaggtcgcgc atggcaatgg agtagccggc gccaagctgg
     8461 gtgttttcgg cgatgacttc caggcgttcc tggccttcgg gagtgggcgg cttgcggcgg
     8521 tggcggaaga aataggcata ggcgcgctga gcgccgcgcc caacccggcc gcggagctgg
     8581 taaagctggg ccaggccaaa cgcatcgccg cgatcaacaa tcaatgtgtt agcgttgggg
     8641 atgtccaggc cagattcgat gatggaggtg cagagcagca cgtcaatttc gccgcgagtg
     8701 aactcgtgca ttacgcgagc tagatccgac tcgtgcattt gaccatggcc aatgcccacc
     8761 cgggcctcgg ggacgagggc gttcaggtgg cgttccatgg catgaatgga ctgcacacgg
     8821 ttgtgaacaa aaaagacctg tccgccacgt tcaagctcac ggaggatggc ctgacgcacc
     8881 agacgcggcg agtaggggcc gatatgtgtg atgatgggca agcgctcttc cgggggcgtg
     8941 ttaatgttgg agatatcccg gacaccggtc agtgccatgt agagagtgcg gggaatcggc
     9001 gtggcggtga gggtcaggac atcaatttca gtgcgcagtt ttttgaagtg ctctttatgg
     9061 gtcacaccaa agcgctgctc ttcatcaaca atagcgaggc caaggtcctt gaactcgaca
     9121 tcgggctgca gcaggcggtg ggtgccgatg atgatatcca cctcacccaa cgccagcttg
     9181 agcaggattt cactctgctc gcgcggggtg cggaagcgcg agagcatttc gaccgtgacc
     9241 ggaaaagctg ccagccgctc acggaaagtt tcataatgct gctgggccag aac
//
LOCUS       DAOPVS010000074         9269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7492_length_9269_cov_58.431409, whole genome shotgun sequence.
ACCESSION   DAOPVS010000074 DAOPVS010000000
VERSION     DAOPVS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_9269_cov_58.431409"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKL11_07135"
     CDS             complement(<1..467)
                     /locus_tag="PKL11_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13415.1"
                     /translation="METGTLSQKKTLSSVLSKAQVAWWEALLLFGLGATAVLLHQALR
                     APLNLPGRHGLEWMALLILGRALSRSRVAGSITSVGAATFSVLPIWGAMHDPFIWLIY
                     LVPGLLMDFVFAKLPGMTGKLWFLALLGSLAHATKPLIRLVITLITGFPFGSL"
     gene            complement(415..1143)
                     /locus_tag="PKL11_07140"
     CDS             complement(415..1143)
                     /locus_tag="PKL11_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13416.1"
                     /translation="MRFLLGLDDTDNLESRGTGHRARMLGLTLAEAGLAQLIGITRHQ
                     LLVSPLIPYTSHNSSACLELEAPAANRGSIWQAARDFTQTDSAPGSDAGLCLADAAQA
                     AGLKTWGQRAKREVLQKTDALALAAEAGPWLAELTGDGGGIIGAAAGVGLRAGGSDGR
                     YLWLPGLRELTGRLPVREIMAQTGIGRICTPAGLDLPADALLDVGEWLRPVLLDAIPV
                     LLVEEDHDGNWHTLAKEDVKQRSQ"
     gene            complement(1144..1602)
                     /locus_tag="PKL11_07145"
     CDS             complement(1144..1602)
                     /locus_tag="PKL11_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13417.1"
                     /translation="MTLLKQLAGLMLALILLAGCTSTPAETPAATAAPAAGALPTLPA
                     GAVFQVIDANGVVTLFGGAELGELPLGQITVEEKVEEGPRLLDVLAAAGVTAFNEVTL
                     TGPASAMTLTADQVDDQVLLDLTNHGTVKLASPYVPKADWVKDITLIQVK"
     gene            1731..>2674
                     /locus_tag="PKL11_07150"
     CDS             1731..>2674
                     /locus_tag="PKL11_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HNZ13418.1"
                     /translation="MTQPNWGLSPVPFIPGQELNRRFYAEAVAPLLAQFFPGLPHTAG
                     LIGYGSDVLGFDTPMSRDHMWGPRLVLLLPEADFDAHRVAVDAALRQHLPLTFCGYPT
                     HFGPSDEAGVRLLQFIEQGPVDHLVALNTLPGFLQAELGLTPGEPLTETRWLTFPEQK
                     LLAVTAGAVYHDDLGLEGARQSLAYYPDEVWRYLLACQWTRISQEEAFVGRTGDLGDD
                     LGSRVITARLVQDLMRLAFLQARRYTPYAKWFGTAFAQLPLAEALLPDLQAALAADNW
                     PAREAALCAAARQLAEQHNALGLTPPLDTQPRSFHGRP"
     assembly_gap    2675..2774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2906..4180
                     /locus_tag="PKL11_07155"
     CDS             2906..4180
                     /locus_tag="PKL11_07155"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNZ13419.1"
                     /translation="MSSLDNVLAVILGGGRGARLYPLTKVRSKPAVPIAGKYRLIDIP
                     ISNCINSGIFHVAVLTQFNSVSLHRHITRTYQFDTFHLGWVQVWAAEQTIASEDWYQG
                     TADAVRKQLYEIRAARAKDVLILAGDHLYRMDYAAMSQFHWDNDADITVAVQPVPAGE
                     AGRFGILKVDATQHITRFAEKPRDPATLQEMVSRSDAGRPFLGSMGIYLFKIEALIEL
                     VEKTSFDDFGTHVIPNALQTHSVYAYHFDDYWEDIGTIRSFYDTNLALADPNPPFNFF
                     DQDRPIYTHARYLPGSTVENSSLENVLLAEGCCIQNADIRHSVIGVRSRICSNVRIVD
                     TVMMGADYYDNVACESIDGNVPIGIGNGAHVEGAIVDKNARIGSNVVIRPFPRGTDID
                     GENYVVRDGIVVIPKDATILPGAYIGPGMTSE"
     gene            4241..7189
                     /locus_tag="PKL11_07160"
     CDS             4241..7189
                     /locus_tag="PKL11_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNZ13420.1"
                     /translation="MTVSSHPSEKPAAEDFSVTWHALSAEEVLSSLGTQIEQGLTTEE
                     AEKRQAQYGLNQLVEAPRTTFLKMVIDQLNNFVIILLIVAAVVSALLGEWIDAGAILA
                     IVVLNAVLGVIQESRAEQALAALKKLAAPEAQVLRSGHRVSVPARELVPGDIVYLEAG
                     NFVPADIRLLEAVNLRAEEAALTGESVPVQKNATISLDRDIPLGDRKNTVFMGTVVNY
                     GRGRGVVTSTGMHTQLGMIATMLQSVEEENTPLQQRLDQLGKILGYGALAVCGLVFLV
                     GLIRALGSMGFAELFTTATGLEEVVDLFMIAVSLAIAAVPEGLPAVVTISLALGMREM
                     VHRHALIRRLSSVETLGSATVICSDKTGTLTQNEMTVTKLWVDGQFVDVTGTGYRPQG
                     EFMVDGKAVNLHQYPAAMTALWLGVLNNDAQLEVTPSEEGGDPVYRIIGDPTEGSIIV
                     AAAKAGLGLEQLIAAFPRENEIPFDSTRKRMVTMHLINDPRAEITPGTLYHHAQQYTI
                     AVKGAPDVVLNLCNRYQHISDDKALPLDEAARARILAANDTMTQGALRVLGVAYRAIP
                     ALPEEVDNDDLERDLIFVGLIGMIDPARTEVRPALETARGAGIRTIMITGDYPNTAKA
                     IAESIHLLSPGHQVLTGAQLNEMTDAELQHEVQHTDVFARVSPEHKMRIVDALRANDE
                     VVAMTGDGVNDAPAIKASDIGVAMGITGTDVAKETADMVLTDDNYASIVSAVEQGRII
                     YANIRKFVFFLLSSNVAEIMIIFLATLAGVPTPLTAIQLLWLNLLTDGAPALALAMEK
                     GDPDVMERAPRPKKEPIINKSMRLGIGIQTIMQTGAVLTAFAIGLIWELQHTAGITIP
                     DGMNPIWFLIQFNDWVHVADVKTAETMAFVTLSLCELFRAYTVRSERASVFRLGLFSN
                     KYMQYAVALSIVLMLLVVNVPFLQTVFNTHFLSWDKWLTVVGLALLPAIAEEFTKLYL
                     RKSEQRPARS"
     gene            complement(7364..9220)
                     /locus_tag="PKL11_07165"
     CDS             complement(7364..9220)
                     /locus_tag="PKL11_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ13421.1"
                     /translation="MVEIHHINEIRPRLLGEFEAMPFTPRTEAIAEAYRWGQQLHAGQ
                     QRLSGEPYFETHCVWVASLVDRLVANEAWTIAALLHDSVEDRDSSFDAIREHFPGGLG
                     EEVARVVDGVTKLSAPKDGRTREMATLAKIARFRDPGVFLVKLADKTHNMMTVGHMPP
                     EKRRQKAVEAIRAYGKLAGILNCYRWRRFLEDISFPHAEPETYTFVKSRIDGDPRLQP
                     VTINQTMNHLGELMDRAGIDGRVEIIVNGYWQAWQKLRRMARVRKTSLSNFGDLNDLI
                     SYRLIVNSNVETDCYLLLARVNRFLASFLDQDRFDDYIASPQNGYRALQVTGLMPGLG
                     AIEIAIATDEMEGENLWGIVYNIQQGRDISLYRPVEILTPSGGTRFVPEGSTVLDAVA
                     SIQQEFLLDKISAIKINDRLAHLWDRVQPGDLVEVVTGGQRLHPTEEWLGHCNASTQR
                     LVQAVLATEALKRQAEEGRQMLRPLLMARGIINLDDVQALEPDRLTNLLERLGCSGVE
                     DLYVAFGGGAIRPTELVDVLNEVGLTCETLQWTTINLEGTAASNRPGVLARLAGLVFN
                     AGGNILRSVNNTLPGGGFELRIVVRHLEAEKHAFLKQAFSECDVSLEWLEIV"
BASE COUNT         1722 a         2922 c         2771 g         1754 t
ORIGIN      
        1 aacagcgagc caaacggaaa acccgtgatc agtgtgatga ccagccggat gagcggcttg
       61 gtggcatggg ccaggctgcc cagcagcgcc agaaaccaca gcttgccggt catgcccggc
      121 agtttggcga agacgaaatc catcagcagg ccgggaacca ggtaaatgag ccagatgaag
      181 ggatcatgca tggcgcccca gatgggcagc acagagaagg tggcggcccc cacgctggtg
      241 atggaccctg ccacgcgcga gcgtgagagc gcccgcccca gaatcagcag cgccatccat
      301 tccagcccat gccgcccggg caggttgagc ggcgcccgca gcgcctggtg cagcaaaacg
      361 gcagtggccc ccagcccgaa cagcagcagc gcctcccacc aggcgacctg cgccttactg
      421 agaacgctgc ttaacgtctt cttttgcgag agtgtgccag tttccatcat gatcctcctc
      481 aaccaacaag acagggatag catctaaaag cacggggcgc agccattcgc ccacatccag
      541 aagcgcatcg gcgggcaggt ctaacccggc cggagtgcaa atacgcccaa tgccagtctg
      601 ggccataatc tcacggacag gcaggcggcc ggtcagctcg cgcaggcccg gcagccacag
      661 gtagcggccg tcactgccgc cagcgcgcag ccccaccccc gccgcggcgc caataattcc
      721 gccgccatcg ccggtcagtt cggccagcca ggggccagct tcagccgcca gcgccagggc
      781 atcggttttt tgcagcacct cccgcttggc gcgctgcccc caggttttca acccggcagc
      841 ctgggctgca tctgccaggc acagcccggc atctgatccg ggggcgctgt cagtttgcgt
      901 aaaatcacgc gccgcctgcc agatgctccc ccggttggcg gcgggcgcct ccagttccag
      961 gcaggctgag ctgttgtgcg aggtgtaagg gatcaacggg ctgacgagta actggtgccg
     1021 ggtgatgccg atgagctgcg ccaggccagc ttcggccagg gtcaatccca gcatccgggc
     1081 gcggtgcccg gtgccgcggc tttccaggtt atcggtatcg tccagtccga gtaaaaatcg
     1141 catctacttc acctgaatca gggtgatgtc tttcacccag tcggctttgg gcacgtaggg
     1201 cgaggccagt ttgaccgtac cgtgattggt caggtcgagc agcacctggt cgtccacctg
     1261 gtcggcggtc agcgtcatgg cgctggcggg gccggtcagt gtgacttcat taaatgcagt
     1321 gaccccagcg gctgccagaa catccagcag gcgggggcct tcttcaactt tctcttccac
     1381 ggtgatctgc cccagcggca gttcgcccag ttcggcgccg ccgaacagcg tgaccacacc
     1441 attggcgtca atcacctgaa agaccgcccc ggcgggcagg gttggcagcg ctccggctgc
     1501 gggggccgcg gtagcggccg gggtttctgc cggggtggag gtgcagccag ccagcaagat
     1561 caaggccagc atcagcccgg ccaattgttt caataatgtc attcattcct ccaaaaggcg
     1621 aagtgaatta atcatagccg gattgagggc taaagtcaat catattttgg cccgccaaat
     1681 gtgatacagt tagagcatcc ccggcaccag aggagcctga agacttttcg atgacccaac
     1741 ccaactgggg cctcagcccc gttcccttca tccccggaca ggaactgaac cggcggtttt
     1801 atgctgaagc cgttgcgccg ctgctggcgc agtttttccc gggcctgccg cacaccgctg
     1861 gcttgattgg ctatggctca gatgttttgg gctttgatac gcccatgtcg cgcgatcata
     1921 tgtgggggcc gcgcctggtg ctgctgctgc ccgaggccga ttttgacgcg caccgcgtcg
     1981 cggtggatgc cgccctgcgc cagcatttgc cactgacgtt ctgcggctat cccacgcatt
     2041 ttggcccatc cgatgaggca ggcgtgcgcc tgctgcaatt catcgagcag ggcccggtgg
     2101 atcacctggt ggcgctgaac acactgcccg gctttttgca ggctgagctg gggctgaccc
     2161 ccggcgagcc gctgactgaa acccgctggc tgaccttccc cgagcagaag ctgctggcgg
     2221 tcactgcggg ggcggtctat cacgatgatc tggggctgga aggggcacgc cagtcgctgg
     2281 cctactatcc cgatgaggtt tggcgctatc tgctggcctg ccagtggacg cgcatcagtc
     2341 aggaggaggc gtttgtgggc cgcaccggcg acctgggcga cgatctgggc tcacgggtga
     2401 ttaccgcccg gctggtgcaa gacctgatgc gcctggcttt tctgcaggcg cggcgctata
     2461 ccccctatgc caaatggttt ggcacggcgt ttgcgcagct gcccctggcc gaggccctgc
     2521 tgcccgacct gcaggctgcc ctggcggcgg acaattggcc cgcccgcgag gccgcgctgt
     2581 gcgccgcggc ccggcagttg gccgaacagc acaacgccct gggcctcacc ccgccgctgg
     2641 atacccaacc ccgctccttc cacgggcggc ctttnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnngcgggc tgtttaaaat cacgacataa gtcacgccat ttaagtttga
     2821 atcggacgca cccattaaaa tgggctttgt gctatactct cggtgtacaa gtgactttta
     2881 atttaatgag ccggaggagg agtctatgtc atcgctggat aatgtgctgg ctgtgattct
     2941 gggcggtggg cgcggcgccc gcctgtaccc tttgaccaaa gtgcgctcga agcctgccgt
     3001 gccaattgcg ggcaagtacc ggctcattga tattcccatc agcaactgca tcaactcggg
     3061 catcttccac gttgccgtgc tgacgcagtt caactcggtg tcgctgcacc ggcatatcac
     3121 ccgcacctac cagtttgata ccttccacct cggctgggtg caggtctggg cggcggagca
     3181 gaccatcgcc agcgaagact ggtaccaggg caccgccgat gccgtgcgca agcagctcta
     3241 cgaaattcgc gccgcccgcg ccaaagatgt gctcatcctg gcgggcgatc acctctaccg
     3301 catggattac gccgccatgt cccagttcca ttgggataat gacgccgaca tcaccgtggc
     3361 tgtgcagccc gtgcccgccg gcgaagccgg gcgctttggc atcctcaagg tggatgccac
     3421 ccagcacatc acccgctttg ccgaaaaacc ccgcgacccc gccaccctgc aggagatggt
     3481 cagccgcagc gatgccgggc gccccttcct cggctcgatg ggtatttatc tgttcaagat
     3541 tgaagccctg attgagctgg tggaaaagac ctcttttgac gattttggca cgcatgtcat
     3601 tcccaatgcg cttcagaccc actcggtgta tgcctatcac tttgacgact actgggaaga
     3661 catcggaacg atccgctcgt tttatgatac caacctggcg ctggcagatc ccaacccgcc
     3721 tttcaacttc tttgaccagg atcgccccat ctatacccac gcccgctacc tgcccggctc
     3781 aaccgtagaa aacagctcgc tggaaaatgt gctgctggct gaaggctgct gcatccaaaa
     3841 cgccgatatc cgccactcgg tgatcggtgt gcgcagccgc atttgcagca atgtgcggat
     3901 tgtggataca gtgatgatgg gcgctgacta ctatgacaat gtcgcctgcg agtcaatcga
     3961 tggcaatgtg ccgattggca ttggcaacgg cgcccatgtg gaaggcgcca ttgtggataa
     4021 aaacgcccgc atcggcagca atgtggtcat ccggcccttc ccgcgcggca ccgatattga
     4081 cggcgaaaat tatgtggtgc gggatggcat tgtggtcatc cccaaagatg cgaccattct
     4141 ccccggcgcc tatatcggcc cggggatgac aagcgaataa ccccaggcct atccccgggc
     4201 ggctcagccg cccttaatcc ttattgttgg agcatagcct atgactgtat cgtctcatcc
     4261 ctcggagaag cccgccgcag aggacttctc ggtaacctgg cacgcgctga gcgccgaaga
     4321 agtgctgagc agcctgggta cacagatcga acagggcctg accaccgaag aagcggaaaa
     4381 gcgccaggcg cagtacggtc tgaatcaact ggtggaagcc ccccgcacca ccttccttaa
     4441 aatggtcatt gaccagctca ataattttgt cattattctg ctgatcgtgg cggcggtggt
     4501 ctcggccctg ctgggcgaat ggattgacgc cggcgccatc cttgccattg tcgtcctcaa
     4561 tgcggttctg ggcgtcattc aagaaagccg ggctgaacag gccctggcgg ccctgaagaa
     4621 gctggcggcc cctgaagcgc aggtgctgcg cagcgggcac cgcgtttccg tgccggcgcg
     4681 cgagctggtg cccggcgata tcgtctatct ggaagcgggt aattttgtgc ccgccgatat
     4741 tcgcctgctg gaagccgtga acctgcgcgc cgaagaagcc gccctgacgg gcgaatcggt
     4801 gccggtgcag aaaaacgcca ccatctcgct tgaccgggat attcccctcg gtgaccgcaa
     4861 gaacacggtc tttatgggca cggtggtcaa ctacggccgc ggccgcggtg tggtgaccag
     4921 caccggcatg cacacccagc tcggcatgat cgccaccatg ctgcaaagcg tggaagaaga
     4981 aaataccccc ctgcagcagc gcctggatca actgggcaag attttgggct acggcgcgct
     5041 ggcggtctgc ggcctggtct tcctggttgg gctgatccgc gccctgggtt ccatgggctt
     5101 cgctgaattg ttcaccaccg ccaccggcct ggaagaagtg gtggatctgt tcatgatcgc
     5161 tgtcagtctg gcaattgctg ccgtgcccga gggcctgccg gcggttgtga ccatcagcct
     5221 ggcgctgggc atgcgcgaga tggtgcaccg ccacgcgctc atccgccgcc tctcctccgt
     5281 agagacgctg ggttcagcca ccgtcatctg ctcggataag accggcaccc tcacccagaa
     5341 cgagatgacc gtcaccaagc tctgggtgga tggtcagttt gtggatgtga ctggcacggg
     5401 ctaccgcccg cagggcgaat ttatggtgga tggcaaagct gtgaacctgc accagtaccc
     5461 cgccgccatg accgccctct ggctgggcgt attgaataac gatgcccagt tggaagtgac
     5521 gccctccgaa gaaggcggcg accccgtgta ccgcatcatc ggcgacccca ccgaaggctc
     5581 cattattgtg gccgcggcca aagccgggct gggcctggag cagttgattg ccgccttccc
     5641 gcgcgagaac gaaatcccct ttgactcgac ccgcaaacgc atggtcacca tgcacctgat
     5701 caacgacccg cgcgccgaga tcaccccggg cacgctgtac catcacgcgc agcagtacac
     5761 cattgccgtc aagggtgcac cggacgtggt tttgaacctg tgcaaccgct atcaacacat
     5821 ttctgacgac aaggccctgc cgctggatga ggccgcccgc gcccgtattc tggccgccaa
     5881 cgacaccatg acgcagggcg cgctgcgggt gctgggggtg gcctaccgcg ccatcccggc
     5941 cctgcccgaa gaggtggata acgatgatct ggagcgggac ctgatctttg tgggtctgat
     6001 cggcatgatt gacccggccc gcaccgaagt ccgcccggcg ctcgaaaccg cccgcggcgc
     6061 cggaatccgc accattatga tcaccggcga ctaccccaac accgccaagg ccattgccga
     6121 gagcattcat ctgctctcgc cggggcacca ggtgctgacc ggtgcgcagt tgaatgagat
     6181 gaccgatgcc gagctgcagc acgaagtgca gcataccgat gtctttgccc gggtttcgcc
     6241 ggaacataaa atgcgcattg tggatgcgct gcgcgccaac gatgaggtgg ttgccatgac
     6301 cggcgacggc gtcaacgatg cgccggcgat caaggcctcg gatattggcg tggcgatggg
     6361 catcaccggc accgatgtgg cgaaagaaac cgccgacatg gtgctgaccg atgataacta
     6421 cgccagcatc gtctcggcag tggagcaggg gcgcatcatc tatgcgaaca tccgcaagtt
     6481 tgtgttcttc ctgctgtctt ccaacgtggc cgagattatg atcatcttcc tcgccacgct
     6541 ggcaggcgtc cccaccccgc tgacggccat ccaactgctg tggctcaacc tgctgaccga
     6601 tggcgccccg gccctggccc tggcgatgga aaaaggcgac cccgatgtga tggagcgcgc
     6661 cccgcggccc aagaaagagc cgatcatcaa caaatccatg cggctgggca tcggcattca
     6721 aaccatcatg cagaccggcg ccgtgctgac cgcctttgcc attggcctga tctgggagct
     6781 gcagcatacc gccggcatca ccatccccga tggcatgaac ccgatctggt tcctcatcca
     6841 gttcaacgac tgggtgcatg tggccgatgt gaagaccgcc gagacgatgg cctttgtcac
     6901 gctctcgctg tgcgagctgt tccgtgccta caccgtgcgt tctgagcggg cctcggtctt
     6961 ccgcctgggc ctgttctcca acaagtacat gcagtacgct gtggcgctgt ccattgtgct
     7021 gatgctgctg gtggtcaatg tgcccttcct gcagaccgtc ttcaataccc acttcctgtc
     7081 ctgggataag tggctgacgg tggttggcct ggcgctgctg ccagccattg ccgaagagtt
     7141 caccaagctt tacctgcgca agagcgagca gcggcccgcc cgcagctaac cgggctgaac
     7201 ccgctaaaat gaccccgccg gagccaaacc ctccggcggg tttgtttaac ctgccgggct
     7261 gtggcgcctt tcgttggggg ttaagtatcc gattttgggt gtcgcccggt gacttttatg
     7321 atgacacgcg gccgcatgtg gaacctggta cgcagaacgc cccctacaca atctccagcc
     7381 attcgaggga tacatcgcac tcgctgaagg cctgcttgag gaaggcgtgc ttttcagcct
     7441 ccaggtggcg caccacaatc cgcagctcaa acccgccgcc gggcagggtg ttgttcaccg
     7501 agcgcagaat attgcccccg gcattgaaca ccagccccgc cagccgggcc agcacccccg
     7561 gacggttgga tgcagcggta ccttccaggt tgatcgtcgt ccactgcagg gtttcgcagg
     7621 tcaggcccac ctcgttcagc acatccacca gctcggtcgg gcggatggcc ccgccgccaa
     7681 aagccacata caggtcttca accccgctgc agcccagccg ctccagcaga ttggtcaggc
     7741 ggtcgggttc cagcgcctgc acatcatcca ggttgatgat gccgcgcgcc atcagcagcg
     7801 ggcggagcat ctggcggccc tcctccgcct ggcgtttgag cgcctcggtg gccagcacgg
     7861 cctgcaccag ccgctgggta gaggcgttgc agtgtcccag ccattcttcg gtggggtgca
     7921 ggcgctgccc gcccgtcacc acttccacca ggtcgccggg ctgcacccgg tcccacaggt
     7981 gggccagccg gtcgttaatt ttgatggcgc tgatcttgtc gagcaaaaat tcctgttgaa
     8041 tggaggccac cgcatccagc acggtggagc cttcgggcac aaagcgggtg ccgccagagg
     8101 gggtcaaaat ttccaccggg cggtacaggc tgatatcgcg cccctgctga atgttgtaca
     8161 caatgcccca caggttctcg ccttccattt cgtcggtggc aatggcaatt tcaatcgcgc
     8221 ccaggccggg catcaacccg gtgacctgca gggcacggta gccgttctgc gggctggcaa
     8281 tataatcatc aaaacgatcc tgatccaaaa atgaggccaa aaaacggttg acccgcgcca
     8341 gcagcaggta gcagtcggtt tccacattgc tgttcacaat caggcggtat gaaatcaggt
     8401 cgttcaggtc gccaaaattg ctcaacgagg tcttgcggac gcgcgccatg cggcgcagct
     8461 tctgccaggc ctgccagtaa ccgttgacaa tgatctccac gcggccgtca atgccggcgc
     8521 gatccatcag ctcgcccagg tggttcatgg tctgattgat ggtcacgggc tgcaggcggg
     8581 ggtcgccgtc aatgcggctt ttgacaaagg tataggtctc gggctcggca tgggggaagg
     8641 agatatcttc caggaagcgg cgccagcggt agcagttgag aatgcctgcc agcttgccat
     8701 aggcccgaat ggcctccacg gccttctggc ggcgcttttc gggcggcata tggcccaccg
     8761 tcatcatgtt gtgggtcttg tcggccagct tgaccaggaa aacgccgggg tcgcggaagc
     8821 gggcgatctt ggccagggtg gccatctcgc gggtgcggcc atctttaggc gcgctgagct
     8881 tggtcacgcc atccaccacg cgggccacct cctcgcccag cccgccgggg aagtgctcgc
     8941 gaatggcatc aaagctggag tcgcggtctt ccacgctgtc gtgcagcagg gcggcaatcg
     9001 tccaggcttc gttggcgacc aaccggtcca ccagcgaggc cacccagacg cagtgggttt
     9061 caaagtaggg ttcgccgctc aggcgctgct gccccgcgtg aagctgctgc ccccagcggt
     9121 aagcctcggc aattgcctcg gtgcgggggg tgaagggcat ggcctcaaat tcccccagca
     9181 accgggggcg gatttcgttg atgtgatgga tttcaaccat gggcggctcc tgtacgctta
     9241 attatagcac tgctgaaaag acgcggcgt
//
LOCUS       DAOPVS010000075         9245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7514_length_9245_cov_59.530250, whole genome shotgun sequence.
ACCESSION   DAOPVS010000075 DAOPVS010000000
VERSION     DAOPVS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_9245_cov_59.530250"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKL11_07170"
     CDS             complement(<1..395)
                     /locus_tag="PKL11_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13422.1"
                     /translation="MRETPAAYRVSDMEANDRPRERLAEVGAENLSTAELLAILLRTG
                     LPGENVVALAGRLLKDMGGLSGLHRSSLQELRDQHGLGLAKATQIKAAIELGRRLASE
                     KPEDRPAITSPRDVADIVKYEMSAFTQEHL"
     gene            508..1035
                     /locus_tag="PKL11_07175"
     CDS             508..1035
                     /locus_tag="PKL11_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ13423.1"
                     /translation="MNFDPSFISSFTLIAVAFAAAFTVALWLSLIFWTYRDIRQRSRD
                     PLAKILAVLVVAVLFLPGILVYLILRPPHTLEDEYQQTLEEEALLQSIEDNPLCPGCG
                     RRIQADWVACPNCHTRLRKRCHQCNKLMELPWNLCPYCGTPAPGMRRENPTLDDALQP
                     LNIEESEPTEQPTGE"
     gene            1039..1896
                     /locus_tag="PKL11_07180"
     CDS             1039..1896
                     /locus_tag="PKL11_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNZ13424.1"
                     /translation="MQSLMRPEPIDYLVIGHITEDLTPAGPTPGGTVSYAALTAKALG
                     LRVGIVSSHAAGAPLQPLEGIPIAYLPAEHSTTFENQTTPEGRVQYLYHAAPMLDLSL
                     VPEPWRSAPIVHLGPVAQEVDPKIVWGFPDSFIGVTPQGWLRSWNDEGRVHPVEWPEA
                     AFVLAKASAAVLSIEDVQHNEDWIEDMLGSIRILVVTEGAAGARLYWNGDVRYFRAPV
                     MKEVDATGAGDIFATAFFARLFKTRDPWEATRFATQLAAISVTRPGLLGIPTPAEVQT
                     SLMEILPKV"
     gene            1903..2697
                     /locus_tag="PKL11_07185"
     CDS             1903..2697
                     /locus_tag="PKL11_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNZ13425.1"
                     /translation="MPRIYTCANQKGGVGKTTCTINLGAYLGYYGQRVLIVDLDPQAN
                     ATSSLGIDKRTVRQGTYEVLIGSVTLGPHMILHNPRLKISLLPASSALAGAEVELIDL
                     PQREQRLKQALTPILDRYDYILIDCPPSLSLLTINGLVAGKHGVIIPVQCEYLALEGL
                     GQLAGTIQRVQAGLMPELTIRGVIMTMYDGRTHLSNDVVQEVRRVFPNQVFQSVIPRS
                     VRLAEAPSHGLPISAYAPDSSAAQSFAALAREILEQDGIHIPDLKE"
     gene            2704..>3239
                     /locus_tag="PKL11_07190"
     CDS             2704..>3239
                     /locus_tag="PKL11_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ13426.1"
                     /translation="MARKSGLGKGLDALIPGGDDNPAPEGSAVFIPIAQIIPNPRQPR
                     GEMNSEALAELAASIREHGILQPLMVTYDPDTNQYILIAGERRLRAAHMVGLQSVPVL
                     VRQITERERLELALIENIQRADLTPLDLAEAYRQLQDEFGLSHEEISVRVGKSREAVT
                     NTLRLLKLPEAVRQALSVG"
     assembly_gap    3240..3339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3340..3716
                     /locus_tag="PKL11_07195"
     CDS             <3340..3716
                     /locus_tag="PKL11_07195"
                     /inference="COORDINATES: protein motif:HMM:NF036800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage 0 sporulation protein J"
                     /protein_id="HNZ13427.1"
                     /translation="TLRLLKLPEAVRQALSVGQISEGHARALLGLNTPQSQISALQTI
                     IHLDLNVRQTENLVRQLKGERLTLPSRKANPPEITALEERLQHALGERVILRHGKKGG
                     SVVIRYYSDEELNALVERMTRG"
     gene            3719..5302
                     /locus_tag="PKL11_07200"
     CDS             3719..5302
                     /locus_tag="PKL11_07200"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNZ13428.1"
                     /translation="MQILYNAKIRTQNSKQPTATALAIEGGRIVAVGSDYELLAMSDR
                     KAKKEDLGGRTIWPGLTDGHFHLEYYALGLQKVDVETETYAECLRRVNERDQNTPAGR
                     WVLGHGWNQNLWPEGFGTAKDLDRVAPNHPVYLTAKSLHAGWANTAALRLAGITANTP
                     NPEGGEIQRNAQGEPTGILLENAMDLLSRAIPTPTITDVIEAVRQAQPRLWQMGLTGG
                     HDFDGPRCFAALAHLEAENALGLRILKGIRQSSLDSASELRLRGGYGSEMLWFGAVKL
                     FADGALGPQTAAMLAPYEGTDHSLGMALLTAEEIFEIGKQAAQSRLGLAIHAIGDNAN
                     RIVLDGYAKLRQWEKETGHPHLRHRIEHVQVLHPEDLGRLAELEIIASMQPIHATSDM
                     EIADRYWGNRAALAYAFQSLLQSGARLSFGSDAPVESPNPFWGIHAAVTRRRMNGKPG
                     PDGWRGEQRLNLQDALDAYTTGPAFAAGRENWLGQISPGFAADLIVLPLDPATMPPEN
                     LYTVQPDATMVNGNWVWSR"
     gene            5302..>5506
                     /locus_tag="PKL11_07205"
     CDS             5302..>5506
                     /locus_tag="PKL11_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13429.1"
                     /translation="MTIELFPHIPPFPESSVPLKPEVLVPRLGDALIEKGLITQVDLD
                     RALEYQSKQRYDGQTPLLGQVLVE"
     assembly_gap    5507..5606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5607..6515
                     /locus_tag="PKL11_07210"
     CDS             <5607..6515
                     /locus_tag="PKL11_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ13430.1"
                     /translation="SKQRYDGQTPLLGQVLVELGMIDQSDLDQAVTEQIMLLRAALED
                     SNRQLERRVEQRTAELKNALDKLAEMSQLKSNFIANISHELRTPLTHLTGYLDLLVSG
                     ELGGVTPDQNHALVIIQRAGYRLHRLIEDLILFSLSERGEVKILLEPVNLSTVCQTVV
                     ERNRALAAERNISLLLHCPTIMPPAMADQEMITWVIAQLVDNAIKFSNPNGRVKLEVM
                     VEVAEIRVSVSDEGIGIPADRIEEIFQPFHQLDGSSTRRYGGTGLGLALARKIIESHG
                     SNIEVESHLGEGSRFSFLLTAAPEGV"
     gene            6523..7755
                     /locus_tag="PKL11_07215"
     CDS             6523..7755
                     /locus_tag="PKL11_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ13431.1"
                     /translation="MSKDTAELFPTIQLDDIYAICQLAKQARSWRPALVQILKQVRKT
                     IIYDNVAVYLLDRNTGSLEVSYAKSVGRGRSSEADVAWGEVAAGLVFQTGDKVLQEPE
                     PNPNLDRLEQPFILGMPLKLEQEILGAMVFIRFGGPHYTPEQIEYASFTAQLAGCMIG
                     VQELKQQLSTVETLKQMLDIQGDLISILSHEIRNPLGFIKGYTSTLLRSDTEWDAQTQ
                     REFLTIIESETDRLERLLENLLDSARLESGQTIIKFLPVRLEALFNDMKERAKLQYPD
                     MQIEVQIETSPATVLGDPRRLTQVFENLLSNSAKYAPGSPVHINVQQMDQPKGILIRF
                     SDSGPGIPEKHLPFIFERFFRNPEQNLQIYGTGLGLYIARQIVLAHHGRITATSDAGK
                     GVTFHIFLPEQTFEPPVA"
     gene            7758..8459
                     /locus_tag="PKL11_07220"
     CDS             7758..8459
                     /locus_tag="PKL11_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13432.1"
                     /translation="MSALILVVDDEALYQRLIRVNLEAEGYQVNTAGDGEAGLEAIAQ
                     KMPDLVILDVMMPKLDGFTTCERIRQFSSVPIIMLTAKAEEEDRVRGLNAGADDYIAK
                     PFSATELVARVRAVLRRAQRADQFTQNRYFTHGNLRIDFARAEIWKDDKAIYLSATEY
                     RLLIQFAQHVGQVLTAEDLLLSVWGEQYREDKEILWVSIARLRQKLEDNPHSPQHIVT
                     RAGLGYLMPPSDSNA"
     gene            8480..8776
                     /locus_tag="PKL11_07225"
     CDS             8480..8776
                     /locus_tag="PKL11_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13433.1"
                     /translation="MVTSFDEKGKIFTQVVRKQPTQVTIQTLDYQIRGQVHVHPDSRL
                     KDEIEREEHFVALTDVTLHDKSGALVMRTAFIAVNKAQIIWIVPDEDIAKEKQE"
     gene            8773..>9245
                     /locus_tag="PKL11_07230"
     CDS             8773..>9245
                     /locus_tag="PKL11_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNZ13434.1"
                     /translation="MSPLQTTQTPQAAKPPELLRLKEHLISLISRELEMRSPPMNERR
                     EAIQQIMASAYQGLRLSLPNTLRDQLFHEIMDDLMGFGPLQPLLDDPDITEVMVNGPK
                     KVYVERKGKITKTNVTFDDDNAVIRVIEKIILPLGRRIDGDSPTVDARLPDGSRV"
BASE COUNT         2043 a         2373 c         2764 g         1865 t
ORIGIN      
        1 agatgttcct gggtgaaggc actcatctcg tatttgacaa tatcagccac atcccgcggg
       61 ctggtaatgg cggggcggtc ttcgggtttt tcagaggcca ggcggcgccc cagctcgatg
      121 gcggctttga tctgggtggc tttggcgagg cccaggccgt gctggtcgcg cagttcttgc
      181 agtgaagagc ggtggaggcc gctcaaaccg cccatatctt tcaagagccg cccggcaagg
      241 gcgaccacgt tttcgccggg caagccagtg cgcagcaaaa tggcaagcag ctctgccgtg
      301 ctgaggtttt cggcgcccac ttcggcaagc cgctcacgcg ggcgatcatt ggcttccatg
      361 tctgacactc gataagcggc gggggtttct cgcatggcat cacctttccc aagtctggag
      421 atactatatc gcaggaggga taaaactgca agccaccgca cgtcagaaac gctcatccat
      481 gctataattt caccattgga gcatgttatg aattttgacc cctcatttat cagcagtttc
      541 acattaattg ccgtagcctt tgcggcggcg ttcaccgtgg cgctctggct cagcctgatt
      601 ttctggacgt atcgggatat tcggcagcga tcgcgcgatc cgctggcgaa aattctggcg
      661 gtgctggtgg tggcagtgct gtttctgcca gggattctgg tgtacctgat tttgaggccg
      721 ccacacacgc tggaagatga gtatcaacag acgctggaag aagaagccct gctccaatcg
      781 attgaggata atccgctgtg cccgggctgc gggcggcgca ttcaggcaga ctgggtggcc
      841 tgtcccaact gtcacacccg actgcgcaag cgctgccatc agtgcaataa attgatggag
      901 ctgccctgga atttgtgccc ctactgcggc acccctgccc cgggaatgcg gcgcgagaac
      961 cccacgctgg acgatgccct gcaaccgctc aacatagaag aaagcgaacc caccgaacag
     1021 ccaactggcg aataacccat gcaaagtttg atgcgccccg aacccatcga ttacctggtc
     1081 atcggtcaca ttactgagga cctgaccccc gccggaccta cgcccggcgg tacggtctct
     1141 tatgcggcgc tgacggcaaa ggcgctgggg ctgcgggtgg ggattgtttc atcccatgcg
     1201 gcgggggcgc cgctacagcc gctggaaggg ataccgattg cttacctgcc agcggaacat
     1261 tcgacgacat tcgaaaatca gaccacgcca gagggacggg tgcagtatct ctatcatgcg
     1321 gcgcccatgc tggacctctc gctggtaccc gaaccctggc gcagcgcgcc cattgtgcac
     1381 ctggggcccg tagcccagga agttgacccc aagattgttt gggggtttcc ggattcgttt
     1441 atcggggtga cgccgcaagg ctggctgcgc tcgtggaacg atgaagggcg agtgcacccg
     1501 gtggaatggc cggaagccgc ttttgtgctg gcgaaagcca gcgcggcggt gctcagcatt
     1561 gaagacgtgc agcacaacga agattggatt gaggatatgc tgggatccat ccggattctg
     1621 gtcgtgaccg aaggcgcggc gggcgcccgc ctgtactgga acggcgatgt acgttacttc
     1681 cgggcacctg tgatgaagga agtggacgcc accggcgccg gcgatatttt tgccaccgcc
     1741 ttttttgccc gattattcaa aacgcgcgat ccgtgggagg caacccgctt tgccacccag
     1801 ctcgcagcaa tatccgtcac ccgccccggc ttgctgggta tccccacacc ggctgaagtg
     1861 caaacatctc tgatggaaat tctccccaag gtataaacaa acatgccccg gatctacacc
     1921 tgtgccaatc aaaaaggggg ggtcggcaag accacctgta ccatcaattt gggcgcctac
     1981 ctgggctatt acggtcaacg ggttctcatt gtggacctgg acccgcaggc caacgccacc
     2041 tcatcgctgg ggattgacaa acggactgtg cgccagggta cttatgaggt gttgattggg
     2101 tcggtaacgc tgggcccgca catgattttg cacaatccgc ggttgaaaat ttcactgctg
     2161 ccagcctcat cggcgctggc gggggccgaa gttgaattga ttgacctgcc ccagcgggag
     2221 cagcgtttga agcaggcact gacgccaatt ttggaccggt acgattatat tttgattgac
     2281 tgcccgccgt cgttaagcct tttaaccatc aacggactgg tggcaggaaa gcacggcgtg
     2341 atcattccgg tgcagtgcga atatctggcg ctggaagggc tggggcaact ggcgggaaca
     2401 attcaacgcg tgcaagccgg gctgatgccc gagttaacaa tccgaggggt gattatgaca
     2461 atgtatgatg gccggacgca cctgtcgaat gatgtggtgc aggaagtcag acgggttttc
     2521 ccaaatcagg tttttcaaag cgtgattcca cggagtgtgc ggctggccga ggcgccctcg
     2581 cacgggctgc cgatctcagc ctatgcgcct gattcgagcg cggcgcagtc ttttgcggcg
     2641 ctggcccggg aaattttgga acaagatgga attcatatcc cggatttgaa ggagtaggat
     2701 cacatggcgc gaaaatctgg tctgggaaaa ggtttggatg cattaatccc cgggggcgat
     2761 gacaacccgg cgccggaagg cagcgcggtt tttatcccaa ttgcgcagat tatccccaat
     2821 ccacggcagc cacgcggtga gatgaacagt gaagcgttgg ctgagttagc ggcatcgatt
     2881 cgagaacatg gaattttgca gccgctgatg gtgacctatg atccggacac caatcagtat
     2941 attttgattg ctggcgaacg ccggctgcgc gcagcccaca tggtggggct gcaatcggtg
     3001 ccagtactgg tgcggcagat cacggaacgg gagcggctgg aactggctct gattgagaat
     3061 attcagcggg cagatttgac gccgctggac ctggctgagg cctaccgcca gcttcaggat
     3121 gaatttggcc tttcgcacga agaaatttcg gtgcgggtgg gcaagagccg tgaggccgtg
     3181 accaacaccc tgcgactgct caaactgccc gaggccgtgc ggcaggcgct atcggtgggn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna caccctgcga ctgctcaaac
     3361 tgcccgaggc cgtgcggcag gcgctatcgg tggggcagat cagcgagggg cacgcccggg
     3421 cactgctggg attgaacacg ccgcagtcac aaatctcggc cctgcagacg attatccacc
     3481 tggacttgaa tgtgcggcag accgagaatc tggtccggca gttgaagggc gagcggctga
     3541 cgctgccttc ccgcaaggcg aatccgccag agattacggc gctggaagaa cgattgcagc
     3601 atgccctggg tgagcgcgtg atcttgcgac acgggaaaaa aggcggcagc gtggtgatcc
     3661 gctattattc tgacgaggaa cttaacgcac tggttgaacg aatgacgcga ggctgacaat
     3721 gcagatactc tataacgcca aaattcgaac tcaaaattcc aagcagccca ctgcaacggc
     3781 gctggcaatt gaagggggaa gaattgtcgc ggtgggcagt gattatgaac tgctggcaat
     3841 gagcgaccga aaggcgaaaa aagaggatct gggcggcagg actatctggc cgggtttgac
     3901 cgatggccat tttcacctgg agtattatgc tctggggctg caaaaggttg atgttgagac
     3961 agagacctat gccgagtgcc tgcgccgcgt gaacgagcgc gatcaaaata cacccgccgg
     4021 gcgctgggtg ctggggcatg ggtggaatca aaacctgtgg ccggaaggct ttggcacggc
     4081 caaagacctg gaccgtgtgg cgccaaatca cccggtgtac ctgaccgcaa aatcactcca
     4141 tgcaggctgg gcaaataccg ccgcactccg gctggcgggc attacagcca atacccccaa
     4201 cccggaaggg ggagaaattc aacgcaatgc ccagggagag ccgacaggca ttctactgga
     4261 aaacgccatg gatctcttga gccgggccat tccaacgccg acgattacag atgtgattga
     4321 agctgtccgg caggcgcagc ccaggctctg gcagatgggg ctgaccgggg gacacgattt
     4381 tgacggcccg cgctgctttg cggcgctggc gcacctggaa gcagaaaatg cactgggctt
     4441 gcggattctc aagggcatcc ggcaaagcag cctggactcc gccagcgaac tgcggctgcg
     4501 gggtggttac ggcagcgaga tgctctggtt tggggcggtg aaattatttg cagacggggc
     4561 gctggggcca caaacggccg cgatgctggc cccctacgag ggcaccgacc acagcctggg
     4621 tatggcactg ctaacagcag aggaaatctt cgagattggc aagcaggcag cccagagccg
     4681 gctggggtta gccatccatg cgattggcga caacgccaac cggattgtgc ttgatggata
     4741 tgcgaaatta agacaatggg aaaaagaaac cgggcacccg catttgcggc accggattga
     4801 gcatgtgcag gtactgcacc ctgaagacct ggggcggctg gctgaactgg agattatcgc
     4861 ctccatgcag ccgatccacg cgacatctga catggagatt gctgaccggt actggggaaa
     4921 ccgggcggcg ctggcgtatg ccttccaaag cctgcttcaa agcggggcgc ggttgtcgtt
     4981 tgggtcggat gcgccagtgg agtcacccaa cccgttctgg ggaatccatg ctgcggtaac
     5041 ccggcggcgg atgaatggca agccggggcc tgacggctgg cgcggcgaac aacggcttaa
     5101 tttgcaagat gcattagatg cttacacaac gggcccggcc tttgctgctg gacgcgaaaa
     5161 ttggctcgga caaatctcac cgggctttgc tgccgacctc atcgttctgc cacttgatcc
     5221 agcgaccatg ccgccggaaa acctgtacac cgtgcagccg gatgccacca tggtcaatgg
     5281 aaactgggta tggagccgct gatgaccatt gaactatttc cccatattcc accttttccc
     5341 gaatcgtcag tgcctttgaa gccagaggtg ctggtgcccc gcctgggcga tgccctgatt
     5401 gaaaagggat tgatcacaca ggttgacctt gaccgggcgc tggaatatca gtccaaacag
     5461 cgatatgatg gacaaacgcc gctgttaggg caggtgctgg ttgaacnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnntcca aacagcgata tgatggacaa acgccgctgt
     5641 tagggcaggt gctggttgaa ctgggcatga ttgatcagag tgacctggac caggctgtaa
     5701 ccgaacaaat tatgctgctg cgggcggcac tggaagacag caaccgccaa ctggagcggc
     5761 gggttgaaca gcgaacggct gagttaaaaa atgcgctgga taagctggca gaaatgagcc
     5821 agttgaaaag caattttatt gccaatattt cgcatgagct gcgaacaccg ctaacccacc
     5881 tgactggcta tttggatttg ctggtctcgg gcgaactggg gggcgttacg ccggatcaaa
     5941 atcatgcgct ggtcatcatt caacgcgcgg ggtaccggct gcaccgcttg attgaggatc
     6001 tgattttatt ctcgctcagc gagcgcggcg aggtgaagat tttgctggag ccggtcaacc
     6061 tttcaaccgt ttgccagacc gtggtagagc gcaaccgggc actggcggca gaacgcaata
     6121 ttagcctgct gctgcactgc ccgactatta tgccgccggc gatggctgac caggaaatga
     6181 tcacctgggt gattgcccaa ctggtggata atgccatcaa attctccaac cctaacggac
     6241 gggtaaagct cgaggtgatg gttgaggtgg cggaaatccg ggtctcggtc agcgacgagg
     6301 gcattggcat tccggcagac cggattgagg aaattttcca gccgttccat cagttggacg
     6361 gctcgtctac ccgccgatac ggcgggactg gattgggcct ggcgctggcg cgcaagatca
     6421 tcgaatcgca cgggagcaat attgaggttg aatctcattt aggcgagggc agccggttct
     6481 ctttcctgct taccgcagcc ccggaaggtg tataatttta aaatgagcaa agatacagcg
     6541 gaattatttc ctacgatcca gcttgatgac atttatgcca tctgtcagtt agccaaacag
     6601 gcacgcagtt ggcgcccggc gctggtgcaa atactcaagc aggtgcgcaa gaccatcatt
     6661 tatgacaatg tggctgtgta tttgctggac cgcaacaccg gcagcctgga ggtcagctac
     6721 gccaagtcgg tggggcgcgg gcgctcgtct gaggccgatg tggcctgggg ggaagtggcg
     6781 gccgggctgg tttttcagac cggggataag gtgctgcaag agcccgaacc caaccccaac
     6841 cttgaccggc tggagcagcc ctttatttta ggcatgccgc tgaaactgga gcaggagatt
     6901 ctgggagcga tggtgttcat ccgctttggc gggccgcact atacgccgga gcaaattgaa
     6961 tacgccagct ttacggcgca gctggctggc tgtatgattg gcgtgcagga actaaagcag
     7021 cagctcagca cggttgaaac cctgaagcag atgctggata ttcagggtga tttgatctcc
     7081 attctttcgc atgaaattcg caacccactg ggctttatca aagggtacac ctccaccctg
     7141 ctgcgttctg atacggaatg ggatgcccag acccagcgcg agtttttgac aattattgag
     7201 agtgaaacag accggctgga acgcctgctg gagaatttgc tggactctgc ccggcttgaa
     7261 tcgggacaga cgattatcaa attcctgccg gtgcggctgg aagcgctgtt taacgacatg
     7321 aaggaacgcg ccaaactgca gtacccagac atgcagatag aggtgcagat tgaaacctcg
     7381 ccggcaactg tgctgggaga tccccggcgg ttgacccagg tgtttgaaaa cctgttgagc
     7441 aactcggcca agtatgcgcc gggatcgccg gtgcatatca acgttcagca aatggatcag
     7501 cctaaaggga ttctgatccg cttttcggac agcgggccgg gcattccaga gaagcatttg
     7561 cccttcattt ttgagcggtt cttccgaaac ccggaacaga atttacagat ttacgggaca
     7621 gggctgggat tgtatattgc gcggcagatt gtgctggcgc atcacggacg gatcacggcg
     7681 acatctgacg caggcaaagg tgttactttt cacatcttcc tgcccgaaca aacctttgaa
     7741 cccccggtgg cttaagcatg agcgcattaa ttctggttgt tgatgacgaa gccctgtacc
     7801 aacggctgat ccgcgtcaat ctggaggctg aagggtatca ggtgaatacc gctggcgatg
     7861 gggaagcggg tttggaagcc attgcccaaa agatgcccga cctggtgatt ctggacgtga
     7921 tgatgccaaa gctggatggc tttaccacct gcgagcgtat tcgccagttt tcgagtgtgc
     7981 caattattat gctgacggct aaagccgaag aggaagaccg ggtgcgaggg ctgaatgcag
     8041 gtgcagatga ttacattgcc aaacctttct cggcgaccga actggtggcg cgggtgcggg
     8101 cggtgctgcg ccgggcgcaa agagcggatc aatttacaca gaatcggtat ttcactcatg
     8161 gcaacctgcg gattgatttt gccagggctg aaatctggaa agatgataaa gcgatctatc
     8221 tgtcggcaac agaatatcgg ttgttgattc aatttgccca gcatgttggg caggtgctga
     8281 cggcggagga cttgctgctt tcggtgtggg gcgagcaata ccgcgaagat aaagagattt
     8341 tatgggtgag tatagcccga ctgcggcaga agctggaaga taatccgcac agcccgcagc
     8401 atattgtaac ccgggctggt ctgggctatt tgatgccccc ttcagacagt aacgcctgat
     8461 ttcaagtgag gagtaaacca tggtgacttc ttttgacgag aagggcaaga tttttaccca
     8521 ggtagtgcgc aagcagccaa cgcaggtcac aatccagacc ctggattatc agatccgggg
     8581 gcaggtgcat gtgcatccgg acagccggtt gaaagatgag attgaacgcg aggagcattt
     8641 tgtggcgctc acggatgtaa ccctccacga taaaagtgga gcgctggtta tgcgcacagc
     8701 ctttattgct gttaataagg cgcagatcat ctggattgtg ccggatgaag atattgccaa
     8761 ggaaaaacaa gaatgagccc actgcaaacc actcaaacac cccaggctgc caaaccgcct
     8821 gaactgctcc ggctgaaaga gcatctgatc agcctgattt ctcgcgagct ggaaatgcgc
     8881 agcccgccga tgaatgaacg ccgggaggcg attcaacaaa ttatggcttc ggcatatcaa
     8941 gggctgcgat tatcgctgcc caatacgctg cgagatcagc tcttccatga gattatggat
     9001 gacttgatgg gttttgggcc gttacaaccc ctgctggacg acccggacat caccgaagtg
     9061 atggtgaatg ggccgaagaa ggtctatgtt gagcggaagg gcaagattac caagaccaat
     9121 gtgacatttg atgacgacaa cgcggtcatc cgggtgattg agaagatcat cctgccgttg
     9181 gggcgccgaa ttgatggcga cagcccaacc gtggatgccc gcctgcccga cgggtcgcgc
     9241 gtgaa
//
LOCUS       DAOPVS010000076         9232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7526_length_9232_cov_54.805601, whole genome shotgun sequence.
ACCESSION   DAOPVS010000076 DAOPVS010000000
VERSION     DAOPVS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7526_length_9232_cov_54.805601"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1221)
                     /locus_tag="PKL11_07235"
     CDS             complement(97..1221)
                     /locus_tag="PKL11_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13435.1"
                     /translation="MSIEIREVRSAKELNAFIRFPHTLYRGHPCWVPLLSMDERNTFR
                     QDKNPAFEHCQARYWLAYRDGKIVGRIAGILNNRHIEKWNQNYLRFSWIDFVDDLEVS
                     KALLETVEAWGREIGLTAVHGPLGFTDMDREGMLVEGFDEMGTMATFYNHPYYPVHLQ
                     QHGYIKDIDWVEYEITIPPEPIEEISRMAEVVLRRNNLHVFQPRNKRELLGYAQSLFE
                     LLNAEYAHLYGTVMLNQRQIEAYTKEYFSFVSPDLLPIVLDAQNQMVAFGIAMPSLSR
                     ALQKTRGRLLPFGIIHLLWALSHNQRADLYLVAIKREYQGKGVNAVLMSQIHKAFIAR
                     GIQKAESNPELENNLRVQTQWKYYETRQHKRRRIFIKQLN"
     gene            complement(1342..2109)
                     /locus_tag="PKL11_07240"
     CDS             complement(1342..2109)
                     /locus_tag="PKL11_07240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13436.1"
                     /translation="MINYPLTRANRIRLARAFFNARRVDLSIDCVLEDQMGQAWVDCL
                     EDPTVFQIQTGPFVYFAGDLHSAAAREMIAGLPPFTLVMPTLPGWLERVQEIHGERFE
                     LYERASFSGESITVEHLNKLIASSPWQDNLRPMEADFIRPIWGQDHFIDLAMFDSIED
                     FEARGIGFYVRIHGDLAGAAFSQLVCSQGIEVSLFVLPEYRRQGVATLLSASLIKACR
                     NRGQKAHWDAANPESGRLAVKLGYLPAGTYEGGYLGE"
     gene            complement(2156..3583)
                     /locus_tag="PKL11_07245"
     CDS             complement(2156..3583)
                     /locus_tag="PKL11_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ13437.1"
                     /translation="MSIRLRFTLLYNAILAVTLAVFGISLYSIQARTTMDSLKRDLAH
                     SSEALGGPLVRVTISFPIMDQMQYHDSPMPFENFSDEPAFKKLPEREIVRVLDADGYL
                     VASPFGAAEDALPLSPEGLAALTKGQEWWEIATVNERLLIYNQPIMAGEHLQFILQIA
                     RPLTERDRTLKALGSTLIFASLVTLAAAFGIGWLLSGITLRPIHRITQTAMEIGLARD
                     FKQRVNYAGPQDEVGQLATTFNGMLARLDEAYQRVAHSLEMQRNFVADVSHELRTPLT
                     TLRGNLGLLRRTPPIPAEEQADILTDMVDESDRMIRLVNDLLVLARADAGRNLTHEPL
                     NLTPILEEVGRQARQLDSMRTITLDMPPEVEILGDRDAFKQVVLILVDNALKHSQGAV
                     EITARQQDRQVVVQVQDHGEGMTPEKLAHVFDRFYRGEDATTPGFGLGLSIAKSLVEG
                     MGGTIGIESEIGKGSLVQLRFPPAE"
     gene            complement(3583..4260)
                     /locus_tag="PKL11_07250"
     CDS             complement(3583..4260)
                     /locus_tag="PKL11_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13438.1"
                     /translation="MPTVLIVDDDQKLLKMLQRTLTYENLNALTAANGVEALQVVNAQ
                     QPDLLIVDWMMPQMDGLTLIQRLRDGGNDVPILMLTARDAIENRVDGLESGADDYLIK
                     PFAPAELVARVHALLRRIEPKVDTRPATYADIRLDPITHQAWRGERALTLTLTEFNLL
                     QLLLRHPRQVLERGQILTEVWGYDFGGDSNVLEIYVSYLRKKLEEQGESRLIHTVRGV
                     GYVLREE"
     gene            complement(4428..5042)
                     /locus_tag="PKL11_07255"
     CDS             complement(4428..5042)
                     /locus_tag="PKL11_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13439.1"
                     /translation="MITCPWCGTSYVAFQSNCKNCGGPLLPPTPVESQVESELPAPPP
                     PPRAISTAYTWKLMSTDAGSIGGFVVMMVGFIFGIVGVGLTAGIITAFVGIPFAVLGL
                     AMLVGGAGMVYWRYQEKHKIVAVLQTGQTARGQIIAVDENMAVSINNRHPWNIRYGFI
                     YNGQNWEGSTTTLNTPGPNLQPGQATWVLYMPESPELNAIFPHP"
     gene            complement(5039..5464)
                     /locus_tag="PKL11_07260"
     CDS             complement(5039..5464)
                     /locus_tag="PKL11_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNZ13440.1"
                     /translation="MEPILTRCGYRCDLCLAYRPNIDANPENRQKLSDGWFAYFGFRI
                     PPEEIYCDGCMTLEPQKLIDTACPVRPCVVARGLAHCAECEVIQCEKLAERLVTREAV
                     EQRIGRTAPEADYQDFIRPYENKPRLDALRASRSKENKA"
     gene            complement(5468..5854)
                     /locus_tag="PKL11_07265"
     CDS             complement(5468..5854)
                     /locus_tag="PKL11_07265"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNZ13441.1"
                     /translation="MLTERIFLEHFSYVPEHLLEIAGEVRNLVAQTAPQATEVLRKNG
                     AVYFDAARGGPVKAGICQIRILSDHIRVVFAHGAFLPDRQGLLVGDQLAMRHLPIYNY
                     EAAPWDALLALIEASYHFDPCSLAQG"
     gene            complement(5866..6285)
                     /locus_tag="PKL11_07270"
     CDS             complement(5866..6285)
                     /locus_tag="PKL11_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13442.1"
                     /translation="MDNLEPQGILPRIDLKRLWKIGVALVTGAIFPVFMLFIFGFSIK
                     TTPEYACVMQMVESNRQVVNVTGEPVTPGFFAWTGFFESGGGVRQGSFSTRVSGPQGR
                     VTIQAQFYRTPIGETLGIWLKVKGDELTLYNGDYPCR"
     gene            6432..7220
                     /locus_tag="PKL11_07275"
     CDS             6432..7220
                     /locus_tag="PKL11_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13443.1"
                     /translation="MFNRSLKTASFLLIGIGVLLLVLNILLDGTLNIALPLVFLMLGG
                     ILFMMVYAARPKWAGSSILYIPGSLLLAFGLVFLLNVVTKDWASWAYAWLLLLAGIGV
                     GMVLAYQHQTWPPLVNLIGWGLAIAGVTFFAVFGAIAGGVFIQVMAPILIVLGGVILR
                     WLHPERILPEHLLKRLGAQPLSDAEAPASAAETASLVEPLSTRELEVLHLVDQGLSNQ
                     EIAAQLHIAASTVKTHINNIYGKLGVQTRVQALNRARELGLLNA"
     gene            7379..8278
                     /locus_tag="PKL11_07280"
     CDS             7379..8278
                     /locus_tag="PKL11_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ13444.1"
                     /translation="METDLSDKTFVVTGATSGIGFAVASALSARGAAVIGVGRSTERC
                     RLAAHQLRSLNPKSQVDYLSADLSIQSEVRRLAGQIQTLLAARDKTALDGLLNNAGTY
                     AYWLTLTPDGVELQWAVNHLAPFLLTCQLLPLLQAAPCARVVTVSSASHYGARINWSD
                     PQLRRRYDGLQAYGNTKLANVLFTQALNQRLGTASTVRAFAADPGLVKTDIALKGTPP
                     IVNWFWKIRRSGGISPAETAVGIVFLLTEPGIQNSPACYWKHSHPRRPSRPARDLASA
                     DRLWALSEKMCGFHLEVNHDTVC"
     gene            8262..9107
                     /locus_tag="PKL11_07285"
     CDS             8262..9107
                     /locus_tag="PKL11_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ13445.1"
                     /translation="MTQFADRSAHRSYVAITGAGGGLGKAFAVECASRGWDLFLTDLS
                     PAPLEQLAVSLYNAYSIDVVVYPCDLTDAVARTNLFETIRSTGLRFWALINVAGLDYE
                     GLFYQQTSQQIRTIIRLNIEGTLDMIHSLLEYRDPLQVFRIINVASLAAFYPMPVKAT
                     YAASKRFLLDFSRALRQEVRGLGATVTVLCPAGMPTTSACIQAIRAQGWMGQITTCDV
                     GRVANQTLNAALAGRGLVIPGLINRMLQTLGALVPAPLVAALIGSRWKSAHQKRALDQ
                     RLAES"
BASE COUNT         1985 a         2817 c         2313 g         2117 t
ORIGIN      
        1 ttaccacact ttcctgcccc tgccgaaacg atttgcccaa tctttgagaa gactaatctc
       61 tttcgagagc ataaaagcag tcaaaaacct gacttattaa ttcagttgct tgataaagat
      121 ccgccgccgt ttgtgctgcc gggtctcata atacttccac tgggtttgca cccgtagatt
      181 attctccagt tccgggttgg attccgcctt ctggatgccg cgcgcaataa aggccttgtg
      241 aatctggctc atcagcaccg cattcacgcc cttaccctga tattcccgct taatcgccac
      301 cagataaagg tcagcgcgtt gattatggct taacgcccac agcagatgga tgattccaaa
      361 aggcagcagt cggccgcgcg ttttttgcag cgcccggctc agtgatggca tggcaattcc
      421 aaacgccacc atctgatttt gtgcatccag cacaatcggc agcaaatctg gcgaaacgaa
      481 gctgaaatac tctttggtat aggcctcaat ttgtcgttgg ttaagcatca cggtgccgta
      541 aaggtgggca tattcagcat tcagcaattc aaacagagat tgggcgtaac ccagcagctc
      601 acgtttgttg cgtggctgaa agacatgcag attgttgcgg cgcagtacaa cctcagccat
      661 tcgggatatt tcctcaatcg gttcgggcgg aatggtaatc tcgtactcaa cccagtcaat
      721 atccttgata tacccatgct gctgcaaatg cacaggataa taggggtgat tataaaaggt
      781 tgccatcgtc cccatttcat caaacccttc caccagcatc ccctcacgat ccatatcggt
      841 gaatcccagc ggaccatgca ctgctgtcag cccaatctcc cgcccccagg cttccacggt
      901 ttccaacagc gccttagaga cttccagatc gtcaacgaaa tcaatccagc taaaccgcag
      961 gtagttctga ttccactttt cgatatgccg gttgttcaga atgcccgcaa tccgcccaac
     1021 aatctttccg tcccggtaag ccagccagta ccgtgcctgg cagtgttcaa aagccgggtt
     1081 cttatcctgc cggaaggtat tgcgctcatc catcgagagc agcggcaccc agcaggggtg
     1141 cccccgatac agcgtgtgtg gaaaacgaat aaaagcattc agctctttcg cactgcgaac
     1201 ttccctgatt tcaatactca attgaataac ctcaaagggc agaataattg ccccctgatt
     1261 atatccgata tccgtaattg ctcagctgag attatcgcat ttattttggg tgatcatcac
     1321 ttacccaatc gccagattgc ctcattcacc cagatagccc ccctcatagg ttccggcagg
     1381 cagatagccc agcttgaccg ccagcctccc tgattctgga ttggctgcat cccagtgcgc
     1441 tttttgtccc cggttccggc aggcttttat caggctggcc gaaagcagcg ttgccacacc
     1501 ttggcggcga tactctggca gcacaaacag actcacttca attccctggc tgcaaaccag
     1561 ctgcgaaaat gctgcgccag ccaggtcgcc atggatgcgg acgtaaaagc caattccgcg
     1621 ggcctcaaag tcttcaattg aatcaaacat tgccaggtca ataaaatgat cctgccccca
     1681 gataggccgg ataaaatccg cctccattgg gcgcaggtta tcctgccagg gtgaactggc
     1741 aatcaattta tttaaatgct caacagtgat gctttcgccc gaaaagctcg ctcgttcata
     1801 caattcaaat ctttcgccgt gaatttcctg gacccgttcc agccaccctg ggagcgtcgg
     1861 catcaccagg gtaaagggcg gcagtccggc aatcatctct cgcgcggcgg cgctgtgcag
     1921 gtcaccggca aagtacacaa acggcccggt ttgaatttga aaaactgtgg ggtcctcaag
     1981 acagtccacc caggcctgtc ccatttgatc ttccagcacg cagtcaattg aaagatccac
     2041 ccgccgagca ttgaaaaatg cccgggcaag tctgatgcgg ttggcccgcg tgaggggata
     2101 attaatcatc tggacagctc cttgggatcc cccttattgt actctgaaat gctgatcatt
     2161 ctgcaggtgg aaaacgcaac tgcaccaggc tgccctttcc aatctcactt tcaatgccaa
     2221 tggttccccc cataccctcc accagcgatt ttgcaatgga cagccccagc ccaaagcccg
     2281 gtgttgtggc atcctcgccg cggtaaaacc ggtcaaacac atgcgccagt ttctccggcg
     2341 tcatcccctc gccgtgatct tgcacctgta ccaccacctg ccggtcctgc tgccgcgctg
     2401 taatctccac cgccccctgt gaatgtttga gcgcattatc taccaggatc aagaccacct
     2461 gcttgaatgc gtcgcggtct cccagaattt ccacctctgg cggcatatcc agcgtaatcg
     2521 tacgcatgga gtcaagctgc cgggcctgcc gaccaacttc ttccagtatc ggtgtcaggt
     2581 tcagcggctc atgggtcagg ttccgcccgg catcggcacg cgccagcacc agcagatcgt
     2641 tcaccagacg gatcatccgg tcgctttcgt caaccatatc agtcagaatg tcggcctgct
     2701 cttctgccgg aatgggcggt gtgcgccgca gcagccccag gttgccccgc agtgtggtca
     2761 gcggggtgcg taattcatgc gatacatccg ccacaaaatt gcgctgcatt tccagtgagt
     2821 gcgccacgcg ttggtaggct tcatccaggc gagccagcat gccattaaag gtagtggcta
     2881 actgccccac ctcatcctgc ggtccggcat aattgacccg ctgcttaaaa tcgcgcgcca
     2941 gcccaatctc catcgccgtc tgagtaatgc ggtgaatagg ccgcaatgtg attcccgaca
     3001 gcaaccatcc gatcccaaaa gccgcagcca gcgtcaccaa actggcaaaa atcagcgtac
     3061 tgcccagtgc tttcagtgtc cggtctcgct ctgtcagcgg gcgtgcaatt tgcaaaatga
     3121 attgtaaatg ctccccggcc atgatcggct ggttgtaaat caacaaccgc tcgttaacgg
     3181 tggcaatctc ccaccactcc tggcccttgg tcagggctgc caggccctca gggctcaacg
     3241 gcagggcgtc ttcagccgcc ccaaatggac tggcaaccag gtatccatcg gcatccagca
     3301 ctcggacaat ctcccgctct ggcagctttt tgaaagctgg ctcgtctgag aagttctcaa
     3361 acggcatggg cgaatcgtga tactgcatct gatccataat cggaaacgag atcgtcaccc
     3421 gcaccagtgg gccgcccagt gcttcactgc tgtgagccag gtcgcgcttc aggctgtcca
     3481 tggtggtgcg cgcctgaatg gaatacagtg aaatcccaaa gaccgccagt gtcaccgcca
     3541 gaatggcatt atagagcagg gtaaagcgta atcggatgga catcattcct cgcgcagcac
     3601 ataacccacc ccgcgcacgg tgtgaatgag ccgggattcg ccctgctctt ccagcttctt
     3661 gcgcaggtag ctgacgtaaa tttccaacac attgctgtcg ccgccaaaat cataccccca
     3721 aacctcagtc aaaatctgac cgcgctcaag cacctgccgg ggatgccgca gcagcaattg
     3781 cagcaggtta aattcggtca gggtcagggt cagggcgcgc tcaccccgcc aggcctgatg
     3841 cgtaatcggg tccaggcgta tatcagcata ggtggcgggc cgggtatcca ccttgggctc
     3901 aatccgccgc agcagcgcat gaacccgcgc caccagttct gccggggcaa aaggcttgat
     3961 caggtaatca tctgcgccgc tttccagccc gtccacccga ttttcaattg catcgcgcgc
     4021 ggtcagcatc agaatgggca catcgttacc cccatcgcgc agccgttgaa tcagtgtcag
     4081 tccgtccatt tgtggcatca tccaatccac aatcaataaa tcaggctgct gagcgttgac
     4141 cacctgcagt gcttctacgc cattggctgc cgtcagcgca ttcaagtttt cgtaagtcag
     4201 cgtgcgctgc agcattttca atagtttctg gtcatcatcc acaattaata ccgttggcat
     4261 cttttcacct gggcagatcg tctgatatag gtatatcaca atataagcca gtttgctgtg
     4321 aaaatgctgt gaaactagcg cttctcgcgt cttaaaaaca tccgcaaaca gggatgctcc
     4381 tggtttgggg tgctagatcg tcccttaaga aaatcgcgcc gggcagccta tggatgcgga
     4441 aaaatggcat ttagctctgg cgactctggc atataaagga cccaggtcgc ttgtcccggc
     4501 tgtaaatttg gcccaggcgt attcagcgtt gtggtgctgc cttcccagtt ctgcccatta
     4561 taaatgaacc cgtaccggat attccaggga tgccggttat tgattgaaac cgccatgttt
     4621 tcgtccacgg caataatctg cccgcgtgcg gtttgccctg tctgcagcac agccacaatt
     4681 ttgtgcttct cctggtatcg ccaatacacc atgcctgcgc cgcccaccaa catcgccagc
     4741 cccagcactg caaaggggat gcccacgaac gcagtgatga tccccgccgt caggcccaca
     4801 ccaacgattc cgaaaatgaa ccccaccatc atcaccacaa atccgccaat tgacccggca
     4861 tccgttgaca tcaacttcca ggtataggcc gttgaaatgg cgcgcggggg tggtggggga
     4921 gctggcagct cagattcaac ctgactttct accggtgttg gcggcaacag cggtcccccg
     4981 cagttcttgc agttggattg aaatgctaca tagctcgtgc cgcaccatgg acaggtaatc
     5041 atgccttgtt ctcctttgac cggctggcgc gcagcgcatc cagcctgggt ttgttttcgt
     5101 atgggcgaat gaaatcctga taatcggctt ccggcgcggt ccggccaatc cgctgttcca
     5161 ccgcttcccg cgtgaccagc cgctctgcca gtttctcgca ctgaatcacc tcgcattcgg
     5221 cgcagtgcgc cagccctctt gcaaccacac agggcctcac cgggcaggcc gtatcaatca
     5281 atttttgcgg ctccagggtc atacacccgt cgcaataaat ctcctccggc gggatgcgaa
     5341 agccaaaata ggcaaaccac ccatcactca gcttttgccg gttctccggg ttggcatcaa
     5401 tattagggcg gtaggccagg cataaatcac agcgatagcc gcagcgggtc aagataggtt
     5461 ccatcatcta accttgggcc agtgaacagg gatcaaaatg gtaggatgcc tcaatcaaag
     5521 caagcaaagc atcccagggt gcagcttcgt aattataaat cggcaggtga cgcattgcca
     5581 gttgatctcc caccagcagc ccctgacggt ctggcaggaa tgccccgtgt gcaaaaacca
     5641 cccggatatg gtctgacaga atgcgaatct ggcaaatgcc cgccttcacg gggccgcccc
     5701 gtgccgcatc aaaatagacc gcgccatttt ttcgaagcac ctcggtggcc tggggcgcag
     5761 tctgcgccac caggttgcgg acttcacctg caatttccag caggtgttcc ggcacataac
     5821 taaaatgctc cagaaatata cgctcagtca gcatggttgc aggctttatc ggcaggggta
     5881 atccccattg tagagcgtca gttcatcccc cttaaccttg agccagatcc ccagcgtttc
     5941 gccaatgggc gtgcggtaaa actgcgcttg aatggtcacc cggccttgtg gccctgagac
     6001 gcgcgtggaa aaactgccct gccgcacccc gcccccgctt tcaaagaaac ctgtccaggc
     6061 aaagaacccg ggtgtcaccg gctccccggt cacgttgaca acctgccgat tgctttcaac
     6121 catttgcatc acgcaggcat attcaggggt ggtcttaatc gaaaacccaa agatgaacag
     6181 cataaagaca gggaaaatgg cccccgtaac gagcgccacc ccaattttcc agagccgctt
     6241 caggtcaatt cgcggcagaa taccctgagg ttcaagatta tccataaaaa gctccagcaa
     6301 tcatagaatg agttctgatt tgattatagg atgattatcg cgcaggtcaa gcgcttttaa
     6361 ctcccctcta tataaattgg gcctcggtcc gggaagcggt ataattgacc catcaggaga
     6421 cccttttccc tatgtttaat cgctccctca aaacagccag cttcctcttg attggcattg
     6481 gagtcttgct gctggtgctg aatattctac tggacggcac attgaacatc gccttgccgc
     6541 tggtctttct catgttgggt ggcatcctct ttatgatggt ttatgcggcg cgccccaaat
     6601 gggccggatc gtccatcctc tatatacccg gctcgctgct gctggccttt gggttggtct
     6661 tcctgctcaa cgtggtgacc aaagattggg cctcctgggc ttatgcctgg ctgctgctgc
     6721 tggctggcat tggggtgggc atggtgctgg cctaccaaca tcagacctgg ccgccgctgg
     6781 tcaacctgat tggctggggg ctggccattg ccggtgtgac cttttttgcc gtttttggcg
     6841 ccattgctgg cggggtgttt attcaggtga tggcgcccat cctcattgtt ctggggggcg
     6901 tcattttgcg ctggctgcat cccgaaagaa tcctgccgga gcacctgctc aagcgcctgg
     6961 gtgcccagcc gctctcagat gctgaagccc ccgcgtccgc agccgaaacc gcctccttgg
     7021 ttgaacccct gtccacccgc gaactggaag tcctccacct ggtggatcaa ggcttatcca
     7081 atcaagaaat tgctgcccaa ctgcacattg ccgccagtac cgtcaaaacc cacattaata
     7141 atatttacgg caaactcggc gtgcagaccc gtgttcaggc cctcaaccgg gcgcgtgagt
     7201 taggcctgct caacgcctga tgcttgcccg ctgaacttca ggcgcagacc cctgcgcctg
     7261 tttttatttt tccaccccct ccatccaccc gcattccacc ctttggcaga tgtcactcca
     7321 cccgctcaac gggtatacag gaagtgcaat tgcaaattct ctgccacagg aagcaaccat
     7381 ggaaaccgat ttatctgata aaacctttgt cgtcaccggt gccacctccg gcatcggctt
     7441 tgcggtggcc tcggctctaa gcgcccgggg cgcggcggtg attggggtag gccgctcaac
     7501 cgagcgctgc cgcctggccg ctcaccagtt acgctccctc aatcccaaat cccaggtgga
     7561 ttacctttcc gccgatttga gcattcaaag tgaggtccgg cgcctcgccg ggcagattca
     7621 gaccttgctg gccgcccggg acaaaactgc actggatggc ctgctgaata atgccggtac
     7681 ctatgcttac tggctgacgt taacgcccga tggtgtggaa ttgcagtggg cggtcaatca
     7741 tctggctcca tttctgctga cttgccagct gctgcccttg cttcaggccg ctccctgcgc
     7801 ccgggtggtt accgtaagct ccgcctctca ttatggtgcc cgcatcaatt ggtccgatcc
     7861 gcagctccgc cgccgctatg acggactgca ggcctacggc aataccaagt tggctaacgt
     7921 acttttcacc caggcgctca accagcgttt gggcaccgca tccactgtcc gggcatttgc
     7981 cgctgacccc ggcctggtga aaaccgatat tgcgctcaaa ggcacaccgc ccatcgtgaa
     8041 ctggttctgg aagattcgcc gctctggcgg tatctcgccg gctgagacag cggttggcat
     8101 cgtctttctc ctgaccgagc ccggcattca aaattctccc gcctgttatt ggaaacacag
     8161 ccatccccgg cggcccagcc gtcccgcgcg tgatctggcc tcggcagatc gcctgtgggc
     8221 tttatccgaa aaaatgtgtg gttttcattt agaggtgaat catgacacag tttgctgatc
     8281 gttctgccca ccggagttat gtcgccatca ccggggcagg gggtggcctg ggcaaggctt
     8341 ttgccgttga gtgtgccagc cgcggctggg acctgttcct tacggacctg tcacctgcgc
     8401 cgttggaaca gttagccgtc agcctgtata atgcgtatag cattgatgtt gtcgtttatc
     8461 cctgcgatct gaccgacgct gttgcccgca cgaacctttt tgaaacgatc cgttccaccg
     8521 ggctgcgctt ctgggcgctc atcaatgttg ccggcctgga ctacgaaggc ctcttttacc
     8581 agcagaccag ccagcaaatc cgcaccatca tccgtcttaa cattgaaggc acgctggaca
     8641 tgatccattc cctgctggaa taccgtgatc cgctccaggt ctttcgcatc atcaatgttg
     8701 ccagcctggc tgccttttac cccatgccgg ttaaggccac ttatgccgcc tctaaacgct
     8761 tcctgctcga tttctcgcgc gccctacgcc aggaagtccg cggcctgggg gctacggtca
     8821 cggtgctctg tccggcgggc atgccaacca cttcagcttg cattcaggcc atccgagccc
     8881 agggctggat gggtcaaatc accacctgtg atgtaggccg ggtagccaat cagacgctga
     8941 atgctgctct ggcagggcga ggattggtca tccccggttt gatcaaccgg atgctccaaa
     9001 ccctgggtgc cctggtgccg gccccgttgg tagctgccct gattggcagc cgctggaaat
     9061 cagctcacca gaagcgcgcg cttgaccaac gcctggcaga aagctagacc acgataccgc
     9121 cggttacaat aaacttgagg cgccctccag attgggaggg cgcctcgttt gttgatcaca
     9181 acttctgcgc cgtcacaaac cagttatgcg gaacattagg catttcggtt gg
//
LOCUS       DAOPVS010000077         9203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7546_length_9203_cov_54.812855, whole genome shotgun sequence.
ACCESSION   DAOPVS010000077 DAOPVS010000000
VERSION     DAOPVS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7546_length_9203_cov_54.812855"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKL11_07290"
     CDS             complement(<1..477)
                     /locus_tag="PKL11_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ13446.1"
                     /translation="MDSPLIPTALNTPDYRQLLQQCVHCGMCLSACPTYAVFGTEMDG
                     PRGRITLMRSAAEGRLSQTELLGQFSQHINLCLACLSCQTACPSGVQYARLLEGAHQA
                     IAQYHRPGLVERLVRWLGLGQLMPHVGRLRLMSTLFWFYQHLGLQWLVRKLDFLPQP"
     gene            597..1235
                     /locus_tag="PKL11_07295"
     CDS             597..1235
                     /locus_tag="PKL11_07295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNZ13447.1"
                     /translation="MTSAHPRGGIPLAKRILDLALTLPAMLILLPAMGIIALLVWLMD
                     GRPVFFRQPRPGLHGKIFTLTKFRTMRLDLDPHGAPLPDAQRLTRLGRFLRATSLDEL
                     PEFFLVISGKMSLVGPRPLLTAYLERYSPEQARRHDVLPGITGWAQVNGRNALSWEDK
                     FRLDVWYVDHWSVGLDLKIIALTLWKAIKREGISQPGQATAEEFKGTPREQG"
     gene            complement(1258..>1839)
                     /locus_tag="PKL11_07300"
     CDS             complement(1258..>1839)
                     /locus_tag="PKL11_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13448.1"
                     /translation="ADAQTFIGGMISTLIAPDWRGGALLPTDGPLGDTVSSALVNGGR
                     YYCGRCAPYYAPVVLFPVTTTLPSISDAAAWQAAANALHEQNRLEVIYLAPEAVSPEL
                     ASALSSQFILVGGVAPLGGVPANWAATVTWDVATALQMLWPAVSANNAGQALDAPLAL
                     ADINPTLLSPGRQALVQEAIDLLAAGQLGVLTP"
     assembly_gap    1840..1939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2029..3237
                     /locus_tag="PKL11_07305"
     CDS             2029..3237
                     /locus_tag="PKL11_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNZ13449.1"
                     /translation="MSLPTSQPILPEPEPQLPPARRRQQQRMLPSNAGSAERREFLAE
                     VSQRVTPSLDFFLFSLLAGFVMALALLLDSPALYVLVILLAPFMSPVLGAALGTVMGS
                     VRFFMRSVAAVLIGALIFFIWGLAAGIIYRLLPAYPIQQAMDHSVFSWPDLIVLALGA
                     ILAAYLIIKAPRQRPLVASVALAYEILLPVGMSGFGLTSGIGGLFPDGLLVFAVHLVA
                     AILLCTVVVIALGFRPRNVLGYGLAALIVLGCVVAAMLLTGGIAMPLPPTPTPTVTPT
                     LPTATPTITPVPPTPTQTRTPTATATPTRTPTLMPTETPTLTITPQPTPVWAFIYAKD
                     GNGAYIRAEPGFDSARIASLQNEHLVQVISDPVFKDNYLWVQIRIPEDGTEGWIVQSL
                     LQTATPSTGW"
     assembly_gap    3397..3496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3497..4045
                     /locus_tag="PKL11_07310"
     CDS             <3497..4045
                     /locus_tag="PKL11_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNZ13450.1"
                     /translation="VSTTRMIDRLGERYGLPVYETPVGFNHIADYMMKEDILIGGEES
                     GGISFKGHIPEGDGVIMGLLIIEMVASAGVTLYELVEDLLNEVGPAYYERTDLRLRHP
                     VSKSQMTDRLKNEVPSAIGGERIAQISDKDGVKYILADDSWLLIRPSGTEPVLRVYAE
                     GRSPQMVKALLAYGESVAASVS"
     gene            complement(4184..4834)
                     /locus_tag="PKL11_07315"
     CDS             complement(4184..4834)
                     /locus_tag="PKL11_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13451.1"
                     /translation="MAFQGFDEEEEGNQFADLLPQEEPPAAPEAEEPEPRQGGGGGNN
                     RTFMLILAGVGGLILLMLIGLGVWYFVLRPQANNAIAQQNELVNATNTAMALNATTTA
                     QANIQYLTLAAIPTATKTPTKTPVVAPTTTLRPSSTPILNPTQQTQTVAAFNTQAAAT
                     QTAVVVLGTGTPRVPSPTALGGFGFADEVGLPGLLGLAAVLVIIIVVARKLRSSSR"
     gene            complement(4853..5905)
                     /locus_tag="PKL11_07320"
     CDS             complement(4853..5905)
                     /locus_tag="PKL11_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13452.1"
                     /translation="MVAPVNHILPVALIRRQRSLPLPGKVLVRMGQKVNATDPVAEAQ
                     LPGQHALFNIRRLLGGGRGGDQADRFIEVKVGARLMKGDVIAEAGGLFPRIVRAPANG
                     QVVAIVSGQVLLEMEPRIEQIYAGLNGTVTETTPDLGCTIEASGALIQGVWGNGQVGL
                     GLLLALAKEPLDEVTRASLDVSMRGAVVLGGYCNKVDAIEAGNELPLRGLILGSMSAD
                     LIPAARAARYPIVLTEGFGPIPMNSAAFKLLSTSERRDLCVNAIYDPVKGERPEIIIP
                     LPATGQLPMELISFKPGQMVHIWGAPYAGKIGTLLQVRPGLSALPNGVRAAAADVRLE
                     NNEPVVVPLANLDVIE"
     gene            complement(5930..>6871)
                     /locus_tag="PKL11_07325"
     CDS             complement(5930..>6871)
                     /locus_tag="PKL11_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNZ13453.1"
                     /translation="AQAFGRMIGYLSKIYDPQKGVLGVDLGQTYSTIAAGVGGQTALR
                     TFPLGMGAGIQSTLADLNIEDVSRWLPVPIGDDVLRDYLWQRSLRPGMVPVTVEALAM
                     EQAAARHILTLMMQGLQTRWPDLHNAFEPILAAGSVLSQWSSPGQSLLTILDGLQPVG
                     ITTVLLDAYGLLGSLGSISSHNALMPVQVLESNAFINLGTVICPLSEGRYGTPLLKVR
                     IEYESGEETQLEVRQGMLTALPIRNGQTARIHFSPLRRVVIDPNRKDRIRSFKVVGGL
                     CGAVIDTRGRPLVLPRDAARRRDMIRKWQTALGGSVV"
     assembly_gap    6872..6971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6972..7442)
                     /locus_tag="PKL11_07330"
     CDS             complement(<6972..7442)
                     /locus_tag="PKL11_07330"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNZ13454.1"
                     /translation="MTTSLVDAESVLAVDLGSVLTRVLLFDTVDGQYRFIASGVAPST
                     AGAPYSDIGEGINQALYQLQEVTGRVLVDPEGRLIMPAQPDGSGLDRLTIVFSAGPEL
                     RVVTVGLLEDVSLESANRLVEMTYGQVVEQIGINDRRRPEVQVDAILQARPDLVL"
     gene            complement(7455..8120)
                     /locus_tag="PKL11_07335"
     CDS             complement(7455..8120)
                     /locus_tag="PKL11_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13455.1"
                     /translation="MGNLDLLWTLIGFLLTLMVFSYLFGDNPVFRLASYLLVGVGAGY
                     TAVVLIFEVIIPRLGAPLLSGDLTRQVLAGVPIILGLLLLTKLSPRLSSLGNVSMAYL
                     VGAGAGAAVGGAIFGTLFQQIGAAIAPFGMDGPNPFGQLLQGVLMVIGAAATLLYFHF
                     SARPRPDGAPRRSPLIETGAKIGEVFIGLTLGALFAGVMASALTALLERLNFLIQTIL
                     NLF"
     gene            complement(8125..>9203)
                     /locus_tag="PKL11_07340"
     CDS             complement(8125..>9203)
                     /locus_tag="PKL11_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13456.1"
                     /translation="GTVDDRIESAGPLAGIQGVLTSEELASRYLKPPTYSSRLRVTER
                     QRAHAELLESFIEEKSRPRGAQPETGLVPRAILRILIAAIMLVILLAPLLGGLQLTAL
                     PGASPEVSAFNAQMQQLTAAGESPRVLLAVDYEPGLAGEMQLISVGAVESLMQMNARI
                     ITLSTSPTGTALADQLLRTALRDIPAYPLVDQTISLGYLPGGESGLAQFANSPQQALP
                     YTTDGQSAWSLPVLEGAASLSDFDALLLLTDSPDVARSWIEQVQPSLGDVPLLVISSA
                     QAAPLILPYVQSNQVDGLVGGLYGSAAYDQLAQRPGSTARSLWDSYQIGMLLIVVFIL
                     VGGLYYAISRMLGRRKTRKPRREA"
BASE COUNT         1875 a         2537 c         2821 g         1670 t
ORIGIN      
        1 cggttggggt aaaaaatcga gcttgcgcac cagccactga agccccaggt gctgataaaa
       61 ccaaaacagg gtggacatca ggcgcaggcg gcccacgtgc ggcatcaact gccccaggcc
      121 caaccagcgc accagacgtt caaccagacc ggggcggtgg tactgtgcga tagcctggtg
      181 agcgccctct aacagccggg catattgaac cccggatgga caggcggtct gacaggacag
      241 gcaagccagg cacaggttga tatgctgcga aaactggccc agcaattcag tctggctcag
      301 gcggccttca gcggcagaac gcatcagcgt gatgcgcccg cgagggccat ccatttcagt
      361 tccaaagacc gcataggtcg ggcaggcact caggcacatg ccgcagtgaa cgcactgctg
      421 caaaagctgg cggtaatcag gggtgttcag ggcagtgggg attaagggag aatccatgca
      481 gagtccttcg atatccaaac aaaacaggat aaggcattgc atcccccagg gctgaaggcc
      541 cgacaaaatt atacctgatg cgagccaata accccctaat ctggtagaat gaggccatga
      601 cttctgctca cccccgcggc ggtatcccgc ttgccaaacg tattttggac ctggcgctga
      661 cgctgccagc catgctgatt ttgctgccag caatgggaat cattgccctg ctggtttggc
      721 tgatggatgg gcgcccggtc ttttttcgac agccacgccc ggggctgcat gggaagattt
      781 ttacactgac caagtttcga accatgcggc tggacctgga cccccacggc gcgcccctgc
      841 cagatgcaca gcggctgacc cggctgggac ggtttctgcg agccaccagc ctggacgaac
      901 tgcccgaatt ctttttagtc atcagcggta agatgagtct ggtggggccg cgtccgctgc
      961 taaccgccta cctggagcgt tacagcccgg agcaagcccg ccgtcacgat gtgctgccgg
     1021 gcatcaccgg ctgggcgcag gtgaatgggc gcaatgccct gagttgggaa gacaagtttc
     1081 ggctggatgt gtggtatgtc gatcactggt ccgtcgggct ggacctcaaa atcatcgcgc
     1141 taaccttatg gaaggccatc aagcgcgaag gcatcagcca gccgggacag gccacggcag
     1201 aggaattcaa gggaacgccg cgcgaacagg gctaggaagc ccggctctgg caggctgcta
     1261 gggtgtcaaa acgcccagtt gtccggcagc cagcagatcg atggcttcct gcaccagcgc
     1321 ctgacgcccg ggtgagagca gtgtggggtt gatatctgcc agggcaagcg gagcatcgag
     1381 cgcctgaccg gcgttattgg cgctgacagc gggccagagc atttgcagtg cagtggctac
     1441 atcccaggtg acggtggcgg cccagttggc cgggacgccg cccagcggcg ctacaccgcc
     1501 gactaatatg aactgactgg aaagagcaga agccagctca gggctgacag cctcaggcgc
     1561 caggtagatg acctccaggc ggttttgctc gtgcagggca ttggccgcgg cctgccaggc
     1621 agccgcatca ctgatggatg gaagggttgt ggtgacgggg aagagaacca ccggggcgta
     1681 gtagggcgcg cagcgcccgc agtaatagcg cccgccgttg accagagcac tagaaaccgt
     1741 atcgcccagg gggccatcgg taggcagcag ggcaccgcct cgccagtcgg gggcgatcag
     1801 ggtgctgatc atgccgccga taaaggtttg ggcatcggcn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnt aaacatatcc atagcattaa aaaaggtttg tggttcatcg
     1981 agtgtattat aattggaaga gaacggattt gaccatagtc ggatatttat gagtctgccc
     2041 accagtcaac ccattttacc ggaacccgaa ccgcaactgc cgcctgcccg ccgccggcag
     2101 cagcaacgca tgctgccctc aaatgccggg tcggcggagc gccgtgaatt tctggcggaa
     2161 gtttcgcaac gcgtaacacc ctcgctggat ttcttcctct tcagcctgct ggctggattt
     2221 gtgatggcgc tggcactgct gctggattca ccggcgctgt atgtgctggt gattttgctg
     2281 gcaccgttca tgtcgccggt gctgggtgcc gccctgggga cggtgatggg gtcggtgcga
     2341 tttttcatgc gctcggtggc ggctgtttta attggggcgc tgattttctt catctggggt
     2401 ctggccgccg ggatcatcta ccggctgctg ccggcttacc cgatccaaca ggcgatggac
     2461 cacagcgtat tttcctggcc tgatctgatt gtgctggcgc tgggggccat tctggcggct
     2521 tacctgatca tcaaggcgcc gcggcagcgc ccgctggtag ccagcgtggc gttagcttat
     2581 gaaattttgc tgcccgttgg gatgagtgga tttggcttaa ccagcgggat tggcgggtta
     2641 ttcccggacg ggctgctggt gtttgcggtt cacctcgttg ccgcgatact gctctgcacc
     2701 gtggtggtga ttgccctggg attccggccg cgcaatgtgc tgggatatgg actggcggcg
     2761 ctgattgtgc tgggctgcgt tgttgcagct atgctgctga ctggcggaat tgccatgccg
     2821 ctgccgccca cacccacgcc aaccgtgacg cccaccctgc cgacggcgac gccgaccatc
     2881 acgccggtgc cccccacccc aacccagacg cgcacgccaa ctgctacagc cacccccacc
     2941 cggacgccaa ccctgatgcc cacagagacc cccacgctga ccattacccc ccagccgaca
     3001 ccggtctggg cgtttatcta tgcgaaagac ggcaacggcg cttacatccg tgcggaaccc
     3061 ggattcgact cagcccggat tgccagcctg caaaatgagc atctggttca ggtgatatca
     3121 gatccggtct ttaaagacaa ttacttatgg gttcaaatcc ggattcctga agatggcacc
     3181 gagggctgga ttgtgcagag tttactgcaa acggcaacgc catcaaccgg ctggtaaaaa
     3241 tttgacgctt gttaacaagg agaattttgt atgaccattc actttggcac cgatggctgg
     3301 cgcgcggtca tctcagatac ttttacatat aataatctgc ggctggttac ccaggcgatt
     3361 gccgatgcgg tggcatcggg cgcctggatg aacggannnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnngtct cgactacccg gatgattgac cggctgggcg agcgctacgg
     3541 gctgcccgtc tatgaaactc cggtgggctt caaccatatt gccgactaca tgatgaaaga
     3601 agatattttg atcggcggcg aggaatcggg cggcatttcc ttcaaggggc atattccaga
     3661 aggcgatggc gtgattatgg ggctgctgat tattgagatg gtcgccagtg cgggggtcac
     3721 cctgtatgaa ctggtggaag atcttttgaa tgaagtgggg ccggcgtatt acgagcgcac
     3781 cgacctgcgg ctgcggcacc cggtatccaa gagtcagatg acagaccgtc tcaaaaatga
     3841 agtgccctcg gcaattggcg gcgagcggat cgcccagatc agcgataagg atggcgtaaa
     3901 gtatattctg gcagacgatt cgtggctgct gatccgacct tcggggacgg agccggtact
     3961 gcgggtttat gcggaggggc gctcaccgca gatggtgaaa gccctgctgg cttacggcga
     4021 atctgtggcg gcaagcgtct cgtaagctcc ccctttactg ctcaaagaca agaatcataa
     4081 ccgcagggcg aatttatcgc cctgcggttt tagcacatgg caatcaatac ctccggagat
     4141 ggccgcgctg ggcgccaagt ccgggcagac aggcaatggc ggtctagcgg gaagaagagc
     4201 gcagcttgcg agctacgacg atgatgatca ccagcaccgc tgccaggcca agcaagccag
     4261 gcagcccaac ctcatctgca aagccaaagc cgccgagagc ggtcggtgaa ggcacgcgcg
     4321 gcgtgccggt gccaagcacc accactgcgg tttgcgtggc tgccgcctga gtgttgaacg
     4381 ccgccacggt ttgggtttgc tgggtggggt tcaaaattgg ggtggaggac ggccgcaggg
     4441 ttgtggtggg agccacaaca ggcgtcttgg tgggggtttt ggttgcggtg gggatcgcgg
     4501 ccagagtcag atattgaata ttggcctgag ccgtggtggt ggcgttgagc gccatggcgg
     4561 tgttggtggc attgaccagc tcgttttgct gggcaatggc attattggcc tgcgggcgca
     4621 gcacaaaata ccagacgccc agaccaataa gcatcaacaa aatcaggccg ccaaccccag
     4681 ccagaatgag catgaaggtg cggttattgc cgccaccgcc gccctgacgg ggttctggct
     4741 cttcagcctc gggtgccgcc gggggttctt cctgcggcaa gagatcggca aattgattgc
     4801 cctcttcttc ttcgtcaaat ccttggaatg ccattgtgtt tctcctctgt gcttattcta
     4861 tcacatccag gttcgccagg ggtacaacca ccggctcgtt attttcaagg cggacatcgg
     4921 cagccgccgc gcgaacacca ttgggcaggg cgctcaaacc cgggcgcacc tgcagcaagg
     4981 ttccaatttt tccggcatag ggggcgcccc agatatgcac catctgaccg ggtttgaagc
     5041 tgattaattc cattggcagt tggccggtgg cgggcagggg gatgatgatt tcaggtcgtt
     5101 cacccttgac cgggtcgtaa atcgcgttga cgcacaggtc gcggcgttcg ctggtgctga
     5161 gcagcttgaa ggcggcgctg ttcatcggga tggggccaaa gccttcggtt aaaacgatgg
     5221 ggtagcgggc ggcgcgagct gcggggatca aatcagcaga catgctgccc aggatcagac
     5281 cgcgcaaggg cagttcgtta cccgcctcaa tggcgtccac tttattgcag tagccgccca
     5341 ggaccactgc gccgcgcata gaaacgtcca acgatgcgcg ggtcacctcg tccagaggct
     5401 ctttggctaa ggccagcagg aggcccaggc ccacctgacc attgccccag acgccctgga
     5461 tcaaggcgcc gctggcttca atggtgcagc ccaaatcggg agtggtttcg gtgacggttc
     5521 cattcagccc ggcataaatc tgctcaatcc ggggctccat ttcaagcaaa acctgaccgc
     5581 tgacaatggc aaccacctga ccattggccg gggcgcgtac aatgcgcggg aagaggccac
     5641 ccgcttcggc gatcacatcg cctttcatca ggcgggcgcc aaccttgact tcgataaagc
     5701 ggtcagcctg atcgccgccc cgcccaccgc ccagcaggcg gcggatatta aagagggcgt
     5761 gctgtccggg gagctgagct tcagccaccg ggtcggtggc attgactttc tgccccatgc
     5821 gaaccagaac cttgccgggc agcggcaggc tgcgctgtcg gcggatgaga gccacgggaa
     5881 gaatgtgatt aaccggagcg accatggatt tttatctccg atgcaagttt tataccactg
     5941 agccgccgag cgcggtttgc catttgcgaa tcatatcgcg acggcgagct gcatctcggg
     6001 gtaagaccag ggggcggccg cgggtatcaa tcacggcgcc gcagaggccg cccacaactt
     6061 tgaagctgcg gatgcggtct ttacgattag gatcaatgac gacccggcgg agcgggctga
     6121 agtgaatgcg ggccgtctga ccgttgcgaa tgggcagcgc ggtcaacatg ccctgccgga
     6181 cttccagttg ggtttcttcg cccgattcat actcgatccg cactttgagc agtggagtgc
     6241 cgtagcggcc ctcgctcagg gggcagatga cagttcccag attgatgaag gcgttgcttt
     6301 ccaacacctg gacgggcatc aaagcgttgt gggatgagat ggagcccaga gagccaagca
     6361 ggccgtaggc atccagcaaa acggtggtga tgccgactgg ctgcaagcca tccagaatgg
     6421 tcagcaggct ctgaccgggg gaggaccatt gagacaggac gctcccggcg gccaaaatgg
     6481 gctcaaaggc gttatgcagg tcaggccagc gggtctgcag accctgcatc atcaacgtca
     6541 ggatatgccg ggcagccgcc tgttccattg ccagggcctc aaccgtaacc gggaccatgc
     6601 ccgggcgcag gctgcgctgc cagagataat cacgtaaaac atcatcgcct atggggaccg
     6661 gcagccagcg gctgacatcc tcaatgttga ggtctgccag ggtggattga atgccggcgc
     6721 ccatgcccag cgggaaggtg cgcaaggcgg tttgaccgcc tacgccagcg gcaatggtgg
     6781 agtaggtttg cccaagatca acgccgagga cgcctttctg ggggtcataa attttgctca
     6841 gatagccgat catgcgtcca aacgcttgag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn ngagcaccag atcggggcgg gcctggagaa tggcatccac ctgcacttcg
     7021 ggacggcggc ggtcattgat gccaatctgt tccaccacct gaccataggt catttccacc
     7081 agccggttgg cgctttccag cgagacatct tccagcaagc caacggtgac cacgcgcagt
     7141 tcgggcccag ccgagaagac aattgtcagc cggtccaggc cagagccatc gggctgggca
     7201 ggcataatca ggcggccttc agggtccacc agaacgcgcc cggtcacttc ctgaagctgg
     7261 taaagcgcct gattgatgcc ttcaccgatg tcggagtagg gtgcgccggc tgtgctgggc
     7321 gcaaccccgg aggcgataaa gcgatattgc ccatcgacgg tatcaaacag cagtacccgg
     7381 gtcagcacag accccaggtc taccgccagt accgattcag catccaccag agaagttgtc
     7441 atagcagcca gttatcaaaa gagattcaag atggtttgga tcaggaagtt gaggcgctcc
     7501 agcagagctg tcagcgcaga agccatgacc ccggcaaaga gcgctccgag ggtgaggcca
     7561 ataaagactt cgccaatttt ggcgccggtc tcaatcaggg gcgagcggcg gggggcgccg
     7621 tcggggcggg ggcgggcgct aaaatggaag tagagcaggg tggcggccgc gccaatgacc
     7681 atcagaacgc cctgcaatag ctgaccgaag gggttggggc catccatgcc aaagggggca
     7741 attgcggcac caatctgttg gaaaagcgtg ccaaagattg cgccgcccac ggctgcgcca
     7801 gcgccagcgc ctaccagata ggccatggac acattgccca ggctggacag ccggggagaa
     7861 agcttggtca gcagcagcag gcccagaatg atgggcacgc ctgccagcac ctgacgagtc
     7921 aggtcgccgc tgaggagcgg cgcaccaagg cgggggatga tgacctcaaa aatcagcacc
     7981 acggcggtgt atcccgcgcc gacaccgacc agcaggtagg atgccaggcg gaagacgggg
     8041 ttatcgccaa acaggtagct gaagaccatt aaggtcagga gaaagccgat caatgtccag
     8101 agaaggtcga gatttcccat gtggctatgc ctctctgcgg ggcttgcgag tcttgcggcg
     8161 gcccaacatg cgggagattg cataatacaa tccgccgacc aggatgaaaa caacaatcaa
     8221 cagcatgccg atctggtagg aatcccagag gctgcgggcg gtgctgccgg ggcgctgggc
     8281 taactgatcg taagccgcgc tgccgtataa accgccgacc aatccatcaa cctgattgga
     8341 ctgcacatag ggcaaaatca ggggggcggc ctgggcgctg gaaatgacca gcaagggcac
     8401 atcacccagg gagggctgca cctgctcaat ccaggagcga gccacatcgg ggctgtcggt
     8461 gagcagcagg agcgcatcaa agtcagagag gctggctgcg ccttccagca ccggcagcga
     8521 ccaggcggat tgcccatcgg tcgtgtaggg caaagcctgc tggggcgagt tggcaaactg
     8581 cgccaggccc gattcaccgc cggggagata ccccagggag atggtttgat caaccagcgg
     8641 ataagcgggg atgtctcgca gagcggtgcg cagaagctgg tctgccagag cggtgccggt
     8701 gggcgaggtg gacagggtga tgatccgggc gttcatctgc atcaggcttt ccacagcgcc
     8761 aacactgatc aactgcattt caccggccag gcctggttcg taatccaccg ccaggagtac
     8821 ccgcggcgat tcgcctgcgg cagtcaactg ctgcatttgg gcattgaagg cgctgacttc
     8881 cggcgatgcc ccgggcaggg cagtcaactg caagccgccc aggagcggag ccagcagaat
     8941 aaccagcatg atcgcggcaa tcaaaatgcg cagaatggcc cgcggaacca gtccagtttc
     9001 gggctgggcg ccgcgcgggc gagatttttc ttcgataaac gattccagca gttcggcgtg
     9061 ggcgcgctgt cgttcggtga cccgcaggcg ggatgaatag gttggtggtt tgagatagcg
     9121 cgacgccaat tcttcactgg tcagaacgcc ctggatgccc gccaggggac cggcactttc
     9181 aatccgatca tccaccgtgc ctg
//
LOCUS       DAOPVS010000078         9194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7556_length_9194_cov_38.163913, whole genome shotgun sequence.
ACCESSION   DAOPVS010000078 DAOPVS010000000
VERSION     DAOPVS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7556_length_9194_cov_38.163913"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2014)
                     /locus_tag="PKL11_07345"
     CDS             complement(<1..2014)
                     /locus_tag="PKL11_07345"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNZ13457.1"
                     /translation="MKLEKLLGLLPENFSVIDREMIARAYHYAEDAHTGQKRAAGEAY
                     SSHCLATAVILAELRVSPTMIAAGLLHDVPLDTKSTSADIRREFGDEIGLLVDGVSRL
                     TTIPNITRADQHLEQIEAVNVSLLQPAKTDLPTDGRRTEQNNEAARKLFLSMGDDVRI
                     VVVKLAEHLHNMRTLGALPDALRLRIAQQTLDVFAPLANRLGIWQIKWELEDLAFRHI
                     NPDKFKEISDKLADRREDRELQVRAIIEQLKKIMGDASIEADISGRPKHIYSIYRKMV
                     DKGKPFEMVRDLRGVRLIVNDVPTCYMALGIIHTRWRPIPNEFDDYIAVPKENNYQSL
                     HTAVIYDDGKPLEVQIRSREMHENAEYGIASHWRYKEKGLRTDQSYEQRVNWLRRLLE
                     WRSELEDSQDALDGMKGDVFQNRVYVFTPRGDIIDLPANATPIDFAYHVHTEIGHRCR
                     GARINGKLVGLDYCLKTGDQVEILTAKQGGPSRDWLNSALGLVRTSRARAKIRAWFVH
                     QDREHNLAQGKAMLERELKRLALANVDLEKLADTFDYPSSEELYVALGSGSLPIGRVI
                     HELSEEDQEQRDQIAMGSTGTLPSSAGLSVTGLKGLLTTIARCCNPAPGDEVIGYITQ
                     GRGATIHRVDCPNLPRLTEISPDRLIKVSWGETPRTYPVDIEIKAYD"
     gene            complement(2130..2786)
                     /locus_tag="PKL11_07350"
     CDS             complement(2130..2786)
                     /locus_tag="PKL11_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ13458.1"
                     /translation="MSHLRGLVFDFDGLILETELPIFQSWQRVYEEYHAQLELSTWVR
                     CVGSSYEAFNPAIYLEEQTGLSIDRDALHQRAEIESNQIIDNQPPLPGVQRLLDEAKS
                     NGLKLAVASSSDSAWVRRHLKRLNLECYFDAICTKDDVADVKPNPELYQLALERLGLQ
                     PEEAIAFEDSPNGIRAARTAGLRCIAVPNAVTQSLDFSLASAVISSLAEVSLQKLNAF
                     "
     gene            complement(2881..4050)
                     /locus_tag="PKL11_07355"
     CDS             complement(2881..4050)
                     /locus_tag="PKL11_07355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ13459.1"
                     /translation="MSEKKIPHYDVIVVGAGSIGTPTSLFLAKAGYKVLALDPFASVG
                     QGSNKKAIGGIRATHSDPAKVRLCQRSIDIFRTWKEETGDDIEWYQGGYAFVAYREQE
                     EKALKNLVEKQKRDYQLNIDWLDHDAILQVSPDLNPKGLIGGTFSPEDGSISPLLAVH
                     SFYTQAVAAGVTFHFDEKVTELVIEQGRVVGVRTPLASYSADVVINAAGPWAKEVGAM
                     ASVNVPVNPDSHEAAITEPVAHFLEPMIVDIRPAPGSSNFYFYQHLTGQIIFCITPSP
                     QIWGMDQRETSDFLPMVTRRMLEVMPRLKNIRVRRTWRGLYPMTPDGYPIIGWTKEAE
                     GFLLAAGMCGQGLMLGPGVAELVVRMVQNKMTPEDEHILSFVSPYRDFGGQEMLK"
     gene            complement(4043..6142)
                     /locus_tag="PKL11_07360"
     CDS             complement(4043..6142)
                     /locus_tag="PKL11_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ13460.1"
                     /translation="MTDHRIKQHPILPIPEREEITFFWQGQPLQALAGDTIAAALFAN
                     GIRVFGHHAHDGAPQGIFCANGQCAQCMVIADGKPIKSCMELVTANMRVEPADGLPTL
                     PKVDDAPGMHTPQVIEVPVLIIGGGPAGLAAGIELGKLGIKVLLVDDKHRLGGKLVLQ
                     THRFFGSANAVYAGTRGIDIATKLEKDLRQYPSVEIWLQSTGLAVFSDQKVGILKEGH
                     EYVLVKPQVLLVACGAREKFLAFKGNTLPGVYGAGAFQTLVNRDLVRPAEKLFIVGGG
                     NVGLIAGYHALQAGISVVGLVEALPECGGYKVHKDKLVRMGVPIYTSHTILSANGHDK
                     VESVTIAKVDEKFRAIPGTEKSYECDSILIAVGLDPVNEFYLKAKDFGMTALVAGDAE
                     EIAEASAAIFSGKIKGMEVAKALGHDVGEIPTSWYRTGEILKSRPGKTQVEDLPEVSR
                     GVVPVMHCVQEIPCNPCTALCPHGLIYINKQDIRQIPTFLGNSDCCEVCERCVAGCPG
                     LAITLVDYRGSNADHPIVSIPYEFTKDVIKAKDKVTVLDIEGAPLGEVEVVSIHTIPT
                     SDRTLVVQVEAPKEIARQIAGIRIQEAEITKPMDEYIEHIEDDIIVCRCERVTAGDIR
                     ALIRSGYRDINEIKTVTRAGMGACGSKTCPAIIHRLFREEGIPVDEIVEATKRPIFVE
                     VPLSVFAGAEDSETSHE"
     gene            6347..7315
                     /gene="pfkA"
                     /locus_tag="PKL11_07365"
     CDS             6347..7315
                     /gene="pfkA"
                     /locus_tag="PKL11_07365"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ13461.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRAGIGQGWEVFGVRLGYEGL
                     INEKIIPLGARDVGGILQRGGTILGSARSLEFKTEAGQRKALRSLNQFGIEALVVIGG
                     NGSQTGAYALSKLGFPVVGVASTIDNDLAGSDMTIGVDTALNIALEAIDRLKITASSH
                     QRAFLIEVMGRDCGYLALAAGIAGGAEYVVIPEVETDPEEIAASMAKAYEHGKAHAII
                     VVAEGAKYNAVKLDEYFRAHKERLGFDLRVSILGHIQRGGSPSAFDRILASRLGVGAV
                     ESLARGQHGVLIGQIKSAIVATPYPEIAGVKKPLDMRLFELAKILD"
     gene            complement(7312..8404)
                     /gene="prfB"
                     /locus_tag="PKL11_07370"
     CDS             complement(join(7312..8337,8339..8404))
                     /gene="prfB"
                     /locus_tag="PKL11_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNZ13462.1"
                     /translation="MQDLIDRLQQCNDRIQAIWGRLDLPEKEAQLAQSQKQSEDNDLW
                     NDPDQARQVMKRVADLSSEIEGWTKLRKRINDALELAEMGDESLRDDLTRETEHLEKE
                     MDQRELQVMLSRPYDRGNAFITITSGAGGTDSQDWAEMLERMYLRWAERSHFGAEIID
                     RAEGEEAGIKSVTIAVTGNYAYGYLRAEKGVHRLVRLSPFDSAHRRHTSFAQVEVLPE
                     VAEDDNSIIINPDELRIDIFRSSGAGGQNVQKNATAVRLLHIPTGIIVTCQNERSQMQ
                     NRENAMRVLKARLLAIAQDQKEKELAELRGEFKKIEWGSQIRSYVLHPYQMVKDHRTE
                     YEVGNTQAVLDGELDGFIEAFLRSPELNS"
     gene            complement(8419..>9194)
                     /gene="ftsY"
                     /locus_tag="PKL11_07375"
     CDS             complement(8419..>9194)
                     /gene="ftsY"
                     /locus_tag="PKL11_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNZ13463.1"
                     /translation="LGIETTEAIIAALKKQVSQNGLTHTDELQTYLKAELLARLDAPT
                     MPDLGQYQPAVILIVGVNGSGKTTTVAKLAKQFNRQGKKVLLAAADTYRAAAVDQLQV
                     WGDRLDIPVIAGAPESDPGAVAFNAIQAAQARKSDLVFVDTAGRLHTRYNLMEELKKV
                     DRVIRKALPGAPHACWLVLDATTGQNALQQARAFKAAVQVNGVILAKLDSSARGGMTF
                     AIQRELGLPILFAGLGEKPEDLQPFDREAFIDGVLSPRQ"
BASE COUNT         2028 a         2644 c         2249 g         2273 t
ORIGIN      
        1 ggtcataggc ctttatctca atgtctaccg gatatgttcg cggcgtttcg ccccaactta
       61 ctttgatcaa acgatccgga ctgatttcag ttaaccgagg caggttgggg cagtccacgc
      121 ggtgaatcgt ggcgccgcgc ccttgcgtaa tatatccaat cacttcatcg ccaggcgccg
      181 ggttgcagca gcgcgcaatc gtggtcagca gtccttttag cccggttaca cttaaccccg
      241 ccgaactcgg cagggtgccc gtgctcccca ttgcaatctg atcgcgttgc tcctgatctt
      301 cttcactcag ctcatggata acgcgtccaa tcggcaaact tccagaccca agcgcgacat
      361 atagttcctc actgctcgga tagtcaaacg tatcggctaa tttttccagg tcaacgttgg
      421 ctaacgccag ccgcttaagt tcgcgttcca gcattgcctt gccctgagcc agattgtgct
      481 cgcggtcttg atgcacaaac caggcgcgga tttttgcgcg cgctcgtgag gttcgcacca
      541 gtcccagcgc cgaattaagc caatccctgc ttggcccgcc ctgcttggca gttaaaattt
      601 ccacctggtc gcctgttttc agacagtagt ccagcccgac cagctttcca ttgatgcgcg
      661 cacctcggca ccggtgccca atttcagtat gcacatggta ggcaaaatca atcggcgttg
      721 cattggctgg taaatcaata atatcgccgc gcggagtaaa aacataaacc cggttctgaa
      781 aaacatcgcc cttcatccca tccagcgcgt cctgcgagtc ctccagttcg ctgcgccatt
      841 ccagcagtct gcgcagccag tttacccgct gttcgtagga ttggtcggtg cgtaatcctt
      901 tttccttgta gcgccaatgt gatgcaatgc catactccgc attctcatgc atctctcgtg
      961 atcgaatttg cacttccagc ggtttcccat catcatagat aaccgccgtg tgtagcgatt
     1021 gatagttgtt ctctttgggc actgcaatgt aatcatcaaa ttcatttgga attgggcgcc
     1081 accgggtgtg gataatgcca agcgccatgt agcacgtagg cacatcattg acaatcaatc
     1141 gcacgccgcg taaatcgcgc accatttcaa atggcttgcc tttatccacc atttttcgat
     1201 aaatcgaata aatgtgtttg ggccgtccag agatatccgc ctcgatactg gcatcaccca
     1261 taatcttttt caactgctca atgatcgccc gcacctgcag ctcgcggtct tcgcggcggt
     1321 ctgctagctt atccgaaatc tctttgaact tgtccgggtt gatgtgccgg aaagccaggt
     1381 cttccagttc ccatttgatt tgccagatgc ccaaccgatt cgccagcggc gcaaatacat
     1441 ccagcgtttg ctgggctatt cgcagccgca gcgcatcggg gagtgcgccc agagtccgca
     1501 tattgtgcaa atgctctgca agttttacaa ccacaatccg aacatcgtcc cccatggata
     1561 agaagagttt gcgcgcggcc tcattatttt gttcagttcg tcgtccatct gttggcaggt
     1621 ccgttttggc gggctgcagc aaactgacgt taacggcctc aatttgctcc aggtgctggt
     1681 ctgcccgggt gatgttggga atggttgtta accggctgac gccatcaacc aatagcccaa
     1741 tctcatcgcc aaactcgcgt cgaatatcgg ccgaagtgga tttagtgtct aggggaacat
     1801 cgtgcaataa ccccgccgca atcatggtcg ggctgacgcg taactcagcc agaatgaccg
     1861 ccgttgccag acaatgtgaa ctgtaggcct cgcctgctgc ccgcttttgg ccggtatgag
     1921 catcctcggc atagtgatag gcccgagcaa tcatctcgcg gtcaatcaca ctgaaatttt
     1981 caggcagcag accaagcagt ttttcgagtt tcaaacgcgt cctcgtttgg aaaatattaa
     2041 tcgcccaagg ccctgatctg tgaccaccaa gaccaaacaa tcatctggac aagtatacac
     2101 ttggagcatc ggttaattca aggggccgat taaaacgcat tgagcttttg taggctcact
     2161 tcagccagtg atgagatgac cgcactggcc aggctgaagt caaggctttg ggtgacagca
     2221 ttgggaaccg caatgcagcg caaaccagcg gtgcgtgctg ccctgatgcc gttcggcgaa
     2281 tcctcaaagg caatcgcttc ttcgggttgc agtcctaatc gctccagggc taattgatac
     2341 aactccgggt tgggtttcac atctgccacg tcgtctttgg tgcaaatggc atcaaaataa
     2401 cactcgagat tcaaccgctt caagtggcgg cgcacccagg cggaatctga actggaagcc
     2461 actgccagtt tgagcccatt agatttcgct tcatccagca gccgttgaac tcccggtagg
     2521 ggcggctggt tgtcaataat ctgattggat tcgatctcag ctcgctgatg cagcgcatcc
     2581 cggtcaattg acaatccggt ttgttcttcc agatagatag ccggattgaa ggcctcgtaa
     2641 gacgacccca cgcagcgcac ccaggtgctc agctctaatt gggcatgata ttcttcatag
     2701 acgcgctgcc agctttgaaa aatgggcagc tcggtctcaa gaatcagacc atcaaaatca
     2761 aaaaccaatc cacgcagatg actcattgac ctcatccatc cggtgcaaaa attaccacaa
     2821 ggcgcaccgg gcatttatta tacccggtgc gccgcataat tcacggatat ttcgaggtgt
     2881 ttacttgagc atttcctgcc cgccaaaatc gcggtacggc gacacaaagc tcaaaatatg
     2941 ctcatcctca ggtgtcatct tattctgaac cattctgaca accagctcag ccacgcccgg
     3001 cccaagcatc aaaccctgtc cgcacatccc cgccgccagc aagaagccct cggcttcttt
     3061 ggtccacccg atgatcgggt aaccgtctgg tgtcatgggg tacaacccgc gccatgtacg
     3121 ccgcacacgg atattcttca gccgcggcat cacttccagc atacgccggg tcaccatagg
     3181 cagaaagtca ctggtttcgc gttgatccat cccccagatc tgtgggctgg gggtaatgca
     3241 gaagataatc tgtccagtca ggtgctggta gaaataaaag ttagaagaac ctggcgcagg
     3301 tcggatatcc acaatcatcg gttcaaggaa atgcgccact ggctcggtaa tagccgcctc
     3361 gtgcgaatca gggttcaccg gtacattcac acttgccatc gccccaactt cttttgccca
     3421 gggcccggcc gcgttgatca ccacatcggc gctgtagctt gccagaggtg ttcgtacgcc
     3481 aaccacccga ccctgctcaa tgaccagctc tgtcaccttt tcatcaaagt ggaaggtgac
     3541 gcccgcggcc acggcctggg tgtagaatga atgcactgcc agcaatggcg agatgctccc
     3601 gtcttccggc gagaaagtgc cgccaatcaa cccttttgga ttgaggtctg gcgatacttg
     3661 cagtatggcg tcgtgatcca gccaatcgat attaagctga tagtctcgct tctgtttctc
     3721 aaccaggttt ttaagtgcct tctcttcctg ttctcggtaa gccacaaaag catatccacc
     3781 ctggtaccac tcaatatcat cgccggtttc ttccttccat gtgcgaaaaa tgtcaatact
     3841 tcgctggcac agacgcactt ttgccgggtc tgagtgggtg gcgcgaatac cgccaattgc
     3901 ttttttgttt gacccctgtc caacactggc aaaggggtct aatgctaaaa ctttatatcc
     3961 ggcctttgcc aaaaacaaag aggttggggt gccaatcgaa ccagcaccaa caacaatgac
     4021 atcatagtgc gggattttct tctcactcat ggctggtctc cgaatcttct gcgccggcaa
     4081 agacactgag cggcacttct acaaaaatgg ggcgttttgt tgcttccaca atttcatcta
     4141 ccggaatgcc ttcctcgcgg aataaccggt gaataatggc cgggcaggtt ttagacccac
     4201 aggcgcccat gccagccctt gttacggtct tgatttcgtt gatatcgcgg tatccagatc
     4261 gaattaacgc ccggatatct ccggcagtaa cgcgttcaca gcggcataca atgatgtcat
     4321 cctcaatatg ctcgatatac tcatccattg gcttggtgat ctcggcctct tgaatgcgaa
     4381 tacctgcaat ctggcgtgca atttctttgg gcgcctccac ctgaacaacc agggtccggt
     4441 cacttgtcgg gatcgtatga atggaaacca cttccacttc gccaaggggg gcgccttcaa
     4501 tatccagcac ggtcaccttg tctttggctt tgatgacatc tttcgtaaat tcgtagggga
     4561 tcgagacaat ggggtggtct gcgttgcttc cccggtagtc caccagcgtg atcgccagcc
     4621 ctgggcagcc tgccacgcaa cgctcgcaca cttcgcagca gtcactgttc cccaaaaagg
     4681 tcggaatttg tcgaatatct tgcttattga tgtaaatcaa cccatgcggg caaagtgcgg
     4741 tgcaggggtt gcagggtatt tcctgcacgc agtgcatcac cggaaccacg ccgcggctga
     4801 cttccggcag gtcctccacc tgtgttttcc caggtcgcga cttcaagatc tcgccggtcc
     4861 gataccacga ggtcggaatt tcacccacat cgtgacccag cgccttggct acttccatcc
     4921 ccttgatttt gcccgagaag atcgccgcag aggcttcggc aatttcttcg gcatccccgg
     4981 ccaccagcgc agtcatccca aaatccttgg ctttgaggta aaactcattg accgggtcga
     5041 gcccaaccgc gattaaaatg ctgtcacatt cgtaagattt ttcggtgccc ggtatggctc
     5101 taaacttctc gtccactttg gcaatcgtca ccgattcaac tttatcgtgt ccatttgcgc
     5161 tcaagatggt gtgagaagtg taaattggca cgcccatccg taccagttta tctttatgta
     5221 ctttgtagcc gccgcactcg ggcagggctt ctaccagacc aaccacgctg atcccggctt
     5281 gaagcgcatg atacccggca atcaacccca cattgccgcc gccgacgatg aacagttttt
     5341 cagcagggcg caccagatcg cggttcacca gcgtctggaa cgcaccagcg ccataaacac
     5401 ccggcagggt gtttcccttg aacgccagga atttttctcg ggcgccgcag gccaccagta
     5461 agacttgcgg tttcaccagc acatactcgt gaccttcctt caaaatcccc actttctgat
     5521 cactgaacac cgccagcccc gtgctttgca gccagatttc tacgctgggg tactgccgta
     5581 aatctttttc cagtttcgtt gcaatatcaa tcccacgcgt gcccgcatag acggcatttg
     5641 ctgagccaaa aaagcggtgt gtttgcagca ccagtttacc acccaaacga tgtttgtcgt
     5701 ccaccagcag tactttgatg cccagtttac ccagttcgat accggcagcc agacctgccg
     5761 gtccgccacc gataatcagt actggtactt caataacctg tggcgtgtgc atgccggggg
     5821 catcgtcaac tttgggcagt gttggcaggc catcggctgg ctcaacccgc atattggccg
     5881 tcaccagttc catgcaagac ttgatgggtt tgccatctgc aataaccatg cactgtgcac
     5941 attgtccgtt ggcgcaaaaa atgccctgcg gcgcgccgtc gtgggcatgg tgaccaaaca
     6001 cacgtattcc attggcaaag agggccgccg caatcgtatc accagccagt gcttggagcg
     6061 gttgtccctg ccagaaaaag gtaatctctt ctctttcagg aatcggcaaa atagggtgct
     6121 gcttaatccg atgatcagtc atattttttc tttaaacctc tatggagaac agcgttgaga
     6181 caattaccgg ccagagcacg gtaaaaaact attgaaattg tagcattgac gttttgtact
     6241 gaatactgct atttagccat agttaatatc acgagtgtca caaaagctaa atcatgttaa
     6301 aatagagtga ttaattttta cctgattccc tcattggagg ttttgtatga aacgaattgc
     6361 agttctcact agcggcggtg atgcgcccgg catgaatgcc gctattcgcg cggttgttcg
     6421 cgctggcatt ggccagggtt gggaagtctt tggcgttcgc ctgggttacg aaggtttgat
     6481 caacgaaaaa atcattcctc ttggcgcccg agatgttggt ggcattcttc agcgcggcgg
     6541 caccattttg ggcagcgccc gctccctgga attcaagacc gaggctggcc agcgcaaggc
     6601 tttacgctcc ctcaatcaat ttggaattga agccctggtt gtcatcggcg gcaatggatc
     6661 gcaaactggc gcctatgcct tatccaagct cggcttcccg gttgttggcg ttgcctcaac
     6721 tatcgacaac gatctggcgg gctcagatat gaccatcggc gtggataccg ccctgaatat
     6781 cgctctggag gccattgacc gcctgaaaat cacagcctca tcccaccagc gcgccttcct
     6841 gatcgaagta atgggccgcg actgcggtta cctggcgctg gctgccggca ttgctggcgg
     6901 cgctgaatat gtggtgatcc ccgaagttga gacggatccc gaagaaattg ccgccagcat
     6961 ggccaaagca tacgagcacg gcaaagccca tgccatcatc gtggtagccg aaggggctaa
     7021 atataacgcc gttaaattag acgaatactt ccgcgcccac aaagaacgcc tcggctttga
     7081 tctccgcgtc tcaattcttg ggcacattca gcgtggcggc agccccagcg cctttgatcg
     7141 cattctggcc agccgcctgg gcgtgggtgc cgttgaatct ctggcgcgtg gccagcacgg
     7201 tgtcttgatt ggtcagatta aaagcgccat tgtcgctacc ccatatcccg agatcgcggg
     7261 cgtcaaaaag cccctggata tgcggctgtt tgaactggca aaaatcttgg actagctgtt
     7321 caattccggc gaacgcagga aggcttcaat aaagccatcc agctcgccat ccagcactgc
     7381 ctgggtgttg ccaacctcat actcagtccg gtgatctttc accatctggt atggatgcag
     7441 cacataagat cgaatttgcg atccccactc aatcttcttg aactcgccgc gcagctcggc
     7501 gagttctttt tctttctggt cctgggcaat agccagtaga cgcgctttca acacccgcat
     7561 ggcattttcc cggttttgca tttgagatcg ctcattttga caggtgacaa taatgccagt
     7621 tggaatatgc agcagccgca ccgccgtggc atttttctgc acgttctgtc cgcctgcacc
     7681 agatgagcgg aagatatcaa tccgcagttc gtccggattg atgataattg aattatcatc
     7741 ttcagccact tcgggcagca cttccacctg cgcaaatgag gtgtgccgcc ggtgtgctga
     7801 atcaaatggc gaaaggcgca ctaatcgatg cacacccttt tcggcgcgca aatagccata
     7861 agcataattg ccggtcaccg caatggtgac gcttttaatc cccgcttcct ccccttccgc
     7921 ccggtcaata atttctgcac caaaatggct gcgttctgcc cagcgcaaat acatgcgctc
     7981 cagcatttct gcccaatctt gcgaatccgt gccgccagcc ccagaggtaa ttgtaataaa
     8041 tgcatttccc ctgtcgtacg gtcttgaaag catcacctgg agttcacgct gatccatttc
     8101 tttttccagg tgctcggttt cgcgggtcaa atcatcccgc aaactttcat cgcccatttc
     8161 tgccagttcc aacgcatcgt tgatgcgctt gcgcagcttg gtccagcctt caatttcgct
     8221 gctcaggtcg gccacgcgct tcatgacctg acgagcctgg tctgggtcat tccagagatc
     8281 attatcttcg gattgttttt gagattgtgc tagctgggct tctttttcgg gtaagtcaaa
     8341 gacgccccca gatggcctgg attctgtcgt tgcactgctg gagtcgatcg atcagatctt
     8401 gcatcacgtg ttctcccgtt attgccgtgg cgacaggacg ccatcaataa aggcttcccg
     8461 gtcaaagggt tgcaggtctt cgggtttttc acccagtcct gcaaaaagaa ttggcagccc
     8521 cagttcgcgt tgaatggcaa aggtcatccc accgcgggct gaagaatcaa gcttggccag
     8581 gatcacgccg ttcacctgca cggcggcttt gaacgcccgg gcttgctgca gtgcattctg
     8641 acctgtggtc gcgtccagca ccagccagca ggcgtgcggt gcccctggca gcgcctttcg
     8701 aattacccga tcaacctttt ttagttcttc catgaggttg taacgagtat gcagtcgccc
     8761 ggcagtatca acaaacacaa ggtcagattt gcgagcctgg gcagcttgaa tggcgttaaa
     8821 cgccaccgca cccgggtcgc tctccggcgc gcctgcaatt actggaatat ctaatcgatc
     8881 cccccacacc tgaagctgat caactgccgc agcccggtag gtgtcggcag cagccagtag
     8941 aacttttttc ccctgtcggt tgaactgttt ggcaagttta gcgacggtgg ttgtcttgcc
     9001 agatccattt acacccacaa tcaaaatcac cgcgggttga tattgcccca gatcaggcat
     9061 cgttggggca tctaaccgcg ccagcagttc tgccttcaag taagtttgca gctcgtcggt
     9121 gtgggtgagc ccattctgac taacctgctt cttgagagcc gcaataattg cctcggttgt
     9181 ctcaatcccc aggt
//
LOCUS       DAOPVS010000079         9078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7666_length_9078_cov_58.979275, whole genome shotgun sequence.
ACCESSION   DAOPVS010000079 DAOPVS010000000
VERSION     DAOPVS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7666_length_9078_cov_58.979275"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PKL11_07380"
     CDS             <1..973
                     /locus_tag="PKL11_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ13464.1"
                     /translation="KMLPNAIPGILLFIDTEGRILGANEEFAQGFDRKLSDVIGARLA
                     DILPEPLMRSRWAHIQQTIESGIPCQFSDERNGKHFSNYLYPVRDPSGAVSRVGIFVT
                     DITRQKDTESQLAEIHAQYEADQKASQTEQAGFESRRLRAMQRLRGKLLARLESLRQT
                     ELSSNQMDIILEMETFLEQAGFLVGSERYSRTERPAKKIQILLVEDNQIIADNFRSYM
                     EAKGSQVLVALDGQAALDIAEQTWPDIVFMDISLPDMDGLEVVQKLRGMPEFTSTPII
                     VLTAHGNSERERCLRAGASDFLTKPVSLKNLMETAERWLRNPQGGTA"
     gene            complement(1013..1087)
                     /locus_tag="PKL11_07385"
     tRNA            complement(1013..1087)
                     /locus_tag="PKL11_07385"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Met,seq:cat)
     gene            1221..1601
                     /locus_tag="PKL11_07390"
     CDS             1221..1601
                     /locus_tag="PKL11_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13465.1"
                     /translation="MKIAKILALLVAGGLILFGAIYIMAAFSPSAQGTPGGLVITGLL
                     LAGMGFGIGFAAIKFMKPPAPVVTQQTTIKIDLPGNVSMDTIKCKSCGGTLSTDNIAM
                     VAGAPVVTCPYCHTSYQLTEEPKW"
     gene            1634..3304
                     /locus_tag="PKL11_07395"
     CDS             1634..3304
                     /locus_tag="PKL11_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13466.1"
                     /translation="MRRFRLLSLLIAIVLLIGLGGTASAQTYLFEVERAEAAIYIESD
                     GSATVEYTYTFVNDPSADPMEYVDIGMPNTYYYLSNITAEINGERISDITDSPYISNG
                     VALGLGRNAIKPGARGVVHVRITNIDKMLYTTKIDGQSYASFNFMPNYFDSSLLVGST
                     DMTVTLYLPVGVESEQPRWYTPRGWPGESEPLSGYDEAGRVFYQWNSPEASASVGYKF
                     GAAFPVNLVPASTVKKPSIWETLGITEEDFMGGAFFCCCSIMFIGFPIWGAINARKRR
                     LQYMPPKISVEGHGIKRGLTAVEAAILMERPLDKVMTMILFGLLKKSAARVVTREPLT
                     LESADPLPEGLYAYETDFLAAFKKTDKAEQTKALTEMVVNLVKSLTEKMKGFSRKETI
                     DYYESIMKTAWEQVEAANTPEVKSERYEQALEWTMLDRDYDDRTRDVFRTGPVFYPTW
                     WWRFDPGHASTAGGGGLAQPVGPSNRSAVPGADFAASVVNGVQNFSSNVVRDLNSFTS
                     GITNKTNPVPVSSTSRGGSYRSGGGGGRSCACACACAGCACACAGGGR"
     gene            3312..4676
                     /locus_tag="PKL11_07400"
     CDS             3312..4676
                     /locus_tag="PKL11_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ13467.1"
                     /translation="MIDWWGQLTRWWQKNRRINPPGAGVYHFERAEDGGQTRIHLRLE
                     VDGRGLLLVNANHAYHLNPSAALMAYLALRKVSSAQAAGTLTRAYNLPRAQAAADYAA
                     FDEQLEALIQPNGPCPICELELDTMMPFSAQLSAPYRMDLALTYRCNNDCAHCYNARP
                     RNHPELSTEQWKTILDKLWALGIPHIVFTGGEPTLRPDLPELIAHAEHNGQITGLNTN
                     GRRLKDPHLLQRLLDAGLDHVQITVESHDPAVHDAIVCSPGAWEDTIAGVKNALASRL
                     YVMTNTTMLQSNSPYLRETLAFLAELGVPTVGLNALIYSGKGAQVGTGLSAEALPALL
                     TLARQMTDDHDQRLIWYTPTQYCHFDPIQMDLGVKGCTAALYNMCVEPDGQVLPCQSY
                     YTSLGSLLTDPWESIWNHDLAVRLRTRRDLPEACGHCDLLTECGGGCPLEWAVQSPTE
                     VQGL"
     gene            4673..6175
                     /locus_tag="PKL11_07405"
     CDS             4673..6175
                     /locus_tag="PKL11_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13468.1"
                     /translation="MMQVLQAVGQIFLRTLYYFYLVPFREAFLNLKELPKPQRTLAIV
                     GYSVTGALLIFLLVVELWGGGMGQVTFTRSDGSEMRQIPLAVIIVSMAAMALGWAFLL
                     TGASDGHPVVFIPIAIIFGIQLMCVIPFENTPALIAWCCSAPTLILAAAGAHIFTHKK
                     PVWKELQVGEFIIWGAAGVFFLGLWGFTRGGLDLMAQDLDGIYSLVMLSFIPIWMLAG
                     MAVVDAAVDLGRRFIKLIREKLSDEALLALTAFLIITRPVITVALFIIDQINGTQITL
                     QENPLKTALVLDGIFSVPLLIALVIVRLSKHWNARSAAVLLGTSVATPAFTLGLAMAL
                     LGQNIFDPFEMTASSLGLMPSLLVFVALVVHTTLSMGTRFAEKGGKFLPRSGRIMFIF
                     GMVLGLLSFTLFFVNTRSAETGLLDRQIQDGINIFFSLSVFLFGGPYLLWILIRHRER
                     LAGPAEDYAPRPEAVEGQTAPRKQQWLTIATVSAVILLSLACGLALLFNQ"
     gene            6204..7361
                     /locus_tag="PKL11_07410"
     CDS             6204..7361
                     /locus_tag="PKL11_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ13469.1"
                     /translation="MKKILEYWVDRLFGRVKPLAAGVYTYQSPPEVQPPSRMHLRVEP
                     DGQSILILNASTVLHLNQTATEYAYHLVKGTERAEMAGKMAGRYRVSREQALYDYEAL
                     SAQIQSIIETPDLDPETFLGMERMEPYGGRITAPYRLDCALTYRVSDNSSTAPQERVK
                     RDLLTEEWQSILQKAWQAGIPHIVFTGGEPTLRPDLPELIAYAEKQGQVTGLLTDGLR
                     LAETDYLHSLLNAGLDHLMIVLKDDEQCWEAIRDVLAEDIYTTVHITLTPANAASLTE
                     TLDELAKLEVSSISLSASEASLNAALKDAAQAAAERGFKLVWDLPTPYSSLNPVAVEL
                     AEAGERSEGAGRAWLYVEPDGDVLPGQGILTVMGNLLTDSWETIWKNARPE"
     gene            7358..8107
                     /locus_tag="PKL11_07415"
     CDS             7358..8107
                     /locus_tag="PKL11_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNZ13470.1"
                     /translation="MTPPPPTRRCQQQARWTAQLRQYLYGLAGLESARRVLEVGCGSG
                     AVLADFAGWNGQVLGVDLDRPALVESQGQAGFARLQQADAGALPYAAHCFDLSLCHFL
                     LLWVANPVATLQEMRRVTRAGGAVLALAEPDYGGRVDYPPPLDEMGRRQAQGLARQGA
                     DPLAGRKLSGWLHEAGLVDIQVGVLGGWWAEPPEEAEQALEWQTLQHDLDGLASASEL
                     ENWRKINATAWKTGQRVLYVPTFYGWGRVPG"
     gene            8131..8940
                     /locus_tag="PKL11_07420"
     CDS             8131..8940
                     /locus_tag="PKL11_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNZ13471.1"
                     /translation="MDHYRVKPDQKIELDAWDASETSQFKGSKDDALPRFEKLAAELG
                     ELQEVMFAEGKHKVLIVLQAMDTGGKDGTVRAVFGRVNPQGVRVANFKAPTATELAHD
                     YLWRVHQQVPGRGELVIFNRSHYEDVLVVRVHGLVPEEVWKKRYEQINAFEKMLVEEG
                     TTIVKFYLHIDKDEQRERLLERIDVPEKRWKFNPGDLEERKLWKDYMKAFEAVLERTS
                     TPWAPWYIVPANRNWYRNLVVADVLVETLRALKMKYPQPQEDLTQYRGQLG"
     gene            9000..9074
                     /locus_tag="PKL11_07425"
     tRNA            9000..9074
                     /locus_tag="PKL11_07425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9035..9037,aa:Pro,seq:ggg)
BASE COUNT         1992 a         2211 c         2920 g         1955 t
ORIGIN      
        1 caagatgctg cccaatgcga ttccgggaat tttgctgttt attgataccg agggccggat
       61 tttgggcgcc aacgaagaat ttgcacaggg atttgaccga aagttgagtg atgtgattgg
      121 cgcccggttg gcggatattc tgccggagcc gctcatgcgg agccgatggg cccatattca
      181 gcagacgatt gagagcggga taccctgcca attcagcgat gaacgcaacg ggaagcattt
      241 ttccaactac ctgtacccgg tgcgagatcc gtctggagcg gtgagccggg tggggatctt
      301 tgttacggat attacgcggc aaaaagacac tgaatcccaa ttagccgaga ttcacgccca
      361 gtatgaagct gatcagaaag caagtcagac cgagcaggca ggctttgaat ccaggcgatt
      421 gagggcgatg cagcgattga gaggcaagct gcttgcccgg ttggagagcc tgcgccagac
      481 cgagctgtct tcaaatcaaa tggatattat cctggagatg gaaaccttcc tggaacaggc
      541 aggttttctg gtgggaagtg aaaggtacag ccggacagaa cgcccggcaa agaaaattca
      601 gatcttgctg gttgaagata atcagattat tgcggataac tttcgcagct acatggaagc
      661 caagggcagt caggtgctgg tggcgctgga tggacaagcg gcgctggaca ttgcagaaca
      721 aacctggccc gatattgtgt ttatggatat cagcctgccc gatatggacg ggctggaggt
      781 tgttcaaaag ctgcggggaa tgccagaatt taccagtacg ccgattattg tgctgactgc
      841 gcacggcaat tcagaacgcg agcgctgcct gcgagctgga gcgagcgatt ttctgaccaa
      901 gccggtgagc ttgaaaaacc tgatggagac ggctgaacgc tggctgcgca acccccaggg
      961 tggaactgcg taaatctatt gatgaaaaga aaatccgcct tgcggcggat tatggtggcg
     1021 agggtgaggg ttgaactcac gaccaagggc ttatgagtcc cctgctctcc cactgagcta
     1081 cctcgccaag cgaccgcaat cttactacga tccgccggtt ttgtcaaatt atttaagatc
     1141 cggcaaaaag aagcaaatga tagttgcctg gaatggcaga taatgttaaa ctaaaggtaa
     1201 tttcccggag gtacaccatc atgaaaattg ctaagattct ggcgctgctg gtcgcggggg
     1261 gattgattct gtttggcgcg atttatatta tggcggcgtt cagccccagt gcacagggaa
     1321 cccctggcgg gttagtgatc accgggctgc tgctggcagg tatgggattt gggatcgggt
     1381 ttgcggcgat taagtttatg aagccgccgg cgcccgttgt gacccagcag acgaccatca
     1441 agattgattt gccgggcaat gtgagcatgg acacgattaa gtgcaaatct tgcggtggaa
     1501 cgctttctac cgataacatc gccatggtgg ctggcgcacc ggtggtcacc tgcccgtact
     1561 gccacacctc gtatcaattg actgaagaac ctaaatggta aagggttccc caaaccgggg
     1621 aggaggagac acgatgcgac gattccgcct gttgagcttg ctgattgcaa ttgtcctgtt
     1681 gattggttta ggaggaaccg cctcagctca gacgtatttg tttgaggttg aacgtgcgga
     1741 ggcagccatt tatattgagt cggacggcag tgcgacggtt gaatatacct acacattcgt
     1801 caacgaccca tcggctgacc caatggaata tgtggatatt gggatgccaa acacctatta
     1861 ctatctcagc aatattacgg ctgagattaa cggggaacgg atcagcgata ttaccgattc
     1921 gccgtacatt tcaaacggtg tggcactggg gctggggcga aatgcgatta aacccggagc
     1981 gcgcggcgtc gtgcatgtca ggatcacgaa tattgacaaa atgctgtaca cgaccaaaat
     2041 tgacgggcag agttatgcca gcttcaattt tatgccgaac tactttgatt ccagcctgtt
     2101 agttggctcg accgatatga ccgtcacgct gtatttgccg gttggcgtgg agtctgagca
     2161 gccccgctgg tacaccccgc gcggctggcc cggagagagt gagccgctca gtggatatga
     2221 tgaagcaggg cgggtattct atcaatggaa cagtccggaa gcaagcgcgt cagtaggata
     2281 caagtttgga gcagcattcc cggtaaacct ggtgccagcc agcacagtca agaagcccag
     2341 catctgggaa acgctgggga ttaccgagga agactttatg ggtggggcgt tcttctgctg
     2401 ttgttcaatc atgtttattg gttttccgat ctggggtgcg atcaatgccc gcaaacgacg
     2461 gctgcaatat atgcccccca agatcagtgt tgaagggcat ggcatcaagc gtgggctgac
     2521 agcggtggag gcggctattc tgatggagcg tccgctcgac aaagtgatga ccatgattct
     2581 ctttgggctt ttgaaaaaga gcgctgcccg cgtggtcacg cgtgaaccgt tgacgctgga
     2641 atctgcggac ccgctgcccg aaggcctgta tgcttatgaa accgattttt tggcagcgtt
     2701 caagaagacc gataaggctg aacaaacaaa ggctctcaca gagatggttg tcaacctggt
     2761 taagagcctg acggagaaaa tgaagggctt cagccgcaaa gagaccattg attattacga
     2821 gagcatcatg aaaaccgcct gggaacaggt ggaagctgcg aacaccccgg aagtgaagag
     2881 cgagcgctac gaacaggcgc tggaatggac aatgctggat cgtgattatg atgatcggac
     2941 acgcgatgtt tttcgcacgg gaccggtctt ttatccgacg tggtggtggc ggtttgaccc
     3001 gggtcatgca agcacggcgg gcggcggcgg gctggctcaa ccggtggggc cgagcaaccg
     3061 atcggcggtg cctggagctg attttgcggc gagtgtggtt aacggcgtgc agaatttctc
     3121 atccaatgtc gtgagagatc tgaacagctt caccagcggt attacgaata aaaccaatcc
     3181 ggtgcctgta tcaagtacgt ctcggggtgg ttcttaccga agcggcggcg gaggtgggcg
     3241 gtcgtgcgcc tgcgcctgtg catgtgcggg ttgtgcctgt gcgtgcgccg gcggcggacg
     3301 atagattgac catgattgac tggtggggtc agctcacccg gtggtggcag aaaaaccggc
     3361 ggattaatcc gccgggcgct ggcgtgtatc actttgagcg ggcggaggac ggcggtcaga
     3421 cccgcatcca cctgcggcta gaggtagatg ggcgcggcct gctgctggtt aacgcgaatc
     3481 atgcttatca tctgaatcca agtgcggcgc tgatggccta cctggcgctg cgcaaggtca
     3541 gttcggcaca ggcagccgga accttaacac gcgcgtataa cctgccgcgg gcccaggctg
     3601 cggcggacta tgcggctttt gacgaacaac tggaagcact tatacagccg aatggacctt
     3661 gcccaatttg tgagctggaa ctggatacca tgatgccgtt ctcggcgcag ctttcggcgc
     3721 cttaccgcat ggacctggca ctgacctacc ggtgcaataa tgactgcgcc cactgctata
     3781 acgcgagacc cagaaaccac ccggaattga gcactgagca atggaaaacg attctagata
     3841 aactgtgggc gctggggatt ccgcacattg tattcactgg cggtgagcct acgctgcgcc
     3901 ctgacctgcc cgagttgatt gcccatgccg agcataacgg gcagattacc gggttgaata
     3961 ccaacgggcg gcgattgaaa gatccccatt tgctgcagcg attgctggat gcggggctgg
     4021 atcatgtgca gattacggtt gagtcgcatg acccggctgt tcacgatgcg attgtttgct
     4081 cgccgggcgc ctgggaggat acgattgcgg gggtcaagaa tgcgctggcg agccggttgt
     4141 atgtgatgac taataccaca atgctgcaga gcaacagccc gtatttgcgg gagacgctgg
     4201 cctttctggc agaactgggt gtgccgacgg ttgggctgaa tgccttgatt tactccggga
     4261 aaggggcgca ggtgggaacc gggttgagcg ccgaagcgct gcccgcgcta ttgacactgg
     4321 cgcgtcagat gactgatgat catgaccagc gcctgatctg gtacacacca acccagtact
     4381 gccattttga cccgattcaa atggacctgg gagtgaaggg ctgcacggcg gcgctgtaca
     4441 atatgtgcgt ggaaccagat ggacaggtgc taccctgcca atcttattac accagcctgg
     4501 gcagtctgct gacagatcca tgggaaagca tctggaatca tgatctggcg gtgcggctgc
     4561 gaacccgccg ggacctgccc gaggcctgcg ggcactgcga tctgctgacg gaatgcggcg
     4621 gtggctgtcc gctggaatgg gcagtacagt caccgacaga ggtgcagggg ctatgatgca
     4681 ggtgctgcaa gcggttgggc agattttttt acggaccctg tattattttt acctggtgcc
     4741 gtttcgggaa gcattcctca acctgaaaga actgcccaaa ccgcagcgaa cgctggcgat
     4801 tgttggctac agcgttacgg gggccttgtt gatctttttg ctggtggttg aactgtgggg
     4861 cggcggcatg gggcaggtca ctttcacgcg ctcagacggg tcagagatgc ggcaaattcc
     4921 gctggcggtg attattgtca gtatggcggc catggcgctg ggatgggctt tcctgctcac
     4981 gggagcaagt gacgggcacc cggttgtttt catcccgatc gcgatcatat tcggcatcca
     5041 actgatgtgc gtcattccgt ttgaaaacac cccagcgttg atcgcctggt gctgctcggc
     5101 gccgacgctg attctggcgg ctgcgggagc gcatattttc acccacaaga aacccgtctg
     5161 gaaagagctg caggtgggag agtttatcat ctggggggcg gcaggagttt tcttcctggg
     5221 gttgtggggc tttacgcggg gtggattaga tttgatggcc caggatttgg atgggatcta
     5281 ctcgctggtg atgttgtcgt ttatcccgat ctggatgctg gcggggatgg cggtagtgga
     5341 tgcggcggtt gatctggggc ggcggttcat taagctcatt cgcgagaagc tgtcggatga
     5401 ggccctgctg gctttgacag cgtttctgat catcacacga ccggtgatta ccgttgcttt
     5461 gtttattatt gatcaaatca acggaacgca aatcacgctg caggaaaacc cactgaagac
     5521 ggcgctggtt ttggatggga ttttctcggt gccattgttg attgccctgg tgattgtgcg
     5581 gttaagcaag cattggaatg cacgctcggc agcggtgctg ctgggcacca gcgtggcaac
     5641 gccagccttc acgctggggt tggccatggc gctgctgggg cagaatatct ttgacccgtt
     5701 tgaaatgacc gcctccagcc tgggtttgat gccatcgctg ctggtctttg tggcgctggt
     5761 ggtgcacacc accctgagca tgggaacccg gtttgccgaa aaaggcggta agttcctgcc
     5821 gcgcagcgga cgaatcatgt tcatttttgg gatggtgctg gggctgctca gcttcactct
     5881 gttctttgtt aatacaagat ctgccgagac gggactgctg gatcggcaaa tccaggatgg
     5941 cattaatatt ttcttcagcc tcagtgtctt tctatttggc gggccgtatc tgttatggat
     6001 tttgatccgg caccgcgagc ggctggcagg cccggctgag gattatgcgc cgcggccaga
     6061 agccgtggaa ggccagacgg cgccccgcaa acagcagtgg ctgacgattg ctaccgtcag
     6121 cgcagtgatt ttgctcagcc tggcttgcgg gctggcgtta ttattcaatc aataaacgcg
     6181 atttttgttc aggaggcttg cgtatgaaaa agattttaga atactgggtt gaccgcctct
     6241 ttggccgcgt caaaccactg gcggcaggcg tttataccta ccaatcgcct ccagaggttc
     6301 aaccccccag ccggatgcac ttgagagttg aaccagatgg gcagagtatt ttgattttga
     6361 acgccagcac ggtgctgcat ctcaatcaaa cagccaccga gtatgcctat cacctggtca
     6421 aaggcaccga gcgggctgaa atggccggga aaatggcggg gcggtaccgg gtgagccggg
     6481 aacaggcgct gtatgactac gaggccttga gcgcacaaat tcaatcgatc atcgagacgc
     6541 ccgacctgga cccggagacc ttcctgggaa tggaacggat ggagccgtat ggcggtagaa
     6601 tcaccgcgcc ttaccgcctg gactgcgccc tgacctatcg ggtttctgat aacagcagca
     6661 cagcgccgca ggaacgagtc aagcgggatt tgctgactga agagtggcag agcattttgc
     6721 aaaaggcctg gcaggcgggc attccgcaca ttgttttcac cggcggcgaa ccgaccttgc
     6781 gacctgatct gcccgaattg atcgcctatg cagaaaagca gggacaggtt accgggctgc
     6841 tgacagacgg gctgcggctg gcagagacgg attatctgca cagcctgctg aatgccgggc
     6901 tggatcattt gatgattgtg ctgaaagacg atgagcagtg ttgggaagca atccgcgatg
     6961 tgctggctga ggatatttac accacagttc atatcacgct gactccggcg aacgcggcaa
     7021 gcctgaccga aacactggat gaactggcca aattggaagt gagttcaata tcactgagcg
     7081 ccagtgaggc cagcctgaat gccgccttga aagacgccgc acaggcagcc gccgagcggg
     7141 gcttcaagct agtctgggac ctgccaacgc cctactccag cctgaatccg gtggcggtgg
     7201 agctggccga agccggtgag cggtctgaag gcgctggccg agcatggctg tatgtggaac
     7261 cggatggcga cgtgctgccc gggcagggca tcttgacggt gatgggcaac ctgctgaccg
     7321 attcatggga aacgatctgg aagaacgccc gaccggaatg accccacccc cgccgacccg
     7381 gcgatgccaa cagcaggccc ggtggacggc gcagctgcgc cagtatcttt acgggcttgc
     7441 cggattggaa tcggcacggc gggtgctgga agtgggctgc gggtctgggg ccgtgctggc
     7501 tgattttgcc ggatggaacg gacaggttct gggggtggat cttgaccgtc ctgcgttggt
     7561 agaatcgcag ggacaggctg gttttgcgcg gctgcagcag gcggatgccg gagcactgcc
     7621 gtatgcagcc cactgttttg atttgagcct gtgtcatttt ctgctgctgt gggtggcgaa
     7681 cccggttgca acgctgcaag agatgcggcg cgttacccgc gcgggcgggg cggtgctggc
     7741 gctggcagag ccggattacg gcgggcgggt ggattatccg ccgccgctgg atgagatggg
     7801 acggcggcag gcgcaggggc tggcccggca aggcgcagac ccgctggccg ggcggaagct
     7861 gtccgggtgg ctgcatgagg ccggtttggt ggatattcaa gtgggggtgc tgggcggttg
     7921 gtgggccgag ccgcctgaag aggccgagca agcgctggag tggcaaaccc tacagcatga
     7981 tttggatggg ctggcatcag caagtgaatt ggaaaactgg cgtaaaataa atgcaacggc
     8041 ctggaaaacc gggcagcgcg tgttatacgt ccccacgttt tatggctggg ggcgggtgcc
     8101 cggataagaa aaagcgtacg gaggaaaacc atggatcact atcgggttaa accggatcaa
     8161 aaaattgagc tggatgcgtg ggatgcctcg gaaacatccc agtttaaggg gtcgaaggac
     8221 gatgccctgc cccggtttga aaaactggcg gctgaactgg gcgaactgca agaggtgatg
     8281 tttgctgaag gcaaacataa ggtgttgatt gtgctccagg cgatggacac aggtgggaag
     8341 gatggaacgg tgcgggcggt attcgggcga gtcaacccgc agggggtgcg ggtggcgaac
     8401 ttcaaagcgc cgacggcaac cgagttggcg cacgattacc tgtggcgggt gcatcaacag
     8461 gtgccgggga gaggcgagct ggtgatcttc aaccgcagcc attatgaaga tgtgctggtg
     8521 gtgcgggtgc atgggctggt gccagaggaa gtctggaaga agcggtatga gcagatcaat
     8581 gcgtttgaaa aaatgctggt tgaagaaggc acaaccatcg tgaagtttta cctgcacatt
     8641 gataaagatg aacaacgtga gcggctgctg gaacggattg atgtgcctga aaaacggtgg
     8701 aagttcaacc cgggcgacct ggaagagcgg aaactctgga aggattatat gaaggccttt
     8761 gaggcggtgc tggaacggac cagcacgcct tgggcgccgt ggtatattgt gccggccaac
     8821 cggaactggt accggaatct ggtggtggct gatgtgctgg tggagacgct gcgcgcgctc
     8881 aagatgaaat atccccagcc ccaagaggat cttacacagt accgcggtca attagggtga
     8941 aaaaagaatc tcccccttcc caagggggag atttggggta taatagcgct tgtccaaatc
     9001 ggggcgtagc gcagcccggc tagcgcgctt gcatggggtg caagaggccc tgggttcaaa
     9061 tcccagcgcc ccgactgg
//
LOCUS       DAOPVS010000080         9018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7729_length_9018_cov_53.033471, whole genome shotgun sequence.
ACCESSION   DAOPVS010000080 DAOPVS010000000
VERSION     DAOPVS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7729_length_9018_cov_53.033471"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKL11_07430"
     CDS             complement(<1..413)
                     /locus_tag="PKL11_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13472.1"
                     /translation="MRSAKEVAGGQQPRPQAKKPVLMIWLSVGVLTGLLILVFLGLSR
                     GTAVLAFLHTATPTFTTTPIPPTPTPTATLTPTQTQTSTPTPTITPTPAPPEGELFPI
                     GYSVEGRELWVVRFGNGPVVRMIVAGIHGGYEWNTV"
     gene            complement(392..1117)
                     /locus_tag="PKL11_07435"
     CDS             complement(392..1117)
                     /locus_tag="PKL11_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13473.1"
                     /translation="MMSQKPKSKTGLRIFFAALLVLGLILMAIGGILWVIPRLNLGAL
                     PNRETPPGNAVVPTQPLTEAPQPVTPLDTPAVQPTSANPPAATNPPVVVLPPTATVRP
                     MFTATPFPTNTTAPNYTPTPYCPGVSWSPRVKKGDTVKICTIYRLRLRDKVDGEDILF
                     LLPMTSGTTILEIIDGPFCGEDAWWWQVKVPANSMSNNANTNPGAPVVYTTSVMTGYV
                     KEGLDRDLLPDSQGYYLCVQPKK"
     gene            complement(1208..1714)
                     /locus_tag="PKL11_07440"
     CDS             complement(1208..1714)
                     /locus_tag="PKL11_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ13474.1"
                     /translation="MVADAVPEDITPVETPSLETTSEGLTSRGIRSIQGETQSTSDWV
                     ERGIVDVPVAALPEPEGVAGPQDFDHHLTWEDARQTTEQIPQLQQEVNAGKTGDDYSA
                     EDAAAGLDYADGRRRLYDLYYGGNAITVCKDGDQYDIIDGRHRVYAAKALGLETIPAR
                     LTEKVATS"
     gene            complement(1716..1901)
                     /locus_tag="PKL11_07445"
     CDS             complement(1716..1901)
                     /locus_tag="PKL11_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13475.1"
                     /translation="MPADQVFKITEQAVTGAVKTAETTLKRAWSILDAATEKQSPSEF
                     LSPQAIQAIQGVRKDGA"
     gene            complement(1908..4631)
                     /locus_tag="PKL11_07450"
     CDS             complement(1908..4631)
                     /locus_tag="PKL11_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNZ13476.1"
                     /translation="MGSPNLSLPGITLQKYPDLRAEAAYFGFEIPYLPDFLADHTLLG
                     QTPREKLKEVLDRFERFIIGLRKLRGSAFVLRFISDPQQGTVELYLLGRVWAAAGYAE
                     AQAIQAAHDLDVHLIGYRIPHRPLKDLPDDAGKTLSQVQCPFPHGSPLVEIRQHEELT
                     RLAVIQQDAYVIHPYWQAEGACLQPLEAMLQQTAPTVLSIYLEPIDISDAEVAAIDEA
                     AHIAQTLADVNIAVQSTTSISRRRDPAAERLSKIYSAYRQTLNEPFLVTAQVVSPDAS
                     AAWTVARVFAAEAIQGGSSSANDPASANLPSQADCLAPSNADEYAAAWNCFQNLLMSG
                     WGTSRATPGKERFPYLAGARGASAVFRLPVNLGGGIPGLAVRQLPPDFEPGPRHEQPG
                     PKEIILGRFENGGTATIPVSALTRHTLITGFTGSGKTNTVLYLLDQLWRQQRIPFLVI
                     ESAKKEYRSLLKVPGFESLLVFTLGDETVSPFRLNPFELLPGVRVEAHIGRLQACFDA
                     ALPQFGILPSIVAEALERVYRNKGWNLTDKAPAQTDVETANRRLFPTLRDLYTEVIRV
                     TDERGYAGETLHNIRAAAAGRIGNLLRGSRGRMLGGQRSIPSELLFSRPVVLELNDLN
                     EEDKSLIMMFLLTWLREWRELHPGRALQHVTVVEEAHNVVGNVQSVGASEIAADTRAK
                     AVSAFANMLSEVRAYGEGIVISDQSPEKLAPDAMRNTNLQIAHQLRDRRDREAIARAM
                     IMDEEQQDYLARLGVGEAALFRTGIQKATFIRVPEYKDEAGFDEPPADAEVEKQMAVF
                     RKQYITAVLPFDGCRFCGSPCRHRESIEPHTLDKELHEQLQAALKRFDEQPDAVYWPA
                     HWRRIAEVCAEAARRAGLEAEKDAAYCYFAHEIDFAFTRSMRESFEKATLG"
     gene            complement(4649..5437)
                     /locus_tag="PKL11_07455"
     CDS             complement(4649..5437)
                     /locus_tag="PKL11_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13477.1"
                     /translation="MSQTYPSSAPAYGSMQQPQTSSLNIWGPFAGRGARRRHIGWLMD
                     GRGDRAPELVQRIASKFNDRQIPNTNLTHENLTAKGLWVETRPYFILRRGLVTLALNI
                     SQVGRDLFISLASYIKPPISNFRVLVVGLMSLLWLFTTFAVPSMISAAADKALNGLAG
                     GLFGGGGTPDTSGLLFLLCFLGPIGLINGLLLLAFLGFSVYKWLTEKDFLAGLRVKPN
                     EFNEDDLMAMEKAVEQTVRLSLDEIGLDPNDLKATFNVSGGRVI"
     gene            complement(6092..6934)
                     /locus_tag="PKL11_07460"
     CDS             complement(6092..6934)
                     /locus_tag="PKL11_07460"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ13478.1"
                     /translation="MATLIEVLQHTLAQKDPRLSPETQRIILKEDLQAYVLDFLYNHP
                     IYRRLNFYGGTCLHVVYDLNRLSEDIDLDNGAGVDLSHLSEDLVQMFKNTFGYASTIA
                     KPQQGGQGILRITLKFPILNALGLSAYGNEALHLKVEVSHHPQTAVIQNTPVFYHGRS
                     FVPAHFSLETMLAGKMLACLERNFQRGRDGALIKGRDFYDLLWFMQKNVQPLPEKLAR
                     DGEKPYTVSSAMLALKEQISAIRVADLSVDLLPMFESRVFIEAWLEHFHANFERYLNQ
                     YVTT"
     gene            complement(6922..7572)
                     /locus_tag="PKL11_07465"
     CDS             complement(6922..7572)
                     /locus_tag="PKL11_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13479.1"
                     /translation="MKKDLLRVFEAMPYFTIEGFKQAAGIESPQTARVLLHRWAQAGH
                     ILAIKKGVYMSSRFHDQHRQDAEFSVAISAILLPNSYVSLEYILQQHSLLTETTYPVT
                     CITTRNTRTITNPLGTFWYRHIRPDWYRGFTLTEYFGIRYATANLAKALFDYLYLRPI
                     SAAFSSPRFDLAEELRLNLDEVDAESQEAFAGWVETSGSPKMRSILANFRSHVWQP"
     gene            complement(7933..8880)
                     /locus_tag="PKL11_07470"
     CDS             complement(7933..8880)
                     /locus_tag="PKL11_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNZ13480.1"
                     /translation="MKAVIFEKNATPEKLVLREVEKPVPDDNQVLIEIHAASINAADY
                     RSMRMGIIPKHRIFGADVAGRVVTAGSAVTELKPGDAVVGDLSGCGFGGFAEYAVAPA
                     GVLALKPENLSFEDAAAIPLAGVTALQGLRNLGKIQPGQRVLICGAGGGVGTFAVQLA
                     KNFGAHVTAVCGPRSVDLVRSLGADCVIDYSTEDFTRSSVRYDLILGINGSRSLSDYK
                     RLLTPCGIYVMVGGHLSQVFSAILFGPLMSLGSRKFRALAAKPNVEDTAYLLQLAAKG
                     KLKPVIDHMYPLEQTPEAMRCVSQGHTRGKVVIRIYKDQ"
BASE COUNT         2098 a         2358 c         2503 g         2059 t
ORIGIN      
        1 accgtgttcc actcataacc gccgtgaata cccgccacaa tcatccgcac cacggggcca
       61 ttgccaaaac gcaccaccca cagttcgcgc ccttcgacag aatagccaat tggaaacaat
      121 tctccttctg gtggagcagg tgtgggtgta atggttgggg ttggtgtgct ggtctgggtt
      181 tgggttggtg tgagcgtggc tgttggggtg ggcgtcggtg gtatcggcgt cgtggtaaaa
      241 gtgggcgtgg ctgtatggag gaaggcaagt acagcagtgc cgcgggacag gccaaggaat
      301 accaggatca acaatcccgt caatacacca acactgagcc agatcatcag taccggcttt
      361 tttgcctgcg gcctcggctg ctgacctcca gctacttctt tggctgaacg cacaggtaat
      421 atccttgcga atcgggcaac agatctcgat ccagcccttc tttaacatat ccagtcatca
      481 cgctggtcgt ataaacgacc ggcgcccctg gattggtatt ggcgttatta ctcatggagt
      541 tggccgggac ttttacttgc caccaccagg catcttcgcc gcagaaggga ccatcaataa
      601 tttccaaaat tgttgtgccg ctggtcattg gcagcagaaa caggatatcc tcgccatcca
      661 ctttatcccg taatcgcagc cggtaaatag tgcatatctt aaccgtatcc ccttttttca
      721 cccgcggcga ccaggaaaca cccggacagt aaggcgtagg ggtgtaatta ggggcagtgg
      781 tattggtggg gaacggcgta gccgtaaaca ttgggcgcac tgtggctgtg ggtggcagga
      841 caaccactgg tgggttggtg gccgcgggtg gattagcgga tgtaggctgt accgcggggg
      901 tatccagcgg tgttacgggc tgcggtgcct ccgtgagagg ctgggtggga acaactgcat
      961 tgcccggcgg ggtctcacgg ttgggtaaag cgcccaggtt caggcgggga atgacccaca
     1021 gaattccacc gatcgccatc aggattaaac ccagcaccaa caaggccgca aagaaaatac
     1081 gcagcccggt tttacttttc ggcttttgtg acatcattgc cagcgactgt ccggtaatag
     1141 ggcaaaagct ggcaccagca ggatgttgtt gaccacaatg cgggcagaac ataagataat
     1201 ctcctgatca ggatgtggct actttttcag tcaggcgcgc cgggatcgtc tccaacccca
     1261 acgctttggc agcataaacg cgatgccgtc catcgataat atcgtactga tcgccatctt
     1321 tgcaaactgt aatggcattt ccgccgtagt acaggtcata gaggcggcgg cggccgtcag
     1381 cataatccaa tccggctgcc gcgtcttcgg ctgaataatc atcacccgtc ttgccagcat
     1441 tcacttcctg ttgcaactgt ggaatctgtt cggtggtctg ccgggcatct tcccacgtca
     1501 ggtgatgatc gaaatcctga ggtcctgcaa caccttccgg ctcaggcaaa gcggcaaccg
     1561 gcacatctac aatacctcgc tccacccaat cgctggtgga ttgcgtttca ccctggatgg
     1621 aacggatacc ccgggatgtc aatccttctg aggttgtttc tagcgaaggt gtttcaacgg
     1681 gagtaatatc ctcaggaacc gcatctgcga ccatgttatg ccccgtcctt gcgcacccct
     1741 tgaatggcct gaatagcctg agggctcaga aattcagagg gagattgttt ttctgtggct
     1801 gcatccagga tggaccaggc ccttttcagg gttgtttcgg cagttttgac tgcgccggta
     1861 acggcctgtt cagttatttt gaagacttga tcagcaggca tgggttttta tcctaacgtg
     1921 gctttttcaa atgattcgcg catagatcgg gtaaaagcga aatcaatttc gtgagcgaaa
     1981 taacaataag ccgcatcttt ctccgcttct aacccggcgc gtcgggcggc ttcggcacag
     2041 acttcagcaa tgcggcgcca gtgggctggc cagtaaacgg catcgggttg ctcatcgaat
     2101 cgcttcaggg cagcttgtaa ttgctcgtgt aattccttat ccagggtatg cggctcaatt
     2161 gattcacgat gccggcaggg gctgccacag aaacggcagc catcaaatgg aagcaccgcg
     2221 gtgatatatt gttttcggaa aacagccatc tgcttttcaa cctctgcgtc agcagggggt
     2281 tcatcaaaac cggcctcgtc cttatattcg ggcacgcgga taaaagtggc cttctggatg
     2341 cctgtccgga acagggccgc ctcgcctacc cctaaccggg ccagataatc ctgctgttcc
     2401 tcatccatga tcatggcacg ggcaatggcc tcgcgatcac ggcggtctcg caactggtgg
     2461 gcaatttgca aattggtatt gcgcatcgca tcgggggcca acttctcagg ggattgatct
     2521 gagataacaa ttccttcgcc ataagcccgc acctctgata acatattggc aaatgcagaa
     2581 actgcttttg cccgggtgtc cgcagcaatc tcactggccc ccacagactg cacattaccg
     2641 accacgttgt gggcttcttc gaccaccgtc acatgctgca aggcccggcc agggtgcaat
     2701 tcccgccact cacgcagcca ggtcagcagg aacatcataa tgagggattt atcttcttca
     2761 ttcaaatcat taagttccag aacaaccggg cggctgaata acaattcaga ggggatgctg
     2821 cgctgcccac ccagcattct gccccggctg ccgcgcaata aattcccaat ccgtccggca
     2881 gctgcggcgc gaatattatg cagggtttcg ccagcatagc cgcgttcgtc cgtcacgcga
     2941 atgacttctg tatatagatc gcgcagggtc gggaacaacc gccgattggc cgtttcgaca
     3001 tctgtttggg cgggggcttt gtccgtcaga ttccagcctt tattgcgata cacacgctcc
     3061 agcgcctcgg ccacaatgga cggcaggata ccaaactgcg gcagggcagc atcaaagcag
     3121 gcctggaggc ggccgatatg agcttcaacc cggacgcctg ggagcaattc aaatgggttg
     3181 agtcggaacg gtgagacagt ctcatctccc agtgtaaaga ccagcaacga ttcaaatccg
     3241 gggactttta gcaggctgcg gtattctttt ttggcagatt caatcaccag gaaaggaatt
     3301 ctctgctgcc gccagagttg atccagcaga tagagcacag tattcgtttt accgctgccc
     3361 gtgaatccgg taatgagtgt gtgccgggtc agcgcgctaa ccggaatagt ggctgttcca
     3421 ccgttctcaa accgccccag gatgatttcc ttcggcccag gttgttcgtg gcgcgggccg
     3481 ggttcaaaat cgggcggaag ctgccgtaca gccaggccag gaatgccgcc tccaagattg
     3541 acaggcaggc gaaaaacagc gctagcaccc cgtgccccgg ccagataggg gaaacgttct
     3601 ttacccgggg ttgcccggga agttccccaa cccgacatca acaggttctg gaagcagttc
     3661 caggcagcag cgtattcgtc tgcattgctc ggagcaaggc aatctgcctg ggagggcaga
     3721 ttggcgctcg ccgggtcatt tgcagaactc gatcctccct gaatggcttc tgccgcaaag
     3781 acacgggcaa ctgtccaggc ggcactggca tccgggctca caacctgggc ggtgaccagg
     3841 aagggttcat tcaatgtttg gcggtaagcc gagtagattt tgctgagacg ttcagcagcc
     3901 gggtcacgcc gccgactgat actggtggtg ctctggacag cgatattgac atcggccaga
     3961 gtttgagcaa tgtgagcagc ctcatcaatt gcggccactt cggcatcact gatatcaatg
     4021 ggctcgaggt aaatacttaa tactgtcggt gcggtctgct gcagcatggc ttccagtggc
     4081 tgcaagcagg ccccctctgc ctgccagtaa ggatggatga cgtaggcatc ctgttggatg
     4141 actgccaggc gggtcaattc ctcgtgctga cgaatttcta ccagcggaga accgtgagga
     4201 aatgggcact gaacttgcga cagtgtttta cccgcatcat ctgggagatc tttcaacggg
     4261 cggtggggaa tccgatagcc gatcaggtga acatccagat catgtgcggc ctggatggcc
     4321 tgagcctctg catacccggc tgccgcccag acacgcccta atagatagag ttcaaccgtt
     4381 ccttgctgcg gatcgcttat gaatcgcagt acaaaggctg aaccgcgcag tttgcgcagg
     4441 ccaatgataa agcgttcaaa gcgatccaga acttctttta gtttttcgcg cggggtctgc
     4501 cccagcaagg tatgatcagc caaaaaatct gggagatatg ggatctcaaa gccaaaataa
     4561 gcagcttcgg cccgcagatc aggatatttt tgtagggtaa ttcctggcag agagagattg
     4621 ggtgatccca tgctggttta ctccgagttt agatgacgcg tccccccgac acgttgaacg
     4681 tagctttcag gtcatttgga tcgagcccaa tttcatccag gctgaggcga accgtctgct
     4741 ctacagcctt ttccatagcc atgagatcgt cctcgttgaa ttcattaggc ttgacccgca
     4801 gtccggccag aaaatctttt tcggtcaacc atttgtaaac gctaaaaccg agaaacgcca
     4861 gtaacaacag gccattgatg aggccaatgg ggccaagaaa acagagcaaa aagagcagcc
     4921 cggaggtatc cggggtgccg ccaccgccaa acaggccacc agccagaccg tttagagctt
     4981 tgtcggcagc tgccgaaatc atggatggca cggcaaaggt cgtgaacaac cagagcagag
     5041 acatcagccc aaccacaagg accctaaagt tgctgattgg gggcttaatg tatgaagcca
     5101 gggagataaa cagatcccgt ccaacctgac tgatatttag agccagtgtt accaggccgc
     5161 ggcggagaat aaaatagggg cgcgtctcaa cccataatcc ttttgcggtc aaattttcat
     5221 gcgtaagatt cgtgtttggg atctggcgat cattaaactt tgacgcaatc cgctgaacca
     5281 gttctggggc gcgatcaccg cgtccatcca tcagccagcc aatatgtcgg cggcgggcgc
     5341 cacgaccggc aaagggtccc caaatattga gcgaggatgt ctgcggctgc tgcatgctgc
     5401 cgtaggcagg cgccgatgat ggataagttt gtgacattga cggctggtat cccactggct
     5461 gttggatggg ctgctggggt acggggggcg gcacaggttg agattggatc ggtggttggg
     5521 atggagccgg aggtggcggg gtggaaacaa aagcattgac tggcacctgt ggtggtgatg
     5581 caaccggctg ttgtgccaga ggcgaaccac agcgcataca aaaccgataa gagggttcat
     5641 tttggaaacc gcactttggg caaatcatcc aatcctcctt gtattcaatc aagatgatgt
     5701 taagttgtga tagatctcgc tgatttcccc cacaaatcat cttcaaccat gtgcatgatg
     5761 ggggtgtgag aggttgtctt aagagattat agcacgaaga ggaaagtgaa aattcacaaa
     5821 actgaaagct gcgggtccag gcccattgag tgcatgattg ttaatctttt tcaccccaca
     5881 tatggcaccc tcagggaggc ttgacttccg acatctgaat tattccacct taatggctgt
     5941 caaacctggc tgtaagtgag cggagcgcaa ataacgacgc tactgtttgt tgatagaata
     6001 tcggtaattt atcggcagag taaagccgga cattcggggg gatgcggttc agaaatgtga
     6061 gctgttcggg caaaccgcgt gatggagggc cttatgttgt cacatattga ttcaaatacc
     6121 gctcaaagtt ggcgtgaaaa tgctccagcc aggcttcaat aaacaccctg gattcgaaca
     6181 ttggcagcaa atcaaccgac aggtcggcca ccctgatcgc agaaatctgc tccttcagtg
     6241 ccagcattgc cgaagaaacg gtgtaaggtt tttcgccatc ccgggccagt ttttccggca
     6301 gcggctggac gttcttctgc ataaaccaca gcagatcata aaaatcacgt cctttgatga
     6361 gcgccccatc ccgcccgcgc tgaaaattgc gctccaggca agccagcatc ttgccagcca
     6421 gcatggtttc cagtgagaaa tgagccggca caaaactacg cccgtgataa aacaccggcg
     6481 tgttttgaat cacggcggtc tgcggatgat ggctgacctc caccttgagg tgcagtgcct
     6541 cattgccata agcggataac cccaacgcgt tcaatatcgg gaattttagt gtaatccgca
     6601 aaatgccctg ccccccttgc tggggcttgg caatcgtgct ggcgtagcca aaagtgtttt
     6661 tgaacatctg caccaggtct tcggacagat gagagaggtc cacacccgcg ccattatcca
     6721 ggtcaatgtc ttcagacagc cggttcaggt catagactac gtgcaggcag gtaccgccat
     6781 aaaagttcaa ccgccgataa atcgggtggt tataaaggaa gtccaggaca taggcctgca
     6841 gatcctcttt gagaataatg cgctgggtct ctggcgaaag ccggggatct ttttgcgcaa
     6901 gcgtgtgctg taagacttca atcagggttg ccatacatgg ctcctaaagt tggctaagat
     6961 ggatcgcatc ttgggtgagc cactggtttc cacccaccca gcaaaggcct cctgagattc
     7021 cgcatccacc tcatccaggt tcaggcgcag ttcttcggcc agatcaaacc ggggggaact
     7081 gaaggcagca gaaatggggc gcaggtagag ataatcgaag agcgcttttg ccaggttagc
     7141 tgtggcgtaa cggatgccga agtattcggt cagggtaaag ccgcggtacc aatctgggcg
     7201 gatgtgccgg taccagaacg tgcctaatgg attggtgatg gtgcgtgtgt tccgggtggt
     7261 gatacaggtc acggggtaag tggtttcggt cagcaggcta tgctgctgga ggatgtattc
     7321 cagcgaaaca tatgagttgg gcagcaggat ggcactgatt gctaccgaga attccgcatc
     7381 ctgccggtgc tgatcatgaa accggctgga catatagacg ccttttttga tcgccagaat
     7441 atgcccagcc tgagcccagc gatgcagcaa cacccgggct gtctggggtg attcaatccc
     7501 ggcagcctgc ttgaagccct cgatggtgaa gtagggcatc gcctcaaata cgcgcagtaa
     7561 atcttttttc atattggata atatacaatt tatgtatatt atccaatata gaaatggaaa
     7621 tgtcaagcgg tttattcaaa gctgcgcttt gataagagct tctagtattt tgtgtaaccg
     7681 gaacatcagt ttgtcaggga tagatgatct gacgtattgt gtcgctctgt aaacggggag
     7741 ccggaactaa agctggtagc aaagcctttt gtctcatcca cagttagatc tcaactgcct
     7801 gcaaaagcag gcagaacgtg tcagatatat tgcaaatggc tatgactgaa atgtctttca
     7861 ctcctcttat tcttcaagct ggcctgtatt caacagtttg agccattaca ctaaatctct
     7921 gaatgccggg ctttattgat ccttgtaaat gcggatgaca actttgccac gggtgtgacc
     7981 ctggctgacg cagcgcatgg cttccggggt ctgctccaaa ggatacatat ggtcgatgac
     8041 cggtttcaat ttgccttttg cggccaactg gagcaggtaa gccgtatctt cgacgttggg
     8101 tttggctgcc agggcgcgga acttgcggct gcccagcgac atgagcggtc cgaatagtat
     8161 ggcgctgaat acctggctaa ggtgaccgcc aaccatcaca tagatgccgc atggggtgag
     8221 caggcgtttg tagtctgaga gggagcggct gccattgata cccaggatca ggtcgtagcg
     8281 cacagagctg cgggtaaaat cttcggtaga ataatcgatc acgcagtcag cgccaaggga
     8341 acgcacgagg tccacgctgc gtgggccgca gacggcagta acgtgcgcgc caaagttttt
     8401 ggcgagctgc accgcgaagg tccccactcc cccgcccgcg ccacagatga gcacccgctg
     8461 cccgggctga attttgccca ggttacgcag cccctgcaag gcggtgacac cggcaagggg
     8521 gattgccgcg gcgtcttcaa aggagaggtt ttccggcttc aatgccagca cgcccgcagg
     8581 tgcgacagcg tattctgcaa acccgccgaa cccgcagccg gagagatccc cgaccactgc
     8641 gtcacccggt ttcaactcgg tcactgccga gcctgcggtt accacgcgcc ccgcaacatc
     8701 cgcgccaaag atgcggtgtt ttgggatgat gcccatgcgc atcgagcgat aatcggcggc
     8761 attgatcgag gccgcgtgta tctcaatcag cacctggtta tcgtcaggga caggtttttc
     8821 tacctcgcgc aggacaagtt tttctggagt ggcgttcttt tcaaaaataa ctgctttcat
     8881 tattgaactc ccgttgtatt aagtgtttgc ttttttgtgg tgtgcactgg cgacgggctg
     8941 aaacctttta cgatcaacca gaccgcaaat accatttcct gcagggcaag cggggccatc
     9001 aagaacccca gcggtgat
//
LOCUS       DAOPVS010000081         8917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7844_length_8917_cov_61.662040, whole genome shotgun sequence.
ACCESSION   DAOPVS010000081 DAOPVS010000000
VERSION     DAOPVS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7844_length_8917_cov_61.662040"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..845
                     /locus_tag="PKL11_07475"
                     /pseudo
     CDS             114..845
                     /locus_tag="PKL11_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            903..1199
                     /locus_tag="PKL11_07480"
     CDS             903..1199
                     /locus_tag="PKL11_07480"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13481.1"
                     /translation="MDVRSKKKLAPRVAFGSLLEAGLISPGQALYFRRDRSRAALVKP
                     DGTLKLNGSEGSIHRLGSQLMNGSPCNGWEHWYYEDDAGEMHPIDELRAQFREK"
     gene            complement(1208..1444)
                     /locus_tag="PKL11_07485"
     CDS             complement(1208..1444)
                     /locus_tag="PKL11_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13482.1"
                     /translation="MTTQKQWQALKVQYCKHAGCQVALEAEMLLAPEQLGEQPPRINA
                     HRCSHGGLCNQLSTAACVWAGTNPTYDPFAEAGE"
     gene            complement(1466..1858)
                     /locus_tag="PKL11_07490"
     CDS             complement(1466..1858)
                     /locus_tag="PKL11_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13483.1"
                     /translation="MSNSSVPALPPASGGLFLDLARQAKLIFRLMSDRRVNFFLKLLP
                     VASVAYLFIPTDVLPILPIDDAVVLWLVNYLFVELCPNDVVTEHRAKLQALPAPAPAA
                     PTTVTHEELEIIDGEYREVEDRDAPPTA"
     gene            complement(1880..2662)
                     /locus_tag="PKL11_07495"
     CDS             complement(1880..2662)
                     /locus_tag="PKL11_07495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13484.1"
                     /translation="MTELPRVSIVTPSYNQARFLEQTIQSVLEQDYPNLEYFVMDGGS
                     TDGSLEIIQRYAPRLAGWVCEKDRGQTDALNKGFARATGQILAWLNSDDTYEPGAVSA
                     AVAALTSHPDAAAIYGEANFIDENGRTIGCFPAAQTDLPRLRRGYVHVPQQATFFRGD
                     LWRAIGPLDPSFYFAMDYDLWVRLAGRGPLVYLPGQVWANFRLHSGAKTIAADDRCWP
                     EMLRVHYRDGGSRLAPIVFKYYLRRLAAPLLNWRRRRMFRSN"
     gene            complement(2728..4188)
                     /locus_tag="PKL11_07500"
     CDS             complement(2728..4188)
                     /locus_tag="PKL11_07500"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HNZ13485.1"
                     /translation="MNAQPNFLERLIKTADLPRWGRLGYLLGVALFALTSISARYWAD
                     DYCFSGALVQSGFWQAQIDYFIETSNRFTVLPIIGISEWFGVYAIRLLPVILIFVWMG
                     ALRRVIELCSDLLDLDLDRNWVQTIIYALLFFLILLAPNRFQTIYWRSGLLTYTLPLL
                     SITWLAGLILAGSAKPVNAGRLIAVGLLAVFTAGCSETAAMLLIGGLALGLLAALLIR
                     SPRRSAWLALLGAALFGALLALVVMILSPAVRLRQANVGEPASLLLTLGLAFKFGLAF
                     IYNTLKSTPLPVLVWLIFSGALTYHFWPRAGLKRSAALRWAGLTALGGYLLIVAACIP
                     SAYAQQAYPEPRAQIVLLFPLLLTAAALAGLAALALRGWLSKSLPAVNAATTPAQRRA
                     NRVITVLLLLALLYPLWPAWQAAPEQRELAARAQQWDARDAAIRTQIAAGATQIQTVA
                     LDSIAGLEEMQPDPNHWVNVCAARYYNVDEITAQIP"
     gene            complement(4185..5129)
                     /locus_tag="PKL11_07505"
     CDS             complement(4185..5129)
                     /locus_tag="PKL11_07505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13486.1"
                     /translation="MTDSPLVSIVTPSYQQVAYLEQTLQSVLTQDYPAMEYFVMDGGS
                     TDGSVEIIRRYAPRLAGWVSEKDHGQAEAVNKGWARSRGEIIGWLNSDDLYAPGAVQR
                     AVDAFAAHPEAALVFGDVRSIDAAGNTLNIMRFGHWSLTDLMRFNIISQPGVFIRRRA
                     LEQAGFLDLSYHFLLDHQLWLRVAQHGEMIYLPQEQASARFHAAAKNLAQAARFGQEA
                     YRIVDWMQTQPALAAPFAAHRAAIEAGAHRMNARYLLDGGQPGAAFKAYARGLWRHPP
                     TVLPELHRMVFCLLSLLGLNRLGDLYRRLKARRFAGKK"
     gene            5254..5673
                     /locus_tag="PKL11_07510"
     CDS             5254..5673
                     /locus_tag="PKL11_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNZ13487.1"
                     /translation="MKSFRKELWFNVPGRRAFLNITSQVELALRESGIQEGLVLVNAM
                     HITASVFINDDESGLHHDYDRWLEQLAPHDPINRYDHNRTGEDNADAHLKRQVMGREV
                     VVAVTGGKLDFGPWEQIFYGEFDGGRRKRVLIKIIGA"
     gene            5677..6159
                     /locus_tag="PKL11_07515"
     CDS             5677..6159
                     /locus_tag="PKL11_07515"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13488.1"
                     /translation="MLFTIRPMTMADWEAVSALLAEIQDYHANALPYRFQHVDGPLRS
                     REYTQELLNDPAVKLMVSETPTGQITGFVHVVVQDTRPLPLLTPHRYAVVDTLVVGQA
                     YRRQGMGQALMLAAEDWARSLNANRIQLNVFAFNRSAQALYEELGYEITRYTMEKPLD
                     "
     gene            complement(6363..7649)
                     /locus_tag="PKL11_07520"
     CDS             complement(6363..7649)
                     /locus_tag="PKL11_07520"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNZ13489.1"
                     /translation="MASQTNAFEMAQRQFDHVADLLRLDQPVREFLRWPMREFAFRIP
                     VRMDDGTLRMFQGFRVQHNDARGPNKGGIRFHPAETIDTVRALATWMTWKCAVADIPL
                     GGGKGGVVVDPSTLSTGEKERLVRGWVQAMWKNIGPRQDVPAPDVGTTPQMMGWMMDE
                     YSKLSGVYTPGVITGKPVGGGGSLGRTEATGFGVIYTVREAMTHLKMDPKSCTAAIQG
                     FGNVAQYAAIGFIEHLGGKVICVSCWDREDRTSYTFTKKDGIDPRFLMSITDQYGTIN
                     KQAARDAGYTIESGDAWISKDVDVLIPAALEGQVNAETVQKISKGVRIVAEGANGPTT
                     PEADEVLKQRGIFNVPDFLCNAGGVTVSYFEGVQNDMNFYWTRDEVIQKLDTKMTQAF
                     ASVLNMSLSEKVYQRDAAYMVAIDRVVKAMQLRGWI"
     gene            7905..>8917
                     /locus_tag="PKL11_07525"
     CDS             7905..>8917
                     /locus_tag="PKL11_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13490.1"
                     /translation="MPTEAGKGEHHYYLIKRPAENPNDTAEIEARKHYLGIDAVRWFV
                     NKQGTILTNRIASGTVHVSLANETYEAGLGLYQLEGGARTAPIFDRPVLPNRIYPGDK
                     ITLRIYLRAIQQDTLLGGLLREMSKASLDVIAGAVGAATATGPMAVLLAAGASLTSGV
                     REILNQGKRGLTILDPEGLEITLSAADLRGPETYLLIHRGMNLAASQFRLAGNGNNIT
                     DVLYADAPLEDGAWCLFRLRREDEFGGPRPWEQEARSARAELDALMTQWSLDGVTVQE
                     VREQLKPKSGQPPSLGDRILGVIQVIRTDWVLAEREAVARAGMLKARLDLAQQAARAD
                     NFQL"
BASE COUNT         1888 a         2479 c         2884 g         1666 t
ORIGIN      
        1 caacaggtgg tggcgtggat tggaacgttt taggtcacac agagaaggct gtgcggatat
       61 ttcgacctga aatttgaaat ctcaaatctg aaattccttg atatactaga atcatgtctg
      121 acaacgacct gccgcttgat acggtgctgc agggcgactg tgtagagatt ctgcacagcc
      181 tgcccgaaaa atcggttgat ctggtgtttg ccgacccgcc gtataatctt cagcttcaga
      241 acaagctgtt ccgccccaac atgacggagg tggacgcagt ggatgaagac tgggataagt
      301 tcagtaattt tgcagagtac gatgaattta cccgccagtg gctgaccgcc tgccgccggg
      361 tgctgaaaga cagcggctcg ctgtgggtga ttggttcgta tcacaacatc taccgcatcg
      421 gcgctatttt gatggatctg ggatactgga ttttgaacga tattgtgtgg gtgaaagtga
      481 acccgatgcc caatttccgg ggggtgcgtt ttaccaacgc ccacgagacg ctgctgtggg
      541 cgtgcaaatt ccagggggcg cgctatacct tcaatcatca tgcgatgaag aatttgaatg
      601 acgatctgca aatgcgcagc gactggctgc tgccgatctg ctcgggcggc gagcgattga
      661 agcagaacgg gcacaaggcg caccccacgc agaagcccga gtcactgctc taccgggtgc
      721 tgctggcaac cacgcagccc ggcgatgtgg tgctggatcc cttctttggt acgggcacca
      781 cgggcgcagc agcttggcgg ctgcgccgtc attggattgg gattgagcgc gaaacgcgtt
      841 atgtggaact ggcgcggcag cgaattgccg ccatgcccga accgctgttt gacgatgcct
      901 ggatggatgt gcgcagcaag aagaagctgg cgccgcgggt ggcctttggc agcctgctgg
      961 aagccgggct gatttcaccc ggacaggcgc tctacttccg gcgagaccgg agccgggccg
     1021 cgctggtgaa gccagatggc acgctcaagc tcaacggcag cgagggctcc atccaccggc
     1081 tggggtcaca gttaatgaac ggcagcccgt gcaatggctg ggaacactgg tattacgaag
     1141 acgatgccgg tgagatgcac ccgattgacg agctgcgggc gcagtttaga gaaaagtaag
     1201 ccgccggtta ttcgccggct tcggcaaatg ggtcgtaggt ggggttggtg cccgcccaga
     1261 cgcaggccgc ggtggagagc tggttacaca acccgccatg cgaacagcga tgggcgttaa
     1321 tccgcggcgg ctgttcgccc aactgttcgg gcgcgaggag catttcagct tccagggcaa
     1381 cctggcagcc agcatgtttg cagtactgca ctttgagtgc ctgccattgt ttttgagtgg
     1441 tcattctaac ctcctggcaa taccattaag cagtgggcgg agcatcgcga tcttcaacct
     1501 cgcgatattc gccgtcaata atctctaatt cttcatgcgt cacggtggtt ggggcagccg
     1561 gggcaggagc aggcagcgcc tggagtttgg cacggtgctc ggtcaccacg tcattagggc
     1621 aaagctcaac aaacaggtag ttcaccagcc acagcaccac ggcatcatca atcgggagga
     1681 tgggcagcac atctgtcggg atgaacaggt aggccacgct ggctaccggc agcaatttga
     1741 ggaagaaatt gacgcgccgg tcactcatca gccggaagat cagtttggcc tgacgggcca
     1801 ggtctagaaa gaggccgccg ctggcggggg gaagtgctgg aacagaagaa ttggacatgg
     1861 gaaagatctc ctctgcgggt cagtttgaac gaaacatacg acggcggcgc cagttgagca
     1921 gcggggcggc cagccggcgc aggtagtatt tgaagacaat tggagccagg cggctgccgc
     1981 catcgcgata atggacgcgc agcatctcgg gccagcagcg atcatcggcg gcgatggttt
     2041 tggcgccgct gtgcagccgg aagttggccc acacctgccc cggcagatag accagcgggc
     2101 cgcgcccggc caggcgcacc cacaggtcat aatccatcgc aaagtagaag gacgggtcca
     2161 gcgggccaat ggcgcgccag aggtcgccgc ggaagaaggt ggcttgctgc ggcacatgca
     2221 catagccccg gcgcaggcgc ggcagatcgg tttgcgccgc cggaaagcag ccaatggtcc
     2281 gcccattttc gtcaataaaa ttggcctcgc cgtagatggc cgctgcatca gggtggctgg
     2341 tcagtgcggc gacggctgcc gaaaccgctc cgggctcgta ggtatcatct gagttgagcc
     2401 aggcgaggat ctgccccgtg gcgcgggcaa agcctttgtt cagggcatcg gtctggccgc
     2461 ggtctttttc gcagacccag cccgccagcc ggggcgcata gcgctggatg atctccaggc
     2521 tgccatcggt ggagccgcca tccatgacaa aatattccag gttcgggtaa tcctgctcga
     2581 gcaccgactg gatggtttgc tcgaggaagc gggcctggtt ataggagggc gttacaattg
     2641 aaacacgcgg cagttcagtc atctggtgtg attatagcac ggggtgcaca tttctggatt
     2701 tcaacagacg gatcggctgg ctgaagctca cgggatttgc gcggtgattt catccacgtt
     2761 gtaataccgg gcggcgcaga cattgaccca gtgattgggg tcgggctgca tttcttccag
     2821 gccagcaata ctgtccagcg caacggtctg aatctgggtg gcaccggcgg caatctgggt
     2881 gcggatagcg gcatcgcggg catcccactg ctgggcgcgg gcagccagct cgcgctgctc
     2941 tggcgccgcc tgccaggcag gccagagggg gtagagcaga gccagcagca gcagcacggt
     3001 aatcacccga ttggcccggc gctgggccgg ggtggtggca gcattaaccg ccgggagcga
     3061 ttttgagagc cagccccgca gcgccagcgc ggccagccct gccagggccg ctgccgtcag
     3121 cagcagcggg aagagcagca caatctgggc gcgcggttcg gggtaagcct gctgggcata
     3181 ggcgctgggg atgcaggccg ccacaatcag caggtagccg cccagggccg tcagtccggc
     3241 ccaccgcaga gcggccgatc gttttaaccc ggcgcggggc cagaagtgat acgtcagcgc
     3301 gccggaaaaa atcaaccaga ccagcactgg cagcggggtt gatttgaggg tgttgtaaat
     3361 gaaagccagg ccgaacttga aggccaatcc gagcgtcagc agcaggctgg cgggttcgcc
     3421 cacattggcc tgacgcagcc gcactgccgg ggagagaatc atcaccacga gggccagcaa
     3481 ggccccaaac agggcggcac ccagcaaggc cagccaggcg gagcgccggg gtgaacggat
     3541 caggagcgcg gccagcagcc ccaacgccag cccgccaatg agcagcatgg cggcggtctc
     3601 ggagcagccc gcggtaaaaa ccgccagcag gccaacggca atcaagcgcc ccgcgttgac
     3661 gggtttggcg ctgcccgcca ggatcagacc agccagccag gtgatgctga gcaggggcag
     3721 cgtgtaggtg agcagtccgg agcgccagta aatggtctgg aagcggttgg gggcgagcaa
     3781 gatcagaaag aagagcaacg catagatgat cgtctgaacc cagttgcggt ccaggtcaag
     3841 gtccaacagg tcactgcata actcaattac ccggcgcagc gcgcccatcc agacaaagat
     3901 caaaatgacc ggcagcaagc ggatggcata gacaccaaac cactcgctga tgccaatgat
     3961 gggcaggacg gtgaagcggt tggaggtttc gataaagtaa tctatctggg cctgccagaa
     4021 gccggactgc accagcgcac cgctgaagca gtagtcatcg gcccagtagc gggcgctgat
     4081 gctggtgagg gcgaataatg ccacgcccag caggtagccc agccgccccc agcggggcag
     4141 gtcggctgtt ttaatcaagc gttcaaggaa attgggttgt gcgttcattt tttgcctgca
     4201 aagcggcggg ctttgagccg ccgatagaga tcacccaaac ggttaagccc cagcaggctg
     4261 aggaggcaga agaccatgcg gtgcagttcg ggcagcactg tgggggggtg gcgccacaga
     4321 ccacgggcgt aggctttgaa ggccgcgccg ggctggccgc catccagcag gtagcgggcg
     4381 ttcatgcggt gggcaccggc ttcaatggcg gcgcggtgag cggcaaaggg tgcggccagg
     4441 gcgggctggg tctgcatcca gtccacaatt cggtaggcct cctgaccaaa gcgggctgcc
     4501 tgggccagat ttttagcggc cgcatggaaa cgggccgagg cctgttcctg cggcaggtaa
     4561 atcatctcgc catgctgggc tacccgcagc cagagctggt ggtcgagcag gaagtggtag
     4621 ctcaaatcga ggaagccagc ctgctccagg gcgcggcggc ggatgaaaac cccgggctgg
     4681 ctgatgatgt tgaagcgcat caggtcggtc agcgaccaat ggccaaagcg catgatattg
     4741 agcgtgttcc cggcggcgtc aatggagcgc acatcgccga agaccagcgc ggcctcgggg
     4801 tgagcggcaa aggcatccac agctcgctgg acggctcccg gagcatagag atcatctgag
     4861 ttaagccagc cgatgatctc accgcgggag cgcgcccagc ccttgttgac cgcctcagcc
     4921 tggccatggt ctttttcgga aacccagccc gccaggcggg gcgcgtagcg ccggatgatc
     4981 tcaacgctgc catcagtgga gccgccatcc atgacaaaat attccatggc ggggtagtcc
     5041 tgcgtcagca cggattgaag cgtctgctcc aggtaggcga cctgttgata tgaaggggta
     5101 acaatggaga cgagcggtga atcggtcatt ggtgcgatta tagcacgggg gagcgtttta
     5161 catttcacgt tccattggac gccgggcaca ggcagcccgg ttttctggta cacttggctt
     5221 gttcaaacca tctccccaga aagtgacatc ctcatgaaaa gttttcgcaa agaactgtgg
     5281 ttcaacgtgc ccggacgacg ggcatttctg aacatcacca gccaggtaga actggcactg
     5341 cgcgagagcg gcatccagga ggggctggtg ctggtcaatg caatgcatat cacggcctcg
     5401 gtcttcatta atgacgacga aagcggactg catcacgatt atgaccgctg gctggagcag
     5461 cttgcgccgc acgacccgat caaccgctac gaccacaacc gcaccggcga agacaacgcc
     5521 gatgcccacc tgaagcgcca ggtgatgggc cgcgaggtgg tggtggcagt gacgggtggg
     5581 aagctggatt ttggcccgtg ggagcaaatt ttctacggtg aatttgacgg cggacgccgc
     5641 aaacgggttt taatcaagat catcggagcg tgaagtatgc tgtttaccat tcgccccatg
     5701 acaatggccg actgggaagc cgtgagcgcc ctgctggctg aaattcaaga ctatcacgcc
     5761 aatgcgctgc cctaccgctt ccagcatgtg gatgggccgc tgcgcagccg cgagtacacc
     5821 caggaactgc tgaacgaccc ggccgtaaaa ctgatggtca gtgaaacacc caccggacag
     5881 atcactggat ttgtgcatgt agttgtgcag gatacccgcc ccctgccgct gctgacgccg
     5941 caccgctacg cggtggtgga tacgctggtg gtggggcagg cttaccggcg acagggcatg
     6001 ggccaggcgc tgatgctggc ggctgaagat tgggcgcgtt cgctgaacgc caatcgcatt
     6061 cagttgaacg tctttgcgtt caaccggtcg gctcaggcgc tatacgaaga gctggggtat
     6121 gagatcacgc ggtacacgat ggagaagccg ctggattgag cggtgtaatt ctaaaaaaac
     6181 ccaaagaaaa ctttaacccg attttaatgc aattgttttt aaaacgtgat aatataacaa
     6241 agtcaagcgg ctgggtgccc ccctcacccc gccgcttgac gcttaaacag ccagcaccca
     6301 aaacgacaaa taaaatcccc cgcccaaagg cgggggattc gtttttttga aaaagctgaa
     6361 gtttagatcc agccgcggag ctgcatggcc ttgaccacgc gatcgatcgc caccatgtaa
     6421 gcggcatcgc gctggtagac cttttcgctc aggctcatgt tgagcacaga ggcgaaggcc
     6481 tgggtcatct tggtgtcgag cttctggatg acttcgtcgc gggtccagta gaagttcata
     6541 tcgttctgca cgccttcgaa gtaggagacg gttacgccac cggcattgca gaggaaatcg
     6601 gggacgttaa agataccgcg ctgcttgagg acttcgtcag cctcgggggt ggtggggcca
     6661 ttggcgcctt cagccacaat gcggacaccc ttgctgatct tctggacggt ttcagcattg
     6721 acctggccct cgagggcggc ggggatgagc acatccacgt ctttgctgat ccaggcatcg
     6781 ccggattcga tggtgtagcc agcatcgcgg gcagcctgct tgttgatggt gccgtactgg
     6841 tcggtgatgg acatcaagaa gcggggatca atgccgtctt tcttggtgaa ggtgtaggag
     6901 gtgcggtctt cgcggtccca gcaggagacg cagatgacct tgccgcccag gtgttcaatg
     6961 aagccgatgg cggcatactg cgccacgttg ccaaagccct gaatggcagc cgtgcagctc
     7021 ttgggatcca tcttgagatg ggtcatggct tcgcgcacgg tgtagatgac gccgaagcca
     7081 gtggcttcgg tgcggcccaa cgagccgccg ccgcccacgg gtttgcccgt gatgacgccg
     7141 ggggtgtaaa cgccgctcag cttggagtat tcgtccatca tccagcccat catctggggg
     7201 gtggtgccaa catcaggcgc cgggacatcc tggcgagggc caatgttctt ccacatggcc
     7261 tgcacccagc cgcggacgag gcgttctttt tcgccggtcg agagggtgga aggatctacc
     7321 accacaccac ctttaccgcc acccaggggg atgtcggcaa cggcgcattt ccaggtcatc
     7381 caggtggcca gggcgcgcac ggtgtcaata gtttcagcgg ggtggaagcg gatgccgccc
     7441 ttgttggggc cgcgggcatc gttgtgctga acacggaagc cctggaacat gcgcagggtg
     7501 ccgtcgtcca tgcggacggg gatgcggaag gcgaattcgc gcatcggcca gcgcaggaat
     7561 tcgcgcacgg gctggtccag acggagcaaa tcggccacat ggtcgaactg ccgctgtgcc
     7621 atttcaaacg cgttggtttg ggaagccatt gaaattctct cctgttgggt ttgaaaatcg
     7681 aaagattttt gccatcagaa tgccggataa cgcttgcctg ctgggggagc ggatgagcga
     7741 ctctcctcca ccggattgtt acatgaatag gttaaccgat agctcccttt gcgtcaatat
     7801 gatgaaaatc ggttttgtgt ggtgatgtac ctccgcatgg ttgtatcatc tgtcagtttt
     7861 aactgtataa tatttttaac cattcgcgtt gaggaggatc agccatgcca accgaggccg
     7921 gaaaaggtga acaccattac tatctgatca agcgtccggc agagaaccct aacgatactg
     7981 ccgagatcga agctcgcaaa cattacctgg ggattgacgc cgtgcgctgg tttgtcaaca
     8041 agcaggggac cattctgacc aaccggatcg ccagcggaac ggtgcacgtc tcgctggcca
     8101 acgagactta cgaagccggg ttggggctgt atcaactgga gggcggggcg cgcactgcgc
     8161 caatctttga ccgcccggtg ctgcccaacc ggatttaccc cggcgataag attacgctgc
     8221 ggatttatct gcgcgccatc cagcaggata cgctgctggg cgggctgctg cgcgaaatgt
     8281 cgaaagccag cctggatgtg attgccggag ccgtaggcgc cgccacagcc acggggccca
     8341 tggcagtgct gctggcagcc ggcgccagtc tgacgagcgg cgtgcgcgag attttgaacc
     8401 agggcaaacg aggcctgact attctggatc ccgaaggatt agagattacc ctgtcggcgg
     8461 ctgatttacg cggccccgaa acctacctgc tgatccatcg cggcatgaac ctggcagcca
     8521 gccagttccg gctggcgggc aatggcaata acatcaccga tgtgctgtac gccgatgctc
     8581 cgcttgaaga cggtgcctgg tgcctcttcc ggctgcggcg agaagatgag tttggcgggc
     8641 cgcgcccctg ggagcaggaa gctcgcagtg cccgcgccga actggacgca ttgatgaccc
     8701 agtggagcct ggacggggtg accgtgcagg aggtgcgcga acaactcaaa cccaagagcg
     8761 gtcagccgcc cagcctgggc gaccgaattt tgggcgtcat tcaggtcatc cgcaccgatt
     8821 gggtgctggc agagcgcgaa gcggtggcgc gcgccgggat gctgaaggcc cggctggacc
     8881 tggcgcagca agccgcccgc gccgataact tccagct
//
LOCUS       DAOPVS010000082         8872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7901_length_8872_cov_55.775207, whole genome shotgun sequence.
ACCESSION   DAOPVS010000082 DAOPVS010000000
VERSION     DAOPVS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7901_length_8872_cov_55.775207"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..455
                     /locus_tag="PKL11_07530"
     CDS             93..455
                     /locus_tag="PKL11_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13491.1"
                     /translation="MSDSLDLETRRRRSSGWIGGIVLILVGIIILTKNLTGFQLQNWW
                     ALFILIPAVSSLMNAWNTYRENGNQFSRGVTGPLIGGLLLSFLVVMFVLGLDFGKFWP
                     IFLILAGLAALASAFQKE"
     gene            480..>1357
                     /locus_tag="PKL11_07535"
     CDS             480..>1357
                     /locus_tag="PKL11_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNZ13492.1"
                     /translation="MIRITREDALEYHHLDGVPGKLSVVPSKPLDSQRDLSLAYTPGV
                     AYPVLEVEQDPANAYSYTNKGNLVAVISNGTAILGLGHRGALASKPVMEGKGVLFKKF
                     AGIDVFDIEVDVLDPDLLIEVVAAIAPTFGGINLEDIRSPECFRIEQTLQARLDIPVF
                     HDDQHGTAIILGAALMNALEITNRKIKDIKVVFSGAGAAGIACANQLVKLGVPREHIW
                     MCDAAGLVYHGRAELMFPEKEIYAQGEQAGTLAEALVGADVFIGLSAANIVAPEMLKP
                     MRSNPIIFAMANPDPE"
     assembly_gap    1358..1457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1641..2081
                     /locus_tag="PKL11_07540"
     CDS             1641..2081
                     /locus_tag="PKL11_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13493.1"
                     /translation="MSEKLPHPSTPQTPAHKRQVFWQILLPIILAAAAVIAIVVLATI
                     STQGGGETVAVWSDVSLIFLLIPLTFGIFIFLALTVAIIYGVQRLTNILPVYARIVQL
                     HAERLTLVFKQWANKAADPAIAANSAKAGWDKFWQKISRSNPTQ"
     gene            2091..2303
                     /locus_tag="PKL11_07545"
     CDS             2091..2303
                     /locus_tag="PKL11_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13494.1"
                     /translation="METTPNWKARTMIIGAIVGSLAGLAAAYVLISRAEQDDKAQAKL
                     TAGDGVKVGLGLLGILRLLADSSDKH"
     gene            2345..3367
                     /locus_tag="PKL11_07550"
     CDS             2345..3367
                     /locus_tag="PKL11_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNZ13495.1"
                     /translation="MPSDSSGLIRFVIAGTLRQDFILLPNRQSYAGMPGGSLLYAAAG
                     MNIWTRDIALVGRVGQNYPQEWIERLNKYQFDTRGIHKLPHDLDARAFYAYPDLNTRI
                     LSDPVMQYSRAGLPYPKELLGYANPTPQIDSRNQPTDYTIRLTDFPNDYLDATAAHLA
                     PLDFLTHTLLPSALRQGHVNTITLDPGAGYMQPAFWDDIPRLIKGLTAFLTSEEKLRT
                     LFGGRSSDLWEMAEAIAAYGCDFVVVRRGVQGQYLYDHSSRTRWVVPAYPGRGVDPTG
                     AGDAFCGGFLVGYRERYSPLEAALQGNISASLVIEGSGPFYAMDTLPGLAAARLEALR
                     SSVRKA"
     gene            complement(3373..5106)
                     /locus_tag="PKL11_07555"
     CDS             complement(3373..5106)
                     /locus_tag="PKL11_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ13496.1"
                     /translation="MTDTTPTTTTTTFSVLDQHLYSLTVLSNQLQEMLLRLAPSALPG
                     SMHEQVIQLTDGVTKIRQYVNVLDEERRSLRDLASIGQVINSTLELDEVLQVVMDTLI
                     RLTGAERGFLMLRGADGELGLRVARNWEKGTIDASETAISSTVINRVVTTGVPILTTN
                     AQEDPRFGGQQSVIAYNLRSILCVPLRVKSEITGVIYADNRIRSGLFTRSDLDLLAGF
                     ANQAAVAIENARLFDSVRRTLAEVTELKDLMDNVFASIASGVLTANIDERVILCNRAA
                     ETILGRESSILVGNLLTQTLEPLTEMLSPRFKHVVQTDEPVLGWEVSPNLPDRGHVDL
                     RLSLTPLKDVGRQTQGVAIVLDDLTEKKRLEAQRRLFERMVSPAVIEQLDPECLQLGG
                     RRAEITVLFADIRGFTSFSEGVTPEELVQVLNRYLAAAAEAVLGEDGTVDKFLGDAVM
                     AWFNAPILQEDHTLRAIRAALRIRDAIQALHREFPPRFQLAFGVGIHVGEAVLGLVGT
                     EKRMEYTAIGDSVNTAKRVQENAGPSQILITESAYQLVASQIEVRPVEPIQAKGKRAP
                     IPAYELLSLRQ"
     gene            complement(5125..7431)
                     /locus_tag="PKL11_07560"
     CDS             complement(5125..7431)
                     /locus_tag="PKL11_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNZ13497.1"
                     /translation="MAIAIIAASRLEATNWAENLDLATTAAVLGGILGLAIGKSRFSR
                     RTAAWLAVIYSILVVPWILGTIVGEGVLWLERLASLGARLSLTLSQLIYNQPIRDPLL
                     LIVIMVLAFWLLSLIGGYRLVRYGRPWVPLILIALILMLIEFFSASLIRSAWYSAGFA
                     FVVVCLIAQLYFRQSYRQWEEQNVLIDSDTEFFRGRAALITVSVLVVVAWLLPFTLRA
                     LVPGSIAQQEVRDKWTGLQERLNNVFGSFQGPARVEVWTFGEVMTLSTGQPSSDLPLF
                     RVETPIETLKGTTYYWRARSYDSYRDNHWQNTITNRLDIIPNVNTVVLPPWAPRLEGE
                     FAFEAQTNLLVLYSPGMATTISRPVEVVARELDDGSLDFVAMQANPMVRAGDRYTVTA
                     SLGSPTIAQLRVAGDEYPDWVAQKYLQIPSDLPQSILDLSAEITASYDTPYEKTVAIT
                     NYLRRTIKYSETIPEPPKNADPLEWFLLEYKQGFCQYYATAEVMLLRAAGVPARLVVG
                     YAPGDLQGDERTFQVRAKHSHAWPEVYFPNIGWVEFEPTSGLNPIARPLGDVADLYPG
                     DGGGLDDDLPIPDDEELPDDNPNLQPDDAAAGPGGSSAYQYNQPLLIAAGQFGLVSEA
                     LLALVLIYQRMIHKLIRWQPLPIIVEALFRRLGLKRPAWLNRLAEEARLSPIERYFRR
                     IGWALRRLKHPGAPSLTPAEQVAALNKIYPQGAELARILLDEYERAVYSPYSPDLARA
                     QQAQTDLRQMVIRSWWENLLTDSREPVD"
     gene            7568..8569
                     /locus_tag="PKL11_07565"
     CDS             7568..8569
                     /locus_tag="PKL11_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ13498.1"
                     /translation="METIWQIEIQITLFLQSLGDWLTPIMKGFSFLGNEEFYLIIMPF
                     LYWCVNSTWGARVAIMMLLSNGLSAPLKLVFHSPRPYWVDPQVQGMISETSFGLPSGH
                     AQNAASIWGALAVSLKKRWVTIGVIVVVFLIGLSRIFLGVHFTSDVLGGWLVGGLLVL
                     GLIKLEKPIVEWFKRRSFEQSLIVALVSSLLIILVCLLIELAWNGWEMPAAWIQNAVA
                     DTGIEPDPLNLNGIIGVAGTWLGMLGGISWFYRRYGMFYAAGPGWKRIVRYFVGIAGV
                     LLLWYGLGKIFPRDYDILSFSLRYLRYMLVGLWVAAAGPWTFIRIGLAGKPSARRSK"
     gene            8580..8801
                     /locus_tag="PKL11_07570"
     CDS             8580..8801
                     /locus_tag="PKL11_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNZ13499.1"
                     /translation="MRRAGNFLAGFIIGGLISAVLGLIFAPSSGPQLRQRIVDYVQQT
                     RADVEKAAKDRRTELEIELQELRKPEPKA"
BASE COUNT         2023 a         2191 c         2569 g         1989 t
ORIGIN      
        1 tccggttgac acgatgaggg tcttcctccc ggcgatactt tgtggtacaa acaattagca
       61 gatgagattg ctaaaattga aaggatttcc ccatgagcga ctctttagat cttgaaactc
      121 ggcggcgccg cagctccggg tggattggcg gaattgtgct gattctggtt gggatcatca
      181 tcctgaccaa aaacttgacc ggattccaac tacaaaactg gtgggcgttg ttcatcctga
      241 tcccggcagt cagttcgctg atgaatgcct ggaataccta ccgggaaaac ggcaatcaat
      301 tcagccgggg tgttaccggg ccattgattg gcgggctgct actcagtttt ctggtggtca
      361 tgttcgtttt agggcttgat tttgggaaat tctggcccat tttcttaatt ctggcgggct
      421 tggctgcgct ggcatcggct tttcaaaaag aataaccatc acaacataag ggaacaatca
      481 tgatccgcat cacccgagaa gacgctttgg aataccatca cctggacgga gtgcctggca
      541 agctgtcggt agtgcccagc aagccgctgg acagccagcg tgacttgagc ctggcgtata
      601 cgcccggtgt ggcttacccg gtgctggagg ttgagcaaga tcctgccaat gcctactctt
      661 acactaataa aggcaatctg gtggctgtca tctcaaacgg tacggccatt ttggggcttg
      721 gacatcgcgg ggcgctggcg tctaaaccgg tgatggaggg gaagggtgtt ctgtttaaga
      781 aatttgcagg gatcgatgtc tttgatattg aagtggatgt gttggatccc gacttattaa
      841 tcgaagttgt ggcggcgatt gcgcctacct tcggcgggat taacctggaa gacatacgat
      901 ccccggagtg cttccgaatt gagcagaccc tgcaagccag gctggatatt ccagtctttc
      961 atgatgacca gcatggcacg gctatcatcc tgggggcagc gttgatgaat gcgctggaga
     1021 ttaccaaccg gaagattaag gatattaagg tggtgttttc gggggcagga gccgccggga
     1081 ttgcctgtgc caatcaactg gtcaagctgg gtgtcccgcg agagcatatc tggatgtgcg
     1141 atgcggcggg actggtatat catgggcgag ccgaattgat gtttccagag aaggaaatct
     1201 atgcccaggg cgaacaggcc ggaacgctgg cagaagcact ggtaggtgct gatgtgttta
     1261 tcggtttatc ggctgccaac atcgtcgccc cggagatgct aaagccgatg cgcagcaatc
     1321 cgattatttt tgcaatggcc aacccggacc ctgagatnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnctg gaatttggcg tcgattatat tgtgcccaag ccgctggacc
     1501 cacgagtgtg tttgtgggag gcgccagcag tggcaaaggc tgccattcag agcggcgccg
     1561 cccggcgggc gctggacctg gatacttatg tggaagtgct gcaaaaaaga atcgcaaaac
     1621 cgtgctaaaa tagtaaccat atgagcgaaa aactgcccca cccaagcacg ccccagacgc
     1681 ccgcccataa gcggcaggtg ttctggcaga ttttactgcc gatcattctg gcggcagccg
     1741 cagtgattgc tattgtagtg ctggcaacga tctcaactca ggggggcgga gagacggtgg
     1801 ctgtctggtc ggatgtatcg ctcatttttt tgctgattcc gttgacgttt ggaattttca
     1861 tcttcctggc gctgacagtg gcgatcattt atggcgttca gcgcctgacc aacatcctgc
     1921 ctgtttatgc ccggattgtg cagctgcacg ccgaacgatt gaccctggtg ttcaaacagt
     1981 gggccaacaa agcggctgac ccggcaattg cggcgaacag tgccaaagcc ggctgggata
     2041 aattctggca aaaaatttca cgttcaaacc ccacgcaata gaggttcacc atggaaacaa
     2101 cccccaattg gaaagccagg acaatgatca ttggcgcaat cgtaggttcg ctggcaggtc
     2161 tggcggcggc ttatgtactg atttctcgag ctgaacaaga tgacaaggcg caggccaaat
     2221 tgaccgccgg tgatggcgtt aaggttgggc tgggcttgct gggaattttg cgattgctgg
     2281 cggacagcag cgataagcac taagcccccc cttttaagac ccataaccca gaccggggga
     2341 catgatgcca tctgacagct ctggtttaat tcgatttgtg attgccggta cgctgcggca
     2401 ggattttatt ctgctgccca accggcaatc ttacgcgggc atgccggggg gcagtttact
     2461 atacgccgct gccgggatga atatctggac aagagatata gccctggtgg gaagagtagg
     2521 tcaaaattac cctcaggaat ggatcgaacg gttaaataag tatcaatttg acacgcgcgg
     2581 tattcataag ctgccacatg acctggatgc cagggctttt tatgcctacc ccgatttgaa
     2641 tacccgcatc ttgagcgacc cggtgatgca gtattcacgc gccgggctgc cctaccctaa
     2701 agagttgctg ggttacgcca accctacccc gcagattgac agccgcaatc aacctacaga
     2761 ctacaccatc cgcttgaccg acttccccaa cgattacctg gatgctacgg ctgcgcacct
     2821 ggcgccgttg gattttctga ctcacaccct gctgccatca gcgctccgac aggggcatgt
     2881 caacactatc acactggacc caggggcagg gtatatgcag cctgccttct gggatgatat
     2941 tccgaggctg attaaagggt tgactgcgtt tctgacatca gaagaaaagc tgcgcacctt
     3001 gtttggcggg cgaagctcag atttatggga aatggcagaa gccatcgctg cgtatggctg
     3061 tgattttgta gtagttcggc gaggcgtgca gggacagtac ctttatgacc acagcagccg
     3121 cacccgctgg gttgttccag cctatccggg acgcggggtt gaccccaccg gagcaggcga
     3181 cgccttctgc gggggatttc tggtgggcta ccgagaacgg tacagtccgc tggaagctgc
     3241 cttgcagggc aatatttcgg cctcgctggt gattgaaggc agcggaccat tttacgccat
     3301 ggacacgctg ccagggctgg cagcagcccg gctggaagcg ctgcgcagtt cggtgcgaaa
     3361 agcctgaacg ggttactgcc gcaaggaaag cagctcgtat gcggggatgg gggcgcgctt
     3421 gccttttgcc tggatgggtt ccaccgggcg gacttcaatt tgcgacgcta cgagctggta
     3481 agctgattcc gtgattaaga tctgactggg accggcgttt tcctgcacac gcttggcggt
     3541 gttgacgctg tctccgatgg cggtgtattc catgcgtttc tcggtgccga ccagaccaag
     3601 cacggcctcg ccgacatgga tgccaacgcc gaaagcaagc tgaaagcgcg gtgggaattc
     3661 ccggtgcagg gcttgaatgg catcgcgtat gcgcagcgcc gcgcggattg cccgcagggt
     3721 atgatcttcc tggagaattg gggcattgaa ccaggccatg acggcgtcgc ccaggaattt
     3781 atcaaccgta ccgtcttcac ctaagaccgc ctcggcagcg gccgccagat aacgattgag
     3841 gacctggacc agttcttcag gggtgacacc ctcactgaaa ctggtaaagc cgcggatatc
     3901 agcaaacagg acggtaattt cggcgcggcg gccccccaac tgcaaacact cggggtccaa
     3961 ctgttcaatg acggcgggcg aaaccatgcg ctcaaagagg cggcgctggg cttcgagacg
     4021 ttttttctcc gtcaggtcat cgagaacgat ggccacaccc tgggtttggc ggcccacatc
     4081 tttgagcggc gtcagactga ggcgcagatc aacatgaccg cggtccggaa gattggggct
     4141 gacctcccat cccaataccg gctcatcggt ttgaaccaca tgtttgaaac ggggagagag
     4201 catctctgtc aatggttcga gcgtttgagt taaaagattg cccaccagga tagaagactc
     4261 gcgtccaaga atggtctcgg cggcgcggtt acacaggatt acgcgctcat ctatgttcgc
     4321 tgtgaggacg ccgctggcga tggatgcaaa gacattatcc atcaaatctt tcagttcggt
     4381 tacttcagcc agcgtgcgcc ggacggaatc aaacaggcgg gcattctcaa tagccacagc
     4441 cgcctgattg gcaaaccctg ccagcaaatc caggtcgctg cgagtaaata agccactgcg
     4501 gatgcggtta tcagcataaa tcacgccggt gatttcgctt ttgacacgca gcgggacgca
     4561 gaggatagaa cgcaggttgt aggcaatgac gctttgctgt ccgccaaaac ggggatcttc
     4621 ctgggcgttg gtggtcagaa taggcacgcc agttgtgacc acacggttga tcactgtgct
     4681 gctgatggcg gtttcagagg catcaatagt gcctttttcc caattacgag ccacgcgcag
     4741 gcccagttcg ccatcagcgc cgcgcaacat cagaaaaccg cgctcggcac cggtgaggcg
     4801 gatcagagtg tccataacca cttgcagaac ttcgtccaat tcaagggtcg agttaatcac
     4861 ctggccgatt gaagcaaggt ctcgcagact gcggcgttct tcatctaata cgttgacata
     4921 ttgacggatt ttggtcacgc catcggttaa ttgaatcact tgctcgtgca ttgagccggg
     4981 caaggctgag ggcgccagac gcaggagcat ctcttgaagc tggttggaca agaccgttaa
     5041 ggaatagagg tgctggtcca gaactgaaaa ggttgtggtt gtggtggttg gtgttgtatc
     5101 agtcaaacta ccctcccatg ccggttaatc caccggctca cggctgtcgg ttaagagatt
     5161 ttcccaccag gagcgaatga ccatctggcg tagatcggtt tgggcctgtt gagcgcgagc
     5221 caggtcaggt gaataggggc tgtaaactgc ccgttcatat tcatccaata agatgcgggc
     5281 cagctcggcg ccctgtggat aaattttgtt gagtgcggcc acctgttcgg caggcgttag
     5341 agaaggtgca ccggggtgct tgaggcggcg cagcgcccag ccaatccggc ggaaatagcg
     5401 ttcaattggc gagaggcgcg cctcttcagc caggcggttc agccaggcag ggcgcttgag
     5461 gccaaggcgg cggaagagcg cttcaacaat aatgggcagg ggctgccagc ggatgagttt
     5521 atgaatcata cgctgataga tcaatactaa agccaggagc gcttcagata ccaggccaaa
     5581 ctgcccagca gcaatcagca ggggttggtt gtattgataa gccgatgacc cacccgggcc
     5641 agctgccgca tcatcgggct gcaggtttgg attatcatcg ggcaattctt cgtcgtcggg
     5701 aatgggcaag tcatcgtcca gtccgccgcc gtcgccaggg taaagatcgg caacatctcc
     5761 cagaggacgg gcaatcgggt tcagcccgga tgtgggttca aactcaaccc agccgatatt
     5821 cgggaaatag acttcgggcc aggcgtggct atgcttggca cggacctgga aagtgcgctc
     5881 atcgccctgc aagtcaccgg gagcgtagcc caccaccaaa cgagccggga cgcccgccgc
     5941 ccgcaagagc atcacttcgg cagtggcgta atactggcaa aaaccctgct tgtactccaa
     6001 caagaaccat tccaaaggat cggcattttt aggcggttcg gggatggttt cagaatactt
     6061 gatagttcgg cgcaggtaat tggtgatggc taccgttttt tcgtagggcg tgtcgtaaga
     6121 agcggtaatt tcagcagata aatccaaaat actctggggc aaatctgaag ggatctggag
     6181 gtatttttga gccacccaat cgggatattc atcgccagcc acccgcagtt gggcaatggt
     6241 ggggctgcca agcgaggcgg tgacggtata acggtcaccg gcgcgcacca taggattggc
     6301 ctgcatggcg acaaaatcca gagagccatc atccagttca cgggcaacga cttcaaccgg
     6361 gcggctgatg gtggttgcca tgccggggga atataaaacc agaagattgg tttgtgcttc
     6421 aaacgcaaac tcaccctcca agcgcggcgc ccagggtggc aggacaaccg tgttcacatt
     6481 tggaatgata tcaaggcgat tggtaatggt attctgccag tgattatctc tgtagctgtc
     6541 atagctgcgg gcccgccagt aataggttgt gcctttgagg gtttcaattg gggtttctac
     6601 cctgaatagt ggaaggtcag aactgggctg cccggttgac agggtcatta cttcaccaaa
     6661 ggtccagact tccacccggg cagggccctg gaacgagcca aagacgttat tcaagcgttc
     6721 ctggagaccc gtccacttat cgcggacttc ttgctgggcg attgaaccgg ggacaagtgc
     6781 acgcagggtg aacggcagaa gccaggcaac cacaaccagg actgacaccg tgatgagggc
     6841 ggcgcgcccc cggaagaatt ctgtatccga atcaattaag acattttgtt cttcccactg
     6901 gcgataggac tgccggaaat acagttgagc aatcaaacag acaacaacaa aggcaaaccc
     6961 ggcagagtac caggctgaac ggattaaact ggcgctgaaa aattcaatca acatcagaat
     7021 gagggcaatc aaaatcaacg gcacccaggg acgcccatag cgcaccaaac ggtagccgcc
     7081 gattaatgag agcagccaaa acgccaaaac cataataaca atcaacagca gggggtcacg
     7141 gatgggttga ttgtaaatga gctggctgag tgtcagactc aaacgagcgc caaggctggc
     7201 taaacgctcc agccagagga caccctcgcc gacgatagtt cctaaaatcc aggggacgac
     7261 aaggattgag taaattacag ccagccaggc agcagtgcgt cgtgaaaagc ggcttttacc
     7321 gatcgcaagg cctaatattc cacccaacac agcggcggtt gtcgcaaggt caaggttttc
     7381 agcccagtta gtggcttcta gccgagaagc ggcaataatc gcaatagcca tccagcatat
     7441 tgccgccgga acatcccacc aacgattctg tttcttatcc atggtttgag tgtacaataa
     7501 aagagattgg cgcgtcaata tcaagcgact gacggcaatc gctagaaatc attccgcgga
     7561 gaacactatg gaaacaatct ggcagatcga gattcaaatt acgttattcc tgcaatcttt
     7621 aggggattgg ctgacaccga ttatgaaggg gttttcgttt ctaggcaatg aagaatttta
     7681 cctcataatc atgccttttt tatactggtg tgtaaactca acctggggag ccagggttgc
     7741 aatcatgatg ttgttgagca acgggctctc ggcgccgctc aagctggttt ttcacagccc
     7801 gcggccttac tgggttgatc cgcaggtgca gggaatgatc agcgagacaa gttttgggct
     7861 gccatctgga catgcacaga atgcagccag catctggggg gcattggctg tgtcgctgaa
     7921 aaagcggtgg gtgactatcg gtgtgattgt ggtggtattc ctgattggtt tatcccggat
     7981 tttcctgggg gtgcacttta ccagcgatgt gctgggcggc tggctggttg ggggcttgct
     8041 ggtactaggg ttgattaagt tagaaaagcc aattgtcgaa tggtttaagc ggcgctcttt
     8101 tgagcaatct ctcattgttg cgctggtctc atcactgttg atcattctgg tctgcctgct
     8161 gattgagctg gcgtggaacg gatgggaaat gccagccgcc tggattcaga atgcggtagc
     8221 tgatacggga attgagcctg acccgttgaa tctcaatggg attattgggg tcgccggaac
     8281 gtggctggga atgctgggcg ggatcagttg gttttaccgg cgttacggca tgttctatgc
     8341 tgccggaccg ggttggaagc ggattgtgcg ttattttgtt ggaattgctg gtgttttgct
     8401 gctatggtat ggattgggca agatttttcc gcgagattac gatatactta gctttagcct
     8461 gcgttacctg cggtatatgc tggttggcct gtgggtagcc gcggcaggcc cctggacatt
     8521 cattcggatc ggactggctg ggaagccctc tgccaggcgg tcaaaataag gaggttgtca
     8581 tgcgtcgagc aggaaatttt ttggccggtt ttatcatcgg cggtttgatc agtgctgtgc
     8641 tgggtttgat ttttgcgcct tcttccgggc cacaactccg gcagcgaatt gttgattacg
     8701 ttcaacagac gcgggcggat gttgaaaaag ccgccaaaga tcggcgcacc gaacttgaga
     8761 ttgagcttca ggaactgcgc aaaccagagc ccaaagccta gagagggata ccttcctgta
     8821 atatgtgctt taacagaagg ggcgccctga tgggtcgttt ttgtattcca ag
//
LOCUS       DAOPVS010000083         8865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_7911_length_8865_cov_45.112145, whole genome shotgun sequence.
ACCESSION   DAOPVS010000083 DAOPVS010000000
VERSION     DAOPVS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7911_length_8865_cov_45.112145"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKL11_07575"
     CDS             complement(<1..523)
                     /locus_tag="PKL11_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13500.1"
                     /translation="MRFFDLVSIIASNLSRRKGRVVLTAIGVVIGTAAVVLLVSLAIG
                     LQQSATSQLYGISDLRRIEVYPNYDTGGGGGGGVVYSDKGQIPGSSEAKLLTPKALEE
                     LAALPGVEYVIPRDYLYYETHIVSGKLETWGQIIGIGTDDLANLGDTAETGVTTLERG
                     TAIIGGWAARNFYD"
     gene            complement(535..1995)
                     /locus_tag="PKL11_07580"
     CDS             complement(535..1995)
                     /locus_tag="PKL11_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13501.1"
                     /translation="MKRTWPVLILIAALTLFSLMNTAPTLAQTETPTPTETPTLPPPA
                     TTRPILIIDSYGSGEGAIRPGNEFELGVRLVNIGGEKARNIIATFTSTDFYPKDTGGV
                     VATPVLAPSADINFHQPMLADTDLWGRTLGVITMTLSYTNNDGTISYSENFVLTIDIQ
                     TPNWVAATATPTISPLVPRPQIVVDSYASDVDPLQPGTIFNLTLNLRNLGNADARAVS
                     MVLGGGVTLDASGTPQPGGVSGSGSDLSTFAPLGSSNVIYLGDLLAANTTSTTMQLIV
                     NVSANPGAYPLKLAFVYTDPKNNRLLDDQVITLLIYSLPQLDVNFYTDPGIFFSNSMN
                     MLPLQVTNLGRKSAVLGNITVTSENADISNNTALVGALEPGGYFTLDAQVMPYAAGPL
                     DLTVTINYTDDFNMVRTVTQTLTVNVEEMYIEPIPGENGEPPIIEPEPETFWDKVLRF
                     FKGLFGLDSAEPTPEPIPAETFPEDQPIEPVPGGKG"
     gene            complement(2023..2754)
                     /locus_tag="PKL11_07585"
     CDS             complement(2023..2754)
                     /locus_tag="PKL11_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13502.1"
                     /translation="MDDTAFIQFRQLRKVFMMGRTMVQALAGIDLDIPAGAFAVIMGP
                     SGSGKSTLLYLLGGLDRPTSGTITVGGESVDKMDENALAIFRRRRVGFIFQSFNLISS
                     MTAQENVAFPMRFAGIPPAQRHKRSLALLEQVGLQKRTRHRPTELSGGQQQRVAVARA
                     LVNDPALILADEPTGNLDTSSGYAIMQLLSDLHRSGRTVVVVTHDPRMKHFATHFVYL
                     LDGRIVSEAEFEAASQFESQQAAEA"
     gene            complement(2759..3538)
                     /locus_tag="PKL11_07590"
     CDS             complement(2759..3538)
                     /locus_tag="PKL11_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNZ13503.1"
                     /translation="MTEMDIELNHEEKPRRMRWDWLLPVFLRPKRTLTEVVAQEHGVW
                     LVPLLVLSILVILNVVAGASARRAAVMEAQSGELPYGFEYWTPEEQEQYFSALARSAS
                     PVNLYVFPILGGVAGIWITWFLLGSILHLSLTLAGSRSSNAGALNLTAWASMPIAIRL
                     IVQAIAVLASGHVIQSAGLSGFAPAEAPFWSAVLKLVDIYLIWQFVLLLIGVLPLSGL
                     SRRKAWVATLIAFLIVLTLRALPAFISAKLSGLSTGGFFYF"
     gene            3677..4690
                     /locus_tag="PKL11_07595"
     CDS             3677..4690
                     /locus_tag="PKL11_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ13504.1"
                     /translation="MHASAHTPPNAILRRMIPGLMLGVLVLIGLALLGDLKQVGAHLQ
                     SFNWAVFPLALLLTLFNYTLRFFKWHYYLGQIGVHTLPLLESARLFVAGFPLAVTPGK
                     VGEVLKGVWLEQRTGVPVARGVSVVVAERISDGLAVLALSTLGVIAYPRYWPAFALVL
                     GLLLTLVIGSQIRPAAYWALGLGERIPFVKRFIPALREFYEGSFMLFRPGAMLVGVGL
                     GTISWLGEGIGFYLILLGLGLPPGLELLGLAVFVLAFSTVVGAASALPGGLGAAEASI
                     AGMLALLYGLDPATASAATLLIRLATFWFGIALGLLTWLFSRDLLGFTPSPKRQHDDD
                     PQS"
     gene            4671..5327
                     /locus_tag="PKL11_07600"
     CDS             4671..5327
                     /locus_tag="PKL11_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HNZ13505.1"
                     /translation="MTTLKVDFHVHTRWSRDSLNQLPQLLQSAERRGLDRIVITDHNT
                     IAGALAARQMAPERVIVGEEVLTTRGELLAAFVTEEVPKNLPPLEAIARLRAQGAFIS
                     VSHPFDRQRSGWALPDLLEIAPLVDAIEIFNARCYTAQQNLEAQQFAAEHHLPGTAGS
                     DAHAPFEVGRALLALPAFDDPASLRAALPQARLFARRSLPLVHLLSFYARMTHKSRIR
                     "
     gene            5429..6574
                     /locus_tag="PKL11_07605"
     CDS             5429..6574
                     /locus_tag="PKL11_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ13506.1"
                     /translation="MRIKTIPLLVTTLLLLGLILTACSPKPTATLPSEIIIGVYEPMT
                     GFQAEGGQQTMQGINLAYEQDSSALGLPVRLVLMDNRSDRYESASVVNRLIEQEHVIA
                     IIGSYGSSNAIAGGAVAEAAGIPVMGCSPTSPLVTTGRDYYFRVSYIDPFQARVMAQY
                     AVDQEISRIAIIYNITQAYSVGLANDFRQSWIELTGSADGIVATVAYSDGDEDFTDQI
                     TLSMTAEAIYIPGYFGDAARLMQQARDLGYTGRFLGSDGWEAPELLSIGGEAVEGAVF
                     TTHFSAEVPDSPLAQQFVTQFEARYGEPPSAFAALGYDAYRLVLDSIERAQSADPAAI
                     RDAMAATRNFAGVTGSITIDDSGNAVKNAVLMVVRDGAFEFLALAKP"
     gene            6640..6840
                     /locus_tag="PKL11_07610"
     CDS             6640..6840
                     /locus_tag="PKL11_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13507.1"
                     /translation="MEELVKLVSKKTGLSADMSKVAIETVLGFLKKKLPAPIAAQVDA
                     VLKGDAAGVAGTIGSLLGGKKK"
     gene            6910..8673
                     /locus_tag="PKL11_07615"
     CDS             6910..8673
                     /locus_tag="PKL11_07615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNZ13508.1"
                     /translation="MTAYTQTRWDLADLYPAADSPELEATFTQLRDQVQAFAALRPQL
                     TESITTADFLALIRKLEAIDYQASRLGGFAHLQFAGDTQNQKAVALLGRVEQFLAGLM
                     NETLFFSLWWKGISDQTAERLMAESGDYRYWLEEMRHFKPHTLTEPEEKIINIKDVTG
                     ISALTHLYDAITNRYVFKMEVDGEVREMTRGELMVYARHADPDLRAKAYQQLYQVYGD
                     DAALLGQIYQYVVRDWRSENVELRHHAEPISARNLGNDIPDAVVNTLLEVARKNVTLF
                     QRFFRLKARLLGVERLRRYDIYAPVTQAEKEYPFGQAAELVLDAYRKFDPKVEALARR
                     VFEQNHLDGEVRKGKRGGAFCATPLQDVTPWVLLNYQGRADDVETMAHELGHAVHSML
                     AAHHSIFTAHSCLPLAETASTFGEMLLVDRMLAEETDPAVRRDIIFRQLDGAYGTIQR
                     QSFFAMFERQAHDLIQNDAAVDEISEAYLQNLRDQFGDAVELSDEFKYEWVSIPHFYH
                     TPFYVYAYAFGQLLVLALYQQYKREGESFKPRYFKILSTGGSEAPVRLLADAGIDIYK
                     AEFWQGGFDVLADLVDQLEKM"
BASE COUNT         1777 a         2687 c         2501 g         1900 t
ORIGIN      
        1 ggtcataaaa gttacgcgcc gcccagccgc caatgatggc ggtgccgcgc tccagggtgg
       61 tcacgccggt ttcagccgta tcgcccaggt tcgccaggtc atcggtgcca attccaataa
      121 tctgtcccca ggtttccagc tttcctgaaa caatgtgggt ttcataatat agatagtcgc
      181 gcgggatcac gtattccaca cccggcaggg ccgccagttc ttccagcgct ttgggcgtca
      241 gcagcttggc ttcggatgaa cccgggattt gacccttatc gctgtagaca acgccgccac
      301 caccgccccc gccagtgtca tagttggggt acacctcaat ccggcgcagg tcgctgatgc
      361 cgtaaagctg gctggtggcg ctttgctgca ggccaattgc cagcgagacg agcagcacga
      421 ctgccgccgt gccaatgacg actccaatgg ccgtcagaac cacccgcccc ttgcggcggc
      481 tcaggttgct ggcaatgatg ctgaccaggt caaagaaacg catacgtgca gcctttatcc
      541 tttgccgccc ggaacgggtt caatcggctg atcttcgggg aaggtctctg ctgggatggg
      601 ttcgggagtc ggctcggcgc tgtccagccc aaacaggccc ttgaagaatc gcagcacctt
      661 atcccagaaa gtttcgggtt ccggctcaat aatgggcggc tcgccatttt caccggggat
      721 cggttcaata tacatttctt caacatttac cgtcagcgtt tgtgtcaccg tgcgaaccat
      781 gttgaaatca tccgtatagt tgatggtcac cgtcaggtca agcgggccag cggcataggg
      841 catcacctgg gcatccagcg taaagtagcc gccgggttcc agtgcgccga ccagcgcggt
      901 gttattgctg atgtcagcgt tctctgaggt cacagtgatg ttgcccagca ccgcgctctt
      961 acgccccagg ttggtcacct gcaagggcag catattcatt gaattggaga agaaaattcc
     1021 cgggtctgtg tagaagttca catccaactg gggcagcgaa tagatcagca gcgtaatcac
     1081 ctggtcatcc agcaggcggt tgttcttcgg gtcggtgtac acaaacgcca gcttgagtgg
     1141 gtaagcccca gggttggctg atacattgac aatcaactgc atggtggtgc tggtagtgtt
     1201 tgccgccagc aggtcaccca gatagattac attggaactg cccagcggcg caaaggttga
     1261 tagatcactg ccgctgcccg aaaccccgcc cggctgcggt gtgccagaag catccagcgt
     1321 cacgccgccg cccagtacca tcgagactgc ccgcgcatcg gcgttaccca ggttacgcag
     1381 gttcagcgtc agattgaaga ttgtgccggg ctgcagtgga tccacatcac tggcgtaact
     1441 gtccaccaca atctgcgggc ggggcaccag cggcgagatg gtgggcgtag ccgtagctgc
     1501 cacccagttg ggcgtctgaa tatcaatcgt cagtacaaaa ttttcactgt agctgatggt
     1561 gccatcatta ttcgtgtagc tcaacgtcat ggtaatcacg cccaacgtcc ggccccacag
     1621 gtccgtatct gccagcatcg gctgatggaa gttgatatct gccgagggcg ccagcacggg
     1681 cgtggcaacc acaccgcccg tatccttggg gtaaaagtcg gtagaggtga aggtggcaat
     1741 aatgttgcgg gctttttcgc cgccaatgtt caccagccgc acccccagtt caaactcatt
     1801 gcccgggcgg atggcacctt cgcctgatcc atacgagtcg atgatcaaaa tcgggcgcgt
     1861 ggtagccggc gggggcaggg tgggggtttc tgtcggagta ggggtttcgg tttgcgccag
     1921 ggtgggcgca gtgttcatca ggctgaagag ggtcagcgca gcaatcagga tgagaacagg
     1981 ccaggtgcgt ttcataaaga gtgtttctcc agtgtgtcag ccttaagcct cggcggcttg
     2041 ttggctttcg aattgagagg ccgcttcaaa ttcggcctca ctgacaatgc ggccatccag
     2101 caaatagaca aagtgggtgg caaagtgttt catacgcggg tcgtgagtga ccaccacaac
     2161 ggtgcgtccg ctgcggtgca ggtcggataa taattgcata atcgcatagc cgctggacgt
     2221 atccaggttg ccggtgggct catccgccag gatcagcgcc gggtcattga ccagcgcccg
     2281 ggctacagcc acgcgctgct gctgcccgcc cgaaagctcg gtggggcggt ggcgggtgcg
     2341 tttttgcagc cccacctgct ccagcagcgc cagcgagcgc ttgtggcgct gcgccggtgg
     2401 aatgcccgca aaccgcatcg ggaaggccac attctcctgg gcggtcatgc tggagatgag
     2461 gttgaaggat tgaaagatga agcccacgcg ccgccgccgg aagatggcca gcgcgttttc
     2521 gtccatcttg tccaccgatt ctccccccac ggtgatcgta ccggaagtgg ggcgatccag
     2581 ccctcccagc aggtacagca gggtgctttt gcccgaaccc gaaggcccca tgatgacggc
     2641 aaacgcgccc gccgggatat ccaggtcaat ccctgccagg gcctgtacca tcgtgcgccc
     2701 catcatgaaa accttgcgga gctgccggaa ttgaatgaaa gcagtatcgt ccattggctt
     2761 agaagtagaa gaatccgccg gtcgaaaggc cgctcagctt ggcgctaatg aaggccggca
     2821 gcgcccgcag cgttagcaca atcaaaaatg caatcagggt ggcaacccag gccttgcggc
     2881 gtgaaagccc cgaaagcggc agcacaccga tcagcagcag aacaaactgc cagatcaggt
     2941 aaatatccac cagcttgagc accgcgctcc agaatggcgc ttctgccggg gcaaagcccg
     3001 agagccccgc gctctgaatc acgtgccccg aggccagcac ggcaatggcc tgcacaatca
     3061 accggatggc aatcggcata ctggcccagg cggtcaggtt gagcgccccc gcattggaac
     3121 tgcggctgcc tgccagtgtc aatgaaaggt gtaaaatgct gcccagcaaa aaccaggtaa
     3181 tccagatgcc tgccacgccg cccagaattg ggaagacata caggttcacc gggctggcag
     3241 atcgcgccag cgcgctaaag tactgctcct gttcttcggg cgtccaatat tcaaacccgt
     3301 agggcagttc gccgctctgc gcttccatga cggccgcgcg gcgcgccgat gctcccgcca
     3361 ccacattcaa gatcaccagg atgctgagga ccagcagcgg caccagccag acgccatgtt
     3421 cctgagccac aacttcggtc agggtgcgct tggggcgcag aaataccggc agcagccaat
     3481 cccagcgcat ccggcggggc ttttcttcgt gattgagttc aatgtccatt tcggtcatgg
     3541 gaaatcctct tgaagtgcag gcctcggcgc ctgccaggca taaaagtaga atgtaccaaa
     3601 tataccatag acgtcgttct gtcaacaaaa gttccaacaa atcacaaact gcacagcacc
     3661 gggtataatc aaccctatgc atgcctctgc ccacacgcca cccaatgcca tcctccggcg
     3721 gatgatcccg gggctgatgc tgggtgttct ggttctcatt ggcctggccc tgctggggga
     3781 tctgaagcag gttggcgccc atctgcaaag cttcaactgg gccgtttttc cgctggcgct
     3841 gttgctgacc ctgttcaatt acacgctccg ctttttcaag tggcactatt acctggggca
     3901 aatcggcgtc cacaccctgc ccctgctgga aagcgcccgg ctctttgtgg cgggctttcc
     3961 gctggcggtc acccccggca aggtcggtga ggtgctcaaa ggcgtctggc tcgaacagcg
     4021 aaccggcgtc ccggtggcgc gcggcgtttc agtggttgtg gcagaacgca tcagcgatgg
     4081 tctggcggtg ctggcgctct ccaccctggg cgtcattgcc tacccgcgct actggccggc
     4141 ctttgccctg gtgctgggcc tgctgctcac cctggtcatt ggttctcaga tccgcccggc
     4201 ggcgtactgg gccctgggcc tgggtgaacg gataccattt gtcaagcgct tcatcccggc
     4261 gctgcgtgaa ttttatgaag gcagctttat gctcttccgg cccggcgcca tgctggtggg
     4321 cgttggcctg ggaaccattt cctggctggg cgagggcatt ggcttctatt tgattttact
     4381 tgggctgggg ctgcctcccg gtctggaact gctgggactg gcggtctttg tgctggcttt
     4441 ttccaccgtt gtgggtgcgg cttcagcgct gccgggcggt ctgggcgccg ctgaggcttc
     4501 catcgccggc atgttggccc tgctctatgg cctggacccc gccaccgcct cggctgccac
     4561 gctgctcatc cggctggcaa ccttctggtt tggcattgcg ctggggctgc tcacctggct
     4621 cttctcgcgt gatctgctcg gcttcactcc atctccaaag aggcagcatg atgacgaccc
     4681 tcaaagttga ttttcacgtc cacacccgct ggtcccgcga cagcctgaac caactgccgc
     4741 agcttttgca atcggctgaa cggcgcgggc tggatcgaat tgtgattact gaccataaca
     4801 ccatcgccgg ggcgctggct gctcgccaaa tggcccccga gcgcgtaatt gtgggcgaag
     4861 aggtgctgac cacccgcggt gaactgctgg ctgcttttgt gaccgaagaa gtgcccaaga
     4921 acctgccccc attggaagcc atagcccgcc tgcgcgccca gggagccttt atcagcgtct
     4981 cacacccctt tgaccgtcag cgcagcggct gggccctgcc cgacctgctg gaaattgccc
     5041 cgctggtcga tgccatcgaa atcttcaacg cccgctgtta taccgcacag cagaatcttg
     5101 aggcgcagca gtttgccgcc gaacatcacc tgcccggcac cgccgggtcc gacgcccatg
     5161 ccccgtttga ggtggggcgg gcgctgcttg ccctgcctgc ttttgatgac cccgccagcc
     5221 tgcgagctgc cctgccgcaa gcccgtctct ttgcccggcg ctcgctgccc ctggttcacc
     5281 tgctctcctt ctatgcccgt atgactcata aatcccgaat ccgctgagat tttgcgcttg
     5341 ctttaagccc atttgttgta tactaataag cacccagatt catcaaaaat taatcttcct
     5401 gcacaaaggg gcaaccaggc gagaggtcat gcgaatcaaa acaatccctt tgttggtcac
     5461 tacattgctg ctgctggggc tgatcttaac agcctgctcc cccaaaccaa ccgcaaccct
     5521 gcccagtgaa attatcatcg gcgtctatga accgatgacg ggctttcagg ctgagggcgg
     5581 gcagcaaacc atgcagggca tcaatctggc ttatgagcag gacagcagtg cactgggtct
     5641 gccggtgcgc ctggtattga tggataaccg cagcgaccgc tatgaatctg cgtctgtcgt
     5701 caaccgcctg atcgagcagg aacatgtgat tgccatcatc ggcagctacg gctcatccaa
     5761 tgccattgcc ggcggggccg tggccgaagc cgccggcatc ccggtgatgg gctgctcacc
     5821 caccagcccg ctcgtcacca ccgggcgaga ctattacttc cgcgtcagct acattgaccc
     5881 cttccaggcc cgggtgatgg ctcagtatgc ggtggatcaa gaaattagcc gcatcgccat
     5941 tatttacaac attacccagg cctattcggt tggcctggca aacgattttc ggcagtcctg
     6001 gattgaactg acgggcagcg ccgacgggat agttgccacg gtggcctaca gcgatggcga
     6061 tgaagacttt accgaccaaa tcaccctttc gatgactgcc gaagcgatct acatccccgg
     6121 ctattttggc gatgccgctc ggctgatgca gcaggcccgc gacctgggct ataccgggcg
     6181 cttcctcggc tcggacggct gggaagcccc cgaactgctc tccattggcg gcgaggcggt
     6241 tgagggcgca gtcttcacca cgcacttctc tgccgaagtc cccgattcgc ctctggcgca
     6301 gcagtttgtc acccagtttg aggctcgtta cggcgaaccc cccagcgcct ttgccgccct
     6361 gggatacgac gcctaccggt tggtgctgga ttcaattgag cgtgcccaat cggcagaccc
     6421 cgctgccatt cgagatgcca tggctgccac gcgcaatttt gccggggtta ccggcagcat
     6481 cacgattgat gattcgggaa acgccgtcaa aaacgcggtg ctgatggttg tgcgggatgg
     6541 cgcatttgaa tttttggcgc tggcaaaacc ctgatgtaat ttgattttca atgcttcttg
     6601 gattatactg gttctacatt ttcaaaccgg gaggttacaa tggaagaact ggtcaagctt
     6661 gtgtccaaga agaccggcct gagcgccgat atgtcgaagg ttgccatcga aaccgtgctg
     6721 ggcttcctca aaaagaagct gcccgccccc atcgccgcac aggtagatgc tgttttgaaa
     6781 ggtgatgccg cgggtgtagc tggcaccatt ggcagcctgc ttggcggcaa gaaaaaataa
     6841 tcttctgcgt ctattcaccg gcagccctaa aagctgccgg aatttacatc tacttcagga
     6901 gtacgttgaa tgactgccta tactcaaacc cgttgggatc tggccgatct ctacccggct
     6961 gctgacagcc ccgaactgga agccaccttt acccagctgc gtgaccaggt gcaggcattt
     7021 gccgctctgc gcccgcagct caccgaatcc atcaccaccg ccgattttct tgcgctgatt
     7081 cgcaagctcg aagcgattga ttaccaggcc tctcgcctgg gtggttttgc ccacctgcag
     7141 tttgctggcg atacccagaa tcaaaaggcc gttgcccttt tgggccgcgt ggagcagttc
     7201 ctggcaggcc tgatgaacga gaccctgttc ttcagcctgt ggtggaaggg catctcagac
     7261 cagaccgccg aacggttgat ggccgaatcg ggtgattatc gctactggct cgaggaaatg
     7321 cgccacttca aaccgcatac cctcaccgag cctgaagaaa agatcatcaa tattaaagat
     7381 gtcacgggca tcagcgccct cacccatctg tatgacgcca tcaccaatcg ctacgtcttt
     7441 aaaatggaag ttgacggcga agtgcgcgaa atgacccgcg gtgagctgat ggtctatgcc
     7501 cgccatgccg accccgatct gcgcgccaaa gcctaccagc agttatatca ggtctatggc
     7561 gacgacgccg ccctgctggg gcagatctac caatacgtgg tgcgcgattg gcgcagtgag
     7621 aacgtggagc tgcgccacca tgccgaaccc atctcggccc gcaacctggg caacgacatc
     7681 cccgatgccg tggtgaatac cctgctggaa gtggcccgca agaacgtcac cctcttccaa
     7741 cgcttcttcc gcctcaaggc gcgtttgctg ggcgttgagc ggctgcgccg ctatgatatt
     7801 tatgccccgg ttacccaggc cgaaaaagaa tacccctttg gtcaggctgc cgaactggtg
     7861 ctggatgcct accgcaagtt tgatcccaaa gtggaagccc tggctcgccg cgtttttgaa
     7921 cagaaccacc tggacggcga agtccgcaag ggcaagcgcg gcggcgcctt ctgcgccacc
     7981 ccgctgcaag atgtgacccc ctgggtgctg ctgaactatc agggccgcgc tgatgatgtg
     8041 gaaaccatgg cgcacgagct gggccacgca gttcattcca tgctggctgc ccaccacagc
     8101 atcttcaccg cccactcctg cctgcccctg gctgaaaccg cctccacctt tggtgaaatg
     8161 ctgctggtgg accgcatgtt ggccgaagaa accgatcccg ccgttcgccg cgatatcatc
     8221 ttccgccagt tggatggcgc ctacggcacc attcagcggc agtcgttctt tgccatgttt
     8281 gagcgccagg cccacgacct gattcaaaac gacgccgcag tggatgagat ctctgaggca
     8341 tacctccaaa acctgcgcga ccagtttggc gatgcggttg aactgagcga cgaattcaag
     8401 tacgaatggg tttccattcc gcacttctac cacacaccat tctacgttta tgcctatgcc
     8461 tttgggcagc tccttgtcct ggccctctat cagcagtaca agcgcgaagg cgagagcttc
     8521 aagccgcgct atttcaagat cctctccacc ggcggctctg aagcgcccgt gcgccttctg
     8581 gcagatgccg gcattgatat ctacaaagcc gagttctggc agggcggctt tgatgtcctc
     8641 gccgatctgg tcgatcagct cgaaaaaatg taagcccgca actcctttca cggaacttaa
     8701 ggcggtctgt ttgctccggc aggcagaccg cctcttgttt tgcttagttc cccaaactca
     8761 ttataaatac atgacataga ttataaaggg gtgtgctata atattttcat taattctcta
     8821 atctaccgct aatttagtcc aatggttcag atttggatca ttgga
//
LOCUS       DAOPVS010000084         8323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_8565_length_8323_cov_57.038462, whole genome shotgun sequence.
ACCESSION   DAOPVS010000084 DAOPVS010000000
VERSION     DAOPVS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8565_length_8323_cov_57.038462"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKL11_07620"
     CDS             <1..201
                     /locus_tag="PKL11_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13509.1"
                     /translation="ALATVTIYLPGQNPQTLPVKNTNQSGFAVVEYAVGDLPEKELIQ
                     VRVLVSVGGKQVETQTWFRIWW"
     gene            208..>2405
                     /gene="dnaE"
                     /locus_tag="PKL11_07625"
     CDS             208..>2405
                     /gene="dnaE"
                     /locus_tag="PKL11_07625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ13510.1"
                     /translation="MSFVHLHLHTQYSLLDGFTNIKRLMTRVKELGMPAVAITDHGTM
                     FGVIEFFNAAKEVDIKPIIGLEAYLAPRRMTDRDARLDKQSHHLLLLAQNMTGYRNLL
                     KIASAAQLEGFYYHPRIDHEFLAAHSEGLICTTGCMAAEVPRIIIDRGAEAARPRLDW
                     YYEVFGRDRFFFELQQHPIPELGIINKTLLELGKSYGARYIATNDAHYINREDARLQD
                     IMLAIQTGSLLNDPNRMRMNDDSYYLRTPDEMKELFAEVPEAISNTLLIAEQCEVDLS
                     SKEYHLPLFPVPEGHTAQTYLRQLCDEGLRRRYGARADDPEVLRRLEYELSVINQMGF
                     DAYFLIVWDLCRHARSEGIWYNARGSAAGSQVAYVLDITLVEPLEHGLIFERFLNPGR
                     ISMPDIDLDFQDDRRGEMMHYCAVTYGDDHVGQIITFGTLGGRAAVRDVGRVMDIPLS
                     EVDRISKLIPNVPSKAIPIPEAVATIPELKEIYDSADYLRNLIDTAALMEGVVRNAGT
                     HAAGVVITDRPMVDYVPLHRATSGSEDSPIKTVVQFEMSILDSLGLLKVDFLGLSSLT
                     VMQRACALIEQRHSQHFDLGNIPLDDPAAFELMGLGHTAGVFQLEGSGMTRFLMQMHP
                     QNLANVIAMVALFRPGPMEFIPAYIRRMHGEERVEYLDPALEPIFGETFGIAIYQEQL
                     MRASVDLAGYTLPESDDLRKAISKKQKDKLLKHREKFIHGASDRGMPTETA"
     assembly_gap    2406..2505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2506..4044
                     /locus_tag="PKL11_07630"
     CDS             <2506..4044
                     /locus_tag="PKL11_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNZ13511.1"
                     /translation="SHAADYGVIAVQTAYLKAHYPEEYMTALISASKSDSDKVAFYVA
                     DCRALGIDVLPPDINTSCWDFTIEDRPDQRPAIRFGMGAVKNVGQAPVELILQGRADG
                     PFKDLNDFAHRVDLRQVGKRSLECLTRVGALDAFGQRRALLDGLDRIIAVSSSHFKAL
                     QSGQLSFFGAITGVEEQISLPAAGEVDTREQLEWEKELLGLYVSDHPLSPYMGAIRRR
                     VTHTASTLGEARHKAVVTVAGMVTRLRPHQTKAGKPMGFATIADLEGSIELILFPRTW
                     AEFGKLIRPDVVITVQGSVDAEGGDPKVLVDRVELLNLETLPAEAPAAAPDEIPWDED
                     GPPPPPEPDDWHLQPPPAVEPLWMAPQAADVQPAPPANPPVEPPPAAKTVPPAAQAEP
                     ALPPPPVVSEPAPPPYEPLHYIPPPEESTANPAANPQRMIRLVLRATGDKQRDGRRMR
                     RLYGMLVSSPGRDKFAFMIFESGRRYLMEFPNDSTGITNALIAALGEQIGSDNVIVET
                     IPVH"
     gene            4256..5422
                     /locus_tag="PKL11_07635"
     CDS             4256..5422
                     /locus_tag="PKL11_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNZ13512.1"
                     /translation="MKNRSLLMIPGPIEFDPAVLAALGAPTTSHLAPEFIEAFGQALE
                     RTRALFQCPDGQPFVLAGTGTLGMDTACANLIEPGDAALVVNTGYFGDRIGAILERYG
                     AQVSQVRAAPGGHPALAAVEQALQGGGFKVMTITQVDTSTGVLADVRGLAALGQRYGA
                     LVIVDGVCSLAGEDLNMGAWGVDVAFTASQKAVGVPPGLALLVAGPRALAALQARRTP
                     VGSYYSDWNNWLPVMQAYEARKAAYFGTPAVNLVFALNVSLGQILQEGLAARFARHER
                     ISRACKAGIAALGLGQVPLNADIAAHTMTAPRFPAGVSGADLLPRVAQAGVTLAGGLH
                     AEIKNEYFRIGHMGAVTSGDVLATLGAIESALAACGHKFQPGAALAAAQQVFLG"
     gene            complement(5479..7455)
                     /locus_tag="PKL11_07640"
     CDS             complement(5479..7455)
                     /locus_tag="PKL11_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13513.1"
                     /translation="MKPPHILNLLDEAARQHIPDERNLLPGILARIQPQEKKQMKLKI
                     VWTVLAVLLGLGLITGAGYALYRVFFHDPGIDAVQEAGLVSPVEQSAAPTLQPQITTP
                     PALPETPVQILGQTQTLQGVTWTLEWLQINTSGLQFGFSMSGSPAEPQLPAVQVSSAT
                     DAPRGQVLWLQQDGGQWAGIYRLNQYLPAGSTDLTLLLGLTGQTGPAFHLNGLDVQPG
                     QMPTSQYIYSAQIDARERTLEAVVLGPQTARARVCATGEPAPDWAALTIQADLGGLGE
                     PPRISAGLHQVLPVPQAPACADLLFDLPASQQPETLHLTLTPTVGTAWEFFAEAPAWP
                     ESAPHTVIQPQPTATPIAAQTVGQLTATLDWVYADTQRVAMQMRFEGWQPSYTLWDAT
                     LTTADGKTLDFNIGQPSSEDGSTYLLIFDASHKLSGDSTQLTLNLPVQDANDWQTTLA
                     AFQFQVTVPVYPGIQLAGVQAVTANGLTLRLERAEITPSYTSAILCYDKPTHGPGSDW
                     GVSAGTQLEGSQTQTTLLNYALLQDADAQLFLDPQRNFTPSQPADRCIELGFPIGSVQ
                     QPGGVLTLVIPGLELSMPESIPADAWAAAQEKLAAQGIQIEQYTFSANGGGGGGWNIL
                     QRPPGMSDEQVYQLTKEALGYTYPGPWVFELPLP"
     gene            complement(7452..8003)
                     /locus_tag="PKL11_07645"
     CDS             complement(7452..8003)
                     /locus_tag="PKL11_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ13514.1"
                     /translation="MQQEQNAIQRLKRGDISGLEYLVTRYQVRAMRAAFLILQDEAQA
                     EDVVQGVFLHLYHHIRRFDETRPFGPYLMRSVVNAALNQAQSNARLRPIDPDAPMDLE
                     QWLADTPDPESLIEAAELRQQVLQALKRLAPRQRAAIVQRYYLQMSEQEMAQNLQAAP
                     GTVKWLLNAARARLRALLERSER"
     gene            complement(8133..>8323)
                     /locus_tag="PKL11_07650"
     CDS             complement(8133..>8323)
                     /locus_tag="PKL11_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13515.1"
                     /translation="SGIDMDLFFIRGPWYSNRIEVLPDNGIGLLCFSRPFPRDEDNSQ
                     WLDWEGYGTYKFDEGWIE"
BASE COUNT         1491 a         2587 c         2606 g         1539 t
ORIGIN      
        1 gccctggcaa cggtgacgat ctacctgcca ggccagaacc cgcaaaccct gccggtcaag
       61 aataccaatc agagcggctt tgcagtggtt gagtatgccg ttggcgacct accagaaaaa
      121 gaacttatcc aggtccgtgt gctggtctca gtggggggca agcaagtaga gacgcaaacc
      181 tggttccgga tctggtggta gggcgtcatg tcgtttgttc atctccacct ccacacccag
      241 tactcgctgt tagacggctt caccaacatt aagaggttga tgacgcgggt taaagaactg
      301 ggcatgcccg cggtagccat caccgaccat ggcaccatgt tcggcgtgat tgaattcttc
      361 aacgccgcca aagaagtgga tatcaaaccg atcatcgggc tggaggccta cctggcgccc
      421 cgccggatga ccgaccgcga cgcccggctg gacaagcagt cgcatcacct gctgctgctg
      481 gcgcagaaca tgacgggcta ccgcaacctg ctgaagattg ccagcgccgc ccagttggaa
      541 gggttttatt accacccccg cattgaccac gaatttttgg cggctcattc tgaagggctg
      601 atctgcacta ccggctgcat ggctgccgag gtcccgcgca tcatcattga ccgcggcgct
      661 gaggctgccc gcccccgcct ggactggtat tacgaagtct ttggccgcga ccgctttttc
      721 tttgaacttc agcagcaccc catccccgag ctgggcatca tcaacaaaac gctgctggaa
      781 ctgggcaaaa gctacggggc gcgctacatc gccaccaacg acgcgcatta catcaaccgt
      841 gaagatgccc gcctgcaaga catcatgctc gccatccaga ccggctcgct gctcaacgac
      901 cccaaccgga tgcgcatgaa cgatgatagc tactacctgc gcaccccgga cgagatgaaa
      961 gagctgttcg ccgaggtgcc cgaggccatc agcaacaccc tgttgattgc cgagcagtgc
     1021 gaggtggacc tctcctctaa agaatatcac ctgccgctct tccccgtgcc cgaaggccac
     1081 accgcccaga cctacctgcg gcagctctgt gatgagggcc tgcgccgccg ctacggcgcc
     1141 cgcgcggatg atcccgaagt gctgcggcgc ctggaatacg aactgagcgt cattaaccag
     1201 atgggtttcg acgcctactt tttgattgtg tgggacctgt gccgccatgc ccgctcagag
     1261 ggcatctggt acaacgcccg cggctcggcg gcgggctcgc aggtggccta tgtgctggat
     1321 atcaccctgg tggagccgct ggaacacggt ctgatttttg agcgcttcct caatccgggg
     1381 cgtatctcca tgcccgatat tgacctggac tttcaggatg accgccgcgg cgagatgatg
     1441 cactactgcg ccgtcacata cggcgacgac cacgtcgggc agatcatcac ctttggcacg
     1501 ctgggcggcc gcgccgccgt gcgtgatgtg ggccgggtga tggacattcc cctctccgag
     1561 gtggaccgca tcagcaagct gatccccaac gtgccgagca aggccatccc cattcccgag
     1621 gcggtggcca ccatccccga actgaaggaa atttacgact cggccgatta tctgcgcaac
     1681 ctgattgaca ccgccgccct gatggaaggc gtggtgcgca atgccggcac ccacgccgcg
     1741 ggcgtggtca tcaccgaccg cccgatggtg gactatgtgc cgctgcaccg cgccaccagc
     1801 ggctcggaag acagccccat caagactgtg gtgcagtttg aaatgtccat cctcgactcg
     1861 ctgggcctgc tcaaggtgga ctttttgggc ctctcctcgc tgacggtgat gcagcgcgcc
     1921 tgcgcgctga ttgagcagcg ccacagccag cattttgacc tgggcaacat cccgctggat
     1981 gacccggccg cctttgaact gatgggcctg gggcatacgg cgggcgtctt ccagttggaa
     2041 ggctcgggca tgacccgctt cttgatgcag atgcaccccc aaaacctggc caacgtcatc
     2101 gccatggtgg cgctcttccg gccggggcca atggaattta tcccggctta catccggcgc
     2161 atgcacggcg aagagcgggt ggaatatctg gaccccgccc tcgagccgat ttttggcgag
     2221 acctttggca ttgccatcta ccaggaacaa ctcatgcgcg cctcggtgga cctggcgggc
     2281 tataccctgc ccgaatcaga tgacctgcgc aaggcgatct ctaaaaagca gaaagacaag
     2341 ctgctcaagc accgtgagaa attcatccac ggcgcctctg accggggcat gcccaccgaa
     2401 accgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagcca tgcggctgac
     2521 tacggcgtga ttgccgtgca aaccgcctac ctcaaggcgc actaccccga agaatacatg
     2581 accgccctga tctccgcctc caagagcgat tcggacaagg tggcgtttta tgtggccgac
     2641 tgccgcgcgc tggggattga tgtgctgccg ccggatatca acacaagctg ctgggatttc
     2701 accattgaag accgcccgga ccagcgcccc gccattcgct ttggcatggg cgcggtgaaa
     2761 aacgtgggcc aggccccggt ggagttgatt ttgcagggcc gcgccgatgg ccccttcaaa
     2821 gacctgaacg attttgccca ccgcgtggat ttgcgccagg tgggcaagcg ctcgctggaa
     2881 tgtctgacgc gcgtgggcgc gctggatgcc tttggtcagc gccgggcgct gctggatggg
     2941 ctcgaccgga ttattgcggt ctcgtccagc catttcaagg cgctgcaaag cggacagctc
     3001 agcttcttcg gtgccattac cggggtagaa gagcagattt cgctgccggc tgccggagag
     3061 gtggacaccc gcgagcagtt ggaatgggaa aaagaactgc tgggtctgta cgtctcggat
     3121 cacccgctct ccccgtatat gggggccatt cgccggcgcg tgacgcacac cgccagcacc
     3181 ctgggcgagg cccggcacaa ggcggtggtc acggtggcag gcatggtcac ccggctgcgc
     3241 ccgcatcaga ccaaggcggg caagcccatg ggctttgcca ccattgccga tctggaaggc
     3301 agcatcgaac tgatcctctt cccgcgcacc tgggccgaat ttggcaagct catccgcccg
     3361 gatgtggtga ttaccgtgca gggctcggtg gatgccgaag gcggcgaccc caaggtgctg
     3421 gtggaccggg tggaactgct gaacctggaa accctgcctg ccgaagcccc cgctgccgcc
     3481 ccggatgaga tcccctggga tgaggacggc ccgccgcccc cgcccgagcc agatgactgg
     3541 catttacagc cgcccccggc cgtagagccg ctctggatgg cgccccaggc cgcggatgtt
     3601 cagcctgccc cgccggcgaa cccgccggtt gagccgcccc cggcggcgaa aaccgtgccg
     3661 ccagcagccc aggccgagcc agccctcccg ccgcccccgg tggtgagcga acccgccccg
     3721 ccgccctacg agccgctgca ctacatcccc ccgcctgaag agagcaccgc caacccggca
     3781 gccaaccccc agcggatgat ccgcctggtg ctgcgcgcta ccggcgacaa acagcgcgat
     3841 ggccgccgga tgcgccggtt gtacgggatg ctggtctcct cgccgggccg cgataaattt
     3901 gcctttatga tctttgaaag cggccgccgc tatctgatgg aatttcccaa cgacagcacg
     3961 ggcatcacca acgcgctgat cgccgccctg ggcgagcaga ttggctcgga taatgtgatt
     4021 gtcgagacca tcccggtgca ttgatttttg agcctctgcc cggcacccgc gcgagatatg
     4081 atattaagca ggcagaaggg tgaagattgg aacaatgtca ttcaccccgg agcggcataa
     4141 aataataacg aacagcggcg cgtccatcgc agttcattgc agccagcact aaagctcatc
     4201 accgcgccgc tttttgcttt gaatctcccc acatctccca caagaggctt gccccatgaa
     4261 aaaccgctcc ctgctgatga ttccgggtcc cattgagttt gacccggctg tgcttgccgc
     4321 gctgggcgcg cccaccacca gccacctggc gcccgaattt atcgaagcct ttggtcaggc
     4381 actggagcgc acccgcgccc tcttccaatg ccccgacgga cagccatttg tgctggcggg
     4441 caccggcacc ctcggcatgg ataccgcctg cgccaatctg attgagccag gcgacgccgc
     4501 gctggtggtc aacaccggct attttggcga ccgcattggc gccattttgg aacgctacgg
     4561 cgcgcaggtc agccaggtgc gggccgcgcc gggcgggcac cccgcgctgg ccgcggtgga
     4621 gcaggccctg cagggcggcg gcttcaaggt gatgaccatc acccaggtgg atacctccac
     4681 cggtgtgctg gccgatgtgc gcggcctggc ggcgctgggt cagcgttacg gcgcgctggt
     4741 gatcgtggac ggcgtctgct cgctggcggg cgaagacctg aatatgggcg cctggggcgt
     4801 ggatgtggcc ttcacggcct cgcagaaggc ggttggcgtg ccgccgggac tggcgctgct
     4861 ggtggccggg ccgcgcgccc tggccgcgct gcaggcccgc cggacgcccg tgggcagcta
     4921 ctattccgac tggaacaact ggctgccggt gatgcaggcc tacgaagccc gcaaggcggc
     4981 ctattttggc accccggcgg tcaacctggt gtttgcgctc aacgtcagcc tggggcagat
     5041 tttgcaagag ggcctggcgg cgcgctttgc ccggcatgaa cgcatcagcc gggcgtgcaa
     5101 ggcgggcatt gccgccctgg ggctggggca ggtgccgctg aacgccgaca ttgccgccca
     5161 taccatgacg gccccacgct tcccggcggg cgtcagcgga gcagacctgc tgccccgggt
     5221 cgcccaggcg ggcgtcacgc tggcgggcgg gctgcatgcc gagatcaaga acgaatattt
     5281 ccgcattggg catatgggcg ccgtgacctc cggcgacgtg ctggcaaccc tcggcgccat
     5341 cgaaagcgcc ctggccgcct gcggccacaa atttcaaccg ggcgcggcgc tggccgctgc
     5401 ccagcaggtg tttttaggtt agggcgtcag tctgtcaatc aaaaacctcc ccgccagcaa
     5461 aggcggggag gtttattttt atgggagcgg cagctcaaag acccagggcc ccgggtaggt
     5521 gtagcccagc gcctctttgg ttagctgata gacctgctca tcgctcatgc ccggcgggcg
     5581 ctgaaggatg ttccaaccgc cgcccccgcc gccgttggcg ctgaaggtgt actgctcaat
     5641 ctgaatgccc tgcgcggcca gtttttcctg cgccgcggcc caggcatctg ccgggatgga
     5701 ttccggcatc gacagctcca ggccggggat gaccagcgtg agcacaccgc ccggctgctg
     5761 aacactgccg atggggaagc ccagttcgat gcagcggtcg gcgggctgcg agggggtgaa
     5821 gttccgctga ggatcgagga agagctgggc atcggcgtct tgcagcagcg cataattcaa
     5881 cagcgtggtc tgggtctggc tgccctccag ttgggtgccc gcgctgacgc cccaatcact
     5941 gccggggccg tgcgttggtt tgtcatagca gaggatcgcg ctggtgtaag aaggcgtgat
     6001 ttcggcccgc tccaggcgca gcgtcagccc gttggcggtc acagcctgca ctccggccag
     6061 ttggatgccc ggataaaccg gcaccgtcac ctggaactgg aaggccgcca gggtggtctg
     6121 ccagtcattg gcatcttgca cgggcaggtt gagcgtcagt tgggttgaat cgccgctcag
     6181 tttgtggctg gcatcaaaga tcagcaggta ggtgctgccg tcttcactgg atggctgccc
     6241 gatgttgaaa tccagggttt ttccgtcggc ggtggtcagg gttgcgtccc acagggtata
     6301 gctcggctgc cagccctcga agcgcatttg catggcaacc cgctgtgtat cggcatagac
     6361 ccaatcgagc gtggcggtca actgccccac ggtttgtgcg gcaatggggg tggctgtggg
     6421 ctgcggctgg atgacggtgt gcggggctga ttccggccag gccggggcct cggcaaaaaa
     6481 ttcccaggcg gttcccaccg tgggggtcag ggtcaggtgc agggtctcgg gctgctggct
     6541 ggcgggcagg tcaaagagca ggtcggcgca ggcaggggcc tgcggcaccg gcagaacctg
     6601 gtgcagccca gcgctgatgc gtggcggctc gcccaggccg cccaggtcag cctggatggt
     6661 caacgccgcc cagtcgggag cgggctcacc ggtggcacag acccgcgccc gggcggtctg
     6721 ggggcccagt actaccgcct ccagggtgcg ttcccgggca tcaatctggg cgctgtagat
     6781 gtactggctg gtcggcatct gccccggctg cacatccaga ccgttcagat ggaaggccgg
     6841 gccggtctgt ccggtcaggc ccagcagcag ggtcaggtcg gtgctgccag cgggcaggta
     6901 ttgattcagg cggtagatgc cggcccactg tccgccatcc tgctgcagcc acagcacctg
     6961 gccgcgcggc gcatcagtgg cgctgctgac ctgcacggca ggcagctgcg gttcagcggg
     7021 gctgccgctc atgctgaagc caaactgcag cccgctcgta ttgatttgca gccattccag
     7081 cgtccaggtg acgccctgaa gggtctgggt ttgacccaga atctgcacgg gggtttccgg
     7141 cagggccggc ggcgtggtga tctgtggctg cagggtgggg gcggcgcttt gctccaccgg
     7201 gctgaccagc ccggcttcct gcacggcatc aataccggga tcgtggaaga agacgcggta
     7261 aagggcatat ccggcgccgg tgattaatcc cagtccaagc aggacggcca gaaccgtcca
     7321 gacgattttc agcttcattt gttttttctc ctgcggttgg atgcgggcca ggatgcccgg
     7381 cagcagatta cgttcatcgg gaatatgctg ccgggcggct tcatccaaca gatttagaat
     7441 atggggaggt ttcatcgctc gctcctttcc agcagggcgc gcaggcgggc gcgggctgca
     7501 ttcagcagcc atttgaccgt gccaggggcg gcctgcaggt tctgggccat ttcctgttcg
     7561 ctcatttgca gataatagcg ctggacaatg gcagcccgct ggcgcggggc caggcgtttg
     7621 agggcctgca gcacctgctg ccgcagttcg gcggcttcga tcagactttc gggatcgggg
     7681 gtatctgcca gccactgctc aagatccatg ggagcgtcgg ggtcgatggg ccgcaatcgg
     7741 gcgttggact gggcctggtt gagcgccgca ttgacgacgc tgcgcatcag gtaggggcca
     7801 aaggggcggg tctcgtcaaa gcgccggatg tgatggtaga ggtgcaggaa aacgccctgc
     7861 accacatctt cagcctgggc ttcgtcctgc aggatgagaa acgcagcccg catggcgcgc
     7921 acctgatagc gcgttaccag atattccagt ccgctgatat cgccgcgttt gaggcgttga
     7981 atggcgtttt gttcttgctg caaagggtct tcgtttggag cgctcacaat ttatatacag
     8041 ttgacctgcc aaaaaggttg gggaattttt attataaccg gcgctttccg cgcgcggctt
     8101 ctgcgccagt gggtggctta gcacagacag gatcactcaa tccagccctc atcgaatttg
     8161 taggtaccgt agccttccca gtccaaccat tgactgttgt cttcgtctcg ggggaatggg
     8221 cggctaaaac agagcaagcc aatcccatta tcaggcaata cttcaatgcg atttgagtac
     8281 cagggaccac gtataaaaaa caaatccata tcaatcccgc ttt
//
LOCUS       DAOPVS010000085         8142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_8824_length_8142_cov_42.765550, whole genome shotgun sequence.
ACCESSION   DAOPVS010000085 DAOPVS010000000
VERSION     DAOPVS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8824_length_8142_cov_42.765550"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..4430)
                     /locus_tag="PKL11_07655"
     CDS             complement(195..4430)
                     /locus_tag="PKL11_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ13516.1"
                     /translation="MHNLVSHFILEKYIGKQNGGESGGLLHAVCLFADLSGFSIATHS
                     LLLHGQEAVESMAEVMLGIFTPLVSLVHAQGGFITTFAGDAFTALFPSEGDPASYFRA
                     LAAARAIQEHMRTHPKQDTLYGSFPFAIKLGLGDGEVEWGILESEQPAANPGAQNAAY
                     YFSGPAIEAAAGAEHQAQDGELILTQPVYAQLHTAIQGKALGVGHWCCLDVRDQLPAP
                     QPSRLDEALAAVEAAFIPPAISQRDSSGDFRQVVSVFIQLMGIETRQDLDIFMQAVFS
                     LLNQYEGYLNRVDFGDKGCNLLLYWGMPISHENDISRALDFVMELGNHTPGSFRAGLT
                     YRLMYAGLSGSTERGEYTCYGDGINLAARLMMEAPWGGIWVDEQVASRAQGHYEYESV
                     GLLTFKGFPEAQIVHNLLERRLTGQNYFDGQMVGRQAELTELNAFVQPLFAPAGERRY
                     AGILTVVGEAGLGKSRLVQEFISNESWPIAGAAQVFVCQTDQILRQSLNPFRRWLQNY
                     FDQSPSQSVERNKRAFSRKLDQLIAAQKNSELGQELNRTRSFLGALVGLEWESSLYAR
                     LDLQGRYENTLQSLQALLKAESLHQPVVLVLEDAHWLDEDSQEFVRRLEHAIEPYPIA
                     LLATARPERLDSLYGSQYPYRSLVLTNLSAEDLAALVRDRLGGPVAPQVLDLLVKRAE
                     SNPFFAEQILLYLREQNKLTFQNGVWSLVNSSHAALLPAEVRNIFIARLDCLSREIQN
                     IVHAAAILGREFEVRVLAHVMEIDHHGEGLPDELALQITTAEQESIWTALSQLRYLFR
                     HALLRDAAYEMQLKARRRELHRLAAVSLEHLSASDIRRGAVQMAEYVDQIAYHYETAY
                     CLGLEAVRPQAYQYLKQAARQAAERYENSASLDWYDRALALCPSEDRQACWDLTLGRE
                     AVLDRMARREAQFADLETLECLAKDLAAPDHQAQAAIRKANYYLATSDLQNAVTAAQM
                     AITFAQAAGQPLSQAEAHWHKGFALNRLGDFVGSRQHVEAALTLVRQSDDPLARRLMA
                     IALRLMAFLYENDPETMPQADSTLKESLHICREIGDKRNELRALNNLGVLALIKYFDL
                     AQAATYFKQAMDISQEIGDASARYYQFGNMEVVYEWQEDFAQAQAYGLQALHAAQEAG
                     DRFGEGDALSCLGNLARKTGDYGKSMALIQVGLDILQQVGEKSFLAGAQINLGWLYLE
                     LGNYDQATLHMEEVRTLANSMPNLAFDSAYMQGWLYLYQQDASGAQASFEQAMSNLGQ
                     DAPPNREADCWAGLGYAHMAARRPDMAAKAFENALVLRRRLNLPSQAFQAQIGLARAL
                     LDQGELTEAQAHTGDVMTFLEAEGDLTKGQGWGLAYLECVEILRVFDPAHSQQILQSA
                     RKILLARLATIPDEAQRKSFLEHIPWNRALMDA"
     gene            complement(4660..5982)
                     /locus_tag="PKL11_07660"
     CDS             complement(4660..5982)
                     /locus_tag="PKL11_07660"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ13517.1"
                     /translation="MKRNWTILITLVLIFPLLLTACAPEESNQLTASGFLSAVEASVA
                     PELSGRVLSISVEEGQTVTQGQPLFQLDDEYIIAQRDQASAAVQAAEAAVEAAQRQAD
                     SARAQYELALQGSLAQEMQTRQTAWAAPVLENFRPNWYFQKDELLSAAQNQVDAAIQE
                     LAANTADLDAELASTSSKDFVAAEERLAEAQLAYTIAQQTLTQVQSVNDTHLTDAATE
                     AMDATQAELDAALLDYQRMLTTTSAESVLEARARVSVSQIALDNANATLLGLQTGDKS
                     PQVKAAYAAVLAAESLVGQAEAGLAQAQQALNLAELQLDRTIIKAPLDGILLSRNLEV
                     GELAVAGGTVMQIAQIDTLDLIVYLPEDQYGRVMIGDTVLLKVDSYPDQQFSGEIIHI
                     SDEAEFTPRNVQTEQGRKATVYAVKITVLNPDHLLKPGMPADATFTLH"
     gene            complement(6006..7139)
                     /locus_tag="PKL11_07665"
     CDS             complement(6006..7139)
                     /locus_tag="PKL11_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13518.1"
                     /translation="MNRLWTIMRKELYHIWRDPRTLAMILALPALLLVLLGYGISGES
                     RDTTLAIVDYSKSDASRDYINRFTASDDFEVVYDVLSEAELINLIDRDLIDVGILIPE
                     DFDRKLMSGEQATAQIYIDGSMNPTDSLTVQLKLNAISSMAAQDVLVERVERAGLASG
                     LQLPITGILKTLYNPNGNNKLYMIPGLIPILLQVQTLLLSALAIVREREQGTMEQLIV
                     TPIRSWELMLGKTIPYLFVSAFNLFALLWLSKLLFGVTVAGSFWVLFGLSMVFVVGSL
                     GMGVLISTISQSQMQAIYVSVFIVLIPAIILSGLMFSRENMPAFTFWYSELLPVTQYL
                     EITRGIIVRGVGPETLWWSSTIPLLVLSVIYFAASIFIFRKRI"
     gene            complement(7136..8098)
                     /locus_tag="PKL11_07670"
     CDS             complement(7136..8098)
                     /locus_tag="PKL11_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13519.1"
                     /translation="MNAIEIRHLTRTFGDFAAIKDVSFTIPQGEIFGLLGPNGAGKTT
                     TIRILCGILLPTSGFAAVLGYDVTHKPEEVKSRIGYMSQKFSLYNDLTPVENINFYAS
                     IYGVPPKERRQRINELLQMADLVGHETQLTRNLSGAWRQRLALACAIVHRPQMLFLDE
                     ATAGVDPVSRREFWDLIYRLAGEGVSVLATTHYMDEADYCNTIGMMYQGELIALASPE
                     ALKEAQPGVLVQLDCDRPGVAADLASTQPGVLNTSVHGAQVHLTLKSKTYVTPVQKTL
                     TQAGIQIAGWEIIPPSLEDVFISMVAQRQTAAARPTESQNRSAA"
BASE COUNT         1844 a         2162 c         2361 g         1775 t
ORIGIN      
        1 gctacgcggt tgaaaatcag caatcagcgc tggaaaaatt caagcgccac aatcctgaga
       61 gcgaatttct acttgaaccc gcccaggata ttccttacga ggaagtggag cagcctgatt
      121 agaggttgat tgacgctgca tatacactgg gttgggaggg ggttactggt gaaatgtaaa
      181 acctctatcg aattttacgc gtccatcaat gcccggttcc agggaatgtg ttccagaaaa
      241 ctcttacgct gggcctcatc tggaatggta gccaaacggg cgagtaaaat ttttcgagcg
      301 gattgcaaga tctgttggga gtgggctggg tcaaaaactc tgaggatttc aacgcattcc
      361 aagtaagcca aaccccaacc ctgccccttg gtcaggtctc cctcggcctc caagaaagtc
      421 attacgtcac ctgtgtgtgc ttgcgcctcg gtcagttcgc cttgatccag cagcgccctg
      481 gccaagccaa tttgcgcttg aaatgcctgg ctgggcaagt ttagtctgcg cctcaagacc
      541 aatgcattct caaatgcttt tgccgccata tcagggcgcc ttgcagccat atgggcatac
      601 cccaggccgg cccagcaatc tgcttcacgg ttgggagggg catcttgccc aagattggac
      661 atggcctgct caaaagaggc ttgcgcccca gaggcatcct gctggtataa gtacagccaa
      721 ccttgcatat aagcactatc aaaagcgagg ttaggcattg aatttgccag ggtgcgcact
      781 tcttccatat ggagcgtcgc ctggtcgtaa ttccccagtt ccaggtacag ccagcccaga
      841 ttgatctggg cgcccgccag gaaggatttt tcacccactt gctgcaaaat gtcaagtccg
      901 acctggatca atgccatgga tttgccataa tcccctgttt tacgcgccag gttccccagg
      961 caactgagtg cgtccccttc gccaaatcgg tcgccggcct cttgggccgc atgcaatgcc
     1021 tgtaaaccat acgcttgcgc ctgtgcaaaa tcttcctgcc attcgtacac aacttccata
     1081 tttccaaatt ggtaatacct ggcagatgca tcgccaattt cttgggagat atccatcgct
     1141 tgcttgaaat acgttgcggc ctgtgcaagg tcaaaatatt taatcagggc aagcacccct
     1201 aaattattaa gcgctcttaa ttcgttccgt ttatcgccaa tttcccgaca gatatgtaag
     1261 ctttccttca aggtactatc tgcctgcggc atggtctcag ggtcattttc gtacaagaag
     1321 gccatcaggc gcagtgcaat cgccatcaag cggcgggcaa gtgggtcatc cgattgcctg
     1381 accagggtca gggcggcttc aacgtgctgc cggctgccca caaaatcccc caagcgattc
     1441 agggcaaagc ccttgtgcca gtgcgcttct gcctgactga gcggttggcc ggctgcctgg
     1501 gcaaaggtaa tcgccatctg ggctgcagtg acagcatttt gcagatcgct ggtggccagg
     1561 taataattcg ctttgcgtat tgccgcctgc gcctggtggt ctggagcagc cagatccttc
     1621 gccaggcatt ccagggtttc cagatctgcg aactgcgcct cacggcgtgc catcctgtcc
     1681 aaaaccgctt cgcgtcccag ggtcagatcc cagcatgcct gccgatcttc tgatggacac
     1741 agcgccagcg cccggtcata ccaatccagg ctggccgagt tttcataacg ctcggcggcc
     1801 tggcgcgcgg cttgcttcag gtattgatat gcttgagggc gcaccgcttc cagaccgagg
     1861 caataggccg tttcgtaatg gtaggcaatc tgatctacgt actcggccat ttggacagca
     1921 ccgcggcgaa tatctgacgc gctcaaatgc tccagtgaga cagccgccag gcggtgcagt
     1981 tctcgccgcc gggctttgag ctgcatttca tacgctgcat ctcgcaaaag tgcgtgcctg
     2041 aagagataac gcagctggct taaggcggtc caaatagatt cctgttcggc tgttgtaatc
     2101 tgtagtgcca gttcgtctgg taacccttca ccgtgatggt caatttccat cacatgggcc
     2161 aatacgcgga cttcaaattc tcgtcccagg attgccgcgg cgtgcacaat attctggatt
     2221 tctctggaca ggcaatccag gcgggcgatg aagatgttgc gcacctcagc cggtaagagg
     2281 gctgcgtggc tggaattaac cagagaccag actccgttct ggaaggtcaa tttattttgt
     2341 tcgcgcaggt agaggagtat ttgctcggca aagaaggggt tgctttccgc ccgcttgacc
     2401 aggagatcca aaacctgcgg ggcaactggc ccgcccaggc gatctcgcac cagcgccgcc
     2461 agatcttcag cagaaaggtt ggttaatact aatgaacggt aggggtattg agatccatag
     2521 agcgagtcca gccgttcagg acgggcggta gccagcagcg ctattggata aggctcgatg
     2581 gcgtgctcta gccgccggac aaattcctga gagtcttcat ccagccaatg tgcatcttcg
     2641 agcaccagta ccaccggttg atggagactt tcggccttga gcagcgcctg gagcgactgg
     2701 agcgtattct cgtaacgtcc ctgtagatca agccgggcat agagtgagct ttcccattcc
     2761 aaccccacca gcgcccctaa gaaggagcgc gtacgattca gttcctggcc cagttccgaa
     2821 ttcttctggg cggcaatcaa ttggtcgagc ttgcggctga aggcgcgttt gttgcgctcc
     2881 acggattgag aaggggattg atcgaagtaa ttctgcaacc agcgacggaa gggattgagg
     2941 gattggcgca agatctggtc ggtctggcag acaaaaacct gggcggcccc ggcaataggc
     3001 caactctcgt tggagataaa ttcttgtacc aggcggctct tgcccaaccc agcctcaccg
     3061 accaccgtca gaatgccagc atagcgccgt tcgcccgccg gggcaaaaag tggctggaca
     3121 aaggcattca gttcggttag ttcagcctgg cggcccacca tttggccatc gaagtagttt
     3181 tggcccgtca gacgacgttc gagtagatta tggacgatct gtgcttcggg aaaacctttg
     3241 aaggtcagga gacctacgga ttcgtattca taatgtccct gggcacggct ggcgacttgt
     3301 tcatccaccc atatgccgcc ccaaggagct tccatcatta agcgcgctgc caggttgatt
     3361 ccatccccat agcaggtgta ttcccctcgc tcggtggaac ctgaaagccc tgcgtacatg
     3421 aggcggtagg tcagccccgc gcggaaagag cccggggtgt ggttgcccag ctccatcaca
     3481 aagtccaggg cgcgggagat gtcattctca tggctgatcg gcatacccca gtacagcagc
     3541 aggttgcagc ccttgtcgcc aaaatcgacc cggttaaggt atccctcgta ttggttgagc
     3601 aggctgaaga ccgcctgcat gaagatatcc aggtcttgcc gggtttcgat acccatcagt
     3661 tggataaaga cagaaaccac ctggcgaaaa tccccgctgc tatcgcgctg gctgatggcc
     3721 ggggggatga aagccgcctc aaccgctgcc agggcttcgt caagcctgga gggctgagga
     3781 gcaggcagtt ggtcccgaac gtccaggcag caccagtgcc ctacgcccag tgccttgccc
     3841 tggatggcag tatggagttg agcgtagact ggctgggtaa ggatcaactc tccatcctgg
     3901 gcctggtgtt cggcgccggc cgccgcttca atggcagggc cactgaagta ataagccgcg
     3961 ttctgcgccc cgggattagc ggcaggctgc tcgctttcca ggatgcccca ttccacctca
     4021 ccatcgccca ggcccaattt gatagcaaag gggaagcttc catagagcgt gtcttgtttc
     4081 gggtgggtgc gcatgtgttc ctggatagcc cgtgcagccg ccagcgcccg aaagtagctg
     4141 gcagggtccc cttccgaagg aaagagtgcc gtgaaagcgt ctccggcgaa ggtggtgatg
     4201 aaacctccct gggcatgtac cagcgaaacc agaggggtga aaattcccaa catcacctcc
     4261 gccatggatt ccaccgcttc ttgtccgtgc agcagcagtg agtgggtggc tatgctgaag
     4321 ccagagaggt ctgcaaacag gcacaccgca tgcagcagcc caccactctc accgccgttc
     4381 tgtttgccaa tgtacttttc gagtatgaag tgagaaacaa gattgtgcat caatttgtcc
     4441 tggttatccc catggtatta gataggaaat agccacccaa ttgtaccaga gtattaatct
     4501 gatagacttg cgccgcggag tctaacacag agaaagctct gggcaggccg tataaacaat
     4561 gaagtctcat caagcaggtc attgacatgg atgtcgatat ggcgaaaaaa ataaaagccg
     4621 catcatgcaa agatgcggct caagcagaag acagatgggt tagtgcaatg tgaaggtggc
     4681 gtcggcgggc atgccgggtt tgagcagatg atcggggttg aggacggtaa ttttgacggc
     4741 gtagacagtg gccttgcgcc cctgctcggt ttggacgttg cggggggtga attcggcttc
     4801 atcggagatg tggatgattt caccgctgaa ctgctggtca gggtaggagt ctactttgag
     4861 caggacggtg tcgccaatca tgacgcggcc atactggtct tcgggcagat agacaatcag
     4921 gtcgagggtg tcgatctggg cgatttgcat caccgtgccg ccagccacag ccagctcgcc
     4981 gacctcgagg ttgcgggaga gtaagatgcc atcgaggggg gctttgatga tggtgcgatc
     5041 cagttgaagc tctgccaggt tgagggcctg ctgggcctgg gctagacctg cctcagcctg
     5101 ccccaccagg ctttcggcgg ccagcactgc ggcataggcg gccttgacct ggggagattt
     5161 atcgccggtt tgcaggccaa gcagggtggc gttggcgtta tccagggcaa tctgtgagac
     5221 gctgacccga gcacgggctt ctaacacaga ttcagcagag gtggtggtca acatgcgctg
     5281 atagtccagc agggcggcat ccaattcggc ctgggtagca tccatggctt cggtggcggc
     5341 atctgtcagg tgagtgtcat tgaccgattg aacctgggtg agagtttgct gggcaatggt
     5401 gtaggctaac tgtgcctcag ccaggcgctc ttcggcagcc acaaaatctt tgctgctggt
     5461 gctcgccagc tcggcgtcca gatcggcagt gttggcggca agttcctgga tggcggcatc
     5521 cacctggttt tgagccgcgg acaggagttc atccttttgg aagtaccagt tggggcggaa
     5581 gttttcaagc acgggggcgg cccaggcggt ctggcgggtt tgcatttctt gggccaatga
     5641 accctgaagc gccagttcat actgggcccg ggcactgtcg gcctgacgct gggcggcttc
     5701 aactgcggct tcggcagcct ggacagcggc agaggcctga tcacgctggg caatgatata
     5761 ttcatcgtcc agttggaaga gcggctgccc ctgagtaact gtttggccct cttcaacgga
     5821 gatggacaga acccgcccac tcaattcagg ggcgacagag gcttctacgg cagatagaaa
     5881 acccgaagcc gtgagctgat tgctctcttc cggcgcgcag gcggtgagca agagcgggaa
     5941 aatcaaaacc agggtgataa gaatggtcca attgcgtttc atgatcgtct ccttctcaat
     6001 tgaagttaga tacgtttgcg gaagatgaag atgctggcgg caaagtagat gacactgagc
     6061 accaacaggg gaatggtgga tgaccaccag agggtttcgg ggcccacgcc ccgaacaatg
     6121 atgccgcgcg taatttccaa atactgggtt acaggcaata attcgctgta ccagaaggtg
     6181 aaagcgggca tgttctcgcg tgagaacatc agccctgaca ggatgatggc agggatcagt
     6241 acaatgaaca ctgaaacgta gatagcctgc atctgagatt gcgagatggt tgaaattagc
     6301 acccccatgc ccaatgagcc gaccacaaag accatgctga ggccaaagag cacccaaaaa
     6361 ctgccggcaa cggtaacacc aaagagcagt ttactcagcc agagcaaggc aaacaagttg
     6421 aaagcactga caaacaggta ggggatggtt ttgcccagca ttaactccca ggaacggatg
     6481 ggggtgacga tgagctgctc catggtgccc tgctcacgct cgcgcacaat ggcaagggcc
     6541 gaaagcaaaa gggtttgaac ctgcagcaag atggggatga gccccgggat catgtacagt
     6601 ttgttgttgc cattgggatt gtagagggtc ttgagaatgc cggtgatggg taactgcaac
     6661 ccagaagcca ggcctgcgcg ctcaacccgc tcaaccagga catcctgcgc ggccatggaa
     6721 ctgatggcgt tgagttttag ctgcaccgtg agcgaatcgg ttggattcat ggagccgtca
     6781 atgtaaattt gggcggtggc ctgttcgcca gacatgagct tgcggtcaaa atcttcaggg
     6841 ataaggatgc cgacatcaat caggtcacgg tcaatcaaat taatcaactc agcttcactc
     6901 aacacgtcat aaacaacctc aaagtcatca ctggcggtga agcggttgat gtagtcgcgg
     6961 ctggcatctg atttggaata atccacgatt gccagggtgg tatcgcggct ttcgccggaa
     7021 atgccgtagc ccagcaagac cagcagcagg gctggcaagg ccaaaatcat ggccagcgta
     7081 cgggggtcac gccagatgtg gtacaactct ttgcgcatga ttgtccataa tcggttcatg
     7141 ctgcgctcct gttttgagat tcggttggcc gcgcagcagc ggtttggcgc tgcgccacca
     7201 tggagatgaa tacatcctca agtgaggggg ggatgatttc ccaacctgcg atttgaatgc
     7261 ccgcctgggt cagggttttc tgaacagggg ttacatatgt tttcgacttg agagtcaggt
     7321 gaacctgggc gccgtgcacg gatgtgttca ggacacccgg ctgggtgctg gcaagatcgg
     7381 cagcaacccc cgggcggtca caatccaact gcaccagcac gccgggctgc gcttctttca
     7441 gagcttcggg gctggccaga gcaatcaatt ccccctgata catcatgcca atggtattgc
     7501 agtaatcggc ttcgtccata taatgcgtgg ttgccagcac gctgacgcct tcaccggcca
     7561 ggcgatagat cagatcccag aattcacggc gagaaacagg atcaacaccg gcggtggctt
     7621 catcgaggaa cagcatttgc gggcggtgaa caatggcaca ggccagggcc aaacgctggc
     7681 gccaggcgcc cgaaaggttg cgggtaagct gcgtctcatg cccaaccagg tcggccatct
     7741 ggagaagctc attgattcgc tggcggcgtt ctttgggcgg cacgccgtaa atggaagcat
     7801 aaaagttgat attttcaact ggggtcaaat cgttatacag cgaaaatttt tgagacatgt
     7861 agccaattcg agatttgacc tcttcaggtt tgtgagtcac atcgtagccc aggaccgcag
     7921 caaaaccact ggtggggagc aaaatgccgc acagaatccg gatggtggtg gtcttgccgg
     7981 cgccattggg gccaagcaaa ccaaagatct caccctgggg gatggtgaag ctgacgtctt
     8041 tgatggcggc gaagtcgcca aaggtgcggg tgagatggcg aatttcaatg gcgttcatga
     8101 gcgacctcgt tcaatattga ccaggtggac aaaaacatct tc
//
LOCUS       DAOPVS010000086         8063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_8934_length_8063_cov_55.882493, whole genome shotgun sequence.
ACCESSION   DAOPVS010000086 DAOPVS010000000
VERSION     DAOPVS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8934_length_8063_cov_55.882493"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1332)
                     /gene="serC"
                     /locus_tag="PKL11_07675"
     CDS             complement(235..1332)
                     /gene="serC"
                     /locus_tag="PKL11_07675"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNZ13520.1"
                     /translation="MTPVKRVHNFNAGPSVLPVSVLEKAQAEILDFQGCGMSVMEMSH
                     RSKEFEGIIQTAEADLRELMNIPANYKVLFLQGGASLQFAMIPMNLRPAGASADYIVT
                     GAWSRTAAKEAAKLGATHTAFTSEADNFSRLPADNEIQLDPNAAYVHFCHNETIHGVE
                     FKTEPRVPAGVPLICDMSSDFLSQPVDVSPYAMIYAGAQKNAGPAGVVVAIIREDLLA
                     RVPEKLPVMLDYKIMVENGSLYNTPPCWSIYIVGLVMKWLKELGGLPAIHAINQQKAN
                     LIYGAIDQSGGYYRGHAAPAARSIMNVPFRMPTEELEELFVKEAKKAGFIGLKGHRSV
                     GGLRASIYNALPIESAKALVEFMAEFQRKNG"
     gene            1583..4165
                     /locus_tag="PKL11_07680"
     CDS             1583..4165
                     /locus_tag="PKL11_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNZ13521.1"
                     /translation="MQHLLEKWFLKPAPEMAGADPAAAPAGWMFSQNSGGSKPRVVRG
                     ERRRPTAPSSTPRPRAEAPQRQSAPRPSTSGGGFTPPTFSGGSSGGTARPMGFNLPGG
                     KRTPIILIIIVAVIAICLCLALSQGGGGLGDILSQLGGGEYTDYTVTEDAGNNYTYTE
                     APTLPTRTPRPMTASGDTWLVLLYQDADDQVLEKDIYFDLNEAERVGSSEQVTILAQM
                     DRFRAGYQGDGNWTSARRYYVTQDNDLTRVSSEMIADLGEVNMADSQTLIDFVTWGIE
                     NYPADKVVLILSDHGMGWPGGWTDPTASGAPDANSMPMAAQLEDHLYLMEIDEALGQI
                     RSQTGLDQFEMIGMDACLMGHVEVLSAMEPHARYFVGSQEVEPSLGWAYAAFLDALTA
                     NPGMDGGELGQIIVDSYIVEDQRIMDDSARADFASGASPMNSFFGGYSVPSASQITAQ
                     LERDITLTAVDLSAVPNLINSLNNFAYAMQDADARAIAQARTYAQSFTNIFGNGTQPS
                     YIDLGNFAQMVRDSSSNSAVNAAASDLLTAINQAVVAEHHGSKRPGATGISIYFPSVQ
                     LYRTGVAGPQSYTVAARRFAEESLWDEFLVYHYTGKNFEAAQNIVATPRTSDIRGAVA
                     GGVSVGQLQLSSTSIGLGESILMSLDIAGENVGYIKLLLGYYEPTSNSILMMDSDYIA
                     SAETRSVDGVYYPDWGEGDFTLEFDWEPIVYAISDGQTTAVALFTPEVYGANSEEAIY
                     SVDGKYTFADSGEEISAKLYFRNGLLWQVYGFSGSGEASAPREITPQAGDTFTVRYQW
                     LELDAQGSVSQVVNEPGETLIFGNEAFQWVTLDAAAGQYLLGFIVEDLNGTQTPVMGQ
                     ITVR"
     gene            4262..4828
                     /locus_tag="PKL11_07685"
     CDS             4262..4828
                     /locus_tag="PKL11_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ13522.1"
                     /translation="MDNRDHLLLCALRLFATRGYEAVGVQEIVEAAGVTKPTLYHYFG
                     SKRGLLEALLSENFEALNRALRPAAAYSGDLPLTLKQIAAAYFDFANAHPAFYRFQLA
                     LWFAPGNSEENQVVTRFNRQQYELVEQVFAQAVAQHGNMRGRQRAYATTFIGMVNTYI
                     GMALNGYVQLDVVLLEQALHQFQHGIYS"
     gene            4894..6252
                     /locus_tag="PKL11_07690"
     CDS             4894..6252
                     /locus_tag="PKL11_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ13523.1"
                     /translation="MTHWIDTAVFYHIYPLGLCGAPQQNDHQAPPQPRLLELIPWLDH
                     MITLGCNALYLGPVFESSAHGYDTADYYWVDRRLGDHDTLRHLVAEAHRRNIRVVLDG
                     VFNHVGRHFWAFRDVQQHRQNSAYCSWFAGLRFDQSNPYGDGFNYDTWAGFYDLVKLN
                     LHHPDVRRHLLDAVGQWIDLYEIDGLRLDAADVLDRDFQRELAGFCRARRADFWLMGE
                     VVHGDYRQWANPGALDSVTNYEAYKGLFSSLNDRNYFEIAYALQRQFGAGGLYTGLPL
                     YNFVDNHDVDRVVSRLKQPAQLYPLYALLFTMPGVPSIYYGSEFGLTGKKNGSDAPLR
                     PALDLMQIKGNAPQPDLPTAIQRLAQVRQVLPALRVGCYEQVYVGPAQLVFRRQTEHQ
                     TVIVALNADAAPVQLEVRLTGLTDGWLKDALNPPDQIRVQYSRAVLELPANWARVMVW
                     EA"
     gene            complement(6256..7764)
                     /locus_tag="PKL11_07695"
     CDS             complement(6256..7764)
                     /locus_tag="PKL11_07695"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNZ13524.1"
                     /translation="MNKTLGLLSGFILLVMLLSACRTPAPVLTSTPPALETPQFAEGR
                     EIYRSGLIESAQPILAELPGATEYRLDITIAADYLSVQGHESIRYTNRESDSLDAIYL
                     RLFPNIAGGDLTVSNVTIAAQPVQPVEEAYRSSLRLPLAAPLAPQDAVELELDFALTL
                     PTEMGGNYGLLGFFDDILVLDTFYPVIPVYDAQGWNVSVPPPNGDLPYFDVSFYQVRV
                     TAPANLVMAASGLAVDQKVNADWQTVTYAVGPARDFYLAASEQFVRTTQVVNGVTVNC
                     YAFENRSEASAQALQVVAAALEHYGARFGPYPYTEFDVVSTPMLALGVEYPAITAINM
                     ELFDPATIIRGIPAHVWLESVTAHEMAHQWFYNLVGNDQADEPWLDEAAAQYCTYLYY
                     LDEYGLAAADEYRGEWVARWARVDQAEIPIGLPSGDYAAEEYSPIVYGRGPIFLFNLA
                     ETMGEDRFDAFLSAYADQYQWGIATTADFKALAEQSCDCDLTPLFTDWIYGP"
BASE COUNT         1795 a         2049 c         2480 g         1739 t
ORIGIN      
        1 tcatcaggtg cgggtcactt tataaaggta aatgtcagac aatttaaccg gatgcgacag
       61 aggtattata ctccaggcat ttgcaattca aagattagga aaaccataga gtctttaggc
      121 gttagggctg agaaacatct atgctgcctg ctaaccatta gtcaaccaga attgaaataa
      181 aaagcccgcc gccggttctg ccaggcggcg ggtttatggg gttatggaag cgaattagcc
      241 gttcttgcgc tggaattcgg ccatgaattc gaccagggct ttggcggatt cgatgggcag
      301 ggcgttgtag atggaggcac gcaggccgcc aaccgagcgg tgacccttga ggccaatgaa
      361 gccggctttc ttggcttctt tgacaaagag ttcttcgagt tcttcggtgg gcatgcggaa
      421 gggcacgttc atgattgagc gggctgccgg agcggcatgg ccgcggtagt aaccgccgct
      481 ctgatcgatg gcgccgtaga tgagattggc tttctgctga ttgatggcat ggatggccgg
      541 gagaccgccg agttctttga gccatttcat caccaggcca acgatgtaga tggaccagca
      601 cggcggggtg ttgtagagcg agccattttc gaccatgatc ttgtagtcga gcatgacggg
      661 cagtttttcg ggcacgcggg ccagcaggtc ttcgcggatg atggcaacaa ccacaccggc
      721 agggccagca ttcttctgag cgccagcata gatcatggcg tagggggaga catccacagg
      781 ctggctgagg aagtctgagg acatgtcgca gatgagcggc acaccggcag gaacgcgggg
      841 ttcggtcttg aattctacgc cgtggatggt ttcattatgg cagaagtgaa cgtaggcagc
      901 gttagggtca agctgaattt cattgtcggc gggcaggcga gagaagttgt cggcttcgct
      961 ggtaaaggcg gtatgcgtag cgccgagttt ggcggcttct ttggcggctg tgcggctcca
     1021 ggcgccggtg acgatgtaat ccgctgatgc accggcaggg cgcaggttca tggggatcat
     1081 ggcaaactgg aggctggcgc cgccctgcag gaacaggact ttgtaattgg cggggatatt
     1141 catcaactcg cgcaaatcgg cttctgccgt ctggatgatg ccttcaaatt ccttggaacg
     1201 atggctcatt tccatcacag acatgccgca gccctggaaa tcaaggattt cggcctgggc
     1261 tttttcgaga acagagaccg gcagcacgga ggggccggcg ttgaaattat ggacacgctt
     1321 tacgggggtc attttttctg cgcctcttct gaatgttaat ggtggatggg caaggttggg
     1381 ttagccccat tgatccctca cccgttgata ttaacactat cataccgcaa gaaagcaaag
     1441 tttgcatatg tcatttgtct tgaagtgtca aaacaattgc catacaggag gaaattaaac
     1501 gtataataag tatattcggg caggtgattc aggctttgaa tcgctgccca agttcaactg
     1561 gaaattggag ggaggacgtc ccatgcaaca tttacttgaa aaatggtttt taaagccggc
     1621 cccggaaatg gcaggcgcag acccggctgc agcacccgca ggttggatgt tcagccagaa
     1681 cagcggcggg agcaagccgc gggtggtgcg cggcgaacgc cgccgcccga ccgcgcccag
     1741 cagcacccca cgtccacggg cagaagcacc gcagcgccaa tcggcgccgc gccccagcac
     1801 cagcggcgga ggcttcacac cgccgacatt cagcgggggc agcagcggcg gaaccgcccg
     1861 cccaatggga ttcaacctgc cgggcggcaa gcgcacgccg atcattttga tcatcatcgt
     1921 ggctgtgatt gccatctgcc tgtgcctggc gctatctcag ggcggcggcg ggctgggtga
     1981 tatcctttcg caattaggtg ggggtgagta cacagattac accgtgacgg aggatgctgg
     2041 taataattac acctataccg aagcgccaac cctccccacc cggaccccgc gcccaatgac
     2101 tgcctctggt gatacctggc tggtgctgct ctatcaggat gccgacgacc aggtgctgga
     2161 aaaagatatc tactttgatt tgaacgaggc cgaacgcgtt ggctcgagcg agcaggtcac
     2221 catcctggct cagatggacc gcttccgggc cgggtatcag ggcgacggca actggaccag
     2281 tgcgcggcgc tattatgtga cgcaggataa cgacttgacc cgtgtcagtt cagaaatgat
     2341 tgctgacctg ggtgaagtga atatggcgga ttcacagacg ctgattgact ttgtgacctg
     2401 gggcattgaa aattacccgg ctgacaaagt ggtcttgatc ctctcagacc atgggatggg
     2461 ctggccgggc ggctggaccg acccgactgc cagcggcgcg cccgatgcca acagcatgcc
     2521 aatggcggct cagttggaag atcacctgta cctgatggaa attgacgaag ccctggggca
     2581 aatccgatcg caaaccgggc tggatcagtt tgaaatgatc ggcatggacg cctgtctgat
     2641 ggggcatgtg gaagtgctga gcgccatgga acctcacgcc cgctactttg tgggttcgca
     2701 agaggttgag ccctcgctgg gctgggccta tgcagccttc ctggatgcgc tgacggccaa
     2761 ccccggcatg gatggcggcg aactggggca gatcattgta gacagctata ttgttgaaga
     2821 tcaacggatt atggacgaca gcgcccgggc tgattttgcc tcgggggctt ctccgatgaa
     2881 cagtttcttt ggcgggtaca gtgtgccctc cgccagccag attacggcgc agttggaacg
     2941 ggacatcacc ctgacggcgg tggatttgag cgcagtaccc aacctgatca acagtttgaa
     3001 taattttgcg tatgccatgc aggatgccga tgcgcgggcg attgcccagg ctcgcaccta
     3061 tgcgcagtcc tttaccaata tttttggcaa cggcacgcag ccctcttaca ttgacctggg
     3121 gaactttgcc cagatggtgc gagacagcag ctcgaacagc gcggtgaatg ccgcagcaag
     3181 tgacctgctg accgccatca atcaggcagt ggtggctgaa caccacggat ccaagcgccc
     3241 cggagccacc gggatttcga tctacttccc gagtgtgcag ttgtaccgca ccggcgtggc
     3301 ggggccgcag tcttacacag tggcggcacg gcgctttgca gaagaatcgc tgtgggatga
     3361 gtttttggtc tatcactata ccggcaagaa ttttgaggcc gcccaaaaca tcgttgccac
     3421 accgcgcacc agcgatatcc gcggggctgt ggcaggcggc gtatcggtgg gacagttaca
     3481 gctctccagt acatcaattg ggttgggcga atcaatcttg atgagcctgg acattgccgg
     3541 agagaatgtg ggctatatca agctgctgct ggggtattac gagcccacca gcaactccat
     3601 tttgatgatg gacagcgatt acatcgccag cgctgagacg cgcagcgtgg atggcgtgta
     3661 ctacccggac tggggggaag gcgactttac gctggagttt gactgggagc cgattgtgta
     3721 tgccatctcc gatgggcaaa ccacggcggt ggcgctcttt acgccagaag tttacggcgc
     3781 caattcagaa gaggcaatct acagtgtgga tgggaagtac acctttgcag attcgggtga
     3841 agaaatcagc gccaagctgt acttccgcaa cgggttgctg tggcaggtgt acggcttcag
     3901 cggcagcggc gaagccagcg ccccacgcga aattaccccg caggctggcg atactttcac
     3961 cgtccgatac cagtggttgg aactggatgc gcaagggtcg gtaagccagg tggtgaatga
     4021 acctggcgaa acactgatct ttggcaatga agccttccag tgggtaacgc tggatgctgc
     4081 tgcgggtcag tacctgctgg gattcattgt agaagacttg aatgggaccc aaacccccgt
     4141 catggggcag attactgtcc ggtaaacgca gttcaacaac gttcgatgcg gggcgcaagg
     4201 aaaccttgcg ccccgttttg tatcttgaca gggactatac cgatcggtat aatgagccgt
     4261 aatggataat cgtgatcatc tgctgctctg tgcgctgcgc ctgtttgcca cccgcgggta
     4321 tgaagccgtg ggggtgcagg aaattgtgga agcagccgga gtgaccaaac ccactctcta
     4381 tcactacttt ggcagcaagc gcgggctgct ggaagcgctg ctgagcgaaa attttgaggc
     4441 gctcaaccgg gcactgcgcc cggcagcggc ttattcgggc gacctgcccc taaccttgaa
     4501 gcagattgcc gccgcttatt ttgactttgc caatgcgcat ccggcgtttt accgctttca
     4561 actggcgctg tggtttgcgc ccggcaatag cgaggaaaat caggtggtta cccggttcaa
     4621 ccggcagcaa tatgagctgg tggagcaggt ttttgcgcag gcagtagccc agcacggcaa
     4681 tatgcgcggg cggcagcggg cctatgccac cacattcatc gggatggtga atacctatat
     4741 cggcatggcg ctcaatggct atgtacagtt ggatgtggtt ctgttggagc aggcgctgca
     4801 tcagttccag catggaatct attcctgagg ggaatcctct cttttttagc ccgattctat
     4861 accgatcggt atattctttt acggagaaac gctatgactc actggattga cacggctgtt
     4921 ttctatcata tttacccact gggactgtgc ggcgcgccgc agcaaaatga tcaccaggcg
     4981 ccgccccagc cgcgcttgct ggaattgatt ccctggctgg accatatgat cacgctgggc
     5041 tgcaatgcgc tgtacctggg tccggtgttt gaatccagcg cgcatggcta cgacacggct
     5101 gattattact gggtagatcg gcggctggga gatcacgaca cactgcggca cctggtagcc
     5161 gaagcgcacc gaagaaatat ccgggttgtg ctggacgggg tgtttaatca tgtggggcgg
     5221 catttctggg cctttcgaga tgtgcagcaa caccgccaga attcggctta ctgcagttgg
     5281 tttgccgggc tgcgatttga ccagtccaac ccatatgggg atgggtttaa ttacgacacc
     5341 tgggcgggat tttatgatct ggtcaaatta aatttgcatc acccggatgt gcggcggcac
     5401 ctgttggatg cagttgggca gtggattgat ctgtatgaaa tcgatggcct gcggttggat
     5461 gcggctgatg tgctggatcg agattttcaa cgtgagctgg cggggttctg ccgggcgcgc
     5521 cgggcagatt tctggttgat gggcgaagtg gttcacggcg attaccggca gtgggctaac
     5581 ccgggggcac tggactcggt gaccaattat gaggcgtata aggggttgtt ctccagcctg
     5641 aatgaccgca attatttcga aattgcctat gcgctgcagc ggcagttcgg cgcgggtggt
     5701 ttgtatacgg gcctgccgct ctataatttt gtggataatc acgatgtcga tcgggtagtc
     5761 agtcgattaa aacaacccgc tcaactttat ccgctgtatg cgctcctgtt caccatgccg
     5821 ggggtgcctt ctatttacta tgggagcgaa tttggcctga ccgggaaaaa gaatggcagc
     5881 gatgcaccgc tgcgtccggc gctcgatttg atgcagatca aggggaatgc gccgcagcca
     5941 gatttaccga ccgccattca aaggctggcg caggtacggc aggtgctgcc cgccttgcgc
     6001 gtggggtgct atgaacaagt ctacgtcggc ccggcgcaac tggttttccg gcggcagacc
     6061 gagcatcaga ctgtgattgt ggcgctcaat gccgatgcgg ctccagttca gttagaggtg
     6121 cggctgacgg ggctgacaga cggctggctg aaggatgcgc taaacccacc agaccagatc
     6181 cgagtgcagt acagccgggc tgttttagaa ttgccagcca actgggcgcg ggtgatggtt
     6241 tgggaggcgt gactttcagg ggccatagat ccagtcagta aacagcggcg tgaggtcaca
     6301 atcacaggat tgttcagcca gggctttgaa atccgcggtg gtcgcaatgc cccactgata
     6361 ttgatcggca taggctgaca gaaaggcatc aaagcgatct tcgcccatgg tttcggccag
     6421 attgaacagg aagatagggc cgcgtccata gacaatgggg ctgtattcct cagcagcata
     6481 atcgccggaa ggcaagccga tggggatttc ggcctggtca acccgcgccc accgggcaac
     6541 ccattcgccg cggtattcat cggcagcagc cagcccatat tcatccagat aatacaggta
     6601 ggtgcagtat tgggccgcgg cttcgtccag ccagggctcg tctgcctggt cattgccgac
     6661 taaattatag aaccattgat gcgccatttc gtgggcagtg acagattcca gccagacatg
     6721 ggccgggatt ccgcgaatga tggttgcagg gtcaaataat tccatgttga tggccgtgat
     6781 ggcggggtat tctaccccca gcgccagcat gggcgtgctg acgacatcaa attcggtgta
     6841 ggggtaaggg ccaaagcggg caccatagtg ctccaaagca gccgcaacaa cctgaagcgc
     6901 ttgagcagat gcttcggagc ggttctcaaa agcgtagcag ttcaccgtta cgccattgac
     6961 cacctgcgtg gtgcgcacaa actgctcgct ggcagccaga taaaagtctc gggcggggcc
     7021 aacggcatag gttacagtct gccaatcggc attcactttc tgatctacgg ccaggccgct
     7081 ggcagccatc accagattgg ccggagcggt cacgcgcacc tgatagaagc tgacatcaaa
     7141 atagggcagg tcgccattgg gcgggggaac gctgacattc cagccctgcg catcatagac
     7201 cgggatgacc ggataaaatg tatccagcac caggatatcg tcaaagaagc cgagcaggcc
     7261 ataattgccg cccatttctg tgggcaaagt gagggcaaaa tccaactcta attccactgc
     7321 atcttgaggg gcaagcggag ccgccagggg caggcgcagc gaactgcgat aggcttcttc
     7381 aacaggttga actggctgag ccgcaatggt cacgttggaa acggtcagat cgccgccagc
     7441 gatgttgggg aacaggcgca gataaatggc atccagactg tcagactcac ggttggtata
     7501 gcggattgac tcgtgaccct gcaccgacaa ataatcggct gcgatggtga tatccagccg
     7561 gtattcggtg gcgccgggga gttctgccag tataggctgg gcagattcaa tcaagccgga
     7621 gcgatagatc tctcgcccct cggcaaattg aggcgtctcc aaagctggag gtgtggaggt
     7681 caaaactggc gccggagtgc ggcaggcaga cagaagcatg acgaggagga taaatccaga
     7741 cagcagaccc agggttttat tcatggcaaa ctccaaaaag gggattaata tcaagattat
     7801 gtcaggtgga ggagaatgtc aaggctctgg ttaaaatgct gactgaagct tgcacttgaa
     7861 aacaggccgt ataacttgtg actggttgag ccgttacaaa aaataaaagg acgggcgcag
     7921 aggcccgtcc taaaaaatct tgtggggttg ggggtgggat taatcggcgc ccacaatacc
     7981 gtaacgcttc tgcagcagat catcggcagg ttggcccagc atcagatgca gaagtaaatc
     8041 tttctgggtg cggacaaagg gta
//
LOCUS       DAOPVS010000087         7949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9102_length_7949_cov_56.940081, whole genome shotgun sequence.
ACCESSION   DAOPVS010000087 DAOPVS010000000
VERSION     DAOPVS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9102_length_7949_cov_56.940081"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKL11_07700"
     CDS             <1..324
                     /locus_tag="PKL11_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ13525.1"
                     /translation="QALQIARSEPERREALWRNIRQARAGLRDLGWALPDTPVAIQCL
                     GARPGLDLGALKAGLFERGIAVAHVTTYSSTPVGGALRIAIFATHTPAQIDRLVKTLA
                     ELISV"
     gene            374..1114
                     /locus_tag="PKL11_07705"
     CDS             374..1114
                     /locus_tag="PKL11_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13526.1"
                     /translation="MMNKRWFSLFTILVLAALLLGVAQPVPTSAEALTAEPTLTPQVS
                     TDLRHDTSIALIDMTPLPAPEGETPERYSPQRSRLEETAGLNNMTDPAIVQSDFGLQT
                     IPTPLTNFDGVLSRNNVHPPDTNGDVGPNHYMQWVNLSLQIWDKAGNSVFGPVNGNTI
                     WSGFGGPCETTNSGDPIVLYDELAGRWFISQFSVNEPYYQCIAVSQTADPTGAWHRYQ
                     FLYSSSVMNDYPKFGVWPDGYYMSANQF"
     assembly_gap    1285..1384
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1385..4354
                     /locus_tag="PKL11_07710"
     CDS             <1385..4354
                     /locus_tag="PKL11_07710"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ13527.1"
                     /translation="NFGTYATLVSNVTVDVGSDRAGVYWFELRDTGSGYNMYQQGVFA
                     PADGNHRWMGSAAMDAGGNIALVYSVSSAAMHPAIRYAGRLATDPLGQMTQGEAELYQ
                     GPASSTSTYYRWGDYSMISVDPLDGCTFWMTSEYYATTGIRTWATRIGSFRFPSCAPV
                     ASGTLEGVVTDADTGEPISGATVEVSYYSLLTEADGSYSTLISEGTYDVTASSEMYLP
                     QTVSVTINEGETTTQNFALQPRPASVITGTVTDGGDHAGMPIYARVEVSGSGESPVFT
                     DPVTGEYSITLVEGLEYTFTVTAITPGYTGETRQVTAPTGGGTVNFALVPDLTLCTAP
                     GYHFESTGLFENFETGALPSGWTVEDLEGTGEVWRFDNPGHRVNNTGGSGEFAIIDSG
                     FYGGTGHQDTILKSPVLDLSALATVDLQFNTDYDYRSGDFASVDVSNDAGATWHNAWY
                     RSSDARGPRTETIDISTLAAGQNDVQIRFHYGNGSFDFFWAVDNVMIGQAPECVAVAG
                     GLIAGQTLDANTDIVLNDTVVSSAAHQTVSFATPDDPNIGDGYYILFVPADHQTVTAT
                     HTGGYGTLQQVVTVSPDDGTALDLLIPAGQVTADPIIITRTLGQRMLSQAVVTLSNNG
                     GAEAPFTVLEMSGLPLPAPQPTGPFAAAGRHVGPKRLSEPTGAYVPLHDYPAAPDWAG
                     AGNVLSSFNTGLQGAWGLSQMPDGSFWVGSIVPTGGDGLNYHFVDGSATGEVMDTGFA
                     DPWAADLAYNARTGMLWQVGLTDGDCIYELDPQSGLATGNKLCPGFGVSQRALTYDPR
                     SNTFYSGSWNDAILYHFDTNGRMLDSIDLNLEIAGLAFNPDSGHLFVLQSAPASASDV
                     YVLDPAQDYALLGRYDIPALGDYAAGGLEMSCNGSLWIVDQTTNQVLETASGEAHACT
                     WADVSWLSVTPTSGAVAANGSTDLSVLINAGTLPLGTYEAHLLINTETPYTVTPVSIT
                     LNVVDGGALIFLPVIVR"
     gene            complement(4454..5500)
                     /locus_tag="PKL11_07715"
     CDS             complement(4454..5500)
                     /locus_tag="PKL11_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13528.1"
                     /translation="MKRLSIILLIVTLVLALTSCTPTTTPITPLPSDAPDAAEQPVTT
                     IPLSGPLAADNAEISGLAWASTAAGDYLLLLPQYPGRFGDAIFALPKADLLAFLDGQS
                     SEPLVPQPIPFDAAGIPDQLDRFEGYESITVSGSQVYLTIEARPGNQPMAYLVTGQLA
                     DDLSQITLDNQYLAEIPTQAGLENMTDEAVLIWQESVLTFHEANGVNVNPQPVAHSFD
                     PLTLQPQTDVSFPNIEYRITDVALPDESGRFWAINYFFPGDTKLKPADDPLVARFGAG
                     PTHAASPVVERLVEFELSDSAIILTDTAPIALQLLPDEEARNWEGLALLDARGFLLAT
                     DKYPHTILAFVARP"
     gene            5543..6370
                     /locus_tag="PKL11_07720"
     CDS             5543..6370
                     /locus_tag="PKL11_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13529.1"
                     /translation="MQTPAPTPRRTLPQLLGWNRTSYALMSGFVAIVLLIIYVWWPLV
                     VDYFSQLDPRYPVWVQLDWLLIGIFLVMSLLIMAKADLRTDALIVFVGMCGGLAIEGW
                     GTQTHIWTYFTLERPPLWIIPAWPIASLSIDRLVRLIQQLTTRWPEKVFRGIYWPVFG
                     AFYVLMWIFVWPTLDKSLTILALLAVGLILLSPTDYRLAVLTFAAGSGLGYFLELWGT
                     TRYCWMYYTQQTPPLFAVLAHGLAAVAFWRVGRLLVMVWGKIRRREAAAAEQNANAV"
     gene            complement(6393..7196)
                     /locus_tag="PKL11_07725"
     CDS             complement(6393..7196)
                     /locus_tag="PKL11_07725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ13530.1"
                     /translation="MACSNPPALSEVIGWARQAGALLQAGLGKKHTIEYKGPVNLVTE
                     MDQASEALLIDLIRSRCPDHKIITEESGALAGADGCCWYIDPLDGTTNYAHGLPIYAV
                     SIGFVANGIQQLGVVYDPSRDECFSAEHGRGAWLNDQPIHVTTETELQHSLLVTGFPY
                     SILTSNNNNLGYFNRFSLRSRAVRRLGSAALDLCYVAMGRFDGYWELLLQSWDIAAGS
                     LIAAEAGAHLTDMQGGPNFMSPPHSLIVANPAIHAQMLAVIQETPMEVF"
     gene            complement(7273..>7949)
                     /locus_tag="PKL11_07730"
     CDS             complement(7273..>7949)
                     /locus_tag="PKL11_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ13531.1"
                     /translation="IIISFCLLAFSPKSRYNTLMNVPFGPILIVEDNPNVLELLEVTL
                     RFKGYPVVTAVNGQDALEKAAKERPALIITDILMPRMDGYSLAHNIRKNPLTAKIPII
                     FISATYITPEDKAFALSLGAVRFIEKPIDAEDFLLTVAEILTQGIATVPRPMEDTKFY
                     QGYRERLENKLRYKNTQIARTERLLQNLPPEQKPAFEALLAQAIHDRDDIQTELDQLY
                     RILGQQ"
BASE COUNT         1609 a         2113 c         2470 g         1657 t
ORIGIN      
        1 caggcgctgc agattgcccg atcagaaccc gagcgccgtg aagccctgtg gcggaatatc
       61 cggcaggccc gcgccggact gcgtgatctg ggttgggcgc tgcctgatac cccggttgcc
      121 attcaatgcc tgggggcccg ccccggcctg gacctggggg cgctcaaagc cgggctgttt
      181 gaacgcggca ttgccgtggc gcatgttacc acctacagca gcaccccggt tggcggcgcg
      241 ctgcgaattg ccatctttgc cacgcataca ccggcgcaga ttgaccggct ggtgaaaacg
      301 ttggcagaac tgatctctgt ttaatcgccg gggcaattgc cccggtttat ttttcccata
      361 cctggagcaa aacatgatga ataaacgctg gttctcgcta tttaccattc tggttctggc
      421 cgcgctgtta ttgggggtgg cgcagccggt tccaacctca gccgaggcac tgactgctga
      481 gccaaccctg accccacagg tcagcacgga cctgcggcac gacacctcga ttgcattgat
      541 tgatatgacc ccgctgcccg cacctgaggg tgaaacacct gaacggtaca gcccgcagcg
      601 gtctcggtta gaagaaactg ccgggctcaa caacatgacg gacccggcga ttgtgcaaag
      661 cgattttgga ctgcaaacca tccccacccc gctgaccaat tttgacgggg tattgagccg
      721 caacaatgtg catccgccgg acaccaacgg cgatgtgggg cccaatcact acatgcagtg
      781 ggtcaacctg tcgctgcaaa tctgggataa agccgggaac agcgtttttg gcccggtaaa
      841 cggcaatacc atctggtcgg gttttggtgg cccgtgtgaa acgaccaaca gcggcgaccc
      901 gattgtgctg tatgatgaac tggcgggacg gtggtttatc agccagttct cagttaacga
      961 accgtattac cagtgtattg cggtttcaca gacggctgat ccaacgggcg cctggcaccg
     1021 gtaccagttc ctgtacagca gctcggtgat gaacgattac cccaagttcg gcgtctggcc
     1081 ggatggctat tatatgagcg ccaaccagtt ttaaggtgac tcgggggtag gcgtggtggt
     1141 gtatgaacgt gagcggatgc tgcaggggtt gagcgcccgt caggtgtact tcaacctgag
     1201 caacaccacc tcgtgggcct gggcgctgct accctctgat tacgatggca cgccgccgcc
     1261 ggctggcgaa cccaattact tcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnaatttt ggcacctatg ccacgctggt cagcaatgtg acagtggatg tgggcagcga
     1441 ccgcgccggg gtgtactggt ttgagctgcg cgataccggc agtggctata acatgtatca
     1501 gcaaggggtc tttgctccgg cagatggcaa ccaccgctgg atgggcagtg cggcaatgga
     1561 cgccggcggc aatatcgcgc tggtgtacag cgtatccagc gcggccatgc acccggctat
     1621 ccgctatgcc gggcggctgg caaccgaccc gctggggcaa atgacccagg gtgaggcaga
     1681 gttatatcag ggtccggctt catccaccag cacctactac cgctggggcg attacagcat
     1741 gatctcggta gatccgctgg atggctgcac cttctggatg acttcggaat attatgccac
     1801 caccggcatc cgcacctggg ctactcgaat tggctccttc cgcttcccaa gctgcgcgcc
     1861 ggtggcatct ggcacgctgg agggcgtggt gaccgatgcc gacacgggag aaccaatctc
     1921 gggagctaca gtagaagtga gttattactc gctattgacc gaggcagatg gcagttacag
     1981 cactttgatt tccgaaggaa cctatgatgt caccgcgtcg tcagagatgt acctgccgca
     2041 aacggtcagc gtgacgatca atgaagggga aaccactact caaaactttg ccctgcagcc
     2101 ccgcccggct tcggtcatta ccggaacggt gacggatggc ggcgaccacg cgggcatgcc
     2161 gatctacgcc cgggtggaag tcagcggttc gggtgaaagc ccggtgttca ccgacccagt
     2221 cacaggcgaa tacagcatca cgctggtgga agggctggaa tacaccttca cggtaacagc
     2281 catcacgccg ggatacaccg gggagacacg ccaggtgaca gcccccacgg gcggcggtac
     2341 ggtgaatttt gcgttggtgc ccgacctgac gctttgcacc gcccccggct accactttga
     2401 atctacgggg ttgtttgaaa attttgaaac cggtgctttg ccatcaggct ggacggtgga
     2461 agacctggaa gggaccggtg aggtctggcg gtttgataac cccggccacc gggtgaacaa
     2521 caccggcggc agcggcgaat tcgcgatcat tgacagcggt ttttacggcg gtacgggaca
     2581 ccaagatacc atcctcaagt ctccggtgct ggatttgagc gcgctggcga ccgtggattt
     2641 gcagttcaac accgattacg attaccgcag cggcgacttt gccagcgtgg atgtgagcaa
     2701 tgatgcgggc gccacctggc ataatgcctg gtaccgcagc agcgatgccc gcgggccgcg
     2761 cacggagaca atcgatatct caactctggc tgccgggcag aatgatgtgc agatccgttt
     2821 ccattatggc aacggctcgt ttgacttctt ctgggcggtg gataatgtga tgattggtca
     2881 ggcgccggag tgtgtggctg tggctggcgg tctgattgca gggcaaacgc tggatgccaa
     2941 cacagacatc gtattgaacg acacggttgt cagttcagca gcccatcaaa ccgtctcgtt
     3001 tgccacgccc gatgatccca acattgggga tggatattac atcctgtttg tgcccgctga
     3061 tcatcagacc gtgacggcga cgcatacggg gggttacggc acactgcagc aagttgtgac
     3121 agtgagcccg gacgatggca cagcgttgga tctgctgatt ccagccggac aggtcactgc
     3181 cgatccgatt atcatcaccc ggacgctggg acagcggatg ctgagtcagg cagtcgtcac
     3241 gctgagcaac aacggcggtg ccgaggctcc ctttaccgtg ctggaaatga gcggtctgcc
     3301 tctgccggcg ccgcaaccca ccggaccctt tgcggcagcg gggcggcacg tgggccccaa
     3361 acgtctgagc gaaccgaccg gcgcgtatgt gcccctgcat gattatccgg cggcacccga
     3421 ttgggccggc gcgggtaacg tgttgagcag ctttaacacc ggtctgcaag gcgcctgggg
     3481 gctgagccaa atgcccgatg gcagcttctg ggtcggctcg attgttccga ccggcggcga
     3541 cgggctgaat taccattttg tagatggttc agccacgggt gaagtgatgg ataccggctt
     3601 tgccgatccg tgggccgccg acctggccta taatgcgcgc acggggatgc tctggcaggt
     3661 ggggctgacc gatggcgact gcatctacga attagacccg caatctgggc tggcaaccgg
     3721 aaacaagctg tgccccggct ttggggtctc gcagcgggcg ctgacctatg acccgcgcag
     3781 taatacgttc tactctggtt cgtggaatga cgccattctg tatcactttg ataccaacgg
     3841 acggatgctg gactcgattg acctgaatct ggaaatcgcc gggctggcct tcaacccgga
     3901 cagcggccat ttgtttgtac tgcaaagcgc ccctgccagt gcttcggatg tctatgtgct
     3961 ggacccggcg caggactatg cactgctggg gcgctatgac attccagcgc tgggagatta
     4021 tgctgccggc ggcctggaaa tgagctgtaa tgggtcgttg tggattgtcg atcagactac
     4081 caatcaggtg ttggagaccg cctcgggtga ggctcacgcc tgtacctggg cggatgtgag
     4141 ctggctgtcg gtgacgccga caagcggcgc agtggctgcc aatggcagca ccgatctgag
     4201 cgtattaatc aacgctggca ctctgccgct gggaacttac gaagcgcacc tgttaatcaa
     4261 tacagaaacg ccctacacag tgacgccggt atccatcact ctgaatgtgg tggatggggg
     4321 ggcgttgatc ttcctgccag taatcgtgcg gtaaggctgg aaacccgcag tcaaaaccct
     4381 gctgtaaagt cagaagggca ttaaatcaaa ggagcgcaag acaacttgcg ctcctttttg
     4441 attagagccg atttcagggc cgggcgacaa aagccaggat ggtatggggg tatttatcag
     4501 tcgccagcag gaagccgcga gcgtccagca gggccaggcc ctcccagttg cgggcctctt
     4561 catcgggcaa gagctgcaaa gcaattgggg cggtgtcggt caggatgatg gcgctgtcgg
     4621 ataattcaaa ttccaccagg cgctcaacca ctggtgaagc ggcatgggtg ggccctgcgc
     4681 caaaacgcgc aaccagagga tcatctgcag gcttcaattt ggtatcgccg gggaaaaagt
     4741 agttgatggc ccaaaaccgc ccggactcgt cgggcaaggc gacatcggta atgcggtatt
     4801 cgatgttggg gaagctcaca tcggtctgcg gctgcagggt cagcgggtca aagctgtggg
     4861 ccaccggctg cgggttgacg ttgacgccgt tggcttcgtg gaaggtcagc accgattcct
     4921 gccagatgag aaccgcctca tcggtcatgt tttcaaggcc ggcctgggtg gggatttcgg
     4981 cgaggtattg attgtcgagg gtaatctggc ttaaatcatc agccagctgc ccggtgacca
     5041 gataagccat gggctgattg cccggacggg cctcaattgt cagatagacc tggctgcccg
     5101 agacggtgat ggattcgtac ccctcaaagc ggtcaagctg atctggaatg cccgccgcat
     5161 caaaggggat gggctgcggc acgagcggtt cggaggactg tccgtccagg aaggcaagca
     5221 ggtcggcttt gggcagtgca aagatggcgt cgccaaagcg gccagggtac tggggtaaaa
     5281 gcaacaaata atcacccgcg gccgttgagg cccaggccag gccggagatt tcagcattat
     5341 ctgcggcgag cggcccgctg agcgggatgg tggtgacggg ctgctcagcg gcatcaggcg
     5401 catctgatgg aagcggggtt atcggtgtgg ttgtaggtgt gcaactggtc agcgcaagca
     5461 ccagggtgac aatcaggaga atgattgaga ggcgtttcat gggatttttc ctttgtgtcc
     5521 attataatag gccccagtcc acatgcaaac acccgccccc accccgcgcc gcaccctgcc
     5581 ccaactgctg ggctggaacc gcaccagcta tgcgctgatg agcggatttg tggcgattgt
     5641 gctgctgatt atctacgtct ggtggccgct ggtggtagat tatttttcac agcttgaccc
     5701 gcgctacccg gtatgggttc agctcgactg gctgctgatt ggcatttttc tggtgatgag
     5761 cctgctcatc atggctaaag ctgacctgcg aactgatgcg ctgattgtat tcgtggggat
     5821 gtgcggtggg ctggcaattg agggctgggg gactcagacg cacatctgga cctactttac
     5881 gctggaacgc ccgccgttgt ggatcatccc ggcctggccg attgcctcgc tgtcgattga
     5941 ccggctggtg cggctgattc agcagctgac gactcgttgg ccggagaagg tctttcgggg
     6001 catctactgg ccggtgtttg gggcatttta cgtgctgatg tggatttttg tgtggcctac
     6061 gctggataag tctttgacca ttctggcact gctggccgtg ggcttgattc tgctctcgcc
     6121 aaccgattac cggctggccg tgctgacctt tgcggcaggc tcggggctgg gttatttttt
     6181 ggagctgtgg ggcaccacgc gctactgctg gatgtattac acgcagcaaa ccccaccgct
     6241 gtttgcggtg ctggcgcatg ggctggcggc ggtggcgttc tggcgggtgg ggcggctgct
     6301 ggtaatggta tggggaaaga tccggcgccg ggaggcggca gcagctgaac aaaacgccaa
     6361 cgcggtgtaa cccccagctc gatcctggcc tgttagaata cttccatggg tgtttcctga
     6421 atgacggcca acatttgagc gtggatagcg ggattggcga caatcaacga atggggcggg
     6481 gacatgaaat tggggccgcc ctgcatatcg gtaaggtgag cgccggcttc ggcggcgatg
     6541 agcgacccgg cagcgatatc ccagctctgg agcagcagtt cccagtaacc atcaaagcgg
     6601 cccatggcca catagcacag atccagcgcg gcagaaccca gacggcgcac cgcccggctg
     6661 cgcagcgaaa agcggttgaa gtagccgaga ttgttgttat ttgaagtcaa aatgctgtag
     6721 gggaagcccg tcaccagcag gctgtgctgg agttcggttt cggtggtcac gtggatgggc
     6781 tgatcattga gccaggcgcc gcggccatgc tccgcgctga agcattcatc ccggctggga
     6841 tcataaacca cgcccaactg ctggatgccg ttggccacaa agccaatcga gaccgcgtaa
     6901 atgggcaggc catgcgcata gttggtggtg ccgtccaggg ggtcgatata ccagcagcag
     6961 ccatctgcgc cagccagggc gccgctttct tcggtgatga ttttatgatc cgggcagcgg
     7021 ctgcggatca ggtcaatcag cagcgcctca gaagcctgat ccatttcggt gaccaggttg
     7081 acggggcctt tgtattcgat tgtatgcttt ttgcccagac cagcctgaag cagtgcgcca
     7141 gcctgacggg cccaaccgat aacttcggaa agtgcagggg gattggaaca agccataaga
     7201 gagtaatgtc gctttcatta aaacctcccg caagccaggg gccggcggga ggcgggatgg
     7261 attaagtcgg gattactgct gccccaggat gcggtagagc tgatccagct cagtctggat
     7321 gtcgtcgcgg tcgtgaatgg cctgagccag aagcgcttca aaggcgggtt tctgttcggg
     7381 gggcaggttt tgcagcaggc gctcggtgcg ggcgatctgc gtattcttat agcgcagttt
     7441 gttttccagg cgctcgcggt agccttgata gaacttggta tcttccattg ggcgcggcac
     7501 agtggcaatg ccctgggtta gaatttcggc cacggtgagc aagaagtctt cagcgtcaat
     7561 cggtttttca ataaatcgaa ccgcccccag gctgagcgca aaggctttat cttcaggtgt
     7621 aatgtaggtg gcagaaataa agatgatagg aattttggcc gtgaggggat tcttacggat
     7681 gttatgcgcc agcgaatatc catccatgcg gggcattaaa atatcagtaa tgatcaatgc
     7741 cgggcgctct ttggctgctt tttcaagcgc atcctgccca ttgacagccg tcacaaccgg
     7801 ataacccttg aagcggaggg taacctccaa caattcaagg acgttggggt tgtcttcaac
     7861 aattaagatc gggccaaaag gtacattcat gagtgtattg taacgagatt taggggaaaa
     7921 ggcaagcaag cagaatgaga ttatgatga
//
LOCUS       DAOPVS010000088         7925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9139_length_7925_cov_57.449555, whole genome shotgun sequence.
ACCESSION   DAOPVS010000088 DAOPVS010000000
VERSION     DAOPVS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9139_length_7925_cov_57.449555"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PKL11_07735"
     CDS             complement(<1..1014)
                     /locus_tag="PKL11_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13532.1"
                     /translation="MSKSPGFRWLLLVFLLCLTLAACTPAPAPVTLTPTRLLATPQPT
                     ATPSPQPSHTFTSPPATTSTPDIAATQVRQTADASATQSAGFPVTCKGFRVGEPSFSP
                     GGNWMAVSCGYQHDQTLDIVNRDGRQWILQFAELVPDHDGMGGLSPVHWTGDETYLYF
                     TAYKSYDGGGPCFYYPTVNGLYQINLDSGTVVTILPETDVPGSYEVAISPNSRWVAYT
                     NNLDQPRILDLRTGETIEIEVGKYKTGSLTWSPDGNYLAYARCEIDQDPLKVKNSGVQ
                     IFAIKTRTLTTPLEVAGDWVIIESGEGRDFLTIRIENIETFSIRYQFYDWSTGQLTTP
                     TPAP"
     gene            1235..2263
                     /locus_tag="PKL11_07740"
     CDS             1235..2263
                     /locus_tag="PKL11_07740"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNZ13533.1"
                     /translation="MALSGTVDKTTLPAMSELKTRLAPFARANTRVALRQLIVTLLLY
                     AAGWAAMYFSLQVGYWLTLLLAVPVAGLLVRIFIFFHDCGHNSFFPSTKLNRRVGFWL
                     GVLVFTPGEQWWRSHAIHHATSGNLDKRGVGDVTTLTVSEYRQLSPWGRLGYRLFRHP
                     LIMFLLGPIWMFLISHRLPLPRHGQKETRSVIWANLAILGLAAGLSLLLGFKEYVLIQ
                     LPVIWLGGMAGIWMFYIQHQFTGVYWARTPQWNYVASAFQGASYYRLPRVLQWFTGNI
                     GFHHIHHLNPLVPNYALDAAYHSDGLLRQSPTLNIRQSLASIPLALYDEAAGQMVSFR
                     QARQNAKP"
     gene            2285..3082
                     /locus_tag="PKL11_07745"
     CDS             2285..3082
                     /locus_tag="PKL11_07745"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNZ13534.1"
                     /translation="MYNVGVDAPTLTAALRGLPVPQIRYRPVTGSTNADALAWAAVGA
                     PDGCLVVADEQTAGRGRLDRRWYTPPGAALAFSLILRAAPSELPHLGLFSPLAGLALW
                     QTLLAYGLPAQIKWPNDVLLAGRKTAGILAEADWTDAAAPVVVLGVGVNVLPEAVPPP
                     DLLRFPATCVSAALGAPVDRVALLSEILSAFFALRPRLGSPQMLQTWQHALAFLGETV
                     YIENQGQPVKSGVLLGVDATGALRLQPSDGPELTIPAGDVSLRPAHF"
     gene            3098..3337
                     /locus_tag="PKL11_07750"
     CDS             3098..3337
                     /locus_tag="PKL11_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13535.1"
                     /translation="MLEDLRNSANFNDEPETPPQPPKPAPERRAAPRPAARFKDPLIL
                     GMTAVQRLIVALLLFFSTCLLGALCLVITGRVYLP"
     gene            3467..3886
                     /locus_tag="PKL11_07755"
     CDS             3467..3886
                     /locus_tag="PKL11_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNZ13536.1"
                     /translation="MQITRQADYALRAILFLARQDSNVRSATSQIAEVQHIPPSFLAK
                     IISQLAIAGLIHTSRGARGGVTLARPAEEITVLEVVEAIDGPMELNQCTLSSAVCPFG
                     PACPLRALWCDTQDVLIERLRTTTFGQIVANERAAQE"
     gene            3920..4177
                     /locus_tag="PKL11_07760"
     CDS             3920..4177
                     /locus_tag="PKL11_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13537.1"
                     /translation="MKTGSSIGRPWVVVLYDGSVMLDWGDGLFQDITTGEFARLTEAQ
                     ISHTVRTEELEYLRASGQIDGFDDRCVYFHGLPERPQSDEG"
     gene            complement(4256..5059)
                     /locus_tag="PKL11_07765"
     CDS             complement(4256..5059)
                     /locus_tag="PKL11_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNZ13538.1"
                     /translation="MRETLKKILPAPALNLLRETRDGLARLKDWPAATFHPWRRQTRD
                     RLGALRNLHAGQRCFILGNGPSLRQTDLTKLRGEYTIGLNRIFLAFPEMGFQTSYLVS
                     INDLVIEQSAAELQALAMPRFFAWRAHRWLQPADDLYFLYTTYTGPRFARDVRGRLWE
                     GATVTYVALQLAFYLGFETVILVGVDHNFVTQGKPNTTIVSEGDDPNHFHPGYFGKGF
                     RWQLPDLDTSERGYLLARRAYEQAGRRVLDATIGGKLQIFEKVDYNGLF"
     gene            complement(5067..5831)
                     /locus_tag="PKL11_07770"
     CDS             complement(5067..5831)
                     /locus_tag="PKL11_07770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ13539.1"
                     /translation="MSDPKRIMLITGAAGGIGRATVQLFAQNGWTVIGVDRAPFGEPF
                     PSDGFFIQADIADPAALESIIAQTRSFTHTLDALVNNAALQVAKPIVETSVAEWDLTI
                     ASNLRSAFLGAKLAHPLLKAGGGGAIVNMSSVHAVQTSANIAAYAASKGGLLALTRAM
                     AIEFAPDQIRVNAVLPGAVDTPMLRAGLGRGHVGDGSITERLDNLARKTVNGRVGLPE
                     EIAHAVYFLADGTQSSFMTGQALIVDGGATARLSTE"
     gene            complement(5864..7855)
                     /locus_tag="PKL11_07775"
     CDS             complement(5864..7855)
                     /locus_tag="PKL11_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13540.1"
                     /translation="MSRRARPDWLPYAVTLGLLLLLAVLAYLPLILQLGFYKDDWHVI
                     WAGSSFGPGRIIDEFMIDRPFMGRLYAKVFILLGSAPLNWHLFAFAARFASVVGFYAL
                     LRLLWPARRLEAAAAAALFILYPGFLQQPNANTFQNHFIGYALAIFSIALTVFALTTA
                     RRNWLQAAALALGAVLAGGYLLIYEYMIGLEAVRLLLIIYVLRRQGCGWRKWLPKTAL
                     IYLPYLLAAGAFLYWRLFIFHSTRPATDAGALWALYTGQPLAMLGQLSIETLKDLVET
                     TFLAWGVPLYQLAVNAPAGQMAAGLLAAAAGAGLWWFFAARQERPAEAPVKDDFGRAA
                     IVLGALMVLVTLLPVLASNRNVYFRDQFDRYTLQATLGVALLLTGLIFGAARIRAARG
                     WVAVLIAIALLTHTLNAIYWGQFWQVQRSFWHQLAWRAPQLEDKTLLMAYLPPNYRLA
                     EDYEIFSPANLIYHPGEPRLAIEAEVLNADTAALLTAGVTGSLRSSRTFEFERDFTRA
                     LIVSWADPQVCVHLLDPAQPDLLAAAAADPLLQLTAPYSHPQQVVVDAPEPVVYPAVF
                     GPEPAHGWCYYYQKASLARQQADWIEAARLGDAARQQGLAPADPLEWLPMLQGYLHEN
                     RTTEAAELAAFIRQSAAGPALCAQYRAGLDPLTVPLLCP"
BASE COUNT         1510 a         2450 c         2477 g         1488 t
ORIGIN      
        1 tggcgcaggc gtgggcgtgg tcaactgtcc ggttgaccag tcgtagaact ggtaacggat
       61 tgaaaaagtt tcaatatttt caatcctgat ggttagaaaa tcccggcctt ccccactttc
      121 aataatgacc cagtccccgg ctacttccag cggtgtggtc agtgttcgcg ttttgatggc
      181 aaagatctgt acgccagaat tttttacttt cagggggtcc tggtcaattt cacacctggc
      241 gtaggccaga taattcccat cgggtgacca ggtcagactg ccggtcttgt atttaccgac
      301 ttcaatctcg atagtttctc cagttctcaa atccaggatc ctgggctggt cgagattatt
      361 tgtgtatgca acccagcggc tgttgggcga aatggcaacc tcataactgc ccggcacgtc
      421 tgtttcaggc aggattgtga cgactgtgcc ggaatccagg ttgatttgat aaagaccatt
      481 gacagtggga tagtaaaagc aaggcccacc gccatcatag gatttgtagg ccgtgaaata
      541 cagataggtt tcatcacctg tccaatggac cgggcttaag ccacccatcc cgtcatggtc
      601 tggcaccagt tcggcgaact gcaaaatcca ctgtcgtcca tctcgattca caatgtctaa
      661 tgtctgatca tgttgatatc cgcacgagac agccatccaa tttccacccg gcgaaaaaga
      721 aggttcccct actcgaaaac ctttgcaggt cactggaaat ccggcgctct gagtggctga
      781 ggcatctgcc gtttgccgga cctgggttgc ggcaatatct ggcgtgctgg ttgtggctgg
      841 tggtgatgtg aacgtgtgtg agggctgcgg cgagggcgtg gctgtgggct gcggcgttgc
      901 cagcagacga gtgggggtga gggtgactgg tgcgggcgca ggggtgcagg ctgccagcgt
      961 caggcacagc aaaaacacca gcagcagcca tcgaaatccg ggggatttgg acatacttgg
     1021 ctccatcatt ttggggagag gggataaaaa caacgggctt gaaacacacc ctatcgttta
     1081 ttatatcgct ttaacatcca aaccctggcg gggttttcct gccgggtcat actggcacaa
     1141 tatgcctcgc ggcacatgcg ccgccggggg catcgggcta tactcaaact ggcgcagttc
     1201 catctgcccg cattctcttc caccgaggtt aaagatggcc cttagcggta ctgttgataa
     1261 gaccaccctg cccgccatgt cggagcttaa aacccggctg gcgccgtttg cccgcgccaa
     1321 tacccgcgtg gcgctgcgcc agttgattgt gacgctgctg ctgtatgccg ccggctgggc
     1381 ggcaatgtat ttcagcctgc aagtgggcta ctggctgacg ctgctgctgg cggttccggt
     1441 ggccgggctg ctggtgcgca tcttcatatt ctttcacgac tgcggccata actcgttctt
     1501 cccgtccaca aaacttaacc ggcgggtggg tttctggctg ggggtgctgg tctttacccc
     1561 cggtgagcag tggtggcgct cccatgccat ccatcacgcc accagcggca acctcgacaa
     1621 gcgcggcgtg ggcgatgtga ccaccctcac cgtgagcgaa taccgccagc tttcaccctg
     1681 gggccggttg ggctaccgtc tgttccgcca cccgttgatc atgttcctgc ttggccccat
     1741 ctggatgttc ctgatctcgc accggctgcc gctgccgcgc cacggccaaa aagagacccg
     1801 ctcggtcatc tgggccaacc tggccattct ggggctggcg gcgggcctca gcctgctgct
     1861 gggcttcaaa gaatacgtcc tcatccaact gcccgtcatc tggctgggcg gcatggctgg
     1921 catctggatg ttttacattc agcatcaatt taccggtgtt tactgggcgc gcacaccgca
     1981 gtggaactat gtggcctccg cctttcaggg ggcctcttat taccggctgc cgcgggtgct
     2041 gcagtggttc acgggcaata ttggctttca ccacatccat cacctcaacc cgctggtgcc
     2101 caactacgcc ctcgatgctg cctatcactc cgacggcctg ctgcgccagt cgcccaccct
     2161 caacatccgg cagagcctgg cctccatccc gctggcattg tatgacgaag ccgccgggca
     2221 gatggtcagt tttcggcagg cgcgccaaaa cgcaaaacct taacgttacc ccccgcctgg
     2281 tttgatgtac aatgtgggtg tggatgctcc aaccctcacc gccgcgctgc gcggcctgcc
     2341 tgttccgcaa atccgttacc ggcccgtcac cggctcgacc aatgccgatg cgctggcctg
     2401 ggcggctgtg ggtgcgccgg atggctgcct ggtggtggcc gatgagcaaa ccgccgggcg
     2461 cggccgcctg gaccggcgct ggtacacccc gccgggcgcg gcgctggcct tcagcctgat
     2521 tctgcgcgcc gcaccatccg agctgcccca cctgggcctg ttttcgccgc tggccgggct
     2581 ggcactctgg caaacgctgc tggcttacgg cctgcccgcg cagatcaaat ggcccaatga
     2641 tgtgctgctg gctggccgca aaacggccgg catcctggcc gaggccgact ggacagacgc
     2701 cgccgcgccg gtggtggtgc ttggggtggg cgtcaacgtg ctgccagagg ccgtgccgcc
     2761 gccagacctg ctgcgctttc cggccacctg tgtaagcgcc gccctgggcg ccccggtgga
     2821 ccgggtggcc ctgctgagcg agattctgag tgcctttttt gccctgcgcc cccggctggg
     2881 cagcccgcag atgctgcaaa cctggcagca tgcactggcg tttctgggcg aaaccgtgta
     2941 tattgagaac cagggccagc ccgtcaaatc gggcgtgctg ctgggcgtgg atgccaccgg
     3001 cgccctgcgc ctgcagccct cagatggccc cgagctgacc attcccgccg gcgatgtgag
     3061 cctgcgcccg gcgcattttt gaaccggaga tttaccgatg cttgaagacc tgcgcaactc
     3121 ggcaaacttt aatgacgaac ccgaaacccc gcctcagcca cccaagcccg ccccggagcg
     3181 tcgcgccgcg ccgcgcccgg cagcccgctt caaagacccc ctgatcctcg gcatgacggc
     3241 tgtccagcgg ttgattgtgg cgctgctgct tttcttctcc acctgcctgc tgggcgccct
     3301 ttgcctggtc atcaccgggc gggtctatct cccctgagaa ctttccttgc cgaaagtcat
     3361 tcggcaggga tgatgtttat tacttgtcgg ataattatga ctggaataaa ctcatttaga
     3421 gtggagtaat ctgaccctat tttctttttt cagcaaggaa gacatcatgc aaatcacccg
     3481 ccaagccgac tatgcgctcc gtgccattct tttcctggcc cgccaggatt caaacgttcg
     3541 ctctgccacc agccagattg ccgaagtgca gcacattccg ccatcttttc tggcgaagat
     3601 catctcgcag cttgccattg ccgggctcat ccacacctcg cgcggcgcgc gcggcggcgt
     3661 gacgctggcc cgccccgccg aagagatcac ggttttggaa gtggttgagg cgattgacgg
     3721 ccccatggaa ttgaatcagt gcacgctgtc ttcggcggtt tgcccgtttg ggccggcctg
     3781 cccgctgcgc gccctctggt gcgacaccca ggatgtgctg attgaacgtc tgcgcacgac
     3841 cacgtttggc cagattgtgg ccaacgaacg ggcggctcaa gaataaaact ggagaactca
     3901 ttttcaaagg gggggcagca tgaaaacagg cagcagcatt ggtcgtccgt gggtggttgt
     3961 gctctatgac ggcagtgtga tgttggattg gggtgatggg ctgtttcaag acatcaccac
     4021 cggcgagttt gcgcgcctga ccgaggcgca gatcagccac accgtccgca ccgaagagct
     4081 ggaatacctg cgcgccagcg gtcagattga tggcttcgat gaccgctgcg tctattttca
     4141 cgggctgccc gagcgccctc agtctgatga gggctgagag gtccgttcca ggtgtgaggc
     4201 gcgcccctgc ccaacccatc tgggccctgg cacaggcagc gccaaaacct atcccctaaa
     4261 acaacccgtt ataatccacc ttctcaaaga tttgcagctt gccgccaatg gtggcatcga
     4321 gcacgcggcg gccggcctgt tcgtaggccc ggcgggccag cagatagccc cgctccgagg
     4381 tgtccaggtc ggggagctgc cagcggaagc ccttgccaaa atagccgggg tggaagtgat
     4441 tggggtcgtc gccttcggag acgatggtgg tattgggctt gccctgggtc acaaagttgt
     4501 gatccacgcc aaccagaatg accgtctcaa agccgaggta gaaggccagc tgcagggcca
     4561 cataggtcac ggtggcgcct tcccacagcc gcccgcgcac atcgcgggca aagcgcggcc
     4621 cggtataggt ggtgtagagg aagtacagat catcggcggg ctgcagccag cggtgcgccc
     4681 gccaggcaaa gaaacgcggc atggccagag cctgcagttc cgcggccgac tgctcaatta
     4741 ccaggtcgtt gattgagacc agatagctgg tctgaaagcc catctcgggg aaggccagga
     4801 agatgcggtt gaggccgatg gtatattcgc cccgcagttt ggtcaggtcg gtctggcgca
     4861 gcgaaggccc gttgcccagg atgaagcagc gctgcccggc gtgaaggttg cgcagcgcgc
     4921 ccaggcggtc gcgggtctgg cgccgccagg ggtggaatgt ggctgcgggc cagtctttca
     4981 ggcgggccag gccgtcacgg gtctcgcgca gcaggttcag cgcgggcgcc ggcagaattt
     5041 ttttcaaagt ctcacgcatg ggggcctcac tccgtgctca ggcgggcggt ggcgccgccg
     5101 tccacaatca gcgcctgccc ggtcataaag gatgactgcg tcccatctgc caggaaatag
     5161 accgcatggg cgatctcttc gggcaggccc acgcgcccgt tgacggtctt gcgcgccagg
     5221 ttgtccagcc gctcggtgat cgagccgtcg cccacatggc cgcgccccag cccggcccgc
     5281 agcatgggcg tatccaccgc accgggcagc accgcattca cgcggatctg gtcgggggca
     5341 aactcaatcg ccatggcgcg agtcagcgcc agcagcccgc ccttgctggc cgcgtaggcg
     5401 gcaatattgg ccgaggtctg caccgcgtga accgaagaca tattgacaat ggcgccgccg
     5461 ccgcccgcct tgagcagcgg gtgggccagc ttggcgccca gaaaggccga gcgcaggttc
     5521 gaggcaatcg tcaggtccca ctctgctacc gaggtttcaa cgatgggctt ggccacctgg
     5581 agcgcggcgt tgttcaccag cgcgtccagc gtgtgcgtga acgagcgggt ctgcgcgatg
     5641 atgctctcca gcgcggcggg gtcggcaata tccgcctgga tgaagaaacc atccgacggg
     5701 aacggctcgc caaacggggc gcggtccacg ccgatgacgg tccagccgtt ctgggcaaag
     5761 agctgcaccg tggcgcggcc aatgccgccc gcggcgccgg tgatgagcat aatccttttg
     5821 gggtcagaca ttgttaatct cgtttctcca tggggtgggc aggtcagggg cagagcagcg
     5881 gcacggtcag cgggtctaat ccggcgcggt actgtgcgca cagcgccggc ccggcggcag
     5941 actgccggat gaaggcagcc agttcagccg cctcggttgt acgattttcg tgcaaataac
     6001 cctgaagcat gggcagccat tccagcgggt cggcgggagc caggccctgc tggcgggcgg
     6061 catcgcccag gcgggcagct tcgatccaat cggcctgctg gcgggccagt gaggcctttt
     6121 gataataata gcaccagcca tgcgccggtt cggggccaaa aacagccggg taaaccaccg
     6181 gttcgggtgc atccaccacc acttgctggg ggtgcgaata gggcgcggtg agctgcagca
     6241 gcggatcggc agccgcggct gccagcaggt cgggctgggc ggggtccagc aggtgcacgc
     6301 agacctgcgg gtcagcccag gagacgatca gcgcccgggt aaagtcgcgc tcaaactcaa
     6361 acgtgcgcga ggagcgcagt gaaccggtga caccggcggt gaggagggcc gcggtatcgg
     6421 cgttcagcac ctcggcttca atcgccagcc ggggctcgcc cgggtgataa atgaggttgg
     6481 ccggggagaa gatttcataa tcttcagcca ggcggtaatt cggcggcaga taggccatca
     6541 ggagcgtttt gtcttcaagc tgcggggcgc gccaggccag ttgatgccag aaactgcgtt
     6601 gcacctgcca gaactggccc cagtaaattg cgttcagggt atgcgtcagc agggcaatgg
     6661 caatcagcac agccacccag ccccgcgccg ctcggatgcg ggccgcgccg aagatcaggc
     6721 cggtgagcag cagggccacg cccagggtgg cctgcagggt gtagcggtca aactggtcgc
     6781 ggaaatagac gttgcggttg gaggccagca ccggcagcag cgtgaccagt accatcaacg
     6841 cgcccagcac aatggcggcg cgcccaaaat cgtcttttac gggggcctct gccgggcgct
     6901 cctggcgggc agcaaagaac caccacagcc ccgcgcccgc agccgcggcc agcagcccgg
     6961 cggccatctg cccggccggg gcattgaccg ccagttgata cagcggcacc ccccaggcca
     7021 gaaaagttgt ttccaccagg tctttgaggg tttcgatact gagctgcccc agcatcgcca
     7081 gcggctgccc ggtgtacagc gcccacagcg cgccggcatc ggtggcgggg cgggtggaat
     7141 ggaagataaa caggcgccag tagagaaagg cgccggcagc cagcaggtag ggcaggtaga
     7201 tgagcgccgt tttgggcagc catttgcgcc agccgcagcc ctgccgccgc agcacataga
     7261 tgatgagcag caggcgcacc gcttccaggc cgatcatata ctcataaatg agcagatacc
     7321 cccccgccag caccgctccc agcgccagcg ccgccgcctg cagccagttc cgacgcgccg
     7381 ttgtcagggc aaataccgtc agcgcgatgg aaaaaatggc cagggcatag ccaatgaagt
     7441 gattttggaa cgtgttggca ttaggctgct gcaaaaaccc agggtagagg atgaacaatg
     7501 ccgccgcggc ggcagcttcc aggcggcgcg ccggccagag caggcgcagc agggcataaa
     7561 agcccaccac gctggcaaag cgggcggcaa aggcaaacaa atgccagttg agcggcgccg
     7621 agcccagcaa aataaacact ttggcgtata accgccccat gaagggccgg tcaatcataa
     7681 attcatcaat aatccgtcca gggccaaaag aactgcccgc ccagatgaca tgccagtcat
     7741 ccttataaaa acccaactgc aaaatcaagg gcagataggc cagcacagcc agcagcagaa
     7801 gcagccccag cgtgacggca tacggcagcc agtccggacg cgcccggcgg ctcacggcac
     7861 cgcctccggg gcgggcagct tatcccacag cacgtagggg gaaccggtct tgaagtagat
     7921 ggtgt
//
LOCUS       DAOPVS010000089         7890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9191_length_7890_cov_43.561200, whole genome shotgun sequence.
ACCESSION   DAOPVS010000089 DAOPVS010000000
VERSION     DAOPVS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9191_length_7890_cov_43.561200"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKL11_07780"
     CDS             complement(<1..391)
                     /locus_tag="PKL11_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNZ13541.1"
                     /translation="MPRISASQWDQFLANWPQAHLLQTSAWAALKQPFGWDADWVQIA
                     DAGAQILFRRLPLGLSVAYIPKGPVGPDWTSLWPEVDRICRRHGAIFLKVEPDAWEFE
                     VDALTPHLPGFIPAEPIQPRRTIVLDLS"
     gene            complement(395..2284)
                     /locus_tag="PKL11_07785"
     CDS             complement(395..2284)
                     /locus_tag="PKL11_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNZ13542.1"
                     /translation="MPRKPHISNRYLLLGDLFFIVVSVFASYALRLDIGPVLMFYLPS
                     ALWMIGISLVVKPLVYYTLGLYRRMWMYASMRELKLIAIAVTVAEIPVFMAMISLLSL
                     NVFRGMPRTALVIDWLLSLIFVGGLRFSVRLWSESVARAQAARQGQTRRVLIVGAGDA
                     GALVVRELQKNPQLDLEPIGFLDDNPTKLRQQIYGIPVIGTLEDLAETLERRRVDEVV
                     IAIPSAPGRVVRLVADACRGRGVPFRTIPGIYELLGGKVSVNRLREVDITDLLRRQPT
                     QLKEELMGQTLNGRIVLVTGGGGSIGRELCRQIARWGPSMLILMGHGENSVFEAVLDL
                     QENFPSLLVKPVIADVRDQPRLQTIFSQYHPQVVFHAAAHKHVPLMELNVEDAITNNV
                     MGTRNLVETALEYDVERLVMISTDKAIRPANVMGATKRLAEMLVLDAARRTGKPFSVV
                     RFGNVLGSRGSVVPKFKRQIARGGPVTITHPDMERFFMTIPEAVYLVLQAAGMSNGCE
                     TFVLNMGQPVRIQDLAEDLIRLSGLEPGRDIEITYTGIRLGEKLSEQLWEEGDILEPT
                     EHPEILRLMGQTNLQDLELQTAVDDLIELARSGDAPAIISTLDRIIPGAMIRSTPPSQ
                     IESID"
     gene            complement(2284..3714)
                     /locus_tag="PKL11_07790"
     CDS             complement(2284..3714)
                     /locus_tag="PKL11_07790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNZ13543.1"
                     /translation="MGIYTKPLKRTWRLNSGERKIILFVGDVIASVLALMIALYFWAQ
                     SPQEWLNFSWQFLGERIPGWYYLLPVIWMLLLVELYDVDRSSNRLETLKGIAIAAIIA
                     VILYLIVYFTSEPNSLPRRGVASFILGAFLLTLGWRMLYIAIFTAPRFMRRVIVVGAG
                     RAGTTLLQVIKSTWPPPFYLVGLVDDDPEKIGAEFLDYRVLGSSADLMALIKEHHVSD
                     LIFAISGEMRPETFRVLLEAEEHGVQVTTMPAVYEQLLGRVPIFLLQSDWILRSFLDQ
                     AHLSGFYEVGKRLIDLLGGFIGLLIFVIATPFIALLILLDNGRPVFYSQERVGLNGRV
                     FTILKFRTMRSAPVKPGEKAAPTVENDARITRLGKILRKSHVDELPQFINILRGEMSL
                     VGPRSEQPAIVQELQEQIPFYRARLLVKPGLTGWAQVNYGYASNAAQNAIKVEYDLYY
                     IKRRNLLLDVLILFRTFGAVFGLRGQ"
     gene            complement(3724..4725)
                     /locus_tag="PKL11_07795"
     CDS             complement(3724..4725)
                     /locus_tag="PKL11_07795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13544.1"
                     /translation="MPEVSVIVPCFNEEKTIVLLLDALYRQTQPRADLEIIIADGFST
                     DRTRTVIAEYQRAHPDLSIRVVDNPARTIPSGLNAAIAASHGAFIVRLDAHSVPASDY
                     IERCLNDLRAGLGDNVGGIWEIRPGSPTRIARAISLAAAHPFGVGDASYRLGAASGPV
                     DTVPFGAYRRSLVDKIGPYDPTLLSNEDYEFNTRIRQSGGTVYLDSLIRCIYYSRATL
                     PALARQYSRYGYWKYQMLRRYPDTLRWRQALPPIFVFGMLLLALLAPWWSLAALTFLG
                     VTLLYLLTLGVGSVPLARRHHDPALIYAIPLAIIVMHFCWGGGFLWSIISTVFRRTG"
     gene            complement(4718..6151)
                     /locus_tag="PKL11_07800"
     CDS             complement(4718..6151)
                     /locus_tag="PKL11_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ13545.1"
                     /translation="MPSLRNRLWLSYAALIGVLSLTVAVTLLWSLLRSPLLYRSTTQQ
                     LRLISALTARRLQSQPDLQSEKIYRQLDLAAQESDLRLAIINLSGQVTYDSGAEQYPA
                     LRLTDLTLSPVVQMDVPVRTFRVPQSRVWFYVLQPLPGDYILVTTTPRPQQTLRGILR
                     DEMLGPLFFSGGLALLLAFIFSAFMSRWIARPLQQLAEGARSMAKGQYPILTPSGPRE
                     IFDLTQSLNDLSQQVQTSQQAQRDLIGNISHELKTPITAIQGFSQAILDGTVTSPAAL
                     QEAATVMSVESDRMHRLVSDLLTLTRLEGGIADLHRAPVDLNALIHHAQARFSLQARQ
                     SGVELVVLSGAVPPIIGDGDRLSQVLANLVDNALKYTPPGGKITLSAAAEGDWAILRI
                     TDTGPGIPADEQERIFERFYQLDKSRRGGSGRGVGLGLPIAREIIRAHSGSLWVESSP
                     GQGSVFVIKIPFVLPDDITLNQKANNA"
     gene            complement(6154..6837)
                     /locus_tag="PKL11_07805"
     CDS             complement(6154..6837)
                     /locus_tag="PKL11_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13546.1"
                     /translation="MKSSLILLVDDEPSIVALARLYLEREGFTVQSVADGSAALESVN
                     QLQPALVVLDVMLPELDGFEVCRRLRAAGSSVAILMLTAREEDIDKILGLELGADDYM
                     TKPFNPRELVARVKSILRRIERVDYAQTGEIFRLGNLLIDPDRREVRVNGNMVEMRAQ
                     EFEVLWVLAKHCGKVLSREQLLNLAWGFDFYGQTRTVDMHIAQLRRRLANSTVHIETV
                     TGVGYKLVG"
     gene            complement(6859..7278)
                     /locus_tag="PKL11_07810"
     CDS             complement(6859..7278)
                     /locus_tag="PKL11_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNZ13547.1"
                     /translation="MSCCERKALQSDRAPKALGPYSIGVVAGHFLFTSGQIGIDPATG
                     NIVEGGIEAETRQVFANLRAVLQAGGADLKDIVKTTVFLRDIADFARMNAIYAEYFTE
                     NPPARSAFQVAALPKGAAVEIEAVAILPDTDEKCCCG"
     gene            complement(7370..>7890)
                     /locus_tag="PKL11_07815"
     CDS             complement(7370..>7890)
                     /locus_tag="PKL11_07815"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNZ13548.1"
                     /translation="LMITFHGAIFLSLKTTEPLSSDARRWASRLWLPALIALALFVGL
                     SLGLTDILAGLQVPPVLVIVLAVLAVVAMLVAGLMVRQKRDGTAFIAMVITILLSVVT
                     IFVSLFPRVLVSSLDPAFSLTIYTAASGPTTLKTMSIVALILVPVVLAYQGWSYWIFR
                     KRLSEKSEALHY"
BASE COUNT         2007 a         2054 c         2333 g         1496 t
ORIGIN      
        1 cagacaggtc taagacgatg gtgcggcgag gctgaatggg ttcggcgggg ataaagccgg
       61 gcagatgagg agtcagagca tccacctcaa attcccaggc atcgggctca actttgagga
      121 agatggcgcc gtgccggcgg cagatccggt caacttcagg ccagagtgag gtccaatcag
      181 ggccaacggg gcccttgggg atgtaggcaa cgctcaggcc caggggcaaa cgacggaaaa
      241 ggatttgtgc cccggcgtct gcgatctgaa cccaatcagc atcccagcca aagggttgtt
      301 tgagtgcagc ccaggcagag gtttgaagca ggtgagcctg gggccaattg gctaaaaatt
      361 gatcccattg cgaggctgaa attcggggca tgggctaatc aatcgattcg atctgcgatg
      421 gcggggttga acggatcata gcgccaggga taatgcggtc caacgtggaa ataatagccg
      481 gggcatcacc agagcgggcc agttcaatca ggtcatcgac ggcggtttgc aattcgagat
      541 cttgcaaatt agtttgtccc atcaagcgca agatttcggg gtgttcggtg ggctccaaga
      601 tatcaccctc ttcccagagc tgttcgctta atttctcgcc caggcgaatg ccggtatagg
      661 tgatttcgat atcccgaccg ggctcgaggc cagaaaggcg gatgagatct tctgccaggt
      721 cttgaatacg gactggctgc cccatattca agacaaaggt ctcacatcca ttactcatac
      781 cagccgcctg gagaaccaga tagacggcct caggaatggt catgaagaag cgttccatat
      841 cggggtgcgt gatggtgacg ggtccgccgc gagcaatctg gcgtttgaac ttcgggacga
      901 cgctgccacg gctgccaagg acgttgccaa agcgcacaac agagaagggt ttgccagtcc
      961 ggcgggcggc gtccagcacc agcatttcgg ccaggcgctt ggtggcgccc atgacattcg
     1021 ccggacgaat ggctttgtcg gtagagatca tcaccaggcg ctcaacatcg tattccaggg
     1081 cagtttcgac cagattgcgg gtgcccatga cattgttggt gatggcgtct tcaacattca
     1141 attccatcaa agggacgtgt ttatgggcgg cggcatgaaa gaccacctga ggatgatact
     1201 ggctgaagat agtctggaga cgcggctggt cacgcacatc ggcaatgacc ggtttgacca
     1261 gcagagaggg gaaattttct tgaaggtcaa gaacagcttc aaagacgctg ttctcgccat
     1321 gccccatcaa aatcagcatg gatgggcccc agcgtgctat ctggcggcag agttcacgtc
     1381 cgatagagcc accgccgccc gtgaccagca caattcgccc gttaagggtc tgccccatca
     1441 actcttcttt gagctgagtg ggttggcggc ggagcaagtc agtaatgtcc acttcgcgca
     1501 gacgattgac gctgactttg ccacccagga gttcgtagat gccagggatg gtgcggaatg
     1561 ggaccccgcg accacggcaa gcatcggcaa ccagacgcac cacgcgcccg ggcgccgaag
     1621 gaatggcaat gaccacctca tccacgcgcc ggcgttcaag tgtttcggca aggtcttcca
     1681 gggtaccgat gacgggaatg ccgtaaatct gctggcgcag cttggtggga ttatcatcca
     1741 ggaagccgat gggttccagg tcgagctggg ggtttttctg aagttcgcgg accaccagcg
     1801 cgccagcatc gccagcgcca acaatcagga cacggcgggt ttgaccctgg cgggcagcct
     1861 gagccctggc aacactttca gaccagaggc gaaccgaaaa ccgcaggccg ccaacaaaga
     1921 tcaaagacag aagccagtca atgaccaggg ctgtgcgtgg catgccgcga aaaacgttga
     1981 gtgaaagcaa tgaaatcatg gccatgaaaa caggaatctc ggcaacggtg acagcaatgg
     2041 caattaactt cagttcacgc atgctggcgt acatccacat acgccgatac agacccagcg
     2101 tgtagtacac cagcggcttg actaccagtg agataccgat catccacaaa gccgatggca
     2161 ggtagaacat cagcaccggg ccgatatcca ggcgtaaggc ataactggca aataccgaca
     2221 caactataaa aaacaggtcg cctaacaaaa ggtaacggtt gctaatatgc ggttttcgcg
     2281 gcactattgt cctcgcagtc caaatacagc gccgaatgtg cggaacagga tgagcacatc
     2341 cagcagcaga ttgcggcgtt tgatgtaata aagatcgtac tctactttga ttgcgttttg
     2401 agctgcgttt gaggcgtaac cataattgac ctgcgcccag ccagttagcc cgggtttgac
     2461 caataagcgg gcccgataga aggggatctg ctcttgaagt tcctgaacaa tggcgggctg
     2521 ctcagagcgc ggcccaacca ggctcatttc cccccgaagg atattaatga actggggcag
     2581 ttcatcgacg tgactcttgc gcaaaatctt gcccaggcgg gtgatgcgag catcattttc
     2641 aaccgtggga gcggcctttt caccgggctt gacgggggcg gagcgcatgg tgcggaattt
     2701 caggattgtg aaaacccgtc cattcaggcc gacccgttcc tgagaataaa agaccggacg
     2761 accattatcc agcaaaatca acagggcgat gaacggtgtg gcaatgacga agatcagcag
     2821 accaataaag ccgcccagca agtctatcag gcgcttaccg acttcataaa agccgctcag
     2881 gtgagcctga tccaaaaacg agcgcagaat ccaatcggat tgaagcaaaa agatgggcac
     2941 gcgcccgagc agctgctcat agacggcagg catggtggtg acctgaacgc catgttcttc
     3001 ggcctccaga aggactcgaa acgtttcagg gcgcatttcg cccgagatgg caaaaatcaa
     3061 gtcagagaca tggtgttctt taatcagcgc catcaaatcg gcgctggaac ccaagacgcg
     3121 ataatccaga aattcggcgc ctattttttc agggtcgtca tctaccaggc cgaccagata
     3181 aaagggcggc ggccaggtcg atttgatgac ctgtaacagg gtggtgccgg cccgcccagc
     3241 gccaacaacg atcacacggc gcatgaaacg cggggcagta aagatggcta tgtagagcat
     3301 gcgccagccc agggtgagca gaaaagcgcc caggataaag ctggccacac cgcgacgggg
     3361 cagtgaatta ggctcagacg tgaaatagac gatcaggtaa agaattacag cgataatcgc
     3421 ggcaatggca atgcctttga gcgtctcaag acggttgctg gagcgatcca catcatacaa
     3481 ttcgacgagc agaagcatcc agatgacagg caataaataa taccagccgg ggatgcgttc
     3541 tccgagaaac tgccaggaaa agttaagcca ttcctgggga gactgggccc agaaataaag
     3601 tgcaatcatc aaggccagga cagatgcaat cacatctcca acaaagagga tgatcttgcg
     3661 ctcgccagaa ttcaaccgcc aggtacgttt taagggtttg gtataaattc ccatagctcc
     3721 attttaaccc gttctgcgaa agacggttga aattatactc cacaaaaagc caccacccca
     3781 gcaaaaatgc atgacgatga ttgccagggg aatggcataa atcagcgctg gatcatgatg
     3841 gcgcctggca agcggcacgc tgcccacacc aagagtgagg agatataaga gcgttacccc
     3901 taaaaaggtc agtgctgcca gggaccacca gggggccagg agggccaaga gcaacatacc
     3961 gaagacaaat atcgggggga gcgcctgacg ccagcgtaaa gtgtcgggat aacgccgaag
     4021 catttgatac ttccagtagc catagcggct gtactgccgc gccagagccg gcagcgtggc
     4081 ccgagaataa tagatacagc gaatcagcga atcgagatag acggtgccgc cagactgccg
     4141 aatgcgggtg ttaaattcat aatcctcgtt gctcaaaagg gttgggtcgt aggggccaat
     4201 tttatctacc agagaacggc ggtaagcgcc aaacgggacg gtatcaaccg ggccagaggc
     4261 ggcccccagc cggtaagagg catcgccaac gccgaagggg tgggcggcgg ccagtgagat
     4321 ggcacgagca atgcgtgtgg ggctgccggg tcgaatttcc cagatgccgc caacattatc
     4381 ccccagacct gccctaaggt cgttgagaca gcgttcgata taatctgatg ccgggaccga
     4441 atgggcatcc aggcggacaa taaacgctcc atgagatgcg gcaatggcgg cgttcaggcc
     4501 agacgggatc gtccgagcgg ggttatccac cacccggatg gacaggtcgg ggtgagctcg
     4561 ctgatattca gctatcaccg tgcgggttcg atcggtagag aagccatcag caataatgat
     4621 ctccaggtct gcgcgaggct gggtctggcg gtaaagcgca tccagcagta acacgatggt
     4681 cttttcttca ttaaaacaag ggactatcac agagacttca ggcattattg gccttttgat
     4741 tcagggttat atcgtctgga aggacgaagg ggattttgat gacaaaaacg ctgccctggc
     4801 ctggagatga ttctacccag agggagccgg aatgagcgcg aataatttcg cgggcaatgg
     4861 gcagacccag cccaaccccc cgccccgaac cgccgcggcg ggatttatcg agctgataaa
     4921 aacgttcaaa gatgcgttcc tgttcgtcgg cggggatgcc ggggcctgta tcggtaatcc
     4981 gcaatatcgc ccagtcgcct tctgcggcag cgctgagggt aattttaccc ccaggagggg
     5041 tgtatttgag cgcattatct accagattag ccaggacttg cgaaaggcgg tcgccatcgc
     5101 cgatgattgg cggcacggcg cctgagagga caactaattc aacgcccgac tggcgagctt
     5161 gcagggagaa gcgcgcctga gcatggtgga tcagggcgtt caaatctacg ggggcgcggt
     5221 gcaagtctgc gataccaccc tccaggcgcg tgagggtgag caggtcagag accagccgat
     5281 gcatccggtc tgattcaacg ctcatcacgg tggcggcttc ctgcagggca gcgggagagg
     5341 ttacggtacc atccaaaatg gcctgcgaaa agccttgaat ggcggtgatg ggggttttta
     5401 attcatgtga aatgttgcca atcaggtcgc gctgggcctg ttgtgaggtt tgcacctgtt
     5461 ggctcagatc gttcaaggat tgggtgagat caaatatttc gcgcgggccc gagggggtga
     5521 gaatggggta ctgaccttta gccattgacc gggcgccttc ggcaagctgt tgaaggggtc
     5581 gagcgatcca gcgcgacatg aaggcgctga agatgaaggc cagcagcagt gccaaaccgc
     5641 ccgaaaagaa caatggacct aacatttcat ctcgcaggat gccgcgcaag gtttgctgtg
     5701 ggcgcggcgt tgttgtgacc aggatataat caccgggcag cggttgtagt acgtaaaacc
     5761 agactcggga ttgtgggacg cgaaatgtgc ggacggggac atccatctgg acgaccgggg
     5821 acagggtgag gtcggtaagc cgcaatgcag ggtactgttc agccccactg tcataggtca
     5881 cctgccctga gagattgata atcgccaacc gcaggtcaga ttcttgagcc gcaagatcta
     5941 gctggcggta tattttttcg gactgaaggt cgggctggga ttgcaaccgc cgggcagtca
     6001 gtgcagatat aagccgaagc tgctgggttg tgctgcggta caacaatgga ctgcgtaaaa
     6061 gcgaccagag caatgtgaca gcaacggtca gcgacaggac accaatcaga gccgcatagc
     6121 tcagccagag acgattacgc agggagggca tggttaccca accaatttgt atccgacgcc
     6181 ggtcaccgtt tcgatgtgaa ctgtgctgtt agccagacgg cggcgaagct gggcaatgtg
     6241 catatcaacg gtgcgcgttt gaccgtaaaa gtcaaacccc caggccagat tgagaagttg
     6301 ttcacggctt aagactttgc cgcaatgttt cgccagaacc caaaggacct caaattcctg
     6361 ggcgcgcatt tcaaccatgt tgccattgac acgcacctcg cgccggtcgg ggtcaatgag
     6421 caggttgccc agacggaata tctcgcccgt ctgggcataa tcgacgcgct caattcgccg
     6481 gaggatggat ttgacccggg caaccagctc acgcggattg aagggcttgg tcatgtagtc
     6541 gtcggcgccg agttctagcc ccaggatttt gtcgatatct tcctcgcggg cggtcagcat
     6601 tagaattgcc acagaactac cggctgcgcg cagacggcgg cagacctcaa agccatccaa
     6661 ctcgggcagc atcacatcca gcacaaccag cgctggctgg agctgattga ctgactccaa
     6721 tgcggcactg ccatctgcca ccgattgcac agtgaagccc tcgcgctcca gatagaggcg
     6781 agccagggcg acaatactcg gttcgtcatc caccagcaaa atcagagaag atttcatgga
     6841 ttatgaaaag cggagggcct atccgcagca gcacttttcg tcagtatcgg gtaagatggc
     6901 aaccgcctca atctctacgg ctgcaccctt gggcaatgca gccacctgaa aagcggagcg
     6961 ggcaggggga ttctcggtaa agtattcagc ataaatggca ttcatacgtg caaagtcagc
     7021 aatatctcgc aggaagactg tggttttgac aatatcctta agatctgcgc cgccagcctg
     7081 gagcacagcc cgcaaattgg caaatacctg acgggtttca gcctcaatgc cgccctcgac
     7141 aatattgccg gtggcaggat caatgccgat ttgcccggat gtgaaaagga agtgcccggc
     7201 aacaacgcca atcgaatatg ggccaagggc tttaggtgca cgatcggatt gtagggcttt
     7261 gcgttcacag cagctcatga tgtcctccat aacaagaatg ttgcgtttaa agtatctgag
     7321 tgcgaactgc cgtgaagcaa tccgcactca gaattatacc ctacccggat taatagtgaa
     7381 gggcttcgga cttttcggat aggcgcttgc ggaagatcca gtaagaccag ccctggtaag
     7441 ccaggacgac tggcaccaga atgagcgcaa caatgctcat ggtcttgagc gtagtagggc
     7501 cagaagcggc ggtgtagatt gtcaggctga aggccggatc gaggctggag accagcacgc
     7561 gcgggaagag cgagacaaag attgtgacaa ccgagagcaa aatggtgatg accattgcga
     7621 tgaaggcggt accatcacgc ttctgccgca ccatcaggcc ggccaccagc atggcgacca
     7681 ctgccagcac agccagcaca atcaccagca caggcggaac ctgcaagcca gcgaggatgt
     7741 ccgttagacc gagtgacaga cccacaaaca acgccagggc aatcaatgca gggagccaca
     7801 ggcgggatgc ccagcgccgg gcgtcagaag aaagaggctc ggtggtttta agcgaaagga
     7861 agatggcgcc atggaatgta atcatcagca
//
LOCUS       DAOPVS010000090         7802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9320_length_7802_cov_51.450497, whole genome shotgun sequence.
ACCESSION   DAOPVS010000090 DAOPVS010000000
VERSION     DAOPVS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9320_length_7802_cov_51.450497"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PKL11_07820"
     CDS             <1..629
                     /locus_tag="PKL11_07820"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNZ13549.1"
                     /translation="QATLVFGEDVQIDGFEIDPKIVEVGRTYFDMNEPNLNVIVQDGR
                     WGLAHSTNQYQIISVDAYRPPYIPWHLTTQEFFQVVNDHLTDDGVMVINVGRAGTDRR
                     LIDALSTTILTVFPTVHVVDVPNSFNTIVFASKQPTEEYNLGQNYLALVEQGAPDLLI
                     QTLEIAITNLQPTPETTIVFTDDKASVEWITNDMVMSYLLSGESETLK"
     gene            626..1285
                     /locus_tag="PKL11_07825"
     CDS             626..1285
                     /locus_tag="PKL11_07825"
                     /inference="COORDINATES: protein motif:HMM:NF018964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNZ13550.1"
                     /translation="MKRVGLFLISLLVPFILLMAAVRIAFTPFFLVMEYRAPNFPPDN
                     YGFTTEERLYWGKISLKYLLDGSDISFLGEQRLPNDEPLYNERELSHMVDVKVVLQKT
                     TLAWGILCAVVAAIGIWSWRGKWLSDFVRALATGGWITLGLIGAILVGVAVSFNQLFG
                     AFHTLFFTSGTWVFEYSDTLIRLFPIKLWQDAFIFVGVVTAALALAAALVLPKIKSRK
                     A"
     gene            complement(1354..2103)
                     /gene="gpmA"
                     /locus_tag="PKL11_07830"
     CDS             complement(1354..2103)
                     /gene="gpmA"
                     /locus_tag="PKL11_07830"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNZ13551.1"
                     /translation="MYKIVLLRHGESDWNRENRFTGWTDVDLSEKGLAEATQAGELLK
                     EAGFTFDVAYTSVLKRAIRTLWIVLDRMDLMWIPVHRSWRLNERHYGALQGLNKAETA
                     EKFGEEQVKLWRRSYDVPPPALTADDPRYPGKDPRYADVGPIYLPLSECLKDTVARFL
                     PYWFETIVPAVKTGRKVLIVAHGNSLRSLVKYLDDVSDEEIIGLNIPTGIPLVYELDE
                     QMKPIRHYYLGNPDDVAAAMQAVANQAKKKS"
     gene            complement(2182..3294)
                     /gene="ugpC"
                     /locus_tag="PKL11_07835"
     CDS             complement(2182..3294)
                     /gene="ugpC"
                     /locus_tag="PKL11_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNZ13552.1"
                     /translation="MASVTYDHVWKKFGDFTALNDLNIEIKDKEFLVLVGPSGCGKTT
                     ALRCLAGLEEVSSGKILIGDRVVNDVPPKDRDIAMVFQSYALYPHMTVYDNMAFGLKL
                     RKMSKEDINRRVLEAAGVLGIEHLLKRRPRELSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQTRAEISKLHLRLQTTFIYVTHDQTEAMTMASRIAVMNKGILQQLDTP
                     QTLYDFPANQFVAGFIGSPSMNFFSAKLRKDGEKLYVDGDGFAVEIPAPRVAPYMSYV
                     GKDVIFGIRPEDIFNPQFIPPGIHTAPVEAKVDVTELMGNEIFLYLVSGNSNYVARVD
                     PRTRFTIGEKVQVAFNMDKFHIFDASGNAENPVAVR"
     gene            3466..4137
                     /locus_tag="PKL11_07840"
     CDS             3466..4137
                     /locus_tag="PKL11_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13553.1"
                     /translation="MMDKNMILALIPGYNEAQRIAAVVNTAGTHLPVLVVDDGSSDNT
                     RGVAEAAGAQVIRQEPNQGKGAALRAGFRWALANGYEAVITLDADGQHDPAEIPGFLD
                     AYAQDKSDLIIGARDFSQMPAVRRWANTTGQKLFSWALGQPVQDNQSGYRLISRRLME
                     AVLESAESGFEFEVEMIVVCVRGGMKLDWVPIRTIYGDQGSHIKPLHHAAHFMRIIWK
                     TWRRR"
     gene            complement(4134..4592)
                     /locus_tag="PKL11_07845"
     CDS             complement(4134..4592)
                     /locus_tag="PKL11_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13554.1"
                     /translation="MENQHTHALAVYQACEDFLALGPEPHASAAMVAADLQYSREHGG
                     IYSGIWHLETLVGVLDYIPSHYEGQPHCAFINLLMIIPSWRGSGLGHSVIAWLEQQLS
                     ARGIIQIWIAIQVNNPLALSFWQHCGYAIKTSPARQSDGTTTQLLAKSLV"
     gene            4680..5195
                     /locus_tag="PKL11_07850"
     CDS             4680..5195
                     /locus_tag="PKL11_07850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ13555.1"
                     /translation="MPTNLSWPKPPELEIDLKKSYTATFHTDKGDIVIKLHADRVPRT
                     VNNFVFLARQGFYNDTIFHRVINNFMAQGGDPTGTGMGGPGYKFNDEFDPKLRHDKPG
                     VLSMANAGANTNGSQFFITHVPTPWLDGKHSVFGQVIQGMDVLMSIPARDPQRVGAPA
                     VKLISVTITEE"
     gene            complement(5275..>7802)
                     /locus_tag="PKL11_07855"
     CDS             complement(5275..>7802)
                     /locus_tag="PKL11_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ13556.1"
                     /translation="LAWRYLSGHKLRTTLTTLSIVLGVMIIFGLNSMIPAMERIVARQ
                     MMAAVSEVELSVRSVSGSPFDSQTLAVVQSAKGVQSATGILSQLIAIPEGLVRPAVDE
                     YQVVRTVMLIGVNPDTIAAVRAFSISSGRFLEPGDTTAVVLPVSLSQKLGLQPGDIWT
                     LPSATGTVDLTVVGIFKPSMPTLGTEEIYAPLAQVQSILNLPDQISVVEAVLDANADK
                     TAVSSAIQTELGSTFTLIAPDGDSEFSSAVQVGQAAFLLFGILSLAMGGFVIFNTFRT
                     VVAERRRDIGLLRAVGASRRTIVLSILTETLLQGLMGSALGLLLGYLFAVVLMMIVTP
                     LAQKFLYMDMGAPIITLGNFLVALGLGLGITVLAGLIPALSASKIPPIEALRLESVGQ
                     VARKVRTRALIGFVMIALAVIALITGSFNLAALGAILFLVGVILAAPVLVRPLAETFG
                     RLLNLFFAREGHLAQRNLARQPERAAVTALAMLIGLSIVVALAGVISSTFGGFLSYLN
                     RSLGADYLVMPTSLVLGGGNMGAAPDLAAELSQVEGVAGVTTLRLASGKVDGTNLQVI
                     GIDPQTYPVIAGLEFSRGESAEAYAALDQERAMIVNGIFAATTGAQIGDLVTIMTPSG
                     EQQYRIVGVGLDYLNAKLTTGYISQANLERDFLTTTDVLLMANAVDGADKSTVGARLT
                     NLISAYPAFTLLESDTFYQAQVNIFKAALAMMYVMMAAMALPALIAMMNTLAINVIER
                     TREIGVLRAVGSTRKQIRRMILAESLLLALFGTGLGILAGVWLSYVLVGAMNFSGFVM
                     QFNFPWGGVLAGLAVGLIFGVLAATLPARQAARLDIIHALRFE"
BASE COUNT         1886 a         1958 c         2324 g         1634 t
ORIGIN      
        1 ggcaggccac actggtattt ggcgaggatg tgcagatcga tggatttgaa atcgatccga
       61 agattgtcga agtggggcgc acctactttg acatgaatga gcccaacctg aatgtgattg
      121 tgcaggatgg ccgctggggg ctggcacact ctaccaacca gtatcagatt atcagcgtgg
      181 atgcttaccg tccgccttat atcccctggc acctgaccac acaggaattc ttccaggtcg
      241 tgaatgatca cctgaccgac gacggggtga tggttattaa tgtagggcgg gcagggactg
      301 accggcggtt gattgatgcg ctcagcacga ccatattgac ggtctttccg acggtgcatg
      361 tggtggatgt gcccaattct ttcaatacga ttgtgtttgc atccaaacaa cccacagaag
      421 aatacaacct gggacagaat tatctggcac tggtagagca gggtgcgcca gacttgctga
      481 ttcaaaccct ggaaattgcc atcactaacc tgcagcccac gcctgaaaca acgatcgttt
      541 ttaccgatga caaggcctcg gttgaatgga tcaccaacga catggtgatg agttacctgc
      601 tgtcgggaga atcggagact ttgaaatgaa acgtgttggg cttttcttaa tcagcctgct
      661 ggtgccgttt atcctgttga tggcggcagt acggattgca ttcaccccct tcttcctggt
      721 gatggaatac cgggcgccaa actttccacc ggataattat ggttttacaa ccgaagagcg
      781 gttgtactgg ggcaaaattt cgttgaaata tttgctggat ggctctgaca tctcgttcct
      841 gggagagcaa cggctgccga atgacgagcc gctctacaac gagcgcgagc tgtctcacat
      901 ggttgatgtg aaggtggtgc tgcagaaaac caccctggcc tgggggattc tgtgtgcggt
      961 tgtggcggca attggcatct ggagctggcg cggcaaatgg ttgagcgatt ttgtgcgggc
     1021 gctggcgacc ggcggatgga ttacgctggg gttgattggc gccattctgg tcggcgtggc
     1081 ggtgagcttt aatcaattat ttggggcgtt tcacacgctg ttttttacca gcggcacgtg
     1141 ggtgtttgaa tattcagaca ccttaatccg gctgttcccg attaaattgt ggcaggatgc
     1201 atttattttt gtgggggttg tcacggcggc gctggccctg gcggcggcgc tggttttacc
     1261 aaagatcaag agccgaaagg cttgagcggg ttgttataaa gaaaaaaccg gtgaatgtca
     1321 aaacattcac cggttttcgt tttgagaaaa gcctcaggat ttctttttgg cctggttggc
     1381 aacagcctgc atagccgcag ccacatcatc cgggttgccg aggtaataat ggcggatggg
     1441 cttcatctgc tcatccagtt catagaccag cggaataccc gtggggatat tcaggccgat
     1501 gatttcttcg tcagaaacat catccagata tttgaccagg gagcgaaggc tgttgccgtg
     1561 ggcaacaatc aacactttgc ggccggtttt gactgccggg acaatggttt caaaccagta
     1621 gggcaggaag cgcgccacgg tgtctttgag gcactcgctg aggggcaggt agatggggcc
     1681 gacatcggcg tagcgtgggt ctttgccggg gtagcggggg tcatcagcgg tcagggcggg
     1741 cggtgggaca tcataactgc gccgccagag cttgacctgc tcttcgccaa atttttcggc
     1801 ggtttcggct ttgttcaggc cttgaagcgc gccatagtgg cgctcgttga gccgccagga
     1861 gcggtgaacc ggaatccaca tcaggtccat gcgatccaga acgatccaca gagtgcggat
     1921 ggcgcgcttg aggaccgagg tgtaagcgac atcaaaggtg aaaccggctt ctttgagtag
     1981 ttcacctgcc tgggtggctt cggcaagacc tttttcggaa agatccacat ccgtccagcc
     2041 ggtgaagcga ttttcgcggt tccagtctga ctcgccatgc cgcagtaaga cgattttata
     2101 catgcactac ctccaagact taaattgtag cgcaagagaa gctcttgcgc tacatgggtt
     2161 ggtttgaatt agagagccgg gttaccgaac ggcaacgggg ttctcggcat tgccagaagc
     2221 atcgaagatg tggaatttgt ccatgttgaa ggcgacctgg actttttcac cgatggtgaa
     2281 gcgagtgcgc ggatcaacac gagccacgta gttgctgttg ccagaaacca ggtagaggaa
     2341 gatttcgtta cccatcagtt cggtgacatc cactttggct tcaaccgggg cggtgtggat
     2401 gcccgggggt atgaactggg gattgaaaat atcctcgggg cggatgccaa agatgacatc
     2461 tttgccaacg taggacatat agggagccac gcggggtgcg ggaatttcaa cggcaaagcc
     2521 atcgccatcc acatagagtt tttcgccatc tttgcgcagc ttggcagaga agaagttcat
     2581 cgagggagag ccgatgaagc cagcgacaaa ctggttggcg gggaaatcat acagggtctg
     2641 gggggtatcc aactgctgca aaataccttt gttcatgaca gcgatgcggg aagccatggt
     2701 catggcttcg gtctggtcgt gggtcacata aatgaaggtg gtttgcaggc ggaggtgcag
     2761 cttgctgatt tcggcgcggg tctgcacgcg gagcttggca tccaggttcg agagcggttc
     2821 atcgaagagg aagacctttg gctcgcggac aatggcgcgg ccaacagcca cgcgctggcg
     2881 ctgaccacca gagagctcgc ggggtctgcg cttgagcaaa tgttcgatgc ccagcacacc
     2941 ggcagcctcg agtacgcggc ggttgatgtc ttctttggac atcttgcgga gcttgaggcc
     3001 aaaagccata ttgtcataca cggtcatgtg cgggtaaagc gcgtacgact ggaacaccat
     3061 ggcaatgtcg cgatctttgg gcggcacgtc attgaccacg cggtcgccaa tcaggatttt
     3121 gcccgaggaa acttcctcca agccagccag acagcgcagc gcagtggttt taccgcagcc
     3181 cgaagggccg accaaaacca ggaattcttt gtccttaatt tcaatattaa ggtcgttcag
     3241 ggcggtgaag tccccaaact ttttccagac atgatcataa gtaacgctag ccatacagtc
     3301 ctccttccag agcgaatata gtgatttgat tatatgcccc aataaaagaa aatgcaacag
     3361 tatttgtcaa gggatttaaa aattagatta aagaaaaaca aggaatcgct gaacgtgtat
     3421 caattgcggg attgggtctg aggataagca agtataataa cgaacatgat ggacaaaaac
     3481 atgattctgg cattgattcc gggatataac gaggcacagc gcatcgcggc agtggtgaac
     3541 actgccggca ctcacctgcc ggtgctggtg gtagatgatg gatcgagtga taacacccgg
     3601 ggggttgctg aagccgccgg ggcacaggtg attcgacaag agcccaacca gggtaagggg
     3661 gctgcactgc gggcgggctt cagatgggcg ctggcgaatg ggtatgaggc agttattacc
     3721 ctggacgccg atgggcagca tgatccggcg gagattccag gttttttgga tgcctatgcg
     3781 caagataagt ccgatttaat tatcggggcg cgggattttt cgcaaatgcc agccgtgcgg
     3841 cggtgggcca acaccaccgg gcagaagctg ttttcgtggg cgctaggaca gcctgtgcag
     3901 gataatcaat cgggataccg gttgatcagc cgcagattga tggaagcggt gctggagagc
     3961 gctgaaagcg gttttgaatt tgaagtggaa atgattgtgg tttgtgtgcg gggcgggatg
     4021 aagctggact gggtgcccat ccgcactatt tacggggacc agggcagcca cattaaaccg
     4081 ctgcatcatg cggcgcactt catgcgcatc atctggaaga cctggcggag acgctagacg
     4141 agagactttg ccagaagctg tgtggtggtg ccatcagact gacgtgcggg tgaggtcttg
     4201 attgcatagc cgcaatgctg ccaaaaagac aacgctaaag ggttgttcac ctggatggca
     4261 atccagatct ggattattcc ccgtgcagat aattgctgct ccagccaggc gatcactgaa
     4321 tgcccaagcc cactcccccg ccaggaaggg atgatcatca aaaggttgat aaaggcgcag
     4381 tgcggctggc cttcataatg cgatgggatg taatccagca caccaaccag ggtttctaaa
     4441 tgccagatgc cggaatagat gccgccatgc tcgcgagaat actgcagatc tgcggctacc
     4501 atggcggccg aggcgtgcgg ctcggggccc agggcaagaa aatcctcaca ggcctggtag
     4561 actgccaggg cgtgggtgtg ttgattttcc aaaagtggtg atagaatcca ttgcatcaac
     4621 tggattaaac cacattttga tccaggtcac cttccaaaat taactcgaaa ggatctgcaa
     4681 tgcctacgaa tctatcctgg cccaagccac ccgaactgga aattgacctc aaaaaatctt
     4741 ataccgccac attccataca gacaagggcg atattgtcat caagctgcat gccgaccgcg
     4801 tgccccgaac agtaaataat tttgtgttcc tggcccgaca gggtttttat aacgacacga
     4861 tcttccaccg cgtgattaac aattttatgg cccagggcgg cgaccccacc ggtaccggta
     4921 tgggcggccc tggctacaag ttcaacgatg agtttgaccc caaactgcgg cacgataagc
     4981 caggtgtgct ttcaatggcc aatgccggag cgaacaccaa tggttcgcag ttcttcatca
     5041 cgcatgtgcc cacaccctgg ctggatggga aacacagcgt gtttgggcag gtcattcagg
     5101 gcatggatgt gctgatgtcg attccagcac gcgatccgca acgggttggg gcgccagccg
     5161 tgaagctgat ttcggtgacg atcaccgaag aataattgct tctgacaagt aaaatgaacg
     5221 aggcggagca caatgctccg cctcgttttt tcaccagaat ggacaggggc aatatcactc
     5281 aaagcgcagg gcgtggatga tatccaggcg ggcggcctga cgggcaggca gcgtggcagc
     5341 cagcacgcca aaaatcaaac caacggcaag gccggccagc acgcctcccc aggggaagtt
     5401 gaactgcatg acaaagccgc tgaagttcat ggcgccgacc aacacatagc tgagccaaac
     5461 gccggccagg atgccaaggc ctgtgccgaa caaagccagc aggaggctct cagccaaaat
     5521 catccggcgg atttgtttac gggtactgcc cacggcacgc agcacaccaa tctcgcgggt
     5581 gcgctcaatc acgttgatcg ccagggtatt catcatggca atcagggcgg gcagcgccat
     5641 ggcagccatc attacataca tcatcgccag ggcggccttg aagatattca cctgagcctg
     5701 atagaaggta tcagattcga ggagcgtaaa agcagggtag gcagagatca ggttggtgag
     5761 gcgagcgcca acagttgatt tatcggcccc gtcaacggca ttggccatca gaaggacatc
     5821 ggtggtggtg agaaaatcac gctccaggtt tgcctgggag atgtagccgg tggtcagttt
     5881 ggcgttgagg tagtcgaggc cgactccgac aatgcggtat tgctgctcgc ctgatggtgt
     5941 cataatcgtc accaggtcgc caatctgggc cccggttgtg gccgcaaaga tgccattgac
     6001 aatcatggcg cgttcctgat ccagggcggc gtaagcctca gccgattcac cgcggctgaa
     6061 ttccaaccca gcaatgaccg ggtaggtttg tggatcaatg ccgattacct gcagattggt
     6121 tccatccact ttgccagagg cgagacggag cgtagtgacc ccggcgacgc cttcgacctg
     6181 actcaattca gccgccaggt ctggagccgc acccatgttc cccccgccca gcacgagcga
     6241 ggtgggcatg accaggtaat cagccccgag gctgcgattc agatagctca aaaagccgcc
     6301 aaaggtgctg gagatgaccc cggcgagggc aaccacaatt gacaggccaa tgagcatggc
     6361 cagggccgtc accgcggcac gttcaggctg gcgggcaagg ttgcgctggg caagatgccc
     6421 ttcgcgcgca aagaagaggt tcagcaagcg gccaaaggtt tcagcgagcg ggcgcaccag
     6481 gaccggtgct gctaatatga cgcctaccag gaagagaata gcacccaacg ccgcaaggtt
     6541 aaagctgccc gtaatcaggg caatcacggc aagcgcgatc atcacaaagc cgatgagggc
     6601 gcgggtgcgg actttccggg cgacctgtcc taccgattcg aggcgcaggg cttcaatggg
     6661 cgggattttg ctggctgaaa gcgccgggat aaggcccgcc agaacagtaa tgcccagccc
     6721 caggcccagt gcaaccagga agtttcccag ggtgatgatg ggagcgccca tatccatata
     6781 caggaatttt tgcgcaagcg gtgtgacgat catcatcagg accacagcaa aaaggtatcc
     6841 gagcagcaga cccagagcgg accccatgag accctggagg agggtttcgg tgaggatgga
     6901 gagaacaatc gtccggcggg acgcgccaac agcccggagc aggccaatat cacggcggcg
     6961 ttcagcaacc acggtgcgga atgtgttgaa gatgacaaat ccgcccatgg ccagcgaaag
     7021 gatgccaaac agtaaaaacg cggcctggcc tacctgaaca gccgaggaga attcagagtc
     7081 gccatcgggc gcgatgagcg tgaaggttga acccaactcg gtttgaatgg cgctgctgac
     7141 ggcagtttta tcggcattgg catccaaaac ggcttcaacc acgctaatct ggtcaggcaa
     7201 attcaggata gattgaacct gagccagcgg ggcatagatt tcttcggtgc ccagggtggg
     7261 catggaaggt ttgaatatgc caacaacggt caaatcaact gtgccggtgg cggaaggcaa
     7321 agtccagata tctccaggct gcaagcccag cttttgagat aaagaaacgg gcaggacaac
     7381 ggcggtggta tcaccgggtt caaggaagcg ccccgacgag attgaaaaag cgcgcactgc
     7441 agcaatcgta tcagggttca cgccaatcag catgaccgtt ctgacaacct gatattcatc
     7501 tacagcaggg cggacaagac cttcgggaat ggcaatcaac tggctgagga ttcctgtggc
     7561 ggattgaacg cctttggcag actgcacaac agccaaagtc tggctgtcaa acgggctgcc
     7621 cgaaacactg cggacgctca attccacttc gctgacagcg gccatcatct ggcgcgcaac
     7681 aatccgctcc atagcgggga tcatagaatt gaggccaaag ataatcatca cacccagcac
     7741 aatggagagg gttgtgaggg tggtgcggag cttatgcccc gatagataac gccaggctaa
     7801 ct
//
LOCUS       DAOPVS010000091         7683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9516_length_7683_cov_72.151940, whole genome shotgun sequence.
ACCESSION   DAOPVS010000091 DAOPVS010000000
VERSION     DAOPVS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9516_length_7683_cov_72.151940"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..1587
                     /locus_tag="PKL11_07860"
     CDS             370..1587
                     /locus_tag="PKL11_07860"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNZ13557.1"
                     /translation="MPHIDSTPKRYIGFDIHKHYFVAIGVDPQLNPVFGPHQASITQL
                     AEWARKNLTPQDAVVVEMTTNTYLFFDIIKPLVHSVTVVHPPHVSLIVRAQVKTDKKA
                     ALTLAQLHASGLLPGIWIPPVEVRELRALVAHRNKMVHFSSIAKCRLHALLQRKGIEA
                     PQGVEPFSPEMRLWWDELDLTALEKHCMHSDLSMLDFARSQVESDEQCLKHLAGKDER
                     LPLLIQICGIGFITAITILAAIGEIQRFPTPGQLVGYAGLGARVHDSGMSRTTGRITK
                     TGRRDLRRAMVDAANHAIPFHPHWRAVFERQSMHIGRQKTIVAIARKLLVAVWHVLSH
                     QEADRFADHQQVATALFAYAHRIRVQNLPDEQSALQFTRNQLDRLKIGQEVRQIPWGS
                     RKFKLPESKLTVS"
     gene            complement(1750..2070)
                     /locus_tag="PKL11_07865"
     CDS             complement(1750..2070)
                     /locus_tag="PKL11_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13558.1"
                     /translation="MNGRLGLASLLWVVGFNLCVALIAVYWGIGWSIAILCLRHILLG
                     IPKVYDRWRGLFLTSTTSTTFVKANQQWPTRKWIIETGLLIIATIIVIVRYNIPLKNV
                     LFGN"
     gene            complement(2070..2729)
                     /locus_tag="PKL11_07870"
     CDS             complement(2070..2729)
                     /locus_tag="PKL11_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13559.1"
                     /translation="MAWDRYGYGYNNPSRYTDPTGHFNKDEIKNYFGVDSWDDVLRVF
                     QEGGTLEGRWGWLTVLQKAELGDEININWDTDQLPNNHPDVESSFTGKFTLDQAGRLI
                     IAGESSYMDQVKAAKYGSTYSLIHYGSDDAKKVAAAFLIAATDLTVGIPAFGLMLTGQ
                     PVLMKAGEALETLVVLPVNIFGVKMWQDAEHGKIEEVLNISAIPNQNIPSMPNHYPEV
                     N"
     gene            2877..3764
                     /locus_tag="PKL11_07875"
     CDS             2877..3764
                     /locus_tag="PKL11_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ13560.1"
                     /translation="MLSEIDQFVNWVRRRSPNARTWKDYSYDLHQFASFIGDLPATSV
                     TFRDVDCFVAHQVSRGFQPTTVNRRLACLVSFFTFLSDEDPSLVCPVLPKRHFLRERE
                     RLPRPVQPEQLKAFFIAIDHAIQSGCSEALRDRAMFLLMLRCGLRIGEVANMLLNGLY
                     LDEPRPRLVVSGKGSRERVVYISPQAERALRRYLALRPDSLSENVFLAYHRDGLSTTA
                     IHKRLMHYRQEAQVSLTAHRLRHSFANDLLTADVPITTIQKLMGHRWLETTQIYVQAN
                     DRQVAADYYAACARLEDWS"
     gene            3761..5359
                     /locus_tag="PKL11_07880"
     CDS             3761..5359
                     /locus_tag="PKL11_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNZ13561.1"
                     /translation="MSPAKAAVTPCEIYNHALKYSRHDHVPPGVPLPNSTNTWLPENI
                     AILERYVTWLAGGGTSAYVIRVIHIPMAGHILSLNHKPSTALDLDNDLQAGMDYILAK
                     GLGFCWTKNCRLSMLKFRRFLLHERGQTECHITPFDPVLHTEGLPAWLVEQLTRYQHV
                     KQRNWRESRIEDGIRRFWCEHLRLWRFLVRRFGIQALSDLKRSHFLDYTDWRLQAGMS
                     VRGVNGDLRTFHGFMEFLQGQDWPVPHILLRLPCLKEPDSLPKFLTDTQVCSLRDDLE
                     ARVAGADNAAHCRDALLDRATFYLLWQGGLRRGEVEDLRLEDLDLEGRKLTVRRGKGL
                     VDRTVFLSQSVVRVLLAYLPMRGPGPTDHVFLYRNQAISKDLIHGRLKACGVRVGVSV
                     YAHRLRHTCATQLLNAGCPVTSIQKFLGHKRLNTTMVYARAYDQTVEADYFAAMSRVE
                     QRLLVAPETEPTIAVVTDEKKMQILAIAGQLAAPEAEPEMRRNLFEQMRYLLFGGQTF
                     PEAVLPVEAGSQVWIPPPVDVAVG"
     gene            complement(5449..5916)
                     /locus_tag="PKL11_07885"
     CDS             complement(5449..5916)
                     /locus_tag="PKL11_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13562.1"
                     /translation="MSIRHLFKLALLILLVCGLSACDNINAETEQPKINSLQDRTAIP
                     TQTLTPHPITIGKGELMVVVEAVEKYYADHGVYPDLITDLIPGYIAQLPYTFDGHEIW
                     YERNEYQTYEVGFDPSERKYCVYLKKDNYLECGFRNPELFITPGSPTLTPPPH"
     gene            complement(5913..7121)
                     /locus_tag="PKL11_07890"
     CDS             complement(5913..7121)
                     /locus_tag="PKL11_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNZ13563.1"
                     /translation="MSSAELSTDWTQVTKTITSPLDAVTLKLELSGTQFSGWLAWDDA
                     SVSLEEQVITKYYYAGSQRVAVQNGMLAPPNYLLGDHLGSTSVTTNSAGNLVAEMLYK
                     PFGEVRYTSGTTPTQYTYTGQYSNMAEIGLMYYNTRWYDPYLNRWVQPDSIIPDPGNP
                     QDWNRYSYVRDNPLKYTDPTGHWACGDYYDTGCVETFAEAGAMMRSTGMNALAGGKTT
                     LPIVARKDWGQYAPGDNPGNFSDEDRETYDPNNEFGGYYEYPGNLADTLDSAVIHHVG
                     DGGVTNSPQAIENAHMVGLGHYDVGYHFIAGADGTIYEGRYIGARGYHVQKYNTGKIG
                     VVLIGDFSGDISPTQIQVDATLGLLLYLDAKYGIDFVNGHSDYPDQGNTICPGQNMAK
                     PINMFRSFIE"
BASE COUNT         1787 a         2089 c         1858 g         1949 t
ORIGIN      
        1 atgcccgctc gggtcggtgt accgcaccgg gttgttcatc acatgttagc atataacaac
       61 tggccagcca gtccgtctcg tatcttgcgg gacggtacgt ttggacgcat aattcctttg
      121 gctgagtgaa cgggaacgtt gcgcccgggt gcgtgacgta tgggcaagcc gggtatgctt
      181 atggaaagtc tctcaaagac aagtccgctc acatgtttgg ctgcccgttc gaatccgctc
      241 acctgagaag ggctttggtt gccgccacag ccctagctcg aatccgtgtg tgatgtcacg
      301 cccaaaaaca aacgcatccg gcctgactgg ccaggtttgc cagcatattc gaatggaggt
      361 tatgagaata tgccgcacat tgattctact cccaaacgtt acatcggttt cgatattcac
      421 aaacactact tcgtcgccat cggcgtcgat ccacagctta atccggtatt tggaccgcac
      481 caggcttcca ttacgcaact ggctgaatgg gccaggaaga acctgacccc tcaagacgca
      541 gtcgtggtcg agatgaccac aaacacctat ctgtttttcg acatcatcaa gccactggtc
      601 cattcggtca cagttgtgca tccaccccat gtttccctca tcgtacgcgc tcaggtcaaa
      661 acggacaaga aagctgctct caccctggcg caattacatg cctcgggttt gctgccgggt
      721 atctggatcc cgccggttga agtgcgggaa ctgcgcgccc tggtggctca ccgcaataaa
      781 atggtgcatt tctccagcat cgccaagtgc cgccttcatg cgctcctgca gcgtaaaggg
      841 attgaagccc cgcaaggcgt ggaacctttc tcacccgaaa tgcgcctctg gtgggatgaa
      901 cttgacctga ccgccttgga aaagcattgc atgcactctg acctgtccat gctggatttc
      961 gcccggtctc aggtggaatc cgacgagcag tgtctcaaac acctggcggg caaggatgaa
     1021 cgccttccgt tactcattca aatctgtggg atcgggttta ttaccgccat caccatcctg
     1081 gctgccatcg gcgagattca gcgttttcca accccagggc aactggtggg ttatgccggt
     1141 ttgggtgcca gggtgcacga cagcggcatg agccgcacca ctggccgcat taccaaaacc
     1201 ggcaggcgcg atctgcgccg cgccatggtg gatgctgcca atcatgccat tccattccat
     1261 ccccattgga gagccgtgtt tgaacgtcaa tcaatgcaca ttggcaggca gaaaacgatt
     1321 gtggccattg cccgcaagct gctggtagcc gtctggcatg tattaagcca ccaggaggcc
     1381 gatcgttttg ccgatcacca gcaggtggcc acggcgcttt tcgcttatgc ccatcgcatt
     1441 cgcgttcaga acctgccgga tgaacaatct gctctgcagt tcacgcgcaa ccagctcgac
     1501 cggctgaaga tcggtcagga ggtcaggcaa atcccgtggg gctcgcggaa gttcaagctg
     1561 ccggagagta agctgaccgt gagctagtgt gggcttggat aactgaataa gcgtgtagaa
     1621 cccgtttcgt tcaagcgggc tgtattctta tttggttttt aaggttcgag gacctgttcc
     1681 gtactatact gaaaacacac cccaagagaa aggcgagctg aggcggtctc atgacccttc
     1741 gctcgtcttt caattaccaa ataggacatt cttaagagga atgttatacc gcactatcac
     1801 aatgatagtg gcaatgatta ataagcctgt ttcaattatc cattttcgag taggccactg
     1861 ttgattagct tttacaaaag ttgttgaggt ggtagacgtt aggaacaagc ccctccaacg
     1921 atcataaact tttggaatgc caagtaatat atgtcgaaga cataaaatcg caatcgacca
     1981 gccaattccc caatatacgg caattaatgc tacacaaaga ttaaaaccaa ctacccaaag
     2041 caatgaagcc aaccccagtc ttccattcat cagttgacct ccggataatg atttggcata
     2101 gagggtatgt tttgattggg tatggctgat atatttagca cttcttctat ttttccatgt
     2161 tccgcatctt gccacatttt aacaccaaaa atattgacag gtagtaccac gagagtttct
     2221 aatgcttcgc cagctttcat taagacaggt tgacctgtta acatcaagcc gaatgctgga
     2281 ataccaactg ttaaatcggt ggctgcgata agaaatgccg ccgctacttt tttggcgtcg
     2341 tcagatccat agtgtattag agagtaggtc gacccatatt tcgctgcctt cacctggtcc
     2401 atataactac tttctccagc tatgattaat cggcctgctt gatccaacgt aaacttacca
     2461 gtaaatgacg actcaacatc tggatgatta ttgggtagtt gatctgtatc ccagttaatg
     2521 ttgatttcat ctcctaattc agctttctgt aataccgtta gccaccccca acgcccctct
     2581 agagtacctc cttcttgaaa tacccttaag acatcatccc aagagtcgac accgaagtag
     2641 tttttgattt catctttatt aaaatgacct gttggatcgg tatatcttga cggattgtta
     2701 tatccgtacc cataccggtc ccacgccatt gggttccccg gctggggaat gaggctgtcc
     2761 gcctgcacaa acctgcccag ggcggatcgc ttatcttaac cacctcctca tgggaaatcc
     2821 tcccgagagt agaataaggg taccaagcca cattctaaat ctcaggaggt tctttcatgc
     2881 tatccgaaat cgaccagttc gtcaactggg tgcgcaggcg aagccccaat gcccgcacct
     2941 ggaaggacta cagctacgac ctgcatcagt tcgccagttt tatcggcgat ctcccggcaa
     3001 catccgttac tttccgcgat gtcgactgct tcgttgctca ccaggtatca cgtggcttcc
     3061 aacccaccac cgtcaaccga cggttagcct gcctggtttc tttcttcacc ttcctctcgg
     3121 atgaggaccc atctttagtg tgtccagtgc tacccaaacg ccacttcctc cgtgagcggg
     3181 agcgcctgcc ccgtcccgtc cagcccgagc aacttaaggc cttcttcata gccattgacc
     3241 atgccattca atctggctgc tccgaagctc tccgcgatcg ggccatgttt ctgctcatgc
     3301 tgcgctgtgg gttgcggatc ggcgaggtcg ccaacatgct cctcaatggc ctctacctgg
     3361 atgaacctcg gccgcgcctg gtggtcagcg gcaagggttc ccgcgagcgg gtcgtctaca
     3421 tctcgcctca ggctgaacgc gccctgcgtc gctatctggc tctccgtccg gacagcctca
     3481 gcgaaaacgt cttcctggct taccaccgtg atgggctgtc caccactgcc atacacaagc
     3541 ggctcatgca ttaccgccag gaagcgcaag tttcactcac cgcccatcgc ctcaggcatt
     3601 ctttcgccaa cgacctgctg accgctgatg tgcccatcac aaccattcag aagctcatgg
     3661 ggcatcgctg gttggagacc actcagatct acgtccaggc taacgaccgc caggtagccg
     3721 ccgattacta cgctgcttgt gccaggctgg aggattggtc atgagccctg ccaaagccgc
     3781 ggttacccca tgtgagatct acaatcacgc actcaaatat tcacgccacg accatgtgcc
     3841 gccaggagtg ccgctaccca actccaccaa cacctggttg ccggagaata ttgccatatt
     3901 ggaacgttat gttacctggt tggccggggg aggaaccagt gcctacgtca ttcgcgtgat
     3961 ccacatccct atggcagggc atattctcag cctaaaccat aagccatcca ccgcgcttga
     4021 cctggacaac gatctgcaag caggcatgga ctacattctg gctaaagggc tcggcttttg
     4081 ctggacaaaa aactgccgcc tgtcgatgct caaattccgc cgatttctgt tgcacgagcg
     4141 cgggcagacc gagtgccaca tcacgccctt tgatcctgtt cttcataccg agggtttacc
     4201 cgcctggctg gtggaacagc ttacccgcta ccagcatgtt aagcagcgca attggcgcga
     4261 atcccggata gaggatggga ttcgtcgctt ttggtgtgag catttacgcc tctggcgctt
     4321 cctggtcaga cggtttggca tccaggcgtt atctgacctg aaacgcagcc actttctaga
     4381 ctacactgac tggcggctgc aagccgggat gtcggtacgg ggcgtgaacg gcgacttgcg
     4441 cacctttcac ggcttcatgg aatttctgca gggacaggat tggccggttc cgcatatctt
     4501 gctgcgactg ccttgtctca aagagcccga ttcactgcct aaattcctga ccgatactca
     4561 agtgtgctcg ttacgggatg atttggaggc gcgggtagcg ggtgctgaca acgctgccca
     4621 ctgccgggat gcgcttctgg atcgagccac attctacctg ttgtggcaag gcggtttacg
     4681 gcggggcgag gtggaagact tgcgcctgga ggaccttgac ctggagggcc gtaagcttac
     4741 cgtgcggcgc ggcaaggggc tggttgatcg caccgtgttt ctctctcaat cggtggtgcg
     4801 cgttctgctt gcttacctgc ccatgcgcgg gccaggaccg accgaccacg tatttctcta
     4861 tcgcaaccag gccatctcca aggatctcat ccacggtagg ctcaaggcct gtggagtgcg
     4921 ggtgggggta tcggtgtatg ctcatcgctt gcgccacacc tgcgccacgc agttgctcaa
     4981 tgcgggctgc ccggtaacca gcatccaaaa attcctcggt cacaagcgtc ttaataccac
     5041 gatggtgtac gccagagcat acgaccagac ggtggaggcc gactactttg ccgccatgag
     5101 ccgggtggaa cagcgtttgt tggttgcacc agaaacggaa ccgacaatag cagtggtcac
     5161 ggacgagaag aaaatgcaaa ttctggccat tgccgggcag ttggccgctc cggaagccga
     5221 gccagagatg cgtagaaacc tgtttgagca gatgcgctac ctgctgtttg gcgggcagac
     5281 gttccctgag gcggttttgc cagttgaggc tggtagccag gtttggatcc cgccgccggt
     5341 ggatgtggct gtgggttagg ggatattatt caatttgtaa ggtgcgaacc cgctcgcttt
     5401 caggcgtcgc cgggagttgc aattttcaca aaagaccttt gcttaccatc aatgcggagg
     5461 cggggttaag gtcggtgacc ccggcgtgat aaatagctcg ggattacgaa acccacattc
     5521 caaataattg tcttttttaa gataaacgca atactttctt tctgatgggt cgaatcccac
     5581 ttcgtatgtt tggtattcat tgcgttcata ccaaatttcg tgcccatcaa atgtataagg
     5641 taattgagca atatatcctg ggattaaatc ggtgatcaaa tcaggataaa ctccgtgatc
     5701 ggcataatat ttttcgaccg cctcgacaac aaccatgagt tcacctttcc caattgtgat
     5761 cggatgtggg gtcagggttt gtgtaggtat ggcagtccta tcctggagac tgtttatctt
     5821 tggctgttcg gtctcagcgt taatattgtc acaggctgaa aggccacaaa caagaagtat
     5881 tagtagggcc agcttaaaaa gatgtcgaat gctcattcga tgaaactccg gaacatatta
     5941 atcggctttg ccatattttg ccctggacag attgtgtttc cctgatcagg ataatcagaa
     6001 tgcccgttga caaaatcaat accatatttg gcatctaaat acaacaataa gcctaaggtc
     6061 gcatccactt gaatttgggt cgggctgata tcaccgctaa aatcgccaat taatacgacg
     6121 ccaatttttc cagtattata tttttgaaca tgatatcctc ttgctccaat atatcttccc
     6181 tcatagattg tcccgtcggc acccgcgata aaatgataac ctacatcata atgtccaagc
     6241 ccaaccatat gcgcattttc gatggcttgt gggctattag taaccccacc atcacccaca
     6301 tggtgaatga cggcagaatc taaagtatca gccaaattgc cgggatattc gtagtaaccg
     6361 ccgaattcat tgtttggatc ataggtttcc ctatcttcat ctgaaaaatt acccggattg
     6421 tcacccggtg cgtattgccc ccaatctttt cgggctacga ttggcaaagt tgtttttcca
     6481 cctgccaatg cattcatacc cgttgagcgc atcattgcac cagcctcagc gaacgtttct
     6541 acgcaacccg tatcataata atctccacat gcccaatgtc ccgtcggatc cgtatatttc
     6601 agggggttat cccgtacata ggagtagcga ttccagtctt gtggatttcc tggatccggt
     6661 ataatactat caggctgtac ccagcggtta agataagggt cataccagcg ggtgttgtag
     6721 tacatcagcc caatctccgc catgttcgag tactgcccgg tgtaggtgta ttgggtgggg
     6781 gtggtgccgc tggtataccg cacttctcca aacggtttgt acaacatctc tgccaccaaa
     6841 ttgcctgcac tgttcgtcgt tacactggtg ctgcccaaat ggtcgcccag caggtagttg
     6901 ggcggcgcca gcatgccgtt ctgcaccgcc acccgctggg aaccagcgta atagtatttc
     6961 gtaatcacct gctcttccaa gctcacactc gcatcatccc acgccagcca gccggagaac
     7021 tgcgtgccgg ataattccag cttcagcgtc acagcgtcca gcgggctggt tatggtcttg
     7081 gtcacttgtg tccagtcagt agacagttca gcgctgctca taaccgcctt acactcagag
     7141 agggtattgt caatccggta gaagcaggcg ttgatctgcc agtctccggt tggaaagact
     7201 gttttggcga cttttgtggt ttctgttgga atttgcgcat tgctttacag tctcagcatg
     7261 tcctcctgag cacttctact caggggtata gctctttttt gatttcgcca acaaactcga
     7321 ttgcttaaat tactcctgtt tcttcctttc caggtaaaag gcgaacgccc atactggccc
     7381 cccaaaaacg agaatatcta ataatattcc ccagccacct ttagggtacg ttattattct
     7441 caaagtgaat gttatgccta aaaagatcca aaataagcct aacaactgaa aaaaaacaac
     7501 gccaggaaat ttacgacaac tcgtatttgc tacatactta tctttgctct ggcatatttt
     7561 tacttggata aaagtagaaa ttacagctag aagcaaacca acaataagaa aaaaaacgcc
     7621 catgatacta ccttttgttc gtattacttt gcaattggcg acattacttt atgtattgta
     7681 taa
//
LOCUS       DAOPVS010000092         7619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9637_length_7619_cov_39.996034, whole genome shotgun sequence.
ACCESSION   DAOPVS010000092 DAOPVS010000000
VERSION     DAOPVS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9637_length_7619_cov_39.996034"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKL11_07895"
     CDS             <1..761
                     /locus_tag="PKL11_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13564.1"
                     /translation="QQQEQQLPFLKSIKESFSNFAFVIFLVLSFSIMYIQTSLMQGVA
                     YYFDEVMGTALPAYIGLGVGIVTGIILWVNRRDRWGVKRCLAIWLGLFALGCLLLLTL
                     GHTIPGAVVGFFLVGIGFAGGMYLIPIMNGDVIDYDEERTGLRREGMYAGINSLVTKP
                     AISLAQWAFLSIIAANGYDQSLTKGLQSSQAETGILIAWTLIPAILLVISIIAMYWYP
                     LAGEKWESIKKKLTVVHQEKEKQFLESLGYRFVK"
     gene            921..1940
                     /locus_tag="PKL11_07900"
     CDS             921..1940
                     /locus_tag="PKL11_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ13565.1"
                     /translation="MRKEVVLITGANGEIGHDLITHLGKNGEAHVIALDVAPLDDALR
                     PYCERFIQGDILDGLLLGRLVAEYEVRTIFHLASILSTKAEYNPETAHRVNVEGTLNL
                     LRLAVEQAAWQGRPVKFLYPSSIAAYGLPNIPTKNAAGKVKEDAYLQPKTMYGINKLY
                     CEHLGRYYTYHYRQLAADTPKNSVDFRCIRFPGLISAQTVPTGGTSDYGPEMLHHAAQ
                     GLAYACFVRPDSQLPFMVMADAVKSLLELEAAPVEKLSQRVYNVTSFSPTAQEIYEIV
                     LNAFPKAEITFSPHASRQGIVDSWPGDVDDSAARQDWGWKPDYDQKRSFDEYLIPAIR
                     ERYHV"
     gene            1982..2539
                     /locus_tag="PKL11_07905"
     CDS             1982..2539
                     /locus_tag="PKL11_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13566.1"
                     /translation="MSEQTPERPYPVGSDEKVNRVMVYTSNAIYWGDVVAKEAVRVST
                     WLRTNSAPEFLRIYQARCIVPTAGATPRPLLYTEIYIPATEIRAYHLMAPDKDPLDYD
                     PTEPNRRMDPVSVVVGTFRIDGHLRVSAISNLGKYLEITREAYTPLYDVEITNLIMPA
                     LGVMKTYYIQVRQSTGLFGILPQTA"
     gene            2563..3546
                     /locus_tag="PKL11_07910"
     CDS             2563..3546
                     /locus_tag="PKL11_07910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNZ13567.1"
                     /translation="MPKPRIFMTRAIAPAAIERLSAQTDLEVWPHEAPPPYEILIDRV
                     QNATGIVSLLTDRIDAGLIAAAGPDFRIIAQMAVGFDNIDVAAATARGIPVGHTPGVL
                     TETTADFAWTLLMAAARRVVEAHNEVQQSIWRPWGPDVLTGQDIFGATLGLVGFGRIG
                     QAMAKRAQGFDMPILYHDLQRFPEAEKSLGAQFVSLEELLERADFVSLHTFMSPQNKF
                     MMGKSQFGQMKPNAIFINTARGGLVDPDALLWAVRERKIAGAGLDVFETEPIPEGHPL
                     LNEPSVIITPHIASASKNTRARMALMAAENIIAAIEGKRIPYCANPEVYDA"
     gene            complement(3534..4895)
                     /locus_tag="PKL11_07915"
     CDS             complement(3534..4895)
                     /locus_tag="PKL11_07915"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNZ13568.1"
                     /translation="MLPFIEKCLFTQLAELGRFASPVSQVITAALSAVDPRIATRSAL
                     EIAGLDSSQDPAGRLMLLAFGKAAFPMAMGALDVLAGCISGGVIVPKHIPSDLDALPA
                     AFSLIPGAHPVPDERSQTAGLVVYDFLSNLTDQDRVICLISGGGSALLTYPVDGVSLT
                     DLQQLTHQLLACGASITEINALRKHLDRVKGGGLARSAAPAKLDTFILSDVMGNPIDA
                     IASGPTAPDPTTYAEAWSILEKYHLESIAPAPVISVLRKGLEGLRPETLKPGAPIFRR
                     VTHTIVADNAAAAQAAAARSQSLGWQSNWDPTPLCGEARHAGVQLATTLSHLAAQHNS
                     NQSLFFQVLGGETTVTLQGNGLGGRNLETALAAVPILAGVKNTALITLATDGEDGPTD
                     AAGALVTGETMQKARQMNLNPADFLARNDSYTFFQQVGGLIKTGSTCTNVNDLVFLLS
                     YAS"
     gene            4996..5565
                     /locus_tag="PKL11_07920"
     CDS             4996..5565
                     /locus_tag="PKL11_07920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ13569.1"
                     /translation="MKFWPVPHSFSRTLPAEGDAGCFWQDRGDRRHAGVDIYAPAESD
                     VVAIESGVVRSVELFTSPELRTYWNNTYAIVVQNSAGQVIRYAEMKSACVQPGDLVQA
                     GQKIGQVGQVLEPDRIDHTAPGYIQALKANGVASMLHLEVHTGLPLDDAQYLGGNYFN
                     RPQPQGLINPEVVLAELVENHPQKANGGF"
     gene            5636..>7619
                     /locus_tag="PKL11_07925"
     CDS             5636..>7619
                     /locus_tag="PKL11_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNZ13570.1"
                     /translation="MNVTNLDSLYRPKSIAVVGASNTPGKIGFTVVDNLLKSGYEGKI
                     FPINPKDPEVQGLKAYASVLAVPEAVDAAVIVVPASMAEQVTEDCGKKGVKALIVIAS
                     GFSEVGNRELEDRVVAIAKRYNMRILGPNIVGTLSNSDKMNASFAPFLPFAGKAALIS
                     QSGALLIAIDASTYIRGIGFDKLISIGNMSDVDFADTIAWLDNDDATSCVTLYIEGFK
                     DGRKFMEVARKAHKPIIALKSGVSAHGAAAAASHTGSLAGAAKVYGAAFQQCGVVQAA
                     DLDNLFDRTLALSLQPPMKGDNLLIITNGGGVGVLATDAAEHCGLPLKFAPAEVQTEL
                     KKHMPEFGSAKNPVDLTGMAGRDWYTDSLRYAIAHPWVDGIAVLYCETSLTDPVDIAR
                     GIKQALDEAGVKDKPVTVSFVGGEKSLEATRWLINNGIPCYGAPDLAVNALSALREYD
                     RMRAALAEPETACQSVDRARALEVINLARTDGRDSLTEIEAKQVFSAYGLPVTSTFLA
                     KTEDEAVALAKKIGFPIVMKIVSPDILHKSDAGGVKVNLKDEAGVREAYRTIMTNAKA
                     YKADANIHGIAIQEMAPWGTEVILGSVNDATFGPTMMFGLGGIFVEVLKDVTFRVAPV
                     SEGEATRMLGEIRGAPILAGARGEKPRDRKALADIIC"
BASE COUNT         1767 a         1961 c         2169 g         1722 t
ORIGIN      
        1 tccagcagca agagcagcag ctcccctttt tgaaatccat caaggagagt ttttcaaact
       61 ttgcatttgt cattttcctg gtgttgagct tttcaatcat gtacatccag acttcgttga
      121 tgcaaggcgt agcgtattac ttcgacgaag tgatgggcac agccttaccg gcatacattg
      181 gcctgggtgt tgggattgtg accgggatta ttctctgggt aaatcgtcgg gaccggtggg
      241 gggtaaagcg ctgcctggcg atctggctgg ggctctttgc attgggctgc ctgctgctgc
      301 taaccctggg acacactatt ccgggagcag tggtgggttt tttcctggtc gggattggtt
      361 ttgccggcgg catgtacttg atcccaatta tgaatggtga tgtgattgac tacgatgaag
      421 agcgaaccgg gctgcggcgc gaaggaatgt atgcgggcat caatagtctg gttaccaagc
      481 cagcgatcag cctggcgcaa tgggcgttct tgagcattat tgcggccaat gggtatgatc
      541 aatccttgac aaaagggctc caatcatcac aggccgaaac ggggatttta atcgcctgga
      601 cgctgatccc ggctatcttg ctggttatct cgataattgc aatgtactgg tacccgctgg
      661 caggggagaa atgggaaagc attaagaaaa aattgactgt tgtgcatcaa gaaaaagaaa
      721 agcaattcct tgaaagcctg ggatatcgct ttgtgaaata agcaaacagt tacgggatgg
      781 cgctgccacc ccgtaactgt ttaaaagtgt caaaaatcat cccctgtgac tgccccgcgt
      841 tttttgaata ttgatacaat aaagaaaatc ccgcctccag tcgggagatt taccatcctt
      901 ccagtattga gagaggtctc atgcgcaaag aagttgttct aatcaccggc gccaatggcg
      961 aaattggaca tgatttgatc acccatttag gcaaaaatgg cgaagcacac gtcattgcac
     1021 ttgatgtggc tccgctggac gacgcgttac ggccctattg cgaaagattt atccagggcg
     1081 acattctgga tggcctgctt ctggggcgtc tggttgccga gtatgaggtc cggacaatct
     1141 tccatctggc ctcaatcttg tccaccaagg ctgaatacaa tccagaaacg gcccatcggg
     1201 tgaatgtgga aggaacgctt aatctgttga gactggcagt tgagcaagct gcctggcagg
     1261 ggcggccggt caaattcctg tatccaagct caattgcggc ctatgggctg cccaatatcc
     1321 ccaccaaaaa cgcggcgggc aaggttaagg aggatgcgta cctgcagccc aagaccatgt
     1381 acggcatcaa taaactttac tgcgaacatt tgggtcgcta ttacacttat cactaccgcc
     1441 aactggcagc ggatacgccc aagaattcgg tagattttcg ctgcatccgc ttcccggggt
     1501 tgattagcgc acagacggta cccactggcg gaacaagcga ttacggaccc gaaatgctgc
     1561 atcacgcggc ccaggggttg gcttatgcct gcttcgtgcg gcccgattct caactgccgt
     1621 tcatggtcat ggcggatgcg gtcaaatcct tgctggagct ggaagctgcg cccgtcgaga
     1681 aactctcgca acgggtttac aatgtcacca gcttctcccc cactgcgcag gagatttatg
     1741 aaattgtttt gaatgccttc cccaaagccg agattacgtt cagcccacat gccagccgcc
     1801 agggaattgt ggactcctgg ccgggggacg tggacgattc ggcagcccga caagactggg
     1861 gctggaagcc tgattatgat caaaagcgct cattcgatga gtatttgatc ccggccattc
     1921 gcgagcgcta tcacgtttag ctgtccgttg gcgcgtccag cggctatacg aaaggtttgc
     1981 tatgtccgaa caaacacccg aacgtccgta tcctgttggt tcagatgaga aggtaaaccg
     2041 cgtgatggtt tacaccagca acgccatcta ctggggagat gttgttgcca aggaagctgt
     2101 ccgggtaagc acctggctgc gtaccaactc ggcgcccgag tttttgcgca tttaccaggc
     2161 ccggtgtatt gtgccgacag ccggtgcaac tccgcgccct ttgctctaca ctgaaatcta
     2221 tattccagcg actgagattc gcgcctatca cctgatggcg ccggacaagg acccgttaga
     2281 ttacgacccc acggagccca accgccggat ggatcctgta tctgtcgtgg tgggcacgtt
     2341 ccgtattgat ggacatttgc gggtttcggc aatttcaaac ctgggcaaat atctggaaat
     2401 tacccgagaa gcctacacgc cgctgtatga tgttgaaatt accaacctga ttatgccggc
     2461 gctgggtgtg atgaaaactt actatattca agttcggcag agcaccgggt tgttcgggat
     2521 tttgccacaa accgcataat ctcaaacaca ggagaacagg gcatgcctaa accgcggata
     2581 ttcatgacgc gcgccatagc accagcagca attgaacgct tgagcgccca gacagatctg
     2641 gaagtttggc ctcacgaagc cccaccgccc tacgagatat taatcgaccg ggttcaaaac
     2701 gccacgggga ttgtctcgct gttaacggac cggattgacg caggtttaat tgcagcagcc
     2761 ggacccgatt tcaggatcat tgcgcaaatg gccgtcgggt ttgataacat agacgttgca
     2821 gcggcgactg cgagaggtat tccggtggga cacactcccg gagtactgac cgagacaacg
     2881 gcggatttcg cctggacgct gttaatggcg gctgcccggc gagtggttga ggcgcataat
     2941 gaagtccagc agagcatctg gcgcccctgg ggtcccgatg tcttaactgg gcaggatatc
     3001 tttggtgcaa cactgggttt ggtgggcttt ggtcgaattg gtcaggccat ggccaaaaga
     3061 gcccagggtt ttgatatgcc aatcctgtat catgatttgc aacggtttcc ggaagcggaa
     3121 aaaagcctgg gagcacagtt tgtatcactg gaggaactat tggagcgagc cgattttgtc
     3181 tcgctgcata ccttcatgtc gccgcaaaac aaattcatga tgggaaaaag ccagtttgga
     3241 cagatgaagc cgaatgcgat cttcatcaat accgcccggg gtggcctggt tgacccggat
     3301 gcgcttttat gggctgttcg ggagcgtaaa atagctggcg ccgggctgga cgtttttgag
     3361 accgagccaa ttccagaagg acacccgctg ttgaatgagc cgagcgtaat catcactccg
     3421 catatcgcca gtgccagtaa aaatactcga gccagaatgg cgctgatggc tgctgaaaat
     3481 atcatcgcag cgattgaggg aaagcggatc ccatactgcg ccaacccgga agtttacgac
     3541 gcataagaca gcaggaatac caggtcatta acgttcgtgc aagtggaacc agttttgatc
     3601 aacccgccga cctgttgaaa aaaagtataa gagtcattgc gcgctaaaaa gtccgccgga
     3661 tttaaattca tctggcgggc tttttgcatg gtttcaccgg taaccagggc gcctgccgcg
     3721 tcggtgggac catcttcgcc atcggttgcc agagttatca gagccgtatt tttaacgccc
     3781 gccaatatcg gaacggcagc cagggcggtt tccagattgc gccctcccag cccgttaccc
     3841 tggagtgtga cggtggtctc tccgcccaac acctgaaaaa acagcgattg attggagttg
     3901 tgctgcgcgg ctagatgcga taaggttgtg gctagttgaa cgcccgcatg tcgggcttcg
     3961 ccgcaaagtg gggtcgggtc ccaattggat tgccagccca ggctctgtga ccgggctgca
     4021 gccgcctgtg ccgcagcggc attatcggca acgatggtgt gggtgacacg ccgaaaaatt
     4081 ggggctcccg gtttgagtgt ttcagggcgt aggccttcaa ggccctttcg gagaaccgaa
     4141 atcacggggg cgggggcaat tgattccagg tgatattttt ctaaaatcga ccaggcttcg
     4201 gcgtaggttg tagggtcagg tgcggtgggg cccgaagcaa tggcgtcgat ggggttgccc
     4261 ataacatctg aaagaatgaa ggtatccagt tttgccggag cggcggagcg agccagacct
     4321 ccacctttga cacggtcgag gtgtttgcgt aaggcgttga tttcggtaat ggatgcgccg
     4381 caagcgagta actgatgggt taactgctgc aggtcggtaa gggaaacgcc atcaaccgga
     4441 taggtgagca aagcggaacc gccgccagaa atgagacaaa tcacgcggtc ctggtcagtg
     4501 agattggaca ggaaatcata aactaccaga ccggccgtct gactgcgctc atctggcacg
     4561 gggtgggcgc ctgggatcaa ggaaaaagcg gcgggcaaag catccaggtc tgatgggata
     4621 tgcttgggca caatcacgcc gccagaaatg cagccggcaa gtacatccaa agcgcccatg
     4681 gccattggaa aagcggcttt accaaacgcc aatagcatca atcgtccggc agggtcttga
     4741 gaggaatcta ggccagcgat ctcaagagca ctgcgggttg caatccgggg atcaaccgcg
     4801 gataaggcgg ctgtaataac ctgcgatacg ggggaagcaa accttcccag ttcggcgagt
     4861 tgagtgaata agcacttttc gataaaaggt aacattttta ccctcaaatg taatgataaa
     4921 tatacacccg aaatcaggac ttttgttttt aaaaatatgc gtacaataag accggtctgc
     4981 gcgaggagaa ttttagtgaa attctggcct gttccacata gcttttcccg cactttaccg
     5041 gctgaaggag atgccggttg tttctggcaa gaccggggag atcggcgcca cgcaggggtt
     5101 gacatttatg cgcccgccga aagcgatgtg gttgccattg aatccggcgt tgtgcggagc
     5161 gtggaacttt ttacatcgcc cgaactgcgc acatactgga ataatactta tgctattgta
     5221 gtccagaatt ctgccggaca ggttattcgg tatgcagaaa tgaaatctgc ctgtgtacaa
     5281 cccggtgatt tggtgcaagc cgggcagaaa attgggcagg ttgggcaggt gctggagcca
     5341 gatcgcattg accatacggc gccgggatac atccaggcgc ttaaggctaa tggggttgcg
     5401 tccatgctcc atctggaggt gcacaccggg ctgccgctgg atgacgcgca gtatctgggc
     5461 ggcaattatt tcaatcgacc ccaaccccag ggcttaatca acccggaggt tgttttggcc
     5521 gaactcgttg aaaaccaccc gcaaaaagcc aatggcgggt tttgaggtat actatacctt
     5581 tggtttaatc taataaaaga aaaagtttct ttaatacctt tcaggaggat caagcgtgaa
     5641 cgtaacaaat ctggatagtc tctatcgacc caagtcaatt gccgtggtgg gcgcctcgaa
     5701 caccccgggg aagattggct tcaccgtggt agataacctg ctcaagagcg ggtacgaggg
     5761 caaaatcttc ccgattaacc ccaaagatcc cgaagttcag ggactgaagg cctatgcttc
     5821 tgttctggca gtgcccgaag cggttgatgc tgcggtgatt gttgtgcccg cgagcatggc
     5881 agaacaggtt acggaagact gtggtaaaaa aggcgtgaag gccctgattg tgatcgcttc
     5941 gggcttttcc gaagtgggca accgtgagtt ggaagaccgg gttgtggcga ttgccaaacg
     6001 ctataatatg cgcatcctgg gtccgaacat tgtgggcacc ctgtctaact cagacaaaat
     6061 gaatgcctcg ttcgccccct tcctgccctt tgccggcaag gccgccctga tttcgcagag
     6121 cggtgcgctt ttgatcgcca ttgatgcctc cacttatatc cgcgggattg gttttgataa
     6181 gctcatctcc atcggcaaca tgtcggatgt ggattttgct gacaccatcg cctggttaga
     6241 caacgacgac gccacgagct gcgtcacgct gtacattgaa ggcttcaaag atggccgcaa
     6301 attcatggaa gttgcccgca aggcgcacaa accgatcatc gccttgaaat ctggcgtttc
     6361 ggcccacggt gcagcagcgg ctgcttctca cacgggttcg ttggctggtg cggccaaggt
     6421 ctatggcgcg gcgttccagc agtgcggcgt tgtgcaggct gctgacctgg ataacctctt
     6481 tgaccgcacc ctggcgctct cgctgcagcc tccgatgaag ggtgacaacc tgctcatcat
     6541 caccaatggc ggcggcgtgg gtgtgctggc gaccgatgcg gctgagcact gcggcttacc
     6601 gctgaaattt gccccggctg aagtgcagac tgaattaaag aaacatatgc cagaatttgg
     6661 ctcggccaag aacccggtgg atttgaccgg catggctggc cgcgactggt acaccgactc
     6721 gctgcgctat gccattgccc acccgtgggt agatggtatt gcggttctgt actgcgaaac
     6781 ctcgctgact gacccggttg atattgctcg cggcatcaaa caggccctgg atgaagctgg
     6841 cgtcaaagac aagcccgtca ccgtttcgtt tgttggcggc gagaaatcgc tggaagccac
     6901 ccgctggctg atcaacaacg gcatcccctg ctacggcgcc cccgacctgg ctgtgaatgc
     6961 cctgtctgcg ctgcgcgagt acgatcgcat gcgcgccgcc ctggccgaac ccgaaaccgc
     7021 gtgccagtct gtagatcgcg cccgggcact ggaagttatc aacctggccc gcaccgatgg
     7081 ccgcgattcg ctgaccgaga tcgaggccaa gcaggtcttc tcggcttatg gcctgccggt
     7141 aaccagcact ttcctggcga aaaccgaaga tgaagctgtt gcgctcgcca agaagattgg
     7201 cttcccgatt gtcatgaaga tcgtatcgcc ggatattctg cacaagtctg atgctggcgg
     7261 cgtgaaagtc aacttgaaag acgaagctgg tgtgcgcgaa gcttaccgca ccatcatgac
     7321 caatgccaaa gcttacaagg ccgatgccaa tattcatggc attgcgattc aggaaatggc
     7381 tccctggggc accgaggtca ttctgggctc tgtcaacgac gctacctttg gcccgaccat
     7441 gatgttcggt ctgggcggca tcttcgtgga agttctgaag gacgtgacct tccgtgtagc
     7501 gccggtttct gaaggcgaag ccacccgcat gctgggtgaa attcgcggcg ctcccatcct
     7561 ggcaggcgcc cgcggcgaaa aaccccgcga tcgcaaagcc ctggcggaca tcatttgcc
//
LOCUS       DAOPVS010000093         7552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9758_length_7552_cov_45.424837, whole genome shotgun sequence.
ACCESSION   DAOPVS010000093 DAOPVS010000000
VERSION     DAOPVS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9758_length_7552_cov_45.424837"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1400)
                     /locus_tag="PKL11_07930"
     CDS             complement(75..1400)
                     /locus_tag="PKL11_07930"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNZ13571.1"
                     /translation="MQKSNLVPLLLAIVLIALMAPAPAPVLAQGTTWDIYVNDPRDIR
                     DYGINSVCDADYNTPGPQCTLRAALDEANYWNLSDDAVYIHLPPGHYTLTLDGRNEND
                     NESGDLDIYQTDAQIIIEGESAASTVIDANGLDRVFHIRNTAWVDLRNLTITGGHTEI
                     PTDPFAYGAGIYNDGSDLTLDSVIIENNSVVCLPGTDNCSQASGGGLFNISANIIVNQ
                     TTIRSNWASRGAGIFNTDSAYTLTLLNSTISGNHATVAGGGIINYGTMQMINDTISGN
                     TSDLTSGGISNDAAMYLANVTVARNIAANNAANLYNSSGAAVYLKNSIVANALGGDYV
                     TNCNGNGTWNTAGYNISDDATCHLTAGSDQTNTDPLLTPLAYLGGLTPTHGLLPGSPA
                     SDNNPSGCTNFEGYTVTADQRGYNRDFVGCDAGAFEGIARVINLPAILR"
     gene            1509..2009
                     /locus_tag="PKL11_07935"
     CDS             1509..2009
                     /locus_tag="PKL11_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNZ13572.1"
                     /translation="MSLIRCRFVLCFLTRADQVLMLLRNRPPNQGLWNGVGGHIEPGE
                     APLAACLREVREETGYTLPTVRFGGVLTWEGFEIEPGGLVLFTAEAPAGEPVDCSEGL
                     LAWQPQDWACTAPQVVGNLHVVLPCLLRGEPPQTYHFRYSDGQMLGWAQGPAPADMMT
                     VEIKGA"
     gene            2015..2356
                     /locus_tag="PKL11_07940"
     CDS             2015..2356
                     /locus_tag="PKL11_07940"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ13573.1"
                     /translation="MEDFTWAREFPGAITVCDTQGIIVYMNDRSAKTFEKDGGAALVG
                     QSLMDCHSAHSQEMIRQMLETHERNVYTIEKNGVRKLIYQSPWYQNGEFAGLVEISLE
                     IPEQMPHFVRG"
     gene            complement(2353..3642)
                     /locus_tag="PKL11_07945"
     CDS             complement(2353..3642)
                     /locus_tag="PKL11_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ13574.1"
                     /translation="MRIAIIGAGISGLSAAHDLLKAGHSVTIFEAADHVGGLAAGFKE
                     PHWDWSVEQFYHHWFQTDAEMLGLIRELGWQDDVLFPRPKTVVYYKDDFYPLDSPLAA
                     LTFPGFTLIDMARFGFVTAYLRYLAAWQPLEKVTAHEWMRRYYGERLYATQFEPMLIG
                     KFGPHYKEVNMAWFWARFKTRSTRLGTFIGGFQAFLDRFAENLRSRGAEIHLNTPVQQ
                     IEPLAEGGLCLRLPGAEPLTFDRILSTTSPALMARLTPALPREYLQGLLNLKSMGAVV
                     MVLAIKQQLSEKGYYWFNLPKTAGYPFLALVEHTNFLSSDYFGGDHIIYCGDYLDTDH
                     ENFRLSQDELLARFLPALSRVNPAFTPDWVRKSWLFRTNYAQPIPLVNHSRNIPDIQT
                     PVAGLYFASMSQVYPWDRGTNFAVQIGRQAARLMMGS"
     gene            complement(3660..4712)
                     /locus_tag="PKL11_07950"
     CDS             complement(3660..4712)
                     /locus_tag="PKL11_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ13575.1"
                     /translation="MLKRWQFWVGLLISIVFLYWSLHNLKLDEVWATLKTAKYLWLIP
                     GVAVYFMGVWVRAWRWHYLLRPLKAIPTRTMFPIVAIGYMGNNIYPARAGELLRAAIL
                     KQREGISISASLATILIERIFDGVVMLGFVFFNLPELAKLTGASGFVGNIQQLSIWGA
                     VIFAAALLAFLLAAMFPRPAERLMNWFVDRLVPQRWREKVREFGLRFLGGLESLRSPR
                     DALMVLITTTIVWLFETGKYWFVMHAFPFEVSFFALMLMNGIVNLATTLPSAPGYVGT
                     FDAPGIALLTAYNVPGELAAGYTLVLHGALWFPITLLGAYYYLRQPLRWGQDLQKLRK
                     EEDGQPTAPASPSEVK"
     gene            complement(4932..5660)
                     /locus_tag="PKL11_07955"
     CDS             complement(4932..5660)
                     /locus_tag="PKL11_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ13576.1"
                     /translation="MKVIGLHSFRRGVGKSTLAANLAYLLAAAGKRTILMDVNLYSPA
                     LEIFFKLKRDLSAPTLNDYLSGASPITELVCQPIPDLPLHLVPASSRTADMISALRPP
                     WDTTALLNGVAELDASLNPDYLLLDMASGLHEESILCMSVCNLILEVLRADAQNYQGT
                     AIMVGVARRLQVEPRLVLNDAPLDLDLPVVRQRLEKAYDCPVAAMFTHSDQMMALGAV
                     GGLFVRQNPDAEISQRLKELAASL"
     gene            complement(5665..6726)
                     /locus_tag="PKL11_07960"
     CDS             complement(5665..6726)
                     /locus_tag="PKL11_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNZ13577.1"
                     /translation="MVETPTQPHPKRKGFYMSLRLKLLISLTLLFTAAFAGIFYWFYN
                     FVSDQTWNLIKTDAQNTLTGALEGIDGDQFEALVQEAQPRADGYTDDPRYWEHVQWLY
                     HVHEVEPRAFVYTYVKGAKENEVLFIGSHGAAMTPPEGAPFLTSYEEPADNPLLTNGL
                     AAPVVNEFFLESSGADDFGAWGVTTCTPIKNSKGEIVGALGIDFQTDYVKKVQATLRT
                     LLLVVFGIIYVGFFLIIWFYSNSLARPITRLTQIARQIGDGHYDQDLAGLTKARLRDE
                     IGTLAEVFDIMVGKVEHREQSLRQEVQRLQIEIDESKRQNQVNEIVETEFFQELVTRA
                     QEMRDRKRARLAGELPPQE"
     gene            complement(6738..>7552)
                     /locus_tag="PKL11_07965"
     CDS             complement(6738..>7552)
                     /locus_tag="PKL11_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13578.1"
                     /translation="VQRALDSLGLRLPRPASSCLSTEKGELMAADTAQDVSVTLVDLL
                     RTPSAADVDALMALFDELFPQYAPIPYVVNELRQMTWEPADVKAPFIRHLWLVKVNDQ
                     PAGMTFFCYHKERNLAIGVYLAVRSEFRRLVINGYHRLAEYLITQTHQVIAADAQACG
                     HPVPPGYIAEVDVGRLMQQYRQYGFILLPVSYHEPRLPDGCTIPLPPDSLDYHPINLG
                     FFPRNPQAFDELPSRDLIRSGLLTFLVDFYGIPMDHWSVQQALQSIPADLPE"
BASE COUNT         1734 a         1959 c         2337 g         1522 t
ORIGIN      
        1 cgtcagtctc tgaattgatt gtaccgaagg cggtcggttt cgtcaaatct taatggtttt
       61 tatgagagaa aacctcagcg caggatggca ggcaggttga tgacccgggc aattccttca
      121 aaggcgccgg catcgcagcc gacaaaatcg cggttatagc cgcgctggtc ggccgtcaca
      181 gtgtaaccct caaaattagt gcatccactg gggttattat cactggcagg gctgccgggc
      241 agcagcccat gcgtgggggt cagtccgccc aggtaagcca ggggggtcaa caggggatcg
      301 gtattggtct ggtcgctacc agcggtaaga tgacaggtag catcgtctga aatgttatag
      361 cctgccgtat tccaggttcc gttgccgttg caattggtta cgtaatcacc gccaagtgca
      421 ttggcaacaa tggaattctt cagataaacc gcggcgccag aagaattata caggtttgca
      481 gcattattag cggcgatatt gcgggcaacg gtaacgttgg caagatacat tgccgcatcg
      541 ttgctgattc cgccgctggt taaatctgaa gtgttgccac tgattgtgtc gttgatcatc
      601 tgcatggtgc cataattgat gatgccaccg ccagcaactg tggcgtgatt gccgctgatg
      661 gtcgaattta aaagagttaa ggtataagct gaatctgtat taaaaattcc agcgccacgc
      721 gaggcccaat tgctgcgaat ggtggtttga ttgacgatta tgttggcgga tatgttaaac
      781 aacccacccc ccgaggcctg tgaacagtta tcggtgccgg gcaggcaaac caccgaatta
      841 ttttcaatga tgaccgaatc cagtgtcagg tcggagccat cattgtagat gccagcgccg
      901 taggcaaagg ggtcagtggg gatttcggta tgaccgccgg taatcgtcag attacgcaga
      961 tccacccagg ccgtattgcg aatatgaaaa actcgatcca gtccgttggc gtcaatcacc
     1021 gtagaggcgg cgctttcgcc ctcaatgatg atctgggcat cagtctgata aatatccagg
     1081 tcgccagatt cattgtcatt ctcgttgcgc ccgtccagcg tcaacgtgta atgcccgggg
     1141 gggagatgga tatagactgc gtcgtcactg agattccagt aattcgcttc atccagggct
     1201 gcgcgcagcg tgcactgcgg accgggggta ttataatcgg catcacagac actgttaatc
     1261 ccataatcac ggatgtcacg ggggtcgttg acgtaaatgt cccaggtggt accctgggct
     1321 aaaaccggcg ccggggccgg cgccatcagg gcaatcaaaa cgattgccag caaaaggggg
     1381 acaaggttgc ttttttgcat gaaaattcct ccaggaaaaa taaaacgaca agctcgattt
     1441 tactccaatt atggcgggaa catctgattt caatatcggt ttggcgttca ctgcggtata
     1501 cttgcaccat gagcctgatc cgatgccgct ttgtgctgtg ctttttaacc cgcgccgacc
     1561 aggtgttgat gctgctgcgc aaccgcccac ccaaccaggg gctgtggaac ggggttggcg
     1621 ggcatattga accgggggaa gcgccgctgg cagcctgtct gcgagaagta cgtgaagaga
     1681 cgggttacac cctgccaaca gtgcgctttg gcggcgtgct gacctgggaa gggtttgaga
     1741 ttgagcccgg cgggctggtg ctgttcacgg ctgaggcgcc agccggggag ccagtggact
     1801 gcagcgaagg actgttggcc tggcagccgc aggactgggc ctgcacggcg ccccaggtgg
     1861 tgggcaattt gcatgtggtg ctgccctgct tgctgcgcgg cgagccgccg cagacctatc
     1921 acttccggta ttcggatggg cagatgctgg gctgggcaca gggcccggcg cccgctgaca
     1981 tgatgacggt tgaaatcaaa ggagcataac ccccatggaa gactttacct gggcgcggga
     2041 attccccggc gcaatcacgg tttgcgatac acagggtatt atcgtttata tgaatgaccg
     2101 atccgccaaa acctttgaaa aggacggcgg cgcggcactg gtgggacaga gcttgatgga
     2161 ctgccactct gcccactcac aggagatgat ccggcagatg ctggagacgc atgagcgcaa
     2221 tgtgtacacc attgagaaga atggtgtacg caagttgatt taccagtcac cctggtacca
     2281 gaacggcgaa tttgccgggc tggtggaaat ctcgctggag attccggagc agatgccgca
     2341 cttcgtgcgg ggttagctgc ccatcatcag gcgggccgcc tggcggccaa tttgcacggc
     2401 aaagttagta ccgcgatccc agggatagac ctggctcatg ctggcaaaat agagaccggc
     2461 taccggcgtt tggatgtctg ggatgttgcg cgagtgattg accagcggga tgggctgggc
     2521 ataattggtg cggaacagcc aggacttgcg cacccagtcg ggggtgaagg ccgggttcac
     2581 gcggctgagc gccggcagga agcgcgccag cagttcatct tgactgaggc ggaagttttc
     2641 gtgatcggtg tcgagataat caccgcagta gatgatgtgg tcgccgccga agtaatcgga
     2701 ggagaggaag ttggtgtgtt ccaccagcgc caggaaggga tagcccgccg tcttgggcag
     2761 gttgaaccag tagtaaccct tctcggagag ctgctgcttg attgccagta ccatcaccac
     2821 ggcgcccatg cttttgaggt tgagcagccc ctgcaaatac tcacgcggga gggcgggcgt
     2881 caggcgcgcc atcagcgcag gtgaggtggt ggagaggatc cggtcaaagg tcagcggctc
     2941 ggcgccgggc aggcgcaggc acagcccgcc ttcagccagg ggttcaattt gctgcaccgg
     3001 ggtattgagg tggatttcag ccccgcggga gcgcaaattc tcggcaaaac gatctaaaaa
     3061 ggcctgaaag ccgccaatga aggtgcccag ccgggtggag cgggtcttga agcgcgccca
     3121 gaaccaggcc atgttcactt ctttataatg cgggccaaac ttgccgatca gcatgggctc
     3181 aaactgggtg gcatagagcc gctcgccgta atagcggcgc atccattcgt gggcggtgac
     3241 tttttccaga ggctgccagg ccgccagata gcgcagatag gccgtgacaa agccaaagcg
     3301 ggccatatct atcagggtga agccggggaa ggtcagtgcg gccaggggtg aatcgagagg
     3361 ataaaaatca tccttgtaat acaccaccgt cttggggcgc gggaagagca catcatcctg
     3421 ccagcccagc tcacggatca ggcccagcat ctcggcatcg gtctggaacc agtgatggta
     3481 gaactgctcc accgaccaat cccagtgcgg ttctttgaac ccggcagcca gcccgccgac
     3541 atggtcggcg gcttcaaaaa tagttaccga gtgcccggct ttgagcaaat cgtgggcggc
     3601 agacaggccg ctgattccgg ctccgatgat ggcaatgcgc atggaaaact cctgtttggt
     3661 tatttcactt cagatggtga tgctggcgca gtgggctgcc catcttcttc tttacgcagc
     3721 ttttgcaggt cctgacccca gcgcaagggc tggcgcaggt agtagtaagc gcccaggagg
     3781 gtaatgggga accacagggc gccgtgcagc accagggtat agccggcagc cagctcgccg
     3841 gggacattgt aggcagtgag cagggcaatg ccgggggcat caaacgtgcc aacgtagccc
     3901 ggcgctgaag gcagcgtggt cgccaggttg acgatcccgt tcatcagcat gagagcaaag
     3961 aacgagactt caaacgggaa ggcgtgcatc acaaaccagt atttgcctgt ttcaaacagc
     4021 cagacgatgg tggtggtgat cagaaccatc agggcatcgc ggggagagcg cagtgactcc
     4081 agtccgccaa ggaagcgcag gccaaactca cgcactttct cacgccagcg ctgcggcacc
     4141 agccggtcga caaaccagtt catcaggcgc tctgcgggac gggggaacat ggcagccagc
     4201 aagaaggcca gcagggccgc cgcaaagatg accgcgcccc agatagaaag ctgctggata
     4261 ttgccgacaa agcccgaggc gccggtcaat ttggccagtt caggcaggtt gaagaagaca
     4321 aagcccagca tcaccacgcc gtcgaagatg cgctcgatca gaatggtggc cagtgaggcg
     4381 gaaatggaga tgccttcacg ctgtttgagg atggcggcgc gcagcagttc gccggcgcgc
     4441 gcggggtaga tgttattgcc catgtagccg atggccacaa tggggaacat ggtgcgggtg
     4501 ggaatggcct tgagcgggcg cagtagataa tgccagcgcc aggcccgcac ccagaccccc
     4561 ataaaataga ccgccacacc cgggatcagc cagaggtatt tggctgtctt caaagtcgcc
     4621 cagacttcat caagcttcag gttatggagc gaccagtata gaaaaacaat gctgatgagc
     4681 aagccaaccc aaaattgcca gcgtttgagc atggagtatt cgcctcaggg tagtagagtg
     4741 ttcctattgt aacacagaca gattggacgc atttgattgt aagacaaggg tattaaatgg
     4801 gcaagcgcct gagtgagggg ggcacccaag cgcttgctta tttgtattct agcatatccc
     4861 cggattttat acaagatggt ttttgctgta tttaaagatt ttacaaaaag cacaggaaac
     4921 gaccagccgt gctaaaggct ggcagccaat tccttaagac gctggcttat ttcggcatcg
     4981 gggttctggc ggacaaacaa accgccaacg gcgcccaatg ccatcatctg atcggagtgg
     5041 gtgaacatgg ccgcgaccgg gcagtcgtag gctttttcga gcctttgccg aacaaccggc
     5101 aggtccaggt cgaggggggc gtcattgagc accaggcggg gctccacctg caggcggcgg
     5161 gccacaccca ccatgatggc ggtgccctgg tagttctggg catcggcgcg caacacctca
     5221 aggatcaggt tacagacgga catgcacaga atcgattctt cgtgcaggcc cgaggccata
     5281 tccagcagca aataatccgg gttcaggctg gcatcaagct cggctacgcc gttcagcagc
     5341 gcggtggtat cccacggcgg gcggagggca gagatcatat cagcagtgcg gctgctggcc
     5401 ggaacaaggt gcagcggcag gtcgggaatg ggctgacaaa ccagctcggt gatggggctg
     5461 gcgccgctca ggtagtcgtt gagggtgggg gcagacaggt cgcgctttaa cttgaagaag
     5521 atctccagcg cgggcgaata gaggttcaca tccatcagga tggtgcgctt tccggcagca
     5581 gccagcaggt aagccaggtt ggctgccagg gtggatttgc ccaccccgcg gcggaatgaa
     5641 tgaaggccga tgactttcat ccggttactc ctgaggcggc agttcacccg ccaggcgggc
     5701 acgtttgcga tcacgcatct cctgggcgcg ggttaccagt tcctggaaga attcggtttc
     5761 gacaatttcg ttcacctgat tctggcgttt ggattcgtca atttcaattt gcaggcgctg
     5821 aacttcctgg cgcagcgatt gttcacgatg ctcgaccttg ccaaccatga tatcgaacac
     5881 ttcggccaaa gtgccgattt catcgcgcag gcgagccttg gtcaggccag ccaagtcctg
     5941 gtcatagtga ccatccccaa tctgacgggc aatctgcgtg aggcgggtga tgggacgcgc
     6001 caggctgttg gagtagaacc agatgatcag gaaaaagccg acatagatga tcccaaaaac
     6061 caccagcagc agggtgcgca gggtggcctg cacctttttg acatagtcgg tctggaaatc
     6121 aatgccgagg gcgcccacaa tttcaccttt ggaatttttg atgggcgtac aggtggtgac
     6181 accccaggcg ccaaaatcat cagccccgga ggattccagg aagaattcat tgaccaccgg
     6241 agccgccaga ccattggtca acaaagggtt gtcggctggt tcttcgtaag aagtcaggaa
     6301 tggggcgcct tcgggaggcg tcatggctgc gccatggctg ccaataaaga gtacctcgtt
     6361 ttctttggcg cctttgacat aagtatagac aaaggcgcgc ggttccactt catggacatg
     6421 atagagccac tgcacatgct cccagtagcg cggatcgtcg gtgtaaccat cggcacgcgg
     6481 ctgggcttcc tggaccaggg cctcgaactg atcgccatct atgccttcca gagcgccggt
     6541 caaggtgttc tgcgcatcgg tcttaatcag gttccaggtc tggtctgaga caaagttata
     6601 gaaccagtag aaaataccgg caaaggcggc agtgaacaac agcgtcaggc tgatgagcag
     6661 cttgagccgc aggctcatgt aaaaaccttt acgcttgggg tggggctggg tgggggtttc
     6721 gaccatgatc ataactccta ttcgggaaga tccgccggga tggattgcaa ggcctgctga
     6781 actgaccagt gatccatggg gataccataa aaatcaacca ggaaggtcaa caggcccgag
     6841 cggatcaagt cgcgcgaggg tagttcatca aatgcctggg ggttgcgggg gaaaaagccc
     6901 aggttgatgg ggtgataatc caacgagtcg ggtggcaatg gaatggtgca gccatcgggc
     6961 aggcgcggct cgtggtaact gaccggcagg aggatgaagc catactggcg gtactgctgc
     7021 ataaggcggc caacatccac ctcggcgatg taaccaggcg gcacaggatg gccgcaagcc
     7081 tgagcatctg cggcaatgac ctggtgcgtc tgagtaatga gatattccgc caggcggtga
     7141 tagccattga tcaccaggcg gcggaattct gagcgcacag ccagatacac gccaattgcc
     7201 aggttgcgtt ctttgtggta gcagaaaaag gtcatgccgg cgggttggtc attcacttta
     7261 accagccaca gatgccggat aaagggggct ttaacatccg ccggttccca ggtcatttgt
     7321 cttagttcat tgaccacata gggaatgggc gcatactgtg gaaacagctc atcaaagagt
     7381 gccatcagtg cgtccacgtc tgcggcagac ggggtgcgca gcaaatcaac cagggtcacc
     7441 gatacgtctt gagcagtatc agcagccatt aattccccct tctctgtaga gaggcaactt
     7501 gatgccgggc gcggcaggcg caagcccagc gaatcgaggg cgcgctgcac gg
//
LOCUS       DAOPVS010000094         7506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_9827_length_7506_cov_43.354852, whole genome shotgun sequence.
ACCESSION   DAOPVS010000094 DAOPVS010000000
VERSION     DAOPVS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9827_length_7506_cov_43.354852"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1448)
                     /locus_tag="PKL11_07970"
     CDS             complement(81..1448)
                     /locus_tag="PKL11_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HNZ13579.1"
                     /translation="MARQISRVRRDPLHPRWHFAPPANWLNDPNGLIYWQGRYHLFYQ
                     HNPFEPAWGSIHWGHAASTDLLHWKHLPIALAPDPAGSDAGGCFSGCAVDHNGLPTLL
                     YTGVYPEVQCLATSPSGDLLKWHKRPAPVIPAPPAGLSLEGFRDPCVWSENSEWRMAL
                     GSGITGVGGAVLLYRSSDLIQWDYLGILYQGDASIRTPIWTGTMWECPAFFPLGEKWV
                     LVISVTAAEGGLYTLYYTGDYRANQFIPDSSPRLLDYGAGGCYYAPQTFLDARGRRLI
                     FGWLREARPAADQVRAGWSGALSLPGVLSLSTDGALHCAPWEAIASLRRIHQTLSSNQ
                     LSTQVQGSSMELLIRVDISSPVWSGVALLADSKKQAQIGYDPLQGAIILDCRPAGGLI
                     TTFPFPTGLADLANLHIFIDGSVIEVFDDNRLLASARFYPQNPHAMIAQCVGCASADI
                     WQLEP"
     gene            complement(1694..3001)
                     /locus_tag="PKL11_07975"
     CDS             complement(1694..3001)
                     /locus_tag="PKL11_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13580.1"
                     /translation="MHILNLLGPLAWDCFPERDLQRHYGHPSTPYAAVAAACLLKLDL
                     GFGSCARLTDWLAENPSIAWLLGFRPNTDAFNPAWNLDPAKLPDQRHFSRLLHKAPNS
                     CFQYLLSSSIQLLLDEFARRQIVVPDIVSMDTKHILAWVKENNPKAYVEERFDKTRQP
                     SGDQDCKLGCKRRHNQCKAADDALPSATPASNPLPGSSVSIGEYYWGYASGVAAAKVP
                     EWGEFILAEMTQPFNCSDVSYFFPLMTVVEQRLGRKPRFGTLDAAFDAFYIYDYFYQA
                     GGFAAVPKAEKGKIAHRDFDPAGLPLCAAGLAMPLKMTYWDRSTAIIEYERGQYACPL
                     FHPMLTDSPCPINDEHWATGGCHTTLATSIGARIRHQLDRDGVDYKQIYKQRTAVERI
                     FSQAVELGIERPHLRNQWAIANINTLIYTLINLRLLRRIQQRA"
     gene            complement(3364..3669)
                     /locus_tag="PKL11_07980"
     CDS             complement(3364..3669)
                     /locus_tag="PKL11_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13581.1"
                     /translation="MPAIPEFLLRKLFVPNSLKPTTNGFRFELVNTFAPVALLNLTLS
                     ADDIACPVATITIVMPQQPPFHASEITPAHPFVLPMNIAVEIQIQFPVPQKRVSSVK"
     gene            complement(3676..4974)
                     /locus_tag="PKL11_07985"
     CDS             complement(3676..4974)
                     /locus_tag="PKL11_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13582.1"
                     /translation="MTLSSTPLKQQSPLSRVVWAMLLLLGLAGQLAWAVENQYFNTFM
                     YDNITPDPRAVSWMVAITAVVSTVATILMGTLSDRTRTRWGRRRPYLFIGYIVWGILT
                     AAFPLAARFHPIAVGVFIAILFDSIMSFFAATASDGAFSAYVTDITTEVNRGRVVGSL
                     EIMKWVAFLIIYGGAGFIIQAVGYYWFFYIIGGIAALIGLVCVPFLREEPLTDKPQGT
                     YWGQIANTFQLSSLRQNRDFFKVMIALTLFMVGINVFFPYLMIYLQHYIQLSIVNSSI
                     LVAISILVGGIGLAYPIGLLVDRWGRRPVALVAVGLEAVGLLVFSLSRSMVMLTIGGI
                     LWLAPFAAWTIATTTWSRDLYPEDKRGQFAGYYVLFNVAFTMIPGPLLGGWLASTYGI
                     ATVLDGKPGFIPTPILFQVAAVLVLLAVIPLLTIRMPKTK"
     gene            5173..6156
                     /gene="mvaD"
                     /locus_tag="PKL11_07990"
     CDS             5173..6156
                     /gene="mvaD"
                     /locus_tag="PKL11_07990"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNZ13583.1"
                     /translation="MTALTATALAHPNIAFIKYWGNRDQVLRLPVNGSISMNLEGLFT
                     RTRVSFDEDLDADILILNGQDVVGSALSRVSALLDQVRGMAGKKLFAQVESSNNFPMG
                     AGIASSAAAFAALSLAASRALGLELHEAELSRLARLGSGSACRSIPGGFVEWQTGQGD
                     ADSFAVSIAPAEHWKLVDCVAVIETGHKPVGSTQGHALADSSPLQAARVADAGRRLNR
                     CRNAILKRDFRALAEVVELDSNLMHAVMMTSTPPLFYWEPASVQLMKAIPAWRSEGLP
                     MCYTLDAGANVHAICPAEAAGQVAEQLSRQPGVQQVLQATVGGPARVEDIS"
     gene            6153..6719
                     /locus_tag="PKL11_07995"
     CDS             6153..6719
                     /locus_tag="PKL11_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13584.1"
                     /translation="MKFFPQNMTLMLLIATILCSCINPTAITETPETSILQTMVPTDD
                     ISIAPTPTKTPQEITAYYDGVVVLTQYYTFLGNGLHENAYYLMSDSAQKQYLSFEHYL
                     EMAQISFHQIEIVQIIPYKDWAPLFGLRYFEDTADEKQFVVQIKAWGQGTMSGSAFNG
                     QLQTLHVMLIFEDGLWKINEFGTMAFNP"
     gene            6861..>7506
                     /locus_tag="PKL11_08000"
     CDS             6861..>7506
                     /locus_tag="PKL11_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNZ13585.1"
                     /translation="MTSLLRILEFILALGVLVFLHELGHFLVSRFFKIEIEEFGFGFP
                     PRMVKLFKLGGTDFTINWIPFGAFVRPKGENDPEVPGGLASASPWVRLAVLLGGPVMN
                     LLTGVILFTIMFSRGSYYDITRVEIAEVAAASPAEQAGLLPGDLITAIDGKRVDGSSR
                     LQTLVLARAGQEITLTVERDGEKLTFTTTPRVSPPEGQGALGIVMTSPIVENQTF"
BASE COUNT         1911 a         1815 c         2215 g         1565 t
ORIGIN      
        1 aatttttatg aataacataa atccggtgca gtcagggtcc gactgcaccg gattggttta
       61 atgagcggtt gtgggaatga ttatggctct aactgccaaa tatctgccga ggcgcagcct
      121 acacattgag caatcatggc atggggattt tgcggataga accgcgcaga cgcaagcaga
      181 cgattgtcgt caaaaacctc gatgacagaa ccgtcgataa aaatatgcag gttcgccaaa
      241 tcagccaacc cggtggggaa tgggaatgtg gtaatcaggc cgccggcggg gcggcagtcc
      301 aagataattg cgccttgaag cggatcatac ccaatttggg cttgtttttt agaatctgcc
      361 agcagggcga cgccgctcca gacgggactg gagatatcca ctcttatcag caactccata
      421 gaagaaccct ggacctgggt ggataactgg ttcgaggaaa gggtttggtg tattctgcgg
      481 agcgaggcaa tagcttccca tggcgcacag tggagcgcgc catccgtgct gagcgacagg
      541 acaccgggca gggagagtgc gccgctccag cctgcacgga cttgatcagc ggcggggcgg
      601 gcttcacgca gccagccaaa aatcaaacgg cgtccccggg catccaaaaa tgtctgcggg
      661 gcataataac agccgccagc gccgtaatcc agcagccgcg gggaggaatc ggggataaac
      721 tggttggccc ggtagtcgcc ggtgtaatac agcgtgtaca acccgccctc ggcagcggta
      781 acggaaatca ccagcaccca tttttctccc aggggaaaga aggcgggaca ttcccacatt
      841 gtgcccgtcc agattggggt gcggatagag gcatcgccct ggtacaaaat gccgagataa
      901 tcccattgga ttaaatcgga ggaacgatac agaaggacgg caccgcccac gccggtaatg
      961 cctgaaccaa gcgccatccg ccactcggaa ttctcagacc acacacaggg gtctcgaaag
     1021 ccttccagag acagaccggc aggcggggca gggataaccg gtgccgggcg cttatgccac
     1081 ttcaaaagat cgccggatgg gctggttgcc aggcactgca cttcaggata gacgccggtg
     1141 taaagcaagg tgggcaggcc gttgtgatct acagcacagc cggagaaaca accgcccgca
     1201 tccgaacctg cgggatcagg cgccagggcg atgggcaggt gcttccagtg aagcagatct
     1261 gttgaggcgg catggcccca gtgaatgctg ccccaggcag gttcaaaggg attgtgctgg
     1321 tagaagaggt ggtaacgacc ctgccaatag attaaaccat tcgggtcatt cagccaattg
     1381 gctggggggg caaaatgcca gcgcgggtga agcggatcac gccggacgcg ggagatctga
     1441 cgcgccaatc gcaggcgctg ccgaagaccc gcgaaaatag gccggcgcgg tttcgggtta
     1501 tgcgcctcac ccttgagcgg gatgctgaac tggaggatgc ccgcctcgcg ggtttcagcg
     1561 cgaatcgtta acttcttctg agagggttag ctcaatcagg actggttacg tcactggtta
     1621 cggttttgta aaaaaagcgt aaccagtcaa cgggactggt tacgttatca catccgttct
     1681 ttaacaatca aggtcaggcg cgctgttgaa tacgccggag caaccgcaag ttgatcaggg
     1741 tatagatgag cgtattgata ttggcaatag cccattgatt acgcaaatga gggcgttcaa
     1801 tgcccagttc tacagcctgg ctgaaaatgc gttcaaccgc ggtacgttgt ttgtagattt
     1861 gtttgtaatc aaccccatcc cgatccaact ggtggcgaat acgagccccg atgctggtag
     1921 ccagggttgt gtgacaacca ccggttgccc aatgctcatc attgatcggg cagggcgagt
     1981 cggtgagcat gggatggaat aggggacagg catactggcc gcgttcgtat tcgataattg
     2041 ccgtgctgcg gtcccagtaa gtcattttga gcggcatcgc taaaccggcc gcacacaaag
     2101 gaaggccagc aggatcaaag tctcgatgcg cgattttgcc cttctcggct ttgggaacgg
     2161 cagcgaaccc ccccgcctgg tagaagtaat cgtagatgta aaaagcgtca aaagcggcat
     2221 ccagcgttcc aaaacgaggt ttccggccca ggcgctgctc caccacagtc atcaaaggga
     2281 aaaagtagga gacatccgag cagttgaacg gctgggtcat ctctgccagg ataaactcac
     2341 cccattctgg aactttggcg gcagccacgc cggaagcgta gccccagtag tattctccga
     2401 tggacacaga actgcccggt aatggattgc tggccggagt ggcagatggc agagcatcgt
     2461 cagcagcctt gcactggtta tggcggcgct tgcagcccaa tttgcagtcc tggtcaccag
     2521 acggttggcg ggtcttatcg aaccgttctt caacataggc ctttggattg ttttctttga
     2581 cccaggccaa gatatgcttg gtatccattg atacgatatc tggcaccaca atctggcgac
     2641 gggcaaactc atccaacagc agttggatgc tgctggatag taggtactgg aagcaggaat
     2701 taggcgcttt gtggagcagc cgcgaaaagt gtcgctgatc aggcagcttt gcgggatcga
     2761 gattccaggc gggattgaaa gcatccgtat taggacggaa tcccagcagc caggctattg
     2821 aggggttttc agccagccag tctgtcaggc gggcacagga gccaaaaccc aggtccaact
     2881 taagcaggca ggctgccgca accgctgcat acggggtgct gggatgtcca taatgacgct
     2941 gcaagtcgcg ctctggaaaa caatcccagg ccaaaggccc caacagattg aggatatgca
     3001 tggccgtggg atccttgcgg acgctcggag gcaattgttc tggatgacag aacagactgt
     3061 gtaaatcttg aggcgacggg ggccgaaaac ggcgcacaaa cccgctgaaa cgggatacaa
     3121 tagggttgtt cgacatgatt ttctcctttt gtgagccaaa aatggatgtg acaatctcat
     3181 tttagaccca tctggagcat gccggacacg taaccagtca ccggactggt tacgttttac
     3241 aaaccaatac ttgcctgatg aaactgactg gctacaaaaa acgtcgccag tcaatggatt
     3301 ggttacgttt ttgatgccct ctttggcgac tggttacgca ctgggcacga aaaccgtaac
     3361 cagttatttc actgagctaa ccctcttctg aggaactgga aactggatct gaatctccac
     3421 cgcgatattc atgggtaaaa caaatgggtg tgcgggagtg atttctgagg cgtgaaaggg
     3481 aggctgctgc ggcattacaa tggtgatggt agcaaccggg caagcaatat catcagcaga
     3541 cagggtcagg ttgagcagtg caaccggggc aaaagtattt accagttcaa atctgaaacc
     3601 attggtggtt ggcttcaggc tgttgggaac gaatagttta cggagtaaaa attcagggat
     3661 ggcgggcatg acaggttatt tggttttggg catgcgaatg gttaacagtg ggatcacagc
     3721 cagcagcacc agcaccgcag ctacctggaa gagaatcgga gtcgggataa agccgggttt
     3781 gccatccaga accgtggcga tgccataggt tgaggccagc cagccgccca gcagagggcc
     3841 ggggatcatg gtaaaagcca cattgaacag gacataatac ccggcaaatt ggccgcgctt
     3901 atcttccggg tacaggtcac gcgaccaggt ggtggtcgca attgtccagg cggcaaaagg
     3961 agccagccag aggatgccgc caatggtaag catgaccatc gagcgcgaga gggagaaaac
     4021 cagcaagccc acagcctcca ggccaacagc caccaaggcc accgggcgcc gcccccagcg
     4081 atcaaccagc agaccaatcg ggtatgccag gccaatgccg ccaaccagga ttgagatggc
     4141 caccaggatg gaggaattga caatggaaag ctggatatag tgctggagat aaatcatcag
     4201 gtaagggaaa aagacattga tgcccaccat aaagagggtc aatgcaatca ttactttgaa
     4261 gaagtcgcgg ttctggcgca ggctggatag ctggaaggtg ttggcaatct gcccccaata
     4321 ggtgccctgg ggtttatcgg tcaggggttc ttcacgcaaa aagggcacac acaccagacc
     4381 aatcagcgcg gcaatgccgc caatgatata aaagaaccag taataaccca ctgcttgaat
     4441 gataaaacca gcgccgccat agataatcaa aaaggccacc cacttcataa tttcgaggga
     4501 gccgacaacc cggccgcgat tgacttcggt agtgatatcg gtgacgtagg cgctgaaagc
     4561 gccatcggag gctgtggcgg caaaaaaaga catgatgctg tcaaataaaa tggcgataaa
     4621 aacacctacg gcaatgggat ggaacctggc cgccagcgga aaagcagccg ttaaaatgcc
     4681 ccaaacaatg tagccgatga acaggtaagg gcggcggcgc ccccaacggg tgcgagtgcg
     4741 atcgctcaac gtacccatca agatggttgc gacggtggaa acaaccgctg taattgcaac
     4801 catccaggaa acagcccggg ggtcgggggt gatgttatcg tacataaagg tgttgaagta
     4861 ttgattttca accgcccagg ctaactgccc ggcaaggcca aggagcagca acattgccca
     4921 gacgacgcga ctaaggggtg actgctgttt aagcggggta ctggataggg tcatggaggg
     4981 ttcctgtgac gacgggatgg cttaagtata gcgtaaatac agcctggagg cttactgaag
     5041 catattcgct tttgggggaa tatccaaaag ctctataaat tccaaggtca tgaccgataa
     5101 aagaaggaca aaacagatat caaaacgcga ggcacaaact ttgtgataaa ctaattcagg
     5161 aaaggagcaa aaatgactgc acttactgca acggccctcg cccaccctaa tattgcgttc
     5221 atcaaatact gggggaaccg agatcaggtg ctccggctgc cagtgaatgg ttcgatttcg
     5281 atgaacctgg aggggttatt tacccggacc cgagtgagct ttgacgagga cctggatgcc
     5341 gatattttga ttttgaatgg gcaggatgtg gtgggcagcg cgttgagccg ggtgagtgcc
     5401 ctgctggatc aggtgcgggg gatggccggg aagaaattat ttgcgcaggt ggagagcagc
     5461 aataatttcc caatgggcgc ggggattgca tcttcagcgg cagcatttgc ggctttgagc
     5521 ctggcggcct ctcgggcgct tgggctggaa ttgcacgagg ctgagctttc acggctggcc
     5581 cggctggggt cagggtcggc gtgccgctcg attccgggag gattcgtgga gtggcagacc
     5641 ggacaggggg atgctgattc gtttgcggtt tcaattgcgc cagcagagca ctggaagctg
     5701 gtggactgcg tggcggtaat tgagacagga cacaagccag tgggatcaac ccaggggcat
     5761 gcgctggcag acagcagccc attgcaggcg gctcgggtgg ccgacgccgg aaggcggctg
     5821 aaccgctgcc gaaacgccat cttgaagcgg gatttcaggg cactggcaga ggtggtggaa
     5881 ctggacagca acctgatgca cgctgtgatg atgacctcaa caccgccgct gttttactgg
     5941 gagccggcct cagtgcaatt gatgaaggct attccagcct ggcggagcga ggggctgccg
     6001 atgtgctata cgttggatgc gggagcaaat gttcacgcga tttgcccggc agaagcagcc
     6061 ggacaggttg cagagcaatt gagccgacaa cccggggtac aacaggtttt gcaggcaacc
     6121 gtaggcgggc cggcgagagt ggaagatata tcatgaagtt tttccctcaa aacatgaccc
     6181 tcatgctact tattgcaaca atcttatgct cttgtatcaa tccaacggcc atcacggaga
     6241 cgccagaaac ctcaatatta caaacaatgg ttccaacgga tgatatatct attgcgccta
     6301 cacccacaaa gacgccacag gaaattaccg cttattacga tggggtggtt gttttaactc
     6361 aatactatac ctttctggga aatgggctgc atgagaacgc ctattatttg atgagtgatt
     6421 ccgcacaaaa gcaatatctg tcattcgagc attacctgga aatggcacag atcagctttc
     6481 accaaattga gattgtccaa attattccat acaaagattg ggcccctctt tttggattaa
     6541 gatactttga agacacagcg gacgagaaac aatttgttgt acaaattaaa gcatggggac
     6601 aaggaactat gtccggttca gctttcaatg gacagctaca aacccttcat gttatgctga
     6661 tttttgaaga tgggctatgg aagataaatg agtttggtac gatggcattt aatccctaaa
     6721 cgttgtatcc attgtggaat gaattattcg ttcccagatt acacaacacg ctacaaggca
     6781 ccggttaaag cgttataatt aggaaaattc atcaaaaacg ggcggcatcg cccggtggtt
     6841 aaaggactat ggatactctt ttgacaagct tactgcggat tttagagttt attctggcgc
     6901 tgggggtgct ggtatttctg cacgaactgg ggcatttcct ggtttcgcgg ttcttcaaga
     6961 ttgagattga agaatttggt tttggttttc caccccggat ggtcaagctg tttaagctgg
     7021 gggggaccga cttcacgatt aactggattc cattcggggc gtttgtgcgt cccaaaggtg
     7081 agaacgaccc cgaggtaccg ggcgggctgg cttcagcaag tccgtgggtg cggctggctg
     7141 tgctgctggg tggacctgta atgaacttgc tgacgggcgt gattcttttt accatcatgt
     7201 tcagccgagg cagttactat gatattaccc gggttgagat tgcggaggtt gcggcggctt
     7261 caccggcaga acaggccgga ctgctgcccg gagacctgat tactgcgatt gacggcaagc
     7321 gagttgatgg ctcgtctcgg ctgcaaacgc tggtgctggc gcgagcgggt caagaaatta
     7381 cgctgacggt ggaacgcgac ggagagaaac tcacctttac gactacgccg cgggtcagtc
     7441 cacctgaggg gcagggtgcg ctggggatcg tcatgaccag cccgattgtt gaaaatcaga
     7501 catttg
//
LOCUS       DAOPVS010000095         7336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_10105_length_7336_cov_53.675182, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000095 DAOPVS010000000
VERSION     DAOPVS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10105_length_7336_cov_53.675182"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKL11_08005"
     CDS             <1..944
                     /locus_tag="PKL11_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNZ13586.1"
                     /translation="ASRLITLMLLQRQPNQKAAELAAELGISVRTLHRYFEMLDEMGI
                     PIYAERGPYGGFSLVRGYKIPPLVLDPEEAIAVALGTSMVEALWGQIYRDPARGALAK
                     LENMLPDEQRQEVAWARRSLIATGMHRSDLNALAPALEKLRRAVREHRRADIRYHSST
                     REQPEPRAVDFYALVHRSGWWYAVGYCHLRQEMRSFRVDRMEAVQLTDQVFQIPEAFD
                     IQAYLAQEWKSQPQIEVRLRFAPEGAQIARYNRAYWNSLEEMPDGTVHVTLTAPDLNW
                     AASSILAYGPLVTVLEPAALRQMVAEWAQAIVHYYNP"
     gene            969..1610
                     /locus_tag="PKL11_08010"
     CDS             969..1610
                     /locus_tag="PKL11_08010"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNZ13587.1"
                     /translation="METLDLKKMYQSLYMPAARKIEAVEVPELQFIMIDGAIEPGQAP
                     GTSSLFEENTQALYGAAYTLKFMVKQRKVEPVDYPVMALEGLWWVEDGVFDIAVKDNW
                     VYTLMILTPDLVTPAMFAEALAQLRKKKGDQPGFARLRLERFCEGLCVQALHVGPYAT
                     EPATMTEMQAFMEANGYHDQVGLGGKHHEIYLGDPRRAAPEKLKTVLRHPVSR"
     gene            1638..2150
                     /locus_tag="PKL11_08015"
     CDS             1638..2150
                     /locus_tag="PKL11_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNZ13588.1"
                     /translation="MSRPINKAQLITQATQAYEALEKLLAGLSPEQMTVPGVLGEWSA
                     KDVLGHLYAWQQMFFRWYEAGLRGENPAVPAESYKWSQIPALNQKIYEIYRDSPLAEI
                     WVNFRVSHAKTLALIESLSDEALTTPGLYPWMNQNSLIAYLAANTSSHYTWAYTGLRK
                     GLRAKKGSGS"
     gene            complement(2184..4469)
                     /locus_tag="PKL11_08020"
     CDS             complement(2184..4469)
                     /locus_tag="PKL11_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13589.1"
                     /translation="MDQSPAPEKRKSGWFWRLVDRIRSAGRAITPGPRAWRGAAWGLI
                     IITIGLVLYTTYNAFAPFGLLAFVLGFLALLVIAVLLKGLTLLLIRLDGAVPVGYKTT
                     LVGAVPLLFVCFLMLNLGFAAVLLGIIGVSSLLGAAVWVLLRGGWRSATKAQRAVIGL
                     GLILGLVGLIGGGAWLLNDGSPVTPPQNAAAQAGAVVIPLAVEDPSQPGPYAVQTLFY
                     GSGSDQRRPEYGSQVDLVTQAVDGSKLVEDWSGLRSQYWGFGPEAMPVNGRVWFPDGP
                     GPFPLVLIVHGNHEMEAYSDPGYAYLGELLASRGFILVSVDENFLNLSPTADLLFLNG
                     LQEENDARGWLLLEHLQVWQGWNADPGSPFYQKVDLNNLALMGHSRGGEAVAVAAAFN
                     HLPFYPDDASLAFDYNFNIRSVVAIAPVDAQYQPTDQLTPLENVNYLVLHGAQDMDVA
                     SFSGSRQYERIRFTDGQFWVKAGVYIYGANHGQFNTVWGRKDGFEPGMRLMNLTQLMP
                     PDEQQQIAKVYITAFLEATLHSQTAYLPVLRDHRTAAGWLPNTIYISQYLDSTTRMLS
                     DYEEDINLATTTLPGGSLQGQNLTLWREQVVPLKWDTLNTNAAYLGWDTTADSAVPTY
                     EITLPDGSLALGDDSTLVMSLANANEPPTADAEEANTDLIDLTIEVIDRRGAAARLPL
                     SHFAYLQPQIEDHLPKASWMALPAQTSEVVFQSFEFPLSAFSTANPAFDPTNLASVRL
                     VFDRPPSGVVVLDNLGFRVGE"
     gene            complement(4630..6030)
                     /gene="arsM"
                     /locus_tag="PKL11_08025"
     CDS             complement(4630..6030)
                     /gene="arsM"
                     /locus_tag="PKL11_08025"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNZ13590.1"
                     /translation="MTTSSNPSADYFNQVAGNWDAQSAGYFREGVRDAAIARAYLHPK
                     MIVADVGAGTGFMAAGLAAQVERVHLLDGSAAMLAVARQNLASFTNLEYHVADSLALP
                     LPDESLDAVFANMYLHHCPDPLAAIREMSRLLRPGGRIIITDLDAHAHEWLKTEMSDV
                     WLGFDREQVRTWYAEAGLVNVIVDCTGETCQSESDGSAEDPAAISIFIAVGTKRIAAH
                     KAVQSSYAARALGSTSCCSDSNCCSPGVTVLDEIAEVTWDGGYSLTDKAGIPADAADF
                     SLGCGNPLAMASLQEGETVLDIGSGGGLDAFIAARRVGPTGQVIGVDMTPAMLQRARR
                     TAKKGGFDNVHFKYGHAEKLPVPDGSVDVIISNCVINLTEDKGQVFREAFRVLRTNGR
                     LEVNDVVFGGAILPAARLSTAGWSECISGALPEQEYLDLVKQAGFTQVTVRRSTSAGV
                     SDGVSVYSVQVSAVKE"
     gene            complement(6113..6409)
                     /locus_tag="PKL11_08030"
     CDS             complement(6113..6409)
                     /locus_tag="PKL11_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ13591.1"
                     /translation="MNAEQKRLARMLKALGNPIRFQIVEYLASRQVCITNDIVQALPL
                     AQSTVSQHLKVLREAGLIHGEIEGPATCYCLDSEGIQWLKDRIGNWLPECCRDK"
     gene            6681..7223
                     /locus_tag="PKL11_08035"
     CDS             6681..7223
                     /locus_tag="PKL11_08035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13592.1"
                     /translation="MTDLPVIRPATIEDTLDMAQVHVDTWRTSYVGIVPAEHLANLSY
                     ERSQAGWNEHILNQHGREYIFVAEERPGKIIGITSGGPLREAMAGFDGELYVLYVLKS
                     FQGMGYGKRLVTCVAQALISAGYRSMIIWVLKDNPACRFYERLGGQPVVEKMVEIGGK
                     LLIDLAYAWSDIRGFSEIAY"
BASE COUNT         1672 a         2028 c         2146 g         1490 t
ORIGIN      
        1 ccgccagccg actcatcaca ttaatgctgc tgcagcgcca gcccaaccag aaggctgccg
       61 aattagctgc cgagttgggc atctcagttc gcacgctgca ccgctatttt gagatgctgg
      121 acgagatggg cattcccatc tatgccgagc gcggtccgta tggcgggttc tcgctggtgc
      181 ggggctataa gataccgccg ctggtgcttg acccggaaga agccattgcc gttgccctgg
      241 ggaccagtat ggtcgaggcg ttgtgggggc agatctaccg tgacccggcc cgcggcgccc
      301 tggctaaact ggagaacatg ctgcccgatg agcagcgcca ggaagtcgcg tgggcgcggc
      361 gatcgctgat tgccacagga atgcaccgtt ctgacttgaa cgcactggcc ccggcactcg
      421 agaaactacg ccgcgccgtg cgcgagcacc gccgcgccga cattcgttac cacagcagca
      481 cccgggagca gcctgagccg cgcgcggtgg acttctacgc gctggtgcac cgctcgggct
      541 ggtggtatgc ggtggggtac tgtcatttac gccaggaaat gcgctcgttc cgggttgacc
      601 ggatggaagc agtgcagctt actgatcagg tttttcaaat tcctgaggcg tttgacattc
      661 aggcatacct ggctcaggag tggaaatctc aaccacagat tgaggtgcgg ctgcggtttg
      721 cgccggaagg cgcgcagatt gcccgataca accgggctta ctggaatagc ctggaagaaa
      781 tgcccgacgg cacggtacat gttacgctga ccgccccaga cctgaactgg gctgccagca
      841 gtatactggc atatggaccg ctggtcaccg tgctggagcc tgccgcgctg cgtcagatgg
      901 tggcagaatg ggcgcaggcg attgtccatt actacaaccc ttaaccacac aaaaaggaaa
      961 ggaaaacgat ggaaacgctc gatctcaaaa agatgtatca gtccctctat atgcctgcgg
     1021 ctcgaaaaat tgaggccgtt gaggtccctg agttgcaatt catcatgatt gatggggcca
     1081 ttgagccagg acaggcgccc ggcacgtcgt ctctatttga ggaaaatacg caagcgctct
     1141 atggagcggc atacacgctc aaattcatgg tcaaacagcg caaagtagag ccggtggact
     1201 acccggtgat ggcgcttgag ggtctgtggt gggtggagga cggggtcttt gacattgccg
     1261 tcaaggataa ctgggtttat acgctgatga ttctgacgcc cgacctggta accccggcga
     1321 tgtttgccga agcgctggcg cagctgcgca agaaaaaagg tgaccagccc ggatttgccc
     1381 gcctgcggct ggaacgcttt tgcgaagggc tgtgcgttca ggcgctgcac gttgggccct
     1441 atgccaccga gccggcaacg atgacagaga tgcaggcttt catggaggcc aacggctacc
     1501 acgatcaggt tggtctgggc gggaagcacc acgaaattta tctgggcgac ccgcgccggg
     1561 ccgcgcctga aaagctcaaa accgtgctgc gtcacccggt ttcccggtag actgcacacc
     1621 aaacatcagg agtttttatg tcccgaccga ttaacaaggc tcaattaatc acacaggcca
     1681 cccaggcata tgaagccctg gaaaaattac tggcaggact ctcccctgaa cagatgactg
     1741 tgcccggcgt gctgggagaa tggtctgcca aagatgtttt ggggcatctg tatgcatggc
     1801 agcagatgtt cttccgctgg tacgaggccg ggctgcgggg agaaaacccg gccgttccgg
     1861 ctgaaagcta caagtggagt cagatcccgg cgttgaacca aaaaatctac gaaatctacc
     1921 gcgatagtcc gctggctgag atttgggtca atttccgcgt ctcgcatgcg aaaacactgg
     1981 cgctcatcga atcgctctcc gatgaagccc tcaccacgcc agggctgtat ccatggatga
     2041 atcagaacag cctgattgct tacctggcgg ctaacaccag cagtcactac acctgggcct
     2101 ataccgggct gagaaagggg ctgcgggcaa aaaaggggag cggctcataa agttgctaga
     2161 gccatggttg ccaacgcttc gttttattct cctacccgga aacccagatt gtccagtacc
     2221 accacgccgg agggggggcg gtcaaatact aacctcacag aagccaggtt ggtcgggtca
     2281 aatgcggggt tggcggtact gaaagcggac agcgggaact caaagctctg gaagacgacc
     2341 tcggaggttt gggctggcaa ggccatccag gaggctttgg gcagatggtc ttctatctgg
     2401 ggctgtaggt acgcgaagtg acttaaaggc agccgggctg cagcaccgcg gcgatcgatt
     2461 acttcaatgg tcaggtcaat caggtcggta ttggcttctt cggcatcagc cgtgggcggt
     2521 tcgttggcat ttgccagaga catcaccagg gtgctgtcat cgccgagggc caaactgcca
     2581 tccggcagag tgatctcgta ggtgggcacg gcgctatcgg cggtggtatc ccaccccaga
     2641 taagcggcat tggtattcag ggtatcccat ttgagcggca cgacctgttc ccgccagagg
     2701 gtgagatttt gaccctgaag actgccaccc ggcagggtgg ttgtggccag gttgatgtct
     2761 tcttcataat cactgagcat ccgggtggtt gaatccaggt actggctgat gtaaatggta
     2821 ttgggcagcc agccggcggc ggtgcggtga tcacgcagca cgggcagata ggcggtctgg
     2881 ctatggagag tggcttcaag gaaggcggtg atgtacactt tggctatctg ctgctgttcg
     2941 tccgggggca tcaactgggt caaattcatc aggcgcatgc ccggctcaaa gccatcttta
     3001 cggccccaga cggtattgaa ctgcccgtga ttggcgccat agatgtatac cccggctttg
     3061 acccagaact gcccgtcggt aaagcggatt ctttcatact gcctggagcc gctgaatgaa
     3121 gccacatcca tatcctgcgc cccgtgcaga acaaggtaat tcacattttc caggggggtc
     3181 aactgatctg tcggctgata ctgggcatcg accggggcaa tggccacaac cgaacggatg
     3241 ttgaagttgt agtcaaaagc cagcgaagca tcatccgggt aaaatggcag gtggttgaag
     3301 gccgccgcaa ctgccacagc ctctccaccg cgcgaatgtc ccatcaatgc aagattgttc
     3361 agatcgactt tttgataaaa tgggctgccg ggatcagcgt tccaaccctg ccagacctgg
     3421 aggtgttcca acagcagcca gccgcgggca tcattttctt cctgcaggcc attgagaaac
     3481 agcagatctg ccgttgggga aaggtttaag aagttttcgt ccactgagac caggataaac
     3541 ccccggctgg ccagtagttc gcccaggtag gcgtagcccg ggtctgagta cgcttccatt
     3601 tcgtggttgc catgcacaat caagaccagc gggaagggac cgggaccatc cgggaaccag
     3661 acccgcccat tgaccggcat ggcctcgggg ccaaaccccc agtactggct gcgcaggccc
     3721 gaccagtctt cgaccagttt cgagccatcc acagcctggg tgaccagatc aacctgggag
     3781 ccatattccg ggcggcgctg gtcgctgccg ctgccataga acagcgtctg aacggcataa
     3841 ggccccggct gcgagggatc ttccacagca agggggatca ccacggcgcc ggcctgggcg
     3901 gccgcgtttt gcggcggcgt gaccggggag ccatcgttca acagccaggc ccctccgccg
     3961 atcagaccta ccagacccag aatcagcccc aaaccgatga cggcgcgctg ggctttggtg
     4021 gcagaacgcc agccgccgcg taacagcacc catactgccg cgcccagcag ggatgagacc
     4081 ccaataattc ccaataagac tgcagcaaaa ccgaggttga gcatgagaaa acagacgaac
     4141 aggagcggca cggcccccac cagggttgtc ttgtagccca ccggtaccgc gccatccagc
     4201 cggatcaaca gtagggtcag ccctttcagc agcacagcga tgacgaggag cgccaggaag
     4261 cccagcacaa aggccagcaa gccaaagggc gcaaaggcat tgtacgtggt gtagaggact
     4321 aagccgatgg tgatgataat caacccccag gctgcgcccc gccaggcgcg cggtcccggg
     4381 gtgatggcgc gcccggcact gcggatgcgg tcaacaagcc gccagaacca gccagatttt
     4441 cgtttttcgg gggcagggga ttgatccatg aaagactcct tgaagaggga taacgttagt
     4501 tatacgcaaa attacctgtt aagttgcgaa gagccaatca tatttctggt gatagggcaa
     4561 ccccggcatc tgcgccgggg ttgctgtatc tgatcccggc accgccgaga aatcagggaa
     4621 ccgccaagat tattccttaa cagccgacac ctgtacagag taaaccgaaa cgccgtcaga
     4681 tactccggcg ctggtgctgc ggcggaccgt cacctgcgtg aatccggcct gcttgaccag
     4741 atccagatat tcctgctcgg gcagcgcgcc agagatgcac tccgaccagc cggcggtaga
     4801 caggcgcgcc gcaggtaaaa ttgcacctcc aaagaccaca tcattgacct ccaggcggcc
     4861 atttgttctc aatacccgaa aagcctcgcg aaatacctgg cccttatcct cggtcaggtt
     4921 gatcacacag ttggagatga tgacatccac cgatccgtct gggacgggca gcttttcagc
     4981 atgaccatac ttgaaatgaa cattgtcaaa accgcctttt ttagcggtgc ggcgagcgcg
     5041 ttggagcatg gccggggtca tatccacccc aatcacctgc ccggttggcc ccacgcgccg
     5101 ggcagcaata aaagcatcca gtccgccgcc agagccaata tccagcacgg tttcgccctc
     5161 ctgcaggctg gccatggcca gcgggttacc gcagcccaat gaaaaatcag ccgcatccgc
     5221 aggaatgccc gctttatcag tcagggaata gccgccatcc caggtaacct cggcaatttc
     5281 gtccagcaca gttacaccgg gcgaacagca gttggaatca ctgcagcagg atgtgctgcc
     5341 cagcgcccgg gccgcatagc tgctctggac ggccttgtgg gcggcaatgc gctttgtgcc
     5401 cacggcaata aagatgctga tggcagccgg atcttcagca gaaccgtcag attcagattg
     5461 gcacgtttct cctgtgcaat ccacaatcac attgaccagc ccggcttccg cataccaggt
     5521 gcgaacctgc tcacgatcaa aacccagcca gacatcggac atttcggttt tcaaccactc
     5581 gtgggcatgg gcatccagat ctgtgatgat gatgcggccg cccgggcgca gcaggcggga
     5641 catttcccgg atggctgcaa gcgggtcagg gcagtgatgc agatacatat tggcaaatac
     5701 cgcatccagg ctttcgtcag gcagcggcag tgcaaggctg tcggcaacgt ggtattccag
     5761 gttggtgaat gacgccaggt tctggcgtgc aacggccagc atggcagccg aaccatccag
     5821 caaatgcact cgctcaacct gtgcagccag tccggcagcc atgaaaccgg tacctgcccc
     5881 gacatcggcc acaatcattt tgggatgcag gtaagctctg gctatcgcgg catcccgcac
     5941 gccttcacgg aaatacccgg cgctctgggc gtcccagttt ccggcaacct ggttaaaata
     6001 atcggccgat ggattggatg atgttgtcat tggttctcct ttatcgtaaa aatacgatgc
     6061 ttttgcgctc aaaaaaacgc tgttaccagc gttcatggcg ggttgaagag tgctacttat
     6121 cccggcagca ttccggcagc cagttaccga tccggtcttt gagccactgg attccttcag
     6181 aatcaaggca gtagcaggta gccgggcctt cgatctcgcc gtggatcagt ccagcctcac
     6241 gcaggacttt aaggtgctgg ctaacagtgg actgagccag cggcagggcc tgcacaatat
     6301 cattggtgat gcagacctgc cgggaagcca ggtactccac aatctgaaag cggatcgggt
     6361 tgccgagggc tttcaacatg cgtgccagac gcttttgttc agcgttcaac gggctattca
     6421 tatcgtaatt ttacgatata acgccagttt gtcaagggtt ttgaaaatcc tcgtcagtgc
     6481 agtgaaaatg aatcacctgg gattcggtgt tgaacaggtt gcttccttgc agacaatata
     6541 taatgatctg gttcaggtgg gaagaaatct ggcggcaacc tgtcaaccgg tgctgaggag
     6601 gaccaatagc gcgataatta caaatgcatt cttcgatcaa aaacaaaaat agcgtagcgg
     6661 aaggattgat gagtagagac atgactgacc tgccggttat ccgccctgcc acaatcgaag
     6721 ataccctgga catggcccag gtgcatgttg acacctggcg cacgtcttat gtcggcattg
     6781 tgccagcgga gcatctggct aacttgagct acgagcgcag tcaggctggc tggaacgagc
     6841 atatcctcaa tcaacacggg agggagtata tttttgtagc tgaggagcgc cccggaaaaa
     6901 taattggcat tacctcaggt gggccgctcc gcgaggctat ggccgggttt gacggcgagc
     6961 tgtacgtgct ttatgtgttg aaatctttcc aggggatggg gtatggaaag cggctggtaa
     7021 catgtgttgc acaggccctg ataagcgctg gttatcggtc aatgattatc tgggtcttaa
     7081 aagataaccc tgcctgtcgt ttctacgagc ggcttggtgg acagcctgtt gtggaaaaga
     7141 tggttgaaat tgggggaaag ctgctgatcg atttggccta tgcctggtcc gatatccgtg
     7201 ggttcagtga gatagcttac tgaaaaatga tctgcctgga ttaaaagcca aacgcccttt
     7261 ccgtgaggat tgggcgtttt aaactaaaaa agtctcttgg caatgaccta ctctcccagg
     7321 aggttgccct ccaagt
//
LOCUS       DAOPVS010000096         7228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_10312_length_7228_cov_46.294437, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000096 DAOPVS010000000
VERSION     DAOPVS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10312_length_7228_cov_46.294437"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1123)
                     /locus_tag="PKL11_08040"
     CDS             complement(38..1123)
                     /locus_tag="PKL11_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13593.1"
                     /translation="MPQIKIATFNTEWMILLFGGQWNKWQSPNMPKKFAGGRVGYNPN
                     APAITDTTALARRLAYVIREMNAQIIGLQEAPPLKAQLEVFVKRFLDDEYVVYHSNSD
                     WQSISSLVHRSVADKVGAWTPALPGMGDMWKKIPYYPWGAIAADQRDLHTMKRKPLML
                     TFTPGFGKSLHLMVVHTKSKISKLTAAKWTRRDPKAVAEALDARMTLSAEVMRIRLFL
                     DKLLDSSAAPLPVMVMGDMNDGPYAETLENEFMIHNILDELCGTMLYPDYHFRHAMQP
                     ATLRTASSTRFEDALNQGNIIEELIDHMVVSPSIWQGTGHFRIVPNSCQVESAIWQAA
                     CADQGPDDKREYRPSDHKPVSVTVEWD"
     gene            complement(1110..2129)
                     /gene="add"
                     /locus_tag="PKL11_08045"
     CDS             complement(1110..2129)
                     /gene="add"
                     /locus_tag="PKL11_08045"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNZ13594.1"
                     /translation="MPIDPHYPLVELHRHLDGAVRLETILDLGLKYNLPLPARDLEGL
                     RPYVQVTGQKPGVLAFLEKFEWFTKILVNYDAVRRVAWENVADAAHEGIDHIELRFSP
                     WFMAEANQLDPAGVVEAVIDGVTAARRDFNISVGLIGILSRTYGPQIASQELNALLQF
                     KTRLVGLDLAGDEAHWPGDLFVEHIHQARSAGWQITIHAGESAGAPSIWQAVHHLGAS
                     RLGHAVRAIDDPVLMDELLRSGIGIESNLTSNVQTSTVADYASHPLRAFLERGLLASL
                     NTDDPGISGIDLHYEYDVAAPAAGLSPAQISQAQRNALQTAFLSPDEKQRLLDQKGSV
                     SHASN"
     gene            complement(2163..3245)
                     /locus_tag="PKL11_08050"
     CDS             complement(2163..3245)
                     /locus_tag="PKL11_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13595.1"
                     /translation="MQTHPILYKKLEPVQIAYINAHIQSRDEIPGLLNILRSACQNVI
                     SGAPLVIIHGGAAKTGLIIEAAFPVTQPVETNNIHTRLLEAAPILTMIHTGSHQTIRE
                     TVLKIYAYLDTHAFTTSLFRREIYNVLDESNPDHNETEVQVVLHEWERLLAEGAERVL
                     GSEARQMIMSGVDTINPSSSFTEYVDWIQGAIERLDALPIDDEQKCQVVSHCAHVFPQ
                     ERIDHLREIYEHGEFDDVLREMYLDEFWYEKPIRRGNVIYMRKNPYNPEGYENATTPA
                     ERRKAYCHCSFVHPYLEEIPARLSPTFCFCGAGWYRRLWEGILGQPVKIEHVETLLKG
                     NDQCTLTITLPLSLSGECTPDMFSQQ"
     gene            complement(3340..4287)
                     /locus_tag="PKL11_08055"
     CDS             complement(3340..4287)
                     /locus_tag="PKL11_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNZ13596.1"
                     /translation="MTSTGDPESVQQIIARIPAWRDIQNLQVERIAGLTNANYRITAA
                     GEQFVLRISGPNTQHLGINRQNELAALQSAAAANLSPEVVAFLLPEGHLVTRWVEGRH
                     WNPDEYRTPENVRLLTETVKRIHALPANGAAFSPFQRVNSFWHTAQKYNVPPPSGLDA
                     FFETMHAIENDQQMDSSDWRHFCHNDLVCVNYLYIESAHTIKVLDWEFSGLGDIYYDL
                     ATVVYTHDSEGPIPHDLEEVMLGSYFGTPTDFQRQRLLGMKFMLMLFTGFWGLAQHGM
                     QQVGLVPTVLDFDYLEFAEYMFAHDIPELQAQYHAMAGA"
     gene            complement(4298..5014)
                     /locus_tag="PKL11_08060"
     CDS             complement(4298..5014)
                     /locus_tag="PKL11_08060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13597.1"
                     /translation="MPDPKEILGSISGGRMLDVATGSGGFIHFLLAGLRDYTEIIGVD
                     ASPRAADAFAAAFKDRPAIHFIQMDAHNLNFAPASFDTVCISNSLHHFETPVRILQQM
                     NRVLRPGGRFILAEMYRDGQTEPQLTHVQLHHWWAAVDQAVGDHTHFETYSRQKLIDL
                     LATQKLEDVSLFDFSDTTEDPKSPEIAAELEAVIDRYIQRASGHPDLQSRGEVLRQRI
                     HQIGFHSATTLVAVGRKPVM"
     gene            complement(5020..5784)
                     /locus_tag="PKL11_08065"
     CDS             complement(5020..5784)
                     /locus_tag="PKL11_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ13598.1"
                     /translation="MSHHLNGTVALVTGASSGIGEATARQLAAEGAAVALVARRKDRL
                     EKLVAEINALGGTAIVIAADISQQKAATAAVEQTIQQLGRLDTLVNNAGLMLLGNIEN
                     APVEEWERMIGINLNALLYVSHAALPHLLKAAADSPRQVADIVNISSVAGRRARIGAG
                     VYNATKFGVVAFTESLRQEVTGRHVRVSVVEPGVVKTELDSHMNPETRAQTMKPFADI
                     TPLESEDIAEIILFIVTRAWRSAINEVLVRPTEQAF"
     gene            complement(5865..>7228)
                     /locus_tag="PKL11_08070"
     CDS             complement(5865..>7228)
                     /locus_tag="PKL11_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13599.1"
                     /translation="SAKRCNMAPALNPSKHPSGMIGLTIVLAGQAISILASSMTGFAL
                     SIWVFQQTSSATSLGIMQTAFTLPYLLIIPLAGVMVDRYNRKMMMMVSDLAAGLGTIA
                     ILILLTTDSLQVWHFYVVNALIGLGNAFQWPAYSAAITTMVPKEQYGRANGLMSLVQA
                     GPAVVAPLLAGALLPVIKLNGILLIDIATFLLAIGALMLVHVPPPTRTVDGQAGKGGL
                     WHEATFGFKYIFARPSLLGYVILLFFANLFLGFPNSVHMPMVLLRTDNNSLILGAAQT
                     AGAISWTVGSLLMSAWGGPRRRIHGALLGWIFYCLFGNIIFGLGRSLGVWVPAILVAG
                     IGSNIGVATSQAILQAKVAPDVQGRVFSARRMLTWFPDTFTPILGGLLADYVMEPAMQ
                     SPGWLAGLFGWMVGTDPGSGMALIMIIFGFLTIFTMLSGYLNPNIRNMEDLLPDHDQL
                     PKA"
BASE COUNT         1749 a         1840 c         2068 g         1571 t
ORIGIN      
        1 tgagagtttt ttcattaaca gcaaggggag gctagctcta atcccattca accgtgactg
       61 aaaccggttt gtggtcgctg gggcgatatt cgcgcttgtc gtctgggccc tggtctgcgc
      121 aggcagcctg ccagatggcg gattcaacct ggcaggaatt gggcacaatg cgaaaatgac
      181 cggtcccctg ccagatagac ggggagacaa ccatgtgatc aatcaattct tcaataatgt
      241 tgccctgatt aagcgcatct tcaaaccggg tggacgaggc agtgcgcagg gtggcaggct
      301 gcatggcatg gcggaagtga taatcggggt agagcatggt gccgcagagc tcgtcgagga
      361 tgttgtggat cataaattcg ttttcaagcg tttcggcata ggggccgtca ttcatgtcgc
      421 ccatcaccat tactggaagc ggggcggcag aactgtcgag cagtttatcg agaaagaggc
      481 ggatgcgcat gacctcggcc gaaagggtca tgcgggcatc cagggcttcg gcaacagctt
      541 tggggtcacg cctggtccat ttggcggcag tcagttttga gatcttggat ttcgtatgca
      601 ccaccatcag gtgcaaggat ttgccaaagc cgggggtaaa ggtgagcatg agcggcttgc
      661 gtttcatcgt gtgcaggtcg cgctggtcgg cggcgatggc gccccagggg taatagggga
      721 ttttcttcca catgtcgccc atgccgggga gcgcaggcgt ccaggcgccg actttatcgg
      781 caacggagcg gtggaccagg gaactgatgg actgccagtc tgagttggaa tgatagacca
      841 catattcatc atccaggaat cgtttgacga agacttccaa ctgggctttg agcggcgggg
      901 cctcttgcaa accaatgatc tgggcattca tctcgcgaat gacataggcc aggcggcgcg
      961 ccagagcggt tgtatcggtg atggcagggg cattggggtt gtagcccacc cgcccgccgg
     1021 caaatttctt gggcatgttg gggctctgcc atttattcca ctgaccgcca aacagcagaa
     1081 tcatccactc ggtattgaag gtggcaatct taatttgagg catgggaaac cgatcctttc
     1141 tgatccagca gacgctgttt ttcatctggt gagagaaatg cagtttgcaa ggcattgcgc
     1201 tgggcctggc tgatttgggc cgggctcaaa ccagcagccg gggcagcaac atcatattca
     1261 tagtgtagat caatcccgct gattccagga tcatcggtgt tcaaactggc aaggagaccg
     1321 cgttccagaa aggcacgcag cgggtgtgag gcataatcgg ccacggtgga ggtctgaacg
     1381 ttgctggtca ggttggattc gataccgatg ccagagcgca gcaattcatc catcaagacc
     1441 gggtcatcga tggcgcgaac cgcatgacct aagcggctag cgcccaggtg atgaactgcc
     1501 tgccagatgc tcggcgcacc ggcagattca ccggcatgga ttgttatctg ccaacctgct
     1561 gaacgtgcct gatgaatgtg ctctacaaat aaatcgccgg gccagtgggc ttcatcgcca
     1621 gccagatcaa ggccaaccag ccgggtttta aattgcaaca aggcattcaa ttcctgcgag
     1681 gcaatctgag ggccgtaggt gcggctgaga atgccaatga ggccgacgct gatgttaaaa
     1741 tcacgccggg ctgcggtaac gccatcaatc acagcttcga ccacaccggc cgggtccagt
     1801 tgattggctt cggccatgaa ccagggacta aagcgcagtt caatgtgatc gataccttca
     1861 tgggcggcat ctgccacatt ctcccaggct acccgccgga ccgcatcata attcaccagg
     1921 attttggtga accactcaaa tttttccaga aaagccagga cgcctggttt ttgcccggtg
     1981 acctggacgt aggggcgcaa tccttccaga tcgcgagcgg gaaggggcag attgtatttc
     2041 aagcctaaat cgaggattgt ctcaagccgc actgcaccat ccagatggcg gtgtaattca
     2101 acgaggggat aatgcgggtc aatgggcata aagtctcctg ctggctcaga gattacaggg
     2161 gtttattgct gggagaacat atcaggcgtg cattcgccag acaaggacag cggtaatgta
     2221 atggtcagtg tacattgatc gttgcctttt agaagagttt cgacatgctc gatcttgact
     2281 ggctgaccaa ggataccctc ccataatcgg cggtaccagc cggctccaca gaagcagaag
     2341 gttggggaga ggcgggctgg aatttcctcg agatagggat gcacaaacga acagtggcaa
     2401 taggctttgc ggcgttcagc cggagtggta gcgttttcgt agccttccgg gttgtaggga
     2461 ttttttcgca tgtaaatgac gttgccgcgg cggatgggct tttcgtacca aaattcatcc
     2521 agatacattt ctcgcagaac atcatcaaac tcgccgtgtt cgtaaatctc acgcagatgg
     2581 tcaattcgtt cctgggggaa gacatgcgcg caatgagata ccacctggca tttctgctca
     2641 tcatctatgg gcagggcatc aaggcgctct atggcgccct ggatccaatc tacatattca
     2701 gtaaaagaag aggatggatt aattgtgtcc accccggaca tgatcatttg ccgcgcttct
     2761 gatcccagga ctctttcggc gccttcagcc agcaagcgct cccattcatg aaggacaacc
     2821 tgcacttcgg tttcgttgtg atccgggttg gactcgtcca gaacattgta gatttctcga
     2881 cggaacaggg aggttgtgaa ggcgtgcgtg tcgagatacg cataaatctt cagcacggtt
     2941 tcccggatgg tctggtgtga gccggtatgt atcatcgtca ggatgggggc ggcttccaac
     3001 aaacgcgtat gaatgttgtt ggtttccacg ggctgtgtca cgggaaatgc cgcttcgatg
     3061 attaagccgg ttttggctgc gccaccgtga ataatcacca agggggcgcc agaaattacg
     3121 ttttgacagg ctgatcgaag gatgttcagt aacccgggaa tctcatcacg ggattggatg
     3181 tgggcattga tgtatgcaat ctgaacaggt tcgagttttt tgtacagaat ggggtgtgtt
     3241 tgcatctgca ttttctccca gaaataattg ccaccgttct agaatagcag aaaacgggcg
     3301 ataaaacatc ccccaatttt ggctaaaaat tgatgacgat tatgcgccag ccatcgcatg
     3361 atactgtgcc tgcaattcag gaatatcatg agcaaacatg tattcggcaa actccagata
     3421 gtcaaagtct aaaactgtgg gtaccaagcc gacctgctgc atcccatgct gggccaggcc
     3481 ccagaaacct gtgaagagca tcaacatgaa cttcatgccc aggaggcgtt ggcgttgaaa
     3541 atcagttgga gtgccaaaat aagaaccgag catcacttct tccagatcat gcggaatggg
     3601 gccttcgcta tcgtgagtat agacaacagt ggcaaggtca tagtagatat cccccaggcc
     3661 cgaaaattcc caatccaaaa ctttgatggt gtgcgctgat tcgatataca ggtaattgac
     3721 gcaaaccaga tcattatgac aaaaatgccg ccaatccgaa gaatccattt gttgatcgtt
     3781 ttcaatcgcg tgcattgttt caaaaaatgc atccagaccg gaaggcggcg gcacgttgta
     3841 tttctgagcg gtgtgccaga aagaatttac ccgttgaaag ggtgaaaaag cagccccatt
     3901 ggcgggcaaa gcatgaatcc gcttaaccgt ctcggtcaac aggcgaacat tttctggggt
     3961 ccggtactca tcaggattcc aatggcgtcc ctccacccag cgggtgacca gatgaccttc
     4021 aggcagcaga aaagccacca cttccgggct aaggttagca gccgctgcgg actgcaaagc
     4081 agccagttca ttctggcggt taatccccag atgttgggtg ttggggccgc tgatacgcaa
     4141 tacgaactgc tctccggcgg ctgtgatgcg gtaattggca tttgtcagtc cggcaatacg
     4201 ttccacctgt aaattctgaa tatctcgcca ggctggaatc cgggcaatga tctgttgaac
     4261 tgattcggga tcgccggtgg atgtcattga aagatcctta cataaccggt ttacggccca
     4321 ccgccaccaa agtggtggcg ctgtgaaagc caatctggtg aatacgctga cgtaacacct
     4381 caccgcggga ctgcaggtcg gggtgcccgg aagcgcgctg gatgtagcga tcgatcacgg
     4441 cttcaagctc agccgcgatt tcaggagatt tggggtcttc ggtggtatcc gaaaagtcaa
     4501 acagggagac atcttccagt ttttgggtgg cgagcagatc aatcagtttc tggcgggagt
     4561 aggtttcaaa gtgggtgtgg tcgcccacgg cttgatctac agcagcccac cagtggtgca
     4621 gctgaacatg ggtgagttgg ggttcggttt gcccatcgcg atacatttca gccaggataa
     4681 aacgcccgcc agggcgcaaa acccgattca tctgctgcaa gatgcgtacg ggggtttcaa
     4741 agtggtggag cgagttggag atgcagacag tatcaaacga ggcgggggca aaattcaggt
     4801 tatgggcatc catctggatg aaatggatgg cgggacggtc tttgaaggct gcggcaaagg
     4861 catcggcggc gcgcggactg gcatctacac ctattatttc ggtgtagtcg cgcaaacccg
     4921 ctaaaagaaa atggataaag ccgccactgc ctgtggctac atccagcata cgcccgccgg
     4981 atatactacc gagaatttct ttggggtctg gcatagatct tagaaagcct gctcggtagg
     5041 acgcaccagg acttcgttga tggccgaacg ccaggcacgg gtgacgatga agaggatgat
     5101 ctcggcgatg tcttctgact cgagtggggt gatatcggca aagggcttca tggtctgggc
     5161 gcgggtctca ggattcatgt ggctgtccag ttcagtcttg accacaccgg gctccaccac
     5221 cgagacgcgg acatgccgcc cagtgacttc ctgacgcaag gattcggtga aggcgaccac
     5281 accgaatttg gtggcgttat aaaccccggc accaatgcgg gcccggcgcc cggctaccga
     5341 actgatattg acaatatcag caacctggcg gggtgaatcg gcagcagcct tgagcaggtg
     5401 cggcagggcc gcgtgtgaca catagagcag ggcattcagg ttgatgccaa tcatgcgctc
     5461 ccattcttcc accggggcat tttcaatatt gcccagcagc atgaggccag cattgttgac
     5521 cagagtatcc aaccgcccca actgctgtat ggtttgttcc acggcggcgg tggcggcctt
     5581 ctgctgggaa atatcggcgg caatgacgat ggcggtgcca cccagggcgt taatttcagc
     5641 taccagcttt tccaggcggt ctttacggcg ggcaaccagg gcgacggcgg caccttctgc
     5701 agccaattga cgcgcggtgg cttcgccaat gccgctgctg gcacctgtga caagggcaac
     5761 ggttccattg aggtgatgtg acatattttt ccttccttga atcaggggca gattcagtac
     5821 agcataaccc aatctatcta aaataggtgt ttcaaattat aagcctatgc tttgggtaat
     5881 tgatcgtggt cgggcagcag gtcttccata ttacggatgt tgggattcag ataccccgaa
     5941 agcatggtga aaatcgtcaa aaagccaaat atgatcatta tcagcgccat gccggaaccc
     6001 gggtccgtgc ccaccatcca gccaaacagg cccgccagcc agcccgggga ctgcatggca
     6061 ggctccatca cataatctgc cagcaggccg cccagtatcg gggtaaacgt gtccgggaac
     6121 caggtgagca tgcgacgggc cgaaaagacg cggccctgca cgtctggagc aaccttggct
     6181 tgaaggatgg cctggctggt ggcaacgcca atgtttgaac caatcccggc aacgaggatg
     6241 gcgggaaccc agacccccag gctgcgcccc aaaccgaaga tgatattgcc aaacaggcag
     6301 taaaaaatcc aacccagcaa agcgccatga atccggcgcc ggggaccacc ccacgcactc
     6361 atcaacagag agccgaccgt ccaggaaatc gcccccgcag tttgagcggc acccagaatc
     6421 aggctgttgt tgtctgtgcg caacagaacc attggcatat gaacgctgtt tggaaatccg
     6481 agaaaaaggt tggcaaaaaa cagcaggatg acgtaaccca acaaactggg ccgggcaaag
     6541 atatatttga agccaaaagt ggcctcatgc cagagaccgc cttttccggc ctgaccgtca
     6601 acagtacggg ttggcggcgg gacatgcacc agcatcaagg caccaattgc cagtaaaaac
     6661 gtagcaatat caatcagcag gatgccattc agtttaatga caggcagtaa ggcgccagcc
     6721 aggagcggag ccactactgc cgggcccgcc tgcaccaaag acatcaggcc attggcccgc
     6781 ccatactgct ctttgggaac catggtagtg atagccgccg aatatgccgg ccactgaaag
     6841 gcgtttccga gcccaatcag ggcattgacc acataaaaat gccagacctg taagctgtct
     6901 gtggtgagca gaatcaaaat tgcgatggtt cccaggccag cggccagatc actgaccatc
     6961 atcatcatct tgcggttgta gcggtcgacc atcaccccgg ccagcgggat aatcaacaaa
     7021 tatggcagcg tgaaggcggt ttgcatgata cccagcgaag tggcgctgct ggtttgctgg
     7081 aatacccaga tagacaaagc aaaacccgtc atgctggaag ccaggattga gatggcctgc
     7141 cccgctaaaa cgatcgtcag gccaatcatc cctgatggat gttttgaggg attaagggca
     7201 ggcgccatgt tacatcgctt tgcggaac
//
LOCUS       DAOPVS010000097         7212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_10349_length_7212_cov_29.241721, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000097 DAOPVS010000000
VERSION     DAOPVS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10349_length_7212_cov_29.241721"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKL11_08075"
     CDS             <1..409
                     /locus_tag="PKL11_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13600.1"
                     /translation="QGNRRGSRTVMLWSTFGMAFYPILTALTHKPELIVVFAATAGFF
                     QGGIDLVFFDELMRTVPEKYSATFVSFFQSAQYISSIVAPLLGTFLAEAIGIGPALII
                     SGIIRLLGFALFLFDRSGKKKLPVSPEAEGAVV"
     gene            406..1578
                     /locus_tag="PKL11_08080"
     CDS             406..1578
                     /locus_tag="PKL11_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ13601.1"
                     /translation="MSTDAGKTIFCTQCGQSLPVGAMICPKCGSVVSQPTTPQVESID
                     PASVKVAAQEPPKPALCQKCGRELEPGWAACPSCGDLVSPEEIQTKPGTVTPPPTDSK
                     PKRNGMGWLLGLGILALVLGCGLIVALGFLFNTTQNLLMVEGELDQVAQTATAAQAGL
                     QITLESQLATQNAAEAQLLDQQSALENQALAFSATSTAQVAADQEALRLQQEAFQATL
                     SAVMTEAGQVIYGPADGELLHKVDGFVETTWADVNVANLIIEARFYNPYGTEEGPWDY
                     GFMFRHTDTNEQYRLVISSDGQWHFIIARGDDRPELAVGPIANLDLTAGGSNLVNLWV
                     KDGTANLYVNNQWITALDVSEKIVAGDAAVCTGFYTGYEIEGKITRYEGFKVSELP"
     gene            complement(1545..2714)
                     /locus_tag="PKL11_08085"
     CDS             complement(1545..2714)
                     /locus_tag="PKL11_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ13602.1"
                     /translation="MTIIRTLVLSGGGGRGAFHAGVYKYLCESSKPGVDDEHQGSWTP
                     DIIVGTSIGAVNGAAITQGYSGEELVELWRGLREQDIQGLPPTMSWLSRSVANRVLRG
                     MIGVSLPRVPKDISTSPPPEISPALLPGMGRFSRWLLGRWGSLLDTGPLRRTLVERMK
                     INEQSLAQSQRTLLINATNVSTGQRAVFSNRPIYKRGTAQPRSDVTPGITIQRILASC
                     SIPLVYPWTVDDETRAIYWDGALVSNTPMGAALDAAANRPDEDVMEVVVVMMTPWRTQ
                     EDLGTAKAAIMPKDFGEAITYALDWVLLASFRERIDLIEAFNRLGKIGRELNDPLLSK
                     YREVRVTIVAPEDFFPAARILDYDEHNKSLIASGYQAAEKAFCRAYGSSLTLNPS"
     gene            complement(2740..3462)
                     /locus_tag="PKL11_08090"
     CDS             complement(2740..3462)
                     /locus_tag="PKL11_08090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ13603.1"
                     /translation="MQFYFIRHAQSSNNALYDSTGSWDGRSADPELTEMGWQQARALA
                     DYLQRSPADPQAAGRDAANHGGFGLTHLYCSPMLRAVDTGSVVAAALDLPLTVWPDWH
                     EGGGIYLNDSETDEPYGLPGATRAQLQARYPRIVLPDSITETGWWNRSMEAKEERPVR
                     ARRVIDDLLARHGGTEDRVAVISHGGFYNHIIWAAARAERPEKIWMVLNNTAITRIDF
                     NEDWTDMVYLNRLDHLSPGMVT"
     gene            3555..4577
                     /locus_tag="PKL11_08095"
     CDS             3555..4577
                     /locus_tag="PKL11_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HNZ13604.1"
                     /translation="MTILPTGKLPPKLLGQLLNNAPVSHPRVRLGPGVGLDCGVIETN
                     GMVQVFKTEPITFATDQIGWYAVQVAVNDIVTTGATPRWIMLSLLLPEGKTTPALVET
                     IGEQIFTACRLHNLTVLGGHTEITTGLDRPILVATVVGETTPERLITPQGAQVGNKIL
                     LTKSAGIEATAILAREMAERLQDALLPEEIEAAQNYLYEPGISVWKDAQIAQQAGRVT
                     AMHDPTEGGIVTALWELAEASDKTLQVKPAAIPISPITEKLCNALKIDPLAAISSGAL
                     LMTAAAEDSPAICRALAEAGIACAVIGDVSEGPADVQINTRCGPQRLYQVERDEVARL
                     FETPMP"
     gene            4727..5431
                     /locus_tag="PKL11_08100"
     CDS             4727..5431
                     /locus_tag="PKL11_08100"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNZ13605.1"
                     /translation="MKRSTLIIVIGVIAALLLVTAQAFAAAPQADLTGKPTPKPHDGQ
                     IDDDKGKPDKPFKLNQGKKEVYHGVILSVDAASLTVQMEDGTTATVALTAETRLHVPT
                     LGLTATAADLLPEMRVMVKARPDEAGILTALSVNVIMGDGEGDDDMEEPEPGESAGIV
                     TEYIPGTSITIQMADGAVISYGLSPNVKIVPHRRADLLDVGASVTVVLRDDMTGAMLT
                     AKGIVVHPPAGDEDGD"
     gene            5491..5562
                     /locus_tag="PKL11_08105"
     tRNA            5491..5562
                     /locus_tag="PKL11_08105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5524..5526,aa:Arg,seq:ccg)
     gene            complement(5637..6965)
                     /locus_tag="PKL11_08110"
     CDS             complement(5637..6965)
                     /locus_tag="PKL11_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNZ13606.1"
                     /translation="MNPVTPPLVSGGLPVLGHALEMMRSRENLFRRGFVEHGNIFTIK
                     LGPQYAAVVSGAEYNKIVYTQTDRSLNMQDAYTFLKASIGEVLFTANKETYYNQRPVL
                     QEVFRRERMTQYVQAMNIEVQRWLDGLGVSGEMDLTAEMLRLTQFVAGRALIGPDYER
                     ELGPGFWAMYGDISRSLDPLLPPHWPLPKFKRRDRAKAAIRQVFQGVISARRQHPERY
                     DDLITTLLQTPLKDGRFMDDPTIVSIFTGLLFAGHETTAGQAAWLVTLLLQHPETLSR
                     AQAEIGAVLTIGESLDQEKINQLEYIAWAIDETTRIRPSADLQMRTVEEPLSIGSYQL
                     PRGWRVIVNAANSHFMPEVFQDPQRFDPLRWSPERGEGKNPFSIVGFGGGMHKCTGMN
                     FAKNEMTLIAALLFQQFELELRSPEVHVIYGNGANHPSQVRVAYRRKAAL"
BASE COUNT         1634 a         1836 c         2187 g         1555 t
ORIGIN      
        1 tcagggcaac cggcgcggtt cacgcaccgt gatgctgtgg tctacttttg ggatggcgtt
       61 ttatccgatc ttgactgcat tgacgcataa gccagaattg atcgttgttt ttgcagccac
      121 ggcaggtttt ttccagggtg ggattgatct ggtgttcttt gatgaattga tgcgcaccgt
      181 gcctgagaaa tacagcgcga cgtttgtatc attctttcag agtgcgcaat acatttcatc
      241 aattgtggcg cccctgcttg gcacgtttct ggctgaagcc attggaattg gaccggcatt
      301 gatcatctct ggcatcatcc ggctgctggg ttttgctcta ttcctctttg atcgatctgg
      361 aaagaagaag ctcccggtat caccggaggc agaaggagct gtggtatgag cacagatgct
      421 ggtaaaacta ttttttgcac acagtgcggt caatctttgc cggtgggggc catgatctgc
      481 cccaaatgcg gatcagtggt aagccagccg acaacgcccc aggttgagtc aattgacccg
      541 gccagtgtga aggtagcggc acaggaaccg cccaaaccgg cactatgcca gaaatgcggg
      601 cgagaactgg agcccggctg ggcagcctgt ccaagctgtg gggatcttgt ctccccagaa
      661 gagattcaga ccaaacctgg aacagtcacg ccgccgccaa cagacagcaa acccaagcgc
      721 aatgggatgg gctggctgct gggactgggg attctggcgc tggtgttggg gtgcgggctg
      781 attgtggcgc tggggtttct cttcaacacc acccagaatt tattgatggt cgagggggaa
      841 ctggaccagg ttgctcaaac ggctaccgcg gcgcaggccg ggctgcaaat taccctggaa
      901 agccaactgg caacccagaa cgcggcggaa gcgcaactgc tggaccaaca atcggcgctg
      961 gaaaatcagg cgctggcttt cagtgcgacc agcaccgcac aggttgccgc agaccaagag
     1021 gcacttcggc tgcagcaaga ggcttttcag gctacgctga gtgcagtgat gaccgaggca
     1081 ggacaggtga tttatggacc tgccgatggg gaactgctgc acaaggtgga tggattcgtc
     1141 gaaacgacct gggcggatgt caatgtcgcc aatttgatca ttgaagcgcg tttctataat
     1201 ccttatggca ctgaagaggg cccctgggat tatggtttca tgttccggca tacagacacc
     1261 aatgaacaat accggctggt tatcagctct gatgggcagt ggcatttcat cattgcccgg
     1321 ggagatgacc gaccagagtt agcggttggg ccgattgcaa atcttgacct gacggcggga
     1381 ggatctaacc tggtgaacct gtgggtgaaa gacggaaccg cgaacctata cgtcaacaac
     1441 cagtggatta cagcgctgga cgtttctgag aaaattgtgg caggcgacgc ggcggtgtgt
     1501 actggatttt atacgggtta tgaaattgaa ggcaaaatca cccgttatga aggattcaag
     1561 gtgagtgaac tgccgtaagc gcggcaaaag gctttttcgg cggcctggta gccggatgca
     1621 atcaacgatt tgttgtgctc gtcgtaatct aaaatccggg cagcggggaa gaagtcttcg
     1681 ggagccacaa tggtcacacg cacctcgcgg tatttactga ggagcggatc gttcagctcg
     1741 cggccaattt tgcccaagcg gttgaaggct tcgatcaggt caatacgctc gcggaaagag
     1801 gccaacagga cccagtccaa cgcataagtg atggcctcgc caaaatcttt tggcatgatg
     1861 gctgccttgg ccgtgcccag atcttcctgc gtacgccagg gcgtcatcat caccacgaca
     1921 acttccatca cgtcttcatc cgggcggttg gcagcagcat cgagtgcggc gcccatgggc
     1981 gtattggata cgagggcgcc atcccaatag atggcgcggg tttcgtcgtc caccgtccag
     2041 gggtagacca gtgggatgct gcagcttgcc agaatgcgct ggatggtgat gccaggtgtg
     2101 acgtctgaac ggggctgggc agtgccgcgt ttatagatgg gacggttgct gaagacagcc
     2161 cgttggccgg tgctgacatt ggtcgcgtta atcaagagtg tgcgctggct ttgggccagg
     2221 ctctgctcat taattttcat tcgttcaacc agggtacggc gcagggggcc ggtatccagc
     2281 aggctgcccc agcgccccaa cagccagcgt gaaaagcgtc ccatgcctgg cagcagggcc
     2341 gggctaattt ctggcggcgg cgaggtggaa atatctttgg ggacgcgtgg caacgataca
     2401 ccaatcatcc cgcgcaggac gcggttggca acgctgcggc ttaaccagct catagtgggc
     2461 ggcaggccct ggatgtcctg ctcacgcagc ccgcgccaga gctcgaccag ttcttctccg
     2521 gaatagccct gggtaatggc tgcgccattg accgcgccaa tggaggtgcc cacaataata
     2581 tccggcgtcc aacttccctg gtgttcgtca tctacaccgg gtttggaaga ttcgcacaga
     2641 tatttgtaaa cgccggcatg gaaggcgccc cgaccgcccc cacccgaaag aaccaacgtc
     2701 ctaataattg tcacgggaaa cctcctgaag gttaagcgat caggtcacca tccctggaga
     2761 gaggtgatcc aaacgattga gatagaccat atcggtccag tcttcgttga aatcaatgcg
     2821 cgtgatggca gtgttgttca gaaccatcca gattttttcg gggcgctcgg cgcgggcagc
     2881 agcccagatg atatgattat aaaagccgcc atggctgatg accgccacac gatcttcggt
     2941 gccaccatgc cgggccaaca gatcatcgat cacgcggcgg gcgcgaaccg ggcgctcttc
     3001 tttggcctcc atggagcggt tccaccagcc ggtctcagtg atggaatcgg gcagaacaat
     3061 gcgcggatag cgggcctgta attgggcacg ggttgcacca ggcaacccgt agggttcatc
     3121 ggtttcagaa tcgttgaggt aaatgccgcc accttcgtgc caatcgggcc aaacggtgag
     3181 gggcagatcg agcgcggcag ctaccaccga gcccgtgtcc acggcgcgga gcatggggct
     3241 gcaataaagg tgggtaaggc caaaaccgcc gtgattagca gcatcacggc cggcagcttg
     3301 cgggtctgcc ggggaacgct gtaaataatc agccagggcc cgggcttgct gccagcccat
     3361 ttcggtcagt tcaggatcgg cactgcggcc atcccaggaa cccgttgaat cgtacagcgc
     3421 gttattagaa gattgggcgt gccggataaa gtaaaactgc attgggcgcg tctccttgtc
     3481 aatttgttca tcctatttta ttataagcta agctttttgg gttattctaa ggccacaaac
     3541 ttcaaggaat tttgatgaca attttaccga ccgggaaatt accgccaaaa ctactgggac
     3601 aactgctgaa caatgcgcca gtatcgcacc cgcgggtgcg gttagggccg ggggtggggc
     3661 tggactgcgg cgtgattgaa accaacggca tggtacaggt tttcaagacc gaacccatta
     3721 cttttgcaac cgatcaaatt ggctggtatg ctgttcaggt ggcggtgaat gatattgtga
     3781 ccactggagc aacgccgcgc tggattatgt tgagcctgct gctgccagag gggaaaacaa
     3841 ccccggcgct ggttgaaacg attggggagc aaatttttac ggcctgccgg ctccataacc
     3901 tgacggtgct gggcgggcat acggagatca ccaccggcct ggaccgcccc attctggtgg
     3961 cgacggtggt gggtgaaaca acgccggaac ggctgattac gccgcaaggc gcgcaggtgg
     4021 ggaacaagat actgctcacc aaaagcgcag ggattgaagc cacagcaatt ctggcacgcg
     4081 aaatggctga acgattacag gatgctctcc tccccgaaga gattgaagca gcccaaaatt
     4141 acctatacga acccgggatc agtgtttgga aagatgcgca gattgcccaa caagccgggc
     4201 gcgtgaccgc catgcacgat ccgacagagg ggggcattgt gacggcgctg tgggaactgg
     4261 ctgaagccag tgacaaaacc ctgcaggtca aaccggcggc aattcccatc agcccaatca
     4321 ccgaaaagct gtgcaacgct ctgaagattg atccgctggc ggcgatctca tcgggggcac
     4381 ttttaatgac tgcggcggca gaagacagcc cagcgatctg ccgggcgctg gcagaagcag
     4441 gcattgcctg tgctgtcatt ggagatgtaa gtgaaggacc agcagatgtg caaatcaaca
     4501 cacgctgcgg tccgcagcgg ctctatcaag ttgagcggga tgaggttgca aggctgttcg
     4561 aaacccctat gccttaaaca tcggctgaca tcgccaaatt aggtatatct acggattgat
     4621 ttggggacgc agaaccggta tggttaatct ggatttggtg tacggatgat gtgaaccgct
     4681 gctggtgacc gccagaagca cttaatttga tggaggttaa tgcgtgatga aacgttcaac
     4741 tttgattatt gtgattggtg tgattgcggc gctcctttta gttacagcac aggctttcgc
     4801 cgctgcccca caggctgacc tgaccggtaa gcccaccccc aagccccatg atggacaaat
     4861 tgatgatgac aaaggaaagc cggataagcc attcaagctt aatcagggca agaaagaagt
     4921 ttatcacggg gttattttga gcgtggatgc tgccagcctg accgtccaaa tggaggatgg
     4981 cacaacggct acggtggctt tgactgccga aacccggctg catgtgccca cactggggct
     5041 gaccgcgacc gcggcggatc tgctgccgga gatgcgggtg atggtcaaag ctcgccccga
     5101 tgaagccggg atactgacag cgctaagcgt gaatgtgatt atgggggatg gtgaaggcga
     5161 tgacgatatg gaagagcccg agccaggtga gagcgcaggg attgtgactg agtatatccc
     5221 cggaaccagc atcacaattc aaatggctga tggtgctgtg atttcatatg ggttgagccc
     5281 caatgtaaaa attgtgccac atcgtcgggc cgatctgttg gatgtgggtg cttcggtcac
     5341 tgtggtattg cgcgacgata tgaccggcgc aatgctgacc gccaaaggga ttgttgttca
     5401 tccgcctgcc ggggatgaag acggcgatta acgtcagcag atctccagtt tgccaagcag
     5461 cacaggtcgt ggtaaaatcg atctgttcgc gtccctgtag ctcagtggat agagcgctgg
     5521 cctccggagc cagaagcggc agttcgagtc tgcccaggga cgcctcaaag acgccgagag
     5581 gcgtcttttt ttataggatg agagttttat gttatgagca gtttattcga gtgggtttag
     5641 agggctgcct tccgacggta tgccacccgg acctgggagg gatggttggc accgttacca
     5701 tagatgacat gaacctcagg gctgcgcagt tccagttcga actgctggaa aagcagggcg
     5761 gcgatcaggg tcatttcgtt cttggcgaag ttcattccag tgcacttatg cataccacca
     5821 ccaaagccga cgattgagaa cggattcttg ccctcgccgc gctcgggcga ccagcgcagg
     5881 ggatcaaagc gctgggggtc ttgaaaaacc tctggcatga aatgcgagtt ggcggcattg
     5941 acgatcaccc gccagccgcg cggcaactgg tagctgccga tgctcagcgg ctcttccact
     6001 gtgcgcatct gcaaatcggc cgaaggacgg atgcgggtgg tttcatcaat tgcccaggcg
     6061 atatattcaa gctgattgat tttctcctga tccagcgatt cgccgattgt cagtacggca
     6121 ccaatctcgg cctgagcccg gctcaacgtt tcggggtgct gcagcagcag agtgaccagc
     6181 caggcagctt gcccggcagt ggtttcgtgc ccggcaaaga gcagcccggt gaagatgctg
     6241 acgatagtcg ggtcgtccat gaagcggcca tctttgagcg gggtttgcag cagggtggtg
     6301 atcaggtcat cgtagcgctc agggtgctgg cggcgcgcgc tgataacccc ctgaaacacc
     6361 tgacggatag cagctttggc ccggtcgcgg cgcttgaact tgggcagcgg ccagtgcggc
     6421 ggcaggagcg ggtcgagaga gcggctgatg tcgccataca tggcccagaa gccagggcct
     6481 aattcgcgct cgtaatcggg gccgatcaga gcccgcccgg cgacaaactg cgtcaggcgg
     6541 agcatttcgg ctgtcaggtc catttcgccg cttacgccca ggccatccag ccagcgctgc
     6601 acctcgatgt tcatggcctg aacatattgg gtcatgcgtt cgcgccggaa gacttcctgc
     6661 aggaccgggc gctggttgta ataggtctct ttgttagccg taaacagcac ttcaccaatt
     6721 gaggccttga gaaaggtgta agcatcctgc atgttcaggc tgcggtcggt ctgggtataa
     6781 acgattttgt tgtattcagc tccgctgact accgcggcat actgcgggcc aagtttgatg
     6841 gtaaaaatat taccgtgttc gacaaagccg cgccgaaaaa ggttttcacg gctgcgcatc
     6901 atttccaggg cgtggcccag aacgggcaga ccaccggata ccagtggggg tgtaactggg
     6961 ttcatattaa attccttttc tggtcagata cagaaggggg tgctgtaagg tggacgacta
     7021 tcttaagatt gagtatagca cggtctattc ttccctgacg aagatagcgg ctggtataag
     7081 gttaatcaaa ctgtatctat gacggactga ggtggagaga ggatgatgga tagttcttac
     7141 atattcccac cgccggagta ttagattcaa tcataagaac aaggccgaat gattgctaaa
     7201 attgtagtga ta
//
LOCUS       DAOPVS010000098         7166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_10454_length_7166_cov_63.491211, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000098 DAOPVS010000000
VERSION     DAOPVS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10454_length_7166_cov_63.491211"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..3317)
                     /locus_tag="PKL11_08115"
     CDS             complement(111..3317)
                     /locus_tag="PKL11_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ13607.1"
                     /translation="MSNREEIERAIAALEGQRSVLGDAVVDAALASLRQKLAQTSTLH
                     AVEEQRKQATILFADLSGFTALSEKLDAEELSDTINALWQRLDAVILAHGGVIDKHVG
                     DAVMAVWGATATREDDAERAVRAALAMQAELAANAGEGPTLKMRIGLNTGPVLLGVVG
                     TTGEYSALGDTVNTASRLQNAAPVGGILITHETYRLVRGIFDVQPQQPLTVKGKAEPL
                     QTYLVLAARPRPFRPGARGVEGIETRMVGRDAELATLQQAFWTAQTGSARLVILAGDA
                     GVGKSRLLWEFEKWLALMPVAVPVFQGLATPEMSQTPYGIWRDLFRRQFEILDSDPPA
                     QVRTRLAAGLAPHLDADRADLVGQLVGFDFSAAPAVQRLLNSPAFARLAQADLLHYIQ
                     SIAADQPLVILLEDLHWADPLSLEFILRVVQELPNSPILVVALTRPALFEARPAWGAG
                     DFSQRIDLSPLTPEAATTLVRDILQKVPDLPVDLQQLIVNAAEGNPFYLEELIKMFIE
                     DGVIVRGEAEWQVAPGRLKRVSVPTTLVEVLEARLDSLPTPEKAALQRAAVVGRIFWD
                     AAIAELRAEGEPLLQAPALLPDLQRRELVFRRERTAFAGAGEYTFKHAILRDVTYETV
                     LLKRRRVYHVQTARWLEAHSGERLSEYTAAIARHYQQGGENELAARWWQRAGQSAYRS
                     SGFSVAQAAFEQALTLLPAEAIQPRLDLLISLGGCLERQGSYPEARQRLTEALETARQ
                     TGDVRAQAGALNGLNLVATRQGSFDDARLLGEEALTLARQSGDAETIALALQRLGGVY
                     YYQGTYPRAVELFEAALNTFQQLGNPTGEANCLVNLGSTASIQGDHDRAIQSYNRALA
                     INRQIGERWGVSTCLSNLGTLHSYLGKYEAARRYFEEGLEIRAEIGDRWGYAACLANL
                     GWTAMLQQEYDQAVQYGVQSLEIYRALGDRRNIANSLCNLAHADAQRGEHALARRAYL
                     EALQLGWEIGALPRVLEALAGLAGLRLAAGELIPSAELLGLTTTHPATSSDVTSVAEP
                     LLARLRSALPAADLEAALARGSALSLEATVRAALQETP"
     gene            3414..4385
                     /locus_tag="PKL11_08120"
     CDS             3414..4385
                     /locus_tag="PKL11_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNZ13608.1"
                     /translation="MKVAVLANLKADAPHRPDDAPGRWDDLDEPKTIQAIMDALQQQG
                     HQAEYFPADARLPEMLGRYRPDICFNYAVGHYGESRLAQTPALLDLLGIPYTASDVQG
                     MLYSHNKQMAKKVFQAHGLPTANFIVVDDPDHFDSTRLRFPLFVKPASEGSSVGVDDH
                     AIVQNEADLTRQIQWAWSMIHAPILVEEYVSGREFTIGVLGDEALPIVEIVSPIGFYS
                     HAQKEDADSQTYRICPAPLTDEFTAELQGIARRAKQVLHLRDLCRMDLRLDAEGKPRI
                     LEVNAIPLLYPDVTQASFVCAAEVAGYSYPALINRVLQLAAARYGLA"
     assembly_gap    4735..4834
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4835..5052)
                     /locus_tag="PKL11_08125"
     CDS             complement(<4835..5052)
                     /locus_tag="PKL11_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13609.1"
                     /translation="MLNQIAFACSDITTDQYKTWLTLLANHFKFVLQLFQFFFAAHKK
                     VWHCGFSCFLQGSGILLTAASSASANIRR"
     gene            5650..>7166
                     /locus_tag="PKL11_08130"
     CDS             5650..>7166
                     /locus_tag="PKL11_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ13610.1"
                     /translation="MIQKIAAITEGHALFVSQLFALGEQRGMIRPLSGPEGLTRWHFE
                     GDTAVLLTLPQTLSKVLEERLHLMDADLRDILNIASVEGVQFTAQVVMQILQIEKFRL
                     YDRLRSLRAEHHLVVDNEDPVEALIDQYTFVHRYVREFVYAELEPGERRERHHQVAEN
                     LISLYGEAKLGKIASQLAEHYDAAREYRKAAEYALMAARAEQAHYAWAEAEQWCRFGL
                     ERVDKHLAPSAAARLHFDLLILLGSGFYQSGNFPAALQHYQEALELAEHIGVETAAKI
                     DIYQKLADASLSEARYPEALAWIEKGLKTLSGMETAAGLLRLKLDVIRARVYSHIGQD
                     QQTVVFIRQLLSDQSDLTSANPAERAEIEDQKVLAYNVLGCSCSNLGQYDSAVAAFQA
                     GIALAQRISHHPLLAMLLLNLTFTYMLMQADALAFQANEQGRSAAMETRDNESIALAQ
                     CNDGFLLGRRGDYAGAIRALKSGIAGFAAAGSEWDLPYAYADLASACLDHGDADEA"
BASE COUNT         1442 a         2037 c         2089 g         1498 t
ORIGIN      
        1 gcctgcgccg gggctgccag cgcgctaaac agggacaaga ttaaaatgaa tgataaccag
       61 cgaactgtcc ttaacatggg aatctcctcc taaacttatg gataaaaggg tcagggcgtt
      121 tcttgcagcg ccgcccgaac agtggcttca aggcttagtg cactgccgcg ggccagggcg
      181 gcctctaaat cggcagccgg cagggcagac cgcagccgcg ccagcagcgg ctcggctacc
      241 gaggtcacat cagagctggt ggccgggtga gtggtcgtca gtcccagcag ttcggctgag
      301 gggatgagtt cgccggccgc caggcgcaat ccggccagtc cggccagggc ttccaggacg
      361 cgcggcaggg cgccgatctc ccaaccgagc tgcagagctt cgaggtaggc gcggcgcgcc
      421 agggcgtgtt caccccgctg tgcatcagca tgggccaggt tgcacagcga attggcaatg
      481 ttgcggcggt cgcccagggc gcggtaaatt tccaggctct gcaccccgta ctgcaccgcc
      541 tggtcgtact cctgctggag catggcggtc cagcccaggt tggccaggca ggcggcatag
      601 ccccagcggt caccaatctc ggcgcgaatt tccaaacctt cttcaaaata gcggcgggcg
      661 gcctcgtatt ttcccaggta tgaatgcagg gtgcccagat tgctcaggca ggtggagact
      721 ccccagcgtt cgccaatctg gcggttgatt gccagggcgc ggttgtagga ttgaattgcc
      781 cggtcgtgat cgccctgaat ggaggccgtg ctgccgagat tgaccaggca gttggcctcg
      841 ccggtgggat tgcccaactg ctgaaacgtg ttcaacgcgg cttcaaataa ttccaccgcc
      901 cgcggatagg tgccctgata atagtagact ccacccaggc gctgcaaagc cagggcaatg
      961 gtttcggcat cgcccgactg gcgcgccagt gtcagcgcct cttcgcccag caggcgggca
     1021 tcatcaaagc tgccctggcg ggtggcaacc aggttcagcc cgttgagggc acctgcctgg
     1081 gcgcgcacat cgccggtctg gcgggcggtc tcaagcgcct cggtcaatcg ctggcgggct
     1141 tcggggtagc tcccctgccg ctccaggcag ccccccaggc tgatcaacag gtcgagccgc
     1201 ggctggatgg cttcggcggg cagcagcgtc agggcctgtt caaaggcggc ctgcgccacg
     1261 ctgaagccgc tgctgcggta tgcgctttgc ccggcgcgct gccaccatct ggcagccagc
     1321 tcattttcgc cgccctgttg atagtgacgg gcaatggcgg cggtatattc gctcaggcgc
     1381 tcgccgctgt gagcttccag ccagcgagcc gtctgaacgt gatagacccg gcgccgcttg
     1441 agcagcacgg tttcataggt cacatcccgc aggatggcat gtttgaaagt gtattctccg
     1501 gcgccggcaa aagctgtgcg ctcccggcgg aagaccagct cgcggcgctg caaatcgggc
     1561 agcagtgccg gtgcttgcag cagcggctcg ccttcggccc gcagttcggc aatggcggca
     1621 tcccagaaga tgcgccccac cacggcagcg cgctggaggg cagctttttc aggcgtgggc
     1681 aggctgtcca ggcgggcttc cagcacttct accagcgttg tgggcacgct gacgcgcttc
     1741 agacgccccg gcgccacctg ccattcggcc tcgccgcgca caatcacgcc atcttcgatg
     1801 aacatcttga tcagttcttc aagatagaag gggttgcctt cggcggcatt cacaatcaac
     1861 tgctgcaaat cgacgggcag gtcggggact ttttgcaaaa tatcccgcac cagggtcgtg
     1921 gcggcttcgg gcgtcagcgg ggaaagatca atgcgctgcg aaaaatctcc ggcgccccag
     1981 gcggggcggg cctcaaacag cgcagggcgg gtcagagcca ccaccagaat ggggctgttg
     2041 ggcagttcct gcaccacccg caggataaat tccagcgaga gcgggtcggc ccagtgcagg
     2101 tcttccagca gtatcaccag cggctggtcg gcggcaatgg attgaatata atgcagcaga
     2161 tcggcctggg ccagccgggc aaaagccggg ctgtttaaca ggcgctgcac agccggcgcg
     2221 gccgaaaaat caaaaccaac caactggccc accaggtcgg cgcgatccgc atccaggtgg
     2281 ggggccaggc cagccgccag ccgggtgcgc acctgcgcgg gcgggtcgct gtccaaaatt
     2341 tcaaactggc ggcggaacag gtcacgccag atgccgtaag gggtttgaga catttcgggc
     2401 gttgccagcc cctgaaagac cggcaccgcc acgggcatca gcgccagcca cttttcaaat
     2461 tcccacagca ggcgagattt gcccactccg gcatcgcctg ccaggatgac caaccgcgcg
     2521 ctgcccgtct gagccgtcca aaaggcctgc tgcagggtag ccagttcggc gtcgcgcccg
     2581 accatgcggg tttcaatgcc ttctaccccg cgcgctccgg gccggaaggg ccgcgggcgg
     2641 gctgccagca ccaggtaggt ttgcaatggc tctgccttgc ccttgaccgt cagcggctgc
     2701 tgcggctgaa catcaaaaat gccgcgcacc agccggtagg tttcgtgggt gatcaaaata
     2761 ccgcctaccg gcgcggcgtt ctgcaggcgg ctggcggtgt tcaccgtatc acccagggca
     2821 gagtattcgc cagtggtgcc caccaccccc agcagcaccg gcccggtgtt gaggccaatg
     2881 cgcatcttca acgtgggacc ttccccggca ttggcagcca gctcggcctg catggccagg
     2941 gcggcgcgca cggcgcgctc ggcatcgtct tcacgggtgg cggtggcccc ccagacagcc
     3001 atcacggcat cgcccacgtg cttgtcaatc acgccgccgt gcgccaggat gactgcatcc
     3061 agccgctgcc agagggcgtt gatggtatct gaaagctcct cggcgtccag tttttcggat
     3121 aaagccgtga agcccgacag gtcggcaaag aggatggtgg cctgcttgcg ctgctcttca
     3181 accgcgtgca gggtgctggt ttgggccagc ttctggcgca gcgatgccag tgcggcatcc
     3241 accacggcat ctcccaacac agagcgctgc ccctccagcg cagcaatggc gcgttcaatt
     3301 tcttctcgat tgctcatagc tgcctcatgt gtccagaata acccaaaatc gggggaatgt
     3361 caaacttgtg ctacaattta ttggcattcc attttccgaa aggcattctt gccatgaaag
     3421 ttgccgtatt agccaacctg aaagcagacg cgccccatcg cccggacgat gcgcccggcc
     3481 ggtgggacga tttggacgag cccaagacca tccaggccat tatggatgcc ctgcagcagc
     3541 aggggcatca ggcagaatat tttccggcag atgcccgcct gcccgagatg ctgggacgct
     3601 accgcccgga tatttgcttc aactacgctg tggggcacta cggcgagagc cgcctggcgc
     3661 agacccctgc cctgctggac ctgctgggca ttccctacac tgcttcagat gttcagggca
     3721 tgctctactc gcacaacaag cagatggcga agaaagtctt tcaggcccat ggtttgccca
     3781 cggctaattt tattgtggtg gatgaccctg accactttga cagcaccagg ctgcgctttc
     3841 cgctctttgt caaacctgcc agcgaaggct cttcggtggg ggtggatgac catgccattg
     3901 tgcagaatga agccgacctg acccgccaga tccagtgggc ctggagcatg attcacgcgc
     3961 cgattctggt ggaagaatat gtctccgggc gggagttcac cattggcgtg ctgggtgatg
     4021 aggcgctgcc gattgtggaa atcgtctcgc cgattggctt ttattcgcac gcgcagaaag
     4081 aagacgccga cagccagacc taccgcatct gcccggcgcc attgaccgat gaatttaccg
     4141 ccgagctgca gggcattgcc cgccgcgcca aacaggtgct gcacctgcgc gacctgtgcc
     4201 gcatggacct gcggctggat gctgaaggca aaccccgcat cctggaggtc aacgccatcc
     4261 cgctgcttta ccccgatgtg acccaggcct cctttgtttg cgcggccgag gttgccggct
     4321 acagctaccc cgcgctcatc aaccgggtgc tgcagctggc tgcggcgcgc tacgggctgg
     4381 cataatgatt cctggttctg agatttgtgt ggttattccc cacgcggggc gcctcctgcc
     4441 acccgaaatt caacgcaccg atctggtaga aaatgtcgaa gcattactct tcgattgcac
     4501 ggattggtat accgacctga tttatgattt tcgcgactgg ttgggtaacc ctaccattcg
     4561 atttgactac agtccgttat gcatcaacct caatcgacac cctgatcggt tggatgacgc
     4621 aaccccggtt atgttccagg ggcaacccat ttatcgacca ggccgagagc cttcaaccgc
     4681 attgcgccgc gcctggcttg atcggtttgg ggcctctttt tttcaagcca tcgcnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcgt atatttgctg aagcgctgct
     4861 ggctgctgta agtaagatgc cgctgccttg aagaaaacag gagaagccac aatgccagac
     4921 ttttttgtgg gccgcaaaga agaattgaaa caactgcagt acgaatttga aatggtttgc
     4981 cagcagcgtc agccacgttt tgtattggtc agtggtgatg tcggaacagg caaaagctat
     5041 ctggttcagc actttcttaa cctgttgtcg gggcccaggc aaaacgaacc cgtcttcatt
     5101 gggcagggca tctgctcaat ggaaactcaa gagaacggat tgtcgccgtt catgcaggtg
     5161 ctgacaagtc tgaaaacacc tgctgaagct gttttaggca acctggtcga atttacgcgc
     5221 aaggttgccc cagcctgggc agattttttg agcggcggat tgtatgctgc agcaacgctt
     5281 acggcaaccg aggctatgaa ggcgctggct caacctgtgt tttcacaaga aaatattttt
     5341 gggcaatatg ccagtatgta tctgcgggtt gctgaaaaac gccccctggt gattttttta
     5401 gatgatctac aatgggctga cgcgacatca ttgcaactgc tcttttatct ggcacgtaat
     5461 ctggctggtg ggcgcattct gtttgtgttg gccttccgcc cggtttccgg caatgatgag
     5521 gggcgaatct ttcgcgaggt gctggctaac ctgatccgcc agggtgctca aaccctggag
     5581 ttagatcaag gtctggatgt ctgggaatat gtccggcggc gctatccgct taacgacttc
     5641 caacccgctg tgatacagaa aattgcagcg attactgagg gacatgcatt atttgtgagc
     5701 cagttatttg cgctgggcga acaacgcggc atgattcgcc ctttatctgg accagaagga
     5761 ttaactcgct ggcatttcga gggtgacaca gccgtactgc tgaccctgcc tcagaccctc
     5821 agcaaagtat tggaagaacg gcttcatctg atggatgccg acttgcgcga tattttaaat
     5881 atagcgtctg ttgaaggggt gcaatttacg gctcaggtgg tcatgcagat tctgcaaatt
     5941 gaaaaattcc gcctgtatga tcgactgcgc tcactacggg ctgaacatca tcttgttgta
     6001 gataatgaag atccggtcga agcacttatt gatcaataca catttgtgca tcgctatgta
     6061 cgcgaattcg tttatgctga attggaacct ggggagcgcc gtgaacgcca tcatcaggtc
     6121 gctgaaaatc tgatttcttt atatggtgaa gcaaaactgg gaaagattgc cagccagctt
     6181 gctgaacatt atgatgcggc tcgtgagtac cgcaaagcgg cggaatatgc cttgatggct
     6241 gcccgcgccg agcaggctca ctatgcctgg gcagaggctg aacaatggtg ccgctttgga
     6301 ttagaaaggg tagataagca tcttgcccca tcagctgccg ctcggctgca ttttgacctt
     6361 ctcattttgc ttggcagcgg gttttaccag tcagggaact tcccggcagc cttacaacac
     6421 tatcaagaag cattggagct tgctgaacat ataggggtag aaaccgcagc taaaattgat
     6481 atctatcaaa aactagctga cgcctcgctg agcgaagcgc gttaccctga agcgttagcc
     6541 tggattgaaa aggggcttaa aacactttct ggcatggaaa ccgcagctgg actgctccgg
     6601 ctcaaactgg atgtcattcg cgcacgggtt tattctcaca ttgggcagga tcagcaaact
     6661 gtagtgttta ttcgccagtt actgtcagac cagtctgatc tgacaagcgc aaaccctgct
     6721 gaacgtgctg aaattgaaga tcaaaaggtg ctggcttata atgtactggg gtgctcctgt
     6781 tcaaacctgg ggcagtatga ctcggcggtt gctgcttttc aggctggaat tgcattggca
     6841 cagcgcatca gccatcaccc gctgctggct atgctgttgt taaacctgac ctttacctat
     6901 atgctgatgc aggccgatgc gttggcattt caggctaacg aacaggggcg ctcagccgcc
     6961 atggaaaccc gtgataacga aagcatcgca ctggcgcaat gcaatgatgg ctttcttctt
     7021 ggccggcgcg gcgattatgc aggagctatt cgagcattga aaagtgggat tgctggtttt
     7081 gctgcagccg ggtctgaatg ggatttgccc tatgcttatg ccgacctggc atcggcatgc
     7141 cttgaccacg gtgatgcaga tgaagc
//
LOCUS       DAOPVS010000099         7038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_10673_length_7038_cov_58.328942, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000099 DAOPVS010000000
VERSION     DAOPVS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10673_length_7038_cov_58.328942"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKL11_08135"
     CDS             complement(<1..610)
                     /locus_tag="PKL11_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ13611.1"
                     /translation="MSTSLPWYKTAVFYELYVRAFNDSNGDGWGDLQGVIEKLDYLRD
                     LGVDCIWLLPIAPSPLRDDGYDVSDYCDIHPMYGKMDDFRRLVKAVHARGLRLLVELV
                     PNHTSDQHAWFQASRDPNHPEHETYKDYYVWSETPERYQGARIIFLDTEKSNWSYDPV
                     RKAYYWHRFFSHQPDLNYDNPAVQRAMLRTIQFWIDEGVDGIR"
     gene            complement(725..1834)
                     /locus_tag="PKL11_08140"
     CDS             complement(725..1834)
                     /locus_tag="PKL11_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ13612.1"
                     /translation="MSKRAASQRHQKTISNIVINGVLILICLIWIVPTLGILVTSFRQ
                     SEKIFQSGWWTVFPHRGFVQTREINIDPTTDVNGPITIDGETHTFEEWRMGVVMPSGE
                     KYTWIGNKRALLVRVEEKRWVGFETDLTLENYKNTLTGKEIRFTDGSGNEIVRKGNNL
                     GGAFLNSLAVAVPATIIPIMIAAFAAYGFAWLNFPGRKFFFTVVVALLVVPLQIALVP
                     VLQDFTKLSLNGTFLGIWLAHTGFGLPLAIYLMFNYISTLPREILESAFIDGASNFTI
                     FTKLILPLSVPALASFAIFQFLWVWNDYLVALVLLGDQTRVVTSALASIVGEKGQDWH
                     LLTSGAFISMVLPLTVFLALQRYFVRGVTAGSVKG"
     gene            complement(1834..2955)
                     /locus_tag="PKL11_08145"
     CDS             complement(1834..2955)
                     /locus_tag="PKL11_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ13613.1"
                     /translation="MQQHAKPKITNGGPLGWLTSTLGRVLISLFVPVLTFIVLWQGFI
                     FLRETKANQLVVVAVAILWGVGGVTLLYIVANWLVEQLPEVWTRRLQPFVFVGPGLAI
                     LAWFLAIPTIRTLILSFKDANSFNWVWLENYIFAFTDRVILEAFRNNLLWMVVGTFLC
                     VSLGLIIAVLADRSRYETLYKIIIFMPMAISFVGAGVIWKFIYTYKGEGAGIAEIGLL
                     NAIVIGLGGTPQPWLQMPPWNNFFLIIIMVWLQTGYTMVILSSAIKGISADLLEAARV
                     DGANEIQIFFNIIIPSIQGTLITVTTTIVIFTLKLFDIVRVMTGGNNGTDVIANEFYL
                     QRFTYGHSGRASAIAIILLIAVIPVMIYNLRQFKERKAF"
     gene            complement(3062..4429)
                     /locus_tag="PKL11_08150"
     CDS             complement(3062..4429)
                     /locus_tag="PKL11_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ13614.1"
                     /translation="MKTNRIFAAVLSLIVLASMLLAACTPAPTAVPTEEVSTVAPLPQ
                     GQELANAYAGMYEGTVVTMQGPFTDADAVKFENSIKAFEDATGIDIQYTGSKEFESSI
                     AITVEGGNAPDIADFPQPGLLSSLVKKGYGIDLTKVIDQNWLKQNYNQSWLDMSMMDS
                     PNGEIMAGVWGRVNGKSLVWYPKAAFDAAGYKVPTTWDELIALSDQIVADGDTPWCVG
                     IESGAATGWAMTDWIEEMMLRTTTPENYDRWVAGELKFDSPEVRKAIETVGTIWFTEG
                     YVYGGRKAIATTFFGDAPKPMFDDQPKCWLHKQGNFITSFFPEGSVAGEDYSFFYLPG
                     VDAAYGSPVLVAGDIYAMFNDRPEVRAVIQYFSTGDSVKAWVEAGGAISPHKDSNLDW
                     YTNVVDRGVAEIILNASTVRFDGSDLMPGAVGAGQFWKSMTDYVTEAIDLDTALTQID
                     AAWPK"
     gene            complement(4516..5553)
                     /locus_tag="PKL11_08155"
     CDS             complement(4516..5553)
                     /locus_tag="PKL11_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ13615.1"
                     /translation="MVKRITSQDVADLAGVSRTTVSFVLNNVQRFNISTETRKKVLEA
                     AEALGYVPLASAQALASNTARAIGLVMTRSPQYISSDAFMPQIIAGMLDIVKEQKLGL
                     LIELVEPGEQRQTYLKLIRAKHIDGMILLTPRNDDEGLVELQELNIPAVVMGRLISAT
                     GLSSVDIDNRAAARTAVEHLIGLGHRRIGCILNAPVPYSSAYERLAGYQDALAQYGIT
                     PDQNWIKSADFDPMSGYTQMKLLLEEANRPTAVFIASDNVAIGAMLAMRDNGVRVPED
                     ISIVGFDDIPSARFFAPPLTTIRTPARELAQKSCFLLLDLISGRVKPPVHLTVETELI
                     IRQSSQRLFNG"
     gene            5715..6860
                     /locus_tag="PKL11_08160"
     CDS             5715..6860
                     /locus_tag="PKL11_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ13616.1"
                     /translation="MSSSLDQLHLLTDQMHLEPDLDQGAHPPVCGTSESDPGMIYRPA
                     GGPCKAVLKTLLTSACERDCFYCPFRAGRDFRRATMQPDEMASAFIHMHRAGAVDGMF
                     LSSGVAGGGIRTQDRLIDTAELLRQRQDYRGYLHLKIMPGSDRDQVLRAMQLADRVSI
                     NLEAPNDRRLAQLAPHKVFLEELLRPLRWAEQIRRTLPGQQGWRGRWPSLVTQFVVGG
                     ADETDLELLSATSYLYSQIHLARVYFSGFNPVSDTPLENRPPENPWREFRLYQASFLL
                     RDYGFDLEDLPLTPTGSLPLEADPKVLWAEINLSQAPVEINRADRPQLLRVPGIGPRS
                     VEKLLAARRLGQLRSLEALRALGVNARRAAPFILLDGHKPPLQLPLF"
BASE COUNT         1670 a         2076 c         1738 g         1554 t
ORIGIN      
        1 cgcgaattcc atccacgccc tcatcaatcc aaaactgaat ggtgcgcagc atcgcacgct
       61 gcactgcggg attatcgtag ttcaagtctg gttgatgact gaaaaagcgg tgccagtaat
      121 aggctttgcg caccgggtca tagctccagt tcgatttctc cgtatccagg aagatgatcc
      181 gggcaccctg gtaacgctct ggcgtctcac tccagacata ataatcttta taggtctcat
      241 gttccgggtg attggggtcg cgagaagcct ggaaccaggc atgttgatct gaagtgtgat
      301 tgggcaccag ctccaccagc agccgcaaac cccgggcatg caccgccttg accagccgtc
      361 gaaaatcgtc catcttgcca tacatcggat ggatgtcaca gtaatcgctc acatcatatc
      421 catcatcgcg cagcggcgat ggcgcaatgg gcagcagcca gatgcagtcc acccccaggt
      481 cacgcaaata atccagcttt tcaatcacac cctgcaaatc cccccagcca tccccgttgc
      541 tgtcgttgaa agcgcgcaca tataattcat aaaacactgc tgtcttatac caggggagag
      601 aggtgctcat aagatatcct ttccaggttt cggttgaccc gtatgcgaat taaataagcc
      661 aggccatcct atctgaaata gtcaggtctg gcttattaga gaacgtaata attgaaaggg
      721 ctgattaacc tttaaccgaa ccagccgtta cgccgcgcac aaagtagcgc tgcagcgcca
      781 ggaataccgt cagcggcaga accatgctga taaacgcacc cgaggtcagc aggtgccagt
      841 cctgcccctt ttcgcccaca atggacgcca gggcgcttgt caccacccgg gtctgatcgc
      901 ccagcagcac cagcgccacc aggtaatcat tccacaccca caggaactgg aagatggcaa
      961 acgaggccag cgctggaacc gacagcggca ggatcagctt ggtaaagatg gtaaagttgg
     1021 atgcgccatc aatgaaagcc gattccagaa tctccctcgg cagcgtgctg atgtagttga
     1081 acatcagata gattgccagc ggcagcccaa agcctgtgtg cgccagccag atgcccagga
     1141 acgtgccgtt caggctcagc ttggtaaaat cttgcaaaac cggaaccagc gcaatttgca
     1201 gcggcaccac cagcagtgcc accaccacgg tgaagaagaa ttttcgccct gggaagttca
     1261 gccacgcaaa cccataggcc gcaaatgccg caatcataat cgggataatg gtagccggaa
     1321 ctgcaaccgc cagcgagttg aggaacgccc cgcccaggtt attgcccttg cggacaattt
     1381 cgttgccgct gccatccgta aaccggattt ccttgcccgt gagggtgttt ttatagtttt
     1441 ccagcgtcag gtcggtttca aaacccaccc agcgcttctc ttccacccgc accagcagtg
     1501 cccgcttgtt gccaatccag gtatatttct caccgctggg catcaccaca cccatgcgcc
     1561 attcttcaaa agtatgggtc tcgccgtcaa tcgtaatggg gccattcaca tcagtggtcg
     1621 ggtcaatatt gatttcccgg gtttgcacaa atccgcggtg ggggaatacc gtccaccagc
     1681 cagattggaa gattttctcg ctttgacgga aggatgtgac caaaatcccc agcgtcggca
     1741 cgatccagat caggcagatc agaatcagta cgccgttaat cacgatgttg gaaatggtct
     1801 tctggtgacg ttgacttgct gctcttttgc tcatcagaat gccttccttt ccttgaactg
     1861 ccgcaggttg taaatcatca ccgggataac cgcaatcaac aggataatgg cgattgcaga
     1921 tgcccgtccg gagtgcccat acgtgaatct ctgaagataa aattcgttgg caatcacatc
     1981 ggtgccatta ttcccccctg tcatcacccg gacaatatca aatagtttta gcgtaaatat
     2041 cacaatggtc gtagtgaccg taattaacgt gccttgaatg gatggaatga tgatattgaa
     2101 gaaaatctgg atttcgttgg cgccatccac ccgggctgcc tccagcaggt cagcagaaat
     2161 gcctttaatc gccgaagaca ggatcaccat ggtataaccc gtttgcagcc agaccatgat
     2221 aataatcagg aagaagttat tccagggggg catttgcagc cagggttggg gtgtgccccc
     2281 taacccgatg acaatggcat tcagcaggcc aatttcagca atcccggcgc cttctccttt
     2341 gtaggtgtaa ataaacttcc agatcacgcc cgcacccacg aaagaaatcg ccatcggcat
     2401 gaagatgatg atcttgtaaa gcgtctcgta tcggctgcgg tcagccagca ccgcaataat
     2461 caaccccagg ctcacacaca ggaatgtgcc taccaccatc cagagcaggt tgttgcgaaa
     2521 cgcctccaga ataacccggt ctgtgaaggc aaaaatgtag ttctcaagcc agacccagtt
     2581 gaagctgttg gcatctttaa aacttaaaat caatgtgcgg atggtcggaa ttgccaggaa
     2641 ccaggccaga attgccagcc cgggtcccac aaaaacaaac ggttgcagcc gccgggtcca
     2701 cacctctggc agttgttcca ccagccagtt ggccacaatg taaaggaggg tcacaccgcc
     2761 aaccccccac aggatggcaa ctgctaccac caccagttgg ttcgccttcg tctcgcgcag
     2821 gaagatgaat ccctgccaca aaacgatgaa ggttagcacc ggcacaaaca gagagattaa
     2881 tactcttccc agggtgcttg tcaaccaacc taaagggccg ccgttggtaa tcttgggctt
     2941 agcatgttgt tgcataagtc acttcctccc atccgacaat taggaatgaa ggagcagggg
     3001 tctgatttga ttcatttttc ctgctccttc attattatat agcgtactta gttacttcag
     3061 gctatttcgg ccaggctgca tcgatctgag tcagtgcagt atccaggtca attgcctcgg
     3121 tcacatagtc cgtcatagac ttccagaact gaccagcgcc cacagcgccc ggcatcaggt
     3181 cagaaccatc gaagcgcacc gtggaggcgt tcaggatgat ttcagcaacg ccgcggtcca
     3241 caacattggt gtaccagtcc aggttggagt ctttgtgggg ggagatggcg ccgccagctt
     3301 caacccaggc tttgacagag tcaccagtcg agaagtactg gatcacagcg cggacttccg
     3361 ggcgatcgtt gaacatggca tagatgtcgc cagcaaccag cacaggggag ccgtaggccg
     3421 cgtccacacc aggcagatag aagaacgagt aatcttcacc agcaacactg ccttcgggga
     3481 agaaggaggt gatgaagttg ccctgcttgt gcagccagca cttgggctga tcatcgaaca
     3541 tgggcttggg ggcatcgccg aagaaagtcg tggcaatggc tttgcggcca ccgtacacat
     3601 aaccttcggt gaaccagatg gtgccaacgg tctcaattgc tttgcgaact tcgggtgaat
     3661 cgaatttcaa ttcgccggca acccagcggt cgtagttttc gggggtggtg gtgcgcagca
     3721 tcatctcttc gatccagtcg gtcatcgccc aaccggtcgc cgcacccgat tcaatcccaa
     3781 cgcaccaggg ggtgtcgcca tcggcaacaa tctggtccga cagggcaatc agttcgtccc
     3841 acgtggtcgg aactttgtag ccagcagcat caaaagcggc cttgggatac cacaccagcg
     3901 acttgccatt cacacggccc cacacacccg ccatgatttc gccattcggg ctgtccatca
     3961 tggacatatc gagccaactc tggttgtagt tctgcttcag ccagttctgg tcaatcacct
     4021 tggtcaggtc aatgccgtaa ccttttttca ccagcgagct gagcagaccc ggctgcggga
     4081 agtccgcaat atcgggggca ttgccgcctt caacggtgat ggcgatggaa gattcaaatt
     4141 ccttcgagcc agtgtattga atatcaatac cggtggcatc ttcaaaggct ttgatcgagt
     4201 tttcaaattt caccgcgtca gcatcggtga agggaccttg catggttaca acagtgcctt
     4261 cgtacatacc ggcgtaagca ttcgccagtt cctggccctg gggcaggggg gcaacggtag
     4321 aaacttcttc agtgggcaca gccgtcggag cgggcgtgca ggcggccagc agcatggatg
     4381 ccagaacaat caggctcaag actgctgcaa aaatacgatt ggttttcata gttctcctct
     4441 ttaaggtaag tgtgattggg ttttgaacat cagtgaatag aaatttattg gcttgtttca
     4501 ataggcctcc tttgactaac cgttgaaaag tcgttgcgat gattggcgga taatcagctc
     4561 tgtctccacc gtcaggtgaa ccggcggctt cactctacca ctgatcaagt ccagcagtaa
     4621 gaaacaggac ttttgggcca actcacgcgc tggcgttcga attgtggtta gcgggggggc
     4681 aaagaatcgt gctgaaggaa tatcatcaaa accaacaatt gagatatcct ccgggactct
     4741 taccccatta tcgcgcattg ccaacatcgc gccaatagcc acattatcgc tggcgatgaa
     4801 aactgcagtt ggccgattag cctcctccag caataatttc atttgggtat aaccgctcat
     4861 cggatcgaaa tccgcagact tgatccagtt ttgatcgggc gtaatgccgt actgagccag
     4921 cgcatcctgg taccctgcca ggcgttcata tgccgaactg tagggaactg gcgcattcag
     4981 aatgcagcca atccgccggt gtcccagccc aatcaagtgt tcaaccgctg tccgggcggc
     5041 cgcccgatta tcaatatcta ccgaactcaa cccggtggct gagattaatc ttcccatcac
     5101 cactgccgga atattgagct cttgcaactc aaccaaaccc tcatcatcgt tgcggggggt
     5161 tagtaaaatc attccgtcaa tatgtttggc gcgaatgagc ttgaggtagg tttgccgctg
     5221 ctcgccgggc tccaccagtt cgatcagcag gcccagcttc tgctccttca caatatccag
     5281 catccccgca ataatttgcg gcataaatgc gtcagaagaa atatactgtg ggctgcgcgt
     5341 catcaccagc ccaatcgcgc gagccgtatt cgatgccagc gcctgtgctg atgccagcgg
     5401 cacatagccc aaggcttcag ccgcttccaa tacctttttc cgcgtctcag ttgaaatatt
     5461 aaaccgctgg acattgttca aaacaaacga aaccgtagtc cgagagaccc cggctaaatc
     5521 tgcaacgtcc tgactggtaa tccgtttgac cacgtaaata tttcctgaga gggggattag
     5581 aaatcaacta gtaacacgcg ttattaaacc gcgttaacag tatagcacgg tttttgtaac
     5641 tgtcaatcat ccattttgaa aaaatgtgat taatttggct tgaataaaca gaaatattgt
     5701 tctataatag aggtatgagc agttcacttg atcagcttca cctcttgacc gatcagatgc
     5761 acctcgaacc cgacctggac cagggtgccc atcccccggt ttgcggcaca tctgaatccg
     5821 accccggcat gatctaccgc cctgccggcg gcccctgcaa ggctgtcctc aaaaccttgc
     5881 tcacctctgc ctgcgagcgg gactgcttct actgcccctt ccgcgccggg cgagatttcc
     5941 gccgcgccac catgcagccc gatgaaatgg cctctgcttt tatacacatg caccgtgccg
     6001 gtgcggtgga tggcatgttc ctgtcctctg gcgtggctgg cggcggcatc cgcacccagg
     6061 accgcctgat cgacaccgcc gaactgctcc gccagcgtca ggactaccgc ggctacctcc
     6121 acctcaaaat catgcccggc tcagaccgcg accaggtctt gcgcgccatg cagctcgccg
     6181 accgcgtctc cattaacctg gaagccccca atgaccgccg tttggcgcag cttgccccgc
     6241 acaaagtttt tctcgaagag ctcctgcgcc cgctgcgctg ggcagagcag attcgccgca
     6301 cgctgcccgg acagcagggc tggcgcgggc gctggccctc cctggtcacc cagtttgtgg
     6361 ttggcggtgc cgatgaaacc gacctggaac tgctctccgc cacctcttac ctgtacagcc
     6421 agattcactt ggcccgggtc tatttttccg gcttcaaccc cgtctccgac acgcccctcg
     6481 aaaaccgccc acccgaaaac ccctggcggg agtttcgcct ctatcaggcc tccttcctgt
     6541 tgcgagatta tggctttgac ctggaagacc tgccattgac ccctactggc agcctgcccc
     6601 tggaagccga ccccaaagtc ctctgggcgg agataaattt gagccaggcc ccggtggaaa
     6661 ttaaccgcgc cgaccgcccg cagctcctgc gcgtccccgg cattggcccc cgcagtgttg
     6721 aaaaactgtt ggccgcccgc cgcctggggc agctccgcag cctcgaagcc ctgcgagccc
     6781 tcggcgtcaa tgcccgccgc gccgccccct tcatcctcct ggatggtcat aagccccccc
     6841 tccagcttcc cctcttctag tcaaccgaat atccccctgc ccccacaacc ccgcaaaatt
     6901 actccaaccc gtaaccccac cccgtgtaat ccataaaacc tgcctcagca gtgaaatctg
     6961 tgtaatctgc ggttgtaatc tgcggttccg gtttgcattc cccctccaat tcgagtaaaa
     7021 tagacaaatc ttatcaag
//
LOCUS       DAOPVS010000100         6914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_10912_length_6914_cov_59.086164, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000100 DAOPVS010000000
VERSION     DAOPVS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10912_length_6914_cov_59.086164"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKL11_08165"
     CDS             <1..267
                     /locus_tag="PKL11_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13617.1"
                     /translation="QFLALSVVLGNAPTPTRASATLVPPPGETPAPVLIITPPPATEE
                     PVETAEPQETDEPDDSDAADDAVETETVEPAETEETDKTEAPGG"
     gene            289..1461
                     /locus_tag="PKL11_08170"
     CDS             289..1461
                     /locus_tag="PKL11_08170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNZ13618.1"
                     /translation="MTRKNLLLTILLALSLLSALFGGARPAAAQTAPADDADLPLCLP
                     DAYPTAPADCLALGPSPYLTELARKGIDYPFRPLPAYRPDPAMGYSDINYLKVGETAL
                     PLYASLEDAMSGSAVQSLGSGLKYLAVGGRVDNDGGAFYPTTSGLWVAARDTEAACCI
                     RSGRIWGLVFASNPASSFGWIVDQAELHAAPGYAAPKLPTSLYRETVVQIYDKVSADG
                     TDWYMLGMNTWVERRYIRQFVVNLTPPQGVENGRWIEINLYEQTLGVYENGRLVYATL
                     IASGAEPYYTRPGLFQIYEKLDKTTMSGAFEADRSDFYYLEEVPYTMYYDEARALHGA
                     YWRTLYGYPQSHGCVNLSIGDARWLYEWAQVGDWVYVWDPSGETPTDPALYTEGGA"
     gene            1619..2221
                     /locus_tag="PKL11_08175"
     CDS             1619..2221
                     /locus_tag="PKL11_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNZ13619.1"
                     /translation="MFDMVEMMIDSVRVSLTNQQRIVILRQLDVERYLPIWIGPYEAE
                     AITIALQEIEVARPQTHDLLKNTLTTLGARLLRIEVIALREDVFYAHLIVEISDRLIE
                     IDSRPSDALALAVRFHAPIYVAQEVLNTAGVTPEEDVQQTPQSVTSPAPAEEPSSDRL
                     SIFEDFLQNLDLDDLDTPHPDDEPGPDPNSDPDPDQPAGQ"
     gene            2224..3618
                     /gene="dnaB"
                     /locus_tag="PKL11_08180"
     CDS             2224..3618
                     /gene="dnaB"
                     /locus_tag="PKL11_08180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNZ13620.1"
                     /translation="MSDLPPFEDEAPQPELQAQPHSREAEEAVLGAVLINPETYYNIA
                     QFLRSDDFYIVRNRWLWEAFNRLHERRIPLDYLTVCQELEEGEHLTEIGGPAYVTALI
                     NQTPTSMHAEAYGRIVEAHSVRRRMLQAANEMARLAYDQSQTVDNILGEAEKAVFGLS
                     ERRIQHDLQPISTVLSTYYDRVDSLSQLGDDIYGVPTGLYDLDKLLGGLQKSDLLIIA
                     GRPGMGKTGFLLSIAKNAGQKHKKHVAIFSLEMSGEQLVQRLIAQETGIDTQRLRTGK
                     LEEHEWPLFTHAVEVLADTRIWLDDTPSITPLSLRTKCRRLDMEFGLDLVILDYIQLM
                     SSDTRTDNRVQEVSYISRSLKNLARELNVPVLAAAQLSRAVEQRTDKRPQLSDLRESG
                     SLEQDADIVMFINRPDALEKDSPRANMAEIIVAKHRNGPTHPGIELVFLNNLARFENA
                     ATPGQPPAGGARRR"
     gene            3630..4334
                     /locus_tag="PKL11_08185"
     CDS             3630..4334
                     /locus_tag="PKL11_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HNZ13621.1"
                     /translation="MPSFTGFPAGQTNPTPVPNAFFTDLLPEIDHLDELRITLYAFWA
                     LNQQEGNLRFLARSDFERDKRLMHSLGRTKAKAQAALTNALERAVLRGSLLQAFLRPG
                     EAAEEALYFINTVRGRAALDALKNGEWSPADQARPEITLDLERPNIFRLYEQNIGPLT
                     PLMADALREAEELYPAEWIEEAISAAVKANARSWRYAEAILKRREKGRDGTARGKTEK
                     SSRRYVEGELGEFVEH"
     gene            4255..5676
                     /locus_tag="PKL11_08190"
     CDS             4255..5676
                     /locus_tag="PKL11_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ13622.1"
                     /translation="MEPLEEKLKRVAAATSKANSANSLNTEPTRSNLPGDPNCPICHG
                     IGFVRQDLPIDHPDFGRMTVCTCRQKDAVTSAQQRLFAQSNLAALSAMTFETFKAEGR
                     RGLSDQHLLSLRTARAQAQQFAATLPGWLVIEGGYGCGKTHLAAAIANHAVSLGVPTL
                     FLTVPDLLDWMRFAYSDPGATFEQRFEEIRNVRLLVLDDLGTQNATAWAQEKLFQILD
                     YRYLHRLPLVITTNLEPGDLDGRIRSRLQDVTLVTTLKISAPDYRTGSNEAEQSLLST
                     LNLLNDRTFGNFSMREPDRLPTDVQRSLEKSFRAAQEFAERPRGWLVLMGTYGCGKTH
                     LAAAIGNYRAAGGEEPLFVVVPDLLDHLRAAFSPNSTQSYDRLFEEVRNARLLILDDL
                     STQSATPWAREKLYQIINHRYNASLPMVITTASSLDEIDPRIQSRLLDSRLCKIYAIL
                     APAYRAATAPLKRPRTTRSSKTG"
     gene            complement(5686..>6914)
                     /locus_tag="PKL11_08195"
     CDS             complement(5686..>6914)
                     /locus_tag="PKL11_08195"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNZ13623.1"
                     /translation="KNFEKGKTMPKLWKILGLLLAVTLLAGCGSLSPLPTAIPTSTLP
                     ATATPFQPAPPTATAAVPTTVPTPTLAPTPVYPGYGPNSFPAEINPLTGLAPQDPNLL
                     NRRPIIIKVQNLPRYDRPQYGLSLADLVFEYYTEEGSTRFAALYYGQDATQVMPIRSA
                     RHHDIHLIRAYKAVFLFGYADPRVYERLISAEFYQRLLLESDRAYPALTRFGPSNFLM
                     ANTAEVQAVCARNGIDNSRQNLDGMRFELAAPGGGTPATQVDVHFSAAIYNRWTYDAA
                     SGRYQRASDTQNAADAASEVYAPLTDALTGAPIAADNVVILFVPHEDREPNPEVEMPE
                     AVLLGQGPAWIARDGQIYPVTWTRPSESSILTLVDASGQPFPFKPGQTWIEVLTASSK
                     HEASGSAVRFTFWREW"
BASE COUNT         1347 a         2325 c         1928 g         1314 t
ORIGIN      
        1 cagttcctgg cgctctcggt ggtgctgggc aatgcgccca cccctacccg ggcatcggcc
       61 accctggttc cgccacccgg tgaaaccccc gccccggtgc tgattatcac gccgccgccc
      121 gcaaccgaag aacctgtcga aaccgcggag ccgcaggaaa ccgatgaacc tgatgattcc
      181 gacgcggcag atgacgctgt tgaaaccgaa accgttgaac ccgccgaaac tgaagaaaca
      241 gacaaaaccg aggcccccgg cggctaaaat gacgttataa tccaccccat gactcgaaag
      301 aacctgctgc tcaccatttt actggcgctc agcctgctga gtgctctgtt tggcggcgcc
      361 cggcctgccg cggcacaaac cgcccccgct gacgatgccg acctgccgtt gtgcctgccc
      421 gatgcctacc ccaccgcccc cgcggactgc ctggcgctgg gcccttcccc ctacctgacc
      481 gaactggcgc gtaaaggcat cgattatccc ttccgccccc tgccagctta ccgccccgac
      541 cccgccatgg gctattctga catcaattac ctcaaggttg gcgagaccgc cctgccgctg
      601 tatgcctcgc tggaagacgc catgtctggc agcgccgtcc aatcgctggg ctccgggctc
      661 aaatacctgg cggttggcgg ccgcgtggat aatgacggtg gcgcttttta ccccaccacc
      721 tcgggcttgt gggtggctgc ccgggataca gaggccgcct gctgcatccg ctccgggcgc
      781 atctgggggt tggtctttgc gtccaacccg gcctcgtcct ttggctggat tgtggatcag
      841 gctgaactgc acgccgcgcc cggctatgca gcgcccaaac tgcccacctc gctgtaccgc
      901 gagactgtgg tgcagattta cgacaaggtc agcgccgacg gcaccgactg gtacatgctg
      961 ggcatgaata cctgggttga gcggcgctac attcgtcagt ttgtggtcaa cctcacccca
     1021 ccccagggcg tggaaaatgg ccgctggatt gaaatcaacc tctatgagca gaccctgggc
     1081 gtctatgaga atgggcggct ggtctatgcc acgctgatcg ccagcggcgc cgagccctat
     1141 tacacccgcc cgggcctctt ccagatttat gagaagctgg acaagaccac catgtctggc
     1201 gcctttgaag ccgaccgctc cgatttctac tacctcgaag aagtgcccta taccatgtac
     1261 tatgatgagg cgcgggcttt gcacggcgcc tactggcgca ccctctacgg gtatccgcag
     1321 tcgcatggct gcgtcaacct ctccattggc gacgcccgct ggctctacga gtgggcgcag
     1381 gttggcgact gggtttatgt ctgggatccc tccggcgaaa cccccaccga tcccgccctc
     1441 tacaccgaag gcggtgcctg agccccggga tttaggattg agacctcatc ctgtgcatgg
     1501 gttcgcgctc tggggtctca atcataaatc aacaatctgc aatccaactg gttgcatctc
     1561 catgccgccc gtgttaaaat aactcaggga ctattattct tccagcggga ggctgttcat
     1621 gtttgatatg gtcgagatga tgatagacag cgtgcgggtc agcctgacca atcagcagcg
     1681 cattgtgatt ttgcgccagc tcgacgttga acgttacctg cccatctgga tcggtcccta
     1741 cgaggccgag gccattacca tcgccttgca ggagattgaa gtggcccgcc cgcagaccca
     1801 cgacctgctc aagaataccc tcaccaccct gggcgcccgc ctgctgcgca ttgaagtcat
     1861 cgccctgcgc gaagatgtct tctatgccca cctgattgtc gaaatctccg accgcctgat
     1921 cgagattgac tcccgcccct ccgatgccct ggcgctggca gtgcgcttcc atgcacccat
     1981 ttatgttgcg caagaagtct tgaataccgc cggcgtcacc cccgaagaag acgtccagca
     2041 gaccccgcaa agcgtcactt ccccggcccc ggctgaagag ccatcctctg accgcctgtc
     2101 catttttgaa gattttctcc agaaccttga cctggatgat ctggataccc cccacccgga
     2161 cgacgaaccc ggccccgatc ccaactctga tcctgatccc gatcagcccg caggtcaatg
     2221 agcatgtctg acctgccgcc ctttgaagac gaagccccgc agcccgaact tcaggcccag
     2281 ccccacagcc gggaggccga agaggcggtg ctgggcgccg tcctgattaa ccccgaaacc
     2341 tactacaata ttgcccagtt cctgcgctcc gatgatttct acattgtccg caaccgctgg
     2401 ctctgggagg ccttcaaccg cctgcacgag cgccgcatcc ccctcgatta cctgaccgtt
     2461 tgccaggaac tggaggaagg cgaacacctg accgaaattg gcggcccggc ctatgtcact
     2521 gcgctgatca accagacccc cacctccatg cacgccgaag cctacggccg cattgtggag
     2581 gcccactcgg tgcggcggcg gatgctgcaa gccgccaatg agatggcccg cctggcctat
     2641 gaccagagcc agactgtgga caacatcctc ggtgaggccg aaaaggcggt ctttggcctg
     2701 tcggagcggc gcatccagca cgacctgcag cccatttcca ccgtcctcag cacctattac
     2761 gaccgggtgg actcgctctc ccaactcggg gatgatatct acggcgtgcc caccggcctg
     2821 tatgacctgg ataagcttct gggtgggctg caaaaatctg acctgttgat tattgccggt
     2881 cgcccgggca tgggtaagac gggcttcctg ctttccattg ccaaaaacgc cgggcagaaa
     2941 cacaaaaaac atgtggccat cttctcgctg gaaatgtctg gcgagcagct tgtccagcgt
     3001 ctgatcgccc aggaaaccgg cattgatacc cagcgtctgc gcaccggcaa actggaagaa
     3061 catgaatggc cgctcttcac ccatgcggtg gaagtgctgg ccgatacccg catctggctg
     3121 gacgacaccc cctccatcac cccattgtcg ctgcgcacca agtgccgccg cctggatatg
     3181 gaatttggcc ttgatctggt catcctggac tatatccagt tgatgtccag cgacacccgc
     3241 accgataacc gcgtgcaaga ggtctcctac atctcccgca gcctcaaaaa cctggcgcgt
     3301 gaactcaacg ttccggtgct ggcggctgcc cagctctccc gtgcggtaga gcagcgcacc
     3361 gacaagcgcc cgcagctctc tgatctgcgt gagtcgggct cgctggagca ggacgccgac
     3421 attgtcatgt tcatcaaccg ccccgatgcc ctggaaaaag acagcccgcg ggccaatatg
     3481 gccgagatca tcgttgccaa gcaccgcaat ggccccaccc atcccggcat cgagctggtc
     3541 ttcttgaata acctggcgcg ctttgaaaac gccgccacac ccggtcagcc gcctgctggc
     3601 ggcgcccgcc gccgctaaag gaggctgcag tgccatcttt cacgggcttc ccggccggtc
     3661 agaccaaccc caccccggtg ccaaacgcct tctttacaga tctgctgcca gaaattgacc
     3721 atctggatga actgcgcatc acgctgtatg ccttctgggc cctgaaccag caggagggca
     3781 atctgcgctt tcttgcccgc agtgactttg aacgtgacaa acgcctgatg cactcactgg
     3841 gccgcaccaa agccaaagcc caggctgccc tgaccaatgc gctggagcgc gcggtgctgc
     3901 gcggctcgct gctgcaggcc tttttgcgcc ccggcgaggc tgctgaagag gcgctttact
     3961 tcattaatac agtgcgcggt cgcgccgctc tggatgccct gaagaacggc gaatggtcgc
     4021 ccgccgacca ggcccgcccc gaaatcaccc tggatctgga acgccccaac atcttccggc
     4081 tctatgaaca aaacatcggc ccgctcaccc ccctgatggc ggatgcgctg cgtgaggccg
     4141 aagaactcta ccccgccgaa tggattgaag aagccatttc ggcagcagtc aaagctaatg
     4201 cccgttcctg gcgctacgca gaggccatcc tcaaacgccg ggagaaagga cgcgatggaa
     4261 ccgctcgagg aaaaactgaa aagagtagcc gccgctacgt cgaaggcgaa ctcggcgaat
     4321 tcgttgaaca ctgagccgac ccgctccaac ctgccgggcg atcccaactg ccccatctgt
     4381 catggcatcg gctttgtgcg ccaggacctg cccattgatc atcccgattt tggccggatg
     4441 accgtctgca cctgccgcca gaaagatgcc gtcacttcag cccagcagcg cctgtttgcc
     4501 cagagcaatc tggcggcgct ctcggccatg acgtttgaga cctttaaggc cgaaggccgc
     4561 cgcggccttt cagaccagca cctgctgtcg ctgcgcactg cccgcgccca ggcgcagcag
     4621 ttcgccgcca ccctgcccgg ctggctggtc attgaaggcg gctatggctg cggcaaaacc
     4681 cacctggccg cagccatcgc caaccatgcc gtcagcctgg gcgtgcccac cctgttcctc
     4741 accgtccccg acctgctgga ctggatgcgc tttgcctaca gcgaccccgg cgccaccttt
     4801 gagcagcgct ttgaagaaat tcgcaacgtg cgactgctgg tgctggatga tctgggcacc
     4861 cagaacgcca cggcctgggc gcaggaaaaa ctcttccaga ttctagatta ccgctacctg
     4921 caccgcctgc cgctggtgat taccactaac ctggagcccg gcgacctgga cgggcgcatc
     4981 cgctcgcgcc ttcaggatgt caccctggtc accaccctca aaatcagtgc gcccgattac
     5041 cgcaccggct ccaacgaagc cgaacagtcc ctgctttcaa ccctcaacct gttgaatgac
     5101 cgcacttttg gcaatttcag catgcgcgaa ccagaccgcc tgccgaccga tgtgcagcgc
     5161 agcctggaga agagcttccg cgccgcccag gagtttgccg agcgcccgcg cggctggctg
     5221 gtgctgatgg gcacctacgg ctgcggcaag acgcacctcg cggctgccat tgggaattat
     5281 cgcgcggctg gcggggaaga gccccttttt gtggtcgtcc ccgacttgct cgatcacctg
     5341 cgggcggcct tcagccccaa cagcacccag tcttatgacc gcttgtttga agaagttcgc
     5401 aacgcccgat tgttgatttt ggatgacctg tccactcaga gcgccacacc ctgggcgcgt
     5461 gaaaagctct atcagatcat caatcaccgc tataacgcca gcctgcccat ggtcatcacc
     5521 accgccagct cgctggatga gattgacccg cgcatccaga gccgcctgct cgacagccgc
     5581 ctgtgcaaaa tttacgccat cctggcgccc gcctaccggg ccgccacggc gccgctcaag
     5641 cgcccgcgca ccacgcgcag cagcaaaacc ggctgaagca ggctgctacc actctcgcca
     5701 gaacgtgaac cgcaccgctg aaccgcttgc ctcatgttta gagctggcgg tcagcacctc
     5761 aatccaggtt tgccccggct tgaacgggaa gggctgcccg ctggcatcta ccagcgtcag
     5821 gatgctgctt tcgctggggc gcgtccaggt caccgggtaa atctgcccgt cccgggcaat
     5881 ccaggcgggg ccctgaccca gcagcaccgc ctcgggcatc tccacctcgg ggttgggctc
     5941 gcggtcttcg tgcggcacaa acaggatcac cacattatcg gcggcaatcg gggcgccagt
     6001 cagcgcatcg gtcaacggtg cgtaaacttc gctggcagca tcggcagcat tttgcgtgtc
     6061 cgaagcccgc tggtagcgtc cgctggcggc gtcatacgtc cagcgattgt aaatcgccgc
     6121 tgagaagtgc acatccacct gggtggctgg tgtgcccccg cccggagcag ccaactcaaa
     6181 gcgcatgccg tccaggttct gccgggaatt atcaatccca ttgcgggcgc aaaccgcctg
     6241 cacttcggcc gtgttggcca tcaggaaatt gctgggccca aagcgcgtca gcgccgggta
     6301 agcgcgatca ctttccagca gcaggcgttg ataaaattct gccgaaatca gccgctcata
     6361 aacacgcggg tcggcataac cgaataaaaa cacagcttta taggcccgga tcaggtggat
     6421 atcatgatgc cgcgccgagc ggatgggcat cacctgggta gcatcctgcc cgtaatacag
     6481 cgccgcaaac cgcgtggagc cttcctcggt gtaatattca aataccaggt ccgccagcga
     6541 cagtccgtac tgcggccggt cataccgcgg caggttttgc accttgatga tgatcgggcg
     6601 gcggttcagc aggttggggt cttgcggcgc cagcccggtc agcgggttga tttcggcggg
     6661 aaagctattg ggcccatagc ccggatagac tggcgtgggc gccagggtgg gcgttggcac
     6721 agtcgttggc acagccgcag tggcggttgg cggcgctggt tgaaacggcg ttgccgtcgc
     6781 cggcagggtt gaggtgggaa tcgctgttgg cagcggtgaa agtgacccgc agccagccag
     6841 cagtgtgact gccagcagca ggccaaggat tttccagagt ttgggcatgg ttttgccttt
     6901 ctcgaaattt ttat
//
LOCUS       DAOPVS010000101         6603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_11572_length_6603_cov_58.543525, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000101 DAOPVS010000000
VERSION     DAOPVS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11572_length_6603_cov_58.543525"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKL11_08200"
     CDS             <1..329
                     /locus_tag="PKL11_08200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNZ13624.1"
                     /translation="PLLPLVLLFASLNAFNEEMTYRASLLAPLENVVGYKHALGITAA
                     FFGLGHFYGVPYGLVGVAMATFLGWLLGKAMLETRGFCWSWLIHFLQDVLIFSFMAIG
                     SIVPGG"
     gene            complement(338..1252)
                     /locus_tag="PKL11_08205"
     CDS             complement(338..1252)
                     /locus_tag="PKL11_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNZ13625.1"
                     /translation="MKKGFIFIMAGLMFALAGCTPTATPPAADQPLRVVAAEAFLADI
                     TQNIAGERLTVTALIPAGVDPHTFTPTPQDLALITDSDVLILNGAGLEEALLPVLENA
                     DGERLLIEAAAGLESRPVEADAHGHTEGDPHFWLDPNLAMAYVETIRAGLTAADPAGG
                     ELYAANAAAYTAELQALDAWIREQIETIPPERRLLVTNHESFGYFADRYGLTVVGALA
                     TSVSAGAAPSAQQVAELVDQIRATGAPAIFLEQGVNPQLAEQVAAETGAQVVTGLYTH
                     SLTPPGGEAPTYIEMLRFNVTQIVTALR"
     gene            complement(1300..1722)
                     /locus_tag="PKL11_08210"
     CDS             complement(1300..1722)
                     /locus_tag="PKL11_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNZ13626.1"
                     /translation="MPNRADEWMNLLQASGYRQTHARRAIVEILLAAECALEPMEIFE
                     AGRRQAPRLGLVTVYRTLEKLEELGLAQRVHQPQGCNRYLRAPQGHEHLIICTKCGRA
                     VYFQGDELDAFFGRVAEQSGFTVEDHWLQLFGRCARCQ"
     gene            1907..2848
                     /locus_tag="PKL11_08215"
     CDS             1907..2848
                     /locus_tag="PKL11_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ13627.1"
                     /translation="MSQSTSAHLTRGYVSAIFAAVFLSATAIFIRHLTLTYQLPALVL
                     AFWRDVFVVLTLLPVFALARPHLLRVSRQHLPYLVIYGFVLALFNSMWTLSVALNGAA
                     AATVLAYCSTGFTVLLGWLLLKERLNWGKLLAVSLTLGGCVLVSGAITREAWAINLGG
                     ILAGVLSGLCYAGYSLMGRSAAQRGLNPWTTLIYTFGFAAVFLLLVNLIPGGQIPGAA
                     SRPADFFWLGRAWVGWGILFLLAAIPTVGGFGLYNISLTHLASSVTNLIVSTEPVFTA
                     ITAYLLLGERLTGIQVAGSLLILGAVIFLRIYEGRQG"
     gene            complement(2968..3525)
                     /locus_tag="PKL11_08220"
     CDS             complement(2968..3525)
                     /locus_tag="PKL11_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ13628.1"
                     /translation="MTTPASPSKCAFCGAPLPADAKFCGACGQTVSVAAPAPAVVPPP
                     PPAEPVITPPAPPVNAMPGIPPTSTPNVTKPCPHCGQAISVDYERCPFCGGATKLEAP
                     APVPPATPAPVPPAPAYMPPPRPAAPAPAAPAEKKKLSTGCIVGIIIAVLLFCIGLCV
                     VGYLGYNYMADQGYLNLLPMLARLL"
     gene            complement(3652..4428)
                     /locus_tag="PKL11_08225"
     CDS             complement(3652..4428)
                     /locus_tag="PKL11_08225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13629.1"
                     /translation="MDPAELMAMFNHYQRKQVTWPGMLRQEVNGVVRQFAPEGGPAFI
                     EYSELDETTADAAIQQEIDFFTALGRGFEWKLYDSDRPADLQARLQARGFVLEDPEAL
                     VVLELSPDHPLLKQPIPPEICPVTDDAGVAGIQAMSEAVWNEERPWLAEQLKTDLRSH
                     PDRISIYAAWVDGRVVSAAWIYYHPGTPFASLWGGSTLAEYRGRGLYTGLLAARAQAA
                     RQRGYSLLYVDASPMSRPILEKHGFICLGFSRPCNFEPPA"
     gene            complement(4465..4779)
                     /locus_tag="PKL11_08230"
     CDS             complement(4465..4779)
                     /locus_tag="PKL11_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNZ13630.1"
                     /translation="MPALEPHPLADLDPLIHAPARLMILTYLYAVESADYVFLMRMTG
                     FTWGNLATHLGKLEEAGYVTLEKRFNGKKPQTLLRMTETGRAAFRAYKKAMQQVLDDL
                     PE"
     gene            complement(4781..5449)
                     /locus_tag="PKL11_08235"
     CDS             complement(4781..5449)
                     /locus_tag="PKL11_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13631.1"
                     /translation="MQDPISVKAAEQKAFRATLADGLWEVLAGCVALEFAVAPLLTKS
                     LGDFWSSAIFLPFWALVYLAILWVRRKVVAPRLGQVTFGPARKQRLRQVLLVIAVVTG
                     AALIFGILVLILFSGAAATPQDGPRLFLQGLAGWPLSLLGLCAFCIAGALLDYPRAYF
                     YGILVFMAPLAGEWLSERLGAAHHGFPIVFGLIAGLMIVLGLVTFFRWLQANPPLDLP
                     AEER"
     regulatory      5741..5849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5884..6528
                     /locus_tag="PKL11_08240"
     CDS             5884..6528
                     /locus_tag="PKL11_08240"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNZ13632.1"
                     /translation="MKNQRALLFVNGELPDPRQVAALIQPDDFLVAVDGGLEHLKALH
                     LQPHLLIGDLDSVDPQDVTRLEAAGVPVERFPVEKDENDLELALDSVVRRGCRTLRII
                     AGVGGRLDQTLANLFLLARPHLADCDLRLDDGQTEVLLVRQSAQIEGQPGETVSLLPL
                     GRPACGVTTLGLRYPLHAETLWPDRTRGVSNALAEPRASVRLSEGLLICVHIRG"
BASE COUNT         1192 a         2044 c         2131 g         1236 t
ORIGIN      
        1 tgccgctgct gccgctggtg ctgctgtttg catccctcaa cgccttcaac gaagagatga
       61 cctaccgggc ctcgctgctg gctccgctgg aaaatgtcgt gggctacaaa catgccctgg
      121 gcatcacggc ggccttcttt gggctgggcc acttctacgg cgtgccttac ggcctggtgg
      181 gcgtggccat ggcaaccttc ctgggctggc tgctgggcaa agccatgctg gagacgcgcg
      241 gcttctgctg gtcgtggctc attcacttcc tgcaggatgt gctgattttc tcctttatgg
      301 ccatcggctc gattgtgccg ggcggctaaa tgaggtccta gcgcagcgcc gtcacaatct
      361 gcgtgacgtt gaatcgcagc atctcaatat aggtgggtgc ctcgccgcct ggcggggtca
      421 gtgagtgggt gtacagcccg gtgaccacct gtgcgccggt ttcggcagcc acctgttcgg
      481 ccaactgcgg gttgacgccc tgctcgagga aaatggcggg cgcgcccgtg gcgcggatct
      541 gatcaaccag ttcggctacc tgctgggctg agggcgccgc ccccgcgctg acactggtag
      601 ccagcgcccc gaccaccgtc agaccgtagc ggtcggcaaa atagccaaag ctctcgtggt
      661 tggtcaccag cagccgccgc tccggcggga tggtctctat ttgttcccga atccaggcat
      721 ccagcgcctg aagttcggcg gtataggcgg cggcattggc ggcgtaaagc tcgccgccgg
      781 cagggtcggc tgccgtcagc ccggcccgga ttgtctcaac gtaggccatt gccaggttgg
      841 ggtccagcca gaaatgtggg tcgccctcgg tgtgcccgtg ggcgtcggcc tccaccgggc
      901 ggctttccag cccggccgcg gcctcaatca acagacgctc gccgtcggca ttctccagca
      961 cgggcagcag cgcctcttcc agcccggcgc cgttgaggat cagtacatcg ctgtcggtaa
     1021 tcaacgccag gtcttgcggc gtgggggtaa aggtgtgcgg gtccacgccc gcggggatca
     1081 gcgcggtcac ggtcaggcgc tcgccggcaa tgttctgggt gatgtctgcc agaaaggcct
     1141 cggccgccac cacgcgcagc ggctggtcgg cggcaggagg ggtggcggtg ggcgtgcagc
     1201 ccgccagggc aaacatcaga ccggccataa tgaaaatgaa accctttttc atttataaac
     1261 ctccagaaaa aattatcaaa agttgagcct gcaactgccc tactggcagc gggcgcagcg
     1321 cccaaacaac tgcagccagt gatcttctac cgtaaagcca ctctgctcgg ccacgcggcc
     1381 aaaaaaggca tccagctcgt cgccctgaaa atagaccgcc cgcccgcatt tggtgcagat
     1441 gatcagatgc tcatggccct ggggggcgcg caggtagcgg ttgcagccct gcggctggtg
     1501 cacccgctgc gccaggccca gttcttccag cttttccagc gtgcggtaca ccgtcaccag
     1561 gcccaggcgg ggggcctgcc gccgcccggc ctcaaagatt tccatcggct ccagggcaca
     1621 ttccgccgcc agcagaatct ccacaattgc ccggcgggcg tgggtctggc ggtagccgct
     1681 ggcctgcagc agattcatcc attcatcggc acggtttggc atgcaagcat cctccggtaa
     1741 tgaaaatcgt tttcaattat acccaggttt tttccctttg caaatggatg gcagggttgg
     1801 gaattgggtt tttgggggat tgccgcaccc ggaacctgga acatggaacg aacctttgac
     1861 ccctccgccc acgacaagaa acccggcctc gggtagaatt gggggcatgt ctcaatctac
     1921 ctctgcccac ctgacgcgcg gctatgtgtc cgccattttt gccgcggtgt tcctttcggc
     1981 cacggccatc ttcatccgcc acctcaccct cacctaccaa ctgccggcgc tggtgctggc
     2041 cttctggcgg gatgtatttg tggtgctgac ccttctcccg gtctttgcgc tggcccgccc
     2101 gcacctgctg cgggtttcgc ggcagcacct gccttacctg gtgatttacg ggtttgtgct
     2161 ggcgctcttc aattccatgt ggacgctctc ggtcgcgctc aacggcgcgg ctgcagccac
     2221 cgtgctggcc tactgctcca ccggctttac ggtactgctg ggctggctgc tgctcaaaga
     2281 gcgcctgaac tggggcaagc tgctggctgt ctcgctgacg ctgggcggct gtgtgctggt
     2341 ttcgggggcc atcacgcggg aggcctgggc catcaacctg ggcgggattt tggccggggt
     2401 gctctcgggg ctctgctatg ccggctacag cctgatgggc cgctcggccg cccagcgcgg
     2461 cctcaacccc tggaccacgc tgatctacac ctttgggttt gcggccgttt tcctgctgct
     2521 cgtcaatctc atcccgggcg ggcagattcc cggcgcggcc agccgcccgg ccgatttctt
     2581 ctggctgggg cgggcctggg tgggctgggg gattctcttt ctgctggcgg ccattcccac
     2641 agtgggcggc tttggcctgt ataacatcag cctgacccac ctggcctcca gcgtcaccaa
     2701 tctgatcgtc agcaccgagc cagtgttcac cgccatcacc gcctacctgc tgctgggcga
     2761 gcggctgacg ggcatccagg ttgccggcag cctgctgatt ctgggcgccg tgatctttct
     2821 gcggatttat gaggggcggc aggggtaggg cgcccggcat caccttaaaa tgacactggg
     2881 cgcaaagatt taattctttg cgcccaggtg acaggcacac caaacagccc ggcaggccgg
     2941 gaaggatgat ctaaaaatgg ggccgggtta caacagccgg gccagcatgg gcagcaggtt
     3001 gagatatcct tgatcagcca tgtagttata gcccagatag cccaccacgc acaggccgat
     3061 gcagaacagc agcaccgcga tgatgatgcc cacaatgcag ccggtgctga gctttttctt
     3121 ctcagccggg gcggccgggg ctggcgctgc cgggcgaggc ggaggcatgt aggccggggc
     3181 cggcggaacc ggcgcggggg tcgccggtgg cacgggtgcc ggagcttcca gcttggtggc
     3241 gccgccgcag aacgggcagc gttcataatc caccgagatg gcctggccgc agtgcgggca
     3301 gggtttggtc acattggggg tggaggtcgg cgggatgccc ggcatggcat tgaccggcgg
     3361 ggccggaggc gtgatcaccg gctcagcggg cggcgggggc ggcacaacgg ccggggcggg
     3421 cgcagccacg ctgacggttt gaccgcaggc gccgcagaat ttggcgtcgg cgggcagcgg
     3481 cgcgccgcaa aaagcacatt tggaggggga ggctggtgta gtcatgcgct gattctagca
     3541 taaaacgccg caagggaagc ccagaacaac cgggccaatt gcggttcaaa gcgggagaag
     3601 tctacgccag gggaccgaat cctcacaggg tactgcgcac caggccggtc ctcaggctgg
     3661 gggctcaaaa ttgcaggggc gcgagaaccc caggcagatg aacccatgtt tttcgaggat
     3721 gggacggctc atcgggctgg cgtccacata gagcagcgag tagccgcgct gccgggcagc
     3781 ctgggcccgg gcagccagca ggcccgtgta cagcccgcgg ccgcggtatt cggccagggt
     3841 ggagccgccc cacagactgg caaacggcgt gccggggtga tagtagatcc acgcggcgct
     3901 gaccacgcgg ccatctaccc aggcagcgta aatgctgatc cggtcagggt ggctgcgcag
     3961 gtcggtctta agctgctcag ccagccaggg acgctcctcg ttccagaccg cctcagacat
     4021 tgcctgaatg ccagccaccc cggcgtcgtc cgtcaccggg caaatttccg gcgggatggg
     4081 ctgcttcagc agcggatgat cgggggataa ttccagcacc accagcgcct cggggtcttc
     4141 gagcacaaag ccgcgggcct gcagccgcgc ttgaaggtcc gccgggcggt cgctgtcgta
     4201 cagcttccac tcaaaaccgc ggcccagcgc ggtgaaaaag tcgatctcct gctggatggc
     4261 ggcatcggcg gtggtctcat ccaactcgga atactcgatg aaggccgggc cgccctcggg
     4321 ggcaaactgc cgcaccacgc cgttgacctc ctgacgcagc atgccgggcc aggtgacctg
     4381 tttgcgctga tagtggttga acattgccat caattcggct ggatccatgg gggcctctcg
     4441 ggttcgggat gtttggattg tacgctattc tggcagatcg tccaagacct gctgcatggc
     4501 ttttttatac gcccggaaag cggcgcggcc ggtttcggtc atgcgcagca aggtctgggg
     4561 cttcttgccg ttgaagcgct tttccagggt gacatagccg gcttcttcca gcttgcccag
     4621 gtgcgttgcc agattgcccc aggtgaagcc ggtcatgcgc atcaagaaca cataatcggc
     4681 gctctccacg gcatataaat aggtcaggat catcaggcgg gcaggggcat ggatcaacgg
     4741 gtcaaggtca gccagcgggt gaggttcgag ggcgggcatg tcagcgctcc tcggcaggca
     4801 ggtcgagcgg cgggttggcc tgcaaccagc ggaagaaggt caccagcccc agcacgatca
     4861 tcaggccggc gatgaggccg aaaacaatcg ggaagccgtg atgcgccgcg cccaggcgct
     4921 ctgagagcca ttcgccggcc aggggcgcca tgaagaccag aatgccgtag aaataagcgc
     4981 ggggataatc gagcagtgcg ccggcaatgc agaaggcgca caggcccaga aggctcaacg
     5041 gccagcccgc caggccttgc aggaacagcc gcggcccgtc ttgcggcgtt gcggcggcgc
     5101 cggaaaagag gatcagcacc aggatgccaa agattagcgc ggccccggtg acgaccgcaa
     5161 tgacgagcaa aacctggcgc aggcgctgtt tgcgggccgg gccaaaagtc acctggccca
     5221 gccgcggcgc cacaaccttg cggcgcaccc agaggatcgc cagatagacc agcgcccaga
     5281 agggcaggaa gattgctgag ctccaaaagt cacccagcga cttcgtcagc agcggcgcca
     5341 cggcaaattc cagcgccaca cagcccgcca gcacctccca caggccgtca gccagggtgg
     5401 cccggaaagc cttttgctcc gccgctttga cactgatggg gtcttgcatt tcatactcct
     5461 ttgaactgcg ctcaaaaagt actttttatt acaaagtact tttatactat acggcaattg
     5521 tcaggaaaag tcaagtactg aaaaaggggg gataagtttc acgtttcacc tttcaggttc
     5581 cacgtgtaac ggtagctcgc attggatttt taggggcaag gccaggtgct ccgggacatc
     5641 actgaataga atcggcctcg ccctgacatt gcacaaagag gcgagaaaaa tcttctccct
     5701 tttttgacat ttcacgcaaa gtgcgttaca ctatctttaa ttaaacaaca cggggagcct
     5761 gcctggcagg ctgagaggag ggctttggtc ctcgacccgc attacctgat ccggattatg
     5821 ccggcggagg gacgttgatc agttggttca ccagacaccc tccccggcga gggtgttttg
     5881 cttatgaaaa accaacgtgc cttactcttt gtcaatggtg agctgcccga cccccgccag
     5941 gtggcggcgc tcatccagcc cgatgatttt ctggtggcgg tggatggcgg cctggagcat
     6001 ctgaaggcgc tccacttgca gccccacctg ctgatcggcg acctggactc ggtggacccg
     6061 caggatgtga cccgcctgga ggctgccggc gtgcccgtgg agcgctttcc ggtggagaag
     6121 gatgagaacg acctggagct ggccctggac tcggtggtcc ggcggggctg ccgcacactg
     6181 cgcatcatcg ccggcgtggg cgggcggctc gaccagaccc tggccaacct cttcctgctg
     6241 gcacgccccc acctggccga ctgcgacctg cgcctggacg atggtcagac cgaggtgctg
     6301 ctcgtccgcc aatccgcaca gattgaaggc cagcccggcg agaccgtctc gctgctgccg
     6361 ttgggacgcc cggcctgcgg cgtgaccacc ctgggtctgc gctaccccct gcatgccgaa
     6421 acgctttggc ccgaccgcac ccgcggcgtc agcaacgccc tggccgagcc gcgcgccagc
     6481 gtccgcctga gcgaagggct gctgatttgc gtccacatca gggggtagga gttgggcgtt
     6541 aggaattaga tctcggattg aaagaagcaa cgtggtacgt ggaatattga acgaacctga
     6601 aac
//
LOCUS       DAOPVS010000102         6601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_11583_length_6601_cov_54.875191, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000102 DAOPVS010000000
VERSION     DAOPVS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11583_length_6601_cov_54.875191"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PKL11_08245"
     CDS             complement(<1..1309)
                     /locus_tag="PKL11_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13633.1"
                     /translation="MTETIDHDAIRAHIQKNYRWNFLVNTLDEAYYSLGMSFFSTAIV
                     LPLFVSHFTSSPLAIGLIAFLGWAGTLLPPLFVANRVERAPIKKIFPVGVGFFAELLP
                     MLLLAPVIYLLSDSRAGLTLAVFFFLFAWANFGDGVILVGWEDMIAKIIPTEKRGRFF
                     GITNFVGGLTGLAGALAVTWVLDMLPFPLAYIFCFGAAGVLSFIAWLFAALTREPAVP
                     SQKPPVSQLAYLRSLPGVLRRDQNFRMYLISQIIFALSGMATGFLAVYSVQRWGLTDA
                     KASGFLIAMQAGLMAANLLLGFLSDRKGHKLSLEICFGFNLLALVLAVIAQGPLWFYV
                     IFFLRGVADSGLAISGVAIVYEFTNAENRPTYLGLANTIPGLHMVIAPLLGGWLAGAA
                     GYPAMFGISTLIGVVSWGLLHYTVREPRLKTISPPTVPISYARI"
     gene            1440..>1888
                     /locus_tag="PKL11_08250"
     CDS             1440..>1888
                     /locus_tag="PKL11_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNZ13634.1"
                     /translation="MNTPLSPEQREELLRRLKTRFEKNMSRHAGLEWADVQSRLDADA
                     EKLWSLNEMEKTGGEPDVIGRDEKTGQYIFCDCAAETPRGRRSVCYDRAAQESRKDFA
                     PENNALDVAAAMGIEILTEDGYRALQALGEFDLKTSSWLATPPEIRAL"
     assembly_gap    1889..1988
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1989..2471
                     /locus_tag="PKL11_08255"
     CDS             <1989..2471
                     /locus_tag="PKL11_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ13635.1"
                     /translation="DYANDFSDPALGAQLAQDQMAAGADVIFGVGGPMGNGAILEAAQ
                     SGVWVIGVDADAWDMVFGGGTVTGSDYLLTSALKRVDTAVYDTIADQIGGTFTSGTAL
                     YDLSNDGVGLAPYHAAETAIPQEVKDAVNAAAEGLLNGTIDPWLQPDWQYIFLPVTLK
                     "
     gene            complement(2566..>3258)
                     /locus_tag="PKL11_08260"
     CDS             complement(2566..>3258)
                     /locus_tag="PKL11_08260"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13636.1"
                     /translation="APPLDVLTDISFGLEAQEFVAVLGPSGSGKSTLLRVLAGLLPPT
                     AGSAEFCSDLPIRVGLVFQQANLMPWRTVLENITLPLELQGVPVETACVRAREWVAQV
                     GLQGFEDAWPRDLSGGMAQRVALARALIADPDLLLLDEPFGALDALTRERMGAELLAL
                     WQARRKTVLLVTHSISEALLLSDRVLVLTQRPGRICLDLSVPFPRPRTEELRYTPEFG
                     ALSRRLRAAIGN"
     assembly_gap    3259..3358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3638..4618)
                     /locus_tag="PKL11_08265"
     CDS             complement(3638..4618)
                     /locus_tag="PKL11_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ13637.1"
                     /translation="MKKFAWMLLLLTATLLAGCITPTPTAALTPVKLPLGYIPNVQFA
                     PLYVALEKGYFAAEGLDVSLDYSMENDNTALVGAGQLDFALVSGEQVLLGRGQGLPVV
                     YVLAWYGQYPVGVVFKSGQNIQTPADLKGHTIGIPGLYGASYIGLRALLSAGGLTEAD
                     VKLESIGFTQVEAVATDQVEVGVIYLANEPNQLRTQGYDVATLSASDYVGLVSNGLIT
                     SEKTLAENPELVTKMVRAILRGTADAAADPDGAYEICKKYVENLAQADETVQKQVLAD
                     SIAMWQLDPPGVTDPAAWESMHALLLDMGLLSAPLDWNTAFTNQFVSGEK"
     gene            complement(4649..5449)
                     /locus_tag="PKL11_08270"
     CDS             complement(4649..5449)
                     /locus_tag="PKL11_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13638.1"
                     /translation="MFVPDEADLKPLVVKLLKRYGRWLPLISAGLALLIWQLVSQFGG
                     LPAFILPSPLAVAARFWKALVEGALLRHVGVTLLEVVLGLLAGSLMAMFLGYWLARSR
                     LLEKVLSPYLVASQAIPIVAVAPLLVIWFGPGMFSKVLICALTVFFPVLVNTVVGLRA
                     VPENLRDLMRALRASRGQMLRHLEIPAALPVFLGGLRVGATLSVIGAVVGEFVGADRG
                     LGFLINVGRGQYDTALVFAAIFTLIALAMLLYGAVLLLERRLLRWRRE"
     regulatory      complement(5506..5635)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5943..6575
                     /locus_tag="PKL11_08275"
     CDS             5943..6575
                     /locus_tag="PKL11_08275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNZ13639.1"
                     /translation="MPTLDWIGKAAVRNHHLEVPFHLLRADAALSCGAPAAGNLLIEG
                     DNLLALKALLPYYAGQVKCIYIDPPYNTGNEQWVYNDNVNSPEMRTWLGRVVGGEAED
                     LSRHDKWLCMMYPRLTLLRQMLREDGVILISIDDNEAHSLRMLLDEIYGRSNFVGTIV
                     WRNVTDNNPTRISIEHEYVLCYARDKAKLESQWKATALPIKDLNTGHFNK"
BASE COUNT         1404 a         1998 c         1925 g         1074 t
ORIGIN      
        1 tgatgcgggc gtaactgatc ggcaccgtgg gcggcgagat ggtcttcagg cggggctcac
       61 gcacggtgta gtgcagcagc ccccagctca ccacgccaat gagggtcgag atgccaaaca
      121 tggccgggta accggcggcc ccggccagcc agccgccgag cagcggggca atcaccatat
      181 gcaggcccgg gatggtgttg gccagcccca ggtaggtggg gcggttttcg gcattggtga
      241 actcatacac aatggccacc cctgaaatag ccaggccgga atcggccacg ccgcgcagga
      301 agaaaatcac gtagaaccag agagggccct gcgcaatcac ggccagcacc agcgccagca
      361 ggttgaagcc aaagcagatc tccaggctca gtttgtgccc cttgcggtcc gataaaaagc
      421 ccagcagcag gttggcagcc atcaacccgg cctgcatggc aatcagaaag ccgctggcct
      481 tggcgtcggt caggccccag cgctgcacgc tgtacaccgc caggaagccg gtggccatgc
      541 cgctcaacgc aaagatgatt tgcgagatca ggtacatgcg gaagttttga tcccgccgca
      601 gcacgccggg cagcgaacgg aggtaagcca actgcgagac gggcggcttc tggctgggca
      661 cggctggctc acgcgtgagg gcggcaaaca accaggcaat gaaagacagc acccctgccg
      721 cgccaaaaca aaagatgtag gccagcggga agggcagcat atccagcacc caggtgaccg
      781 ccagcgcgcc cgccagcccg gtgaggcccc ccacaaaatt ggtgatgccg aaaaagcgcc
      841 cgcgcttctc ggtagggatg attttggcaa tcatatcttc ccagcccacc agaatcacgc
      901 catcgccaaa attggcccag gcaaacagga agaagaagac cgccagcgtc aacccggcgc
      961 ggctgtcgga cagtaagtaa atgaccggcg ccagcagcag catgggcagc agctcggcaa
     1021 agaaaccgac gcccaccgga aagatctttt tgattggggc gcgctccacc cggttggcaa
     1081 caaacagcgg gggcagcaga gtaccggccc agcccaggaa ggcaatcagg ccgatggcca
     1141 gcgggctgct ggtaaaatgg ctgacaaaca gcggcagcac aatggccgtt gagaagaaac
     1201 tcatccccag tgagtagtaa gcctcgtcca gtgtgttgac cagaaaattc cagcgataat
     1261 ttttctggat gtgggcccgg attgcgtcgt ggtcaatcgt ctcggtcaaa ggggagaaca
     1321 cctttaaaat atatttgtga tggcggtatt atacaaccaa cccgcgcctt tgcgttgaaa
     1381 tcctgcctat ttgcccaccg gcgcttgact tgcccctgcg acagcgataa gataaaagca
     1441 tgaatacccc tttatctccc gaacaacgtg aagaactgct ccgcaggttg aagacccgct
     1501 ttgagaaaaa catgtcccgc catgccggtc tggaatgggc tgatgttcag tcccggctgg
     1561 atgccgacgc tgagaaactg tggtccctca atgagatgga aaaaaccggc ggcgagcccg
     1621 atgtgattgg ccgcgacgaa aagacgggcc agtacatttt ctgcgattgt gctgccgaaa
     1681 cccccagggg ccgccggagc gtgtgctatg accgcgccgc gcaggaatcc cgcaaagact
     1741 ttgcacccga aaataacgcc ctggacgtgg ccgcggccat gggcattgag attttgaccg
     1801 aagacggcta ccgggcgctg caagcgctgg gcgagtttga tttgaaaacc tcgagctggc
     1861 tggcaacccc acccgagatt cgcgccctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnga ttatgccaac gatttctcag acccggctct gggggcgcag ttggcgcagg
     2041 atcaaatggc cgccggggcc gatgtgatct tcggcgtggg cgggcccatg ggcaacggtg
     2101 ccattctgga ggctgcccag tccggcgtct gggtgattgg cgtggacgca gacgcctggg
     2161 acatggtctt tggcggcggc acggtgaccg gcagcgacta cctgctgacc agcgccctga
     2221 agcgggttga taccgccgtg tatgatacca tcgccgacca gatcggcggc accttcacct
     2281 ctggcacggc gctgtacgat ctgtccaatg acggcgtggg cctggcgccc tatcacgctg
     2341 ccgaaactgc catcccccag gaggtgaaag atgcggtgaa tgccgctgca gagggcctct
     2401 tgaacggcac cattgacccg tggctgcagc cggactggca gtatatcttc ctgcccgtca
     2461 cgttgaagta acccggtaca gtcagcgccg gcccattggg ccggcgctgt ttattatgct
     2521 gtgggatcgg gtcagcttga acccgctcac ctgcaaaact tgaatttaat tgccgatggc
     2581 ggcgcgcagg cggcgagata aggctccaaa ttccggggtg tagcgcagtt cttcggtgcg
     2641 cgggcggggg aagggtacag acagatccag gcagatgcgc cccgggcgct gggtgagtac
     2701 cagcacacgg tctgaaagca gcagcgcctc tgagatggag tgcgtgacca gcagcaccgt
     2761 tttgcggcgg gcctgccaca gcgccagcag ttcagcgccc atgcgctcac gggtcagggc
     2821 gtccagtgcg ccaaagggtt catccagcag cagcaggtcg gggtcggcaa tcagggcgcg
     2881 ggccagcgcc acccgctggg ccatcccgcc cgaaaggtcg cgcggccagg cgtcttcaaa
     2941 cccctgcagg cccacctgcg ccacccattc gcgcgcccgc acacaggccg tttctacggg
     3001 cacgccctgc agttccagcg gcagggtgat gttctcgagc accgtgcgcc agggcatcag
     3061 gttggcctgt tggaacacca gccccacccg gatgggcagg tctgagcaga actcggccga
     3121 gccggccgtg ggcggcagca gccccgccag cacccgcagc agggtgcttt tgccgctgcc
     3181 cgatggcccc agcacagcca caaactcctg cgcttccagg ccaaagctga tgtcggtcag
     3241 cacgtccagc ggcggcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     3361 tcctgcgctt ccaggccaaa gctgatgtcg gtcagcacgt ccagcggcgg cgctccgtcg
     3421 ctggatggga aggttatact gaggtggtca acggtcaggg cttccatttg aaccctcacc
     3481 tggcctctcc ctgaaaggga gaggagaaaa gaaggggtct ctccctgaaa cggagaggag
     3541 aaaagaagat gcctctccct gtacgggagg gggaaaagaa gaggtctctc cctgtacggg
     3601 agaggagaaa agacgccgga cggtacaaac ggcacatcta tttttcgcca gacacaaatt
     3661 gattggtgaa ggcggtgttc caatccagcg gggccgagag cagccccatg tcgagcagca
     3721 gggcgtgcat gctctcccag gcggcggggt cggtcacgcc gggcgggtca agctgccaca
     3781 tggcgatgga atcggccagc acctgctttt gcacggtctc atccgcctgg gccagatttt
     3841 caacgtattt tttacaaatc tcataggccc catccgggtc ggcggctgcg tcggctgtgc
     3901 cgcgcagaat ggcccgcacc atcttcgtca ccagttcggg gttctcggcc agcgtctttt
     3961 cgctggtgat cagcccgttg gagaccagcc caacataatc tgacgcgctc agggtcgcca
     4021 catcgtaccc ctgcgtgcgc agttgattgg gctcattcgc caggtagatc acgccgacct
     4081 ccacctggtc ggtggccacg gcctccacct gggtaaagcc aatcgattcc agcttcacat
     4141 cggcctcggt cagcccgccg gcgctcagca gggcgcgcag accaatatag ctggccccgt
     4201 acagccccgg aatgccaatg gtgtgcccct tgagatccgc cggggtctga atattctgcc
     4261 cgctcttgaa gaccacgccc accggatact gcccgtacca ggccagcaca tagaccaccg
     4321 gcagcccctg gccgcggccc agcagcacct gctcgcccga aaccagggca aaatccagct
     4381 gcccggcgcc caccagggcg gtgttatcgt tttccatgct gtaatccagg ctcacatcca
     4441 gcccttcggc ggcaaaatag cccttttcca gggccacata cagcggggca aactgcacat
     4501 tggggatgta ccccagcggc agcttgacgg gcgtcagcgc ggcggtgggg gtgggggtga
     4561 tgcatccggc cagcagggtg gcggtcagca agagcagcat ccaggcgaat tttttcatgt
     4621 aaacctcact caaaaaaaga ttgaaaaact attcccggcg ccagcgcagc aggcggcgct
     4681 ccagcagcag caccgcgccg tacagcagca tggccagcgc aatcagggta aaaatggccg
     4741 caaagaccag ggccgtatca tactgcccgc ggcccacgtt gatcagaaat cccagcccgc
     4801 ggtcggcgcc cacaaactcg cccaccaccg cgccaatcac cgacagcgtg gcgcccaccc
     4861 gcagcccgcc cagaaaaacc ggcagcgccg ccggaatctc caggtggcgc agcatctgcc
     4921 cgcggctggc gcgcagggcg cgcatcaggt cgcgcagatt ttcgggcacg gcgcgcagcc
     4981 ccaccaccgt attgaccagc accgggaaga agaccgtcag cgcacagatc aacaccttgg
     5041 agaacatgcc cggcccaaac cagatgacca gcagcggggc cactgccaca atggggatgg
     5101 cctgactggc aaccaggtag ggcgagagca ccttctccag caggcgcgag cgggccagcc
     5161 agtagcccag aaacatcgcc atcaggctgc cggccagcag gcccagcacc acctccagca
     5221 gggtaacccc cacatggcgc agcagcgcgc cctcaaccag cgccttccag aagcgcgccg
     5281 ccaccgccag cggcgacggc aggatgaacg ccggcagccc gccgaactga ctgaccagct
     5341 gccagatcag cagcgccagc ccggcagaaa tcagcggcag ccagcggcca tagcgtttga
     5401 gcagttttac aaccagcggt ttcaggtcag cctcatcagg aacaaacaaa aagccccaaa
     5461 gcgggcgctt cgggggcggg gcagccgcgc atcccggggg acgcggctgg ccttctttca
     5521 tccggactgt accgtcggtt ccggaatttc accgggtcgt gcgcaggcgc ttgtggactg
     5581 tgaccaccga tcgggaattg ggacgcatgt cccgcaccct gccccgaagg caactcaaaa
     5641 gattgacgct gcaaagtata tgcgcaccgg ggcaatttgt caaatccagg ggatggcacg
     5701 cgccgggcca tcgtttgcga aattaacgca ggcggggcaa tcagcgccgc ccgtaccggg
     5761 gattaatgaa tcagaagcca agccctcttc cctcgccgct tcgacactct gtagagaaat
     5821 caggcgctgg ccccgagtca ggtcaaagcc tggcgcagcc tgacgataag ccgcgcccgt
     5881 ttgtgtgtat aattaagcta ttgggtggac gccctaaaat cttcaacccc ccggatacgc
     5941 tcatgcccac actcgattgg attggtaaag ccgccgtccg caatcaccac cttgaggtgc
     6001 cctttcattt gctgcgcgcc gatgcggcgc tgtcttgcgg cgcgcccgcg gcgggcaacc
     6061 tgctgataga aggcgacaac ctgctggccc tcaaggccct gctgccctac tacgccgggc
     6121 aggtcaagtg catttatatt gacccgccct acaacaccgg caacgagcag tgggtttata
     6181 acgacaatgt gaacagcccc gaaatgcgca cctggctggg ccgcgtggtg ggcggcgagg
     6241 ccgaagacct ttcgcgccac gataagtggc tgtgcatgat gtacccccgc ctgaccctgc
     6301 tgcgccagat gctgcgcgag gacggagtta ttttaatctc aatagatgat aatgaggccc
     6361 attctctccg tatgcttctg gatgaaattt atggacgctc aaattttgtc ggtactattg
     6421 tttggcgaaa tgttactgat aataacccta ctcgcataag tattgaacac gaatatgtac
     6481 tttgctatgc aagggacaaa gccaaattag aaagtcaatg gaaagcaacg gcacttccaa
     6541 tcaaagattt gaataccgga cactttaaca aatgacgact gtcataaggt acactatgtt
     6601 t
//
LOCUS       DAOPVS010000103         6515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_11772_length_6515_cov_52.840712, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000103 DAOPVS010000000
VERSION     DAOPVS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_6515_cov_52.840712"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..2122
                     /locus_tag="PKL11_08280"
     CDS             65..2122
                     /locus_tag="PKL11_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNZ13640.1"
                     /translation="MDSGKKWLIAIIAIVLILCCCLLACLGVGYWAYKEGSNILPTDF
                     VDYWPTESPAFNTPSEWGNTTPVPPGSTPAPMVTSAPASSGAWETLGILEESVVPFNN
                     PVELAERLEGKANIPETVPATPAQVGDRREFWVTNVDTNENFRITAVMQYETDHVYFW
                     IEEGVSFDEGDLESLVESFENDIYPTNREFFGSEWSPGVDSDPHLYILYAGGLGWNLA
                     GYFSSADEVHISAHEYSNMHEMFLLNSDNVRLEEEFTYGVLAHEFQHMIHWKTDRNEE
                     SWLNEGFSELSALLNNLDPGGFDYSFASDPDLQLNDWPNDPDATTPHYGAGFLYTAYF
                     LDRFGEDATKALVAHPDNGMDSVDLVMQELNITDPLTGKLINAEDIFGDWVVANYLQD
                     GSVSDGRYTYHNYNNVPNFNATETISDCSSASWNDRSVSQYGTDYIALECSDNVTLEF
                     VGATTAAVLDTEAHSGDYAFWSNKGDESDMRLTREFDFTGVSAPIELNYWTWYDLEED
                     YDYLYLVASTDGGETWQIINTPSCTTYDPSGNSFGCGYNGVSDWKQETVDLSDFAGQK
                     VQLRFEYITDAAVNGEGFLLDDMEIPAINYTTDFETDDGGWLAEGFVRIQNQLPQTFR
                     VSLISNGGETTVQTIELDETQSFSLPLNFDGDIDDAVLVVSGTTRFTRQPAIYRFRLV
                     QTP"
     assembly_gap    2422..2521
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2522..5361
                     /gene="purL"
                     /locus_tag="PKL11_08285"
     CDS             <2522..5361
                     /gene="purL"
                     /locus_tag="PKL11_08285"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNZ13641.1"
                     /translation="VRFTAADPRSRALLADAHALGLTRIHNLACHDLFFVKGSLLPSD
                     LDRLGQALLCDGITQQFQVEPLEALPVIHPGDLILEVALRPGVTDPVVEQLVRAAAEI
                     GVPGLDQVATGQRYLVSGDNLSEADLHLLAQRLLANDVIQRYALGCIQPNFPAVAQSS
                     GAVEQIPLRNLTDDQLLALSQDRRAALNLPEMQAIQAWFTAAGRDPTDIEFEMLAQTW
                     SEHCVHKTFKAHIHLDDGQIIPGLLRTYLRSVTESIAAPWVLSAFVDNAGIIEYDGQH
                     EVSFKVETHNHPSAIEPFGGANTGVGGVIRDVMGVSARPIAATDVLCFGPQSADPETL
                     PAGVLHPRRIQSGVVAGVQDYGNKMGLPTVSGAIWYDPGFTANPLVFCGCVGLAPLGR
                     HCRTPQPGDHVIVLGGRTGRDGLRGATFSSMTMDAQTGEVSGASVQIGNPIVEKCTAD
                     VLILARDAGLYHAVTDCGAGGLSSAVGEMAAECGADVDLQSVPLKYPGLAPWEIWLSE
                     AQERMVLAVPPQNLPALADLCNNYNVELTDLGVFTRTGRLVVRWGAEVALDLDVHFLH
                     DGIPLRHLPAVLPPTAPAAPVASHSDSRSPEEILLALLAHPNLSSREPIIRQYDHEVQ
                     GATVIKPLTGIHADAPSDACVLQPLETRGPVALALACGLNPEYGKLDPYRMACSALDE
                     AVRNVVAVGADPDRIAVLDNFCWGNPANPETLGQLVAAARGCHDAALLHKTPFISGKD
                     SLNNEYLGSDGLRHAIPPTLLISALGILSDVSHAVSMDLKSPGSRLYLVGQHAPHFGG
                     SHFALVGAPLTSDLDRSVPNLPPETPGVYHALHLAMTRHLVSACHDLSEGGLALAAAE
                     MCIGGGLGLTMTLPTGNSTGWLFGETNGCLLVEVSAAHASAFEALFTGLPICRLGQVT
                     AEPILHIEQAGRLVLASPIANLTLAWKGAAL"
     gene            5358..6134
                     /gene="purQ"
                     /locus_tag="PKL11_08290"
     CDS             5358..6134
                     /gene="purQ"
                     /locus_tag="PKL11_08290"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNZ13642.1"
                     /translation="MIPKVLILKASGTNRDGDAAAAFTLAGAEPHILPLAVLRTSKIP
                     WQDYDILVLPGGFSYADALGAGRLLALDLTAYFTDQAQAFVASGKPVIGICNGFQALV
                     KSGILPGLPADNGPAATLTFNESGHFECRWVALRSVSTRCLWTHDLGVLECPVAHGEG
                     RFVTRDTQTLAQLQSNDQIALVYASAAGDPAQGAYPANPNGSILDIAGICNPAGNVLG
                     LMPHPEDHIFPTRHPHWPRGEARGSGLTLFQNGVKFAQER"
     gene            6137..>6515
                     /locus_tag="PKL11_08295"
     CDS             6137..>6515
                     /locus_tag="PKL11_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNZ13643.1"
                     /translation="MPLLPFTETRLPLSDPQRGKVRDNYALSAERRLLVTTDRLSAFD
                     RILGALPYKGQVLNQLSAWWFEQTRSIVDNHVLSVPDPNALLAVAAEPFPVEVIVRGY
                     ITGVTTTALWYRYALGEREIYGYR"
BASE COUNT         1234 a         2010 c         1658 g         1513 t
ORIGIN      
        1 ggtaaaggcg cattttagag tataattttc accctgttaa ccgctttaat cactggagga
       61 tatgatggat tctggtaaaa aatggttgat tgccattatt gcaattgtgc ttattctgtg
      121 ctgttgtctg ctcgcctgtc ttggcgtagg atattgggcc tataaagaag gcagcaatat
      181 cctgcccacc gattttgtcg attactggcc gacggaatct ccggcgttca acacaccgtc
      241 ggaatgggga aacaccaccc ccgtaccgcc gggctccacc ccggccccca tggtgacatc
      301 cgctccggcc tcttccggtg cctgggaaac cctcggcatt cttgaagaat ctgtagtgcc
      361 cttcaacaac ccggttgaac ttgctgaacg gcttgagggc aaagccaaca ttcccgaaac
      421 ggtccctgcc accccggctc aggtcggcga ccggcgggag ttctgggtca ccaacgtcga
      481 taccaacgaa aacttccgta tcaccgcggt gatgcagtac gaaaccgatc atgtttattt
      541 ctggattgaa gaaggcgttt cgtttgatga gggcgatctg gaaagcctgg ttgaatcctt
      601 tgaaaacgac atctatccca ccaaccggga gtttttcggc agcgaatggt ctcccggtgt
      661 agacagtgac ccgcatctct acatcctcta cgcgggcggc ctgggctgga atctggctgg
      721 ctacttctcc tctgccgatg aggtccacat ttctgcgcat gaatattcca acatgcatga
      781 gatgttcctg ctgaattccg ataacgtccg cctggaagag gaatttacct acggcgtcct
      841 ggctcacgaa ttccaacaca tgatccactg gaaaactgac cgcaatgaag aatcctggct
      901 gaatgaaggc ttctcggaac tctccgctct gttgaataac ctggacccgg gtggttttga
      961 ttactcgttt gccagtgatc ccgacttgca gctgaacgat tggcccaacg accccgatgc
     1021 caccaccccg cattatggcg ctggcttcct ttacacggct tacttcctcg accgctttgg
     1081 cgaagatgcc accaaagccc tggtggccca tcccgataac ggcatggaca gcgttgatct
     1141 ggtcatgcag gaactgaaca tcaccgatcc gctgactggt aagctcatca atgccgaaga
     1201 tatctttggc gattgggttg ttgccaatta ccttcaagat ggctcggtct cagatggccg
     1261 ttacacttac cacaactaca acaatgtgcc caacttcaat gctaccgaaa cgatctctga
     1321 ttgctccagt gcttcctgga atgatcgcag tgtcagccag tacggcaccg actatatcgc
     1381 actggaatgt tctgacaatg tcacgctgga atttgtcggc gccaccactg ccgccgtcct
     1441 ggatactgaa gcccattctg gcgattacgc cttctggtca aataaaggcg acgagtccga
     1501 tatgcgcctc acccgggaat ttgatttcac tggcgtcagt gctccaattg agttgaacta
     1561 ctggacctgg tacgatctgg aagaggatta cgactacctg tatctggttg cttcgaccga
     1621 tggcggtgag acctggcaaa tcattaatac cccttcctgc accacttacg acccctctgg
     1681 caacagcttt ggctgtggct ataacggcgt ctcagattgg aagcaggaaa ccgtggatct
     1741 ttctgacttt gccggacaaa aagtgcagct tcgctttgag tacatcacgg atgccgccgt
     1801 caatggcgaa ggcttcctcc ttgatgacat ggaaatcccc gccatcaact acaccactga
     1861 ttttgaaacc gatgacggcg gctggctggc tgaaggcttt gtccgcatcc agaaccaact
     1921 gccccagacc ttccgtgtct cgctcatcag caatggcggc gagaccacag ttcaaaccat
     1981 cgaattagac gaaactcagt cattcagtct gcccctcaat tttgacggcg acattgacga
     2041 tgccgtgctg gttgtcagcg gcaccacccg cttcacccgc cagccagcca tctaccgctt
     2101 ccgtctcgtt caaacaccct gaactgcatc ccgattcgat cagtctcttt tggagggcgg
     2161 attattcatc cgccctcttt ttgtgctgga cccattcaat atcctgctta acaaacatgg
     2221 ataaatcgcg ctcaaactca tgacgtccat cggcagtcta tgattgaccg gataactttg
     2281 gcagggtaga attagttgta taaacaactg tgcggaggtg ttcatagtgg ataacattcc
     2341 aactcaaagc ggcgtctatc gttatgtcgt tcgctttacc gccgccgatc cgcgcagccg
     2401 cgccttgctg gcggatgctc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 ntcgttcgct ttaccgccgc cgatccgcgc agccgcgcct tgctggcgga tgctcacgcc
     2581 ctgggcctga cccgcattca taacctggcc tgccacgacc tgttctttgt caaaggcagt
     2641 cttctgccct cagaccttga tcgcctgggg caggctctgt tatgtgacgg cattacccag
     2701 cagtttcaag tggagccgct ggaagcctta cccgttatcc accccggaga cctgatcctc
     2761 gaagttgccc tccgcccggg cgtcactgac ccggtcgttg aacagcttgt ccgtgctgcc
     2821 gccgagatag gcgtccccgg gttggatcaa gtggctaccg ggcagcgtta cctggtctcg
     2881 ggcgataacc tctctgaagc cgatttgcac cttttagcgc agcgtctgct ggcaaatgat
     2941 gttatccagc ggtacgcgtt gggctgcatc cagcccaatt tcccggcagt ggctcaatcc
     3001 agcggcgcgg tggagcaaat tcctctgcgc aatctgaccg atgaccagct tttagccctc
     3061 tcacaggacc gccgcgcggc gcttaacctt ccagagatgc aggccattca ggcctggttt
     3121 acagccgctg gacgtgaccc caccgatatt gaatttgaaa tgctcgccca aacctggagc
     3181 gagcattgtg ttcacaaaac cttcaaggcc cacatccacc tggacgatgg tcaaatcatc
     3241 cccggcttgc tgcgtaccta cctccgctca gtcaccgagt ccatcgctgc accctgggtc
     3301 ctctccgcct ttgtggataa tgcaggcatc atcgaatacg atggtcagca cgaagtctct
     3361 tttaaggttg agacgcataa ccatccctcc gccattgagc cctttggcgg cgcaaatacc
     3421 ggtgtaggtg gcgtgattcg agacgtcatg ggggtttctg cccgacccat cgccgcaacc
     3481 gatgtgttgt gttttggccc ccaatctgcc gatcctgaaa ccttgcctgc tggcgtgttg
     3541 cacccgcgcc gcatccagtc gggcgtggtt gctggtgtgc aggattatgg caataaaatg
     3601 ggccttccca cggtcagcgg cgccatctgg tatgatccgg gctttaccgc caacccgttg
     3661 gtcttttgcg gctgtgtagg gctggccccg cttggccgtc actgccgcac cccgcagccc
     3721 ggcgaccatg tgattgtgct gggcgggcgc accggacgtg atggcctgcg cggcgctacc
     3781 ttctcctcaa tgacgatgga cgcccagacc ggcgaagtgt ccggcgccag cgtccagatt
     3841 ggcaatccca ttgtcgaaaa atgcaccgcc gatgtattga tcctggcccg ggatgccggg
     3901 ctgtatcatg ccgtgaccga ctgcggcgct ggtggactct cctctgctgt cggcgaaatg
     3961 gccgccgaat gtggcgccga tgttgatctg caatcggtcc cgctcaaata tcccggtttg
     4021 gccccctggg aaatctggct ctcagaggct caggaacgca tggtgcttgc cgttccaccg
     4081 cagaatttgc ccgctctggc cgacctttgc aacaactaca acgttgaact caccgacctc
     4141 ggcgttttta cccgcaccgg gcggctggtc gttcgctggg gcgccgaagt tgccctggac
     4201 cttgatgttc acttcctgca cgatggtatt cctctacggc atctgccggc agttttaccc
     4261 cccacagctc ccgctgcgcc cgtcgccagc cactcagatt cgcgctcccc ggaagaaatc
     4321 ttgctggcct tgctggcaca tcccaacctg tcttcccgtg agcccattat ccgccagtac
     4381 gaccatgagg ttcaaggcgc caccgtcatc aaaccgctga ccggcatcca tgccgatgcg
     4441 ccttcagatg cctgcgtcct tcagcccctg gagactcggg gcccggttgc ccttgcattg
     4501 gcctgcgggc tcaaccccga gtatggcaaa ctggatccct accgcatggc atgcagcgct
     4561 ttggatgaag ctgtccgcaa cgtcgttgcg gttggcgccg accctgatcg catcgccgtc
     4621 ctcgacaatt tttgctgggg taacccggcg aaccctgaaa ccctgggtca gttggtcgct
     4681 gcggcccgtg gctgtcacga cgccgctctg ctgcacaaaa cccccttcat ctccggcaaa
     4741 gactcgctga acaatgaata cctgggctct gatgggctgc gccatgctat cccacccacc
     4801 ttgttgatct ctgctctggg catcctgtca gatgtatccc acgccgtcag catggacctg
     4861 aaatcacccg gcagccgcct ctatctggtt gggcaacacg ccccacattt tggcggtagt
     4921 cattttgccc tggtcggcgc cccgttgacg tctgacctgg atcgctctgt gcccaattta
     4981 cctcccgaaa caccaggtgt ttatcatgcc ctgcatctgg ccatgacccg gcatctggta
     5041 agtgcctgcc acgatctcag cgaaggtggg ctggctctgg ccgctgccga gatgtgcatc
     5101 gggggcggcc tgggtttgac catgaccctg cccaccggaa attccaccgg ctggctgttt
     5161 ggtgaaacca atggctgcct gctggttgag gtttctgcgg cgcacgcctc tgcctttgaa
     5221 gccctcttca cgggtctgcc catttgccgg ctgggtcagg tcaccgctga acccatctta
     5281 cacattgaac aggctggtcg ccttgtctta gcctcgccaa tcgccaacct gaccctcgcc
     5341 tggaaaggag ccgcactgtg atccccaagg tactcattct taaagccagc ggcaccaatc
     5401 gagatggtga cgctgcggca gctttcaccc tggctggcgc cgagccgcat atcttgccgc
     5461 tggcagtttt gcgcacgtcc aaaatcccat ggcaggatta cgatattctt gtccttcccg
     5521 gcggcttctc gtatgccgat gcgctcggcg ctggccgcct cctggctctg gacctgaccg
     5581 cctattttac cgatcaggcg caggctttcg ttgccagcgg taagcccgtc attggtattt
     5641 gcaacggctt ccaggcgctg gtaaagtcgg gcattctccc cggcttgcca gccgataacg
     5701 gccccgcggc cacattgact ttcaacgaat ccgggcactt tgaatgtcgc tgggtggcac
     5761 tgcgctctgt ttccacccgc tgcctatgga cgcatgatct gggcgtgctg gagtgtcccg
     5821 tggcacacgg tgaaggccgc ttcgttacgc gagataccca aacccttgcc caattgcaat
     5881 ccaatgatca aatcgccctg gtttatgcca gcgccgccgg tgatcctgcg caaggcgcct
     5941 atccagccaa ccccaatggc tcaatcttgg atattgctgg catttgtaac ccggctggca
     6001 acgtgctggg cctgatgccg caccctgaag atcatatttt cccaacccgc cacccacact
     6061 ggcctcgggg tgaagcgcgc gggtcgggcc tgaccttatt tcaaaatggc gttaagttcg
     6121 cccaggagcg ctgaaaatgc cccttctccc cttcaccgaa acccgcttac ccttgtctga
     6181 cccacagcgc ggtaaagttc gcgataatta tgccctctcg gcagagcgcc gtttgctggt
     6241 taccaccgat cgcctttccg cctttgaccg cattctaggt gccctccctt ataagggcca
     6301 ggttctcaat caactgtctg cctggtggtt tgagcagacc cgctctattg tagataatca
     6361 cgtcctgtcc gtgcctgacc ccaacgcctt gcttgcggtc gctgccgaac ccttcccggt
     6421 tgaggtcatc gtgcgcggct atatcaccgg cgtaaccacg accgccctct ggtaccgcta
     6481 cgccctcggt gagcgtgaga tttatggcta tcgtt
//
LOCUS       DAOPVS010000104         6490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_11828_length_6490_cov_59.170163, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000104 DAOPVS010000000
VERSION     DAOPVS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11828_length_6490_cov_59.170163"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="PKL11_08300"
     tRNA            complement(2..74)
                     /locus_tag="PKL11_08300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Glu,seq:ctc)
     gene            296..1396
                     /locus_tag="PKL11_08305"
     CDS             296..1396
                     /locus_tag="PKL11_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNZ13644.1"
                     /translation="MSIDFIVRLIGMVVLAVVGVYWGTALGRAANVNPDPNTFSVEQY
                     AFTIGLVGALTGLVLTPLLTTRPVRALRSLLTRLSAQSLFSALLGLIAGLIIAALAAF
                     PLSKLPEPIGNVLPFIGVLVFGYIGVSVFVMRQNDIFAVMQSITKSGGQSSADGEKSA
                     EPLADGRTILLDTSVIIDGRIADIAHTGFLPGALLIPRFVLVELQYIADSPDSLRRQR
                     GRRGMEVLSQLQKEPGVPVRISDIDVEGVRDVDDKLVILARQLRCPILTNDYNLNRIA
                     ELQGVTVLNVNDLANAVKSVLLPGEALNVRVIQEGRESNQGVGYMDDGTMVVVESGRD
                     YMNQEILVTVTKVLQTAAGRMIFGRPEDRPER"
     gene            1407..2813
                     /gene="cysS"
                     /locus_tag="PKL11_08310"
     CDS             1407..2813
                     /gene="cysS"
                     /locus_tag="PKL11_08310"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNZ13645.1"
                     /translation="MALRIYNTLTRRKEDFETLEPGRVRMYVCGPTVYNKAHIGHAMS
                     ALVFDIIRRYLEYRGYAVTYAMNFTDVDDKIIARANQMGVDPFGLAEQYITEYQHHLA
                     DLNVLPVTLHPRATQEMDQIISMVQGLIDRGYAYPAGGDVYFRVTRDADYGRLSGRRI
                     EEMQAGARIEVGDLKENPMDFALWKGAKPGEPAWESPWGPGRPGWHIECSAMNLHHLG
                     EQIDIHGGGNDLIFPHHENEIAQTESLTGKQFARYWIHNGMLQLSGEKMSKSLGNLIT
                     IEEFLAHHEGDVFRMVILNSSYRGPLTYTEDVAQQAERGLERLRSALRPALPGSTGAD
                     VPALDVLSAQMQTAQTGFVDAMDDDFNTAGALAVLFELVRVINQTRAAGATDAQLQPA
                     QACLSELLSVLGLHPTATADQSTGQADAFIDLLLELRKELRQQKLWALSDRIRDRLTD
                     LGVTIEDSKEGSSWRWSR"
     gene            2821..3465
                     /locus_tag="PKL11_08315"
     CDS             2821..3465
                     /locus_tag="PKL11_08315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13646.1"
                     /translation="MEDPYPPAGFDDWAASYDDSVADSTGFPFEGYARVLETVFTRAA
                     VQPGMTVLDLGTGTGLLAAPFAAAGCDLWCTDFSAPMLEKARARLPQAHFALHDLRQP
                     WLPEWSQRFDRIVSGYVFHHFEDEQKLALILELVSRHLKPDGRLLIADLGVVEPTDWP
                     AVEQRAGGDWEAELYWTASLMVPAIQALGLRVTYEQISYCAGVYQITMNDRMII"
     gene            3466..4212
                     /gene="rlmB"
                     /locus_tag="PKL11_08320"
     CDS             3466..4212
                     /gene="rlmB"
                     /locus_tag="PKL11_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNZ13647.1"
                     /translation="MKEWITGRNPVYEVLKANRRQFFRLLLATGLEEKARLQEITRLA
                     GERRLKIERVPRQQLDALANGPESHQGVALQASGYMYSDVPAIIAAAEKSGEPLFVLA
                     LDALQNPQNVGTLLRTAGAVGVHGVIIPLAHTATITPAAIHASAGVAEHLRVAQVNLA
                     QALSTFKQAGAWVIGLEGDPDSQPVEQTRLDGPLVLVVGSEGEGMRLLVSRSCDLRIR
                     LPMSPAVESLNAAVAGSVALYLALLARRKA"
     gene            complement(4262..4765)
                     /locus_tag="PKL11_08325"
     CDS             complement(4262..4765)
                     /locus_tag="PKL11_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNZ13648.1"
                     /translation="MENLGQIALQIIILAVMLLGLFSLLTMVVPGLTIIWAAALIYGL
                     VTGFAWGSGIIFAFITLFMIGGNVVDNIMMGAGARQQGASWMAIIVALLAGVIAGIFW
                     TPFGGLAVALAAIFIVEWIRQKDWRKAWDSTREMAMGCGWAGVIRFTIGMVMIGLWWL
                     WAFVINR"
     gene            complement(4796..6073)
                     /gene="serS"
                     /locus_tag="PKL11_08330"
     CDS             complement(4796..6073)
                     /gene="serS"
                     /locus_tag="PKL11_08330"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNZ13649.1"
                     /translation="MLDINLIREQPDVVRQALKIRQMNPGVVDDLQQMDARRRALITE
                     VELLKAQRNAVSKEIGKSKDAAERQAKIEAMRQVGDQIDALDVQVKQVDEELQALVAS
                     IPNLPDPETPYGLSDADNVVLRQVGEIPQYDFTPIPHWDLAPQLGILDLERGVKITGS
                     RFYVLSGAGARLQRALIAWMLDLHIRQGYTEKYTPFMVRAGTLFASGQLPKFVDNLYH
                     DIEEDLWMVPTAEVPLTGLHMEEILDEGSLPLRYTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYIFCRPEESERELEKMLADAEATCAGLGLTYRILRLCSGDLGFNARVTYD
                     LEVWSPGCGEWLEVSSVSNVGDFQARRANVKYRPADGGKTRLVHTLNGSGLGLPRTLI
                     AVMEHYQQADGSIVVPEVLRPWMGGIERIEKPE"
BASE COUNT         1280 a         2026 c         1782 g         1402 t
ORIGIN      
        1 gtgcccccaa ggggactcga acccctgttt tggccttgag agggccgcgt cctgggccgc
       61 tagacgatgg gggcgtgaag cggcaggatt gtatcatgcc gctgtacaga tgtcaacgtc
      121 attgactatt tttaatcacc gtgctatcat ccaatatgta cagtttcatc aaggttgaaa
      181 cacccccgct ggggatgagc gcgtctttca tttattatca ttctttattc tggcgcagtt
      241 tttctcatca ttatccagca cattaacaaa tcataggttt aggaggaatt attcgatgag
      301 cattgatttt atcgtgcgtt taattgggat ggtcgtattg gctgtggttg gcgtctattg
      361 gggcactgcc ctgggacggg cagccaatgt caaccccgac cccaacacct tctcggttga
      421 gcagtatgcc ttcaccattg gattggtcgg cgctctgacg ggcctggttc tcaccccact
      481 gctgaccacg cgcccggtgc gcgccttgcg ttcgctgctc acccgccttt cagcgcaatc
      541 gcttttttcg gccctgctgg gcctcattgc tggtttaatt atcgccgccc tggcggcttt
      601 cccgctttcc aaactgccag agccaatcgg caacgtcttg ccgttcattg gcgtcctggt
      661 ctttggctat attggcgtct cagttttcgt catgcgccag aatgatattt ttgccgttat
      721 gcagtccatt accaaaagcg gtgggcaatc ctctgccgat ggcgaaaaat ctgccgagcc
      781 tctcgccgat ggccgcacca tcctgttgga taccagcgta atcattgatg gccgcattgc
      841 cgatattgcc cacactggct tcctgcccgg tgccctgctc attccccgct ttgtccttgt
      901 tgaactgcag tacatcgccg actcgcccga cagcctccgc cgccagcgcg gccgccgggg
      961 catggaagtg ctttctcaac tccagaagga gcccggcgtt cctgtgcgta tcagtgatat
     1021 tgatgtcgaa ggtgtccgcg atgtggacga caagctcgtc attctggccc gccagctgcg
     1081 ctgtcccatc ctgactaacg actacaatct caaccggatt gccgagctgc agggcgttac
     1141 cgtcctcaac gtcaatgacc tggccaatgc agtcaaaagc gtgctgttgc ccggtgaagc
     1201 cctcaacgtg cgcgtcattc aggaaggccg cgagtccaat cagggcgttg gctatatgga
     1261 tgatggcacc atggtggtcg ttgaaagcgg ccgcgattac atgaatcaag aaattctggt
     1321 caccgtgacc aaagtactgc aaaccgcggc tggccgcatg atttttggcc gccccgaaga
     1381 tcgccccgaa cgctaaggag tttgatatgg ctctgcgaat ttacaacacc ctgactcgcc
     1441 gtaaagaaga ttttgaaacc ctcgaacccg gccgcgtccg catgtatgtc tgcggcccca
     1501 cggtctataa caaggcacat attggtcatg ccatgtcggc cctggtcttt gatatcatcc
     1561 gccgctatct tgaataccgc ggctacgctg tgacctacgc catgaatttt accgatgtag
     1621 atgacaaaat cattgcccgc gccaaccaga tgggcgtaga tccctttggc ctggccgagc
     1681 aatatatcac cgaataccag catcacctgg ctgacctaaa cgtcctgcct gtcaccctgc
     1741 acccccgcgc cacccaggag atggatcaaa ttatcagcat ggtgcagggc ctcattgacc
     1801 gcggctatgc ttatcccgcg ggcggcgatg tctacttccg cgtcacccgt gatgcggatt
     1861 atggtcgcct ctcgggccgc cggattgaag aaatgcaggc cggtgcgcgc atcgaagtgg
     1921 gcgacctcaa agaaaatccg atggactttg ccctctggaa gggcgccaag cccggcgaac
     1981 ccgcctggga aagcccgtgg ggccctggcc gccccggctg gcacattgaa tgctccgcca
     2041 tgaatctgca tcatcttggc gagcagattg atattcacgg cggcggcaat gacctgatct
     2101 tcccccacca cgagaatgag atcgcccaga ccgaaagcct gaccggcaaa caatttgccc
     2161 gttactggat ccacaacggc atgctgcagc tctccggcga aaagatgtcc aaatcgctgg
     2221 gcaacctgat taccattgag gaatttttgg cgcaccatga aggcgatgtc ttccgcatgg
     2281 tcatcctcaa ttccagctat cgcggcccgc tcacctatac tgaagacgtg gcccagcagg
     2341 ctgaacgcgg cctcgagcgc ctgcgctcgg ccctgcgccc ggccctgccc ggctcaaccg
     2401 gcgccgatgt ccctgcgttg gatgttcttt cagcccagat gcagaccgcc cagactggct
     2461 ttgtcgatgc gatggacgat gacttcaata ccgccggagc cctggctgtt ctctttgaac
     2521 tggtccgcgt gatcaaccag acccgcgctg ccggcgccac cgatgcccag cttcagccag
     2581 cccaggcctg cctgagcgaa ctgctgagtg tgctgggttt gcacccgaca gccacggcag
     2641 atcaatcaac agggcaggcc gatgccttta ttgacctgct gctcgaactg cgcaaagaat
     2701 tacgccagca aaaactctgg gcgctctcag accgcatccg tgaccggttg accgacctcg
     2761 gcgtaaccat cgaagacagc aaagaaggct cctcctggcg ttggagccgt tgatcgccgc
     2821 atggaagatc cctatccgcc tgctggcttt gatgattggg ctgcatctta tgatgacagc
     2881 gtagccgaca gcacaggctt cccttttgaa ggctatgccc gcgtccttga gacggtcttt
     2941 acccgcgccg cagtccaacc cggcatgacg gtgctggacc tcggcacagg cacggggctg
     3001 ctggccgcgc cctttgcggc ggctggctgt gacctctggt gtaccgactt ttcagccccc
     3061 atgttagaga aggcccgtgc ccgcctgccg caggcgcatt tcgctctcca cgacctgcgc
     3121 cagccctggc tgcccgaatg gtcgcagcgt tttgaccgca ttgtctcggg ctatgtcttc
     3181 catcattttg aagacgagca gaagcttgcc ctgatcttgg aactggtttc ccggcatcta
     3241 aaacctgatg gccgcctgct gattgcagac ctcggcgtag tcgagccaac cgattggccg
     3301 gctgtagaac agcgcgccgg cggcgattgg gaagccgaac tgtactggac agccagcctg
     3361 atggtcccgg caatccaggc cctggggctg cgggtgacct atgagcaaat ttcctattgc
     3421 gcgggtgtgt atcaaattac catgaacgat cggatgataa tttaaatgaa ggaatggatt
     3481 actgggcgta acccggttta tgaggttctg aaggccaacc ggcggcagtt ttttcgcctg
     3541 ctgttggcca ccggcctgga agagaaagcc cgcctgcaag agatcacccg gctggctggt
     3601 gagcgccgcc tgaagattga gcgcgtcccc cgccagcagt tggatgccct ggcaaacggc
     3661 cctgagagtc atcagggtgt ggcgctgcag gccagcggct acatgtacag cgacgtgccc
     3721 gcaatcatcg ccgctgccga aaaatcgggc gagccgctat ttgtgctggc cctcgacgcg
     3781 ctgcaaaatc cccagaacgt aggcacgctg ctccgcacgg ctggcgcggt gggagtgcat
     3841 ggcgtcatca ttcccctggc acacaccgcc accattaccc ccgcggcaat ccacgcctct
     3901 gccggggtgg ctgaacacct gcgcgttgcc caggttaacc tggcgcaggc gctgtccacc
     3961 ttcaaacagg ctggcgcctg ggtcatcggc ctggagggcg atcctgattc gcagccggtg
     4021 gaacagaccc gtctcgatgg ccccctggtg ctggtggttg gcagcgaggg cgaaggcatg
     4081 cgcctgctgg tctcgcgctc ctgtgatttg cgcatccgcc tgcccatgtc cccggcagtc
     4141 gaatcgctca atgccgcggt agccggttct gtggcgcttt atctggccct gttggctcgc
     4201 cgcaaggcct gagtcaggat gaatcaaaaa agcggcaggc tttttgcctg ccgcttttcg
     4261 tttagcggtt gataacaaac gcccacagcc accacaggcc aatcatcacc atgcctatgg
     4321 tgaaccggat gaccccggcc cagccgcagc ccatcgccat ctcacgggtt gaatcccagg
     4381 ccttgcgcca gtccttttgt cgaatccatt ccacaatgaa aatggcagcc agcgccacag
     4441 ccagcccgcc aaatggcgtc cagaagatac ccgcaatcac gccagccagc agggcaacaa
     4501 taattgccat ccagcttgcg ccttgctggc gcgccccagc gcccatcatg atgttgtcca
     4561 ccacgttgcc gccgatcata aacagggtga tgaaagcgaa gatgatcccg ctgccccagg
     4621 cgaacccggt gaccagccca taaatcaacg ccgccgccca gatgatcgtc agcccgggca
     4681 caaccattgt caggaggctg aagagcccca gcagcataac cgccaggata atgatctgca
     4741 gtgcaatttg gccgaggttt tccatggctt cagtttacca taaaagcgct ttgggctatt
     4801 ccggtttttc gatccgctcg atgccgccca tccaggggcg cagcacttcg ggcaccacaa
     4861 ttgagccgtc agcctgttga taatgctcca tcacggcgat caatgtccgc ggcagcccca
     4921 gccccgatcc attgagtgtg tgcaccagcc gggttttgcc gccatctgca gggcgatatt
     4981 taacattggc gcgccgcgcc tgaaaatcgc ccacatttga gaccgacgaa acttccagcc
     5041 attcaccgca tcccggcgac cagacttcca gatcataggt cacccgggca ttaaagccca
     5101 ggtcaccgct gcacagccgc aggatccggt aggtcaatcc cagcccggcg caggtagcct
     5161 cggcgtctgc cagcatcttc tccaactcgc gctccgattc ttctggccgg cagaagatgt
     5221 acatctccac tttgtcgaac tggtgcccgc gcttgatgcc gcgcacgtcg cgcccggcgc
     5281 tcatcttctc ccgccggaag cagggagtat aagccgtata acgcagcggc aggctgccct
     5341 catccagaat ttcttccatg tgcagcccgg tcagcggcac ttcggcggtt ggcaccatcc
     5401 acaggtcttc ttcaatgtcg tgatacaggt tgtccacaaa cttgggcagc tggcccgagg
     5461 caaacagggt acccgcgcgc accataaagg gcgtatattt ctcggtatat ccctggcgga
     5521 tgtgcaggtc cagcatccag gcgatcaacg cccgctgcag ccgggcccct gcgccgctca
     5581 acacataaaa gcgcgagccg gtgatcttga ccccgcgttc caggtccaga atgcccagtt
     5641 ggggcgccag gtcccagtgt gggatgggag tgaaatcata ctggggaatc tcacccacct
     5701 gccgcagcac cacgttgtca gcgtcgctca gaccgtaagg tgtctcaggg tccggcaggt
     5761 tggggatgga ggccaccagc gcttgcagtt cctcgtccac ctgcttgacc tgtacatcca
     5821 acgcgtcgat ctggtcgcca acctgacgca tagcctcaat cttggcctgg cgctcagccg
     5881 catctttgga ctttccaatt tcctttgaaa ccgcgttgcg ctgtgccttc agcaattcca
     5941 cttcggtaat cagggcgcgc cggcgcgcgt ccatctgctg cagatcatcc accacgccag
     6001 ggttcatctg gcggatcttc agcgcctgac gcaccacgtc aggctgttca cgaatgagat
     6061 tgatgtcgag cataaaagcc tcctgagcag aaatcaaaag cccccacact cgtgcaggac
     6121 gaggggggga ctgctcgtgg tgccacctgc ttttgccgcc cgtgcggcct cattggggga
     6181 taacggtttg cgccggcctg cttcggacct tggccccggc gcaggcaacc cgccgtttgc
     6241 acagcgaccg gggtggatgg ttttggcgct cctgtcagct ttcaccagcc gctgactctc
     6301 tgaaaggtgg accagaacct gtccccgggt gggttgttga atatgtcttg attatagcgg
     6361 agtctcacga cttgtcaagc ggaggtcatg caacttttgt aaagcaggtc aattgtaatg
     6421 gcttgagctt tgaaaataaa ttcttccaat gctctgacgt gcaggttgat tagtccgctg
     6481 atcgagtagt
//
LOCUS       DAOPVS010000105         6426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_12002_length_6426_cov_54.361482, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000105 DAOPVS010000000
VERSION     DAOPVS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12002_length_6426_cov_54.361482"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..1287
                     /locus_tag="PKL11_08335"
     CDS             460..1287
                     /locus_tag="PKL11_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ13650.1"
                     /translation="MTHIFDQFKLSGQAAVVTGGAGLLGKGFCRSLAQAGAGVIVADL
                     NTAAAEAVAAELQSEGLAALGVGVDVTNPDSVRAMVEACTNTYDRLDILVCSAAMDPK
                     FDQDNVGQQGANAFENYPLTAWRQALDVNLTGMFLCAQAAVQPMIAQDHGVIINICST
                     YGLVGPDQRLYRRPGQETQNYKPVFYSVTKAGVLGLTRYLATYYAGRNIRANALTPGG
                     VYNNHDEVFTQQYSARTVLGRMARREEMNGAMLFLASDASSYMTGANLVVDGGWTAW"
     gene            1281..2513
                     /locus_tag="PKL11_08340"
     CDS             1281..2513
                     /locus_tag="PKL11_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HNZ13651.1"
                     /translation="MVARPEVLAIIPARGGSKGIPRKNIRNFAGYPLIAYSIAAGLQA
                     ETVTRVIISTDDAEIAAVAQQHGAEAPFLRPAEFAQDATTDLPVFEHALRWLAEHENY
                     HPDLVIQLRPTSPIRPPALVDETVRLLLAHPEADSVRGVVPAGQNPHKMWRLTGPESP
                     MVPLLEVPGIAEPYNAPRQILPPVYWQTGHIDVIRPAAILEQGSMSGRTILPILIDPN
                     YTVDIDTPRDWQRYEWLVWYGGLEMVTPHRPRRPLPPRVSLIIMDFDGVLTDNRVWTD
                     QAGHESVAAFRGDSMGLNELRHRLGTQALVLSREANPVVSARCKKMNIPCIQSALDKG
                     STLPALLAEYHVSPAEVVYIGNDINDLPCFPLVGCAVVPADAEPAVLREADLVLTRTG
                     GHGAVRELCDLILQNNQE"
     gene            2519..3400
                     /locus_tag="PKL11_08345"
     CDS             2519..3400
                     /locus_tag="PKL11_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNZ13652.1"
                     /translation="MTRAVKIGDRMIGDGYPTYIIAEIGINHNGDIEIAKQLIDAAKH
                     AGVDAVKFQKRTPELCVPPEQRNQMRETPWGYITYLEYRYKTEFGAEEYAEIDRYCQQ
                     VGIDWFASVWDEPSVDFLEQFNPLCYKVPSASLTDHGLLRHLKNTGRPIILSTGMSTM
                     EQIRTAVPLLDMDKLVICHTTSAYPCEPEELNLRMVQTLRDIYPCPIGYSGHEVGLVP
                     SAVAVALGACVVERHITLDRAMWGSDQAASVEPGGFERLVKYIRVTEQSVGDGVKRVY
                     ASELPSQRKLRRVNGES"
     gene            3582..3962
                     /locus_tag="PKL11_08350"
     CDS             3582..3962
                     /locus_tag="PKL11_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13653.1"
                     /translation="MKKAGKQYMIRFSISMWIYALVLVLAVLFVNANRESALRFPVML
                     LPAVPIFFALLAFVNYFQTMDELQKRIQLYALALSVGGTAMIAMTYGLLELVGAPHLS
                     WVWIFPLIVGLWGLGFYIASRRFS"
     gene            3959..4168
                     /locus_tag="PKL11_08355"
     CDS             3959..4168
                     /locus_tag="PKL11_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ13654.1"
                     /translation="MKNQLKVLRAQRDWSQADLADRLDVSRQTVNAIETGKYDPSLPL
                     AFKIARLFGLTIEQVFEEEPEEGRT"
     gene            complement(4242..5537)
                     /locus_tag="PKL11_08360"
     CDS             complement(4242..5537)
                     /locus_tag="PKL11_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNZ13655.1"
                     /translation="MKALLLNGSDANDLTGQRLQQALEAALQARGWQVEPVLLREKKI
                     APCAGDFFCWVRTPGVCNFDDDNRQIAASIVNSDLVIDLTPVTFGGYSALLKRAVDHL
                     IQNISPYFTRLAGETHHRRRYARYPDFLLAGWQAAPDLQAEGIFRHLAHRNTLNFYAR
                     RSVCGVALSTQSDAELRAQADAWLQALEAGAQIPATALPQPPLAPAAAVPPRRAVLLV
                     GSPRGRGSSSHALGSYLMEQLAARSVQTDTILIYPALNQPEKMQALLQTLAEADLAVL
                     AFPLYVDSLPAPVITLLEQVAARQPKPLRFAAITNCGYPESQHGANALAVCGQFAQQV
                     GFGWAGGLTLGAGPGLVHGTPLNELDGRAIPVKLALDQAAEALAKGEAIPPTAQAIWN
                     RPVIPAGLYRLMGNLGWKPQASKYSAVKHLRRKPYTETQ"
     gene            complement(5541..6236)
                     /locus_tag="PKL11_08365"
     CDS             complement(5541..6236)
                     /locus_tag="PKL11_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ13656.1"
                     /translation="MSPRLPRTAEKPNLPQAIKAAAWQQIAESGAPALSLRAIARRLG
                     ITAPAIYNYFPDRDALVTALVIEAFTSFGDSQLAARDAIPPADLQGRFMATGWAYRQW
                     ALDYPQRYQLIFGTPLPGYMPPSEQTFPAGARALSALASVVEALRAAGRLHTGDFPQP
                     APGGCLDLFELWKKFGGEVDLLSLSVAVLIWARVHGLVSLEIGGSLPPFEPTGEALYR
                     FELNAIWRQFFQE"
BASE COUNT         1237 a         1960 c         2017 g         1212 t
ORIGIN      
        1 ggcaaggcca gtccgcccct gcaaatcctt acagggtgct gcgcgaccct cgccgcttcg
       61 gcgctccggc gggagtgcag ggtgctgcgc gaccccgaaa gagccgccgg ggattgaaat
      121 ccccggctaa tgaagaacaa gtcggctgcg ccgactccgt ctggatgagt ccgctttagc
      181 ggacttggtt ttagccaggc ggcgacatgt gccccgcatg gggtatttta atcgccgccg
      241 gtggtgagca gggctgcgcg atcctcgccg tttcggcgct ccggcgggag tgcagggccg
      301 ggcggacaca caggtccgcc cctacaaatc cttgcggggt gctgcgcgac ccctttgcag
      361 aaaaaccctt gtccgccgac caacaatccc aaaacctaaa ccctgattcg cggtacaata
      421 cacccaaaat catcaacttt ggatgtgagg gatacaatcg tgactcatat ttttgatcaa
      481 ttcaaactca gcgggcaggc ggcagtggtg acgggcggcg ccgggctgct gggcaagggc
      541 ttctgccgct cgctggcgca ggccggcgcc ggtgtgattg tggccgacct caacacggcc
      601 gcggctgaag ccgtggctgc cgaactgcaa tctgagggcc tggcggcgct tggcgtgggc
      661 gtggatgtga ccaaccccga ctcggtgcgc gccatggtgg aagcctgcac caacacctac
      721 gaccgtctgg atatcctggt ctgctctgcc gccatggacc ccaaatttga tcaggacaat
      781 gtggggcagc agggcgccaa cgcctttgaa aattatcccc tcacggcctg gcggcaagcg
      841 ctggatgtga acctgacggg catgttcctg tgcgcccagg cggccgtcca gccgatgatt
      901 gcccaggacc acggcgtgat catcaacatt tgctccacgt acgggcttgt gggccccgac
      961 cagcgcctct accgccgccc cgggcaggag acgcaaaatt acaagccggt tttctattcg
     1021 gtgaccaagg ccggtgtgct gggcctgaca cgttacctgg cgacctatta cgccggccgc
     1081 aacatccgcg ccaatgcgct gacccccggc ggggtgtata acaatcacga tgaagtcttc
     1141 acccagcagt attcggcccg cacggtgctg ggccgcatgg cccgccgtga ggaaatgaac
     1201 ggcgccatgc tcttcctggc ctccgatgcg tcctcgtaca tgaccggcgc caacctggtg
     1261 gtagatgggg gctggacggc atggtagccc gccccgaagt gctggccatc atcccggcgc
     1321 gcggcggctc caagggcatc ccgcgcaaga acatccgcaa ttttgccggc tacccgctga
     1381 ttgcctacag cattgccgcg ggcctgcagg ccgaaacagt cacacgggtc atcatctcta
     1441 ccgatgacgc cgagattgcc gccgtggcgc agcagcacgg cgccgaggcg cccttcctgc
     1501 gcccggccga atttgcccag gacgccacca ccgacctgcc cgtgtttgaa cacgcgctgc
     1561 gctggctggc tgaacacgaa aactatcacc ccgacctggt gattcaactg cgccccacct
     1621 cgcccatccg cccgcccgcg ctggtggatg agaccgtgcg gctgctgctg gcccaccccg
     1681 aggccgactc ggtgcgcggc gtggtgccgg ccgggcaaaa cccgcataag atgtggcggc
     1741 tcaccggccc cgaaagccca atggtgccgc tgctggaagt gcccggcata gccgagccct
     1801 acaacgcccc ccgccagatc ctgccgcccg tgtactggca gaccgggcat attgacgtga
     1861 tccgcccggc ggcaattttg gagcagggct cgatgagcgg gcgcaccatt ctgcccattc
     1921 tgattgaccc caactacacc gtggatattg acaccccgcg cgactggcag cgctacgagt
     1981 ggctggtctg gtacggcggg ctggagatgg tcacccccca ccgaccccgc cgcccgctgc
     2041 ctcccagggt ctcgctgatc atcatggatt ttgacggcgt cttgaccgac aaccgtgtct
     2101 ggaccgacca ggccgggcac gagagcgtgg cggccttccg cggcgacagc atgggcctca
     2161 acgagctgcg ccaccggctg ggcacccagg cgctggtgct ctcgcgcgag gccaacccgg
     2221 tggtctcagc gcgctgcaag aagatgaaca tcccctgcat ccagagcgcg ctggacaaag
     2281 gctcaaccct gcccgccctg ctggctgaat accacgtctc gcccgctgag gtggtctata
     2341 ttggcaacga cattaacgat ttaccgtgct tcccactggt gggctgcgcg gtggtccccg
     2401 cagatgccga acctgccgtg ctccgtgaag ccgacctggt gctgacccgc acaggtggtc
     2461 acggcgcggt gcgcgaactc tgcgatctta ttttgcaaaa caatcaggag taacttttat
     2521 gactcgtgct gtaaaaattg gcgaccggat gatcggtgac ggctacccga cctatatcat
     2581 tgccgaaatt ggcatcaatc acaacggcga tattgaaatt gccaagcagt tgattgatgc
     2641 cgccaaacac gccggcgtgg atgcggtcaa attccagaag cgcacacccg agctgtgcgt
     2701 gccccccgaa cagcgcaatc aaatgcgcga gacgccctgg ggctacatca cctatctgga
     2761 ataccgctac aagaccgagt ttggcgctga agaatacgcc gaaattgacc gctactgcca
     2821 gcaggtgggc attgactggt ttgcctcggt gtgggatgag ccctcggtgg attttctgga
     2881 gcagttcaac ccgctgtgct acaaggtccc ctcggcctcg ctgaccgatc atgggctgct
     2941 gcggcacctc aagaataccg ggcgccccat catcctctcg acgggcatgt ccaccatgga
     3001 acagatccgc acggcagtgc ccctgctgga tatggacaag ctggtgatct gccataccac
     3061 cagcgcctat ccctgcgagc ccgaagagct gaacctgcgc atggtgcaga ccctgcgcga
     3121 catctatccc tgcccgattg gttactccgg gcacgaggtg gggctggtgc cttccgccgt
     3181 ggctgtggcg ctgggcgcct gcgtggtaga gcggcacatc accctcgacc gggccatgtg
     3241 gggcagcgac caggccgcct cggtggagcc gggcgggttt gagcgcctgg tcaaatacat
     3301 ccgcgtgacc gagcagtctg tgggcgatgg cgtcaagcgc gtctatgcca gcgaactgcc
     3361 ctcacagcgc aagctgcgcc gggtcaacgg cgagtcgtag cgcgggcctg gggttagaaa
     3421 ttcgggcgtg cggctttagg ccgcacgcct gtttttttat tttgttgggt cgaaagagga
     3481 aaaaagacaa gtttcatttc caaaatgtaa attaagcttg acaaaacgta aagcttgctg
     3541 tatactaatg acattcagaa aacagaatag gagaacagga aatgaaaaaa gctggaaaac
     3601 aatacatgat acgattttca atatcgatgt ggatatatgc tcttgtgctg gtcttggcgg
     3661 tcttatttgt gaatgcaaac cgcgagtcag ccttgcggtt cccggtgatg ctgctcccgg
     3721 cagtacccat tttctttgca cttttggctt ttgtgaacta ctttcaaacc atggatgaac
     3781 tgcaaaagcg catccagttg tacgcgctgg ctttgtctgt gggggggacg gccatgattg
     3841 caatgaccta tgggctgctt gaattggtcg gcgcgccgca tctttcgtgg gtgtggatct
     3901 ttccgctcat tgtggggttg tggggcctcg gcttttatat cgcctcacga aggttctcat
     3961 gaagaaccag cttaaagtgc tgcgcgcaca gcgtgactgg tcacaagctg acctggcgga
     4021 ccgcctggac gtttcccgcc agacggtcaa cgcgatcgaa acgggcaaat acgatcccag
     4081 tctgccgctg gctttcaaga ttgcacgcct gtttgggcta acaatcgagc aagtctttga
     4141 agaggagccc gaagagggaa ggacttagat ccaatcttaa taatgatgtg gggtacaccc
     4201 cagtaaatat ttttcagata gctactaaaa gggaacccgg gttactgcgt ctcggtatag
     4261 ggtttgcggc ggaggtgttt gacggcgctg tatttgctgg cctgcggctt ccagcccagg
     4321 ttgcccatca agcggtagag cccggcggga atcacggggc ggttccagat ggcctgcgcc
     4381 gtcggcggga tggcttcgcc ttttgccagg gcttccgccg cctggtccag cgccagtttg
     4441 accgggatgg cgcggccgtc cagctcgttg agcggagtgc cgtgcaccag gcccggcccg
     4501 gctcccagtg tcagcccgcc ggcccagcca aagcccacct gctgcgcaaa ctgaccgcac
     4561 actgccagcg cattggcgcc atgctgcgat tcggggtagc cgcagttggt gatggcggca
     4621 aaacgcagcg gtttgggctg ccgcgcggct acctgctcca gcagggtgat caccggcgcg
     4681 ggcaggctgt ccacatagag ggggaaggcc agcaccgcca ggtcagcctc ggccagggtt
     4741 tgaagcaggg cctgcatttt ctcgggctgg ttgagcgccg gataaatcag gatggtgtct
     4801 gtctgcacgc tgcgggcggc aagttgttcc ataaggtagc tgcccagggc gtgagagctg
     4861 ctgccgcggc cccgcgggct gcccaccaaa agcacagccc ggcgcggcgg cacggcggct
     4921 gccggagcca ggggcggctg gggcagcgcc gttgcgggga tctgcgcgcc agcttccagc
     4981 gcttgcagcc aggcatcggc ctgggcgcgc agttcagcat ccgactgggt ggacaacgcc
     5041 acaccgcaaa ccgagcgccg ggcgtaaaag ttgagcgtgt tgcggtgcgc caggtgccgg
     5101 aagatgccct ctgcctgaag gtcgggggca gcctgccagc cggcgagcag aaaatcgggg
     5161 tagcgagcat agcggcgccg gtggtgggtt tcacctgcca ggcgggtgaa atagggcgaa
     5221 atgttttgaa tcagatgatc caccgcccgc ttgagcaggg ccgaataccc gccaaaggtg
     5281 accggggtca ggtctatgac caggtcgctg ttgacgattg acgccgcaat ctggcggtta
     5341 tcatcatcaa aattacagac gcccggggtg cgcacccagc agaaaaaatc accggcgcag
     5401 ggcgcaatct tcttttcgcg cagcaaaacc ggctccacct gccagccgcg ggcctgcagg
     5461 gcagcctcca gcgcctgctg caggcgctgc ccggtgagat cattggcatc cgaaccattg
     5521 agcagaaggg ctttcatcat ttactcctga aaaaactgac gccagatggc gttcaactca
     5581 aaccgataga gggcttcgcc ggtaggctca aacggcggca ggctgccgcc aatctcgagc
     5641 gagaccaggc cgtgcacgcg ggcccagatg agcaccgcca ccgacagcga aagcaggtcc
     5701 acctcgccgc cgaatttttt ccacagttca aacaggtcca ggcagccgcc cggggctggc
     5761 tgtgggaagt cacccgtgtg cagccgcccg gcggcgcgca gcgcctccac cacgcttgcc
     5821 agcgcgctga gggcccgcgc ccccgccgga aaggtctgct ctgagggcgg catatacccc
     5881 ggcagcggcg tgccaaaaat cagttggtag cgctgcgggt aatccagcgc ccactggcgg
     5941 taagcccagc cggttgccat gaaccgcccc tgcagatcag cgggtgggat ggcatcccgc
     6001 gcggcgagct gtgaatcgcc aaacgaggta aaggcctcaa tcaccagggc agtcaccagg
     6061 gcgtcgcggt cggggaaata gttgtagatg gccggggcag tgatgcccag gcggcgggca
     6121 atggcccgca gactgagtgc cggtgcgccc gattcggcaa tttgctgcca ggcggcggct
     6181 ttgatggcct ggggcaggtt gggtttttcg gcggtgcggg gcaggcgagg ggacatgggc
     6241 ctcctgaaat ctaaacgctg tatatttaca gtgtaaatat acagcgttta tttgaggttg
     6301 tcaaggggtt agatttggtg ctggctgccc cgggtttccg ggcaatgctt gtcgctttta
     6361 tggggttttg aacgccgcgt cttttcagca gtgctataat taagcgtaca ggagccgccc
     6421 atggtt
//
LOCUS       DAOPVS010000106         6417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_12017_length_6417_cov_54.780572, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000106 DAOPVS010000000
VERSION     DAOPVS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12017_length_6417_cov_54.780572"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..724
                     /locus_tag="PKL11_08370"
     CDS             50..724
                     /locus_tag="PKL11_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ13657.1"
                     /translation="MTTIIPLKLSISNAFLLRGPKDILVDAGNPLDLPLLTRRLHQWG
                     VDIGALSLIILTHVHFDHCGTLAALKKQTKCPVMVHQLEKEYLELGRNAPIIPIHPLG
                     KAFMPFMDARFSPAAPDLLVDDALDLSTYGVSAQVIFTPGHTPGSVSILTDDRQAIVG
                     DLCGGGWPLGQFQPHKPRYHYWASNPNDIQTSLKRILSFQPVKIHVGHGGPLDGRAAA
                     EFFLVA"
     gene            complement(835..1692)
                     /locus_tag="PKL11_08375"
     CDS             complement(835..1692)
                     /locus_tag="PKL11_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13658.1"
                     /translation="MNLQTGETKQTLEAKAYVMVQTIRSIESAFYMSLWVLSKSASAA
                     ADGLDEANPIHLFAEMCQQAAQDWEVDVQKLRSACLERGTDNAVALEGIVSAIVDWLA
                     RLESLADKLSAVSAERQEDTATLKSLQNQLNHLLARRECLRQRDFSWLAGLPRTTFVN
                     VDELQCTFSDHFKPLGQPVMIPCEDIIARRSGKMQVNPVTQVTYSFGWYRDFECLEFQ
                     IKVVTYAGARPVITNWRLLSTLQTIKLAPYVCEYCGDAVEEQYWACSECGYDILYHAT
                     RPETAPPAG"
     gene            2056..2850
                     /locus_tag="PKL11_08380"
     CDS             2056..2850
                     /locus_tag="PKL11_08380"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNZ13659.1"
                     /translation="MKVYISADIEGITGVTHWDETDQGKEGYEVAREQMTAEVAAACE
                     GALQAGATEVWVKDAHDSARNLIASRLPRQTRLIRGWSGHPFVMMQELDSSFHALVLV
                     GYHSRMGSGANPLSHTMSGHITWLKINGLEASEFSLCAYTAALVKVPLVFVSGDKGLC
                     EDAVSLNSHITTVAVKEGVGDSTINIHPDLAVEQIRSQVARAVAEASKCLLPLPNHFT
                     AEVRFRRPERAYQFGFFPGVRQTDATTVQFDHADFFEVMRFLLFAL"
     gene            2868..4793
                     /locus_tag="PKL11_08385"
     CDS             2868..4793
                     /locus_tag="PKL11_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNZ13660.1"
                     /translation="MDASAEISRLIEESRALERGGRIPAAIHRAVQARQLAQAGADTE
                     SEAAALNALAYAHIRLGHYPQAVAFSQAALALAGPESRARAEALLNINICASETNDLD
                     TGEKYLLQAIDLARQTGDQRILARGLHSISCGIYMPRGQFALSLAADEEASNIIRSSG
                     MMELLWGPILTMSWVYWLTGQYRLTAARLDELRANLLPNSLADGYWHYIHGNLAIENG
                     DYETARSMLQQTRTIAESNGITENLFLAHLGMSRLSRLLNDAPAALGWADEAYHLMER
                     TGYQHLQAQALIERSRAAWALGDLPAAESDLRSAINLLTPQRLAFDLAIATLLLAALL
                     NQQNHPQAPALWQAAAARLTQGGFAFLVDRERSLAFPLIVQGLKSPDKSVVIASNSLL
                     EHLHRVPPPPLKITTLGGWCLKAGGRSIDPQFLRQRRAGELLGFLLISPGRRLPIDQI
                     IDSFWPEKEFNAAQALLHQATSTLRRTLEPDLPEKFPSRYLRVDDGSIALALPPDSQV
                     DYETFMSHIRCADWDAALACCPGEFLPEYRYADWAEAHRRWLAQEYQRALLAKARVWL
                     SEKQFLPTLDACRKLLAAEPWQEQAVLLGMQACLGLGDTAGALRLYLTLEKTLQTDLG
                     VEPQPELQTLYRSLLKR"
     gene            4953..6275
                     /locus_tag="PKL11_08390"
     CDS             4953..6275
                     /locus_tag="PKL11_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13661.1"
                     /translation="MLKSQNSRPVLYLTALLALVISLCLNLAPVRASSFSQNALSGNP
                     NAFTWQKVIAPDGTSKDGFGHSAAISDDGSTAIVGSYYIEFGITYPIGPGGVYIFTND
                     AGTWVLQQKLPTPDMPDSDLFGFSVALSADGNTALVGAQGGTGPVDTQVPGTAYIFTR
                     SEGVWSQQAMLTASDRQVGDHFGVSVALSDSGDTALIGAYVDDVGANTNQGSAYVYQR
                     SGNAWTEQAHLTSTDAAAGDYFGRSVALSADGSMALIGADMKTIGANTAQGAAYIFTR
                     ASTTWSQQTRLTDINGGGYEWFGVGAALSADGQTALVGADGTGAFVFTASGGIWTQQA
                     LLTDGSAYLGWGNSIALSADGNTALVEDGFTFTRHSGSWIAGERLLADDSPSGFGSAV
                     ALSGDASLALIGASYATIGANQSQGAAYFFTKTSSGPQTSIYLPLIVR"
BASE COUNT         1309 a         1953 c         1703 g         1452 t
ORIGIN      
        1 tacaataatt tgaacacccg cttgatcatc gtctctttga ggagccctca tgaccaccat
       61 catccctttg aagctctcaa tatccaatgc cttcctgctg cgcggcccaa aagacattct
      121 tgtggacgcc ggcaacccac tcgatttacc cctactcacc cgccggttac atcaatgggg
      181 cgttgatatc ggcgccctgt ccctgatcat actcacccac gtccactttg accattgtgg
      241 aacgctggcg gccctgaaaa aacagaccaa atgcccggtc atggttcatc agctggaaaa
      301 agagtatctg gagttaggtc gtaatgctcc tattatcccc atccacccgc ttggcaaagc
      361 cttcatgcct tttatggatg ctcggttttc cccagccgcg cccgatctgc tggtcgatga
      421 tgccctggat ttatcgacct atggcgtgag cgcccaggtg atctttaccc ccggccatac
      481 gcctggctct gtttcgatat taactgatga tcgtcaggcc atcgttggtg acctttgcgg
      541 cggcggatgg cccttggggc agttccagcc gcataaaccg cgatatcact actgggcctc
      601 caacccgaat gacatccaaa ccagcctcaa acgcatcctg tctttccagc cggtcaaaat
      661 tcacgtcggc catggcgggc cgctggatgg ccgcgccgct gccgaatttt tcctggttgc
      721 ctgaaaaccg cgtggcttat cggattttgg tttcctggat ccattttaag ttcaggcgta
      781 aggtctgagg cttcctgaat aggcattgtg ctgttataag aattcgctat cacgttatcc
      841 agcaggggga gcagtctctg gacgggtagc gtgataaaga atatcatagc cacattcact
      901 acaagcccag tattgctctt caactgcatc gccgcagtat tcgcagacgt aaggcgccag
      961 tttgattgtc tgcagcgttg aaagcagccg ccagtttgtg atgactggac gcgccccggc
     1021 gtaggtgaca acctttattt ggaactcgag acattcaaaa tcccgatacc atccaaagga
     1081 gtacgtcacc tgtgtgactg ggttaacttg catctttcca gaacgccggg caataatatc
     1141 ttcgcagggg atcatgaccg gctgccccag tggcttgaag tgatccgaaa aagtgcattg
     1201 taattcatcc acattcacaa aggttgtccg gggtaacccg gccagccagg aaaaatcacg
     1261 ctgtcttagg cactccctcc gggctaatag atggttgagt tgattctgca atgatttcag
     1321 ggtagccgta tcttcctgac gctcagcact tacggcagag agcttgtctg ctaaactttc
     1381 aaggcgcgcc agccaatcca caatcgcact cacaatacct tccaatgcaa ctgcattatc
     1441 tgttcctctt tccaggcagg cgctgcgcag tttctgaaca tcaacctccc agtcttgcgc
     1501 agcttgctgg cacatttcag caaaaaggtg gattgggttc gcctcatcca gaccatcagc
     1561 cgcggcactg gcagatttcg ataaaaccca cagcgacatg tagaaagcag actcaatcga
     1621 ccggatggtc tgtaccatga catacgcttt ggcttcgagc gtttgcttgg tttcaccggt
     1681 ttgcaaattc aatttactga gcagctcttg ccattcatcc gggccccccc gaacgttaat
     1741 gatgggcaga tagggttcgt tgttggcaga atctggcgct tttttcttcc cctgattggc
     1801 agattgggtc ggcgggtgct tccgccccag ccggatgagt ataaccagaa ttagccctaa
     1861 aaaaccgagg ccaggcaaac cacacaataa agcaaagaga agaaaagtct cagttagcat
     1921 attcaagtgg aatcaagtaa cctcaaagag atgattggca taaacccaag tgggcgagta
     1981 tcccggagtt gtcatataat caaggttgcc gccctccata attataacta aaacccggag
     2041 ggccaggagc cacccatgaa agtctatatc agcgccgaca ttgaaggcat caccggagtc
     2101 actcattggg acgagaccga tcaaggcaaa gagggttacg aggttgcccg tgagcaaatg
     2161 accgccgaag tcgcagccgc ctgcgaaggc gccctccagg ctggtgcaac cgaagtctgg
     2221 gtcaaagacg cccacgacag cgcccgcaac ctcatcgcct cccgcctgcc ccgccagacc
     2281 cgcctgatcc gtggttggag cggtcacccg tttgtgatga tgcaagagct ggattccagc
     2341 ttccacgccc tggttctggt cggctaccat tcccgcatgg gctcgggcgc caaccctctc
     2401 tcgcacacca tgtctggcca catcacctgg ttgaagatta acggccttga agcctcggaa
     2461 ttttccctgt gtgcctacac cgctgccctg gtcaaagtac cgctggtctt tgtctcgggt
     2521 gataaaggcc tgtgtgaaga tgccgtttca ctcaactccc acatcaccac cgttgccgtt
     2581 aaagaaggcg ttggcgattc caccatcaat attcaccctg accttgccgt ggaacaaatc
     2641 cgctcccagg tggctcgggc tgtggcagaa gcgtcgaagt gcctgctgcc cctccccaac
     2701 cacttcactg cagaagttcg cttccgccgc cctgagcgcg cctatcagtt cggcttcttc
     2761 cctggcgtcc ggcaaaccga tgccaccacc gttcagtttg accatgccga tttctttgaa
     2821 gtcatgcgct ttctcctgtt tgcgctgtaa gacttttggg ccccgcagtg gatgcctcag
     2881 ccgaaatttc ccgcctgatt gaagaaagcc gtgccctgga aaggggcggg cgtatcccgg
     2941 cggccatcca tcgggcggtg caggcgcgcc agttggccca ggctggtgcc gataccgaaa
     3001 gcgaggccgc cgcgctgaat gcgctagctt atgcgcacat ccgtcttggg cattaccccc
     3061 aggccgtggc attcagccag gcagccctgg cactggccgg ccccgaaagc cgggcgcggg
     3121 ctgaggcttt attaaacatc aacatctgtg ccagcgaaac caacgacctc gacacgggtg
     3181 aaaaatacct gctgcaggcc attgatctgg ctcgccagac cggtgatcaa cgcatcctgg
     3241 cgcgtggcct gcactcgatc tcctgcggta tctacatgcc acgcgggcag tttgccctgt
     3301 cgctggccgc cgatgaagag gcaagcaaca tcatccgttc cagcggcatg atggaactgt
     3361 tgtgggggcc catcctgacc atgagttggg tctactggct gaccggccag tatcggttga
     3421 ccgctgcccg cctggatgag ctgcgggcga acctgctccc caattctctg gccgatgggt
     3481 actggcacta catccatggc aaccttgcca tcgaaaatgg ggattatgaa accgcccgga
     3541 gcatgctgca gcaaacccgt accatcgctg agagcaacgg catcaccgag aatctttttc
     3601 tggcgcacct ggggatgagc cgcttgagcc ggctgttgaa cgacgccccc gcagccctgg
     3661 gctgggcgga tgaggcctat cacctgatgg aacgcacggg ctaccagcac ctgcaagccc
     3721 aggcgctcat tgagcgcagc cgtgccgcct gggcattagg agacctgccc gcagccgaat
     3781 ccgatctgcg ttcagccatc aacctgctca ccccccagcg cttggccttc gatctcgcga
     3841 ttgccaccct gctcctggcc gccttgctga atcagcaaaa tcatccccag gcccccgccc
     3901 tctggcaggc ggcagcggcg cgtttgacac aggggggctt tgcctttctg gtcgatcggg
     3961 agcgttcact tgcttttccc ttgattgttc agggattgaa aagcccggat aaatcagttg
     4021 ttatcgccag caatagcctg ctcgaacatc tccatcgggt accgccgcct cctctgaaga
     4081 tcaccaccct cggcggctgg tgcctgaagg ctggcgggcg ttcaattgat ccccaattcc
     4141 ttcgccagcg ccgcgctgga gaactgctgg gcttcctgct catcagtccc gggcgccgcc
     4201 tgcccattga ccagattatt gactctttct ggccggaaaa agaatttaac gctgctcagg
     4261 ccctgcttca tcaggccacc tccaccctgc gccggacgtt ggaacccgat cttcccgaaa
     4321 aattcccttc ccgctatctg cgggtggatg atggcagtat tgccctggct ctgccaccgg
     4381 attcgcaggt ggactatgag acctttatga gccatatccg ctgcgccgat tgggatgctg
     4441 ccctggcctg ctgccccggc gaatttctgc cagaataccg ttatgccgac tgggctgaag
     4501 cgcaccgccg ctggctggca caggaatatc aacgcgcctt gttggcaaaa gcccgcgtct
     4561 ggctgtctga aaaacaattt ttgcccacgc tggatgcctg ccgtaagctt ctggctgctg
     4621 aaccctggca ggagcaggca gtcctgctgg gcatgcaggc ctgccttggc ctgggggata
     4681 ccgccggggc gctgcgcctt tacctaactc tggagaagac ccttcaaact gatctggggg
     4741 tagagccaca gcctgaattg cagacccttt atcgttccct gttaaagcgt tagccatttc
     4801 ccgctttttg tcagtcttct gtcagtggct gttggtataa agaatcaaca gtctgtccct
     4861 tgctgtcccc gcagatcgga tcaatcaggc ggctttgttc gcgcggtttg gttgaagaca
     4921 gcatcattcg attatccttt ggaggtctcc ccatgcttaa gtctcaaaat tcccgcccag
     4981 tcctctatct gacggctcta ctggcgctgg tcatatcgtt atgcctgaac ctggcgcccg
     5041 tccgggccag cagcttttcc caaaatgcct tgtctggtaa cccaaatgca ttcacctggc
     5101 aaaaggtcat tgcgccagat ggcacctcca aggatggctt cggtcattcc gccgccattt
     5161 cagacgacgg cagtacggcc attgtgggct cctactatat cgaatttggc atcacctacc
     5221 ccataggccc gggcggcgtg tatattttta ccaacgatgc cggtacctgg gttctgcagc
     5281 aaaaactgcc caccccggac atgcccgatt ctgacctgtt tggcttttcg gtcgcgcttt
     5341 ccgccgatgg gaacacagcc ttggtgggcg cccagggcgg caccggcccg gtggacaccc
     5401 aggttcctgg aactgcctat atcttcaccc gcagcgaggg cgtctggagc cagcaggcca
     5461 tgctcaccgc ctccgatcgc caggtaggcg accacttcgg cgtctcggtg gcgctctcag
     5521 acagcggcga taccgccctg attggcgcct acgtggatga tgtcggcgca aacaccaacc
     5581 agggctcggc ctatgtctat cagcgcagcg gaaacgcctg gaccgaacag gcgcacctga
     5641 ccagcaccga tgctgccgct ggcgattatt ttggacgctc cgttgccctc tccgcggatg
     5701 gcagcatggc tttgatcggt gccgacatga aaaccatagg cgcaaataca gcccagggtg
     5761 cggcttacat ttttacgcgc gccagtacta cctggagcca gcaaacccgg ctgaccgata
     5821 tcaatggcgg tgggtacgaa tggtttggcg taggggcggc cctgtcggct gacggtcaaa
     5881 ctgcgctggt tggcgcagat ggaaccggag cgtttgtgtt cacggcttcc ggcggaatct
     5941 ggacacagca ggctttgttg acggatggga gtgcatacct cgggtggggc aacagcattg
     6001 cgctttcggc tgatggtaat acagccctgg ttgaggatgg cttcacgttt acgcgtcata
     6061 gcggcagttg gatcgctggc gagcgtctgc tggcagatga tagcccttcc ggctttggtt
     6121 ctgcggtagc gctctccggc gatgcatccc ttgccctgat tggcgcaagc tacgccacca
     6181 ttggtgccaa tcagtcgcag ggcgccgcct acttcttcac caaaacatca tctggcccgc
     6241 aaacttcaat ttacctcccg ttaattgtgc gctgagataa ggcccttttt gtcagtcttc
     6301 cgtcagtcca cccgggtata aaaagacatg cgaaccgtcc tgaccgtcat cctgcgcctc
     6361 tttgtggata ccgatcaacc ggatgccgtg cgcggcgccc tgcaggggct tggcagc
//
LOCUS       DAOPVS010000107         6398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_12071_length_6398_cov_57.926060, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000107 DAOPVS010000000
VERSION     DAOPVS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12071_length_6398_cov_57.926060"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1935)
                     /locus_tag="PKL11_08395"
     CDS             complement(67..1935)
                     /locus_tag="PKL11_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNZ13662.1"
                     /translation="MSRKPGRQFSHRFRSSLRWPVLATLSALLLVFLSGASYSIYRLV
                     SQQEAQRWEERQLSETEYAVQIVSVFLDEQIRDLAIASRISADQLSSDTALRQEILNQ
                     QPAWLELVRVDATGKEIINLSRKAPLLNNPVTIPQSTWFVEASDGRFYVGNLNVSADN
                     EPYIIIAQPASDGGVIAALLNMNVLWEAVSNVSYGETGLAFVVNNRGHVIAHPNIDLV
                     LARTSIADTPQFEKILNSAVSREIFTTTNLAGEAVQAAAAQVPGSRWYIVTEVEQDEV
                     GRVSRNAAIAFVVFMLVVGFLGTLLINHLLIVLIFHPLQRLQAGIDRISQGDLDYTIQ
                     MRHQDEFGMVAQAFNSMAGSLKERQEQVLIARDQAQEANRFKSEMLAKVSHEFRTPLG
                     VIIGYGEMLTAGVYGEMKKDQTQKVNELVESAQGLNQLVNDLLDQARLEAGEIKLVRE
                     PVDLSELVNRIIQQMSYLAQIKKITLTSEIEREMPATLLGDEGRLQQILVNLVNNAIK
                     FTEKGGVHIRIMRPDADFWAIQVSDTGIGIPLSAQHTIFEPFRQVDGSVTRQRGGTGL
                     GLSIVRQLTLAMGGTIHLDSLPGRGSTFTILLPLILKEEKPHESPVISPPTPRTGH"
     gene            2166..2435
                     /locus_tag="PKL11_08400"
     CDS             2166..2435
                     /locus_tag="PKL11_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNZ13663.1"
                     /translation="MSNPDITARLKNAEGHLRGIQRMLDEDAYCIDVIRQIQAVQAAL
                     NKVTSIILNDHLNSCVITAIRGDDPAERERVLKEISEVFEASRKA"
     gene            2447..2653
                     /locus_tag="PKL11_08405"
     CDS             2447..2653
                     /locus_tag="PKL11_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNZ13664.1"
                     /translation="MNTLIYNIPAISCHHCVHTIKMEVGEIPGVQSVEGDPQTKQITI
                     VYQAPATPEQIEQLLTEINYPPTR"
     gene            2779..3225
                     /gene="rpiB"
                     /locus_tag="PKL11_08410"
     CDS             2779..3225
                     /gene="rpiB"
                     /locus_tag="PKL11_08410"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNZ13665.1"
                     /translation="MRIAVACDHGGFPLKETVVQTVLQAGHDVLDLGTNSPAAVDYPD
                     FAERAGRALQNGEADRAIVLCGSGVGACIAANKMKGVYAGVCHDVYSAHQGVEHDNMN
                     VLCLGARIIGIELAKELVAAFLNAKFSTEERHRRRVAKVLELEKKN"
     gene            complement(3237..4127)
                     /locus_tag="PKL11_08415"
     CDS             complement(3237..4127)
                     /locus_tag="PKL11_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ13666.1"
                     /translation="MAYYGGIEAGGTKFVCAIGKGPGEILAETRFPTTTPEETLGATV
                     RFFREAAQTHQIRLDAVGIGSFGPLDLDPASPTYGYITSTPKAGWADTPFVSLIEKAF
                     GVPVGWDTDVNAAALGEGLWGAAKGLDNFLYLTIGTGVGGGAVINGRPLHGLVHPEMG
                     HMLLPPRPDGFTGVCPFHKNCFEGLATGPSMAVHWGQPAQTLPADHPAWEEEAAVIAS
                     ALHGLICVLSPTRIILGGGVMQQPQLFPMIRQKVKESLNGYVQAEAILNGMDGYIVPP
                     GLAGKAGVLGAFALAQLALG"
     gene            4274..5452
                     /locus_tag="PKL11_08420"
     CDS             4274..5452
                     /locus_tag="PKL11_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ13667.1"
                     /translation="MDIFEKCFSYTDAKEAIARGVYPFFIPMSENEGTETIFHGRRLI
                     MCGSNNYLGLTTHPKVREAAKEAIDRYGTSCTGSRFLNGTLELHERLESELAEWVGKS
                     AALVFSTGMQVNLGTVSSLVGRDDIVILDKDDHASIVDGARLGYGKIERFRHNDMPHL
                     ERVLQSLPESAGKLIIVDGLFSMEGDLADLPNIAPLAKKYGARIMVDDAHGMGVLGGG
                     RGTAAHFGITGDVDLIMSTFSKSFASLGGFVAGDEDVIHYVKHHARALIFSASIPAPN
                     AAAALAALQVMREEPERIARVNQNAEKMRQGYRSLGFNIGTSCTPVVPIIIGDDDRTF
                     MMWRLLFDAGVFVNPVISPATPPGRQLLRTSYMATHTNEQLDRVLEIFAQVGKQVGII
                     "
     gene            complement(5516..>6398)
                     /locus_tag="PKL11_08425"
     CDS             complement(5516..>6398)
                     /locus_tag="PKL11_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ13668.1"
                     /translation="VLCYNTRLLCHYKGHPMIEITTDSCCDLSPELITKHQIRVINLN
                     VFINGHDYKDGELTQAELFALADESGELPKTSAPSISEFVNLFDTPNDKLFIGISAKL
                     SATLQNAIKGAEMANQPVRVIDSMNLSTGIGLLVLKAAELRDQGRTIDEIKEIIERTV
                     PYVRTAFVIDTLDYLYKGGRCTAMQHVVGSMLRLRPVIEVRADGTLGVKEKIGGSRKK
                     ALNSMLESFKRSLPGVDLHRVFVTHTSCEEDAEYLKAELLKLAPIEEVCITEAGATIA
                     SHCGPNTIGILYILKAE"
BASE COUNT         1473 a         1855 c         1578 g         1492 t
ORIGIN      
        1 atttcggtct gataatcggc taatgccagc gcttttgaaa atatttcggc gagctgtaca
       61 tcgtcctcaa tgaccagtgc gaggggttgg gggggagata acgggggatt catggggctt
      121 ttcctctttt aatatcaaag gaagcaaaat ggtgaatgtg ctgccgcggc ccggcagact
      181 gtccaggtgg atggtccccc ccatagctag agtcaattgg cgaacaatgg ataaccctaa
      241 cccggttccg ccccgctgtc gagttaccga accatcaacc tgccgaaaag gctcaaaaat
      301 agtgtgctgg gcgctcaacg ggatgccaat tcccgtgtcg ctgacttgaa tggcccagaa
      361 atccgcatct ggccgcatga tgcgaatatg gacgcctcct ttttctgtaa atttgatggc
      421 attattgacc agattgacca gaatttgctg caatcgtcct tcgtcaccca gcagggtggc
      481 gggcatttca cgttcaattt cgctggtcag cgtaatcttt ttgatctgcg ccagataact
      541 catctgttgg ataatccggt tgaccagctc tgacaggtcc accggctccc gaaccagttt
      601 gatttccccg gcttccagtc gcgcctggtc cagcaaatca ttcaccaact gattcaaccc
      661 ctgcgcgctc tcaaccagtt cattcacctt ttgggtctgg tctttcttca tctctccata
      721 gaccccggca gtcagcatct cgccatagcc aatgatgacc cccagcggag tccgaaattc
      781 atggctgacc ttggcgagca tctcagactt aaaccggttg gcttcctgag cctgatcgcg
      841 ggcaatcagc acttgttcct ggcgctcttt caggctgccc gccatgctgt taaaagcctg
      901 agccaccatg ccaaattcat cctggtggcg catctggatc gtgtaatcca aatccccctg
      961 gctgatgcgg tcaatgccgg cttgcagccg ctggagcggg tggaagatca gcacaatcag
     1021 caaatggttg atcaataatg tgcccagaaa ccccaccacc agcataaaga ccacaaaagc
     1081 aatcgctgca ttcctgctga cacgccccac ttcatcctgt tctacttctg tcacaatata
     1141 ccagcgtgat cctggcacct gcgcagccgc agcctgaacc gcctcgcccg ccagattggt
     1201 ggtggtaaag atttctctgc ttaccgcgct gttgaggatt ttttcaaatt gcggggtatc
     1261 cgctatgctg gtgcgcgcca gcacaagatc aatattggga tgagcaatga catggccgcg
     1321 gttgttcacc acaaaggcca gccccgtttc tccgtaactg acgtttgaaa cggcttccca
     1381 tagaacattc atatttaaca gcgcggcaat cacaccaccg tctgatgcgg gctgggcaat
     1441 gataatgtaa ggttcattgt ccgccgaaac attcaagttc ccgacataaa atcgcccatc
     1501 gctggcttcc acaaaccagg tggattgtgg aatggtcacc gggttattca acagcggcgc
     1561 tttgcgagac aggttgataa tctcttttcc tgtggcatct acccgcacca gttccagcca
     1621 ggcgggctgc tgattaagaa tttcctgtcg taacgccgta tcactggaca gttgatcggc
     1681 cgatatccgg ctggcaattg ccaggtctcg aatttgctca tcaagaaaca cagacacaat
     1741 ttgtaccgca tattcggttt cagatagctg ccgctcttcc cagcgctgcg cctcctgctg
     1801 gctgaccagc cggtaaatgc tgtagcttgc ccctgaaagg aatactaaca ggagcgcgct
     1861 taaggttgcc agtactggcc agcgcaaact ggatcgaaac cgatgagaaa actggcgacc
     1921 aggttttcgg gacatatcaa caacggccct ttacgatcgc ggaacgcaat aacagctaac
     1981 taagtgaata aaaggcgtgg aataacaacc agtagatatc atcataatta tatgccagct
     2041 taagaaattt gcgacctaca aatgcattac aattatttaa gaattcttca aaccggcttg
     2101 actctttctc tctaaaaggg tattatctcc ttaccccacc cacctggggg gggtaaggag
     2161 aacgcatgag taacccagac atcactgccc gcctcaaaaa tgccgaaggc catcttcgcg
     2221 gcatccagcg aatgctggac gaagatgcct attgtattga cgtcatccgt caaattcagg
     2281 cggttcaggc tgccttgaac aaagtcacca gcataatcct caatgaccac ctgaatagct
     2341 gcgttattac cgccatccgc ggggatgacc cggccgagcg cgagcgcgtt ctcaaagaaa
     2401 tcagcgaagt tttcgaggcc tctcgcaaag cataaggaga tttactgtga atacgcttat
     2461 ctataacatc ccggcaattt cttgccacca ctgtgtccac accatcaaga tggaagtcgg
     2521 cgaaatcccc ggcgttcaat ctgtagaagg tgacccccag accaaacaga tcaccatcgt
     2581 ttatcaggcc cctgccacgc cggagcaaat tgagcagctg ctcactgaga ttaattaccc
     2641 gccgactcgg taagtccatt gcggcgggcg tcttacagtc gcctgccgct tttttaatcc
     2701 ggatctcaca gtgaatttat gccacgtgtg gtagaatgac ctcaccccaa atttctgaaa
     2761 aagcgccagg agcctaatat gcgaattgcg gttgcttgtg atcacggcgg ttttccgctc
     2821 aaagaaacag ttgtccaaac agttcttcag gcgggtcatg atgtgcttga cctggggaca
     2881 aacagccccg ccgctgtcga ctatcccgac tttgccgagc gcgccggacg cgcccttcaa
     2941 aatggcgaag cagaccgcgc catcgttttg tgtggttcgg gtgttggtgc ctgcattgcg
     3001 gctaataaaa tgaaaggcgt ctatgcgggc gtctgccacg atgtctattc agcccaccag
     3061 ggtgttgagc acgacaacat gaacgtgctt tgcctgggcg ccagaatcat cggcattgaa
     3121 ctggcgaaag aactggttgc cgcttttctg aacgccaaat tctctaccga agagcgtcac
     3181 cgccgccgcg tggctaaagt tctggagctg gagaaaaaga actaacccgg ccagccttac
     3241 cccaaagcca gttgcgccag cgcaaaagcg cccagcacac cggccttccc ggctaaccct
     3301 ggcggcacaa tatagccatc catcccattc aaaattgcct cagcctgaac atagccgttc
     3361 aggctttctt ttaccttttg gcggatcatt gggaaaagct ggggttgctg catcacgccg
     3421 ccgcccagga taatgcgtgt aggcgaaaga acacagatca acccgtgcag cgctgaagca
     3481 atcaccgcgg cttcttcctc ccaggctggg tgatctgccg gcagggtttg cgcgggctgt
     3541 ccccagtgca ccgccatgga aggccccgtc gccaaccctt caaaacaatt tttgtggaag
     3601 gggcagaccc ccgtaaagcc atcggggcgg gggggcagca gcatatgccc catttctgga
     3661 tgcaccagtc cgtgcagcgg gcgcccgttg atcaccgccc cgccgccaac ccccgtgcct
     3721 atcgtcaggt agagaaaatt gtccagccct ttggcggctc cccacaaccc ttcgccgagc
     3781 gcggcggcat taacatcagt atcccagcca accggcacgc caaacgcttt ttcaatcagg
     3841 ctgacaaatg gcgtatcggc ccagccagct ttgggcgtgc tggtaatata tccgtaagtc
     3901 ggtgaggcgg ggtccaggtc cagcggtcca aagctgccaa tcccaacagc atccaatctg
     3961 atctggtggg tttgagccgc ttctctaaaa aatcgcaccg tcgcgcccag ggtctcttcg
     4021 ggcgtggtcg tcggaaaccg tgtctccgcc aggatttccc ccggcccctt gccaattgca
     4081 cacacaaact ttgtgccgcc agcctcaata ccgccgtaat atgccattgc ctgcctccaa
     4141 attgaactca attaatcata tcacaatcga aattcaaaca cagaaaaata aagcggtata
     4201 atcacttcaa ttcgcgctga cagggaacag cgcaatggta agtattccct gttatttttt
     4261 ggaggactca gtgatggata tctttgaaaa gtgtttttcg tacacagatg ccaaagaggc
     4321 aattgcccgg ggcgtctatc ccttcttcat tccaatgagt gagaatgaag gcaccgagac
     4381 gatttttcat ggccgtcgtc tgattatgtg cggatccaat aattatttgg gcctcaccac
     4441 ccaccccaag gttcgcgaag ccgctaaaga ggctattgac cgctacggca caagctgcac
     4501 cgggtcccgt ttccttaacg gcacgcttga actgcatgag cgccttgagt ctgagctggc
     4561 agaatgggtc ggaaaatctg ccgcccttgt cttctccacc ggcatgcagg tgaacctggg
     4621 tacggtcagt tcactcgtag ggcgggatga tatcgtcatc ctggataaag acgaccatgc
     4681 cagcattgtc gatggcgccc gcctgggcta tggcaaaatt gaacgcttcc gccataacga
     4741 tatgccgcat ctggagcgcg ttttgcagtc cctgccggaa tcggccggca aactgatcat
     4801 tgtggatggc cttttcagca tggaaggcga tctggctgac ctgcccaata ttgcccccct
     4861 ggccaagaaa tacggcgccc gcatcatggt ggatgacgct catggcatgg gcgtgctggg
     4921 cggtgggcgc ggcactgcgg cgcactttgg catcacaggt gatgtggacc tgattatgtc
     4981 caccttcagc aaatcctttg cctctctggg cggctttgtg gccggcgatg aagatgtcat
     5041 tcattatgtc aagcatcacg cccgggcgct catcttcagc gccagcatcc ccgccccgaa
     5101 tgctgccgcg gcgctcgcgg ccctgcaggt catgcgcgaa gaacccgagc gcatcgcccg
     5161 tgtcaaccag aatgccgaaa agatgcgcca gggctatcgc tccctcggct tcaatatcgg
     5221 cacctcctgc acgcccgtgg tacccatcat catcggcgat gatgaccgca cctttatgat
     5281 gtggcgtctg ctgtttgatg ctggcgtctt tgtcaacccg gtcatcagcc cggccacccc
     5341 tccgggccgc cagttgctgc gcaccagcta tatggccacc cacaccaatg agcagctcga
     5401 ccgtgtcctg gagatttttg ctcaggtcgg caagcaggtc ggcatcatct aaaacaaatc
     5461 accgggcggt tcatccgccc ggttttgatt tctcgatatc ttctggaatc aggttttatt
     5521 ccgccttcaa aatatacaaa atcccaatcg tattcggccc gcagtgtgag gcaatggttg
     5581 ccccagcctc tgtaatacag acctcttcaa tcggtgccag cttcagcagt tcagctttca
     5641 aatattcggc gtcttcctcg cagctggtat gagtcacaaa cacccggtgc agatccacgc
     5701 ccggcaggct tcgcttgaag ctttccagca tcgaattcaa cgccttcttg cgagaacccc
     5761 caatcttttc cttgacgccc agcgttccgt ctgcccgcac ctcaatcacg gggcgcagcc
     5821 gcagcatcga gccaaccaca tgctgcatgg cggtacagcg cccaccctta tacaggtagt
     5881 ccagggtgtc aatcacgaaa gcggtgcgca catacggcac tgttctttcg atgatttctt
     5941 taatctcatc aattgtccgt ccctggtcgc gcagttcagc cgccttcagc accagcagtc
     6001 caatccccgt tgacagattc atcgaatcaa taacccgcac cggctgattg gccatctctg
     6061 cgcctttgat ggcgttttgc agcgttgccg acagcttggc cgaaatgcca atgaacagct
     6121 tgtcatttgg cgtatcaaac aaattcacaa actcactgat ggatggcgcc gatgtcttgg
     6181 gcagttctcc agattcatct gccagcgcaa acagttctgc ctgtgttagc tcaccatctt
     6241 tataatcgtg cccattaata aagacgttga ggttgatgac ccggatttgg tgctttgtga
     6301 ttaactctgg gctgaggtcg cagcagctat cggtagtgat ttcaatcatg ggatgacctt
     6361 tataatggca taataatcgt gtattatagc ataatacc
//
LOCUS       DAOPVS010000108         6100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_12856_length_6100_cov_59.389082, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000108 DAOPVS010000000
VERSION     DAOPVS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12856_length_6100_cov_59.389082"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..434)
                     /locus_tag="PKL11_08430"
     CDS             complement(3..434)
                     /locus_tag="PKL11_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13669.1"
                     /translation="MEPSTLDPAVREAVTAEILSRLGRRGSEDDLVLWVCNQTGLQWD
                     AAQSLVDNVRLEHYPEIDRRRKPLLVALQVLIIVIGVILSVAPLLIWGEIVQLDGGSA
                     PQTVRHFKLAFDNAGGWVMIIFGVALVILGAWRRQQHQPSK"
     gene            complement(436..861)
                     /locus_tag="PKL11_08435"
     CDS             complement(436..861)
                     /locus_tag="PKL11_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13670.1"
                     /translation="MPTPQDLMPQIVARLGRDANEDSLVLWVCEETGLDWSAAQALIN
                     DAKVYHAGKIRRRRAPVRLALGGLILIAGIILTIYPLYTLVMQARQLQLTVGQALTLG
                     LGQFLDPGFTTALTLGVAMMVGSGLALGEELAHVSEKET"
     gene            complement(880..2805)
                     /gene="gyrB"
                     /locus_tag="PKL11_08440"
     CDS             complement(880..2805)
                     /gene="gyrB"
                     /locus_tag="PKL11_08440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNZ13671.1"
                     /translation="MTTNNANISSYGAKDIQVLEGLEAVRRRPGMYVGGTDQKALHHL
                     VYEVVDNSIDEALAGVCDRITVTIHPDSSITVEDNGRGIPVDVHPEKKKSALEVVMTT
                     LHAGGKFGHSSYKVSGGLHGVGVSAVNALSEWCEVEVRRDGKVYFQRYERGIPQEAVK
                     PIGKCDPRITGTRTTFHYDTQIFQGDVEFRFDTLATRFREMAFVTRQVQIRLVDERSS
                     HETSFYFDGGIRSFVRYLNRNREVLHPVFYVEKEIENIGIEVSVQYTDGYNESVYSFA
                     NTINTVDGGTHLTGLRMALTRVINDYARKTGLLKEADPNFTGEDTREGLTALVSIKHP
                     DPQFESQTKVKLMNPEVQTYVQQIVGEAFQSFLQENPQAARAIVQKNLTSARARDAAR
                     KARDLVIRKSALESLTLPGKLADCSERDSNKTEMYIVEGNSAGGSAKQGRDRHFQAIL
                     PLRGKILNTERARMDKLLGNEEIKSMISALGVGIGSSFDLSGLRYGRVIIMTDADVDG
                     SHIRTLLLTFFFRNMQPLIDEGHLYIAQPPLYRVAYRNQVRYAYTDADLERLLKEMGV
                     GSDKASIQRYKGLGEMNPQQLWETTMDPERRTLLQVSIEDAAEADRTFDMLMGEAVPP
                     RTRFIQTHARSVRNLDV"
     gene            complement(2827..3834)
                     /locus_tag="PKL11_08445"
     CDS             complement(2827..3834)
                     /locus_tag="PKL11_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13672.1"
                     /translation="MQAAPEKTPAPLPLPGIVPALVRGFNIAAANVHLVLLPVMLDAL
                     LWLGPRLGVEQVLTPALSYVSTNLLKAAQPAQVEAVNTALELLQTSISQFNLVSLLST
                     FPVGVPALTPMAETAATPYGAPLVTQVGGTLPLLGLTVLLIVIGWLLGSLYFGAIGRA
                     ATEAVEPFNLRTAARHFVQVAALTLMLLGLALLVTVPGALLVSLIAVVSPVVGQFLMF
                     FVIFLLVWLIVPLLFAPLGIFTHQMNAITALLNSWRLTRFYLPTGGMFIMTAAVLGLG
                     LDMLWTLPEASSWLLGVGILGHAFIYTALVAAAFVYFRGGMKVMQSRQTPRPPVERPT
                     L"
     assembly_gap    3864..3963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3990..4063
                     /locus_tag="PKL11_08450"
     tRNA            3990..4063
                     /locus_tag="PKL11_08450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4024..4026,aa:Arg,seq:gcg)
     gene            4162..5391
                     /locus_tag="PKL11_08455"
     CDS             4162..5391
                     /locus_tag="PKL11_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13673.1"
                     /translation="MNRTLKARLQKWLLTFNDPRWAFLQAALFYILISLLIETPFRLS
                     NAPHFQYLADAFLHGQLNLRVVPPTDHDLVFFQGNTYLYWPPFPAIVMMPFVALVGLH
                     FSDVFLVMMIGAFNVSLMALLLRAVNQRGLIPLSAVKRAWLVIFFMAGTVHTTLALTG
                     NVWSASQVISIAGLLVAYCAAVGLKGWKAYLFAGIGMAAAMSTRYTLILAGIWPAWYL
                     INETRKAEPARLLRYMLIGSLPILIAGGLLGWYNAARFGSPFDYGYAYHNMSVIFEEN
                     YARYGAFNLHYIPDNVYYQYIYYPFPVREDFRMGGSLFLLSPVFFAALYSLWQDRKTL
                     STWVLVITISLVNLPILLLMGTGWQQFGPRYTLDFTIPLLLLTAQGCRKLPTWALAAL
                     TLISAAHYIVGSIIFLP"
     gene            5388..>6100
                     /locus_tag="PKL11_08460"
     CDS             5388..>6100
                     /locus_tag="PKL11_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13674.1"
                     /translation="MMRPPTDLKAHKIVIVMPAYNAARTLQRTLDEIPPEFREHVILV
                     DDASRDHTAELARELGLTVFVHPQNRGYGGNQKTCYREALALGAEIVVMLHPDHQYDA
                     KVIPDLVYPIIRGEADAVFGSRMLGGHPREGGMPWWKYAANIFLTAAANLIFGRYLTE
                     IHSGFRAYSRKYLETVRYEDNSDDFVFDTEIIAQGMACNLFFRETPIVTRYFPEASSI
                     NFRRSTQYGFGVLWTLLRYG"
BASE COUNT         1126 a         1857 c         1819 g         1198 t
ORIGIN      
        1 ccctactttg agggctggtg ttgctgccgc cgccaggccc ccaggatgac cagtgcaacc
       61 ccaaagataa tcatcaccca gccgccggca ttgtcaaagg ccagtttgaa gtggcgaacc
      121 gtttgcgggg cgctgccgcc gtccagttgg acgatttctc cccagatcag caggggcgcc
      181 accgaaagga tgacgccaat gacaatgatc agcacctgca gggccaccag cagcggtttg
      241 cggcggcggt caatctccgg gtaatgctcc agccggacat tgtccaccag gctctgggca
      301 gcatcccact gcagcccggt ctgattgcaa acccacagca ccaggtcatc ttcgctgcca
      361 cgacgtccca ggcggctcaa aatttcagcg gtaacggctt cccgcacagc ggggtccagg
      421 gttgagggtt ccatcctatg tctccttctc actcacatgc gccagctctt cgcccagcgc
      481 caggccgctg ccgaccatca tcgccacccc cagggtcaag gcggttgtaa aacccgggtc
      541 caagaactgc cccagcccaa gggtcagggc ctgcccaaca gtcaattgaa gctgccgggc
      601 ctgcatcacc agggtataga ggggataaat cgtcaggata atgccggcaa tcaatatcag
      661 cccgcccagt gccagccgca ctggcgcccg ccggcggcgg attttgcccg cgtggtagac
      721 tttggcatca ttgatcaacg cctgggccgc agaccagtcc agcccggttt cttcacaaac
      781 ccacagcacc aggctgtctt catttgcatc ccgccccagg cgggccacaa tttgcggcat
      841 caggtcttgc ggggtgggca tgggcagatg acctgcgggt tacacgtcca ggttgcggac
      901 gctgcgggcg tgcgtctgga tgaagcgggt gcgcgggggc acggcctcgc ccatcagcat
      961 atcaaaggtg cggtcggcct cggcggcatc ttcaatgctc acctgcagca gggtgcggcg
     1021 ctcggggtcc atggtggtct cccagagctg ctgggggttc atttcgccca gacccttgta
     1081 gcgctggatg ctggctttat cggagcccac gcccatctct ttgagcaggc gctccaggtc
     1141 ggcatcggta taggcgtagc gcacctggtt gcggtaggcc acgcggtaaa gcggcggctg
     1201 ggcaatgtag aggtggcctt cgtcaatcag cggctgcatg ttgcggaaga agaaggtgag
     1261 cagcagggtg cgaatatggc tgccgtccac gtccgcatcg gtcataatga tcacgcggcc
     1321 gtagcgcagg cccgaaaggt caaaggaaga gccaatgccc acgcccaggg ccgagatcat
     1381 ggatttgatt tcttcgttgc ccagaagttt gtccatgcgg gcgcgctcgg tgttgaggat
     1441 cttgccgcgc agcggcaaaa tggcctggaa gtggcggtca cggccctgtt tggccgagcc
     1501 acccgccgag ttcccttcga cgatgtacat ctcggtcttg ttcgagtcgc gctccgagca
     1561 gtcggccagc ttgccgggca gcgtcaggct ttcgagcgcg ctcttgcgaa tgaccagatc
     1621 acgggccttg cgggcggcgt cacgggcgcg ggccgaggtc aggttcttct gcacaatggc
     1681 gcgggcggcc tgggggttct cctgcaaaaa cgactggaag gcctcgccga caatctgctg
     1741 cacataggtc tgcacctcgg ggttcatcag tttgaccttg gtctggcttt caaactgtgg
     1801 gtcggggtgc ttgatgctga ccagggcggt gagtccttcg cgggtatctt caccggtgaa
     1861 gttggggtcg gcttctttga gcaggccggt tttgcgggcg taatcgttga tcacgcgggt
     1921 gagggccatg cgcagaccgg tcaggtgtgt gccgccatcc accgtgttga tggtgttggc
     1981 aaacgaatag accgactcgt tgtagccatc ggtgtactgc accgaaacct caatgccaat
     2041 gttctcgatc tctttttcca catagaagac cgggtgcagc acttcgcggt tgcggttgag
     2101 gtagcgcaca aacgagcgga tgccgccatc aaaatagaac gaggtttcgt ggctgctgcg
     2161 ctcgtccacc aggcggatct gcacctgccg ggtgacaaag gccatttcac ggaagcgggt
     2221 ggccagggta tcaaagcgaa attccacgtc gccctggaag atctgggtgt cgtaatggaa
     2281 ggtggtgcgg gtgccggtga tgcgcgggtc gcatttgcca atcggtttga cggcttcctg
     2341 cgggatgccg cgctcgtagc gctggaagta aaccttgcca tcgcggcgca cttctacctc
     2401 gcaccactct gaaagcgcat tgaccgccga aacgcccacg ccgtgcagac cgcccgaaac
     2461 cttgtagctg ctgtgcccaa acttgccgcc agcgtgcagg gtggtcatca ccacctccag
     2521 cgccgatttt ttcttctcgg ggtgcacatc caccgggatg ccgcggccgt tgtcttccac
     2581 ggtgatgctg ctgtcggggt gaatggtcac agtaatgcgg tcgcagacgc ccgccagggc
     2641 ttcgtcaatc gagttatcca ccacctcgta caccaggtgg tgcagggctt tctggtcggt
     2701 gccgcccaca tacatgccgg ggcggcggcg gacggcttcg aggccttcga gaacttgaat
     2761 atctttggcg ccgtacgagg aaatattggc gttattggtt gtcattattc agggctccag
     2821 tcaaaattac agcgtggggc gttcaaccgg cggacgcggg gtctggcgag actgcatgac
     2881 cttcatgccg ccgcgaaaat aaacaaaggc tgcggccacc agggccgtgt agataaaggc
     2941 atggcctaaa atgcccacgc ccagcagcca ggaactggcc tcgggcagcg tccagagcat
     3001 atccaggccc aggccgagca ctgcggcggt cataatgaac atgccgccgg tcggcaggta
     3061 aaagcgcgtc aggcgccaac tgttcagcag cgcggtgatg gcgttcatct ggtgggtgaa
     3121 gatgcccagc ggcgcaaaca gcagcggcac aatcagccag accagcagga agatcacaaa
     3181 gaacatcaga aactgcccaa caaccggact gaccaccgca atcagagaga ccagcagcgc
     3241 ccccggcacg gtgaccagca gcgccagccc cagcagcatc agcgtgaggg cggccacctg
     3301 cacaaagtgg cgggcggcgg tccgcagatt gaagggttcc accgcttcgg tggcggcccg
     3361 gccaatggcg ccaaagtaga ggctgcccag cagccagccg atgacgatga gaagcaccgt
     3421 gagacccagc agcggcaggg tgccgcccac ctgcgtgacc agcggcgcgc cgtagggtgt
     3481 tgccgccgtc tcagccatgg gcgtcagtgc gggcacgccg accgggaagg tggacagcag
     3541 gctcaccaga ttgaactgcg atatcgaggt ctgaagaagc tcgagtgcgg tattgacggc
     3601 ctctacctgg gcgggctgag cggccttaag caggttggtc gaaacatagc tcagggcagg
     3661 ggtcagcacc tgctccaccc ccaggcgggg gcccagccag agcagcgcgt ccaacatcac
     3721 gggcagcagg acgagatgca cattggccgc ggcgatatta aagccgcgca ccagggcagg
     3781 cacaatgccc ggcagcggca aaggggcggg tgttttttca ggagcagctt gcataacaca
     3841 tgaattttac cacgttccgg gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnncatttga ggtatattaa agcccgcctg cccctgtagc gcaattggat agagcgtttg
     4021 cttgcggagc aaaaggtttg gtgttcaaat caccacaggg gcacagcaaa accgggtggc
     4081 gcttatgcgc tgcccggttc tgatttaagc ccggcttcgg tatggcgccc cggcgggcac
     4141 ctgttgtata atcacccctg tatgaaccgc accctcaagg cccggctgca aaagtggctg
     4201 ctcaccttca acgacccccg ctgggcgttt ttgcaggcag ccctctttta tatcctcatc
     4261 agcctcctca tcgagacgcc tttccggctc tccaacgcgc cgcatttcca atacctggcc
     4321 gatgccttcc tgcacgggca gttgaatctg cgcgttgtgc cgcccaccga tcacgacctg
     4381 gttttcttcc agggcaacac ctatctctac tggccgccct tcccggcaat tgtgatgatg
     4441 ccgtttgtgg cgctggtggg gctgcatttc agcgatgtct ttctggtgat gatgatcggg
     4501 gcgttcaacg tcagcctgat ggccctgctg ctgcgcgctg tcaatcagcg cggcctcatc
     4561 ccgttatcgg cggtcaaacg tgcctggctg gtgatcttct tcatggcggg cacggtgcac
     4621 accaccctgg ccctgacggg caacgtctgg tcggcctcac aggtgatcag catcgccggg
     4681 ctgctggtgg cttactgcgc cgcggtgggc ctcaaaggct ggaaggccta cctgttcgcc
     4741 ggcatcggca tggccgcggc catgtccacc cgctacacgc tgattctggc cggcatctgg
     4801 ccggcatggt atctaattaa tgaaacccgc aaggccgaac ccgcccggct gctgcgttac
     4861 atgctcattg gcagcctgcc catcctcatc gccggcgggc tgctgggctg gtacaacgcc
     4921 gcccgcttcg gcagcccgtt tgattatggc tacgcttacc ataacatgtc ggtgattttt
     4981 gaagaaaact acgcccgcta tggcgcgttc aacctgcact acatcccaga taacgtctat
     5041 tatcagtaca tttactaccc cttcccggtg cgcgaggatt ttcgcatggg cggcagcctg
     5101 ttcctgctca gcccggtctt ttttgccgcg ctctacagcc tgtggcagga tcgcaaaacc
     5161 ctctctacct gggtgctggt gatcaccatt tcgctggtca atctccccat tttgctgctg
     5221 atgggcacgg gctggcagca gtttggcccg cgctacacgc tcgatttcac catccccctg
     5281 ctgctgctga ctgcgcaggg ctgccgcaaa ctgcccacct gggccctggc cgcgctgacg
     5341 ctgatctcag cggcccatta tattgtcggg tcaattatct tcctgccatg atgcgccctc
     5401 caaccgatct caaagcccat aagattgtga ttgtgatgcc cgcctacaac gccgcccgga
     5461 cgctgcagcg cacgctggat gaaattccgc cggagtttcg cgagcatgtg attctggtgg
     5521 acgatgcctc gcgcgatcat accgccgagc tggcgcgcga actgggcctg actgtctttg
     5581 tgcacccgca gaaccggggc tacggcggca atcagaaaac ctgttaccgc gaggcgctgg
     5641 cgctgggcgc cgagattgtc gtcatgctgc acccggatca tcaatatgat gccaaagtga
     5701 tccccgacct ggtgtacccg atcatccgcg gcgaggccga cgccgttttt ggctcgcgca
     5761 tgttgggcgg gcacccgcgt gagggcggca tgccctggtg gaaatacgcc gccaacatct
     5821 tcctgaccgc cgcggccaac ctgatttttg gccgctatct caccgagatc cactccggct
     5881 ttcgggccta ctcgcgcaag tacctcgaga cggtgcgcta cgaagacaac agcgatgact
     5941 tcgtcttcga taccgagatc atcgcccagg gcatggcctg caacctgttc ttccgtgaga
     6001 cacccatcgt cacgcgctat tttcccgagg cctcgtccat caacttccgg cgcagcacgc
     6061 agtatggctt tggcgtgctg tggacgctgc tgcgctacgg
//
LOCUS       DAOPVS010000109         6059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_12954_length_6059_cov_33.688874, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000109 DAOPVS010000000
VERSION     DAOPVS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12954_length_6059_cov_33.688874"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1975)
                     /locus_tag="PKL11_08465"
     CDS             complement(<1..1975)
                     /locus_tag="PKL11_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNZ13675.1"
                     /translation="MSVIGQSVARIDAVGKVRGETLYPGDFNLPEQTYMKVLFAGHPH
                     AIVRSIDTTEAESTPGVIAVFTAKDVPVNEYGLGVPDQPVLCGPGSTNPYGDRVRFVG
                     DHVALVVAETEEIAAQACAKIRVDYEDLPVMTDSRKAMEPDAFLLHPERGSNVFCRFR
                     IRKGDVDAAFAEADVIVTGEYETPAQEHAYLQPEAGISYIDEDGRITVVVAGQWTHED
                     QEQIAHSLGLPLDQIRVIYPAIGGAFGGREDMSVQIILALAVWRMNQRGINRPVKIIW
                     SREESIIGHHKRHPYSIRTRWGATRQGKLVAAEVEIVADGGAYVYTSPKVLGNATIMC
                     TGPYEIPNVKVDSCAVYTNHLPNGAFRGFGGPQAAFSAELQMNKLAEILGMDPIEIRM
                     KNVLREGSLLSVGTPLPQGVTIAQVVERCAEEAGWKNTPDGWRLSHGLESSAPHKRRG
                     LGFACAFKNVGFSFGAPEQCTATVELYGSTEIERAVLYHSGADVGQGAHTVFAQMAAE
                     ALGIPMSRVHLVVSDTAMTPPSGSASASRMTFMAGNSIKGACERALERWRAEERPVKL
                     TYQYRPPATTPLDPETGKSNPNFAYGYVAEAIDLEVDLETGEIRVLNVVCADDVGHAL
                     NPMLVQGQVEGAVVQGLGYAVMEDFRQPGGIPQT"
     gene            complement(1991..2380)
                     /locus_tag="PKL11_08470"
     CDS             complement(1991..2380)
                     /locus_tag="PKL11_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13676.1"
                     /translation="MGKHYGGGLRQADPGPSRPDIHPVWRGIGWVLMILTPILGYFGA
                     LALIEANQVNEWFRIPKGLIYHNEISFLPHDPLLIVKALLTIIVAFILFFLFQLITMI
                     LYRITAPPQYGPFDVPRQAFRGKKKSR"
     gene            complement(2373..3812)
                     /locus_tag="PKL11_08475"
     CDS             complement(2373..3812)
                     /locus_tag="PKL11_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNZ13677.1"
                     /translation="MWDQYISAANIEEVISALDQYREKARVVAGGTDLILEMERGVRP
                     DVKILVDITRIPGLDQIVLDDQQWIHLGPRVTHNDCASSKLIREMAYPLARAAWEVGS
                     PQIRNRGTIAGNVITGSPANDTISPLMALGAQVVLRSTSGTRIVPLEKFYLGVRRTTM
                     QSDEALVDIRFPALQQNQRGTFIKYALRHAQAISLVNAAVIVTIENQKITQASITLGS
                     VAPTIIHAVEAETYLVGRSLTESVIAKAAKLASDAARPITDVRSSASYRRAIVEVIVT
                     RALNAILNKNETLGFPVEPVLLSGGLDRRKNGTVKQSQMHHADTPIVTTINGKVYTFE
                     TGQNKTLLRLLREDAGLIGTKEGCAEGECGACTVYLDGMAVMSCMVPAPRAHGAEITT
                     VEGISEGDVLHPVQQAFVEKGAVQCGYCTPGFVMSAAKLLEEYPQPSREQIQQAITGN
                     LCRCTGYYKIVEAIELAALLKQQGGDNHG"
     gene            complement(3825..4616)
                     /locus_tag="PKL11_08480"
     CDS             complement(3825..4616)
                     /locus_tag="PKL11_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNZ13678.1"
                     /translation="MTSILEAVADLEKNNTAGALCTIVRTRGATPRREGCKMLVYADG
                     QILGTVGGGEVENRVKAEALDSLRDGRTRLLEYSMIDPSKGDVGVCGGTVEVYVEPIL
                     PRPSVIVIGGGHVGKAVVQLAHWLGFLVGVSDDRIELCTPDTHPDADFFYPCTLSQLP
                     TMMNITPQVSLVLTTRGSDVDIEGLPALLQTDAGYIGVIGSRRRWSFTKQALLNAGMS
                     EDALCRVHSPMGLELNAETPEEIALSILSEIVMLRYRGTGKSMQA"
     gene            complement(4620..>6059)
                     /locus_tag="PKL11_08485"
     CDS             complement(4620..>6059)
                     /locus_tag="PKL11_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNZ13679.1"
                     /translation="GTITANEMTVLTDTGAYGCHALTVTGNTGHKSMALYVGDGKYRQ
                     SPNIRFYADVVYTNTPPAGAYRGYGVPQGYWAVERHMEKIARSLALDPIEFRLKNALR
                     PGELHPFSTAWSEGREPRPEIIHTVGLEECVRQGRAVIGWDDKFSVDAWHQVPNKPHL
                     RHGIGIAMMMQGTAIPYLDMGAASLKMNDDGSFNLLIGATDLGTGSDTVLAQMVAEVL
                     GVELEDILVYSSDTDFTPFDVGAYASSTTYISGGAATRAAQQVAEKICQRAAKMLSEN
                     GPAIEAEQIKLSNRRAAAPDGRFVTLFDLAHNALHHNDQEQIMAVASYMSPSSPPPFA
                     AQFAEVTVDVETGQVTVDRLLMAVDGGVIVNPVTAAGQIEGGMAQALGYAVCEEMRYD
                     ADGRARETDLRDYHIFRADEMPRMEVLFIETYEPSHPFGVKAVAEIPLDGVAPAVGNA
                     ILDACGANVDQNPATPERIWRALQSLKEG"
BASE COUNT         1472 a         1670 c         1499 g         1418 t
ORIGIN      
        1 tggtttgcgg aataccaccc ggctgccgga agtcttccat gacggcatac ccaagccctt
       61 gcacaaccgc accctcaacc tgcccctgca ccagcattgg atttaatgca tgaccgacat
      121 catcagcgca aacaacgttt agaacgcgaa tttcgccagt ttcaaggtca acttcaagat
      181 caatcgcttc ggcaacatag ccgtatgcaa aattggggtt cgacttccct gtctccggat
      241 caagcggtgt tgtggcgggc ggacggtact gataagtaag cttaaccggg cgttcttcgg
      301 ctctccagcg ctctaaagcg cgctcacagg cacccttaat agaattaccg gccataaaag
      361 tcattctgga agctgaggcg ctgccagacg gcggcgtcat ggctgtatcc gaaaccacaa
      421 gatgcacccg ggacattgga attcccagag cttcggctgc catttgagcg aatacggtat
      481 gcgcaccctg gcctacatct gcaccagaat gatacaaaac agcgcgctcg atttctgttg
      541 atccgtacaa ttccactgtc gcggtgcatt gctcgggagc gccgaacgag aagcccacat
      601 ttttgaaggc gcaggcaaac cccaatcccc ggcgtttgtg cggtgcggag ctttccaatc
      661 catggctcaa acgccaacca tcgggcgtgt ttttccaacc cgcctcttca gcgcaacgtt
      721 ctaccacctg ggcaatggtc acgccttgcg gaagcggcgt gccaaccgaa agaagagagc
      781 cttcgcgcaa gacgtttttc attcggattt ctatggggtc catccccaga atttctgcca
      841 atttattcat ttgcaattca gcagaaaatg cggcttgtgg gccgccaaat ccacgaaacg
      901 cgccattggg caagtggttg gtgtagacag cgcaagaatc caccttaaca ttcgggattt
      961 cgtatggacc ggtgcacata atggtagcat ttcccaacac cttaggcgag gtgtaaacat
     1021 acgcaccgcc atcggcaaca atttccactt cggcagcgac cagcttgccc tgtcgggttg
     1081 cgccccaacg agtgcggatt gaatacgggt gacgcttatg atgcccaata atcgattctt
     1141 cgcggctcca gatgattttg acgggacggt taataccgcg ttgattcatt cgccagacag
     1201 ccaacgccaa aataatctga accgacatat cttcacgccc gccgaaggca ccgccaattg
     1261 ccgggtaaat cacacgaatt tgatccagag gcagccccag tgagtgagca atttgctcct
     1321 ggtcttcgtg tgtccattga ccggcaacga caaccgttat tcgcccatcc tcgtcaatat
     1381 agcttatgcc tgcttcgggt tgaagataag catgctcctg ggcgggggtt tcgtattctc
     1441 ctgtaacaat gacatcagcc tcagcaaagg cagcatccac gtcgcctttg cgaattctaa
     1501 agcggcaaaa aacatttgaa ccgcgctcgg ggtgcagtaa aaacgcatca ggctccatcg
     1561 ccttgcgcga gtcggtcatc acaggcagat cctcataatc aacccggatt ttggcgcaag
     1621 cctgggctgc aatttcctcg gtctcagcga caaccagagc cacatgatcg cctacaaatc
     1681 ggacccggtc gccgtacgga ttggttgagc ctgggccaca aagtactggt tgatcaggta
     1741 cacccagtcc atactcatta acgggcacat cttttgcggt gaacactgca atcacaccgg
     1801 gggtagattc agcctcggta gtatcaattg aacgaacaat ggcatgggga tgtccggcga
     1861 acaaaacctt catgtaggtc tgctcaggca gattgaaatc gccggggtac agggtttctc
     1921 cgcgcacctt tccaacggca tcaatgcgag cgacagattg tcctattaca ctcatgttca
     1981 actcctcgga ttaacggctc tttttctttc ctcggaaagc ctgccgaggc acatcaaagg
     2041 gaccgtactg cggcggggct gtaattctat aaaggatcat cgttatcaac tggaataaga
     2101 agaagaggat aaacgcaacg atgattgtca gcaaggcttt aacgatcagg agcgggtcgt
     2161 ggggcaaaaa gctgatttcg ttatgataaa tcaggccttt tggaatgcgg aaccactcat
     2221 taacttgatt ggcttcaatc aaggctaacg caccaaaata ccccaaaatc ggggtcagta
     2281 tcatcaaaac ccagccaatc ccacgccaga cagggtgaat gtcagggcgg gatggacccg
     2341 ggtcggcctg tcgaagacca ccaccataat gtttacccat gattatctcc tccttgttgt
     2401 tttaataaag ccgccaactc aattgcctca acaattttgt aatagcctgt acaccggcaa
     2461 agattgccag tgattgcctg ttgaatttgc tcgcgactgg gttgaggata ttcttccagt
     2521 aatttcgcgg ccgacataac aaatccgggt gtacagtaac cgcattgcac ggcacctttt
     2581 tctacaaatg cctgctgcac cgggtgcagc acatcacctt cagatatccc ctccacagtc
     2641 gtgatttctg cgccgtgagc tcggggcgcc gggaccatgc agctcatcac agccattccg
     2701 tccagataaa cggtacaggc gccacattcg ccctcagcac agccttcttt agtgccaatc
     2761 aggccagcgt cctctcgcaa taagcgcaaa agggttttgt tctgcccagt ttcaaacgta
     2821 tacaccttac cattgattgt ggtgacaatc ggggtatcgg catgatgcat ttgggattgt
     2881 ttgaccgtgc cgtttttccg gcgatctaat ccgccactta atagcactgg ttcgacaggg
     2941 aagcccaagg tctcgttttt atttagaatg gcgttgaggg cgcgggtgac aatgacctca
     3001 acaatagcac ggcggtatga ggccgaactg cgtacatccg taattgggcg ggctgcatcc
     3061 gatgccagtt tcgcggcttt tgcaatcaca ctttcggtca gagaacgccc caccagataa
     3121 gtttcagctt ccacggcatg gatgattgtc ggcgcaaccg atcccagtgt aattgaagcc
     3181 tgggtgatct tctgattttc aatcgttaca atcacagcgg cattaaccag ggagatggcc
     3241 tgtgcatgac gcaaagcata cttgatgaat gttccgcgct gattttgctg caaggccggg
     3301 aagcgaatat caaccagggc ctcatcagac tgcatcgtcg ttcggcggac acccagatag
     3361 aatttttcca gcggaacaat gcgggtgcca ctggtggaac gcaagacaac ttgtgcacca
     3421 agcgccatta atggcgagat ggtatcattt gcaggtgagc ctgttattac attgccggca
     3481 atcgtccctc ggtttcggat ttggggagaa ccaacttccc atgcggcccg tgccagcgga
     3541 tatgccatct cgcggattaa tttggagctg gcacaatcgt tgtgtgttac ccgcgggcca
     3601 agatggatcc attgttgatc gtccagcaca atttgatcca gtccaggtat gcgcgtgata
     3661 tcaaccagga tcttgacatc gggtctgaca ccgcgctcca tttccaaaat aagatcggtg
     3721 cccccagcaa caacgcgagc tttctcccgg tattgatcca gggcagagat gacttcctct
     3781 atgtttgctg cactaatata ttgatcccac atatcgactc ctgcttaagc ctgcatagat
     3841 ttaccggtgc cgcgatatcg caacatgaca atttcagaca atatgctcag ggcaatctct
     3901 tcgggtgtct cagcatttag ttccagtccc atgggagaat ggacgcggca aagagcatcc
     3961 tcagacatcc cggcattcag caatgcctgt ttcgtaaatg accatcgccg gcgggaacct
     4021 ataacgccga tatatcctgc atctgtttgt aataacgctg gcaacccttc gatatccaca
     4081 tcggagccgc gagttgtcag tacaagagat acttgcggcg taatattcat catggtcggg
     4141 agttgcgaca gcgtgcacgg ataaaagaaa tctgcatctg ggtgtgtatc tggcgtacat
     4201 aattcaatgc gatcgtccga aacgccgacc agaaaaccca gccagtgcgc caattgaacc
     4261 acagcttttc caacgtggcc accgccaatc acaataactg aagggcgcgg gaggataggc
     4321 tcgacataaa cctcgactgt gccaccgcaa acgccgacat ctcctttgga tggatcaatc
     4381 atactgtatt ccagtaaacg cgttcggcca tcccgaaggg aatcaagcgc ttctgcttta
     4441 acccgattct ccacctcgcc gccgccaacg gtacccaata tttgtccgtc ggcatagacg
     4501 agcattttgc agccttcgcg ccggggagtt gctccgcggg tgcgcacaat ggtacacaaa
     4561 gctccggcgg tattattctt ctccaggtca gcaacagcct caagaattga ggtcatattt
     4621 cagccttctt tcagggattg cagtgcgcgc cagatccgtt ctggtgtggc aggattctgg
     4681 tctacatttg caccacacgc gtccaaaatg gcatttccaa ccgcaggagc cacgccatcc
     4741 agcggaattt cggcaacggc ctttacgcca aacggatggg atggctcata tgtctcgata
     4801 aacaatactt ccattctcgg catctcatcc gctcggaaga tatgataatc tctcagatca
     4861 gtctctctgg cgcggccatc cgcatcataa cgcatttctt cgcaaactgc atatcccaga
     4921 gcctgtgcca taccgccttc aatttggccg gcagcagtca ccgggttgac aattacacca
     4981 ccatctactg ccatcagcaa tcgatcaacg gttacctgtc cagtttcaac atccaccgtc
     5041 acttcggcaa attgcgctgc aaaggggggc ggggaagacg gagacatgta agaagcaact
     5101 gccatgattt gctcttggtc attatggtgc aaggcgttat gtgccaggtc aaacagggtg
     5161 acaaagcgtc catcaggcgc tgcggcacgg cgattggaca gctttatttg ttcggcttct
     5221 attgccgggc cattttcgct taacatttta gcggcccgct ggcagatttt ttcggcaacc
     5281 tgctgggcgg ctcgtgtggc tgcgccacct gatatataag tggtgctgga ggcataggcg
     5341 cccacatcaa agggggtgaa gtctgtatcc gatgaataga ccaggatatc ttcaagttca
     5401 acgcccagaa cctcggcaac catctgtgcc agaaccgtat cagatcctgt tccaaggtca
     5461 gtcgcgccaa tcaacaggtt gaaagagcca tcgtcattca tcttgaggct ggcagcaccc
     5521 atatccaggt atggaatggc ggtcccttgc atcatcatgg caatgccaat accatggcgc
     5581 aggtggggtt tgttggggac ctgatgccag gcatcaacgc taaatttatc gtcccaacca
     5641 attaccgccc gaccctgccg gacgcattct tccaggccaa cggtatggat aatttcgggg
     5701 cgtggttcgc ggccttcact ccaggcggtg ctaaaaggat gcaactcgcc ggggcgcaaa
     5761 gcattcttca accgaaattc aatcgggtca agcgccagcg aacgagcaat tttttccatg
     5821 tgccgctcga ctgcccagta tccctgtgga acaccgtagc cgcggtaagc gcccgccggc
     5881 ggtgtattag tatagacaac atctgcgtaa aaccggatgt tgggcgactg gcgatattta
     5941 ccgtcgccaa catataaagc cattgattta tgaccagtgt taccagtgac cgttaaggca
     6001 tgacatccat aagcgccggt atctgtcaga acggtcatct catttgctgt aatcgtgcc
//
LOCUS       DAOPVS010000110         5977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_13226_length_5977_cov_59.827592, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000110 DAOPVS010000000
VERSION     DAOPVS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13226_length_5977_cov_59.827592"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..444)
                     /locus_tag="PKL11_08490"
     CDS             complement(4..444)
                     /locus_tag="PKL11_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13680.1"
                     /translation="MKSPKIFLLASILLLTLSACAPAATPDPAAGDRQSLPTVAPLSA
                     GIQIIGGVVLSGGDQSPPGLMGGPHTFVYQVRLDSGEEINVTYTTLPPSPAAADQPSP
                     RLTFYAGVINVGDYLTARGDYNVETQTLTVALADDYIETFAQKP"
     gene            579..2447
                     /locus_tag="PKL11_08495"
     CDS             579..2447
                     /locus_tag="PKL11_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNZ13681.1"
                     /translation="MNKPFTYPLSRPGALEQAAIALLGGFLLAAAGALIAFVGFQALY
                     IGRIFPGVSVAGVNVGGLTPQAAADKLLIEVTYPQTGQILFQDAAAQHSWLATPMQLG
                     LFLDADASAQAAFRVGRTGDLWQRLDEQWQARQGGRAASPALIFDQRLATQFLNSIAQ
                     EVDRPVIEASLGVQGTEVMVNAGQPGRAVDISATLEALSLQLQSLQSGVVPVVVAETQ
                     PVILDASAQAEIARTMLSQNLTIQLPGGETDAGPWVFEPATLAEMLTIERVETETGAQ
                     YQVALDSEALITFLANIQTAVARSPENARFIFNDDTRLLEVIQPAVIGRSLMVDESLA
                     AIQQQTTAGQHTVELMIDYINPTITNETTGAELGITELVSSQTSYFRGSSRDRVNNIQ
                     IASAQFHGLLIPPGATFSMAEALGDVSLDNGYAEAMIIVGGRTVKGVGGGVCQVSTTL
                     FRTAFMGGYPIAERHAHAYRVYYYEQNSGGYNDALAGLDATVFVPLVDFRFTNDTPYW
                     LLMETYVNPSNYSLTWKFYSTSDGRSVEWHTTGPTNIVEPPEPKYIENPELSEGQIKQ
                     VDWEAEGADISVTRIVWRNGEIYFQDSVYTHYQPWQAVYEYGPGTEIPTPEPTPEE"
     gene            complement(2453..3103)
                     /locus_tag="PKL11_08500"
     CDS             complement(2453..3103)
                     /locus_tag="PKL11_08500"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNZ13682.1"
                     /translation="MDEFSAARERMVQQQIIRRGIESPRLLTALRQIPRHRFIPPESQ
                     EYAYADGPLPIGLGQTISQPYIVALMTDLLQLDGSETVLEVGTGSGYQAAILSRLAAR
                     VYTVERHVSLAEQARQILAELGCVNVQVQIGDGSLGWPAAAPYAGILVTAAAPAVPPA
                     LLNQLAPGGRLVLPVGPRFNQVLQLWQNTTAGLVSEDILPVAFVPLCGQAGWPTPD"
     gene            complement(3187..4260)
                     /gene="mutY"
                     /locus_tag="PKL11_08505"
     CDS             complement(3187..4260)
                     /gene="mutY"
                     /locus_tag="PKL11_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNZ13683.1"
                     /translation="MSAELAEKLLAWYAVNARTLPWRGHPDPYAVWVSEIMLQQTQVD
                     TVSPYFERWMARFPTLEALAAASEQEVLALWEGLGYYSRARNLWRAARQVWEAGGQMP
                     RTAADLALLPGVGRYTAAAIASMAFGQDEAALDGNIRRVLARVFNVTLPARSPEGERQ
                     LWALAQQHLPPGRAGDYNQALMDLGSQICTPRAPACLVCPLQALCQAAALGLQAERPV
                     MSARPPVPLWTVTAAVIWRADRVLIARRPSRGLLGGLWEFPGGKLEPGETLPEGLRRE
                     IAEELAVEIAVGAELGVYRHAYTHFKVVLHAFACTLVTGEPQPQVASELCWALPAELP
                     DFPMGKIDRQIAHRLLADSQKSA"
     gene            complement(4267..4569)
                     /locus_tag="PKL11_08510"
     CDS             complement(4267..4569)
                     /locus_tag="PKL11_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13684.1"
                     /translation="MPVKLIMSWDISPDHEQEYFEFVTSNFIPGVQRLGFELSDAWAT
                     VYGSQPQIMVGVVLPTLSKARQVMRSTEWVNLHNQLQDYVLNYSAKLVNARNGFQF"
     gene            complement(4583..5245)
                     /locus_tag="PKL11_08515"
     CDS             complement(4583..5245)
                     /locus_tag="PKL11_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ13685.1"
                     /translation="MEITWYGHSCFRLTERSLATVVTDPYDSKQVGYAPLKLKADVVT
                     VSHASPGHNCLSAVKGDPYQITGPGEFEIGGVFITGIQTNGHTKKTEDEPRNTLYLLD
                     YNGITVVHLGDLNRVPSQTEVEALGPVHVALVPVGGGGSLTAAKAAEMISLLEPNIVI
                     PMHYHIPETTAQMEPLSKFLKEMGLSSVQPQPSYKVTSLSALPDETQVIVLEYQNQNQ
                     NV"
     gene            5485..5557
                     /locus_tag="PKL11_08520"
     tRNA            5485..5557
                     /locus_tag="PKL11_08520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5518..5520,aa:Thr,seq:tgt)
     gene            5629..5712
                     /locus_tag="PKL11_08525"
     tRNA            5629..5712
                     /locus_tag="PKL11_08525"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5663..5665,aa:Tyr,seq:gta)
     gene            5769..5841
                     /locus_tag="PKL11_08530"
     tRNA            5769..5841
                     /locus_tag="PKL11_08530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5802..5804,aa:Thr,seq:ggt)
BASE COUNT         1262 a         1680 c         1889 g         1146 t
ORIGIN      
        1 tccttaaggt ttttgagcaa aagtctcaat gtaatcatcg gcaagcgcaa ccgtcagggt
       61 ctgggtttcg acattatagt cgccccgggc ggtcaggtaa tcgccgacgt taatgacccc
      121 ggcatagaag gtcagtcggg gggaaggttg atccgcggca gccggggaag gcggcagggt
      181 ggtgtaggtg acgttgattt cttcgccgct gtccaggcgg acctgataaa caaaagtgtg
      241 cgggccgccc atcaagcccg gaggggactg gtcgccgccg ctcaacacta cgccgccaat
      301 gatttgaata cccgccgaca ggggcgctac cgttggcaga ctttgccgat caccggcagc
      361 gggatctggc gtggcggcag gtgcgcaggc cgacagcgtc agaagcaaaa ttgacgccag
      421 taaaaagatt tttggggatt tcataaaact cctttggaaa aacaagacag gctatttgat
      481 tataacgagc gccgattcgt ttgacaatcc ggaacaaatc tgtgacaaat ttcgtcttat
      541 tttcaaattt gaaatcgagg ctataattga gtgtaaccat gaataagccc ttcacttatc
      601 ctctttcacg acccggtgcg ctggaacagg ccgccattgc cctgctgggt gggtttttgc
      661 tggctgctgc cggtgcgttg attgcttttg ttgggtttca ggcgctgtat attgggcgga
      721 ttttccccgg tgtgagcgtt gccggtgtga acgtgggcgg gctgacgccg caagccgcgg
      781 cagataaact gctgatcgag gtgacctacc cgcaaaccgg gcaaatcctt ttccaggatg
      841 cggcggcgca gcattcgtgg ctggcaaccc ccatgcagtt ggggctgttt ctggatgccg
      901 atgccagtgc gcaggcggct ttccgggtcg ggcgcacagg agatttatgg cagcgattgg
      961 atgagcagtg gcaggcccgg caggggggca gggcggcatc accggcgctg atttttgacc
     1021 agcgcctggc cacgcagttt ttgaacagca ttgctcagga agtggaccgt ccggtcattg
     1081 aggccagcct gggggtgcag ggcaccgagg taatggtcaa tgcggggcag cccggacggg
     1141 cagtggatat ttctgccacg ctggaagccc tgagcctgca attacaaagc ctgcaaagcg
     1201 gcgtggtgcc ggtggtggta gccgagacgc agcccgtcat tctggatgcc agcgcccagg
     1261 cagaaattgc ccgcaccatg ctgagccaga acctgaccat ccaactgcct ggtggcgaga
     1321 cggatgccgg gccctgggtt tttgagccgg ccactctggc cgaaatgctg acaattgagc
     1381 gggtggaaac cgagacggga gcccagtatc aggtggcgct ggacagcgaa gcgctgatta
     1441 ccttcctggc gaatattcag acggcggttg cccgcagccc ggagaacgcc cggtttatct
     1501 tcaacgacga cacccggctg ctggaagtga ttcagccggc ggtgattggc cgcagcctga
     1561 tggtcgatga gagtctggca gccatccaac agcaaaccac agccgggcag cacacggttg
     1621 aattgatgat tgattacatc aacccgacga ttaccaacga aaccaccggc gcagaactgg
     1681 ggattaccga gctggtcagt tcgcagacca gctatttccg gggttccagc cgggaccggg
     1741 tgaacaatat ccagattgcc tcggcgcagt tccacgggct gctgattccc cccggcgcca
     1801 ccttctccat ggctgaggcg ctgggcgatg tcagcctgga caacggctac gccgaagcga
     1861 tgatcatcgt tggcgggcgc accgtgaaag gcgtgggcgg gggtgtttgc caggtgagca
     1921 ccaccctgtt ccgcacggcg ttcatggggg gctaccccat tgcggagcgg cacgcccatg
     1981 cttaccgggt gtattactac gagcaaaact cgggcggcta taatgatgcg ctggccgggc
     2041 tggatgccac cgtgtttgtg ccgctggttg acttccgctt caccaacgat accccctact
     2101 ggctgctgat ggagacgtac gtcaacccct ccaattacag cctgacctgg aagttctact
     2161 ccaccagcga cgggcgcagt gtggaatggc ataccaccgg gccgaccaat attgtcgaac
     2221 cgcccgagcc caaatacatc gaaaacccgg agttgagcga ggggcagatc aagcaggtgg
     2281 actgggaagc cgaaggggcc gatatttcgg tcacgcgcat tgtctggcgc aacggtgaaa
     2341 tttacttcca ggacagcgtg tacacgcact atcagccctg gcaggcggtg tatgagtacg
     2401 gtccggggac ggagatcccc acgccagagc ccacgcccga agaataacgg ggctaatcgg
     2461 gggttggcca gccggcctgc ccgcataacg gaacaaaggc aaccggcagg atgtcttcgc
     2521 tgactaagcc agcggtcgta ttttgccaga gctggagtac ctgattgaaa cgcggaccca
     2581 ccggcagcac cagccgcccg cccggtgcca attgattcag caaggcgggg ggaaccgccg
     2641 gcgcggcagc cgtgacaagg atgcccgcat agggtgcggc agccggccag cccagcgagc
     2701 cgtcaccgat ttggacctgg acattgacgc agccaagctc agccagaatc tgccgggcct
     2761 gctcagccag ggacacgtga cgctcaacag tgtaaactcg ggctgccagg cggctcaaaa
     2821 tggcggcctg atacccggag ccggtaccaa cttccagtac ggtctcgctg ccatccaact
     2881 ggagcaggtc ggtcatcagc gccacaatgt aaggctgtga gatggtctgc cccaggccaa
     2941 tgggcagggg gccatcagcg taggcgtatt cctgcgactc gggcgggatg aagcgatggc
     3001 gggggatctg gcgcagggcg gtcagcaggc gcggggattc gatgccccgc cgaataatct
     3061 gctgctgtac catacgttca cgtgcggccg aaaattcatc catgccttaa ttgtagcaag
     3121 ccagagcgcg gaatcaatag ggttaaagac gcgcgattgc tgacacccca acagggttat
     3181 gaattttcag gctgatttct ggctgtctgc caggaggcgg tgggcaatct ggcggtcaat
     3241 tttgcccatg gggaaatccg gcagttcggc aggcagcgcc cagcacagtt cactggcgac
     3301 ctgcggctgc ggctctccgg tgaccagggt gcaggcgaaa gcatgcagca ccactttgaa
     3361 atgtgtatag gcgtgccggt aaacccccag ttctgcgcca acggcaatct caacagccag
     3421 ttcttcggcg atttcgcggc gcaggccttc gggcagggtc tcacccggct ccagcttgcc
     3481 gccagggaat tcccacaggc cgcccagcag gccgcgggag gggcggcggg caatcagcac
     3541 acgatctgcg cgccagatga cggcggcagt aaccgtccaa agcggtacgg gcgggcgggc
     3601 agacatcacc gggcgctcgg cttgcagacc cagggctgcg gcctggcata aggcctgcag
     3661 cgggcaaacc aggcaggccg gtgcgcgggg ggtacaaatc tgcgaaccga ggtccatgag
     3721 agcctggtta taatcgccgg cgcggccggg gggtaaatgc tgctgggcca gtgcccagag
     3781 ctgccgttcg ccctcggggg agcgtgcagg cagagtcacg ttaaataccc gggccagcac
     3841 ccggcggata ttgccatcca gggcggcttc gtcctgaccg aaagccatcg aggcaatggc
     3901 agcggcggta tagcgaccca cgcccggcaa aagcgccaga tcggcagcgg tgcggggcat
     3961 ttgcccacca gcttcccaga cctgccgggc ggcgcgccac agattgcgcg cccggctgta
     4021 atagcccaga ccttcccaga gcgccagaac ctcctgttcg ctggcggcag ccagcgcttc
     4081 cagtgtgggg aagcgcgcca tccagcgctc aaaatagggg ctgaccgtgt ccacctgggt
     4141 ttgctgcagc atgatctcag agacccagac cgcataaggg tctggatggc cgcgccaggg
     4201 cagagtccgg gcgttcacgg cgtaccaggc cagcagtttt tcggccagtt cagcgctcaa
     4261 agcagcctaa aattgaaacc cgttgcgggc gttgaccagc ttggctgagt aattgagaac
     4321 gtaatcttgc agttggttgt gcagattgac ccactcggtt gagcgcatga cctgccgggc
     4381 tttggaaagc gttggcagca caacccccac cataatctgc ggctgtgagc cgtaaaccgt
     4441 agcccaggca tcgctcaact caaaacccag ccgctgcaca ccggggatga agttactggt
     4501 cacaaactca aaatattcct gttcgtgatc gggggaaata tcccagctca taatcagttt
     4561 taccggcatg gccatactcc gcttacacgt tctgattttg attctgatat tccaggacaa
     4621 tcacctgggt ttcatctggc agggcgctga ggctggtgac cttgtaggat ggctgcggct
     4681 gcacactgct caggcccatc tctttgagaa atttgctcag gggttccatc tgggcggtgg
     4741 tttcggggat gtggtagtgc atggggatga cgatgttggg ctccagcagg ctgatcatct
     4801 cggcggcttt ggccgcggtg aggctgccgc ccccgcccac cggcaccagg gccacatgca
     4861 cggggcccag ggcttcaact tcggtttggg aaggcacccg gttcagatcc cccaggtgga
     4921 ccaccgtgat gccgttgtaa tcgagcagat aaagggtgtt gcgcggctcg tcttcggtct
     4981 ttttggtatg gccgttggtc tgaatgccag tgatgaagac accgccgatt tcaaactcgc
     5041 cggggccggt aatctggtag gggtcgcctt tgaccgccga gaggcagttg tgcccagggg
     5101 aggcatggct gaccgtgacc acatcggcct tgagcttgag gggagcatag cccacctgct
     5161 tggaatcata ggggtcggtg accactgtcg ccagactgcg ctcagtgagc cgaaaacacg
     5221 agtgaccata ccaggtaatt tccatacagc ctccatgcaa aatagcccgt ttgcggggcg
     5281 caacgctctg attatacacc agagcagggc aggaaggaaa agcgcgccca aaaacgatga
     5341 aatgcttgac gcgcgttggc agcagtgata gactcggcgg gcagcaatgg gaagcaaaat
     5401 accagactga gtcttgcgct tggcgggctg ctctggtata atctaccccg ctgtgtgttt
     5461 caggaagcaa tttaacaatt ttgcgccgac gtagctcaat cggcagagca accgccttgt
     5521 aagtggtagg ttatgggttc gattcccatc gtcggctcag ttgtcggttg gcgagcaagg
     5581 cggctctgca gagaagcttg ctggcgagcc gataacgctc ggtcaaatgg acggttaccc
     5641 aagtggccaa cggggactga ctgtaaatca gttgggcaac gcccttcgga ggttcgaatc
     5701 cttcaccgtc cactggtcgt ttgaccaact cgcccttacc acggcgagtc cgtctggcag
     5761 aacggcacgc cctcatagct cagatggtag agcacgccct tggtaagggc ggggtcatgg
     5821 gttcaaatcc cattgagggc tctcaatgcc ggaaagcgtg tcagcttcct gtaagctgca
     5881 agacctcgaa agaggaaaac acctgttcaa ggagaaggaa ttatggcgaa gcagaagttt
     5941 gatcgatcga agccgcacct gaacgtggga acgatgg
//
LOCUS       DAOPVS010000111         5915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_13391_length_5915_cov_57.245904, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000111 DAOPVS010000000
VERSION     DAOPVS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_5915_cov_57.245904"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKL11_08535"
     CDS             <1..415
                     /locus_tag="PKL11_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNZ13686.1"
                     /translation="NLLTSLQKSGLRLTATRVAICNLLAETTSHPTAAQIYDQLKPQY
                     PSLSLATVYNTLDVLVNMGVINVLGHSGDGNVHFEPNTSPHINLACVRCHKIQDLPAD
                     DLSRMEARVSTESGYKLLGARVMYYGICPECQAEH"
     gene            491..1204
                     /locus_tag="PKL11_08540"
     CDS             491..1204
                     /locus_tag="PKL11_08540"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ13687.1"
                     /translation="MENTPVQTTVMPRIGEKAPEFTAVTTQGPINFPADYQGSWVILF
                     SHPADFTPVCTSEFMTFASMEDQFAQVNTKLVGLSVDGLYSHIAWLRTIKEKIEYRGM
                     KDVEVKFPLIEDITMEVAKKYGMIQPGESSTKAVRAVFVIDPKGIIRTIIYYPLSLGR
                     NFQELYRVIVALQTADAFSIATPADWQPGDDVIVPTAGSCGVAKERMDNKQDMHCYDW
                     FFCTKKLDRETIFKTILKK"
     gene            1364..3430
                     /locus_tag="PKL11_08545"
     CDS             1364..3430
                     /locus_tag="PKL11_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ13688.1"
                     /translation="MNKLRWLFVGAMLLSLVFASLSPQPALGQARLTGTGNDVLRLSQ
                     YPVDTLDPSLASFVQEVQVINSLFAGLVKLDLATGQVVPDLAESWSWNAEKTEVTFTL
                     RTGLVWSDGAALTASDARYAILREIDPEMYASYAAILDPIVNAYEYRTGQITDPNLVG
                     VIVDDATHLRIQLTQPMVFFDKIMALWVTKPLPQHVIALHPDNWTDPAYIVTSGPYTL
                     TEWDQASYLHLAANPNYYGTPPGYSEIAVALTDNDQQRWDAYLQGNLDTFDVTAAQYQ
                     QAQADPILAGQTALTPGTCTYALVFKTDTPPMDNANVRKALIAAIDRTDFAAQFAGDA
                     NLATQTWTPPLIWGYVDGPALGIGIPFDVTQAQAYLTAAGYPNGAGFPAVAVEVRDTS
                     RNRLRWDYMAAAWQANLNITISVTYIGTNPPAQPINFTGWCGDYNEAFNFVGDGIQYL
                     NAAGRLGGWSNAAYDALIDQISQEADLPTRLGLYEDVEQILVEDEAVIGPLHNTMRAT
                     LTKPYLQRTISGLSQDYWYEWSLIMEAEANYDPGNTMNFEPGVDTTAYQLPAAAFTQA
                     MVLTHTAIRPDELAIQPPDGLGGIGHAYDLSAVPQTLGNTLNDQTNLPYLVIIPYSPE
                     QVAAGDVAEETLGLYALVEGVWINQGSSVNTETHELTAATWEINTDFAVFGVTYKHIY
                     LPGILR"
     gene            complement(3486..>4085)
                     /locus_tag="PKL11_08550"
     CDS             complement(3486..>4085)
                     /locus_tag="PKL11_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13689.1"
                     /translation="PGAHPMKTPGTIIFLNGTSSSGKTCLLEALQERLEPPYLNMGID
                     KFIWMLPERCLERPLWDDVLGLADHAGQSGHRLMTTMHHAIAAAARRGSHILADHVLV
                     EPRWVTECARLFHALPAWLIGVRCELSVLEERERARKNRTLGQARAQYDCVHAHGIYD
                     FEVDTAHFSTAECADQIMAHLQSGARPAAFQQLYEKPYR"
     assembly_gap    4086..4185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4186..5899
                     /locus_tag="PKL11_08555"
     CDS             <4186..5899
                     /locus_tag="PKL11_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNZ13690.1"
                     /translation="GGEAMSHKFSRVVWLVMALLLAACTPNGGVEPTAPPLASPSAGP
                     GTPDAPQPTPTLGLPAGPRAAIVELINQVEAHPLPESGWQPAAVAMTIYTGGEVWAKE
                     NATARVQLDESVIRVAPNTIFSLKPAADQTVTLDLAQGQVWLNVEGLAPGETLEVQTP
                     DAVAAVRGTRFSVRVLAEGGTVVSTREGAVAVTAAGSTVTVDAGMQSTILPGGAPGQP
                     APLSIPEQGLWGMAADPHLDVALPVARELTSLTGPGIVMNPAWSPDNQLVFQHYDPAQ
                     PDKASRWDALDPATGQTIVTSLYGELDGLRFNASGQVAYLSYQGGSNRICTRPALTSP
                     TEDCFTPDVSGAGWPFWSPDGQWILFYAGGGSVMNLYRSRPDGSDLLQLTRWATGAAH
                     RQTWSPDSQQVAYVYAPQYGQDGQLWVVNRDGTASPNLPLLENVFGDGQHVAWSPDGA
                     LLAAPVAQDGLWLVQPDGQNARRLAATAAAGEYRSVEWSQTATGWPLWYRYRASEADP
                     WVKYALLSETAQPFALGEDLWGPIWSPDGSRAALGSNLKTDPAANQYEYHLTVLSLEP
                     DFWP"
BASE COUNT         1132 a         1841 c         1728 g         1114 t
ORIGIN      
        1 aaacctgctc acctcactcc aaaaatccgg cctgcgcctg acggccaccc gcgtggccat
       61 ttgcaacctg ctggccgaaa ccaccagcca ccccaccgcc gcgcagatct acgaccagtt
      121 gaagccgcag tacccttcgc tctcgctggc tacggtttac aacacgctgg atgtgctggt
      181 gaacatgggt gtcattaatg tgctgggaca ttcgggcgat ggcaatgtcc attttgagcc
      241 caacaccagc ccgcacatta atctggcgtg cgttcggtgc cataaaatcc aggacctgcc
      301 tgccgatgac ctcagccgca tggaagcccg cgtcagcaca gaatcgggct ataagctgct
      361 gggtgcacgg gtgatgtatt acggcatctg cccggagtgc caggcggaac attaacccgt
      421 caacacacct gccgcccggc aggttgaaca aaccgcaacc aaccctattc agttatgaag
      481 gagataaaaa atggaaaaca cccctgttca aaccaccgtc atgccccgca ttggtgaaaa
      541 agcccccgaa tttaccgccg tcaccacgca gggtcccatc aatttccctg ctgattacca
      601 gggcagttgg gtgatcctct tcagtcaccc cgcagatttc acccccgttt gtacctctga
      661 atttatgacc tttgcctcga tggaagatca atttgcccag gtcaacacca aactggtcgg
      721 gctctcggtc gatggcctgt acagtcacat tgcctggctg cgcaccatca aagaaaaaat
      781 cgaataccgc ggcatgaagg atgttgaggt caagttcccg ctgattgaag acatcaccat
      841 ggaagttgcc aagaagtacg gcatgatcca gccgggtgaa agcagcacca aggccgtgcg
      901 cgccgtcttt gtgattgatc ccaagggcat cattcgcacc atcatctact acccgctcag
      961 cctggggcgc aacttccagg aactgtatcg ggtgattgtg gccctgcaaa ccgccgacgc
     1021 cttttccatt gccacccctg cagattggca gcccggcgat gatgtgattg tgcccacggc
     1081 gggttcctgc ggtgtggcca aagagcgcat ggataacaag caggatatgc actgctacga
     1141 ctggttcttc tgcaccaaga aactggaccg cgaaacgatc tttaagacca tcttgaaaaa
     1201 gtaatccgcc tgtcacggat caccttcacc ccggagcagc cttgggtctg ctccggtttt
     1261 tttattctcc cggcatccgg tgattgacaa accccagttt cgttctacaa tcattaatac
     1321 cgaagattta cttcgtccat tcccaattca aggagatgcc actgtgaata agctacgctg
     1381 gttattcgtg ggagccatgc tgctcagtct cgtgtttgcc agcctgtcgc cgcagccggc
     1441 cctggggcaa gcgcgcctca ccggcacagg caatgatgtg ctgcgcctgt cgcagtatcc
     1501 ggtggatacg cttgacccct ccctggcctc gtttgtgcag gaagttcagg tgatcaattc
     1561 gctgtttgcc ggtctggtca agctggatct ggcgaccggg caggtggtcc ccgacctggc
     1621 cgaaagctgg agctggaatg cggaaaaaac cgaggtcacc ttcaccctgc gcaccgggct
     1681 ggtgtggagc gatggcgccg ccctgaccgc cagtgacgca cgctacgcca ttctgcggga
     1741 gattgacccg gagatgtacg caagctatgc cgccatctta gacccgattg tcaacgccta
     1801 tgaataccgc acagggcaaa tcactgaccc gaatctggtg ggggtgattg ttgatgacgc
     1861 cacccatctg cgcatccagt tgacccagcc gatggtcttc tttgacaaga tcatggcgct
     1921 gtgggtgaca aagcccctgc cccaacatgt gattgccctg cacccggaca attggaccga
     1981 cccggcctat atcgtcacca gcgggcccta cacgctgacc gagtgggacc aggccagtta
     2041 tctgcacctg gcagccaacc ccaattacta cggcaccccg ccaggctaca gcgaaattgc
     2101 cgttgcgctt acagataacg atcaacagcg ctgggatgcc tacctgcagg gcaacctgga
     2161 cacgtttgat gtgaccgctg cgcagtatca acaggcgcag gccgatccga ttctggcggg
     2221 ccagacggcg cttacaccgg gcacctgcac ctatgccctg gtcttcaaga ccgatacgcc
     2281 ccccatggat aatgccaatg tgcgcaaagc gctgattgcc gccatagacc gaaccgactt
     2341 tgcagcccag tttgccgggg atgccaacct ggcaacccag acctggacgc cgccgttaat
     2401 ctggggctat gtggatgggc cagccctggg cattggcatt ccgtttgatg tgacccaggc
     2461 gcaggcctat ctgaccgccg ccgggtaccc caacggagcg ggcttcccgg cggtggctgt
     2521 ggaagtgcgg gatacgtctc gcaaccggct gcgctgggat tatatggcag cagcctggca
     2581 ggccaatctg aacatcacca tttcggtaac gtatattggc accaatccgc ctgctcaacc
     2641 gatcaatttt accggctggt gcggcgatta taacgaagcc tttaactttg ttggggatgg
     2701 cattcagtac ttaaatgcgg ctgggcgcct gggcggctgg tccaacgcgg cgtacgatgc
     2761 cctgattgac cagatttctc aggaagccga cctgcccacc cggctggggc tgtatgaaga
     2821 cgtggagcaa attctggtgg aagatgaggc cgtcattggc ccgctgcata acaccatgcg
     2881 cgccaccctc accaagccct acctgcagcg caccatctcg ggcttaagcc aggattactg
     2941 gtatgagtgg tcgctgatta tggaggcaga ggccaattat gaccccggca acaccatgaa
     3001 ctttgagccg ggcgtggata ccacggctta ccaactcccc gcggctgcgt tcacccaggc
     3061 catggtgctg acgcataccg ccatacgccc cgatgaactg gcgattcagc cgcctgatgg
     3121 tctgggcggc atcgggcacg cctacgacct gagcgctgtg ccgcaaaccc tgggcaacac
     3181 cctgaatgac cagacgaatt taccgtatct cgtcatcatc ccctacagcc ccgagcaggt
     3241 tgccgccggg gatgtggccg aagagaccct ggggctttat gccctggtgg agggcgtctg
     3301 gatcaaccag ggcagcagcg tcaataccga aacgcacgag ttgacagccg ccacttggga
     3361 aatcaatacc gattttgccg ttttcggcgt tacctataag cacatttacc tgccgggcat
     3421 tctgcgttag cctggctcgc ttaagatgaa aaactccccc ggtctggttg gccgggggag
     3481 tttgattatc ggtacggctt ttcgtaaagc tgctggaagg cagccggacg ggcgccgctt
     3541 tgcaggtgcg ccataatctg gtcggcgcat tcggcggtgc tgaaatgggc tgtgtccacc
     3601 tcaaaatcat agatgccgtg ggcgtgcaca caatcgtact gcgcccgggc ctgccccagg
     3661 gtgcggttct tgcgggcgcg ctcgcgttct tccagcacgc tcagctcgca gcgcacgccg
     3721 atcagccagg cgggcagcgc gtgaaacagc cgggcgcatt ccgttaccca gcgcggctcc
     3781 accagcacat ggtctgccag gatatggctg ccccgccgcg ccgcggcggc aatggcatgg
     3841 tgcatggtcg tcatcaaccg gtgaccgctc tgcccggcgt ggtctgccag ccccagcaca
     3901 tcatcccaca gcgggcgttc caggcagcgc tcgggcagca tccagatgaa cttgtcaatg
     3961 cccatgttca aataaggcgg ctccaggcgc tcctgcaggg cctcgagcag gcaggttttg
     4021 ccggaactgg acgtgccgtt gagaaaaatg atggtgccgg gggttttcat cgggtgtgct
     4081 ccaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggag gtgaagcgat
     4201 gagccataaa ttttcccggg tggtttggct tgtgatggcc ctgctgctgg cagcctgcac
     4261 ccccaacggc ggggttgaac ccaccgcgcc gcccctggcc tcgccctctg ccgggccagg
     4321 caccccggac gcgccgcagc ccacccccac cctgggactg cccgccgggc cgcgcgccgc
     4381 cattgtcgaa ctgatcaacc aggtggaggc ccaccccctg ccggagagcg gctggcagcc
     4441 cgccgccgtg gcgatgacca tctacaccgg cggcgaggtc tgggccaaag agaacgccac
     4501 ggcccgggtt caactggatg agagcgtgat ccgggtggcg cccaatacca tcttctcgct
     4561 caagcccgcc gccgaccaga ccgtgacgct ggacctggcg caggggcagg tctggctgaa
     4621 tgtggaaggg ctggcgcccg gcgagacgct ggaggtgcaa acccccgatg ccgtggcggc
     4681 ggtgcgcggc acacgcttca gtgtgcgcgt tctggcagag ggcggcacgg tggtctccac
     4741 ccgtgagggg gcggtggcgg tcacggctgc cggcagcacc gtgacggtgg atgccgggat
     4801 gcagtccacc attctgccgg gcggcgcccc cggccagccc gccccgttga gcatccccga
     4861 gcaggggctg tggggcatgg ccgctgaccc tcatttggat gtggctttac ccgtcgcccg
     4921 tgagctgacc tctctgaccg gcccgggaat tgtgatgaac cccgcctggt cgccggacaa
     4981 tcaactggtc ttccagcatt atgaccccgc ccagccggat aaagcgtccc gttgggatgc
     5041 gctggaccct gcaaccgggc agacgattgt gaccagcctg tacggcgagc tggatgggct
     5101 gcgttttaac gccagcgggc aggtggctta cctgtcttac cagggcggct caaaccgcat
     5161 ctgcacccgc ccggcgctca ccagccccac cgaagactgc ttcacccccg atgtcagcgg
     5221 ggcgggctgg ccgttctggt cgcccgacgg gcagtggatt ttattctacg cgggcggcgg
     5281 cagtgtcatg aatctctacc gctcgcgccc cgatggctca gacctcttgc agctcacccg
     5341 ctgggccaca ggcgccgcgc accgccagac ctggtcgcca gacagccagc aggtggccta
     5401 tgtctatgct ccgcagtacg gccaggatgg gcagctctgg gtggtcaacc gggatggcac
     5461 cgccagcccc aatctgcccc tgctggaaaa cgtctttggc gatggtcagc atgtggcctg
     5521 gagccccgat ggcgcgctgc tggccgcacc ggtagcccag gatggcctct ggctggtgca
     5581 gcccgacggg cagaacgccc ggaggctggc agccaccgcc gcggcggggg aataccgctc
     5641 ggtggaatgg tcgcaaacgg ccacgggctg gccgctgtgg taccgctacc gcgccagcga
     5701 ggccgatccg tgggtcaagt atgcgctgct ttcagaaacg gcccagccct ttgcgctggg
     5761 agaggatctg tgggggccca tctggtcgcc ggatggcagc cgggcggcgc tgggcagcaa
     5821 cctcaaaacc gacccggcgg ccaatcaata tgaatatcat ctgaccgtgc tgagccttga
     5881 gccggatttt tggccgtagg agagcgtttt aagtt
//
LOCUS       DAOPVS010000112         5847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_13603_length_5847_cov_55.020891, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000112 DAOPVS010000000
VERSION     DAOPVS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13603_length_5847_cov_55.020891"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKL11_08560"
     CDS             <1..686
                     /locus_tag="PKL11_08560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HNZ13691.1"
                     /translation="SQAAQRVVAVELDQQLVPILQQVLAGMTNVQIVAGDILEQNPAE
                     LMQSDQYLVVANIPYYITSAIIRHLLAASQKPRRLVLTIQREVAERICAAPGDMSLLA
                     LSVQVYGAPRVAARIPAGAFYPAPDVDSSVLRVDLYAEPRIPEAQLGEFFTLARMGFS
                     QKRKTLRNTLAAGLGCKGDAAAEMLHAAGIDPQRRAETLNLDEWRQVTDAIGPQLKQL
                     RKPVEPHPS"
     gene            complement(646..1260)
                     /locus_tag="PKL11_08565"
     CDS             complement(646..1260)
                     /locus_tag="PKL11_08565"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ13692.1"
                     /translation="MTKIFELPALKFAFDALEPAIDARTMEIHHGKHHAAYVANLNKA
                     LESAPEYYEHTITDILRNIQSVPEAIRTAVRNNGGGHANHTLFWDILQPGGSKLPHAS
                     LAKMIDSTFGSYDAFVEKFNQAALTRFGSGWAWLALDAQKKLYVYSTANQDSPLMDGH
                     TPILGLDVWEHAYYLNYQNRRADYIKAFWGLINWDVVQQAYEAA"
     gene            1490..2503
                     /locus_tag="PKL11_08570"
     CDS             1490..2503
                     /locus_tag="PKL11_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ13693.1"
                     /translation="MSITIRDIAQKLNLSIAAVSRALDGYPDISPETRERVIRAAEEM
                     GYAPNRAARQLRRQKSDTIGYIMPVYSPRFADSYYSEFIEGMGDESAKQGFDLLISTA
                     PPQSKTEQQIYHQWVQGNKVDGLVLNRICVHDWRVQYLQDQHKPFSGLEHSLDGVDYP
                     RVEVDNFNGFVKLVEHVLQYGFERIAYIGGPGALKIQADRLAGYQSGLAAAGMYQDSD
                     YVCSGDLTSEGGYAATQVLLSLPKPPNAILCVNDQTAFGALHAANEADLRIGEQIAIA
                     GFDGVRDARYSNPPLTTVDQPVYEIARQLVQMLAVEIRGEPMPARSVLIEPKLLLRAS
                     TGA"
     assembly_gap    2628..2727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2728..3699
                     /locus_tag="PKL11_08575"
     CDS             <2728..3699
                     /locus_tag="PKL11_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNZ13694.1"
                     /translation="ASRTGNYPLLRTYSGTDDFLQLAEVYVETINIAWCAIPLFWFNQ
                     MDGRGPWGLGGSIEEHQKVMRWYGTRGIPVELNEPHHWGMRDASDVIFVVSAYLAAYN
                     ARAFGVKDYIAQLMFNSPPGLSDAMDLAKMLAILELIEPLSGPDFRILREVRTGLLSY
                     PLEPAAARAHLAASIYVQMALKPQIVHIVGHTEADHAATADDVIEAAYMAQRVIENAL
                     KGAPDLTADPAVQQRKQQLIEEAQITLEAIRRLGPESQDPLIDQVVLEKAVTEGIMDA
                     PQLKNNPFGKGILKTRIVNGACMAIGPDDQPIGEAKRLGKFLKEKDL"
     gene            3696..4805
                     /locus_tag="PKL11_08580"
     CDS             3696..4805
                     /locus_tag="PKL11_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNZ13695.1"
                     /translation="MNIPNNFTVIGAGHGGKAMAAHLALMGRRVVLYNRTYSHIEIVG
                     RRGGIELESYEGGPRGFGQLAGVTSDIEEAVKDAEVIMVVVPSSAHADIARKVAPFLK
                     DGQIIILHPGRTCGAIEFDAVLREMDCQANVILAEAETFIYASRSDGPAQARIFRIKE
                     AVPLAALPANQTKNVLKAIEEVYPQFIDGVNVLNTGLNNMGAIFHPALTILNAGRIEN
                     THGDFQFYIDGVTPSVARVLEALDRERVTVASSLGIRARTAMEWLELAYNVTGEDLDQ
                     AIHNQPGYYGIKAPNTLYHRYIFEDVPMSLVPIASLGQRYGVSVNGMDSLIRMASIIH
                     KTDYWRRGRTLSKLGLKDLSIGEITEYVNTGTRDF"
     gene            4827..5405
                     /locus_tag="PKL11_08585"
     CDS             4827..5405
                     /locus_tag="PKL11_08585"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNZ13696.1"
                     /translation="MNIPFLADIALPDRLWLPLLIFFARVLDVSLGTLRIIFISRGKK
                     YLAPALGFLEVFIWIVAVSQIMRGVQDWVSYLAYAAGFATGNYVGMWIENRLAIGTLI
                     IRTILPNGADELVGRLRQAKYGVTSVQGQGSNGPVTLVYTIIKRKDLGAVVGIIHQLF
                     PHAFLTVEELRSTAEGIFPPEQTMLQTMVKKK"
     gene            5444..>5847
                     /locus_tag="PKL11_08590"
     CDS             5444..>5847
                     /locus_tag="PKL11_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNZ13697.1"
                     /translation="MCGIAGSYPVSQPEIVNKMIEKIRHRGPDSHAVIEAPNGALGHT
                     RLAILDLDGGAQPMAFGDYQIAFNGEIYNHQALRAQYLSNAPLKTHSDTEVLLHLYAQ
                     LGPEMISRLDGMFAIAILKDDALFLARDPIGL"
BASE COUNT         1311 a         1356 c         1759 g         1321 t
ORIGIN      
        1 tgagccaggc cgcgcagcgg gtggtggcgg ttgagttgga tcagcagttg gtgcccattt
       61 tgcagcaggt gctggccggc atgaccaatg tacaaattgt ggcgggggat attctggagc
      121 agaatccggc agagctgatg cagtcagatc aatacctggt cgtggcgaac atcccttact
      181 acatcacgtc ggcaatcatc cggcacttgc tggcagcgag ccaaaagcca cggcggctgg
      241 tgctgacgat tcagcgcgag gtggcggaac ggatttgcgc ggcgccgggt gatatgagcc
      301 tgctggcact cagcgtgcag gtttatgggg cgccacgggt tgcagcccgg ataccagcgg
      361 gcgcatttta tccggcgccg gatgtggatt caagtgtgct gcgggtagat ttatatgcag
      421 agcccagaat cccggaagcg cagttggggg agttcttcac actggcccgg atgggattca
      481 gccagaagcg caagacgctg cgcaacactc tggcggcagg gctggggtgc aagggcgacg
      541 cggcggccga aatgctgcac gccgccggga ttgacccaca gcggcgtgct gaaacattga
      601 atttagatga gtggcgtcag gtaaccgacg ccattgggcc gcagcttaag cagcttcgta
      661 agcctgttga accacatccc agttaatcag accccagaat gctttgatgt aatcggcgcg
      721 gcggttctga taattgaggt agtaggcgtg ttcccagaca tccaggccaa ggatgggggt
      781 atgaccatcc atcagagggc tgtcttgatt ggcggttgaa tagacgtaca gctttttctg
      841 agcatccagg gcgagccagg cccagccaga gccaaagcgt gtgagggctg cctggttgaa
      901 tttctcaaca aaggcatcgt aagagccaaa ggttgagtcg atcatcttgg cgagggaggc
      961 gtgaggcagt ttggacccac ccggctgaag gatatcccag aataaggtgt ggttggcatg
     1021 gcccccaccg ttgttacgca cggcggtgcg gatcgcctca gggacgctct gaatgttgcg
     1081 gagaatatcg gtgatggtgt gttcgtaata ttcgggggcg ctttcgagcg ctttattgag
     1141 gttggcgaca taggcagcgt gatgcttgcc atgatgaatt tccatagtgc gggcatcaat
     1201 ggcgggttca agcgcatcga aggcgaattt gagagcaggg agttcaaaga ttttagtcat
     1261 cgggcaattc ctttcaggaa gtgattgatt ttatttacga tattattgat gataataatc
     1321 aaaaatagtt acattgtcaa actggctgtt acaggttagg tcaaccctgg ggtagcttag
     1381 ggagttctaa aactggcacc ctggacaggt cataaattga gtcttgcagg gagaaaaatt
     1441 ttccggtaaa atgattttac tgaaacgttt aagtaaagtg agaggacaga tgtcaatcac
     1501 catccgtgat attgctcaaa agttgaattt atccattgcg gcggtttcgc gcgccctgga
     1561 tgggtatccg gatatttctc cagagacacg ggaacgggtt atccgggcgg ctgaagagat
     1621 gggctacgcc cccaaccggg ccgcgcgtca actgcggcgg cagaagagcg acacgattgg
     1681 gtatattatg ccggtgtact caccgcgttt tgcagattcc tactattcgg aatttattga
     1741 gggcatgggg gacgaatcgg caaagcaggg ttttgatcta ctcatcagca ccgccccacc
     1801 tcagagcaag accgaacagc agatctatca tcaatgggta cagggcaata aagtggatgg
     1861 gctggtgttg aaccggattt gtgttcacga ctggcgcgtg caatatctgc aggatcagca
     1921 caagccgttc agcgggttag aacattcgct tgatggggtt gattacccac gggttgaagt
     1981 tgacaacttt aatgggtttg tgaagctggt tgaacatgtg ctgcaatatg gatttgagcg
     2041 gattgcctac atcggtgggc cgggtgcgct caaaattcaa gctgaccgac tggctggata
     2101 tcagtccggg ctggcagccg cgggaatgta ccaggactcc gactatgttt gctcgggaga
     2161 tttgaccagc gagggaggat acgcggccac acaggtcctg ttatccctgc ccaagccccc
     2221 aaatgccatc ttatgtgtta atgaccaaac cgcttttggt gcgctgcacg cggccaacga
     2281 agcggacctg cgaattggcg agcaaattgc cattgccggg tttgacgggg tgcgcgatgc
     2341 gcgctacagc aatccgccgc tcacaacggt tgatcaacct gtctatgaga ttgcccggca
     2401 actggtgcaa atgctggctg tagaaattcg cggggaaccg atgcctgcgc gcagtgtgct
     2461 gattgagccc aaactgctcc tgcgcgcttc caccggagcg taagtttttt gcgctctgtt
     2521 tgcaagaaag gagagcgacg aatgaaaacc gtcattgcgg cagccctggg cgaatgcgtc
     2581 catgtggcag gaatcagtaa ttttttacgc ctggctgaac aggccggnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc agccgcacgg ggaattatcc actgctgcga
     2761 acctattcgg gcacggatga ttttctacaa ctggccgaag tgtacgttga gacgattaac
     2821 atcgcctggt gtgccattcc cctgttctgg ttcaaccaga tggatgggcg cggcccatgg
     2881 ggattgggcg ggtcgattga ggaacatcag aaagtgatgc gctggtacgg cacacgcggc
     2941 attccggttg agctgaacga gccgcatcac tggggcatgc gggatgcctc tgatgtgata
     3001 tttgtggttt cggcctatct ggcagcttac aatgcccggg ctttcggcgt gaaagattat
     3061 attgcgcagc ttatgttcaa cagtccgccg ggactgtcgg atgcgatgga cctggcaaag
     3121 atgctggcaa ttctagaact gattgagccg ctcagcgggc cagatttccg catcctgcgc
     3181 gaggtgcgca cgggactgct cagttacccg ctggagccag cggcagcgcg ggcccatctg
     3241 gcggcgagca tttacgttca aatggccctg aagccgcaga ttgtgcatat tgtggggcat
     3301 actgaggctg accatgcagc aacggcggat gatgtgattg aggcggccta tatggctcag
     3361 cgcgtgattg aaaacgcgtt gaagggagca ccggacctga ctgccgatcc ggctgttcaa
     3421 cagcgaaagc agcagttgat agaagaggct cagatcacgc tggaggccat ccggcggctg
     3481 gggcctgaga gtcaggaccc cttgattgat caggtggttc tggaaaaagc ggtgactgag
     3541 ggcattatgg atgccccaca gctcaaaaat aacccctttg gaaagggcat acttaagacc
     3601 cggattgtca acggcgcctg tatggcaatt ggtccggacg atcagccgat tggtgaagcc
     3661 aaaaggctgg gcaagttttt gaaggagaaa gacttatgaa cattccaaat aactttactg
     3721 taattggcgc cgggcatggc ggcaaggcaa tggctgcgca tctggcgttg atgggccggc
     3781 gggtggtgct gtataaccgc acctacagcc atattgagat tgtgggacgc cgcggcggca
     3841 ttgaactgga gagttatgaa ggcgggccgc ggggctttgg gcagcttgcg ggagtcacat
     3901 ctgacattga agaggctgtt aaagatgccg aagtcatcat ggtggttgtg ccatcttctg
     3961 cacatgccga tattgcccgc aaagtggcac ctttcttgaa agacggccag atcattatcc
     4021 tgcatccggg gcgcacctgc ggcgcgattg aatttgacgc ggtgctgcgc gagatggact
     4081 gccaggctaa tgttattctg gcggaagccg agacgtttat ctatgccagc cggtctgatg
     4141 gtccagccca ggcgcgcatc tttcgcataa aagaagcggt gcccctggcg gcgctgccgg
     4201 ccaatcagac taagaatgtg ctcaaggcga ttgaagaggt gtatccgcag tttattgacg
     4261 gcgtgaatgt tttgaatacg gggctgaaca atatgggcgc catcttccat ccggcgctga
     4321 ccattctcaa tgcgggacga attgaaaata cgcatggcga ttttcaattt tacattgatg
     4381 gcgtgacgcc ttcagtggcc cgggtgctgg aagcgttgga tcgggaacgg gtgacggtcg
     4441 cctcatcgct ggggattcgc gcccggacgg cgatggaatg gctggagctg gcctacaatg
     4501 ttaccgggga agacctggat caggctattc ataatcagcc gggatattat ggcatcaagg
     4561 cgcccaacac gctgtatcac cggtacattt ttgaggatgt gccaatgagt ctggtgccca
     4621 tcgcttcgct ggggcagcgc tatggggttt cggtgaatgg gatggacagc ctgattcgga
     4681 tggcgagcat cattcataaa accgattact ggcggcgcgg ccggacattg tccaagctgg
     4741 ggcttaaaga cctgagcatt ggcgagatta ccgaatatgt taatactgga acgcgcgatt
     4801 tctaagcaga tgacgaggta cctacggtga atattccctt ccttgctgat attgccctgc
     4861 cagaccgatt gtggctgccg ctgttgattt tctttgcgcg ggtgctggat gtttcgctgg
     4921 gaaccctgcg gatcatattc atctcacgcg gcaagaaata tctggcgccg gcgttgggtt
     4981 ttctggaagt gtttatctgg attgtggcgg tcagtcagat tatgcgcggc gtgcaagatt
     5041 gggtttctta cctggcttat gccgccgggt ttgccacggg caactatgtg ggcatgtgga
     5101 ttgaaaaccg actggcgatt gggacattga tcatccgcac cattctacct aatggtgcgg
     5161 atgaactggt ggggcgcctg cgtcaggcta aatacggcgt gaccagcgtt cagggccagg
     5221 gcagcaatgg accggttacg ctggtgtaca ccattatcaa acgaaaagac ctgggggcgg
     5281 tggtgggaat tatccaccag ttgtttcccc atgcattttt gactgtggaa gagctgcgat
     5341 cgacggcgga gggcattttc ccaccggaac aaaccatgct gcagaccatg gtcaaaaaga
     5401 aataagctgc accattgtgc agaattggag aaaggaaaac cttatgtgcg gaattgctgg
     5461 atcttatcca gtctctcaac ctgaaattgt gaacaagatg attgaaaaaa ttcgacaccg
     5521 cggccccgac agccatgcag tgattgaagc accaaacggt gctttagggc atacccggct
     5581 ggcaattcta gatctggatg gcggcgccca gccaatggct tttggagact accagattgc
     5641 gttcaatgga gaaatctata atcatcaggc tttgcgagcc cagtatttgt caaatgcccc
     5701 tctgaaaact cattctgata cagaggtact gttacatctc tatgcccagt taggaccaga
     5761 gatgatctcg cgactggatg ggatgtttgc aattgcaata ctcaaagatg atgcgctatt
     5821 cctggcgcgc gatcccatcg gactaaa
//
LOCUS       DAOPVS010000113         5808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_13728_length_5808_cov_57.399965, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000113 DAOPVS010000000
VERSION     DAOPVS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13728_length_5808_cov_57.399965"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKL11_08595"
     CDS             <1..311
                     /locus_tag="PKL11_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13698.1"
                     /translation="PAAFEEALLNNWGRQVDPQVLLTALKLRNVMPEGAELAYSQRHG
                     LGWEEATGTFVYIGHSLDEIDLKLVEAQAILQELANQGHTPTVVSVETVHAPYYRMEQ
                     "
     gene            315..1547
                     /gene="ftsA"
                     /locus_tag="PKL11_08600"
     CDS             315..1547
                     /gene="ftsA"
                     /locus_tag="PKL11_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNZ13699.1"
                     /translation="MEDLIVVGIDIGTTKTCTLVARVEGERSLRILGVGIEPSQGIKK
                     GVIVDLTAASQSIARSIEKAQRSSGLEITSALVSLAGSHVASVNSHGVVGISGRAIDE
                     EDVARALEAAQAVAIPHNREIIHVIQRGFMIDGQGGIRAPLGMHGYRLEVEAHIITAA
                     ESTVENLRQCVNAAGVEVSQFVLNPLASAEVVLSETERQMGVVVCDIGGGTTDLAIYI
                     DGDVWHTSVLPVGGNHITSDIAHGLRLPISQAEDVKKQYGHALEAEVGADETFTIRPF
                     GEETPIQFSRRELATIVGARTEEIFDMALQEIKRSGYDGLLPAGVVLTGGASQLPGLR
                     AMASQVLALPVRLARPENLLGLVDQLNSPAYSTSVGLLYWALLMSETAPVPTGRRPQK
                     GVPLDWDTIKNYLKRLLP"
     gene            1594..2766
                     /gene="ftsZ"
                     /locus_tag="PKL11_08605"
     CDS             1594..2766
                     /gene="ftsZ"
                     /locus_tag="PKL11_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNZ13700.1"
                     /translation="MDLTNQPPVESFARIKVIGVGGGGCNAVNRMIDEGLSGIEFVAV
                     NTDAQALMLAKAGTRVRVGDKSTRGLGAGGNPEMGRKAAEESAEELYEVLRGSDMVFV
                     TAGLGGGTGTGAAPIVAQIAKEVGALTIGVVTRPFTFEGSRRASSAEDGINRLKEHAD
                     TLIVIPNDRLLQIVDKRSSLQDAFRVADDVLRQGIQGISELITVPGLINLDFADVKAI
                     MSEGGAALMAVGHATGEDKARVAAEMAISSQLLDITIDGARGILFNVTGGPNLTLFDV
                     NQAAAIIKETAHPDVNLIFGAVIDQNMGDEIRITVIATGFDRTGMRLPTPQEMRAARP
                     ATPQPISAQPAQTTNGARQPVEVHSSAPAPFPPQVVNTGDLDVPAFLRKRSDSQPR"
     gene            2842..>3354
                     /locus_tag="PKL11_08610"
     CDS             2842..>3354
                     /locus_tag="PKL11_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13701.1"
                     /translation="MSRIRTLLWWILCAAISTMLLVAFVRFSGVNHNEKAPLAAMLNG
                     QAYRPYVTRALVPGLVNGLTGLLPPATQQVLNERLNGTYATARLLAMFSASPELALQG
                     MLTLAVMWASLLGFGAALRALMSAVYDAPPLTIDVLSLLALCGLLPFFGFGYIYDFTV
                     LWLFTLALALM"
     assembly_gap    3355..3454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3455..3769
                     /locus_tag="PKL11_08615"
     CDS             <3455..3769
                     /locus_tag="PKL11_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13702.1"
                     /translation="EFHLGDHAFMLQNFPVLGGLTTLIGVGLLALMALRPRQKPPFLL
                     AAAVIFVPLLALYIPFGFPYEIRVFYEGYAAGTLLVMQTLLGRRAWGLAPKPEPNPNA
                     IL"
     gene            4110..4568
                     /gene="nrdR"
                     /locus_tag="PKL11_08620"
     CDS             4110..4568
                     /gene="nrdR"
                     /locus_tag="PKL11_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNZ13703.1"
                     /translation="MRCPYCQNEESRVLDTTHDTRGGVRRRRECDQCHQRFSTYERPI
                     LATPLLVKRDGTREEFDREKLMRGIRISCAKRPVSAAEIDRLVGEIESALQQMGRAEV
                     SARVVGDMVIAGLKKLDQIAYIRYAIVYLHLDDLQAIRTEIDRLLEGDHS"
     gene            4565..>5808
                     /locus_tag="PKL11_08625"
     CDS             4565..>5808
                     /locus_tag="PKL11_08625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNZ13704.1"
                     /translation="MTDTAPTRAADWTTTPTMPEDLPPVNLTENARQVLVRRYVRRGS
                     NGEPAETVEEMFWRVAYNIAIVEKAWGTDVMALAREYYELLTSKSFFPNSPTFTGAGT
                     PLGQLAACFVLPISDDMGRASTGIFQTLRDAALIQQTGGGNGFSFSRLRPKGSLVISS
                     AGQATGPVGFLRVYDHAFGEVAQGGSRRGANMAVLRVDHPDIEEFIACKTDENQITNF
                     NISVGITDAFMRAVEADEDWELRFPDVHDARYKGFDGTLEQAEAAGIPILVHKRVRAR
                     ELFDKIVKQAHHNGEPGMLFLDAANRGNPVPHLYQLEATNPCGEQWLGGYENCCLGSV
                     NLAQHLGEQGTVDWEKLRRNVELATRFLDDVVDANHYVPAVPALKEAAHRARRIGLGI
                     MGLADLMYHVGVRYGSPEGQEFA"
BASE COUNT         1122 a         1607 c         1844 g         1135 t
ORIGIN      
        1 tgccagccgc ttttgaagaa gcgctgctga acaactgggg ccggcaggtg gacccgcagg
       61 tgctgctgac ggccctgaag ctgcgcaatg tgatgccgga aggcgccgaa ctggcgtatt
      121 cgcagcggca tggattgggc tgggaagaag caaccggcac cttcgtttat attggacatt
      181 cgctggatga gattgatttg aagctggtgg aggcgcaggc cattttgcaa gagctggcca
      241 accagggcca cacccccacc gttgtcagcg tggagaccgt ccacgcgccc tactatcgca
      301 tggagcaata agccatggaa gatttaatcg tcgtcggaat tgatattggc accaccaaaa
      361 cctgtacgct ggtggcgcgg gtggaaggcg agcgcagcct gaggatcctg ggcgtgggga
      421 ttgagccctc gcaggggatc aagaaaggcg tgattgtgga cctgaccgcg gcatcgcagt
      481 ccattgcccg ctcaattgaa aaagcgcagc gctcttcggg gctggagatt acctcagcac
      541 tggtgagcct ggcggggtcg catgtggcct cggtcaacag ccacggcgtg gtgggtattt
      601 cgggccgggc cattgacgag gaagacgtgg cccgcgcgct ggaagcagcc caggcggtag
      661 ccatcccgca caaccgcgag attatccatg tgattcagcg tggatttatg attgacgggc
      721 agggcggcat tcgcgccccg ctggggatgc acggctaccg gctggaagtg gaagcccaca
      781 tcatcaccgc ggcggaatcg accgtggaaa acctgcggca gtgcgtgaat gcagccgggg
      841 ttgaggtctc tcaatttgtg ctgaacccgc tggcgtctgc cgaggtggtg ctttcggaaa
      901 ccgagcggca gatgggcgtg gtggtctgcg atattggcgg cggcacaacc gacctggcta
      961 tttatattga tggggatgtc tggcatacca gtgtgctgcc ggtaggcggc aatcacatca
     1021 ccagcgatat tgcgcacggc ctgcgcctgc caatttcaca ggcggaggat gtcaaaaagc
     1081 agtacggcca tgcccttgaa gccgaagtgg gcgcagatga gactttcacc attcgcccgt
     1141 ttggcgagga aacgccgatc caattcagcc gccgtgagct ggcgacgatt gtgggagcgc
     1201 gcaccgaaga aatcttcgac atggcgctgc aagagatcaa acgctccgga tatgacgggc
     1261 tgctgcccgc cggagtggtt ttgacaggcg gggcaagcca actgcccgga ctgcgggcca
     1321 tggccagcca ggtgctggcg ctgcccgtgc ggctggcccg gccagaaaat ctgctggggc
     1381 tggtggatca actcaattcg ccggcctatt ctaccagcgt ggggctgctt tactgggccc
     1441 tgttgatgag tgaaaccgcc cccgtgccaa cgggccggcg gccacagaaa ggagtgcctc
     1501 ttgactggga taccatcaag aactacctta aacgtctgtt accctaatgc tattttccca
     1561 aattctgtga tttggtagag agaggagaaa aacatggatc tcaccaacca accccctgtt
     1621 gagtcgtttg cccgaatcaa agttattggt gtgggcggcg gcggctgcaa tgccgtcaat
     1681 cggatgattg acgaaggtct gtcaggcatc gagttcgtgg cggtgaacac cgatgcgcag
     1741 gcgttgatgc tggccaaggc tggcacccgg gtgcgcgtgg gcgacaagtc cacccgcggg
     1801 ctgggcgccg gcggcaaccc cgagatgggg cgcaaagcgg cggaggaatc cgctgaagag
     1861 ctgtacgaag tgctgcgcgg cagcgatatg gtcttcgtga cggctggcct gggcggcggc
     1921 accggcaccg gtgcagcccc cattgtggcg cagattgcca aagaggtcgg tgcgctgacg
     1981 attggcgtcg tcacccgccc gttcaccttt gaaggctcgc gccgggcgtc atcggctgag
     2041 gatggcatca accggctgaa ggaacacgcc gacacgttga tcgtgatccc caatgaccgg
     2101 ctgctgcaga ttgtggacaa gcgctccagc ctgcaggacg ccttccgcgt ggccgatgat
     2161 gtgctgcgcc agggcatcca gggcatttcc gagctgatca ccgtccccgg tttgatcaac
     2221 ctggactttg cggatgtgaa ggcgatcatg tcggaaggcg gcgcggcgct gatggcagtt
     2281 ggacacgcca ccggcgagga caaagctcgc gttgcggccg agatggcgat ctccagccaa
     2341 ctgctggaca tcaccattga cggcgcccgc ggcatcctgt tcaatgtgac cggcggcccg
     2401 aacctgacgc tgttcgatgt caatcaggct gctgcaatca tcaaggaaac tgcccacccg
     2461 gatgtgaacc tgatcttcgg tgcggtcatt gaccagaaca tgggcgatga aatccgcatc
     2521 acggtgattg ccacgggttt tgatcgcacg ggtatgcgcc tgcctacgcc acaggaaatg
     2581 cgcgctgccc ggccggcaac cccgcaaccc atctctgcgc aaccggccca aaccaccaac
     2641 ggcgcccgcc agcccgttga ggtgcacagt tcagccccgg ctcccttccc accccaggtg
     2701 gtgaataccg gtgatctgga tgtgccggcc ttcctgcgca aacgcagcga ttcgcagccc
     2761 cgctaggttt tacacctttt gcccggccct gctcccgctt taaggggggc agggctgggc
     2821 ctgcacgcct gcacccagat catgagccga atccgcaccc tgttgtggtg gattctgtgc
     2881 gcggctatca gcacaatgct gctggtggcc tttgtgcgat tctcgggggt caaccataac
     2941 gagaaggctc cgctggcggc gatgctgaac gggcaggcct accgcccata tgtcacccgg
     3001 gcgctggtgc cgggcctggt caatgggctg acggggctgc tgcccccggc cacgcagcag
     3061 gttttgaatg agcggctgaa tggcacttac gccacggcgc ggctgctggc aatgttttcg
     3121 gcgtcgccag agctggcact gcaggggatg ctgacgctgg cggtgatgtg ggcttcactg
     3181 ctggggtttg gggccgcgct gcgagcattg atgagcgcag tgtatgacgc cccgccgctg
     3241 accattgacg tgctcagcct gctggcgctg tgcgggctgc tgccgttctt tgggtttggg
     3301 tatatttacg attttacggt cctgtggctg ttcaccctgg cgctggcctt gatgnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaattt cacctggggg atcatgcctt
     3481 tatgctgcaa aacttcccgg tgctgggcgg gctgaccaca ctcattgggg tgggcctgct
     3541 ggcactgatg gcattgcggc cacggcaaaa gccacccttc ctgctggctg cggcagtgat
     3601 ttttgtgccg ctgctggcgc tatatattcc gtttggtttt ccctatgaaa tccgggtatt
     3661 ctacgagggg tatgccgccg gcacgctgct ggtgatgcaa acgctgctgg gccgccgggc
     3721 gtgggggttg gcgccaaagc ccgagccaaa tcccaacgcc attctttaag tatagacaac
     3781 cgagtcatcc agataccccc agcggtgggg gaaacaccag aaatcagggg gaagatgcca
     3841 gcagtttgcc tgtcctgaga gcggttgcaa tcataaattt tgcccgggaa attttaagat
     3901 tagaacaggc taaatcccct tgccagagtt ggtcatctgt gctagaatga ctgtaccacc
     3961 tgtacggcta tactacatcg atacccccat atatgggggt gtttctctcg aatccaaaag
     4021 tatctcattg taacgccagg aaagacatcc atggtgattg tatatttttc gatatcccca
     4081 aaacaccagg gaggataaaa taatctgaaa tgcgctgtcc ttactgtcag aatgaagagt
     4141 cgcgggtact ggatactacc catgataccc gcggcggcgt ccgccgccgg agagagtgcg
     4201 accagtgcca tcaacgcttc agcacgtatg agcgcccgat tctggcgaca cccctgctgg
     4261 tgaagcgcga tggcacacgc gaagagtttg accgcgaaaa actgatgcgc ggcatccgca
     4321 tttcgtgcgc caagcggccg gtttcggcgg cagaaattga ccgcctggtg ggcgagattg
     4381 aatcggcgct gcagcagatg ggccgcgccg aagtttcggc ccgggtggtg ggagatatgg
     4441 tgattgccgg gctcaagaag ctggatcaga ttgcctacat ccggtatgcc attgtgtacc
     4501 tgcacctgga cgacctgcaa gccattcgca cagaaatcga ccgactgctg gaaggagatc
     4561 actcatgacc gacactgccc ccacccgcgc tgctgattgg accaccaccc cgaccatgcc
     4621 ggaagatctg ccacccgtca atctgacgga gaacgcgcgg caggtgctgg tgcggcgtta
     4681 tgtgcggcgc ggctctaacg gcgaacccgc cgaaaccgtg gaagagatgt tctggcgggt
     4741 tgcttacaac atcgccattg tggagaaggc ctggggcacc gatgtgatgg cgctggcccg
     4801 ggaatattat gaactgctga ccagcaagag cttcttcccc aattcgccca cctttacggg
     4861 cgcgggaaca ccgctgggcc aactggcggc ctgttttgtg ctgccgatca gcgatgacat
     4921 ggggcgggcg tccacgggta tcttccagac gctgcgcgat gcggcgctga ttcagcaaac
     4981 cggtggcggc aacgggtttt cgttctcgcg gctgcgcccc aagggcagcc tggtgatctc
     5041 atctgccgga caggcaaccg gaccggtggg cttcctgcgg gtgtatgacc atgcgtttgg
     5101 cgaagtggcc cagggcggct ctcggcgcgg cgccaatatg gcggtgctgc gcgtggatca
     5161 tccggatatt gaggaattca tcgcctgcaa gacggacgag aatcagatca ccaactttaa
     5221 catttcggtc ggcatcaccg atgcgtttat gcgcgcggtg gaggcggatg aggactggga
     5281 gctgcgcttc ccggacgttc atgatgcccg gtacaagggc tttgatggga ctctggagca
     5341 ggctgaggcg gcaggtatcc cgattctggt gcataaacgg gtacgggcgc gcgaactttt
     5401 tgataagatc gtcaagcagg cgcaccataa cggcgaaccg ggcatgctct tcctggacgc
     5461 ggccaaccgc ggcaacccgg tgccgcacct gtatcaactg gaagcgacca acccgtgcgg
     5521 cgagcagtgg ttgggcggct acgagaactg ctgcctgggt tcggtgaacc tggcacagca
     5581 cctgggcgaa caggggacgg tggattggga aaaattgcgc cgcaatgtgg aactggcaac
     5641 ccgcttcctg gatgatgtgg tggatgccaa tcattatgtg ccggcggtgc cggcgctgaa
     5701 agaagccgct caccgcgccc ggcggattgg gctgggcatt atgggactgg ctgatttgat
     5761 gtaccatgtg ggtgtgcggt acggatcacc tgaggggcag gaatttgc
//
LOCUS       DAOPVS010000114         5786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_13799_length_5786_cov_61.664631, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000114 DAOPVS010000000
VERSION     DAOPVS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_5786_cov_61.664631"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..813
                     /gene="tpiA"
                     /locus_tag="PKL11_08630"
     CDS             58..813
                     /gene="tpiA"
                     /locus_tag="PKL11_08630"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNZ13705.1"
                     /translation="MRTPLVAGNWKMNKTAAEARRLVTEQLPALQAVKNVQRVLCPPF
                     PALMTVAELLAGTDIGLGAQNLHWEASGAFTGEVSPQMVREFCQYVIIGHSERRTYFG
                     ETDQTVNRRIKAALGIGLIPIVCIGETLAENEAGQTADVLKRQIVEGLKDLTIEQGAA
                     LVIAYEPVWAIGTGRAASGEGANAVVADSIRPALKTVFNAQVSQGIQVLYGGSVTGAN
                     AAEFFGQPEIDGALVGGASLKPDFIQIVQAAAQ"
     gene            810..1583
                     /locus_tag="PKL11_08635"
     CDS             810..1583
                     /locus_tag="PKL11_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13706.1"
                     /translation="MNLPPMDGLLWLTGGLLLFIFVQRRLHFELQAIFLLLTRRPTLS
                     EGLFALLFFPGTLLHELSHYLTAKILGVRTGRFSLTPQRLPNGRLQLGFIETAPTDVF
                     RDALIGLAPLITGGLLTAWLGLSRLGLGPVAEALGARNWPLFWQGLADLPALPDFWLW
                     FYLLLAISSTMLPSASDRRAWLPVVSGLALLLVLAILAGAGPWLLSHLSAPLNQGLRA
                     IAVVFGISLVIHLVMLLPAWLIRALISRLTGMEVRPGRV"
     gene            1631..2617
                     /gene="moaA"
                     /locus_tag="PKL11_08640"
     CDS             1631..2617
                     /gene="moaA"
                     /locus_tag="PKL11_08640"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNZ13707.1"
                     /translation="MLLDRYGRQITYLRVSVTDRCNMRCVYCMPPEGVQLQSHAAVMR
                     YEEIAEVVRVAASQGIREVRLTGGEPLVRLDLAELVRLIAAIPGIEDISLTTNGLLLG
                     KYARSLADAGLRRVNVSLDTLRPDRFTRITRGADLSQVWQGLTAAEAAGLKPIKLNMV
                     VMRGVNSDELIDLAQLTLEHDWHVRFIELMPVQNQSAWGQDFPSPQDAYFPIAEMLAQ
                     LAPLQLEPIFTDIGRGPADEYRIPGAPGRLGFISPLSEPFCHECNRLRLTADGNLRPC
                     LLSDIEIPVLSAIRRGESPLPLILQAVEQKPESHELSQNHTPSGRCMRQIGG"
     gene            complement(2671..3456)
                     /locus_tag="PKL11_08645"
     CDS             complement(2671..3456)
                     /locus_tag="PKL11_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNZ13708.1"
                     /translation="MKLFQVDAFTSAPFAGNPAAVCILSEKQDEQWMQQVAREMNLSE
                     TAFLLKQPDGFSLRWFTPATEVNLCGHATLASAHVLWEVGLISVTEQAQFHTRSGLLT
                     AKRHGNLVELDFPALSEEPAEAPAELIAALQVTPRYVGKFGAKYLIEVNDEETVRKLD
                     PDFPALRKMPGRGIVVTSAASTAPYDFVSRYFAPWVGVDEDPVTGSAHCCLGPYWGQR
                     LGKQELKAYQASARGGEIHIRLGQERVYLSGNAVTVFSGTLHA"
     gene            3622..4056
                     /locus_tag="PKL11_08650"
     CDS             3622..4056
                     /locus_tag="PKL11_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNZ13709.1"
                     /translation="MPDPRLLTLTEKLKRHGYRLTPQRLAILKILADSEGHPTVEQIH
                     ARVHVEFPTTSLATVYKTIAMLKAEGEVLELGFGNISRYDGSKPQPHPHLICTRCQSI
                     VDPDEHLFADLSKDLTKKYGYQITSYRIDYFGICPECQHQNV"
     gene            4140..4634
                     /locus_tag="PKL11_08655"
     CDS             4140..4634
                     /locus_tag="PKL11_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNZ13710.1"
                     /translation="MLSKPVYEKMNAQITHEMASAYLYLSMAAYFENENLPGFANWMR
                     VQFSEEQEHALKFFEYILDRGDKVALQAIPQPAIDFKSPEDVFKQTLAHEQKVTSLIL
                     AIYAEAVAQNDVAAQIFLQWFINEQVEEEKNASQILDVLGKIGSSAGGLYQLDHQLGK
                     RKGD"
     gene            complement(4694..5320)
                     /gene="upp"
                     /locus_tag="PKL11_08660"
     CDS             complement(4694..5320)
                     /gene="upp"
                     /locus_tag="PKL11_08660"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNZ13711.1"
                     /translation="MPNLFVSTHPLVAHKLTRLRDVRTEPKKFRELVREIANLLAYEA
                     TADLAISPMPVQTPLALANGYELQQKIGLVPILRAGLGMVEGVWDMMPSAEVWHIGLY
                     RDERTLKPVEYYNKLPTEPTVSVCLILDPMLATGGSAVATVEVLKRWGAKKIKFVGLI
                     GAPEGVARLQQAHPDVPIHLAAVDERLNEHGYILPGLGDAGDRQFGTA"
     gene            5395..>5786
                     /locus_tag="PKL11_08665"
     CDS             5395..>5786
                     /locus_tag="PKL11_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ13712.1"
                     /translation="MTDDTQRLVDPEPRPDDRIDQALRPRRLNDLIGQSQVKENLAIL
                     IAAARHRAEPLDHVLFYGPPGLGKTTLAHVLANEMGVNIKITSGPAVERAGDLAAILT
                     NLRAGDILFIDEIHRLGRAIEEVLYPAM"
BASE COUNT         1223 a         1760 c         1420 g         1383 t
ORIGIN      
        1 aaaatcattc aggataccgg acttcctgag atcaataaac cgtaaaggaa catcattatg
       61 agaacaccac ttgttgctgg taactggaaa atgaacaaaa ccgctgccga agcgcgccgc
      121 ctggtcacag agcagctccc cgctctgcag gctgtcaaaa atgtgcagcg cgtcctgtgc
      181 ccgcccttcc ccgcgctcat gacggttgcc gagctgcttg ccggcaccga tatcggcctg
      241 ggcgctcaga atcttcactg ggaagcctct ggcgcattca ccggcgaagt ttctccccag
      301 atggtgcgag agttctgcca gtacgtcatc attggccatt ctgaacgccg cacctatttc
      361 ggcgaaacgg accagaccgt taaccgccgc ataaaagcag ccctgggcat tggcctgatt
      421 ccgattgtgt gcattggtga aaccttggcc gaaaatgaag ccggtcagac tgccgatgtg
      481 ctcaagcgcc agattgtgga aggcctgaaa gacctgacca tcgaacaggg tgcggctctg
      541 gtcatcgctt acgaaccggt ctgggccatc ggcaccggcc gcgctgccag tggtgaaggc
      601 gccaatgccg tggtcgcgga tagcattcgc ccggccctga aaaccgtttt caatgcccag
      661 gtctcgcagg gcattcaggt gctctatggc ggctctgtaa ccggcgccaa cgcggccgaa
      721 ttttttggcc agcccgaaat tgatggcgcc ctggtgggcg gcgccagcct aaaaccggat
      781 ttcatccaga tcgtccaggc tgccgctcag tgaatttgcc cccaatggac ggcctgctct
      841 ggctgaccgg cgggttgttg ctctttatct ttgtgcagcg ccgcttgcat tttgaactgc
      901 aggccatctt ccttttgctc actcgccgtc cgaccctctc cgagggcttg tttgcgttgc
      961 tgttcttccc cggcacgctg ctccatgagc tgagccatta tctcaccgcc aaaattctag
     1021 gcgtgcgcac cgggcgcttt tcgcttaccc cccagcgcct cccgaatggc cggctgcagt
     1081 taggcttcat tgagaccgcc cccaccgacg tctttcgcga tgcgttgatc ggtctggccc
     1141 cgttaatcac cggtggattg ctcactgcct ggctggggct ttctcgcctg ggtctcggcc
     1201 cggttgccga ggccctgggc gcccgcaact ggccgctctt ctggcagggg ctggctgatt
     1261 tgcccgccct cccggatttt tggctttggt tctatttgct gctggccatc agcagcacca
     1321 tgctcccctc agcctcagat cgtcgcgcct ggctgccggt ggtatcgggc cttgcactgc
     1381 tgctggtcct ggcaatcctg gcgggcgccg ggccctggtt attgtctcat ctatctgcgc
     1441 cgctcaatca gggcttgcgc gccattgcgg ttgtatttgg catcagtctg gtcatccatc
     1501 ttgtgatgtt gctgcctgcc tggttgatcc gtgcattaat cagccggctt accggaatgg
     1561 aagtccggcc tgggcgggtc taaattgaca ttctttatcc aaatgtgtac aatgacctga
     1621 gaggtgcccc atgctcctgg atcgttatgg ccgacaaatt acctacctcc gggtctctgt
     1681 cacagaccgc tgcaacatgc gctgtgttta ctgcatgccg cctgagggcg ttcagcttca
     1741 atcccatgcc gctgtcatgc gctatgaaga gatcgccgag gtagtccgtg ttgctgcgtc
     1801 ccagggcatt cgagaagtcc gtctgaccgg cggcgaaccc ctggttcgtc tggaccttgc
     1861 tgaactcgtt cgcctgattg cagccatccc tggcattgaa gatatcagcc tcaccaccaa
     1921 tggactattg ctgggcaaat atgcccgctc actggcggat gccgggctgc ggcgcgtaaa
     1981 tgtcagcctg gacaccttgc gccccgaccg gtttacccgc atcacccgcg gcgccgacct
     2041 gtctcaagtc tggcagggtc tgaccgccgc tgaagcggct gggttgaaac ccatcaaatt
     2101 gaatatggtg gtcatgcggg gtgtcaattc tgatgagttg attgatctgg cccaattgac
     2161 ccttgagcac gactggcatg tccggttcat tgaactgatg ccggttcaaa atcaatcggc
     2221 ctggggccag gatttcccct cgccgcagga tgcctacttc cccatcgctg aaatgctggc
     2281 ccagcttgca cccttacagc ttgagcccat ttttactgat attggccgcg gccctgcaga
     2341 cgaatatcgc attcctggtg ctcccggtcg cctgggcttt atctcacctc tcagtgagcc
     2401 cttctgccat gagtgcaatc gcctgcgtct gacggctgac ggcaacttgc gcccctgcct
     2461 gctctcagac attgaaatcc ccgtcctctc agccattcgc cgcggtgaat cccccctccc
     2521 gttgatcctg caggcggtgg agcaaaaacc cgaatcccac gaactgagcc agaatcatac
     2581 cccctctggt cgctgcatgc gtcagattgg cggttgatgg cccaataatg cagtcatttc
     2641 tgaggctgtc ttacatcctg gtattctcag ttaagcatgt agtgtcccgc taaaaacggt
     2701 cactgcattt ccactcaggt acacccgttc ttgccccagg cgaatgtgaa tctctccacc
     2761 gcgcgcagaa gcctgataag ccttcagttc ttgtttccct aaccgctgcc cccaataggg
     2821 ccccaggcag cagtgcgccg agccggtcac cgggtcttca tccacgccga cccagggcgc
     2881 aaaatagcga gaaacaaaat cgtatggggc tgtcgaggca gcgcttgtca ccacaatccc
     2941 gcgtcctggc atctttctca atgccggaaa atctgggtca agcttgcgca cggtttcctc
     3001 atcattcact tcaatcaagt attttgcccc aaacttgcca acataccggg gcgtcacctg
     3061 cagcgctgca atcagctccg ccggcgcctc agccggttcc tcgctcagag ccggaaaatc
     3121 cagctcaacc aagttgccat gccgtttggc cgtcagcagc ccgcttcggg tgtgaaattg
     3181 agcctgctcg gtaacgctta tcagccccac ttcccataaa acatgggcgc ttgccagggt
     3241 tgcatgtcca cacaaattga cctctgtggc tggcgtaaac caccgcagcg agaacccatc
     3301 cggctgtttc agtaaaaatg ccgtctcaga tagattcatc tcacgcgcaa cttgctgcat
     3361 ccattgttca tcttgctttt cggacagaat gcacaccgcc gccggattcc ccgcaaatgg
     3421 ggcactggta aacgcatcaa cttgaaaaag tttcatatcc attggattaa caccaccata
     3481 gccgcacgag ttaattgatt ttactccggt tggcgcaacc tgaccaattg gattctttga
     3541 aaccccttga cttttaatta ttaattgcta taatatagat aataattatc ttataataat
     3601 aattcttatt tagggatacc tgtgcctgat ccacgcttat taaccctgac cgaaaaactg
     3661 aaacgccacg gctaccgcct gacgcctcag cgtttagcca ttcttaagat cctggcagac
     3721 tcagaagggc accccacggt tgaacaaatc catgcccgcg ttcatgtcga atttcccacc
     3781 accagccttg ccacggtcta caaaacaatt gccatgttga aagctgaagg cgaggtattg
     3841 gagcttggct ttggaaatat cagccgctat gatggctcta agccacagcc ccacccacat
     3901 ctcatttgta ctcgatgcca gtcaattgtt gaccccgatg agcatttgtt tgctgattta
     3961 tccaaagact tgaccaaaaa atatggttac cagatcacaa gctatcggat tgattacttt
     4021 ggcatttgcc ccgaatgtca gcaccaaaat gtctgatttt cgaatcctaa tgtatacttg
     4081 aggtaagatt acctcttgga taaacctata actctcacat caccttgaaa ggaaaaacta
     4141 tgttaagcaa acccgtgtac gaaaaaatga atgcccagat tacccatgag atggcctctg
     4201 cctatctcta cctctccatg gccgcctatt ttgaaaacga gaaccttcct ggctttgcca
     4261 actggatgcg cgttcagttt tcagaagagc aggaacacgc cctgaagttc tttgaatata
     4321 tccttgaccg cggcgataag gtcgcgctcc aggctattcc tcaacctgcg atcgatttca
     4381 aatccccgga agacgtcttc aaacaaactc tggcgcacga acagaaagtc accagcctca
     4441 ttcttgcgat ctatgctgag gccgtggctc aaaatgatgt cgccgctcag attttcctgc
     4501 agtggttcat caacgagcag gtcgaagaag aaaagaacgc ttcccagatt ctggatgttc
     4561 tgggcaagat tggctccagc gccggcggtc tctaccagct tgatcatcaa ctgggcaaac
     4621 gcaagggcga ctaaccgccg ctattcagcc tctaaatcag caccgggcga gattttctcg
     4681 cccggtctgt tttttatgcg gttccaaact gccggtcgcc ggcatctccc agccctggca
     4741 gaatatatcc gtgttcattc aaccgctcat caaccgccgc cagatgaatc ggtacatccg
     4801 ggtgtgcctg ttgcagccga gctacgccct ccggtgctcc aattaacccc acaaatttga
     4861 ttttcttggc cccccaccgt ttaagcacct ccaccgttgc cactgccgaa ccgccggtcg
     4921 ccagcatggg gtccagaatc aggcacaccg aaacggtcgg ctcggtgggc agcttgttat
     4981 aatactccac cggtttcaat gtgcgctcgt cgcgatacag cccgatatgc cacacctctg
     5041 ccgagggcat catatcccac acgccttcaa ccatgcccag cccggctcgt aaaatcggca
     5101 ccagcccgat cttctgctgc aattcatatc cgtttgccag cgccaggggt gtttgcaccg
     5161 gcataggtga gatcgccagg tcagccgtgg cttcataggc cagcaaattt gcaatctcgc
     5221 gcaccagttc acggaatttc ttcggctcgg tccgcacatc acgcaggcga gtcagcttgt
     5281 gcgctaccag cgggtgagtg gatacaaaca gattgggcat cttctactcc tgatatcaca
     5341 ttgggatcat ttacattata tcccacagac ccaagaggcg gtataattca ccctatgaca
     5401 gatgacaccc aacgccttgt tgatccggag cctcgcccgg atgatcgcat tgatcaggcc
     5461 ttgcgcccgc gccgtctgaa cgacctgatt gggcaatcac aggtcaaaga aaacctggcg
     5521 attttaatcg ctgcagcccg ccaccgtgct gaacctttgg atcatgtcct cttttatggt
     5581 cctcctggcc ttggcaaaac caccctggcc cacgtgctgg caaatgaaat gggtgtcaat
     5641 attaaaatta cctccggccc ggccgttgaa cgcgctggtg atctggctgc cattctgacc
     5701 aacctgcgcg ccggcgatat cctctttata gatgaaattc accgcctggg acgggcaatt
     5761 gaagaagttc tgtaccccgc catgga
//
LOCUS       DAOPVS010000115         5732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_13973_length_5732_cov_42.209970, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000115 DAOPVS010000000
VERSION     DAOPVS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13973_length_5732_cov_42.209970"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..997)
                     /locus_tag="PKL11_08670"
     CDS             complement(269..997)
                     /locus_tag="PKL11_08670"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ13713.1"
                     /translation="MKVIGLHSFRRGVGKSTLAANLAYLLAAAGKRTILMDVNLYSPA
                     LEIFFKLKRDLSAPTLNDYLSGASPITELVCQPIPDLPLHLVPASSRTADMISALRPP
                     WDTTALLNGVAELDASLNPDYLLLDMASGLHEESILCMSVCNLILEVLRADAQNYQGT
                     AIMVDVARRLQVEPRLVLNDAPPDLDLPVVQQRLEKAYDCPVAAMFTHSDQMMALGAV
                     GGLFVRQNPDAEISWRLKELAASL"
     gene            complement(1002..2063)
                     /locus_tag="PKL11_08675"
     CDS             complement(1002..2063)
                     /locus_tag="PKL11_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNZ13714.1"
                     /translation="MVEVPTQPQPKRKGFYMSLRLKLLISLTLLFTAAFVGIFYWFYN
                     FVSDQTWNLIKSDAQSTLNGALEGIDGDQFEALVQEAQPRADGYTDDPRYWEHVQWLY
                     HVHEVEPRAFVYTYVKGTKENEVIFIGSHGAAMTPPEGAPFLAPYEEPADNPLLTRGL
                     SAPVVNEFFLESSGADDFGAWGVTTCTPIKNSKGETVGALGIDFQTDYVKKVQATLRT
                     LLLVVFGIIYIGFFLIIWFYSNSLARPITRLTHIARQIGDGHYDQDLAGLTKARLRDE
                     IGTLAEVFDIMVGKVEHREQSLRQEVQRLQIEIDESKRQNQVNEIVETEFFQELVTRA
                     QEMRDRKRARLAGELPPQE"
     gene            complement(2075..3565)
                     /locus_tag="PKL11_08680"
     CDS             complement(2075..3565)
                     /locus_tag="PKL11_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13715.1"
                     /translation="MLLAEYPETGLQIHELAHQGGGLLPACLEVFAEQFPEYAAEPVN
                     LDELRIAAESAPDPAALFQPHLWLITVHGQPAGMTFFLYNRRRAIGLLLFIAIRAEHR
                     KAAPAGVARLSDWVLEHMLHQIRSDGEAAGKPALGLAAEVDYPHLMARYEQMGMLPVP
                     AAYAEPLAGMTASMVGTEALEQIVFHPLLLGMFPLGALNVLSPENMGEIVLTFLQDYY
                     ELPLDHWAVQRALDSLGLRLPRPASSCLYSEKGELMVAELAHDVSVTVVDLLRTPSAA
                     DVDALMALFDELFPQYAPIPYVVNELRQMTWEPAEIRSPFIRHLWLVRVNDQPAGMTF
                     FCYHKERNLAIGVYLAVRPEFRRLVINGYHRLAEYLITQTHQVIATDAQACGHPIPSG
                     YIAEVDVSRLMQQYRQYGFILLPVSYHEPRLPDGCTVLLPPDSLDYHPINLGFFPRNP
                     QTFDELPSRDLIRSGLLTFLVDFYGIPLDHWSVQQALQSIPTDLPE"
     gene            complement(3569..4906)
                     /locus_tag="PKL11_08685"
     CDS             complement(3569..4906)
                     /locus_tag="PKL11_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13716.1"
                     /translation="MQTPNRIVRLHPGEFPLVLILGLVLLVNSLTMQLGGIVAVSGFL
                     NTGGINSILIVYLVDYALIFLSGVFQSLIVDRFDRTKLVKAMALLFALVYLVLRLLFW
                     LGAPDWLNYAITFVFAEQQFVFFPLVFWVLANDLYSMEQSKRLFPLISSLSFVGELIG
                     IGLAIAAPAIMLALNVSIEEVLTLNAMLYLGAWLSLHFGLRGLKPRTTVRQKQTLRET
                     LVEGWDFVRNVDSFRYLMYVIIALAACWLMLEFRFLGITDQTYPDQFSYQRFFSIFRL
                     VLALGGFAMQALFTSRLLAAMDLRRAFFILPAAALIGAVLMLIFPGATPVIAGIGIYM
                     LARLTVHDSAIKSLESLIPEERRGRASTFLDGYLPAVGVIGGVLLTGAVVLIGQWTQT
                     DLHWLYLVLAAVAAALSIFWVFKMQKVYDNSLLNWRLKRRRTTATSVMDKLDF"
     gene            complement(4919..5677)
                     /locus_tag="PKL11_08690"
     CDS             complement(4919..5677)
                     /locus_tag="PKL11_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNZ13717.1"
                     /translation="MSKIVSIHSFRGGTGKSNSTANLAAQIALTGKRVGIVDTDIQSP
                     GIHVLFNVQEEATVHSLNDYLWGKCEIQDAALDVTPNLGVPISGKIYLIPSSIKTGEI
                     VRVLREGYDVSLLNDGFQKLVEELNLDVLLIDTHPGLNEETLLSIAISDVLIIIMRPD
                     QQDYQGTAVTLDIARKLGVARIALLVNKIPQVFDLADVRARVERIYQIEVAGLLPHSD
                     DMMALASAGVFSIKFPDHPISKIYRQVVTQQILG"
BASE COUNT         1375 a         1495 c         1720 g         1142 t
ORIGIN      
        1 acaatgctga tgagcaagcc aacccaaaat tgccagcgtt tgagcatgga gtattagcct
       61 cagggtaata gagtgttcct attgtaacac agacagattg gacccatttg attgtaagac
      121 aggggggatt aaatgggcaa gcgcctgagt gaggggggca cccagacgct tgcttatttg
      181 tattctagca tatcaccgga ttttatacaa gatggttttt ggctatattt aaagatttta
      241 caaaaagcac aggaaacgac cagccgcgct aaaggctggc agccaattcc ttaagccgcc
      301 agcttatttc ggcatcggga ttctggcgga caaacaaacc gccaacggcg cccagcgcca
      361 tcatctggtc ggagtgggtg aacatggccg cgaccggaca atcgtaggct ttttcgagcc
      421 gctgctgaac aaccggcagg tccaggtcgg ggggggcgtc attgagcacc aggcggggct
      481 ccacctgcag gcgccgggcg acatccacca tgatggctgt gccctggtag ttctgggcgt
      541 cggcgcgcag cacttcgagg atcaggttac agacggacat gcacagaatc gattcttcgt
      601 gcaggcccga ggccatatcc agcagcaaat aatccgggtt caggctggca tcaagctcgg
      661 ctacgccgtt cagcagcgcg gtggtatccc acggcgggcg gagggcagag atcatatcag
      721 cagtgcggct gctggccgga acaaggtgca gcggcaggtc gggaatgggc tgacaaacca
      781 gctcggtgat ggggctggcg ccgctcaggt agtcgttgag ggtgggggca gacaggtcgc
      841 gctttaactt gaagaagatc tccagcgcgg gcgaatagag gttcacatcc atcaggatgg
      901 tgcgctttcc ggcagcagcc agcaggtaag ccaggttggc tgccagggtg gatttgccca
      961 ccccgcggcg gaatgaatga aggccgatga ctttcatccg gttactcctg aggcggcagt
     1021 tcacccgcca ggcgggcacg tttgcgatca cgcatctcct gggcgcgggt taccagttcc
     1081 tggaagaatt cggtttcgac aatttcgttc acctgattct ggcgtttgga ttcgtcaatt
     1141 tcaatttgca ggcgctgaac ttcctggcgc agcgattgtt cacgatgctc gaccttgcca
     1201 accatgatat cgaacacttc ggccaaagtg ccgatttcat cgcgcaggcg agccttggtc
     1261 aggccagcca agtcctggtc atagtgacca tccccaatct gacgggcaat gtgcgtgagg
     1321 cgggtgatgg ggcgcgccag gctgttggag tagaaccaga tgattaagaa aaagccgata
     1381 tagatgatcc caaaaaccac cagcagcagg gtgcgcaagg tggcctgcac ctttttgacg
     1441 tagtcggttt gaaaatcaat ccccaatgcg ccgactgttt cgcctttgga atttttgatc
     1501 ggcgtacagg tggtaacacc ccaggcgcca aaatcatcag ccccggagga ttccagaaag
     1561 aattcattga ctaccggggc agacaggcca cgggtcagca aggggttgtc ggctggctct
     1621 tcgtagggtg ccaggaatgg ggcgccttca ggaggtgtca tggctgcgcc atggctgcca
     1681 ataaaaatga cttcgttctc tttggtgccc ttgacgtagg tataaacaaa ggcgcgcggt
     1741 tccacttcgt ggacatgata gagccactgc acatgctccc agtagcgcgg atcgtcggtg
     1801 taaccatcgg cacgcggctg ggcttcctgg accagggcct cgaactgatc gccatcaatg
     1861 ccttcaaggg cgccattcaa ggtactctgc gcatccgatt taatcaggtt ccaggtctgg
     1921 tctgagacaa agttatagaa ccagtagaaa attccgacaa aggccgccgt gaacagcagc
     1981 gtcaggctga tgagtagctt gagccgcagg ctcatataaa agcctttgcg tttgggttgg
     2041 ggttgggtgg ggacttcgac catggtgaga gctcctattc tggtagatcc gtcgggatag
     2101 actgcaaagc ctgctgcaca gaccaatgat ccagggggat accgtaaaag tccaccagga
     2161 aggtcagcag gcccgagcgg atcaagtcgc gcgagggtag ttcgtcaaat gtctggggat
     2221 tgcgtgggaa aaagcccagg ttgatggggt gataatccag cgagtcgggc ggcagcagaa
     2281 ccgtgcagcc atcgggcagg cgcggctcat ggtaactgac cggcaggagg atgaaaccat
     2341 actggcggta ctgctgcatc aggcggctga catccacttc ggcaatgtac cccgagggga
     2401 tgggatgacc gcaagcctga gcatctgtgg caatgacctg atgggtctga gtaatgagat
     2461 attccgccag gcggtggtag ccattgatca ccaggcggcg gaactccggg cgcactgcca
     2521 ggtacacgcc aatcgccagg ttgcgttctt tgtgatagca gaagaaggtc atgccggcgg
     2581 gttggtcatt aaccctgacc aaccacagat gccggataaa aggcgacctg atctcggcag
     2641 gctcccaggt catttgtctt agttcattga ccacgtaggg aatgggcgcg tactgcggga
     2701 aaagctcatc aaagagcgcc atcaatgcgt ccacatctgc ggcagatgga gtacgcagta
     2761 aatccaccac ggtcaccgat acatcatgag caagctcagc gaccattaat tcccccttct
     2821 ctgaatagag acagcttgat gccgggcgcg gcaggcgcaa gcccagcgaa tcgagggcgc
     2881 gctgcacggc ccaatgatcc aatggtagtt catagtaatc ctgcaaaaag gtcagtacaa
     2941 tttcgcccat gttctccggc gatagcacat tcagcgcccc gagcggaaac atgcccagca
     3001 agagcgggtg aaaaacgatc tgttccagcg cttcggtgcc gaccatactg gcggtcatgc
     3061 cagccagcgg ttcggcatag gctgccggga caggcaacat gcccatctgt tcgtagcggg
     3121 ccatcaggtg agggtaatcc acctcggcag caaggcccag ggcgggtttg ccagcggctt
     3181 cgccatcgga gcggatctgg tgaagcatat gctccagaac ccaatcggag agccgggcaa
     3241 cgccagccgg ggcggctttg cgatgttcgg cgcggatggc gatgaagagc aacaaaccga
     3301 tagcgcggcg gcggttgtat agaaagaatg tcatgccggc gggctggcca tggaccgtga
     3361 tgagccagag gtgcggctgg aacagggcgg cagggtcggg cgccgactct gcggctatgc
     3421 gcagctcgtc caggttgact ggctcggcag catattcggg gaattgttcg gcgaacacct
     3481 ccaggcaggc tggcagcaag ccaccaccct ggtgcgccag ttcatgtatt tgcaatccgg
     3541 tttcggggta ttctgccaga agcatgggtt agaaatccag tttgtccatc acacttgttg
     3601 ctgtggtacg gcggcgctta agccgccagt tgagcaggct gttgtcgtac accttctgca
     3661 tcttgaagac ccagaagatg gacaaagctg ccgcgaccgc agccagcacc agatagagcc
     3721 agtgcaggtc tgtctgcgtc cattgaccaa tcaacaccac tgctccggtc agcagcacac
     3781 cgccaatcac acccactgcc ggtaaataac catctaaaaa tgtgctggca cggccacggc
     3841 gttcttcagg gatcagcgac tcgagcgatt tgatggctga atcgtggacg gtgaggcggg
     3901 ccagcatgta gatgccaatg ccagcaatga ccggtgtagc gccagggaag atcagcatca
     3961 acacagcgcc aatcaatgcc gccgccggca gaatgaagaa ggcgcggcgt aaatccatag
     4021 ctgccagaag ccggctggtg aagagggcct gcatggcaaa gccacccagg gctaacacga
     4081 gccggaagat actgaagaaa cgctgataac tgaactggtc cgggtaggtt tgatcggtaa
     4141 ttccgaggaa acggaattcc agcatcagcc agcaggctgc cagggcgatg atgacataca
     4201 tcagatagcg gaaggagtcg acattgcgga caaagtccca gccttccacc agggtttcgc
     4261 gcagggtttg cttttgccgg acggtggtgc ggggcttgag gccccgcaag ccgaagtgca
     4321 gcgataacca ggcacccaga taaagcatgg cattgagggt taatacttct tcgatggaga
     4381 cgttcagcgc cagcatgatc gccggggcag caatggccag gccaatgcca atcaattcgc
     4441 ccacaaagct gaggctggag ataagtggga acaggcgttt ggactgctcc atgctgtaca
     4501 ggtcatttgc cagcacccag aaaaccagcg ggaaaaagac aaattgctgc tcagcaaaca
     4561 caaaggtgat ggcatagttc aaccagtcgg gagcgcccag ccagaagagc agccgcaaga
     4621 ccagatagac cagcgcaaag agcagcgcca tggctttgac cagtttggta cggtcaaacc
     4681 gatctacaat gagggattga aaaacgccag acaggaagat gagcgcataa tccaccagat
     4741 agacaatcaa aatggaattg atgccgccgg tgttcaagaa accactgacc gccacaatgc
     4801 ccccaagctg catggtcaac gaattgacca gcagcaccag ccccaggatc agcaccaggg
     4861 gaaactcgcc tggatggaga cgaacgatgc ggttaggggt ctgcatagat gctcctgact
     4921 aacctaagat ttgttgggtc acaacttgtc gataaatctt gctaatggga tggtcgggga
     4981 atttgatgct gaagacgccc gcagaggcca aagccatcat atcatccgaa tggggcagca
     5041 aaccagccac ttcaatctgg taaatgcgtt caacccgggc ccggacatct gccaggtcga
     5101 aaacctgagg gatcttattg accaacaggg cgatgcgcgc cacgcccagc ttgcgggcaa
     5161 tatccagcgt tacggcagtg ccctgataat cctgttgatc ggggcgcatg atgatgatca
     5221 ggacatcgga gatggcaatg gagagcaggg tttcttcgtt gaggcccgga tgggtgtcga
     5281 tcaacagcac atccagattc aattcctcta caagcttctg aaagccatca ttgagcaggc
     5341 tgacgtcgta gccttcgcgc agcacccgga cgatctcgcc cgttttgatg ctggatggga
     5401 tgagataaat ttttccagaa atagggacgc ccaggttggg ggttacatcc agggcggcgt
     5461 cctgaatttc gcacttgccc cagagatagt cattcaggga atgcaccgtg gcttcttcct
     5521 gcacattgaa gagtacatga atgcccggcg actggatatc ggtatcgaca atgccgacac
     5581 gtttacccgt cagggcaatc tgtgctgcca ggttggcggt ggagttggat ttgcctgtgc
     5641 cgccgcggaa tgaatgtatg gatacgattt tgctcacaag attttcctcc aatctttatg
     5701 attctttggt ttcgggttcg gtcggggttt gt
//
LOCUS       DAOPVS010000116         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14064_length_5705_cov_44.560177, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000116 DAOPVS010000000
VERSION     DAOPVS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14064_length_5705_cov_44.560177"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2688
                     /locus_tag="PKL11_08695"
     CDS             157..2688
                     /locus_tag="PKL11_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13718.1"
                     /translation="MKIIALLAVLTVLIQPMNVAALAPQAELRVCSSGCPYTILQDAI
                     DAAEPGDSIQIAAGTYSDLNTLGGLSQIAYITKSLTLRGGYSPDFSTWDPATYITTLD
                     AATAGRVVYASGHAELTLAGLHMTHGNATGLGGDSLYDEDAGGAIYINGGVVTMTDCT
                     VENSIASQSSQPGNYSANGGGVYIASSTSVTLTHNLIQNNIAATVAGDYAGLGGGILI
                     FYSNSLLEDNTITGNIATTLSAGQGGGLNVFGGTAVLNNNTITFNTASQSQADSYSAG
                     EGGGIFAGYPVTMTGNLIADNTAGYSHGNGGGLYLMGAAILTNNQIVRNTGADPASEH
                     QTSYGGGLYLFDGDSALITGNLFENNLAGERGGAIYLDEGSPTFSENIIRGNHASIKG
                     YGLGGGLYLDEAPAHLSNNQIIENIAMDENVGSAGLSGFGGGLYLDNSSAILTGNLIS
                     GNRGSVATIYGEGRGGGVYILGGSPQLTTNQITDNLAANAYYDTGFGGGVYLESSGAT
                     LTRNLIDGNTASSIDNGYGGGIYTRGWHGAAPVLDGNVITNNTASFHSLVNDGFGGGV
                     AVYRFGETVPVILRNNVIANNDADDFGSGLWVGGNYETQPVGVLLEHNTFANNQNGDA
                     AISLQYALTLQMTNNIISGHNLGVFAMEDGTVTMDGTLWHQNTADTGGAGTITHTNDL
                     TGDPLYADAANFNYHLTSGSAARDAALASSIVSDLDGQARPNPDTNLPDLGADEFYVT
                     PPSAVTISGPGTAYLGLPATFTAQTSPADATLPLTYTWQATGQDPVTHPSGLVDTVQF
                     TWTEPGEQQITVIVTSAYGGPVTTTLIVLVQVAVIKKVYLPLVVR"
     gene            3084..3641
                     /locus_tag="PKL11_08700"
     CDS             3084..3641
                     /locus_tag="PKL11_08700"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ13719.1"
                     /translation="MDLRSTILGLLNWTPLSGYDLKRIISDSDIFYWSGNNNQIYKTL
                     LELEKEDLVSHQVQLQESLPAKKIYSITNKGMVALYQSLLAVPEPPELRKSFLVQLAL
                     AESLSDKEIRTLLEKYEEEIAYRLRMYQGQAVRLNNSPARSQREKYLWKRIAENLIET
                     YQTELNWVLQTKQEFSEQKFLDLEP"
     gene            3694..4539
                     /locus_tag="PKL11_08705"
     CDS             3694..4539
                     /locus_tag="PKL11_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ13720.1"
                     /translation="MNTISVYKSSAGKAKIMALYDSVLERWPVTHETFMLPTRQGDTF
                     VIASGEKSAPALMLLHGSASNAVSWIGDIVEYSRHFRVYAVDLPGEPGKSTENRPAWV
                     GSDYAVWLEDVLDGLNITKTSLLGISQGGWTALKFATIYPQRVNKLVLLASGGILPAR
                     SSFILKAVIYSMFGHWGSQKIIQMVNGKQPMHPEAIEFMEAIYTHFNTRVEKEYLYSD
                     DELIRLNMPVMYIGGEQDALFDTAAAAKRLQKLVPLLKVVLIPNMGHALINLSAETLP
                     FLTAS"
     gene            complement(4666..>5705)
                     /locus_tag="PKL11_08710"
     CDS             complement(4666..>5705)
                     /locus_tag="PKL11_08710"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ13721.1"
                     /translation="GILLNRINRGSDEALGCYLEALNAAHEMGDRHQESLVLVNLGIA
                     YRSRRAYDQAIQIYERALRIQEEIDDPLHQSATLINLSILMNYQDRRKDALRLLEQAI
                     EIKQRIGDQRGESIALSGIGIIWNYLGQYQRAEENFLKVLQIRRAIGERRGEINALSN
                     LTTSALGQGELGLALDYAQRAVAVAEEIQIPQSKAYGYQSLGDVLYAHLRYQEAVQYY
                     QQAVELFETTNDLEECLSTRSKIALAFLALGQLDAAQTEVEVVFRQIEKGLETPEDSC
                     PIPLVVYQAGYRVLAASQSAKAQQVLETGYQALMQKADQIDDSALRQSFLGNVAENRE
                     LLAAYEQAQQL"
BASE COUNT         1357 a         1560 c         1395 g         1393 t
ORIGIN      
        1 tgtggatacc gatcaaccgg atgccgtgcg cggcgccctg caggggcttg gcagctcgga
       61 gccgccattg cccttcgaca acccggcgca cctgctgtat cagatcaact acctcacaac
      121 cgaacaatta aaatcgcttt ccagggagaa tgactcatga aaatcattgc actgttagcc
      181 gttctgacag tcttaatcca gcccatgaac gttgccgctc ttgcccccca ggcagagctg
      241 cgcgtctgta gcagcggctg cccctacacc atccttcagg atgccattga tgctgcagaa
      301 cccggcgaca gtatccagat tgcagccggt acctattcag atctgaacac gctgggcggt
      361 ttgagccaga ttgcctatat caccaagtca ctgaccctgc gcggtggtta cagtccggat
      421 ttcagtacct gggaccctgc cacatacatc accactctcg atgccgccac agccgggcgt
      481 gtggtttatg ccagcggaca tgcagagctc accctcgcgg gtctgcatat gacccatgga
      541 aatgccactg gcctgggtgg cgacagcctt tatgatgaag atgccggtgg agccatctat
      601 atcaacggcg gcgttgtcac catgaccgac tgcacggtgg aaaatagtat tgccagtcaa
      661 agttcgcagc ctggcaatta ttcggcgaat gggggtgggg tgtatatcgc ctccagcaca
      721 tccgtgacgc tgacccacaa cctgattcag aataatattg ccgccacagt cgccggggat
      781 tacgcggggc ttggcggtgg cattttgatc ttctattcca acagcttgct ggaggacaac
      841 accatcacag gcaatattgc caccacactt tcagccgggc agggcggcgg actgaatgtg
      901 tttggcggca cggcggtgtt aaacaacaat actattacat tcaacacagc cagccagagc
      961 caggcagatt cctacagcgc aggcgagggc ggcggtattt ttgccgggta ccctgtgacc
     1021 atgacgggta atctgattgc cgacaacacc gccggctatt ctcatggaaa tggtggcggc
     1081 ctgtatctga tgggcgcggc gattttgacc aataaccaga tcgtgcgtaa caccggcgca
     1141 gaccccgcca gtgaacatca gaccagctat ggcggtggac tttatctttt cgatggggat
     1201 tctgctctga taaccggtaa cctgtttgag aataaccttg ctggtgagcg cggcggcgcc
     1261 atctatctgg atgaaggctc cccgacattt agcgaaaata tcatccgggg caatcacgcc
     1321 tcaattaagg gatacggcct ggggggcggg ctgtaccttg atgaggcccc ggcgcatctg
     1381 tctaacaatc agatcatcga gaatattgcc atggatgaga atgtcggctc ggcgggtctc
     1441 agtggctttg gcggtggttt atatctggat aacagcagtg ccatcttgac cggcaacctg
     1501 atttccggca accggggcag cgtcgccacg atttatggcg aaggacgcgg cggtggggtg
     1561 tacatcctgg gcggttcccc ccaattgacc accaatcaga ttactgacaa cctggccgcc
     1621 aacgcctatt atgacaccgg ttttggcggc ggggtgtacc ttgaaagctc cggcgccaca
     1681 ttgacccgca acctgatcga tggaaacacc gccagttcca ttgataatgg ctacggcggc
     1741 ggcatctaca ccaggggatg gcacggggca gccccggttt tggatggtaa tgtcatcaca
     1801 aacaacacag ccagttttca ctcattggtc aacgatggtt ttggcggcgg ggttgccgtc
     1861 tatcgtttcg gtgagaccgt cccagtcatc ctgcgcaaca acgtcattgc caataacgat
     1921 gccgacgatt ttggcagcgg gctgtgggtc ggcggtaatt atgaaaccca gccggtgggc
     1981 gtgctgcttg agcataacac ctttgcaaat aatcaaaacg gtgatgccgc catctcactt
     2041 caatatgccc tgaccctgca aatgaccaac aatatcatct ctggccacaa cctgggcgtg
     2101 tttgccatgg aagatggtac ggttaccatg gacggcacac tctggcatca aaatacggcc
     2161 gataccggcg gcgctggcac catcacccac accaacgatc tgaccggcga tccactctat
     2221 gccgatgctg ccaacttcaa ttaccacctg acgtccggat cggctgcccg agatgccgct
     2281 cttgcatcca gcattgtatc tgatctggat ggacaggccc gccccaatcc cgataccaac
     2341 ctgccagacc tgggcgctga tgagttttac gtcacgccgc cttctgccgt gaccatctcc
     2401 gggcccggca cggcttatct gggactgccg gctaccttta ccgctcaaac cagcccggcc
     2461 gatgccacgc tccctctgac ttatacctgg caggccactg gacaggaccc ggtgacccac
     2521 cccagcggtc tggtggatac cgtccagttc acttggacag aacccggtga gcagcaaatc
     2581 accgtcattg tcaccagcgc ctatggcggg ccggttacca caaccctcat cgtgctggtg
     2641 caggtggctg tgattaaaaa agtatacctc ccgctcgttg tgcgttgaag ccgggctgtg
     2701 taagccatta aacctttccc catccattct ggagggctgt caccatcagg gggcgacagc
     2761 cctctccctt gtttgtgggc tcacacccga tgactgtccc acctcatcct tcgccgcctc
     2821 gtccccaatc tggacgagaa cgctgaccgg ttgtttcaat aattcaattt tgggcttgat
     2881 ccaattgtgt tttcgccagt gtgaggcttc cttatagtcc aacaacccat caggcatttg
     2941 attcggtaac ctggttgatg aaaacgcttg cctaaaccaa aatgatactt cttggggtct
     3001 tgacaaatct cagtattata tgttattaat aacatagtta tataataaca tatatacaaa
     3061 ttaacttata aggagtgact tgaatggacc tgcgttctac aattcttggc ttgctgaact
     3121 ggacgccttt atcaggatat gatttgaagc ggattatctc tgattcggat attttttatt
     3181 ggtctggaaa caataaccag atttataaaa cgctccttga acttgaaaaa gaggatcttg
     3241 tctcccatca agttcagctt caagagagtc tgcctgccaa aaaaatctat tccatcacga
     3301 ataaggggat ggttgccctc tatcagagct tgttggcagt ccctgagccc ccggaattac
     3361 gtaagagttt tctggttcaa ttagcgctgg cagaaagcct gtctgataaa gaaattcgga
     3421 ccttgctgga aaaatacgaa gaggaaattg cataccgtct ccgtatgtat cagggtcagg
     3481 ccgttcgcct caataacagc cccgctcgtt cacagcgaga gaaatatctc tggaagcgca
     3541 ttgctgaaaa tcttattgag acatatcaaa ctgagcttaa ttgggtgctt caaacaaagc
     3601 aggaattcag cgaacaaaaa tttttggatc tcgaacccta aacttctttg cccatattga
     3661 gctgacaacc aatccaaaat aaggagagac acaatgaata caatatccgt ctataaatca
     3721 tctgctggca aagcaaaaat catggcgctg tatgattcag tattggagcg ctggcccgta
     3781 acccacgaaa ctttcatgtt gccaacgcgg caaggtgata cttttgtaat tgccagtgga
     3841 gaaaaatcgg ccccagccct gatgcttcta cacggatctg cctccaatgc cgtttcatgg
     3901 atcggtgata tcgttgaata cagtcgtcat tttcgcgtct atgcggttga cctgccgggt
     3961 gaacctggca agagcaccga aaatcgtcct gcctgggtgg gctcagatta tgccgtatgg
     4021 ctcgaggatg ttttggatgg cctgaacata acaaaaacca gcctgctggg catctcgcaa
     4081 ggaggttgga cggccttaaa atttgcaacc atctatccac agcgcgtaaa caaattagtc
     4141 ctgctggcct ctggcggcat tctaccggcc cggtcgtcgt ttattctgaa ggctgttata
     4201 tactcaatgt ttggccattg gggttcacaa aagattatcc aaatggttaa tggaaaacag
     4261 cccatgcatc cggaagcaat tgaattcatg gaggctattt atacccattt caacacgcgt
     4321 gttgaaaaag aatacctgta ctctgatgat gaattgatac gtctgaacat gcccgtaatg
     4381 tatattggcg gtgagcagga tgcgttgttt gatacagctg ctgctgcaaa acgtctgcaa
     4441 aaactggttc ccctgcttaa ggtggttctt atcccgaaca tggggcatgc cctgatcaat
     4501 ctatcagcgg aaactctacc ctttttaacc gcatcataaa attgatactt tccttggctt
     4561 gttatccctc gcctgcctgt atgacttctg cgtcattcga cctctgtcag aacggcaggc
     4621 gccttacaac cccttgcggc tgatccgacg catgtcagcc ttgccttaca gttgctgcgc
     4681 ctgttcataa gctgccagaa gctcgcggtt ttcagccaca tttcccagga aggattggcg
     4741 cagggccgag tcatcaattt gatcagcttt ttgcatcagt gcctgataac ccgtttccaa
     4801 cacctgttgg gcctttgcgc tttggctggc tgccagaact cgatagcccg cctgataaac
     4861 aaccagcgga atcggacaac tgtcttctgg cgtttccagg cccttttcaa tctgcctgaa
     4921 taccacctca acctcagttt gagctgcatc aagctgaccc agtgccagga aagccagcgc
     4981 tatcttagaa cgggtgctca ggcattcttc tagatcgttg gtggtctcga ataattcaac
     5041 agcttgctga taatattgaa cagcctcttg gtagcgcaga tgcgcataca gcacatcccc
     5101 caggctttga taaccatagg ctttggattg gggaatttga atctcctcag ccactgccac
     5161 agctctctgt gcgtaatcaa gtgccagacc aagctcgcct tgccccaggg cagaggtggt
     5221 caggttactc aacgcgttga tctcgccgcg acgttcacca atggcgcgtc tgatctgtag
     5281 aaccttcaga aaattctcct cagcccgctg atactgtccc agataattcc aaataatccc
     5341 aatccccgac agggcaatgc tttcgccgcg ctgatcacca atgcgttgct tgatttcaat
     5401 ggcttgttcc agcagtcgca aagcatcctt ccgtcggtcc tgataattca tcaagattga
     5461 aagattaatc aatgtagcgc tctgatgcag gggatcatca atctcttcct gaatccgtaa
     5521 agccctttcg taaatttgaa tggcttggtc ataagctctc cggctgcggt aagcaatccc
     5581 cagattgact aaaaccagac tttcttggtg tcggtcgccc atttcgtggg cagcattcag
     5641 cgcttccaga taacaaccga gcgcctcatc agatccccga ttgatccggt tcagcagaat
     5701 cccca
//
LOCUS       DAOPVS010000117         5684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14155_length_5684_cov_60.025049, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000117 DAOPVS010000000
VERSION     DAOPVS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14155_length_5684_cov_60.025049"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..804
                     /locus_tag="PKL11_08715"
     CDS             25..804
                     /locus_tag="PKL11_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13722.1"
                     /translation="MATPSPASKLNCTQCGGELHPDEGQIFVTCPYCSATVYLDKTQV
                     VFHWSVAPTLSAEQAAAALARWMSGSQTVKDLDQKSRVTGQSFTYFPLWYFQVRQASG
                     QEVVLLQPGAATSVTELKNLQLPAGDLQPYNPALEAQSSEPTVPLPAARDWALQNRAD
                     TAIQTAALVHVPIYIFKYVFQNQTYTAVVEAATGRVLANLFPSKPEAPYLLAAGLTAG
                     VYLCLAALALTDSSMTGLAFIIGLIAAPILFAFAVWVANKV"
     gene            801..1838
                     /locus_tag="PKL11_08720"
     CDS             801..1838
                     /locus_tag="PKL11_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13723.1"
                     /translation="MSETDALQGLSCPRCGGMVPIPEGEAIVTCPFCELRSVVSGERG
                     VRRYQVPTRITREQAEAAYKRFLSSNLAIARSAASQAQISEVLLVHLPFWAVWGQVAA
                     WLFGYRRVKSGDSYTNVPKEERVAESMAWNAAACDVGEFGVTQISLEGRPLEAFNAEN
                     LHRTGLVFEPLGSAEEMRKAAWADFEQRARGKVNLSHISQAFMRVIRPRLGLVYYPLW
                     VVRYLYRGRSFQVVVDGYSGEALYGKAPGNSWYRAAVLVGGMALGSVLVVDVPAFIIN
                     SSEDAGMGIVISAGIGIALMYAAWRTFRHGEHYEYRRYKAGGGAPAIVSAAVKNLDGV
                     NINDVIRLLER"
     assembly_gap    1955..2054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2055..2245
                     /locus_tag="PKL11_08725"
     CDS             <2055..2245
                     /locus_tag="PKL11_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13724.1"
                     /translation="LQPPALNPGSPAAFEPVTVRPEDMRPLAEFIVLGLEAARKDKLM
                     SVEFGLDLESPELWILPA"
     gene            2303..3775
                     /locus_tag="PKL11_08730"
     CDS             2303..3775
                     /locus_tag="PKL11_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ13725.1"
                     /translation="MILYIIVPLAVLLLFLLAFMLVRTATFMETPPPPEPAASLAVEV
                     RPVAEHLSEVIRVETEDRGPGEPPAPVALTELRALLSRLYPGVHGHLQRRVMDSGALV
                     FVWTGSRHDLLPVLLAAHMDVVPIDPTTRDQWQYPPFSGEIAEGYLWGRGTLDDKGQV
                     VAILEAVENLIKTGYQPERTIYLAFGIDEEIGGEIGAGAIAAALEAEGIQLAAVLDEG
                     GGLVEGLTPGVKAPVATVGVAEKGYLSVHLTATGIAGHSATPARQTAIGALGRALARL
                     DAAQFPARFKAIETLYRELGSGASFGIQFVMANLWLLGGLVQRMTADAPNLNAIIRTT
                     IAPTIVKGGIKDNILPAQAEAVINLRLLPGDTVESALARLRRIIADERVTVEATEASW
                     DPTPTSDVDTAIYRSLSTAIRQTFPGSLVAPYLVLGATDARHYSGICTQVYRFTPYIM
                     NKEELGRVHGVNERIALDALAPMVQFYGHLVQAWGAEALE"
     gene            3840..5003
                     /locus_tag="PKL11_08735"
     CDS             3840..5003
                     /locus_tag="PKL11_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNZ13726.1"
                     /translation="MTSTVYWGSPRQARLEGKETLPAKLDLILERLNLRERVKDEYVA
                     IKLHLGNNLSYSTIHPVFVRKVIQAVKDGGGRPFVVDLDWDVAGHEQRGYSHETLGCP
                     VYPTNGLRDQYVYTRQWAYKNITEWKLGGMIHDATFIVNFAHVKGHPSCSFGAAFKNL
                     ALGSMASKTRSQMHDVHHFDRYWFSEHCPDEETRKKIIAACPFQALVEDREHPGELHL
                     HFEPCNQCGRCLKVAPAGSLVIRPENFWAFQEAMAISTKIVMDSFEPGKAVHLALATH
                     MTPVCDCFGFTSLPILPDAGIFGSDDIVAIDQAVLDMTAKTPLLEQNIPTSMEVAHHE
                     GHPFAWLHGPYKDPYKVLEYGEALGLGSRTYELVDVLPVDDISPAGLGYVPAA"
     gene            complement(5084..>5684)
                     /locus_tag="PKL11_08740"
     CDS             complement(5084..>5684)
                     /locus_tag="PKL11_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HNZ13727.1"
                     /translation="KFSFTRLLGGLLVLAALLTACTPVPAAISPAVEEPGEIQPPAPV
                     EEPTAESEVVEEEAVTTKLGPGIVPEGAVVEITAYGAPAVGDSNQRTPIAQAVSSSVG
                     LTGEADNLPEAALKELNNALAAESDGLYVVIYAGVQGSGGYGVSIESMKLNGGILEIT
                     WVLTPPKGPAITVLTHPYLIAHITGLKVTPDNVVFVQKK"
BASE COUNT         1051 a         1628 c         1789 g         1116 t
ORIGIN      
        1 accacagcat gatataatct ttttatggcc acaccctccc cagccagcaa actgaattgt
       61 acgcagtgcg gcggcgagct gcacccggac gaggggcaga tctttgtcac ctgcccgtac
      121 tgcagcgcta cggtttacct ggataagacc caggtggttt ttcactggtc ggtggcgccc
      181 accctgtctg ctgagcaggc ggcggcggcg ctggcgcgct ggatgagcgg cagccagacg
      241 gtgaaagatc tggaccaaaa atcccgcgtg acggggcaga gctttactta tttcccgctg
      301 tggtattttc aggtccggca ggccagcggg caggaggtgg tgctgctgca acccggcgcg
      361 gccacctcgg tgaccgaatt gaaaaattta cagctcccgg ctggcgacct gcagccctac
      421 aacccggcgc tggaggcgca gtccagcgag cccaccgtgc cgctgcccgc ggcgcgagac
      481 tgggcactgc aaaatcgcgc cgatacggct attcaaaccg cggcactggt gcatgtgccg
      541 atttatatct tcaagtatgt ttttcaaaat cagacctaca ccgccgtggt tgaagctgcc
      601 accgggcgag tgctggcgaa cctctttcca tccaagcctg aggcacctta tctgctggcg
      661 gcgggtctga ccgccggggt gtacctgtgc ctggcggcgc tggcgctgac cgacagcagc
      721 atgaccgggc tggcgtttat cattggcctg attgcggcgc caattctctt tgcgtttgcg
      781 gtctgggtgg cgaataaagt atgagcgaga ctgatgccct gcaaggttta agctgcccgc
      841 gctgcggcgg catggtgccc atccccgagg gtgaggcgat tgtgacctgc ccgttctgcg
      901 agctgcgctc ggtggtgagc ggcgagcgcg gggtgcggcg ctaccaggtg cccacgcgca
      961 tcacgcggga acaggctgag gccgcttaca agcgcttttt aagcagcaat ctggcgattg
     1021 cccgcagcgc tgccagccag gcgcagatca gcgaagtgct gctggtgcat ctgccgttct
     1081 gggcggtgtg ggggcaggtg gccgcgtggc tgtttggtta ccggcgcgtc aagagcggcg
     1141 acagctatac gaatgttccc aaagaggagc gcgtggctga aagcatggcc tggaacgccg
     1201 ccgcctgtga tgtgggtgaa tttggcgtca cacagatcag cctggaaggc cgcccgctgg
     1261 aagccttcaa tgccgaaaat ctgcaccgca ccgggctggt gtttgagccg ctgggttcag
     1321 ccgaagaaat gcgcaaggct gcctgggccg attttgagca gcgcgcccgg ggcaaggtga
     1381 atctgtctca catttcccag gcgtttatgc gggtcatccg cccgcggctg gggttggttt
     1441 actatccgct gtgggtggtg cgctacctgt accgcgggcg ctccttccag gtggtggtag
     1501 atggctattc tggcgaggcg ctgtatggca aggcgcccgg caattcctgg tatcgggctg
     1561 cggtgctggt ggggggcatg gcgctgggct cagttctggt tgtggatgtg ccagccttca
     1621 ttattaacag cagcgaggac gccggaatgg gcattgtgat ttcggcaggc atcggcattg
     1681 cattgatgta tgccgcctgg cgcaccttcc ggcacggcga acactatgaa taccggcgct
     1741 ataaggccgg cggcggcgcc ccggcgattg tttcggcagc cgtcaaaaat ttggatggcg
     1801 tcaatatcaa cgatgtcatc cggctgttgg agcgttaacc atgagcactg tgccggttcg
     1861 attactgccc atgttgtgcc cgcaatgtca gacgccggtg ccggcccgcc cggaagaaac
     1921 cgcctgggtc tgcgccacct gtgggcaggg gctgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnntgctgc agcccccggc gctcaacccg ggcagcccgg cagcgtttga
     2101 acccgtcacc gtgcgacctg aagacatgcg cccgctggct gagtttattg tgctggggct
     2161 ggaagccgcc cgcaaagaca agctgatgtc ggtggaattt gggctggacc tggaatcgcc
     2221 cgaactgtgg attctgcccg catagcggct tgaaatccga cctttttctt tcccctgttt
     2281 gcagttcaca attgagaaaa ccatgatcct ctacataatt gttcccctgg ctgtactgct
     2341 gctcttcctg ctggcgttca tgctcgttcg aacagccaca ttcatggaaa ccccgccgcc
     2401 gcccgaacct gccgccagcc tggcggtgga ggtgcgcccg gtggctgaac atctctccga
     2461 ggtcatccgc gtggagactg aagaccgcgg gcctggcgag ccgcccgccc cggtggcgtt
     2521 gaccgagctg cgcgccctgc tttcacggtt gtatccgggc gtgcatggtc atttgcagcg
     2581 ccgtgtgatg gacagcggtg cgctggtctt cgtctggacg ggcagccgcc acgacctgct
     2641 gccggtgctg ctggcagcgc atatggatgt ggtgccaatt gacccgacaa cccgcgacca
     2701 gtggcagtac ccgcccttct ccggtgagat tgccgagggc tacctgtggg gccgcggcac
     2761 cctggatgac aaggggcagg tggtggctat tctggaagcg gttgaaaacc tgatcaagac
     2821 cggctatcaa cctgaacgga ccatttacct ggcctttggc attgatgaag aaattggcgg
     2881 ggaaattggc gcaggcgcaa ttgccgcggc gctggaagct gaaggcattc aactggccgc
     2941 agtgctggac gaaggcggcg gtctggtgga aggcctgacg ccaggtgtga aagcgcccgt
     3001 agcaaccgtg ggggtagccg aaaagggcta cctgagcgta cacctgacgg caaccggcat
     3061 tgcaggacat tcggcgacgc cggcacggca gacggcgatt ggcgcgctgg ggcgggcgct
     3121 ggcccgctta gacgcggcgc agttcccggc acgcttcaaa gccattgaga cgctctaccg
     3181 cgagctgggc tcgggcgcca gctttggcat ccagtttgtg atggccaacc tgtggctgct
     3241 gggcggactg gtgcagcgca tgactgccga tgcgcccaac ctcaacgcca tcatccgcac
     3301 caccattgca cccaccatcg tgaagggcgg catcaaagac aatatcctgc cggcgcaggc
     3361 tgaagccgtg atcaacctgc ggctgctgcc cggcgatact gtggaaagcg ccctggcgcg
     3421 gctgcgccgc atcattgctg atgagcgggt gacggtggaa gccaccgagg cgagttggga
     3481 ccccacgccg acatccgatg tggatacagc catctatcgc agcctttcga cagccatccg
     3541 gcagactttc cccggatcgc tggtggcgcc gtaccttgta ctgggcgcga ccgatgcccg
     3601 gcactattcc ggcatctgca cgcaggtgta ccgctttacc ccttacatca tgaataagga
     3661 agagctgggt cgcgtgcatg gcgtgaacga gcgcattgcg ctggatgccc tggcgcccat
     3721 ggtgcagttc tacgggcacc tggtgcaagc ctggggcgcc gaggcgcttg aatagacccg
     3781 tttctggaag aaatgttcca aaacgatttg ctctaattta cccgtaaagg agattgacca
     3841 tgaccagcac tgtttactgg ggctcgcccc gtcaggcccg gctggaaggc aaagaaaccc
     3901 tgcccgccaa gctcgatttg attctggaac ggctgaacct gcgagagcgg gtcaaagatg
     3961 aatatgttgc catcaaactg cacctgggca acaatctgtc ttattccacc atccacccgg
     4021 tgtttgtccg caaagttatt caggccgtta aagacggcgg cgggcggccc tttgtggtgg
     4081 acctggattg ggatgtggcc gggcatgagc agcgcggcta cagccacgaa actctgggct
     4141 gcccggtgta ccccaccaac gggctgcgcg atcagtacgt ctatacccgg cagtgggctt
     4201 acaagaacat caccgagtgg aagctgggcg gcatgattca tgacgctacg ttcatcgtca
     4261 actttgctca cgtcaagggg cacccgagct gcagctttgg ggcggccttc aagaacctgg
     4321 cgctgggcag catggccagc aagacgcgca gccagatgca cgatgtgcat cactttgacc
     4381 gctactggtt cagcgaacac tgccccgacg aagaaacccg caagaagatt atagcggcct
     4441 gccccttcca ggcgctggtg gaagaccggg agcaccccgg cgagctgcac ctgcactttg
     4501 aaccgtgcaa tcagtgcggg cgctgcctga aggtggcccc ggcgggcagt ctggtgatcc
     4561 gcccggagaa cttctgggcc ttccaggaag ccatggcgat cagcaccaag attgtgatgg
     4621 acagctttga accgggcaag gcggtgcacc tggcgctggc gacgcatatg acgcccgtct
     4681 gtgactgctt tggctttacc agcctgccca tcctgccgga tgcgggcatc ttcggctcgg
     4741 atgatattgt ggcgattgat caggctgtgc tggatatgac cgccaaaacc ccgctgctgg
     4801 agcaaaatat tccgacatca atggaagtgg cccatcacga ggggcatccc ttcgcctggc
     4861 tgcatgggcc atacaaagat ccctacaagg tgctggaata tggcgaggcc ctggggctgg
     4921 gcagccgcac ctatgaactg gtggatgttc tgccagtgga tgatatttca cccgcagggc
     4981 tggggtacgt accggctgct taatgcggaa gttgaggcaa aaataatcaa agagccgcgc
     5041 cggtatgggc gcggctcttt gatatcaacc ggcagccgcc gggctatttc ttctgcacaa
     5101 aaacgacgtt atccggcgtc actttgaggc cggtaatgtg ggcaatcaga taggggtgcg
     5161 tcagcacggt gatggccggg cctttgggcg gcgtgagcac ccaggtgatt tccaggatgc
     5221 cgccgttcag cttcatggat tcgatggata cgccgtagcc gccgctgccc tgcacaccgg
     5281 cataaatgac cacatagagg ccatctgatt ctgccgccag ggcgttattc agttctttga
     5341 gggcggcttc gggcaggttg tcggcttcgc cggtcagacc caccgagctg gacacggcct
     5401 gggcaatcgg cgtccgctgg ttgctgtcgc cgacagccgg ggcgccgtag gcagtgattt
     5461 caacaacggc accttcgggc acaattccag ggcctaattt ggtggtcaca gcttcctcct
     5521 ctacaacttc tgattctgcc gtgggttctt caaccggggc agggggttga atctcaccgg
     5581 gttcttcaac cgcgggagaa atagctgccg gcacgggggt gcaggcggtc agcagtgcag
     5641 ccagcacaag taaacctccg agcaggcggg taaaagaaaa tttc
//
LOCUS       DAOPVS010000118         5646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14286_length_5646_cov_57.181542, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000118 DAOPVS010000000
VERSION     DAOPVS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14286_length_5646_cov_57.181542"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2784
                     /locus_tag="PKL11_08745"
     CDS             <1..2784
                     /locus_tag="PKL11_08745"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNZ13728.1"
                     /translation="IAYLGEQSRRPVSCIPNAGLPLNVDGQAVYPLEPEPFAETLAEF
                     VERNRINVVGGCCGTTPAHLKRLVERLRGQSSPERPTLELPRLASSVQAMDMQQEPRP
                     FLIGERLNTAGSAKFKKLILADDFEGALTIAQQQVESGAHALDLCVALTERSEETALM
                     TRLIKTLAPTTRVPFVIDSTEPDVMEAALKAAPGRCLINSTHLEGGRAKAERVFGLAR
                     QYGAAIICLTIDEQGMAKTAERKLAVARRIYEMATVEFGLRPGDLVFDALTFTLATGE
                     AEFASAAVETMEGIRRIKAELPGVLTSLGVSNCSFGLSAAARAVLNSVMLYHAVQAGL
                     DMAIVNPAQINPYADIPTEERALAEDLIFNRRPDALTRLIAHFEQVKSTPGSEHSQAA
                     FDAMRPEERLHWCILHRQREGVEEAIEEILSRESAASRGENAVHILNTVLLPAMKTVG
                     DKFGSGELILPFVLQSAEVMKRAVAYLEQYLERKAGVSKGTVVLATVYGDVHDIGKNL
                     VKTILSNNGYTVVDLGKQVPAETIITQAVELKADAIGLSALLVSTSKQMPLIVNELQR
                     RGLNFPVLIGGAAINQRFGRRILLTETGEFYAPGVFYCKDAFEGLNTMDALVDAQQRP
                     ALLERIRRESEVELGRITPGRVEPAAGKRSAVSPALAIPRPIEWGVRVVRSMPLEAVF
                     ACLNKNELFRLSWGAKNTHGEAWTQLQAEYEVRLDEMRRAALKESWLKPQGIYGYWPA
                     QADGNDLIIYDPASVEVGRPTEIVRLNFPRQPGGEYLCLADYFMPVDSKMMDVAALQV
                     VTVGAEATERFDRLQAAGEYSAAYFSHGLAVQMAEAAAEYLHQHVRRELGLAAGQGKR
                     YSWGYPSIPELGDHEKVFALLPARRELGMSLTPAYQLVPEQSTAAIILHHPQARYFNI
                     GESRVDQLMGSAS"
     gene            2845..4725
                     /locus_tag="PKL11_08750"
     CDS             2845..4725
                     /locus_tag="PKL11_08750"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HNZ13729.1"
                     /translation="MTTTAFLDLLQQPRPILSDGAMGTLLHQRGVNFEQCFDLLNLSN
                     PAMVAAIHREYIEAGSNMIQTNTFSANRFKLAQHGLEKQVAEINTAGVELARRIVLAS
                     FRDVLIVGDVGPLGVRLAPFGRVQPEQARLAFIEQIGALADAGVNLLIIETMTDLYEV
                     NEAIAAARHVAPQLPIIASMTFTRDDRTLLGDNPAKVARYLQQAGADVIGVNCSGGPS
                     QILSILRQMRQAAPDIHYSVMPNAGWPQQASGRIMYPAGPEYFGEYALAFWQEGAKVI
                     GGCCGTTPGHIEAMRRAVDAAPATMAEPAFSLAVVEREEAQAAQPPTQLAEKLATGQF
                     VIAAEMDPPRGLSMHKLLAGASLLSDAGADVIDVADSPMARMRMSPWAVCSLIQKQVG
                     VETVLHFPTRGRNLLRVQGDLLAAHALGVRNVFVVMGDPTAIGDYPDAMDNYDLVPSG
                     LIKLIKQGFNEGVDHAGVDIGQPTSFFAGCALNLTPNNLTEEIKNLRRKLNAGADFIL
                     TQPVFDPSKAVESLCAIREALDGLRVPVLVGILPLASARHAAFLHHEVPGILIPDSVR
                     ARMDNAGENAARAGIEIAIELVEQMRAATSIQGIYIMPAFSRFDYAAEVIEAVRFSEV
                     RA"
     gene            4746..5528
                     /locus_tag="PKL11_08755"
     CDS             4746..5528
                     /locus_tag="PKL11_08755"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNZ13730.1"
                     /translation="MLLTIDIGNTNLTFGLYDNATLGPHWRLATDHERMPDEYGLQMM
                     GLLQSIGRTPEELSGICLSSVVPQLTGRILSACQQYLNQEPLVVDSSLKTGITILYED
                     PRAVGADRIADAVAVQQIYGGPACIIDFGTATTFNALTGEGEYLGGAITAGVGVAADA
                     LFKRAARLSPVELVAPPSVIGRNTVHAVQSGLVFGYVALVEGMTARFRTILGERMKVI
                     ATGGLAEVIATHTPAIDIIAPWLTLDGLRIIYEINTGGEVKP"
     gene            5525..>5646
                     /locus_tag="PKL11_08760"
     CDS             5525..>5646
                     /locus_tag="PKL11_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNZ13731.1"
                     /translation="MNPIAGKRIILGVTGSIAAYKAADLASKLHQAGALMDVILT"
BASE COUNT         1203 a         1446 c         1887 g         1110 t
ORIGIN      
        1 attgcctacc tgggcgagca gagccgccgc ccggtatcgt gcataccgaa tgcagggctg
       61 ccgctgaatg tggatgggca ggcagtgtac ccgctggagc cggagccatt tgccgagaca
      121 ctggcagagt ttgtggagcg caaccgcatc aatgtagtgg gaggctgctg cggcaccacc
      181 ccggcacacc tcaaaaggct ggtggaacgg ctgcgcgggc aaagctcgcc agagcgccca
      241 accctggaac tgccgcggct ggcttcatcg gtacaggcca tggatatgca gcaggagccg
      301 cgcccgttcc tgattgggga acggctgaac acagccggca gcgccaagtt caagaagctg
      361 attctggcgg atgattttga gggcgcgctg acgattgccc agcagcaggt tgagagcggg
      421 gcacatgcgc tggacctgtg cgtggcactg accgagcgct cggaagaaac cgccctgatg
      481 acccggttga tcaaaacgct ggcgccgact acgcgggtgc cctttgtgat tgactcgacc
      541 gagccggacg tgatggaagc cgccctgaaa gcagcgccgg gccgctgtct gattaactcc
      601 acccatctgg aaggtgggcg cgccaaagca gaacgcgttt ttgggctggc gaggcagtac
      661 ggcgcggcga tcatctgcct gacgattgat gaacagggga tggctaaaac cgctgaacgc
      721 aagctggcgg tagcccgacg gatttatgag atggcaacgg ttgaatttgg gctgcgacca
      781 ggcgacctgg tatttgatgc gttaaccttc acgctggcaa ccggcgaggc tgaattcgcg
      841 tctgcggcag tggaaacaat ggaaggtatc cggcggatca aagctgaact gccgggcgtg
      901 ctgacctcgc tgggggtgag caactgctcg tttgggcttt cggcagcggc gcgggcggtg
      961 ctgaacagcg tgatgctgta tcacgcagtg caggcagggc tggacatggc cattgtcaat
     1021 ccagcgcaga ttaacccgta tgccgatatt cccaccgaag aacgagcgct ggcagaagat
     1081 ttgatcttca accggaggcc ggacgcgctg acacggctga tcgctcattt tgagcaggtg
     1141 aaaagtaccc ccggcagcga acacagccag gcagcgtttg acgcaatgcg cccggaagaa
     1201 cggctgcact ggtgcattct gcaccggcag cgcgagggtg tggaggaagc cattgaggag
     1261 attctaagcc gcgagagtgc ggcatcgcgc ggggagaatg ccgtgcatat tctcaacacg
     1321 gtcttactgc cagcgatgaa aaccgtgggc gataaattcg gcagcggcga gttgattctg
     1381 ccgtttgtgc tgcaatcggc tgaagtgatg aagcgggcag tggcgtattt ggaacagtac
     1441 ctggagcgca aagcgggagt gagcaagggt accgtggtgc tggcgacagt ttatggagac
     1501 gtgcatgaca tcggcaaaaa tctagtcaag acgattctct ccaacaacgg ctatacggtg
     1561 gtagacctgg gtaagcaggt gcccgccgag acgatcatca cccaggccgt ggaactaaag
     1621 gcggatgcca tcgggctttc ggcgctgctg gtgagcacca gcaagcaaat gccgctgatt
     1681 gtgaacgaac tacagcggcg gggattaaat ttcccggtgt tgattggggg ggcagcgatt
     1741 aatcaacggt ttgggcgacg gattctgcta accgagaccg gcgaatttta cgcgccgggt
     1801 gtgttttact gcaaagatgc gtttgaaggg ctcaatacaa tggatgcgct ggtagatgca
     1861 cagcaacgcc cggcattact ggaacggatc cggcgggagt ctgaggtgga gctggggcgc
     1921 atcactcccg ggcgagtgga gccagccgcg ggcaaacgct cagcggtcag cccggcgctg
     1981 gcgattccac gcccgattga atggggtgtt cgggtggtgc gctcgatgcc gctggaagcg
     2041 gtatttgcct gcctgaacaa gaatgaactc ttccgattgt cgtggggggc caagaatacc
     2101 cacggcgaag cctggacgca gcttcaggct gaatacgaag tgcggctgga tgagatgcgc
     2161 cgcgcagcgc tgaaagagag ttggctgaag ccgcagggga tttacggcta ctggcccgcc
     2221 caggccgatg gcaatgacct gatcatctac gacccggcat cggttgaggt ggggcgcccg
     2281 acagagattg tgcggctgaa ttttccccgg cagcccgggg gtgaatacct gtgcctggca
     2341 gattacttca tgccggtaga ttcaaagatg atggacgtgg cggcgctgca agtggtgacg
     2401 gtgggcgctg aagccaccga acgctttgac cgactgcaag ctgcaggtga gtacagcgcg
     2461 gcgtattttt cgcatgggct ggcagtgcaa atggccgagg cggcagcaga atacctgcat
     2521 caacatgtgc ggcgggagtt gggattggcg gctggccagg gtaagcgcta ttcgtggggg
     2581 tatccttcca tcccggaatt gggcgaccac gagaaggttt ttgcgctgct gcccgcccgc
     2641 cgtgagctag gcatgagctt gacgccagct tatcaactgg tgccggagca atcgacagca
     2701 gcgattattc tacatcaccc acaggcgcgc tatttcaata ttggcgagag ccgggtggat
     2761 cagttgatgg gaagcgcaag ttgaaaagca gacggaagcc tgcaagccaa gcgcgtggcg
     2821 ctccagaaat gatgaggtaa gaagatgaca acgacagcct ttctcgattt actacaacag
     2881 ccccgcccga ttctatcgga tggagccatg gggacgttac tgcatcaacg cggggttaat
     2941 tttgagcagt gttttgattt gctgaacctg agcaatccgg cgatggtggc agccatccac
     3001 cgtgaatata ttgaagccgg gtccaatatg attcagacga atacgttcag tgccaaccgg
     3061 ttcaaactgg cccagcatgg gctggaaaaa caggtcgcgg agatcaacac tgccggggtg
     3121 gaactggcga ggcgaatcgt gctggcatcc ttcagagatg tgctaattgt gggagatgtg
     3181 gggccgctgg gtgtgcggct ggcgccgttt ggacgggtgc agcctgaaca ggcgcggctg
     3241 gcatttattg aacagattgg ggccctggcc gatgccgggg ttaacctgct gatcattgag
     3301 accatgaccg atctgtatga agttaatgag gcgattgcag cggcccggca tgtggcaccc
     3361 caactgccaa tcattgcgtc catgaccttc acgcgtgacg accgcacgct gctgggtgat
     3421 aaccccgcca aagtagcgcg gtatctgcag caagccggcg cggatgtgat tggggtgaac
     3481 tgctcgggcg gaccaagcca aattttatcc attttgcggc agatgcggca ggcagcgccg
     3541 gatatccatt actctgtgat gccaaatgcc ggctggccgc agcaggcctc tgggcggatt
     3601 atgtacccgg cgggacctga atattttggc gaatatgcgc tggcgttctg gcaggaaggc
     3661 gccaaagtga ttggcggctg ctgcggcacg acgccggggc acattgaagc catgcggcgg
     3721 gcggtcgacg cggcaccggc aacgatggct gaacccgcat tcagtctggc agtggttgag
     3781 cgcgaagagg cacaggccgc gcagccgcct acgcaactgg ccgaaaagct ggcgacgggg
     3841 caatttgtga ttgcagcaga aatggacccg ccccgcgggc tttccatgca caagctgctg
     3901 gcgggcgcca gcctgctgag cgatgccggc gccgatgtca ttgacgtggc agacagccca
     3961 atggcgcgga tgcggatgag tccatgggca gtgtgcagcc tgattcaaaa acaggtgggg
     4021 gtggagacgg tgctgcactt tcccacccgg ggacgcaacc tgctgcgggt gcagggcgac
     4081 ctgctggcgg ctcacgcgct gggcgtgcgc aatgttttcg tggtgatggg tgacccaacg
     4141 gcaattggcg attacccgga tgcaatggat aattatgacc tggtgccatc aggcttgatt
     4201 aagctaatca agcaaggatt taatgaaggc gtggaccatg cgggcgttga tattgggcag
     4261 cccacatcgt tttttgcggg ctgcgcgctc aacctgacgc ccaataatct gacggaggag
     4321 attaaaaatc tacggcgcaa gctgaatgcc ggagccgatt ttatcctgac acagccggtt
     4381 ttcgatccat cgaaggcggt tgaatcgtta tgcgccatcc gagaagcgct ggatggtttg
     4441 cgtgtgccgg ttctggtggg tattctgccg ctggcatcgg cgcggcacgc ggcttttctg
     4501 caccacgaag tgccggggat tttgatcccg gacagtgtgc gggcccgtat ggataacgct
     4561 ggagaaaatg cggcgcgcgc gggaatcgaa attgcgatag aattggtgga gcagatgcgc
     4621 gcagccactt ccatccaggg catatacatt atgccggctt tcagccggtt tgattatgca
     4681 gcagaagtga ttgaggctgt gcggtttagt gaggtgcgcg cgtaaacttc tgctggagat
     4741 aagccatgct gctgacaatc gatataggca acaccaacct gacctttggg ttgtatgaca
     4801 atgccacact ggggccacac tggcggctgg caacggacca tgagcgcatg ccggatgaat
     4861 atggcctgca aatgatgggg ctgctgcaat ccattgggcg aaccccggaa gagctgagcg
     4921 gtatctgcct gtcttcggta gtgccgcagt tgaccgggcg gattttatcg gcctgccagc
     4981 agtacctgaa ccaggaacca ctggtggttg attcgagcct gaagacggga attacgattt
     5041 tatacgaaga cccgcgggcg gttggcgctg accggattgc cgatgctgtg gctgtgcagc
     5101 agatttacgg cggtccggcg tgtattattg attttggcac ggcaacaacc ttcaacgcgc
     5161 tgacaggaga aggggaatac ctgggcgggg cgatcaccgc gggggtgggc gtggcagccg
     5221 atgcgctgtt caaacgcgcc gcgcggctct cgccagtgga actggtggca ccgcccagcg
     5281 tgattggacg caacacggta cacgcggttc agtccgggct ggtgtttgga tatgttgcgc
     5341 tggtggaagg gatgaccgcc cggtttcgga cgattctggg cgagcggatg aaagtgattg
     5401 ccacaggtgg gttggccgag gtgattgcca cccatacacc ggcgattgac attattgcac
     5461 cctggctgac gctggacggg ctgcgaatta tctacgaaat aaacacaggc ggggaggtga
     5521 aaccatgaat ccgatcgcgg ggaaacgaat catcctgggg gtaactggct caatcgcggc
     5581 ttataaggct gctgacctgg cctccaagct gcatcaggcc ggggcgttga tggatgtcat
     5641 cctcac
//
LOCUS       DAOPVS010000119         5628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14340_length_5628_cov_41.194509, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000119 DAOPVS010000000
VERSION     DAOPVS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14340_length_5628_cov_41.194509"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PKL11_08765"
     CDS             <1..1283
                     /locus_tag="PKL11_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ13732.1"
                     /translation="VIPADLVSFWSEVEVEALSDTAVQFRLPEPFAPFVDYLSFGILP
                     QHIFGQFSFAEMIDSPANLAPIGSGPYQFDRLIVENGQIMGVALQVNEDYYLDLPNIA
                     EFIFRYYPDSASALQAYQDDVVQGVASISADALNAALAEPDLALYTGRKPELALVLFN
                     LNNSEVDFFQNAVVRQALYAGLNRQFMVDRVLNGQAILADGVILPGTWAYHEGLGQVA
                     YDPNTALQMLKEEDYILADENATVRSKEDKLLQFTLIYPDTVQHRAIAERIQADWKSL
                     NVGVILEAVPYDQLIQDRLASHNYQAALVDLNLSRSPDPDPYPFWDQVQATGGQNYSQ
                     WNNNMASEHLENARVAVDISERMRLYNNFQAIFADELPALPLYYPVYTYAVRNDVQGV
                     RMGPLFDSCDRFASVLDWYLLAPQEQLPAATATP"
     gene            1316..1858
                     /locus_tag="PKL11_08770"
     CDS             1316..1858
                     /locus_tag="PKL11_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13733.1"
                     /translation="MLLETDWAFISASLEELEAYLKSKQVYWPLSGPASLPRLTLGSL
                     LVSLARLEAHAITEVEPARVSAMLADVSSIRSHWQALWQCKAALEFPQRLTLWSAFLS
                     DLKEEGVSRAEYAAQVHLRLMLDLLLAEPPTIQPAWPRQLEALDVRLQTVTRPAPFVW
                     GAELEPGFPPDKFWYLYRGW"
     gene            1862..2413
                     /locus_tag="PKL11_08775"
     CDS             1862..2413
                     /locus_tag="PKL11_08775"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNZ13734.1"
                     /translation="MSQFYTRRGDDGTTGVLGEGRVPKYDLRMEALGAIDEATAALGT
                     ARAQSNLQLADLLLHLQRALYRLMAEVAATPENAERFLSIDAAQVQWLEARTDEWAAQ
                     VNLPREFIVPGDTPLGAALALSRTIVRRAERRVAELLARGDIANANLLAYLNRLSSLC
                     FVLELYANQSAGAAPTLAKNKAG"
     gene            2413..3111
                     /locus_tag="PKL11_08780"
     CDS             2413..3111
                     /locus_tag="PKL11_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNZ13735.1"
                     /translation="MGTALNVIAILVGGALGLLLGPRLPDRVRQTVVAGMGLFTLAYA
                     LKQFFTTANALLVLGAILIGALLGEWWQIEAGMARLGAWLERKFSPGSVDGQAASRRF
                     VRGFLSASLIFCIGPMAILGSIQDGVTGDFSTLAIKSILDGFIALAFASSLGVGVLFS
                     ALPILVYQGSITLLAAQVQAVVTPAMMTEMTATGGIILMGVAVSSLLEIRQIRAGNFL
                     PALIIAPILAAIFL"
     gene            complement(3125..3475)
                     /locus_tag="PKL11_08785"
     CDS             complement(3125..3475)
                     /locus_tag="PKL11_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNZ13736.1"
                     /translation="MSTRLDEFEAYRTRMNERVMQIDHLGIKRFFNLDTAAYHDGALS
                     GKEKELLGLVASMVLRCNDCIDYHILQCVKAGYSDAELYEAFNVALIVGGSIVIPHLR
                     HAVESLDLCREREG"
     gene            complement(3472..4344)
                     /locus_tag="PKL11_08790"
     CDS             complement(3472..4344)
                     /locus_tag="PKL11_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13737.1"
                     /translation="MIFRRPISIVRPAAAKDRAALSELISLSAHVHRHLDWRPPLDWL
                     DSQPFLVLERDETLLAALACPAEPPQVAWLRLFVVHPRMQPNQAWEVLHAEAVRALPL
                     PRPQICAVALQTWISELLHRQGFIHHQDIVTLEWSCQLKPLYLPEGIHIRPMVYEDLG
                     DVAEIDQSAFEEIWRNRLVDVTRAYYQSAYATVAVDANGAILGFQISTSNGPQAHLAR
                     LAARPHCQRRGIGVALVAELQQHFAREGFYIVSVNTQSTNQASLALYQKVGFVQTGER
                     FPVWIDSTPAEETK"
     gene            complement(4356..4901)
                     /locus_tag="PKL11_08795"
     CDS             complement(4356..4901)
                     /locus_tag="PKL11_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13738.1"
                     /translation="MAENYDLQTLIKQAKEAYEAEEYLQAAQGFEAAAHASELSGDAL
                     NAAEMQNNRSVALLKARNPEAALEAARGTDLIFAQAGDLRRQGMALGNQAAALDDLRR
                     DEESIAAYQQSAALFEQAGEKDMRALVLRRVSAIHYRQKNTPAALSTMLDALNCQTRL
                     SLQDRILKGLLKIVARLSTRG"
     gene            complement(4913..5593)
                     /locus_tag="PKL11_08800"
     CDS             complement(4913..5593)
                     /locus_tag="PKL11_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ13739.1"
                     /translation="MSANDKVLQIRTRKLGVLIKDARMAAKRSVEECARAMGIQPETL
                     RQYEAGAKPPSLPDLEALAYYLDIPLEHFWSAQTLSGVDNAAKVEQIRQLNKIRNRII
                     GTTLRANRTRRNVAVKDLAEQTDIPEPQLRRYEAGEIPIPLPELEILAAALDTRVEDF
                     FDSRGPIGNWRAQQQAVDKFMELPGDLRDFVCRPVNRPYLDLAMRLSALSVDRLRSVA
                     EGLLEITY"
BASE COUNT         1079 a         1742 c         1491 g         1316 t
ORIGIN      
        1 acgttatccc tgcagacctg gtgtccttct ggtctgaggt cgaagtcgaa gccctcagcg
       61 ataccgctgt gcagttccgt ctgccagaac cctttgcccc ctttgtagac tatctctcct
      121 tcggcatctt gccccagcac atcttcggtc aattctcatt tgccgagatg atcgattcgc
      181 ccgccaatct ggcccccatc ggcagcggtc cctaccaatt tgaccgtttg attgttgaaa
      241 acggccagat catgggcgtt gcccttcagg tgaacgaaga ctattacctt gacctgccga
      301 acatagccga attcatcttc cgctattacc ccgactctgc ttccgccttg caggcctacc
      361 aggatgatgt cgttcagggt gttgcctcga tctctgccga tgcgctcaac gccgccctgg
      421 ccgagcccga cctggccctt tacactggcc gcaagccgga actggcgctg gtacttttca
      481 acctcaacaa ctcagaggtc gatttcttcc agaatgcggt tgtccggcaa gcgttatatg
      541 cgggcctcaa tcggcagttt atggttgacc gtgtgcttaa tggtcaggcc attctggccg
      601 atggggtgat cctgccggga acctgggcct atcacgaagg tctgggacag gtagcctacg
      661 accccaacac cgcccttcag atgttgaaag aagaagacta tatcctggca gacgagaacg
      721 ccaccgttcg atccaaggaa gacaagcttc tccagttcac cctgatctat cccgatactg
      781 tccagcaccg cgccattgcc gagcgcatcc aggccgactg gaaaagcctg aatgtcggcg
      841 ttatcctcga agccgtcccc tacgaccagc tcatccagga tcgccttgcc agccacaatt
      901 atcaggccgc cctggtcgat ttgaacttgt cccgctcacc cgatcctgac ccttacccgt
      961 tctgggatca ggttcaggcg actggcggtc aaaattattc ccaatggaat aacaacatgg
     1021 ccagtgagca tctggaaaac gcccgcgttg ctgtagatat cagcgaacgg atgcgcctct
     1081 ataacaactt ccaggcaatc tttgccgacg aactgcccgc gctgccgttg tattatcccg
     1141 tctataccta cgccgtgcgc aatgatgtgc agggtgtgcg catgggtccg ctctttgact
     1201 catgcgaccg ctttgcctcc gtactggatt ggtatctgct cgcgccgcag gaacaacttc
     1261 ccgcggccac cgctaccccg tagttctggc gctgccggtt gccagaggag ggctcatgct
     1321 gctggaaacc gattgggcgt ttatctcggc ttcccttgaa gaactggaag cctatctcaa
     1381 gtcaaaacag gtttactggc cgctctccgg accggccagt ttgccgcgcc tgactctggg
     1441 cagcctgttg gtctcgctgg ctcgcctgga agcccatgcc attactgagg tggagcctgc
     1501 ccgtgtatcg gccatgctgg cagatgtgtc gtcaattcgc tcacattggc aggcgctctg
     1561 gcagtgcaaa gcagccctgg aatttcccca gcgcctcacc ctctggtcgg ctttcctgtc
     1621 cgatctcaaa gaagaaggcg tttctcgggc agaatatgcc gcccaggttc acctgcgcct
     1681 gatgcttgat ctcttgctgg cagaaccccc caccattcag cccgcctggc cgcgccagtt
     1741 ggaggccctg gatgtgcgtt tgcagactgt gacccgcccg gcaccatttg tctggggggc
     1801 agaattggaa cccggtttcc cgccggataa attttggtat ctttatcgag gatggtaacc
     1861 catgagtcaa ttttataccc gtcgcggtga tgacggcaca accggtgttc ttggcgaggg
     1921 ccgcgtccct aaatatgatc tacgtatgga ggccctgggc gccattgacg aagcaaccgc
     1981 cgccctggga acggcccgcg cccaatctaa cttgcagctg gccgatttgt tgctgcactt
     2041 gcagcgcgcc ctttaccggc tgatggctga ggttgctgcc acgcctgaga acgccgagcg
     2101 ctttttatcc attgacgcgg ctcaggtcca gtggctcgag gcccgcacag atgaatgggc
     2161 cgctcaggtc aatttgcccc gtgaatttat cgtccccggc gacaccccgc ttggagctgc
     2221 tttagccctt tcgcgcacca tcgtccgccg ggcagagcgc cgggttgccg aactgctggc
     2281 tcgcggcgat atcgccaatg ccaacttgct ggcttatctc aatcggcttt cttcgctctg
     2341 cttcgtgctg gagctgtatg ccaaccaatc cgctggtgcc gcaccaaccc tggcaaagaa
     2401 taaggcgggt tgatgggtac tgctctcaat gtgattgcca tcctggttgg cggcgctctg
     2461 ggactgcttt tagggccgcg cctgcccgac cgcgtccgtc aaacggttgt tgctggcatg
     2521 ggcctcttta ccctggctta tgccctcaaa caattcttca ccaccgccaa tgccctgctg
     2581 gtcctgggcg ctattctgat tggcgccttg ctgggcgaat ggtggcaaat tgaggccggt
     2641 atggcccgtc tgggtgcctg gctggaacgc aaattctcgc ccggctctgt cgatggtcag
     2701 gccgccagca gacggtttgt ccgcggcttc ctctctgcct cgttgatttt ttgcatcggc
     2761 cccatggcga tcctgggctc cattcaagat ggcgtcaccg gagatttttc aactctggcg
     2821 atcaaatcaa ttctggatgg ttttattgct ctggcctttg cctccagcct gggggtgggg
     2881 gtgctgtttt ctgcactgcc catcctggtc tatcaaggct caatcaccct gctggcggct
     2941 caggttcagg ccgtcgtcac ccccgccatg atgaccgaaa tgaccgccac tggcggcatc
     3001 attctgatgg gggttgccgt cagcagcctg cttgaaattc gccaaatcag ggctggcaac
     3061 ttcctgcccg ctctgatcat tgcacctatt cttgctgcaa tttttcttta accgctgcgc
     3121 tcggctagcc ttcccgctcc cggcataaat ccagcgattc aaccgcatgg cgtaaatgcg
     3181 gaatgacaat gctgccgccc acaatcagtg ccacattgaa ggcctcgtaa agctctgcgt
     3241 cgctgtagcc cgccttcacg cactgcaaga tatgataatc aatgcagtca ttgcagcgca
     3301 gcaccatgga tgccaccagc cccagcagct ctttttcttt tccgctcagc gcaccgtcat
     3361 ggtacgctgc cgtatccaga ttgaagaagc gcttaatgcc caggtggtcg atctgcatca
     3421 cccgctcatt catccgggtg cggtaagctt caaattcatc caatcgtgtg ctcatttcgt
     3481 ttcctccgca ggggttgaat cgatccatac cggaaaacgc tcaccggttt gtacaaaacc
     3541 aaccttttga tataacgcca gcgaagcctg attggtgctc tgggtattga cgctgacaat
     3601 ataaaagcct tcacgagcaa aatgctgttg aagctccgcc accagtgcta caccaatccc
     3661 gcgccgctga caatggggcc gcgccgccag ccgcgccagg tgtgcctgag gcccgttgct
     3721 ggtgctgatt tgaaaaccca aaatggcgcc attggcgtca actgccaccg tggcataggc
     3781 gctttgataa taggcccgag tcacatctac cagccggttg cgccagattt cctcaaacgc
     3841 actttgatca atctccgcca cgtctcccag gtcttcatac accatcgggc gaatatggat
     3901 gccctccggc aggtacaggg gttttagctg gcaggaccat tccagggtca caatgtcctg
     3961 gtgatggata aatccctgcc ggtgcagcag ctcactgatc caggtctgca gcgccaccgc
     4021 gcagatttgg gggcggggca gcggcagcgc ccgcacggcc tctgcatgca gcacttccca
     4081 ggcctgattg ggctgcatgc gcgggtgaac cacaaacagc ctcagccagg ccacctgcgg
     4141 tggttctgcc gggcaggcca gcgccgccag caaggtctcg tcgcgttcca aaaccagaaa
     4201 cggctggctg tccagccaat ccagcggggg gcgccaatcc agatgccgat gcacatgcgc
     4261 gctcaggctg atcaattcag acagcgcagc ccggtctttg gcggctgcgg gtcgcacaat
     4321 ggaaatgggg cggcgaaaaa tcatggccgc cgcgtttatc cccgggtgct caaccgggca
     4381 acaattttca ataacccttt gagaattcga tcctgcaggc tcaaacgggt ctggcagttc
     4441 agtgcatcca gcatggtgga gagcgccgca ggcgtgtttt tctggcggta atgaattgcc
     4501 gaaactcgcc gcagcaccaa cgcccgcata tctttttcgc cagcctgctc aaacagcgcc
     4561 gccgattgtt gatacgcagc aattgattcc tcatcccggc gcagatcatc cagcgccgcc
     4621 gcctgattgc ccagcgccat accttgccgg cgtaaatcac ctgcctgcgc aaaaatcagg
     4681 tcggtcccgc gggcggcttc cagcgcagcc tcgggattgc gggcttttag cagcgccaca
     4741 ctgcggttat tttgcatttc cgccgcgtta agtgcatcgc ccgaaagttc gctggcatgg
     4801 gcagccgcct caaaaccttg cgcggcctgc aaatattctt ctgcttcata ggcctctttg
     4861 gcctgtttga taagcgtttg taaatcgtaa ttttcagcca tatgattcca tcttagtaag
     4921 tgatttcgag taagccttca gctaccgagc gcaacctatc aaccgacagc gcgctgagcc
     4981 gcatggctaa atctaaataa ggtcggttga ccggtcggca gacaaaatcg cgcaggtcgc
     5041 cgggcagctc cataaactta tccaccgcct gctgctgtgc gcgccagttt ccaatcggtc
     5101 cgcgtgaatc aaagaaatct tctacgcgcg tatccagtgc cgccgccaga atttccagct
     5161 ccggcagtgg gatgggaatt tccccggctt catagcgccg cagttggggc tctggaatat
     5221 ccgtctgttc agccaggtct ttcacggcca cattgcgccg ggtgcggttg gctcgcagcg
     5281 tagtgccaat aatccggttg cggattttgt tcaactgccg aatctgctcc acctttgccg
     5341 cattatccac cccagacagg gtctgcgcac tccaaaaatg ttccagcgga atatccaggt
     5401 aatacgccag cgcctccagg tcgggcagtg agggcggttt ggcaccagct tcatattgac
     5461 gcagggtttc tggctggatt cccatggcgc gggcacattc ttccacgctg cgtttggccg
     5521 ccatccgggc atccttgatc aagaccccca gcttgcgtgt tcggatttgt aagactttgt
     5581 cgtttgcgct catggttttc cgtctccgtt aacctattat agcaaagt
//
LOCUS       DAOPVS010000120         5623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14362_length_5623_cov_50.751796, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000120 DAOPVS010000000
VERSION     DAOPVS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14362_length_5623_cov_50.751796"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..851)
                     /gene="pstB"
                     /locus_tag="PKL11_08805"
     CDS             complement(3..851)
                     /gene="pstB"
                     /locus_tag="PKL11_08805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNZ13740.1"
                     /translation="MLSSTSIALPVFQTRSIEGVNIDHAPAIEARDLNVYYGHFQAVK
                     GINLKIEQRQVTAIIGPSGCGKSTVLRTFNRMNELLPEARTTGEVLFMGQNIYAPDVS
                     PVEVRRRIGMVFQKANPFPRSIYENVAWGARLNGFKTGLGTPSMDEWVEHCLRKAALW
                     DEVKDKLKASGLSLSGGQQQRLCIARTIAIQPEIILMDEPASALDPIATLKIEELMRE
                     LVKEYTIIVVTHNMQQAARVSDYTAFLTVTPERSGVLVEYGPTRQIFMNPRDRRTEDY
                     ITGRYG"
     gene            complement(863..>1519)
                     /gene="pstA"
                     /locus_tag="PKL11_08810"
     CDS             complement(863..>1519)
                     /gene="pstA"
                     /locus_tag="PKL11_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNZ13741.1"
                     /translation="PVGVGAAIYLEEYAADNALNRVLKTNITNLAGVPSIIYGMLGLA
                     IFVRALQSITSGALFGAADPAGATGRTVLSAGLTLALLVLPLIIINAQEAIRTVPDSL
                     RQASLGLGATKWQTIWHHVLPNAISGILTGAILAISRAIGETAPLVVVGAATFLNFDP
                     NGPFSRFSTLPIQIYQWTSRAQGEFRNLAAAAILLLLVMLLSLNASAILLRNRFRSKI
                     "
     assembly_gap    1520..1619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1807..2763)
                     /gene="pstC"
                     /locus_tag="PKL11_08815"
     CDS             complement(1807..2763)
                     /gene="pstC"
                     /locus_tag="PKL11_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNZ13742.1"
                     /translation="MVKFQSTASEVRRTSEAISLKKRARPGEALVQAFLFFCGAFSIL
                     TTVGIVYELGKESLLFFTSPEVNLWQFMTGTVWQPQAGKFGILPLVSATLMTSGFAML
                     IAVPLGLASAIYLSEYATPRFRNTIKPILEILAGIPTVVYGYFALTFMTPLLRMIFGN
                     EVVGIYNTASAGIVMGILILPLISSMSEDALSAVPRSLREAAYGLGATKFEVAIKVVL
                     PAAVSGIAAAFLIGISRAIGETMIVAIAAGAGPAFTLNPFQAAETMTGHIVRISGGDL
                     SYNSIDYNSLFAIGLLLFVMTLAINLISQQVVRRFRKSYGSS"
     gene            complement(2768..3811)
                     /locus_tag="PKL11_08820"
     CDS             complement(2768..3811)
                     /locus_tag="PKL11_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ13743.1"
                     /translation="MKKKLSIVLAVAIALVLTLSACTPTPAPATATEVAQPTAAPVEP
                     TTQPAVQATQSPLDPAAVSGNVVTAGSSTVYPLAERMADRFTEEGFTGQITIDSIGSG
                     AGFERFCTTGETDVANASRAIKESEIEACAAIGRTPIEFRVGTDALAIVVSSENTFLT
                     DISLEDLARVYSGEAVKWSDINPAYPAEDIQRFSPGTDSGTFDYFVEGVLRPVYGKDN
                     DADKNAILNAPNIQFSEDDNVLVQGVEGSPYAIGYFGYAYFAENQAGLKAISLNGIAP
                     SLQTAEDGSYPLARPLFLYSDAKILQEKPQVAAYINFFLTNVNDEIQDVGYFPASEEA
                     LNASRQALADALK"
     assembly_gap    3909..4008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4009..5387)
                     /locus_tag="PKL11_08825"
     CDS             complement(<4009..5387)
                     /locus_tag="PKL11_08825"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNZ13744.1"
                     /translation="MFRKIRWRIALPYILLILLLMLGLQIYLSNTIEKTYLADLESQL
                     TVDARAAAEIVQPVWTDSTRLNPLIDHWAAVLDARLTLIDIYGNVIGDSAEAPQEMDN
                     HSTRPEVISALTDGQGSSIRFSHTLGVDMLYTAVRLGDPSAPLGVVRLARPISQIQAR
                     LDQIRQTLWTAMLAVALVAGLLAVWIASITSRPVERLTRAANQIAAGDFSMPTLNTSV
                     TELELLIKSFNQMALQIRSQVGDLEAERGKLAAVLDHMTDGILIVDSAGSIQLLNPAA
                     EKMFGISLPQTTGRSLPEVLRVHQPYDLWQQSAATQVTQQISFEFKKQRYLQAFAIPL
                     GSSLPGHTLLLFQDITAQKQVEVTRRDFISNVSHELRTPLAALKALTETLRDGALEDP
                     PAAHRFLERMETEVDALSLMVTELLELSRIESGRVPLNLQPVRPLDMMTAAYERLCLQ
                     AERAELSLS"
     gene            complement(5390..>5623)
                     /locus_tag="PKL11_08830"
     CDS             complement(5390..>5623)
                     /locus_tag="PKL11_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ13745.1"
                     /translation="PLALKPKEYELLLFMAQHPGQALTRARLLEQVWGWDFAGGTRTV
                     DVHVRWLREKIEADPENPVRIITVRGAGYRFEG"
BASE COUNT         1247 a         1471 c         1623 g         1082 t
ORIGIN      
        1 aattacccgt accgcccggt gatgtaatct tcagtgcgcc gatcacgcgg gttcataaag
       61 atttgccggg tggggccata ttccaccaat acccccgagc gctctggtgt gacggtcagg
      121 aaggctgtgt aatctgagac gcgagccgcc tgctgcatgt tatgagtaac aacgatgatg
      181 gtgtactctt tgaccagttc gcgcatcagt tcttcaattt tcagggtggc aatcgggtcc
      241 agggccgagg caggttcatc catcaagatg atttcgggtt ggatggcaat ggtgcgggcg
      301 atgcacaggc gctgctgctg cccgcccgag agggacaacc cgctggcctt cagcttgtct
      361 ttcacctcgt cccacagggc ggctttgcgc aggcagtgct ccacccattc atccatgctg
      421 ggcgtgccca ggccggtctt aaagccgttg aggcgggctc cccaggctac attctcgtag
      481 atgctgcgcg ggaaggggtt ggccttttgg aaaaccatgc caatgcgccg gcgaacttcc
      541 accgggctga catcgggcgc ataaatattc tgccccatga acaagacctc gccggtggtg
      601 cgggcttcag gcagcagttc gttcatccga ttgaacgtcc gcagaaccgt acttttaccg
      661 catcctgaag ggccaatgat ggccgttacc tggcgctgtt caattttcaa attgattcct
      721 ttaacggcct gaaaatgtcc gtaatagacg ttcaggtcgc gggcttcaat tgccggagcg
      781 tggtcaatat tcaccccctc aatagaccgg gtttgaaaaa cgggcagcgc gatagaagtg
      841 gaagagagca tacaacaatc tcctagattt tagagcggaa gcggttccgc agcaggatgg
      901 ctgaggcgtt gagtgaaagc agcatgacca gtaaaagcag gatagccgct gccgcaagat
      961 tgcgaaattc accctgggcg cgcgaggtcc actgataaat ttggatgggc agagtggaga
     1021 accttgagaa cggcccattg ggatcaaagt tgagaaacgt tgcggcgccc accaccacaa
     1081 gcggagcggt ttcgccaatc gcccgcgaga ttgccaggat ggcgcctgtc agaatgcccg
     1141 agatggcgtt aggcaaaaca tggtgccaga tggtttgcca tttggtggcg cccaggccca
     1201 ggctggcctg acgcaaggaa tcgggcacgg ttcggatggc ttcttgagcg ttgataatga
     1261 tcagaggcag caccagcagc gccagggtga gcccggcaga cagtacagtg cgcccggtgg
     1321 cgccagcggg gtccgccgcg ccgaacagcg caccgctggt aatgctttgc agggcgcgga
     1381 caaagattgc cagacccagc atgccgtaga tgattgaagg caccccggca aggttggtga
     1441 tgttggtttt cagaacccgg ttgagggcgt tgtcggcagc atattcttcc agataaatgg
     1501 ctgcccccac gcccaccggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1621 gcgattccct ggaaacctta tcggtcatgg cggcgtagcc catcaaacca tccagaatgg
     1681 tgaacatgag cagcgccagc agtgcaatgc ccagcagggt ggagaactga aaaacccagc
     1741 gcgccagcct gccagaccgg tagcgagagg gaaagttcac ttgaaaaacc ggagttagat
     1801 gagcgttcat gatgaaccgt atgattttcg gaaccggcgg acgacctgct ggctgatcag
     1861 gttaatcgcc agcgtcatga cgaataagag caggccaatc gcaaacaggc tgttgtaatc
     1921 aatcgagttg tagctgaggt cgccgccaga aatccggaca atatgccccg tcatggtttc
     1981 ggcagcctgg aaagggttca gcgtaaaagc ggggccagcg ccagcagcaa ttgccacaat
     2041 catggtctcg ccaatggcgc gggaaatgcc aatgagaaaa gccgccgcaa tgccagacac
     2101 ggctgccggc aagaccactt tgatggcaac ttcaaatttg gtggccccaa gaccgtaagc
     2161 cgcctcgcgg agggagcgcg gcacagctga aagagcatcc tcgctcatgg agctgatcaa
     2221 tggcagaatc agaatcccca ttacaatccc ggcagaagcc gtattataaa ttccaaccac
     2281 ctcattgcca aaaatcatcc gcagcagggg cgtcatgaac gtcagcgcaa aataaccata
     2341 caccactgtc ggaatgccag ccaggatttc cagaatgggt ttgatggtat tacggaagcg
     2401 gggtgtggca tattcgctga gataaatggc cgatgccagg cccagcggca cggcaatcaa
     2461 catggcaaag ccgctggtca tcagcgttgc actgaccagc ggcagaatgc caaacttacc
     2521 cgcctgtggc tgccatacgg tgcccgtcat aaactgccag aggttgacct ccgggctggt
     2581 aaaaaagagc aagctctctt tgcccagttc gtacacaatc cccaccgtgg tcaggatgga
     2641 aaaggcgccg caaaagaaca agaaggcctg caccagcgct tcgccgggtc gggcacgttt
     2701 tttgagggag atggcttccg aagtccggcg gacttcggaa gccgtgcttt gaaacttgac
     2761 catgggccta tttgagggca tctgccaggg cctggcggga agcgttcagc gcttcttcgc
     2821 tagcggggaa gtaacccaca tcctgaattt cgtcattgac gttggtcagg aagaagttga
     2881 tgtaagcggc aacctggggt ttttcctgca ggattttggc atcggagtag aggaagagcg
     2941 ggcgagccag ggggtaagaa ccgtcttcgg ccgtttgcag cgagggggca attccgttca
     3001 aagagatggc cttcagcccg gcctgatttt cagcaaagta agcataaccg aagtagccga
     3061 tggcgtaggg gctgccttca acgccctgaa ccagcacatt gtcatcttcg ctgaactgga
     3121 tattgggggc attgaggatg gcgtttttgt cagcatcatt gtctttgcca tagaccgggc
     3181 gcagcacacc ttcaacaaag taatcaaagg tgccagagtc agtcccgggg ctgaagcgct
     3241 gaatatcctc ggccgggtaa gccgggttga tatcggacca tttgacggcc tcgccagagt
     3301 aaacccgggc caggtcttcc agggagatgt cggtgaggaa ggtgttttcg ctgctaacaa
     3361 caattgcaag cgcgtcggtg ccaacgcgga attcaatcgg cgtgcggcca atggcagcgc
     3421 aggcttcaat ttcagattct ttgatggcgc ggctggcgtt agccacatcc gtttcgccgg
     3481 tggtgcagaa gcgttcaaaa ccggcgccag aaccaatcga gtcaatggtg atctgaccgg
     3541 taaagccttc ttcggtaaag cgatcggcca tgcgttcggc cagtggatag acggtggagc
     3601 tgcccgccgt cacaacattg ccggagaccg cggccgggtc aagcggcgac tgggtggcct
     3661 gaactgctgg ctgggttgtc ggctcaaccg gagcagcggt cggctgggcc acctctgtcg
     3721 cggtggcggg cgcaggggtc ggggtacagg cgctcagcgt cagcaccagt gcaatggcaa
     3781 ccgcaagcac gatggacaat tttttcttca ttcttctaat cctccagtat caatttttga
     3841 actgccccaa ggataccaaa ggaggtttaa cgggctggta aggaattgtt aacgtctggt
     3901 tatgaaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat agacagggat
     4021 agttcggcgc gctcggcttg caggcacagc cgctcgtaag ccgcggtcat catatccaat
     4081 gggcgaaccg gctgcaaatt caacggcacc cgcccggact cgatgcggga aagctctaaa
     4141 agctcggtca ccatcaaact gagggcatcc acctcggttt ccatccgttc caggaagcga
     4201 tgcgccgccg gggggtcttc cagcgcgcca tcccgcaggg tttcggtcag cgccttcaac
     4261 gcggccaatg gggtgcgcaa ttcatgggag acattgctga taaagtcacg gcgggtgacc
     4321 tccacctgct tttgagccgt gatatcttga aacaataaca gcgtgtgtcc gggtagtgag
     4381 ctgcccagag gaatggcaaa agcctggaga tagcgctgct tcttaaattc aaagctgatc
     4441 tgttgggtga cctgcgttgc ggcgctttgc tgccagagat cataaggctg gtgcactcgc
     4501 agcacctcgg gcagagaacg cccggtcgtt tgcggcagcg aaatgccaaa cattttctcc
     4561 gcagcaggat tgagcagttg gatgctgcct gccgaatcaa caatcaaaat cccgtcggtc
     4621 atatgatcca gcaccgcggc cagcttgcca cgctccgctt ccaggtcacc cacctggctg
     4681 cgaatttgaa gcgccatctg attaaatgat ttaatcaaca gctccagctc ggtgaccgag
     4741 gtattgaggg tgggcataga aaagtcaccg gcggcaatct ggttggcggc ccgggtcagg
     4801 cgctccaccg ggcgcgaggt tatggaggcg atccagacag ccagcagccc tgccaccaaa
     4861 gcgacggcga gcatcgccgt ccacagggtt tgccgaatct ggtcaagccg cgcttgaatc
     4921 tggctgatgg ggcgggcgag ccgaaccacc cccagcggcg cagacggatc gcccaatcga
     4981 acggcggtgt agagcatatc cacgccaagc gtatggctga agcggatact gctgccctgc
     5041 ccatctgtca gagccgagat gacttccggg cgggtgctgt gattatccat ctcctgaggg
     5101 gcttcggcag aatcgccgat gacattgcca tagatgtcga tcaacgtcag gcgggcatct
     5161 aacacagcgg cccagtgatc aatcagggga ttcagtctgg ttgagtctgt ccagacaggt
     5221 tggacaattt cagcagcggc ccgggcatct accgtaagct gcgattccag gtcagccagg
     5281 taggtttttt caatggtgtt ggagagataa atttgcagcc caagcatcaa aagcaaaatg
     5341 agcaggatgt agggcagggc aatgcgccaa cgaattttgc ggaacatatt tacccctcaa
     5401 accgatagcc agccccgcgg accgtgataa tgcgaaccgg gttttcgggg tcggcctcaa
     5461 ttttctcacg cagccagcga acatgcacat ccaccgtgcg ggtgccgccg gcaaaatccc
     5521 agccccaaac ctgctccagc aggcgcgccc gggtcagggc ctggcccgga tgttgggcca
     5581 tgaaaagcag cagctcgtat tctttgggct tgagcgccag cgg
//
LOCUS       DAOPVS010000121         5581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14498_length_5581_cov_36.576547, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000121 DAOPVS010000000
VERSION     DAOPVS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14498_length_5581_cov_36.576547"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKL11_08835"
     CDS             complement(<1..361)
                     /locus_tag="PKL11_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNZ13746.1"
                     /translation="MKLAPKARYYTLGPEVARAIQQGLPIVALESTVITHGLPRPQNL
                     ELARQMETEVRAQAAIPATIALLDQKVHVGLTETELERLANEPAARKVSRRDFGATLA
                     QGAVGGTTVAGTLIAAHM"
     gene            complement(358..1929)
                     /locus_tag="PKL11_08840"
     CDS             complement(358..1929)
                     /locus_tag="PKL11_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13747.1"
                     /translation="MTTQPTPRPGPFARMSETFRPIFQQIAIWTRPLTNLIDDVVNRL
                     FRMRYVGGLIRLALVIGFGLVGWLLLAINIHPYAHWRHLLPTLLPGMNPYVFVLSFFW
                     TILRNTLLAWDVLTYVILGLLPFFAAIEFAAVYLDDIYELNDIQTARRFIWQAAFSVP
                     DYDILHIENGEVRPEDRRLPVFRIGGPGYVQVGLENASVFERMDGFVNIIGPTTQGIL
                     RTERLEGFERLRGVIDLRDQTATPDVVGRTRDGIPVTVRNIRMLYSVIRPGDQTPTLN
                     RPYPFTNASIRTLVYDQNRDTITGAMNSLVMVAFRTFLGTRLLSEVLAAITANDVDRL
                     RGLTTSDGLIGATVWAPNRVPRPDVAERNFYDPNETNGFFAPFIERAARDGVQLNWID
                     VGTWHIPNTILSAQYLDALQVTMQNQGRNNLYALRDTTFSSWADQIQLMLRQMPLDLV
                     QLQQVRRLRPSELTEDDMVYQLVKQIQKQIRNGRDQFREIGREEPDEIQQALRYLQQY
                     EVDYARARGGRTIGA"
     gene            complement(1926..3275)
                     /locus_tag="PKL11_08845"
     CDS             complement(1926..3275)
                     /locus_tag="PKL11_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13748.1"
                     /translation="MQWLRNFIQNRLPNNPAWRRALMVIGGVLAVYLIILMAHGGQNT
                     IRAFIVEYQQFDYCFWGFIFWIFFFAQFVLPLRTVGERVYAFGRLLIYNLGLHGPAIL
                     IENGRIQERRYETERKGPGVIVLDMASAAVLRTPVEMTRTVGPGVTFTKHDFLTNISE
                     TVAEPVDLHSQRRFIGPQGNSNPFLPQQPNESPAEFEARQRMRLETRAFTRDGVEIVP
                     NIFSRFDLIHEPEEGNTPYGFNPDSVYRLARVHSINASAHPESPERMTDIYGLPPMLA
                     ANVWRELLEQYTLLEIFPFSGEPPFPLDRIRDRIKERLTTPGSREHTLMLESGLKLRF
                     AFILNLRLPPEAETDLVRRWENSWLTRARQEERLINQLHSEAAQNGRTLAEREYATAL
                     AEALKNLIAAGPPSATAGKIILAESVRGIAQLYRQHPQLHGDPETLRDLEQLLEWAK"
     gene            3416..4375
                     /locus_tag="PKL11_08850"
     CDS             3416..4375
                     /locus_tag="PKL11_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNZ13749.1"
                     /translation="MFRALFISLSKAMWAQRLITNLPFAWRAASRFVAGETPTAAIRA
                     VKELNDKGILATLDHLGENTTLPAEAVQAADEILIIIDEINRSGVRSNVSIKLSQIGL
                     NLDEELCRQNLRRILERAKATGNYIRIDMEDSPVTDKTLGLLTWAREQGFTDVGTVIQ
                     SYLFRSEADIRSLLAQGVRVRLVKGAYREPASVAFPKKSDVDANYDHLTDVLLDGALA
                     AASPVISADGRTPPILAVASHDEARIAYARTAARQRGIPESAIEFQMLYGIRRDLQES
                     LAQAGYPVRVYVPYGTHWYPYFMRRLAERPANFWFFLSNLFKK"
     gene            4372..5103
                     /locus_tag="PKL11_08855"
     CDS             4372..5103
                     /locus_tag="PKL11_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ13750.1"
                     /translation="MSASSKPLAIITGGAVRLGQVIALSLANAGYTIGLHYHTSEAAA
                     HQTARQLSAQDALAFLHSADLRDPAQVDALFARSAETGLPLRVLINSASVMPRADILQ
                     MPVSEWDDTLALNLRAPWLCARCAAKQMDAAGGVIVNLTDAGAGRVWTNYPAYIISKS
                     GLDTLTRLLARALAPRIRVNAVAPGLILPSADMPSSEWDRLVDRTPLKKAGDPQAIAD
                     AVHFLINNSHITGQTLVVDGGYQLI"
     gene            5111..>5581
                     /locus_tag="PKL11_08860"
     CDS             5111..>5581
                     /locus_tag="PKL11_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13751.1"
                     /translation="MDSKSFNPFSLILKASRPWLLLSAALFYILGAGVAIHQGQNIVW
                     SNFFFGLALIILLLLGGNYLKRFYDPPSSDPEKTPSSLSQQQAEHQIYLLAAITLLTI
                     GAGLTIVLLLQDVLTWIALLVLGIAIALVLAQGIPPFRLVYSGYGELAETAVMTL"
BASE COUNT         1218 a         1594 c         1411 g         1358 t
ORIGIN      
        1 ccatgtgggc cgcaatgagc gttcctgcta cggtggtgcc gccaactgct ccctgcgcca
       61 gcgtcgcgcc aaaatcccgc cggctcactt tgcgcgccgc aggttcattg gccagccgtt
      121 ccagttcggt ctcggtcaac cctacgtgaa ctttttggtc gagcagtgca attgtcgctg
      181 gaatggctgc ctgtgcccgc acctcggttt ccatctgacg cgccagttcc aaattctgtg
      241 ggcgcggcag cccatgtgta ataacggttg actcgagcgc tacaatcggc agtccctgct
      301 gaatggcgcg ggcaacttct ggccccagtg tataatagcg ggctttgggt gctaatttca
      361 tgctcctatc gtccttcctc cccgggcgcg ggcgtaatcc acttcatact gttgcagata
      421 gcgcagcgcc tgctggattt catcaggttc ctcgcggcca atttcccgga attgatcccg
      481 cccattgcgg atttgctttt gaatttgttt aaccagttgg taaaccatat cgtcttcggt
      541 taactcactc gggcgcagtc gccggacttg ctggagttgc accaaatcca atggcatttg
      601 acgcagcatc aattgaatct ggtcagccca ggagctgaat gtcgtatctc gcagtgcata
      661 taaattattt cgcccctggt tctgcatagt cacctgaagc gcatcaaggt actgcgccga
      721 taagatcgtg ttgggaatat gccaggtgcc cacatcaatc cagtttaact gcacaccgtc
      781 tcgtgccgca cgctcaataa acggcgcaaa aaagccattg gtctcattgg ggtcgtagaa
      841 attccgctca gccacatcgg gccgcggcac acggttgggc gcccagacgg tcgctccaat
      901 cagcccgtcg ctggtggtca agccgcgcag ccgatccaca tcgtttgccg tgatggcggc
      961 cagcacctca ctgagcagcc gggtgcccag gaaggtgcgg aacgcaacca tcaccagtga
     1021 gttcatggct ccagtaatcg tgtcgcgatt ttggtcatag accagtgttc gtattgacgc
     1081 gtttgtaaag ggatacgggc ggttaagtgt tggggtctga tcgcccggtc tgatcacgct
     1141 gtacagcatc cggatatttc ggaccgtcac cgggatccca tctcgggtgc gtccaacgac
     1201 atccggagtt gctgtctggt cgcgcaggtc aatcaccccg cgtaaacgtt caaaaccttc
     1261 caggcgttcg gttcttagaa tgccctgtgt ggttggccca ataatattaa caaacccatc
     1321 catgcgttca aacacgctgg cgttttcaag cccaacctga acataccctg gcccgccaat
     1381 gcggaacaca ggcagccggc ggtcttcggg ccgcacctct ccgttttcaa tgtgtagaat
     1441 atcataatct ggaacggaga aggccgcctg ccagataaat cgtcgggcag tttggatatc
     1501 attaagttcg taaatatcat ccaggtaaac agccgcaaac tcaattgctg caaaaaatgg
     1561 cagcagacct aaaatgacat aggttagcac atcccaggca agcagtgtgt ttcgtaaaat
     1621 cgtccagaag aagcttagaa cgaaaacata aggattcatt cctggaagca aagtaggcag
     1681 caggtgccgc cagtgagcgt atggatggat attaatcgcc agcagcagcc aacccaccag
     1741 accaaaacca ataactaatg ctaagcggat cagtccgccc acataacgca ttcggaacag
     1801 acgattaaca acgtcatcaa ttaaattggt cagcgggcgt gtccagatgg ctatctgctg
     1861 gaagatcggc ctgaaagttt cgctcatacg ggcaaatggc ccggggcggg gtgttggctg
     1921 tgtggtcatt tggcccactc cagcagttgc tccaggtcgc gcagggtttc agggtcaccg
     1981 tgcagttgtg gatgttgccg gtagagctgc gctattcctc gaaccgactc agccagaata
     2041 atctttcctg ccgtggctga aggcggacca gccgcaatca ggtttttcaa cgcttccgcc
     2101 agcgctgtcg catattcccg ttctgccagc gtgcgcccgt tttgtgctgc ctcgctgtga
     2161 agttggttga tcaggcgctc ttcctgtctc gcccgggtga gccaagaatt ttcccaacgc
     2221 ctcaccagat ctgtctcagc ctctggcggc aatcgtaaat tcaagatgaa tgcaaatcgt
     2281 agcttgaggc cgctttccaa catgagcgta tgttctcttg atcctggcgt tgtcaaacgc
     2341 tctttgatcc gatctcgaat gcggtcaagt gggaaaggtg gctccccgga gaacggaaag
     2401 atttcgagca atgtatactg ttctaatagt tctcgccaga catttgctgc cagcatgggg
     2461 ggcaagccat aaatatccgt catccgctcg ggcgattccg ggtgggcgct ggcgtttatg
     2521 ctatgcactc tcgccaatcg ataaaccgaa tctggattga acccgtatgg tgtattacct
     2581 tcttctggtt cgtgaatcag atcgaacctt gagaagatat taggaacgat ctcaacccca
     2641 tcgcgcgtaa acgcccgggt ttccagacgc atccgttgcc tggcttcaaa ttctgccggg
     2701 ctttcattgg gctgctgtgg caaaaacggg ttgctattgc cctgaggtcc gataaaccgc
     2761 cgttgactgt gcaggtcaac gggttcggcc actgtctcag agatattggt taaaaaatcg
     2821 tgttttgtaa aagtgacccc cggaccgacg gtgcgggtca tttcaaccgg cgttcttaat
     2881 acagccgcgc tcgccatgtc cagcacaatt acgcctggcc ctttgcgctc ggtttcgtac
     2941 cgccgctctt gaatccgccc attctcaatc aggatggccg ggccatgtaa acccaggttg
     3001 taaatcagca gccgtccaaa agcataaact cgctctccca ccgttctcaa tggcagcacg
     3061 aactgcgcaa agaagaaaat ccagaaaata aatccccaga agcagtaatc aaactgctga
     3121 tattccacaa taaaggcgcg gatggtattt tgcccgccgt gggccattaa gatgatcaaa
     3181 tagacggcta aaacgccccc aatcaccatc agcgctctac gccaggccgg gttatttggc
     3241 agccgattct gaataaaatt ccttaaccac tgcatagctt aattctatcc taattcagaa
     3301 aaatcaacac caaatttctc ggtcattttt taaccctcta ctgtttatat cggtgtaggc
     3361 tgttgatatg ctacaatact tgaacgaaca agatattgtg attgggagaa gcaccatgtt
     3421 cagagcactt ttcatctcat tatcaaaggc catgtgggcg cagcggctca tcacaaatct
     3481 gccgtttgcc tggcgggctg cttcgcgctt tgtcgccggt gagactccta ccgcggccat
     3541 tcgcgcggtc aaagagctga atgataaagg tattctggct accctcgatc atctgggcga
     3601 aaacaccaca ctgcccgccg aagcggttca ggcagcagac gaaattctga ttatcattga
     3661 tgaaatcaat cgctctggtg tccgttcaaa tgtttccatc aagcttagcc agattggcct
     3721 gaacttagac gaagagcttt gccgccaaaa cctgcgccga attttggaac gtgccaaggc
     3781 aactggtaat tacattcgca tagatatgga agactcgccg gttaccgata aaaccctggg
     3841 ccttttaacc tgggcgcgtg aacagggttt tactgacgta ggaacggtca ttcaatctta
     3901 ccttttccgc agcgaggcag atattcgctc gcttcttgcc cagggcgtac gcgtccggct
     3961 ggtcaaaggc gcctatcgtg aacctgccag cgttgccttc cccaaaaaat cagacgtgga
     4021 cgcaaattat gaccacctta cggacgtgct gttggacggt gccctggctg cagcctcgcc
     4081 agtcatcagc gccgatggtc gcacgcctcc cattctggca gtagccagtc acgatgaggc
     4141 tcgcatcgcc tatgcccgga ccgcggctcg ccagcgtggc attcccgaat ccgccatcga
     4201 atttcagatg ctttacggca ttcggcgtga tttgcaggaa agccttgccc aggctggcta
     4261 ccctgtccgt gtgtatgttc cctatggcac ccactggtac ccatacttta tgcgccgcct
     4321 ggcagagcgc ccggcaaact tctggttctt cctctccaac ctgttcaaaa aatgagtgcc
     4381 tcctctaaac cgcttgccat catcaccggg ggcgctgttc gccttggtca ggtcattgcc
     4441 ttgtccctgg caaatgctgg ttacaccatt ggcctgcatt atcacacctc cgaagcggct
     4501 gcccaccaga ctgcccggca gctttctgct caagatgcac tggcctttct tcactcagcc
     4561 gacttgcgcg accccgcgca ggttgatgcg ttgtttgccc gctctgccga gacgggtctc
     4621 ccgctccgcg ttctgatcaa ctcggcctca gtcatgccgc gcgccgatat cttgcaaatg
     4681 ccagtttccg aatgggatga taccctggcc ttgaatctac gcgcaccctg gctttgtgcc
     4741 cgctgcgccg caaaacaaat ggatgccgcc ggcggcgtga ttgttaacct gaccgatgcc
     4801 ggcgcagggc gggtttggac gaactacccg gcctacatta tcagtaaatc cggtctggat
     4861 actctcacgc gccttctggc gcgtgccctg gcgccgagaa tccgcgtcaa tgctgtagcg
     4921 cccggcctga ttttgccttc tgctgatatg ccatcctcag agtgggatcg tttggtggac
     4981 cgcacacccc tgaaaaaggc cggtgaccct caagccattg ctgacgctgt ccactttctc
     5041 atcaataatt cccacatcac cgggcagacc ctggttgtgg atggcggcta tcaattgata
     5101 taggagacgc atggactcca aatcattcaa tccattttcc ctgattctga aagcctcgcg
     5161 cccctggttg ctgctttctg cggcgttatt ttatatcctg ggcgcgggcg tggccatcca
     5221 tcaggggcag aacattgtct ggtcaaattt cttctttggt ttggccctta tcattctcct
     5281 gctgctgggc gggaactatc tcaaacgctt ctatgatcct ccatcatctg atcccgaaaa
     5341 aacaccatcc agcttgagcc agcagcaggc cgagcaccag atttacctgt tagctgcgat
     5401 cacgctcttg accattggcg cgggattaac aatcgtcctc cttcttcaag atgtattgac
     5461 atggatagcg ctcctggtct tagggattgc cattgcgctt gtgctggcac agggtatccc
     5521 accttttaga ctggtttatt ccggttacgg cgaactggct gagacggcag tcatgacgct
     5581 g
//
LOCUS       DAOPVS010000122         5484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14855_length_5484_cov_30.370971, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000122 DAOPVS010000000
VERSION     DAOPVS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14855_length_5484_cov_30.370971"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKL11_08865"
     CDS             <1..242
                     /locus_tag="PKL11_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13752.1"
                     /translation="MRTVPEKYSATFVSFFQSAQYISSIVAPLLGTFLAEAIGIGPAL
                     IISGIIRLLGFALFLFDRSGKKKLPVSPEAEGAVV"
     gene            239..1411
                     /locus_tag="PKL11_08870"
     CDS             239..1411
                     /locus_tag="PKL11_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ13753.1"
                     /translation="MSTDAGKTIFCTQCGQSLPVGAMICPKCGSVVSQPTTPQVESID
                     PASVKVAAQEPPKPALCQKCGRELEPGWAACPSCGDLVSPEEIQTKPGTVTPPPTDSK
                     PKRNGMGWLLGLGILALVLGCGLIVALGFLFNTTQNLLMVEGELDQVAQTATAAQAGL
                     QITLESQLATQNAAEAQLLDQQSALENQALAFSATSTAQVAADQEALRLQQEAFQATL
                     SAVMTEAGQVIYGPADGELLHKVDGFVETTWADVNVANLIIEARFYNPYGTEEGPWDY
                     GFMFRHTDTNEQYRLVISSDGQWHFIIARGDDRPELAVGPIANLDLTAGGSNLVNLWV
                     KDGTANLYVNNQWITALDVSEKIVAGDAAVCTGFYTGYEIEGKITRYEGFKVSELP"
     gene            complement(1378..2547)
                     /locus_tag="PKL11_08875"
     CDS             complement(1378..2547)
                     /locus_tag="PKL11_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ13754.1"
                     /translation="MTIIRTLVLSGGGGRGAFHAGVYKYLCESSKPGVDDEHQGSWTP
                     DIIVGTSIGAVNGAAITQGYSGEELVELWRGLREQDIQGLPPTMSWLSRSVANRVLRG
                     MIGVSLPRVPKDISTSPPPEISPALLPGMGRFSRWLLGRWGSLLDTGPLRRTLVERMK
                     INEQSLAQSQRTLLINATNVSTGQRAVFSNRPIYKRGTAQPRSDVTPGITIQRILASC
                     SIPLVYPWTVDDETRAIYWDGALVSNTPMGAALDAAANRPDEDVMEVVVVMMTPWRTQ
                     EDLGTAKAAIMPKDFGEAITYALDWVLLASFRERIDLIEAFNRLGKIGRELNDPLLSK
                     YREVRVTIVAPEDFFPAARILDYDEHNKSLIASGYQAAEKAFCRAYGSSLTLNPS"
     gene            complement(2573..3295)
                     /locus_tag="PKL11_08880"
     CDS             complement(2573..3295)
                     /locus_tag="PKL11_08880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ13755.1"
                     /translation="MQFYFIRHAQSSNNALYDSTGSWDGRSADPELTEMGWQQARALA
                     DYLQRSPADPQAAGRDAANHGGFGLTHLYCSPMLRAVDTGSVVAAALDLPLTVWPDWH
                     EGGGIYLNDSETDEPYGLPGATRAQLQARYPRIVLPDSITETGWWNRSMEAKEERPVR
                     ARRVIDDLLARHGGTEDRVAVISHGGFYNHIIWAAARAERPEKIWMVLNNTAITRIDF
                     NEDWTDMVYLNRLDHLSPGMVT"
     gene            3388..4410
                     /locus_tag="PKL11_08885"
     CDS             3388..4410
                     /locus_tag="PKL11_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HNZ13756.1"
                     /translation="MTILPTGKLPPKLLGQLLNNAPVSHPRVRLGPGVGLDCGVIETN
                     GMVQVFKTEPITFATDQIGWYAVQVAVNDIVTTGATPRWIMLSLLLPEGKTTPALVET
                     IGEQIFTACRLHNLTVLGGHTEITTGLDRPILVATVVGETTPERLITPQGAQVGNKIL
                     LTKSAGIEATAILAREMAERLQDALLPEEIEAAQNYLYEPGISVWKDAQIAQQAGRVT
                     AMHDPTEGGIVTALWELAEASDKTLQVKPAAIPISPITEKLCNALKIDPLAAISSGAL
                     LMTAAAEDSPAICRALAEAGIACAVIGDVSEGPADVQINTRCGPQRLYQVERDEVARL
                     FETPMP"
     gene            4560..5264
                     /locus_tag="PKL11_08890"
     CDS             4560..5264
                     /locus_tag="PKL11_08890"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNZ13757.1"
                     /translation="MKRSTLIIVIGVIAALLLVTAQAFAAAPQADLTGKPTPKPHDGQ
                     IDDDKGKPDKPFKLNQGKKEVYHGVILSVDAASLTVQMEDGTTATVALTAETRLHVPT
                     LGLTATAADLLPEMRVMVKARPDEAGILTALSVNVIMGDGEGDDDMEEPEPGESAGIV
                     TEYIPGTSITIQMADGAVISYGLSPNVKIVPHRRADLLDVGASVTVVLRDDMTGAMLT
                     AKGIVVHPPAGDEDGD"
     gene            5324..5395
                     /locus_tag="PKL11_08895"
     tRNA            5324..5395
                     /locus_tag="PKL11_08895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5357..5359,aa:Arg,seq:ccg)
BASE COUNT         1268 a         1405 c         1662 g         1149 t
ORIGIN      
        1 tgatgcgcac cgtgcccgag aaatacagcg cgacgtttgt atcattcttt cagagtgcgc
       61 aatacatttc atcaattgtg gcgcccctgc ttggcacgtt tctggctgaa gccattggaa
      121 ttggaccggc attgatcatc tctggcatca tccggctgct gggttttgct ctattcctct
      181 ttgatcgatc tggaaagaag aagctcccgg tatcaccgga ggcagaagga gctgtggtat
      241 gagcacagat gctggtaaaa ctattttttg cacacagtgc ggtcaatctt tgccggtggg
      301 ggccatgatc tgccccaaat gcggatcagt ggtaagccag ccgacaacgc cccaggttga
      361 gtcaattgac ccggccagtg tgaaggtagc ggcacaggaa ccgcccaaac cggcactatg
      421 ccagaaatgc gggcgagaac tggagcccgg ctgggcagcc tgtccaagct gtggggatct
      481 tgtctcccca gaagagattc agaccaaacc tggaacagtc acgccgccgc caacagacag
      541 caaacccaag cgcaatggga tgggctggct gctgggactg gggattctgg cgctggtgtt
      601 ggggtgcggg ctgattgtgg cgctggggtt tctcttcaac accacccaga atttattgat
      661 ggtcgagggg gaactggacc aggttgctca aacggctacc gcggcgcagg ccgggctgca
      721 aattaccctg gaaagccaac tggcaaccca gaacgcggcg gaagcgcaac tgctggacca
      781 acaatcggcg ctggaaaatc aggcgctggc tttcagtgcg accagcaccg cacaggttgc
      841 cgcagaccaa gaggcacttc ggctgcagca agaggctttt caggctacgc tgagtgcagt
      901 gatgaccgag gcaggacagg tgatttatgg acctgccgat ggggaactgc tgcacaaggt
      961 ggatggattc gtcgaaacga cctgggcgga tgtcaatgtc gccaatttga tcattgaagc
     1021 gcgtttctat aatccttatg gcactgaaga gggcccctgg gattatggtt tcatgttccg
     1081 gcatacagac accaatgaac aataccggct ggttatcagc tctgatgggc agtggcattt
     1141 catcattgcc cggggagatg accgaccaga gttagcggtt gggccgattg caaatcttga
     1201 cctgacggcg ggaggatcta acctggtgaa cctgtgggtg aaagacggaa ccgcgaacct
     1261 atacgtcaac aaccagtgga ttacagcgct ggacgtttct gagaaaattg tggcaggcga
     1321 cgcggcggtg tgtactggat tttatacggg ttatgaaatt gaaggcaaaa tcacccgtta
     1381 tgaaggattc aaggtgagtg aactgccgta agcgcggcaa aaggcttttt cggcggcctg
     1441 gtagccggat gcaatcaacg atttgttgtg ctcgtcgtaa tctaaaatcc gggcagcggg
     1501 gaagaagtct tcgggagcca caatggtcac acgcacctcg cggtatttac tgaggagcgg
     1561 atcgttcagc tcgcggccaa ttttgcccaa gcggttgaag gcttcgatca ggtcaatacg
     1621 ctcgcggaaa gaggccaaca ggacccagtc caacgcataa gtgatggcct cgccaaaatc
     1681 ttttggcatg atggctgcct tggccgtgcc cagatcttcc tgcgtacgcc agggcgtcat
     1741 catcaccacg acaacttcca tcacgtcttc atccgggcgg ttggcagcag catcgagtgc
     1801 ggcgcccatg ggcgtattgg atacgagggc gccatcccaa tagatggcgc gggtttcgtc
     1861 gtccaccgtc caggggtaga ccagtgggat gctgcagctt gccagaatgc gctggatggt
     1921 gatgccaggt gtgacgtctg aacggggctg ggcagtgccg cgtttataga tgggacggtt
     1981 gctgaagaca gcccgttggc cggtgctgac attggtcgcg ttaatcaaga gtgtgcgctg
     2041 gctttgggcc aggctctgct cattaatttt cattcgttca accagggtac ggcgcagggg
     2101 gccggtatcc agcaggctgc cccagcgccc caacagccag cgtgaaaagc gtcccatgcc
     2161 tggcagcagg gccgggctaa tttctggcgg cggcgaggtg gaaatatctt tggggacgcg
     2221 tggcaacgat acaccaatca tcccgcgcag gacgcggttg gcaacgctgc ggcttaacca
     2281 gctcatagtg ggcggcaggc cctggatgtc ctgctcacgc agcccgcgcc agagctcgac
     2341 cagttcttct ccggaatagc cctgggtaat ggctgcgcca ttgaccgcgc caatggaggt
     2401 gcccacaata atatccggcg tccaacttcc ctggtgttcg tcatctacac cgggtttgga
     2461 agattcgcac agatatttgt aaacgccggc atggaaggcg ccccgaccgc ccccacccga
     2521 aagaaccaac gtcctaataa ttgtcacggg aaacctcctg aaggttaagc gatcaggtca
     2581 ccatccctgg agagaggtga tccaaacgat tgagatagac catatcggtc cagtcttcgt
     2641 tgaaatcaat gcgcgtgatg gcagtgttgt tcagaaccat ccagattttt tcggggcgct
     2701 cggcgcgggc agcagcccag atgatatgat tataaaagcc gccatggctg atgaccgcca
     2761 cacgatcttc ggtgccacca tgccgggcca acagatcatc gatcacgcgg cgggcgcgaa
     2821 ccgggcgctc ttctttggcc tccatggagc ggttccacca gccggtctca gtgatggaat
     2881 cgggcagaac aatgcgcgga tagcgggcct gtaattgggc acgggttgca ccaggcaacc
     2941 cgtagggttc atcggtttca gaatcgttga ggtaaatgcc gccaccttcg tgccaatcgg
     3001 gccaaacggt gaggggcaga tcgagcgcgg cagctaccac cgagcccgtg tccacggcgc
     3061 ggagcatggg gctgcaataa aggtgggtaa ggccaaaacc gccgtgatta gcagcatcac
     3121 ggccggcagc ttgcgggtct gccggggaac gctgtaaata atcagccagg gcccgggctt
     3181 gctgccagcc catttcggtc agttcaggat cggcactgcg gccatcccag gaacccgttg
     3241 aatcgtacag cgcgttatta gaagattggg cgtgccggat aaagtaaaac tgcattgggc
     3301 gcgtctcctt gtcaatttgt tcatcctatt ttattataag ctaagctttt tgggttattc
     3361 taaggccaca aacttcaagg aattttgatg acaattttac cgaccgggaa attaccgcca
     3421 aaactactgg gacaactgct gaacaatgcg ccagtatcgc acccgcgggt gcggttaggg
     3481 ccgggggtgg ggctggactg cggcgtgatt gaaaccaacg gcatggtaca ggttttcaag
     3541 accgaaccca ttacttttgc aaccgatcaa attggctggt atgctgttca ggtggcggtg
     3601 aatgatattg tgaccactgg agcaacgccg cgctggatta tgttgagcct gctgctgcca
     3661 gaggggaaaa caaccccggc gctggttgaa acgattgggg agcaaatttt tacggcctgc
     3721 cggctccata acctgacggt gctgggcggg catacggaga tcaccaccgg cctggaccgc
     3781 cccattctgg tggcgacggt ggtgggtgaa acaacgccgg aacggctgat tacgccgcaa
     3841 ggcgcgcagg tggggaacaa gatactgctc accaaaagcg cagggattga agccacagca
     3901 attctggcac gcgaaatggc tgaacgatta caggatgctc tcctccccga agagattgaa
     3961 gcagcccaaa attacctata cgaacccggg atcagtgttt ggaaagatgc gcagattgcc
     4021 caacaagccg ggcgcgtgac cgccatgcac gatccgacag aggggggcat tgtgacggcg
     4081 ctgtgggaac tggctgaagc cagtgacaaa accctgcagg tcaaaccggc ggcaattccc
     4141 atcagcccaa tcaccgaaaa gctgtgcaac gctctgaaga ttgatccgct ggcggcgatc
     4201 tcatcggggg cacttttaat gactgcggcg gcagaagaca gcccagcgat ctgccgggcg
     4261 ctggcagaag caggcattgc ctgtgctgtc attggagatg taagtgaagg accagcagat
     4321 gtgcaaatca acacacgctg cggtccgcag cggctctatc aagttgagcg ggatgaggtt
     4381 gcaaggctgt tcgaaacccc tatgccttaa acatcggctg acatcgccaa attaggtata
     4441 tctacggatt gatttgggga cgcagaaccg gtatggttaa tctggatttg gtgtacggat
     4501 gatgtgaacc gctgctggtg accgccagaa gcacttaatt tgatggaggt taatgcgtga
     4561 tgaaacgttc aactttgatt attgtgattg gtgtgattgc ggcgctcctt ttagttacag
     4621 cacaggcttt cgccgctgcc ccacaggctg acctgaccgg taagcccacc cccaagcccc
     4681 atgatggaca aattgatgat gacaaaggaa agccggataa gccattcaag cttaatcagg
     4741 gcaagaaaga agtttatcac ggggttattt tgagcgtgga tgctgccagc ctgaccgtcc
     4801 aaatggagga tggcacaacg gctacggtgg ctttgactgc cgaaacccgg ctgcatgtgc
     4861 ccacactggg gctgaccgcg accgcggcgg atctgctgcc ggagatgcgg gtgatggtca
     4921 aagctcgccc cgatgaagcc gggatactga cagcgctaag cgtgaatgtg attatggggg
     4981 atggtgaagg cgatgacgat atggaagagc ccgagccagg tgagagcgca gggattgtga
     5041 ctgagtatat ccccggaacc agcatcacaa ttcaaatggc tgatggtgct gtgatttcat
     5101 atgggttgag ccccaatgta aaaattgtgc cacatcgtcg ggccgatctg ttggatgtgg
     5161 gtgcttcggt cactgtggta ttgcgcgacg atatgaccgg cgcaatgctg accgccaaag
     5221 ggattgttgt tcatccgcct gccggggatg aagacggcga ttaacgtcag cagatctcca
     5281 gtttgccaag cagcacaggt cgtggtaaaa tcgatctgtt cgcgtccctg tagctcagtg
     5341 gatagagcgc tggcctccgg agccagaagc ggcagttcga gtctgcccag ggacgcctca
     5401 aagacgccga gaggcgtctt tttttatagg atttgattac cggacacaat ttttctccaa
     5461 aaggttaaag gagaccggga ttgg
//
LOCUS       DAOPVS010000123         5482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_14865_length_5482_cov_32.588539, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000123 DAOPVS010000000
VERSION     DAOPVS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14865_length_5482_cov_32.588539"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    337..436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <437..1132
                     /locus_tag="PKL11_08900"
     CDS             <437..1132
                     /locus_tag="PKL11_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ13758.1"
                     /translation="ESASTCTPPIELLSSGVRVAEHTESASTCTPPIELLSSGVRASA
                     RMIWQAETSMFSVSYHRPPHLYLDDTWYMVTAHTLGHTPILVSEEHFEVWQNTFQELT
                     AQFSLTVAAWVVLPNHYHTLMHLPVGNHLGEFVQRFHGRTSRAFNQLDERKGRKIWYT
                     YWDQCIRDESTYWTRFNYIHYNPVKHGYATGPSFWRYSSYLEYLNIRGQSWLDDCWLH
                     YPVAEALTEEIEK"
     gene            1318..2238
                     /locus_tag="PKL11_08905"
     CDS             1318..2238
                     /locus_tag="PKL11_08905"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNZ13759.1"
                     /translation="MPSISPPTSRDRALSALAHAGVLIPGFGLLAPLLVWRSERDQQR
                     ETAFQALQALGWQVLQVFFVQVTALILGLIYIFIVMVSGTAGLVDNADGLTTTGAVVL
                     VVAVIGLAVIYALIGLLGAVLCLTGRSFRYPWLGPKLADYLLVSPDITTLHPVHADRW
                     LAALGHWGIFIPIMGLFTPLMAWLTQPDASPWLKNQSIQTVIYQTIGLVLGGLLQVLV
                     SFLMIPLTMVIPLALAQPDASGDFPVFMIIIGLLVLIVFAILALLVPLYSLVGFIGGI
                     RVLQGKDFNYPLLGRWFKAPVEARSTDDPL"
     gene            2225..3667
                     /gene="gatB"
                     /locus_tag="PKL11_08910"
     CDS             2225..3667
                     /gene="gatB"
                     /locus_tag="PKL11_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNZ13760.1"
                     /translation="MTPFETVIGLETHIQLNTVTKIFCGCKADSWFDPPNTNICPVCA
                     GQPGALPVLNRAAVARAVLLAAAMHATLNPLSFFDRKNYFYPDLPKGYQITQYDCPVA
                     SGGYLDLPLPGGAVRRVNIIKLHLEEDAAKTKNVGYERYIDFNRCGVPLVELVTAPDL
                     RSADEAAQYLIRLRQLLRWLGVSEADMERAQLRCDANVSIRPAGSTELNPKTEIKNVN
                     SIEAVRAAIAHEAERQMREVEAGRRVQPWTLEWDADAGVLRKLRAKETEADYRYFREP
                     DLLPIRLTDAWRDEILSALPELPLERRARFMTQYDLPEYDADILTSERSLSDYFEQAV
                     RAYGGDPKRVSNWLMNDVLRLLNERALTAGQLRLTPAALAEIIRLVDSGAINISTGKA
                     LLEKAEASGEPPAAIVQAEGLARVSDDAAIRAVCAAVLAQNPSEVASYRSGKLTLLGW
                     FVGQVMKQMHGQADPTLARQILEELLRSRL"
     gene            3874..5310
                     /locus_tag="PKL11_08915"
     CDS             3874..5310
                     /locus_tag="PKL11_08915"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNZ13761.1"
                     /translation="MNSYLSSRIAFVAVLILTFGQLTPQPVVASAPEAASIVVSSHAD
                     VINYDDHLCTLREAIIAANTDSAPVIAIGECLAGSGSDTILLESGIYTFTIAGREENA
                     SLTGDLDITSPITIQGSGIDCLNPTKECTQINADSLDRVFDVSRHGNLTLEYVLIHNG
                     STYNESGGGIYNWGNLTLDNAAIVDCHAATGQGGGLYNDGSASISHSLIVDNQADTHG
                     GGIFNMGELVVNYSQFTTNHSGAYGGGIFVTDGITTIDHSIFNNNTSAISGGGIYIQP
                     PATISNTLFYQNSATDSGGAFFQNGYYTSTFTNVTMSGNTAENGGAAAVEMGAEANLL
                     NVTIFGNQATSQGDGIFVDGADATVNIQNTLIAGNFEANCAGTITSLGNNLEDADTCG
                     LSGTGDLVNTAPLLGVLEDNGGFSFTHALLPDSPAIDAGSETTCPDVDQRGVLRPQDG
                     DLDGTAVCDIGAFEVNHLKYIYLPVLQK"
BASE COUNT         1138 a         1589 c         1458 g         1197 t
ORIGIN      
        1 gcggcaccgc caccatcacc gtcaccgtgc ctgcaccggc gggcctcagc ggcgccgccg
       61 ataccgtcac actgcgggcc gtctcgcagg gcgacgacac cgccgccgac accctcgacc
      121 tgaccaccac ggttggctgg ctcatccacc tgccgctggt gctcaagtaa ttgcacgctt
      181 aaccaggccg ggcagccgcc cggccaatcc ctccgttttt tagagtgcag gctgcccaac
      241 acactggcag cgccctgacc tgcctgatgt agcggcggcg gcctgcaccc cgccaataga
      301 attgctttct tctggagtgc gggtcgcgga gcacacnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnngaaa gtgcttcaac ctgcaccccg ccaatagaat tgctttcttc
      481 tggagtgcgg gtcgcggagc acactgaaag tgcttcaacc tgcaccccgc caatagaatt
      541 gctttcttct ggagtgcggg cttcagcccg catgatttgg caggctgaaa cctctatgtt
      601 tagcgtatcc tatcatcgtc caccccatct atatcttgat gacacatggt atatggtcac
      661 ggcgcacaca ctagggcata cgcctatcct tgtgtccgaa gaacattttg aagtctggca
      721 aaacactttt caggaattaa ctgctcaatt ttcattgaca gtggctgcct gggtcgtgct
      781 gccaaaccat tatcacactt taatgcacct gccagtcggg aatcacttgg gtgaatttgt
      841 tcaacgtttc catggtcgca catcccgtgc cttcaaccag cttgatgagc ggaaagggcg
      901 taaaatttgg tatacctatt gggaccaatg cattcgtgac gaatcaacct attggacgcg
      961 tttcaattac attcactaca acccagtcaa gcatggctac gccaccgggc cgtctttctg
     1021 gcgatattct agttatctag agtacttgaa tattcgcggt cagagctggc tggatgattg
     1081 ttggctgcac tatcccgtag ctgaagcgtt aaccgaagaa atcgaaaagt aggactaccc
     1141 acccaggaac gaaggttgcg caacgtgaat gttaaaatcc ggcttctctt ggattgcaga
     1201 tttcaacctg catcgtcatg cgggctgaag cccgcactcc aagaaaccgg aggattccta
     1261 ttaaacccca aaatcgtcta aaataaacac atccaaagac catttccgag aaacattatg
     1321 ccctcaattt ctccccccac ctcccgagac cgtgccctct ctgccctggc ccacgccggc
     1381 gttctgatcc ccggctttgg gctgctggcg cccttgctgg tctggcgcag tgagcgtgac
     1441 cagcagcgcg agacggcttt tcaggccctg caggccctgg gctggcaggt gctgcaggtt
     1501 ttcttcgttc aggtcaccgc gctgattttg ggcctgatct atattttcat cgttatggtt
     1561 tcaggcactg ccggcttggt ggacaatgct gatgggctga cgaccaccgg cgccgtggtg
     1621 ctggtggtgg ctgtgattgg tctggcggtc atctacgcgc tgatcggcct gctcggcgca
     1681 gtgctctgcc tcacggggcg cagcttccgc tacccctggc tgggccccaa actggccgac
     1741 tacctgctcg tctcgccgga tatcaccacc ctgcaccccg ttcacgcaga ccgctggctg
     1801 gcggcgctgg ggcactgggg catcttcatc ccgatcatgg gattgttcac cccgctgatg
     1861 gcctggctga cgcagccaga cgcctcaccc tggctcaaaa atcagtctat ccagacggtc
     1921 atctaccaga ccatcggact ggtgctgggc gggcttctgc aggtcctggt cagctttttg
     1981 atgatcccgc tgacgatggt tattccgctg gcgctggcgc agccagacgc ttccggcgat
     2041 ttcccggttt tcatgatcat catcggcctg ctggtgctga ttgtttttgc catcctggcg
     2101 ctgctggtgc cgctctacag cctggtggga ttcattggcg gcattcgggt actgcagggc
     2161 aaagacttca actacccgct gctgggccgc tggttcaaag ccccggttga ggccaggagc
     2221 accgatgacc ccctttgaaa ccgtgatcgg gctggagacg cacatccagc ttaacacggt
     2281 taccaagatt ttctgcggct gcaaagccga ctcgtggttc gacccgccca acaccaacat
     2341 ctgcccggtg tgtgcggggc agccgggcgc gctgccggtg ctgaaccgcg ccgccgtggc
     2401 cagggccgtg ctgctggcgg ctgccatgca cgccaccctg aacccgctct cattctttga
     2461 ccgcaaaaac tacttttacc ctgacctgcc caagggctat cagatcaccc agtacgactg
     2521 cccggtggct tccggcggct atctggacct gcccctgccc ggcggggcgg tgcggcgcgt
     2581 caatatcatc aaactgcacc tggaagaaga tgccgccaag acgaaaaacg tgggctacga
     2641 gcgctatata gacttcaacc gctgcggcgt gcccctggtc gaactggtta ccgcacccga
     2701 tctgcgctct gccgacgagg ccgcccagta tctcatccgc ctgcgccaac tgctgcgctg
     2761 gctgggcgtc tcagaagccg acatggagcg cgcccaactg cgctgtgatg ccaatgtctc
     2821 catccgtccg gcaggttcca cggaactcaa ccccaagact gagatcaaaa acgtcaactc
     2881 gattgaggcc gtgcgtgcgg ccattgccca cgaagccgaa cgccagatgc gcgaggtgga
     2941 agccgggcgg cgggtgcagc cctggacgct ggagtgggac gcggatgccg gggtgctgcg
     3001 caagctgcgc gccaaagaga ccgaggccga ttaccgctac ttccgcgagc ccgatttgct
     3061 gcccatccgg ctgacggatg cctggcggga cgagatttta tcggccctgc cagagctgcc
     3121 cctggagcgc cgggcccgct ttatgaccca gtacgacctg cccgaatacg acgccgacat
     3181 cctcacctcc gagcgcagcc tgtcagatta ctttgagcag gcggtcaggg cctatggcgg
     3241 cgaccccaag cgcgtctcca actggctgat gaatgacgtg ctgcggctgc tcaacgagcg
     3301 cgccctcaca gccggtcaac tgcggctgac gcccgccgcg ctggctgaga tcatccggct
     3361 ggtcgattcg ggcgccatta acatctcaac tggcaaggcc ctgctggaga aagccgaagc
     3421 cagcggcgag ccccccgcgg caatcgtcca ggccgagggc ctggcgcgcg tcagcgatga
     3481 cgccgccatc cgggcggtct gtgcggcggt gctggcccaa aacccatccg aggtcgccag
     3541 ctatcgcagc ggcaagctca ccctgctggg ctggtttgtg ggccaggtga tgaagcagat
     3601 gcacggtcag gccgacccca ccctggcgcg gcagattctt gaagaactgc tgcggtcacg
     3661 gctttaacat cgtcagactg gcatgatcga taatctcaaa gcggaatagc aggtatggcg
     3721 aattgccggg tcaagacgcg ggtgacaata gcgcttctgt gcaagcctaa agcgcaagtc
     3781 acaggaccta ttctggaatg ctgtaataat tttcatgtat acttgttaca tccctgacat
     3841 taaaataatc taatcttttt tgggaggatt gccttgaata gctatctgtc atcaagaatt
     3901 gccttcgttg ctgtactgat ccttaccttt ggacagttga caccgcagcc agttgtcgct
     3961 tctgcgcctg aagccgccag catcgtcgta tcttcccatg cagatgtcat caattacgat
     4021 gaccacctgt gtacattacg cgaagcgatt atcgctgcca ataccgactc ggccccggtt
     4081 attgctattg gagaatgcct ggcagggtca ggttctgata cgatcctgct tgaatccgga
     4141 atctacacct tcaccattgc cgggcgcgag gaaaacgcat ctcttaccgg ggacctggac
     4201 atcaccagcc cgataacgat tcaaggatct ggcatcgatt gtctcaatcc tacgaaggaa
     4261 tgcacacaga tcaacgcaga tagtttagac agggtttttg atgtgtccag gcacggtaac
     4321 ctgaccctgg aatatgtcct cattcacaat gggtctacat ataatgaaag cgggggagga
     4381 atatacaact ggggtaatct gacgctggac aacgctgcaa ttgttgattg ccatgccgct
     4441 actggtcaag gaggggggct ttataatgac ggtagtgcat ccataagcca ttccctgatt
     4501 gtggacaatc aggctgatac tcatggtggg ggaatattca atatgggcga attggtggtc
     4561 aattacagcc aatttaccac caatcacagc ggcgcatatg gcggtggaat atttgtcaca
     4621 gatggtataa cgactatcga tcattcaatt ttcaacaaca atacatctgc tatttctggg
     4681 gggggaatat atatccaacc acctgcaacc attagtaaca cgctctttta ccagaattca
     4741 gccacagatt ctggcggtgc attttttcag aacggttatt acacctccac ctttacgaac
     4801 gttaccatga gtggtaacac agccgaaaat ggcggtgcag ccgctgtgga aatgggagca
     4861 gaggccaatc tgctcaatgt caccattttt ggaaatcagg caacctcgca gggagatggt
     4921 atttttgttg acggtgcaga tgccacggtg aacattcaaa acacattgat tgccgggaat
     4981 tttgaggcga actgtgccgg gacgatcact tctctgggca ataatctgga agacgcagac
     5041 acctgcggct tgagtggaac cggtgatctg gtgaacacag cccccctgct gggagtgttg
     5101 gaagataacg gtggattctc attcacccat gcgctgcttc ctgacagccc ggcaattgat
     5161 gcaggctctg agacgacctg cccggacgtt gatcagcgtg gtgttcttcg accacaagat
     5221 ggggacctgg atgggacggc cgtgtgtgac ataggcgcat ttgaggttaa tcatctcaag
     5281 tatatctacc tccctgtcct ccaaaaataa caccctgaac catgctgctc aaagcatcgc
     5341 gtcaggaatc ccggattgaa attttgcgct gctcttgagg cggtgccttg ggggtggggc
     5401 actgatgcta taattacccc cagatttccc accccgcaca ggatttgcta tggacactgc
     5461 ccctgccgaa tccacaccca tc
//
LOCUS       DAOPVS010000124         5415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15100_length_5415_cov_56.853545, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000124 DAOPVS010000000
VERSION     DAOPVS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15100_length_5415_cov_56.853545"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKL11_08920"
     CDS             <1..1066
                     /locus_tag="PKL11_08920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNZ13762.1"
                     /translation="SVVGDVEIFSILLLSLGIALVLTGPMLWLTRRVGLLDLPGREPH
                     KQHQEAIPLAGGLILIATVIIGSLIYGFAAEWQDVLFLVALVLVCGFGAWDDWKAMRP
                     RWKLVGQILAAGLMIAAGVQVTIFASQAVNLIITMIWLVGITNAFNFVDSMDGLATGL
                     GVLTLTCFMLVSNAAGQYSLTSISAVGLGGLIAVYYFNARPARLFLGDSGSQLIGLLL
                     GALAVEYNPVGYTPLTSWFTPILILAVPLFDTLLVIISRARRGVPVYQSNLDHTYHRL
                     VAIGMNTNRATLTIHMAAFLLDCLAFMAIKLPPVLANVVYSAAVLAGLIVIGYLDSAP
                     QMRRLKARWQRTTGGQTPAP"
     gene            complement(1017..>1244)
                     /locus_tag="PKL11_08925"
     CDS             complement(1017..>1244)
                     /locus_tag="PKL11_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13763.1"
                     /translation="QMRATQMPEPALYILDQSGPAIYQFSLMLNLKQQYRPGLYLEMP
                     LPNRLPTAFALTSDRVMVLAFDHQLFAAILP"
     assembly_gap    1245..1344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1345..2939)
                     /locus_tag="PKL11_08930"
     CDS             complement(<1345..2939)
                     /locus_tag="PKL11_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13764.1"
                     /translation="MFDLYFYALNLKTGQPQGSLPGLLAEAAPRGAARARTQDLLCAL
                     LTFEDGQTPPPDLIKTWLAELSNAYFTAPGSITAGLRQAVRTINETLLQYKQQTPGAG
                     QLGGVLNLAVLHGDSIVIAHAGVTHSFSLTRAPVRDDHDPDVINLGLSHDLIPRFYST
                     TIQPGDMLVFCPRPPQSWLANADGSTLSREAFRRRLLLQTSADLQALIVQFQTGKGQV
                     RAAAPRVPGTAPAAELAGTPELHPSGPVYITGDTAPLEPAEPRPTVEVKRPAAESAAP
                     AAASPRPRRQPVERPAPQWQRGLLNFIRRIQAATARLSQGLNTTLARIIPGSAERLPS
                     LSPAALLFIAIAVPVLVVAIATTVYVQNGRGAQHVAYLQQAQQFAGNAVLENDPTLQR
                     NAWQSALYWVDKAEQYGITESSQSLRRTAQDALDLMDHVQRLEFTPAMDVSFESNIQI
                     TRMVSTLSDDIYAIDAISGRVIHLKYARPVFEVDQNFICGPGQVGSLIIGQLVDLVAL
                     PAGNPHYATIMAIDGAGNLLYCSP"
     gene            complement(3043..3489)
                     /locus_tag="PKL11_08935"
     CDS             complement(3043..3489)
                     /locus_tag="PKL11_08935"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNZ13765.1"
                     /translation="MEFQTAAHQATYEKLVPWVKELFGTFAHAREDAPTFGLAVGSAY
                     VQIGVMAWGEDDATITTRAYVVTGVELTPDLMHFLLRENDRMRFGAFGVDQDNDIFFE
                     HTIVGSTCDMEELKSSVLAVVYTADQYDDQIIQKWGGQRAVDRRSS"
     gene            complement(3508..>5415)
                     /locus_tag="PKL11_08940"
     CDS             complement(3508..>5415)
                     /locus_tag="PKL11_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ13766.1"
                     /translation="AQAQQRGKMQASERDVATIILTRAGYKVAEWGSLQLHGSSSESA
                     EPSGGGEPFKPGSGASATPALDKYGRDLTQMAREGKLSPIIGRHDEIEMTVETLCRRS
                     KRNPVLVGPAGVGKTAIVEGLAQMIVTGKVPQMLKDCRIIAIQPSVLVAGASMSGELE
                     KRVQAVVREASQPGIILFIDEIHTIMGSGGMLGTSDMGSLLKPSLARGDIAVIAATTN
                     DEYRRFIENDAALERRFNPVRVHELSLDETMQVIQTLRDEFVKSRTITISDEILAWLL
                     QFGDQYMRNRQFPDKAVDLLEQCVAHAVTQGKEAVDIDDAQEVAQRMVGMPLALEDRL
                     SELEKQLTGQGLLGKAETAALINRLQVTMRGLDMRSDRPNAIIMLTRDAKENSEMLAQ
                     VIAQAVYGGEDRVIAIDFARFLHPEDVNLLVGAPPGYIGYTDSLPLHRLAQIPWCVLR
                     FENVDSCHPYIREVLTQAFRDGNLMDGRGKLMHLSDTIVLITADIEIETQRSLGFFQQ
                     TAEIGTDDIESAVRASIGEDLASQLDLVLFGAHGEEGISPEWLEQNLLNEVAQRYSKQ
                     GLQIQWDNTLIEWLMEALSQGFSDSDWERWVDNILTPALIQHLPHARAAQKETVTVKI
                     QDGEIAVSKPQ"
BASE COUNT         1046 a         1406 c         1749 g         1114 t
ORIGIN      
        1 tagcgtggtg ggcgatgtag aaattttcag cattttactc ttatccctgg gcattgcact
       61 ggtactgacc gggccaatgc tttggctgac ccggcgagtg gggctgctgg atttgcccgg
      121 gcgcgagccg cacaaacagc atcaagaggc catcccgctg gctggcggat tgattctcat
      181 tgccacggtg ataatcggca gcctgatcta tgggtttgcg gctgaatggc aggatgtgct
      241 ctttctggtg gcgctggtgc ttgtctgcgg gtttggcgcc tgggatgatt ggaaagccat
      301 gcgcccacgc tggaagctgg ttgggcagat tctggcagcg gggttgatga ttgcggcggg
      361 ggttcaggtg acgatcttcg ccagccaggc agtcaatttg atcatcacca tgatctggtt
      421 ggtggggatt accaatgcgt tcaactttgt agacagcatg gatgggctgg cgactggttt
      481 gggggtgctg acgctgacct gttttatgct ggtgagcaat gctgcggggc agtattcatt
      541 gacgtcaatc agcgcggtag ggctgggggg attgatcgcc gtctattatt tcaatgcccg
      601 cccggcacga ttgtttctgg gcgactcggg ctcgcagtta attgggctgc tgctgggcgc
      661 cctggcagtg gaatacaatc cggtgggcta caccccgctg acctcctggt tcacccccat
      721 tttgatcctg gccgtgccgt tgttcgatac actgctggtg attatctcgc gggcgcggcg
      781 cggtgtgccg gtttatcaat ccaacctgga ccatacctac caccggctgg tggcaattgg
      841 catgaatacc aaccgcgcca cactgaccat ccatatggct gcgtttttgc tggactgcct
      901 ggcttttatg gcaatcaagc tgccgcccgt gctggcgaat gttgtttaca gcgcggcggt
      961 tttggccggg ctgattgtga ttgggtatct tgactcagcg ccacaaatgc ggcggcttaa
     1021 ggcaagatgg cagcgaacaa ctggtggtca aacgccagca ccataacccg gtctgaggtt
     1081 aatgcaaagg cggtgggcag gcggttgggc agcggcattt ccaggtaaag gccggggcgg
     1141 tactgctgct tgaggttcaa catcagactg aattgataga ttgccgggcc gctctgatcc
     1201 agaatgtaca gggcgggttc gggcatctga gtggcgcgca tctgnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnggactg caatacagga gattgccagc gccgtcaatc
     1381 gccataatgg ttgcataatg gggattgccc gccggcaggg caaccagatc aaccaactga
     1441 ccaataatca gagagccgac ctggccgggg ccgcaaatga agttctggtc aacctcaaac
     1501 acagggcggg catacttgag gtggatgacc cggccgctga tggcgtcaat ggcataaata
     1561 tcatctgaaa gcgttgagac catgcgggtg atttgaatgt tgctctcgaa gctgacatcc
     1621 attgcggggg taaattcaag gcgttggaca tgatccatca aatcgagcgc atcctgggcc
     1681 gtgcggcgca gggattggga ggattcggtg atgccgtact gttcggcctt atccacccag
     1741 tagagcgcag actgccaggc attacgctgc aaggtagggt cgttttccag gacagcatta
     1801 ccggcaaact gctgggcctg ctgcaaatag gccacatgct gtgcgccgcg gccattttgc
     1861 acatagacgg tggtggcaat tgccacgacc agcaccggta cagcgatggc aataaaaagc
     1921 agggccgccg gtgaaagcga cggcaggcgc tcggcgctgc ccggaatgat ccgggcaagc
     1981 gtggtgttca agccctgact caggcgggcg gtggcggcct gaatgcggcg gatgaaattg
     2041 agcagcccac gctgccactg gggcgcggga cgttcaaccg gctggcggcg cgggcggggt
     2101 gaggcggcag ccggagcagc agattctgcc gccgggcgct tgacctcaac ggttgggcgc
     2161 ggctcggctg gctccagggg ggcggtatcg ccggtaatat agaccgggcc agagggatgt
     2221 aattcggggg tgccggcaag ttcggcggca ggcgctgtgc ccggaacccg aggggcggca
     2281 gcccgcacct gacccttgcc ggtctgaaat tgaacaatca gagcctgcaa gtcggcgctg
     2341 gtttgcagca gcagccggcg ccggaaagcc tcccggctga gggttgagcc atcggcattt
     2401 gccagccagg attgcggcgg acgcggacag aagaccagca tgtcgccggg ttgaatggtg
     2461 gtggaataga agcgcgggat caggtcgtgc gagaggccca ggttgatgac atcggggtcg
     2521 tgatcgtcac ggacgggggc gcgggtgagc gagaaggagt gggtgacacc agcatgggca
     2581 atgacgattg aatcgccgtg caggacggcc aggttgagca caccgccaag ctgaccggcg
     2641 ccaggggttt gctgcttgta ctgcagcagg gtttcgttga tggtacgaac cgcctgccgc
     2701 aagccagcag tgatggagcc tggggcggtg aaataggcat tggatagttc tgccagccag
     2761 gttttgatga gatctggcgg gggtgtttgg ccatcttcaa atgtcagcag ggcgcagagc
     2821 aaatcctggg tacgggcgcg cgctgcaccg cggggtgcgg cctcggccag caggcctggc
     2881 aggctgccct ggggctgccc ggttttgaga ttgagtgcat agaagtagag gtcaaacaca
     2941 gccggtgtga atccattctg gcggtacgcc agggtcaagt ggggataggt cgggctgcgg
     3001 caggttcaca gcagcccggc aggatggttg caagatgcag gcctagctgc tgcggcgatc
     3061 tacggcgcgc tggccgcccc atttttgaat aatctgatca tcatactggt cggccgtgta
     3121 aaccacagcg aggacggagc tcttcagctc ttccatgtcg caggtggagc ccacaatggt
     3181 atgttcgaag aagatgtcgt tgtcctgatc cacgccaaag gcaccgaagc gcatgcggtc
     3241 attctcgcgc agcaggaagt gcatcagatc cggtgtcagt tcaacgccag tgaccacata
     3301 ggcgcgggta gtgatggtgg catcgtcttc gccccaggcc atcacgccaa tttggacgta
     3361 ggcagagccc acagcaagac caaaggtggg ggcatcttca cgggcatggg caaaggtgcc
     3421 aaagagttct ttgacccagg gaacaagttt ctcataggtc gcctgatgag cagcagtttg
     3481 aaattccatt tgatcctcct gactgattta ctggggtttg gatacggcaa tttcaccgtc
     3541 ttgaattttg acggtgacgg tctctttttg ggctgcgcgg gcgtgcggca agtgctggat
     3601 cagcgccggc gtgaggatgt tatccaccca gcgctcccaa tccgaatcgc tgaagccttg
     3661 cgagagcgcc tccatcagcc actcgatgag ggtgttgtcc cactgaattt gaagaccctg
     3721 tttggagtac cgctgggcga cctcattcag caggttttgt tccaaccact ctggagaaat
     3781 gccttcttcg ccatgagcgc caaacagcac caaatcgagt tgagaggcaa ggtcttcgcc
     3841 aatgctggcg cgcacggctg actcgatatc gtcggtgcca atttcagccg tttgctggaa
     3901 gaagcccagg gagcgctggg tttcaatttc aatatcggca gtaatcagaa caatggtatc
     3961 gctgaggtgc atgagcttgc cgcggccatc catcaggtta ccatcgcgga aggcctgggt
     4021 gagcacctca cgaatatacg gatggcagga gtcaacgttt tcaaagcgca gtacgcacca
     4081 ggggatctgc gccaggcggt gcagaggcag gctgtcggta tagccgatgt agccgggcgg
     4141 tgcgccaaca agcaggttca cgtcttcggg gtgcaggaag cgggcaaaat caatggcaat
     4201 gacgcgatct tcgccgccat agactgcctg ggcaatgacc tgcgccagca tctcgctgtt
     4261 ttctttggca tcgcgggtga gcatgatgat ggcgttggga cggtcagagc gcatatcgag
     4321 gccacgcatg gtgacctgca ggcggttaat cagcgcggcg gtttcagcct tgccgagcag
     4381 gccctgcccg gtgagttgtt tttccagctc agacaggcgg tcttccaggg ccaggggcat
     4441 gccgaccatg cgctgggcga cttcctgggc atcgtcaata tccacggctt ctttgccctg
     4501 ggtgacggca tgcgccacac actgctccag caggtctaca gctttgtcgg ggaactggcg
     4561 gttgcgcatg tactggtcgc cgaactgcag cagccaggca aggatttcat cggagatggt
     4621 gatggtgcgg gatttgacga actcgtctcg cagggtttga atgacctgca tggtctcatc
     4681 aagcgacagc tcatgcacac gcacagggtt aaagcgccgt tccagggcgg catcgttttc
     4741 aataaagcgg cggtattcat cgttggtggt agcggcaatc acggcaatat caccccgcgc
     4801 cagggatggc ttgaggaggc tgcccatgtc cgaggtgccc aacatgccgc cagagcccat
     4861 gatggtgtga atttcatcaa tgaagaggat gatgccgggc tgcgaggctt cacgcaccac
     4921 ggcctggacg cgtttttcaa gctcgccgct catgctggcg ccagccacca gaaccgaggg
     4981 ctggatggcg atgatgcggc agtccttgag catttgcggc actttaccgg tcacaatcat
     5041 ctgcgccagg ccttcaacaa tggcggtttt gccaacacca gccgggccaa ccagcaccgg
     5101 gttgcgtttg ctgcgccggc aaagggtttc aacggtcatt tcaatttcgt catggcggcc
     5161 aatgatgggg ctgagcttgc cttcgcgggc catttgggtc aggtcccgcc cgtacttatc
     5221 gagcgcgggg gtggctgaag caccgctgcc gggcttgaaa ggttcgccac cgccagacgg
     5281 ttcggcgctt tccgatgagc tgccgtgcag ttgcagggaa ccccactcgg ccaccttgta
     5341 gccagcccgg gtgaggatga tggtggcgac atcgcgctcg gaagcctgca tcttgccgcg
     5401 ctgctgggcc tgggc
//
LOCUS       DAOPVS010000125         5401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15162_length_5401_cov_55.911897, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000125 DAOPVS010000000
VERSION     DAOPVS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15162_length_5401_cov_55.911897"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKL11_08945"
     CDS             complement(<1..272)
                     /locus_tag="PKL11_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNZ13767.1"
                     /translation="MTTAGYREISHTADWALQVWAPDLSGLLVQAARGMTALLDLQLA
                     EAPRLEHLLTLDAADPESLLVSFLTEILYINEQEHLGFDAFQVSLDG"
     gene            312..1064
                     /locus_tag="PKL11_08950"
     CDS             312..1064
                     /locus_tag="PKL11_08950"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNZ13768.1"
                     /translation="MKLPFDQIEAKLQALVERRLTLFKRVEPQHQIIHALAEALNAVD
                     NCQNAPELFTIHLHPEGAAYWQSHPEALAELGRALQATSQGTQCPLVTAPLIRVLSDP
                     TLAPGDVRVETGQRQVSNSETAVLRIPTDELPPPTQPSPRKAYLILPGDQVYWLVLSV
                     VNIGRRGDNQLVLSDLQISRNHAQVRLVHGQFVIFDLDSTSGTFVNGNPVREHALRPG
                     DVITLGSIALIYGEDVPDTPTAGNQTAAMIDK"
     gene            1061..1498
                     /locus_tag="PKL11_08955"
     CDS             1061..1498
                     /locus_tag="PKL11_08955"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNZ13769.1"
                     /translation="MTAILTLIFRILLAAALYGFLAWALITLWRDLQMQTQILQPRKP
                     PALHLAVDLGGELIESDLEQPEIILGRDPGCDLQVPHETVSGRHARLSYHHKQWWVED
                     LQSTNGTYLNEDRILTPTVLTIGDELLLGQVSIQISSGKANGN"
     gene            1525..2400
                     /gene="mtnP"
                     /locus_tag="PKL11_08960"
     CDS             1525..2400
                     /gene="mtnP"
                     /locus_tag="PKL11_08960"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNZ13770.1"
                     /translation="MNHAEPVLGIIGGSGLYQFEGLTQVETLELETPFGKPSSPIVIG
                     NIAGKRVAFLARHGIGHFISPTEVNYRANIYAFKALGVPRIVSISACGSLRDDYAPGE
                     IVIPDQLVDFTRMRARSFFGEGLVAHIGVADPFCPDLSAKLFSAVNQAGVLAHKGGTL
                     ITIEGPRFSTRAESNLFRSWGMSIIGMTACPEAFLAREAEICYATMAHVTDYDVWHVS
                     EEAVSVEMVVRTLLKNTEGAQKAVISLVKGLFDTEPACGCGHALETAIITNPAHVPGE
                     TRQKLALLTEKYIQP"
     gene            2397..5018
                     /locus_tag="PKL11_08965"
     CDS             2397..5018
                     /locus_tag="PKL11_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNZ13771.1"
                     /translation="MKFFPISGARQGQIRSRLMTLAAGFVLLYGLILTLAPAVRLQSW
                     LVDYRWQHWLGVIVWLVGFAWLHRQINERLPESDPYLLPAAALLTGWGLLTIWRLNEA
                     YGLRQTIWLAVSLGIFGGGLRLKPLFEILRRYKYLWVTGMLVLVTLTLVLGVYPGGSG
                     PGLWLGCCGVYFQPSELLKLLLIAYLAAYLGDGLRGQHQLLRLLQPTLLVVGATLLVL
                     VLQRDLGTATLFILLYALIVYLATGRRSVILLSVGAMAAAALAGYYFFGVIQARVNTW
                     LNPWGDPAGTSYQIIQSLMAIAAGGAMGTGPGLGSPGLVPVAISDFVFAAIVEETGWL
                     GAIGLLLLIGLMLTRGLRAAVQAGSTYARLLAAGISVYFCVQSVLILAGNLSLAPLTG
                     ITLPFVSYGGSSMLTAFIMLLLLIRISGKNEGDPLPIHAPRPYLLTAGAMLVALAVVA
                     GGVIWWNVFQADALFIRNDNFRRSIADRYVERGALLDRNNNTLAMSEGERGNFQRNVL
                     YPDLSATLGYTHPSFGQSGLEASQDDYLRGLRGNPASDIWLTQLLYNHPPQGLDVRLS
                     LDVSIQREADALMEGLTGGLVLMNAETGEVLALATTPTFDANQLDELEETWSDDKRAP
                     LVNRVTQGLYPPGAALGPFFLAARLGQNPILSDTPPTDYYAAGQRYACAQMVGLDASW
                     GEAIASGCPAAGIALFENADLEAMQVLYERLGLLQAPDFDLVMAGPQPPEVNASAAEI
                     ITAQDGMKVTPLQMALAAAALTNDGKRPGPRLAMSVLTPHQGWVALPVVAAEQALPTS
                     GASAAVTKLKISEMPAWETVAVVRGTSQEVTWYLAGTTPTWQGVPLTVVVLLEENNAT
                     RAVEIGQSLLQAVMEGK"
     gene            5020..>5401
                     /locus_tag="PKL11_08970"
     CDS             5020..>5401
                     /locus_tag="PKL11_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13772.1"
                     /translation="MSNKRGLPDLAQAVTSGCLLPAFLWLWIAVALAGIVTAVIALTK
                     QPVDGNAIAPDWAYIATILVFGLYLISIIGILLEKEAGVSGFLLASVLLYGLNVLTGA
                     LPGESMGIFGGVILLLFLLRLDEWQ"
BASE COUNT         1072 a         1367 c         1720 g         1242 t
ORIGIN      
        1 ccatccaagg aaacctgaaa ggcatcaaaa ccgagatgct cctgttcgtt gatatagagg
       61 atctcggtca ggaaagaaac cagcaggctt tcggggtcgg cggcgtccag ggtcagcaaa
      121 tgctccaggc ggggtgcttc ggccaattgc aggtcgagca gggcggtcat gccacgggca
      181 gcctgcacaa gcaagccaga caagtctggc gcccaaacct gcaaggccca atcggcagtg
      241 tgagaaattt cacggtatcc agcagttgtc attttcataa gtcctctacc aacctgttat
      301 aattatatcc gatgaaactc ccctttgatc aaattgaagc aaaactgcag gccctggtcg
      361 agcgccgcct gacgctgttc aagcgggtgg agccccaaca tcagatcatt cacgcgctgg
      421 ctgaggcgtt aaacgcggtg gataattgcc agaatgcgcc ggaactgttt accattcatc
      481 tgcacccgga gggagcggct tactggcagt ctcacccaga ggcgctggca gaactggggc
      541 gggcgctcca ggccacctcg caggggacgc agtgtccact ggttacagcg cctttgattc
      601 gggttttgtc tgacccaacc ctggcgccgg gcgatgtgcg ggttgaaacc gggcagcggc
      661 aggttagcaa cagtgagact gccgtgctgc ggatcccgac tgatgaactg ccccccccga
      721 cgcagccatc cccgcgcaag gcgtatttga ttttgccggg tgaccaggtg tactggctgg
      781 tgctttcggt tgtcaatatt ggccgacggg gcgataatca actggtgctc agtgacctgc
      841 agatctcccg taatcacgcc caggtgcggc tggttcatgg tcaatttgtc atttttgacc
      901 ttgattcgac cagtggaacg tttgtgaacg gcaatccggt gcgcgaacac gctctccgtc
      961 cgggagatgt gatcacactg ggcagtattg ccttgattta cggggaagat gtgccggaca
     1021 ctcctacggc gggaaatcaa accgcagcga tgattgacaa atgaccgcaa ttctcaccct
     1081 tatctttcgc attttactgg ctgctgcact ttatggcttt ctggcatggg cgctgattac
     1141 cctgtggcgc gatttgcaga tgcaaaccca gattttgcag ccgcgcaaac ccccggcgtt
     1201 acatctggcg gtggacctgg gcggcgaact gattgaaagc gacctggaac agccggaaat
     1261 tattctgggg cgcgacccgg gctgcgactt acaggtgccc catgaaactg tatcggggcg
     1321 gcatgcgcgc ctgagttatc atcacaagca gtggtgggtt gaagacttgc aatctacaaa
     1381 cggcacttat ttaaatgaag accggatttt gacccccacc gtcttaacca tcggcgatga
     1441 actgctgctg gggcaagtca gcatccagat cagcagcggc aaagccaacg ggaactgaga
     1501 aacagatttt ttaaggagat agacatgaat cacgcggaac ctgtgcttgg catcattggc
     1561 ggttcagggc tgtatcaatt tgagggcttg acccaggttg aaacgctgga actggaaaca
     1621 ccttttggta aaccgagctc gccgattgtg attggcaata ttgccggaaa gcgggtggcc
     1681 tttctggcgc ggcacgggat tgggcatttc atctccccca ccgaggttaa ttaccgcgca
     1741 aatatctacg cgttcaaagc actgggggtg ccgcggattg tgagcatcag cgcatgtggg
     1801 tcgctgcgcg atgactacgc ccccggagag attgtcatcc cagaccagct ggttgatttt
     1861 acccggatgc gcgcccggtc gttctttggc gaagggctgg tggcacatat cggcgtggca
     1921 gaccccttct gccccgatct ttcggccaag ttgttttcag cggtgaatca ggccggcgtt
     1981 ctggcacaca agggcggcac actgattacg attgaggggc cgcggttttc aacccgggcc
     2041 gagtctaatc tcttccgctc gtggggaatg tcaatcatcg gcatgaccgc ctgccccgag
     2101 gcttttctgg cgcgcgaggc agaaatctgt tacgcgacta tggcgcatgt caccgattac
     2161 gacgtgtggc acgtcagcga ggaagcggtc agtgtggaga tggttgttcg gacgctgctt
     2221 aaaaatactg agggcgctca aaaagcggtc atcagcctgg tgaagggtct gtttgatact
     2281 gagccggcct gcggctgcgg tcacgcgctt gaaaccgcca tcatcaccaa cccggcgcat
     2341 gtgcccgggg aaacccggca gaaactggcg ttgctgacgg agaaatatat tcagccgtga
     2401 aattttttcc aatctcggga gcgcgccaag gacaaattcg cagccgcctg atgactctgg
     2461 cggccgggtt tgtgctgctc tacgggttga ttttgacgct tgctccggcg gtgcgactgc
     2521 agtcttggct ggtggattac cgctggcagc actggctggg ggtgatcgtc tggctggtgg
     2581 gctttgcctg gcttcatcgc cagattaatg agcggctgcc ggaatctgat ccttacctgt
     2641 tacctgcggc cgcattatta accggttggg ggttgctgac catctggcgg ctcaatgaag
     2701 cgtatgggct gcgtcagacc atctggctgg cggtgagtct gggcatattt gggggcgggc
     2761 tgcggctcaa gccattgttt gagattttgc ggcggtataa gtacctgtgg gttacaggga
     2821 tgctggtgct ggtgacatta acgctggtgc tgggtgttta ccccggaggc agcggccccg
     2881 gattatggct gggctgctgc ggggtgtatt ttcagccctc tgaactgctc aagctgctgt
     2941 tgattgctta tctggcggcc tatttgggcg atggtctgag agggcagcat cagttactgc
     3001 ggctgctgca accgacgctg ctggtggtgg gagccacatt gctggtgctg gttttgcagc
     3061 gcgacctggg gacggcaacg ctgttcattc tgctttatgc tttgattgtc tatctggcaa
     3121 ccgggcggcg gagcgtgatc ttgctcagtg tgggggcaat ggcggcggct gcgctggcag
     3181 ggtattattt ctttggggtt attcaagcac gggtcaatac ctggttaaat ccgtggggcg
     3241 acccggcagg aacttcttac cagattattc agtccttgat ggcgattgcg gccgggggcg
     3301 caatgggaac cgggcccggg ctgggcagcc cagggttggt gccggtggcg atttcggatt
     3361 ttgtgtttgc ggccattgtt gaagagactg gctggctggg agccattggc ctgctgctct
     3421 tgattgggct gatgctcacg cgtgggctgc gagccgcagt gcaagctggc agcacttatg
     3481 ccaggctgct ggcggcgggg atcagcgtct atttctgcgt tcagagcgtg ctgattctgg
     3541 ctggaaattt gagcctggca ccgctgaccg ggattacgct gccgtttgtg tcttacggtg
     3601 ggtcgtctat gctgacggct tttatcatgc tgctgctgtt gatccgaatc agcggcaaaa
     3661 atgaggggga tcccctgccg atccatgctc cgcggcccta cctgctgacc gctggggcaa
     3721 tgctggtggc actggcggtg gtggcaggcg gcgttatctg gtggaatgta ttccaggcag
     3781 atgccttgtt tattcggaat gataacttcc ggcgctcgat tgctgaccgt tatgtggagc
     3841 gcggcgcatt actggatcgt aacaataata cgctggcaat gagtgagggg gagcgcggca
     3901 attttcaacg gaatgtgctt tatccggacc tgagcgccac acttggctac acgcatccca
     3961 gttttggtca aagcggattg gaggccagcc aggatgatta tctgcgcggg ctgcgaggca
     4021 acccggcatc ggatatctgg ctgacgcaac tgctttataa tcacccgccg caaggtctgg
     4081 atgtgcggct gagtctggat gtgagcatcc aacgcgaggc agatgccttg atggaaggac
     4141 tgactggcgg actggtgctc atgaatgctg aaaccgggga agtgctggcg ctagcgacaa
     4201 cgccgacatt tgacgccaat caactggatg agctggaaga aacctggtcg gatgataagc
     4261 gcgccccgct ggtaaaccgg gtgacacagg ggttataccc accgggagcg gcgctggggc
     4321 cgttcttcct ggcggcacgg ctggggcaaa accctatatt atcagatacc ccgccgacag
     4381 attattatgc ggcaggtcag cgctatgcct gcgcacagat ggtgggcctg gatgcaagct
     4441 ggggcgaagc cattgccagc ggctgcccgg ctgccggaat tgctttattt gaaaacgctg
     4501 acctggaggc aatgcaggtg ctgtatgagc gcctgggttt gctgcaggcc cccgattttg
     4561 acctggtgat ggccgggcca cagcctcctg aggtgaatgc gtcagcggct gaaatcatca
     4621 cggctcagga cggtatgaaa gtgacgcctt tgcaaatggc actggcggca gccgccctga
     4681 caaacgacgg gaagcgccca ggaccgcggt tggccatgtc ggttttgacg ccgcatcaag
     4741 ggtgggtggc gctgccggtg gttgcggctg aacaggcgct gcccaccagc ggggcctcgg
     4801 cagcggtgac gaagttgaaa atcagcgaga tgccggcctg ggaaacagtg gcggtggtga
     4861 ggggaacatc gcaagaagtg acctggtatc tggctggcac aacgcccacc tggcaagggg
     4921 tgccattgac ggttgtggta ctattagaag agaacaatgc aacgcgcgcg gtagaaattg
     4981 gacaaagtct cttgcaggca gtgatggagg gcaaatgagg tgtccaacaa acgcggttta
     5041 ccggatctgg cgcaggctgt aaccagcggt tgtctgctgc cggcgtttct gtggctctgg
     5101 attgctgtgg cattagcggg gattgtgaca gcggtcattg cattgacaaa acagccagtg
     5161 gatgggaatg caattgcgcc agactgggcg tatattgcca caatcctcgt ttttggctta
     5221 tatctgattt cgattattgg catcttgctt gaaaaggaag cgggagtatc cggttttctc
     5281 ctggcatcgg tactgttata tgggttgaat gtgctgaccg gcgctttacc gggcgaatcc
     5341 atggggattt tcggcggggt gattttactg ctatttctct tgaggctgga tgaatggcaa
     5401 c
//
LOCUS       DAOPVS010000126         5394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15196_length_5394_cov_59.796217, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000126 DAOPVS010000000
VERSION     DAOPVS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15196_length_5394_cov_59.796217"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..962)
                     /locus_tag="PKL11_08975"
     CDS             complement(63..962)
                     /locus_tag="PKL11_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ13773.1"
                     /translation="MPASKQTWNWKKILRWAGTLASLGLFVYLLARQNWPQLWQALSR
                     LPVWAVGLAFSLYLSGQAANALRWWILLRSQKVTISYLQALQLIFAGAFASNFLPSTI
                     GGDALRMLGLFQYTTQRVMAVASVLLDRILNVLAYVTVAPFALGVALPQDLTILGFFI
                     TLPGWLRRILDKLLEAFRLWRQQPAALIRAFGVSWLSIFVIFLALFTLARGLGIPVAL
                     HQVIGINVVTYFLTLLPISVNGYGVREIAITGLYIQVGATLEQAAALAIISRLMLMAE
                     TLPGALWAPRLLPADKPSSDVVL"
     gene            complement(966..2015)
                     /locus_tag="PKL11_08980"
     CDS             complement(966..2015)
                     /locus_tag="PKL11_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13774.1"
                     /translation="MPLVDLSFSFHQTVGLVIETDDVLAREFFQAEYGPHAGPVGSLP
                     TIHLRFQRQEALLSVPAGYTFHAHKIVARWAYRVAWRSDGLSLDCLGNRFSIPMIHHM
                     LVHPSLRLLAARQGVLLLHAGAVSCRERSLLFTGKGGAGKTTTTALLMAYSPQPWSPH
                     ADDYTFLGAGPVSYAYLTRSHLYSDLLRWIPSMRSHLSPGERFRLSILGRVRAWSGER
                     IKWPVRVSLNRLWPTRPFDLQSRPSGLIFLRRGPVDRPTLEPLIPGEAELNELLEMNF
                     YEARHYLALLNQCAGMSESQLSEWRQQEGRLINRSLQEIPVYRLTLPTRKISAAEFGP
                     HLADLLDSQFAFSEV"
     gene            complement(2002..3138)
                     /locus_tag="PKL11_08985"
     CDS             complement(2002..3138)
                     /locus_tag="PKL11_08985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ13775.1"
                     /translation="MRILVLTHEYPPIGGGGGRVAEDLCTGLASRGHDVRVLTAHYRT
                     LALEERRDGVYIQRLTSNRRLPYEASFGAMARYVWRAFIEGQRLVKQWRPDVIHVHFA
                     VPAGAAAYWLHQWTRVPYVLTAHLGDVPGGVPEKTDRWFRWVFPFTPGIWRSASRVVA
                     VSEFTSQLASRHYRVPIQVIPNGVDLDAVSPASLQPNQPPRLVFAGRFVEQKNPLYVV
                     RALSELRDLPWNCTMIGDGVLRPAVENEINRLNLKDRFTLTGWITPQQVLDWYNRSDI
                     LFMPSLSEGLPVVGVQALAMGLALVVGKAGGFIELVEADRNGRLIDPNTLAAGVAALR
                     QLLADPAHLAQCRINSRELAHRFDLNDVVSSYEKTLTEAANAPR"
     gene            complement(3138..4118)
                     /locus_tag="PKL11_08990"
     CDS             complement(3138..4118)
                     /locus_tag="PKL11_08990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13776.1"
                     /translation="MDLSVVIPVYNEEENLHLLFDAVTQALQPLNRTWEVILVDDGSR
                     DRSPELLEELAAKDPDHFRAVILRRNFGQTAAIAAGIDHSAGDVIILMDADLQNDPAD
                     IPMMLEHIDAGYDVVSGWRIQRQDTYLTRTLPSHMANWLISKVTGVNLHDYGCTLKAY
                     RREVLTGFRLYGEMHRFIPVYANQVGAKIIEVKVHHHPRKFGKAKYGLERTFKVVLDL
                     LTVKFLSSYANKPIYLFGGAGAVLIALSAIGLLYLFIRRMIPPYTTVFDSPIFQMSTM
                     IAILGFQSILMGLIAELLARTYHESQRKPTYTVRKRIGIQSKDSNSAGES"
     gene            complement(4156..>5394)
                     /locus_tag="PKL11_08995"
     CDS             complement(4156..>5394)
                     /locus_tag="PKL11_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13777.1"
                     /translation="DAWQRDQQSWQLAQSDQALFSAFGSEFYSDQYGGLLWLGSLIYR
                     FLSPDAQRPMLMVILGAFTAAVGAAFLIRAVQSRFDPSIALVAGWIYALYPESILQGA
                     SQMREPFLMGLSAIAWWAVIAIPRTPIRWHPLAALIAASLGLMVLSWRAALPILLAIS
                     LWFVVDTLLDRMKSSTRWVMLAVLAVAFLVLAALSWRWFSEAGGWDMRVLEMSSGMLT
                     RQLSKLPESLQFVFIIFYGLTQPVLPAAIFETALPIRTAIAIGRALGWYALAPLLISA
                     GVSLWRIPDRTQRRLMMLAAAFSAAWIFVSSFRAGGDLWDNPRYRTILIIWLALLAAW
                     AWVWARQKRSPWLWRILAVEGIFLAFFTHWYLGRYLSAWPKMPLFPMLGLIALGAVLV
                     IGGGVIWDRWRNHHPPRKTL"
BASE COUNT         1397 a         1428 c         1623 g          946 t
ORIGIN      
        1 gtccaggctg aacggatcca aggttccagc tttgcttcca atcgtttcat aggcgctcgc
       61 aattatagca cgacatctga cgagggttta tcggcaggga gcagccgggg agcccacaag
      121 gcgccaggca aggtttcggc cataagcata aggcgggaga taatcgccag agccgcagcc
      181 tgttcgagcg tggcgccaac ctgtatatac aggccagtga tggcaatttc acgcacgccg
      241 tagccgttga cagagatggg taaaagagtc agaaagtaag tgacgacatt gatgccgatg
      301 acctgatgca gtgcaaccgg aatgcccagg ccgcgcgcca gcgtgaacag cgccagaaaa
      361 atcacaaaga ttgagagcca tgaaacgcca aacgcccgga taagcgccgc cggctgctga
      421 cgccagagcc taaatgcctc taacagctta tcaagaattc ggcgcagcca gcccggcaga
      481 gtgatgaaaa aacccaggat ggttaaatct tgaggcaagg caacccccag ggcaaaaggg
      541 gcaacggtta cataagccag cacattgagg attcgatcca agagcactga ggccacagcc
      601 atcacgcgct gcgtggtata ttgaaaaagc cccaacatcc gcagggcatc accaccgatg
      661 gtcgagggca agaagttgga agcaaacgcc ccggcaaaaa tcaattgaag cgcctggaga
      721 taggaaatgg tcactttttg cgagcgcagc aaaatccacc agcgcaaagc gttggcagcc
      781 tgccctgaca aataaaggct aaaggcgagg cccaccgccc agaccggtag gcggctgagc
      841 gcctgccaaa gctgcggcca attttggcgc gccaagagat aaacaaacaa tcccaaactg
      901 gccaaggttc cagcccagcg aagaattttt ttccaattcc aggtctgttt tgaggcaggc
      961 actgtttaga cctcggaaaa agcaaactgg ctatccagca aatcagccag gtgggggcca
     1021 aattcggcgg cagatatttt gcgggtgggt aatgtcaggc gataaacagg tatctcctgc
     1081 aaagaacggt taatcagccg cccttcctgt tgccgccact cggacaactg tgattccgac
     1141 ataccggcgc actgattcag cagggccaga tagtggcggg cttcatagaa gttcatttcc
     1201 agcagctcat ttaactcagc ctcgccggga atcaacggct ccagcgttgg gcggtctact
     1261 ggaccgcggc gcaggaaaat caagcccgac ggccgggatt ggaggtcgaa gggacgggtg
     1321 ggccaaaggc ggttgaggga aacccgcaca ggccatttga tgcgctcgcc cgaccaggcg
     1381 cgcacacggc ccagaatgga tagcctgaaa cgctcacccg gtgaaagatg gctgcgcatg
     1441 gaaggaatcc agcgcaggag gtctgagtag agatgggaac gggtgaggta ggcgtaactg
     1501 accgggccag cacctagaaa ggtataatca tcggcgtggg gagaccaggg ttgaggactg
     1561 taagccatca gtaatgcagt ggttgtggtc ttgccagcgc cgcccttgcc cgtgaataag
     1621 aggctgcgct cgcggcaaga gactgcaccg gcatgcagca ataagacgcc ctggcgggca
     1681 gccagcaggc gcaaactggg gtggaccagc atgtggtgaa tcatcgggat gctgaagcgg
     1741 ttgccgaggc agtccagtga gaggccatcc gagcgccatg ccacacgata agcccaacgg
     1801 gcaacaattt tatgggcgtg aaaagtgtac ccagccggga cgctcagcag agcttcctgt
     1861 ctctgaaagc gcaggtggat tgtagggaga cttccaacgg gcccggcatg gggaccatat
     1921 tcggcttgaa aaaattcgcg cgccagaacg tcatcagtct caattacaag gccgacggtc
     1981 tggtgaaaag agaaagataa atcaacgagg ggcattggct gcttcagtta acgttttttc
     2041 gtaagatgaa acgacgtcgt tcaagtcaaa gcggtgggca agttcccggc tattaatccg
     2101 gcactgcgcc agatgggcag ggtcagccag caattggcgc aaggcagcta ccccggccgc
     2161 cagggtgttg gggtcgatta aacggccatt gcgatctgcc tcgaccaatt cgatgaagcc
     2221 gccagctttg cccacaacca gtgccagccc catggcaagc gcctggacac cgacgacggg
     2281 caagccctct gacagcgaag gcatgaataa aatatcgctg cggttatacc agtccaatac
     2341 ctgttgggga gtgatccaac cggtgagggt aaagcggtcc ttgagattga ggcggttaat
     2401 ttcgttttca actgccgggc gcaaaacacc atcgccaatc atggtgcagt tccaggggag
     2461 atcacggagt tcggacagcg cccgcactac atacaggggg tttttctgct ctacaaaccg
     2521 accagcaaac accagacggg gcggctggtt gggctgcagg ctggcagggc taactgcgtc
     2581 caaatcaacg ccgttgggga tcacctgaat gggaacgcga tagtgccggg atgccaactg
     2641 gctggtaaat tcgctgacgg cgaccacgcg gctggcagaa cgccagatgc ccggcgtaaa
     2701 tgggaaaacc cagcgaaacc agcggtctgt tttttcggga acgccaccgg ggacatcccc
     2761 aaggtgggcg gtcaaaacat aggggacgcg cgtccattga tgcagccaat aggcagcagc
     2821 accagcaggc acagcaaagt gaacgtgaat gacatctgga cgccactgtt taaccagacg
     2881 ctgaccctcg ataaaagccc gccaaacgta gcgagccatg gcgccaaagc tggcttcgta
     2941 gggcagccgc cgattggagg tcaggcgctg gatgtagacg ccatcacggc gttcttccaa
     3001 cgccagggtg cggtaatgtg cggtgagcac gcggacatcg tgtccgcggc tggccagccc
     3061 ggtgcataaa tcctccgcca cacgcccgcc gcccccacca atgggggggt attcgtgcgt
     3121 cagcaccaaa attcgcatca agattctcca gcggagttag aatccttgga ttgaataccg
     3181 atgcgtttac gaacggtata ggtcggtttg cgctgtgatt cgtgataggt gcgcgccaga
     3241 agctcggcga tcagacccat gagaatggat tgaaaaccga gaatggcgat catggtggac
     3301 atttgaaaaa ttggtgaatc aaaaacagta gtgtagggcg gaatcatgcg ccggataaag
     3361 agatagagta ggccaatggc gctcaaggcg atcagaactg ctccggcacc gccaaacagg
     3421 taaatgggtt tattggcgta gctgctgagg aatttgaccg tgaggagatc cagaaccacc
     3481 ttgaaggtac gttcaagacc gtatttggct ttgccgaact tgcgggggtg gtgatggact
     3541 ttgacttcaa tgatttttgc gcctacttga ttggcataca ccgggataaa gcgatgcatt
     3601 tcgccgtaga ggcgaaaccc ggtcaggact tcgcggcggt aggctttgag tgtgcaacca
     3661 taatcatgta aattgacgcc ggttactttt gaaatcagcc agtttgccat gtgggacggc
     3721 agggtgcggg tcagataagt gtcctgacgc tgaatgcgcc agccgctgac cacatcgtac
     3781 cccgcgtcga tatgttcgag catcatgggg atatcggcgg gatcattttg caaatcagca
     3841 tccatcagga taatgacatc gccagcagaa tgatcaatac cggcggcaat agcagcggtc
     3901 tgaccaaagt tacggcgcag gatgactgcc cggaaatggt ccgggtcttt ggcggcaagc
     3961 tcttctaaca gctcggggct gcggtcgcgg ctgccatcat ctaccaggat aacttcccag
     4021 gtgcggttga ggggttgaag cgcctgagtt acggcatcaa acaggaggtg gagattttct
     4081 tcttcgttat aaaccggaat gacaactgag aggtccatag gcctgctttc gttaatcggg
     4141 ttgattttga gccaattata acgtctttcg aggtgggtga tggttgcgcc agcgatccca
     4201 aatgacgccg ccgccaatga ccagcaccgc gcccagggca atcagcccca acatagggaa
     4261 gaggggcatt ttaggccagg cagagaggta gcgccccaga taccagtgcg taaagaaggc
     4321 caggaagatg ccttcaacag ccaggatacg ccacaaccag gggctgcgtt tctgacgggc
     4381 ccagacccag gcccaggcag ccagcagcgc cagccagatt atcaggatcg tacgatagcg
     4441 cgggttatcc cacaggtcgc cgccggcgcg gaacgacgaa acgaatatcc aggcagcaga
     4501 aaacgcggct gccagcatca tcagacggcg ctgcgtccgg tctgggatgc gccagaggga
     4561 gacgcctgcc gagatcaaca aaggcgccag agcataccaa cccaacgcgc gcccgatggc
     4621 gatcgctgtg cggatgggaa gagccgtctc gaaaatagca gcaggtaata cgggttgggt
     4681 aagaccataa aaaatgataa aaacaaattg taaggattct ggcagtttcg acaactgccg
     4741 ggtcaacatg cccgaagaca tttccaagac ccgcatgtcc cagccgccag cttcgctaaa
     4801 ccagcgccag ctaagcgccg ccagcacgag aaaagcaaca gccaggacag ccagcatcac
     4861 ccagcgggtg gaagatttca tccgatccag cagtgtatct acgacgaacc agagggagat
     4921 tgcgagtaag attggcaagg cggcgcgcca ggataaaacc atcagaccca gagaggcagc
     4981 aattaaagct gcaagaggat gccagcggat gggggtgcgc gggatggcaa ttacagccca
     5041 ccaggcaatg gcgctgaggc ccatgaggaa tggctcgcgc atttgagagg caccctgcaa
     5101 aatgctctcg gggtaaagcg cataaatcca gccggccacg agggcgatgg atgggtcgaa
     5161 gcgggattga accgcacgga tcaggaaagc cgcacccacc gccgcggtaa aggcgcccaa
     5221 tatcaccatc aacatgggac gctgggcatc ggggctgagg aagcgataga tcaggctgcc
     5281 cagccagagc aagccgccgt actggtcgct gtaaaattcg ctgccaaagg cggaaaacag
     5341 ggcctggtcg gattgcgcca actgccagga ctgctgatca cgctgccagg catc
//
LOCUS       DAOPVS010000127         5364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15322_length_5364_cov_58.767941, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000127 DAOPVS010000000
VERSION     DAOPVS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15322_length_5364_cov_58.767941"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKL11_09000"
     CDS             <1..756
                     /locus_tag="PKL11_09000"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNZ13778.1"
                     /translation="ASFQEESAYRANFFISLLTSLLNLATGVLGIVVLFGQIDTVQGW
                     NLASTLALLGVYLTVSALRQLFIGPSLDALSGMDGEIWTGAFDFTVLRPVDTQFMASF
                     RKWRWFASIDLLLGLGVLVTAVIQLGVTLSLGQVLAFLLTLFSGMLILYAILLAFAAL
                     IFWSPGVLFTWVFDGIFQLGRYPLGMYPGWLKLILTWVVPVGVITTIPAQALLGSLTL
                     PGLLTSLGLALVLVTAASILFRVGLKRYASASS"
     gene            complement(827..1660)
                     /locus_tag="PKL11_09005"
     CDS             complement(827..1660)
                     /locus_tag="PKL11_09005"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNZ13779.1"
                     /translation="MSQNEFSWKSADGLAFYGREWRPDGQPRAVVVLVHGLGEHCGRY
                     AHVAEVFNAAGLGVVALDHRGHGKTEGTRGHGSYDGMGNDIAHLLEEAANRYPGLPCF
                     LYGHSLGGALVLYYTLTRKPVLAGVIATAPGLIPANPPAGAMMLAAKIASVVAPAMQI
                     DNNLDRTGLARDPEVEKKYSADPLVHGKISAKLGMDLINKGAWMIAHAAEFPLPLLLL
                     QGTADRLVSPTGAAAFAAAAPKDKLTYKTYEGYYHELHNEPEKVEVLADILDWINEQL
                     G"
     gene            1867..4272
                     /locus_tag="PKL11_09010"
     CDS             1867..4272
                     /locus_tag="PKL11_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNZ13780.1"
                     /translation="MADIPQALKEAPPRDNRAGCGLVGLFVFAIVWVLGSTILVQVVT
                     WVTEQALMEGSIGLPDVRWVIALGYAIMVLIPVAISTASVSLPRPKAIYRTWTMAAIF
                     ALFVVPARVIFFTQSQLAAVLQIMGMALYLMLLWRWLKPEKGGALRLIWLALIAGGLI
                     TLPWALWGALGSPMDILLGLVVALMFGVCAALTIQSGMLKVTQQDPTADQMQNVLMEG
                     LAASLTLAIMATGLGLCGAQLMLVFALPVLGWAVSTLTAAGSWQTRGQNWLTVSLLLA
                     LAAAGPLLWVDPDELALVITGTPGDLISFAAGATLGGLLMGLLLSIIGVSSYKRLRQA
                     AVLPAKVKIGAGLAWALVLIVYFLAGQPGFHGERLFVILNDQADLSPAAQIADPAARR
                     AWVYETLTAHANTTQGDIRKTLDTFNFAYQPYYLVNAIEVQGGPIIRWWLENRPEVDR
                     VLDSPRLRPLPADLPTASGSATPSNGPDWNLTMIHADQVWDQLGVRGDGIIIGQSDSG
                     VQGDHPELNDSYLGRDGQNDYRWYDPWFGSTSPTDIGGHGTHTLGSILGNQVGVAPDA
                     DWIGCVNLGRNLGNPALYLDCWQFMLAPFPQTGDPLTDGDPARGANILNNSWGCPEIE
                     GCDPSTFISATKALRSAGIFVVVSAGNSGYGGCGSVKDPPATDEFVYSVGAIDSNGSL
                     AEFSSIGPVVVDGSERTKPDIVAPGDGVPSSYPGSSYEVASGTSMAGPHVVGVVALMW
                     SANPDLIGEVEETIAILNETAQPYEGPYPECVTDRSAPNNAVGYGIVDAYAAVLRALQ
                     VHP"
     gene            4336..4908
                     /locus_tag="PKL11_09015"
     CDS             4336..4908
                     /locus_tag="PKL11_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ13781.1"
                     /translation="MSLDAPELTDAEALTRAADGDLDAFGVLYERYVTRIYNYIYYRT
                     GNPHEAEDLTARVFQRAARHIPNYRQMGVPFAAWLYRIAHNLVANWHRDRSRRREVPL
                     DDQPRLMMPIEHPESTIVRTQEMQTLLKAIRSLTPERQNLVIFKFVEQLSNAEIAVIM
                     GRTEGAIKSLYHRTLLALREELERLEKSGG"
     gene            4910..>5364
                     /locus_tag="PKL11_09020"
     CDS             4910..>5364
                     /locus_tag="PKL11_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ13782.1"
                     /translation="MLRIVTDGAVDCPAEWRDEYGIDVLPLYIQIGDQTLVEGVNVSV
                     DNFYGLVEKSQKPPKTSLPAPAQIVAFYHQIARPGDSILSIHIASKMSGTFAAVQLAA
                     REVASEFSIHVFDSGAGSAALGFMCREARRLERAGASIQKTLQRLEQAR"
BASE COUNT          922 a         1766 c         1452 g         1224 t
ORIGIN      
        1 gcttcctttc aagaggaatc cgcctaccgc gccaattttt tcatcagcct gctcacctct
       61 ttgctcaacc tggcaaccgg tgtgctgggc attgtggtcc tgttcggcca aattgatacc
      121 gtccagggct ggaatctggc ttctacgctg gcgctgctgg gcgtctatct cactgtcagc
      181 gccctgcgtc aattgttcat tggccccagc ctggatgccc tctccggcat ggatggcgaa
      241 atctggaccg gcgcctttga ctttaccgtc ctccgcccgg tggatacgca gtttatggcc
      301 agcttccgca aatggcgctg gttcgcctcc attgacctgc tgctcgggtt gggcgtcctg
      361 gtcactgccg tcatccaact gggcgtcacg ctctccctcg ggcaagtcct ggccttcctg
      421 ctgaccttgt tctccggcat gctcatcctg tatgccatcc tgctggcctt cgccgcgctc
      481 atcttttgga gtccgggtgt gcttttcacc tgggtgtttg atggcatttt tcagctgggg
      541 cgctacccgc tgggcatgta ccccggctgg ctcaagctga tcctcacctg ggttgtcccc
      601 gtgggcgtca tcaccaccat ccccgcccag gcgctgctgg gctcgctcac cctccccggc
      661 ctcctgacca gcctgggcct ggcgctggtc ctcgtcaccg ctgcctcaat cctgttccgt
      721 gtcggcctca aacgctacgc cagcgcctcc agttgagtgt cactacccgg tcgcagctta
      781 aacgcaaaag ccgccggttt gtccggcggc ttcttgtttt ctcggcttaa cctaattgct
      841 cgttgatcca atcaaggatg tctgccagca cttccacctt ctcgggctca ttgtgcagct
      901 cgtgataata cccctcatag gtcttgtagg tcagcttgtc tttcggtgcc gcagccgcaa
      961 atgcggcggc cccggtcggg ctcaccagcc ggtcggctgt gccttgcagc agcagcagcg
     1021 gcagcgggaa ctctgccgca tgggcaatca tccatgcccc cttgttaatc aagtccatgc
     1081 ccagctttgc agatatcttg ccatgcacca gcgggtcagc gctgtatttc ttttccactt
     1141 ccgggtcacg cgccaggccg gtccggtcca ggttgttatc aatttgcatg gctggggcca
     1201 ccacactggc aattttagcc gccagcatca tcgcgcccgc cggtgggttg gccggaatca
     1261 atcccggtgc ggtggcaatc acgcccgcca gtaccggttt gcgcgtcagg gtataataca
     1321 gaaccagcgc cccgcccagg ctgtgcccgt acaaaaagca tggcagcccc gggtagcggt
     1381 tggcggcttc ttcaagcagg tgtgcaatgt cattgcccat gccgtcataa ctgccgtgcc
     1441 cgcgtgtgcc ttcggtcttg ccgtgcccgc ggtgatccag cgccacaacg cccaaacccg
     1501 ccgcgttgaa cacctccgct acgtgggcat agcgtccgca gtgctcgccc agcccgtgca
     1561 ccagcaccac cacagcgcgc ggctgcccgt cagggcgcca ttcgcgccca taaaaagcca
     1621 gcccatcggc tgatttccac gaaaattcat tctggctcat cctgactcct tctggttcaa
     1681 aaaatcaaac cggataccaa cgcgtcccgg cttgggctta taatcaatcc acaatcagtg
     1741 tacagcaggt cagccaaatg ggcaagccag tccttgcccg gatttttgcc gggccgtgtt
     1801 acaatatttt gtattggctc agtgcgaata acatcttcag attcttttac caacaaaggt
     1861 gatctcatgg ccgacattcc ccaagcacta aaagaagcac ctccgcgtga taaccgggcc
     1921 ggttgcggcc tggtcggttt atttgtcttc gccatcgtct gggtactcgg cagcaccatc
     1981 ctggttcagg tggtaacctg ggtgaccgaa caggccctca tggagggctc gattgggctt
     2041 cccgatgtcc gctgggtcat tgccctgggc tatgccatca tggtcctcat tccagttgct
     2101 atcagcactg cctctgtctc cctgccgcgc cccaaggcca tttaccgcac ttggaccatg
     2161 gccgccatct ttgcgctctt tgtggttccg gcccgggtga tctttttcac ccaatcacaa
     2221 ttggctgctg tcttgcaaat catgggcatg gcgctctatc tgatgctgct ctggcgctgg
     2281 ctcaaacccg aaaagggcgg tgccctgcgc ctcatctggc tggcgctcat cgctggtggc
     2341 ctgattactc tgccctgggc gctctggggc gccctgggtt cccccatgga tatcctcctg
     2401 ggactggtcg tggcgctcat gtttggcgtt tgtgctgccc tcaccatcca atctgggatg
     2461 ctcaaggtca cccagcaaga ccccaccgcc gaccagatgc agaacgtcct catggaaggc
     2521 ctggctgcca gcctgacgct tgccatcatg gccactggcc tcgggctgtg cggcgctcag
     2581 ctcatgctgg tctttgccct tccggtgctg ggttgggcgg tttccacgct cactgctgca
     2641 ggcagttggc agactcgcgg ccagaactgg ctgaccgtct ccctgcttct ggcgctggcc
     2701 gcggcgggcc ctctcctctg ggttgacccc gacgagctcg ccctggtcat caccggcacc
     2761 cccggcgacc tgattagctt tgccgccggt gccacccttg gcggattgct gatgggtctg
     2821 ctcctttcca taatcggggt ttcttcgtat aaacgtctgc gtcaggctgc cgtgttgcct
     2881 gcaaaggtga aaatcggcgc cggtcttgcc tgggccctgg tcctgatcgt ctatttcctt
     2941 gctggtcagc ccggcttcca cggtgagcgt ctgtttgtca tcctgaatga ccaggccgat
     3001 ttatcgcctg ccgcccagat cgctgacccc gccgcgcgcc gcgcctgggt ttatgagacc
     3061 ctcaccgccc acgccaatac cactcagggt gatatccgca aaaccctcga taccttcaat
     3121 ttcgcctacc agccctacta cctcgtcaat gccatcgaag tccagggcgg ccccatcatt
     3181 cgctggtggc tcgaaaaccg ccccgaagtg gatcgtgttc tcgacagccc gcgcctgcgc
     3241 ccgctgcccg ccgacctgcc caccgcgtcc ggttctgcca ctccgtcaaa cggccccgac
     3301 tggaacctga ccatgatcca tgccgatcag gtctgggatc aactgggcgt gcgtggagat
     3361 ggcatcatca ttggtcaatc tgactccggc gtccagggcg accatcccga actgaatgac
     3421 tcttacctcg ggcgcgatgg tcaaaacgat taccgctggt atgatccctg gtttggctcc
     3481 acctcgccga ccgatatcgg cgggcatggc acccataccc tgggcagcat cctgggcaat
     3541 caggttggcg tggcccccga tgctgattgg atcggctgtg tcaatttagg gcgtaatctg
     3601 ggcaacccgg cgctttacct ggactgctgg cagtttatgc tggccccctt cccccagact
     3661 ggcgatccgc tgaccgatgg agaccccgcc cgcggggcca atatcctcaa caattcctgg
     3721 ggctgcccgg agattgaagg ctgcgacccg tcaaccttta tatcagccac caaagccctg
     3781 cgttcagcgg gcatctttgt cgtcgtctct gctggcaaca gcggctacgg cggctgcggc
     3841 tcagtcaaag acccgcccgc cacagatgaa tttgtctatt cggtcggcgc gattgactcc
     3901 aacggcagtc tggcagagtt cagcagcatc ggccccgtgg ttgtggacgg cagcgagcgc
     3961 accaagcccg atatcgtcgc cccgggggat ggcgtgccct cctcctatcc cggcagcagc
     4021 tacgaggttg ccagcggtac ctccatggct gggccgcatg tggtcggcgt agtcgccctc
     4081 atgtggtctg ccaaccccga cctaatcggt gaggtggagg aaaccattgc gattctcaac
     4141 gagacggccc aaccctacga aggcccatac cctgaatgtg tgactgaccg cagcgccccc
     4201 aacaacgcgg tggggtatgg catcgtggat gcctatgccg ctgtgctgcg cgccctccag
     4261 gttcatccct gattggagca cctgatgagc gcaacccacc gcccctctgt gggtttaatc
     4321 caatagacca tcactatgag cctggatgcg cctgaactca ctgatgccga agccctgacg
     4381 cgcgccgctg atggtgatct ggacgcgttt ggtgtgctgt atgagcgcta cgtcacgcgg
     4441 atttacaatt acatctatta ccgcacgggt aacccacacg aggctgaaga tctgacggct
     4501 cgcgtttttc agcgggccgc tcgccacatc cccaactatc gtcaaatggg cgtccctttt
     4561 gcggcttggc tctatcgcat tgcgcataac cttgtcgcca actggcaccg cgatcgcagc
     4621 cgccgccgtg aagtccccct ggacgatcag ccccgcctga tgatgccgat tgaacatccc
     4681 gaatcaacca ttgtgcgcac ccaggagatg caaaccctgc ttaaagctat ccgctcattg
     4741 acccccgaac gccaaaacct ggtgatcttt aaattcgttg agcagctttc caacgccgaa
     4801 attgccgtca tcatggggcg gaccgaaggc gccatcaaga gcctttacca ccgtaccctg
     4861 ttggccctgc gtgaagagtt ggaacgcctc gagaagtcgg gaggttgaga tgttgcgtat
     4921 tgtgaccgat ggcgccgtag attgtcctgc cgaatggcgg gatgaatatg gcattgatgt
     4981 tctgccgctc tacatccaga ttggcgatca aaccctggtc gagggcgtca acgtcagtgt
     5041 tgataacttt tatggtctgg tggagaagag ccagaaaccc cccaagacat cgctgcctgc
     5101 tcccgcccag atcgtcgcat tttatcacca gattgcccgc cctggcgaca gcatcctgtc
     5161 cattcacatc gccagcaaaa tgtcgggcac ttttgccgca gtccaactgg ctgcccgaga
     5221 ggttgcctcc gaattttcca ttcacgtctt cgattctggc gctggctcgg cagctttagg
     5281 ctttatgtgc cgtgaagctc gccgcctgga acgcgccggg gcatctattc aaaaaacctt
     5341 gcagcgcctg gagcaggccc gcca
//
LOCUS       DAOPVS010000128         5363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15326_length_5363_cov_50.418237, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000128 DAOPVS010000000
VERSION     DAOPVS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15326_length_5363_cov_50.418237"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1510)
                     /locus_tag="PKL11_09025"
     CDS             complement(56..1510)
                     /locus_tag="PKL11_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNZ13783.1"
                     /translation="MSPKLSYLQTLIPRPLSLHPGEGQFTFSPSTQIYYADESIAAEA
                     AYLAEWLGCSLPPQPVSGEIPPGAILLIQTESPVLGSEGYELSITPDQVRLRAHQPAG
                     IFWAIQTLRQLAPAEPGTIHWPACEIQDLPRYPWRGMMLDVARHFFPVATIKSVIDRL
                     ALYKLNRLHLHLTDDQGWRLMIHSWPRLAEIGGLAAVGGDPGGYYTQSDYAEIVAYAQ
                     ARHIMIIPEVDLPGHTNAALSAYAELNRDGQAAAPYTGTRVGFSSLCTDREHTYRFLM
                     DVLGEIAAITPGPYLHIGGDEAAATPRPDYLRFIPRLQHIVTSLNKQMIGWEEISRAT
                     LKPGAVAQAWRSSLVTRAARQGARVILSPARKIYLDMKYNPLSPLGLAWAGFVEVRQS
                     YNWNPAYAVRNLPEDTILGVEAPLWTETVRSLADLDWLVFPRLIGVAEIGWTPQSLRL
                     WSDYRARLAAHAPRLVQQSINFYRSPQVPWVLSS"
     gene            complement(1510..3339)
                     /locus_tag="PKL11_09030"
     CDS             complement(1510..3339)
                     /locus_tag="PKL11_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13784.1"
                     /translation="MGFGIGISSPGMGPRGALDSFATETDERGKVFNRRVVVRLLTYL
                     RPYAGKMALAFVAMLFVTAFTLLNPYLLKVIVDQYIGAGNQTGLLTASLMMVGSYVGL
                     YLSNASQQYLLTWVSQKVLSNLRSELFRHLQRLSLSYHDTHIVGVTVSRVMNDVATIN
                     ELLSQGLITLMGDTLILGGIIVIMLTMNPRLALLTFTVLPLMLLATIWFSRRARVAFR
                     ETRTRVARVVGDLAEDISGVRVIQAFGQENTSQERFNQVNEANRDANISAMNLSFIFL
                     PSIEFLGVLATAIVLWFGGRMVGEQVTLGVLVAFLSYVTRFFQPIQELSRMYTTMQAA
                     MAGGEQVLKLLDTPPEVQDSPDAVEMPPVQGRITLEQVSFAYRPDLPEVLHAIDLTIE
                     PGQTVALVGPTGAGKTSIGNLIARFYDVSGGAVKIDGVDVRTVTQESLHQQIGLVPQD
                     SFLFSGTIADNIRFGRPAASDAEVEEAARLANAHAFITSRPDGYETRVLEGASNLSVG
                     QRQLLCIARAILTDPRILILDEATSNVDSLTETLIQDALRKLLKGRSAIVIAHRLSTI
                     RSADVICVVQDGRIVEQGPHAELLARGGAYAALYQRQFEAESL"
     gene            complement(3342..5129)
                     /locus_tag="PKL11_09035"
     CDS             complement(3342..5129)
                     /locus_tag="PKL11_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13785.1"
                     /translation="MKPPSKPASQQTRTLIRCFGYLRPHWKLTTGAYLTMLVIDILNM
                     VNPQIIRWTIDNGIGGDLVEPRRSTLLGLAVGALLLLVIIKGVLTYFQGMWTEVASQG
                     VAYDLRNAIQRKITELSFSFHDQAEAGDLLSRAIQDVERIRFLTGRASFRVIEGIVMM
                     LITAGVMISMNPRLGLLGLIAMPLLAVQSIRFGRVFRPLSMQIQKQLAVLTTRVEQNL
                     RGARVIKTFSQQTSEIDRFEVENQRWLDLSSTSARLQSINMPLLGLIANVSSVFILWY
                     GGTMVIQGQLTLGELVAFTTYMGQLVTPVRFLGMILPAISMAEASAERVFEILDTVPE
                     VSEVADAQPLKLERGHVSFENVSFAYARRGDVLKNISFEARPNQVVALLGLTGSGKTT
                     VVNLIPRFYDPTEGRIIMDGQDIRNVTIQSLRSQIGMVLQETTLFAASIRENIVFGRP
                     DATQAEIESAARAAQAHDFIMRSANGYDTEVGERGITLSGGQKQRLAIARALLTDPRI
                     LILDDATSSVDTETEHLIQLALETVMRGRTTFVIAHRLSTVQNADLILVLDKGRIAAQ
                     GRHDDLLRTSPLYASIYQQQLKPRAEGRA"
BASE COUNT         1230 a         1385 c         1737 g         1011 t
ORIGIN      
        1 attgctggtg aactggatcg aatcactcat agcaggctcc tttgcttcta ttatatcagg
       61 atgagagtac ccagggtacc tgcggagaac gataaaaatt gatgctttgc tgaaccaggc
      121 gcggcgcgtg agccgccaac cgggcgcggt agtcgctcca gaggcgcagg gactgcggcg
      181 tccagccgat ctcggccacg ccaatcaggc gggggaagac aagccagtcg aggtcagcca
      241 gactgcggac agtctcggtc cagagcgggg cttcgacgcc caggatggta tcttcgggga
      301 gattgcgaac cgcataagcc ggattccagt tataagactg gcgcacttca acaaaaccag
      361 cccaggccag gcccagcggc gagagcgggt tatatttcat atcgaggtat atttttcggg
      421 caggcgagag gatcacgcgg gcgccctggc gggctgcccg ggtgacgagg ctgctgcgcc
      481 aggcctgggc cacggcgccg ggtttgagtg tggcgcgaga aatttcctcc cagccgatca
      541 tctgtttgtt cagagaggtt actatgtgct gcagccgggg gataaaacgc agataatccg
      601 gacggggggt ggcggcggcc tcatccccac caatgtgcag gtaaggtccg ggcgtgatgg
      661 cggcgatctc gcccagcaca tccatcagga agcgataggt atgctcccgg tcggtgcaaa
      721 gcgaggagaa gccaacccgg gtgccagtat agggagcggc agcctgacca tcgcgattca
      781 gttcggcgta ggcagacagc gcggcgttgg tatgaccggg taaatccacc tcggggataa
      841 tcataatatg gcgggcttga gcataggcaa cgatttcagc atagtctgac tgggtgtagt
      901 agccgccggg gtcgccgcca actgctgcaa gaccgccaat ttcagccagg cgcggccagg
      961 agtggatcat cagccgccag ccctgatcat cggtcaggtg aaggtgcagc cggttgagct
     1021 tgtagagggc aaggcggtca atcaccgatt taattgtggc tacggggaag aaatgacggg
     1081 ccacgtccag catcatgccg cgccagggat agcgcggcag gtcctgaatc tcacaggcgg
     1141 gccaatgaat tgtgccgggc tcagccggcg ccagttggcg cagggtctga atggcccaga
     1201 agatgccggc aggctgatgg gcgcgcaggc ggacctgatc gggcgtgatg gaaagctcat
     1261 acccttcaga acccaggaca ggggactctg tctgtataag cagaattgca ccgggtggga
     1321 tttcaccgga gactggctgt gggggtaaag agcagccaag ccactctgcc agataggcgg
     1381 cttcggcggc aatcgattcg tctgcgtagt aaatttgggt tgaaggggaa aatgtgaatt
     1441 ggccctcgcc tggatgcaat gaaagcgggc gagggatgag ggtttgtaag taagaaagtt
     1501 tgggtgacat tagagcgact cagcttcaaa ctggcgctgg tagagcgcgg cataagcacc
     1561 gccgcgtgcc agcagttcgg catgggggcc ctgctccaca atacggccat cttgcaccac
     1621 acaaatcaca tcggcagagc gaatggtgct gaggcggtgg gcaatgacga tggcgctgcg
     1681 gcctttgagc agtttgcgca aggcatcctg aatcagggtc tcggtcaggc tgtccacgtt
     1741 ggaagtggct tcgtccagga tcaggatgcg gggatcggtg agaatggcgc gggcaatgca
     1801 caaaagctgg cgctgcccaa ccgagaggtt tgatgctccc tctaagaccc gggtttcata
     1861 tccatcgggg cgggaggtga tgaaagcatg ggcatttgcc agacgggcag cctcctcaac
     1921 ctcggcgtct gaggcagcgg ggcggccaaa gcggatatta tcggcaatcg ttccagagaa
     1981 aaggaaggaa tcctgcggga ccagaccaat ctgctgatgc agggattcct gagttaccgt
     2041 gcgcacatcc acgccatcaa ttttaacggc tccgccgctg acatcataaa aacgggcgat
     2101 caggttgcca atggaggtct taccggcgcc ggtggggcca accagtgcca cggtctggcc
     2161 cggctcaatg gtcaggtcaa tggcatgtaa cacttcgggg agatcggggc gataggcaaa
     2221 cgagacctgc tccagcgtaa tgcggccctg taccgggggc atttccacgg catcggggct
     2281 gtcctgaacc tcgggaggcg tatccagcag tttgagcacc tgttcgccgc ctgccatggc
     2341 cgcctgcatg gtggtgtaca tgcggctgag ttcttgaatg ggctggaaga agcgcgtgac
     2401 ataggacagg aaagccacca gaacacccag tgtgacctgc tcaccgacca tgcggccgcc
     2461 aaaccagagc acaatggcgg ttgccagcac acccaggaat tcgatgctcg gcaggaagat
     2521 gaacgacagg ttcatggcgc tgatgttggc gtcacgattg gcctcattga cctgattgaa
     2581 gcgctcttgc gaggtgttct cctgcccaaa ggcctgaatg acgcgcacac cggagatgtc
     2641 ttcggccagg tcgccaacca cgcgggccac gcgggtacgg gtctcgcgga aggcaacgcg
     2701 cgcccggcgc gagaaccaga tggttgccag aagcatgagc ggcaagaccg tgaaggtaag
     2761 cagagcaagg cgcgggttca tggtgagcat gatgacgata atgcccccca ggatcagcgt
     2821 gtcgcccatc agggtaatga ggccctgcga gagcagttca ttgatagtgg caacgtcgtt
     2881 catcacgcgg gagacggtga cgccgacaat atgggtatcg tggtagctga gtgacagtcg
     2941 ctgcaaatgc cggaacaatt cgctccgcaa attggagaga actttctggc tgacccaggt
     3001 gagcagatac tgttgggagg cgttggagag ataaagccca acgtaggagc cgaccatcat
     3061 cagggaagcg gtcaacaacc cggtctggtt tccggcgccg atgtattgat caacgataac
     3121 cttgagcaag taagggttga gcaaggtaaa agctgtgaca aagagcatgg ccacaaacgc
     3181 cagcgccatt ttgccagcgt aggggcgcaa ataggtcagc agacggacaa ccacccggcg
     3241 gttgaagacc ttgccgcgct catcagtttc agtggcaaat gagtccagcg cgccgcgcgg
     3301 gcccatgcca ggagaagaaa tgccaatgcc aaagcccatg gttatgccct cccttcggcg
     3361 cggggtttga gctgctgctg gtaaatggaa gcatacagcg gtgaggtgcg cagcaggtca
     3421 tcgtgccgcc cctgggccgc aatccggccc ttatccagca ccagtatcag gtcggcattt
     3481 tgaacggtgc tgaggcggtg tgcgatgacg aaagtggtgc gcccgcgcat gacggtttcg
     3541 agtgcaagct gaatgaggtg ttcggtttcg gtatccacgc tggaggtagc gtcatccagg
     3601 atcagaatgc ggggatcggt gagcaaggcc cgggcaattg ccaggcgctg cttttgccca
     3661 ccggagaggg tgatgccgcg ctcgccgact tcggtatcgt agccattggc cgagcgcatg
     3721 ataaagtcat gcgcctgagc ggcgcgagcg gcggattcaa tctcggcctg agtggcatcg
     3781 gggcgaccaa agacaatatt ctcgcggatt gaggctgcga acagggtggt ttcctgcagc
     3841 accatgccaa tttgactgcg cagggattgg atggtcacgt tacgaatatc ctgaccatcc
     3901 atgataatcc ggccttcagt ggggtcatag aagcggggaa taagattgac cacggtggtc
     3961 ttgccggaac cggtgagacc cagcagggcc accacctggt tggggcgggc ctcaaaggag
     4021 atgtttttca ggacgtcgcc gcggcgggca taggcaaagg agacgttctc aaaagagaca
     4081 tggccgcgct ccagcttcaa cggttgggca tcggccactt cgctgacttc agggacggtg
     4141 tccaaaatct caaaaacgcg ctcggctgag gcttctgcca tactgatggc gggcaggatc
     4201 atgccgagga aacgcacggg agtgaccaac tgccccatat aggtggtgaa ggcaaccagt
     4261 tcgccgaggg ttagctggcc ttgaatgacc atggtgccgc cgtaccagag gatgaacaca
     4321 ctgctgacat tggcgatcag ccctaacaac ggcatgttga tagactgcaa gcgggcagaa
     4381 gtggaagaca gatcgagcca gcgttggttt tcgacctcaa accgatcaat ttcagaggtc
     4441 tgctgcgaga aggttttgat cacgcgggcg ccgcgcagat tctgttcaac acgggtggtg
     4501 aggacggcca actgcttctg gatctgcatg gacagcggac gaaatacccg cccaaagcga
     4561 atggattgaa ccgccagcag cggcatggct atcaggccca gcaggcccag gcgagggttc
     4621 atactgatca tgacacctgc ggtaatgagc atcatcacga tgccttcaat cacgcggaaa
     4681 gaggcccgcc cggtgaggaa gcggatgcgc tctacgtcct ggatggcgcg ggagaggagg
     4741 tcgccggctt cagcctggtc atgaaaggag aaggacagtt cggtaatttt gcgctggatg
     4801 gcattgcgta agtcgtaagc aacaccttgc gaggcaacct cggtccacat accttgaaaa
     4861 taggtcagga cgcctttaat gatcaccagc agaagcaggg cgcccaccgc caggcccagc
     4921 agcgtgctgc ggcgcggctc aaccagatcg ccgccaatgc cattatcaat ggtccagcgg
     4981 atgatctggg ggttgaccat attcagtatg tcaatcacca gcatggtcag ataggcgccg
     5041 gtggttaact tccagtgtgg acggagatat ccaaaacaac gtatgagggt gcgggtttgt
     5101 tgggacgcgg gttttgaagg gggtttcatc aggtgcgggt cactttataa aggtaaatgt
     5161 cagacaattt aaccggatgc aacagaggta ttatactcca ggcatttggg attcaaagat
     5221 taggaatatc atagagtctt ctggtatcag ggctgagaaa catctatgcc gcctgagggc
     5281 aattatgcag cctgggctga ataaaaaacc cgccgccggt tctgccaggc ggcgggttta
     5341 tggggttatg gaagcgaatt agc
//
LOCUS       DAOPVS010000129         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15350_length_5354_cov_56.033403, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000129 DAOPVS010000000
VERSION     DAOPVS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15350_length_5354_cov_56.033403"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..946)
                     /locus_tag="PKL11_09040"
     CDS             complement(23..946)
                     /locus_tag="PKL11_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNZ13786.1"
                     /translation="MSNAQPRLTPWQRFRKELAAEKLSWRTVQDYGLILIGALVQALA
                     MRLFLVPAQLVSGGISGAAQIINHFVTWPIGLMVFIGNVPMFILGWRYLGGARFALRT
                     ALSVTAFAIFTDLLVIFIPAQGVTDDLFLDCLYGGLLLGVGLGIVYRGRGTSGGSDIL
                     GRILNHRLGISISQAYLITDTIVVLAGGFAFDWEKALYGMIVIYVSGLAAEMISEGSA
                     IFRTAIVITDNPRAVSQRIMESLERGVTLLSGTGAYTGTEREVLYCVITRSEVNQMKE
                     LVREVDANAFIVIGQAHEVLGEGFRSLIRVE"
     gene            1115..>1671
                     /gene="coaE"
                     /locus_tag="PKL11_09045"
     CDS             1115..>1671
                     /gene="coaE"
                     /locus_tag="PKL11_09045"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNZ13787.1"
                     /translation="MSKWPGKYVIGLTGNIGTGKSVVRRMLEHLGAYGIDADALVHRA
                     IAKGAPGYQAVVKAFGTWILGPDEQIDRNRLGRLVFSEPEALVTLEKIVHPLVAQAVD
                     FIVQHATQPVIVIEAIKLLESGMAKSCDKVMVTYAPPETQIARLTQNRHMTEAEARQR
                     ITAQPPQEQKMAAAGVVIRNIGTFE"
     assembly_gap    1672..1771
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1772..2065
                     /locus_tag="PKL11_09050"
     CDS             <1772..2065
                     /locus_tag="PKL11_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13788.1"
                     /translation="RTTDIYIDPSIPVAQALPVLVNEMERASKDLQCEASLIFATPDL
                     AQDESLWKGLGYERRTPDTLGVLAWQEAAQESMPANTVLLFKKLRQDRVLRPI"
     gene            2139..3023
                     /gene="cdaA"
                     /locus_tag="PKL11_09055"
     CDS             2139..3023
                     /gene="cdaA"
                     /locus_tag="PKL11_09055"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNZ13789.1"
                     /translation="MAELLSEILFVFQRMTWLSVLDLVLVTLVLFVILLLLRDTQAMA
                     LLRGVLLLVILLALLTTVVELPAFSWLIKTTLPALLLAVPVIFAPEIRRGLERLGRAG
                     AGALLPYQRRGNLEALQQTVRTIVSAAARLSARQHGALIILQRADSLREYMQTGITLN
                     ARVTPELLLQVFYPNTPLHDGAVIISDNMLVAASCVMPLSASGVLNRAADRRMGLRHR
                     AALGISEVSDAIAVVVSEETGSISIAQGGKLMRRLDIERLENIILAFYQSPSPAVRPA
                     HFFSRIFQGDDPNRPPEN"
     assembly_gap    3111..3210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3211..4155
                     /locus_tag="PKL11_09060"
     CDS             <3211..4155
                     /locus_tag="PKL11_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNZ13790.1"
                     /translation="VQIGARPAQIESYTPATVEITLENLMTRTLPVNLAIQGEPAVGY
                     QAETPALSNDSVTITGPEALVNQVDQVQAVLDVNLLQQSIVRTIPLKAVDATQSPVTG
                     ITLSPSEITVTQEITQRFGYRSVVVNVVVSGQVAQGYRLTNISVFPPAVTVFSADPSI
                     VDSLPGYVQTEALDLTNATDDLDMFLDLSLPDGLSVVGGQETVLVRVGIAAIESSAAY
                     SNMTVEVVGLSSTLKATIAPEQVTVIVAGPLPMLDQLTIDGIRVMVDVTGLTAGTYQL
                     TPNVEIAIQNLRVESILPETVEVVISSALSPTPTPRKR"
     gene            4167..>5354
                     /gene="der"
                     /locus_tag="PKL11_09065"
     CDS             4167..>5354
                     /gene="der"
                     /locus_tag="PKL11_09065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNZ13791.1"
                     /translation="MRKPVVALVGRPNVGKSTLFNRLAGEPLAIVDDTPGTTRDRLLS
                     ESEWNGIPFHIVDTGGIDPSTGGKKPLSIGSADFIKEIRTQAQVAIEEADVVLFITDA
                     ISGVTPADQEVAQILRRSQKVKDGEPFPPVLLVVNKADSSNLRSGAPEFYELGMGEPY
                     PISAVHGTGTGDLLDALVAAFPEQEEEEEDNSVKIALVGKPNVGKSSLLNRLSGAERA
                     IVSDIPGTTRDAIDTRMEFNDLPVTLIDTAGIRRRGKVEPGVEKYSVIRSMRAIERAD
                     VALLMIDAQQGLSAQDAHIAGFILEAWKSAVVLVNKWDVVEKDNDTMNQFTAKVRKDL
                     NFMDYVPILFISAKTGQRVDQVLPLALRVQEERLARVTTGSLNRILQAAQDLHSPSSR
                     TGQH"
BASE COUNT         1152 a         1339 c         1612 g         1051 t
ORIGIN      
        1 ccatccaact gccgttctag gctcactcga cacggatgag ggaacggaag ccctcgccca
       61 atacctcgtg tgcctggccg atgacgatga aggcattggc atccacttcc cggacgagct
      121 ccttcatttg attgacttct gaacgggtaa tcacgcagta caggacttcg cgctctgttc
      181 cggtgtaggc gcctgtgcca gaaagcagcg tgacgccgcg ttcaagcgat tccatgatgc
      241 gttgagaaac agcgcggggg ttgtcggtaa tgacaatggc tgtgcggaag atggcggagc
      301 cttcagagat catttcggca gccagaccgc tgacataaat gacgatcatg ccatagaggg
      361 ctttctccca gtcaaaggca aagccgcccg ccaaaacaac gatggtatcg gtaatcagat
      421 aggcctgtga aatggaaatg ccgaggcggt gattgagaat gcgcccgagg atatctgacc
      481 cgccgctggt gccgcggccg cgatagacaa tgcccaaccc cacccccaag agcagaccgc
      541 catacagaca gtccagaaag agatcatccg tgacgccctg ggccgggatg aagataacca
      601 ggagatcggt aaaaattgca aaggcagtga cggacagggc ggtgcgcagg gcgaagcgcg
      661 ctccacccag gtagcgccac cccaggatga acatgggcac gttgccgatg aagaccatca
      721 gaccgatggg ccaggtgaca aagtgattga taatttgggc ggcaccgctg atgccgccgc
      781 tgactaactg ggcagggacc aaaaagaggc gcatggcaag cgcctgcacc agcgcgccaa
      841 tcaggatcag gccgtaatct tgaacggttc gccaggataa cttttcggca gccagctctt
      901 tgcgaaaccg ctgccagggt gtgagccggg gctgggcgtt tgacatgtgg ggacctcctg
      961 agatcatcac gggtaagact gagcggtatt ataccgcacg ggggaatgga cgaggcggag
     1021 attaatgaca gggctggaat tgccttaaac aaatgaatgg tataataatg atgttatctg
     1081 tgtgaaatta tctcttcccg ccagaggtgg agcaatgagt aaatggcccg gcaaatacgt
     1141 tatcggtctc accggcaata tcggcaccgg caagagtgtg gttcggcgca tgctggaaca
     1201 cctgggggct tatggtattg atgcggacgc gctggtgcat cgtgcaattg ccaagggtgc
     1261 gccggggtat caggcggtgg tcaaggcgtt tggcacctgg attctgggtc cggatgagca
     1321 gattgaccgc aaccggctgg gaagactggt gttcagcgag cctgaggcgt tagttaccct
     1381 ggaaaagatt gtccacccgc tggtagcgca ggcggttgat tttattgttc agcatgccac
     1441 gcaaccggtg attgtaattg aggcgatcaa attacttgaa agcgggatgg cgaaatcgtg
     1501 cgacaaggtg atggtgactt atgccccacc ggaaacacag attgcccgcc tgacccaaaa
     1561 tcggcatatg accgaggcag aagcccggca gcgcattacg gcccaaccgc cccaggagca
     1621 gaagatggcg gcggcgggtg tggttattcg caacattggc acgtttgaag annnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcacgact gatatttata ttgaccccag
     1801 tatcccggtg gcgcaggcgc tgcccgtgct ggtgaacgaa atggagcgcg cctcaaaaga
     1861 tctacaatgc gaggcttcgc tcatttttgc aaccccagac ctggcacagg atgaatcgct
     1921 gtggaaggga ttggggtatg aacgccgcac gcccgacacg ctgggcgttc tggcctggca
     1981 ggaagccgct caggaatcca tgccagccaa tacggtgctg cttttcaaga agctgcgcca
     2041 ggatcgagtc ctgcgcccca tctaaacggc tttgcgccga tcaattgagg ggggcagcag
     2101 gcgagcggtt ataatcgtac aaaccctgtg gtaccgccgt ggctgaatta ttaagcgaaa
     2161 tcttatttgt attccaacgg atgacctggt tgagcgtgct tgacctggtt ctggtcacgc
     2221 tggtgctgtt tgtcattttg cttttattgc gcgacacgca agccatggca ttgctgcggg
     2281 gtgttttgct gctggttatt ttgctggcgc tgctgacaac agtggtggaa ctgccggcgt
     2341 tttcgtggct gatcaaaacc acactgccgg ccttgctgct ggcggtgccg gtgatctttg
     2401 cgccagagat tcgccgggga ctggagcgac tgggacgcgc aggggcgggc gcgctgctgc
     2461 cctatcaacg gcggggcaac ctggaagctc tgcagcagac ggtgcggacg attgtcagcg
     2521 ccgcggcgcg gctttcggcc cggcagcatg gagcgctgat tattctgcaa cgcgcagaca
     2581 gcctgcggga atatatgcaa actggcatta cgctgaacgc gcgggtaacg cccgaacttc
     2641 tcctgcaggt tttttacccc aacacaccgc tgcacgatgg cgcagtgatt atctccgata
     2701 atatgctggt agcagcatcc tgcgtgatgc cgctgtcggc gagcggggtg ctgaaccggg
     2761 cggcagaccg gcggatgggc ctgcggcacc gggccgcgct gggtatttca gaggttagcg
     2821 acgcgattgc ggtggtggtc tctgaagaaa ccggctccat ttccattgcc cagggcggaa
     2881 aattgatgcg gcggctggat attgagcggc tggaaaatat tatcctggcg ttctaccagt
     2941 cgccctcacc ggcggtgcgc ccggcgcatt ttttcagccg gatattccag ggggatgacc
     3001 ccaatcggcc accggagaat tagcatgatt gattttctgc gccgcgcaat caaaaccctg
     3061 ccgaccctgc tgaccgcctt tctgctggcg ctggtgttgt ggatttatgc nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgcaaattg gcgcccgccc ggctcagatt
     3241 gagagctaca ccccggcaac ggttgaaata acgctggaaa acctgatgac gcggacgctg
     3301 cccgttaatc tggccatcca gggagaacct gcggtggggt atcaggctga aacaccagcc
     3361 ctgagcaacg acagtgtgac cattacaggg cccgaagccc tggtcaatca ggttgatcag
     3421 gtgcaggcgg tgctggacgt caatttattg cagcaaagca ttgtgcgaac aatccccctg
     3481 aaggcggtgg atgccacaca atcccccgtt acagggatca ccctcagccc cagcgagatt
     3541 acggtaaccc aagagattac gcaacgattt ggctatcgca gtgtggttgt gaatgtggtg
     3601 gtcagcgggc aggtagccca gggataccgg ctgaccaata tttcggtctt tccgccggcg
     3661 gtcacggtgt tctcggcaga cccgagcatt gtggacagcc tgcccggcta tgtgcaaact
     3721 gaggcattag acctgaccaa tgcgacggat gacctggata tgttcctgga tttgagcctg
     3781 ccggacgggt tatcggtggt gggcggacag gaaacggtgc tggtgcgggt gggcattgcc
     3841 gccattgaaa gcagcgccgc ttattccaat atgacggttg aagtggttgg attgtcgtca
     3901 acgctcaagg cgaccatcgc ccccgagcag gtgactgtga ttgtagccgg accgctgccc
     3961 atgctggatc aactgacgat tgatgggatc cgcgtgatgg ttgatgtgac cggattgaca
     4021 gctggaactt accaattaac ccccaatgtg gaaatcgcga ttcagaattt gcgggtagaa
     4081 tcgatacttc cagagaccgt ggaagtggtg atcagcagtg cgctgagccc cacccccaca
     4141 ccccgcaaac gataaggagt tttgtgatgc gtaaacctgt ggtagccctg gttgggcggc
     4201 cgaatgttgg caaaagcacg ttatttaacc gcctggcagg ggagccgctg gcaattgttg
     4261 atgatacccc cggaacaacc cgggaccggc tgctttccga gtcggagtgg aacggcatac
     4321 ccttccatat tgtggatacg ggtgggattg accccagcac gggcggcaaa aagccgctct
     4381 cgattggttc ggcggatttc atcaaggaaa tccgcaccca ggcccaggtt gccattgagg
     4441 aagcggatgt ggttctgttt atcacagatg ccatcagcgg ggtcacacct gcggaccagg
     4501 aggtggcgca gatcctgcgc cgcagccaga aggtgaaaga cggcgaacct ttcccgccgg
     4561 tgctgctggt ggtcaacaaa gcggatagtt ctaatctccg cagcggcgct cctgagtttt
     4621 atgaactggg catgggagag ccttacccga tctcggcggt gcatgggacg ggcacaggcg
     4681 acctgctgga cgcgctggtg gcggcatttc cggagcagga ggaagaggaa gaagataact
     4741 cggtcaaaat cgcactggtc ggcaagccga atgttggaaa atccagtttg ctcaaccggc
     4801 tgtcgggcgc agagcgcgcc attgtcagtg atattccggg aacaacacgc gacgcgattg
     4861 atacccggat ggagtttaat gatctgccag tcacgctgat tgatacggcg ggcatccggc
     4921 gacgcggcaa ggtggaaccc ggcgtggaaa agtacagtgt cattcgctca atgcgggcta
     4981 ttgaacgcgc agacgtggcc ctgttgatga ttgatgccca gcaagggctc agcgcgcagg
     5041 acgcgcatat tgccgggttc attctggaag cctggaaaag cgcagtggta ctggttaata
     5101 aatgggatgt agtggaaaaa gataacgaca cgatgaatca gttcactgca aaagtgcgta
     5161 aagatctgaa tttcatggat tacgtgccga ttttattcat atctgcaaaa accgggcagc
     5221 gcgtggatca ggtgctgccg ctggcgctgc gggtgcagga ggaacgtctg gcgcgggtga
     5281 cgacgggcag cctgaatcgc attttgcagg cggcacaaga cctgcactcg ccatcttcac
     5341 ggacggggca gcac
//
LOCUS       DAOPVS010000130         5324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15453_length_5324_cov_48.747390, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000130 DAOPVS010000000
VERSION     DAOPVS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15453_length_5324_cov_48.747390"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..429)
                     /locus_tag="PKL11_09070"
     CDS             complement(118..429)
                     /locus_tag="PKL11_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNZ13792.1"
                     /translation="MSSKDEFGAFVIGFVVGGLTGSVLALLFAPQSGEETRTVIRERA
                     IELSDKASSTATEVYSKAEDAANEAVHRAEALLNEAKTKAKEIASRKKADAPAEEIPA
                     A"
     gene            complement(477..962)
                     /locus_tag="PKL11_09075"
     CDS             complement(477..962)
                     /locus_tag="PKL11_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13793.1"
                     /translation="MTDTIETPNTPAPAADIKAEAPTPVPLTPEELAAQKQMRTRLII
                     LAIVLVVILGLIVVAVLALVNAGVGTTAMVRDIFIIFMALMMILLSGALVVLIIQLAI
                     LINLLQNEIKPILHSTNETVNTLKGTTVFLSNNLVQPVLKLNEYLAGLKKLLDLLKIG
                     R"
     gene            complement(1052..1894)
                     /locus_tag="PKL11_09080"
     CDS             complement(1052..1894)
                     /locus_tag="PKL11_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13794.1"
                     /translation="MKPKSKLLLFLFCALLLPISLAAAPGMGDGIAQAEKAGNTIYLP
                     IIMRADPNPPSDLVVFEAFLSPYCGYCQAAAPIIDNQLVPEYAGQPVLFLEHSALEYT
                     PRQGRWWDGWAVGGSVGFPMVMTDSGNQVRERLTSGTDFYNLYKAMVDTALANPQINA
                     DIQTTVNRSNDVVNFTINVTNQGSLTLGTANKATLWVLVYEQSESGPVSDGLTQRYVR
                     AQVSQALSANLAPGASAQYLLTVNSLSGVDWSKMHAVVILDYRPNSSLRPYNTYQAKM
                     VEIP"
     gene            2072..2698
                     /locus_tag="PKL11_09085"
     CDS             2072..2698
                     /locus_tag="PKL11_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13795.1"
                     /translation="MHTLRFSLAFLVLLLSLFGLAFQSPVGPVITAPLPGAALQGLIS
                     ITGTTALEGFSSSEVSYGYESDQTGTWFLLQQSRAPVTDGVLATWDTTTISDGNYRLR
                     LQVILTDGRVEEVILRGLRVRNYSIIETSTPTGVVETQVTLAVEPTATRTPAPTISRA
                     TPTPLPDNPAEVTPAGLVYSLLRGANLALIIFAALGLYLLLRRWIRRR"
     gene            2704..3351
                     /gene="pth"
                     /locus_tag="PKL11_09090"
     CDS             2704..3351
                     /gene="pth"
                     /locus_tag="PKL11_09090"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ13796.1"
                     /translation="MESLFEKLGLARQQKLDEDTTPAGVYLIAGLGNPGREYRENRHN
                     AGFMAVDRLAEAWDIHLTRVQSRALIGTGTVLGRRVVLAKPQTFMNLSGEAVSALLRF
                     YKTPVSNLLVFHDDIDLPLGMLRLRPGGGAAGQKGIQSIITRLATPDFARARIGIGRP
                     AGQRGAASYVLQDFSKDEMDILRLVLDRCLSAARTFIESGLEPAMNQYNGDIHER"
     gene            3341..>5324
                     /locus_tag="PKL11_09095"
     CDS             3341..>5324
                     /locus_tag="PKL11_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNZ13797.1"
                     /translation="MNADASQILAAFRSLPVYVDLVADLCAGTLPIPLGLLRAARLPL
                     AAAFHADLQRPILLLSDRMDRALTVVDELGFWTDAPRLLFPEPNPLFYEPAAWGTNTR
                     RDRLSTLTMLARYHIPGAGDPTIPPIIVTTIRALMTRTLPRRDFLKASRILRLGQSVP
                     PESLQRAWVDLGYQPAEVVVEPGQFSRRGGLLDFWPPSEPLPVRLEFFGDEIDTLRRF
                     DPASQRKVESIQQILITPAREVLPGRAAEKGLPVETLTEFDLPRVHISPASLLDYLPR
                     ESLVLVGDLDLLQSLALELEEQAVRLRAESISEGTLPADFPVPYLNWSELADALSGHP
                     WLAVGHSGTGEPSALAQAFSPGPRYGGRLKPLLDDVAVRGRAGESTWIVSRQASRLRE
                     LWAERSTEVGETAIPQILDGTLGEGWLLSLPDAHLNLLTDSEIFGWERPQARQRHRPA
                     AEAPESSYADLRPGDWVVHVDYGIGRYAGLARRMLDGSEREFLCVEYDGGDQLFVPIH
                     QADRLSRYIGPDAAPPTPTRLGTAEWTQSKQRVREEVLRVAQDLLELYAQRQVAVGRA
                     FPSDTPWQQDLEASFPYVETQDQLRAIREVKQDMESPRPMDRLLCGDVGYGKTEVALR
                     AAFKAVMDGRQVAVLVPTTVLAQQHYETFRERLAAFP"
BASE COUNT         1034 a         1622 c         1391 g         1277 t
ORIGIN      
        1 gttctgtcag atctactgca gccagagaat agttcagttc gctaacagcc agaatttttt
       61 ttagaaaata aaagccccga aaccatgctg gttcggggct ttattgagtc gggttactta
      121 cgcagccgga atttcttcag ccggcgcatc agccttcttg cgagaggcga tttccttggc
      181 cttcgtcttg gcttcgttca atagcgcttc agcgcggtgc accgcttcgt tggcggcgtc
      241 ttcggctttg gagtacactt cggtcgccgt gctcgaggcc ttgtcgctca gttcaatcgc
      301 ccgctcgcga atcacagtgc gggtttcttc gccgctctgg ggggcgaaca gcagtgccag
      361 caccgagccg gtcagaccgc ccaccacaaa accgattaca aaagcgccaa attcatcttt
      421 actggacatg gaaaacctcc tatcgaatgg tggaacaaag ggttggtgcc gcccgattac
      481 cgcccaattt tgagcaggtc aagcagcttt ttgagccccg ccagatattc atttagcttg
      541 agaactggct ggaccagatt gttgctcaag aaaacggtgg tgcctttcag cgtgttcacc
      601 gtttcattgg tggagtgtaa gattggctta atctcattct gcagcagatt gatgagaata
      661 gccagttgga taatcagcac aaccagcgct ccgctgagta agatcatcat caaagccata
      721 aagatgatga agatatcgcg caccatcgca gttgtgccta cacctgcatt gaccagtgcc
      781 agcaccgcca caacaatcaa tcccaatatg accacaagca caattgccag gatgatcagg
      841 cgggtgcgca tctgcttttg cgccgccagc tcttccgggg ttaatggcac cggagtgggg
      901 gcttcggcct ttatgtcggc tgccggggca ggggtgttgg gagtttcaat tgtatcggtc
      961 atggtgaata aattatagca taaataagac ctgatgattt gtctactcag gttgtttttt
     1021 atgtaatttc cagcttatcc tgaaggtcag gttaagggat ctcaaccatc tttgcctggt
     1081 aagtgttata aggtcgcagc gatgagttgg gcctgtaatc caggatgact actgcatgca
     1141 ttttgctcca atccacaccg ctcaggctgt taaccgtcag gagatattgg gcgcttgccc
     1201 cgggcgccag gttggcgctc agcgcttgcg agacctgcgc gcggacatac cgctgcgtca
     1261 gcccgtctga aaccggccca ctttcagact gctcataaac cagcacccac agggtggcct
     1321 tattcgccgt ccccagcgtc aggctgcctt gattggttac attgatggtg aaattcacaa
     1381 catcattcga tcggttaacc gttgtttgga tatcggcgtt aatttgaggg tttgccagcg
     1441 ccgtgtcaac cattgcttta tagagattat agaagtctgt cccgctggtc aggcgttccc
     1501 gtacctggtt gccgctgtcg gtcatcacca tcgggaagcc aacactgccg cccaccgccc
     1561 aaccatccca ccagcgcccc tgccttgggg tgtattcaag agcactatgc tccaggaaaa
     1621 gcaccggttg cccggcatat tctggcacca gttgattgtc aataataggc gccgcagctt
     1681 ggcaataacc acaataaggg cttaaaaatg cttcaaaaac aactaaatca gaaggtgggt
     1741 tgggatctgc ccgcataata atcggtaaat agatagtatt cccggctttt tccgcctggg
     1801 cgatgccatc tcccattccg ggcgccgctg ccaggctgat cggcagcagc aatgcacaga
     1861 ataaaaagag cagtaacttg gattttggtt tcattgtttc tcctttttat aggaatacta
     1921 tactgattca aaactcaaat tacaacagga tattttccac gcaatcctcc aaattaactt
     1981 cgtcgggttt gtaaaaggta gcaacgtata ttttcgattt gtttttgcgc cttccatctt
     2041 ttatcgcttc atgctaaact taccgattgc aatgcatacg cttcgttttt ccctcgcctt
     2101 tctggtcctc ttactaagcc tgttcggttt ggcttttcag tcgcctgttg gcccggtgat
     2161 taccgctccg ctgcccggcg ctgccttgca gggcctcatc tcaatcaccg gtacaactgc
     2221 cctggagggt ttctcttcgt ctgaagtatc ctatggctac gaaagtgatc agaccggcac
     2281 ctggtttttg ctgcagcaga gccgcgcccc ggtaaccgac ggcgtcttgg ccacctggga
     2341 taccaccacc atttctgatg gcaactatcg cctgcgcctc caggttattt tgaccgatgg
     2401 ccgggttgaa gaagtgattt tgcgtggact gcgcgtccgt aactattcca tcattgaaac
     2461 cagtacgccc actggcgttg tcgaaaccca ggttaccctg gctgttgagc caacggcgac
     2521 ccgtactccg gcgcccacca tcagccgtgc cacccccacc cccttgccgg acaaccccgc
     2581 ggaggtaacc cctgccggtc tggtgtacag tctgctgcgc ggagccaatc tggctctgat
     2641 tatctttgcc gcactcggcc tctacctttt actgcgtcgt tggattcgga ggcgttaatc
     2701 accatggaat cgttgtttga aaaactgggc ctggcgcgcc agcaaaaact tgatgaagat
     2761 accacccctg ccggcgtcta tctgattgcc ggtctgggca acccgggtcg tgaataccgt
     2821 gaaaatcgcc acaatgccgg tttcatggca gtggaccgcc tggcagaagc ctgggatatt
     2881 catctcaccc gggttcaatc acgcgccctt attggcacgg gcacagtctt gggccgccgg
     2941 gttgtcctgg caaaacccca aacctttatg aacctttcgg gcgaggcggt ttctgcgctt
     3001 ctgcgttttt acaaaacccc tgtcagcaac ctgctggttt tccatgatga tattgacctg
     3061 ccgcttggaa tgctccgcct ccgcccgggc ggcggcgctg ccgggcaaaa aggcattcag
     3121 tctatcatca cccgcctcgc cacccccgat tttgcccgtg cccgcattgg cattggccgc
     3181 cctgccgggc agcgcggcgc agcctcttat gtcttgcaag atttttccaa agacgaaatg
     3241 gatatcctgc gactcgtgct ggatcgctgt ctttcggcag cgcgcacatt cattgaatct
     3301 ggtctcgagc cggcgatgaa tcagtacaat ggcgatatcc atgaacgctg acgcctccca
     3361 gatcctcgct gcttttcgct ccttgccagt ctatgttgac ctcgtggccg atttgtgcgc
     3421 gggcacgctg cccatcccgc tgggcttgct gcgcgcggcc cgccttccac tggcagcagc
     3481 ctttcacgcc gatttgcaac gccccatctt gctcctgtcc gaccggatgg atcgcgccct
     3541 gacggttgta gatgagctgg gcttctggac agatgccccc cgcctgcttt tccccgagcc
     3601 caacccgctg ttttatgagc ccgcagcctg gggcaccaac acccgccgag accgcctgag
     3661 tacgctgacc atgctggcgc gctatcacat acccggcgcc ggtgacccaa ccatcccacc
     3721 catcatcgtc actaccatcc gcgcactgat gacccgcacc cttccccggc gcgattttct
     3781 caaagccagc cgcatcctgc gcctcggtca gtctgtccca cccgaatcgc tgcagcgcgc
     3841 ctgggttgat ctgggctacc agccggccga ggttgtggtc gagcctggtc agttctcccg
     3901 ccgcggtggt ctcctcgatt tttggcctcc ttcagaaccc ctccctgtcc ggcttgaatt
     3961 ttttggcgac gaaattgata ccctgcgccg ctttgacccg gcctcccagc gcaaagtcga
     4021 atccattcag caaatcctaa tcacccccgc ccgtgaggtt ttacccggcc gcgcggctga
     4081 aaaaggcctg cccgttgaaa ccctcaccga atttgacctc ccgcgggttc acatctcccc
     4141 ggccagcctg cttgattatc tcccccgtga gtcactggtc ctggtcggtg atctcgactt
     4201 gctgcaatcc ctggcgctgg aactggaaga acaggcagtc cgcctgcgcg ctgaaagcat
     4261 ctccgaaggc actctgccag ccgattttcc agtcccctac ctcaattggt ccgaacttgc
     4321 cgatgcactc agcggtcatc cctggctggc ggtgggacat tccggcactg gcgaaccttc
     4381 tgcgctggcc caggcctttt cccccggccc gcgctacggc gggcggctca agccgctcct
     4441 ggatgatgtc gcagttcgcg gccgcgccgg cgaatcaacc tggattgtca gccgtcaggc
     4501 ctcccgcctg cgcgaactgt gggcagagcg ctccaccgag gttggcgaaa ccgccatccc
     4561 gcaaattttg gatggcacat taggcgaagg ctggctgctg agccttcccg atgcacacct
     4621 taatctcctg actgacagcg aaatcttcgg ttgggagcgc cctcaggcgc gtcagcgcca
     4681 tcgtcctgcc gccgaggcac ccgagtcgtc ctacgccgac ctgcgccctg gcgattgggt
     4741 agtgcatgtg gattatggca tcgggcgtta tgccggtctg gcgcgccgta tgctggatgg
     4801 ctctgagcgg gaattcctgt gtgtggaata cgatggcggc gaccaattgt ttgtccccat
     4861 tcatcaggct gaccgcctca gccgctacat cggccccgat gctgccccgc caacccccac
     4921 tcgcttgggc accgccgaat ggacacagtc caagcagcgc gttcgtgaag aagtgctccg
     4981 ggttgcgcag gatctcttgg agctttatgc ccagcgtcag gtcgcggtgg ggcgggcttt
     5041 tccatccgat accccctggc agcaagatct cgaagccagc ttcccctatg tcgaaaccca
     5101 ggatcaactt cgcgccatcc gtgaagttaa gcaggatatg gagagcccgc gccccatgga
     5161 tcgcttgttg tgcggcgatg ttggttatgg taaaaccgaa gtcgccctgc gtgcggcctt
     5221 caaagctgtg atggatggac gacaggtggc tgtcctggtt ccgactacgg ttctggccca
     5281 gcagcattat gaaactttcc gtgagcggct ggcagctttt ccgg
//
LOCUS       DAOPVS010000131         5289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15593_length_5289_cov_56.812190, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000131 DAOPVS010000000
VERSION     DAOPVS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15593_length_5289_cov_56.812190"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKL11_09100"
     CDS             complement(<1..377)
                     /locus_tag="PKL11_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13798.1"
                     /translation="MIPPTAWTDPASVTALIDQQLALHPHMQVQDAYKLLYQGVLGSE
                     HLLGIQPGAFEQRLRLEFDALEPDAAESLFEIIHPAGALRRVNLRPYKAQSGDLARLA
                     AACLNAARQPRGTLADLQAVWAL"
     gene            complement(374..1612)
                     /locus_tag="PKL11_09105"
     CDS             complement(374..1612)
                     /locus_tag="PKL11_09105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13799.1"
                     /translation="MHRPGIPALKIEARRLYLIMNAAAALCYTLVFTASNLFVITVAK
                     LEPLQLVLVGTVLELSAFIFEIPTGVVADLFSRRLSVIIGFALVGVGFIVYGGWPYFA
                     TILLAQVLWGLGYTFTSGAAEAWISDEIGEERAAVTYLSAARLDQIASLVAIPLTVLL
                     AARGGLSLPMLIGGGGFILLSVFLWAAMSEDGFTPTPAADRTTWQQMRQTLRQGLGMV
                     RARPALLGILGIGLFYGLYSEAYDRLSTALLLDRFTFPTWLDLPLIGWFGAISAVGML
                     FSLGATALVERLGLTRPRQLALAMGSGTALLVAALAGFAVSHSLAFSIALVWTIGLLR
                     RVNAPLYTAWVNHRLDSQVRATVISMSSQVDALGQIAGGPLLGLAAQALGLQAGLLGS
                     ALLLSPALLLFALQLRGEAR"
     gene            1700..>2101
                     /locus_tag="PKL11_09110"
     CDS             1700..>2101
                     /locus_tag="PKL11_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13800.1"
                     /translation="MKSAERLVKNKFWLAAGTLILIMGMGLPMGIQFAAGQPSEPPQA
                     SGPPPVCLPGEPCLDESGRWVLAEDDSPDGAVLSGFQSTGGPDDYGYTWDDSVTYAWV
                     YATNGTDAGFSGSSPQLTGPIALPFNFTFYGH"
     assembly_gap    2102..2201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2202..4304
                     /locus_tag="PKL11_09115"
     CDS             <2202..4304
                     /locus_tag="PKL11_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13801.1"
                     /translation="PVHLCGSEGIEDADGLDGLAYYPFCYPVSDLKAVRFYKPAALVR
                     TRVYPLYDGQLTQPGQTNTFTFTVGNTGDFGPDTYDLTVASAWAVSLYGPDGITPLDD
                     TDGDTRLDTGAIPPGEVINIIARVTAPDGLSPGAENTASITVRSSLNTTIAKTVQLET
                     TIPAQFAQAYRDQDPGSVNLDLNWAAPQLTLAVTPGEDGHRYEPAVVQTTDQHFVTVW
                     AEWQETGGQQGYRLLSARLNQYGQVIRPPAALTALEPGVDFFSDGSIAAAAAPDGRIG
                     IAWYRLRRIDAGGLSFYNYNIWFAVLNPDGSLAVGPVNLTQQTTWQVDEDDDLHKVYA
                     PTLAVSSDNRFWVSWEQYYRPTGAPLYEIYSTILAADGSVVMPITRMASGIPGSLRYR
                     HPSVLALSGSRFLLAYDYLNYAPFVSSVKYRVYDSSGEGLVSERDTGFYGLQIDAAQL
                     SGGNVLIAADARNHIHYAVINGSSLIPPVIYGTLQHPSDLDESFAVAVAADETDHAIL
                     TWQDGRGQYLYYALVDASGAAVSGPAIYRTAPDSQAWPRLETSATGYSLTTNAWTPPA
                     DVDGLTGFSAGSYGAQAGGAAVVGLHYANQGLTPAASPVLTLTLGDGLSYLSDTSGLS
                     AAVNGSEITWNLPDLDFGAHDQFTVYIGLPAGAPLGTTYPLSLALESEGPEADATNNA
                     DTAEVVAARQMWLPAIQR"
     gene            complement(4318..5196)
                     /locus_tag="PKL11_09120"
     CDS             complement(4318..5196)
                     /locus_tag="PKL11_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13802.1"
                     /translation="MDWTIPLIILQLIVLEGLLSIDNAAVLGAMVTPLPDDRPIDWPP
                     ALRKVGHMLDKVLGNQRTAALRVGLLGAYVGRGVMLLLASLIVNNPWLKIIGALYLMH
                     LAFENLGTVDVETEEGEIRRLKTGSFWSVVVSVELADLVFSLDNVVTAVSLSDKLWVV
                     MLGVAIGIVMMRFAAGLFSRAVQKEPILKTAAYILILNIGIELLLEEFGIFEFADWQR
                     FAISLLTVALCLAYAHIKPLHKLRPAIVWVGQGFGIFSELINWVIVPVTALVRGIIHL
                     IRIIFAPRKPAQPPAA"
BASE COUNT         1046 a         1609 c         1625 g          909 t
ORIGIN      
        1 aacagcgccc agactgcctg caggtcagcc agcgtgccgc ggggctggcg ggcggcgttc
       61 agacaggccg cggccaggcg ggccaggtcg ccggactggg ccttgtaggg ccgcagattc
      121 acgcggcgca gcgcccccgc gggatggatg atctcaaaca gcgactcggc ggcatccggc
      181 tcaagggcgt caaattccag ccgcagccgc tgctcaaagg cgcctggctg aatccccagc
      241 agatgctcag accccagcac cccctggtag agcagcttgt aggcatcctg cacctgcatg
      301 tgagggtgca aggcgagctg ctgatcaatc aaggcagtca cactcgccgg gtcggtccag
      361 gcagtggggg gtatcatcgc gcttctccgc gcaattgcag ggcaaacaac agcagcgccg
      421 gtgaaagcag cagagccgaa cccagcagcc cggcctgcag gcccagggcc tgcgccgcca
      481 gccccagcag cggcccgccg gcaatctggc ccagggcgtc tacctggctg ctcattgaga
      541 tgaccgtggc gcgcacctgt gaatcgagcc ggtgattgac ccaggcggtg tacagcggcg
      601 cgttgacacg ccgcagcagg ccaatcgtcc agaccagggc aatggaaaaa gccaggctgt
      661 gactgaccgc aaaccctgcc agcgcggcca ccagcagcgc cgtaccgctg cccatggcca
      721 gcgccaactg gcgggggcgc gtgagcccca gccgctcgac cagggcggtg gcccccagac
      781 tgaacagcat ccccactgcg ctgatggccc caaaccagcc aatcagcggc aggtccagcc
      841 aggtggggaa ggtgaagcga tccagcagca gcgccgttga gaggcggtcg taagcctcgg
      901 aatacaaccc ataaaacagc ccgatgccca gaatgcccag cagcgccggg cgggcgcgca
      961 ccatacccag cccctggcgc agcgtctggc gcatttgctg ccaggtggtg cggtctgcgg
     1021 cgggggtggg ggtaaagccg tcttcggaca tggcggccca caggaaaacc gacagcagga
     1081 tgaacccgcc gccgccgatc agcatgggca ggctcagccc gccccgggca gccagcagca
     1141 ccgtcagcgg gatggcgacc aggctggcga tctgatccag ccgggcggca gacaggtaag
     1201 tgacggcggc gcgctcctcg ccaatctcgt cgctgatcca ggcctcggct gcgccgctgg
     1261 tgaaggtgta gcccaggccc cacagcacct gcgccagcag aatggtggcg aaatagggcc
     1321 agccgccgta cacgatgaag cccacaccca ccagcgcaaa cccgatgatg accgacaggc
     1381 ggcggctgaa caggtcggcc accacgccgg tgggaatttc aaaaataaag gccgaaagct
     1441 cgagcacagt gcccaccagc accaactgca gcggctccaa ttttgccacg gtgatgacga
     1501 acaggttgct agcggtaaag accagggtgt aacacagcgc ggccgcggcg ttcataatca
     1561 aatagagccg ccgggcttct attttcaggg cggggattcc gggacggtgc atggctgcga
     1621 ttgtaacatg aagccgcggc gcccggaacc tgcccccttt gttctggatg gattgaagtc
     1681 catgttgttc ggatgtacaa tgaaatcggc ggagcgtctg gtgaaaaata aattctggct
     1741 ggctgcgggc acactgattt taatcatggg catggggctg ccgatgggga ttcaatttgc
     1801 agccgggcag ccctccgaac caccgcaagc ctccgggccg ccgccggtct gtctgcccgg
     1861 cgagccctgc cttgatgaga gcgggcgctg ggtcttggcg gaagatgaca gcccagacgg
     1921 cgctgtgctg tccgggttcc aatctacggg cgggccggat gattatggct acacctggga
     1981 tgacagcgtg acctacgcct gggtctatgc caccaacggc accgatgccg gattttcggg
     2041 cagcagcccg cagttgaccg ggccgattgc gctgccgttc aactttacgt tttacggcca
     2101 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccgtccac ttatgtgggt
     2221 cagaaggcat tgaagacgcc gacgggctgg atgggctggc ctattatccc ttctgttacc
     2281 cggtttcgga cctcaaggcg gtgcgctttt acaaaccggc cgcactggtg cgcaccaggg
     2341 tgtacccgct ttatgacggt cagttgacgc agcccggtca aaccaacacc tttacgttca
     2401 cggtgggcaa caccggcgat tttggtcccg atacctatga tctgaccgtc gcttctgcct
     2461 gggcagtgag cctgtatggg ccggacggca tcacgcccct ggacgatacc gatggcgata
     2521 cccgcctgga tacgggcgcc attccgccgg gggaagtcat caacatcatc gcccgggtga
     2581 ctgcgcccga cgggctgagc ccgggcgccg aaaacacagc cagcatcact gtcaggtcgt
     2641 ctttgaatac gaccatcgct aagactgtgc agctcgaaac caccatcccg gcgcagtttg
     2701 cccaggcata ccgcgatcaa gaccccggca gtgtaaatct ggacctgaac tgggccgctc
     2761 cgcagctcac gctggcggtc acgccgggcg aagatggtca ccggtacgag cctgccgtgg
     2821 tgcaaaccac cgaccagcat tttgttacgg tgtgggccga atggcaggaa accggcgggc
     2881 agcagggtta ccggctgctg tctgccaggt tgaatcagta cggtcaggtc atccgcccgc
     2941 cggctgcttt gaccgcgctg gagccggggg tagatttttt cagcgatggc tcgattgccg
     3001 cggcggctgc gccagatggc cggattggga ttgcctggta ccggctgcgg cgcatcgatg
     3061 ccggtgggct gtctttctat aactacaata tctggtttgc cgtcctcaac cccgatggca
     3121 gtctggctgt cggcccggtc aatttgaccc agcagaccac ctggcaggtg gacgaggacg
     3181 atgacctgca caaagtttat gcgcccaccc tggcagtcag cagcgacaac cgtttttggg
     3241 tgagctggga gcagtattac cgccccaccg gcgcaccgtt gtacgagatc tattccacca
     3301 ttttggcggc tgatggcagc gtggtgatgc cgatcacccg gatggcgagc gggattccgg
     3361 gcagcctgcg ctaccgtcac cccagcgtgc tggcactgag cggcagccgc tttttgctgg
     3421 cgtatgatta cctcaactac gcacctttcg tcagcagcgt aaagtatcgg gtctatgaca
     3481 gcagcggcga aggactggta tctgagcggg ataccggctt ttatggcctg cagattgatg
     3541 ccgcgcagct ttctggcggg aacgtgctga ttgccgccga cgcccgcaac catatccatt
     3601 atgcggtcat caatggcagc agcctgattc cgccggtcat ttacggtacg ctgcagcatc
     3661 catctgattt agatgaaagt ttcgctgtgg cggttgccgc cgatgagact gatcacgcca
     3721 ttttgacctg gcaggatggc cgcggccaat atctgtatta cgccctggta gatgcatctg
     3781 gggcggcggt cagcgggcca gccatctacc gcacagcacc agacagccag gcctggcccc
     3841 gcctggaaac cagcgccaca ggctacagcc tgaccaccaa cgcgtggacg ccgccagccg
     3901 atgtggacgg actgaccggt ttctcagccg gctcctacgg cgcgcaggct ggcggggcgg
     3961 ctgtggtggg gctgcactat gccaaccagg ggcttacccc ggcagccagt cccgtgctga
     4021 cgctgacgct gggcgatggc ctgtcttacc tgagcgatac ctctggcctg tcggcggccg
     4081 tcaatggatc agagatcacc tggaacctgc ccgacctgga tttcggtgcc cacgaccaat
     4141 ttacggttta tattggcctt ccagcaggcg cccccctggg gacaacctat cccctcagcc
     4201 tggcgctgga atccgaaggc cccgaggccg acgcgaccaa caatgccgac acagcagagg
     4261 ttgtggctgc gcggcaaatg tggctgccag cgatccagcg gtagcccgag ggcggcttca
     4321 ggcggcgggc ggctgggcgg gtttgcgcgg ggcgaagatg atgcggatca gatggatgat
     4381 gccgcgcacc agcgccgtga cgggcacgat cacccagtta atcagttcgg agaagatacc
     4441 aaagccctgc cccacccaga caatcgccgg gcgcagtttg tgcagcggct tgatgtgagc
     4501 ataggccagg cagagcgcca cggtcaggag cgagatggcg aagcgctgcc agtcagcaaa
     4561 ttcgaaaatg ccaaattctt ccagcagcag ttcaatgccg atgttgagga tcaggatgta
     4621 ggcggcggtc ttgaggatgg gttctttttg cacggcacgg ctgaacaacc cggcggcaaa
     4681 gcgcatcatc acaatcccga tggccacacc cagcatgacc acccacagct tatctgagag
     4741 cgaaactgcc gtgaccacgt tatccagact gaagaccagg tcggccagtt ccaccgagac
     4801 caccaccgac cagaagctgc cggtcttgag gcggcggatt tcgccttctt cggtctccac
     4861 atctactgtg cccaggtttt caaaggccag gtgcatcagg tagagggcgc caatgatttt
     4921 gagccacgga ttgttgacga tgaggctggc cagcagcagc atcacgccgc ggccgacata
     4981 ggcgcccagc aggcccacgc gcaatgcggc agtgcgctgg ttgcccagca ccttgtccag
     5041 catgtggccc accttgcgca gcgcaggggg ccaatcaatc gggcggtcat ccggcagggg
     5101 tgtgaccatg gcgccgagca ccgcggcatt atcaattgag agcagccctt ccagcacaat
     5161 aagctggagg ataatcaatg ggatagtcca gtccatagat cctcttaagc ggtcaacaaa
     5221 ccaacatttg gcactttagt gaataccggg attatactct gataaatatg attaatatag
     5281 tgatttatt
//
LOCUS       DAOPVS010000132         5286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15608_length_5286_cov_55.787612, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000132 DAOPVS010000000
VERSION     DAOPVS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15608_length_5286_cov_55.787612"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..2259
                     /locus_tag="PKL11_09125"
     CDS             19..2259
                     /locus_tag="PKL11_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13803.1"
                     /translation="MLKLFKYLKPYLAMILVTIVLLFIQANAELALPDYLSRIVNVGI
                     QQGGVENAAPVAVRQSQMTHMLAFLDAADQTRLLADYTLVDQSSPDYVAQLAVYPALE
                     NEPVYILKDVDETELEWLNSKAGQTLLVVSGLEQMLADPAQAAAMSQKLGFDLTMLPP
                     GTDIYTVLGQLPAEQRAQIRAAIEQQFAALGESMVNQAAIRATRAEYTALGMDPGQFQ
                     NTYIFKTGGWMLLISLVTVICAVGVSYFASRSAAGFARDLRKSLFQRVETFSSAEFNK
                     FSTASLITRTTNDITQVQMFVLMMMRMIFFAPIMGVGGIIRAMSKGSSMWWIIAVAVA
                     VLLSLIFVVFSLSLPKFKIMQSLIDRLNLVMRENLSGMMVIRAFNTQQIELNRFDKAN
                     VNLTDVNLFVSRVMATMFPAMMLIMNGLSILIIWVGAHQVAQATMQVGDMMAFLQYAM
                     QIVFSFLMMSFMFIILPRASVSANRIADVLDTIPTINDPETPQTFAQPFRGVVEFQNV
                     SFRYPGADEDVLHDISFTAYPCQTTAFIGSTGSGKSTIINLIPRFYDVTEGTILLDGM
                     DIRTVTQHDLRSNIGYIPQQSTLFSGTIESNLRYADENASQEILDQAVEIAQAAEIIA
                     AKPEGWQTEIAQGGVNVSGGQKQRLSIARALVKNAPIYIFDDSFSALDFKTDAALRRA
                     LNEKSGCSTLLIVTQRVSTIIHADQIIVLDEGKIVGKGTHSELMTSCETYREIALSQL
                     SMEELS"
     gene            2256..4136
                     /locus_tag="PKL11_09130"
     CDS             2256..4136
                     /locus_tag="PKL11_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13804.1"
                     /translation="MTTPNRPQQPPRGGPGGHGGPMGGGPRMMMPGEKARDFKGTMRK
                     LLQFLKPYRVSILIVMVFAAFSTIFSIIGPKILGKATTRLFEGVLAQISGTGEGIDFV
                     YIGNILLIMVGLYLLSSLFAYIQGWIMSGIAARITYRFRKDLSEKINRMPLKYFDGTN
                     HGEVLSRVTNDVDTVSQTLNQSLSQILSNIVTVIGVLIMMISISWQMTLIALLTIPIS
                     LVITGLVIKNSQKFFKQQQEYLGHVNGHVEEMYGGHIVMKAFNGEAQSVVKFDNFNST
                     LYSSAWKSQFLSGLMMPVMGFVGNLSYVAVAILGGTLAIRNLITVGDIQAFIQYVRSF
                     TQPISQLANISNILQQTAAAAERVFAFLEEPEEVAETADSVKLTAVSGRVEFEHVCFG
                     YDPEKIIIKDFSASIAPGQKIAIVGPTGAGKTTLVKLLMRFYDVNCGAIRVDGHNVKD
                     FTRADLRDMFGMVLQDTWLYNGTIMDNIRYGRTDATDEEIYAAARAAHVDHFVHTLPE
                     GYQMVLNEETTNISQGQKQLLTIARAVLSDPKILILDEATSSVDTRTEILIQKAMDNL
                     MKNRTSFIIAHRLSTIRNADLILVLNEGDVVEQGTHAELLARNGFYAELYNSQFEHLS
                     AA"
     gene            4158..5258
                     /locus_tag="PKL11_09135"
     CDS             4158..5258
                     /locus_tag="PKL11_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13805.1"
                     /translation="MTSSPTHTLGQVALIGSGETAANGGRAFEVLASALPVPLEIAVL
                     ETPAGFELNSDRVAGRVVDFMSVRLQNYRPQIALIPARRRQPPFSTDDTAILAPLSHS
                     HLIYMGAGSPSYTVRQLSGSLAWHMILGRHLLGASLALASAATIALGAWALPVYEIYK
                     VGEDPHWKPGLNFFGRFGLSLAVIPHWNNTDGGAELDTSCCFMGRQRFDLLAAMLPAE
                     TTILGIDEHTTLLFDLSAGQGRVLGTSAVHIRRAGQERFFESGETFPLTELGPFKLPV
                     NPWADLPAEIRPIFESARQERQQQQCLNETPPPEALALLEARQQARSRREWSLADSYR
                     AQLAALGWQVQDTPTGPRLEPLRSSSDSCRSS"
BASE COUNT         1121 a         1656 c         1271 g         1238 t
ORIGIN      
        1 acaaggagcc ccacattcat gcttaaactg ttcaaatatc tgaagcctta cctcgccatg
       61 attctggtca ccattgttct cctgtttatt caggccaatg cagaacttgc cctgccagat
      121 tacctttccc ggatcgtcaa tgtcggcatc cagcaggggg gcgttgaaaa cgccgctcct
      181 gtggctgttc gccagagcca gatgacacat atgctggcct tcctcgacgc cgctgaccaa
      241 acccgcctgc tggctgatta caccctggta gatcaatcct ctcccgatta tgtcgcccag
      301 ctcgcagttt atccagcact tgaaaacgag ccagtctaca tcttgaaaga tgttgatgaa
      361 actgagttgg aatggttgaa ctcaaaagcc gggcagacct tgttggttgt ctctggcctt
      421 gagcaaatgc ttgccgatcc agcccaggcc gctgccatga gccagaaact tggctttgac
      481 ctcaccatgc ttccccccgg aaccgatatc tacacggtgc tgggccaact ccccgctgaa
      541 cagcgcgccc agatccgcgc cgccatcgaa cagcagtttg ctgccctcgg cgaaagcatg
      601 gtcaatcagg ctgccattcg cgcaacccgg gcggaatata ccgccctggg catggaccct
      661 ggccagttcc aaaataccta tattttcaaa accggtggct ggatgctgtt gatttccctg
      721 gttaccgtga tttgtgcagt gggcgtcagc tactttgcct cccgctctgc cgccggcttt
      781 gcccgcgacc tgcgcaagtc gcttttccag cgggtcgaaa ccttctccag cgccgagttc
      841 aacaaattct ccaccgcttc gttgatcacc cgcaccacca acgacatcac ccaggtgcaa
      901 atgttcgtcc tgatgatgat gcgcatgatt ttctttgccc ccattatggg cgttggcggc
      961 atcatccgcg ccatgagcaa gggttcttcc atgtggtgga tcattgctgt ggctgtggcg
     1021 gttctcctca gcctgatttt tgtagtcttt tcgctgtcgc ttccaaaatt caaaatcatg
     1081 cagagcctca ttgaccgcct caatctggtc atgcgtgaaa acctctccgg catgatggtc
     1141 attcgtgcct tcaacaccca gcaaattgaa ctgaaccgct ttgacaaagc caatgtgaat
     1201 ttgaccgatg tcaatctctt tgtcagtcga gtgatggcaa ccatgttccc tgccatgatg
     1261 ctcatcatga acggtctgtc catcttaatt atctgggtgg gcgcccatca ggtagcccag
     1321 gccaccatgc aggtcggcga tatgatggcc ttcctgcagt atgccatgca aattgtcttc
     1381 tcattcttga tgatgtcctt catgttcatc atcctgccgc gggcatctgt ctctgccaac
     1441 cgcatcgccg atgtcctgga taccatcccc acgattaatg atcctgaaac tcctcagacc
     1501 tttgcccaac ccttccgcgg tgtggtcgaa ttccaaaacg tctccttccg ctaccccggc
     1561 gccgatgaag atgtgctgca cgatatctct ttcactgcat acccttgcca gaccacagcc
     1621 ttcatcggtt caaccggctc aggcaagtcc accatcatca acctcatccc ccgcttctat
     1681 gatgtgacgg aggggaccat tttgttggat ggcatggata ttcgcaccgt cacccagcac
     1741 gatctgcgca gcaatattgg ctacatcccc cagcaaagca ccctcttttc tggcactatt
     1801 gaaagcaacc tgcgctatgc agatgaaaac gcctcgcagg aaatccttga ccaggctgtt
     1861 gaaattgcgc aggcggctga aatcattgcc gccaaacccg aaggctggca gaccgagatt
     1921 gcccagggcg gtgtcaacgt ctctggcggt cagaaacagc gcctgtccat tgcccgggct
     1981 ttggtcaaaa acgctcccat ctacatcttt gatgacagct tctcagccct ggacttcaag
     2041 acggacgctg ctctccggcg cgcgctgaat gaaaaatcgg gctgcagcac gctgctcatc
     2101 gtcacccagc gcgtctccac catcattcat gccgaccaga tcatcgtcct ggatgaaggc
     2161 aaaattgttg gcaaaggcac ccactcggaa ttaatgacat cctgtgagac ctaccgcgaa
     2221 attgcgctct cacagttaag catggaggaa ctctcatgac aaccccaaat cgtcctcagc
     2281 agcctccccg cggtggaccc ggcggccatg gcggccccat gggtggcggc ccgcgcatga
     2341 tgatgcccgg tgaaaaagcc cgcgacttca agggcaccat gcgcaagctg ctccaattcc
     2401 tgaagcccta tcgggtttct atcctcattg tgatggtttt tgccgctttc tccaccatat
     2461 tcagcatcat tggccccaaa atcctcggca aagccaccac ccgcctcttt gaaggcgtcc
     2521 ttgcccaaat ctccggtacc ggcgagggca tagactttgt ctatatcggt aacatcctcc
     2581 tgatcatggt cggcctgtac ctcctgtcat ccctgttcgc ctatattcag ggttggatta
     2641 tgtccggcat cgccgcccgc atcacctacc gcttccgtaa agacctctcc gagaaaatca
     2701 accgcatgcc gctcaaatac tttgacggca ccaaccatgg tgaggtgctt tcacgcgtga
     2761 ccaacgatgt ggataccgtc agccagacac tcaatcagag cctgtctcaa atcctctcta
     2821 acatcgtcac cgtcatcggc gttttgatca tgatgatctc catcagttgg cagatgaccc
     2881 tgatcgcctt gctcaccatc cccatctcac tggtcatcac cgggcttgtg attaagaact
     2941 cacaaaagtt cttcaaacag cagcaggaat acctgggtca cgtcaacggt catgtggaag
     3001 aaatgtacgg cggccacatc gtcatgaagg ccttcaacgg cgaagcccag agcgtggtta
     3061 aattcgacaa cttcaatagc acactctaca gttcagcctg gaagtcccag ttcctgtccg
     3121 gcctgatgat gccggtcatg gggtttgtgg gtaacctcag ttacgttgcc gtggcaattc
     3181 ttggcggcac cctggccatt cgcaacctca tcaccgtggg tgatatccag gccttcatcc
     3241 agtatgtccg ctcatttacc cagccgatct cgcagcttgc caatatctcc aatatcctgc
     3301 agcaaaccgc tgccgcggcc gaacgtgtct ttgccttcct cgaagagccc gaagaagttg
     3361 ccgaaaccgc tgattctgtc aaactgacgg ccgtctctgg ccgggtggag tttgagcatg
     3421 tctgctttgg ctacgacccc gaaaaaatca tcatcaaaga cttctcggca tccattgccc
     3481 ctggtcagaa aattgccatt gtcggcccta ccggcgccgg caaaaccact ctggtcaaac
     3541 tgctgatgcg cttctatgat gtcaattgtg gcgccatccg tgtggatggg cacaacgtaa
     3601 aagatttcac ccgcgccgat ttgcgcgata tgtttggcat ggtgttgcaa gacacctggc
     3661 tctataatgg aactatcatg gacaacatcc gctatggccg caccgacgcc accgatgagg
     3721 agatctatgc cgcggctcgc gccgcccacg tggaccattt cgtccacacc ctgccagagg
     3781 gttatcagat ggtactcaat gaggagacca ccaacatttc tcaaggccag aagcagttgc
     3841 tcaccattgc ccgcgcggtt ttgtccgatc ccaaaatcct catcctggat gaggccacca
     3901 gttcggtcga tacccgcact gaaattctga ttcaaaaggc catggataac ctgatgaaga
     3961 accgcaccag ctttatcatc gcccatcgcc tttccaccat ccgcaatgcc gacctgattc
     4021 tggtcctcaa cgagggtgac gtggttgagc agggcaccca tgctgaactg ctggcgcgca
     4081 acggtttcta cgctgagctg tacaacagcc agtttgaaca cctttcggca gcataatcaa
     4141 caggcagggt tgacgcagtg acatcctcac ctactcacac actgggccag gttgccctca
     4201 ttggctccgg cgaaaccgcc gccaatggcg gccgagcctt tgaggtgctt gcatcagccc
     4261 tgccggttcc gcttgaaatt gccgtccttg aaacccccgc cggctttgaa ctcaattctg
     4321 accgggttgc cggccgcgtg gttgatttta tgtccgtccg gctgcagaac taccgtccgc
     4381 agatcgccct catcccggcc cggcgccgtc agcccccctt cagtaccgat gacaccgcca
     4441 ttctggctcc gctctctcac agtcatctaa tctatatggg cgctggcagc ccttcctata
     4501 cagtgcggca gttatccggc agcctggcct ggcatatgat ccttgggcgc catctactgg
     4561 gggcttcgct ggctctggct tctgccgcca ccattgccct tggcgcctgg gcgttgcctg
     4621 tctatgagat ttacaaagtc ggcgaagacc cgcactggaa gcccggcctc aacttctttg
     4681 gtcgttttgg cctctcactg gcagtcatcc cgcactggaa caacaccgat ggcggcgccg
     4741 aactggatac aagctgctgt ttcatgggcc gccagcggtt tgatctgctt gcagccatgc
     4801 tgcctgccga aaccacaatc ctgggcatag acgaacatac caccctgctg tttgatctgt
     4861 ctgccgggca ggggcgtgta ctgggcactt ctgctgtaca catccggcgt gccggtcagg
     4921 aacgtttctt tgaaagcggc gaaactttcc cgctgacaga actggggcct ttcaaattgc
     4981 ctgtcaatcc ctgggccgat cttcccgctg aaatccgccc catatttgaa tccgcccgcc
     5041 aggaacgcca gcagcagcaa tgcttaaacg aaacaccccc gcccgaagcg ctggccttgc
     5101 tcgaagcacg gcaacaagcg cgctcccggc gggagtggtc gttagcagat agctatcgcg
     5161 ctcaacttgc ggcgttaggc tggcaggtgc aggatacacc caccggcccc cgccttgagc
     5221 cgcttagatc cagttctgat tcttgcagaa gttcgtgata acggggatat tgacgtactc
     5281 accctt
//
LOCUS       DAOPVS010000133         5202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_15972_length_5202_cov_39.178551, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000133 DAOPVS010000000
VERSION     DAOPVS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15972_length_5202_cov_39.178551"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1591
                     /locus_tag="PKL11_09140"
     CDS             122..1591
                     /locus_tag="PKL11_09140"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ13806.1"
                     /translation="MKSLRSILGGLASALITGLIALGALTLSITEGNMWLPAPTEAGP
                     AAATATPGETLAIETLSANPTQAGETQIQPTPTLPLTAPTAMPGCLPPQGWVQHYIQP
                     GETLAGLASQYQITQDTLRSANCLFTDTLLAGTFIYLPPGQIILPTVTATTIPCGPPR
                     GWIQITLSAGNTLYSLSRDYNVSVYELQLANCMGYSTQLYAGAKFWVPNVPTRTPTAT
                     EVLPPTHTPPPPTVTLTPTVPSVYGVSLTPAADAQSGDPGTNVNYVLVLTNTGNVSDT
                     FTVSTSGASWTSVAAPSAVGPLAAGATQNITVTVSIPAGAAGGSTDTVTVTAVSQASG
                     TVSDSAALTTTANTLASYGVSLSPAADSASGSPGADVLYTLTVTNTGNTSDYFDLSVS
                     GAVWTTTIAPMTVGPLAAGTSQEITVSVSIPDDASNGTTDTATVTAVSQGDTTATATA
                     SLTTTASVKAGLGLPIYWAETGTFPATTASHNTDSRQAL"
     gene            1635..2645
                     /locus_tag="PKL11_09145"
     CDS             1635..2645
                     /locus_tag="PKL11_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ13807.1"
                     /translation="MTKIPKPIFWGMLVIVLTMVACNFPVQPGGQAGNPVWINDPNAT
                     VTPTPFQPMPPTPESPNEMVIPTADAYTPGQTPTAPSADNLIKILVLGSDMRQSLDYR
                     TDVFMLVLINPQNGTISVVSFPRDLYVEMPNFGTDRINTAMEFGGFELTAATLEYNFG
                     IRPDYYMLTNFRGFTAIVDSLGGIDVNAAVNLTDQCKLPQAYDGYCSVGPGWVHMDGQ
                     TALWYVRSRYSTSDFDRGRRSQEVMEALFTRLMSLDAVSRIPELYQIYQQNVETNVPL
                     DVVVTLGGIAPGLIADSTRIHRYAVGPGETYPYMTPGGASVLLPNYEAIQLILNRAIL
                     GE"
     gene            2817..3725
                     /locus_tag="PKL11_09150"
     CDS             2817..3725
                     /locus_tag="PKL11_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNZ13808.1"
                     /translation="MTLQSSPEVHISPQTLLPPALRAWEIYLRDQGRSSNTIKAFMGD
                     LQLLADFLPPDQSIGSVTTRDLNHFLQWLQNGRGVPCSPKSLARRITSVKAFFRWLQK
                     HGRITTDPAEKVLQQSVISPLPEVLTAEEQQKLIQAALGMRQARKPDARPYALLTLLL
                     STGIKKGECLNLSINHIDLEAPNGPVVFIRYAGSQNRYKERRIDLTADWIESYHEYMA
                     QYTPRDQVFPWSPRRLEYLLEDLGTEAGLVKHVSFDMCRWTCALNDYQSGMQPDAIRQ
                     KLGISKIQWREIGLKLRKLAGDITES"
     gene            complement(3722..>5202)
                     /gene="tilS"
                     /locus_tag="PKL11_09155"
     CDS             complement(3722..>5202)
                     /gene="tilS"
                     /locus_tag="PKL11_09155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNZ13809.1"
                     /translation="QAHYPLNLDARGYNAFMHLARYEKICRENCRLQPDQVILVGLSG
                     GADSLCLADLLDRLGYPLAVAYFNHGLRPEAAHEAETIAQLATSRGWQYLSTSAPIAE
                     MARQQRLSIEAAARQARYTFLFDAARRVGAQAVAVGHQADDQVETVLLHLLRGSGLNG
                     LRAMRWRAIFPDWDPQIPLVRPLLGTWRTEIAVCCADRGLVPLEDATNAEQHFTRNRI
                     RAQLLPMLETYNPQIKSALWRLAFTLQGDFDILTSVVDTTFQSLLRQSDLGRVAMDHH
                     TLAELEDGLLAAVLRRAAQSACPSLDDLDFDAVQRSIVFVRQPIPQGRVELTSGLVIF
                     REGDQIILAQWDQLPPEPAWPQISEGSAIPIPVPGELSLPGGWFLTAQYLESARLSDL
                     RPNFGDPFACWLNAAALPGPLLLRTWQPGDRFNPLGMANKTVKLADYWTNVGLPVRAR
                     SNWPLVVSGSQIIWVPGYRPAHSSRLSEAPQAVIHLLLTKTG"
BASE COUNT         1200 a         1407 c         1571 g         1024 t
ORIGIN      
        1 aagcgctggt tgaggcgcac ggcgggcgca tctgggttga gagcgaaatt ggacagagtt
       61 caaccttcag cgtgctgctg ccgattgaaa aaccccggca gattggacga tttctaggcg
      121 aatgaagtcg ctgaggtcca tcctgggtgg tttggcatcg gcgctgatca cagggttgat
      181 tgccctggga gcactgacgc tttcaatcac cgaggggaat atgtggctgc cagcgccaac
      241 cgaagctgga ccggccgctg caacagccac gccgggtgag acgctggcaa ttgaaacgct
      301 gtccgccaac ccaacccagg caggggagac gcagattcag ccaaccccca cgctgccttt
      361 gacagcgcca acagccatgc ccggctgcct gcccccgcaa ggctgggtgc agcattatat
      421 ccaacccggc gagaccctgg cagggctggc aagccaatat cagattacgc aggatacgct
      481 gcgaagcgcc aactgtttgt ttactgatac attgttggcg ggtacattta tttacctgcc
      541 gcccgggcag attattttgc ccacggtaac agccacaacc attccctgcg gcccgccgcg
      601 tgggtggatt caaattaccc tgagcgcggg gaatacgctc tacagcctga gccgtgatta
      661 caatgtttca gtttatgaac tgcaactggc gaactgcatg ggatacagca cacagcttta
      721 tgccggggcc aaattctggg tgccgaatgt accaacccgc acccccactg caacggaagt
      781 gctaccgccc acccataccc ccccgccccc tacagtcact ttgacgccga cggttccatc
      841 ggtttacggg gtttctttga ccccggcggc cgatgcacaa agtggcgatc cgggcacaaa
      901 tgttaactat gtgctggttt tgaccaatac ggggaacgtc agcgacactt tcaccgtctc
      961 aaccagcggg gcaagctgga cgagcgtggc ggcgccatct gccgtggggc cgctggcggc
     1021 aggggcaacg cagaatatca ccgtaacagt cagcatcccg gcgggtgcgg cgggtgggag
     1081 caccgacacc gtgaccgtga cagcggtatc ccaggccagt ggaacggttt cagattcagc
     1141 agccttgacc accacagcca ataccctggc aagttacggc gtgtcgctga gtccggcggc
     1201 agactcggca tctggaagcc cgggcgcgga tgtgctctac accctgactg tgacaaatac
     1261 tggcaacacc agcgattatt ttgatctgag cgtcagcggg gcggtgtgga ccacaacgat
     1321 tgcacccatg acggttgggc cactggctgc cggtacgagc caggaaatta cggtcagtgt
     1381 gagcatccca gatgatgcat caaatggaac cacagacact gctacagtga ccgccgtatc
     1441 ccagggggat actacagcca cggcaacagc aagcctgacg accaccgcca gcgtcaaagc
     1501 aggattaggt ttgcccatct attgggctga aactggaaca tttcctgcaa cgacagcgtc
     1561 tcataataca gactcgcgac aggcgctgta accgcgtccg gcgataggat ttatagatag
     1621 gatttgcgcg cgctatgact aaaataccca agcctatttt ctgggggatg ctggtcattg
     1681 ttttgacaat ggtggcctgt aattttccag ttcagccggg aggacaggca ggaaacccgg
     1741 tgtggatcaa tgatccgaat gcaacggtga caccaacgcc atttcaaccc atgccgccaa
     1801 cgccggaaag cccaaatgag atggttatcc caacggcgga tgcatacacc ccggggcaga
     1861 ctcccacagc accaagtgca gataacctga tcaaaatcct cgtgctgggg tcagacatgc
     1921 ggcagtcgct ggattaccgc acggatgtct ttatgctggt attgattaac ccgcagaacg
     1981 ggacaatcag cgtggtgtct tttccgcggg atttgtatgt ggagatgccc aactttggca
     2041 cagaccggat caataccgcc atggagtttg gcggctttga attgactgcg gccacgctgg
     2101 aatataattt tggcattcgg ccagattatt atatgctgac caatttccgg ggatttacgg
     2161 caattgtgga cagcctgggc gggattgatg tgaatgcggc ggtcaacttg actgaccaat
     2221 gcaagctgcc acaggcgtat gatggctact gctcggtggg gccgggctgg gtgcacatgg
     2281 acgggcaaac ggcgctgtgg tatgtgcgtt cgcgatacag tacctcggac tttgaccgcg
     2341 ggcggcgctc tcaagaggtg atggaagcac tattcacccg cctgatgagc ctggacgcag
     2401 tcagccggat accggagctt tatcagattt accaacagaa cgtagagacc aatgtgccgc
     2461 tggatgtggt ggtgacgctg ggcgggattg cccctggttt gattgcagac tcgacgcgga
     2521 ttcaccgata tgcggttggc ccgggtgaaa cctaccctta tatgacgccg ggcggggcct
     2581 cggtgctgct gcccaattac gaagccattc agttgatatt gaatcgggcg attctgggcg
     2641 agtagagagc atcacggctg caccctgtgg gggcgatggg tgggttgaat ttcatattcc
     2701 aggggaaaat gtccaaaatt gtgctgcaaa atcatttgac agggtttttg atttattgta
     2761 aactggattt gttaggctat gcgaaaatcc tatacgaaag tcaggagact tgaattatga
     2821 ctctccagtc cagccccgaa gtgcatattt ccccgcagac gctgctgccc cccgccctgc
     2881 gcgcctggga gatttattta cgcgatcagg ggcgctcgtc gaacaccatc aaggctttta
     2941 tgggtgattt acaactgctg gcagattttc tgccgccaga tcaatcgatt ggcagtgtga
     3001 caacccgcga tttgaatcat tttctgcaat ggctgcagaa cggacgcggg gtaccttgca
     3061 gccccaagag cctggcgcgc cgcatcacct cggtcaaggc attttttaga tggctgcaaa
     3121 agcacggacg gatcaccacc gacccggcgg agaaagtgct gcagcaatct gtcatcagcc
     3181 cactgcccga agtgctgacg gctgaagaac agcaaaaact cattcaggcg gcgctgggaa
     3241 tgcggcaggc gcgcaaacct gatgcgcgcc cctacgccct gctgaccttg ctgctgagta
     3301 cggggatcaa gaagggtgaa tgcctcaacc tgagcattaa ccatattgat ctggaagccc
     3361 caaatggccc ggtagttttt attcgctatg cggggagcca gaatcggtac aaggaacgcc
     3421 ggattgattt gacggcggac tggattgaaa gctaccacga atatatggcg cagtacacgc
     3481 cgcgcgatca ggtttttccg tggtcacccc gccggttgga atacctgctg gaagacctgg
     3541 gtaccgaagc cgggctggtg aagcacgttt cgtttgatat gtgccggtgg acctgtgcgc
     3601 tgaatgacta tcaaagcggg atgcagccag atgccatccg ccagaaactg gggatttcga
     3661 agattcaatg gcgtgagatt ggcctgaaat tgcgcaaact cgctggagac atcaccgaaa
     3721 gttaaccggt tttggtcaat agtaaatgaa taaccgcctg gggcgcttca gacaaacggc
     3781 tggagtgcgc cgggcggtaa ccgggcaccc agataatctg tgaaccgctg acaaccagcg
     3841 gccaattgga gcgagcccgc accggcaggc cgacattggt ccaatagtcg gccagtttga
     3901 cggtcttatt ggccatgcca agcgggttaa aacggtcgcc gggctgccag gtgcgcagca
     3961 gcaatggtcc gggaagagcg gcggcattca gccagcaggc aaaggggtcg ccaaaattgg
     4021 ggcgcagatc ggagagccgg gccgattcca ggtattgagc cgtcagaaac caaccgccgg
     4081 gcagggagag ttctccaggt acaggaatgg ggatggcgga accttcagaa atttgcggcc
     4141 aggctggttc gggaggcaac tgatcccatt gcgccagaat gatttgatcg ccttcacgaa
     4201 aaatgaccag gccggaggtc agttccacgc gtccctgcgg gatgggctgg cgcacaaaaa
     4261 cgatggagcg ctggacggca tcaaagtcaa gatcgtcaag cgatgggcag gccgattgag
     4321 ccgcccggcg tagcacagcg gctaacaggc catcttccaa ttcggccagg gtatggtgat
     4381 ccatggcaac ccggccgagg tcagactgcc gcagcagcga ctggaaggtg gtatccacga
     4441 ccgaggtcag gatgtcaaaa tcgccctgaa gagtgaaggc cagacgccag agggcagatt
     4501 taatttgcgg attataggtt tccagcatgg ggagcagttg ggcacggata cggttacgag
     4561 tgaagtgctg ctcggcattg gtggcatctt ccagcgggac caggccgcgg tctgcacagc
     4621 agacagcaat ttcggtccgc caggtgccca gaagcggacg aaccagcgga atctgggggt
     4681 cccagtctgg gaaaatagcc cgccagcgca tggcccgcag accattgaga ccgctgccgc
     4741 gcagtaaatg cagcaagacg gtttcgacct gatcgtctgc ctgatggccg actgctacgg
     4801 cctgggcacc tacccggcga gcggcatcaa acaagaaggt gtagcgagcc tgacgggcgg
     4861 cggcttcgat tgagaggcgc tgctggcgcg ccatctcggc aatgggagca gacgttgata
     4921 gatactgcca gccgcgtgaa gtggccagtt gcgcaatggt ttcagcctcg tgggcagcct
     4981 cggggcgcag gccgtggttg aaataggcga cagccagcgg gtagcccagc cgatccaaca
     5041 aatctgccag gcagagggag tcggcgccgc cggaaagacc cacaagaatc acctgatcag
     5101 gctgcaagcg gcagttttcg cggcagattt tctcatagcg ggcaaggtgc ataaacgcat
     5161 tatacccgcg agcatcaaga tttaacgggt aatgggcttg cc
//
LOCUS       DAOPVS010000134         5189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16042_length_5189_cov_54.913518, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000134 DAOPVS010000000
VERSION     DAOPVS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16042_length_5189_cov_54.913518"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKL11_09160"
     CDS             <1..474
                     /locus_tag="PKL11_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ13810.1"
                     /translation="MNLVGPGSVIGHSLAMNNQPRLLGAAAQLPTVLLSIPRENLLHI
                     LETSRQLTFNLAIEFAEMQRKLLLHYQSFIMTGMEGRTALLLLHIGEGEKDELDLPIS
                     QTELAGWLGISRGGLNRTLIRFQKLGLICLEGSKIYILDRERLAALAEGLSVTDL"
     gene            533..829
                     /locus_tag="PKL11_09165"
     CDS             533..829
                     /locus_tag="PKL11_09165"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HNZ13811.1"
                     /translation="MKSSSEGLRLVPWFFLIFGFMTIESVQLVIGKAVLDADFRQLLL
                     KDPERALADFRLTNAEKQSLMKVDGETLEIMANAFEISQSVYLRYQLTHRNTTP"
     gene            892..>5189
                     /locus_tag="PKL11_09170"
     CDS             892..>5189
                     /locus_tag="PKL11_09170"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNZ13812.1"
                     /translation="MKVKPFGTSFFGVALVLALIASLMITPSAAAYQVPADPVITAGV
                     VIYVDRDATGANNGSSWTDAYTSLQAALTAASSGTEIWVAEGLYKPTTSTDRSASFNL
                     KNGVEVYGGFAGTETLRTQRDITAHQTILSGDLNGDDTTDASGVMLDWADTPRTESDD
                     NSYRVVMAWSVNSTAVLDGFTITSGFGDETISSFPYDVGAGLTGYQASPTLRNLVFRG
                     NEEDNGPGGAWYFWEGAPVMSDITVIGNAGCQGGAGYFREAPGSITNALFEANDAREL
                     SVSCGTGNVGGGGLYTIGNVPLTNVTFVNNTAVNSGGGLGTYGGTTLNNVSFTGNSAE
                     TGGGMEDGIGGSTLTDVQFIDNNASRFGGGMYTQSALATFTRVLFSGNHAGYGGGGLS
                     FNTTTGPVITDSKFIGNSAESVGGAMFLWNATAELTNVLISGNTAPYGSGAIYGQGGS
                     FTLTNVTIAGNSGTAVTAPGGVTARNTIIYGNGGALSGSPLTLSYSIIQGGCPGGATC
                     DHVTSSDPLFVAPITEPLPTTTGDMHLLSNSPAIDAGDNAVVTVTTDLDGSARFIDVA
                     ATPDTGSGTPPIVDLGPYEAFGFAPDLVASLENNVSGAAALNEPFNWTLTITNIGTGP
                     AEFSAAALLLHDDLPAAGATYGTPVVATSGVTGTISCAVSATPALICTASGAVSLDPG
                     SGSVTVTLPVTPTSFATLVNPAAAGICQADPDNSLTEIFETNNSCGDSVTVGVATLTG
                     VIHDAGHAAVTTAPIGAVIHGQATVAGPGSLVPTGTVTLSRYDSADCSSTAIASEDQP
                     LAGGVAEFSSMPASSFYYRYDYSGDSNHIPLESACTRFSAYQPGPIFTVNTTADDTLD
                     DICSDIHCTLREAINAANTTAGDNTILFEVGESQTLILTAALPAVSSINGALTIDGQT
                     HAITINGADSFRVFDASANAQLTLANLVVANGRHVGAECYGLSCGGGLKVSSGAMVTI
                     ENTILQNNTTQNLGGCIFNEGNLSIQDSTITGCLANEGGGIFNLGTLLIENSALISNT
                     AGYWGGSIYTEGSLTIVNSTLTGNSVSGTASNNGGGAIDQYGSGSVTIITNSTIADNS
                     AGSPEKSGFWMENGTMTIQNVLLAGNGTGNCLLAGGAFNAGTDSLSTDATCGSATQTS
                     DAGLGTLIGSPAYYPLDYDSPAVDLGDNTYCPATDQRGATRPVDGDRDGEARCDAGAY
                     EASYGPVDMDLSNTNLPENQPVGTVVGLLTTTDLYPGSIFTYTLVSGAGDTDNALFSI
                     TDNELLSAASFDFETQFTYSVRIRATDDGGLWYEEPFTITVTDGNDAPSIELTNTVTV
                     LPENADTSSAIQVAEIVVTDDSLGTNVLSLAGDDATLFEIVAGGLYLKAGIMLDFEIQ
                     PVLNVTVAVDDSTVGGEPDDTANLSINITDINEAPSVTLNNTITELPENANTSSA"
BASE COUNT         1195 a         1478 c         1426 g         1090 t
ORIGIN      
        1 atgaatttgg tagggccagg ttcggtgatt ggccattcat tagcaatgaa caaccagccc
       61 cgcctgctgg gagcggcagc gcaactgccc acagtgctat tatcaatccc gcgagagaac
      121 cttcttcata ttttggagac ttcgcgccag ctgaccttca atctggcaat tgagtttgcc
      181 gagatgcaga ggaagctatt actgcattat caatcattta ttatgaccgg gatggagggg
      241 cgtacagcat tactgctttt acatattggc gagggggaaa aagacgagct tgatctaccc
      301 atcagtcaga ccgaactggc aggctggctg gggatcagcc gcggggggtt gaatcgcaca
      361 ctcatcagat ttcaaaaact gggattgatc tgtctggaag gatccaaaat ttacatcctt
      421 gaccgggaga gattggcggc cctggctgaa ggactatcag ttactgattt gtaaaaaaga
      481 gaacaattta ttgtaatctg ggtgacatcc tgatcggggg aagaattgta caatgaaatc
      541 tagctcagaa ggtctcagat tagttccctg gtttttcctg atatttgggt ttatgacaat
      601 agaatccgtc cagttggtca ttggtaaagc tgttttggat gctgattttc ggcaattatt
      661 gcttaaagat ccagaacgag cattggcaga tttcaggctt acaaacgccg aaaagcaaag
      721 cttgatgaag gtggacgggg aaaccctgga aatcatggct aacgcctttg aaatatctca
      781 gtctgtttat ctgcgttatc agttgaccca tagaaacacg acaccataag cctgtgcggc
      841 ctcttttttt cttcaacgaa aaaggcaaat aaaaaatcag aaggagttcc tatgaaagtc
      901 aaaccatttg gcacctcatt ctttggcgtt gctttggtac tggctctgat tgccagcctg
      961 atgattacgc ccagtgctgc tgcttatcaa gtcccagccg accccgtcat cacagccggg
     1021 gtggttattt acgtagatcg ggatgcgacc ggagctaata acggctccag ttggacagat
     1081 gcctacacct ccctccaggc ggcacttacg gcagcttctt ctggtacgga gatctgggta
     1141 gctgaggggc tctacaaacc cactaccagc accgaccgca gcgccagctt caacctcaaa
     1201 aacggggtcg aggtttacgg cggctttgcc ggcaccgaaa cactgcgcac acagcgcgac
     1261 atcaccgccc accagactat cctgagtggg gatctgaacg gtgatgacac caccgacgcg
     1321 agtggcgtga tgctggactg ggccgacacg ccccgcacgg aatccgacga caactcctac
     1381 cgcgtcgtca tggcctggtc ggtgaactcg accgccgtgc tggatggatt caccatcacc
     1441 tccgggtttg gcgatgaaac catcagcagc ttcccctacg acgtcggtgc cggtctgacc
     1501 ggttaccagg cttcccccac cctgcgcaac ctggtgttcc ggggcaacga agaggacaac
     1561 ggcccgggcg gcgcctggta tttctgggag ggggctccgg tgatgagtga catcaccgtg
     1621 atcggcaacg ccggctgcca gggaggcgcc ggttatttcc gcgaggcccc cggatcgatc
     1681 accaacgccc tgtttgaggc caacgacgcc cgggagctgt cggtcagttg cggcaccggg
     1741 aatgtcggcg gcggtggtct gtacaccatc ggcaatgtgc cgctgaccaa tgtcaccttt
     1801 gttaataaca ccgctgttaa ttcaggcggc gggttgggga cctacggcgg gacgaccttg
     1861 aacaacgtct ccttcaccgg caacagcgcg gaaaccggcg gcggcatgga ggacggcatc
     1921 ggcggctcga ccctgaccga cgtccagttc atcgacaaca acgcctcccg tttcggcggt
     1981 ggcatgtaca cccagagcgc gctagccaca ttcacccggg tgctgttttc cggcaatcac
     2041 gccggctacg gcggcggcgg gctcagcttt aataccacca ccggcccggt gatcacggat
     2101 tcaaaattca tcggcaactc agccgaatca gttggtgggg ccatgttcct gtggaacgcc
     2161 accgccgagc tgaccaacgt cctgatcagc ggtaacacag ccccttacgg cagtggcgcc
     2221 atttacggtc aaggcggcag tttcacgctc acaaacgtca ctatcgccgg caacagcggg
     2281 accgcagtga ccgcccctgg cggggtgacc gcccgcaaca ccatcattta cggcaacggt
     2341 ggagccctga gcggctcccc gctgaccctc tcctacagca tcatccaggg cggctgcccg
     2401 ggtggggcca cctgcgacca tgtcaccagc agcgatccac tgtttgtggc ccccatcacc
     2461 gaaccgctgc cgaccaccac cggcgacatg cacctgctct ctaactctcc ggcgatcgat
     2521 gccggcgaca atgcggtggt gacggtcacc accgatctgg atggcagcgc ccgcttcatt
     2581 gatgtggccg cgacacccga caccggcagc ggcaccccgc ccattgtgga cctggggccc
     2641 tacgaagcct tcgggttcgc tcctgacctg gtcgccagcc tggagaataa tgtcagcggt
     2701 gcagcagctc tcaacgagcc gttcaactgg accctgacca tcaccaacat cggcaccggc
     2761 ccggcggagt tttccgccgc agcgctgctg ctgcatgatg acctgccggc ggccggggca
     2821 acctacggca cgcccgtggt ggccaccagc ggcgttaccg gcacaatcag ctgtgcggtc
     2881 agcgctacgc ccgccctgat ctgcacagcc agcggtgcgg tcagcctgga cccgggcagc
     2941 ggcagtgtga cagtcacgct gccggtcacc ccgaccagct ttgcaaccct ggtcaacccg
     3001 gctgcggccg gcatctgcca ggccgatccc gacaacagcc tgaccgaaat ttttgaaacc
     3061 aacaacagct gcggcgacag cgtgacggtg ggtgtggcta cgctgacggg tgtgatccat
     3121 gatgcgggac atgccgctgt taccactgcg ccaattgggg cagtcattca tgggcaggca
     3181 acggttgccg ggccaggaag ccttgttccg acggggacgg tgacattatc tcgctacgac
     3241 agcgcagatt gcagcagcac agccatagcc agcgaagatc agccactggc aggaggtgtt
     3301 gcagagttca gctcgatgcc tgcctccagc ttctattacc gctatgatta cagcggggac
     3361 agcaatcata tcccgctcga aagcgcctgc actcgcttct cggcttacca acctggccct
     3421 attttcaccg tcaataccac tgccgatgat acgcttgacg acatctgcag cgatattcat
     3481 tgcacactgc gcgaggcaat caatgcggca aataccacgg ctggtgacaa taccatcttg
     3541 tttgaagtgg gtgaatcgca gaccctgatt ttgaccgcgg cattgcctgc tgttagttca
     3601 atcaacggcg cgttgaccat cgatggtcaa actcacgcca tcacgatcaa cggcgcggac
     3661 agcttccggg tatttgatgc gtctgctaac gcccaactta cgctggcaaa cctggttgtg
     3721 gcaaatggac ggcacgttgg ggctgagtgt tatggtcttt catgcggcgg cgggcttaaa
     3781 gtctccagcg gggcaatggt gacgattgag aacaccatcc tgcaaaataa taccacccaa
     3841 aacctgggcg gctgcatatt taacgaaggc aacctgagca tacaagacag caccataacc
     3901 ggctgcttgg ccaatgaagg tggtggcatc tttaaccttg gaacgctgct gattgagaac
     3961 agtgcactca tcagcaatac ggcagggtac tggggcggca gcatttacac tgaggggagt
     4021 ctaacaattg tcaacagtac gctaactggc aacagtgtat cagggactgc atccaataac
     4081 ggcggcggtg cgattgacca atatggatct ggatcggtaa ctataattac caacagcacc
     4141 attgcagata acagcgctgg cagtcctgag aagagcggct tctggatgga aaacggcaca
     4201 atgaccattc aaaacgtgct tctggcaggt aatggcacag gaaattgtct gcttgccgga
     4261 ggcgctttca atgctggaac agactcctta tccacagatg ccacctgcgg cagcgccact
     4321 caaaccagcg acgctggatt gggaacgctc attggcagcc cggcttacta cccattggac
     4381 tatgactcgc cagcggtgga cctgggtgat aatacctact gcccggcaac cgatcaacgc
     4441 ggcgcaactc gcccagtgga tggtgaccgg gatggcgaag cacgctgtga tgcaggagcc
     4501 tacgaagcca gctatgggcc agtagatatg gacctctcca atactaacct ccccgaaaat
     4561 cagccagtcg gcactgtggt ggggttactc acgactactg atctatatcc gggcagtata
     4621 ttcacatata ctctggtttc tggcgctggt gatacggaca atgccctgtt cagtattaca
     4681 gataatgaac tgctcagtgc agccagcttt gattttgaaa cccagttcac ctattccgtg
     4741 cgtattcgcg ccacagatga tggcggattg tggtacgagg agccttttac cattacagtt
     4801 actgatggta atgatgctcc gagtatagaa cttactaata ctgtcaccgt cttgccagag
     4861 aatgcagata ccagtagtgc aattcaagtg gctgagattg tcgtaacaga tgattcgctt
     4921 ggcaccaatg tattgagcct tgcgggcgat gatgcaacct tgtttgagat cgtcgcgggt
     4981 ggtctgtacc tcaaagctgg tatcatgttg gactttgaaa tccaacccgt gttgaatgtg
     5041 accgtggctg tagatgacag cactgtcggc ggcgagccag atgatacggc caacctatcc
     5101 atcaatatca ccgatatcaa cgaagcaccg agcgtgacgc tgaataatac catcaccgaa
     5161 ctaccagaga acgccaacac cagtagcgc
//
LOCUS       DAOPVS010000135         5136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16261_length_5136_cov_52.035032, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000135 DAOPVS010000000
VERSION     DAOPVS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16261_length_5136_cov_52.035032"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1627)
                     /gene="guaB"
                     /locus_tag="PKL11_09175"
     CDS             complement(146..1627)
                     /gene="guaB"
                     /locus_tag="PKL11_09175"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ13813.1"
                     /translation="MNEDLFTAREALTFDDVLIVPGFSQVLPTDTDIRARLTDAIQLN
                     TPLLSAAMDTVTEARLAIALAREGGLGIIHRNLSPEAQAKEVETVKRSESGMIVDPIT
                     LPQTATLQEAEEIMSRFHISGVPIVDPQTQRLVGILTNRDIRFTGPEDYVRPVSDFMT
                     ATGLVTAPAHTTLEQAQAILQQHRIEKLPLVDPAGRLTGLITVKDILKKIEYPNASID
                     SRGRLRVGAAVGVGPDVEDRIERMTACGLDVVVVDTAHGHTAGVIRTIQRIKSVYPHL
                     PVIAGNVVTAEGTQALIDAGADVIKVGVGAGSICTTRIIAGAGMPQLTAIHACAQVAH
                     RQGVTVVADGGVKYSGDIVKAIVAGADVVMLGSLLAGLEEAPGEVIVYEGRQYKQYRG
                     MGSLGAMQGYGRDRYGTGQGKAGKLVPEGVEGMVPYKGTLADFVYQLVGGLRSGMGYA
                     GAASLTDLRTHTQLVRITNAGLIESHPHDVVITREAPNYRRSD"
     gene            complement(1705..3204)
                     /gene="purH"
                     /locus_tag="PKL11_09180"
     CDS             complement(1705..3204)
                     /gene="purH"
                     /locus_tag="PKL11_09180"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNZ13814.1"
                     /translation="MPKALLSVHDKTGLVEFASGLHALGWQLIASGGTARMLHDHNLP
                     VTEVADYTGSPEILGGRVKTLHPAIHGGLLARATEADHHELENLGWDYIDLVAVNLYP
                     FEQTIARPDVTLAEAIENIDIGGVTLIRAAAKNHQRVTLICDPADYPAALAALRAGGI
                     PADMRRNLAVKGFTQTAHYDSAITAYFSGSSALNLQLYPVQTLRYGENPHQQATLFSH
                     HPGEGPLGGQVLQGKELSYNNLLDLDASWRAVRAFESPSIVIVKHLSPCGMASADKLV
                     DAYRLALASDPISAFGGIVASNRPLDGETAEAIKDLFVECVVAPAFDPQALEIFSKKK
                     NLRLLVVPNTPVDPSVEYRSIHRGLLQQSIDLGDPFAAQWTVVSQRQPTEAEWRSLRF
                     AWKAAQHVKSNAIVFAQGEATVGIGGGQPNRVDCVRMAAQRSGERSRGAVMASDAFFP
                     FPDSVEVAIQAGITAIVHPGGSVRDAESIAAADAAGLAMVTTGVRHFRH"
     gene            complement(3223..>3632)
                     /locus_tag="PKL11_09185"
     CDS             complement(3223..>3632)
                     /locus_tag="PKL11_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNZ13815.1"
                     /translation="KLKDQDRNTYDARLADLVLSYQPDWVILAGWMRLLSMSFLSHFP
                     NRVVNLHPALPGAFPGTEAIARAFEAYQRGEIQSTGVMTHLVPDEGVDAGPVLAQIEL
                     PITPQDTLETLSERVHQAEHQLLVQTLQQLIQK"
     assembly_gap    3633..3732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3733..3968)
                     /locus_tag="PKL11_09190"
     CDS             complement(<3733..3968)
                     /locus_tag="PKL11_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNZ13816.1"
                     /translation="MTPRARLVVLISGHGSNLQAILDACASGELNAAVVAVISNRSEA
                     FGLERARQIGVPALVLPKLKDQDRNTYDARLADLV"
     gene            complement(3965..>5136)
                     /gene="purM"
                     /locus_tag="PKL11_09195"
     CDS             complement(3965..>5136)
                     /gene="purM"
                     /locus_tag="PKL11_09195"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNZ13817.1"
                     /translation="RLLTAGGRVLGVTAWDTDLPAALDRAYQAVDRITFEGAQYRHDI
                     GSAHLKGAGAYASAGVNIDAGNEAVRRMSAAVKSTYTPDVLAGIGAFGGLFDATRLKS
                     MNSPVLVASTDGVGTKVKLAAALNSYASIGQDIVNHCIDDILVQGASPLFFLDYFATA
                     RLDPAVVAEIVGGMSQACGESNCVLIGGETAEMPGVYAAGEFDVAGTIVGLVERAAIL
                     PRPELCAGDLLVGLRSSGPHTNGYSLIRHIFADADYTAFYPELNCTLADALLAPHRSY
                     LPVLNPVLAHPERPIKALAHITGGGFIENIPRVLPDGISAHIRWDSWPIPPLFSLIQQ
                     RGQVGWAEMARVFNLGIGMVAVINPEHLSLFQQLVGEETWVIGSLTYDTRRVVML"
BASE COUNT         1169 a         1367 c         1523 g          977 t
ORIGIN      
        1 gtaatatgtg ctttaacaga aggggcgccc tgatgggtcg tttttgtatt ccaagctgag
       61 ccaaagggct cccctattta attatgaaaa aatgcggttc acattttgat gtgaaccgta
      121 attttcaaca gcacttgagg agaaatcagt cagagcggcg gtagttgggc gcctcacggg
      181 tgatgaccac atcgtgggga tggctttcga tcaggcccgc gttggttata cgcactagct
      241 gcgtgtgagt acgcaggtct gtcagcgagg ctgcaccagc gtaacccatg ccagagcgca
      301 aaccgcccac aagctgataa acaaaatctg cgagggtgcc tttgtacggc accataccct
      361 caaccccttc aggaaccagc ttgcccgcct taccctgccc ggtgccgtaa cgatcacgac
      421 cgtagccctg catggcgccc aggctgccca tgccgcgata ctgcttatac tggcggcctt
      481 catagacaat cacttcgcct ggggcttctt caagaccagc caacagactg cccagcatga
      541 ccacatcggc accagcgaca atggctttga caatatcgcc gctgtatttg acgccgccat
      601 ctgcgaccac cgtgacaccc tgacggtgtg caacctgggc gcaggcgtga atggcagtca
      661 attgcggcat gcccgcgcca gcgataatac gggtggtgca aattgagcca gcccccacgc
      721 cgaccttaat gacatctgca ccagcatcaa tcaaagcctg ggtgccttca gcggtcacga
      781 cattgccggc aattactgga aggtgcggat agactgattt gattcgctgg atcgtacgga
      841 taacgcccgc agtatgacca tgggcagtat ccaccacaac aacatccaac ccacaggccg
      901 tcatccgttc gatgcgatct tcaacatcgg ggccaacgcc cactgcggcg ccaacgcgca
      961 agcgcccacg cgaatcgatg ctggcgttgg ggtattcgat ttttttcaag atgtctttta
     1021 cggtaatcag gccggttaag cgtccggcag gatcgaccag ggggagtttt tcaatgcggt
     1081 gctgctgcaa aatggcctga gcctgctcca gagtggtgtg ggcaggggcc gtgaccaggc
     1141 cagtagcggt cataaagtcg ctgacagggc ggacataatc ttcagggccg gtgaagcgga
     1201 tatcgcggtt ggtcagaatg ccgacgagac gctgagtctg ggggtccaca attggcacac
     1261 cgctgatatg gaagcgcgac atgatttctt cggcctcctg cagggtggcg gtttgcggca
     1321 gggtgatggg atcgacaatc atgccggact cagaacgctt gacggtctca acttctttgg
     1381 cttgagcctc gggcgagagg ttgcggtgaa tgatgcccaa cccaccctcg cgcgccagag
     1441 ctatcgccag ccgggcttcg gtgactgtat ccatggcggc ggagagcaac ggggtattga
     1501 gctggattgc atcggtcaac cgggcacgga tatcggtatc ggtagggagc acctggctga
     1561 aaccggggac gatcaagaca tcgtcaaagg tcaaagcctc tcgagcggta aacagatctt
     1621 cattcattgc aatctcctgg ggtgatattc ttcggttgcg acgggcgaaa gctgactaga
     1681 agcatcagcg tggattatac ctgcctaatg gcgaaaatgg cggacgccgg tggttaccat
     1741 ggcaagacca gccgcatcgg cggcagcaat gctttcagcg tctcgcacag agccgccggg
     1801 gtgaacgatt gctgtaatgc cagcctggat ggcaacttcg acagaatcgg ggaatgggaa
     1861 gaaggcgtca gaggccatga cggcgccgcg ggagcgctcg ccagaacgct gggcagccat
     1921 gcggacgcag tccacccggt tgggctgacc gccgccaatg ccaaccgtgg cctcgccctg
     1981 ggcaaagaca atggcattcg atttgacatg ctgagcggct ttccaggcaa agcggagaga
     2041 gcgccactcg gcttcggtgg gttgacgctg ggaaacgacc gtccattggg cagcaaatgg
     2101 gtcgccaagg tcaatggact gctgcagcaa gccgcggtga atggagcggt attcgaccga
     2161 tggatctacg ggtgtattgg gcacaaccag aagacgcaga tttttcttct tcgagaagat
     2221 ttcaagcgcc tgagggtcaa aagccggggc aacaacacat tccacgaaca ggtctttgat
     2281 ggcctcggca gtttcgccgt ccagcgggcg gttggaggca acgatgccgc caaaagccga
     2341 gatgggatcg ctggcaagcg ccaggcggta ggcatctaca agcttgtcag cagaggccat
     2401 gccgcaaggc gagagatgtt tgacaatgac aatggagggc gattcaaaag cgcgaaccgc
     2461 acgccaggag gcatcaagat caaggaggtt attatatgaa agctctttac cctgcaagac
     2521 ctgcccgcca agcggacctt ctccgggatg atgcgaaaat agggtggctt gttggtgcgg
     2581 gttctcacca tagcgtaggg tttgcaccgg gtagagctgc aagtttagtg cagaggagcc
     2641 ggagaagtag gcggtaatcg ccgagtcgta gtgggcggtt tgtgtaaagc ccttgaccgc
     2701 caggttacgg cgcatgtctg cgggaatgcc gccggcgcgc agcgcagcga gcgcggcagg
     2761 gtaatccgcc gggtcgcaga tcagtgtgac tcgctggtgg tttttagcgg cggcgcggat
     2821 cagggtaaca ccgccgatat caatattttc aattgcttcc gccaaggtaa catcaggacg
     2881 ggcaatggtc tgttcaaaag gatagagatt gactgcaacc agatcgatgt aatcccagcc
     2941 aagattctca agctcatgat gatcagcctc tgtggcgcgt gccaggaggc cgccgtgaat
     3001 ggcggggtgg agggtcttca cgcggccgcc caaaatttcg ggagagccag tatagtctgc
     3061 gacttcggtc acgggtagat tgtggtcgtg cagcatccgc gccgtgccac ccgaggcaat
     3121 taactgccat cccagggcgt gcaaaccgga ggcaaattcg accaggccgg ttttatcatg
     3181 aacagacagt aaagctttgg gcattttttg gatatccttt cactatttct gaatcaattg
     3241 ttgaagggtt tgcaccagca gttgatgttc agcctgatgc acccgctcgc tcaaggtttc
     3301 caaagtatcc tgaggagtaa tgggcagttc gatctgtgca agcacgggcc cagcatccac
     3361 tccctcgtct ggcaccagat gggtcataac tccggtagat tggatttcgc cgcgctggta
     3421 agcctcaaaa gctcgggcga tggcctctgt gcccgggaaa gcaccgggca gagccgggtg
     3481 cagattcacc acccggttgg ggaaatggga cagaaaggac atggagagca gccgcatcca
     3541 ccccgccaat ataacccaat cgggctgata agacagcacc aggtcggcga gccgggcatc
     3601 ataggtgtta cgatcctggt ctttcagttt ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnaccaggtc ggcgagccgg gcatcatagg tgttacgatc ctggtctttc
     3781 agtttgggta gcaccagggc aggcacgcct atctggcgag cgcgctccag gccaaaggcc
     3841 tcggagcgat tggaaatgac agccaccaca gcagcattca actcgcccga ggcgcaggca
     3901 tccaggatag cctgcaaatt agagccatgc cccgagatca aaacaaccag acgggctctg
     3961 ggggtcataa catcaccacg cgtcgcgtat cataggtcaa agacccgatg acccaggttt
     4021 cttctccaac aagctgctgg aaaagcgaaa gatgttcggg gttgatgacg gcgaccatcc
     4081 caatgccgag attgaagacc cgcgccattt cggcccagcc cacctgaccg cgctgctgaa
     4141 tcaaactgaa aagtggcgga ataggccagc tatcccaacg aatgtgcgcc gaaatcccat
     4201 ccggcaggac gcgggggatg ttctcaataa agccaccgcc ggtgatatgt gccagagcct
     4261 tgatggggcg ttcgggatga gcgaggacgg ggttgagcac tggcagatag gagcggtgcg
     4321 gggctagcaa agcatctgcc agggtacagt taagttccgg ataaaatgct gtgtaatcgg
     4381 catcggcaaa aatatggcgg atcaaagagt atccattggt atgcgggccg gatgagcgca
     4441 gcccgaccag caaatcgcca gcgcaaagct cggggcgggg cagaatggcg gcgcgttcga
     4501 ccaggccgac aatggtgcca gcgacatcaa attctcctgc agcgtaaacg ccgggcattt
     4561 cggcggtttc gcccccgata aggacgcaat ttgattcccc acaagcctga gacatgccac
     4621 cgacaatttc ggccactacg gcagggtcaa gccgggcagt ggcaaaataa tccaggaaga
     4681 acaggggcga ggccccctga accagaatat catcaatgca atgattgaca atgtcctgtc
     4741 cgatgctggc atagctgttg agcgccgccg ccaatttaac cttggtgccg acgccatctg
     4801 ttgaggcaac cagaaccggg ctgttcatgg atttgaggcg ggtggcatca aacaaaccgc
     4861 caaaggcacc aattccagcc aagacatcgg gagtgtaggt tgatttgact gccgcgctca
     4921 tgcggcggac cgcttcgttg ccggcatcga tattgacgcc ggcgctggca taagcgccag
     4981 cccccttcag gtgtgctgaa ccaatatcat ggcgatactg cgctccctca aaagtgatgc
     5041 ggtccacggc ttgataggct cgatccagcg cggcaggaag gtcggtatcc caggcggtaa
     5101 cgccaaggac acgcccgccc gcggtgagca agcggt
//
LOCUS       DAOPVS010000136         5121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16327_length_5121_cov_51.486775, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000136 DAOPVS010000000
VERSION     DAOPVS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16327_length_5121_cov_51.486775"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1414
                     /locus_tag="PKL11_09200"
     CDS             155..1414
                     /locus_tag="PKL11_09200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNZ13818.1"
                     /translation="MNARRRKTSWLIWVGIVLGLGLVALLGWANYRYTSENPGGNDFV
                     VHWIGARSFLYDGLSPYSDETSERIQRFVYGRPAQQGEHELRVAYPLYSVALFLPFAL
                     IKDFVLARAIWMTVLEIGLAATALLSIRLARWRPGLPVLILFMLFSLVWYHAVRPLIN
                     GNAVILVVLVLVGALLAIRSRQDEIAGILLALTTIKPQLVVLVIAFIAIWAIAQKRWK
                     IISWLAASVFILSAFAALFLPDWIVQNLREVLRYPGYNPPGTVAAAFAEFWPGFGKTL
                     GWIFSGFFIIVMLVEWWLHRKAEFRGFLWTVCLTLALSGWVGIQNDPGNFIMMAPALA
                     LIFAGLDARWKRASLPVTLGSLILLLVAIWAIFFATVEYTYQPIQSPVMFFPLPAFLF
                     IMLYWVRWWAVRPPNTWFEQITADENL"
     gene            1479..1925
                     /locus_tag="PKL11_09205"
     CDS             1479..1925
                     /locus_tag="PKL11_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNZ13819.1"
                     /translation="MKKLGRKIGLVILVLLGLLGIIQLVPYGRQHTNPPVLSEPQWDS
                     SDTRILAQRACFDCHSNEVIWPWYSSIAPGSWLIQHDVEEGRSKLNFSEWQQGGNENF
                     DEIQEVLMAGEMPPIQYLLLHPVARLTEAEKKQFLQGLQNSIGAGR"
     assembly_gap    2089..2188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2189..3607
                     /locus_tag="PKL11_09210"
     CDS             <2189..3607
                     /locus_tag="PKL11_09210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ13820.1"
                     /translation="RLIVIGTRSIQYDDAFSIFLSERSLAEIVRGTAADTMPPLYYFL
                     LHFWLQVGRSLTWLRLLSVLLSLGSVTVLYLLVRRLFGHSAGIWAALLAAISPLQLYH
                     AQDLRMYALLALSQLSYYYFFIRIWRPGERGTRWTDWLGLVLCGAAAMYSHNLAVFGL
                     VIPNLVLLFRREWKPLLRLLVAQAAMGLLALPWLWLVPGQVAKIQRAFWTPRPGLIEV
                     MQAILLFVIHLPLPGVWMMIGGVIAAQAYVLTLLETWRARREGWGLALLIGVMLGLPI
                     LLFITSYLIRPVFVTRGFLVASLAFYALAGRAVAKGWRRGIGPFLLGLFVIGSVISLP
                     AYYSFESFPRSPFQAAVTALQAQIQPGDVILHDNKLSYFPSHYYAPNLPQVFLADEPG
                     SSNDTYALASQEAMQLFPQPDVTTAIATAPRIHFIVFTQAIRDYQSMGISTHPVLAEL
                     NACCSLVAHQTFGDLEIYTFDR"
     gene            3626..>5121
                     /locus_tag="PKL11_09215"
     CDS             3626..>5121
                     /locus_tag="PKL11_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13821.1"
                     /translation="MPVPPVRSQSIAFRLWPILLVINLAVIAVCWQFQRSPGYMDSAY
                     YYIGARQLASGQGLSEPILWNYLDDPAGLPHPSHTYWMPLTSLVSSLGMLLFKNTGFL
                     AARIPLSLLAGLLPWLVAALAARLDPAASPAQRSRRWLLAWVLAAFSGFYLQYFTLTD
                     SFILYMLLGTGFLWLGFGLQSTAAEPAPSGKGLWLRFLLLGVLAGLLHLTRADGLLWL
                     LAAPLAAAWAVGRDFYRQPRFYGLLLLIVLGYLLTAGWWLSRNLQLYGTPLPGGSQTL
                     WLTTYDDTYAYPAGSLTFENWWAAGLGAALSARWTAFLWNLANLVGVQGLVILLPLML
                     IGLWRLRSRRLVQAAAALWLLTFAVMTLVFPFAGSRGGYLHSGAAFQPLLWAAVPLGL
                     DAVIDWGARHRNWKRSQAWSVFGVGIGLLAVALTVGLYATRIIGPQPLTPLWDADERT
                     FQQVDLALVSLGAPADAVIMVNNPPGFNAATRRPAVVIPNGDAATALAA"
BASE COUNT          801 a         1534 c         1315 g         1371 t
ORIGIN      
        1 tgaggacatt ccctcttttt gtcaaccgtt ttttgaattc acatcagacg tattgtatta
       61 cctcaattca tgccagccgt ctcaatcttc gtttatcaaa tcctattata attgacctga
      121 ggggcattcc gcgccagacc acaggagtag tcatttgaac gctcgtcgca gaaaaacatc
      181 atggttaatt tgggtcggaa ttgtgcttgg cctcgggctg gttgcattgc tgggctgggc
      241 caactaccgc tacacctcag agaaccccgg cggcaatgat tttgtcgttc attggattgg
      301 cgcccgctca ttcctgtatg atggcctcag cccctactca gacgaaacct ccgagcgcat
      361 ccagcgcttt gtctatggtc gcccggccca gcagggggaa catgagctgc gtgttgcata
      421 tccgctctac tcggtagccc tcttcctgcc ctttgccctg atcaaagatt ttgtcctggc
      481 ccgcgccatt tggatgaccg ttctcgaaat cggcctggct gccacagcgt tgctctcaat
      541 tcgcctggcc cgctggcggc ccggcctgcc cgtgctgatc ctcttcatgc ttttctcgct
      601 ggtctggtat cacgcggtgc gtccgttgat taatggcaat gccgtgatcc tggttgtgct
      661 ggttctggta ggtgccttgc tggccatccg ttcccgtcag gatgaaattg ccggtattct
      721 cctggcgctg acgaccatca agccccaact ggtggttctg gtcattgcct tcattgcgat
      781 ttgggccatt gcccaaaaac gctggaaaat tattagctgg ctggctgcca gtgtcttcat
      841 cctgtcagcc tttgccgcct tattcttgcc cgactggatt gtgcaaaatc tgcgtgaagt
      901 cttacgctac cccggatata accctcccgg cactgtcgcg gctgctttcg ctgaattttg
      961 gcccggcttt ggcaaaaccc tcggttggat tttctctggt ttctttatca ttgtcatgct
     1021 ggttgagtgg tggctgcatc gcaaagccga gtttcgcggc tttctgtgga cagtgtgcct
     1081 gaccctggct ctgagcggct gggtaggcat tcaaaatgac ccgggcaact tcatcatgat
     1141 ggccccggcc cttgcgttga tttttgccgg tctggatgcc cgctggaagc gcgccagcct
     1201 gccggtcacg ctgggcagcc tcattctgct gctggttgcc atctgggcta ttttcttcgc
     1261 cacggtggag tacacctatc aacccatcca gagcccggtg atgttctttc cgttgccggc
     1321 ttttttgttc atcatgctct attgggtgcg ctggtgggca gtgcgccccc ccaatacttg
     1381 gtttgagcaa atcactgcag atgaaaatct gtgatttgat tgccaactta ccctttgaaa
     1441 tttgacatac tctctatcga tgtattctgg aggtaacaat gaaaaaactt ggacgtaaaa
     1501 ttgggttagt tattcttgtg ctgcttggct tattggggat catccaacta gttccttatg
     1561 gccgtcaaca taccaatccg ccggtccttt ctgaacctca atgggatagt tctgacacgc
     1621 gaattttggc tcaacgggcc tgctttgatt gtcacagcaa cgaagtgatc tggccttggt
     1681 atagcagcat tgcacctggt tcatggctta ttcagcatga tgtagaagaa ggtcgtagta
     1741 aattgaattt ttcggaatgg cagcaaggcg gaaacgagaa ttttgatgag atccaagaag
     1801 tattgatggc aggggagatg ccccctatcc agtatttatt attgcatcct gttgcccgcc
     1861 tgaccgaagc tgaaaaaaaa cagtttttgc aaggtcttca gaattcaatt ggtgctgggc
     1921 gttaatttac ttcactacat cggacggttt taaatcgata agcaatcaat aaaaatatga
     1981 tgcgacgcac tggttggcag ttttggggaa tattggtgct ggctctggta ttacgcttga
     2041 tagtgatcgg cacgcgcagc attcaatatg atgatgcctt cagcatctnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cttgatagtg atcggcacgc gcagcattca
     2221 atatgatgat gccttcagca tcttcctgtc tgaacgcagc cttgcggaaa ttgtccgcgg
     2281 caccgctgcc gataccatgc cgccgctgta ctacttcttg ctgcatttct ggctgcaggt
     2341 tgggcgctcg ctgacgtggc tgcgcctgtt gagcgtgctg ctcagcctcg gcagcgtaac
     2401 cgtgctttac ctgctggttc gccgcctgtt cggtcattcc gccggcatct gggcggcgct
     2461 gctggctgcc atttcacccc tccagctata ccacgcccag gatttgcgca tgtatgccct
     2521 gctggccctc agccagttga gctattacta cttcttcatc cgcatctggc gcccgggtga
     2581 acgcggcacc cgctggaccg attggctggg cctggtgctt tgcggcgccg ctgcaatgta
     2641 ttcgcataac cttgcggtct ttggtctggt cattcccaac cttgtcctgc ttttccgccg
     2701 cgaatggaag cccctccttc ggctgttggt cgctcaggcc gccatgggtc tgctggccct
     2761 gccctggctc tggctggttc ccggtcaggt tgccaaaatc cagcgtgcct tctggacgcc
     2821 tcgcccgggc ctgatcgaag tcatgcaggc gattctgctc ttcgtgatcc atctaccgct
     2881 ccccggtgtc tggatgatga ttggcggcgt gattgccgcc caggcctatg tgctgacctt
     2941 gctggaaacc tggcgcgccc gccgggaagg gtgggggttg gcgctgttga ttggtgtgat
     3001 gctgggcctg cccatcctgc tctttatcac ctcgtatctc attcgcccgg tcttcgtcac
     3061 ccgcgggttt ttggtggcat ctctggcttt ctatgccctt gccggccgcg ctgttgccaa
     3121 aggctggcgg cgcgggattg gccccttctt gctgggcctg tttgtcattg gctcggtgat
     3181 ttcccttccc gcatactact cgtttgaatc cttcccccgc tccccattcc aggcggctgt
     3241 cactgcgctc caggcccaga ttcaacccgg tgatgtcatc ctgcacgata ataagctcag
     3301 ctacttcccg tctcactact acgccccaaa tttaccccag gtcttcctcg cagatgagcc
     3361 cggctcctcg aatgatacct atgccttagc ctctcaggag gccatgcagc tcttccccca
     3421 gcccgatgtg accaccgcga ttgcaacggc ccctcgcatc cattttatcg tcttcaccca
     3481 ggccattcgt gactatcaat ctatgggcat ttcaacccac cccgtgttgg cagaattgaa
     3541 cgcctgctgt tctctggtcg cccatcaaac ctttggtgat ctggaaatct acacttttga
     3601 ccggtaacct ctgatgaata ctggcgtgcc cgttcctcct gttcgttcgc agtccattgc
     3661 ctttcgtctc tggccaatcc tgctggtgat caacctggct gtcattgcag tctgctggca
     3721 gtttcaacgc tcccccggct acatggattc ggcctactat tatattggcg ctcgccagct
     3781 cgcttccggt cagggcctct ccgagcccat cctctggaat tatcttgatg atcccgccgg
     3841 tctgccccat ccctcacaca cctactggat gcccctcaca tccctggtca gcagtcttgg
     3901 aatgctgctt ttcaaaaata ccggcttcct ggcggctcgc atcccgttga gccttctggc
     3961 tggcctcttg ccctggctgg ttgccgccct ggccgcccgc ctggatcctg ccgccagccc
     4021 tgctcagcgt tcccgccgct ggctgctggc ctgggtgctg gctgcctttt ctggtttcta
     4081 cctccaatac ttcactctca cagacagctt catcctttac atgctgctgg gcacaggctt
     4141 cctctggctg ggctttggcc tgcaatccac tgccgccgag cctgcgccat ccggcaaagg
     4201 actctggctg cgctttttgc tgctgggcgt cctggccggt ctcctgcacc tcacccgcgc
     4261 cgatggcttg ctctggctcc tggccgcgcc gttagcggct gcctgggctg tcgggcgtga
     4321 tttttatcgc caaccccgct tctatggttt gttgctgctg attgtcctgg gctacctttt
     4381 aacggcaggt tggtggctgt cccgcaacct tcagctctat ggcacgcctt tgcccggcgg
     4441 ctcgcaaacc ctctggctca caacgtatga tgatacctac gcttacccgg caggctccct
     4501 cacgtttgaa aactggtggg ctgccgggtt gggcgccgcc ctgtctgccc gttggacggc
     4561 cttcctctgg aaccttgcca atctggtggg ggtgcagggg ttggtcatcc tcctcccgct
     4621 catgctcatc ggcctctggc ggctgcgctc ccgccgcttg gtccaggctg ccgccgcgct
     4681 ctggctgttg acctttgccg tgatgacact tgtcttccca tttgcgggct cgcgcggcgg
     4741 ctatctgcac tccggcgcag ctttccagcc cttgttatgg gccgctgttc cgctggggtt
     4801 agacgcggtg attgattggg gcgcgcgtca ccgcaattgg aagcgtagtc aggcctggtc
     4861 agttttcggt gtgggcattg gattgctggc agtggcgctg accgttggcc tgtacgccac
     4921 ccggatcatt ggtcctcagc ccctcacccc cctttgggat gccgatgaac gcaccttcca
     4981 gcaggtagat ttggccctgg tatccctcgg cgcccccgcc gatgcggtga tcatggtcaa
     5041 taacccaccc ggcttcaacg ctgccacccg ccgcccggca gttgtcatcc ccaatggcga
     5101 tgccgccacg gcccttgccg c
//
LOCUS       DAOPVS010000137         5111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16370_length_5111_cov_59.190269, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000137 DAOPVS010000000
VERSION     DAOPVS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16370_length_5111_cov_59.190269"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1227)
                     /locus_tag="PKL11_09220"
     CDS             complement(1..1227)
                     /locus_tag="PKL11_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HNZ13822.1"
                     /translation="MNPWVTLIIVAITYLGIAIGHWPRLKTNRTTIALMGAGLLLVTG
                     QIKFDQIGSFLDLDTLILLFSMMIINANLRLAGFFELAANRLLRLTRNPRHFLALEIL
                     LMGVLSAFFLNDTICLMFTPFLLELMLAARRNPIPYLIALATAANIGSAATLTGNPQN
                     MIIGVASGISWLQFAAALTPLALLSLGGIWGVLILFYPQEFARRNLFDILPATEVRYY
                     PWHLTKCLIIIGGLLLAFLLGAPVAFAAFLAACALLFTRRVQPESVFAEFDWSLLVFF
                     AALFIVTGSLEVSGLTNRLLELTHLNEQVNVFSFSAITVLLSNLVSNVPAVLLLRPLT
                     ASLPNPTAGWLTLASASTLAGNLTLLGSVANLIVAESALRRDVKLTFWEYTRTGALIT
                     LISLLISTLWIALFIW"
     gene            1323..>2146
                     /locus_tag="PKL11_09225"
     CDS             1323..>2146
                     /locus_tag="PKL11_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNZ13823.1"
                     /translation="MKTRFRLILTGLTALALLLSACTVPGGQNLGEVRSTHQRVADPQ
                     VPTEDLAALTAGERAFALDLYQQLRTTEGNLFYSPYSITLALAMTYAGARNQTETEMA
                     QTMHYDLAQTALHPAFNGLDQALMSRAENIPAEQGDGFTLSVANALWGQVDYAFQAEF
                     LDLLAENYGAGMQTVDYANDAEGARQLINQWVADETRDKIKDLLQPGAVDALTRLVLT
                     NAIYFKAGWINTFDETLTAEAPFYKLDGSTANIPMMHVNEGFGYLAGDGFQAIELP"
     assembly_gap    2147..2246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2247..2698)
                     /locus_tag="PKL11_09230"
     CDS             complement(<2247..2698)
                     /locus_tag="PKL11_09230"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNZ13824.1"
                     /translation="MNRKIIIPLIVLALVSMACSFTVNVPTIKTGETQTTTLQEAIPT
                     GGTDPMRLEIEMGAGELDIRAGAQAFLEGTIECNVDAWQPEIRRGSYSVEVSQQSTGN
                     VSVPEDKLVNEWDLRLGNYPTDLSIKAGAYQGEIDLTGLPIVDLEITDG"
     gene            complement(2762..3931)
                     /locus_tag="PKL11_09235"
     CDS             complement(2762..3931)
                     /locus_tag="PKL11_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNZ13825.1"
                     /translation="MNKFVKWMFVISLLVTALALGFSPVQAAGPASDCPPNSPVIGSA
                     CRISNGTVYEGDLAIMGGTVTIEDGAIVEGDVALMGGTLDVEGVVEGDIAAFGGMLTL
                     DSTAVVEGNIAANGVQFNRDPDAVVGGDVVLNSASAESWSEVVPWNWDWESRYNESPY
                     FHFMNGVNSLLWNLFQVLGVAALALVLALLMPAPVERLGRAVSRQPVTSGGIGLLAFI
                     AIPAALVLMCITIILIPVALLSLPVIAIAVLIGWIGVGLELGNRLAAVFKTTWATPLA
                     AAIGTLLVSFVSALLALIPCVGWLFGFIIALVGLGAVITTRFGSREATLDPIRGSTPP
                     AAPAPVVIPEPSPAYIPEEPVVVEPETPAEEPAPARKRPARKVAAADEPPVEPGQ"
     gene            complement(3928..4476)
                     /locus_tag="PKL11_09240"
     CDS             complement(3928..4476)
                     /locus_tag="PKL11_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13826.1"
                     /translation="MSHQPFETLIYQDVPRTTQQEAELQLHLKDCPQCSRNEKAWMDV
                     RCQLAAAPMARPAPGFTKRWEAKFAVERARQRERQIWLTILIIVGSLLLILALIGLII
                     SLNATPASLLAGAIAAFVNFSNWLVQAQQTVSQLLAHTPTWVWWGLGALVLVWFSVVS
                     LTGAFAFCRATRLCNFNRGVQS"
     gene            complement(4457..5050)
                     /locus_tag="PKL11_09245"
     CDS             complement(4457..5050)
                     /locus_tag="PKL11_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ13827.1"
                     /translation="MTYNESDCLQQALQGSDEAFTALVERYQTPVYNLCYRMLGSPQE
                     AEDAAQETFWRAYQHLHRYDPNRSFATWLLSIAAHYCIDQQRRSRAVLIPLDGMIEEI
                     MADPAPLPERSAARSQEATALRQLLNSLNPQDRAAIVLRYWYDFSEEEIANALALTVS
                     AVKSRLHRARQALGQRWQALEAQNAAQERRPDESPAF"
BASE COUNT         1086 a         1351 c         1677 g          897 t
ORIGIN      
        1 ttaccagatg aacagggcaa tccagagggt gctgatgagc agggagatca gggtgatgag
       61 ggcgccggtg cgggtgtatt cccagaaggt gagtttgaca tcacggcgca gggcggattc
      121 ggcgacaatc aggttagcca cggagcccag cagcgtgagg ttgcccgcca gggtggaggc
      181 ggaagccagg gtgagccagc cggcggtggg gttgggcagg ctggcggtca gcgggcgcag
      241 taacaggacc gccggaacgt tggaaaccag gttgctgagc aggacggtga tggccgaaaa
      301 gctgaagaca ttgacctgtt cgttcaggtg tgtcagttcc agcaggcggt tggtcagccc
      361 gctgacctcg agggagccgg tgacgataaa gagggcggcg aagaagacca acaggctcca
      421 gtcaaattcg gcaaagaccg actcaggctg gacgcggcgg gtgaagagca gcgcacaggc
      481 agccaggaag gccgcaaaag ccaccggcgc gcccagcaaa aatgccagca gcaggccgcc
      541 gatgatgatc aggcacttgg tgaggtgcca gggatagtac cgcacctcgg tggcgggcag
      601 aatatcaaag aggttgcggc gggcaaattc ctgcgggtag aataaaatca gaacgcccca
      661 gatgccgccc aggctgagca gcgccagcgg ggtgagcgcg gcggcaaact ggagccagga
      721 gatgcccgag gcaaccccga taatcatgtt ctgcgggttg ccggtgaggg tggcggccga
      781 gccgatattg gcggccgttg ccagggcaat cagatagggg atggggttgc ggcgcgcggc
      841 cagcatcagt tccagcagga agggagtgaa catcaggcag atggtatcgt tcaggaagaa
      901 ggcgctgagc acgcccatca gcaggatctc gagggcgagg aagtggcgcg ggttgcgcgt
      961 gaggcgcaga aggcggttgg cggccagctc aaaaaagccg gccaggcgca ggttggcgtt
     1021 gatgatcatc atgctgaaga gcaggatgag ggtatctaaa tcgaggaagc tgccgatctg
     1081 gtcaaatttt atctgcccgg tgaccagcag gagcccggcg cccatcaggg cgatggtggt
     1141 gcggttggtc ttcagccggg gccaatgccc gatggcaatc ccgagatagg tgatggcgac
     1201 aatgatcagt gtaacccacg gattcatgga tgcaattata caaccctgga accgaatgag
     1261 gggaaacagc gtcttaaatt cactctgcaa agagattgat tttgtttgac tggaggatga
     1321 cgatgaaaac aagatttcga ttaatactca ccgggttgac cgcactggca ctgctgcttt
     1381 cagcctgcac cgtgccgggc gggcaaaacc tgggcgaagt ccgctccacc catcaacgcg
     1441 tggcagaccc acaggtgccg accgaagacc tggcagccct gaccgccggg gaacgggcgt
     1501 ttgcgctgga cctgtaccag cagctgcgga ccactgaggg caatctgttt tactcgcctt
     1561 acagcatcac cctggcgctg gcgatgacct atgccggggc gcgcaatcag accgagacgg
     1621 agatggcgca aacgatgcac tacgatctgg ctcaaacagc gctgcacccg gctttcaacg
     1681 ggctggacca ggcgctgatg agccgggcgg aaaatatccc ggcagagcag ggcgatggct
     1741 ttacgctgag cgtggctaat gcgctgtggg gccaggtgga ctacgccttc caggctgagt
     1801 ttttggatct gctggctgaa aattacggcg cgggcatgca gacagtagac tatgcgaatg
     1861 acgctgaagg cgcccgccag ctcatcaatc agtgggttgc cgatgagact cgcgacaaaa
     1921 tcaaagacct gctgcagccc ggcgcagtgg atgcactgac ccggctggtg ctgaccaatg
     1981 caatttactt caaggcgggc tggatcaata cctttgatga gaccctgacc gccgaggcgc
     2041 ccttctataa gctggatggc agcactgcca atattccaat gatgcatgtt aatgaagggt
     2101 ttggctatct ggcaggagac ggcttccagg cgattgaact gccctannnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnccat cggtaatttc caggtccaca atcggcagac
     2281 cggtgaggtc aatctcgccc tgataggcgc cggctttaat ggacagatcg gtggggtagt
     2341 tgcccaggcg caggtcccat tcattgacca gtttatcttc ggggacggag acattgccgg
     2401 tgctctgttg agagacctca accgagtagg agccgcggcg aatttcgggc tgccaggcat
     2461 ccacattaca ttcaatggtg ccttccagga aagcctgggc gcctgcgcgg atatctaact
     2521 ccccggcgcc catttcaatt tccaggcgca tggggtcagt gccgccggtg gggatggctt
     2581 cctgcagggt ggtggtctgg gtttcgccgg tcttgattgt ggggacgttg acggtgaagg
     2641 aacaggccat ggagaccagc gccaggacaa tcagtgggat gatgattttg cgattcatgg
     2701 gtaaatcctc cagaaatctg cggcaggggg ttgccctgcc gcagtatttg atgattgggt
     2761 tctactgacc cggttcaacc ggcggctcgt cggcagcagc caccttgcga gcggggcgct
     2821 tgcgggcggg agcgggttct tcggcgggtg tttcaggctc aaccacaacg ggttcttcgg
     2881 gaatatacgc cggggatggt tcggggatga ccacgggcgc cggggcagcc ggaggcgtgg
     2941 agccgcggat ggggtcgagg gtggcttcac gcgagccaaa gcgggtggtg atgacagcac
     3001 ccaggcccac cagggcgatg atgaagccga acagccagcc cacgcagggg atgagcgcca
     3061 gaagcgccga gacaaagctg accagcaggg tgccaatggc cgcggcaagc ggggtggccc
     3121 aggtggtctt gaagacagcc gccaggcggt tacccagctc caggccgaca ccaatccagc
     3181 caatcaggac agcaatggca atcacgggca gggagagcag tgccacgggg atcaggatga
     3241 tggtgatgca catcagcacc agggccgcgg ggatggcgat gaaggccagt aagccaatgc
     3301 cgccgctggt gaccggctgc cgggagacag cccgacccag gcgctccacc ggagccggca
     3361 tgagcagcgc caggaccaac gccagggcag ccacgcccag cacctggaag agattccaga
     3421 gcaggctgtt gacaccgttc ataaagtgga agtagggcga ttcgttgtag cggctttccc
     3481 aatcccagtt ccaggggacg acttcgctcc agctttcagc gctggcgctg ttcaagacga
     3541 catccccacc cactacggca tcggggtcac ggttgaactg cacgccgttg gcagcgatat
     3601 tgccctcgac cacggctgtg ctgtcgagag tgagcatacc gccaaaggcg gcaatatcgc
     3661 cttcgaccac accttcaaca tcgagggtgc cgcccatcag ggccacatcc ccttcgacaa
     3721 tggcgccgtc ttcaatggtg acggtgccgc ccatgattgc caggtcgcct tcatagacgg
     3781 tgccgttgga aatgcggcag gctgagccga tgacgggcga attggggggg cagtcactgg
     3841 cggggccagc cgcctgaacc gggctgaagc cgagcgccag cgcggtgacg agcagactaa
     3901 tgacaaacat ccattttacg aacttattca tgattgaact cctcgattga aattacacag
     3961 gcgggttgcc cggcaaaagg caaatgcgcc cgtaaggctg acaactgaga accagaccaa
     4021 aaccagggca cccaggcccc accagaccca cgtgggggta tgggccagca gttggctgac
     4081 tgtttgctgg gcttgaacca gccagttgga aaagttgaca aaggccgcaa tggccccggc
     4141 taacaaactg gccggggtgg cattgaggct gataatcagg ccaatcaggg ccaggatgag
     4201 cagcaggctg ccaacgataa tcaggatggt cagccagatc tggcgctccc gctgccgggc
     4261 gcgctccacg gcaaatttag cttcccagcg cttggtgaat ccgggggccg ggcgcgccat
     4321 gggggcggca gccaactggc agcggacatc catccaggct ttttcattgc ggctgcactg
     4381 cgggcaatcc ttgaggtgga gctgcagttc agcttcctgc tgggtggtgc ggggaacatc
     4441 ctgatagatt agggtttcaa aaggctggtg actcatcggg tctcctttcc tgggcggcgt
     4501 tttgcgcttc cagcgcctgc cagcgctgcc ccagggcctg acgggcccgg tgcagccggc
     4561 ttttgacggc actgacggtg agcgcaagcg cattcgcgat ttcttcttct gaaaagtcat
     4621 accaataacg gaggacaatg gcggcgcggt cttgcgggtt gaggctgtta aggagctggc
     4681 gcagagccgt ggcctcctgc gatctggcag cggagcgctc gggcaggggt gcggggtccg
     4741 ccatgatctc ttcgatcatg ccatccaggg ggatgagcac ggcacgcgag cggcgctgct
     4801 ggtcaatgca atagtgagcg gcaatggaca ggagccaggt ggcaaaggaa cgattgggat
     4861 cgtagcggtg aaggtgttga taggctcgcc agaaggtttc ttgagcggca tcttcggctt
     4921 cctgcgggct gcccaacatg cggtagcaaa ggttatagac gggcgtttga taacgttcca
     4981 ccagggcggt gaacgcctca tcactgccct gcagggcttg ctgtaaacaa tcactttcgt
     5041 tgtatgtcat ccgatttcct gttccagcgc ttacagtaca gtatacgcaa gttttggcgc
     5101 aaggttgcac c
//
LOCUS       DAOPVS010000138         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16481_length_5086_cov_51.908368, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000138 DAOPVS010000000
VERSION     DAOPVS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16481_length_5086_cov_51.908368"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..3115)
                     /locus_tag="PKL11_09250"
     CDS             complement(422..3115)
                     /locus_tag="PKL11_09250"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNZ13828.1"
                     /translation="MTELPKAYDFAATEQRLYRWWEESGYFAPANDPRKPGFDPARAP
                     YVISIPPANVTGELHLGHALFVAMEDLMIRYHRMKGYSAMWLPGTDHAGIATQLQVEK
                     ALAKEGTTRDQLGRAAFVQRVWDWKNKYGGIITSQIRRLGASCDWQRERFTLDEGLSR
                     AVREAFVRLYEKGLIYRGLRMINWSPGLRTAVSDLEVEYSEEPGFLYYFKYRLADNPD
                     EFIPVATTRPETILGDTAVAVHPEDERYRAFVGRQVLVPILGRAIPVIADEYVDRAFG
                     TGALKITPAHDPNDYAIGQKFNLPMINILTLEAKINENGGRYTGMDRFECRKKLWADM
                     RAADLVIKEEPYTLNVPRSQRGGEIVEPMLSTQWFVKIQPLADAALAAVRDGRITIVP
                     ERFTRVYYNWLENIQDWCISRQLWWGHRIPVWYCADCGKMTVSRADPTACAHCGGTSI
                     QQDEDVLDTWFSSGLWPFSTLGWPEQTPDLAYFYPTQMMETGYDIIFFWVARMIMFGL
                     EFTGQVPFSTVYLHGIIRDEHGQKMSKTKGNSIDPLGVMDTMGTDALRFTLLVGSTPG
                     NDANLSLKKVEANRNFANKIWNAGRFIIGVLDSAPAEPQAAPDWTLADSWIWARLQTL
                     VRDVERLFSNHQYGEAGRQIYDFFWGEFADWYLEIAKLQMAAGGDRAHYTAYTLTRVL
                     DTCLRLLHPFTPYITEELWGHLKAAAGAKSARFAPAGGWEEALIIAHWPETKPVEGWE
                     EQKVADFALLQEAVRLIRNARTEKKLSPGQKIGASIAAGDKLPIFESQRAALCALAGI
                     DPDQLTLQAALAEKPQGQTALVVAGVEIFLSLAGLVDETAERARLEQELTEINSQIER
                     LAQLLNSPFAQKAPAAVVDKERQKLAAYQETAAKLKQQLGG"
     gene            complement(3487..4428)
                     /locus_tag="PKL11_09255"
     CDS             complement(3487..4428)
                     /locus_tag="PKL11_09255"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNZ13829.1"
                     /translation="MEKPTYSQLRQQMFAAWSAGDYAAALSTVDDFAAAFPEQSWQYP
                     FWRACLLARLGRPADALAELEAGLAAGYWWAPARLRSDEDLAALQDQPRFQAVLALCE
                     QKLQAASAQSRPECDLHLPPAGAEGAPLLVVLHGWGGNRKMYAPEWSAALKAGWGVAM
                     LQSSQVTSCATFGWDDVARAGDEIAAQVSDLLRAHNFDANRVVLAGFSQGGGEAIRLA
                     FEARFPVRGYLAVAPARLDMKELEDSPRGVAPRGVLLAGEADQRWADLAEQTADWLAA
                     HRLPHWFSLYPGLGHNLPPDFMDELPGWLAFIEGEAD"
BASE COUNT          951 a         1550 c         1590 g          995 t
ORIGIN      
        1 gaaatccagc aataatgggt taattataca gtaaaaggcc ggggtttggg aaatttggtt
       61 actgctcagg ctagatgaga ggctcatttg gctgaaagta tttcactcct ctgtggagtg
      121 cgggccgcac agcgcactgg aagtgcttca gcccgcatga ggtgcaggcc gcgcagcgca
      181 ctggaagtgc taaagcctgc actccagaag aaggccaaat ccgctcatag ctgagtagtt
      241 acgaaatttg tttatataat ttgtcggctg gttttggggc tgggggctgt cattgcaacg
      301 taggggcggt cctgtgtgac cgcccctaca agagccctgc tcgccacagg tggcgacata
      361 aatcagccgc tcccgaagga agcggctgaa atcctaaatc ttaaatccca aatcccaaat
      421 cctatccccc aagctgctgc ttcagcttgg cggcggtttc ctggtaggcg gccagcttct
      481 ggcgctcctt gtccaccacg gcggcggggg ccttctgggc aaacggactg ttgagcagct
      541 gcgccagccg ctcgatctgg ctgttgatct cggtcagctc ctgctccagg cgggcgcgct
      601 cggcggtctc atccaccagc ccggccagcg agaggaagat ctccaccccg gccactacca
      661 gcgccgtctg accctgcggc ttttcggcca gggccgcctg cagcgtcagt tgatccgggt
      721 caatgccggc cagggcgcac agcgcggcgc gctgtgattc gaagatcggc agcttgtcgc
      781 ctgcggcaat gctggcgcca atcttctgcc ccggcgagag cttcttctcg gtgcgggcat
      841 tgcggatcag gcgcacagct tcttgcagca gcgcaaagtc ggccaccttc tgctcttccc
      901 agccttccac cggtttggtt tcgggccagt gggcaatgat cagggcttct tcccaaccac
      961 cggcgggcgc aaagcgcgcc gatttggctc ccgcagctgc cttcaggtga ccccagagct
     1021 cctcggtgat gtaaggtgtg aaggggtgca gcagccgcag gcaggtatcc agcacgcggg
     1081 tcagggtata ggcggtatag tgcgcccggt cgccgcccgc ggccatttgc agcttggcaa
     1141 tttccagata ccaatcggca aactcgcccc agaagaagtc gtaaatctgc cgcccggcct
     1201 cgccgtattg atggttgctg aacaatcgct ccacatcccg caccagggtt tgcagccgcg
     1261 cccagatcca cgaatcggcc agcgtccagt cgggggcggc ctgcggctcg gccggagcag
     1321 aatcaagcac gccgatgatg aagcgcccgg cgttccagat tttgttggca aagttgcggt
     1381 tggcttccac cttcttcagg ctcaggttgg catcattgcc aggggtcgaa cccaccagca
     1441 gggtgaagcg cagcgcgtcg gtgcccatgg tatccatgac gcccagcggg tcaatcgaat
     1501 tgcccttggt cttgctcatc ttctgcccgt gctcatcacg gatgatgccg tgcaggtaca
     1561 ccgtgctgaa gggcacctgt ccggtgaact ccagcccgaa cataatcatg cgggccaccc
     1621 agaagaagat aatatcatag ccggtttcca tcatctgcgt ggggtagaag taggccaggt
     1681 cgggggtttg ctcgggccag cccagtgtgg agaacggcca cagcccggac gagaaccagg
     1741 tatccagcac atcttcatcc tgctggatgc tggtgccgcc gcagtgggcg caggcggtgg
     1801 ggtcggcgcg gctcacggtc atcttgccgc agtcggcgca gtaccacacg ggaatgcggt
     1861 gcccccacca caactggcgg ctgatgcacc aatcctggat gttttccagc cagttgtaat
     1921 agaccctggt gaagcgctcg ggcacgatgg tgatgcgccc gtcgcgcacg gcggccaggg
     1981 cggcatcggc cagcggctga atcttcacaa accactgggt cgagagcatc ggctccacaa
     2041 tttcgccgcc gcgctgcgag cgcggtacgt tcagtgtgta gggctcctct ttgatcacca
     2101 ggtcggcggc gcgcatgtcg gcccacagct tcttgcggca ctcaaaccga tccatgccgg
     2161 tgtagcgccc gccgttctcg ttgatcttcg cttccagcgt gaggatgttg atcatcggca
     2221 ggttgaactt ttggccgatg gcgtaatcgt tggggtcgtg ggcgggggtg atcttgagcg
     2281 caccggtgcc aaaggcccgg tccacatatt catcggcaat cacggggatg gcgcgcccca
     2341 gaatgggcac cagcacctgg cggcccacaa aggcgcggta gcgttcatct tcggggtgca
     2401 cggccaccgc cgtatcgccg aggatggttt cggggcgggt ggtggctacg gggatgaact
     2461 cgtcggggtt gtcggccagg cggtatttga agtagtacag gaagcccggc tcttcagaat
     2521 attccacttc caggtcgctg acggcagtgc gcagccccgg cgaccagttg atcatgcgca
     2581 ggccgcggta gatgaggccc ttttcgtaga ggcgcacaaa ggcctcgcgc acggcccggc
     2641 tgaggccctc atccagcgta aagcgctcgc gctgccagtc acacgaggcg cccagccggc
     2701 ggatctggct ggtgatgatg ccgccgtatt tgttcttcca gtcccacacg cgctgcacaa
     2761 aggcggcgcg gccgagctgg tcgcgggtgg tgccttcttt agccagggct ttttccacct
     2821 ggagctgggt ggcaatgccg gcatggtcgg tgccgggcag ccacatggcc gagtagccct
     2881 tcatgcggtg gtagcggatc atcaggtctt ccatggcgac aaagagggcg tggcccaggt
     2941 gcagctcgcc ggtgacgttg gccggcggga tggagatgac atagggcgcg cgggcggggt
     3001 caaaaccggg cttgcgcggg tcattggcag gggcaaaata gccgctttct tcccaccagc
     3061 ggtacaggcg ctgttcggtc gcggcgaaat cataggcttt tggtagttcg gtcatggtta
     3121 tcctcggtaa ttgaaattca aattaaatac cgtaacaaat cctaaaaaat ggggctcttt
     3181 ataaacaatt gtcatttctt gccaagcatc ataatccatc agtttagcaa aatggtcttt
     3241 ggagccatgt ttgcctcagc cttccgcctg aaatttccct ccctccgcga agcgcccttt
     3301 agggttaggg aggggctgcg actgttgctg ttacaggagc cgcccccagg ggtaggttag
     3361 acctaacccc ccgccctctc ctacggagtg ctgcgcaatc cctgaaggga agggggcttg
     3421 gcttctgtcc catttagatc ccttgttaca gtattatttt ataaaagttc accaacatct
     3481 gtccgttcaa tcggcttcgc cttcaataaa ggccagccag ccgggcagtt cgtccataaa
     3541 atcggggggc aggttgtggc ccaggccggg gtacaggctg aaccagtgcg gcaggcggtg
     3601 tgcggccagc cagtcggcgg tttgttcggc caggtccgcc cagcgctggt cagcttcacc
     3661 agccagcaag acaccgcgcg gggcgacgcc ccgcggagaa tcctccagtt ctttcatgtc
     3721 caggcgcgcc ggggccacag ccagatagcc gcgcaccggg aaacgcgctt caaaggccag
     3781 ccggatggct tcgccgccgc cctgcgaaaa acccgccagc accacgcggt tggcatcaaa
     3841 gttgtgggcg cgcagcaggt cgctcacctg cgcggcgatc tcatcaccgg cgcgggccac
     3901 atcgtcccag ccgaaggtgg cgcagctggt cacctgcgaa gattgcagca tggccacgcc
     3961 ccagcctgcc ttgagcgcgg cggaccattc gggggcatac atcttgcggt tgccgcccca
     4021 gccgtgcagc accaccagca gcggggcgcc ctcggcgccc gccggcggca gatgcaggtc
     4081 acattccggg cggctctggg cgctggcggc ctgcagtttt tgctcgcaaa gcgccaaaac
     4141 agcctgaaaa cgcggctgat cttgcagcgc ggccaggtct tcgtcgctgc ggagccgggc
     4201 cggcgcccac cagtacccgg cagccaggcc cgcttccagc tcggccaggg catcggcggg
     4261 gcgccccagc cgggccaaca ggcaggcccg ccagaagggg tactgccagc tctgctcggg
     4321 gaaggcggct gcaaagtcat ccacggtgct cagggcggcg gcataatcgc cagccgacca
     4381 ggcggcaaac atctgctgac gtaactgcga ataggttggc ttttccatgg gctttctccg
     4441 gttaaaaaac aaacaacccg catcatccat gaggacgaaa gggctgcttc cgtggtacca
     4501 cctcagttca ccggcggctg ccggcgcact catccccaga cgggatcatc tgggctgcgc
     4561 tgtaacgggc ttacccgtgc cgttctattc ttgcgggggc aatttcttcg gcaaacaggc
     4621 caggcgactt cggcatggat tgacccggag aggctctcaa ccgcggcctc gttctctgtc
     4681 gggatttcca gcagcctact cctcctggcg gagggcatat gcgcttgttg aatgtattat
     4741 acggtcgtta tgggggggtg tcaatgggtt gaatttggga ttgaggactt gtgattgaag
     4801 aagcgctgcc agggtgatgt gagggcctgc ccgccattcg ccgggtggcg ggcaggtcgc
     4861 gcagccctgg aaggcgcgcg cgtgtgtggc ccaaaggctc gggcggacac atagggccgc
     4921 ccctacacag cccaataaca gccggtgcgg cgccatgtct gtcgcacctg gaacctggca
     4981 cctccccctc gcacaaaccc gaccccaact tgaaacctga aacttgaaac ctgaaacgtt
     5041 aaacacagta taattaaccc atctcaccgg tgtggagcga cgatgt
//
LOCUS       DAOPVS010000139         5072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16548_length_5072_cov_60.094678, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000139 DAOPVS010000000
VERSION     DAOPVS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16548_length_5072_cov_60.094678"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKL11_09260"
     CDS             <1..480
                     /locus_tag="PKL11_09260"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ13830.1"
                     /translation="ETPVCQNPAKRLAGQLVDRWVVVLASDYLAPVARRWKGQISELA
                     KAWAQFEFLPEADHNSLAGILNPETALEKTFVLFLRAPSDHPRNRLRSNFTRQTMLIQ
                     ALGTDTVDALGDTPLAHIWTALQYGDYVSYYLAMAYGVDPTPVEALQALKNNLNAVK"
     gene            523..1314
                     /locus_tag="PKL11_09265"
     CDS             523..1314
                     /locus_tag="PKL11_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNZ13831.1"
                     /translation="MPYFWTLVRTSARQQMTYRMAMWAGLATNLFFGLLRAAVLLALY
                     NGQPVVNGLTMDGALTYVAFSQALIAFLTVFGSFDIMRTVYDGSISGDLLRPANFYFY
                     WMGREAGRAVVNLFWRGLVFIGLFALFYPIQTPASFTQWLLLTLSLLLSWLLNFTWIF
                     LTNLLAFWTPDAQGILRWLFGLAQLLSGWMVPLRLMPDWFSRICAFTPFPGMFNTSLE
                     VYLGLLPGPAVWQALGLQLAWLILFVILAQVIYRAGIRRLVIQGG"
     gene            1319..2125
                     /locus_tag="PKL11_09270"
     CDS             1319..2125
                     /locus_tag="PKL11_09270"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNZ13832.1"
                     /translation="MYTLSIYLRLLRAQLRSQFQYRASFWLDVFTTAVFSGVYFLSFA
                     LLLERFGQIDGWRLGEIAFLAGMTELTFALMDLIFSGFDPDFFAPNVQMGRLDQIFLR
                     PVSITVQLLGSRLVLRRLGRALEGLVILLFGIFASGMHWTLAKVLYLPILMVSQIFCM
                     GALFIAGSTITFWTVQRVEAVNIVTYGGVEMMSYPMSIYPAWIRRFFTYVLPFIFMNY
                     YPALFFLDKPDPLNMPAFAPFLAPFVAAILFWLALRFWRFGLNHYQSTGS"
     gene            2131..3168
                     /locus_tag="PKL11_09275"
     CDS             2131..3168
                     /locus_tag="PKL11_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ13833.1"
                     /translation="MTEPIIRVANLVKLFKVPIREKRGLAAVRSFFYRRFRTVRAVDD
                     VSFSLEPGELVGYLGPNGAGKSTTIKMLTGLLVPTSGQLTVAGFTPWKQRSAYVANLG
                     AVFGQRVTLWWDLPVIDSYELIRAMYRIPADRYNQNLKTFRDLLELDEFIQSPVRSLS
                     LGQRMRAELCAALLHDPRLVFLDEPTIGLDVVAKERIRQFIQHIHRQRGATIVLTTHD
                     LADVERLCSRVIILDVGRILYDGELSRLVQRFEGAHELRISAPEDSPALVLPGLPPPR
                     RDGRFLIYSFDPRQTPVAALLQQLNSHVAIADLEVHRPPLEDTIRRIYEQRLLLDDGS
                     HPDSPAGVEKR"
     gene            3206..3838
                     /locus_tag="PKL11_09280"
     CDS             3206..3838
                     /locus_tag="PKL11_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13834.1"
                     /translation="MKITETQKSIAQDGLLVVLAMLLLVLFTELLVHPIIKQFGTSGL
                     LVYTLGLLALGMYFLDRTISPSIPECQRAWHGMVGGLLTWSFIWLNDQTSQILISQSA
                     ALWMLTFSLVVAALWRRVLPIGVRFFFLVFTMNWGARMLLQAESILGQFWPVFQQIYD
                     FTGYIAIFFAVIGLLWIIFRARQRIMRMWIGVWVWFAILVALLAFWQPLL"
     gene            complement(3840..>5072)
                     /locus_tag="PKL11_09285"
     CDS             complement(3840..>5072)
                     /locus_tag="PKL11_09285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ13835.1"
                     /translation="MLSYHTCPLATLGGKDTGGMNVYVRELTRQLGQFGVHVDVFTRS
                     QDEHVPHVLHDLGYGNRVVHIPAGPEQPRPKPELAQYLDDFAAGIAQFAAEKNIRYDL
                     IHSHYWMSGIAGLKLKQTWGVPLVNMFHTLVLMKNRVARDDSEREGDYRLKGEQRIIK
                     EADRIIVATPAERSQLEFLYRANSRKLTVIPPGVDLGRFYPIPCDEARAVIGIPQAKC
                     MTLFVGRIEPLKGLETLLRAIAIMWNKGILTDCPHYLAIIGGEPDASAEHMNAEMARL
                     QALARELGISEIVLFLGKRGQDTLPYYYSAAQVLVMPSHYESFGMVALEAMACGTPVV
                     ASQVGGLAYLVQDGVTGYVVPDGNPEALADRLGALIQDPQLRRKMGQQASAAARDYAW
                     PVIGEQVIRLYQEMLVEK"
BASE COUNT          858 a         1731 c         1278 g         1205 t
ORIGIN      
        1 gagacgcctg tctgtcaaaa cccggccaag cgccttgccg gccagttagt ggatcgctgg
       61 gtggtcgtcc tggcttcgga ttatcttgcc cccgttgccc gccgctggaa aggccaaatc
      121 agcgagctgg ccaaggcctg ggcgcagttt gaatttttgc ccgaggccga ccataactcg
      181 ctggcgggca tcctgaaccc cgaaaccgcc ttagaaaaaa cctttgtgct gttcctgcgc
      241 gccccatccg atcacccgcg caaccgtctg cgctcaaatt ttacccgcca gaccatgctc
      301 atccaggcgc tgggcaccga tactgtggac gctctcggcg atacgcccct ggctcacatc
      361 tggacggccc tgcagtacgg cgattacgtc tcctactatc tggcaatggc ctacggcgtg
      421 gatcccactc ccgttgaggc actccaggct ctcaaaaata atctgaatgc ggtaaaataa
      481 gcgtttcggt tcttccccat ccggggagaa aggagcgccc ccatgccata tttttggacg
      541 ctggttcgca cgtctgcgcg ccagcagatg acctatcgca tggccatgtg ggccgggctg
      601 gcaaccaacc tgttctttgg gctgctgcgc gcggctgtgc tgcttgctct ctataatggt
      661 cagcccgtcg tcaacggcct caccatggac ggcgccctca cctacgtcgc tttttcccag
      721 gcgctgatcg ctttcctgac cgtctttggc tcctttgata tcatgcgcac cgtctacgac
      781 ggttccatct ctggcgacct gctgcgcccg gccaacttct acttttactg gatgggccgc
      841 gaagccggtc gcgccgtggt caatctgttc tggcgtggcc tggtttttat tggcctgttt
      901 gcgctttttt atcccattca gacgcccgcc agcttcacac agtggctcct gctgaccctc
      961 agcctgctgc tcagttggct gctgaacttc acctggattt tcctcacaaa cttgctggcc
     1021 ttctggacgc ccgatgccca gggcatcttg cgctggcttt ttggcctggc ccagctgctc
     1081 tctggctgga tggttcccct gcgcctgatg ccagactggt tcagccgcat ttgcgccttc
     1141 acgcccttcc ccggcatgtt caacacctcg ctggaggtct atctgggttt gctgccgggc
     1201 ccggctgtct ggcaggcgct gggcttgcag cttgcctggt tgatcctctt cgttattctg
     1261 gcccaggtga tctatcgcgc cggtattcgc cggctggtaa ttcagggagg ctgagagcat
     1321 gtacaccctc tccatctatc tgcgcctgct gcgcgcccag cttcgttccc agttccagta
     1381 ccgcgcctcc ttctggctgg atgtcttcac cacggcagta ttttccggtg tctatttcct
     1441 ttcctttgcg ctgctgctgg aacggtttgg gcaaattgac ggctggcggc tcggcgagat
     1501 tgcttttctg gctggcatga ccgagctgac cttcgccctg atggacctca tcttcagcgg
     1561 ttttgacccc gatttctttg cccccaatgt tcaaatgggc cgcctggatc aaatcttcct
     1621 ccgccccgtc agtatcaccg ttcaactact cggctcccgg ctggttttgc gccgcctggg
     1681 ccgcgccctc gaggggttgg taatcctgct ctttggcatc tttgcctccg gcatgcactg
     1741 gacgcttgcc aaggtcctct atcttcccat cctcatggtc agccagattt tttgcatggg
     1801 cgcgctgttt attgccggtt ccaccatcac cttttggacc gtccagcgcg ttgaagccgt
     1861 caacattgtc acctacggcg gggtggagat gatgagctac cccatgagca tctaccccgc
     1921 ctggatcagg cgcttcttca cttacgtcct gccctttatt tttatgaatt attaccccgc
     1981 cttgtttttc ctggataaac ccgatccatt gaatatgccc gcttttgcgc cgtttctggc
     2041 gccctttgtt gccgccattc tgttctggct ggcgctccgt ttctggcggt ttggcctcaa
     2101 tcactatcaa agcaccggga gttaggtatc atgaccgagc ccattattcg tgtagccaat
     2161 ttggtcaagc tgttcaaagt ccccatccgt gaaaaacgcg gcctggctgc cgtccgcagc
     2221 tttttctacc gccgttttcg caccgtccgc gccgtggatg atgtctcctt ttccctcgag
     2281 cctggtgagc tggtcggcta cctgggcccc aacggggcgg gcaagtccac caccatcaaa
     2341 atgctcactg gcttgctggt gcccaccagc ggccaactga ccgtggcggg cttcacgccc
     2401 tggaaacagc gctccgccta tgtcgccaac ctcggagcgg ttttcggtca gcgcgtcacg
     2461 ctctggtggg atttgcccgt gatcgattct tacgagttga tccgcgccat gtaccgcatt
     2521 cccgccgacc gctacaatca aaacctcaaa acctttcgag atctgctgga actggatgaa
     2581 ttcatccagt caccggttcg ctcgctgtcg ctgggccagc gcatgcgcgc cgagctttgc
     2641 gccgccctgc ttcacgatcc ccgcctggtc ttcctcgatg aacccaccat cggccttgat
     2701 gtggtggcta aagagcgcat ccgccagttc atccagcaca ttcaccgcca gcgcggcgcc
     2761 accatcgtcc tcaccaccca cgacctcgcc gatgtcgagc ggctttgcag ccgcgtgatc
     2821 atcctggacg tgggccgcat cctctacgat ggcgaattga gccgcctggt ccagcgtttt
     2881 gaaggcgccc atgagctgcg catttcagct cccgaagaca gccccgcgct ggtgctgccc
     2941 ggcttaccgc cgccccgccg tgatggccgc ttcttgatct actcgtttga cccccgccag
     3001 acccccgtgg ccgcgctctt gcagcagctc aacagccatg tcgccatcgc cgacctcgaa
     3061 gttcaccgcc ctccgctcga agacaccatc cgccgcatct acgaacagcg cctgctcctg
     3121 gatgacggat ctcacccaga ttcccccgct ggcgttgaaa agcggtaaaa ttaagctgca
     3181 tcacttctgc atctgaggac tgttcatgaa aattactgag acgcaaaaaa gcattgccca
     3241 ggacggcctc ctggtcgtct tagccatgct gctgctggta ttgttcaccg agctgctggt
     3301 tcaccccatc ataaagcagt tcggcacctc cggcttgctg gtttacaccc tgggcctgct
     3361 ggccctcggc atgtattttc tggaccgcac catctcccct tcaattcctg aatgtcagcg
     3421 cgcctggcat ggcatggtcg gcggcctgct gacctggtct ttcatctggc tcaacgatca
     3481 aacttcccaa atcctcatca gccaatccgc cgccctctgg atgttgacct tctccctggt
     3541 cgtggcggcc ctctggcggc gtgtgctccc cattggcgtg cgcttctttt tcctcgtctt
     3601 caccatgaac tggggcgccc ggatgctcct ccaggctgaa agcatcctgg ggcagttctg
     3661 gccggttttt cagcagattt acgatttcac cggctatatt gccatcttct ttgccgtcat
     3721 tggcctgctc tggatcatct tccgcgcccg ccagcgcatc atgcgcatgt ggattggggt
     3781 ctgggtctgg ttcgccatcc tggttgcact gctggccttc tggcagcccc tgctctaact
     3841 cacttctcca ccagcatctc ttgatacagc cggatcacct gctccccaat cacgggccag
     3901 gcataatccc gcgccgcggc gctggcctgc tgccccattt ttcgccgcag ctgcgggtct
     3961 tgaatcaatg cccccagccg gtcagccagt gcctccgggt tgccatcggg caccacatac
     4021 cccgtcaccc catcctgcac caggtaagcc agcccgccca cctgtgaggc caccaccggc
     4081 gtcccgcagg ccatcgcctc cagcgccacc atgccaaatg attcgtagtg cgaaggcatc
     4141 accagcacct gcgccgcaga ataataatag ggcagcgtat cttgtccccg tttgcccaaa
     4201 aacaacacaa tctcactgat ccccagctcg cgcgccagcg cctgcagccg ggccatctct
     4261 gcattcatat gctccgccga ggcatccggc tccccaccga tgatcgccag gtaatgcggg
     4321 cagtcggtca aaatgccctt attccacatg atcgcaatcg cccgcagcaa ggtttccagc
     4381 cctttcagtg gctcaatccg gcctacaaac agcgtcatgc actttgcctg cggaatgcca
     4441 atcactgccc gggcctcatc gcacggaatc gggtagaacc gccccaggtc cacccccggc
     4501 ggaatcaccg tcaacttgcg ggaattggcc cgatacaaaa actcaagctg cgagcgttct
     4561 gccggggtcg ccacaataat ccgatccgcc tctttgataa tgcgctgctc gcccttcaga
     4621 cggtaatcgc cctcgcgctc gctgtcatcc cgcgccaccc ggtttttcat caacaccagc
     4681 gtatggaaca tattgaccag cggcacgccc caggtttgct tcagcttcaa ccccgcaatc
     4741 ccgctcatcc agtaatggct gtgaatcaaa tcatagcgaa tatttttctc tgccgcaaac
     4801 tgggcaatcc cggcggcaaa atcatccagg tactgcgcca gctctggctt gggccgcggc
     4861 tgctcggggc cggccggaat atgcaccacc cggttcccgt accccagatc atgcagcaca
     4921 tgcggcacat gctcatcctg cgagcgggta aaaacatcca catgcacccc aaactgcccc
     4981 aactgccggg tcagctcgcg cacatacaca ttcatcccgc cggtatcttt gccccccagc
     5041 gttgccagtg ggcaggtgtg ataagagagc at
//
LOCUS       DAOPVS010000140         5037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16702_length_5037_cov_59.660377, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000140 DAOPVS010000000
VERSION     DAOPVS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16702_length_5037_cov_59.660377"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1925
                     /locus_tag="PKL11_09290"
     CDS             372..1925
                     /locus_tag="PKL11_09290"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNZ13836.1"
                     /translation="MPVEDPRILALREMRAKARLGGGEERIAAQHAKGKLTARERLEQ
                     LLDPGTFQELEPFVVQGHDETNPDFDKILGDGVVTGFGRIDGRLVYAYSQDFTVQGGS
                     LGEMQSHRICAAMDAAVRAGAPVIGLIDSGGARIQEGVRSLGGYAEIFRRNALYSGII
                     PQISVIMGPCAGGASYSPALTDIIIMVEKSSYMFLTGPDVIKTVTGEVVDQETLGGAA
                     THHSVSGLAHLTAADEPGAFAEVRRVLSYLPQNHVENPPFVQTGDDANRMDEALNQLV
                     PIDPSAPYSMHDAIEYIVDRGSFTELLGGFARNAIVGLARMGGMSVGIVSQEPDTLGG
                     SIDIDASDKITRFVRFCDCYNIPIVTFVDSPGFIPGTDQEYRGIIRHGAKVLYAYAEA
                     SVPKISIVTRKAYGGAYVVMSSKYMGTDICYAWPSAEIAVMGADGAANIVYRRQIKEA
                     ADPAAERARLVEEYRQRFCNPYAAANAGYIDEVIEPRETRSRVIAALIALRDKTVQAL
                     PRKHGNMPV"
     gene            1946..2305
                     /locus_tag="PKL11_09295"
     CDS             1946..2305
                     /locus_tag="PKL11_09295"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNZ13837.1"
                     /translation="MSDIVSALMMTLIGMGLVFVVIVIMWGFMWALVKFTAEKPNGAA
                     EANNDPEASAPVEAVLPTGLKARAAAAAVAVALALGSKSASLAAPTSNSVSAWQAAQR
                     ANYQGLRDGLYLRKPKG"
     gene            2321..2749
                     /locus_tag="PKL11_09300"
     CDS             2321..2749
                     /locus_tag="PKL11_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNZ13838.1"
                     /translation="MKIKVTIADQTFEVEVGDLQARPILATIDGETFEVSPETAAAAP
                     AAAPAASARPAPVAAPAASRAPAAGGGGKQVVAPIPGVIISITVKPGDEVKVGQELLI
                     LEAMKMKNAIRANRAGTVATILVAAGDQVRHSQPLIEFTD"
     gene            2757..3929
                     /locus_tag="PKL11_09305"
     CDS             2757..3929
                     /locus_tag="PKL11_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ13839.1"
                     /translation="MMDLTQVFQYLLKGFEAFTWANGVMILLGCVLVYLAVAKEIEPV
                     LLLPIGVGCIFANLGMPAGANIPAELEHNWMNVIYNAGIKTELFPLLIFVGVGAMIDF
                     RPLLAQPRMVLLGAAGQFGIYGTLILAVLLGFPMNEAASIGVIGAIDGPTAIFVTSQI
                     APKLLGPVAVAAYSYMSLVPIIQPPIMKLMTTRKERLIRMEYAPIPVSRLAVVLFPII
                     VTVVVSLIAPDAAPLIASLMLGNLIREVGVVDRLNKAVQNEIINVSTLFLGLAIGATM
                     TASSFLFDPTTGIQWKTLMILGLGLLAFVIDTVAGLLFGKLMCVLSGFKINPLIGACG
                     ISAFPMSGRIAAKVAQDEDFSNFILMHAMGSNTAGQLGSVIAGGLLLALVRGLGFG"
     gene            complement(4037..>5037)
                     /locus_tag="PKL11_09310"
     CDS             complement(4037..>5037)
                     /locus_tag="PKL11_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13840.1"
                     /translation="YNPSLKSYVQYFTNGAIYYDQNSTPENARLLPLGCYAMEDVLGH
                     PVCNLRDRTHDEISEPFIAALWAATAQLDIGKPLTEPYTAPDGLWEQAYEGAVIFAAA
                     DNPNAVGLRPAARSTNFQTSELYDPNTPYDTNTAVFITVDEVTGQGQFVPIIFHTYIM
                     SHGGYAWSGKPISIYIPQQGGGYRQCFENYCLDIDLTTGQVSLARSLGDHYIQNVSYV
                     PAAALSVPLTADRVALSFYDSEVSAGQARIVIWALDAKTAAPLAGLSGQIDITQKDGS
                     IVSYPLPLTDAAGAASLTLAIPAGYMNGEIVNYTVCLSLPNTDPKCASDGYLVWGQ"
BASE COUNT          950 a         1572 c         1517 g          998 t
ORIGIN      
        1 cccgcgccgc tccaggtgaa tcccacccag gcgcagggct gtctggcacc ataattctag
       61 cataagacgc cccgcccgac catccagaaa caggccgcaa acggcctgcc cggctgccat
      121 ccggcgacca gatgggcccc gtatggggca caagtcgccg ccggtggtga gcggggctct
      181 tgcactggcg caatgagagg cacccgcgcc ataacctaaa attctaggat aaatatccac
      241 ttaaaagggt gcgttattca taccgtgccc ggggcaagat ggcgtaaaat tggcgtggca
      301 gtcggctttt tggggccggt ctatcttcat gccccggagc cgcaccaaat tttcgatcag
      361 aggaatttcc tatgcccgta gaagatcccc gcatccttgc attgcgcgaa atgcgcgcca
      421 aggcccgcct gggcggcggc gaagaacgca ttgccgctca gcacgccaaa ggcaagctga
      481 ccgcgcgcga acgtctggaa caactgctcg acccgggcac gttccaggaa ctggagccgt
      541 ttgtcgttca gggacatgac gaaaccaacc ccgatttcga caaaattctg ggcgatggcg
      601 tggtcaccgg ctttggccgc attgatggcc gcctggtgta cgcctactcg caggatttca
      661 ccgtgcaggg cggttcgctc ggcgagatgc agagccacag aatctgcgcc gccatggatg
      721 ccgccgtgcg cgctggcgcc ccggtgatcg gcctgattga ctcgggcggc gcccgtattc
      781 aagaaggcgt ccgcagcctg ggtggttatg ctgaaatctt ccgccgcaac gccctctact
      841 ccggcatcat cccgcagatc agcgtcatca tggggccgtg cgcggggggt gcgtcttact
      901 cgcccgccct caccgatatc atcatcatgg tcgagaaatc gtcctacatg ttcctcaccg
      961 gccccgatgt catcaagacc gtgaccggcg aagtggtgga ccaggaaacc ctgggcggcg
     1021 cggccacgca tcacagcgtc agcggtctgg ctcacctgac cgccgccgac gagcccgggg
     1081 cctttgccga ggtgcgccgg gtgctcagct acctgcccca gaaccatgtg gaaaacccgc
     1141 cctttgtgca gaccggcgac gacgccaacc gcatggacga ggccctcaac cagttggtgc
     1201 cgattgaccc ctcggccccc tactcgatgc acgacgccat tgaatatatt gtggaccgcg
     1261 gctccttcac tgaactgctg ggcggctttg cccgcaacgc cattgtgggc ctggcccgca
     1321 tgggcggcat gtcggtgggc attgtctcgc aggaacccga taccctcggc ggctcgattg
     1381 acattgacgc ctccgacaag atcacccgct ttgtgcgctt ctgcgactgc tacaatattc
     1441 cgatcgtcac cttcgtggac tcccccggct tcatccccgg caccgaccag gaataccgcg
     1501 gcatcatccg ccacggcgcc aaggtgctgt atgcctacgc cgaagccagc gtgcccaaga
     1561 tcagcatcgt cacccgcaaa gcctacggcg gcgcgtatgt ggtgatgagc agcaagtaca
     1621 tgggcaccga tatctgctac gcctggccca gcgccgaaat tgcggtgatg ggcgccgatg
     1681 gcgcggccaa tattgtgtac cgccggcaga ttaaagaagc cgccgacccc gccgccgagc
     1741 gcgcccgcct ggtggaagaa tatcgccagc gcttctgcaa cccctatgcc gccgccaacg
     1801 ccggctacat tgacgaagtg atcgaaccgc gcgaaacccg cagccgcgtg attgccgccc
     1861 tcattgccct gcgcgataaa accgtgcagg ccctgccgcg caagcacggc aacatgcccg
     1921 tctaaccgcg gagaggagca tctccatgag tgatattgtc tctgcattga tgatgaccct
     1981 gatcggcatg gggctggtct ttgttgtcat cgtgattatg tggggtttca tgtgggcgct
     2041 ggtcaagttc acggccgaaa agcccaacgg tgcggctgaa gccaacaacg accccgaagc
     2101 ctctgcccct gttgaagccg tgctgcccac cgggctcaaa gcccgcgctg cggcggctgc
     2161 ggtggcggta gccctggccc tgggcagcaa gtcggccagc ctggccgccc ccaccagtaa
     2221 ctcggtcagt gcctggcagg cggcgcagcg cgccaactac cagggcctgc gagacggcct
     2281 gtatcttcgc aaaccgaagg gttaattcaa aggtgtgatg atgaaaatta aagtaacgat
     2341 tgcagatcaa acatttgagg tcgaggttgg cgatttgcag gcccgcccga ttctggccac
     2401 gattgacggc gaaacctttg aggtctcgcc cgagaccgct gcggcagctc cggctgcggc
     2461 cccggcggcc tcggcccgcc cggcccctgt ggcggccccg gctgccagcc gcgccccggc
     2521 tgcgggcggc ggcggcaagc aggtggtggc gcccatcccc ggtgtgatca tctcgatcac
     2581 ggtcaaaccc ggtgatgagg tcaaagtggg ccaggaactg ctgatcctcg aagcgatgaa
     2641 gatgaagaac gccatccgcg ccaaccgcgc cggcaccgtt gccactatcc tggtcgccgc
     2701 gggtgatcag gtccgccaca gccaacccct gattgaattc accgattaag gggtgaatga
     2761 tggacctgac acaggttttt caatacttac tgaaagggtt tgaagccttc acctgggcca
     2821 acggcgtgat gattctgttg ggctgcgtgc tggtgtattt ggccgtggcc aaagagatcg
     2881 aacccgtgct gctgctgccg attggtgtgg gctgtatctt tgccaacctg ggcatgccgg
     2941 ccggcgccaa catccctgcc gaattagagc acaactggat gaacgtgatc tacaatgcgg
     3001 gcatcaagac cgaactcttc ccgctgttga tctttgtggg cgtgggcgcc atgattgact
     3061 tccgcccgct gctggcgcag cccaggatgg tgctgctggg tgcggcgggt cagtttggca
     3121 tctatggcac gctgattctg gctgtgctgc tgggcttccc gatgaacgaa gccgcctcca
     3181 ttggcgtcat tggcgccatt gacggcccca cggccatctt tgtcacctcg cagattgcac
     3241 ccaagctgct gggcccggtg gcggtggctg cctactctta catgagcctg gtgcccatca
     3301 tccagccgcc gatcatgaag ctgatgacca cccgcaaaga gcgcttgatc cgcatggaat
     3361 atgcgcccat ccccgtctcc cggctggccg tggtgctgtt ccccatcatc gtcacggtgg
     3421 tggtcagcct catcgccccc gacgccgctc cgctgatcgc ctcgctgatg ctgggcaacc
     3481 tgatcagaga ggtgggcgtg gtagaccggc tgaacaaagc cgtgcagaac gagatcatca
     3541 acgtctccac cctcttcctg ggcctggcca ttggcgccac catgacggct tccagcttcc
     3601 tcttcgatcc gacgactggc atccagtgga aaaccctgat gatcctgggg ctgggcctgc
     3661 tggcctttgt gatcgacacg gtagctggcc tgctctttgg caagctgatg tgcgtgctct
     3721 cgggcttcaa aatcaacccg ctgattggcg cctgcggcat cagcgcgttc cccatgtcgg
     3781 gccgcattgc ggccaaagtg gcgcaggacg aagatttcag caacttcatc ctgatgcacg
     3841 ccatgggctc caacaccgcc gggcagctcg gcagcgtcat tgctggcggc ctgctgctgg
     3901 cgctggtgcg cggcctgggc ttcggttaaa tcaacacttc tcgggcaaat taaatccccc
     3961 cggtgctttt gcgccggggg gtgtttgttt tttgggggtt aagttttact tttcaggtgc
     4021 aaggggcggt tttcacctac tggccccaga ccaggtagcc atccgaggcg catttgggat
     4081 cggtattggg caggctgagg cagacggtat aattcacgat ctcgccgttc atataacccg
     4141 ccggaatcgc cagggtcagg ctggcagcgc cagccgcgtc ggtgagcggc agcggatagc
     4201 tgacgatgga gccgtctttt tgggtgatgt cgatctgccc ggaaaggcct gccagtgggg
     4261 cggctgtttt cgcatccagc gcccagatca caattctggc ctgacccgcg gacacctcgc
     4321 tgtcataaaa gctgagagcc actctgtcgg cggtcaacgg gactgacagt gccgccgctg
     4381 gcacatagct gacgttttgg atatagtggt cacccaggct gcgcgccagc gagacctgcc
     4441 ccgtggtcag gtcaatatcc aggcagtaat tttcaaagca ctggcggtag ccgccgccct
     4501 gctgaggaat gtaaattgag atgggtttgc ccgaccacgc atacccgccg tgagacatga
     4561 tataggtatg gaagatgatc ggcacaaact gtccctgtcc ggtgacctca tccacggtga
     4621 tgaaaaccgc cgtattggta tcatagggcg tgttggggtc atagagctca gaggtttgaa
     4681 agttggttga ccgggccgcc gggcgcagcc ccacagcatt gggattgtca gccgcggcaa
     4741 agatcactgc gccctcatac gcctgctccc acagcccatc cggggcggtg tagggctcag
     4801 tgagcggctt gccaatatcc agttgggcgg ttgccgccca gagcgcagct ataaaaggct
     4861 cggaaatctc gtcatgggtg cggtctcgca gattgcagac tgggtgcccc aaaacatctt
     4921 ccatcgcata gcaccccagc ggcagcagcc gggcgttttc gggggtgctg ttttgatcat
     4981 aataaatggc cccgttggta aaatactgca catagctttt tagcgacggg ttgtatt
//
LOCUS       DAOPVS010000141         5021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16766_length_5021_cov_59.057189, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000141 DAOPVS010000000
VERSION     DAOPVS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16766_length_5021_cov_59.057189"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PKL11_09315"
     CDS             complement(<1..875)
                     /locus_tag="PKL11_09315"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNZ13841.1"
                     /translation="MKTKLSIGIRLLVVFALALGGAFILIRPVGATTIVDGGVIAAGE
                     TIDDDALVTGEKVIVDGTVNGILFAFAETITINGTINGDVIAMGSTVIVNDGAEINGN
                     LFIGCQSAQVNGHITGSVLGGMTSMIIGDTALVDRNLYFGGYSLEIESGSVITRDASV
                     GAYQLILAGEIQRDLNLNAGALDMTGTIGGDATLSISEPQPSGTIPAFGMPNDMPQPI
                     APGLRIAPEAVIAGHLTYTSPEEQASAIQSQPGGGVTYNKSVVDPSSPTPQRSPRPPM
                     LQWLWSFLKNLFQLLV"
     gene            complement(961..1365)
                     /locus_tag="PKL11_09320"
     CDS             complement(961..1365)
                     /locus_tag="PKL11_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNZ13842.1"
                     /translation="MPTLTNTELISQALTYLYPRTLDHDNSAGGVACALLSASGKLYF
                     GVCMDISSGIGFCAEHSAIAAMITAGETRIAKIVAVAGGGVVLPPCGRCREFMYQIDG
                     ANLAETDVIMSPTTTLKLKDLLPHPYDEVWSV"
     gene            complement(1442..2779)
                     /gene="glnA"
                     /locus_tag="PKL11_09325"
     CDS             complement(1442..2779)
                     /gene="glnA"
                     /locus_tag="PKL11_09325"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNZ13843.1"
                     /translation="MNAKDILAHVAADHVKFISLQFTDVTGVVKSLDIPVERLEGCIE
                     NGIWFDGSSVEGFARIQESDMHLRMDLDTYAVLPWTPLEMRRARFFCDIYQPDGSPFP
                     GDPRGALKRTLSALRDRGWIYNVGPEPEFFLFKRNGGEKIHPVPHDVGGYFDFSPNDE
                     AVRVRTELMDALRSMGLDVEVGHHEVALGQHEIDFRFDDALRTADNVLTLKYTVKAIA
                     AQNGLVASFMPKPIFGINGSGMHVHQSLSDFSGKNLFSAPNHGMCLSPLAIHFIAGQL
                     AHARALAGVVAPSVNSYKRLVPGYEAPVYVGWAQTNRSALIRIPRYMPGQEQAARAEL
                     RCPDPSCNPYLAFTAMLAAGLDGIDRQLTPPEPLNNVNVYELEPDIRLQMGIEELPGS
                     LLEALNELARDEVLQQSLGSEVYAAFQRAKLAEWDEYRTHVTDWEIEHYLELT"
     gene            3249..3890
                     /locus_tag="PKL11_09330"
     CDS             3249..3890
                     /locus_tag="PKL11_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNZ13844.1"
                     /translation="MKQFTESSSPLTVVFDLGGVLMDWNPRYLYRKLFASEKAMEDFL
                     VEIDFFEWNQQQDAGRPYTVAVAELCKIYPQHSDLIRAYDERYLESLKGPIWETVTIL
                     RRLWQAGVPLFGLSNWPAEKFRLVRPVYEFFDWFQEIVISGEVGAAKPDPKIFEVLLD
                     KVGRPARDCLLIDDSSGNIEAAKRLGFQTIHFEHPAQLEAELIQQELLPILQH"
     gene            3896..4756
                     /locus_tag="PKL11_09335"
     CDS             3896..4756
                     /locus_tag="PKL11_09335"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ13845.1"
                     /translation="MTMRNSIALKNIFWIWLLLGMAACTPAVAVEPQPTATPTTALSR
                     LPTHTPTPQPSPTPTDTLTPTPAAPEVCSPLEGVALADLAEIVVNPYAPPPMGSDQPH
                     QGIDLAQLDPATRIALKGLPVHAVMAGDVVMVLNDRFPYGNAVLIKVPLESVPAGWLE
                     GLALPEPIPTLSAPPALTCPQPETLPDWDNSRRAYYILYAHLQEPPTVQVGEVINCGQ
                     GLGTIGDSGNALNPHLHVEIRVGPADAVLGNMAHYDNSATPAEMTAYCQWRVSGWFQP
                     IDPLRLLENK"
     gene            4821..>5021
                     /locus_tag="PKL11_09340"
     CDS             4821..>5021
                     /locus_tag="PKL11_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13846.1"
                     /translation="MKQNIKLWIVLASAGLLLGLALIFWPDGQPRLAEGARPVEIWLA
                     AEDTPQTLMSAAGTPIELLTEAG"
BASE COUNT         1183 a         1269 c         1487 g         1082 t
ORIGIN      
        1 accaggagtt ggaagaggtt tttgaggaag ctccacagcc attggagcat gggcggacga
       61 ggcgagcgct gcggggtggg cgatgaggga tctaccaccg atttgttgta ggttacaccg
      121 ccaccgggct gcgattgaat ggcagaggct tgctcttccg ggctggtgta ggtcaaatga
      181 ccggcaatga ccgcttcggg ggcaatgcgc aagccgggtg cgatgggctg gggcatatca
      241 ttaggcatgc caaaggcggg gatggtgccg gagggctgtg gttcggagat acttagcgtg
      301 gcatcgccgc caatggtgcc ggtcatatcc agggcaccag cattaagatt gaggtcacgc
      361 tggatttcac cggcaaggat cagttgatag gcgccaaccg aagcgtcgcg ggtgataacc
      421 gagcctgatt caatttcgag gctgtaaccg ccaaagtaga ggttgcggtc caccagagcg
      481 gtatcgccaa tgatcattga cgtcataccg cccaaaacgc tgcctgtgat gtgaccattc
      541 acctgagccg attggcaacc tatgaacagg ttgccgttaa tctcagcacc gtcattgaca
      601 atcactgtgc tgcccatggc aatcacatca ccgttgatgg tgccgttaat ggttatcgtt
      661 tcagcaaagg cgaacaggat gccgttgact gtaccatcca cgatgacttt ttcacccgtg
      721 acaagggcgt cgtcatcaat ggtttcgcca gccgcgatta cgccgccatc cacaatggtg
      781 gtggcgccaa ccggacggat gaggatgaag gcgcccccca gcgccagagc gaatacgaca
      841 agcagccgta ttccaatgga taatttggtt ttcacaagtt cctccaggca agaaaaaatt
      901 acgcaaaatt acagagcatt cgtaaaaata tacgaatatt acgctcaaaa agttgcgaaa
      961 ttagacgctc cagacttcat cgtagggatg gggaagaaga tctttgagtt tcagggtggt
     1021 ggtggggctc ataatgacat cagtttcggc caaattagcg ccgtcaatct ggtacataaa
     1081 ttcacggcag cgaccacagg gcgggagaac gaccccacct ccagcaacag caacaatttt
     1141 tgcaatgcgc gtctcgccgg cggtaatcat ggcggcaatg gcgctgtgct cggcgcagaa
     1201 gccaatgccg ctgctgatat ccatgcagac gccaaagtag agcttgccac tggcgctaag
     1261 caaggcacag gctacaccgc cagccgagtt gtcgtgatcg agggtgcggg gatagaggta
     1321 cgtgagggcc tgggagatca gttcggtatt cgtcagggtg ggcatggtgg atttcctttc
     1381 ggcaggtatt ataaccgttg cagggcggct tttggagccg ccctggagca acgcgcgggc
     1441 attaagtcaa ttcgagataa tgttcaattt cccaatcggt gacgtgggtg cggtattcat
     1501 cccattcagc cagtttggcg cgttggaatg ccgcatagac ctcggagccc agggattgtt
     1561 gcaacacctc atcgcgggcc agttcattca gcgcctcaag cagagagccg gggagttcct
     1621 ctattcccat ttgcaggcgg atatcgggtt ccagttcata gacattgaca ttattgagcg
     1681 gttccggcgg ggtgagctgg cggtcaatgc catccaaacc ggcggcaagc atggcggtga
     1741 atgccagata ggggttacag gaaggatcgg gacagcgcag ttcagcgcga gcggcctgtt
     1801 cctgtccggg catgtagcgg gggatgcgga ttaacgctga acgattggtc tgcgcccagc
     1861 caacataaac cggggcttca taaccgggga ccagccgttt ataggaattg acggaaggcg
     1921 ccaccacacc tgccagagcg cgggcatggg caagttgacc ggcaataaaa tggattgcca
     1981 gcggtgaaag gcacatgccg tgattgggtg ccgagaagag atttttgcca gaaaaatcgg
     2041 acaatgattg gtgaacgtgc atgcccgagc cgttgatgcc aaagattggt ttgggcatga
     2101 aggaagccac cagaccattt tgagccgcaa tggctttaac ggtgtacttg agagtcaaaa
     2161 cattgtctgc cgtgcgcaaa gcgtcatcaa agcggaagtc aatttcatgc tgaccaaggg
     2221 caacttcatg atgtccaact tcgacatcca gccccattga gcggagcgca tccatcaatt
     2281 cggtgcgtac ccggacggct tcatcgttgg gggagaaatc aaagtagccg ccaacgtcat
     2341 gtggaacggg atggattttc tcgccgccat tgcgtttgaa gaggaagaac tctggttcgg
     2401 ggccaacatt gtaaatccag ccgcggtcac gcagggcaga cagagtgcgc ttgagcgccc
     2461 cgcggggatc gccggggaag ggtgagccat ccggctggta gatatcacag aaaaagcgcg
     2521 cccgacgcat ttcgagcgga gtccagggta agacggcata ggtgtccagg tccatgcgca
     2581 ggtgcatgtc tgactcctgg atacgggcaa agccttcgac agaagagcca tcgaaccaga
     2641 tgccattttc aatacagcct tccaggcgct caacggggat gtcgaggctt ttgaccacgc
     2701 cagttacatc ggtaaattgc agcgagataa acttgacatg atctgcggct acatgagcca
     2761 aaatatcttt tgcattcatc agaacctcca ggcataaaaa agtatacgga tatgcccggg
     2821 catatccgtt tttgaacagg taatattcat aacaggggat tgcggcaggt cttccaggtc
     2881 aaatcgccag tcaggcgttc agactgctga ctggatcact acgccaaagc tgtgccgcac
     2941 gttgccgcgc ggttttgtat ggcgcttttg ggaaggagaa ggaagcccgt gatcaggctc
     3001 cagaggcatc cgctgatcca tcgattttct cccgcttgcc cccgcgggat gagcgctccc
     3061 ttcgcaggga ggaacctcca cctcgtcgtc tcggcactgg gccgagattc agccgctata
     3121 tctccagtat tgggttgtca atgcggttga tggtagcaca ggaagggaaa ggctgtcaag
     3181 ggtgtctgga catcttgatc agcagcaaaa catttttcaa ggcagcagca ggtggtacaa
     3241 tcactgccat gaaacaattc actgaatctt catcaccatt gacggttgta ttcgatctgg
     3301 ggggcgtttt aatggactgg aatccacgat acctgtatcg gaagctcttt gccagtgaaa
     3361 aggccatgga agattttctg gtcgaaattg acttttttga gtggaatcaa cagcaagatg
     3421 ctgggcgccc ttatacggtg gcagtggctg aactttgcaa gatttatcca caacacagcg
     3481 atttgattcg cgcctatgat gagcgttacc tggaatcgct caaagggccg atttgggaga
     3541 ctgtgactat cctgcggcgg ttatggcaag ccggggtgcc cctgtttggg ttgagtaatt
     3601 ggcctgcaga gaagtttcgg ttggtgcgcc cggtatatga attttttgac tggtttcagg
     3661 aaattgtcat ttcgggcgag gttggggcag ccaaacccga cccgaagatt tttgaggttt
     3721 tactggacaa ggtggggcgc ccggcgagag attgcctgct gattgatgat tccagcggca
     3781 atattgaagc tgcaaaaaga ttgggattcc agacgatcca ttttgaacat ccagcccaac
     3841 tggaagccga gttgatccag caagagctgc tgccgattct gcagcattag cgtcaatgac
     3901 tatgcgcaat tcaatcgccc tcaagaacat cttctggata tggttgcttc tgggtatggc
     3961 ggcctgcacg ccagccgttg cggttgagcc gcagccaacc gcaacaccca caacagccct
     4021 gtccagattg ccaactcata cccccacccc acaacccagc ccaacaccga ctgatacgct
     4081 cacgccgaca cccgccgctc cagaagtttg ctcacccctg gagggcgtgg cactggcaga
     4141 cctggcagag attgtggtta atccgtatgc accgccgccc atggggtcag atcaacccca
     4201 tcagggaatt gacctggcgc agttagaccc ggcaacccgg attgcgctaa aaggcctgcc
     4261 ggttcatgcg gtgatggcgg gggacgttgt gatggtcttg aatgaccgct ttccatatgg
     4321 gaatgctgtg ctgatcaaag tgccattaga gtcagtgccg gcggggtggc tggaaggtct
     4381 ggcgctgcca gagcccatac caacgctttc ggcgccgcca gccttgacct gcccgcagcc
     4441 tgaaaccctg cctgattggg ataactcccg gcgggcatac tatattttat atgcacatct
     4501 acaagagccc cccacagttc aggtcggtga ggtgattaac tgcggtcaag gtctggggac
     4561 gattggcgac agtggtaatg cgctcaaccc gcatttgcat gtggagatcc gggtggggcc
     4621 ggcagatgcg gtgctgggga atatggccca ttacgataac tcggccactc cggcagaaat
     4681 gacagcctac tgccagtggc gcgtgagcgg gtggtttcag cccatcgatc cgttgaggct
     4741 gcttgagaat aaatgagacg gcagcaaaat cgattaccgg gtatcagaca ggccaaaaat
     4801 ccggtacaat tgaaagcgcg atgaaacaaa acatcaagtt atggattgtt ctggcgtctg
     4861 ccggactgct actggggctg gcactgattt tctggcccga tggacagccg cggttggcgg
     4921 aaggggcgcg cccggttgag atctggctgg cggcagagga tactccgcag accctgatga
     4981 gcgccgccgg gacgccaatt gaactattga ctgaagccgg g
//
LOCUS       DAOPVS010000142         5003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_16846_length_5003_cov_29.642684, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000142 DAOPVS010000000
VERSION     DAOPVS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16846_length_5003_cov_29.642684"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="PKL11_09345"
     CDS             <1..1977
                     /locus_tag="PKL11_09345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ13847.1"
                     /translation="ESIRNAIEALGWATASTHVSIFENAINADGRVYPNYRYEWVAPG
                     AVNASDNDQFFIDAARSGFGRWADLLSRNKIVKARASLSPTGSLFRGSKSILVVPIFV
                     FTRWWGGICVADALHEREWTTGEEDALRLAASLVGLAIQRQHYDNQNRQLLAAESSAR
                     KLAEALGKSAADLNASLSLDSVLDSIMANIGKVTAHDAVNIMLVDNGRAEVLRAQGYQ
                     RRSNSGNNLPVHAVMNTPVMRQAAESGRPVIVAEAAAVIHQQGSEHMQWVRSLATIPI
                     QHQGATIGFINLQSAAPNAFSANQADTLQAFANQAALALQNARLFETVRENAHRLSIL
                     NEIGRIAISAPSLEALLDGLANRVQSLFGMDAAYITLWDEANQRPIPASASGQQGAAY
                     SATEVVNGEITLTESVLNARRVLIVEDAYHSSYISSRIARHFNEASIMGIPLIVGDQK
                     LGALIMGYTQPHKFTPDEAALAEQAGRQIALAVAKAELVAEVQRLAVTDDLMGLYNYR
                     GLMDLGAQEVDRAQRYGRPLSAIMMDIDRFKTLNDTYSHIVGNEILCQLADRCRQVVR
                     DADIVTRYGGDELCILLVETDDVTALHIAERIRQSVAGAPFLVSVGAIPATVSLGVSS
                     LKPGETLNQLIARADRGLYHAKEGGRNRAVFEAD"
     gene            complement(1974..>5003)
                     /locus_tag="PKL11_09350"
     CDS             complement(1974..>5003)
                     /locus_tag="PKL11_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNZ13848.1"
                     /translation="SRGRVLNYYGYVLNTNATRETEEALRKSEERNRAILNAIPDMMF
                     RVNREGTFLDYHAERTEHLIMPPDLFLGKRVVDVLPPELAQLTMKSVRLALNTRKIQT
                     YEYSLNMGKEDRFYESRLMVSGPDEVLAVVRDITQQKMAQNKQLEAEERYRQLVEQIT
                     AITYIDSADGTQTAYYISPQIHTLLGYRPEEWMAEERIWRRLIHPDDREWVLNEVDRC
                     SQAGKEIAMEYRLVAKDGHVVWVRDEAVMSRDKAGKPIAWRGMMLDITARKQAELMQA
                     AAFQISQAALTSTSLEELFPSIHQTLKALLPTDNFYIAIYEPKNDLLSFPYWADVKDE
                     KPNPAPPGKGLTEYVLWTGKSLLVSPDRYRELIKAGDVEQIGAESVDWLGVPLKADGG
                     TFGVLAVQSYNPTIRYTEREREILEFISVQVAMVIQRKQAEESLRESEQRYVLAVRGA
                     NDGLWDWNLRTDQIYFSPRWKAILGLNLDGVPDTPDLWFSRIHPEDIQRVMGQLTAHM
                     KGQTPHFECEYRALHDNGSYRWVLVRGLATRDEKQQAVRMAGSMSDITARKAIEEQLS
                     HDAMHDTLTGLPNRAYVIEQLQRCMEICRRRPTYLAALLLLDLDRFKVVNDSLGHLCG
                     DELLVLVGERIRDCLRPGDVVGRFGGDEFAVLLEDLHNPGEVAVVARRLLEALEKPFK
                     IEGREVFTSASIGITMGNGDYERPEDIIRDADTAMNRAKAKGRARYQIYDVEMHATSI
                     SQLELETDLRRAIERQELRVYYQPIISLQTRRITSLEALVRWQHPRRGLTLPGEFMPL
                     AEDTGLVLPIGEWVLKTALADLKIWREIGFKDLQVAVNVSVRQLESQDLIGLVKSALK
                     DTGLPAQALQLEITESAAMHDVEQTAKILDVLGKMGIDISIDDFGTSYSSLGYLNRFP
                     VRHLKIAQTFTRNIPDRRDDTAITHAIIALAHALKLTVIAEGVENERQLATLIPLGCE
                     YIQGYMISRPQPADAIVQVLNESPKNLKIRELPSQLN"
BASE COUNT         1056 a         1545 c         1242 g         1160 t
ORIGIN      
        1 gaaagcatcc gcaacgccat tgaagctctg ggttgggcca cagcttccac ccacgtctcc
       61 atttttgaaa acgccatcaa cgcagatggc cgcgtctatc ccaactatcg ctatgaatgg
      121 gtggcgcctg gtgccgtaaa cgcctcagat aacgatcagt ttttcattga tgcagcccgc
      181 tctggctttg gacgctgggc cgatctgtta tctcgcaata aaattgtcaa agcccgcgcc
      241 agtctctcgc caaccgggtc gttgttccgg ggcagcaaat ctatcctggt ggtccccatc
      301 ttcgtcttta cccgctggtg ggggggcatc tgtgtggccg atgccctgca tgagcgcgaa
      361 tggaccaccg gcgaagaaga cgccctccgc ctggctgcca gtctggttgg cctggccatc
      421 cagcgccagc attacgataa tcagaaccgc cagcttttgg ctgccgaaag cagcgcccgc
      481 aaactggccg aagccctggg taaatcggcg gcagatctca atgcctccct gagcctcgac
      541 agtgtattag acagcatcat ggcaaacatt ggcaaagtca ccgcacacga tgccgtcaat
      601 atcatgctgg tggataatgg ccgcgccgag gtgctccgtg cccagggtta tcagcggcgc
      661 tccaattcgg gcaataacct gccagtccat gccgttatga acacacctgt catgcgccag
      721 gctgccgaat cgggccgccc ggtcattgtg gcagaggccg cggctgttat ccaccagcag
      781 ggctctgagc atatgcagtg ggtgcgctca ttggccacca tcccgattca acatcaaggc
      841 gctaccattg gctttattaa ccttcaaagc gctgcaccca atgcattttc tgccaatcag
      901 gccgatacgc ttcaagcctt cgccaaccag gcggccctgg ctttgcaaaa cgcccgcctg
      961 tttgagacgg ttcgtgagaa cgcccatcgc ctctcaatct tgaatgaaat aggtcgtata
     1021 gccatatcgg cgccctcact ggaagccttg ctggatggtc tggctaaccg ggttcaatcc
     1081 ctctttggga tggatgccgc ctacatcaca ttatgggatg aagccaatca gcggcccatc
     1141 cccgcttctg cctctggtca gcagggggca gcctattcag ccacagaagt ggtgaacggc
     1201 gaaatcacgc tgaccgaatc ggtactgaat gcccggcggg tcctgattgt ggaagatgcc
     1261 tatcactcat catatatcag ctctcggatt gctcgtcatt ttaatgaagc ttcaatcatg
     1321 ggaattcccc tgattgttgg tgaccaaaaa ttgggtgctt tgattatggg ctatacccag
     1381 ccccataaat tcacccccga tgaagccgct cttgctgaac aggccgggcg gcagattgct
     1441 ctggctgttg ccaaggccga gctggttgcc gaggttcagc gcctggccgt cactgatgat
     1501 ttgatgggtc tgtacaacta ccgcggcctg atggatctcg gcgcccagga ggttgaccgc
     1561 gcccagcgtt acggccgtcc tctatcagcc atcatgatgg atattgaccg cttcaagacc
     1621 ctgaacgaca cctattccca tatcgtcggc aacgaaatct tatgccagct tgccgatcgc
     1681 tgtcgccagg tggtgcgtga tgccgatatc gtcacccgtt acggtggcga tgagctttgc
     1741 atccttctgg ttgaaacgga tgacgttacc gccttgcata ttgccgagcg cattcgccag
     1801 tcggtcgccg gagcgccgtt cctggtcagt gtaggcgcca ttccggcaac agtcagcctg
     1861 ggagtctcct ccctcaaacc gggcgaaacc ctcaatcagc tcatcgcccg tgccgaccgt
     1921 ggcctctatc acgccaaaga gggcggacgc aaccgggccg tctttgaagc cgattaattc
     1981 agctgggatg gcagttcccg gatttttaaa tttttggggc tctcattcaa aacttgcaca
     2041 atcgcatcgg ctggctgtgg ccggctgatc atataacctt ggatatactc gcatcccagc
     2101 gggatcagtg ttgccagttg ccgctcattt tcaacccctt cggcaatcac ggtcagcttc
     2161 agcgcatggg ccagtgcaat aatcgcatgg gtaattgcag tgtcatcgcg ccggtccggg
     2221 atgttccggg taaaggtttg ggcaatcttc aagtgtctga ccgggaagcg gttcaggtaa
     2281 cccagcgatg agtagctggt tccaaaatca tcaattgaaa tatcaattcc cattttcccc
     2341 agcacatcca ggatcttggc cgtctgttcc acgtcatgca ttgccgcgct ctcggtaatc
     2401 tccaattgca gtgcttgtgc cggtaatcca gtatctttaa gcgccgattt taccagccca
     2461 attaaatcct ggctctccaa ctgccgcaca gatacattca cggcaacctg caggtcctta
     2521 aaccctatct cacgccagat ctttaaatca gccagcgcag tttttaaaac ccattcccca
     2581 atcggcagca ccagccccgt gtcttcagcc aggggcataa attcaccggg cagtgtcagt
     2641 ccgcggcggg ggtgctgcca gcgtaccagc gcctccagcg aggtgatccg ccgcgtttgc
     2701 agcgaaataa ttggctggta atacacccgc agttcctgcc gctcaatcgc tcgccgcaaa
     2761 tcggtttcca gttcaagctg cgagatggag gttgcatgca tctccacatc ataaatctga
     2821 tagcgcgccc ggccttttgc cttggcccgg ttcatagccg tatccgcatc ccggataata
     2881 tcttccgggc gctcataatc gccattcccc atggtgatcc caattgaggc cgaggtgaac
     2941 acctcccgcc cttcaatctt gaatggcttc tcaagagctt ccagcagccg ccgggccacc
     3001 acggccactt cgcctgggtt atgcaaatct tccagcagca ccgcaaactc atcgccgccg
     3061 aatcgcccaa ccacatcacc agggcgcagg cagtcccgaa tccgctcgcc aaccaatacc
     3121 agcagttcat cgccgcacaa atgccccagg ctgtcgttca caaccttgaa tcggtccagg
     3181 tccagcagta ataatgctgc caggtaggtt gggcggcggc ggcaaatttc catgcagcgc
     3241 tgcaactgct caatcacata agcccggttg ggcagccccg tcagcgtatc gtgcattgca
     3301 tcgtggctta gctgctcctc aatggctttg cgcgccgtaa tatcgctcat tgagcccgcc
     3361 atgcgcacag cttgctgctt ctcatccctg gttgccagcc cgcgcaccag cacccagcgg
     3421 tacgaaccgt tgtcgtgcag cgcccgatat tcgcactcaa agtgcggcgt ctgtcccttc
     3481 atgtgggcgg tcaattgccc catcacgcgt tgaatatctt ccgggtgaat gcggctgaac
     3541 cacaaatcgg gtgtatctgg tacgccatcc aggttcaagc ccagaattgc cttccagcgt
     3601 ggcgaaaaat aaatctggtc cgtgcgtaaa ttccaatccc ataacccatc attggcgccg
     3661 cgcaccgcca gcacatagcg ctgttcactc tcacgcagtg attcctccgc ctgctttctt
     3721 tgaatcacca tcgccacctg aacagagata aactctaaga tttcccgctc ccgctcagta
     3781 tagcgaatgg tcgggttata gctttgcacc gccagaaccc caaaggtccc gccgtctgct
     3841 ttcaggggca cccccagcca gtccaccgat tcagccccaa tttgctccac atcacccgct
     3901 ttgattaatt ccctgtaccg atcaggcgat accagcagcg acttgcccgt ccagagcacg
     3961 tattccgtca ggcctttgcc cggcggcgcc gggttgggct tctcgtcttt cacatcagcc
     4021 cagtacggaa acgacagcag atcgttcttt ggctcataaa tagcaatata aaaattatca
     4081 gtgggcagca gcgccttaag tgtctgatgg atggatggga atagctcttc cagcgaggtg
     4141 gaggtcagcg ctgcctgtga tatctggaac gccgccgcct gcatcaattc tgcctgtttg
     4201 cgcgccgtaa tatccagcat catgccgcgc cacgcaatcg gcttgcccgc cttgtcgcgc
     4261 gacatcacgg cctcatcccg tacccagacc acatgcccat cttttgccac cagccggtat
     4321 tccatggcaa tctctttacc tgcctggctg caccggtcca cctcattcag cacccattca
     4381 cggtcgtccg gatgtatcaa cctgcgccag atgcgctctt cagccatcca ttcttcgggg
     4441 cgatacccca agagcgtatg aatttgtgga ctgatgtaat aggcggtctg ggttccatca
     4501 gccgaatcga tgtaagtgat cgccgtgatt tgctcaacca gctggcggta ccgctcttca
     4561 gcttcaagtt gtttattctg agccattttt tgctgggtga tgtcgcgcac aaccgccaga
     4621 acctcatccg gcccgctgac catcaaccgg gattcataaa accgatcttc tttccccata
     4681 ttcaaagaat actcataggt ctgtatcttg cgagtgttaa gcgccagacg cacagatttc
     4741 atcgtcagtt gtgccagttc gggaggcagc acatccacaa cccgcttgcc caggaataaa
     4801 tcaggcggca tgatcaggtg ctcagttcgc tcagcatgat aatccaggaa agtcccctcc
     4861 cggttgaccc tgaacatcat atcggggatt gcattcagaa ttgcccggtt acgttcctcg
     4921 ctcttgcgca gtgcctcttc tgtttcacgg gtcgcattgg tattgaggac atagccataa
     4981 taattcaaaa cgcgcccacg cga
//
LOCUS       DAOPVS010000143         4913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_17255_length_4913_cov_57.793742, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000143 DAOPVS010000000
VERSION     DAOPVS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17255_length_4913_cov_57.793742"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKL11_09355"
     CDS             <1..234
                     /locus_tag="PKL11_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13849.1"
                     /translation="PQNPPNNNRRNKMPDQKPPSKNRQPQGKEWISLGVGMGLALGAG
                     IGVAMDNLAVGIGIGLVIGAAVGVALSQRKAQP"
     gene            complement(308..1027)
                     /locus_tag="PKL11_09360"
     CDS             complement(308..1027)
                     /locus_tag="PKL11_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNZ13850.1"
                     /translation="MIQIKNVHKYFGHVHALQGVSLEIARGEVVVIIGPSGSGKSTML
                     RCINRLEEFDEGQIIVDGIPLDTAANINAVRTEVGMVFQQFNLFPHLSVLENLALAQR
                     IIRKRSRAEAESVAMDLLKKVGIPDKAAAYPLQLSGGQQQRVAIARALAMNPKIMLFD
                     EPTSALDPEMIQEVLDVMMTLAKEGMTMVVVSHEMGFTRAAANRAILMDAGQIIEQGP
                     PSELFTNPKEERTKLFLSKIL"
     gene            complement(1038..2108)
                     /locus_tag="PKL11_09365"
     CDS             complement(1038..2108)
                     /locus_tag="PKL11_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNZ13851.1"
                     /translation="MELIALVFFAQYIGKVVRAKGYKPWPYILMTVGMWIFGEALGAW
                     NAIILFGTQAFKMESGPAWLGTALKFGLIYLFMLLLAAAAALAAKTIAKNLRPVGSTE
                     AIKKMDIWWLLVLAADLILLILATVQPDPFQRIVTFVLDGLWVTIYVTLISFVLVLFF
                     GLLAGLARLSRIGIIRSLATIYVEVIRGIPILVQLMFWYFAFPAILKSLGAAWDWAIL
                     ADYKANPLVMAILGLTVAYSAYMSEVYRAGIQSIAKGQMEAARSLGMTYFQAMRYIIL
                     PQAVRVILPPVGNEFITLLKDSSLVSVVAVADMTRRGREFQASNFMPIEPWIIVGLLY
                     LMMTLFSARVVTWLEHKTRLER"
     gene            complement(2195..3016)
                     /locus_tag="PKL11_09370"
     CDS             complement(2195..3016)
                     /locus_tag="PKL11_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ13852.1"
                     /translation="MKRFALITLMALMVLSLALTACTPKPTEVVDTGYPAPETNAVIP
                     AGYPAPGLKVVVVTDATFPPFETVDEATKDLTGFDIELMNAIAAKLGLEVEYVNTGFD
                     PALAGVASCQYDAAIAAITITDARKAEMLFSEPYINAGQVVVVAKDNTTINSKDDLDG
                     LTVAAQLGTTGEIEAQAIPNVTYKPYDTYDLALLDLANGQVDAVIADYPTALGFIGQN
                     PDDLKTVGPVFTNESYGIAVCNSKPELVGYFNLALAALKADGTISNLEQKWLAGQ"
     gene            complement(3200..4894)
                     /locus_tag="PKL11_09375"
     CDS             complement(3200..4894)
                     /locus_tag="PKL11_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNZ13853.1"
                     /translation="MKPHSALILALIFILITGCTAGPTPAPTLTRPPAPTATPTPAQP
                     AATATPTLTPQPSPTPNHTQLPLTLTLMGISVTGAFILDANSTYFIGVESVPDASGYR
                     WAISQNGELVFDSYAAGRLAGSSFYISRDSVPLSRLTTGAPADLLVQARIGEQWSTPT
                     RRTIYFFASQDEYAAWPTPAPVIATPLPAASTEFGGAKIVFSDSAMVYSINADGFNQV
                     RLANVGQPDALSVSDDGRLAAFSAAGAAIYAVNTDGTDLRPLGIQGDVVGGAALSPNG
                     LWVAYMSNAAGRHQVHRQSWDFDLWVTSTTDSSQYRLVESTASVEWPAWSPDGTTVAY
                     VLLEDQGSEQAPQILPTLYTVKINTGEKMPVAQLPLGWVTGLTWSPDGRYLAFVSTGA
                     GGAPRLYLIRANGLDLLPLTEAVMDAQAAPAWLPDSRHLYFDGSILDILTGEAVSFEP
                     AAVQALRASGARLAGWSDPQGDGAAVDCTQGWTHLAVGGTATVNTASNMPNRVRSGPS
                     RSAAVITQILPGVTVRLLAGPVCADGLIFWQVESAEIPGGVGWTAEGDGVDHWLVP"
BASE COUNT          943 a         1468 c         1625 g          877 t
ORIGIN      
        1 ccgcaaaatc cccccaacaa caacaggaga aataagatgc ccgaccagaa gcccccctcg
       61 aagaacagac agccccaggg caaagaatgg atcagcctgg gcgttggcat gggcctcgcc
      121 ctcggagcag ggattggcgt ggccatggat aacctggccg ttggcattgg cattggcctg
      181 gtcattggcg cggcggtggg cgttgcgctc agccagcgca aagcgcagcc gtagcgctgg
      241 aatgcaaagg cgcaaaggat ttagcatcct ttgcgccccg gtttgcttga cggttgaagc
      301 aggcggctta caaaatcttg ctcaggaaga gcttggtgcg ttcttccttg gggttggtga
      361 agagttcgct cggcggcccc tgctcaatga tctgcccggc gtccatcaaa atggcgcggt
      421 tggccgcggc gcgggtaaag cccatctcgt ggctgaccac caccatcgtc atgccttctt
      481 tggccagcgt catcatcaca tccagcactt cctgaatcat ctcggggtcc agggcgctgg
      541 tgggctcgtc aaacagcatg atcttggggt tcatggccag ggcgcgggca atggccacgc
      601 gctgctgctg cccgcccgaa agctgcagcg ggtaggcggc ggccttgtcg gggatgccga
      661 cctttttcag caggtccatc gccacgcttt cggcctcggc ccggctgcgc ttgcggatga
      721 tgcgctgggc cagggctaaa ttctccagca ccgaaaggtg cgggaagagg ttgaactgct
      781 ggaagaccat gcccacctcg gtgcgcaccg cgttgatgtt ggcggccgta tccagcggaa
      841 tgccatccac aatgatctgg ccttcgtcaa attcctccag ccggttgatg cagcgcagca
      901 tggtcgattt gcccgagccc gacggcccaa taatcaccac cacctcgccg cgcgcaatct
      961 ccagactgac gccctgcagc gcatggacgt ggccgaaata cttatgcaca tttttgattt
     1021 gaataatcgg ttccatgcta gcgctccaga cgggttttgt gttccaacca ggtgaccacg
     1081 cgggccgaga agagggtcat catcagatag agcaggccca caataatcca gggttcaatc
     1141 ggcataaagt tggaggcctg aaattcgcgg ccccggcggg tcatgtcggc cacggccacc
     1201 acactgacca gcgatgagtc tttgagcagg gtgatgaact cgttgcccac gggcggcaaa
     1261 atcacgcgca cggcctgtgg caggatgatg tagcgcatgg cctgaaaata ggtcatgccc
     1321 aggctgcggg cggcttccat ctgccccttg gcaatcgatt ggatgcccgc ccggtaaacc
     1381 tcgctcatat aagcggagta ggccaccgtc aggcccagaa tagccatcac cagcgggttg
     1441 gctttgtaat cggccagaat ggcccagtcc caggccgctc ccaggctttt gaggatggcc
     1501 gggaaggcga aataccagaa catgagctgc accagaatcg ggatgccgcg gatgacctcg
     1561 acatagatgg ttgccaggct gcggatgatg ccgatgcgcg acaggcgggc cagcccggcc
     1621 agcaggccga agaacagcac cagcacaaac gaaatcaggg tgacgtaaat cgtcacccac
     1681 aggccgtcca gcacaaaggt gacgatgcgc tggaagggat caggctgaac ggtggccagg
     1741 atcagcagaa tcaggtcggc agccagcacc agcagccacc agatatccat ctttttgatg
     1801 gcctcggtgg agccgacggg ccgcagattt ttggcaatcg ttttggctgc cagggcagcc
     1861 gcggcggcca gcagcagcat aaacaggtaa atgagcccaa acttgagggc tgtgcccagc
     1921 caggccgggc cgctttccat tttgaaggct tgcgtgccga ataaaataat ggcgttccag
     1981 gcccccaagg cttcgccaaa aatccacatg cccaccgtca tcaagatgta gggccagggt
     2041 ttgtagccct tggcgcgcac caccttgcca atatactggg cgaagaagac cagagcaatt
     2101 aattccatgc cgcacctgcg cttcccagtc aaatgccggc ctgcggggcc aggcgtgcca
     2161 ctccgcaggc cggtgaatta cgattaaggg cggtttactg acccgccagc cacttctgtt
     2221 cgaggttgct gatggtgcca tcggctttca acgcggccag cgccaggttg aagtagccca
     2281 ccagttcggg tttgctgttg caaacggcaa tgccgtaaga ctcgttggtg aagaccgggc
     2341 caaccgtctt caggtcatcg gggttctgac cgatgaagcc cagggcggtg gggtagtcgg
     2401 cgatcacggc atccacctga ccattggcca ggtcgagcag ggccaggtcg taggtgtcgt
     2461 agggcttgta ggtcacattg gggatggcct gggcctcaat ttcgccggtg gtgccgagct
     2521 gggcggccac agtcaggcca tccaggtcgt ctttgctgtt gatggtggtg ttgtctttgg
     2581 ccaccaccac cacctgcccc gcgttgatgt agggctcgga gaagagcatt tcggccttgc
     2641 gcgcatcggt gatggtgatg gcggcaatgg cggcatcata ctggcaggag gccacgccag
     2701 ccagggcggg gtcaaagcca gtgttgacgt attcaacttc gaggccaagc ttggcagcaa
     2761 tggcgttcat cagttcaatg tcgaagccgg tcaggtcctt ggtggcttca tccaccgtct
     2821 caaagggcgg gaaggtggca tcggtcacga cgacgacctt caggccgggg gcggggtaac
     2881 ccgccgggat gacggcgttg gtctcggggg cggggtagcc cgtatcaacc acttcggtgg
     2941 gcttaggcgt gcaggccgtc agcgccaggc tgagtaccat cagggccatc agcgtgatca
     3001 gagcgaaacg tttcatgtaa gatcctccat atgaaatttt ggaaacaggg gaagggcgac
     3061 ttctgcccgc cttgggcggg gcagataatt tgattatacc ccaaattatc cgccccgtcc
     3121 tttgcgtcgc ggcagggcgg ggtgggtttg gcccggcctc tgccgtggca cctggaatgg
     3181 ggaacgaacc cgaagcgccc tacggcacca gccagtgatc cacgccgtcg ccctcggcgg
     3241 tccaacctac gccgccgggg atttcggccg actccacctg ccagaagatc agcccatcgg
     3301 cgcagaccgg ccccgccagc agccggacgg tgacgccggg caaaatctgc gtgatgaccg
     3361 cagccgaccg gctggggccc gagcgtaccc ggttgggcat gttggaagcc gtgttgacgg
     3421 tggcggtgcc gccgaccgcc aggtgcgtcc agccctgggt gcagtccacc gccgcaccat
     3481 ccccctgcgg gtcagaccag cccgccagcc gcgccccgct ggcgcgcagc gcctgcactg
     3541 cggcgggttc aaagctcact gcctcgccgg tcaaaatatc cagaatggaa ccgtcaaaat
     3601 agaggtggcg gctgtcgggc agccaggctg gcgcagcctg ggcatccatc acggcctcgg
     3661 tcagcggcag cagatccaag ccgttggccc ggatcaggta gagccggggg gcgccccccg
     3721 cgccggtgga gacgaaggcc aggtagcgcc catcgggtga ccacgtcagc ccggtgaccc
     3781 agcccagcgg gagctgcgcc acgggcatct tttcgccggt gttgattttg acggtgtaga
     3841 gggtgggcaa aatctgcggg gcctgctcgc tgccctgatc ttccagcagc acataggcca
     3901 ccgtggtgcc atccggcgac caggccggcc actcgacgga ggcggtgctt tccaccagcc
     3961 ggtactgcga tgaatcggtg gtgctggtca cccacaggtc aaaatcccag ctctgccggt
     4021 gcacctggtg ccgcccggcc gcgttggaca tataggccac ccacaggccg tttggcgaga
     4081 gcgccgcgcc gcctaccaca tcgccctgaa tgcccagcgg gcgcaggtcg gtgccatcgg
     4141 tgttgacggc gtaaattgcg gcgcccgccg cgctgaaggc cgccaggcgg ccatcgtccg
     4201 agaccgacag tgcatcgggc tggcccacgt tggccagccg gacctgattg aagccatcgg
     4261 cgttgatgga atagaccatg gcgctgtctg aaaagacgat ttttgcgccg ccaaattcgg
     4321 tgctggccgc gggcagcggc gtggcaatga ccggcgccgg ggtgggccag gcggcatatt
     4381 catcctgact ggcaaagaag tagatcgtcc gccgggtggg ggtggaccac tgctcgccga
     4441 tgcgggcctg caccagcaga tctgccgggg cgccggtggt caggcggctc aacggcacgc
     4501 tgtcgcgcga gatgtagaag gatgatccag ccaggcggcc agcggcatat gaatcaaaaa
     4561 ccagttcgcc gttctgactg atggcccagc ggtagccgct ggcatctggc acactctcca
     4621 cgccgatgaa gtaggtgctg ttcgcgtcca ggatgaaggc gcccgtcacg ctgatgccca
     4681 tcaacgtgag cgtgagcggg agctgggtgt ggttgggcgt gggcgaaggc tggggcgtga
     4741 gggtgggggt ggcggtggca gcgggctgcg ccggcgtggg ggtggctgtt ggcgcgggcg
     4801 gccgggtgag ggtgggcgcc ggggtgggcc ccgccgtgca gccggtgatg aggatgaaaa
     4861 tcagggccag aatcagggca gaatggggtt tcatgggcag gtctcctcgt cca
//
LOCUS       DAOPVS010000144         4911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_17262_length_4911_cov_35.771005, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000144 DAOPVS010000000
VERSION     DAOPVS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17262_length_4911_cov_35.771005"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKL11_09380"
     CDS             <1..245
                     /locus_tag="PKL11_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13854.1"
                     /translation="KARLDLAQQAARADNFQLYFDLRDELETKLKDGLRPPKTAAKAL
                     SGEAEQVHGTRPLTDATTPALEGEELWRTLRYVGRT"
     gene            401..784
                     /locus_tag="PKL11_09385"
     CDS             401..784
                     /locus_tag="PKL11_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ13855.1"
                     /translation="MTRTHSPTRCEENVIHEEAVRAAQTRLVDGLTATHLAQTFQALS
                     DPARVRLISALLENELCVCDLAAVLGMSQSAVSHQLRSLRDLHLVRTRKAGRIVYYAL
                     DDAHIRDLFQRGLEHIQHHQQGSAA"
     gene            781..2787
                     /locus_tag="PKL11_09390"
     CDS             781..2787
                     /locus_tag="PKL11_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNZ13856.1"
                     /translation="MTSPNSVENSACGCAACETTATRTARKPLNFFSFLWARPEARLA
                     LGAGLLLGIAVLIEALGGPLLLVRALQIAALSIAGYPVARSGLLGLIKEHEFNINLLT
                     SVAALGAIFIGEWTEAATLMFLFAIAEALEDYAVARSRSALGELDALLPETALRLVNG
                     QPEVVSVNDLAVGDRLLVRPGERVPMDGQVVAGQSEVNQAPITGESLPVEKVTGDEVF
                     AGTVNGSGALEIEVTRRVTENTLHRIIEMVEEAQARRAPVQRIIDRFARVYTPAVMVL
                     AALTAALPPLLFGAPFLDTPDGRGWLYRALALLVISCPCALVISAPATILSGITAAAR
                     QGVLVKGGAFIEALAQVRLFAFDKTGTLTLGRPVVLQARCPNCPDGAHCADCDQVLAL
                     AAALEQRSAHPLAQAVINAADARGLAGAYAPAEAVTTLAGLGLRGEINGSPVTVGSHR
                     LFEAEHPHPAQFCAWVEQAERQGQMAMLVADGADVRGLITAADELRPESPAVIAALHE
                     LGCQTALLTGDNPGAAQSVAARVGIDTVNARLLPADKLAAIAELRAAGPVAMVGDGIN
                     DAPALAAADVGIAMGGAGSAQAMETADVVLMADGLAQLPGAVRLARQVRMLIKQNIAF
                     SLLAKLAFMALALGGITTLWLAVLADTGITLLVTANGMRPLARR"
     gene            complement(2921..>4911)
                     /locus_tag="PKL11_09395"
     CDS             complement(2921..>4911)
                     /locus_tag="PKL11_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13857.1"
                     /translation="NYPISIISAFLPALERSPFILPNLLSIKHDILSRLEANILQGVA
                     MTLDQLPLISWLKLGAAFLIFLGPGFLTLQFSSFRKELDFSFILVAAFGLSLGEWTVF
                     LGLTNLLGLHTLPAIAISLFLLCWVAGLLVQKPWQHSFKKSPPFKLDRIALWLILTIG
                     FVISLWGICNEVAGLGSDSYHHTLITQMIMDQGRLPQDYGSFLPIISFRYHYGFHALA
                     AFLGWVSGIPARLLVLALGKVLISLCAAAVALATEKISGRRAAGLMATVIVAVTCVFP
                     AYMLTWGRYTQLTGLVILALFLAFFWLWIKRDYNTGNILVLAVLAAGMGLTHYRIVAM
                     AVTGVLILAMVEGLGRHSWKDWQRIAFRGLLLMFVVLALTAPWLIHLGVTHQIGFPVV
                     AAERTDNYFSLSRFSTELKQYPTNIPMLILATLAMLSGWIRRDRVTIGLTLWFVVLLL
                     PSRYILLMDTTSVFISVFLPAAIVIGQEGNNLIDAIAHFFARPRLRQILASTFILLLL
                     IGGLAANLVYPIVNDGLLHPVDLPAMEFIRQETSSDAYFMVNMFHFPFSDTLVAGSDA
                     GAWIPLLAQRRVTLPVLNYYIERLNDPTWVDRLCQLDQLNGDITSEAGLALLRAEGIT
                     HVYIGDRGGVIQPASLLSSPDFNLIYQDQTVYIFELIK"
BASE COUNT         1142 a         1308 c         1482 g          979 t
ORIGIN      
        1 tgaaggcccg gctggacctg gcgcagcaag ccgcccgcgc cgataacttc cagctttatt
       61 ttgacctgcg ggatgaactg gagaccaagt taaaggatgg tctgcgtccg cccaagacgg
      121 ccgccaaagc cctgagcggc gaggctgagc aggtgcacgg cacccgccca ctgaccgatg
      181 caaccacccc tgcgttggaa ggcgaggaac tgtggcgcac actgcgctat gtgggcagaa
      241 cgtaagaaaa gttagcttgc gaatttgaag aacccggcgg tcatggcttt attgcctgac
      301 catcgggttc tttgatttat caggcgctca cgcgcttgac atctcttcag ccggcggcta
      361 taatacatga atacatgttc atatattcag gaataagccg atgacccgaa cgcactctcc
      421 cacccgctgt gaagagaatg taatccatga agaagctgtg cgcgcggcac agacgcgcct
      481 ggtggatggc ctgacggcca cacacctggc acagaccttt caggcgcttt cagacccggc
      541 gcgggtgcgg ctcatctctg cgctgttgga gaacgagctg tgcgtgtgtg acctggctgc
      601 cgtgctgggc atgtcacaga gtgcggtttc gcaccaactg cgcagcctgc gcgacctgca
      661 cctggtgcgc acccgtaaag ccgggcggat cgtctactat gcgctggatg acgcccatat
      721 tcgcgacctg tttcagcgcg ggctggaaca tattcaacat caccaacaag gatcagcagc
      781 atgacctcac ccaattcggt cgaaaattct gcctgcggct gtgccgcctg cgaaacaaca
      841 gccacccgca cagcccgcaa accgctcaac ttttttagct ttctgtgggc gcggcccgag
      901 gcgcgtctgg cgctgggcgc cgggctgctg ctgggcatcg ccgtgctgat tgaagccctg
      961 ggcgggcctt tgctgctggt gcgggcgctg caaattgcgg cgttgagcat tgccggttac
     1021 ccggtggcgc gcagcggact gctgggattg attaaagagc acgagttcaa catcaacctg
     1081 ctgaccagtg tggccgcgct gggggcaatc ttcatcggcg aatggacgga agcggctaca
     1141 ttaatgttcc tgtttgccat tgccgaggcg ctggaagatt acgccgtggc gcgcagccgg
     1201 agcgcgctgg gtgaactgga cgcgctgctg cccgaaaccg ccctgcggct ggtcaatggt
     1261 cagcccgagg tcgtctcggt caacgatctg gcggttggcg accggctgct tgtccgcccg
     1321 ggcgagcggg tgccgatgga tgggcaggtg gttgccgggc agagtgaagt caaccaggcg
     1381 ccgattacgg gtgagagcct gccggtggaa aaagtcactg gcgatgaggt ctttgccggg
     1441 acggtgaacg gcagcggcgc attagaaata gaagtaaccc ggcgggtgac ggagaatacc
     1501 ctgcaccgga ttattgagat ggtggaagaa gcccaggcac gccgggcgcc cgtgcaacgg
     1561 attattgacc ggtttgcacg cgtctatact cctgcggtaa tggtgctggc ggcgctgacc
     1621 gcggcgctgc cgccgctgct ctttggcgcg ccgtttttag atacgcctga tgggcgcggc
     1681 tggctgtacc gcgccctggc gctgctggtg atctcctgcc catgtgcgct ggtcatcagt
     1741 gcgccggcaa ccatcctcag cggcatcacg gcggcggcgc ggcagggggt gctggtaaag
     1801 ggcggggcgt tcattgaggc gctggcgcag gtgcggttgt ttgcttttga caagaccggt
     1861 acgctgacgc tgggccgccc ggtggtgctg caagctcgct gccccaactg tccagatggg
     1921 gctcactgcg ccgattgtga ccaggtgctg gcgctggctg cggcgctgga acagcgcagc
     1981 gctcacccgc tggcacaggc agtcattaat gccgctgatg cgcgcgggct ggcaggggcc
     2041 tatgccccgg ctgaggccgt gaccaccctg gctgggctgg ggctgcgcgg cgaaatcaac
     2101 ggcagcccgg tgacggtggg cagccaccgg ctgtttgaag ccgaacaccc ccatccggcg
     2161 caattctgcg cctgggtgga acaggccgag cggcagggac agatggccat gctggtagct
     2221 gatggcgctg acgtgcgcgg gttaatcacc gcggcagatg aactgcgccc ggaaagcccg
     2281 gcagtcattg cggcgctgca cgagctgggc tgccagaccg ccctgctgac gggcgataac
     2341 cccggtgcag cccagtctgt ggctgcgcgg gtggggattg atactgtgaa cgcccggctg
     2401 ctgcctgccg acaagctggc ggcaattgcc gagctgcgcg ccgccggacc ggtggcgatg
     2461 gtgggcgatg gcatcaatga tgcgccggca ctggctgcgg cagacgtggg cattgccatg
     2521 ggcggggcag gcagcgccca ggccatggaa acagccgatg tggtgttgat ggccgatggt
     2581 ctggcgcagc tccccggtgc agtacggctg gcccgccagg tgcggatgct gatcaagcag
     2641 aacattgcct tcagcctgct ggccaaactg gcttttatgg cgctggctct ggggggcatt
     2701 accacgctgt ggctggctgt gctggctgat acgggcatca cgctgctggt caccgccaat
     2761 ggaatgcggc cgctggcgcg gcggtaggct ggaggtgggg ttttggatag aaggagcggc
     2821 ggatttttgt ctgccgcttc ttttgctttc aggtgtgaat gaccgccgtc acaagttttg
     2881 tgaagatggt gtcgaagggg ttagcgccgg ggaggaagtt ttatttgatt aattcaaaaa
     2941 tatagaccgt ctgatcttga taaatcaaat tgaaatctgg tgaggacagt aatgaagccg
     3001 gctggatgac cccgccgcga tcgccaatgt aaacatgagt gatcccttca gcgcgcaaca
     3061 atgccaggcc tgcttcagag gtaatatcgc cattaagttg gtcgagctgg cagaggcggt
     3121 ccacccaagt aggatcatta agacgctcga tataatagtt aagcactggc aatgttaccc
     3181 ggcgctgcgc cagcaatggg atccaggctc ctgcatcact tcctgcaacc agagtatccg
     3241 agaaggggaa gtggaacata ttgaccatga aataggcgtc ggacgacgtc tcttgccgaa
     3301 taaattccat ggctggcaaa tcaaccgggt gtaataatcc atcattgaca atcggataaa
     3361 caagatttgc tgcaagcccg ccaatcagta agagcagtat aaatgttgag gcaagaattt
     3421 gcctcaacct ggggcgagca aagaaatggg caatagcatc gattagatta ttcccctctt
     3481 gcccaatcac gatggcagcc ggcagaaaaa ctgatatgaa gacactggtc gtgtccatca
     3541 ggagaatata gcgtgaagga agcagcaaca ctacaaacca gagcgtcaaa ccaattgtca
     3601 cccgatcacg gcgaatccag cctgacagca tggctagagt tgccagaata agcattggga
     3661 tgtttgtagg atattgtttt agctctgtgc tgaatcggct cagtgaaaaa taattgtcag
     3721 ttcgttcagc cgcaacaaca gggaatccga tttggtgggt aacacccaga tggatcaacc
     3781 acggggccgt tagcgctaag accacaaaca tcagcagcaa accccgaaac gcaatacgct
     3841 gccaatcttt ccaagaatgc cgtcccagac cttcaaccat cgccagaatc agtacacctg
     3901 tgaccgccat ggcgacaata cggtagtggg tcagccccat gccggctgcc aaaacagcca
     3961 gaactagaat attgcctgtg ttatagtcac gttttatcca gagccaaaag aaagctaaaa
     4021 acagagctaa aattactaaa ccagttaatt gagtatatct gccccaagtc agcatgtatg
     4081 cagggaacac gcaagtgact gcaacaataa cagtggccat caacccggca gcccgccgac
     4141 ccgatatttt ttctgttgcc agagcaactg cagcagcgca taaactgatc aaaaccttcc
     4201 cgagcgccaa aaccagtaac cgtgctggta tgccgctgac ccaacccagg aatgcagcca
     4261 gagcatgaaa tccataatga tatcgaaagg agattattgg caagaaagag ccataatctt
     4321 gaggcagtcg accctgatcc ataatcattt gagtaattaa ggtatgatgg tagctatcac
     4381 ttcccagacc agccacctca ttgcaaatgc cccaaaggct gatgacaaac ccgatggtta
     4441 aaattagcca aagtgcgatt ctatctaact tgaagggggg actctttttg aaagaatgct
     4501 gccagggttt ctggaccaga aggccagcaa cccagcaaag taggaatagg ctgatagcaa
     4561 ttgcaggtaa agtgtgaagc ccaagcagat tggtcaaccc caagaagacg gtccattcgc
     4621 caagtgagag gccaaacgcg gccaccaata taaaactaaa gtctaattct ttgcgaaagc
     4681 tggaaaattg cagcgttaaa aagccagggc ccaagaaaat caagaaagcg gcgccgagtt
     4741 ttaaccagga gatcagaggg agttgatcaa gagtcatggc tacaccttgc agtatatttg
     4801 cctccagccg cgaaagtata tcatgtttga tactcaagag attaggtaag ataaagggtg
     4861 atcgttccag agctggtaaa aaggccgata taatggatat ggggtagttg t
//
LOCUS       DAOPVS010000145         4869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_17480_length_4869_cov_41.377649, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000145 DAOPVS010000000
VERSION     DAOPVS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17480_length_4869_cov_41.377649"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PKL11_09400"
     CDS             <1..827
                     /locus_tag="PKL11_09400"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ13858.1"
                     /translation="WYRTGEILKSRPGKTQVEDLPEVSRGVVPVMHCVQEIPCNPCTA
                     LCPHGLIYINKQDIRQIPTFLGNSDCCEVCERCVAGCPGLAITLVDYRGSNAEHPIVS
                     IPYEFTRDVIKAKDKVTVLDIEGAPLGEVEVVSIHTIPTSDRTLVVQVEAPKEIARQI
                     AGIRIQEAEITKPMDEYIEHIEDDIIVCRCERVTAGDIRALIRSGYRDINEIKTVTRA
                     GMGACGSKTCPAIIHRLFREEGIPVDEIVEVTKRPIFVEVPLSVFAGAEDSETSHE"
     gene            820..1989
                     /locus_tag="PKL11_09405"
     CDS             820..1989
                     /locus_tag="PKL11_09405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ13859.1"
                     /translation="MSEKKIPHYDVIVVGAGSIGTPTSLFLAKAGYKVLVLDPFASVG
                     QGSNKKAIGGIRATHSDPAKVRLCQRSIEIFRTWKEETGDDIEWYQGGYAFVAYREQE
                     EKALKNLVEKQKRDYQLNIDWLDHDAMLQVSPDLNPKGLIGGTFSPEDGSISPLLAVH
                     SFYTQAVAAGVTFHFDEKVTELVIEQGRVVGVRTPTASYSADVVINAAGPWAKEVGAM
                     AGVNVPVNPDSHEAAITEPVAHFLEPMIVDIRPAPGSSNFYFYQHLTGQIIFCITPSP
                     QIWGMDQRETSDFLPMVTRRMLEVMPRLKNIRVRRTWRGLYPMTPDGYPIIGWTKEAE
                     GFLLAAGMCGQGLMLGPGVAELVVRMVQNKMTPEDEHILSFVSPYRDFGGQEMLK"
     gene            2084..2740
                     /locus_tag="PKL11_09410"
     CDS             2084..2740
                     /locus_tag="PKL11_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ13860.1"
                     /translation="MSHLHGLVFDFDGLILETELPIFQSWQRVYEEYHAQLELSTWVR
                     CVGSSYEAFNPAIYLEEQTGLSIDRDALHQRAEIESNLIIDNQPPLPGVQQLLDEAKS
                     NGLKLAVASSSDSAWVRRHLKRLNLERYFDAICTKDDVEDVKPNPELYQLALERLGLQ
                     PEEAIAFEDSPNGIRAARAAGLRCIAVPNAVTQSLDFSLASAVIPSLAEVSIQKLNTF
                     "
     gene            2856..>4869
                     /locus_tag="PKL11_09415"
     CDS             2856..>4869
                     /locus_tag="PKL11_09415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNZ13861.1"
                     /translation="MKLEKLLGLLPENFSVIDREMIARAYHFAEDAHTGQKRAAGEAY
                     SSHCLATAVILAELRVSPTMIAAGLLHDVPLDTKSTSADIRREFGDEIGLLVDGVSRL
                     TTIPNITRADQHLEQIEAVNASLLQPAKTDPPTDGRRAEQNNEAARKLFLSMGDDVRI
                     VVVKLAEHLHNMRTLGALPDALRLRIAQQTLDVFAPLANRLGIWQIKWELEDLAFRHI
                     NPDKFKEISDKLADRREDRELQVRAIIEQLKKIMGDASIEADISGRPKHIYSIYRKMV
                     DKGKPFEMVRDLRGVRLIVNDVPTCYMALGIIHTRWRPIPNEFDDYIAVPKENNYQSL
                     HTAVIYDDGKPLEVQIRSREMHENAEYGIASHWRYKEKGLRTDQSYEQRVNWLRRLLE
                     WRSELEDSQDALDGMKGDVFQNRVYVFTPRGDIIDLPANATPIDFAYHVHTEIGHRCR
                     GARINGKLVGLDYCLKTGDQVEILTAKQGGPSRDWLNSALGLVRTSRARAKIRAWFVH
                     QDREHNLAQGKAMLERELKRLALTNVDLEKLAHTFDYPSSEELYVALGSGSLPIGRVV
                     HELSEEDQEQRDQIATGITGTLPSSAGLSVTGLKGLLTTIARCCNPAPGDEVIGYITQ
                     GRGATIHRVDCPNLPRLTEISPDRLIKVSWGETPRTYPVDIEIKAYD"
BASE COUNT         1213 a         1164 c         1396 g         1096 t
ORIGIN      
        1 cgtggtatcg gaccggcgag atcttgaagt cgcgacctgg gaaaacacag gtggaagacc
       61 tgccggaggt cagccgcggc gtagtcccgg tgatgcactg tgtgcaagaa ataccctgca
      121 acccctgcac cgcactttgc ccacatgggc tgatttacat caataaacaa gatattcgac
      181 agattccgac ttttttaggg aacagcgact gctgcgaagt gtgcgagcgc tgcgtggcgg
      241 gctgcccggg gctggctatc accctggtgg actaccgggg aagtaacgca gaacacccca
      301 ttgtctcgat cccctatgaa tttacaagag atgtcatcaa agccaaagac aaggtgaccg
      361 tgctggatat tgaaggtgcg ccccttgggg aagtggaagt ggtttccatt catacgatcc
      421 caacaagcga ccgaacactg gttgttcagg tggaggcgcc caaagaaata gcgcgccaga
      481 ttgccggtat tcgcattcaa gaagccgaga tcaccaagcc catggatgaa tatatcgagc
      541 atattgagga tgacatcatt gtatgccgct gtgaacgcgt tactgctggc gacatccggg
      601 cgttaattcg gtctggatac cgagatatca acgaaatcaa gaccgtaaca agggctggta
      661 tgggtgcctg tgggtctaaa acctgcccgg ccattattca ccggttgttc cgcgaggaag
      721 gcattccggt agatgaaatt gtggaagtaa caaaacgccc catttttgta gaagtgccgc
      781 tcagcgtttt tgccggcgca gaagattcgg agaccagcca tgagtgagaa gaaaatccca
      841 cactatgatg tcattgttgt tggggccggg tcaatcggca ccccaacctc tctgtttctg
      901 gccaaagccg gatataaggt tttagtttta gacccgtttg ccagtgttgg acaggggtca
      961 aacaaaaaag cgattggcgg tattcgcgcc acccattcag acccggcaaa agtgcgcctg
     1021 tgccagcgaa gtattgaaat tttccgcaca tggaaggaag aaaccggtga tgatattgag
     1081 tggtatcagg gcggatatgc ttttgtggct tatcgagagc aggaagagaa ggcgcttaaa
     1141 aacctggttg aaaaacaaaa acgagactat cagcttaata tcgattggct ggatcatgat
     1201 gctatgctgc aagtatcgcc agaccttaat ccgaaagggt tgattggcgg cactttttcg
     1261 ccggaagacg ggagcatctc gccattgctg gcagtgcatt cattctacac ccaggctgtg
     1321 gccgcgggcg tcaccttcca ctttgatgaa aaggtgacag agctagtgat tgagcagggc
     1381 cgggtggttg gcgtgcgtac accaacggca agctacagcg cagacgtggt gatcaacgcg
     1441 gcagggccct gggcaaaaga agttggggct atggcgggag taaatgtgcc ggtgaaccct
     1501 gattcgcacg aggcggctat taccgagccg gtggcgcatt tccttgaacc aatgatcgtg
     1561 gatatccgac ctgcgccggg atcttctaac ttttatttct accagcacct gaccgggcag
     1621 attatcttct gcattacccc cagtccgcag atctggggca tggatcaacg cgaaaccagt
     1681 gactttctgc ccatggtaac ccggcgcatg ctggaagtga tgccgcggct gaagaatatc
     1741 cgtgtgcggc gtacatggcg cgggttgtac cccatgacac cagacggtta cccgatcatc
     1801 gggtggacca aagaagccga gggcttcttg ctggcggcgg ggatgtgcgg acagggtttg
     1861 atgcttgggc cgggcgtggc tgagctggtt gtcagaatgg ttcagaataa gatgacacct
     1921 gaggatgagc atattttgag ctttgtgtcg ccgtaccgcg attttggcgg gcaggaaatg
     1981 ctcaagtaaa cacctcgaaa tatccgtgaa ttatgcggcg caccgggtat aataaatgcc
     2041 cggtgcgcct tgtggtaatt tttgcaccgg atggatgcgg tcaatgagtc atctgcatgg
     2101 gttggttttt gattttgatg gtctgattct tgagaccgag ctgccaattt ttcaaagctg
     2161 gcagcgcgtc tatgaagaat atcatgctca attagagttg agcacctggg tacgctgcgt
     2221 ggggtcatct tacgaggcct ttaatccggc tatctatctg gaagaacaaa ccggattgtc
     2281 gattgaccgg gatgcgctgc atcagcgagc tgagattgaa tccaatctga ttattgacaa
     2341 ccagccaccc ctgccaggag tacaacaact gctggatgaa gcgaaatcta atgggctcaa
     2401 actggcagtg gcttccagtt cagactctgc ctgggtgcgc cgccacttga agcggttgaa
     2461 tctcgagcgt tattttgatg ccatttgcac caaagacgat gtagaagatg taaagcccaa
     2521 cccagagttg tatcaattag ccctggagcg attaggactg cagcccgaag aagcaattgc
     2581 ctttgaggat tcgccgaacg gcatcagggc agcacgcgcc gctggtttgc gctgcattgc
     2641 ggttcccaat gccgtcaccc aaagccttga cttcagcctg gccagcgcgg tcatcccgtc
     2701 actggctgaa gtgagcatac aaaagctcaa tacgttttaa tcggcccctt gaattaaccg
     2761 atgctccaag tgtatacttg tccagatgat tgtttggtct tggtggtcac agatcagggc
     2821 cttgggcgat taatattttc caaacgagga cgcgtttgaa actcgaaaaa ctgcttggtc
     2881 tgctgcctga aaatttcagt gtgattgacc gcgagatgat tgctcgggcc tatcactttg
     2941 ctgaggatgc tcataccgga caaaagcggg cggcgggcga ggcctacagt tcgcattgtc
     3001 tggcaacggc ggtcattctg gctgagttac gcgtcagccc aaccatgatt gcagcgggat
     3061 tattgcacga tgtgccatta gataccaaat ccacttcagc cgatattcgg cgtgagtttg
     3121 gcgacgagat tggactattg gttgatggcg tcagtcgttt aaccaccatt cccaacatca
     3181 cccgagcaga ccagcatttg gagcaaattg aagctgttaa cgccagttta ctgcagcctg
     3241 ccaaaacgga cccaccaaca gatggacgaa gggctgaaca aaacaatgaa gcggcgcgca
     3301 aactcttctt atccatgggg gatgatgttc ggattgtggt tgtaaagctt gcagagcatc
     3361 tgcataatat gcggactctg ggcgcgctcc ccgatgcgtt gcggctgcga atagctcagc
     3421 aaacgctgga tgtgtttgca cctttggcaa atcgattggg catctggcaa atcaaatggg
     3481 aactggaaga cctggctttc cggcacatca acccggacaa gttcaaagag atttcggata
     3541 agctagcaga ccgccgcgaa gaccgcgagc tgcaggtgcg ggcgatcatt gagcagttga
     3601 aaaagattat gggtgatgcc agtatcgagg cggatatctc tggacggccc aaacacattt
     3661 attcgattta tcgaaaaatg gtggataaag gcaagccatt tgaaatggtg cgcgatttac
     3721 gcggcgtgcg attgattgtc aatgatgtgc ctacgtgcta catggcgctt ggcattatcc
     3781 acacacggtg gcgcccgatc ccaaatgaat ttgatgatta cattgcagtg cccaaggaaa
     3841 acaactatca atcgttgcac acggcggtca tctatgatga tgggaaacca ctggaagtgc
     3901 agatccggtc gcgagagatg cacgaaaatg cggagtacgg tatcgcgtca cactggcgct
     3961 acaaggaaaa aggattacgc accgaccaat cctatgagca acgggtgaac tggctgcgca
     4021 gattgctgga atggcgcagc gaactggaag actcgcagga cgcgctggat gggatgaagg
     4081 gcgatgtttt tcagaaccgg gtttatgttt ttactccgcg cggcgatatt attgatttac
     4141 cagccaatgc aacgccgatt gattttgcct accatgtgca tactgaaatt gggcaccggt
     4201 gccgaggtgc gcgcatcaat ggaaagctgg tcgggctgga ctactgtctg aaaacaggcg
     4261 accaggtgga aattttaact gccaagcagg gcgggccaag cagggattgg cttaattcgg
     4321 cgctgggact ggtgcgaacc tcacgggcgc gcgcaaaaat ccgtgcgtgg tttgtgcacc
     4381 aagaccgaga gcacaacctg gctcagggta aggcaatgct ggagcgcgaa ttgaagcggc
     4441 tggcattaac caatgttgac ctggaaaaat tagcccatac attcgactat ccgagcagcg
     4501 aggaactgta tgttgcgctg gggtctggga gtttaccgat tggccgcgtc gtccatgaac
     4561 tgagcgaaga agatcaagag cagcgcgacc agatcgcaac aggaatcacg ggcacgctgc
     4621 caagttcggc ggggttaagt gtaaccgggc taaaaggctt gctgactacg attgcgcgct
     4681 gctgcaaccc ggcgcctggc gatgaggtga ttggatatat tacacaggga cgcggcgcta
     4741 caattcatcg cgtggactgc cccaacctac cccgtttaac cgaaatcagt ccggatcgtt
     4801 tgatcaaagt aagctggggc gaaacgccgc gaacatatcc ggtagacatt gagataaagg
     4861 cctatgacc
//
LOCUS       DAOPVS010000146         4852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_17539_length_4852_cov_41.002918, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000146 DAOPVS010000000
VERSION     DAOPVS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17539_length_4852_cov_41.002918"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKL11_09420"
     CDS             <1..782
                     /locus_tag="PKL11_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNZ13862.1"
                     /translation="DNGWTSTDPVIGLSLDGTGLGTDGAIWGGEVLVGGYRGYERRYH
                     LKYAPLPGGDLSVRKPARMALAHLWAAGLEWAPELPPMQALCYEERTALRGQIERGLN
                     SLPTSSMGRLFDAAAALMGVAQIATYEGQAAIQMEALADPDEVKSYPFDIHGDELDPT
                     PMWEGLLSDWHNGVEQHILAARFHNSVTTVLVECCQKLRAEIGIKTVAFSGGVWQNRY
                     LLERTYRQLREAGFEVLLHRQVPTNDGGLALGQALVAAINI"
     gene            822..1064
                     /locus_tag="PKL11_09425"
     CDS             822..1064
                     /locus_tag="PKL11_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNZ13863.1"
                     /translation="MPGKITEIYEQNGLLMGKIDFGGVLREACLSAIPDAQVGDYTLV
                     HAGFALNRISEEEAQITLETLREIIDLNEELSPEDE"
     gene            1061..2161
                     /gene="hypD"
                     /locus_tag="PKL11_09430"
     CDS             1061..2161
                     /gene="hypD"
                     /locus_tag="PKL11_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNZ13864.1"
                     /translation="MSDIARLTDDFRDGSLVQGVIDEIRRTVTRPWVMMEICGGQTHS
                     ILQSGLDQLLPPEIELVHGPGCPVCVTSLELIDRALAIARQPGVIFCSFGDMLRVPGS
                     QGDLFSIRAAGGQVKVVYSPLDAVRIAQQNPDKQVVFFAIGFETTAPANAASVLQAQA
                     LGLKNYSVLVSQVCVPPAMHAILSSPQRRVQGFLAAGHVCTVMGYWEYKPIAQQYHIP
                     IVVTGFEPLDLVQGILMTVQQLEKGTCEVQNAYPRLVTEEGNRAAQAIIHRVFEPCDR
                     NWRGIGMIPMSGWGLRPELSDFDAEQRFEVAGIQTQESPLCMAGLVLQGLKKPSECPA
                     FGTLCTPQNPLGAPMVSSEGACAAYFRYHRPD"
     gene            2170..>3241
                     /gene="hypE"
                     /locus_tag="PKL11_09435"
     CDS             2170..>3241
                     /gene="hypE"
                     /locus_tag="PKL11_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNZ13865.1"
                     /translation="MTQLPNFEGPVCPMPLQHDETIVIGHGSGGRMTHDLIQKVFLPR
                     LSGGKPGSGNDFAGASLPAQAGLSGRLAVSTDSHIVKPLFFPGGDIGRLAVCGTINDV
                     SMSGATPLYLTAGFILEEGLPMATLEKVLISMQDAAAEAGVQIIAGDTKVVEKGAADG
                     LFINTAGIGWIPAGRFIGGEMARPGDVVLISGNLGDHGIAVLAARGELGFETDVQSDV
                     APLNGLIQTVLEAAPQVHVLRDPTRGGLATTLNEIAHQSNVNITIEEKRIPVQPAVRA
                     ASEMLGFDPLYIANEGKLIVIAPAEQAEAALAAMRAHPYGRNAVRIGEVKAAPERRVL
                     LHTLIGGHRILDMLAGEMLPRIC"
     assembly_gap    3242..3341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3481..4281
                     /locus_tag="PKL11_09440"
     CDS             3481..4281
                     /locus_tag="PKL11_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNZ13866.1"
                     /translation="MKINYHETTNDLQTRIDIHAQYGGRDIDAWMLDLLDLKPGMKIL
                     DVGCGAGKQCLSYHNRLNGQAQILGGDVNPDLLGQAIQRAAEAHAPIQFMPLNFNEPF
                     ALEDNRFDLLSCCFAIYYAEDIPFTIREMHRVLKPGGRLFTTGPMPANKQLFYDIIRE
                     ATGKTIPPMPGSSRFSTGILDAVKATFASAEVHLFENPLTFETAEPFVAYTRASLAED
                     RKLWADFFQGKDDFERIMEQIAKVAERRVKAEGKLVMTKVVGGILAVK"
     gene            4278..>4852
                     /locus_tag="PKL11_09445"
     CDS             4278..>4852
                     /locus_tag="PKL11_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ13867.1"
                     /translation="MTAAGMCFHGQRVCFIGAHPDDIEIGAGALIAQIASKTELLCVT
                     LSDNQKNPALKNLVEEHYRSMDVLGVQREKVVLGQFETRRFPHARQEILEYLIDLNRQ
                     FRPDIVFVHTKADIHQDHGTVTEEALRAFRGTTVLGFDVIRSSYGFFPNFLVEVSAED
                     VEKKIAALSEYRTYDSRYYFNPEITRATLIR"
BASE COUNT         1086 a         1158 c         1472 g         1036 t
ORIGIN      
        1 cagataacgg ctggacaagc acagacccgg tgattgggct cagtctggat ggcaccggac
       61 tgggtacgga tggtgcgatc tggggcggtg aggtgctggt gggaggatac cgtggctacg
      121 agcggcgata tcatctcaag tatgcgccgc tgccaggcgg agacctttcg gtcagaaagc
      181 cggcgcgcat ggcactggcg catctatggg ctgccgggtt ggaatgggcg cccgaactgc
      241 ccccgatgca agcattgtgc tatgaagagc ggacagcgct gcgcggacag atagagcgcg
      301 gactgaacag cctgccgacc tccagcatgg ggcgtttgtt tgatgctgcc gcggcgctga
      361 tgggggtggc gcaaattgct acttacgagg gacaggcggc aattcaaatg gaagcgctgg
      421 ctgatccgga tgaagtcaaa agctatcctt ttgacatcca tggagacgaa ctagatccaa
      481 caccgatgtg ggaaggattg ctgagcgact ggcataacgg tgttgagcaa catattctgg
      541 ccgcccggtt tcacaacagt gtgaccactg tgctggtgga atgctgccag aaattgcggg
      601 cagaaattgg catcaaaaca gttgcattca gcggcggggt gtggcagaat cgctatttgc
      661 tggagcgcac ttaccggcaa ttgcgtgagg ctggttttga agttttgctg caccggcaag
      721 ttccgactaa tgacggcgga ctggcgttgg gacaggcgct ggttgccgca ataaacattt
      781 gatgattatc aataaagtga ggttagatta tgtgtctggg tgtgcctgga aaaattactg
      841 agatttacga acaaaacggc ctgttgatgg gtaaaattga ttttggcggc gtactgcgcg
      901 aagcctgcct gagcgccatc ccagatgcac aggtaggtga ttacactctg gtgcatgccg
      961 gatttgcgtt gaaccgcatc agcgaagaag aggcgcaaat tacgcttgaa acactgcgcg
     1021 agatcattga tttgaacgaa gaattaagcc cggaagacga atgagcgata ttgccagact
     1081 gaccgatgat tttcgtgatg gatcgctggt gcagggggtg attgacgaaa tccggcgcac
     1141 ggtaacgcgc ccgtgggtca tgatggagat ttgtggcggg caaacccatt ccatcctgca
     1201 gtccgggctg gatcaactgc tgccaccgga aattgaactg gtgcacgggc cgggctgccc
     1261 ggtttgcgtc acctcgctgg agctgattga ccgggcgctg gcaattgccc ggcaaccggg
     1321 ggtgatcttc tgctcgtttg gcgatatgct gcgagtgccg ggatcacagg gcgacctgtt
     1381 ctcaattcgg gcggcaggcg ggcaggtgaa agtggtctat tctccgctcg atgcggtgcg
     1441 aattgcacag caaaacccag acaaacaggt ggttttcttt gcgattgggt ttgaaaccac
     1501 cgccccggca aatgcggcga gtgttttaca ggcgcaagcc ctgggactga aaaactactc
     1561 tgtactggta tcacaggtct gtgttccgcc agccatgcat gcaattcttt cctcgccgca
     1621 gcggcgggtg cagggctttc tggcggcagg gcacgtttgc acggtgatgg gatactggga
     1681 atataagcct atcgcgcagc agtaccacat tccgattgtg gttaccggat ttgagccgct
     1741 ggacctggtt cagggtattt tgatgaccgt gcagcagctt gaaaaaggca cctgtgaagt
     1801 tcaaaatgct tacccgcggc tggttaccga agaaggcaac cgggctgccc aggcgatcat
     1861 tcacagggtt tttgagccgt gtgaccgcaa ctggcgcggc attggaatga tccccatgag
     1921 cggctggggg ctgcgtccag agttatccga ttttgacgcc gagcaacgct ttgaggttgc
     1981 ggggattcaa acgcaagagt cgccgctgtg tatggcgggc ctggtgctgc aaggcttgaa
     2041 gaagccatca gaatgcccgg catttgggac actgtgtacc ccgcaaaatc cgctgggggc
     2101 accgatggtc tcgtcagaag gcgcttgtgc cgcttatttc cgctatcatc gcccggattg
     2161 aggagcgcaa tgacccaatt acccaacttt gaaggtccgg tctgcccaat gccattacag
     2221 cacgatgaga cgattgtaat cggacatggc agcggcggac gtatgaccca tgacctgatt
     2281 cagaaagtct ttttaccgcg attgtccggg ggaaagccgg gttctggcaa tgatttcgcc
     2341 ggagccagtt taccagcaca ggcagggctg agtgggcgtc tggcagtttc gacggattcg
     2401 catattgtga aaccattatt cttcccgggc ggagatattg ggcggctggc ggtctgcgga
     2461 actatcaacg acgtgagcat gtccggcgcc accccgctct acctgacagc cgggtttatt
     2521 cttgaagagg ggctgcccat ggcaaccctg gaaaaagtgc tgatttcaat gcaggatgcg
     2581 gcagcagaag ctggtgtaca aatcattgct ggtgatacca aagtggttga aaaaggcgcc
     2641 gcagatggtt tgtttatcaa caccgcgggc attggctgga tacctgccgg gcgattcatc
     2701 gggggggaaa tggcacgccc gggtgatgtg gtgttaatct ccgggaacct gggggaccac
     2761 ggcattgccg tgctggcagc gcggggtgag cttggctttg aaaccgacgt gcagtcggat
     2821 gttgcgccgc tgaatgggtt gattcaaacc gtgctggagg cagcgccgca ggtccatgtt
     2881 ttgagagacc ctacccgcgg cggactggca accacactga acgaaattgc ccaccagagc
     2941 aatgtgaaca ttacaattga ggagaagcgc atcccggttc aacctgccgt gcgggcagcc
     3001 agcgagatgc tgggatttga cccactctac attgccaatg aaggcaaatt gattgtcatt
     3061 gcaccggctg agcaagccga agcagcgctg gcagccatgc gggcgcaccc gtatgggcgg
     3121 aacgcagtgc ggattggaga agtcaaggcg gcgccagaac ggcgggtgct gctgcatacg
     3181 ctgatcggcg ggcaccggat actggatatg ctggcaggtg aaatgctgcc caggatttgc
     3241 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgggcac cggatactgg
     3361 atatgctggc aggtgaaatg ctgcccagga tttgctgacc caacaaaaat tgacataaat
     3421 ctgcatttta gaaatgcgtt ctggtatact tgaaaaaaca aaacaaccaa tgaggttatg
     3481 atgaaaatca attatcacga aacgaccaat gacctgcaaa cccggattga tattcatgct
     3541 caatatggcg ggcgggatat cgatgcctgg atgctggatt tgctggacct gaaacccgga
     3601 atgaaaatcc tggatgtggg ctgcggcgcg ggcaaacaat gcctgtctta ccacaaccga
     3661 ctgaatggac aggcacagat tctgggcggc gatgtaaacc cggatttgct ggggcaagcc
     3721 attcaacgag cagcagaggc gcacgcgccg atccaattta tgccattgaa tttcaacgag
     3781 ccgtttgcgc tggaagacaa ccgctttgac ctactgtctt gctgctttgc aatttactat
     3841 gcggaagata ttccgttcac aattcgcgag atgcaccggg tgctcaagcc cggcggacgg
     3901 ctgttcacta cggggccgat gcctgcaaac aagcagctat tctatgacat catccgggaa
     3961 gcgaccggga agaccatccc accgatgccg ggcagttccc gcttcagcac cgggattctg
     4021 gatgcggtca aggcaacctt cgccagtgcg gaagttcacc tgtttgaaaa tccgttgaca
     4081 tttgaaaccg cagagccttt tgtggcttat acccgcgcct cgctggcaga agaccgcaaa
     4141 ttgtgggcgg atttcttcca gggcaaggac gattttgagc gcattatgga gcagattgcc
     4201 aaagttgccg agcgccgcgt gaaggctgaa ggcaaactgg tgatgaccaa ggtggttggc
     4261 ggcattctgg cggtcaaatg acagccgctg gcatgtgctt tcacgggcag cgggtatgct
     4321 ttattggcgc tcacccggat gatattgaaa ttggcgccgg ggcattaatt gctcagattg
     4381 cgtccaagac ggaattgctg tgtgtgaccc tgtcggataa tcagaaaaat ccggcgctga
     4441 aaaacctggt ggaagagcat tatcgcagta tggatgtgct gggggtgcag cgcgagaagg
     4501 tggtgctggg gcagtttgag acccggcgat ttccgcacgc ccgccaggag attctggaat
     4561 atttgattga cctcaaccgg cagttccgcc cagatatcgt gtttgtgcat accaaagcgg
     4621 atattcatca agatcatggc acggtaaccg aggaggcgct gcgagctttt cgaggcacga
     4681 ccgttctggg gtttgatgta attcgcagca gctatgggtt ctttcccaat tttctggttg
     4741 aagtgagcgc agaggatgtg gaaaagaaaa ttgccgcgct gagcgagtat cgcacctacg
     4801 acagccgata ttacttcaat ccagagatta cccgggccac actgatccgg ca
//
LOCUS       DAOPVS010000147         4843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_17577_length_4843_cov_56.644528, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000147 DAOPVS010000000
VERSION     DAOPVS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17577_length_4843_cov_56.644528"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..>1668
                     /gene="glyA"
                     /locus_tag="PKL11_09450"
     CDS             359..>1668
                     /gene="glyA"
                     /locus_tag="PKL11_09450"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNZ13868.1"
                     /translation="MADFLVRGTLADVDPDVYELCQIEAERQVRKLILIPSESQAPVA
                     VREALGSVFQNLYAEGYPDEETRFMPEEEILDYTARLSHYRRYADPRYYKGVEYADVI
                     ESLARRRCAEAFATPKFSADQIFVNVQALSGAPANNAVYGALIAPGDTILGMNLLHGG
                     HLSHGSSVNRSGKLYKVVHYNVDPTTEQIDYDAVEALARTHMPKVIIGGYSSYPWSPD
                     WERFRAIADAVGAILLADVSHIAGLIAGGVLPSPIGYAHVITFTTHKTLCGPRGACIL
                     TTDGALARKIDRSVFPGEQGGPHIHAVAALATTFKLAQTPEFKALQAQIIKNCQALTN
                     RLRERGLRIPFGGSDTHLGNIDCKTIVGPDGTTLSGDMAARILDVAGIVCNRNTIPGD
                     KTALFSTGIRYGTPWMTQRGLKEAEMVQVADAIADILYACKPYT"
     assembly_gap    1669..1768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1769..3181
                     /gene="gcvT"
                     /locus_tag="PKL11_09455"
     CDS             <1769..3181
                     /gene="gcvT"
                     /locus_tag="PKL11_09455"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNZ13869.1"
                     /translation="GYVSFDEDLSRRLPGPIYVTDTAKPITAARGNAAAPKKPYSIGM
                     SAGKGKALPHFKWKEPESAEIRRTPIYEIHKQMGAKMIPFAGWEMPVWYTSVTEEHLA
                     CRQAAGLFDVAHMGVYQAEGPDAVAFLDSVCGNDISALAVGESCYTHFLDPDGNVIDD
                     TLVYRRDADRYLVVVNASNDDKDWAWLNAVRDGKVLVDNEHPWVRAFGRNVVFRNLRD
                     PKAGADMRIDIALQGPKSRDILLSLGVDAPTRRRIMALKRTQLCEAVVGGFELVVSRT
                     GYTGEKMAFELFVHPDKAADLWNALRKAGEPLGMRACGLGARDSLRTEAGLPLYGHEM
                     GGDLGLGVAEAGFGSYVKTYKPWFIGRTAFINREKARQGVVVRFRFTEKGVRMAHLHD
                     PVLDRKGRVIGVVTSCAIDAEGFLTGQAFIETKAAEEGAPIAIFQSAPKAAGKAPAEL
                     ATGDRVTMPTPAVVISRFPK"
     gene            3226..4443
                     /locus_tag="PKL11_09460"
     CDS             3226..4443
                     /locus_tag="PKL11_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNZ13870.1"
                     /translation="MHTQWEHRYAQRTQRMKGSAIRELLKLTELPGVISFAGGLPAPD
                     VFPIDEFKRACMTVLEKQGPQSLQYGSSEGYQPLREMIARHTSRQGIGVTADNVMITS
                     GSQQALDLLGKVFINHGDRIIVEAPTYLGALQAWNAYGAEYITVESDEYGIVPESLAE
                     ALRTSPKFMYVLPNFQNPTGITTSLERRKTLIEMADRYGVPIIEDDPYGQLRYEGEDI
                     PSVCVLDNQIRAQNGVYTGNVIYLSTFSKILAPGLRLAWVIAPADVIRKLVLAKQGAD
                     LHTATFNQFVAYEVGHGGFIDQHVLTIRKVYSERRDVMLDALEEHMPKGVKWTHPLGG
                     LFLWITMPKGMDSSEVFKIAVEKEKVAFVPGYSFFPNGGGENTMRLNFSYSNPETINE
                     GIARLGRVLKSIV"
     gene            4617..>4843
                     /locus_tag="PKL11_09465"
     CDS             4617..>4843
                     /locus_tag="PKL11_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ13871.1"
                     /translation="MPRPTFFNLPDEKRQKILEAAIDEFAERDYESASVSKIVIQAGI
                     PKGSLYQYFVDKSDLHHYLLELALQKKAEMMA"
BASE COUNT         1004 a         1248 c         1491 g         1000 t
ORIGIN      
        1 agggtcgcgc cggttgttat ggggtgtagg ggcggaccta tgtgcatgcg gcccggatgg
       61 gctgtccgcc cggtgtagca ttaaaaatac tcagggcgca caacctgttc aggttgcttg
      121 cacacaggtg cgcccctaca ggaaaatcag gccggcatta tcgactccgt ttggatgagg
      181 ctgcttttac ggacttgatt ttggtccggc ggggccgccg ggttgtgaca atcaacgggt
      241 tgtgggccaa tttcaggaat ctgcaacata caatctgtga cctctttcac ttgaccagtc
      301 aaattggcaa gactacaata atggtaactg caggttaaat ttaataagga gcgattcaat
      361 ggccgatttc cttgtgcgtg ggactctggc ggatgttgac cccgatgtgt acgagctgtg
      421 tcagatcgag gctgaacgtc aggtccgcaa attgattctg attccgagtg agagccaggc
      481 gccagtggct gtccgtgagg cgcttggttc tgttttccag aatctgtatg ctgaaggtta
      541 cccggacgaa gaaacccggt tcatgcctga ggaagagatt ctggattaca ctgcccgcct
      601 gtcgcattac cggcgctatg ccgatccgcg ctattataag ggcgtggagt atgctgatgt
      661 gattgaaagc ctggcacgcc ggcgctgcgc cgaagccttt gccacgccca agttctcggc
      721 ggaccagatt tttgtaaatg tgcaggcgct ttcgggcgcc cctgccaata atgcggttta
      781 tggggcgctg attgcccccg gcgacaccat tctgggtatg aacctgctgc atgggggtca
      841 cctgtcgcac ggatcatcag tgaaccgctc tggcaaactg tacaaggtgg tgcattacaa
      901 tgtggacccg accaccgaac agattgacta tgatgctgtg gaagcgctgg cgcgcaccca
      961 tatgcccaag gtcatcatcg gtggttattc atcttacccc tggtcgccgg actgggaacg
     1021 gttccgcgcg attgcggacg cggtgggcgc gattttgctg gcggatgttt cgcacattgc
     1081 gggcttgatt gccggcggtg tgctgccatc gcccattggt tatgcgcatg tgattacctt
     1141 caccacgcat aagaccctct gcgggccgcg cggcgcgtgc attttgacca ccgatggggc
     1201 gctggcgcgc aagattgacc gctcggtgtt cccgggtgag cagggcgggc cgcatattca
     1261 tgcggtggcg gcgctggcga ccaccttcaa gctggcgcaa acgccggaat tcaaggcgct
     1321 gcaggcgcag atcatcaaga actgccaggc gctgaccaac cggctgcgcg aacgcgggct
     1381 gcggatcccg tttggcgggt cggatacgca cctgggcaat attgactgca agacaattgt
     1441 ggggccggac ggcaccacgc tctccggtga tatggcggcg cgcattctgg atgtggccgg
     1501 gattgtgtgc aaccgcaata ccatccccgg cgacaagacg gcgctgttct ccacgggcat
     1561 ccgctatggc acgccctgga tgacgcagcg cggcctgaaa gaagccgaga tggtgcaggt
     1621 ggccgacgcg attgccgaca ttctgtatgc ctgcaagcct tacaccatnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gtatgtgagc tttgatgaag atctgagccg
     1801 ccgcctgccg gggccgattt atgtgacgga cacggccaag cccatcactg cggcgcgcgg
     1861 gaatgcggct gcgccgaaga aaccctattc tattgggatg agcgccggca agggcaaggc
     1921 cctgccccac ttcaagtgga aggaacccga atcggctgaa attcggcgca caccgattta
     1981 tgaaattcac aagcagatgg gcgccaagat gattccgttt gcgggctggg agatgccggt
     2041 gtggtacacc tcggtgaccg aggaacacct ggcctgccgc caggcggcgg ggctctttga
     2101 tgtggcacac atgggcgtgt atcaggcaga gggaccggat gcggtggcct tcctggacag
     2161 cgtctgcggc aatgatatca gcgcgctggc ggtgggagaa tcttgctata cgcacttcct
     2221 tgatcctgat gggaacgtga ttgatgatac gctggtttac cggcgcgatg ctgaccgcta
     2281 cctggtggtg gtgaatgcat ctaatgacga caaggattgg gcctggttga atgcggtgcg
     2341 cgatggcaag gtgctggtgg ataacgagca cccctgggtg cgtgcctttg gccgcaatgt
     2401 ggtcttccgc aacctgcgcg acccgaaagc gggcgccgat atgcgaattg atattgcgct
     2461 gcagggaccg aaatcccgcg acatcctgct ctcactaggc gtggatgcgc ccacgcgccg
     2521 gcggatcatg gcactgaaac gcacacaact gtgcgaagca gtggtcggcg ggttcgaact
     2581 ggtagtctca cgcacgggtt acaccggcga gaagatggcg tttgaactgt ttgtgcaccc
     2641 cgacaaagcg gctgacctgt ggaacgcact gcgcaaagcg ggcgaaccgc tgggcatgcg
     2701 agcctgcggc ctgggggcac gcgactcact gcgcaccgaa gccgggctgc cgctctacgg
     2761 acatgaaatg ggcggcgacc tgggtttggg tgtggcagaa gccgggtttg gctcttacgt
     2821 gaagacttac aaaccctggt tcattgggcg gacggcgttc atcaaccgcg aaaaggcccg
     2881 ccagggcgtg gtggtgcggt tccgcttcac cgaaaagggc gtgcgcatgg cccacctaca
     2941 tgatccggtg ctggatcgga agggccgcgt gattggcgtg gtgaccagct gcgcgattga
     3001 cgcggaaggc ttcctgaccg ggcaggcgtt cattgagacg aaggccgccg aagaaggcgc
     3061 cccgattgcg atcttccaga gcgctcccaa agctgcgggc aaggctccgg cagaattagc
     3121 caccggtgac cgcgtgacca tgccgacgcc ggcagtggtc atcagccgct tccccaaata
     3181 gaccgagagt catcccggta aaccaattct acacaaggag aaagaatgca tactcaatgg
     3241 gaacatcgct acgcacagcg cacacagcga atgaagggat cagcaatccg tgagctgctc
     3301 aagctgacgg agctacccgg ggtcatctcg tttgcgggcg ggctgcctgc gccagatgtc
     3361 tttccaattg acgagttcaa acgggcctgt atgacggtgc tggaaaaaca gggcccacag
     3421 tcactgcagt atggatcgtc tgagggttat cagccgctgc gggagatgat tgcccgccat
     3481 accagccgcc aggggattgg cgtgacggcg gataatgtga tgattacatc ggggtcacag
     3541 caagcgttgg acctgctggg taaggtcttt atcaatcatg gcgaccgcat cattgttgaa
     3601 gcccccacct atctgggtgc gctgcaggcc tggaatgcct atggggccga gtacattacg
     3661 gtcgagtcgg atgaatatgg gattgtgccc gagtcgctgg ctgaagcgct gcgcaccagc
     3721 ccgaagttta tgtatgtgct gcccaacttc cagaacccga cgggcatcac cacctcactg
     3781 gaacggcgca agaccctgat tgaaatggct gaccgctacg gtgtgccaat cattgaggac
     3841 gacccctacg ggcaactgcg ctacgaaggc gaggatatcc cctcggtttg tgtgctggac
     3901 aaccagatac gcgcccagaa cggcgtgtac acgggcaatg tgatctacct gagcacgttc
     3961 tccaagattc tggcgccggg cctgcggttg gcctgggtga ttgcaccggc agacgtgatt
     4021 cgcaagctgg tgctggcaaa gcagggcgct gacctgcaca ccgccacgtt taatcagttt
     4081 gtggcgtatg aggttgggca cggcgggttc attgaccagc atgtgctgac catccgtaag
     4141 gtgtacagcg aacgccggga tgtgatgctg gatgcgttgg aagagcatat gcccaagggc
     4201 gtcaagtgga cgcatccgct gggcggtctg ttcctgtgga ttacgatgcc taagggaatg
     4261 gactcttcgg aagtctttaa aattgcggtg gaaaaagaga aggtggcttt tgtgccaggc
     4321 tactcgttct tcccgaacgg cggcggcgag aataccatgc ggttgaattt ctcgtacagc
     4381 aatccagaga cgatcaacga aggcattgcc cggctggggc gtgtgctgaa gtcaatcgta
     4441 taactggggt agatcaagta agttttacct cccggtgagc caatagctcc ccgggagttt
     4501 tatttttcgg ctcattatga gccatctatc gcttgacata cggaggaaaa gttatataat
     4561 acgactggtc agtcatatga ccgagcggtc ataaaatcaa aacctgagga acgcgattgc
     4621 cacgccctac ttttttcaac ctgccggatg aaaaacggca aaagatactc gaagcggcca
     4681 ttgatgaatt tgcagagcgc gattatgaga gcgcgtcagt ctccaaaatt gtgattcagg
     4741 ctgggattcc caaaggcagt ctttatcagt attttgtgga taagagcgac ctgcatcatt
     4801 atttgttaga gctggcgtta cagaagaagg ctgagatgat ggc
//
LOCUS       DAOPVS010000148         4842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_17580_length_4842_cov_61.005431, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000148 DAOPVS010000000
VERSION     DAOPVS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17580_length_4842_cov_61.005431"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="surE"
                     /locus_tag="PKL11_09470"
     CDS             complement(<1..698)
                     /gene="surE"
                     /locus_tag="PKL11_09470"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNZ13872.1"
                     /translation="MHILVTNDDGITAPGLLALAQAMQPLGKVSVFAPDHNWSASGHV
                     KTLHRPLRVKETRLADGTPALTTDGAPSDCVALAVLGIVEPLDLVVSGINPNANLGHD
                     VTYSGTVTAAMEAAIWGIPGLAFSVETPEDHQGAVDYSTTAAVARRVAEQVIAQPHPR
                     DLLLNVNVPYLPESELKGYRITRMGLRIYRDALVRREDPRGRPYYWIGGDAPTGVPDE
                     GTDIGALANGYVSIT"
     gene            complement(704..1756)
                     /gene="add"
                     /locus_tag="PKL11_09475"
     CDS             complement(704..1756)
                     /gene="add"
                     /locus_tag="PKL11_09475"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNZ13873.1"
                     /translation="MPEAHPVQSDTLFQQMPKVELHRHLEGSLRTETLLDIARLHGIT
                     VPLRPGLRALVQMQSGDPLTFTNFLSKFQTLRMFYRSPEVIARITREAVEDAAADQVR
                     YMELRFTPVALSRAEGFPLAQVIDWVADAARQAEAEFGVITRLIVSINRHEPVNVAEE
                     AVDLAIQRRAKGIVGIDLAGNEANFAATPFLGLLRGARRSGMFLTVHAGEWNGPENVR
                     EAIEDLNADRIGHGIRVMEQESVSALASEHGTAFEVCVTSNYQTGAVPSLSEHPLGRM
                     LMAGINVTLNTDDPSISQITLSNEYQVAHRDLGLPLSEIKARVLAAAQAAFLPPEEKQ
                     ALVERLKTELQSLPLE"
     gene            complement(1808..2623)
                     /locus_tag="PKL11_09480"
     CDS             complement(1808..2623)
                     /locus_tag="PKL11_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNZ13874.1"
                     /translation="MSISKRLEEARQAYTAGDAAASAQAHSAAKIAAAAREEHGPASS
                     QYIGNLVYGGLDGIITTFAVVSGVAGAQLASGIILILGLANLFGDGFSMAIGAYLSSK
                     SEKEYYNRERQREAWEVEHFPEGEKQELLELYRAQGYPDEDAQKLVEIKTRDKDRWLD
                     TMMVEELGLMHDDRNPRTEAWMTFAAFVVAGALPLLAYVADWIFPFTLAPQTAFFISL
                     GLSALALFGLGAAKVLVTQRNALRSGLEMLLVGGLAAGVAYGIGALLKGLGGG"
     gene            complement(2637..4361)
                     /locus_tag="PKL11_09485"
     CDS             complement(2637..4361)
                     /locus_tag="PKL11_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13875.1"
                     /translation="MMRWIRLAGIIGGLLMAGLAVAADYLGIGLTTGSDAGFGRAQTL
                     LLYAGLLVLSGALILPGNTGQASRRGWLPGIILLGVSILIDLVLLYYLRFVDEGDTMT
                     TGWLMSQGYVLYRDLFSHHAPLPYAWAALVTLIAGRSFMALRLSVILFKVGLIGLGMR
                     LSRQYTLLGIVALVWSAIGHFYFGHMLLYDLFAGVPLLIMLVIVLAAGSQKITLGDGH
                     FWVLGGLAATTLFSDPTKIYAVAPLMLWALAFAARQNGWRAGAKALLQLAAIPVLALG
                     LMVGLLGIQGALPAAWADLYQFNTGIYARYAEYQATDPVNYLHQVISGLEISSPQWQP
                     LTFTLEPSTAVDGWLFTGFFYRLMGLLSVILLILRRRFGLAAAVYLALAGMLTGRNTT
                     FFHAQSFVLAALALAAGLAVEQDQNRGWAISWGQRVAGTLAAAGLIFLTVMGIWFIAS
                     HPERLPYARRYADYEARAAALKDLACGQPEARLAYYPVDPLVYFFAGMEPPGRFTYLQ
                     PWVAELGQAETIDALRTTPTVIYIETDANIWGYVAEDYLAPMITFLNENYRSAGETVW
                     VSPLIECP"
     gene            complement(4498..>4842)
                     /locus_tag="PKL11_09490"
     CDS             complement(4498..>4842)
                     /locus_tag="PKL11_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13876.1"
                     /translation="VKINDILGLNQLPTTAVINPGQTLVIYPSSTPSPSPTITETPVP
                     ATKTPTRTPTPRTPTATRTVTPSPTPTVRSLLEDIKLEGDGGARQMIGYGLIGICGIG
                     LAALLISSLRKK"
BASE COUNT         1068 a         1534 c         1280 g          960 t
ORIGIN      
        1 gtaatcgaga catacccgtt tgccagcgcc ccaatatcgg tgccctcatc cggcacgccg
       61 gtcggggcgt cgccgccgat ccagtaatac ggccgcccgc gcgggtcttc ccgccgcacc
      121 agcgcgtctc ggtaaatccg cagtcccatc cgggtgatgc ggtaaccttt taattctgat
      181 tcgggcaggt acggcacatt cacgttgagc agcaaatcgc gcgggtgggg ttgggcaatc
      241 acttgctcgg ctacccggcg cgccactgct gctgtggtgc tgtaatccac cgcgccctgg
      301 tgatcttccg gcgtttccac cgagaacgcc agccctggaa tgccccagat ggcggcttcc
      361 attgccgcag tgactgtgcc agaataggtc acatcatgcc ccaggttggc attcgggttg
      421 atgcccgata ccaccagatc cagcggctca acaatcccca gcaccgccag cgccacacaa
      481 tcggagggcg ctccgtccgt ggtcagggca ggtgtgccat cagccagccg ggtttctttc
      541 acccgcagcg ggcggtgcag cgttttgaca tgtcccgaag ccgaccagtt atggtcgggc
      601 gcaaatacgc tcactttgcc aagcggctgc atggcctgtg ccagcgccag cagacccggc
      661 gcggtgatgc cgtcatcatt tgtcaccagt atatgcattt gagttattcc aacggtaaag
      721 attgaagctc ggttttcaga cgctccacca acgcctgttt ttcttcaggc ggcaggaagg
      781 cagcctgggc ggctgccaga acgcgagctt tgatttctga taagggcagc cccagatcgc
      841 ggtgggccac ctgatactca tttgagagtg taatttgaga aatactcggg tcatcagtgt
      901 tcaacgttac attgatgccc gccatcaaca tgcgccccag cgggtgctcg ctcaggctgg
      961 gcaccgcccc ggtctgatag ttgctggtca cacagacttc gaacgctgtg ccgtgttcgc
     1021 tggccagcgc actgaccgat tcttgttcca tcacccgaat cccgtgccca attcggtcag
     1081 cgttcaaatc ttcaatcgcc tcgcgcacat tttctggacc attccactcg ccggcatgaa
     1141 ccgtcaggaa catcccgctg cgcctggctc cccgcagcaa acccaggaag ggtgtggccg
     1201 caaaatttgc ttcgttgccg gccaggtcaa tccccacaat cccttttgcc cgccgctgaa
     1261 tcgccaggtc aactgcttct tccgccacgt tgacgggctc gtgccggttg atgctcacaa
     1321 tcaggcgggt aatgacccca aattcagctt cggcctgacg ggctgcatca gccacccagt
     1381 caatcacctg ggctaacgga aaaccttctg cccggctcaa ggccactggc gtgaagcgca
     1441 gctccatata ccgcacctga tccgccgccg catcttccac tgcctcgcgg gtgattcgcg
     1501 caatcacttc cggcgagcgg tagaacatgc gcagtgtctg aaattttgac agaaaattgg
     1561 tgaaagtcag cgggtcgcca gactgcatct gcaccagggc gcgcagcccc gggcgcagtg
     1621 gaacagtaat cccgtgcagc cgggcgatat ccagcaacgt ttcagtccgc agcgagcctt
     1681 ccaggtgacg atgtaattcc accttgggca tctgttggaa gagtgtgtca gactgaactg
     1741 gatgagcttc cggcattcaa cctcgaattt tgcttattat agcttaatcc cataaatcaa
     1801 gggtgaatta tccacccccc aaacctttaa gcagcgcacc gatcccataa gccacacccg
     1861 cggccagccc acccaccagc agcatttcca gaccactgcg gagcgcattg cgctgtgtca
     1921 ccagcacttt ggctgcaccc aaaccaaaca gagccaacgc cgatagtccc agggagatga
     1981 aaaatgctgt ttggggtgcc agtgtaaacg ggaaaatcca atcggccaca taggccagca
     2041 gcggcagagc gccggcaacc acaaatgccg caaaggtcat ccaggcttcg gtgcgcgggt
     2101 tgcggtcatc gtgcatcaac cccagttctt ccaccatcat ggtatccagc caacgatcct
     2161 tatcgcgcgt cttaatctcc accagctttt gagcatcttc atcaggatag ccctgagccc
     2221 ggtaaagctc caacagttcc tgcttttcac cttcagggaa atgttcaacc tcccaggctt
     2281 cccgctgccg ctcccggttg taatattcct tctcgctttt cgaggagagg taggcgccaa
     2341 tcgccattga aaacccgtcg ccaaacaggt tggccaatcc cagaatcaaa ataataccag
     2401 atgccagctg cgctccggcc accccactga ccacggcaaa cgtggtgata attccgtcca
     2461 acccgccata gaccaggttg ccgatatatt gtgaactggc cgggccatgc tcttcgcggg
     2521 cggcggctgc aatttttgcg gcgctgtggg cctgcgccga ggcagcggca tcgccagccg
     2581 tataagcctg gcgggcttcc tccaaacgtt ttgagatgct catctttgct ccttgtttat
     2641 ggacattcga tcagcggcga aacccacaca gtttcacctg cagaccgata attttcattc
     2701 aaaaacgtta tcatcggcgc cagataatct tcagccacat agccccagat attggcatcc
     2761 gtctcaatat agatcaccgt gggggtggtg cgcagcgcgt caatggtttc agcctgtccc
     2821 aactcagcca cccagggctg caggtaagtg aagcgtcccg gtggttccat gccggcaaag
     2881 aaatacacca gcgggtccac cgggtagtag gctaatcggg cctctggttg gccgcaggcc
     2941 aggtctttca acgccgccgc ccgcgcctca taatcagcat aacgccgggc ataaggcagc
     3001 cgctccgggt ggctggcaat gaaccagatt cccatcacag tcagaaatat caatccggca
     3061 gcggccaggg tgccagccac gcgctgcccc catgatatcg cccaccctcg gttttgatct
     3121 tgctcaacag ccagcccggc agccagtgcc agcgctgcca gcacaaaact ctgagcatgg
     3181 aaaaaagtcg tgttgcgccc ggtcaacatg cccgccagcg ccaggtaaac agccgcggcc
     3241 agtccgaacc gcctgcgcag aatgagcagg atcacactca acagccccat cagccggtaa
     3301 aagaatccgg tgaacagcca cccatccacc gccgtcgaag gttccagtgt aaacgtcagc
     3361 ggctgccact gaggcgagct tatttccagc ccgctgataa cctgatgcag gtaattaacc
     3421 gggtcagtgg cctgatattc agcataacgg gcataaatcc cggtgttgaa ctgatataga
     3481 tctgcccagg ctgccggcag tgccccctgg atacccaata gtcccaccat caaccccaac
     3541 gccagcaccg gaattgcagc caattgcagc agcgcctttg cgccagcgcg ccagccattc
     3601 tgccgggcgg caaacgccag cgcccagagc atcaacggcg ccacggcata aatcttggtc
     3661 gggtcggaaa acagcgtcgt tgcagccagt cctcccaaca cccaaaaatg tccatccccc
     3721 agggtgattt tttgacttcc ggctgccagt acaatcacca gcattatgag cagcggaacc
     3781 ccggcaaata gatcatacag cagcatatga ccaaaataaa aatgcccgat ggcgctccag
     3841 accagcgcta caattcccag cagcgtatat tggcgcgaca gccgcatgcc cagcccaatg
     3901 agcccaacct tgaataaaat caccgacagc cgcagtgcca taaaagagcg ccccgctatc
     3961 agcgtcacca gcgccgccca ggcgtagggc agcggcgcgt gatgagagaa caggtcacgg
     4021 tacaagacat agccctggct catcaaccag cccgtggtca tggtatcgcc ctcatccaca
     4081 aaccgcaggt aatacagtaa aaccaagtct atgaggatgg acacccccag caaaatgatc
     4141 cccggcagcc agccgcgcct ggacgcttgg ccagtgtttc ccggcagaat caacgcgccg
     4201 cttaaaacca gcagcccggc atacagcagc agggtttgag cgcggccaaa accagcatca
     4261 ctgcctgtgg ttaatccgat tcccaggtaa tctgccgcca cagccaggcc ggccatcagc
     4321 agcccgccga tgatcccggc caggcgaatc cagcgcatca cgtttggctt cataagccgt
     4381 tattataatc ccgctggagg tgttggggcg cttgctggcg ctgtaactca atgcaaaaac
     4441 aaacccgccg ggtaaacatt cggcgggttt tatggacttc agtttggcag cttttaatta
     4501 tttcttccgc aggctgctaa tcagcaaggc cgccagccca atgccacaaa tgccaatcag
     4561 cccataccca atcatctggc gcgcaccgcc atcgccctcc agtttgatgt cttccagcag
     4621 ggatcgaacc gttggggtgg ggctgggggt gacggtgcgt gtagccgtgg gggtgcgcgg
     4681 cgtcggcgtg cgcgtgggtg ttttggtggc gggcaccggc gtttcggtaa tcgttggcga
     4741 tggactgggg gtggaggatg ggtagatcac cagcgtctgc cccgggttga tgaccgccgt
     4801 agtcggcagt tgattcaacc ctagaatgtc gttgatcttg ac
//
LOCUS       DAOPVS010000149         4784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_17889_length_4784_cov_44.182491, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000149 DAOPVS010000000
VERSION     DAOPVS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17889_length_4784_cov_44.182491"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3676)
                     /locus_tag="PKL11_09495"
     CDS             complement(<1..3676)
                     /locus_tag="PKL11_09495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13877.1"
                     /translation="MSLTLPAARLNLIALSAEELRLYRDDPEALRLALGLARLHADMS
                     EPLQRAVRIKLERMALLPVEQHPWQTYWLIVLTETQFGAGLIGFKGVPDAEGRAEVGY
                     GIAPEVEGQGYMTEALRRLAEWAWTRPEIRGITATVDPENIASQRVLEKSGFLHLGRA
                     PDEPNLLAYELRRPQTSPLGTAPLPAGVRPGRISGERRQVTVLFADLSGFTTLSEQLD
                     AEEVCGLLNASFDVLVPVIEKYGGVVDKFIGDEVMAVFGAPVAHEDDPARALRAALEM
                     MTAMAAFNAVHNTTLGLHVGINTGLVIAGGIGSQGQQHDTVIGSAVNLAARLKDISER
                     STIMLGPQTYRLTAPLFEFASLPPVLVKGLTEPVQAYALLGLKDRPGSVRGLAGMDSP
                     MVGRVAELAALLQLSAAVQGGRGGAALVLGEPGLGKSRLIAEWRAALSGQTLRWGLGR
                     CLSYSQQQAYSLIQDLLRSLLGTAPGASPIEVAAALHTWLPAAESEAAYLRHLLGLPL
                     DDARLAQIHTLDAQTLQTYTLNALRRLLLALAERTPLHLICEDIHWADASSVDLLTRL
                     LPLLNEAPVLLCAVSRAEPDSPGWRLVTAIRQSADLPNCHDLALNPLSPGDGRNLLKN
                     IFHHSALPEALYDRILDRAGGNPFFVEEVVRMWVDQGVLVRGSGGWHTTHLTESVEIP
                     DTLHALLLARMDRLPEPARRTLQVAAVLGRRFSARLLQEITGQHTAGLGLHLNTLRAA
                     EIIVPAEVGTEDEYTFRHNLVQEAAYQSLLRDDRRDLHRAAAEALERLYPDLTIDLAH
                     LLAHHYHEAGDTSRSLRYFVLAGDADFKQYAVAEAIGHYTHALEHARELGPDLPTSNW
                     QHLGLQLGRALELDARYAEALAVYDELEALARQRAEEPLELAVLLESARLYCTPNPAF
                     NPGRGAEYAGRALALAQRLGDRAAEARVHWVLSLLYWLINQPDTALENGEISLKLARE
                     LNLREQLAFTLHDLTRPFMLVHRQREAMTGLDESRRLWRELNNLPMLADNLNTTADIY
                     GQLRQPERELEYAAEAYELSRTIGNLWNQAFSRGLMGEAQQMLGHYPESILALRESLE
                     VSKQAGLGILARRVLFELGWLLACLGDTDAARRVLFENQDQFGPQEGYVPFMVPSLGW
                     LLLADIEILAGQVRVAEDDLRQAEALMPAETGGRVSAALNSTRAQLAVIHNDEPAARQ
                     AVAALPEAIAQAHASSVDRTW"
     gene            complement(3673..4680)
                     /locus_tag="PKL11_09500"
     CDS             complement(3673..4680)
                     /locus_tag="PKL11_09500"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ13878.1"
                     /translation="MNVLVTGSTGFIGAALCRALVEQGHQVRAFHRASSNLRLLEDLP
                     VEHLVGDLTQPDTLAPALAGMEVVFHAAAMLGGKHDEPGRMYAVTVEGTRALLSEARR
                     AGVRRVVHTSSVAALGVPPLPARNAAPLLMDETHTWNFRPDYWPYGYSKYLAEMEVQR
                     AVSEGLDVVTVNPAMVVGAGDIYRKSSSLVVRVAQRRLPMLTEGGLNIVHIEDVVAGH
                     LAALQRGQSGQRYILGGHNLSHQAYLQQIAAVVGAPAPTLVLPAGLLRRLALPIRLMS
                     PFLDLPFSPVTFRKAGYYFYVDTRKSRTALGLPPPRPVSEAIRSAYDWFIQTGVLEAP
                     R"
BASE COUNT          903 a         1508 c         1606 g          767 t
ORIGIN      
        1 gccaggtgcg atccacactg gaggcgtggg cctgggcaat ggcctcgggc agggcagcca
       61 ccgcctgacg ggcagcgggt tcgtcgttat ggatcaccgc caactgcgcc cgggtgctgt
      121 tcagggcagc agacacccgc cctcccgtct cagcgggcat caaagcctcc gcctggcgca
      181 gatcgtcctc ggcaacgcgc acctgcccgg ccagaatttc aatatctgcc aggagcagcc
      241 agcccagcga gggcaccata aagggcacat agccttcctg cgggccaaac tggtcttgat
      301 tttcaaacag gacgcggcgg gcggcatcgg tatcgcccaa acacgccagc agccagccca
      361 actcaaagag cacgcggcgg gccaaaatgc ccagcccggc ctgtttgctc acttccagtg
      421 attcgcgcag ggctaaaatg gattccggat agtgccccag catctgctgc gcctcgccca
      481 tcaggccgcg gctgaaggcc tgattccaca ggttgccgat agtgcggctc aattcgtaag
      541 cctcggcggc gtattccagc tcgcgctcgg gctggcgaag ctgcccgtaa atatcggcgg
      601 tggtgttcag gttatcggcc agcatgggca ggttattcag ttcacgccac agccggcggg
      661 attcgtcgag gccagtcatg gcctcgcgct ggcggtgcac cagcataaag gggcgggtga
      721 ggtcatgcag ggtgaaggcc aactgctcgc gcagattgag ctcgcgggcc agcttgagcg
      781 aaatttcgcc gttttcaagc gcggtatcgg gctggttgat cagccagtag agcagcgaca
      841 aaacccagtg cacccgcgcc tcggcagccc ggtcacccag gcgctgggcc agggccaggg
      901 cgcgcccggc atattcagcg ccgcggccgg ggttgaaggc cgggttgggg gtgcagtaga
      961 gtcgggccga ttccagcagg accgccagtt ccagcggttc ttcagcgcgc tggcgggcca
     1021 gggcttccag ttcatcataa acagccagcg cctccgcgta gcgggcatcc aactccaggg
     1081 cgcggccaag ctgcaagccc aggtgctgcc agttgctggt gggcaggtcg gggcccagct
     1141 cgcgggcgtg ctccagggcg tgggtgtaat ggccaatcgc ctcggccacg gcatactgct
     1201 tgaagtcggc atcgccagcc agcacaaaat aacgcagcga gcggctggta tcgccagcct
     1261 catgataatg gtgcgccagc aggtgcgcca gatcaatcgt cagatcgggg tagagccgtt
     1321 ccagcgcctc ggcggcggcg cggtgcagat cgcggcggtc atcgcgcagc agggattggt
     1381 aggcggcctc ttgcaccagg ttgtgccgga aggtgtattc atcctcagtg ccaacctccg
     1441 cggggacgat gatttcggcg gcacgcaggg tgttgaggtg gaggcccaac ccggcggtat
     1501 gctgcccggt gatttcttgc agcaggcggg cagagaagcg ccgtcccagc acggccgcca
     1561 cctgcaaggt gcggcgggca ggctcgggca ggcgatccat gcgggccagc agcagggcat
     1621 ggagtgtatc ggggatctcc acgctttcgg tcaggtgagt ggtgtgccag ccgcccgagc
     1681 cgcgcaccag cacgccctga tcgacccaca tgcgcaccac ttcttccaca aaaaacggat
     1741 tgcccccggc gcggtcgagg atgcggtcgt agagggcttc tggcagggcg ctgtgatgga
     1801 aaatgttttt caacaggttg cggccatcgc ccggcgaaag cggattcagc gcaaggtcgt
     1861 ggcagttggg caggtcggca gactggcgaa tggcagtgac cagccgccag ccgggcgaat
     1921 cgggctcagc gcggctgacc gcacagagca gcacaggggc ctcattcaac agcggcagca
     1981 ggcgggtcag taaatccacc gaggaggcat cggcccagtg aatatcctca caaatcaggt
     2041 gcaggggggt gcgctctgcc agggccaata acaggcggcg cagcgcgttg agggtataag
     2101 tttgcagggt ctgcgcgtcc agagtgtgga tctgcgccag acgggcatca tccagcggca
     2161 ggccaagcag atgccgcaaa taagcggcct cggattccgc ggcgggcagc caggtatgca
     2221 gggcggcagc cacttcaatc ggagatgcgc ccggtgcggt gcccagcagc gagcgcagca
     2281 ggtcttgaat cagcgaatag gcctgctgct ggctgtaaga caggcagcgg cccagccccc
     2341 agcggagggt ctgacccgac agggcggcgc gccattcggc aatcaggcgg ctcttgccca
     2401 ggccgggctc gcccagcacc agggctgcgc cgccgcgccc gccctgcact gccgccgaga
     2461 gctgcagcag cgcggccagt tcggccaccc ggccgaccat ggggctgtcc atgcctgcca
     2521 ggccgcgcac cgagccgggg cggtctttga gccccagcag ggcataggcc tgcaccggtt
     2581 cggtcaaccc tttgaccagc accggcggca gagaagcaaa ttcaaacagg ggggcggtca
     2641 ggcggtaggt ttgcggaccc agcataatgg tgctgcgctc agaaatatct ttgagccggg
     2701 cagccagatt gactgcactg ccgatgaccg tatcgtgctg ctggccctga gagccgatgc
     2761 cgccggcgat gaccagcccg gtgttgatgc caacgtgcag gcccagggta gtgttgtgca
     2821 ccgcgttgaa ggccgccatg gcggtcatca tctccagggc ggcgcgcagc gcgcgggcgg
     2881 ggtcgtcttc gtgcgccacc ggtgcgccaa acacagccat cacctcgtcg ccgatgaatt
     2941 tatccaccac gccgccgtac ttttcaatga ccggcaccag cacatcaaag ctggcgttga
     3001 gcaggccgca aacctcttcg gcatccaact gctcggagag cgtggtaaag cccgacaggt
     3061 cggcaaacag aaccgtgacc tggcggcgct cgccgctgat gcgcccaggg cgcaccccgg
     3121 cgggcagtgg ggcggtaccc agcggtgagg tctgtgggcg gcgcagttca taggccagca
     3181 aattgggctc atcgggcgcc cggccaaggt gtaaaaagcc gctcttttcc agcacgcgct
     3241 ggctggcaat attctccggg tctacggtgg cggtgatgcc gcggatttcg ggacgggtcc
     3301 aggcccactc ggccagccgc cgcagggcct cggtcatgta accctggccc tcaacctcgg
     3361 gggcaatgcc atagcccacc tcggcgcggc cttcggcatc gggcacaccc ttgaagccga
     3421 tcaacccggc gccaaactgg gtctcggtca gcacaatcag ccagtaggtc tgccaggggt
     3481 gctgctccac cggcagcagc gccatccgtt ccagtttgat gcgcacggcg cgctgcaacg
     3541 gttctgacat gtctgcatgg agccgcgcca gccccaacgc cagacgcagc gcctcgggat
     3601 cgtcgcggta gagccgcagt tcttcagcgc tgagggcgat gagattcagc cgggcagcgg
     3661 gcagcgtcag gctcaccggg gggcctccag cacgccggtc tggatgaacc agtcataggc
     3721 cgagcggatg gcctcggaaa ccgggcgggg cggcggcagg cccagcgccg tgcgtgattt
     3781 gcgcgtgtca acgtagaaat aataacccgc cttgcggaac gtcactggcg agaagggtag
     3841 atctaaaaat ggcgacatca aacggatggg cagcgccagg cggcgcagca gcccggcggg
     3901 cagcaccagc gtgggggccg gtgcaccgac cacagcggca atttgctgca ggtaggcctg
     3961 gtgtgaaagg ttgtgtccgc cgaggatata gcgctgaccg gactggccgc gctgcagggc
     4021 tgccagatgc ccggcaacca catcctcgat gtgaacaatg ttcagcccac cctcggtgag
     4081 catgggcagg cggcgctggg ccacccgcac caccagcgag ctgctcttac gatagatatc
     4141 gcccgcgccg accaccatcg ccgggttgac ggtgaccacg tccaggcctt cggacacggc
     4201 gcgctgcacc tccatctcgg caagatattt agaataaccg tagggccagt aatccggacg
     4261 aaagttccag gtgtgggttt cgtccatcag caagggggcg gcgttccggg ctggcagggg
     4321 cggtacgccc agggctgcaa ccgaactggt atgcaccacc cggcgcacac ccgctcggcg
     4381 agcttcactt aacaaggcgc gcgtaccttc tacggtaacg gcatacatgc ggccgggctc
     4441 atcgtgcttg ccgcccagca tggccgcggc gtgaaagacc acctccatgc cagccagggc
     4501 gggagccagc gtgtcgggct gggtcaggtc gccaaccaga tgctccacgg gcagatcttc
     4561 cagcaggcgc aggttgctgc tggcgcgatg aaaagcgcgc acctggtgcc cctgctccac
     4621 cagggcgcgg cagagcgcag cgccgataaa tccggttgat ccggtgacaa gaacgttcat
     4681 ggaatgattt gattgtatcg caaatttaac gtttcgggtt tgagagcagg cggtttgcaa
     4741 ccaaaatcat atctacagcg cagtctcgaa acccttttag gctg
//
LOCUS       DAOPVS010000150         4747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_18088_length_4747_cov_61.335251, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000150 DAOPVS010000000
VERSION     DAOPVS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18088_length_4747_cov_61.335251"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKL11_09505"
     CDS             complement(<1..489)
                     /locus_tag="PKL11_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13879.1"
                     /translation="MHTITPPPWLLRFQAALLEAGDAFFRPGSSWINRTLQRLTLAAL
                     FLIGFFLWGDLLNWGQIPFEHADWYDITGPRLGFTQEALQTGQLPLHVQSATGMKDAT
                     DRFLSIPDLILSPQVVLLLWLTPGAFSLVQVLLLYALGYGGLLLLRRKWNLSLAVFIP
                     LFW"
     gene            complement(491..916)
                     /gene="arfB"
                     /locus_tag="PKL11_09510"
     CDS             complement(491..916)
                     /gene="arfB"
                     /locus_tag="PKL11_09510"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNZ13880.1"
                     /translation="MLQLADDLTLDDNDLSFETYLAAGPGGQNVNKVATAVRLRFALR
                     TSAALTDEVKARLEKLAGSRMTTEGVLVIEARRYRTQEANRQDALRRLTALIQQARVA
                     PKVRKPTKAGATARAARRSEKERRSETKRVRHYNPDEWE"
     gene            complement(943..2184)
                     /locus_tag="PKL11_09515"
     CDS             complement(943..2184)
                     /locus_tag="PKL11_09515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ13881.1"
                     /translation="MPESTPLPVDAPRPSLGRRLIQFFGLRRSMVGVLFMVILVGMGE
                     KMAERFLPIYLLALGGGTLSVGLLNGMDNLLSALYSFPGGYLSDRLGYKRALLVFNLI
                     AMFGYLIVILFPSWPAVIVGSVFFLSWTAISLPATMSLVAAVLPVQKRTMGVSLHSLV
                     RRIPMALGPMVGGALIMAYGEKDGVRLAFIVALVLGVVALILQQVMVEDKKTGGEAQR
                     NPLKLLHLITPNLRNLLISDILVRFAEQIPYAFVVIWAVKNNGITPVQFGILTTIEMV
                     TAMLIYIPIAYLADKNTKKPYVVTTFVFFTLFPLVLLFSRSFPALILAFILRGLKEFG
                     EPTRKALIMDLAPEGQKASVFGLYYLIRDVIVSVAAFSGALLWNVSPATNFLVAFGFG
                     LLGTLYFALRGKDLSSTRAAA"
     gene            complement(2229..3218)
                     /locus_tag="PKL11_09520"
     CDS             complement(2229..3218)
                     /locus_tag="PKL11_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ13882.1"
                     /translation="MNVLFIGGTGIISSACSRLAVERGLRLYLLNRGQSRRPAPEGAI
                     HLNGDIRSPESARAALGDLHFDAVVDWIAYTPDQIEADLALFRGRTDQYIFISSASAY
                     QTPPAALPVTESTVLDNPFWQYSRNKIACEERLVAAYRAEKFPITIVRPSHTYDCTLL
                     PCHGGWTVVDRMRRGLPVIVHGDGTSLWTLTHHADFARGFVPLLGNPRTIGESFHITS
                     DEWLTWDQIHRIIAHAAGAPEPRLVHVPSDWIARFDPEWGAGLLGDKAHSMIFDNTKI
                     KRLVPDYAATIPFVRGAAEIIAWYDADPARRQVDAAFNALCDRMIAAAERAQV"
     gene            complement(3215..4669)
                     /locus_tag="PKL11_09525"
     CDS             complement(3215..4669)
                     /locus_tag="PKL11_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13883.1"
                     /translation="MTKPRIFISADHGLAVVYFLQTDVLPALLEGGLEVVLLTDDGLR
                     EQIQQRFGQPGLIVEGLRFKQARQYFESYKHNAQYWLHFLRWMGGSNRINTNAMDGHL
                     RQMAYETSRRGKLLMPLVRAATWVLRRSRLARQALVNSQRRFTANLYGDLFDKYQPQL
                     VVASTPGWRLDRFLLREAAARGVHTAAAIVGWDNPSSYRLPGAPMDFVTCWSEIQKQE
                     LVLGSDWAPERVNIGGIPSYDGYFRKTWQMPREDYFALHGLDPNRKLLAYASSFVTFA
                     PNYPNIEALARLVSSDALSEPCQLLIRLHPNHFMPGSLYEGEASRVRALIRDLPHVHL
                     VEPVPLGGELGYYSGEDMPEKSSMMAHADIFLTVYSTMVVETAIHDRPIIGVCLDVPG
                     GWNTPGKFSLPLAQIAEWPTHLRFRQAGAGRVAMTAEELRESVNFYLLNPDADRPARQ
                     RFIRDEVTFTDGGAGLRTGEALRKLALEGRWRDA"
BASE COUNT          958 a         1498 c         1484 g          807 t
ORIGIN      
        1 ccagaacagc gggatgaaca ccgccagcga gaggttccat ttgcggcgca gcagcagcag
       61 cccgccgtaa cccagcgcat agagcagcag cacctgcacc aggctgaacg cgcccggcgt
      121 gagccagagc agcaagacca cctgcggtga gaggatcagg tcggggatgg acagaaagcg
      181 gtcggtggcg tctttcatgc ccgtggccga ctgcacatgc agcggcagtt gcccggtttg
      241 cagcgcttcc tgcgtgaagc ccagccgcgg cccggtgata tcgtaccagt cggcgtgttc
      301 aaacggaatc tgcccccagt tgagcaggtc gccccacagg aagaatccaa tcaaaaacag
      361 cgccgccagc gtcagccgct ggagagtgcg gttaatccaa ctggagccgg gtcggaaaaa
      421 ggcatcgccg gcttccagca gtgcggcctg aaagcggagc agccaggggg gtggggtgat
      481 tgtgtgcatg ttattcccat tcatccgggt tgtagtggcg gacgcgcttg gtctcgctgc
      541 ggcgctcttt ttcactgcgg cgggccgccc gcgccgtggc tccggctttg gtgggtttgc
      601 gcacctttgg cgccacccgg gcctgttgaa tcagtgcagt caggcggcgc agggcatcct
      661 gccggttggc ctcctgtgtg cggtagcggc gcgcctcaat gaccagcacc ccctcggtgg
      721 tcatccggct gccagccagc ttctccaacc gggctttgac ctcgtcggtc agggcggcgc
      781 tggtgcgcag ggcaaaacgc agccgcacgg ctgtcgccac cttgttcaca ttctgcccgc
      841 caggtccggc agccagatag gtctcgaagc tcaggtcgtt atcatccagg gtcaggtcat
      901 ctgccagttg tagcatggct taaattgtac ccgaagaatg gatcaggcgg ctgcccgcgt
      961 ggaggacaga tctttgccgc gcagcgcaaa gtagagcgtg cccagcagcc caaagccaaa
     1021 ggccaccaga aagttggtcg ccggtgagac gttccacagc agcgcgcccg aaaaagccgc
     1081 caccgagacg atcacatcgc ggatcaggta atacagcccg aacacgctgg ccttctgtcc
     1141 ctctggcgcc aggtccatga tcagcgcctt gcgggttggc tcgccaaact ctttcagccc
     1201 gcgcaggatg aaggccagaa tcagcgccgg gaacgagcgg ctgaacagca gcaccagcgg
     1261 gaagagcgtg aaaaacacaa acgtggtcac cacatagggc tttttggtgt ttttatcggc
     1321 cagataggca atcgggatgt aaatcagcat cgccgtgacc atctcgatgg tggtcagaat
     1381 gccgaactgc accggcgtga tgccgttgtt cttgactgcc cagataacca caaaggcata
     1441 ggggatctgc tcggcgaagc gcaccagaat atccgagatc agcaggttgc gcaggtttgg
     1501 cgtgatgagg tgcagcagct tcagcgggtt gcgctgcgct tcgccgccgg tctttttgtc
     1561 ctccaccatc acttgctgta aaatcagcgc cacaaccccc agcaccagcg ccacaatgaa
     1621 cgccagccgc acaccgtcct tctcgccgta ggccataatc agtgcgccgc ccaccatggg
     1681 ccccagcgcc atggggatgc gccgcaccag cgagtgcagc gagacgccca tggtgcgctt
     1741 ctgcaccggc agcaccgccg ccaccaggct catcgttgcc ggcagcgaga tggccgtcca
     1801 cgagaggaag aacaccgagc ccacaatcac cgccggccag gacggaaaca ggatcacaat
     1861 cagatagcca aacatggcaa tcaggttgaa gaccagcagg gcgcgtttgt aacccagccg
     1921 gtcgctcaga tagccgcccg ggaaggagta cagcgcgctc agcaggttat ccatgccgtt
     1981 gagcaggccc accgagagcg taccaccgcc cagcgccagc aggtagatgg gcaaaaaccg
     2041 ctcggccatc ttctcgccca tgccgaccag aatgaccata aacagcaccc ccaccatgct
     2101 gcggcgcagg ccaaaaaact gaatcaaacg gcggcccagg ctcgggcggg gggcatcaac
     2161 agggagggga gtagattcag gcatttgggc ttctcgattt tagattgtgg atatagattc
     2221 ggtcgggcct acacctgtgc gcgttctgcc gcggcaatca tccggtcgca cagggcgttg
     2281 aaggccgcgt ccacctgacg gcgggcgggg tcggcgtcat accaggcgat gatctccgcc
     2341 gcgccgcgca caaacgggat ggtcgcggca taatcgggca ccagccgctt gattttggta
     2401 ttgtcaaaaa tcatgctgtg cgccttgtcg cccagcagcc ccgcgcccca ctcggggtca
     2461 aagcgggcga tccagtccga aggcacatgc accaacctcg gctccggcgc gcccgcagca
     2521 tgagcaatga tccggtgaat ttgatcccag gtcagccact catccgaggt gatgtggaag
     2581 ctctcgccga tggtgcgggg gttgcccagc agcggcacaa agccgcgcgc aaaatcggcg
     2641 tggtgcgtca gcgtccacag ggaagtgccg tcaccatgca caatcaccgg caggccgcgg
     2701 cgcatccggt ccaccaccgt ccagccgccg tgacagggca gcagggtgca gtcatatgta
     2761 tgcgaggggc gcacaatggt gatggggaat ttctcggcgc ggtaagccgc caccaggcgc
     2821 tcttcgcagg caatcttatt gcgggagtac tgccagaagg ggttatccag tacagtcgat
     2881 tccgttaccg gcagcgcagc cggcggcgtc tgataggctg aagccgaact gataaagatg
     2941 tactggtcgg tgcgcccgcg aaacagcgcc aggtctgcct caatctggtc gggggtgtag
     3001 gcaatccagt ccaccacggc gtcaaaatgc aggtcgccca gcgcggcgcg ggccgattca
     3061 gggctgcgaa tgtcgccgtt gaggtgaatg gcgccttccg gtgccgggcg cctggattgg
     3121 ccgcggttga gcaaatacag cctgaggccg cgttccaccg ccagccggga gcaggccgat
     3181 gagatgatcc ccgtgccgcc aatgaacaga acgttcatgc gtcccgccag cgcccttcca
     3241 gggccagttt gcgcagcgcc tcgccggtgc gcagtccggc gccgccatcg gtgaaggtca
     3301 cctcatcccg aatgaagcgc tgccgcgccg gacgatcagc atcgggattc agcaggtaaa
     3361 aattgaccga ctcgcgcagt tcttcggcag tcattgccac gcgcccggcg cctgcctgcc
     3421 ggaagcgcag gtgggtgggc cactcggcaa tctgtgccag cggcagcgag aacttgcccg
     3481 gcgtgttcca gccgcccggc acatccaggc agacgccgat gatcgggcgg tcgtgaatgg
     3541 cggtctcaac caccatggtc gaatagacgg tcaggaaaat gtcggcgtgc gccatcatgc
     3601 ttgatttttc gggcatatcc tcgccggagt agtagcccag ctcaccgccc agcggcaccg
     3661 gctccaccag atgcacatgc ggcaggtcgc ggatcagcgc ccgcacccgg ctggcctcgc
     3721 cctcatagag cgagccgggc ataaagtggt tggggtgcag ccggatcagc agttggcagg
     3781 gttctgacag cgcatcgctg ctcaccagcc gcgccagcgc ctcgatgttg gggtagttgg
     3841 gcgcaaaggt cacaaaactg ctcgcatagg ccagcagctt gcggttgggg tccagcccgt
     3901 gcagggcaaa ataatcttcc cggggcatct gccaggtttt gcggaaatag ccgtcatagc
     3961 tgggaatgcc gccgatattc acccgctccg gcgcccagtc tgagccgagc accagctcct
     4021 gcttctgaat ctccgaccag caggtgacga aatccattgg cgcgccgggc agccggtaac
     4081 tggaggggtt atcccaccct acaatggccg cggcagtgtg caccccccgc gccgccgcct
     4141 cgcgcagcaa aaagcggtcc agccgccagc cgggcgtgct ggcaaccacc agctgcggtt
     4201 gatatttgtc aaacaggtcg ccatagaggt ttgcggtgaa gcggcgctgc gaattgacca
     4261 gcgcctggcg ggccagccgc gagcggcgca gcacccaggt ggccgcgcgc accagcggca
     4321 tcagcagctt gccgcgccgc gaggtctcgt acgccatctg gcgcaggtgc ccgtccatcg
     4381 cattggtgtt gatgcggttt gagccgccca tccagcgcag aaaatgcagc cagtactgag
     4441 cgttgtgttt gtagctttca aaatactggc gcgcctgctt gaagcgcagc ccttccacaa
     4501 tcagtccggg ttgaccaaag cgctgttgaa tctgctcgcg cagcccgtcg tcggtcagca
     4561 gcaccacctc caggccgcct tccagcagcg cgggcagtac atcggtttgt agaaaataga
     4621 ccaccgccag accatggtcg gcgctgatga aaatgcgggg tttcgtcaca gggtgcgggt
     4681 ctccttcatc atccggcatt accggtcata ataccagcga aaaatagcat catacagcgc
     4741 cagcagt
//
LOCUS       DAOPVS010000151         4736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_18152_length_4736_cov_65.564196, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000151 DAOPVS010000000
VERSION     DAOPVS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18152_length_4736_cov_65.564196"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1258)
                     /locus_tag="PKL11_09530"
     CDS             complement(212..1258)
                     /locus_tag="PKL11_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNZ13884.1"
                     /translation="MEFANLVPLVEATRGPIVESIMCGAAAIVDAQNRLVASLGDPDT
                     FTYPRSTAKPLQVLPFVERGGLEHFGITPRELALMCASHAGTDDHAAVAESIQRKVGV
                     KESDLLCGMHPPYDKDTLYRMIRTGEESRPNRHNCSGKHSGMLAHARLRGWPIEDYIN
                     TEHPLQKTIIQTFAEMTSFPAEKLGIGVDGCSAPVFALPLRNFALGFARLCDPAGLAP
                     ERAAACQKITQAMMSHPDMVSGPGRFDTRLMEVCQGKLIAKGGAEGYQGVGLLPGALG
                     PGSPALGIAFKAADGDLGDRVRPLVTIELLRQLGVLGPAEIEALAGFATRPLYNWRKL
                     PIGELRPVFKIQVG"
     gene            complement(1339..2196)
                     /locus_tag="PKL11_09535"
     CDS             complement(1339..2196)
                     /locus_tag="PKL11_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13885.1"
                     /translation="MQQIASHVYIETAYPGVTLGAINWSHGLILIDAPLRPEDARSWR
                     AALLNLGGGVDRLLINLDPHYDRTLGARALECTVLGHEKMMQTYRSRSASLKSQGFET
                     GAEWELHNNLTGIRWAPPEITFTDTLHIHWNGEPLVLTYRPGPAQGAIWAELPESKVV
                     FVGDAVVAHQPPFLAQANLPQWVETLQALLEPQYREYLIVSGRGGLVTAENVRAQLAW
                     LERLHTDMEDLAERKVMPEQTDKLAPALLKALNPPAEQEACYRQRLRWGLHQYYLRHY
                     RPTNEELEE"
     gene            complement(2207..3778)
                     /locus_tag="PKL11_09540"
     CDS             complement(2207..3778)
                     /locus_tag="PKL11_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ13886.1"
                     /translation="MSPDRTRASLELLLNISRELAASLDLNTVLNRVLLLSARNVGAE
                     RGSLVALDEYLRPVEAAIIVEERFVPHTLQQLQGIVSHGLAGWVLRNQKAALVLNSRE
                     DDRWLRRPDDEAEGSGPKAAICVPLLAQDEIVGVMTMVHPEPGTFNEDHLALLQAIGD
                     LAGIAIRNAQLYHSLQTVNQLYRELFEDSIDPLIITDWNGRILQANRRAAEDMGTPAH
                     ELVGANISTLHTVAWDVVGPQYGALRGGVMVSYESILHRVRRRDCPVQVHVRAINYSG
                     EDSLQWIIRDITERKQLDSLREDLAAMIYHDLRSPLSNIVSSLDILTSLTPVQGDASA
                     AAVMQIAVRSTERMQRLINSLLDINKLESGQPIASRQAVDSKELVHAAVEAVLPFVDG
                     KQIKLKVSLPAELPAIHADEDMVRRVLINLMENAIKFTPQEGGLQVGAAAEPDKVRFW
                     VQDTGPGIPPEARQLIFEKFTRLQVERSPKGLGLGLAFCRLAVQAHGGRIWVEGEPEQ
                     GSCFNFTIPIAPPQP"
     gene            complement(3881..4672)
                     /locus_tag="PKL11_09545"
     CDS             complement(3881..4672)
                     /locus_tag="PKL11_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNZ13887.1"
                     /translation="MLTFRELVSGLRSLGLDRRTPVIMHASLSAFGQVHGGGETLLGA
                     LITAFPLIMAPTFTYKTMLIPEDGPADNGLDYGSGRDVNRMAEFYHPDMPADKLMGTL
                     PEALRQRTAAQRSMHPILSFSGIGVPELLKAQTIEEPLAPIRALAEQDGWVLLLGVNH
                     TVNTSIHEAERQAGRQQFIRWALTPQGVCECPGFPGCSLGFEQAAAALMDITRTVALG
                     DSRVQALPLQPMFEIIGQMLRADPLALLCSLPDCERCAAARARLG"
BASE COUNT          886 a         1464 c         1481 g          905 t
ORIGIN      
        1 ctcacgttga aggtcgtcaa ccgctggggc aatgaaacca cctgggatga taccttcacg
       61 gttgatctac cgattcagaa gctgcacctg ccgttgattc aacgctaggc tcggcgcttt
      121 tctggccgtg ggagaacttc cccctgccct aaacgccggt aagaacgtct ggctgtctgc
      181 caacgctctt accggcgttt gatgatctga tctaaccgac ctgaatcttg aataccgggc
      241 gcagttcgcc aatggggagc ttgcgccagt tgtacaatgg gcgggtggca aagccagcca
      301 gcgcctcaat ttctgcaggg cccaacacgc caagctgccg cagcagctca atggtcacca
      361 gcgggcgcac ccggtcgccc aggtcgccat cggcagcctt gaaggcaatg cccagcgccg
      421 gactgcccgg gcccagcgcg ccgggcagca ggccaacgcc ctggtagccc tcggccccgc
      481 ccttggcgat cagcttgccc tggcacactt ccatcaggcg ggtatcaaac cggccggggc
      541 ccgaaaccat gtccggatgg gacatcatgg cctgggtaat cttctggcag gcggcggcgc
      601 gctcgggcgc cagtccggcg gggtcgcaca ggcgggcaaa gcccagcgca aaattgcgca
      661 gcggcagggc aaacaccggc gccgagcagc catctacgcc aatacccagc ttttcagccg
      721 gaaaactggt catctcagca aaggtctgga tgatggtttt ttgcagcgga tgctccgtgt
      781 tgatgtagtc ttcaatcggc cagccgcgca gccgggcatg cgccagcatg ccgctgtgct
      841 tgcccgagca gttgtgccgg ttggggcggc tttcttcgcc cgtgcggatc atccggtaaa
      901 gcgtgtcttt atcatacggc gggtgcattc cgcagagcaa atcgctctct ttaacgccca
      961 ccttgcgctg gatgctttca gccacggcgg catggtcatc ggtgcccgca tgggaggcgc
     1021 acatcaaggc cagctcgcgg ggggtgatgc caaaatgctc caaaccgccg cgctcaacaa
     1081 agggcagcac ttgcagcggc ttggccgtgg agcgggggta ggtgaaggtg tcgggatcgc
     1141 ccaggctggc aaccagccgg ttctgggcat ccacaatggc agcagccccg cacatgatgg
     1201 attcgacgat ggggccacgc gtggcctcaa ccagcggcac aagattcgca aattccattc
     1261 cgttatcctt ttgtaaataa attaacctgt ccccgatcac tcatttgaaa gcggtgacca
     1321 gggacagatt atagacgctt attcttccag ctcttcgttg gtggggcggt agtggcgcag
     1381 gtaatactgg tgcagccccc agcgcagccg ctgacggtaa caggcctctt gttcggcggg
     1441 cgggttcagg gctttcaaca gcgccggggc cagtttgtcg gtttgctcgg gcatcacctt
     1501 gcgctcagcc aggtcttcca tgtcggtgtg caggcgctcc agccaggcca gttgggcgcg
     1561 cacgttctcc gccgtgacca gcccgccgcg cccgctgacg atcagatact cgcggtactg
     1621 cggctccagc agggcttgca gggtttccac ccactggggc aggttggcct gcgccaggaa
     1681 gggcggctga tgcgccacca cggcatcgcc cacaaacacc acctttgatt cgggcagttc
     1741 ggcccagatg gcgccctgcg ccgggccggg gcggtaggtc agcaccagcg gctcgccatt
     1801 ccagtgaatg tgcagggtgt cggtaaaggt gatttccggc ggggcccagc gaatgccagt
     1861 caggttattg tgcagttccc attcggcgcc agtctcgaag ccctgagatt tgaggctggc
     1921 cgacctgctg cggtaagtct gcatcatctt ttcgtggccc agcacggtgc attccagggc
     1981 gcgggcgccc agggtgcggt cgtagtgcgg gtccaggttg atgagcagcc ggtccacgcc
     2041 gccgcccagg ttgagcagcg cggcgcgcca cgagcgggcg tcttcggggc gcaggggggc
     2101 gtcaatcaaa atcagcccat gcgaccagtt aatggctccc agggtcacac ccggataagc
     2161 ggtttcgatg taaacgtgcg aagcgatttg ttgcatacct taatccttag ggttgtgggg
     2221 gagcgatggg aatggtgaag ttgaaacaac tgccctgttc gggttcgcct tccacccaga
     2281 tgcgcccgcc atgcgcctgc acggccaggc ggcagaaagc caaccccagc cccaggccct
     2341 tgggagatcg ttcgacctgc aggcgggtaa atttttcaaa aataagctgc cgggcttcgg
     2401 gcggaatgcc cggccccgta tcctggaccc agaagcgcac cttgtcgggc tctgcggctg
     2461 cgccgacttg caggccgcct tcctgggggg tgaacttgat ggcgttttcc atcaggttga
     2521 tcaagacccg gcggaccatg tcttcgtcgg catggatggc gggcagttcg gccggcagcg
     2581 aaaccttgag cttgatctgc ttgccatcta cgaagggcag cacggcctcc accgcggcgt
     2641 gcaccagttc tttggaatca accgcctgcc ggctggcaat cggctgcccc gattccagct
     2701 tgttaatgtc gagcagtgag ttgatcagcc gctgcatgcg ctcggtggag cgcacggcaa
     2761 tctgcatcac cgccgccgcc gaggcatcgc cctgcacggg cgtcagcgag gtgagaatat
     2821 ccaggctgga gacgatgttg gaaagcggcg agcgcaggtc gtggtagatc atcgcggcca
     2881 ggtcttcgcg cagcgaatcg agctgtttgc gctcggtaat gtcgcggata atccactgca
     2941 gcgagtcctc gccgctgtaa ttgatggcgc gcacatgcac ctgcacgggg cagtcacgcc
     3001 gcctcacacg gtgcagaatc gattcgtagc tcaccatcac gccgccgcgc agcgcgccgt
     3061 actgcggccc caccacatcc caggccacag tatgcagcgt gctgatgttg gcgccgacca
     3121 gctcgtgagc cggtgtgccc atgtcttcgg cggcgcggcg gttggcctgc aaaatgcggc
     3181 cgttccaatc ggtgataatc aacgggtcaa tgctgtcttc aaacagctca cggtagagct
     3241 ggttgaccgt ttgcagcgag tgataaagct gggcgttgcg aatggcaatg cccgccaggt
     3301 cgccaatggc ctgcagcagc gccaggtgat cttcgttgaa ggtgcccggt tccgggtgca
     3361 ccatcgtcat cacgccgaca atttcatcct gcgccagcag gggcacgcag atggcggcct
     3421 tggggcccga gccctcggct tcatcatcgg ggcggcgcag ccagcggtca tcctcgcgtg
     3481 agttgagcac cagcgcggct ttttgattcc gcagcaccca gcccgccagc ccgtgcgaga
     3541 caatgccctg taactgctgg agggtgtgcg gcacaaaccg ctcttcaacg ataatggcgg
     3601 cctcaacagg ccgcagatac tcatccaacg ccaccaggct gccgcgctct gcgcccacat
     3661 tgcgggctga aagcagcagc acccggttga gcacggtatt taaatccaaa gaagccgcca
     3721 actcacggct gatgtttaac aataattcca gtgatgcacg cgttcgatcc gggctcattg
     3781 gcagacctcc ctattattct accctaaatc cgaattagcg atgatgtgga ttaagaccgg
     3841 ttttggggtg atttatcgcc cggaaagggt tgggcatccc tcagccgagg cgggcacggg
     3901 ctgcggcgca gcgctcgcag tcgggcaggc tgcacagcag cgccagggga tcagcgcgca
     3961 gcatttgccc gatgatttca aacatgggct gcaggggcag cgcctgcaca cgcgaatcgc
     4021 ccagcgccac cgtgcgggtg atgtccatca gtgcggcagc agcctgctca aagcccagcg
     4081 agcagcccgg aaagcccggg cattcgcaca cgccctgggg ggtcagcgcc cagcggatga
     4141 actgctgccg cccggcctga cgttctgctt cgtggatgct ggtattgacg gtgtggttga
     4201 cgcccagcag cagcacccag ccatcctgct cagccagggc gcggatgggc gccagcggct
     4261 cttcgatggt ttgggccttc agcagttcgg gcacaccaat gccgctgaac gatagaatgg
     4321 ggtgcatgga gcgctgggct gccgtgcgct ggcgcagcgc ttcgggcagg gtgcccatca
     4381 gcttatcggc gggcatgtcg gggtgataaa actcagccat gcggttcaca tctcgcccgc
     4441 tgccataatc caggccgtta tcggcggggc cgtcttcggg gatcaacatg gtcttgtagg
     4501 taaaggtcgg cgccataatc agtggaaaag ccgtaatcag cgcgccgagc agcgtttcgc
     4561 cgccgccgtg cacctgtcca aatgccgaca gcgaagcgtg cataatcacc ggcgtgcgcc
     4621 ggtccaggcc caagctgcgt aatccactga ccagttcccg aaaagtaagc atcgccatca
     4681 ctccattttc caccgcaggt taatccctat tatacgcgag aatggttttt tacgtt
//
LOCUS       DAOPVS010000152         4733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_18163_length_4733_cov_54.013895, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000152 DAOPVS010000000
VERSION     DAOPVS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18163_length_4733_cov_54.013895"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKL11_09550"
     CDS             <1..692
                     /locus_tag="PKL11_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNZ13888.1"
                     /translation="PRPLEEVRQFSESITRYLLGEGARLIVVACNTASAAALHALRAT
                     FPDIPFVGMEPAVKPAAEQTVTGVVGVLATPATFQGALYASVVERFGQGVTLLQNTCP
                     GLVQQIEAGNLTGTETRQILEQALQPMLAQGIDTVVLGCTHYPFVIPLVETIAGPSVR
                     VIDPAPAVARQTARLIEQYQLSGAGNVEITPSRFITTGAVEKLAALLPELLGTDYVCE
                     GLSWRGNELGA"
     gene            755..1279
                     /locus_tag="PKL11_09555"
     CDS             755..1279
                     /locus_tag="PKL11_09555"
                     /inference="COORDINATES: protein motif:HMM:NF022734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HNZ13889.1"
                     /translation="MPQEIDLDPVSRLTTDAIGQPGQRVFYLQGAAGEQVISLVVEKI
                     QIQTLAIGVEEFLAEIGSQFPDLQAAEAQYHEAEMRIVPPVDVLFRVGELGLAYDAER
                     DLICLVAREILTGEMSTEDAAVVRFWCSRTQLRRMASWGIELTNRGRAICPQCGEPMD
                     PAGHFCPKKNGHKH"
     gene            1263..2003
                     /locus_tag="PKL11_09560"
     CDS             1263..2003
                     /locus_tag="PKL11_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNZ13890.1"
                     /translation="MGTSIESEAEVLNILRRGDVELEGQFARGSNYTFLARVSLDGQS
                     LLTVYKPIRGEQPLWDFPHRTLARREAAAYCLSEALGWGLVPPTIYRRRKAPLGPGSL
                     QVFIEHDPNRHYFSFSPEEHERLRPAAVFDLLLNNADRKGGHLLLDAQEHLWLIDHGL
                     CFNIEPKLRTVIWDFAGQTIPDDLCGDLARVLAMLNGVESALRAELTALLSAAEVSAL
                     AERIRSLLEDQRFPLPPGSRRPYPWPPI"
     gene            2025..3062
                     /locus_tag="PKL11_09565"
     CDS             2025..3062
                     /locus_tag="PKL11_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNZ13891.1"
                     /translation="MPRTYSLLLVGFGNVGQAFARLLLRKERLLEDEFGCKFRVTGIA
                     TGRHGRALDSAGINLPRALELANAGQSMSLLSAQEAPTDMAEFIHAATADVLFENTPV
                     NHRTGQPAADYLRLGLEKGMHAITANKGPVVHAYRELTALAAQRGRRFLFESAVMDGA
                     PIFSLFRGPLPAAEVTGFQGILNSCTNLLLGMMEEGKTLDEAIVYGQSIGITETDPSA
                     DVDGWDAAIKVAALTTVIMGIPLKPQDVDREGIREITAQQIADARQNGKRWKLVCTAK
                     RQGPQMITARVRPEQIAADSPLYAINGTSSYVQFESDVLPGLGIVESNPGPETTAYGL
                     LADLLNALRGM"
     gene            3066..3668
                     /locus_tag="PKL11_09570"
     CDS             3066..3668
                     /locus_tag="PKL11_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13892.1"
                     /translation="MIEAYWIAGCTASSVAEAAAQTLAKAGVKPEWIDEAHQIGAVMD
                     LDSPAQKYHWPVVSPGDHRLVNLLVRSLAARECGLMLVGQTQEGQSALALLASAAAVG
                     GRNILARARLLPLSGHRPPHDFFNHLPEALTEVEVESEQVAWVDTPEPPFATAEWVQP
                     EQPGAIFRLNRLAEAVQGKVQSAGLMLSPMAGLATLIQRT"
     gene            3677..>4733
                     /gene="folP"
                     /locus_tag="PKL11_09575"
     CDS             3677..>4733
                     /gene="folP"
                     /locus_tag="PKL11_09575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNZ13893.1"
                     /translation="MHTLYLALGSNLGNRRGNLQAALDALSPQIHWRRSSPIYETPPW
                     GFTDQPAFLNMVVEAETDLEPAELLKTLKDIEARVGRTPSFRYGPRQIDLDILFYDDL
                     VVDTPELGIPHPRLAERAFVLQPLADLNPGLSHPQLKYTVNELLKNVDTSGMKLFDPP
                     CRLEWGKRTYVMGIINVTPDSFSGDGLLGAAASVEAAVEQARRFVAAGADILDIGGES
                     TRPGSEPVDADEEMGRILPIIRALSMAGVNAIISVDTYKAETAEAALRAGAHWINDVW
                     GLRADPRLAEIAARRGATVVLMHNRSTPTNVEIQERLGGRYVGMDYTDLLTDVCNELQ
                     ESAAQAKAAGIPDHQIIL"
BASE COUNT          966 a         1234 c         1570 g          963 t
ORIGIN      
        1 ggccgcgccc gctggaagaa gtgcgccagt tttctgaaag cattacccga tatttgctgg
       61 gcgagggcgc gcgtctgatt gtagtggcgt gcaacacggc gtcagcggcg gcgctgcatg
      121 ccctgcgagc cacgttcccg gatattccgt ttgtgggcat ggaacccgcg gtcaaaccag
      181 ccgcagaaca gactgtgacc ggcgtggtgg gggtgctggc aaccccagcg accttccagg
      241 gggctttgta tgcctcagtg gttgaacggt ttgggcaggg cgtgaccctg ctgcaaaaca
      301 cctgccctgg actggtgcag caaattgaag ccgggaactt aaccggaacg gagacacgcc
      361 agatattgga acaggcactg caacccatgc tggcacaggg gatcgatacg gttgtgctgg
      421 gatgcaccca ttacccgttt gtcattccgc tggttgagac gattgccggg ccaagcgtcc
      481 gggtgattga cccggcaccg gcagtggcga gacagacggc gcggctgatt gagcagtacc
      541 agttgtcagg cgctggcaat gtggaaatca ccccgtcacg gttcattacc actggagcgg
      601 ttgaaaagct ggcggcgctg ctgcccgaac tgctgggcac ggattatgtc tgtgagggac
      661 tgtcgtggcg gggaaacgaa ttgggggcat aaagttcggg gcgctttctg gtatacttgg
      721 catggagcca attatctaat ccaaggatgc cgctatgcca caggaaattg accttgatcc
      781 cgtcagcaga ttgaccaccg atgccattgg acagccgggg cagcgtgttt tttatcttca
      841 aggcgcggct ggcgagcagg tgatcagtct ggtggtggaa aaaatccaga ttcaaaccct
      901 ggcaattggg gtagaagaat ttttagccga aattggcagc cagttccctg acctgcaggc
      961 agcagaggcg caataccatg aagctgaaat gcggattgtg ccgccagtgg atgtgctctt
     1021 ccgggttggt gagttggggc tggcctatga cgctgagcgg gatttgatct gcctggttgc
     1081 gcgggaaatt ctcacggggg agatgtcaac tgaagatgcg gcggtggtcc gcttttggtg
     1141 cagccgaacc caactgcgcc gtatggcgag ctggggcatt gagttaacca accgcgggcg
     1201 ggccatttgc ccgcagtgcg gagagccgat ggatccggcg gggcattttt gcccgaagaa
     1261 aaatgggcac aagcattgag tctgaagcag aggtgctgaa tattctgcgc cgcggcgacg
     1321 tggaattaga ggggcagttt gcgcgcggat caaactatac cttcctggca cgggtttcgt
     1381 tggatgggca gagcctgctg acggtctata aacccattcg cggggagcag ccgctgtggg
     1441 attttccaca ccgcacgctg gcgcggcggg aagccgcagc ctactgcctg agcgaagcgc
     1501 tgggctgggg cctggtgccg cccaccatct accggcggcg caaggcgccg ttggggccag
     1561 gatcgctgca agtttttatt gagcatgacc ctaaccggca ctactttagt ttcagcccgg
     1621 aagaacacga gaggctgcgc ccggctgcag tttttgacct gctgctgaac aacgctgacc
     1681 gcaagggggg gcatctgctg ttggatgccc aggaacacct gtggctgatt gaccatgggt
     1741 tatgctttaa tattgaaccc aagctgcgca ccgtcatctg ggattttgcc ggacagacga
     1801 taccggatga tctctgtggt gatctggcgc gcgtgctggc aatgctgaac ggcgttgaaa
     1861 gcgccctgcg cgccgaactg accgcgctgc tctcggctgc cgaggtgagc gcactggctg
     1921 aacgtattcg ttctctgctg gaggaccagc gttttccgct gccgcccggc agccggcgcc
     1981 catatccctg gccccccatt tagtacagaa agaagggaat ttctatgccc cgtacctact
     2041 cactcctttt agttggtttt ggtaatgttg ggcaggcttt tgcccggctg ttgctgcgca
     2101 aggaacgcct gctggaagat gaatttggct gtaagttccg ggtgacgggc atagccaccg
     2161 ggcggcacgg ccgcgcgctt gattccgccg ggattaacct gccgcgtgcg ctggaactgg
     2221 ccaatgccgg acaatcgatg agtctgctct cagcgcagga agccccgaca gacatggcag
     2281 aatttattca cgcagcaaca gccgatgtgc tgtttgagaa tacgccggtt aaccaccgca
     2341 ccgggcagcc tgcggcagac tatctgcggc tggggttgga aaaggggatg catgccatta
     2401 ccgccaataa agggccagtg gtgcatgctt accgggaatt gacggcgctg gcggcgcagc
     2461 gaggccggcg gtttctgttt gaatcggctg tgatggacgg cgcaccgatt ttttcgctgt
     2521 tccgggggcc attgcccgca gcagaggtga ccggttttca gggcattctc aattcgtgca
     2581 ccaacttgct gctggggatg atggaggaag gtaagacgct ggatgaggcg attgtttacg
     2641 ggcagtccat tggcattact gagaccgacc ccagcgcaga tgtggacggc tgggatgccg
     2701 ccatcaaggt ggcggcgctg acgaccgtca ttatgggaat cccgctaaaa ccacaggatg
     2761 tggaccgcga agggattcgg gagattaccg cgcagcaaat tgcagatgcc cgccaaaatg
     2821 ggaagcgctg gaaactggtt tgcacagcca aacggcaggg gccgcaaatg atcaccgcac
     2881 gggttcgccc ggagcaaatt gcggcagatt cgccgctgta tgccatcaac ggcaccagct
     2941 cttatgtgca gtttgagagt gatgtgctgc cggggctggg cattgtggaa agcaaccccg
     3001 ggccggagac taccgcctat gggctgctgg ctgacctgct gaacgcactg cggggcatgt
     3061 aaaacatgat tgaggcgtac tggattgctg gctgcacagc cagttcagtg gcagaggcgg
     3121 cggctcagac gctggcaaag gccggtgtaa agccagagtg gattgatgag gcgcatcaga
     3181 taggcgcggt gatggatttg gacagcccgg cgcaaaagta tcactggccg gtggtatccc
     3241 ctggcgatca tcgattagtc aatttgttgg tgcgcagcct ggcagcgcga gagtgcggcc
     3301 tgatgctggt tggacaaact caagaggggc aatctgctct ggcgctgctg gcttcggcgg
     3361 ctgctgtagg tgggcggaat attctagcgc gggcacggtt attaccgtta agcgggcacc
     3421 gccccccgca cgattttttc aatcatctgc cagaggcgct gaccgaggtt gaggttgaaa
     3481 gcgagcaggt ggcctgggta gatacaccgg agccgccatt tgccaccgcc gaatgggtac
     3541 agccggaaca accgggagcc atttttcggc ttaaccgcct ggcagaggcg gtacagggca
     3601 aggtacagtc ggcaggcttg atgctcagcc cgatggcagg gctggcaacg ttgattcaaa
     3661 ggacgtgagg tgagcgatgc atacgcttta tctggcactg gggtccaatc tggggaatcg
     3721 acgggggaac ttgcaggcag cgctggatgc gttatcgcca cagattcact ggcggcgctc
     3781 atcgccaatc tatgaaacgc ccccctgggg ctttaccgac cagccggcat ttctcaacat
     3841 ggtggttgag gccgaaaccg accttgagcc agcggagtta ttgaaaaccc ttaaggatat
     3901 tgaggcgcgc gtggggcgga cgccaagttt ccgctatggg ccgcggcaaa ttgacctgga
     3961 cattctattc tatgatgacc tggttgtgga tacaccagag ttgggcattc cccacccccg
     4021 gctggctgag cgggcgtttg tactgcagcc gctggccgac ctaaaccccg gattaagcca
     4081 tccgcagttg aagtacaccg tgaatgagct gctgaagaat gttgatacca gcgggatgaa
     4141 gttgtttgat ccgccatgcc ggttggaatg gggcaaacgc acttatgtga tgggcatcat
     4201 caacgtcacg ccggatagtt tttcggggga tggcctgctg ggagcagccg ccagcgtgga
     4261 ggctgcggtt gagcaagcgc ggcggtttgt ggcggcgggg gccgatattc tggatattgg
     4321 cggcgagagc acccgaccag ggtcagagcc ggtggatgcg gatgaggaaa tggggcgcat
     4381 cctgccgatc atccgagcgc tgtcgatggc tggggtgaat gccatcatct cagtcgatac
     4441 ttataaggca gagacggctg aagccgcgct gcgcgcaggc gcgcactgga tcaacgatgt
     4501 ctgggggctg cgagccgatc cccggctggc agagattgcc gcgcggcgcg gggcgacggt
     4561 agtgttgatg cacaaccgca gcacaccgac caacgtggaa attcaggaac gcctgggagg
     4621 gcgttatgtc ggcatggatt acaccgactt gctgaccgat gtgtgcaatg aactgcagga
     4681 gagcgcggca caggcaaagg cggcgggtat tcccgaccat cagattatcc tcg
//
LOCUS       DAOPVS010000153         4535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_19196_length_4535_cov_54.931250, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000153 DAOPVS010000000
VERSION     DAOPVS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19196_length_4535_cov_54.931250"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PKL11_09580"
     CDS             <1..1409
                     /locus_tag="PKL11_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNZ13894.1"
                     /translation="LMYHVGVRYGSPEGQEFAAQVMEFVRYQAMQTSVELAKVRGAFP
                     AIQGSIYDPDDMQWEPPQPLMPYDADWGRPALDWEALTDNIRQNGIRNAAQTTVAPTG
                     TIATVAGCEGYGCEPVFALAYIRHVNDNGKDLQLNYASPLFEQALIEAGLEAGAREDI
                     VEAVMAAGSCQTVETVPARLRDVFVVSGDISAEEHVHMQAAMQAFVDNSLSKTINFPE
                     GATTDEVSKAYMLAWELGCKGITVYVTGSREKVVLETRSTADKKQPVAETPRQLELPA
                     AQPTIWQESKKPRPRYLTGFTYSLNTPLGKAFITINENGGNQPFEVFINTAKAGSDTA
                     AVSEAIGRLLSYVLRMSSPIEPVRRLDEVMRQLSGIGGGRPMGFGPNRVRSLPDGIAQ
                     VLEEYLRQRSERMAQGAPAAPSGNGHHHDLDEQRTAPLGGSLPLLKIGDLCPECGEAA
                     VINEEGCRKCYACGYSEC"
     gene            complement(1484..2104)
                     /locus_tag="PKL11_09585"
     CDS             complement(1484..2104)
                     /locus_tag="PKL11_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13895.1"
                     /translation="MKTVSCPAWFAWLVSLYPAEFRETFGPEMLAVFAAKLTAARSES
                     RKAMLAACLHELSELPGNLLTEHWLNLKKGDIPMVSTRRLVFTGLAIPLGVAVFLAII
                     NPNYMLNLFTGFLGWLAVISFLLLILLNGVLIWRGQRQDHPSPGWVVGLTLAALLCIF
                     LGPTLNRVSGMLPPIWTTVITIVLILVSLGCISVLATLLLKAQRTS"
     gene            complement(2101..2454)
                     /locus_tag="PKL11_09590"
     CDS             complement(2101..2454)
                     /locus_tag="PKL11_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ13896.1"
                     /translation="MDKSIQDALPLTETTLFILLSLSEEPRHGYAIMKSVLQLSKGRI
                     NLTAGTLYGAIKRLLGQDWIERIDDGDLVIDNPGLPRKAYQLTRRGRAILQAEVQRLQ
                     SVMQAVNLQVREEQL"
     gene            2877..3827
                     /gene="rsgA"
                     /locus_tag="PKL11_09595"
     CDS             2877..3827
                     /gene="rsgA"
                     /locus_tag="PKL11_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNZ13897.1"
                     /translation="MNATFDLIENNVGMITRKTTGAYAVQTRTETMECTLAGRLRHAG
                     LTDGLTVGDLVRLAPAGNSGWQIIETLPRRNQLSRRSAVPMPGAHAHEQVIAANVDQV
                     IPVFAAADPAPHWNMLDRYLVSAEAAEIPALVCITKLDLTQTAAGEPEADLLAVVEEY
                     RRIGYPVMMVSARTGAGLEALRQVLQGRVSVLLGKSGVGKSSLLNALEPGLGLRVSAV
                     SEYTGKGRHTTTHLEMFMLEYGGAIIDTPGVREFGLWDVAEDDLALFYPEMRPYVGRC
                     RFGLGCRHDEEPGCAIRKAVMDGQISPRRYQSYLRLQVEA"
     gene            3824..4291
                     /locus_tag="PKL11_09600"
     CDS             3824..4291
                     /locus_tag="PKL11_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HNZ13898.1"
                     /translation="MKAGAYSRRGERSGFGDFRCGHCHQYVSANPLLSGVQNRNHCPY
                     CLWSRHVDLFKAGDRLCACKALMQPVGLTLKRRHKRYMRPGQGELMIVHLCVECGGLS
                     INRVAADDDADALLAAFEAAQHHAAPLHRQMQASGIQPLSAAERPLVQMQFLI"
     gene            4439..>4535
                     /locus_tag="PKL11_09605"
     CDS             4439..>4535
                     /locus_tag="PKL11_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNZ13899.1"
                     /translation="MKRLILVAGNIGAGKTSLTERLGERMGWRTAY"
BASE COUNT          975 a         1233 c         1392 g          935 t
ORIGIN      
        1 atttgatgta ccatgtgggt gtgcggtacg gatcacctga ggggcaggaa tttgcagccc
       61 aggtgatgga atttgtgcgt taccaggcta tgcagaccag cgtggaactg gcaaaggttc
      121 gcggcgcctt cccggcgatt cagggcagca tctacgaccc cgatgatatg cagtgggaac
      181 caccgcagcc gctgatgccc tacgacgccg attggggccg cccagccctg gactgggagg
      241 cgctgacaga caacatccgg cagaatggca ttcgcaacgc ggcgcagacc actgtggcgc
      301 ctactggcac cattgccacc gtggcagggt gcgaaggtta tggctgcgaa ccggtctttg
      361 cgctggccta tatccgccat gtgaacgata acgggaaaga tttacaactc aattatgcca
      421 gcccgctgtt tgagcaggcg ctgattgaag ccgggctgga agccggtgca cgcgaggata
      481 ttgtggaggc ggtgatggct gccggaagct gccagaccgt ggagaccgtg ccggcgcggc
      541 tgcgcgatgt gttcgtggtc tcaggcgata tcagcgcgga agagcatgtg catatgcagg
      601 cggcgatgca ggcctttgtg gataacagcc tgtccaagac gatcaatttc cctgaaggcg
      661 ccaccaccga tgaggtgtcc aaagcttaca tgctggcctg ggaactgggc tgcaagggaa
      721 ttacggtgta tgtgaccggc tcgcgggaaa aggtggtgct ggagacccgg tccaccgccg
      781 ataagaaaca gcctgtggcc gaaaccccgc gccaactgga actgcctgct gcgcagccaa
      841 ccatctggca ggagagcaag aaaccccgcc cccgctactt gaccggcttt acctattcgc
      901 tcaacactcc gctgggcaag gccttcatca ccatcaacga gaacggcggc aaccaaccct
      961 ttgaggtgtt tatcaatacc gccaaggccg ggtcggatac ggcggcggta tctgaggcca
     1021 ttgggcggct gctgtcttat gttctgcgga tgtcttcgcc gattgaaccg gtgcggcggc
     1081 tcgatgaagt gatgcggcag ctttcgggca tcggcggcgg gcgaccgatg ggctttggtc
     1141 ccaaccgggt gcgctcactg cccgatggca ttgcgcaggt gctggaagaa tatctgcggc
     1201 agcgctctga acgcatggcg cagggtgcgc ccgcggcgcc gtctggcaac gggcatcacc
     1261 acgatctgga cgagcagcgc accgctccgt tgggcggcag cctgccgctg ctcaagattg
     1321 gcgacctgtg ccccgaatgc ggcgaggcgg ctgtcattaa tgaagaaggc tgccggaagt
     1381 gttatgcatg cgggtacagc gagtgttaat tttgtaaatt taggccaaaa cggctgatct
     1441 aaaataggtc agccgttttt tgatctggca atttactggc ggttcaactg gtgcgctgcg
     1501 ctttcaggag aagtgtcgcc aggacactga tgcaccccag gctgacgagg atcagtacaa
     1561 tggtgatgac ggtggtccaa atgggcggca gcatgccaga tacccggttg agagtggggc
     1621 ctaaaaagat gcacagtaac gcggcaaggg ttaacccaac aacccagccg ggcgacgggt
     1681 gatcttgccg ctgtccgcgc cagatgagga cgccgttcag caagatcaac agcaaaaagc
     1741 tgatgacagc cagccagccc aggaagcccg taaacaggtt caacatgtaa ttgggattga
     1801 taatcgccag gaaaaccgcc acccctaatg gaatggctag cccggtgaac accaaacggc
     1861 gggttgagac catgggaata tctccttttt tcaaatttag ccaatgttcg gtcagcagat
     1921 ttcccggcag ttcgctcagt tcgtgcaggc aggctgccag catggctttg cggctttcgc
     1981 tccgggctgc ggttaattta gcggcgaaga ccgccagcat ctccgggcca aatgtctctc
     2041 gaaattcagc cgggtaaagg cttaccagcc aggcaaacca ggccgggcag gaaacggttt
     2101 tcataactgc tcctcacgaa cctgcaaatt gaccgcctgc atcacagatt gcaggcgctg
     2161 aacttcagcc tgcaggattg cccgcccccg gcgagtcaac tggtaggctt tgcgcggcaa
     2221 gccagggtta tcgataacaa gatcaccatc atcaatgcgc tcgatccaat cctgtcccag
     2281 cagacgtttt atcgccccgt aaagggttcc agcggtcaaa ttaatccggc ctttgctgag
     2341 ctgcaaaacc gatttcataa tggcatagcc atgtcggggt tcttctgaca ggctcaacaa
     2401 gatgaaaagg gttgtttcgg tgaggggcag agcgtcttga attgatttat ccatctggat
     2461 tgaattcctt ttcgttcaat atatcgcgca tctatatatc gttattcgat attattttag
     2521 cacgcccagg ccaaaaatca agccccaatt ttgcagaatt cgaagctcga aacaaaaatg
     2581 aatcattctt ggtaaattcc ttgacagttc cggcaaggta gtgatacact ttttgccgtt
     2641 cagattttat cttgcgaaag gagcgcacag ttatatggct aaagtatcgg tattggtccg
     2701 agttcaagat gcatgtgcgc gcctggagat tgccgggtaa aatctgacgg ttaacgttca
     2761 gatccagcca cgggcgcgtc catgccccgt ggcttttgtt ttaatcacat cgaaacacat
     2821 caagccagcc acgggttacc tgcggctggc tttttctcat cttaggaaac tgaacgttga
     2881 acgctacttt tgacttaatt gaaaacaacg tgggtatgat tacccgcaag acaaccggcg
     2941 cttacgccgt tcaaacccgc acagaaacca tggaatgtac cctggcaggg cgcctgcgcc
     3001 atgctggatt gacggatgga ttgactgtgg gcgacctggt gcggctggca cctgccggca
     3061 acagcggctg gcagatcatc gagacgctgc cccggcgcaa ccagctttcg cggcgctcag
     3121 cggtgcccat gccgggtgcc cacgcacacg aacaggtgat tgccgccaat gtggatcagg
     3181 tgatcccggt gtttgcggca gccgacccgg cgccgcactg gaatatgctg gaccgttacc
     3241 tggtctcggc tgaagccgca gagatcccgg cgctggtctg catcaccaag ctggacctga
     3301 cgcagacagc cgcaggtgag cctgaagctg atctgctggc tgtggtggag gagtaccggc
     3361 gcattggcta cccggtgatg atggtgagcg cgcgcactgg cgccgggctg gaagccctgc
     3421 ggcaggtgct gcaagggcgc gtgtcggtac tactggggaa gtcgggcgtg ggcaagagca
     3481 gcctgttgaa tgcgctggaa cccgggttgg ggctgcgcgt cagcgcggtg agcgaatata
     3541 cgggcaaggg gcggcacacc accacccacc tggaaatgtt tatgcttgag tatggcggag
     3601 cgatcattga tacgcccggc gtgcgcgagt ttgggctgtg ggacgtggcc gaagacgacc
     3661 tggcactgtt ttacccggag atgcgcccgt atgtggggcg ctgccggttt gggctgggct
     3721 gccgccacga tgaagaaccc ggctgcgcca tccgcaaggc ggtgatggac ggacagatca
     3781 gcccgcggcg gtatcagagt tacctgcggt tacaggtgga agcatgaaag ccggcgctta
     3841 ttcacgccgc ggggagcgca gcggatttgg tgattttcgc tgcggtcact gccatcagta
     3901 tgtttcggct aacccgctgc tgtcgggggt gcagaaccgc aatcactgcc cctactgcct
     3961 gtggtcacga catgtggatc tcttcaaagc gggcgaccgg ctgtgcgcct gcaaggcgct
     4021 gatgcagccg gtggggttaa ccctcaaacg ccgccataag cggtacatgc ggcccgggca
     4081 aggcgagctg atgattgtgc atctctgcgt cgaatgcggc gggttgtcca tcaatcgggt
     4141 ggcagcagat gatgatgccg acgcgctgct ggccgcgttt gaagcggcgc agcaccatgc
     4201 ggcgcccctg caccgtcaga tgcaggcttc gggcatccag ccgctttcgg cagccgagcg
     4261 cccgctggtg cagatgcagt tcttaatcta gacaagggga tttggccgga cggtcaaatc
     4321 ccctctactc gccatttaag tggaaccagg atctatcaaa acctgatttc gataacaagg
     4381 cgtcggattt acctgtcttt cagaactgac cattcagcct taatgctata atcacatcat
     4441 gaaacgcttg attctggtgg caggaaatat tggcgccggc aagacctcgc tgaccgagcg
     4501 gctgggtgag cggatgggct ggcgcacggc ttacg
//
LOCUS       DAOPVS010000154         4524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_19251_length_4524_cov_60.140300, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000154 DAOPVS010000000
VERSION     DAOPVS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19251_length_4524_cov_60.140300"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="uppP"
                     /locus_tag="PKL11_09610"
     CDS             complement(<1..785)
                     /gene="uppP"
                     /locus_tag="PKL11_09610"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNZ13900.1"
                     /translation="MTILQAVILGIVQGLTEFLPVSSSAHLVLVPFLFNWQIPADQIF
                     PFDVLVQLGTVVAVIAFFWKDLWTIVRAWVSGLIKRQPFADPQSRLGWYLIIATIPAG
                     LAGVLFKDLVEQAFHSPTATALFLLVTAALLTLAEWLGKRTRSLEAMNWKDALVMGLM
                     QVVSLLPGVSRSGSTISGGMFRGLDRASAARFSFLMSVPVMLGAGLIGFKDLLDVPNL
                     AGFLPVMVIGFVAAAVVGYLSIAWLLRFLTRRSFYGFAIYCVA"
     gene            complement(782..1906)
                     /gene="tgt"
                     /locus_tag="PKL11_09615"
     CDS             complement(782..1906)
                     /gene="tgt"
                     /locus_tag="PKL11_09615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNZ13901.1"
                     /translation="MGPVNTPAFFYELLAEDGGARAGIFHTPHGDIPTPVFAPVGTQA
                     TVKAVTPDQLEALGASLVLANTYHLYLRPGAPLVAEMGGLHQFMQWPHPMLTDSGGFQ
                     VFSLADMRKIDADGVTFKSHIDGSTHRLTPEKAVQIQEQLGADIIMAFDECAPPYDRA
                     YNERALERTHAWALRCLQARTRADQALFGIVQGGVFPDLRQRSAEFIATQDFPGYAIG
                     GLSVGETKPEMHAMIEVVNAILPTDRPRYLMGVGAPEDLVNGVMRGIDIFDCVLPTRL
                     ARHQAAMTLDGRINLMNAANARDPRPIDAACGCYTCQHFTRAYLRHLIVAKEILASTL
                     ISIHNLYTLEELMRQMRTAILEHRFAAFAAATLSRLEAKQ"
     assembly_gap    2071..2170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2171..2914
                     /gene="alr"
                     /locus_tag="PKL11_09620"
     CDS             <2171..2914
                     /gene="alr"
                     /locus_tag="PKL11_09620"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNZ13902.1"
                     /translation="VKIDSGMGRLGKFPEDGVEFIRWLKTLPGAEVEGLFTHFARSDE
                     PAHPFTNEQIACFTTLVKALEENNLRPRVIHAANSAAALYFPAARFDMVRSGIALCGL
                     HPDEKDARLPDDFETALTWKARLTSVKILPPGHCVGYMHRYVTTSTERIGAISAGYAD
                     GLPRRVGNPVIVGGKRVKVVGGTGMDQCMVPLDGIPDAQVGDEVVLIGRQGEARLTAE
                     EVGAEWGTNNYDVVCGLQARLPRVYFNRE"
     gene            2914..>4524
                     /gene="recJ"
                     /locus_tag="PKL11_09625"
     CDS             2914..>4524
                     /gene="recJ"
                     /locus_tag="PKL11_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNZ13903.1"
                     /translation="MNTITSKRWLIPDPLPSEVDEALVDYPPTIRQILYNRGCQTAEE
                     AEAYLAGKVEEGDPFQLTDMEAAVERILRALDADESIAIYGDYDVDGVTATTLLVEVL
                     REMGGQAEAYIPNRFDEGYGLNNEALDELKARGFGLVITVDCGIRSPREAEHAREIGL
                     DIIITDHHHPHDEIPAATAVICQRKPGEAYPEVNLAGVGLAYKVAQGLLARRPLPDRN
                     ATDWLDLVALGTVADLVPLTGENRALVRGGLRLLRMGGRQGALSLALAAGLTVSKITA
                     SDIGFSLGPRLNAAGRLESALAAYDLLIARDVEVAGLLAQQLDDKNRKRQQMTVEMQT
                     KAAELAAQEESDYLIFAVHPEFNSGVVGLAAARLVEQYYRPAVVGQIGEQFTRASCRS
                     IPEFHITDALDECSDLMVRHGGHAMAAGFTISNENLPELQARLQEIARRKLSEIELRP
                     ALRADVVIPLSELHPRLLSRLDQLQPTGLGNPDAVFVSRNVRVRYFKAVGEEGRHLRM
                     VVTDGKIDFSAIAFRQGHWANQMPERVDL"
BASE COUNT          928 a         1215 c         1518 g          763 t
ORIGIN      
        1 aaagccacgc agtagatggc gaagccgtaa aacgagcggc gggttaaaaa gcgcagcagc
       61 caggcgatgg acaggtaacc gaccactgcc gccgccacga aaccgatcac catcaccggg
      121 agaaagccgg cgaggttggg cacgtccagc agatctttga agccaatcag accggcgccc
      181 agcatcaccg gcactgacat caggaaggag aagcgcgccg ctgaggcccg atcaaggccg
      241 cggaacatac cgcccgagat ggtggagccg gagcgggaga cgcccggcag cagggagacc
      301 acctgcatca accccatgac cagggcatct ttccagttca tagcctccag gctgcgggtg
      361 cgcttgccca gccactcggc cagggtcagc aaagcggcag tgaccagcaa aaacagcgca
      421 gtagctgtgg gggaatggaa ggcctgctcg accagatctt tgaacagaac cccggccagg
      481 cccgccggaa tggtagcaat aatcagatac cagcccaggc gggattgggg atcggcaaag
      541 ggctggcgct taatcagtcc agatacccag gcccggacaa tggtccagag gtctttccag
      601 aaaaaggcga tgacagccac cacggtgccg agttgaacca ggacatcaaa ggggaagatc
      661 tgatccgcgg ggatctgcca gttgaacagg aagggcacca gcaccagatg ggcggagctg
      721 gacacgggta aaaattcagt gagaccctgg acgatgccca ggataacagc ctgcaaaatg
      781 gtcattgttt tgcctccagg cgggatagtg tggcagccgc aaacgcggca aagcgatgct
      841 caaggatggc ggtacgcatc tggcgcatca gttcttcgag ggtgtagagg ttatgaatgg
      901 agatgagggt ggacgccaga atttctttag ccacaatcag gtggcgcagg taggcgcggg
      961 tgaagtgctg gcaggtgtaa cagccgcagg cggcatcaat gggacgggga tcacgggcgt
     1021 tggcggcatt cattaaatta atgcgcccat ccagggtcat ggcggcttga tggcgcgcca
     1081 ggcgggtggg cagcacacaa tcaaagatat caatgccgcg catcacgccg ttgaccagat
     1141 cttcgggcgc gccgacaccc atcaggtagc gcgggcggtc cgtggggagg atggcgttga
     1201 cgacctcgat catggcgtgc atttcgggct tggtctcgcc aaccgagagg ccgccgatgg
     1261 cgtagccggg gaaatcctgg gtggcgataa attcagccga gcgctggcgc agatcgggga
     1321 aaaccccgcc ctggacgatg ccgaacaaag cctgatcagc ccgggtgcgg gcctgcaaac
     1381 agcgcagcgc ccaggcgtgg gtgcgctcga gggcgcgctc gttataggcc cggtcgtagg
     1441 gtggggcgca ctcgtcaaag gccatgatga tatcggcgcc aagctgctcc tgaatctgga
     1501 cggccttttc gggcgtcagg cggtgggtgg agccgtcaat atggctcttg aaggtcacgc
     1561 catcggcatc aattttgcgc atgtcggcca gggaaaaaac ctgaaagccg ccggaatcgg
     1621 tcagcatggg gtgcggccac tgcataaact gatgcaagcc gcccatctca gcgaccaacg
     1681 gcgcaccggg gcgcaggtag agatgatagg tgtttgccag caccagcgaa gcgcccagcg
     1741 cctccagttg atcgggcgtg acggctttga cggtggcctg agtgcccacc ggcgcaaaga
     1801 ccggcgtagg gatatccccg tgcggggtgt ggaagatgcc ggcgcgggcg ccgccatctt
     1861 cggccagaag ttcatagaaa aaagcggggg tgttcacagg tcccattata ccaatcttct
     1921 ccttgtcggt ttgacggcgt gaaagtatac tagggcgatg aacatcgcaa actattcaac
     1981 ctggctggaa attgacctgg gggcggttcg caataatgtg cgaaggatga aagcaatcac
     2041 tggccgcggc gtgatcgcgg tcattaaagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn gtcaaaattg acagcggcat ggggcggctg ggtaagttcc cggaagacgg
     2221 ggtggagttt atccgctggt tgaagacgct gccgggcgcc gaggttgagg ggctgttcac
     2281 gcattttgcc cgctcggatg agcctgcgca cccctttacc aatgagcaaa ttgcctgctt
     2341 caccacgctg gtgaaggcgc tggaagaaaa caacctgcgc ccgcgggtca ttcatgccgc
     2401 caattcagcg gcggcgttgt attttccggc ggcgcggttt gatatggtgc gcagcgggat
     2461 tgcgctgtgc gggctgcacc cggatgagaa ggatgcccgg ctgccggatg actttgaaac
     2521 ggcgctgacc tggaaggccc ggctgacctc ggtcaaaatt ttgccgccgg ggcactgtgt
     2581 gggctatatg caccgctatg tgaccaccag caccgagcgg attggggcca tctcggcggg
     2641 atatgccgat ggtctgccgc ggcgggtggg caacccggtg attgtcggcg ggaagcgcgt
     2701 caaagtggtg gggggcacgg gcatggatca atgcatggtg ccgctggatg gcattcccga
     2761 tgcccaggtg ggcgacgaag tggtgctgat tgggcggcag ggcgaggccc ggctgaccgc
     2821 cgaagaagtg ggcgccgagt ggggcaccaa taactacgat gtggtctgcg ggctgcaagc
     2881 ccggctgccg cgggtatatt tcaatcgaga ataatgaata cgattacatc caaacgctgg
     2941 cttatccctg acccgctgcc atctgaagtt gatgaagcgc tggtagatta ccccccaacc
     3001 atccggcaga tcctgtataa ccggggctgt cagaccgctg aagaggctga agcctacctg
     3061 gcgggaaaag ttgaggaggg cgacccattc caactgacgg atatggaagc cgcggtagaa
     3121 cggattctgc gggcgctgga tgcggatgaa tccatcgcca tttatggtga ctatgatgtg
     3181 gatggcgtga cggccaccac actgctggtg gaagtgctgc gggagatggg ggggcaggct
     3241 gaagcgtata ttcccaaccg gtttgatgaa ggctacgggc tgaacaatga ggcgctggat
     3301 gaactgaagg cgcgcggctt tgggctggtc atcacggtgg actgcggcat ccgctcgccg
     3361 cgggaggccg agcacgcccg cgagatcggg ctggacatca tcatcaccga ccaccaccac
     3421 ccgcacgatg aaattccggc agcaaccgcc gtaatttgcc agcgcaaacc gggcgaagcc
     3481 taccccgaag tcaacctggc gggggtgggg ctggcctaca aggtggccca ggggctgctg
     3541 gcccgccgcc cgctgccgga ccgcaacgcc accgactggc tggatctggt ggcgctgggc
     3601 accgtggccg acctggtgcc gctgacagga gaaaaccgcg ccctggtgcg cggcgggctg
     3661 cggctgctgc ggatgggggg acggcaaggg gcgctttcgc tggcgctggc tgccgggctg
     3721 acagtttcaa aaatcacagc gtcggatatc gggttcagcc tggggccgcg gctcaacgct
     3781 gccgggcggc tggaatcggc gctggcagcc tatgacctgc tgattgcccg cgatgtggaa
     3841 gtggccgggc tgctggccca gcagttggac gataaaaacc gcaagcgcca gcagatgacc
     3901 gtggagatgc aaaccaaggc cgctgaactg gcggctcagg aagaatcgga ttacttgatt
     3961 tttgccgtgc atccggaatt caattccggc gtggtggggc tggccgcggc gcggctggtg
     4021 gagcagtatt accgcccggc ggtggtgggg cagattggcg agcagttcac ccgtgcatcg
     4081 tgccgcagca ttcccgagtt tcatattacc gacgctttgg acgagtgctc ggatttgatg
     4141 gtgcgccacg gcggacatgc tatggcagcc gggttcacca tcagcaatga aaacctgccc
     4201 gaactgcaag cccgcctgca ggagattgcc cggcgcaagc tgagcgaaat tgaactgcgc
     4261 ccggcgctgc gggcggatgt ggttatcccg ctgtctgaac tgcatccccg gctgctcagc
     4321 cggctggatc aactgcagcc gactggcctg ggcaacccgg atgcggtgtt cgtctcgcgc
     4381 aatgtgcggg tgcgttactt caaagcggtg ggggaagagg gccggcacct gcgcatggtg
     4441 gtgacggatg gcaagattga cttcagtgcc attgccttcc ggcaggggca ctgggccaac
     4501 cagatgcccg agcgcgtgga tttg
//
LOCUS       DAOPVS010000155         4489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_19449_length_4489_cov_38.927154, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000155 DAOPVS010000000
VERSION     DAOPVS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19449_length_4489_cov_38.927154"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKL11_09630"
     CDS             <1..266
                     /locus_tag="PKL11_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNZ13904.1"
                     /translation="IMQIARKHSIVRVYVFGSVARGEASPQSDVDFLVEMRAGASLFG
                     VAGFGYEVEKLLGIPVDVVPMSALACITDQEFVRHIQSEAVAI"
     gene            263..586
                     /locus_tag="PKL11_09635"
     CDS             263..586
                     /locus_tag="PKL11_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNZ13905.1"
                     /translation="MKDTAQLLQDILDSITTIETYAVSSYESFLEDEKTQDAVMFNLI
                     IIGEAANRIAGEFQESHPEIPWSSMIGTRNVIVHGYDQVKLQIVWDIIHRDLAPLKEK
                     VSKAI"
     gene            661..1311
                     /locus_tag="PKL11_09640"
     CDS             661..1311
                     /locus_tag="PKL11_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13906.1"
                     /translation="MSILNQLSSQVGDRTEYANRRVAALCLQDPNLIDAIAAGLEGSN
                     AALMGDCAEVLTLVAEQHPEWAAPHAEALAARLSHKNTRVRWEAMHALALIAMRTPEV
                     IAPRLSTLGELIQQDASVIVRDYAVDAVGHYAAAGPQAAEAAYPWLVSALSAWNGKHA
                     GHALTGLIHVARHLPDLHPKLAAIADDYARSERAVIRKAAKGLQKALKSLPGSGGS"
     gene            complement(1308..2000)
                     /locus_tag="PKL11_09645"
     CDS             complement(1308..2000)
                     /locus_tag="PKL11_09645"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNZ13907.1"
                     /translation="MTTREFFKHWLALTLGLGGVLAGYNAITLGWGGVLLGLGLALIF
                     ALVWVLLERRAYTRPTRPLAYEALLLPVINTRFEFPVREIDTWATLGAVLIVTVSNTT
                     WSEPLSRLQLLYMGCLLAAAVLLRLVEGKHTRLNILPTGVEVQALFYTLTIPWSAVER
                     IEQRKYRWILSYQSCEMKASPLVTAWLKFKGFDRRLFLNKFALDYPKSNLARAIYQRG
                     EAKIKHFTTEKG"
     gene            complement(2082..4031)
                     /locus_tag="PKL11_09650"
     CDS             complement(2082..4031)
                     /locus_tag="PKL11_09650"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNZ13908.1"
                     /translation="MNRAYYDAPIEVFRTENPLTILGALAEHHDFDLEDRQRLAWQQE
                     ISLLQEVLAGFNGHICFEFSIPRMGKRADVVLLMDGIVFVLEFKVGETNYPAQAIEQV
                     MDYALDLKNFHEPSHNRPIVPFLISTDARPIPNQMMPDADQVYKPICANTLTVTDLLH
                     ACMGSIQAEPIDPSVWLAGQYKPTPTIIEAAQALYRGHNVREISRSDSGAINLSKTSD
                     AIDDIIEYAQKHHQKAICFITGVPGAGKTLAGLNIANRRLHAGEDEHAVFLSGNGPLV
                     IVLREALTRDDIAKGQKRKDAHRKADMFIQNIHHFRDEYLGNANAPLEQVVIFDEAQR
                     AWGEGMLANFMKRKRGQPNFTMSEPELLIEVMDRHMDWAVIICLVGGGQEINTGEAGL
                     RQWFQVLQADFPNWHVYISKQITDSEYLGQESPINLLPQQQLTVNDDLHLAVSVRSFR
                     SEKVSALIKSILDGDLKTSQELCRFVTPHYPIVLTRDLESARNWVRQQARGSERTGLL
                     ASSGARRLRPEGIFVDAEIEVAHWFLNGREDVRSSYALEGVATEFDIQGLELDWTVVG
                     WDGDLRYLKGRWEYRAFKGTRWQAVNDLNRQLYLKNSYRVLLTRARQGMVIFVPKGDK
                     TDITRLPEFYDGTFEYLRQVGLTVI"
     gene            complement(4024..4236)
                     /locus_tag="PKL11_09655"
     CDS             complement(4024..4236)
                     /locus_tag="PKL11_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ13909.1"
                     /translation="MTAEILLHLGKRIRSIREKAGISQEALGIKSGLHRTYVGAIERG
                     DRNPSVLSLKKLADALGVKLGDFFDE"
BASE COUNT         1058 a         1187 c         1190 g         1054 t
ORIGIN      
        1 agatcatgca aattgcccga aaacacagca ttgtgcgggt ctatgttttt gggtcagtgg
       61 cgcgtggcga agcaagccca cagagcgatg tggattttct ggtagaaatg cgcgcgggtg
      121 cgtcgctgtt tggcgttgct ggatttggtt atgaggttga aaaattactg ggaatcccgg
      181 tggacgtggt gccgatgtct gctttagcct gtatcactga ccaggagttt gtgcgccata
      241 ttcaaagcga agctgtagca atatgaaaga tactgctcaa ctcttgcagg atattttgga
      301 ctctattaca acaattgaga cctatgctgt ttcatcttat gaaagttttt tggaagatga
      361 aaaaacccag gatgcggtta tgtttaattt gatcatcatt ggcgaagcag ccaatcgaat
      421 tgccggtgaa tttcaagaaa gccatccaga aattccatgg tcttcaatga ttggaaccag
      481 aaacgtgatt gttcacggtt atgatcaggt taagttgcaa attgtatggg acattattca
      541 tagagattta gcacctctaa aagaaaaggt atccaaagca atctgaccct ggaaaaatca
      601 ggctcgattt gaggacaagc gaataagcaa gcaatgcgag acggtggagg tacaaccgcc
      661 atgtccattc tcaaccagtt atcttcgcag gtgggtgacc gaaccgagta tgccaaccgc
      721 cgggtggcgg cgctgtgcct gcaagacccc aatttgattg atgcgattgc tgccgggctt
      781 gaaggctcca atgcagccct gatgggtgac tgcgccgagg tgctgacgct ggtggctgag
      841 cagcatcccg aatgggctgc gccccatgcc gaagccctgg ccgcgcggtt gagccataaa
      901 aacacgcggg tgcgctggga agccatgcac gcgctggcac tgattgccat gcggacgccg
      961 gaggtgattg caccgcggct gtccaccctg ggggaactca tccagcagga tgcgagcgtg
     1021 attgtgcgcg attatgcggt tgatgcggtg ggccattacg ccgccgccgg gccgcaggct
     1081 gccgaggcgg cttacccctg gctggtgagc gcactttcgg cctggaatgg caaacatgcc
     1141 ggccacgcgc tgaccgggct gatccatgtg gccaggcacc tgccagacct gcacccgaag
     1201 cttgcggcca tagccgacga ttacgcccgc agcgagcgcg ccgtgatccg caaggcggcc
     1261 aaaggtttgc agaaggcgct caagagtctg ccggggtcag gcggctccta gcccttttcg
     1321 gtggtgaagt gcttgatttt tgcctcaccg cgctggtaga tggcccgcgc cagattgctt
     1381 ttgggataat ccagcgcaaa cttgttcagg aagaggcgcc ggtcaaaccc cttgaatttg
     1441 agccacgcgg tcaccagcgg gctggctttc atctcgcagg actggtagga caggatccag
     1501 cggtacttgc gctgttcaat ccgctcgact gcgctccagg ggatggtgag ggtgtagaac
     1561 agtgcctgca cctccacccc ggtgggcagg atattcaggc gggtatgttt gccttctacc
     1621 aggcgcagca gcactgccgc cgccagcagg cagcccatgt agagcagctg cagccggctg
     1681 agcggctcag accaggtggt gtttgagacg gtgacaatta acaccgcccc cagggtggcc
     1741 caggtatcaa tctctctgac ggggaactcg aaccgggtat tgatgaccgg cagcagcagc
     1801 gcctcatacg ctagtggacg ggtggggcgg gtgtaggcgc gccgctccaa caaaacccat
     1861 accagggcaa agatcagtgc aagccccagg ccgagcagca cgccccccca gcccagcgta
     1921 atggcgttat agcccgccag cacgcccccc aatcccagcg tcagcgccag ccagtgcttg
     1981 aaaaattctc gtgtggtcat gtgatttccg ggtgaagatc aggtttggat gcttgattat
     2041 agcttcaatt gggagcaggg ggggattttg gcggcgcaat cctaaatgac agttagccca
     2101 acctgccgca agtattcaaa cgtaccatca taaaactcgg gcaggcgcgt gatatcggtt
     2161 ttatcgccct ttggaacgaa aatcaccatg ccttgacggg cacgtgtgag cagtactcga
     2221 taggaatttt ttaagtaaag ttgtcggttg aggtcgttga ctgcctgcca ccgggtgccc
     2281 ttgaatgccc ggtattccca gcggcctttc aaatatctca aatcaccatc ccaacccacc
     2341 acagtccaat caagttccag cccttgaatg tcaaattcag ttgcaacgcc ttccagcgcg
     2401 taagaggagc gcacatcttc ccgtccattc aagaaccaat gtgccacttc gatttcggca
     2461 tcaacaaaaa taccttcggg gcgcaaccga cgcgcccctg agctagccaa tagacccgtg
     2521 cgctcactgc cccgagcctg ctggcgtacc cagttgcggg cagattcaag gtcgcgtgtc
     2581 aagacgattg gatagtgggg cgtgacaaag cggcatagtt cctgagatgt ttttagatca
     2641 ccatctaaaa tggatttgat tagggcagat actttttcag accgaaatga acgtacggaa
     2701 accgccagat gaaggtcatc attaacggtt aattgttgtt ggggcagtaa attgatcggt
     2761 gattcttgtc cgaggtattc agaatcggtg atttgttttg agatgtaaac gtgccaattt
     2821 gggaagtctg cttgcaagac ctgaaaccac tgcctcaaac ctgcttcgcc agtgttgatt
     2881 tcctgcccac caccaaccag acagatgatc acagcccaat ccatgtgccg gtccatcacc
     2941 tcgattaaca gctcgggttc agacatggtg aagttgggtt gaccgcgttt acgcttcata
     3001 aaattagcca acatgccctc accccaagcg cgctgagcct catcaaaaat aaccacctgt
     3061 tctaacgggg cgttggcatt tcccaaatat tcatcgcgaa agtgatgaat attttgaata
     3121 aacatatctg ctttacggtg agcatctttt cgcttctgcc ccttggcaat atcatcacgg
     3181 gtcaaggctt cgcgcaagac aataaccaat ggaccattgc cggacaaaaa gacagcgtgc
     3241 tcgtcttccc cagcgtgtag ccgccgattg gcaatattca gccccgccag ggtttttcca
     3301 gcccctggca ccccagtaat aaaacagatg gccttctgat gatgtttctg ggcatactca
     3361 atgatgtcat caatcgcatc cgatgtttta ctcaggttaa tcgctccact atccgagcgg
     3421 gagatttcac gtacattatg cccccggtat aatgcctggg cagcttcaat aatcgttggc
     3481 gttggcttat attgccctgc cagccacacc gacggatcaa tgggttcagc ctggattgat
     3541 cccatgcaag cgtgtagtaa atccgtcacc gtgagcgtat tggcgcaaat cggtttatag
     3601 acctgatctg catctggcat catttgattg ggaatgggtc gggcatccgt ggaaatcaga
     3661 aacggcacaa tggggcggtt atgactcggc tcgtggaaat ttttcaaatc cagggcgtaa
     3721 tccatcactt gttcaatggc ttgtgccggg taattggttt cacccacctt gaattctaaa
     3781 acaaatacga tgccatccat aagcagaaca acatctgcgc gcttgcccat ccgtggaatt
     3841 gaaaattcaa agcaaatgtg tccgttgaaa cctgccaaga cttcttgtaa caggctgatc
     3901 tcctgttgcc aggcaagcct ctgccgatcc tccagatcaa agtcgtggtg ctcagccagt
     3961 gcccccaaga ttgttagggg attctctgtc cgaaagacct caataggagc gtcataatag
     4021 gctctattca tcaaagaaat cccccagctt tacacccaaa gcatcggcaa gtttcttcag
     4081 gctcaagact gaggggttgc ggtctccgcg ttcaattgcc cccacataag tgcgatgcaa
     4141 gcccgatttt attcccagtg cttcttgcga aatcccggct ttttctctaa tggaacgaat
     4201 gcgttttcct aagtggagaa ggatttctgc ggtcattttg ggctccttgg gttgaccaac
     4261 atccataaga ccaggatacc cgcgatgcta ctggtgggct acagactata agtagcaatt
     4321 aagccattca gaccaggaac gaccttatgc ccccttgcct tccccgctta cccccatcca
     4381 cggggtttga caaattgccc cggtgcgcat atactttgca gcgtcaatct tttaagttgc
     4441 cttcggggca gggtgcggga catgcgtccc aattcccgat cggtggtca
//
LOCUS       DAOPVS010000156         4487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_19457_length_4487_cov_62.234883, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000156 DAOPVS010000000
VERSION     DAOPVS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19457_length_4487_cov_62.234883"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2573)
                     /locus_tag="PKL11_09660"
     CDS             complement(<1..2573)
                     /locus_tag="PKL11_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNZ13910.1"
                     /translation="MEAKIWHTLKQDEINAALETSPQGLTSAEAAKRLAEIGPNEIQA
                     AKRISAFEILLAQFKNILILILLGATIISLFLGHGIESVVIAVIVLFAVALGFVQEYR
                     AERAIEALREMAAPTATVLRDGDDTEIPARELVPGDVILLNTGDRVPADARLLEAVNL
                     QVEEAALTGESMPIEKHTQPLDNSELSVGDRKNMVYAGTAVTYGRGRALVAATGMQTE
                     FGKIAQMLQTVETGKTPLQHNLDKLGAVLARAAFVVVALIVAVGLVRGQPLVEMLIFG
                     IALAVAVVPEALPAVVTISLAIGVQKMVKRNALIRRLPAVETLGSTSVICSDKTGTLT
                     KDEMTARQLYCAGEVIRVSGAGYAPEGEFTTPDGAPAQMTPALREMLMAGVLASDTRL
                     IRTPGEGWDIKGDPTEGALIVAAAKAGLQKEALDAAHPRMGEIPFSSETKRMTTLHQT
                     ENGLTAYAKGAPEVILASCDFAATAEGSQPLDAAGREKILQQAQSMASQALRVLGIAA
                     KPQAELATAEKGMTFLGLVGMIDPPRPEAKAAIAVCADAGIRPVMITGDHPVTAQAVA
                     RELGLLQSGGRVVTGAELEEMSDEQLLKDVEEISVYARVSPAHKLRVVSAWQERGHIA
                     AMTGDGVNDAPALKKADIGIAMGITGTDVTKEAAAMTLTDDNFASIVAAVEEGRGVFG
                     NIKKYLMYLLSSNIGEIGLMAGSALLGLPLPLTAVQILYVNLATDGLPALALSVDPPE
                     KDLMRRKPRNPRTGIFTRPVVTLMALGGLWSTLINLSLFSWAINSGRTLEQAMTMTFV
                     SLVLIQFFKAYNFRSDRYSVLDKPFANKWLNLSIVWEIIMLLLIVYLPILHEPFNTYA
                     LP"
     gene            complement(2650..3309)
                     /locus_tag="PKL11_09665"
     CDS             complement(2650..3309)
                     /locus_tag="PKL11_09665"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNZ13911.1"
                     /translation="MIDQIEIWILGLLQTLYNAWGWLGVAALLVFENATGITPSEVIL
                     GFAGWMLIAEHELPVSMIFLGGLYSAIGSVIGASITYWVARLGGRPVVDRFARWVRIA
                     PEHIARAEQLFQRWGTGLVLIGRMIPAIRTLISIPAGLARMPFGTFVTTTFIGAYAWC
                     TLLIGAGYLLGHEWELISLYLKQALPYLLVGGAVALGIYFWWTRRRAAPALVLAPGED
                     E"
     gene            complement(3306..3719)
                     /locus_tag="PKL11_09670"
     CDS             complement(3306..3719)
                     /locus_tag="PKL11_09670"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HNZ13912.1"
                     /translation="MGKKLWIIGGTICLGLGIIGIILPVLPTTPFLLLAAFCYGRGSE
                     GFYQWLVYRSPFGSYIRDYRDGKGIPLRQKLITLTLLWLTIGTAILFIVDSEWLKALL
                     FVIAVGVTTHLSRVKNRLPEPAALTTTPPQQGSVE"
     gene            complement(4006..>4487)
                     /locus_tag="PKL11_09675"
     CDS             complement(4006..>4487)
                     /locus_tag="PKL11_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13913.1"
                     /translation="GAGEAATASGKVWVAQPGGQVIKYTLTLESDTVFGAGITGQQSW
                     EYTLRDPGEGAMQLPESCPLPLRGIPVPDGATGLVELPAEVRFQTALDAGTLAAFYTD
                     QLGALGYLPQGEGLETANGPLWAYIQQGEGVQTMLVITATPAEGGLSVRVVRVEIKP"
BASE COUNT          941 a         1459 c         1193 g          894 t
ORIGIN      
        1 ggcagggcgt aggtgttgaa gggttcatgc aggatcggca gatagacgat cagcagcagc
       61 atgataatct cccacacgat ggacaggttg agccacttgt tggcaaaggg cttgtccagc
      121 accgaatagc gatccgaccg gaagttatag gccttgaaga actggatcaa taccagcgag
      181 acaaatgtca tcgtcatcgc ctgttccagt gtacgcccgg agttgatcgc ccaactgaac
      241 aggctcagat tgataagggt ggaccacagc ccgcccagcg ccatcagggt caccaccggg
      301 cgggtgaaga tccccgtgcg cgggttgcgg ggtttgcggc gcatcaaatc cttctcgggc
      361 gggtcaactg aaagcgccag cgctggcagg ccatccgtcg ccaggttgac gtagagaatc
      421 tgcaccgcgg tcagcggcag gggcagcccc agcagcgccg agcccgccat caacccaatc
      481 tcgccgatgt ttgacgagag caggtacatc aggtattttt tgatattgcc aaataccccg
      541 cgcccctctt ccaccgcggc cacaatcgag gcgaagttgt catccgtcag cgtcatggcg
      601 gcggcttctt tggtcacatc ggtgccggtg atgcccatgg caatgccaat atctgctttc
      661 ttgagcgccg gcgcatcgtt cacgccgtcg ccggtcatgg cggcaatgtg gccgcgttcc
      721 tgccaggcgc tcaccacccg cagcttgtgt gcaggtgata cccgggcata cacgctgatc
      781 tcttccacgt ccttcaagag ctgctcgtca gacatttcct ccagctcggc gccggtcacc
      841 acgcgcccgc cgctttgcag cagccccagt tcgcgcgcca cagcctgagc ggtcaccggg
      901 tggtcgccgg taatcatcac cgggcgaata cccgcatccg cgcagaccgc aatggctgct
      961 ttggcttctg gccgcggcgg gtcaatcatc cccaccagcc ccaaaaaggt cattcccttc
     1021 tcagcagtcg ccagttcagc ctgcggcttg gcggcaatgc ccagcacccg cagggcctgg
     1081 cttgccatac tctgtgcctg ctgcaaaatc ttctcccgcc cggctgcatc cagcggctgg
     1141 ctaccttcag ccgtggctgc aaaatcgcag cttgccaaaa tcacctcagg cgcgcccttg
     1201 gcataagcgg tcagcccgtt ttcggtctga tgcagggtgg tcattcgctt ggtctcagac
     1261 gagaagggga tttcacccat tcggggatgt gccgcatcca gggcctcttt ttgcagtccg
     1321 gctttagctg cagccacaat caacgcaccc tcggtcgggt cgcctttgat gtcccagccc
     1381 tcgccgggtg ttcggatcaa ccgggtgtct gaagccagca caccagccat cagcatctcg
     1441 cgcagcgccg gggtcatctg cgccggggcg ccgtccgggg ttgtaaattc gccctcgggg
     1501 gcataccccg cgcccgaaac acggatgact tctcccgcac agtacaactg ccgggccgtc
     1561 atctcatctt tggtcagtgt gccggtcttg tccgagcaga tcaccgaggt gctgcccagg
     1621 gtttccaccg ccggcaggcg ccggatgagt gcattccgtt tgaccatctt ctgcacgcca
     1681 atcgccagcg aaatagtcac caccgcgggc agagcttcgg gcaccaccgc caccgccagc
     1741 gcaatgccga agatcagcat ttccaccagc ggctgaccgc gcaccagccc caccgccaca
     1801 atcagcgcca ccaccacaaa agcagcccgt gccagaaccg cccccaactt gtccaggttg
     1861 tgctggagcg gggtcttgcc ggtttcaacc gtttgcagca tttgcgcaat tttaccgaac
     1921 tcggtctgca tacccgtcgc agctaccagg gcccgcccgc gtccataggt cactgccgtc
     1981 ccggcataga ccatattctt gcggtcaccc accgacagct cagagttatc cagcggctgc
     2041 gtgtgcttct caatcggcat tgattcaccc gtcagcgctg cttcttccac ctgcaggttc
     2101 accgcctcca gcagccgtgc atcggctggc acgcggtcgc ccgtgttgag tagaatcaca
     2161 tcgccaggaa ccagttcgcg cgccgggatt tccgtgtcgt cgccatcgcg cagcactgtg
     2221 gcagtgggcg ccgccatctc ccgcagcgct tcaatcgccc gttccgcgcg atattcctgc
     2281 acgaagccca gcgccacagc aaacagaacg atcaccgcaa tcacaactga ctcaatcccg
     2341 tgccccagga acagcgaaat aatcgttgct cccagcagaa tcagaatcag gatattcttg
     2401 aactgcgcca gcagaatttc aaaggccgaa atccgcttgg ccgcctgaat ctcattgggg
     2461 ccaatttctg ccaggcgttt ggctgcctcg gcgctggtca gcccctgcgg actcgtctcc
     2521 agtgcggcgt taatctcgtc ttgtttcagg gtatgccaga ttttagcttc cataaatatc
     2581 gtctttctcc ttgttgttta gtttttgtaa gataacaggt ggaggccttg cccaaaaaca
     2641 ccggccttac tattcatcct caccgggcgc cagcaccaga gccggggccg cccgtctccg
     2701 tgtccaccag aagtagattc ccaatgcaac ggcccctccc accagcaggt agggcagggc
     2761 ctgcttcaaa tacaagctga ttaactccca ctcatgcccc agcaggtacc cggcgccaat
     2821 caacagggta caccaggcat acgcgccgat gaatgtggtg gtcacaaaag tcccaaaggg
     2881 catgcgcgcc agccctgccg gaatgctgat cagcgtccga atggcgggga tcattcgccc
     2941 aatcagtacc aatcccgtcc cccagcgctg aaagagctgc tctgcccggg caatatgctc
     3001 cggcgcaatc cgcacccagc gcgcaaaccg gtcaactacc ggacgcccac ccagccgcgc
     3061 cacccagtag gtgatggagg ctccaatcac gctgccgata gctgaataca gtccacccag
     3121 aaaaatcatg ctgaccggca gctcgtgttc ggcaatcagc atccaaccgg caaagcccag
     3181 gatcacctcg ctgggtgtga tgccggtggc attttcaaaa accagcaacg ctgccacccc
     3241 cagccagccc caggcattat acagcgtttg cagcaatcct aaaatccaga tttcaatttg
     3301 atcaatcatt caaccgatcc ttgttggggc ggggtggtgg ttaacgccgc gggttctggc
     3361 agacggtttt taacccggct gagatgggtg gtgacgccaa cggcgataac gaacagcagg
     3421 gctttgagcc actcgctatc cacgataaac aggatggcag tgccaatcgt caaccacagc
     3481 agcgtcaggg tgattagttt ttgccgcagt ggaatgccct tgccgtctcg atagtcgcgg
     3541 atatagctgc caaaaggcga gcggtatacc agccactggt aaaacccttc agatccgcgg
     3601 ccgtaacaaa aggccgccag cagtaaaaat ggcgtggttg gcagaaccgg gaggatgatt
     3661 ccaataatcc ccagccccag acagattgtc ccaccgatga tccacagttt tttacccatc
     3721 acaggcgcca tcctcgtctc aaaataaaaa accatcgtca aaaaaacgat ggtcttgcgc
     3781 ttgagttcag acagccggga aataaattcc gtcttgacga ccgtctatcc cgaaaaatcg
     3841 ggaggctact ccccaccgga gttgagatag tttacaggat ttatatacaa ttgtcaatcc
     3901 ttttaaggtc gccgccatga ccagttattg gataaaaatc cacccggatc agaaaggccg
     3961 attattcctg aaccgggtgg tttgatgacc tgggaggttt gcccgttacg gttttatttc
     4021 aacccgcact acccgcacac tcagcccgcc ttcggctggt gttgccgtga taaccagcat
     4081 ggtctgcacg ccctcaccct gctggatata agcccacagt gggccattgg ctgtttcaag
     4141 gccctcgccc tgcggcaggt aacccagcgc acccaactga tcggtataga aggctgccag
     4201 cgtaccggca tcaagcgccg tctggaagcg aacctcagcg ggcagctcta ccagcccggt
     4261 agccccatct ggaactggga tccccctcag cggcaggggg cagctttctg gaagctgcat
     4321 ggcaccctca ccggggtccc gcagggtata ttcccacgac tgctgtcccg tgattccggc
     4381 gccaaagacc gtatcagact ctaacgtcag cgtatatttg atcacctgtc cgcccggctg
     4441 ggcaacccag accttgccgc tggcggtggc ggcctctccg gctccga
//
LOCUS       DAOPVS010000157         4451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_19690_length_4451_cov_43.991811, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000157 DAOPVS010000000
VERSION     DAOPVS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19690_length_4451_cov_43.991811"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /gene="ugpC"
                     /locus_tag="PKL11_09680"
     CDS             complement(<1..967)
                     /gene="ugpC"
                     /locus_tag="PKL11_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNZ13914.1"
                     /translation="MASVTYDHVWKKFGDFTALNDLNIEIKDKEFLVLVGPSGCGKTT
                     ALRCLAGLEEVSSGKILIGDRVVNDVPPKDRDIAMVFQSYALYPHMTVYDNMAFGLKL
                     RKMSKEDINRRVLEAAGVLGIEHLLKRRPRELSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQTRAEISKLHLRLQTTFIYVTHDQTEAMTMASRIAVMNKGVLQQLDTP
                     QTLYDFPANQFVAGFIGSPSMNFFSAKLRKDGEKLFVDGDGFAVEIPAPRVAPYMSYV
                     GKDVIFGIRPEDIFNPQFLPPGIHTAPVEAKVDVTELMGNEIFLYL"
     gene            1139..1810
                     /locus_tag="PKL11_09685"
     CDS             1139..1810
                     /locus_tag="PKL11_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13915.1"
                     /translation="MMDKNMILALIPGYNEAQRIAAVVNTAGTHLPVLVVDDGSSDNT
                     RGVAEAAGAQVIRQEPNQGKGAALRAGFRWALANGYEAVITLDADGQHDPAEIPGFLD
                     AYAQDKSDLIIGARDFSQMPAVRRWANTTGQKLFSWALGQPVQDNQSGYRLISRRLME
                     AVLESAESGFEFEVEMIVVCVRGGMKLDWVPIRTIYGDQGSHIKPLHHAAHFMRIIWN
                     TWRRR"
     gene            complement(1807..2265)
                     /locus_tag="PKL11_09690"
     CDS             complement(1807..2265)
                     /locus_tag="PKL11_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13916.1"
                     /translation="MENQHTHALAVYQACEDFLALGPEPHASAAMVAADLQYSREHGG
                     IYSGIWHLETLVGVLDYIPSHYEGQPHCAFINLLMIIPSSRGSGLGHSVIAWLEQQLS
                     AQGITQVWTAVQVNNPLALSFWQHCGYAIKTPPVRQSDGTTTQLLAKSLA"
     gene            2353..2868
                     /locus_tag="PKL11_09695"
     CDS             2353..2868
                     /locus_tag="PKL11_09695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ13917.1"
                     /translation="MPTNLSWPKPPELEIDLKKSYTATFHTDKGDIVIKLHADRVPRT
                     VNNFVFLARQGFYNDTIFHRVINDFMAQGGDPTGTGMGGPGYKFSDEFDPKLRHDKPG
                     VLSMANAGPNTNGSQFFITHVPTPWLDGKHSVFGQVIQGMDVLMSIPARDPQRVGAPA
                     VKLISVTITEE"
     gene            complement(2948..>4451)
                     /locus_tag="PKL11_09700"
     CDS             complement(2948..>4451)
                     /locus_tag="PKL11_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ13918.1"
                     /translation="MDMGAPIITLGNFLVALGLGLGITVLAGLIPALSASKIPPIEAL
                     RLESVGQVARKVRTRAIIGFVMIALAVIALITGSFNLAALGAVLFLVGVILSAPVLVR
                     PLAETFGRLLNIFFAREGHLAQRNLARQPERAAVTALAMLIGLSIVVALAGVLSSTFG
                     GFLSYLDRSLGADYLVMPTSLMLGGGNMGAAPGLAAELSQVEGVAGVTTLRLAYGKVD
                     GTDLQVIGIDPQTYPVIAGLEFSRGEPAEAYAALDQERAIIVNGIFAATTGVQIDDLV
                     TIMTPSGEQQYRIVGVGLDYLNAKLTTGYISQANLERDFLTTTDVLLMANAVDGADKS
                     TVGARLTNLISAYPAFTLLESDTFYQAQVNIFKAALAMMYVMMAAMALPALIAMMNTL
                     AINVIERTREIGVLRAVGSTRKQIRRMILAESLLLALFGTGLGILAGVWLSYVLVGAM
                     NFSGFVMQFNFPWGGVLAGLAVGLIFGVLAATLPARQAARLDIIHALRFE"
BASE COUNT         1075 a         1146 c         1355 g          875 t
ORIGIN      
        1 ccaggtagag gaagatttcg ttacccatca gttcggtgac atccactttg gcttcgaccg
       61 gggcggtatg gatgcccggg ggtaggaact ggggattgaa aatatcctcg ggacggatgc
      121 cgaagatgac atctttgccg acataggaca tatagggagc cacgcgaggg gcggggattt
      181 caacggcaaa gccatcacca tccacaaaga gcttttcgcc atctttgcgc agcttggcag
      241 agaagaaatt catcgagggg gagccgatga agccggcgac aaactggttg gcggggaaat
      301 catacagggt ctggggagta tccaactgct gcaggacgcc tttgttcatg acggcgatgc
      361 gggaagccat ggtcatggct tcggtctgat cgtgggtgac gtagatgaaa gtggtttgca
      421 ggcggaggtg cagcttgctg atttcagcgc gggtctgcac gcggagcttg gcatccaggt
      481 tcgagagcgg ttcatcgaag aggaagacct ttggctcgcg gacaatggcg cggccaacag
      541 ccacgcgctg gcgctgacca ccagagagct cgcggggtct gcgcttgagc aaatgttcga
      601 tgcccagcac accggcagcc tcgagtacgc ggcggttgat gtcttctttg gacatcttgc
      661 ggagcttgag gccaaaagcc atattgtcat acacggtcat gtgcgggtaa agcgcgtacg
      721 actggaacac catggcaatg tcgcgatctt tgggcggcac gtcattgacc acgcggtcgc
      781 caatcaggat tttgcccgag gaaacttcct ccaagccagc cagacagcgc agcgcagtgg
      841 ttttaccgca gcccgaaggg ccgaccaaaa ccaggaattc tttgtcctta atttcaatat
      901 taaggtcgtt cagggcggtg aagtccccaa actttttcca gacatgatca taagtaacgc
      961 tagccataca gtcctccttc cagagcgaat atagtgattt gattatatgc cccaataaaa
     1021 gaaaatgcaa cagtatttgt caagggattt aaaaattaga ttaaagaaaa acaaggaatc
     1081 gctgaacgtg tatcaattgc gggattgggt ctgaggataa gcaagtataa taacgaacat
     1141 gatggacaaa aacatgattc tggcattgat tccgggatat aacgaggcac agcgcatcgc
     1201 ggcagtggtg aacactgccg gcactcacct gccggtgctg gtggtagatg atggatcgag
     1261 tgataacacc cggggggttg ctgaagccgc cggggcacag gtgattcgac aagagcccaa
     1321 ccagggtaag ggggctgcac tgcgggcggg cttcagatgg gcgctggcga atgggtatga
     1381 ggcagttatt accctggacg ccgatgggca gcatgatccg gcggagattc caggtttttt
     1441 ggatgcctat gcgcaagata agtccgattt aattatcggg gcgcgggatt tttcgcaaat
     1501 gccagccgtg cggcggtggg ccaacaccac cgggcagaag ctgttttcgt gggcgctagg
     1561 acagcctgtg caggataatc aatcgggata ccggttgatc agccgcagat tgatggaagc
     1621 ggtgctggag agcgctgaaa gcggttttga atttgaagtg gaaatgattg tggtttgtgt
     1681 gcggggcggg atgaagctgg actgggtgcc catccgcact atttacgggg accagggcag
     1741 ccacattaaa ccgctgcatc atgcggcgca cttcatgcgc atcatctgga atacctggcg
     1801 gagacgctag gcgagagatt ttgccagaag ctgtgtagtg gtgccgtcag actggcgtac
     1861 aggcggggtc ttgattgcat aaccacaatg ctgccaaaaa gacagcgcta aagggttgtt
     1921 cacttggacg gcggtccaga cctgggtgat tccctgtgca gataattgct gctccagcca
     1981 agcgatcacg gaatgcccaa gcccactccc ccgcgaggaa gggatgatca tcaaaaggtt
     2041 gataaaggcg cagtgcggct ggccttcata atgcgatggg atgtaatcca gcacaccaac
     2101 cagggtttct aaatgccaga tgccggaata gatgccgcca tgctcgcgag aatactgcag
     2161 atctgcggct accatggcgg ccgaggcgtg cggctcgggg cccagggcaa gaaaatcctc
     2221 acaggcctgg tagactgcca gggcgtgggt gtgttgattt tccaaaagtg gtgatagaat
     2281 ccattgcatc aactggatta aaccacattt tgatccaggt caccttccaa aattaactcg
     2341 aaaggatctg caatgcctac gaatctatcc tggcccaagc cacccgaact ggaaattgac
     2401 ctcaaaaaat cttataccgc cacattccat acagacaagg gcgatattgt catcaagctg
     2461 catgccgacc gcgtgccccg gacagtgaat aattttgtgt tcctggcccg gcagggtttt
     2521 tacaatgaca cgatcttcca ccgcgtgatt aacgatttta tggcccaggg cggcgacccc
     2581 accggcacgg gtatgggcgg ccctggctac aagttcagtg atgagtttga ccccaagctg
     2641 cggcacgata agccgggtgt gctttcgatg gccaatgccg gaccgaacac caatggttcg
     2701 cagttcttca tcacgcatgt gcctaccccc tggctggatg ggaaacacag cgtgtttggg
     2761 caggtcattc agggcatgga tgtgctgatg tcgattccag cacgcgatcc gcaacgggtt
     2821 ggggcgccag ccgtgaagct gatttcggtg acgatcaccg aagaataatt gcttctgaca
     2881 agtaaaatga acgaggcgga gcacaatgct ccgcctcgtt ttttcaccag aatggacagg
     2941 ggcaatatca ctcaaagcgc agggcgtgga tgatatccag gcgggcggcc tgacgggcag
     3001 gcagcgtggc agccagcacg ccaaaaatca aaccaacggc aaggccggcc agcacgcctc
     3061 cccaggggaa gttgaactgc atgacaaagc cgctgaagtt catggcgccg accaacacat
     3121 agctgagcca aacgccggcc aggatgccaa ggcctgtgcc gaacaaagcc agcaggaggc
     3181 tctcagccaa aatcatccgg cggatttgtt tacgggtact gcccacggca cgcagcacac
     3241 caatctcgcg ggtgcgctca atcacgttga tcgccagggt attcatcatg gcaatcaggg
     3301 cgggcagcgc catggcagcc atcattacat acatcatcgc cagggcggcc ttgaagatat
     3361 tcacctgagc ctgatagaag gtatcggatt caaggagtgt aaaagcaggg taggcagaga
     3421 tcaggttggt gaggcgggcg ccaacggttg atttatcggc cccgtcaacg gcattggcca
     3481 tcaggaggac atcggtggtg gtgaggaaat cacgctccag gttcgcctgg gagatgtagc
     3541 cggtggtcag cttggcgttg aggtagtcga ggccaactcc gacaatgcgg tattgctgct
     3601 cgcctgatgg cgtcataatt gtcaccagat cgtcaatctg gacgccggtt gtggccgcaa
     3661 agatgccatt gacgatgatg gcgcgttcct ggtcgagggc agcgtaggcc tccgccggtt
     3721 caccgcggct gaattccaac ccggcaatga ccgggtaggt ttgcgggtca atgccaatca
     3781 cctgcaggtc agtgccatcc accttgccat aagcaaggcg gagcgtggtg accccggcga
     3841 caccttcgac ctgactcagt tcagccgcca ggcctggggc tgcgcccatg ttccctccgc
     3901 ccagcatgag cgaggtgggc atgaccaggt aatcagcccc gaggctgcga tccagatagc
     3961 tcaaaaagcc gccaaaggta ctggagagga ccccggcgag ggcaaccacg attgaaagac
     4021 cgatgagcat ggccagggcc gtcactgcgg cacgttctgg ctggcgggca aggttgcgct
     4081 gggcaaggtg cccttcgcga gcaaagaaga tgttcagcag gcggccaaag gtttcagcga
     4141 gcgggcgcac caggaccggc gccgacaaaa tgacgcccac cagaaagaga acagcaccca
     4201 acgccgcaag gttaaagctg ccggtaatca gggcaatcac ggcgagcgcg atcatcacaa
     4261 agccgatgat ggcgcgagtg cggactttcc gggcgacctg tccgactgat tccagacgca
     4321 gggcttcaat gggtgggatt ttgctggctg aaagcgccgg gatcaggcca gccagaacgg
     4381 taatgcccaa tcccaagccc agtgcaacca ggaagtttcc cagggtgatg atgggagcgc
     4441 ccatatccat a
//
LOCUS       DAOPVS010000158         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_20028_length_4398_cov_48.852867, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000158 DAOPVS010000000
VERSION     DAOPVS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20028_length_4398_cov_48.852867"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..793)
                     /locus_tag="PKL11_09705"
     CDS             complement(77..793)
                     /locus_tag="PKL11_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13919.1"
                     /translation="MMEIAAAVDLFWRCCTRQYRAHWGTKNYLINDLRVVEYAQLLYG
                     APYEINVTDEPADQVMAVVHQLPERCWILVFTPDMAASQMAYAAYNLAVDDEIERMMW
                     RSLEPLPLKPSILVQQVTTPAQLESLCLAREVRMFDWADFASGDVIMCYASVDDQPAA
                     WGAAIFLNPGVAYIAWMFTCPEYRQRGLAAAVLTQLMHLSAERGAVESLLVASPMGLN
                     VYEHLGYSRLCDLMVLGESA"
     gene            complement(798..2477)
                     /locus_tag="PKL11_09710"
     CDS             complement(798..2477)
                     /locus_tag="PKL11_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HNZ13920.1"
                     /translation="MSTFSHLLHQQYLHTPDRNALTLLRAGQPDLPLTVRELLQGSAG
                     VAQTLAQHGIQPGEVVILILQHSLEHAFAYFGTILHGAIPSIMPFLTEKLLPERYRAD
                     LAALVGVTQPSAIITYPEFEGEVRAALGESSSVRVVLLSTEMDPPREPDFASLPGLQR
                     SPEDIVLLQHSSGTTGLQKGVALSHRAVLNQLESYSRRLQLNEADVLVSWLPLYHDMG
                     LIAGFLMPLLLGIPLVLMSPFDWVRNPARLMQAVSKYRGTLSWLPNFAYNFCAQKTRD
                     RDLEGVDLSSWRAISNCSEPMRYESHQAFLARFAPYGLRPEALCTCYAMAENVFAVTQ
                     GGITEPVTVDVIDREAMQVERVARPTLTDEAVIHMLSAGRPIESVEVRVVDEHGHDVP
                     ERKIGEVCLRSNSMLTEYYHRPDATAKAFRSGWYLTGDYGYLAAGELYVTGRKKDLII
                     VGGKNIHPQDLEELAMTVPGVHAGRVVVFGIFNDEQGTEEVYLVAEVDPEDPAAREQI
                     ADQIRQTITRGSAVALRHVHLVNKHWLVKTSSGKTARGANREKYIKEVLGQ"
     gene            complement(2474..2719)
                     /locus_tag="PKL11_09715"
     CDS             complement(2474..2719)
                     /locus_tag="PKL11_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ13921.1"
                     /translation="MSAEMITSMAEYLAKNILKQPQRVIRPDEPLISSGLIDSFSLVD
                     LGLYVEDTFGVRLEDTELNAETFDTLTQLVELVKGRM"
     gene            complement(2882..>4398)
                     /locus_tag="PKL11_09720"
     CDS             complement(2882..>4398)
                     /locus_tag="PKL11_09720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ13922.1"
                     /translation="LIIWSVTLQAFPKDIQLASPLVQNNPYRVRPLKEQYDLDLPVET
                     LSEPNFWQRTLIGQRRSLADWLRLQLYAPMWSATGIDQLYPADYEPAQRDLDPDETFN
                     NWSGPDLGFEQLSFDVLSAGFEIAGETPVLLVNEPILISAGQNSDLRYNFYYPRWAYD
                     SYRAMLGETAGLNAWSYLDLWNLVPESQFTNSAIHLTPQGVSMMAEAVGDALNRMSCG
                     QSAQPPAPTPTPTATPLPATATPVAVLSPTPTPTPTRPVYTDWRDYPVLPTVSDTARE
                     IYQRGLALGNNPAAFSKVGDCETGSDWFLRTFDVQPPLYRLGEYEYLQEMILYFDGSF
                     ARRSIVAKNGFNAATALSPIWADPEVCLSGETPLACEYRIHKPSIALIAFGTNDTKNP
                     ARFEGDLRKIIEITIEAGIVPVLSTKADNLEGDNSINETIVKLAVEYDIPLWNFWAAI
                     QDLPDAGLRADKMHLTWAQNYFDDPETMEKAWPWRNLTALQTLDIVWRGLQETP"
BASE COUNT         1006 a         1131 c         1388 g          873 t
ORIGIN      
        1 ccggctttca gtaacgcgtg ctagtaaaag tatacaggca agccctattt taatcaaatt
       61 taattcagga tgaaaatcag gccgattctc ccagaaccat caggtcgcag aggcggctgt
      121 agcccagatg ttcatagacg ttcaggccca ttggcgaggc aacaagcagc gattcaaccg
      181 caccgcgttc ggcggagaga tgcatgagct gcgtgaggac agccgcagcc aggccgcgct
      241 ggcggtattc cgggcaggtg aacatccagg cgatgtaggc aaccccggga ttcagaaata
      301 tggcagcacc ccaggcggct ggctggtcat ccaccgaggc atagcacatg atgacatcac
      361 cactggcaaa atctgcccag tcaaacatgc gaacttctcg ggcgagacac agcgattcca
      421 actgggcggg tgtggtaacc tgctgaacga ggatggacgg tttgagcggg aggggctcca
      481 gggagcgcca catcatgcgc tcaatctcat catcgacggc gagattatag gcggcataag
      541 ccatctggct ggcagccata tcgggggtga ataccagaat ccagcaccgc tcaggtaact
      601 gatgcacaac tgccataacc tgatcggcag gctcgtcggt gacgttaatt tcatagggcg
      661 cgccatagag caactgggcg tattcaacta cccgaaggtc attgatgagg taatttttcg
      721 tgccccagtg ggcccgatac tggcgggtgc agcaccgcca gaaaagatca acagcggcgg
      781 caatttccat catcgggtta ctgacctaaa acttccttga tatatttctc gcgattggca
      841 ccgcgagcgg ttttgccgct actggtcttg accagccagt gtttgttgac caggtggacg
      901 tggcgcaggg ctaccgccga gccgcgggta attgtctgac ggatctgatc ggcaatctgt
      961 tcacgggcag cggggtcttc tggatccacc tcggcgacca gatagacttc ctcggtacct
     1021 tgctcatcgt taaaaatgcc aaagaccacc acacggcctg cgtgaacgcc ggggaccgtc
     1081 attgccagtt cttcaagatc ctggggatga atatttttac cgccaacaat aatcaaatct
     1141 ttcttgcgac cggtgacgta tagttcacca gcggccagat agccgtaatc acccgtgaga
     1201 taccagccag agcggaaggc tttggcggta gcatcgggtc ggtgatagta ttcggtcaac
     1261 atgctgttgc tgcgcagaca aacttcacca atcttgcgct cggggacatc atggccgtgt
     1321 tcgtccacca cgcgcacctc aacgctttcg atggggcggc ccgccgaaag catatgaatg
     1381 acggcctcgt cagtgagggt gggacgagcc acacgctcga cctgcatggc ctcgcggtca
     1441 atcacatcaa ccgttacggg ttcggtgata ccgccctggg tgacggcaaa gacattctcg
     1501 gccatagcgt agcaggtgca aagcgcctcg ggacgcaggc cgtagggggc aaaacgcgcc
     1561 agaaaggcct gatggctctc gtagcgcatc ggctcggagc agttgctgat ggcccgccaa
     1621 ctggacaagt ccacgccttc caggtcacga tcacgggttt tctgggcgca gaaattatag
     1681 gcaaagttgg gcagccaact gagtgtgccg cggtatttgc tgactgcctg catcaggcgg
     1741 gcgggattgc gcacccagtc aaagggggac atgagaacca gcgggatccc cagcaggagg
     1801 ggcatgagga agccggcaat caggcccatg tcgtggtaca gcggcagcca gctcaccagc
     1861 acatcagcct cgtttagctg caaacggcgg gaatagcttt cgagttgatt gaggacagcg
     1921 cgatgcgaga gcgccacgcc cttctgcaga ccagtggtgc cggaggaatg ctgcagcagg
     1981 acaatatctt cagggctgcg ctgcagaccg ggcagcgagg caaaatccgg ctcacggggg
     2041 ggatccattt cagtagagag cagtaccacg cgcaccgatg agctttcacc gagggcagcg
     2101 cggacttcgc cctcaaattc gggataggtg atgatggcag aaggctgggt gacgcccacc
     2161 agagccgcca gatcggcgcg gtaacgctcg ggcaggagct tttccgtcag aaaaggcatg
     2221 atggaaggaa tggcgccgtg cagaatcgtt ccaaaatagg cgaaggcatg ctccaggctg
     2281 tgctggagga taaggatgac cacctcgccg ggctggatgc catgctgcgc cagggtttgc
     2341 gccacgcccg cactcccctg gagcaactcc cgcacggtca acggcagatc aggttgaccg
     2401 gcgcgcagca gagtgagggc attgcgatcc ggggtgtgca aatactgctg gtgcagtaaa
     2461 tgtgagaagg tactcacatg cgccccttga ccagttcgac aagctgggtc agggtatcaa
     2521 aggtttcagc attcagttcg gtgtcttcaa ggcgaacccc aaaggtatcc tcgacataca
     2581 ggcccaggtc caccaggctg aaggaatcaa tcaggccgct ggagatgagc ggttcatccg
     2641 ggcgaatgac gcgctggggc tgcttgagga tatttttagc cagatattcg gccatgctgg
     2701 taatcatttc ggcactcatg gggtgtcctc cgtaaattca aatttgaact gggctttagt
     2761 gtaccgaaga ttgaaaatcc ggtcaagtaa agcactggtt atgcagtcaa aacaggtttt
     2821 tgccagtgca aagcaccagc aaaaacctgg aagacgggca agcgtgaagg aagatggcag
     2881 gtcagggagt ttcctgcaga ccgcgccaga cgatgtcgag ggtttggagg gcagtcaaat
     2941 tgcgccaggg ccaggctttt tccattgttt cagggtcgtc aaagtagttc tgcgcccagg
     3001 taaggtgcat tttgtcggcc cggagacccg catcggggag atcttgaatg gcggcccaga
     3061 aattccagag ggggatatcg tactccaccg ccagcttgac gatggtttcg ttgatactgt
     3121 tatcgccttc gagattatcg gctttggtgg agagaaccgg tacaatgcca gcttcaatgg
     3181 ttatttcgat gatcttgcgc aagtcgcctt caaaccgggc agggtttttg gtgtcgttgg
     3241 tgccaaaggc gatcagggca atggatggtt tgtgtatgcg gtattcgcag gccaacgggg
     3301 tttcgcctga aagacagacc tcggggtcag cccagatggg gctgagggca gtggcggcat
     3361 tgaagccgtt tttggcaacg atgctgcggc gagcaaatga gccgtcaaag tagaggatca
     3421 tctcctggag atattcatac tctcccaggc ggtaaagcgg cggctggaca tcaaaggtac
     3481 gcaggaacca gtcactgccg gtttcgcagt cgccaacctt tgagaaagca gcaggattgt
     3541 tgcccagggc aaggccgcgc tggtagatct cgcgggcggt atcgctgacg gtgggcaaaa
     3601 cggggtaatc gcgccagtcg gtatagactg gccgggtagg tgtgggggtt ggggtgggtg
     3661 aaagcaccgc cacaggtgtg gctgtggccg gtaacggggt ggcggttggt gtgggggttg
     3721 gcgccggagg ctgagccgat tggccacagc tcatccggtt gagggcgtcg ccgacggcct
     3781 cggccatcat ggaaacgccc tgtggggtga gatgaatggc gctgttggtg aactgagatt
     3841 caggaaccag gttccagagg tccagataag accaggcatt caaaccggcg gtttcaccca
     3901 gcatggcgcg gtaggaatcg taggcccagc gcgggtagta gaagttgtag cgaagatcac
     3961 tgttttggcc ggcgctgatg aggatgggct cattgaccag caagaccggg gtttcaccgg
     4021 caatctcaaa gccggctgaa agtacatcaa aagaaagctg ctcaaagccc agatcagggc
     4081 cagaccaatt gttaaaggtt tcatcagggt caaggtcgcg ctgggcgggt tcatagtcgg
     4141 cggggtagag ctggtcgatg ccggtggcgc tccacatggg ggcataaagc tgcaggcgca
     4201 gccaatcggc cagtgagcgg cgctgaccga tcagcgtgcg ctgccagaag ttgggctcgg
     4261 atagggtttc aaccggcagg tccaggtcat actgttcttt caacgggcgg acacggtagg
     4321 gattgttctg caccagcggc gaggccaact gaatatcttt gggaaaagcc tgcaaggtca
     4381 ccgaccagat gatcagat
//
LOCUS       DAOPVS010000159         4395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_20047_length_4395_cov_61.838018, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000159 DAOPVS010000000
VERSION     DAOPVS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20047_length_4395_cov_61.838018"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKL11_09725"
     CDS             complement(<1..446)
                     /locus_tag="PKL11_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13923.1"
                     /translation="MLAGRTKERATYWRFLLLLMLLAAALVLGLILVDAELRLSGLSG
                     LLEVPLHSPFQADYSADPRGQPIAAMNDSLLRDLFEPGFLPTLTAFVPTITPAGPLAA
                     TPTPAAPIFTPLPTSTAAPGMPSSTPLPTATLTATPLPTWTATLPG"
     gene            complement(505..2700)
                     /locus_tag="PKL11_09730"
     CDS             complement(505..2700)
                     /locus_tag="PKL11_09730"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNZ13924.1"
                     /translation="MPLRVLIIQSEPKDAQELTRFFKERGDEVWGAWNLGQADGLLDQ
                     IRPGLIVLDIHLPDSEWTNFLKRATQRTGSQVIVTSRHPDMTREMMAQSAGVSAFLRA
                     PFKPEWIERALGRMGQRTVPRTKPILPKVRTPVRLKITVPYLLLSLLLALGVAFIVSQ
                     VVVDSAEERYLNQLIETSRQSADWMVSEEDRLLITLRLVANSSGVAEAIQAGSSETLR
                     AQVLPLAANAREESIEILDLSGTSLLSIRQTPGEAAGVYSFTRGDTTFQTADYVLAVL
                     QGQVDNRGDKFAGLTIGPWGRYFYVSGPVFNPTGQMVGVVLVGRSLDTLAREMRTDTL
                     GEITFYGIDGQPLASTLYHGADSFPLSTEKVSLVLAAQDVASQLRDLTVSSIDYAELL
                     GPWEVRDGRDLGVLGVTQPKAFLIQTSRITRWEIFVFVVAAVMLVVGVGFYLASIITR
                     PLQRLMKASNQVAEGNLEVKVDVRGDDEVAVLAHSFNTMIAGLQEGSIYRDLLGRTVS
                     PEVREQLRQTFSSGNLRLEGQEAVSSVLMTDIRAFTTLSEQADPSKVFTWLNEYFGKL
                     VPIVVNHGGVVNKFDGDAMLAFFGILPRITNPRKSAMAACETAVEMMRAIDELNALRA
                     QRREPVMVTGIGITTGVVIAGGLGSSDRLHYTIIGDTVNTASRLQGLTRELCSESAII
                     VGQTTLDALGEQQNLFLFEALGEHNVRGKAEILQVHRLHVPPRPTDRRL"
     gene            complement(2730..3584)
                     /locus_tag="PKL11_09735"
                     /pseudo
     CDS             complement(2730..3584)
                     /locus_tag="PKL11_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(4011..4322)
                     /locus_tag="PKL11_09740"
                     /pseudo
     CDS             complement(4011..4322)
                     /locus_tag="PKL11_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
BASE COUNT          837 a         1298 c         1442 g          818 t
ORIGIN      
        1 tggcccggca aagtggccgt ccaggttggc agcggggtgg cggtcagcgt ggcggtgggc
       61 aggggggttg agctgggcat gccaggggcg gcggtgctgg tgggcagcgg cgtgaagatc
      121 ggcgcggccg gggtgggggt ggctgccagc ggccccgccg gggtgatggt aggcacaaaa
      181 gcggtcagcg tgggtaaaaa cccgggctca aacaggtcgc gcagcagcga gtcgttcatc
      241 gcggcaatcg gctgaccgcg cgggtcggcg ctgtaatcgg cctggaaggg ggaatgcagg
      301 ggcacttcca gcaggccgct cagcccgctc aggcgcagct cggcatccac caggatcaag
      361 ccaagcacca gggcggctgc cagcagcatc agcagcagca aaaaccgcca gtaagttgcc
      421 cgttctttgg ttcgaccagc cagcacaacg acctccgcgc ctcaagtata gcggatggaa
      481 agacagaacg aaatggccgg gagattacaa tctcctatca gttgggcgcg gcggcacatg
      541 caggcggtga acctgcaaaa tctcggcttt gccgcgcacg ttatgctcgc ccagggcctc
      601 aaacaggaag aggttttgct gctcgcccag ggcatccagg gtggtctgac cgacgataat
      661 ggcgctttct gaacacaact cgcgcgtcag gccctgcagg cgcgaggccg tgttcaccgt
      721 atcgccaatg atggtgtaat gcagccgatc gctgctgccc agaccgcccg caatcaccac
      781 gccggtggtg atgccgatgc cggtgaccat cacgggctcg cggcgttgcg cgcgcagcgc
      841 gttcagttcg tcaatggcgc gcatcatctc cacggcggtt tcgcaggcgg ccatggcgct
      901 cttgcgcggg ttggtgatgc gcggcagaat gccgaaaaat gccagcatgg cgtcgccgtc
      961 aaatttgttc accacgccgc cgtggttgac cacaatgggc accagtttgc caaaatattc
     1021 gttcagccag gtgaacacct tggaagggtc ggcctgttca gacagggtgg tgaaggcgcg
     1081 gatgtcggtc atcagcacgc tggagacggc ctcctgacct tccaggcgca ggttgcccga
     1141 gctgaaggtc tggcggagct gctcacgcac ctctggcgag acggtgcgcc ccagcagatc
     1201 gcggtagatg gagccttcct gtaacccggc aatcattgta ttaaatgaat gtgccagcac
     1261 cgccacctcg tcatcgccgc gcacgtccac cttcacttcc aggttgccct cggccacctg
     1321 gttagaggcc ttcatcaggc gctgcagcgg gcgggtgatg atgcttgcca ggtaaaagcc
     1381 cacccccacc accagcatca ccgcagccac cacaaagacg aaaatttccc agcgggtaat
     1441 ccggctggtt tgaatcagaa aagcctttgg ctgcgtcacg cccagcacgc ccaggtcgcg
     1501 tccatcgcgc acctcccagg ggcccagcag ttcggcatag tcaatgctgg atacggtcaa
     1561 atcgcgcagt tggctggcaa catcttgagc cgccagcacc aggctgactt tttcggttga
     1621 gagggggaag ctgtcggcgc catggtagag ggtggaagcc agcggctgac catcaatacc
     1681 ataaaaagtg atctctccca gggtatcggt gcgcatctcg cgcgccagcg tatccagcga
     1741 gcggcccacc agcaccacgc ccaccatctg ccctgtgggg ttgaataccg gcccgctgac
     1801 gtaaaaatag cgcccccagg ggccaatcgt cagaccggca aatttatcgc cccggttgtc
     1861 cacctggccc tgcagcaccg ccagcacata atccgccgtc tgaaaggtgg tatcgccgcg
     1921 ggtgaaggaa tagaccccgg ccgcttcgcc gggcgtctgg cggatggaca gcaggctggt
     1981 gccgctgagg tccaaaattt caatcgattc ttcgcgggcg ttggccgcca gcggcagcac
     2041 ctgggcgcgc agcgtttcag agctgcccgc ctggatggcc tcggccacgc cgctgctgtt
     2101 cgccaccagg cgcagggtga tcagcaggcg gtcttcttcg ctgaccatcc aatctgccga
     2161 ctggcgggag gtctcgatca actggttgag gtagcgctct tcggctgaat ccaccaccac
     2221 ctggctgacg atgaacgcca cgcccagcgc cagcagcagc gaaagcagca gatagggcac
     2281 ggtgatcttc aagcgcacgg gggtgcgcac cttgggcaaa atcggtttgg tgcgcggcac
     2341 cgtgcgctgc cccatccgcc ccagggcgcg ctcaatccat tcgggcttaa acggcgcccg
     2401 caaaaaggcc gaaacccccg ccgactgcgc catcatctca cgggtcatgt cggggtggcg
     2461 gctggtgacg atcacctgcg agccggtgcg ctgggtggcg cgcttgagga agttggtcca
     2521 ctcgctgtca ggcaggtgga tatccagcac aattaatcca ggtcgaatct ggtcgagcag
     2581 gccatcagcc tggcccaggt tccaggcccc ccacacctcg tccccgcgct ctttgaagaa
     2641 gcgggttagt tcctgggcgt cctttggctc cgattggatg atcaatactc gcagcggcat
     2701 tggacacctt ggggttgtaa agaacgatgc taggataaat attcgcgcag gtgacggctg
     2761 cgggtaggat gccgcagttt gcgcagcgct ttggcctcaa tctgacggat tcgttcgcgg
     2821 gtgacgttaa agtagcggct gacctcttcg agggtatggt ctttgccatc aatcaaaccg
     2881 aagcgcagct ccagcacctg gcgctcgcgt tcagacagcg cccccagggc attttgcacc
     2941 tggtcgcgca gcatttcgcg ggcggccgcg tccatcggct ccagcgcgtc gtcgtcttcg
     3001 atgaagtcgc caagctggct gctttcttca tcaccgacgg gccgctccag cgagacgggc
     3061 tcttcggcag actgtaaaat gcgctgaact ttggccgtgg cggttaccca acgatgttgc
     3121 aagtcttgtt ccagggggcg gttttcagag cgggcctgca gaatggcctg cacgtcggca
     3181 tcttccaaca ggcccgattc cagggccagc tcttcgttgg tgggctcgcg gcccaactgc
     3241 tggacgagtg aacgctgcac ccgcagcagc cgggtgatgg cttcaaacag gtggacgggg
     3301 atgcggatgg tacgggcctg ttcggcaata tagcggctga tggattgacg gatccaccag
     3361 gtggcatagg tgctgaattt gaagccgcgg gtggggtcaa acttggcaac ggcgcgcagc
     3421 aagcccaggt tgccctcttg aatcaggtcg aggaatgaga tgccgcgccc caggtagcgc
     3481 ttggcaatgc tgaccaccag ccgcaggttg gcgcggatga gggtctggtt ggcttcttca
     3541 gagcggaagg ccacgccttc ggcttcctta gccagatcga tatcgggcgg cagcaggcgg
     3601 tcaaagtctt ccatggtcag ttcaaaaggc ccgtggttga catctgacat caggccgact
     3661 gcggccgatt cgggcaacag gtagaggcac aggaagaggg tgaaggcatg gcgggccaca
     3721 tcatcccaga gcgggtcgcg gccccacagg ccgttatcca aaaagctgcg caggtaagag
     3781 ggggcatcca tctgccagga gaggcgtaac tgctgggctt cagagaggat cagggagaag
     3841 tcgggcaggt cgtggttggc caggcggccc gagtcttgca gcaggcgctc ccaggcgtta
     3901 atcaggtcct gggcaatggt cagcatcagg gcgcggtaat ggccgggctc gccgggcggc
     3961 ggcgaaacat gcccgctgag cgcctccagg cggtgctcgg cctcaatgcg cgcggccagg
     4021 cggaattcag agtcggcatc cagcaggtgg atgaggccaa tttctttcag gtagagtcgc
     4081 acggggtctt ctgaaagctc cacggcaatg gtggggtctc ccagaatttc cagggggtcg
     4141 tcaacgggga tctcagccca ctcttcagtc acgggcaggg ctggttcttc ggcttcagaa
     4201 acgccatttt caatttctgc tgacgcggct ggctggcgcg cggcattttt gctgctgcgc
     4261 gggcggccgc ggcgggcggg tgttttagcg ggggcagggg tgggtgtttt tttacgaacc
     4321 atgcgtcagt tcagatggag cttgaagggt tggtttgaac atgaatggtt aactatacca
     4381 tattttgccc ggcgg
//
LOCUS       DAOPVS010000160         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_20390_length_4337_cov_53.870388, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000160 DAOPVS010000000
VERSION     DAOPVS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20390_length_4337_cov_53.870388"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2022
                     /locus_tag="PKL11_09745"
     CDS             127..2022
                     /locus_tag="PKL11_09745"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNZ13925.1"
                     /translation="MKKITALLVSIILIMTLAACSVIPAATTSTTETSSQNAPEMAAT
                     SESATTASQPAAATDPASAIIYQDIHEDASDYVWDAAAVVPMTLDGNTITTSGAGVSI
                     NGSVANITAAGVYSLSGTLTDGQIVVNAPDDAVVQLILNGVNLNNSKGSPLVIEEADK
                     AILILAENTQNVISDSSAYVFASADTDEPNAAIFSKSDLTIYGSGALTVTGNYNDGIA
                     SKDGLLIASGTVSVTALDDGIRGKDYVAIKDGTITISSQGDGLKSDNEEETDRGFITI
                     AGGTITITSGADAVQAAKEVFVTSGALTLVTGGGSQSWTADTSSAKGIVATVGVTVDG
                     GTLSIDSADDALHSNGSITVNGGSLTLASGDDGLHADADLTINGGDIRVTRSYEGLEG
                     AVITVNAGNVLVTASDDGFNVAGGADGSGMGPGMLPPGGRPGGQDSFASVGSYYLYIN
                     GGYIFVDAGGDGLDSNGAIVMTGGTAIVNGPTENMNGALDYMAFTISGGTIIAVGSAG
                     MAQAAGSDSSSQYAVLVNYDSVQPAGTLIHIQNSAGEDILTFAPTRQYQSITFSSPAL
                     TNGETYTLYSGGSSTGTALNGLYQGGTYAPGSEVTTLTVSGLVTWSGAAGMGPGGGGP
                     GGRGGRP"
     gene            2177..3019
                     /locus_tag="PKL11_09750"
     CDS             2177..3019
                     /locus_tag="PKL11_09750"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNZ13926.1"
                     /translation="MKPTARLTETQNQSWQQSSLEWYSAPSQLAVITEGFAPISLKQM
                     DAVALLNRVDTKFVLTTAQLLQTLVALQSEYWVLAVKGHRLNHYRTLYFDTPNFDLYN
                     LHVNGRADRYKVRSREYTDSGDSFLEVKHKTRKDRTIKDRIPTSRPITHIPLEAEDWL
                     QAVFPFDSRKLEPKLWNTFTRITLVSKQCCERVTLDVDLTFYTPTRMARLDGLAIAEV
                     KKNLGHTGSPFLAQMRALRVRQQGFSKYCIGVSMLYDQVKKNTLKPKLLWLDRMVAGA
                     AYNE"
     gene            3012..3656
                     /locus_tag="PKL11_09755"
     CDS             3012..3656
                     /locus_tag="PKL11_09755"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNZ13927.1"
                     /translation="MSNFGNFILNFGFNFLVTLLVVRFIYYPSTHNKRYVFTFLAFNT
                     VIYFVLSFMTSIELGVGVGFGLFAIFSILRYRTDPIPIREMTYLFIISALPVMNSAGA
                     SGTIWPQLIAANLAIMAILWILEREWGFHYESSKRVIYEKIELIHPDHRAELIADLEA
                     RTGLKIKRVAIGKIDFVRDIADLKLFYSDPAQEDWLHNAEPDVVVVTSSSDVQQ"
     gene            3787..>4337
                     /locus_tag="PKL11_09760"
     CDS             3787..>4337
                     /locus_tag="PKL11_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ13928.1"
                     /translation="MTRKILVVDDTRNVQVLLIDFLSSQDFEVLSASDGREALEVVHQ
                     SDPDLILLDIMMPNMDGYQFITHLRRESNIPVIMITAKQQEADIIHGFDLGADDYITK
                     PFRLRELLVRMRAVLRRAAPRETAGSTLSIGDITLDGAKHEVKQASQVIDLTPLEFQI
                     LQILMQSPGQVVRRAELAMRLME"
BASE COUNT          976 a         1277 c         1084 g         1000 t
ORIGIN      
        1 aagcagcttt tgcgtcttaa ttgtcgctta taccaaattt ataccccttc acctggcctt
       61 ttcatacaat catgacgatt gaaaccgggg cgcataagcg tcctatctgt tcaaaggaga
      121 tattgtatga aaaaaataac agcattactg gtatcgatca ttctcatcat gaccctggcg
      181 gcctgttcag taattcccgc tgccaccacc agcacaactg aaacatccag ccaaaatgcg
      241 ccagagatgg cggcaacctc tgaaagcgca acaaccgctt ctcaaccggc ggctgccacc
      301 gatcctgctt ccgcgatcat ctatcaggat atacatgaag acgcttcaga ttatgtctgg
      361 gatgcggcgg cggtagttcc aatgaccctg gatggaaata ccatcaccac ctctggcgcc
      421 ggggtatcca tcaatggcag tgtagccaac atcactgctg cgggggtcta cagcctcagt
      481 ggcacgctca ctgatggaca gatcgtcgtg aacgcgcctg atgatgccgt tgttcaattg
      541 atccttaacg gagtcaatct gaacaactcc aaaggctcac ccttggtgat tgaagaagcc
      601 gacaaagcca tcctcatcct ggccgagaat acccaaaacg tgatttcgga cagctctgcc
      661 tatgtctttg ccagtgccga taccgatgaa cccaacgcag ccatcttcag caaatctgac
      721 ctgacaattt acggcagtgg agccctcacc gtcaccggta attacaatga tggcattgcc
      781 agcaaagacg gcctgctcat tgccagcggc acagtgtccg tcactgcttt ggatgatggt
      841 attcgcggca aggattatgt cgccatcaaa gacggaacca tcaccatttc atcacagggc
      901 gatggtctta aatcggataa cgaggaagaa accgaccggg gtttcatcac tatcgcgggg
      961 ggaaccatca ccatcacctc cggcgctgat gctgtccagg ccgccaaaga ggtctttgtc
     1021 acctctggcg cgctcaccct ggtcacaggc ggcggcagtc agagctggac ggcagacacc
     1081 tcttctgcca aaggcatagt tgccacggtt ggtgttacgg ttgacggcgg cacattatcc
     1141 attgattccg ccgatgacgc actccattct aacggcagca ttaccgtcaa tggcggttcg
     1201 ctgacccttg cctccggcga tgatggtctc cacgctgatg ccgacctgac cattaacggc
     1261 ggcgatatcc gcgttacccg gtcttacgaa ggcctggaag gcgccgttat cacggtcaat
     1321 gccggtaatg ttctcgtcac agccagtgac gacggcttca atgtggctgg gggcgccgat
     1381 ggctctggca tggggccggg catgctcccg cccggtggac gccccggcgg gcaggattct
     1441 ttcgccagtg ttggcagcta ctatctctac atcaacggcg gctatatatt tgtggatgcc
     1501 ggtggtgacg gactcgattc aaacggcgcc attgtaatga cgggcggcac tgcaatcgtc
     1561 aatggtccca ccgaaaacat gaatggcgca ctcgattata tggccttcac aatctcaggc
     1621 ggaacaatta tcgcggtcgg cagcgctggc atggcgcagg ccgccggcag cgactcttcg
     1681 agccagtacg ctgtcctggt gaactatgat agcgtccaac cggcaggaac tttgatccat
     1741 attcagaaca gcgctggtga agatatcctc acctttgctc ctaccaggca atatcagtcc
     1801 atcaccttct cctccccggc cctgaccaat ggtgaaacct ataccctcta ttcgggcggc
     1861 agttccaccg gcaccgcttt gaatggcctg taccagggcg gtacgtatgc ccccggctct
     1921 gaggtcacca cgctgaccgt ctcgggcctg gtcacctggt ctggtgctgc gggcatgggc
     1981 cctggcggcg gtggccctgg cggccgcggc gggcgcccgt aatccgaatt actccatgcg
     2041 agggctgttt ctgtctcctc caggggcggc cctcgccacc ctgcctatgg cctttataca
     2101 aaaaataaac cacttcattg gtcataacag gtaacatcaa gatagtccaa tgcactcgta
     2161 tccaaaggag atggatttga aacctacagc ccgccttacc gaaactcaga accaatcctg
     2221 gcagcagtcc tcccttgaat ggtattcggc cccgtctcaa ctggctgtca ttacagaagg
     2281 ctttgcgccc atcagcctca aacaaatgga cgccgtagcc ctgcttaacc gcgtggatac
     2341 caagtttgtc ctcaccacgg cgcaattgct gcaaaccctg gtcgccttgc agtcagaata
     2401 ctgggtgctg gcggtcaaag gccaccgcct gaatcactat cgcacccttt attttgatac
     2461 ccctaatttt gatctttaca acttgcacgt caatggccgc gctgatcgct ataaagtccg
     2521 cagccgtgaa tacaccgact cgggcgattc gttcctggaa gtgaaacaca aaacccgcaa
     2581 agaccgtaca atcaaagacc gcatccccac ttcgcgccca atcacgcaca ttccgcttga
     2641 agccgaagac tggctgcagg ctgtcttccc ttttgacagc cgtaagctgg aacccaagct
     2701 ctggaatacc tttacccgca tcaccctggt gagcaagcag tgctgcgagc gcgtcacgct
     2761 ggatgtggac ctcacctttt acacacctac ccggatggcg cgtttggatg gcctggcaat
     2821 tgccgaagtc aaaaagaatc tggggcatac cggctccccc ttcctggcac agatgcgtgc
     2881 cttgcgtgtg cgccagcagg gcttcagcaa gtactgcatt ggcgtttcca tgttatatga
     2941 ccaggttaag aaaaacactt tgaagcccaa attattgtgg ctggatagaa tggttgcagg
     3001 agctgcttac aatgagtaat tttggtaact ttattttgaa ctttggcttt aattttctgg
     3061 tgactttatt ggtggtgcgg tttatctatt acccatctac ccataacaaa cgctacgtct
     3121 tcacttttct ggcgtttaat accgtcatct attttgtact gagcttcatg accagcattg
     3181 aactgggcgt tggggtgggc tttggcctct ttgccatctt ctcaattctg cgctaccgca
     3241 cagatccaat tccgattcgt gagatgactt acctctttat catctccgcc ctccccgtga
     3301 tgaactccgc cggcgccagc ggtacaatct ggccccagtt gattgccgcc aacctggcaa
     3361 ttatggctat actgtggatt ctggagcgcg aatggggctt ccactatgaa tcttccaagc
     3421 gggtgattta tgagaagatc gaactcattc accctgacca ccgggctgaa ctgattgccg
     3481 atctggaagc ccgcaccggc cttaaaatca aacgcgttgc catcggcaaa atcgactttg
     3541 tccgggatat cgctgacctg aagctctttt atagcgatcc cgcccaggaa gactggctgc
     3601 ataacgccga acccgatgtg gttgtggtta ccagcagcag cgatgtccag caataatccg
     3661 ctgttctcct cctctgaccg cgcagccgga cggcccaggc acccccttcg gctgcgcccc
     3721 aatccggtcc aaaagcaagg ccacttgtgc cgtcaatgac ccgctgagat tgaatataat
     3781 aacgttatga cccgtaaaat attggttgtg gatgataccc gaaacgtgca ggtgctgttg
     3841 atcgacttcc tcagcagcca ggattttgag gtcctgagcg cctcggatgg ccgtgaagcg
     3901 ctggaggtcg tccatcagtc cgaccctgac ctgatcctgc tggatattat gatgcccaac
     3961 atggacggct accagtttat cacccacctg cgccgggaat caaacatccc cgtgatcatg
     4021 attaccgcca aacagcagga agccgacatc attcacggtt ttgacctggg tgccgatgat
     4081 tacatcacca agcccttccg cctgcgtgaa ctgctcgtgc gcatgcgggc ggtgctgcgc
     4141 cgggctgccc cgcgggaaac cgccggttcc acgttgtcta tcggcgatat taccctggac
     4201 ggcgccaaac acgaagtgaa acaggccagc caggttatag acctcacccc cctggaattt
     4261 caaattctcc aaatcttgat gcagtctcct ggtcaggttg tgcggcgcgc cgaactggcc
     4321 atgcgcctga tggagaa
//
LOCUS       DAOPVS010000161         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_20451_length_4327_cov_52.433989, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000161 DAOPVS010000000
VERSION     DAOPVS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20451_length_4327_cov_52.433989"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKL11_09765"
     CDS             <1..452
                     /locus_tag="PKL11_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNZ13929.1"
                     /translation="MLISDHIGLMGMTGPNPLCGPNLDTFGPRFPDMSQAYDHDLLAL
                     AQKVAAEVNIKVHTGVYIGLSGPSFETPADLRFLRVIGADAVGMSTVPEVTIARHGGT
                     RVLGISGISNKANLDGSTMTTHAEVLEAGKVIAPKMETIIRSVLRRI"
     gene            456..1145
                     /locus_tag="PKL11_09770"
     CDS             456..1145
                     /locus_tag="PKL11_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13930.1"
                     /translation="MADFLAFLQQYEIWIYLILGGVALVYLQKTLKASHDYRNALFGL
                     EREKARRVYRSALSVLLLSVLLAVANLALVSFVWPNLPASGLPTPTMDIGAQPTATLP
                     VAVEPQLTQTGVVIPTVAIAEGCLPGQIEWIFPTAGAEVQETVMLQGIVNVANLGFFK
                     YEYGQPGLDNWLPIAAGNAPTSTEAPDFGVWDTSQLIPGDYLLRLVVYDNQNNPFPAC
                     IVPVRVLAPPE"
     gene            1156..1695
                     /locus_tag="PKL11_09775"
     CDS             1156..1695
                     /locus_tag="PKL11_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13931.1"
                     /translation="MPQIEIRPAVAEDIPLLVALDHDYETTQVWRLEPLTEDDLTAVG
                     FREVRLPRPMRVAYPRTPQNLVNTWQRHDLMIVAVLSGEPVGYLTLSDRQAPNAAWVT
                     DLVIAPSVRRQGIGSALLLASQGWAAQRNLRCMLAEMQTKNAPMIRMAQKLGYTFSGH
                     HEHYYANQETGLFFTRFLK"
     gene            1805..>2654
                     /locus_tag="PKL11_09780"
     CDS             1805..>2654
                     /locus_tag="PKL11_09780"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNZ13932.1"
                     /translation="MAFSLLLYALTFNLREGVVRAFAVILACIVVAFTAEAIGSTAIE
                     AWQIEFWLRLQWVGIILLPAAYFQFSDALLATTGKPSRRRKWILALSYLASLAFLISL
                     AMSWLIGPLITSGPPAPHLQPTLLTDIFVVYYLAMMAFSAFNFIRAYRRTVTPTSRRR
                     MGYLLIGAVAPPIGSFPFLLFGSDFAAFHPIVFWWLALLTNLLLGAMIIIMAYAVAFF
                     DAFWSDRVVKARLLKWIMRGPVTASLVLALVTIVRRIGETFGFSYTALVPIIMVATIL
                     IAEHFIS"
     assembly_gap    2655..2754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2755..3813
                     /locus_tag="PKL11_09785"
     CDS             <2755..3813
                     /locus_tag="PKL11_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13933.1"
                     /translation="LVPIIMVATILIAEHFISTFAPVAERALFWDGDAQDLQMLRLLE
                     GRLLTRNDLQQFLEMILAVVCDRLQAPGAYIAGFNGEGLERIVTIGRTRFQQESASNL
                     LEAISEEDTIHQGTFRWGNDLLIPLQATTEDGSVALLGILGISESAALVLDDEPRRQL
                     SLLARRAALALRDRQMQTQVFSALQALAPQVEFIQRLRAAGRYDRADILSNEDLPYTP
                     DDLSLWVRDALTHYWGGPRLTESPLLQLQLVQNALDAHDGNPTNALRSILRDAIDRVR
                     PEGERRFTGEWILYNILEMKFLEGKKVREIAARLAMSEADLYRKQRVAIEAVAQAILD
                     MEAQARRENGEGQGEILD"
     gene            3876..>4327
                     /locus_tag="PKL11_09790"
     CDS             3876..>4327
                     /locus_tag="PKL11_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNZ13934.1"
                     /translation="MPKAQGNETPGPENLDEGWWTALLSEDNLANNRVRGVALSAAPL
                     SESVNWATVQRAYAADQIVCLTVHGYNRGGLLVQADSVQGFVPLSHLVDVPNTASETE
                     RRELLAAYVGRVLQLKVIECEPEQERVIFSERAAQAGEGRRKMLFESL"
BASE COUNT          816 a         1274 c         1111 g         1026 t
ORIGIN      
        1 tgatgctcat ctccgatcat atcggtctga tgggaatgac tggcccgaac ccgctttgcg
       61 gccccaatct ggacaccttc ggcccgcgct tccctgatat gagccaggcc tatgaccacg
      121 atttgttagc cctggcccaa aaagttgcgg ctgaagtaaa tatcaaggtc cacacgggtg
      181 tctatatcgg ccttagcggc ccctcttttg aaacccccgc cgatctgcgt ttcctgcgcg
      241 tgattggcgc cgatgccgtt ggcatgtcca ccgtccccga agtaaccatc gcccgccatg
      301 gcggcactcg ggttctgggc atttcgggca tcagcaacaa ggccaacctg gatggcagca
      361 ccatgaccac ccacgccgaa gtgctggaag ccggcaaagt gattgccccc aaaatggaga
      421 ccatcattcg cagcgtcctg cgtcgcatct aatttatggc agactttctg gcttttttgc
      481 agcaatacga aatctggatc tacctcatcc tgggcggggt agccctggtt tatttgcaaa
      541 aaacgcttaa agcctcccac gattatcgca atgcgctctt tggcctggaa cgcgaaaaag
      601 cccgccgggt ctatcgcagc gcattatctg tgttgctgct gtctgtcttg ctggcggtgg
      661 ctaatttggc gcttgtgtct tttgtctggc ccaacctgcc ggcgtcaggc ctgccaaccc
      721 ccaccatgga tatcggcgca cagcccacgg ccacgctgcc ggttgctgtt gagcctcaac
      781 tgacccagac aggcgtcgtt atccccacgg ttgcaattgc cgagggctgc ctgcccggcc
      841 agattgaatg gattttcccc accgctggcg ccgaggtgca ggaaaccgtc atgcttcagg
      901 gcatcgtcaa tgtggccaac ctgggcttct tcaaatatga atacggccag cccggactgg
      961 ataactggct gcccattgca gcaggtaacg cccccacctc gaccgaagcc cctgactttg
     1021 gcgtctggga cacttcccag cttatccccg gtgactatct tttacgtctg gttgtctatg
     1081 acaatcagaa caatcccttc ccggcgtgca tcgttccggt tcgcgtcctt gcaccccccg
     1141 aataacggag ctagtatgcc tcaaattgaa atccgcccgg ctgtagccga agatatcccc
     1201 ttactggtag cccttgatca tgactatgaa accacccagg tctggcgcct ggaacccctg
     1261 accgaggatg atcttactgc cgtgggcttc cgtgaggttc gcctgccgcg cccgatgcgt
     1321 gtagcttatc cgcgtacgcc gcaaaacctg gtcaacacct ggcagcgcca cgatttgatg
     1381 atcgttgccg ttctatcggg tgagccagtg ggctatctga cactcagtga tcgtcaggcg
     1441 ccaaacgccg cctgggtaac tgacctggtg attgccccct cagtccggcg gcagggcatt
     1501 ggcagcgcct tgttgctggc ttctcaaggc tgggcggcgc agcgcaacct gcgctgcatg
     1561 ttggcagaaa tgcagaccaa aaacgcgccc atgatccgca tggcgcaaaa actgggctat
     1621 accttcagcg gccatcacga acattattat gccaatcaag aaactggcct cttctttacc
     1681 cgttttttga aatgagtttg tcgtttttat tggaaagggc gcaattgtga tcagcggctg
     1741 gcaatcaggc gggcttaccg cccttatcac catcagccag attctgacgg ccggaattgc
     1801 cattatggca ttttcgctgt tgctttacgc cctcaccttc aatctccgcg aaggcgttgt
     1861 ccgcgcgttt gcggtcattc tggcctgtat cgttgtcgcc ttcaccgctg aagccatcgg
     1921 cagcacggcc atcgaggcct ggcagattga attctggctc cgcctgcagt gggttggcat
     1981 catcctgctt ccggcagctt attttcaatt ctccgatgcc ctgcttgcca caaccggcaa
     2041 acccagccgc cgccgcaaat ggattctggc gctgtcctac ctggcctcac tggcatttct
     2101 gatctccctg gccatgtcct ggctgattgg cccattgatc acctccggcc cgccagcgcc
     2161 ccacctgcag cccaccttgc tgaccgacat ttttgtggtc tattatctgg ccatgatggc
     2221 cttctcggcc ttcaacttta tccgcgccta ccgtcgcaca gtcacgccca ccagccgccg
     2281 ccggatgggc tatttgttga tcggcgccgt ggcaccgccg attggttcat tccccttcct
     2341 gctgttcggc tcagattttg ccgcttttca cccaatcgtc ttctggtggc tggctttact
     2401 gaccaacctc cttttaggcg ccatgattat tattatggcg tatgccgtcg ccttttttga
     2461 tgccttctgg tcagaccggg tggtcaaggc tcgtctgctc aagtggatta tgcgcggccc
     2521 ggtaaccgcc agtctggtct tggccctggt aactattgtg cgccgcattg gcgaaacctt
     2581 tggcttcagt tacaccgccc tggtgcccat catcatggta gccaccattc tgatcgctga
     2641 gcatttcatt tccannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctggtg
     2761 cccatcatca tggtagccac cattctgatc gctgagcatt tcatttccac ttttgcgccg
     2821 gtggcagaac gggcgctctt ttgggatggc gacgcccagg atctccagat gctgcgcctg
     2881 ctggaaggcc gcctcctgac gcgcaatgac ctccagcagt ttttagaaat gatcctggct
     2941 gtggtttgtg accgccttca ggcgcctggc gcctatattg ccggcttcaa tggcgagggc
     3001 cttgaacgga ttgtgacgat tggccgcacc cgctttcagc aggaatcggc ctccaacctg
     3061 ctggaagcca tctccgagga agataccatc catcagggca cttttcgctg gggcaatgac
     3121 ctactgatcc cgctgcaagc taccactgaa gatggctcgg tagccctgct tggcattctg
     3181 ggcattagcg agtccgctgc tctggttttg gatgatgaac cccggcggca gctttctctg
     3241 ctggcgcgcc gcgcagctct tgccttacgc gaccgccaga tgcaaaccca ggtcttcagt
     3301 gccttacagg cccttgctcc ccaggttgaa tttatccagc gcttgcgcgc tgccggtcgc
     3361 tatgaccgcg ccgacatctt gtccaacgaa gatttaccct atacccccga cgatctatct
     3421 ttgtgggttc gtgacgcgct cacacattat tggggcggtc cccgcctgac ggaaagcccg
     3481 ctcttacagc ttcaactggt tcaaaacgcc ctggatgctc acgacggtaa ccccaccaat
     3541 gccctgcgtt cgattctacg tgatgccatt gaccgcgttc gtccagaagg cgaacgacgc
     3601 ttcacaggcg agtggatatt gtataatatc ttggaaatga aattcctgga aggcaaaaaa
     3661 gtacgtgaaa tagctgcgcg gctagccatg tctgaggccg atttgtatcg caaacagcgc
     3721 gttgcgattg aagcagtggc tcaggctatt ttagatatgg aagcccaggc gcggcgtgaa
     3781 aacggggagg gccagggaga aatattggat tgatctctgg atattgttaa taatgcactt
     3841 attgatcatt gttgtacgga gggaacatgg tacccatgcc aaaggctcag ggaaacgaaa
     3901 ccccgggccc tgaaaacttg gatgagggct ggtggacagc actattatct gaagataatc
     3961 ttgccaataa ccgcgtccgt ggggttgcgc tgtcagccgc ccctttatca gaatcggtca
     4021 actgggcaac agtccagcga gcctatgctg ccgatcaaat cgtctgcctg acagttcatg
     4081 gttacaaccg gggtgggttg cttgttcagg ccgatagcgt tcagggcttt gtgccgctgt
     4141 cccatttggt agatgtgccc aacaccgcca gcgaaaccga gcgccgtgaa ttgctggcgg
     4201 cttatgtggg gcgcgtctta cagctcaaag tcattgaatg tgaacccgaa caggaacgcg
     4261 tcatcttctc tgagcgcgcc gctcaggccg gcgaaggccg ccgcaaaatg ctctttgaat
     4321 ctttgca
//
LOCUS       DAOPVS010000162         4284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_20715_length_4284_cov_59.259636, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000162 DAOPVS010000000
VERSION     DAOPVS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20715_length_4284_cov_59.259636"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PKL11_09795"
     CDS             complement(<1..1271)
                     /locus_tag="PKL11_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNZ13935.1"
                     /translation="MMGKIQSIVKRTGAVVPFNAERITNAIYRAAIAVGGRDRSTAEA
                     LTAEVVQALENSIAPDHIPSVEEIQDMVEKVLIENGHAKVAKAYILYRDERARDRQER
                     AKLAARPSETIPWAKIWHVLDWAVSQDLHTIAGLNARIERGELPQIVEACETAYNQDV
                     ENAAEMIALRRDKVKMVIITGPSSSGKTTTTIKIGQRLAYLGLKLVTLNLDNYFYDLA
                     LQPKDEFGDYDFETPQALDLKLINEHLSRLTAGEEIMMPYYDFKQGKRYDDRTPMRLG
                     PNEIVLIDSLHGLYPEMTQAVPHDQKFKVYLEPLMQLKDSQGQYVRWTDIRLMRRMLR
                     DAAHRGYEPRQTLVHWHYVRTSEKRNIIPFLNTADIIVNSAMPHELCIYKPRLMTHFA
                     GWTEEFKGDPLREDAYTRAVRVNAMLAEITPL"
     gene            1709..>4284
                     /gene="ileS"
                     /locus_tag="PKL11_09800"
     CDS             1709..>4284
                     /gene="ileS"
                     /locus_tag="PKL11_09800"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNZ13936.1"
                     /translation="MFKPVSPRLNITLLEEAMLRFWKQHNIFQETMTQREGGPEYVFF
                     EGPPTANGRPGIHHVLARAFKDIFPRYKTMRGYHVSRRGGWDTHGLPVELEVEKKLGF
                     TNKQQIEAYGIGKFNALCRESAFTYIQEWERMTDRMGYWVDMQSAYVTFRDSYIESVW
                     WILKNFWEQDLLYQGFKVVAYCPRCGTPLSDHEVAQGYADATDPSVYVRLPLVDQPGV
                     SLLVWTTTPWTLPANVAVAAHPDIEYVMVEREMPEGGTERLILAEALLTKVFDQPEKL
                     RIVERFKGKHLKGKQYRPLFTFLPPDKPAHYVVLANYVTTEDGTGLVHIAPAFGADDM
                     NVSNEYDLPVLMTVAEDGTFIPAVRPWAGVFVKDADPAITEDLNRRGLLFKSGSYTHT
                     YPFCWRCDTPLLYYARPTWYIRTSQFKDRMVALNNDINWYPGHIKAGRFGNWLSNNVD
                     WALGRERYWGTPLPVWECGDCHHQECIGSVSELSQKAGRDLSNLDLHRPHVDEIHWRC
                     AECGGTMQRVPELIDVWFDSGAMPVAQWHYPFENLEAFKQQFPADFICEAVDQTRGWF
                     YTLHAISSLLFDSVAFKNVICLGLILDAEGRKMSKHKGNVVNPWDVLNEYGADATRWY
                     LYTASPSGQERRFSMDLVGEVVRTFTLTLWNTYSFFVTYANLDGWRPDPAVQPEYSPL
                     DRWVRSALHTLVRDVTDALENYDVLGATRPITTFVDQLSNWWLRRSRRRFWKSEADAD
                     KQAAYSTLYEALTTLSRLLAPTMPFLAEELHQNLVRTMDESAPLSIHLAPWPAFDPAV
                     VDEKLNREMALVMRLASLGHAARNKANRKVRQPLAEVAFSVANIEEAGVVLRYAELLA
                     DEL"
BASE COUNT          873 a         1330 c         1111 g          970 t
ORIGIN      
        1 agcggcgtga tctctgcaag catggcgttc acccgtacgg cgcgggtata agcgtcctcg
       61 cgcagcgggt cgcctttgaa ttcttccgtc cagccggcaa aatgcgtcat cagccgcggc
      121 ttgtagatgc acagctcatg cggcatggcg ctgttcacaa taatatctgc cgtgttgagg
      181 aaggggataa tattccgctt ctcgcttgtg cgcacataat gccagtgcac cagggtttgc
      241 cgcggctcgt agccccggtg ggctgcgtcg cgcagcatcc gacgcatcag tcggatatcc
      301 gtccagcgca catactggcc ttgtgaatcc ttcaactgca tcagcggctc aaggtacacc
      361 ttgaattttt ggtcgtgtgg cactgcctgc gtcatctccg gatacaaccc gtgcaggctg
      421 tcaatcagca caatctcgtt gggtcccagc cgcatggggg tccggtcgtc ataccgtttc
      481 ccctgtttga aatcgtaata cggcatcatg atctcttcgc cagcggtcag gcggctcaga
      541 tgttcgttga tcagcttcaa atccagcgcc tggggcgtct caaaatcgta gtccccaaac
      601 tcatccttgg gctgcagcgc cagatcatag aaataattgt ccagattcag cgttaccagt
      661 ttcagcccca ggtaggccag ccgctgacca attttgatgg tggtcgttgt tttccccgat
      721 gacgaaggcc cggtaataat taccatcttc accttgtccc ggcgcagggc aatcatctca
      781 gccgcatttt ccacatcctg gttataggct gtttcgcagg cctccacaat ttgcggcagt
      841 tcgccacgct caatccgcgc gttcaatcct gcaatcgtat gcagatcctg gctcaccgcc
      901 cagtccagca catgccagat cttcgcccag gggatcgtct cggaaggtcg ggcagccagc
      961 ttggcgcgtt cctgccggtc ccgggcgcgt tcatcacggt agagaatata ggccttcgcc
     1021 accttggcat gaccattctc aatcaatacc ttttccacca tatcttgaat ttcttccacc
     1081 gatggaatat ggtctggggc aatgctgttt tccagcgcct gaacaacctc cgccgtcagc
     1141 gcctcagcgg tactgcggtc gcgtccgcct actgcaattg ccgcccgata aatggcattg
     1201 gtgatccgtt ctgcattaaa tggcacaact gcgccggttc gtttaacaat gctctgaatt
     1261 tttcccatca ttgcacctca aagacgaaag aacatcattg gataggtttt aattatagca
     1321 tgtctgacaa taccccaaat gccgctaaag ccgcgtttca attctaaaaa ccccatttgc
     1381 attctggctg gaattgtggc ataattcaac catcaacagc gttgatggaa acgagtaaat
     1441 ccgccccgcg ctgtcagcga acccggatca aggtgcaagc cgggccagcc cagcggattg
     1501 aaaatccttc cggagctgca aactgaacgt ggatctccac cagtaggtgc gccggcctcg
     1561 ccagccgtta tcctgggcgc agatattatc cgccaaagga cgtatctgac cgagcgctca
     1621 ctgcgaggtg agaagcaggg tggtaccgcg agaaagccct ctcgtccctg tgggatgcga
     1681 gggcttttta attggtcaag gaggccttat gtttaaacct gtttcacccc gcttgaatat
     1741 cactctgttg gaagaggcca tgctgcgctt ctggaagcag cacaatatct tccaggagac
     1801 catgacccag cgcgaaggcg gccccgaata tgtcttcttc gaaggcccgc ctacggctaa
     1861 cggccgcccg ggcattcacc atgtgctggc ccgggcattc aaagacatct tcccgcgcta
     1921 taagaccatg cgcggctacc atgtcagccg ccgcggcggt tgggataccc acggcctgcc
     1981 cgttgagctg gaagttgaaa agaagctcgg cttcaccaac aaacagcaaa ttgaagccta
     2041 cggcattggc aagtttaacg ccctctgccg cgagtcggcc ttcacctaca tccaggaatg
     2101 ggaacgcatg accgatcgga tgggttactg ggtggatatg cagtctgcct atgtgacctt
     2161 ccgcgattcc tacatcgaat cagtctggtg gatcctcaaa aatttctggg agcaagacct
     2221 gctctatcag ggtttcaaag tggttgccta ctgcccgcgc tgcggcaccc cgctgtctga
     2281 ccacgaagtt gcccagggct atgccgatgc cactgaccca tcggtctatg tccgcctgcc
     2341 tctggtcgat cagcccggcg tttcgctcct cgtctggacc accacaccct ggactctgcc
     2401 tgccaacgtc gccgttgcgg cccacccgga catcgaatac gtcatggtcg agcgcgaaat
     2461 gcccgaaggc ggcaccgagc ggctcatcct ggcagaagcc ctgctgacca aagtctttga
     2521 tcagcccgag aaactgcgca tagtcgagag atttaagggc aagcacctca aaggcaagca
     2581 gtatcgcccg ctgttcacct tcctgccgcc cgacaaaccg gcgcattacg tcgtcctcgc
     2641 gaattacgtc accacggaag atggcaccgg gttggtccat attgctccgg cattcggcgc
     2701 tgatgacatg aatgtttcca acgaatacga cctgcctgtg ctgatgacag ttgccgaaga
     2761 tggcactttc atccctgcgg tgcgcccctg ggcgggggtg tttgtcaaag atgctgaccc
     2821 tgccatcact gaagacctca accgccgcgg cctgctcttc aaatctggct cctatacaca
     2881 tacctatcct ttctgctggc gttgcgatac gccgctgctt tactatgccc gcccaacctg
     2941 gtacatccgc accagccagt tcaaagaccg catggttgct ctcaacaatg acatcaactg
     3001 gtatcccggt cacatcaaag ctggtcgttt tggcaactgg ctgtccaata atgtggactg
     3061 ggcgctgggg cgtgaacgct actggggcac cccgctgccc gtctgggaat gtggcgactg
     3121 ccatcaccag gagtgcattg gttctgtgtc cgaactgtcg caaaaagcag gccgtgacct
     3181 cagcaacctc gatctgcatc gcccgcatgt ggatgagatc cactggcgct gtgccgaatg
     3241 tggcggcacc atgcagcgcg tccccgaact gatcgatgtc tggttcgatt caggcgccat
     3301 gccggttgcc cagtggcact acccctttga aaacctcgaa gccttcaaac aacaattccc
     3361 cgctgatttt atctgcgagg ccgtggatca aacccggggt tggttctaca ccctgcacgc
     3421 catctcctcg cttttgttcg acagcgtggc cttcaagaat gtcatctgcc tgggcctgat
     3481 cctcgatgct gaaggccgca agatgtccaa gcacaagggc aatgtcgtca acccctggga
     3541 tgtgctgaac gaatacggtg ccgacgccac tcgctggtat ctctacaccg cttcaccctc
     3601 gggtcaggag cgccgtttct ccatggatct ggtgggcgag gtcgtccgca ccttcacctt
     3661 gacgctgtgg aatacctatt ccttcttcgt cacctatgcc aacctggatg gctggcgtcc
     3721 ggaccccgca gtccagcctg aatacagccc gctggatcgc tgggtgcgct ccgccctgca
     3781 taccctggtg cgtgatgtga ccgatgccct ggaaaactat gatgtcctgg gcgccacccg
     3841 tcccatcacc acctttgttg atcaactctc caactggtgg ctgcgccgct cgcgccgccg
     3901 cttctggaag agcgaagccg atgccgataa gcaggccgcc tattccacgc tctatgaagc
     3961 cctgaccacc ctcagccgct tgctggcgcc caccatgccc ttcctggctg aagaactgca
     4021 ccagaatctc gtccgcacca tggatgaatc tgcgccgctc tctatccacc tggccccctg
     4081 gcctgcgttt gatccggcag ttgttgacga gaaactgaac cgggagatgg ccctggtcat
     4141 gcgcctggct tcgctgggcc atgctgcccg caacaaagcc aatcgcaaag ttcgccagcc
     4201 ccttgctgaa gttgccttct ccgtggcaaa tatcgaagaa gcaggtgtgg tcttgcgtta
     4261 tgccgaactg ctcgctgatg agct
//
LOCUS       DAOPVS010000163         4259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_20899_length_4259_cov_62.212655, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000163 DAOPVS010000000
VERSION     DAOPVS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20899_length_4259_cov_62.212655"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1358
                     /locus_tag="PKL11_09805"
     CDS             186..1358
                     /locus_tag="PKL11_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ13937.1"
                     /translation="MGKNLSIASYLAVKEVWRNRGRFLLVSLVIALITLLVLFIAALG
                     EGLGNGNREYISKLDAEVIVFQAKSDYLIAASRLGRDRLAAVRRVEGVADAGMLGTAS
                     ANLILPEDGETLKVAILGVEPGRVGEPQVVEGQQLSTDLAEEAIIDSNTAIRSGLGLG
                     DTLTLRVTQGTQDEFFSLEIVGITDGQQYSLQPSVFVPFFTWDRIRPKSEAEVNSSNP
                     IANVILVKLEDPAQLEAVSQRLTAQVNNVEAATLKTTIQSLPGYSAQQSTLQTQGGFT
                     LFIGILVIGGFFQIQILQKVAQIGVLKAIGTANPIVAMSSIIQIVVITIIGVAIGGLL
                     TFLFSLTFPPTVPIVFNGTTTAIAIAALLLIGPLGGLVSIRYAVRIEPLKALGLSS"
     gene            1370..2098
                     /locus_tag="PKL11_09810"
     CDS             1370..2098
                     /locus_tag="PKL11_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13938.1"
                     /translation="MTTYALETQSVIKTYTSESGTINAVDDVSLQVAAGEFVALVGPS
                     GSGKTTMLSILAALLQPSSGKILLDGDDLAGMSDLERVDMRREKIGFTFQANNLVPYL
                     TAVENVELMLRLNNKLDKAGKLRARELLARLGLGERLHNLPGQMSGGQQQRVAIARAL
                     IHNPSLVLADEPTASLDTERAYQVVETFAGLIHEQKRAGIMVTHDLRMCEFVDRVLLM
                     RDGKLVQIYAERDEIMDLARGGRH"
     gene            complement(2165..3157)
                     /locus_tag="PKL11_09815"
     CDS             complement(2165..3157)
                     /locus_tag="PKL11_09815"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNZ13939.1"
                     /translation="MTTNSTVLLRVRNLKKYFPIYSGLVLSRHVNDVKAVDDVSFDVY
                     RGETLGLVGETGCGKTTVGRTILRLYEPTAGQIVFDGTDINALGENELRAFRRRMQMI
                     FQDPYASLNPRMTVGSIISAPLDVHTKLSKKEKQDRVQELLKIVGLNPDFVNRYPHEF
                     SGGQRQRIGIARSLAINPELIICDEPISSLDVSIQAQVVNLLEELQDKLGLTYIFIAH
                     DLSMVRHISDRMAVMYLGKVMELADRNEIYLRPMHPYTQALMSAVPVPDPSRARNRQR
                     ILLEGDIPSPVNPPVGCHFHTRCPWADEHCRQVDPEYREIVPGHFVACHYARIN"
     gene            complement(3157..4149)
                     /locus_tag="PKL11_09820"
     CDS             complement(3157..4149)
                     /locus_tag="PKL11_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ13940.1"
                     /translation="MESLLTVKNLQTNFYTEDGVVRAVNGISYSMRPGEILGVVGESG
                     CGKSVHAMSILRLIQSPPGKIEGGEVWFNGRDLLQLTEDEMRKVRGHEIAMVFQDPMS
                     SLNPVYTVGFQISEALMLHKGMDKKAARKRAQELLVLVGIPEASKRLDNYPHQFSGGM
                     RQRAMIAMALSCEPKLLIADEPTTALDVTIQAQIINLVIKLQQELGMAVMWITHDLGV
                     VAEMVNTINVMYAGYIIERGPVMDIYKNPTHPYTVGLLGSLPRLDEAPGTKLASIPGL
                     PPDLLALPKGCPFYARCVYRAAQCTESMPPLEDTGTAGHIVACWRWKEIFRKGA"
BASE COUNT          905 a         1081 c         1360 g          913 t
ORIGIN      
        1 gtgttagacg cactgcccaa cgtaacgctg gatggcacag tcagccagat taaccaacgc
       61 tatgaagaaa agcgcggcga tattacctac accgtgacga ttaccctgac taaaactgat
      121 ccacaaatgc gctggggcat gacagccgcg gcgcggttcc tgccataagg cagcgggagc
      181 attctatggg aaagaattta tccattgcga gttacctggc tgtcaaggaa gtctggcgga
      241 atcgcgggcg cttcctgctg gtgagcctgg tcatcgcgct gattacgctg ctggtgttat
      301 tcattgcagc gctgggcgaa ggcctgggca acggcaaccg ggaatatatc tccaagctgg
      361 atgcggaagt gatcgtcttt caggcgaaat cggattacct gattgccgcc agccggctgg
      421 ggcgtgatcg tctggcagcc gtgcggcggg tagagggcgt tgccgacgcg ggtatgctgg
      481 ggactgccag cgccaatctg atcttgcccg aggatggcga gaccttgaag gttgccattc
      541 tgggcgtgga acccggacgt gtgggcgagc cgcaggtggt cgaagggcag cagctcagca
      601 cggatctggc tgaagaagcg attattgaca gcaacacggc catccgcagc gggctggggc
      661 tgggcgatac cttgaccctg cgggtgacgc agggtacgca ggatgagttt ttcagtttgg
      721 agatcgtagg catcaccgac gggcagcagt attctttgca gccgtctgtt tttgtacctt
      781 tctttacctg ggatcgcatt cgccccaagt ctgaggcgga agtgaacagc tcaaacccaa
      841 ttgcgaatgt catcctggtg aagctggaag atccggcgca gcttgaggcg gtgagccagc
      901 ggctgacagc gcaagtgaac aacgtggaag cagcaaccct taaaacgacc atccagtctt
      961 tgccggggta ttctgcacag caaagcaccc tgcaaaccca gggcggtttt accctgttca
     1021 ttggtattct ggtcatcggc gggttcttcc agattcagat tctgcagaag gtggctcaga
     1081 ttggggtgct gaaagccatc ggcacggcca atccgattgt ggcaatgtca tcgatcattc
     1141 aaatcgtggt cattaccatc attggcgtgg caattggcgg gctgctgacg tttttattct
     1201 cgctgacctt cccgcccacc gtgccaatcg tattcaacgg cacgacaacg gcgattgcca
     1261 tcgcagccct gctgttgatt ggcccgctgg gtgggctggt atccatccga tatgctgtgc
     1321 gcattgaacc gctcaaggcg ctcgggttgt cttcataatg ggtgacacaa tgactacata
     1381 tgcacttgaa acccaatcgg tgattaagac ctacacgagc gaatccggga cgattaatgc
     1441 agtggatgat gtctctttgc aggtggcggc tggcgagttt gtggcgctgg tgggaccgag
     1501 cggatctggc aaaaccacca tgctctcgat tctggcggcg ctgctgcagc ccagttcagg
     1561 taaaatcctg ctggatggcg atgatctggc gggaatgagc gacctggaac gcgtggacat
     1621 gcgccgggaa aagatcgggt tcacgtttca ggccaataac ctggtgccct atctgacggc
     1681 ggttgaaaat gtagaattga tgctgcgcct gaacaataag ctggataagg ctggaaagct
     1741 gcgggcgcgt gaactgctgg ctcgtctggg gctgggtgag cggctgcaca acctgccggg
     1801 gcagatgtcg ggcggacagc agcagcgggt ggcgattgca cgggcgctga ttcacaaccc
     1861 cagcctggtg ctggcagatg agccaaccgc cagcctggat acagagcgcg cctatcaggt
     1921 agtggaaacc tttgccggat tgatccacga gcagaagcgg gctggcatta tggtgacgca
     1981 tgatctgcgg atgtgcgagt ttgtggaccg tgtgctgctg atgcgagacg gcaagctggt
     2041 gcagatttat gccgagcgag acgagattat ggacctggcc cgaggtgggc ggcactagcc
     2101 ctgtcgggaa atcaaaaacg ccagcctctc agggctggcg ttttgtattt atagtatggt
     2161 ggcactaatt gatgcgcgca tagtggcagg ccacaaaatg gccggggaca atctcgcggt
     2221 attccgggtc cacctggcgg cagtgctcat cagcccaggg gcagcgggtg tggaagtggc
     2281 agcccaccgg ggggttgacc gggctgggga tatcgccttc cagcaggatg cgctggcggt
     2341 tgcgggcccg agagggatcg gggacgggga cggcagacat cagcgcctgg gtgtaaggat
     2401 gcatggggcg gaggtagatt tcgttgcgat cagccagttc catgaccttg cccagataca
     2461 tcacagccat gcggtcgctg atgtggcgaa ccatgctgag atcgtgggcg atgaagatat
     2521 aggtgaggcc gagtttgtcc tggagttctt ccagcagatt gaccacctgg gcctgaatgg
     2581 agacatccag cgaggaaatg ggttcatcac agatgatcag ctcggggttg atggccaggg
     2641 agcgggcaat gccgatgcgc tggcgctgac cgccagaaaa ttcgtgcggg taacgattga
     2701 caaaatcggg gttgaggccg acgattttca gcagttcctg aacccgatcc tgtttttctt
     2761 ttttagacag cttggtatgc acatccagcg gggctgaaat gatggagccc accgtcatgc
     2821 gagggttcag ggaagcgtaa gggtcctgga agatcatctg catccgacgg cggaaggcgc
     2881 gcagttcatt ttcgcccagg gcgttgatat cggtgccatc gaagacaatt tgcccggcgg
     2941 tgggctcata gaggcgcaag atggtgcgcc cgacggtggt cttgccacag ccggtttcac
     3001 caaccagacc aagggtctca ccgcgataga catcgaagct gacatcatcc acggctttga
     3061 catcatttac atggcggctg agcaccaatc cgctgtaaat tggaaagtat tttttcaggt
     3121 tgcgcacccg cagcaaaaca gttgagttcg ttgtcatcag gcacctttcc taaagatttc
     3181 tttccaacgc cagcaggcca caatgtgacc agcggtgccg gtgtcttcaa gcgggggcat
     3241 ggattcagta cactgggcgg cgcgatagac gcagcgggcg tagaagggac agcctttggg
     3301 aagcgccagc agatcgggcg gcaggcctgg aatggaggcc agtttcgtgc cgggggcttc
     3361 atccagacgg ggcagggaac ccagcagtcc aaccgtgtag ggatgggtgg ggtttttgta
     3421 gatgtccatg accgggccac gctcaatgat gtaaccagca tacatcacgt tgatggtatt
     3481 gaccatctcg gcaaccacgc cgaggtcgtg agtaatccac atgactgcca tgcccagttc
     3541 ttgttgcagc ttgataacca gattgatgat ctgcgcctgg atggtgacat ccaacgcggt
     3601 ggtgggttca tcggcgatca gcagtttggg ttcgcaggag agcgccatgg cgatcatggc
     3661 gcgctggcgc atgccgcccg agaattgatg ggggtagtta tccaggcgct tgctggcttc
     3721 agggatgccc accagcacca gcagttcctg ggcgcgtttg cgagccgcct ttttatccat
     3781 gcctttgtgg agcatgaggg cttcgctgat ctggaaaccg accgtataga ccgggttcag
     3841 agaggacatg gggtcctgga agaccatggc aatttcatga ccgcgcactt tgcgcatttc
     3901 atcttcagtc agttgaagca ggtcacgccc attgaaccag acctcaccgc cttcaatttt
     3961 cccggggggg gattggatga ggcggaggat ggacatggca tggacgctct tgccgcagcc
     4021 gctctcgccg accacaccca ggatttcacc ggggcgcatg gaataggaaa tgccattgac
     4081 agctcttacc acgccgtcct cggtgtagaa attggtttgc aggtttttga ccgttaacaa
     4141 agattccatg atgggtttct ccgtggggta gggggttaag tgcgcatctt gggatcgagg
     4201 gcatcccgca ggccgtcacc caggaggttg aagcccagga cactcaacat gatggccag
//
LOCUS       DAOPVS010000164         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_21389_length_4190_cov_68.127449, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000164 DAOPVS010000000
VERSION     DAOPVS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21389_length_4190_cov_68.127449"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..130)
                     /locus_tag="PKL11_09825"
     tRNA            complement(39..130)
                     /locus_tag="PKL11_09825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Ser,seq:gct)
     gene            complement(157..241)
                     /locus_tag="PKL11_09830"
     tRNA            complement(157..241)
                     /locus_tag="PKL11_09830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Ser,seq:tga)
     gene            complement(302..1651)
                     /locus_tag="PKL11_09835"
     CDS             complement(302..1651)
                     /locus_tag="PKL11_09835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ13941.1"
                     /translation="MSTPIPAELSLPRPLPDGLILRRSSTADADQLAEFNQRIHTADD
                     EEPDAHDSLAAWTRDLLNGQHPTHAPDDFTLVVEESTGKIVSAMNLISQTWAFDGLSL
                     GVGRPELVGTDPAYRNRGLVRTQFDVIHAWSRQRGHLLQGITGIPYFYRQFGYEMTVE
                     MPGRLFRRGKSPSLDLKPGETDPYTMRPAVEADLPFIAECEAAGTRAQPLTVLRDLDL
                     WRYELNGRSQFNIHRSEIRLITTAAGAPVGFLAHAPLIWGKAMLLSQLHLTPGVSWQA
                     VMPSVLRYQWAVARQYMDQNGGEVESVGVWMCRDHPAYPVVKNFLPETGIPYAWYLRV
                     PDLPAFLRHIAPALATRLAASPLAGHTGEMRLCFYRSGLYLKFEQGQLTQVQTEPVAW
                     HKADAAFPDQTFLHLLFGHRSVSDLRYMFGDFFVNSSHLPLLEALFPPRAAYLWPIS"
     gene            complement(1726..2052)
                     /locus_tag="PKL11_09840"
     CDS             complement(1726..2052)
                     /locus_tag="PKL11_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ13942.1"
                     /translation="MPVYTYRCANCDYQFDQTQRFSDDSLTVCPNCGQAMLHKVYSPV
                     GIVFKGSGFYATDHRSPSGAPRTAGSHSEEKSGESKAETSAPAAEPAKTPTPTPAPAP
                     SGSDAA"
     gene            complement(2213..3175)
                     /locus_tag="PKL11_09845"
     CDS             complement(2213..3175)
                     /locus_tag="PKL11_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ13943.1"
                     /translation="MDLNLETELDYDVVIIGGGPAGVTAAIYTARADLRTLVIDKGLT
                     AGALGITGKIANYPGIVEEISGAELLSRMRQQAASFGARFVQDKVQGVDLTSEPRQIY
                     ANNATYTARAVLIATGSMGRGQRVPGEDTLLGRGVSYCATCDAAFFRNQDVAVIGSSD
                     EAIEEALFLTRFARQVQLVSPTPELKAPPELIAELEQSPITVHLGAALRSIEGENRVS
                     GIKIAPRGQPEQILPVTGAFIYLQGGKPITDFLQGALPISEAGCLRVDAEYQTGLPGV
                     YAVGDVLCNHVKQAVVAAAEGAVAGMAIEKFLRGRKQLVVDWSK"
     gene            3304..3927
                     /gene="rdgB"
                     /locus_tag="PKL11_09850"
     CDS             3304..3927
                     /gene="rdgB"
                     /locus_tag="PKL11_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNZ13944.1"
                     /translation="MTDPTNVLLLLATTNPGKLREMVDILLDCAHTAPLPDRRLTIVS
                     PGELGKRLQVAETGQTYAENAALKAQAFCEATGLITLADDSGLEVEALGGAPGIYSAR
                     YAPQPGATDADRRALLLERLADFPRPWTARFRCAVCVAAPGLPLLQVEGDCPGEIIPT
                     ERGQNGFGYDPIFWLPELGCTMAELPDTQKNQISHRARAIRAAWGLF"
     gene            complement(3931..>4190)
                     /locus_tag="PKL11_09855"
     CDS             complement(3931..>4190)
                     /locus_tag="PKL11_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13945.1"
                     /translation="YFAILLISSGMFWRITMEGISIWSVLGIVFVIGVLLYITFTSKK
                     TKHDLPIAGGCAPVIDESKRPFAIHADEEPEGPPEVEEEKP"
BASE COUNT          828 a         1230 c         1365 g          767 t
ORIGIN      
        1 attaaacccc aaacgccctc gaggtgaggg cgttcttctg gcggggaggg cgggattcga
       61 acccgcggct gaggtaaatc ccagcactca cttagcaggc gagcccaatc ggccactctg
      121 gcacctcccc aatttgttaa aacgtccccc ctccagcgga gggagaggga ttcgaacccc
      181 cggtgggttg ccccacacct gttttcaaga caggcgccat aggccgctcg gccatccctc
      241 cagggcgcgg gaattgtatc ataactcccg cgatgcggca agacaggcac gcggccgcgt
      301 ttcagctaat gggccacaga tacgcggcgc gcggcgggaa gagggcttcc agcaggggga
      361 ggtgagacga attgacgaag aaatcgccaa acatgtaacg caggtctgat acgctgcggt
      421 gaccaaacag caggtgcaaa aaggtctggt cggggaaggc ggcatcggcc ttgtgccagg
      481 caaccggctc ggtttgaacc tgtgtgagct ggccctgttc aaatttgagg tacaggcccg
      541 agcggtagaa gcacagccgc atttcgccgg tgtgccccgc cagcggagaa gccgccaacc
      601 gcgtagccaa tgccggggca atgtgacgca aaaaagcggg caggtctggc acgcgcaggt
      661 accaggcata gggaatgcca gtttcgggca ggaagttctt gaccaccggg taagccggat
      721 ggtcgcggca catccacacc ccgaccgatt ccacctcgcc gccgttttga tccatgtact
      781 gccgggccac cgcccattga tagcgcagca ccgagggcat gaccgcctgc caggaaacgc
      841 cgggggtcag gtggagctgc gagagcagca tggctttgcc ccagatcagc ggggcatgcg
      901 ccaaaaagcc caccggggca ccggcggcag tggtgatcag gcggatttcg gagcggtgga
      961 tgttgaattg ggacctgccg ttcagctcat aacgccagag gtccaggtct cgcagaacgg
     1021 tcagcggctg ggcgcgtgtc ccggcggctt cacactcggc gataaagggc aggtcggcct
     1081 caacagccgg gcgcatggtg tacgggtcgg tctcaccggg cttcagatcc aggctgggag
     1141 atttgccgcg ccggaagagc cgcccgggca tttccacggt catttcgtag ccaaactggc
     1201 ggtagaagta ggggatgccc gtgatgccct gcagcaggtg gccacgctgg cggctccagg
     1261 catggatgac atcaaactgg gtgcgcacca ggccgcgatt gcggtatgcc gggtcggtgc
     1321 cgaccagttc ggggcggccc acgcccaggg acaggccgtc aaacgcccag gtctgggaga
     1381 tcagattcat ggcggagacg attttgccgg tcgattcttc gaccaccagg gtaaaatcat
     1441 ccggcgcatg ggtggggtgc tgaccattca acagatcgcg cgtccaggca gccagcgagt
     1501 cgtgcgcatc gggctcttcg tcgtcggcgg tgtgaatgcg ttgattaaat tcagccagtt
     1561 gatccgcgtc tgcggtgctg gagcggcgca ggatcaggcc atcgggcaga gggcgcggca
     1621 gggataattc ggcagggatg ggtgtggaca tgatggacct caaaatgggg ggcataaaaa
     1681 cagcctgggt aagactccca ggctgcggtg atgacggttg cggcgtcagg ctgcatcact
     1741 gcccgaaggg gcaggcgcag gggtgggggt cggagtcttg gctggctcgg cagccggggc
     1801 ggaggtttca gctttgctct cgcccgattt ttcttcgctg tggctgccag cggtgcgagg
     1861 cgcgccagag ggggagcggt gatcggtggc atagaagcct gagcccttga agacaattcc
     1921 aaccggcgaa tagactttgt gcagcatcgc ttgaccgcag tttgggcaaa ccgtcagcga
     1981 atcatctgag aagcgctggg tctgatcaaa ttgatagtca caattggcac aacgatacgt
     2041 ataaaccggc atacagtatt acctcaaaca tggttaaaac ccgcatcagg gccgcgccct
     2101 gaacgtccag tgattttacg actattttcg gcgctttgca agggcgcgcg gtcgccagcc
     2161 cggcaaatca ggacattcct catgcgcttt ggggttgaag gatcgcggcg ggttacttgc
     2221 tccaatccac caccaactgc ttgcggccgc gcaagaattt ctcgatggcc atgccagcca
     2281 cggcgccttc ggcggcagca accaccgcct gtttgacatg attgcacagc acatcgccca
     2341 cggcatagac gcccggcagt ccggtctgat attcggcatc cacgcgcagg cagcccgcct
     2401 cgctgatggg cagcgcaccc tgcaaaaagt cggtgatggg cttgccgccc tgcagataaa
     2461 tgaaggcccc ggtgacgggc agtatctgtt cgggctggcc gcgcggcgca attttgatgc
     2521 cgctgacgcg gttctcgcct tcaatgctgc gcagcgctgc gcccaggtgc acggtgatag
     2581 gcgactgttc cagttcggcg atcagttcgg gtggggcttt caattcgggc gtgggcgaga
     2641 cgagctgcac ctgccgggca aaccgggtga ggaacagcgc ttcttcaatc gcctcatcgc
     2701 tgctgccgat caccgccaca tcttgattgc ggaagaaggc ggcatcacag gtggcgcagt
     2761 acgagacgcc cctgcccagc agggtatctt cgccgggcac ccgctgaccg cggcccatcg
     2821 agccggtggc gatcagcacg gcgcgggcgg tgtaggtggc gttgttggca tagatctggc
     2881 gcggttcgct ggtcagatct acgccctgca ctttatcctg cacaaagcgg gcgccaaaac
     2941 tggcagcctg ctggcgcatg cggctgagca gttcggcgcc gctgatttct tcgacaatgc
     3001 cggggtagtt ggcgatcttg ccggtgatgc ccagggcacc cgccgtcagg cctttgtcaa
     3061 tgaccagggt gcgcaggtca gcgcgggcgg tgtagatggc tgcggtgacg ccagccggac
     3121 cgccgccgat aatcacaaca tcgtaatcca gttcagtttc caggttcagg tccatgaggt
     3181 gctcctcgat aaaatgacat gataattgca gtacgttgat ttggatgctg taaacggaga
     3241 aaagttatat ccaaccgccg ggcgcatctg ccggctgacc ggggctgtgg tagaatttca
     3301 gacatgaccg acccaaccaa cgtcctctta ctgctggcaa ccaccaaccc cggcaagctg
     3361 cgcgagatgg tggatattct gctcgactgc gcccacaccg cccccctgcc tgaccggcgg
     3421 ctgacgattg tcagcccggg cgaactgggc aagcgcctgc aggtggccga aaccgggcaa
     3481 acctacgccg agaacgccgc gctgaaggcg caggctttct gcgaggccac cggcttgatc
     3541 accctggcag acgattccgg gctggaggtg gaggcgctgg gcggcgcacc ggggatttac
     3601 tcagcgcgct atgcccccca gcctggcgcc accgatgccg accgccgcgc cctgctgctg
     3661 gagcggctgg cagatttccc ccgtccgtgg acggcgcgct tccgctgtgc ggtgtgcgtg
     3721 gcggcgccgg gcctgccgct gctgcaggtt gaaggcgact gccctggcga gattattcca
     3781 acggagcggg gccaaaacgg ctttggctac gacccaatct tctggctgcc cgagttgggg
     3841 tgcaccatgg cagagctgcc cgacacgcag aagaatcaga tcagccaccg ggcccgggct
     3901 atccgggcgg catggggatt gttttagcgg tcagggcttt tcttcctcaa cctcgggggg
     3961 gccttcgggc tcttcgtcag cgtggatggc gaaggggcgc ttgctttcat caatcaccgg
     4021 ggcgcagccg ccggcaatcg gcagatcgtg cttggtcttt ttagatgtaa aagtgatata
     4081 gagcagcacc ccaatcacaa agacgatgcc cagaacagac cagatgctga tgccttccat
     4141 ggtaatcctc caaaacatgc cggatgatat taacagtata gcaaaataat
//
LOCUS       DAOPVS010000165         4186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_21415_length_4186_cov_57.219802, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000165 DAOPVS010000000
VERSION     DAOPVS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21415_length_4186_cov_57.219802"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1918
                     /locus_tag="PKL11_09860"
     CDS             <1..>1918
                     /locus_tag="PKL11_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13946.1"
                     /translation="LLWLLSALTGWGARPDILWNEAAPPILAVQLALSLLIALVLGAL
                     LRRIEPVRRRRAEWFIALALWALAVFVWVREPMPRSYFAPGPYPPNDVYYPYSDAASY
                     DVSAQYVLLGQPLDSTAPNEKPVYGAFLTVLHLLAGQSYDLVTLIQVLVLALFPVILF
                     RLGRHFHLEAGLLVALLAIFKERNAIAATLSIRVSHSRLLLTEFPTALFVALICWLLV
                     EYLAAPDRRRLLPLAIGGWLGLGAMLRLNLLALVPMIIILVVLAFWRRPRAAWLFSLI
                     LIVSLAIPMIPWSAATSARWGQPAWVMKLKMVLDSRYAAPTVTPTANPQPQATPLPAT
                     EAQPAPASTPVPLATPVPQPAPLSQQSEICAGKTGLAEILCFAPAHWAHNNLMAVLTL
                     PHTLAWEDLPHTLQRDYWLDIRSWNGRLAADEAILMILNLALVALGIGFAWHRRRWAG
                     LAPLAFMQIYFVANALGRTSGGRYLTPVDWVVYFYAALGLCLIVDWVRTLLGFPAAPE
                     DSRPAAPAARRFPWLPAAALLLAFLIPALALPFMQRAFPAQLTPPSQSDLLDRLNASG
                     ALTQMNFSPQQVSDFAGSPEALLITGRGLYPRFYGINQGEPDSQATPQFAVRPYPRLL
                     FTLLTAQQPRVVELP"
     assembly_gap    1919..2018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2019..3421)
                     /locus_tag="PKL11_09865"
     CDS             complement(<2019..3421)
                     /locus_tag="PKL11_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNZ13947.1"
                     /translation="MSERLRQRLLDFGWATLALLLPITSLPLAARLMGGTMVAPPATL
                     ILAVLMLAWLLPRWIKGGTLPRQSLPLLWFALAALLSWAVSYFIYLPPFRNINRLTNE
                     LSAVLSLAIGICFYLAAASLPAADGRLQKLLRWINWGGVILIAWSLVQAAAFMVNAEV
                     YPDWMQRIQEAISASGILYKKRVTGLAYEPSWLANQLNMLYLPLWLAATLRRTSAHTF
                     RILRLPLETWLLAGGVLVLGLSFSRIGLASFLLVVAWLLLRANLWLVRWLLKRIARRW
                     PDAAERQALRRALYTGLILALLLIYLGLAGGAAYGMSRLDPRMADLFSLLADKPNFNE
                     LAHGLIFGERAIFWQVGLDVFGSYPVLGVGLDHAGYFIPERLPPFGWTMAEPHKLYYQ
                     TALPNLMSLWIRLLAETGIIGFALFVSWLVVLGASAAALERSADRRLQVLGLAGQFAL
                     IALLGEGLSIDSFALPYL"
     gene            complement(3414..>4186)
                     /locus_tag="PKL11_09870"
     CDS             complement(3414..>4186)
                     /locus_tag="PKL11_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13948.1"
                     /translation="DFSPRQTLERTLRGWPIIAALMLAGVLLGWGFSLLSAPIYEAQA
                     EILTGINSAQVGSVDDEELDLIINTAGDVMDSTLVRQAVLDAAAARGWPLELTAFSGP
                     LTLERKAQSWVFRVQDKDPQRAADLANLWADEALAALDEAAQYARLAEAEQRALDSLV
                     TCWQYNVSQPQGSAACAPLTAAELQDQIVELNTAVQDARAEAQGLFPGINYALNQEAS
                     RPTHPLHYARTNLMLAGAGLGLLIGLGLLFQPWRPRRV"
BASE COUNT          750 a         1358 c         1152 g          826 t
ORIGIN      
        1 ccttgctctg gctcctgtct gcgctgaccg gctggggcgc ccgccccgac atcctgtgga
       61 atgaagccgc cccgcccatc ctggcagtgc agttggcgct cagtttgctg attgcgctgg
      121 tgttaggggc cttgctccgc cggatagagc cggtccgccg ccggcgcgct gaatggttca
      181 ttgccctggc gctctgggcg ctggcagtct ttgtctgggt tcgcgaaccc atgccgcgca
      241 gctattttgc acccggtcct tacccaccca acgatgtgta ttatccctat tccgatgcgg
      301 catcttatga tgtctcggcg caatatgtcc tgctcggaca gccgctggat tctaccgcac
      361 ccaatgaaaa acccgtctat ggtgcctttc tgacggtttt acatctgctg gcagggcagt
      421 cttatgatct ggtgaccctc attcaggtcc tggttctggc gctcttcccg gtgattttgt
      481 tccggctggg gcgtcacttt caccttgagg ctggattatt ggttgccttg ctggcgatct
      541 tcaaagagcg caacgccatt gctgccacgc tgtccattcg ggtttcgcat agccgattat
      601 tgctgaccga attcccgacg gctctttttg ttgccctcat ttgctggctc ctggtggaat
      661 accttgccgc cccagaccgc cgccgcctgc tccccctggc cattggcggc tggctgggcc
      721 tcggcgccat gctgcggctc aacctgctgg ccctggtgcc gatgattatc atcttggtgg
      781 tgctggcttt ctggcgccgc ccgcgggcgg cctggctttt cagcctgatt ctgatcgtgt
      841 cacttgccat cccgatgatc ccctggtctg ccgccacttc ggcgcgctgg gggcagcccg
      901 cctgggtgat gaagctgaag atggtgttgg attcgcgcta cgccgcgcca acggtaaccc
      961 ccaccgccaa cccacagcca caggcaacgc ccctgcccgc gactgaagct cagccggcgc
     1021 ctgcatccac gcctgttccc ttggcaacgc ctgttccaca accggcgccg ttatcgcagc
     1081 agagcgaaat ttgcgccgga aaaactggcc tggcagaaat tctttgcttt gccccggcgc
     1141 attgggcgca taacaacctg atggccgttc tgactctgcc gcataccctc gcctgggaag
     1201 atctgccgca caccctgcag cgggattact ggctggatat ccgctcctgg aatggacgcc
     1261 ttgccgctga cgaggctatt ttgatgatcc tcaatttggc gctggttgcc ctgggcatcg
     1321 gctttgcctg gcaccgccgc cgttgggctg gcctcgcgcc gctggccttt atgcagattt
     1381 atttcgttgc caatgctctg gggcgtactt ccggcgggcg ttatctgacc cccgtggact
     1441 gggtggttta tttctatgct gccctgggct tgtgcttgat tgtggattgg gtgcggacgc
     1501 tgctgggctt cccggctgca cccgaagatt cccgcccagc cgcaccggca gcccggcgtt
     1561 tcccctggct tccagcggct gcgctgctcc tggcttttct gatcccggct ttggcgctgc
     1621 ctttcatgca gcgcgcattt ccggcgcagc ttacaccacc atcccaatca gatttgctgg
     1681 atcgcctgaa cgcctctggg gctcttacac aaatgaactt ctcgccccaa caggtgagtg
     1741 atttcgctgg ctcccccgag gccctactga taaccggtcg cggcctctat ccccgctttt
     1801 atggcatcaa ccagggcgaa ccggattctc aggccacgcc ccagttcgcc gtgcgcccat
     1861 acccgcgtct gctcttcacc ctgttgactg cgcagcagcc ccgtgtggtt gagcttccnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gtagggcagg gcaaacgagt
     2041 caatgctcaa accctcgcca agcagcgcaa tcaacgcaaa ctgtccggcc aatcccagta
     2101 cctgcagccg gcggtctgcg ctgcgttcca gcgcggccgc cgaagcgcct aaaaccacca
     2161 gccagctcac aaacagtgca aaaccgatga tccccgtttc tgccagcagc cggatccaca
     2221 ggctcatcag gtttggcagc gcggtttgat agtacagctt gtgcggttcg gccatggtcc
     2281 acccaaaggg cggcagcctt tcagggatga aataccctgc atggtccaac cccaccccca
     2341 acaccgggta actgccgaac acatccagtc ccacttgcca gaagatggcc cgctcgccaa
     2401 aaatcaaccc gtgcgccagc tcattgaagt tgggcttatc tgccagcagt gaaaatagat
     2461 ctgccatgcg cgggtccagg cggctcatgc cataggctgc gccccccgcc agccccaggt
     2521 agatgagtaa cagcgccagt atcaaacccg tgtacagtgc cctccgcagg gcctggcgtt
     2581 ccgccgcgtc tggccagcgc cgggcaatcc gtttgagcag ccagcgcacc agccacaggt
     2641 tggcgcgcag taacagccag gcaaccacca gcaggaagct tgccagtcca atccgtgaga
     2701 atgacaaacc cagcaccaac accccccccg ccagcagcca ggtttccagc ggcagccgta
     2761 aaatccgaaa cgtgtgtgct gaggtacgcc gcaaagtggc tgccagccac aaaggcagat
     2821 acagcatatt caactggttt gccagccagg atggttcata agccagcccg gtgacgcgct
     2881 ttttgtagag aatgccgctg gccgagatgg cttcctgaat ccgctgcatc cagtcggggt
     2941 aaacttcagc attcaccatg aaggccgccg cctgaaccag cgaccacgca atcaggatga
     3001 ccccgcccca gttgatccag cgcagcagct tctgcaggcg gccgtccgct gctggcaggc
     3061 tggctgctgc caggtaaaag cagatcccaa ttgccagcga cagcacggcg gataattcat
     3121 tggtcagccg gttgatattc cgaaacgggg gcaaataaat gaaataagac acagcccacg
     3181 aaagcagagc tgccagagca aaccacagca gcggcaggct ctggcgcggc agtgtgccgc
     3241 ctttgatcca gcgtggcagc agccaggcga gcatcaacac agccagaatc aacgtggcgg
     3301 gcggcgccac catcgtgccg cccatcagac gggccgccag cggcaggctg gtgatgggta
     3361 gcagcagcgc cagggtcgcc cagccaaaat ccagcaggcg ctggcgcagg cgttcagaca
     3421 cggcgcggcc tccagggttg gaacaataac cccagcccga tcagcagccc cagcccggct
     3481 cccgccagca tcaaattggt gcgggcataa tgcagcgggt gggtgggccg gctggcttct
     3541 tgattcaacg cataattgat ccctggaaac aacccctggg cctcggcgcg tgcgtcttgg
     3601 accgcggtgt tcaactccac aatctgatct tgcagctcag cagcggtcaa tggcgcacag
     3661 gctgccgatc cctgtggctg cgatacattg tattgccagc aggtgaccag tgagtccagg
     3721 gcgcgctgct ccgcttccgc taaccgggca tattgggcag cctcatccag cgcagccagc
     3781 gcttcatcag cccacagatt agccaggtct gcggcgcgct gcggatcttt atcctgcacc
     3841 cggaagaccc aggattgggc tttgcgctcc agcgtcagcg ggccagaaaa agccgtcagc
     3901 tccaggggcc agccgcgcgc cgccgcggca tccaaaacgg cctgccgtac cagggttgaa
     3961 tccatcacgt ccccggcggt gttgatgatc aaatccagct cttcatcgtc cacgctgccc
     4021 acctgtgccg aattgatccc ggtcaggatc tcggcctgcg cttcataaat gggggcgctc
     4081 agcaggctga acccccagcc caacagcacc cccgccagca tcagcgcggc aatgatgggc
     4141 caaccgcgca atgttcgttc aagtgtctgt cgtggtgaaa aatcag
//
LOCUS       DAOPVS010000166         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_21769_length_4132_cov_61.964189, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000166 DAOPVS010000000
VERSION     DAOPVS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21769_length_4132_cov_61.964189"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKL11_09875"
     CDS             <1..809
                     /locus_tag="PKL11_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ13949.1"
                     /translation="DLVSHYPIHVIPLTLNWDGRTYRDGVDIGATEFYARLSQSETLP
                     TTSQISAGEFEKTINKLLAAGNDVLLLPISSGISSTYETAASVLEHFPRERVALLDTK
                     LVSMALGFQVLAAARAAAAGADLSECLAAATEAYSQIGVYFTIDTLKYLAAGGRINSA
                     KRLVGTALNLKPILEIREGKIELVTSVMTRKKSIATLLQLVEAGIAGRTPVRISVFHA
                     LAIDAAEELLETARKRFKPVECILSEVSPVVGSHTGPGTLSIAYQAGGKI"
     gene            complement(847..1584)
                     /locus_tag="PKL11_09880"
     CDS             complement(847..1584)
                     /locus_tag="PKL11_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13950.1"
                     /translation="METRTLILFLSELVGAIAVTLLLTLSPRFKRGRAVQFARPRQEG
                     LFGLGVAVLLGLAAYAFLRGPGASLTYDETTLAGLGVRLGIGAAALLVVALILRLRKH
                     PLSSVRWSRPMLMPGVQLGLAVGFLTLFLRNILPELFKGITMDQGYALLVWLGIALAE
                     ESLFRGYVQLRLEAWLGERWGWLAASGLSTLWLLLFIGGQPLSALLPRLGLLLLQALV
                     LGWMAQRSRGVLGGAIYRAMSGWLMMF"
     gene            complement(1588..2031)
                     /locus_tag="PKL11_09885"
     CDS             complement(1588..2031)
                     /locus_tag="PKL11_09885"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNZ13951.1"
                     /translation="MFRKTTPQTPTAAAPAERVTSVLGPGILWQGNLRGSGGIRIEGT
                     FDGEIAIRGLVVVGETGRVNCENLRANTVIVAGAVRGNVTAERIEIRATGRIWGDVTT
                     VAFSTEEGAFLRGQVLMEERVELNLEEPDAETPPAVEPTEPAPGA"
     gene            complement(2047..3666)
                     /locus_tag="PKL11_09890"
     CDS             complement(2047..3666)
                     /locus_tag="PKL11_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNZ13952.1"
                     /translation="MSKRYPLIAAAMFIAIFLLALAPLPASSSPTALTATPNPGLPFG
                     SPANVVHGLYFYSVDCSHCMAVLTDLIQPLQAQYGSTLDLRLLEIATPANYELMALAE
                     DTFQVSAAERDIPVLVLGDQIMIGETAIRSQAPDQIARAAALGGLDWPAIPGLDPAQL
                     VSTVPSGVTTATACPPTGGDGCAAPLPIYAAYFYQTGCKECSMVDVDLQYLREKYPQL
                     IIEEFNIFDDAALGEWLAERAGRDDFHSPALFIGDQAFIDQQEINPAVLEPVLDALAP
                     GGSTRTWADFDPAAAKNNLLARFRSMGWLAVAFAGLVDGLNPCAFATLVFFVSYLTLS
                     GRKGKAVLAVGLAFTLGVFLAYLVIGLGFYKVLDLMGGWLTTLSRWVYALTALLCLGL
                     AVLSFLDFLKARRGQIEDMALNLPKPLRMRINATIREGRKTRAYVAGAFVTGILISFL
                     ELACTGQIYLPTIIFVSSLPELRLQAVGYLVLYNLLFIVPLAVVFVLAYYGTTSKDLT
                     AFLQQRAASVKLGMMILFITLAAWLGASLLG"
     gene            complement(3809..>4132)
                     /locus_tag="PKL11_09895"
     CDS             complement(3809..>4132)
                     /locus_tag="PKL11_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNZ13953.1"
                     /translation="FASLLIPAAYAKVATEGTIKAGLQFKEVIATVKASIGSYLLALL
                     GTLLATAIIAPLGSIACGLGVLLTVPFAMTVNAALYGMAYNVAQGNRGLSAPLPPVDT
                     NLNTL"
BASE COUNT          850 a         1343 c         1258 g          681 t
ORIGIN      
        1 ccgatctggt ctcacattat cccatccacg tcatcccgct gaccctgaac tgggacggcc
       61 ggacctaccg cgacggcgtg gatattggcg ccaccgagtt ctacgcccgc ctgtcacaat
      121 ctgaaaccct gcccaccacc agccagattt cagccggtga gtttgagaaa accatcaaca
      181 agctgctcgc cgccggtaat gacgtgctgc tgctgcccat ctccagcggc atctccagca
      241 cctacgagac tgcagccagc gtgctggaac acttcccccg cgagcgggta gccctgctgg
      301 ataccaaact ggtctcgatg gcgttgggct ttcaggtgct ggctgccgcc cgcgccgccg
      361 cggcaggcgc cgacctgagc gaatgtctgg cggcggcaac cgaggcctac agccagattg
      421 gggtctattt caccatcgac acgctcaaat acctggccgc gggcgggcgc atcaacagcg
      481 ccaagcggct ggtgggcact gcgttgaatc tcaagcccat cctggagatc cgggagggca
      541 aaattgaact ggtgaccagc gtgatgacgc gcaagaagtc tattgcaacg ctgctgcaac
      601 tggttgaagc cggcatcgcc ggacgcaccc cggtgcgcat ctccgtcttt cacgccctgg
      661 ccattgacgc cgccgaagaa ctgctcgaaa ccgcccgcaa gcgcttcaag cccgtcgagt
      721 gcatcctctc cgaggtcagc ccggtggtgg gttcgcacac gggccccggt acactgtcta
      781 tcgcctacca ggcaggtggg aaaatataaa ttccggggtt ccacgggcac gtggaatcac
      841 ccttaatcaa aacatcatca gccagccgct catggcgcgg tagattgcac cgcccagcac
      901 cccacggctg cgctgcgcca tccaacccag caccagcgcc tgcagcagca gcagccccag
      961 gcggggcagc agcgcgctca gcggctgccc gccgatgaac agcagcagcc acagcgtaga
     1021 gaggccgctg gcagccagcc agccccagcg ctcccccagc caggcttcca gccggagctg
     1081 cacatagcct cgaaaaaggc tctcttctgc cagcgcaatc cccagccaga ccagcagggc
     1141 atagccctga tccattgtga tgcccttaaa cagctcgggc aggatattcc gcaaaaacaa
     1201 cgtcagaaaa cccactgcca ggcccaactg cacccccggc atcagcatgg ggcggctcca
     1261 gcggacgctg gacagcggat gtttgcgcag ccgcaggatg agcgccacca ccagcagtgc
     1321 cgcggcgcca atgcccagcc gcacacccag acctgccagg gtcgtctcgt catacgtcag
     1381 gctggcgccc gggccgcgca gaaaggcata agccgccagg cccagcagca ccgccacgcc
     1441 cagcccaaac agaccttctt gccgggggcg ggcaaactgc accgcccgcc cgcgcttgaa
     1501 ccgcgggctg agagtcagca gcagcgtgac ggcaatggcg ccgaccagtt cactcaaaaa
     1561 caggatcagg gtgcgggttt ccatgggcta agctcccggc gccggttcag tcggctcaac
     1621 ggctggcggc gtttcggcgt ctggctcttc caggttcagt tccacgcgtt cttccatcaa
     1681 cacctggccg cgcaggaaag ccccctcttc cgtgctgaag gccacggtgg tcacatcgcc
     1741 ccagatgcgc ccggtggcgc gaatttcaat ccgctcggcg gtcacattgc cgcgcacggc
     1801 cccggccaca atcaccgtgt tggcgcgcag gttttcgcag ttcacccggc ctgtctcgcc
     1861 gaccaccacc agcccgcgaa tggctatctc gccgtcaaaa gtcccttcaa tgcgaatgcc
     1921 gccgctgccg cgcaggttgc cctgccacag aatgcccggc cccagcaccg aggtgaccct
     1981 ttcggcaggg gcggcggcag tgggcgtttg gggagtggtt ttacgaaaca tggggggtcc
     2041 tttctattat cccagcagcg atgcgcccag ccaggcagcc agggtgatga acaggatcat
     2101 catacccagt ttgaccgaag cagcgcgctg ctgcaggaaa gccgtcaggt ctttggaggt
     2161 ggtgccgtaa tacgccagca caaaaaccac cgccagcggc acaataaaca gtagattata
     2221 caataccaga tagcccaccg cctgcaggcg cagttcgggc aggctgctga cgaagatgat
     2281 ggtgggcagg taaatttggc ccgtacaggc cagctccagg aaggagatga ggatgcctgt
     2341 cacaaatgcc ccggccacat aggcgcgggt tttgcggcct tcacggatgg tggcgttgat
     2401 gcgcatgcgt aagggtttgg gcaggttgag cgccatgtct tcaatctgac ctcggcgcgc
     2461 tttgagaaaa tcgaggaagc tgagcactgc caaccccagg cacagcagcg ccgtcagcgc
     2521 atagacccag cggctgaggg tcgtcagcca gccgcccatc aggtccagca ccttgtagaa
     2581 gcccaggcca atcaccaggt aggccagaaa gacccccagc gtgaaggcca gccccaccgc
     2641 cagcacggcc ttgcctttac gcccgctcag cgtgaggtac gagacgaaaa agaccagcgt
     2701 ggcaaaggcg caggggttca gcccgtccac cagcccggca aaggccactg ccagccagcc
     2761 cattgagcgg aagcgggcca gcaggttgtt tttggcggcg gcggggtcaa aatcggccca
     2821 ggtgcgtgtt gagccgccgg gcgccagggc gtccagcacg ggctccagca cggcggggtt
     2881 gatttcctgc tgatcgatga aagcctggtc tccaatgaac agcgccgggg agtgaaaatc
     2941 atcccgcccg gcccgttctg ccagccattc gcccagtgcg gcgtcatcaa agatgttgaa
     3001 ttcttcgata atcaactgcg gatatttctc acgcaggtat tgcaggtcca catccaccat
     3061 gctgcattct ttgcagccgg tctgataaaa ataagccgcg tagatcggca gcggggcggc
     3121 acagccatca ccgccggtgg gggggcaggc cgttgccgtg gtcaccccgc ttggcaccgt
     3181 actgaccagt tgggcagggt ccagtccggg gatggcgggc cagtccagcc cgcccagggc
     3241 ggcggcgcgg gcaatctggt cgggggcctg gctgcggatg gccgtctcgc cgatcatgat
     3301 ctggtcgccc agcaccagca cggggatatc gcgctcggcg gcagagacct ggaaggtatc
     3361 ttctgccagg gccatcagct cgtaattggc aggggtggca atctccagca ggcgcagatc
     3421 cagggtgctg ccgtattgcg cctgcagcgg ctgaatcagg tcggtcagca cggccatgca
     3481 gtgcgagcag tccaccgaat agaaataaag gccgtgcacc acgtttgccg ggctgccaaa
     3541 aggcagcccc gggttgggcg tggcggtgag ggccgttggc gaactgctgg cgggcagcgg
     3601 cgccagggcc agcagaaaaa tggcgatgaa cattgcagcc gcaatcagcg gatagcgttt
     3661 tgacacaccg atctccatga gaccaaaaat ctggaggaat tatactatca aatttttcct
     3721 gagcgcccat ccaccctcag accatctgta atcaaacagc ggcaagccag gcctgccgct
     3781 gttagcattt gattgatcct gctctgattt agagcgtgtt gagattggta tccacgggcg
     3841 gcagcggggc cgagaggccg cggttgccct gcgccacatt gtaggccatg ccatacaacg
     3901 ccgcgttgac ggtcatggca aacggcaccg tcagcagcac acccaggccg caggcaatgc
     3961 tgcccagcgg ggcaatgatg gcggtggcca gcagggtgcc cagcagcgcc agcaggtacg
     4021 acccgatgga cgccttgacg gtggcaatga cctctttgaa ctgcaagcca gccttgatgg
     4081 tgccttcggt ggctactttg gcgtaggccg ccgggatcag cagcgaggca aa
//
LOCUS       DAOPVS010000167         4114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_21887_length_4114_cov_59.353043, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000167 DAOPVS010000000
VERSION     DAOPVS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21887_length_4114_cov_59.353043"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKL11_09900"
     CDS             <1..509
                     /locus_tag="PKL11_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13954.1"
                     /translation="GWWFVIANLFNSAWIFAWHYLQFGLSVVLMLGLLVSLLVIYERI
                     GRRAASRVETWTVNVPFSIYLGWITVATVANIASLLISLGWNGFGIPVVLTVAMIGIA
                     TLLGVLMILLKREIAYPLVLIWAFAGIAAVRADVPLVEIGAIVAAALLLLVMLARIGI
                     GLKQRAKA"
     gene            complement(577..1269)
                     /locus_tag="PKL11_09905"
     CDS             complement(577..1269)
                     /locus_tag="PKL11_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ13955.1"
                     /translation="MQTMNVSVIIPVYNEFGTIRDVIAAVQATGIPTEILAVDDGSTD
                     GTRALLAQMDGQGGVRVILHAQNQGKGAAVRTGIQNARGDVLIIQDADLEYDPQEYTH
                     LLQPIAAGQTDVVYGSRFLGSQRRQFLFWSMLGNRLLTFATNVLYFTRLTDMETCYKV
                     FRRSVVQGITLHARRFDFEPEFTAKLLKRRIRILEIPIAYNPRDYSAGKKIHLKDAFQ
                     ALWTLIKYRFVE"
     gene            1425..2747
                     /locus_tag="PKL11_09910"
     CDS             1425..2747
                     /locus_tag="PKL11_09910"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HNZ13956.1"
                     /translation="MKPLKLPGPVARSIIDRDKKVISPSYPRGYPFVMDHGRGVEVWD
                     VDGNRFLDFAAGIAVVSTGHSHPKVVKAIQEQAEKFIHISSDFYHSKWVELGEALDKY
                     APFKEDAVSFMTNSGTESVEAAIKLARYHTRRSSFIGFLGGFHGRTMGAVTFTASKPH
                     YRRGFLPLMNGVYHAPFPDNYHPILVGRPGEDYGETVVRYIEDEILTRLIPADEVAGI
                     LVEPIQGEGGYVVPPAGFFPALRKLCDKHGILLIADEVQAGMGRTGKWWSIENFGVEP
                     DIITSAKGIASGMPLGAMVARKKVVTWPKGAHGNTYGGNPISCAAALATMDLIENGYR
                     QNAAEVGAYALELLKNLQAKHPSIGEVRGIGLMLGVEFVKDQQTHEPDGKLRDLVVDL
                     AYEYGLLTLGCGNGVIRISPPLCVTRGEMDEAMAIFDHAIGLAEEKLR"
     gene            2748..>4114
                     /locus_tag="PKL11_09915"
     CDS             2748..>4114
                     /locus_tag="PKL11_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNZ13957.1"
                     /translation="MGLQMLPDFRVRQRDMLLEIARALAEELDLDALLGRVLKYSVDL
                     LGGQAGLIALRESEGWRVAAHQGLPSPYLQYLIPLLDQIPENDDPAKVELPEISRVVQ
                     ELTRMASLGTLSGVGLPLVARQRVVGVIFIFRSYLNMFSANDQALLRSFANQAALAVQ
                     NAQLFAEVTHEKQRLDTLLNSAADGILALTADQQVERVNPALAHMLGKDASGLIRQPH
                     DQVIRWERPPEGLTLEQAMAGGWPLTPNATLYVEGDLARTPDQPPLPVGITYAPVMSA
                     EGTLLQILGTVRDITRFRQAEELKSTFISIISHELKTPVALIKGYTSTLRREDATWDR
                     EIVQESLQVIEEESDRLSVLIENLLDASRLLAGGMALKRSDVSLPVLAERLTRRLQTQ
                     TQAHRLVVSFPEDFPVILADEQRMEQVLSNLISNAIKYAPGGEIRIQGQVRPDIVIVC
                     VSDEGP"
BASE COUNT          818 a         1097 c         1328 g          871 t
ORIGIN      
        1 tgggctggtg gtttgtgatt gccaatctat tcaacagcgc ctggattttt gcctggcatt
       61 atctgcagtt tgggctgtcg gtggtgctga tgctggggct gctggtctcg ctgctggtga
      121 tttatgagcg cattggacgg cgggcagcca gccgggtgga aacctggacg gtgaacgtgc
      181 ccttcagcat ctacctgggg tggattaccg tggcaacggt tgcaaatatc gcctcgctgc
      241 tgatctctct ggggtggaac ggatttggca tcccggtggt tttgacggtg gcgatgattg
      301 gcatagcaac cctgctgggt gtgctgatga tcctgttaaa gcgcgagatt gcctacccgc
      361 tggtgctgat ctgggcgttt gcggggattg ctgccgtacg ggcggatgtg ccgctggtgg
      421 agataggcgc cattgtggcc gcagctttgc tgctgctggt gatgctggcg cggattggaa
      481 ttggcctcaa gcagcgcgcg aaggcatagt tatgggagta aaaaacgggc accccggcgg
      541 gtgcccgttt tagttttaag ccattgtccc aagttcttac tcaacaaaac ggtatttgat
      601 caaggtccag agggcctgga aggcgtcttt gaggtggatt tttttaccgg ctgaataatc
      661 ccgcgggttg taggcgatgg ggatttccag aatgcggatg cggcgcttga gcagcttggc
      721 ggtgaactcg ggttcaaagt caaaccggcg ggcatgcagg gtgatgccct ggaccacact
      781 gcgccggaag accttgtagc aggtttccat atcggtgagg cgagtgaagt agagcacgtt
      841 ggtggcaaag gtgagcagac ggttgcccag catgctccag aaaaggaact ggcggcgctg
      901 gctgcccagg aagcgcgaac catagaccac atctgtctgc ccggctgcga tgggctgcag
      961 caggtgagtg tattcctgcg ggtcatattc caggtcggca tcctggataa tcaagacatc
     1021 gccgcgggcg ttctggatgc cggtgcgaac cgcggcgcct ttgccctgat tttgggcgtg
     1081 cagaatgacg cgcacgccgc cctgcccatc catctgagcc agcagtgccc gggtgccatc
     1141 ggtggagccg tcatccactg ccaggatttc cgttggaata cccgtcgcct gcacggcagc
     1201 aatcacatct cgaattgtgc caaattcgtt ataaaccggg ataatcaccg acacgttcat
     1261 tgtttgcatt atatcaggga atatcggcgg caatgggtga gtaaaataca gtaaagatgg
     1321 gatggaaggc aattatccgg gctgcgggcg gatgatataa tcggtacacg gcgcaatcca
     1381 tggcgaattc cgcccatttt ccaaaattct tgcaggagga tcatatgaag ccccttaaac
     1441 tgccagggcc agttgcccga agcattattg atcgagataa gaaggtcatc tcgccctcat
     1501 acccgcgcgg gtatccgttt gtgatggatc acggacgggg tgttgaggtg tgggacgtag
     1561 atggcaaccg gtttctcgat tttgctgcgg gcattgcggt ggtcagcacg ggccacagcc
     1621 accccaaggt ggtgaaggcc attcaagaac aggctgaaaa gttcattcac atttcgtctg
     1681 atttttatca cagtaaatgg gttgagctgg gtgaagcgct ggacaaatac gcacctttca
     1741 aggaagatgc ggtcagcttc atgaccaact cgggcaccga gagcgtggaa gccgccatca
     1801 agctggcgcg ttaccacacc cggcgctcgt ctttcattgg ctttttgggc ggtttccacg
     1861 ggcgcaccat gggtgcggtt acatttacag ccagcaaacc gcactaccgc cgcggctttt
     1921 taccgttgat gaacggcgtc tatcatgcgc ccttcccgga taattatcat cccatcctgg
     1981 tgggccgccc cggagaagat tacggcgaga ccgttgtacg ttacattgaa gatgaaattc
     2041 tcacccggtt aattccggct gatgaagttg cgggcatcct ggttgaacca atccaaggcg
     2101 agggtggtta tgttgtgccg ccggcgggct tcttcccggc gctgcgcaag ctctgcgaca
     2161 agcacggcat tctgctgatt gccgacgaag tgcaggcggg gatgggccgc actggcaagt
     2221 ggtggtccat tgagaacttt ggagtcgaac ccgacattat cacttcggcg aagggcattg
     2281 cctcgggcat gccgctgggc gccatggtgg cacgcaagaa ggtggtcacc tggcccaagg
     2341 gtgcgcatgg caatacttac ggcggcaacc cgatctcctg cgcggctgcg ctggcgacca
     2401 tggacctgat tgaaaacggt taccggcaga atgctgccga agtgggcgcc tatgcactgg
     2461 aactgttgaa aaatctacag gccaagcacc cctcgattgg cgaagtgcgc gggattggcc
     2521 tgatgctggg cgttgaattt gtcaaagacc agcaaacgca cgagccggat ggcaaactgc
     2581 gcgacctggt ggttgacctg gcctatgaat acgggctgct gacactgggc tgcggtaacg
     2641 gcgtcatccg catttcgccg ccgctgtgcg tgacccgcgg cgagatggac gaggcgatgg
     2701 caatcttcga tcatgccatt ggcctggccg aggagaaact gcgctaattg gggctgcaaa
     2761 tgctgcctga ttttcgcgtc cgccagcggg acatgctgct tgagattgcc cgcgccctgg
     2821 ccgaggaact cgacctggac gccctgctgg gacgcgtctt gaaatattcg gtggatctgc
     2881 tcggcggaca ggccgggctg atcgccctgc gggagagcga gggctggcgc gtggctgcac
     2941 accagggcct gccctcgccc tacctgcagt atctgatccc gctgctggat cagattccag
     3001 agaacgacga cccggccaag gtggaactgc cagagatcag ccgggtggtg caggaactga
     3061 cgcgcatggc cagcctggga accctgagcg gggtggggct gccgctggtg gcgcgccagc
     3121 gggtggtggg ggtgatcttt atcttccgca gttacctcaa catgttttcc gccaacgatc
     3181 aggcgctgct gcgttcattt gccaatcagg ctgcgctggc cgtgcaaaac gcccagcttt
     3241 tcgccgaggt gacgcacgaa aaacagcgtc tggacaccct gctgaactcg gctgctgatg
     3301 ggattttggc cttgaccgct gaccagcagg tggagcgggt taacccggcg ctggcgcata
     3361 tgctggggaa ggatgcttcc gggttgatcc ggcagccaca tgaccaggtc atccgctggg
     3421 agcgcccgcc tgaaggtcta acgctggagc aagcaatggc ggggggctgg ccgctgacgc
     3481 ccaatgccac gctgtatgtg gagggcgacc tggcccgcac acccgaccag ccgccgctgc
     3541 cggtgggcat cacctatgcg ccggtgatgt ctgcagaggg cacgctgctg caaattctgg
     3601 gcaccgtgcg cgatatcacc cgcttccggc aggccgaaga actgaaaagc acattcatct
     3661 caatcatcag ccatgaactc aagacaccgg tggcgctgat caaggggtac accagcaccc
     3721 tgcggcgcga ggatgccacc tgggatcggg aaattgtgca ggagagcctg caggtcattg
     3781 aggaagaatc ggaccggctg agtgtgttga ttgaaaacct gctggacgcc tcacggctgc
     3841 tggcaggggg gatggccttg aagcgctcgg atgtttccct gcctgtgctg gcagagcgcc
     3901 tgacccggcg tttacaaaca cagacacagg cgcacaggct ggtggtcagc ttcccggagg
     3961 atttcccggt gatcctggca gacgagcagc gcatggaaca ggtgctgtct aacctgattt
     4021 ccaatgccat caaatacgcc cccggggggg agatccgcat tcaggggcag gtgcgcccgg
     4081 atattgtgat tgtgtgcgtg agcgatgagg ggcc
//
LOCUS       DAOPVS010000168         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_23169_length_3947_cov_60.362539, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000168 DAOPVS010000000
VERSION     DAOPVS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23169_length_3947_cov_60.362539"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..106)
                     /locus_tag="PKL11_09920"
     tRNA            complement(25..106)
                     /locus_tag="PKL11_09920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Leu,seq:caa)
     gene            174..1244
                     /gene="ychF"
                     /locus_tag="PKL11_09925"
     CDS             174..1244
                     /gene="ychF"
                     /locus_tag="PKL11_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNZ13958.1"
                     /translation="MRLGIIGLPQSGKTTLFNALTRGTQPTSPASGRIEVHTAVVDVP
                     DPRVDRLSAMFKPKKTIYAKVTYADIAGLDGSAGKSGISGVLLNQLTQMDGLIHVVRC
                     FEDDMVPHPQDTIDPIRDIATMDSEFILNDLIAVERKLERLTEERKKGAGRDKSIIER
                     ETTLFERFQAALSAETPLRDVTITAEEEKMLSGFGFLSRKPILIVLNLSEGQRPPEIS
                     YAHQSSQIVALQARLEADIAQLPPDEAQVFLAEYGIEEPSLNRMIRLSYDLLGLQSFF
                     TVGPDEVRAWTVQRGATAPEAAGVIHTDLQKGFIRAEVAAYDDLITLGGLNEAKTHGR
                     LRLEGKEYIVKDGDILNIRFNL"
     gene            1254..2396
                     /locus_tag="PKL11_09930"
     CDS             1254..2396
                     /locus_tag="PKL11_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNZ13959.1"
                     /translation="MDTAALSKPIWIDRRQALERLAAELLRHTQVAVDTESNSLFAYQ
                     EQVCLIQFSTPTADYLLDPLALPDLSPLAPLFANPAIEKIFHAAEYDLICLKRDFDFT
                     FANIFDTMQAARILGKTAFGLGAILEESFGITLNKRYQRANWGERPLSAPMLDYARMD
                     THYLIPLRARLAAELETSGRKPMAQEDFTRLCAVEAGSLHENGQDWWRVSGGRDLTPQ
                     QAAVLQALCDYRDQQARNANLPRFKVLNDDVLITLAETCPQTLAELKPIPHLGPRNIE
                     KHGVRLLAAIQRGLKSPPLHRPSHPRPDDAYLFRLDLLREWRKVTGRELGVDSDVILP
                     RDTMEHIAQANPATLPELQDQMPLLPWRFNRFGAEILRVLPPKVKS"
     gene            2393..3787
                     /locus_tag="PKL11_09935"
     CDS             2393..3787
                     /locus_tag="PKL11_09935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ13960.1"
                     /translation="MKLTIYGAGQTVTGSQYVLEVNGQTLLLECGLYQGHRSDTFAKN
                     LNFEFSPRTVDAAILSHAHIDHSGNLPNLVKQGYSGPIYVTPATAGLCELMLMDSAHI
                     QESDVKFVNRKRERRGEPLIEPLYTQADAAEVFPLMVPTPYGQEFEPINGVTAHLVDA
                     GHILGSSAVVLDVQENGRRFRFWFSGDIGRLNLPLLKDPVLPERVDYLMMECTYGDKP
                     HADPQAAYDELRTVCRSAIERSGKIIIPAFAVGRTQELVYYFNQMMTDGEIPKVPVYV
                     DSPLAVNTTRVFQEYPDLYDEETWQFIRENRHPALNFPQLTYTQSADESKALNDKPGP
                     MIIISASGMAETGRILHHLKNNIENPRNTVLIVGWQAPDTLGRRLAERAPLVKIFGET
                     YQRRAEVVTIGGLSAHAGQNMLMQYARASQSQLRKIILIHGEPTPALALMEKFKTEQM
                     PSAAYPEIGETLEI"
     gene            3820..3910
                     /locus_tag="PKL11_09940"
     tRNA            3820..3910
                     /locus_tag="PKL11_09940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3854..3856,aa:Ser,seq:cga)
BASE COUNT          847 a         1292 c          983 g          825 t
ORIGIN      
        1 gggcgggctg tgttataatt tctgtgccga aggtgggaat cgaacccaca ctccttacgg
       61 aacacgattt tgagtcgtgc gcgtctgcca attccgccac tccggcgtaa cattcgcagt
      121 tccaaactgc ctccctagta tatccatctt tttgcgaagg gtcaagagaa acaatgcgac
      181 ttggaattat cggcctcccc caatcaggta aaaccaccct gttcaatgcc cttacccgcg
      241 gcacccagcc caccagcccg gcctccgggc ggattgaagt tcataccgcg gttgttgatg
      301 tccccgaccc gcgcgtggac cggctctcag ccatgttcaa gcccaagaaa accatctacg
      361 ccaaagtaac ctacgccgat attgccggcc ttgatggctc tgctggtaaa agcggcatct
      421 cgggcgtttt actgaatcaa ctcacccaga tggatggcct gatccacgtg gtgcgctgct
      481 ttgaagatga catggtgccg cacccccagg acaccattga ccccatccgg gatatcgcca
      541 ccatggacag cgagttcatc ctcaacgacc tgattgccgt ggagcgcaaa ctggaacgcc
      601 tgaccgaaga acgcaaaaag ggcgctggcc gcgataaatc catcatcgag cgtgaaacca
      661 ccctgtttga acgctttcag gcggctttga gcgctgaaac cccgcttagg gatgtgacta
      721 ttaccgccga agaagagaaa atgctctccg gttttggctt tctcagccgc aaaccgattc
      781 tgatcgtcct caacctctcc gaagggcagc gcccacccga aatctcctac gcccaccaga
      841 gcagtcaaat cgtggcgctg caggcccgcc ttgaagccga cattgcccaa ctgccccccg
      901 atgaggcgca ggttttcctg gcagaatacg gcattgagga accctcgctc aatcgcatga
      961 ttcgcctgtc ctacgacctg ctgggattgc agtccttctt caccgtcggc cccgatgaag
     1021 tccgtgcctg gacggttcag cgcggcgcca ccgccccgga agctgccggc gtcatccaca
     1081 ccgacctgca aaaaggtttc atccgcgccg aggtcgctgc ctatgacgac ctgatcacgc
     1141 ttggcggcct caacgaggcc aaaacccacg gccgcctgcg cttggagggc aaggaataca
     1201 ttgttaaaga tggggatatc ttgaatatcc gctttaacct ctaaccctga ttcatggaca
     1261 ccgcagccct ttcgaagccc atctggattg atcgtcgtca ggccctggaa cgcctggcgg
     1321 ctgaacttct tcgccatacc caggttgctg tggatacgga atcaaacagc ctgtttgcct
     1381 atcaagaaca ggtctgtctg attcagtttt caacccccac tgccgactac ctgcttgacc
     1441 cgctggctct ccccgacctc tccccgctgg cacccctgtt tgccaacccc gcaattgaga
     1501 aaatcttcca tgcagccgaa tatgatctca tctgcctcaa acgcgacttc gattttacct
     1561 ttgccaacat atttgacacc atgcaggccg cccgcatcct gggcaaaacg gcctttggcc
     1621 tgggcgccat cctggaagaa tcttttggca tcacattgaa caaacgctac cagcgggcca
     1681 actggggcga gcgcccgctc tcggcgccca tgctcgatta tgcccgcatg gacacccatt
     1741 acctcatccc gctccgcgcc cgcctggccg cagagttgga gacttctggg cgcaagccca
     1801 tggcccagga agattttacc cggctctgcg ctgtggaggc gggttcactt catgagaatg
     1861 ggcaggactg gtggcgcgtc tccggcgggc gagaccttac accccagcag gcggctgttc
     1921 ttcaagccct gtgcgattat cgcgaccagc aggcgcgcaa cgccaacctg ccgcgcttca
     1981 aagtcttaaa cgatgatgtc ttaatcaccc tggccgaaac ctgcccgcaa accctggcag
     2041 aactgaagcc catcccccac cttggcccgc gcaatatcga gaaacacggc gtccggctgc
     2101 tggcagccat tcagcgtggc ctcaagtcgc ccccgcttca ccgcccttct catccccgcc
     2161 ccgatgatgc ctacctgttc cgcctggatc tgctgcgcga atggcgcaaa gtgactggcc
     2221 gtgaactggg cgtggattct gatgtcattc tgccgcgcga taccatggaa cacatcgcgc
     2281 aggccaatcc tgctaccctg ccagaattgc aggatcaaat gccgctcttg ccctggcgtt
     2341 tcaatcgctt tggcgcagag atcctccgag tcttaccccc aaaggtgaaa tcatgaaact
     2401 gacaatctat ggcgctggtc aaaccgttac cggttcgcag tatgtgttgg aagtgaatgg
     2461 tcaaaccctg cttttggaat gcggtcttta tcaaggtcat cgcagcgaca ccttcgccaa
     2521 aaacctcaat tttgagttca gcccgcgcac agtggatgca gccatcctct cccacgccca
     2581 tattgaccat tcgggcaacc tgcccaacct ggtcaagcag gggtactcgg ggcccatcta
     2641 cgtcacgccc gccaccgccg gcctttgcga attgatgctc atggattctg cccacatcca
     2701 ggaatccgat gtcaagtttg tcaaccgcaa acgcgagcgc cgtggtgagc cgttgatcga
     2761 accgctttat acccaggccg atgctgcgga agtcttcccg ttgatggttc caacccccta
     2821 cgggcaggaa tttgaaccca ttaacggcgt taccgctcac ctggtagatg caggtcacat
     2881 tttgggctct tctgccgtgg tgctggatgt tcaggaaaat ggccggcgct tccgcttctg
     2941 gttctccggg gatattggcc gcctcaatct gccgctgctc aaagaccccg ttctgccaga
     3001 acgggtagat tacttgatga tggaatgtac ctacggcgat aaaccccacg ccgaccccca
     3061 ggccgcctat gacgagctgc gcactgtctg ccgctctgcc atcgagcgca gtggcaaaat
     3121 tatcatcccc gcctttgccg tgggccgcac gcaagaactg gtctattact tcaaccaaat
     3181 gatgactgat ggcgaaatcc ccaaggtccc cgtctatgtg gacagcccgc tggcagtgaa
     3241 caccacccgc gtcttccagg aataccctga cctgtatgac gaggaaacct ggcagttcat
     3301 ccgtgaaaac cggcatccgg cgcttaactt cccgcagctc acctacaccc agtcggccga
     3361 cgaatccaaa gccctcaacg ataaacccgg ccccatgatt atcatctcgg cctcaggcat
     3421 ggctgaaacg ggacgcatcc tccaccacct caagaacaat attgagaacc cccgcaatac
     3481 cgttctaatc gtcggctggc aggcgcccga tacactgggc cgccgcctgg cagagcgcgc
     3541 ccccctggtc aaaatcttcg gcgaaaccta ccagcgccgc gccgaggtgg tcaccattgg
     3601 cggcctctca gctcacgccg ggcagaacat gctgatgcag tacgcccgtg catctcaatc
     3661 tcagcttcgc aaaatcattc tgatccacgg cgagcccacc ccggccctgg ccttaatgga
     3721 aaaattcaaa accgagcaaa tgccttcggc tgcctatccc gagattggcg aaacattgga
     3781 gatttagcct cactctgtgc tataataccc cctgcccgtg gagaggtgcc ggagcggccg
     3841 atcggggcgg tctcgaaaac cgttgtgggg cttgtcccca ccgtgggttc gaatcccacc
     3901 ctctccgcca gtcaagactt cctttgcaaa aagggagtct ttttatt
//
LOCUS       DAOPVS010000169         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_23502_length_3907_cov_45.672118, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000169 DAOPVS010000000
VERSION     DAOPVS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23502_length_3907_cov_45.672118"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKL11_09945"
     CDS             <1..317
                     /locus_tag="PKL11_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ13961.1"
                     /translation="SAGERQRLALARLLLQNAPIWILDEPTANLDALTEQALIQTLLA
                     LSAGHTVIWITHRLIGLDALDEIIALDAGIVMERGRHADLIAADGLYAHLWHLQNRQI
                     AS"
     gene            735..1400
                     /locus_tag="PKL11_09950"
     CDS             735..1400
                     /locus_tag="PKL11_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNZ13962.1"
                     /translation="MDQKITALKVQKRNPNRVNVYLDGDFAFGLARVVAAWLQIGQIL
                     TPEKITSLQAQDTHEVAYQKALQLIAIRPRSTAEIQQRLTRHGFDPAVIEATLERLKE
                     NRLLDDAAFARLWVENRSTFRPRGRRVLALELRQKGIAADAIDDALDESVDDNALALQ
                     AAERYARRLQDLDWMAFRTRLSAFLGRRGFDYQTTAEVVRQVWVSHHTLETLNTDLEI
                     EEN"
     gene            1403..2950
                     /gene="rny"
                     /locus_tag="PKL11_09955"
     CDS             1403..2950
                     /gene="rny"
                     /locus_tag="PKL11_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNZ13963.1"
                     /translation="MEVLGYILSGVGVLAIIAAVIIANVLSKTKIDNFKQQFYVEQQN
                     KADNIVLVANERAKTIELEARDKSLRLQQDAESELTRRRSEIVHEEERLQKRRTELDH
                     RTERMELREQAINKRQSALDKRANDIEKMAVDKLAELQKVANMSVDEARAQLLQEVEK
                     ESRDDMARIIRQIEAEARADGEKRAREIVADAIQRVASDHVAAVSTSVVPLPNEEMKG
                     RIVGRNGRNIRAFEQAAGVDVIVDDTPEAVTISCFDPVRREVARRSLERLIVDGRIHP
                     AHIEKILNDTQREVDKDIVEAGEQAVFEAGVAGLHPEIIRVLGRLRFRTSYGQNQLAH
                     AVEVARLSAVIAADLGADVDISRAAGLLHDLGKAMDHNVDGTHASIGAEFAKRYGVHP
                     RVVNAIAAHHHEVEQESVEAVIAEAADAISGARPGARREDLEQYIKRLRALEDIATSF
                     KGVSQSYAIQAGREVRIIVRPEDIDDLAATRLARDIAKKIEETMQYPGQIKVTVIRET
                     RAVEYAK"
     gene            complement(3024..>3907)
                     /locus_tag="PKL11_09960"
     CDS             complement(3024..>3907)
                     /locus_tag="PKL11_09960"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNZ13964.1"
                     /translation="VPTFHKLAADQFEARIRIYSETLLRGLEGVDPALQSRRQKALAN
                     IQQVILRRGATGAMRWVSTMYPTPAYAENAGMDLETYRDFFYRACHADAQTTEPVAYW
                     QNKREEQKKHIARIEGHGLVTLKGPNVDLSLSILGRKFNNSSGYHNLPDGEIYTGPVE
                     DSANGWVKFTYPAMYGGRAVEGVELRFEKGRVVEATAKSNQDFLHEMLETDSGARYLG
                     EFAIGTNFEIDRYTGNILFDEKIGGTFHLALGMGYPETGSMNRSAIHWDMICDMRTDS
                     EIRVDGELFYRNGAFAF"
BASE COUNT          876 a         1094 c         1032 g          905 t
ORIGIN      
        1 tgagcgctgg tgagcgccag cggcttgcac tggcacgcct gcttttgcag aatgccccca
       61 tctggatatt ggacgaaccc actgccaatc tggatgctct gaccgagcag gcgctcatcc
      121 agactctgct ggcgctttct gccggtcata ccgtgatttg gattacacac cgcctgattg
      181 ggctggacgc tcttgatgaa atcatcgctc ttgatgccgg catcgtcatg gaacgcggcc
      241 gccacgccga tttgattgca gccgacggcc tctacgccca tctctggcat ctgcaaaacc
      301 gccagatagc atcataaaaa cctgggcaat aaccctttat tttggtttgc ccttttatga
      361 aaatcatgta taatatgttt caagattgtt tcacagcttc cctggacgtt cagaagagcg
      421 ttggagctag aagtcaatcc gtgtttgttg ggcatagtta tctcccgcaa gggagttgca
      481 acagtctgat gatccttgtt caggtgatca gattgcaaag ctgaaggaca tttctcgcaa
      541 aatgacgaca ctatcggcat taaatgcatc aatccagcag ccacccttga agcggcggat
      601 tattccgctg gtggacctgc cttaatttcc ttcagtcagc gtcttttaca ggaatcggtt
      661 cgttagtttt accagcatcc acgtgacttg acttgtagcc accattatcc gggggacgga
      721 tagatgacgg tcgcatggat cagaaaataa cggcgcttaa agttcaaaaa cgcaatccca
      781 accgggttaa tgtgtacctg gatggtgatt ttgcttttgg cttggcccgg gttgttgcag
      841 cttggctgca aattggtcag atcctgaccc ctgaaaaaat cacctcgctg caggctcaag
      901 atacccatga agtggcctat caaaaagcgc tgcagttgat tgctatccgc cctcgcagca
      961 ccgccgaaat tcagcagcgg ctcacacgcc atggttttga tcctgccgtg atcgaagcca
     1021 cccttgagcg tctcaaagaa aaccgtttgc tggatgatgc ggcttttgcc cgtctctggg
     1081 ttgagaaccg ctcaacgttc cgcccgcgtg gacggcgtgt gctcgccctg gagcttcgcc
     1141 agaagggaat cgctgccgat gcgattgatg acgcgctcga tgagtccgtg gacgataacg
     1201 ccctggcttt acaggccgct gagcgctatg cccggcgctt gcaggatctc gattggatgg
     1261 catttcgtac ccggctttcg gcctttttgg gccgccgggg atttgattat cagacgaccg
     1321 ccgaggtagt tcgtcaggtc tgggtcagtc atcatacgtt ggaaacgcta aatactgatt
     1381 tggaaattga ggaaaattaa aaatggaagt actgggatat atcttatccg gtgttggcgt
     1441 gttggcgata attgctgctg taattattgc caacgtcctg tccaaaacca aaattgataa
     1501 cttcaagcag cagttctatg ttgaacagca aaacaaagcc gacaacatcg ttctggttgc
     1561 gaacgagcgg gccaaaacca ttgagttgga agcccgcgac aaatccctgc ggttacagca
     1621 ggatgctgag tcagaattga ctcgccgccg cagcgaaatt gttcacgagg aagagcgcct
     1681 ccaaaaacgc cgcactgagc tggaccaccg cactgagcgg atggaactgc gtgagcaggc
     1741 gattaacaag cgccagagcg ccttggataa gcgcgccaat gatattgaaa aaatggctgt
     1801 ggacaaactg gctgaactgc agaaagttgc caacatgtct gttgacgaag cccgcgctca
     1861 gttgttgcag gaagtcgaaa aagaatcccg cgatgacatg gcccgcatca tccgccagat
     1921 cgaggccgaa gctcgcgccg atggcgaaaa acgcgcccgt gagatcgttg ccgacgccat
     1981 tcagcgcgtg gcctctgacc acgttgccgc agtttcaacc tcagttgtcc ccctgcccaa
     2041 tgaggaaatg aagggccgca tcgtgggccg caatggccgt aacatccgcg cctttgaaca
     2101 ggctgccggt gtggacgtga ttgtggatga cacgcccgag gcagtgacga tatcctgctt
     2161 tgatcccgtc cgccgcgagg tcgctcgccg ctccctggag cgcctgattg tggatggccg
     2221 cattcaccct gcccacattg aaaaaattct caacgacacc cagcgcgagg tggacaagga
     2281 tattgttgaa gctggcgaac aggctgtctt tgaagctggc gttgctggtc tgcatcccga
     2341 aatcatccgg gtcttgggtc ggctccgctt ccgcacatcc tacgggcaga accagttggc
     2401 tcatgccgtt gaggttgccc gcctttctgc cgtcatcgct gctgacctgg gcgccgatgt
     2461 ggatatttct cgtgccgctg gcctgctcca tgacctgggc aaggccatgg atcacaacgt
     2521 ggacggcacc cacgcttcaa ttggcgctga atttgccaaa cgttatggcg ttcacccccg
     2581 tgtagtcaat gccattgctg cccaccacca cgaagttgag caggaaagcg ttgaagccgt
     2641 catcgccgaa gctgccgatg ccatctccgg cgctcgccct ggcgcccgcc gcgaagacct
     2701 ggagcagtac atcaagcgcc tgcgcgcact cgaagatatt gccacctcct tcaagggcgt
     2761 cagccagagc tacgctattc aggctggccg cgaagtccgc attatcgtcc gtccggaaga
     2821 tattgatgat ttagccgcca cccgtctggc gcgtgatatt gccaagaaga ttgaagaaac
     2881 catgcaatac cccggtcaaa tcaaggtaac cgtcattcgc gaaacccgtg ccgtggaata
     2941 cgccaaatag cggcttaccg gtccggtaaa tcaaaaggga cggattattc tccgtccctt
     3001 tttgatttca gttttagtcg aagctaaaag gcaaacgccc cgtttcggta gaacagctcg
     3061 ccatccaccc gaatttccga atccgtccgc atgtcgcaga tcatatccca gtgaatcgcg
     3121 ctgcgattca tactgcccgt ctcggggtag cccataccaa gcgccagatg gaacgtcccg
     3181 ccgatttttt catcgaataa gatattaccg gtgtaccggt caatctcaaa attggttcca
     3241 atcgcaaatt cgcccagata gcgggcgccg gaatcggtct ccagcatttc gtgcaagaag
     3301 tcctgattgc ttttggcggt tgcctcaacc acgcggcctt tttcaaagcg caattccacg
     3361 ccttcaaccg cgcgcccgcc gtacatcgcc gggtaagtga acttaaccca gccattagcc
     3421 gaatcttcca caggtccggt ataaatttca ccatctggca ggttgtgata tccgctggag
     3481 ttgttgaact tccgccccag aatggagagc gacagatcta cgtttggtcc ctttagtgtg
     3541 accagaccgt gcccctcaat ccgggcaata tgcttcttct gttcttcgcg tttgttttgc
     3601 cagtaggcca ccggttcagt ggtttgcgca tcggcatggc aggcccggta gaagaaatcg
     3661 cggtacgttt ccaaatccat acccgcattt tcggcatagg ccggggtggg gtacatggtg
     3721 cttacccagc gcatggcgcc agtggcgccg cgccgtaaaa tcacttgctg aatatttgcc
     3781 agcgccttct gccgccggga ttgcagcgcc ggatcaacgc cttccagccc gcgtaagagc
     3841 gtttcagaat aaatccggat gcgggcttca aattgatccg ctgccagctt gtggaatgtc
     3901 ggcacaa
//
LOCUS       DAOPVS010000170         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_23773_length_3873_cov_63.079361, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000170 DAOPVS010000000
VERSION     DAOPVS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23773_length_3873_cov_63.079361"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1160
                     /locus_tag="PKL11_09965"
     CDS             30..1160
                     /locus_tag="PKL11_09965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ13965.1"
                     /translation="MPDPEMVTVAPSTTPSSQFQVYIYGDPATVEAGWTRSRDIEGNA
                     MWYRRSTYNGQAVAASYLPPLTAPGKYRLQVFVPDNHATIRDAMYFVVDHKTAVRREI
                     KCVVNQNIYRNQWVELSGTEGPGQPLVNVFELDPVFDDSGRVNLVDTTFINELNRPKG
                     FEIAFSAIRWVPISTPDPIHDEGDMAGVFDAPVGTDAARRNPVLGAPGNVIYIGGQPF
                     PIWLDEWFDYNTLGSRYLLRKGVYAIHTGADLNRMGGALADKDQPVYACADGVVRYSG
                     SLSSGWKGVVLIEHEVPGEAPVYIRYGHLYSLRFQNGARVKRGEIIGLITEYAPYNYH
                     LHFDITRNPAIVSNVGHWPGDNYNAVMSFYIDPLEFLRAHHR"
     gene            1700..3034
                     /gene="dnaA"
                     /locus_tag="PKL11_09970"
     CDS             1700..3034
                     /gene="dnaA"
                     /locus_tag="PKL11_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNZ13966.1"
                     /translation="MNAQQAWEATLGQLQTEMSKAAFDTWVRPAALIGAEGDQLTIGV
                     QNAYARDWLESRLQDSVQKLLSNFLVRPVQVRFVVWHKDYDQEPEALEDIEPEAPAGR
                     PCSQFNARYVFDNFVVGGSNRMAHAACSAVAENPAHAYNPLFLYGGVGLGKTHLLHAI
                     GNAVCQRGLQVLYVSSEEFTNDLINAIRTHNTPAFRDRYRNIDVLLIDDIQFIAGKES
                     TQEEFFHTFNALHGQEKQIVLTSDRPPKAMVTLEERLRSRFEWGLIVDIQPPDLETRQ
                     AILRSKAERAGRATPDAIIEAIARQVQSNIRELEGALTRVLAYADLSGRPLTVELVDI
                     ALADLLPQRRSLEPRQVVEIVASAFGVTFEQMIGRDRSRDVALPRQVAMYLLREAINV
                     SLPQIGEALGGRDHTTVMYACEKVADLIESDDRLRRQVVQIREQLVGRTARV"
     gene            3103..>3873
                     /locus_tag="PKL11_09975"
     CDS             3103..>3873
                     /locus_tag="PKL11_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ13967.1"
                     /translation="MSPLIKPSTRAFFREARRMSGYPLSEWLHGYFYLRYTYAYIGNA
                     MGLRRPARFYLWLLRLWRRIFPPRPRAPRPGAITFADTYHGKVVPLETARQLVQVHEP
                     IRISYPEQVLPYALARDLILQQPDHIVALDCPCRSARPNPCLPLDVCLIVGEPFASLV
                     MEHHADRAHWITPQTAVAILQAEEQRGHVHHAFFKQAVLGRFYAICNCCACCCGAMQA
                     HRNGTPMLASSGYLCRVSAADCAGCGACAAVCPFGALEI"
BASE COUNT          708 a         1271 c         1121 g          773 t
ORIGIN      
        1 ttttccccat ttttctcgga ggaatcaata tgcctgatcc tgagatggta accgttgccc
       61 ccagcaccac ccccagcagc cagttccagg tctatatcta tggcgacccg gcaactgttg
      121 aagcgggctg gacgcgctcg cgtgatattg aaggcaatgc catgtggtac cgccgcagca
      181 cctataacgg tcaggcggtg gctgccagct acctgccccc cctgaccgcc cccggcaaat
      241 accgcctgca ggtctttgtg cccgataacc acgccaccat tcgcgatgcg atgtactttg
      301 tggtggatca caaaacggcc gtccgccgcg agatcaaatg cgtggtcaat cagaacatct
      361 accgcaacca gtgggtggaa ctgagcggca ccgaaggccc cgggcagccc ctggtcaacg
      421 tctttgagct ggacccggtt tttgacgaca gcggccgcgt gaacctggtg gataccacct
      481 ttatcaacga actcaaccgc cccaaagggt ttgagattgc cttcagcgcc atccgctggg
      541 tgccgatttc gacccccgac cccatccatg atgaaggcga catggccggc gtgtttgatg
      601 cccccgtggg caccgatgcc gcccgccgca accccgtgct gggcgcaccg ggcaacgtca
      661 tctacattgg cgggcagccc ttccccatct ggctggacga gtggttcgac tataacacgc
      721 tcggctcgcg ctacctgctg cgcaagggcg tctatgccat tcacaccggc gccgatctca
      781 accgcatggg cggcgccctg gccgataaag accagccggt ctatgcctgc gccgatggcg
      841 tggtgcgcta ttcgggctcg ctgagcagcg gctggaaggg cgtggtgctg atcgagcatg
      901 aagtgcccgg cgaggccccg gtctatatcc gctacgggca cctttacagc ctgcgcttcc
      961 agaacggcgc gcgggtcaag cgcggcgaaa tcatcgggct gatcaccgaa tacgcgccct
     1021 acaactacca cctgcacttt gatatcaccc gcaacccggc catcgtcagc aatgtgggcc
     1081 actggcccgg cgataactac aacgccgtga tgtccttcta cattgacccg ctcgaattcc
     1141 tgcgggcgca tcaccgctaa ccccgccggt tttgaatcac cgcggcgctc cggctgaccc
     1201 ccggagcgcc gctttttgcc ccgtgaaaca agattcacat ttcggcatta gaatcacacc
     1261 gcgccgcagg ttaacgagtc gttaaaactt ttggaaaacc catccatata tgccactgaa
     1321 ggccctacta agtgccgacc ccctatatat gggggtgtct gaacgccgcc agcggcacta
     1381 gtggaaagtc tttcaaaccc tgaacattaa agtagtttgc gttctttcgc catttcttta
     1441 aggttggcaa ccttaaatat tcgaatcttt ttaagctggc gtagggttcg cgccttgttt
     1501 ggcaggcccg tttctgctag aatctaacca atccagtcgg ggcaaatgcc cttgtaagtg
     1561 actacccggg tctgacgacc cttaccgttt gattattgtt aaacccagtc tgtacgtccc
     1621 actgctcgcc ggcagtgggt ttctgtccct gttgcctgca cctgtgccgg tgccattcct
     1681 tttgaagaga ggtcgctgca tgaacgctca acaagcctgg gaagccaccc tcggacaact
     1741 gcaaactgaa atgtcgaaag ccgcctttga tacctgggtg cgcccggccg cgctgattgg
     1801 tgcggagggg gatcaactga ccattggcgt gcaaaacgcc tacgcccgcg actggctgga
     1861 aagccgcctg caagacagtg tgcagaagct gttgagtaat tttttggtgc gcccggtgca
     1921 ggtgcgcttt gtggtctggc ataaagatta tgaccaggag cccgaggccc tggaagacat
     1981 tgagcccgag gcgccggctg gccgcccctg ctcacagttt aacgcccgct atgtgttcga
     2041 taattttgtg gtgggcggca gcaaccgcat ggcgcacgcc gcctgcagcg ccgtggccga
     2101 aaaccccgcc catgcctaca atccgctgtt cctctacggc ggcgtggggc tgggcaagac
     2161 ccatctgctg cacgccattg gcaatgccgt ctgccagcgc gggctgcagg tgctgtacgt
     2221 ctcttcggaa gaatttacca acgacctgat caacgccatc cgcacgcaca acacccccgc
     2281 cttccgagac cgctaccgca atattgacgt gctgctgatt gacgatattc aattcatcgc
     2341 tggcaaagaa tcgacccagg aagaattttt tcacaccttc aacgccctgc acgggcagga
     2401 aaagcagatt gtgctgacct cggaccgccc gcccaaggcc atggtgacgc tggaagaacg
     2461 gctgcgctct cgctttgagt ggggtctgat tgtggatatt cagccgcccg acctcgagac
     2521 ccgccaggcc atcctgcgct ccaaggccga gcgcgccggg cgcgctacgc ccgatgccat
     2581 catcgaggcg attgcccgcc aggtgcagtc caacatccgc gagctggaag gcgccctgac
     2641 ccgcgtgctg gcctacgccg acctgagcgg ccgcccgctg accgtggaac tggtggacat
     2701 tgccctggca gacctgctgc cccagcgccg ctcgctggag ccccgccagg tggtggagat
     2761 tgtggccagc gcctttggcg tgacctttga gcagatgatc gggcgcgacc gctcgcgtga
     2821 cgtggcgctg ccccgccagg ttgccatgta cctgctgcgc gaggcgatca acgtttcgct
     2881 gccgcaaatt ggcgaagccc tgggcgggcg cgatcacacc acggtgatgt acgcctgtga
     2941 aaaagtggcc gacctgatcg agagcgacga ccgcctgcgc cgccaggtgg tgcagattcg
     3001 ggagcagttg gtggggcgga ccgcccgggt ttaggctgtt ctcaacccct caaaagcagg
     3061 cgggcgcaca gcccgccttt tgatgtaaaa taggggtgcc ccatgtctcc cttaatcaag
     3121 ccttcaaccc gcgccttttt ccgtgaggcc cggcggatgt ccggttatcc gctgagcgag
     3181 tggctgcacg gctattttta cctgcgctac acctacgcct atattggcaa tgccatgggc
     3241 ctgcgccgcc cggcgcgctt ctacctctgg ctgctgcggc tctggcggcg catcttcccg
     3301 ccgcggccgc gggccccgcg ccccggcgcc atcacctttg ccgataccta tcacggcaag
     3361 gtggtgcccc tggagaccgc ccgccaactg gtgcaggtgc acgagcccat ccgcatcagc
     3421 taccccgagc aggtgctgcc ctacgccctc gcgcgtgacc tcatcctgca gcagcccgat
     3481 cacattgtgg cgctggactg cccctgccgg tcagcccgcc ccaacccctg cctgccgctg
     3541 gatgtctgcc tgattgtcgg cgagccgttt gcctcgctgg tgatggaaca tcacgcagac
     3601 cgggcgcact ggattacgcc gcagaccgca gtggcgatcc tgcaagccga agagcagcgc
     3661 gggcatgtgc atcatgcctt tttcaagcag gcggtgctgg ggcggtttta tgccatttgc
     3721 aactgctgcg cgtgctgctg cggcgccatg caggcgcacc gcaacggcac ccccatgctg
     3781 gcatcttcgg gctacctgtg ccgggtcagc gccgccgact gcgccggctg cggcgcctgt
     3841 gcggcggtct gcccgtttgg cgcgctggaa att
//
LOCUS       DAOPVS010000171         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_24004_length_3846_cov_48.117911, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000171 DAOPVS010000000
VERSION     DAOPVS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24004_length_3846_cov_48.117911"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1638)
                     /locus_tag="PKL11_09980"
     CDS             complement(262..1638)
                     /locus_tag="PKL11_09980"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNZ13968.1"
                     /translation="MPIHPPEPFRIKMVEPIRLISPEARQAAMVAAGHNVFTLHAEDV
                     FIDLLTDSGTGAMSQEQWAAMMRGDESYAGARSYLRLADTMQQIFGFEHFVPTHQGRA
                     AENIISALLIQPGMVVPSNMHFDTTDGNIRARGGRPVNLVQDVAFDPEARVDFKGNMD
                     IDKLRAFIEETGVQNIPFGAITITNNAGGGQPVSLANIRAVSEVYHHYGIPFFIDCCR
                     YAENAYFIKLREPGYANKSTLEIAREIFSLADGAWMSAKKDGMVNMGGFLAVRDAELY
                     QKICNELIMREGFVTYGGLSGRDLDALATGLWEGLDEAYLAYRLGQTAYLAEGLNAVG
                     VPTIQPPGGHAVYIDAGQLLPHIPHAEFPGQALTAALYLEGGIRGVEIGSVMFAYPNP
                     DTGEMVYPKLELVRLAIPRRVYTQSHMDYVIETAAKLTQKAAGLRGYRITYAAKLLRH
                     FTARFEPL"
     gene            1892..>2520
                     /locus_tag="PKL11_09985"
     CDS             1892..>2520
                     /locus_tag="PKL11_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13969.1"
                     /translation="MAVQIYPASDTTVLRINVSGDLFISGWDQPRVRVAADDEDGLVA
                     QQSGDDLQIIAPDDLVLMVPAAVTLQIGHAGGDVELKDLTGNVTLSQAGGDLTAQRLN
                     NLTLGAVGGDVELRQINGRLSAGQIGGDMDAVSLQDLAEVQQVGGDVALHLLAGGANI
                     RSGDDVRVSLAQPAGQTLNIHAGGSVRLYLPTDAGATFDLTSGDEDICI"
     assembly_gap    2521..2620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2621..3150
                     /locus_tag="PKL11_09990"
     CDS             <2621..3150
                     /locus_tag="PKL11_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13970.1"
                     /translation="DAGATFDLTSGDEDICIELGDKDVAVEEGLYHGTVGDGAIHVRI
                     AAGEDILITDRRPDIDSYTRIFDRWDERWERVMERMNLQERLREEAMRRAQEAAERAG
                     RRAEEAARRAEERVQATMRRLESRRGGIPTPPASAGSRVSRDEKLLILQMLQEQKITA
                     EEAERLMQTLENLSE"
     gene            3191..>3846
                     /locus_tag="PKL11_09995"
     CDS             3191..>3846
                     /locus_tag="PKL11_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13971.1"
                     /translation="MNETPDRLEILRQVERGELTPQQAAQLLGLDEPLSDPEPEPAAN
                     FETRETPPPWPTAVEPPIESSESSSTSEFELGRFKFWRRLSALPFWFSVAITALGAYW
                     MYSGWQAAGFGWGFFLSWIPLLLGVLGMLLFWQTRWLHLRVRQGHGKRPEVIRISLPL
                     PLTAGAWFLRSFSGVLPADLRDKHLDEMVEGLGKSVSGDDPIHIYVDDEEDGDQVEIY
                     "
BASE COUNT          816 a         1083 c         1053 g          794 t
ORIGIN      
        1 aagccagcac ggcgtcgcgg tcttcaccgg tcagggtggt gccgctgctg caggctgcca
       61 gtgacagcac cagagcaaaa agtaaagcag aacgtaacag ggttttcata tcattgtcct
      121 ttcaggctgg cggctcaaca gccgccaaaa ttgtatcatt gtatcattta taatcctgat
      181 taggcggcct gtcaagactt ataaaaccag agtgcgggca gtctgcccgc actctggtta
      241 tttatttggg ttgatgtcgc cttacaaggg ctcaaaccgt gctgtaaagt gccgcagcag
      301 cttggccgca taggtaatcc ggtacccgcg cagtccggcg gctttttgcg tcagctttgc
      361 agcagtctca atcacataat ccatgtgact ctgtgtgtac acccggcggg ggatcgccag
      421 ccgcaccagc tccagcttgg ggtacaccat ctcgcccgtg tccgggttgg ggtaagcgaa
      481 catcaccgag ccaatctcca cgccccggat gccgccttcc aggtaaagcg ccgcagtcag
      541 cgcctgtccg gggaactcgg catgtggaat gtgcggcaga agctgcccgg catcaatgta
      601 aaccgcgtgc ccgcccggcg gctggatggt cggcacaccg actgcgttca gcccttcagc
      661 caggtaggcg gtttgaccaa gccggtaggc caggtaagcc tcgtccagcc cttcccacaa
      721 acccgttgcc agcgcatcca ggtcgcgccc cgaaagcccg ccgtaagtga caaagccttc
      781 ccgcatgatc aattcattgc aaattttctg gtacagctca gcatcgcgca cggccaaaaa
      841 gccacccatg ttgaccatgc catctttctt ggcgctcatc caggccccat cggccaggct
      901 gaagatctcg cgggcaattt ccagcgtgga tttgttcgca tatcccggct cgcgcagctt
      961 gatgaagtac gcattttcgg cataccggca gcagtcaata aagaagggga tgccgtagtg
     1021 atgatagact tcagataccg cccggatatt cgccagcgaa accggctgac cgccgccagc
     1081 gttgttggta atggtgatcg ctccaaaggg gatattctgc acgccggttt cttcgatgaa
     1141 tgcgcgcagc ttgtcaatat ccatgttgcc tttgaaatcc acccgggctt ccgggtcaaa
     1201 ggccacatcc tgaaccaggt tcaccgggcg cccgccgcgg gcgcgaatat tgccgtcagt
     1261 ggtgtcaaaa tgcatattgg aaggcaccac catccccggc tgaatcaaca gcgcagagat
     1321 gatattctcg gccgcgcggc cctggtgagt gggcacaaag tgttcaaagc cgaaaatctg
     1381 ctgcatggta tccgccaggc gcaaataaga gcgcgccccg gcataggatt catcgccgcg
     1441 catcatcgca gcccactgct cctggctcat ggcgccggtg cccgagtcgg tcagcaaatc
     1501 aatgaagacg tcttcagcgt gcagcgtaaa gacattatgc ccggcagcca ccatggcagc
     1561 ctgcctggct tcgggtgaaa tcaaacggat gggttccacc atcttgatac ggaagggttc
     1621 tggggggtga atgggcatag gttctcctct ctaagtttta ttagtgtagc accaaacccg
     1681 gctctggcga agtgaaaagt gtcccttctc tattgttggg tgtcattcat ttatctggaa
     1741 tattgacgcc agagacaatt ttccagaaat caaaacaaaa tattgataaa atcaaagtca
     1801 ttataaataa tatcaataat atacttgaca aaatcaaaat atctgctaat attagataaa
     1861 gtaattcccc ttcccttatt ggaggtgctt catggccgtt caaatttacc ctgcaagtga
     1921 tacaaccgtc ctgcggatta atgtcagcgg cgacctgttc atcagcggct gggaccagcc
     1981 ccgggtgcgg gttgcggcag atgacgagga tgggctggta gcgcagcaat ccggcgatga
     2041 tctgcaaatc attgcccccg atgatctcgt gttgatggtc ccggcagccg tgaccctgca
     2101 aattggacac gccggtggtg atgttgaatt gaaagacctg accgggaacg tgaccctcag
     2161 ccaggccggc ggtgatttaa ccgctcagcg cctcaataac ttgaccctcg gcgccgttgg
     2221 cggtgatgtc gagctgcgcc agatcaacgg gcgcctgtct gccgggcaga ttggcggcga
     2281 tatggatgcc gtttcgctgc aagacctggc tgaagtccag caggttggcg gcgacgtggc
     2341 gcttcacctg ctcgctggcg gagccaatat tcgctctggc gatgatgtgc gggtctccct
     2401 ggcacaacct gccggccaaa cgctcaatat tcacgctggc ggcagcgtgc gcctctattt
     2461 gcccaccgat gctggcgcta catttgactt gaccagcggc gatgaagata tttgcattga
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgatgctgg cgctacattt
     2641 gacttgacca gcggcgatga agatatttgc attgagctgg gcgataagga tgtagctgta
     2701 gaagaaggcc tgtatcatgg aacagtcgga gacggcgcca tccatgttcg gattgccgcc
     2761 ggcgaagata ttctgatcac cgaccgccgc cccgatattg acagctacac acggattttt
     2821 gaccgctggg atgagcgctg ggaacgtgtg atggagcgca tgaatctcca ggaacgcctg
     2881 cgagaagaag ccatgcgccg ggcccaggaa gctgccgaac gcgccgggcg ccgggccgaa
     2941 gaagctgccc gccgggctga agagcgcgtc caggcaacca tgcgccgcct cgaatctcgc
     3001 cgcggcggca tccccacgcc gcctgccagc gccgggtcac gcgtcagccg ggatgaaaag
     3061 ctgctgattt tgcaaatgct ccaggagcag aaaattactg ctgaagaagc cgagcgcctg
     3121 atgcaaacac tggaaaactt gagcgaatag ccccatttcg cagtacaatc aatacaggaa
     3181 ttttaaccac atgaacgaaa cccctgaccg tctggaaatt ttgcgccagg tagagcgcgg
     3241 cgaactgacc cctcagcagg ctgcccaact gctgggcctg gatgagccgc tgtctgatcc
     3301 cgaacctgaa ccggctgcca attttgaaac ccgcgaaaca cccccaccct ggcccacagc
     3361 ggttgagcca cccatcgagt cgtccgaatc atcttccacc agcgaatttg aactgggtcg
     3421 cttcaagttc tggcggcggc tctcggcgct gcccttctgg ttctcggtgg caatcacggc
     3481 gcttggcgcc tactggatgt attcgggctg gcaagcggct ggctttggct ggggcttctt
     3541 cctgtcctgg atcccattgc tgctgggcgt gctgggcatg ctgctcttct ggcagacgcg
     3601 ctggcttcat ctgcgtgtcc ggcaggggca tggcaagcgc cccgaagtga ttcgcatcag
     3661 cctgccgctg cccctcacgg caggggcctg gttcctgcgc tcattctccg gcgttctccc
     3721 cgccgatctg cgtgataagc acctcgatga aatggtagaa ggcttgggaa aatccgtctc
     3781 gggcgacgat cccatccaca tctatgtgga tgacgaagaa gatggcgacc aggtcgaaat
     3841 ctacat
//
LOCUS       DAOPVS010000172         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_24239_length_3817_cov_53.190058, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000172 DAOPVS010000000
VERSION     DAOPVS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24239_length_3817_cov_53.190058"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1055)
                     /locus_tag="PKL11_10000"
     CDS             complement(168..1055)
                     /locus_tag="PKL11_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNZ13972.1"
                     /translation="MLKVGYIGLGLMGKSIARNIHKAGFPLVVHNRSRAVVDELVAEG
                     AVAAFSPAEVAAQVDVVFTNLPDSPDVEQVALGPQGIIAGAHPGLIFVDNSTIKPATA
                     RQIAAALEPHGVQCLDAPVSGGDIGARNATLAIMIGGPAAALETVRPVLMAMGKTITH
                     VGESGAGQIAKAANQIMVAAQMVAMGELLIFAQQAGADPRKVIEAIRGGAAQCWTLDV
                     KPPRLFDGNRQPGFKARMQAKDLNIVLETAREYGIPLPSAAVDAQLFNAMIQNGLGDL
                     DNSAVLAMIEALANAPLKI"
     gene            complement(1131..1580)
                     /locus_tag="PKL11_10005"
     CDS             complement(1131..1580)
                     /locus_tag="PKL11_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13973.1"
                     /translation="MTDLRTLFSQDEWKLLQFSALWVFQTVAVADGKIDEGEVRALGR
                     ELSEAHLYHNPLASQTLQTLADDFNAIYKLFKTDERGPRDGLAAVADLVEERMGMDEA
                     RRFKMALLLLGRNIALAEGGGFLGLGERTSEQEKAALVTIASALRTM"
     gene            complement(1577..2347)
                     /locus_tag="PKL11_10010"
     CDS             complement(1577..2347)
                     /locus_tag="PKL11_10010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13974.1"
                     /translation="MTTPSNLQKAAERGEPSHVWRAGQERRLHQIQAAAGDRARGRVF
                     VDGCGLGAYLARLTPAAALAAGLDIEFPRTLDARQNTHTLAGEAAPLLVVCGAGEALP
                     FPSDYFDLILSHEVLEHVQDDRAAIAEMIRTLRPGGRLALYCPNRGYPFETHGIYWQG
                     RYHFGNIPLVNWLPRPWRNRLAPHVRVYSRRDLEALFAGLPVRFVSRTHVFGAYDNII
                     ARRPRLGRALRAFLQALERTPLRILGLSHFWVVEKVTL"
     gene            complement(2331..>3817)
                     /locus_tag="PKL11_10015"
     CDS             complement(2331..>3817)
                     /locus_tag="PKL11_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNZ13975.1"
                     /translation="PPEEIARMAHPPRGLGLIGALMHSHTAIRIPDIAADRRRVGFPK
                     HHPPMRSFLGVPIRHSGRQLGQLYITEKIGAPEFNLQDQQVIEVLAAYAAVAIHNAHL
                     YNNLLERDRALTRRNQNLALLTELASTLASASEIDEILTQSIPPVMDSLHLEVGEVFL
                     RQGESRILSLTLHRSAQVSALWKKSQFMLGEGPVGQVAQTGQPLRFDLQSPEAADLHP
                     DAQAAGLKQMACFPITGRQGPLGVLCIATTHETPLDDLETQFMLNISAWMGTALENAH
                     LSLQSRRLAVLEERERFGMDLHDGVIQSIYGVGLTLEHARLLINQDLPATRQRIEQAI
                     GDLNSTISDIRSYILDLRPRQLHDDNLMQGLERLVHEFQRNRSIPVSLQGPSDGFPNM
                     TDAQALTIFHICQEALANIAKHAHATAVEVVVWKAQDRVLLEVHDNGRGFDPDQTRQR
                     LGHGLSNMLTRALNVGGEVDISSEPGNGSTILAWVPYLPDDDTL"
BASE COUNT          735 a         1085 c         1283 g          714 t
ORIGIN      
        1 tgaatttgcg ttttgattct accacggggc aaggggtaat tttttaattt tagatatgag
       61 attgtgcaaa caattgtcat caggggatat gatgtccacc ccctccctgc ttgaacgggg
      121 agggggtaag aatttatcca agctgtgaaa cccgggctgc cgacaggcta gattttgagc
      181 ggggcattcg ccagtgcttc aatcatcgcc agtacggcag agttatccag gtcgccgagg
      241 ccgttctgaa tcatggcgtt gaagagctgg gcatccacgg cggcggaggg cagcggaatg
      301 ccgtattcgc gggcggtttc gaggacaatg ttgaggtctt tggcctgcat gcgggccttg
      361 aagcctggct ggcgattgcc gtcaaagagg cgcgggggtt ttacgtccag ggtccagcac
      421 tgggcggcgc cgccgcggat ggcctcgatg accttgcggg gatcggcacc ggcctgctgg
      481 gcgaagatca acagctcgcc catggcaacc atctgagcgg cgaccatgat ctggttggcg
      541 gccttggcaa tctgcccggc gcccgattcg cccacatggg taatggtctt gcccatggcc
      601 atcagcaccg ggcggacggt ttccagggcg gcggcggggc cgccgatcat aatcgcgagg
      661 gtggcattgc gggcgccaat atcgccgccg ctgacagggg catcgaggca ctggacgccg
      721 tgcggctcca gcgcagcggc gatctgccgg gcggtggcgg gcttgatggt tgagttgtcc
      781 acaaagatca gaccggggtg agccccggct atgatgccct gcgggcccag cgccacctgc
      841 tccacatcgg gcgaatcagg caggttggtg aagaccacat ccacctgggc ggcgacctct
      901 gctggagaaa aggccgccac cgcgccttcg gcgaccagtt cgtccacaac ggcgcggctg
      961 cggttatgca ccaccagcgg gaaacccgcc ttgtgaatat tgcgagcaat ggacttgccc
     1021 atcagaccca ggccaatata cccgactttc aacatagcag ctccttgttg gttgataaaa
     1081 tagatttgac gaaaatatcc cccgcctgaa acagccgggg gaataatgac ttacatggtc
     1141 cgcagcgcgc tggcgatggt gaccagggcg gctttctcct gctcgctggt gcgctcaccc
     1201 aggcccagaa aaccaccgcc ttctgccagg gcaatgttac gccccagcag caagagggcc
     1261 attttgaaac gccgggcttc gtccatgccc atgcgttctt ccaccaggtc ggccacagcg
     1321 gccaaaccat cccggggacc gcgttcatcg gttttgaaga gcttgtagat ggcgttaaaa
     1381 tcatcggcca gggtttgcag ggtttgggag gccagcgggt tgtgatagag atgcgcctcg
     1441 gagagttcgc gccccagggc gcgcacttca ccctcgtcaa tcttgccatc ggccactgcc
     1501 acggtctgaa aaacccacag cgccgagaat tgaagcagtt tccattcgtc ctgactgaac
     1561 agcgtgcgca gatcggtcat agggtcacct tttccaccac ccagaagtgt gagagtccca
     1621 aaatacgcag gggtgtgcgt tccaacgcct gcaaaaacgc gcgcaaggct cgccccagcc
     1681 gcgggcggcg ggcgatgata ttgtcgtagg caccgaagac gtgggtgcgc gagacaaagc
     1741 gcaccggcag cccggcaaac agcgcctcaa ggtcgcggcg ggaatagacc cgcacgtgcg
     1801 gcgccagccg gttgcgccag gggcgcggca gccagttgac caggggaatg ttaccgaaat
     1861 ggtaacgccc ctgccagtaa atgccatgcg tttcaaaagg atagccgcgg ttgggacagt
     1921 agagcgccag ccgcccaccg gggcgcaatg tgcggatcat ctcggcaatg gcagcgcggt
     1981 catcctgaac atgttccaaa acctcgtggc tgaggatcag gtcaaaataa tcagatggga
     2041 aaggcaaggc ctcgcccgca ccgcaaacca ccagcagggg ggcggcctcg cctgccaggg
     2101 tatgggtgtt ctggcgggca tccagtgtgc gggggaactc aatgtccagg ccggcagcca
     2161 gggcagcggc gggggtcagg cgggccaggt aggcgcccag gccgcagcca tccacaaaga
     2221 cgcggccgcg ggcgcggtca ccggcagccg cttgaatctg gtgcaagcgg cgttcctgcc
     2281 cggcgcgcca gacgtgtgaa ggttcgccgc gctcggcggc cttttgcaaa ttagagggtg
     2341 tcgtcatcgg gtaagtaggg gacccaggct agaatggtcg agccgttgcc cggctcagaa
     2401 gagatatcca cctcgccgcc gacattgagg gcgcgcgtca gcatgtttga caggccgtgc
     2461 cccagccgct ggcgggtctg gtcggggtca aagccgcggc cattgtcgtg cacttcaagc
     2521 aggacgcggt cttgcgcctt ccagaccacc acttcaacgg cggtggcatg ggcgtgtttg
     2581 gcgatgttag ccagggcttc ctggcagatg tggaagatgg tcaacgcctg ggcatcggtc
     2641 atattgggga agccatctga cgggccctgg aggctgaccg ggattgagcg attgcgttga
     2701 aattcgtgta ccaggcgttc caacccctgc atgaggttat cgtcatgaag ctggcggggg
     2761 cgcaggtcga gaatatagga gcggatatcg ctgatggtgc tgttgaggtc accaatggcc
     2821 tgctcaatgc gctggcgggt ggcaggcagg tcttgattga tgagcaggcg ggcgtgttca
     2881 agcgtcagtc caacgccgta gatggactgg atgacgccgt cgtgcaggtc catgccgaag
     2941 cgttcgcgct cttccagcac cgccaggcgg cggctttgca ggctgaggtg ggcgttctcc
     3001 agggcggtgc ccatccaggc gctgatgttg agcataaact gcgtttccag gtcatccaga
     3061 ggggtttcgt gggtggtggc gatgcagagg acacccagcg gaccctgtcg ccccgtgatg
     3121 gggaagcagg ccatctgttt caacccggct gcctgagcat cggggtgcag atcggcagcc
     3181 tctggcgatt gcaggtcaaa ccggaggggt tgcccggtct gcgctacctg ccccacaggg
     3241 ccctctccca gcatgaattg ggattttttc caaagcgccg agacctgggc cgagcgatgc
     3301 agtgtcaggc tgaggatgcg gctttcgccc tgccgcagaa agacctcgcc aacctccagg
     3361 tgcaggctgt ccattaccgg ggggatggat tgggtcagga tttcgtctat ttcagaagca
     3421 gacgccaggg tggaggccag ctcagtgagc agcgccaggt tctggttgcg gcgggtcagg
     3481 gcccggtcac gttcgagcag gttgttgtag agatgggcgt tatgaattgc cacggcggca
     3541 taagctgcca gcacctcaat cacctgctgg tcttgcaggt taaattctgg tgcgccaatt
     3601 ttctcggtga tatagagctg ccccaactga cgcccgctgt gacgaatggg gacgccgagg
     3661 aaggagcgca tggggggatg atgcttgggg aatccgacgc gccgccggtc ggcggcgata
     3721 tcggggatgc gaatggcggt atgcgaatgc atcagggcgc cgatgagccc caggccgcgc
     3781 ggcgggtgcg ccatgcgggc gatttcttcc ggcggca
//
LOCUS       DAOPVS010000173         3785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_24511_length_3785_cov_49.899732, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000173 DAOPVS010000000
VERSION     DAOPVS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24511_length_3785_cov_49.899732"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..609
                     /locus_tag="PKL11_10020"
     CDS             55..609
                     /locus_tag="PKL11_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ13976.1"
                     /translation="MRELSLHLLDIAENSVAARATTIFVVVEENLVDDQFSMAVIDNG
                     CGMSAEMVEKVLDPFVTSRTTRKVGLGLPLLKAAAEACNGSLTIQSTLNQGTKVEVNF
                     QRSHIDRMPLGDLGSTFISLLIGAPEVHWLFRYRVNDQLYELDSQPILETLDGVPLTD
                     PAVLAYLREEIDGGVRTIRPADSF"
     gene            645..1013
                     /locus_tag="PKL11_10025"
     CDS             645..1013
                     /locus_tag="PKL11_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ13977.1"
                     /translation="MPTVKNLEDLKRIREEALEKRKVKTAEGKAQVIVAMGTCGIAAG
                     ARETMKAILAIIEEESLADIVVSQTGCIGLCEREPIVQVVVGDQAKVTYGKVNPEMAR
                     RIVKEHVVGGKIITDHVIPM"
     gene            1044..>1683
                     /locus_tag="PKL11_10030"
     CDS             1044..>1683
                     /locus_tag="PKL11_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ13978.1"
                     /translation="MKYYRTHVLVCMDPQCRRKGAREVVQALQTELAANGLTDEVQVL
                     ETSRIGGCDEGPELMVYPEGVHYANLTPDDMAFIVEEHLLKGRIAKKFLIETTTQADE
                     ELRAPTPKEIRIVLRNCGQIDPQNIEDYIAEEGYMALAKVLNGMSPDDVISEVLKSGL
                     RGRGGAGFPTGRKWALARDVIGEEKYIICNADEGDPGAFMNRRVLEGDPHSVV"
     assembly_gap    1684..1783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1784..2980
                     /locus_tag="PKL11_10035"
     CDS             <1784..2980
                     /locus_tag="PKL11_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNZ13979.1"
                     /translation="PGAFMNRRVLEGDPHSVVEGMIIAAYAIGATRGYIYCRAEYPIA
                     VSTLRIAIDQAREMGFLGENIFGTGWNFDLEVRMGAGAFVCGEETALMSSIEGKRGEP
                     RPRPPFPAQRGLWGKPTNINNVETYANVPQIILRGADWYASMGTEKSKGTKTFALAGD
                     VRRTGLIEVPLGITLREIVYDVGGGIKDDKAFKAIQTGGPMGGCIPVEYLDMPVDYES
                     LAKVGSIMGSGGMIVMDDETCMVDIARFFMEFTQDESCGKCTPCRVGTRRILEILQRI
                     CNGEGKEDDIETLEMLCAEIKADSLCGLGQGAPNPVESTLRFFRDEYEAHIREKRCPA
                     KVCRALIRYEILPDSCTGCTVCARNCPVNAITGERKQPHFIDPDTCIRCGICMQVCNF
                     NAIVIR"
     gene            3011..3490
                     /gene="nuoE"
                     /locus_tag="PKL11_10040"
     CDS             3011..3490
                     /gene="nuoE"
                     /locus_tag="PKL11_10040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNZ13980.1"
                     /translation="MTVETKASPGVLETVKAVVERHGAVKEELIPILNEVNRLLGYLP
                     AEALDEISNLLHVPKSQLFSVATFYSMLTTKPRGRHVVQFCESAPCHVAGGRKVWVTL
                     MDALGIKPGETSADGKWSLVTTSCLGVCGVGPVMLIDDDIYGNVESDQIAGILAKYE"
     gene            3502..>3785
                     /locus_tag="PKL11_10045"
     CDS             3502..>3785
                     /locus_tag="PKL11_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ13981.1"
                     /translation="MKAVRSMVLVSSDPKSLERGAQQVFQRLQEEIKAFGLENEVSLS
                     MVGDVGRHDAVPMVIVYPEAVIYGPVKPEDVHFLVEEHLYKGRVAAPLQAS"
BASE COUNT          859 a          931 c         1104 g          791 t
ORIGIN      
        1 aacaaattcc cgcagttggt ccgctcagtt caggaaccgg aagcgtaatt tgctgtgaga
       61 gaactttcgc tgcacttgct tgatattgcc gagaacagcg tggcagccag ggcgacaacg
      121 atttttgtgg tcgttgaaga gaatctggtt gacgatcaat tcagcatggc tgtgattgat
      181 aacggctgcg gcatgagtgc agagatggtg gagaaagttc ttgacccgtt tgtaacctcc
      241 cgcaccaccc gaaaagtggg gctgggactg ccgctgctga aggcagccgc agaagcctgc
      301 aacgggtcgt tgaccatcca atcgacatta aaccagggca ccaaggtaga agtgaacttt
      361 caacgaagcc acattgaccg gatgccgctg ggtgacctgg gaagcacttt catcagcctg
      421 ctgattggtg cccctgaagt tcactggctg tttcgctacc gcgttaacga ccagttgtac
      481 gaattagaca gccaacccat cctggaaacc ctggatggtg tgccgctcac agatccggcc
      541 gtactggcct atctgcgaga agaaattgat ggcggtgtgc ggaccatccg ccccgctgac
      601 tcgttctaag aaatccaaga cttttgtttc tgaggagcaa aaccatgcct accgtcaaaa
      661 atctcgaaga tttgaagcgc attcgtgaag aagccctgga aaagcgcaag gtcaagaccg
      721 ccgagggcaa agcccaggtg atcgttgcga tgggcacctg cgggattgcc gcaggcgcgc
      781 gcgagacgat gaaggccatc ctggcgatca ttgaagaaga aagcctggcc gacatcgttg
      841 tttcgcagac cggctgcatt gggctgtgcg aacgtgaacc cattgtgcag gttgtggtcg
      901 gcgaccaggc caaagtgacc tatggtaaag tgaaccctga gatggcgcgc cgaattgtca
      961 aagaacatgt ggtgggcggc aagatcatca ccgatcatgt tatccccatg tagagcgtcc
     1021 atatttaaat caggatataa gcaatgaagt attatcgtac acatgtatta gtctgtatgg
     1081 atccccagtg ccgccggaaa ggcgcgcgcg aggtcgtgca ggcgctgcaa actgaactgg
     1141 cggccaatgg gctgactgac gaagtacagg ttctggaaac ctcacgtatt ggcggctgtg
     1201 acgaaggccc tgaattgatg gtgtatccgg aaggcgtcca ctacgctaac ctgacgcccg
     1261 atgatatggc ttttattgtt gaagagcacc tgctgaaggg ccgcattgcc aagaagtttc
     1321 tcatcgagac cacgacacag gccgacgaag aactgcgcgc ccccacgccg aaagaaattc
     1381 gcattgtgct gcgtaactgc ggtcagattg atccgcagaa tattgaagat tacatcgctg
     1441 aagaaggcta tatggcgctg gcaaaggtgc tgaacggcat gagccctgac gatgtgatca
     1501 gcgaagtgct gaaatctgga ctgcgcggcc gcggcggtgc gggcttccca accgggcgca
     1561 aatgggcgct ggcgcgggac gtgattggcg aggaaaaata tatcatctgc aatgcagatg
     1621 aaggcgaccc gggtgcgttc atgaaccggc gtgtgctgga aggcgaccct cactcagtgg
     1681 ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgggtg cgttcatgaa
     1801 ccggcgtgtg ctggaaggcg accctcactc agtggttgag ggcatgatta ttgcggcata
     1861 tgccattggc gcaacccgcg gttacattta ctgccgcgcc gagtacccca tcgccgtcag
     1921 cacgctgcga attgcaattg accaggcccg cgagatgggt ttcctgggag aaaacatctt
     1981 cggcactggc tggaactttg atctggaagt tcgcatgggc gctggcgcct ttgtgtgcgg
     2041 cgaggaaaca gcgctgatgt cttcaattga aggtaagcgc ggcgagcctc gcccgcgtcc
     2101 gcccttcccg gcgcagcgcg gtctgtgggg caaacccacc aatatcaaca acgttgagac
     2161 gtatgccaat gtgccgcaga tcattctgcg cggtgcagac tggtatgcct cgatgggtac
     2221 cgagaaaagc aaaggcacca aaacctttgc gctggccggc gacgttcgcc gcacgggcct
     2281 gattgaggtt ccactgggca ttacgttgcg cgaaattgtt tacgatgtgg gcggcggcat
     2341 taaggatgac aaggccttca aagccatcca gaccggcggc ccaatgggcg gctgtattcc
     2401 ggtggaatat ctggatatgc cggtggatta cgaatcactg gctaaggtgg gttccatcat
     2461 gggctctggc ggtatgatcg tcatggacga tgagacctgt atggtggata ttgcccgctt
     2521 ctttatggaa tttacccagg acgagtcttg cggtaaatgc acgccttgcc gggtgggtac
     2581 ccgccgtatt cttgagatat tgcagcggat ttgcaacggc gaaggcaaag aagacgacat
     2641 tgaaactttg gaaatgctgt gcgctgaaat caaggctgac agcctgtgcg gtctgggaca
     2701 gggcgcaccc aatccggttg aaagcacgct gcggttcttc cgcgatgagt acgaagctca
     2761 tattcgcgag aagcgctgcc ccgccaaagt ttgccgcgcg ctgatccggt atgaaatttt
     2821 gcccgatagc tgcaccggct gtacggtttg cgcccgcaac tgtcccgtga acgccattac
     2881 tggcgaacgg aaacaaccgc acttcattga ccccgatacc tgcatccgtt gtggcatttg
     2941 tatgcaagtc tgcaacttca atgccattgt gattcgctaa ctgcaatcca atctgaccgg
     3001 gagggaccca atgaccgtag aaacaaaagc aagcccaggc gttcttgaaa cagtgaaagc
     3061 tgtggttgag cgtcacggcg ccgtcaaaga ggaattgatc ccgattctga acgaagtgaa
     3121 ccgcttgctg ggttacctgc cagcggaagc actggacgag atcagcaacc tgctgcacgt
     3181 acccaagagc cagctcttca gcgtggcgac attttattcg atgttgacca ccaagccgcg
     3241 ggggcggcac gtggtccagt tctgcgaaag cgccccctgt catgtggcgg gcgggcgaaa
     3301 agtgtgggtg actttgatgg acgcactggg catcaagccc ggtgaaacca gcgccgatgg
     3361 caaatggtca ttggttacga ccagctgtct gggtgtctgc ggtgttggtc cggtcatgct
     3421 gattgatgat gatatctacg gtaacgtgga atccgatcag attgctggca ttctggctaa
     3481 gtacgagtaa aggagcaggc gatgaaagct gtacgctcaa tggtgttagt ttccagtgac
     3541 ccaaaaagcc tggaacgcgg cgctcagcaa gttttccagc gcctgcagga agagattaag
     3601 gcttttgggc tcgaaaacga agtatcactt tcgatggttg gcgatgtagg acggcacgat
     3661 gcggttccga tggtcattgt ctacccggaa gcggtcattt acgggcccgt gaaaccagaa
     3721 gatgttcatt ttctggttga agagcacctc tataaaggcc gggtggcggc gccgctgcaa
     3781 gcttc
//
LOCUS       DAOPVS010000174         3751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_24829_length_3751_cov_58.979978, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000174 DAOPVS010000000
VERSION     DAOPVS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24829_length_3751_cov_58.979978"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..870)
                     /locus_tag="PKL11_10050"
     CDS             complement(28..870)
                     /locus_tag="PKL11_10050"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HNZ13982.1"
                     /translation="MFEKISKSWDLVKASAKVLSQDKELVIFPIISSIAVVLVSIGFF
                     VPTIFASMADAALGDTQIIYYLALFLFYVVQYFVIFFFNTALVGAAMIRLRGGDPTVG
                     DGFRIAFSRFMPLLGYSLIAATVGMILRIISDKSKGLGRAIVSIIGFAWNVATFLVVP
                     ILAVENVGPIEAIKRSVALLKKTWGEQIVGNFGLGAFFGLIIFGIMLLGFGGSVALFL
                     MLDSYIPGLVLIALTIMVLVLVGLAQSTLNGIYTAALYQYATEGQTTAGFEPEVVHDA
                     FLPK"
     gene            complement(903..1811)
                     /locus_tag="PKL11_10055"
     CDS             complement(903..1811)
                     /locus_tag="PKL11_10055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ13983.1"
                     /translation="MKTTTSTSIPELKILTAEAWNDYELLDSGNGARLERFGGITLVR
                     PEAEAIWQPALHEKAWKAAQAVFRPGQEENGGHWETSKPLPERWQIQRGGLKFWLQPG
                     GSKQVGIFPEQAPQWDWIEAQVQAARRPIRVLNLFGYTGIASLAAARAGAQVTHLDAS
                     RKVMTWAHENQQLSGLPDTSIRWMLEDALKFVQREARRGSRYDGLILDPPKFGRGPQG
                     EVWEFYKLLPSLLAACQQVISAEPRFVVLTAYAVKASALTLQAAMEDMMSDKHGSVEA
                     GETALREQARGRLLSLALFARWAATD"
     gene            1938..3110
                     /locus_tag="PKL11_10060"
     CDS             1938..3110
                     /locus_tag="PKL11_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNZ13984.1"
                     /translation="MPHPFDEIPDRRATESIKWRAYPADVLPMWVADMDFRSPEPVIH
                     ALQNRIAQGIYGYPGQVDGLIEAILAWLETRHGWKIDPAAIMLVPGIVTGLNWFCNAL
                     GQPGESVLVQSPVYPPFLSAPGNAGLHLQQMPLKVWGGRYSPDAAAFDSACSAAAADG
                     HPASLFILCNPHNPVGRVFTRPELESIAAACLRHNLTICSDEIHADLVYSESRHIPIA
                     SLDPEIAARTVTFMAPSKTFNIAGLECSFAIVPNPDLRERLQQGGRGLVGWVNLLGQV
                     AAQAAYAESAPWLVDTLDYLQANRDYLYNYIMVEKRLPGVTMTLPEATYLAWLDCRAT
                     GIDNPYAFFLQKARVALNFGGEFGLGGPGFVRLNFGCPRPMLTKALDRMRRALEQR"
     gene            3230..3736
                     /locus_tag="PKL11_10065"
     CDS             3230..3736
                     /locus_tag="PKL11_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNZ13985.1"
                     /translation="MADSARPADAIVLLSGGGPERITEAAHLYRDFKVPRVITTETGV
                     WNDAFEQPATLFIHRELIRQGIPADNIITTSGQANSTFNEARAVREVLESRNLTSCIV
                     VTDPFHTFRTRLIFRQVFRGSGISVWVRPAREHWYRSNTWFTTRAGWRITLLEYLKLL
                     AYLLGIRR"
BASE COUNT          795 a         1234 c          915 g          807 t
ORIGIN      
        1 attatctgag tctcaactca gccgttttta cttcggcaga aaggcatcat gtaccacttc
       61 cggctcaaag ccagcagtgg tctgaccttc cgttgcatat tgatacagcg ccgcggtgta
      121 gatgccgttc agcgtggact gtgccagccc gaccagaacc agtaccatga tcgtcaatgc
      181 aatcagaacc agcccgggaa tgtagctatc cagcattaag aacagcgcca ccgaaccgcc
      241 aaaacccagc agcataatcc cgaagataat caagccaaaa aaggcgccca gtccaaaatt
      301 gcccacaatc tgctcgcccc aggtcttctt gagcagtgcc acgctgcgct tgatggcttc
      361 gatgggccca acattttcga ctgccagaat gggcaccacc aggaacgtcg ccacgttcca
      421 tgcaaaccca atgatggaaa caatggctcg ccccaggccc ttgcttttat ccgaaatgat
      481 ccgcaggatc atgcctaccg tcgcagcaat caatgaataa cctaacagcg gcatgaaccg
      541 gctgaacgca atcctgaagc cgtcacccac ggtcgggtca ccgccgcgca gccggatcat
      601 cgcggcacca accagcgccg tgttgaaaaa gaagatcaca aaatactgca ccacataaaa
      661 cagaaacaac gccagatagt aaataatctg agtatcgccc agcgccgcat ctgccatact
      721 ggcaaagatg gtgggcacaa aaaagccaat gctcaccaga accaccgcaa tcgatgaaat
      781 gattggaaag atcaccagtt ccttgtcttg cgacaggact ttggcgcttg ccttgaccag
      841 gtcccagctt ttagaaattt tttcaaacat tttgtcctcc ggaaaatata cggaaaaata
      901 gatcaatcag ttgcagccca acgggcaaat aaagccagcg aaagcaagcg cccgcgcgcc
      961 tgttcgcgca gcgctgtctc gccagcttct acagacccat gcttgtcgct catcatatct
     1021 tccattgccg cctgcagcgt cagcgccgaa gctttaaccg cataagccgt cagtaccaca
     1081 aagcgcggtt ctgcgctgat cacctgctgg caggctgcca gcagcgaagg cagcagctta
     1141 taaaattccc atacctcacc ctgcgggccg cgtccaaact tgggcgggtc caaaatcagc
     1201 ccatcatacc gtgacccacg ccgcgcctct cgctgcacaa atttcagcgc gtcttccagc
     1261 atccagcgga tcgatgtgtc tggcagcccc gagagctgct gattctcatg tgcccaggtc
     1321 atcaccttgc gcgaggcatc caggtgtgtc acctgtgccc cggcgcgtgc cgctgccagg
     1381 ctggcaatcc ctgtatatcc aaacagattc aaaacccgaa tgggccgccg ggctgcctgt
     1441 acctgcgcct caatccaatc ccactgcggc gcctgttccg ggaaaatgcc cacttgttta
     1501 gagccccccg gctgcagcca gaatttcagc ccgcctcgtt gaatctgcca ccgctcgggc
     1561 agcggcttgc tggtctccca gtgcccgccg ttctcttcct gcccgggccg gaacacagcc
     1621 tgagctgctt tccaggcctt ctcgtggagc gccggctgcc agatggcctc agcctcaggc
     1681 cgcaccagcg tgatgccccc aaaccgttcc aggcgggcac cgttgccgct gtccagcagc
     1741 tcataatcgt tccaggcctc ggccgtaaga atctttaatt caggtatgct tgtagatgtt
     1801 gttgttttca tagtaactgc gatttattgt agcagattct tgcaaaaaaa taaccttggc
     1861 gccctcagaa cttcaaactg aaacttgaaa tgtaaaaggc tgttataatc cccaaaacaa
     1921 cacctggagg aagtcctatg ccgcacccat ttgatgaaat tcctgaccgc cgcgcaaccg
     1981 aaagcattaa atggcgagct tatcccgccg atgtcctgcc catgtgggtg gccgacatgg
     2041 atttccgctc tcctgagccg gtgattcacg ccctgcagaa tcgcatcgcc cagggcatct
     2101 atggttaccc cggtcaggtg gatggtttga ttgaggctat tctagcctgg ttggaaaccc
     2161 gccatggctg gaagattgat cctgcagcca tcatgctggt gcctggcatt gtcactggcc
     2221 tcaactggtt ctgcaatgcc ctcggccagc ccggtgagtc ggttctggtg cagtccccgg
     2281 tttacccgcc tttcctcagt gcccctggta atgccggcct gcacttgcag caaatgcccc
     2341 tcaaggtttg gggcggacgc tattcaccag atgccgcggc ctttgattca gcctgcagcg
     2401 ccgctgccgc cgatggacac cctgccagcc tgttcatcct gtgcaatccc cacaacccgg
     2461 tgggccgcgt ctttacccgc cccgaactgg aatcgattgc cgcagcctgt ttgcgccata
     2521 atctgaccat ttgctccgac gaaattcacg ccgacctggt ctattccgaa agccgtcaca
     2581 ttcccatcgc ctcgctggac cccgaaattg ccgcccgaac tgtgacattc atggccccca
     2641 gcaaaacctt caacattgcc gggttggaat gttcctttgc cattgtcccc aatcccgacc
     2701 tgcgcgaacg tctgcagcag ggtggtcggg gcctggtggg ctgggtcaat ttgctgggac
     2761 aggttgctgc ccaggctgcc tacgccgaga gcgctccctg gctggtagac acattggatt
     2821 acctgcaagc caatcgtgat tatctttaca attacattat ggtcgaaaaa cgcctgcccg
     2881 gcgtgaccat gaccttgccc gaggccacct atctggcctg gttggactgc cgcgccaccg
     2941 gcattgataa cccctatgcc tttttcctcc aaaaagcccg cgtggccctc aacttcggcg
     3001 gcgagttcgg cctcggcggt cccggctttg tccgcctcaa ctttggctgc ccgcgcccca
     3061 tgctgacgaa agctctggat cggatgcgcc gggcactgga gcagcgctga tactgagatc
     3121 acgcgcccgc cgtaaattcc tcccgattct cctggcgctg ccattcattc tggcattact
     3181 ggctgttggc gtctatctgg cgctgtgggg tgccggcgca ttacttgttg tggccgactc
     3241 cgcccgcccc gccgatgcca tcgtcttgtt gagcggcggt ggccctgagc ggattaccga
     3301 ggccgcgcac ctttaccgtg acttcaaagt tccccgcgtc atcaccaccg aaaccggcgt
     3361 ctggaacgat gcttttgagc agccagctac cctctttatc catcgcgaac tgatccgcca
     3421 gggcatcccc gccgacaaca tcattacgac atccggtcag gccaatagca ccttcaacga
     3481 agcccgtgcc gttcgagaag tcctggaatc ccgcaatctg acctcctgca ttgtcgtcac
     3541 tgaccccttc cataccttcc gcacccgcct catcttccgc caggtctttc gcggcagcgg
     3601 catctcagtc tgggtccgtc cggcgcgtga gcattggtac cgctccaaca cctggttcac
     3661 cacccgcgcc ggctggcgaa tcaccctgtt agaatatcta aaactcctcg cctatctcct
     3721 cggcatccgc cgctaacccc cgtcaatccc t
//
LOCUS       DAOPVS010000175         3728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_25044_length_3728_cov_29.013613, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000175 DAOPVS010000000
VERSION     DAOPVS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25044_length_3728_cov_29.013613"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..480
                     /locus_tag="PKL11_10070"
     CDS             46..480
                     /locus_tag="PKL11_10070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNZ13986.1"
                     /translation="MPIKNVSIEAVQVEGFKIETRSRQHIALVDQPVAGGGTDSGPTP
                     LEYLFVSLAGCIVTIGHIVAKQRQLPVRSISARVEGELDTDVLMGKRSDVRAGFSDIR
                     IHVKIDADMTQAEKEAFLKEVDARCPISDNIHGTTPLNFVVE"
     gene            488..1531
                     /locus_tag="PKL11_10075"
     CDS             488..1531
                     /locus_tag="PKL11_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ13987.1"
                     /translation="MSAVDPSNVSDQPPTEVQVGRRIRELRTRSGLSLRALADRSGLN
                     INTLSLVENGKSSPSVSTLQQLAQALDVHISTFFETEPTLKHVVFTPRDQRPHAVVGS
                     TQMQNLGKDLYGSVVQPFVVTLKPGMGSGERLIVHTGHEFVYCLEGIARYQIDGAEYV
                     LRAGDSLVFEAHLPHCWENHGDSTTQILLVLLPSDERDQPAGRHLSLEYNQKDITMKI
                     AVITDDGKLISQHFGRAPYYLVLTIEDGKVTQRELRNKLGHNQFSAHEHTEEPHAAGH
                     GLDAASHNKHASMADAISDCEALLCGGMGMGAYESMRRLNIKPIVTDIRDIDAALQAF
                     IEGKLVDHTELLH"
     gene            1567..2418
                     /locus_tag="PKL11_10080"
     CDS             1567..2418
                     /locus_tag="PKL11_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNZ13988.1"
                     /translation="MTEFPPLVNILLLSLMASLGTGIGGLIATIRRPGKRSYGLLMGI
                     TAGVMICLSFLELVNEAWEMAGPWTATIGFGAGATFMFLLDHFAPHIRFGEREIRGKG
                     DTNAVIPVDDEHCYRRRRFGLRHRQGPAVVVDQKLINTGLLMAIGITLHNLPEGIAVG
                     AGYLHNPNFGLFIAVAILLHNIPEGIATALPLCKGGVCKADALRVAFLSGLAEPVGAL
                     LASLVLVSFKGFLPGALAFAGGVMVFITLDELIPTAREYGHEHYTAIGIILGSMFVFI
                     LSGIFGV"
     gene            2423..2869
                     /gene="tsaA"
                     /locus_tag="PKL11_10085"
     CDS             2423..2869
                     /gene="tsaA"
                     /locus_tag="PKL11_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNZ13989.1"
                     /translation="MPITINPIGIIRTSLIEKAQAPIQSARSQVSGIAEVYDAFVAGL
                     EGIEEFSHIYLVYYFHQSRPEFALRVQPFLDNKQHGVFATRYPVRPNAIGFSVVQLTH
                     RQDNLLYFNGADMLNETPLLDIKPYIPEFDIFSVDKIGWYQNRSSQ"
     gene            2913..3620
                     /gene="prxU"
                     /locus_tag="PKL11_10090"
     CDS             2913..3620
                     /gene="prxU"
                     /locus_tag="PKL11_10090"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_081651649.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:3159..3161,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HNZ13990.1"
                     /translation="MSDKPEGCVKPTQGPILGNVVNLETGPEIQKEVSMVQARVGKPA
                     PDFELSAFIGGGFKNIKLSDYKGKWIVICFYPGDFTFVUPTELSAVAVKYKELQELGV
                     TILAISTDSRFSHKIWQEEELSKMVEGGVPFPLLSDAGGRVGSIYGVYDEDAGVDIRG
                     RFLIDPDFNIRAMEVMTPEVGRNVMELIRQVKAFQHVRATGEVTPAGWQPGKATLKPG
                     PDLAGKVWKVWKPDQAF"
BASE COUNT          880 a          904 c         1036 g          908 t
ORIGIN      
        1 attatgtact ataattttag tatcttattg ttgcggagga aaagaatgcc aatcaaaaat
       61 gtaagtatcg aagctgtaca ggttgaaggt ttcaaaattg agacccggtc aagacaacac
      121 attgcacttg tagatcaacc tgttgcgggc ggcggcaccg attcgggacc gacaccgttg
      181 gaatatctgt ttgtttcgct ggctggttgt attgtgacca ttggacatat tgttgccaaa
      241 cagcgccagc tgcccgtccg cagcatttca gcgcgggttg aaggcgagct ggataccgat
      301 gtgctgatgg gaaaacgatc tgatgtgcgc gccgggttct cggatatccg gatccacgtc
      361 aaaattgacg cggatatgac ccaggccgag aaagaagcct tcttgaagga ggttgatgcg
      421 cgctgcccga tctcggacaa tattcacggc accaccccct tgaattttgt ggtggagtaa
      481 agagcacatg tctgctgtcg atccgtccaa tgtgtccgat caaccgccaa ccgaggtaca
      541 ggttggtcgc cggattcgag aactgcgcac acgcagcggc ttatccttgc gcgcattggc
      601 ggatcgcagc ggattaaata tcaataccct cagtctggtg gaaaatggga agtcttcgcc
      661 ttcggtaagt acgctgcaac agcttgcaca ggcgctggat gttcatattt caacgttttt
      721 tgagactgaa cccaccttga agcatgtggt atttaccccc cgcgaccagc ggccccatgc
      781 agtagtgggc agcacccaaa tgcaaaacct gggaaaagac ctctacggca gcgtcgtcca
      841 gccctttgtg gtcacattaa agccggggat gggcagcggt gaacggttga tcgtccacac
      901 cgggcatgaa tttgtgtatt gcctggaggg cattgcccgt taccagattg atggagcaga
      961 atatgttttg agggcaggcg acagcctggt gtttgaggcg cacctgccgc actgctggga
     1021 aaaccatggc gatagcacaa cccagatttt gctggtattg ctgccttccg acgaacggga
     1081 tcagcccgct ggaaggcacc tctcacttga atacaatcaa aaggatatca caatgaaaat
     1141 tgcagttatt accgatgacg ggaaattgat cagccagcat tttgggcggg cgccttacta
     1201 tctggtattg acgattgagg acggcaaggt aacgcagcgc gaattacgca acaaactggg
     1261 gcacaaccag ttcagtgccc acgagcatac tgaagaacct catgcggctg gacacggcct
     1321 ggatgcagct tcgcacaata aacacgccag catggcagat gccatatccg actgtgaagc
     1381 attgttgtgc ggcggcatgg gcatgggtgc ctatgagagc atgcggcggc tcaacattaa
     1441 gccgatcgtc acagacatcc gagatattga tgcggctctg caggctttta ttgaaggaaa
     1501 attggtcgat catacagagc tgcttcacta gaagccgatc aaagtggatc actggatacc
     1561 cgattgatga ctgaatttcc tccgctggtc aatatcttgc tgctcagctt aatggccagc
     1621 cttgggacgg ggattggcgg actgatcgcc accatccgtc gtccgggcaa acggtcttac
     1681 gggctgttga tgggaatcac agccggagtg atgatttgtc tgtcgtttct ggaactggtt
     1741 aacgaagcct gggaaatggc ggggccatgg actgcaacga ttggctttgg cgccggggcc
     1801 acgttcatgt tcctgctgga ccattttgcg ccgcatattc ggtttggaga aagagagatt
     1861 cgcggaaaag gggacaccaa cgcggtaata cccgttgatg atgagcactg ctaccgaagg
     1921 cggcgattcg ggctgcgaca tcggcaaggc ccggcggtgg tggtagatca aaagctgatc
     1981 aataccgggt tattgatggc cattggcatc acgttgcaca atctaccaga aggaatagct
     2041 gtgggcgcgg ggtatttgca taaccccaat ttcggcctgt ttatcgccgt agccattttg
     2101 ctgcacaata tccccgaagg cattgcaacc gccctgccgc tttgcaaggg aggggtctgt
     2161 aaagcggatg ctttgcgggt ggcgttctta tcgggcctgg cggagccggt gggcgccttg
     2221 ctggcaagcc tggtgctggt ctcttttaaa ggatttttgc ccggagcgct ggcttttgcc
     2281 ggcggcgtga tggtgttcat cacgctggat gagttgatcc ctacggcccg ggaatatgga
     2341 cacgaacatt acactgccat tggaattatc ctggggtcga tgtttgtgtt tattctgtca
     2401 ggtatttttg gtgtgtagaa gcatgcctat tacgatcaat ccaattggca ttatccgcac
     2461 ctcgctgatt gagaaggcac aggcgccaat ccaatctgcc cggtctcaag tttctggaat
     2521 tgctgaagtt tatgatgcgt ttgtggctgg acttgaggga attgaagaat tctcccatat
     2581 atatcttgtc tattatttcc accaatccag gccggaattt gcgttgaggg ttcaaccgtt
     2641 tctggacaac aaacagcatg gcgtttttgc cacccgatat cctgtgcgcc caaacgccat
     2701 tgggttttct gttgtgcagt taacccatcg tcaggataat ttgctatact ttaatggtgc
     2761 tgatatgctg aatgaaaccc cattgctgga tatcaaacca tatatcccag agtttgatat
     2821 tttcagcgtg gataagattg gctggtacca gaatcgttcc agtcaataag cctgttttga
     2881 gattttgcgc cgggtcgtca taaggaggtt caatgtcaga caaaccagaa ggctgcgtta
     2941 agccaaccca ggggccaatc ttgggaaatg tggttaacct ggagactggc ccagagatcc
     3001 aaaaggaggt gtcaatggtt caagcacgcg taggcaagcc cgcccccgac tttgaattga
     3061 gcgcatttat cggcggcgga tttaagaata tcaagctgtc agactataag ggaaagtgga
     3121 ttgtcatctg cttctacccg ggtgatttca cctttgtctg accgaccgaa ttgtcggcag
     3181 ttgccgtcaa atataaagaa ctccaggaac tgggcgtcac cattctggca atcagcacag
     3241 acagccggtt ctcacataaa atctggcagg aagaagaact ttcgaaaatg gttgagggcg
     3301 gcgttccctt cccgctgctc tcagatgccg gtgggcgggt aggcagcatc tatggtgttt
     3361 atgacgaaga tgccggtgtg gatatccgcg gtcggttctt aattgacccc gatttcaaca
     3421 ttcgcgccat ggaagtaatg acccccgaag tgggtcgaaa tgtcatggaa cttatccggc
     3481 aggtcaaggc cttccaacat gtgcgcgcca ccggtgaggt tacgcccgcg ggctggcagc
     3541 ccggcaaagc caccctcaag cccggccccg atctggctgg caaagtgtgg aaggtctgga
     3601 agcccgacca ggctttttag ccccgctcgt atttagtaat ccggcgcatt ttcatctgga
     3661 ggttgaggca gtgactgctt caacctccag aatgttttca agaaatactg acacacatca
     3721 accgccca
//
LOCUS       DAOPVS010000176         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_25048_length_3727_cov_43.409586, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000176 DAOPVS010000000
VERSION     DAOPVS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25048_length_3727_cov_43.409586"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKL11_10095"
     CDS             <1..266
                     /locus_tag="PKL11_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ13991.1"
                     /translation="HTICWMAPFMILGRKLRNTLAWPALRLKADPARCTDCLTCNANC
                     PMSLDVNGLVKSGHMEHTECILCGSCVDGCPSKAIVYSFSKGK"
     gene            331..2223
                     /locus_tag="PKL11_10100"
     CDS             331..2223
                     /locus_tag="PKL11_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNZ13992.1"
                     /translation="MFQIRNNHFIDGSGRVALLRGVNLSGATKLPCIPDGATWNPAGF
                     QDAVNVSFVGRPFPLAEADEHFSRLRAWGLTFLRFLVTWEAIEHAGPGLYDEAYLDYL
                     YAVVKKAADYDICLFIDPHQDCWSRFTGGDGAPGWTFDLLGLDRTKFHAAGAAFNHQD
                     CGDPLPKMIWTSNYGKYAAATLFTLFFGGNTFAPQTTIQGVPIQEFLQGHYIAAIQQV
                     AERLREFPHVLGYDTLNEPSQGFIGFPDANQIPFEFASYGALPTIYQSLLLAAGYPQT
                     VKYKPNKLPLPSGQTVVVNSAGLRLYRDGTTPIWQQNGVWDVDAAGSPHLLQPDYFGR
                     VAGQPVNFDQQFFAPFVRRYTAAIRSIHPNALIFVSPPPAEMRIGAEGFTLSDETGLV
                     FAPHWYDGITLSFQKYLPWLGVDTAHQPVRLALGRKNRRRFIARQLGTHLERARRDLG
                     EAPVVIGETGIPFNLEGGRAFRTGDFSAHVQALDDTIQGLEANFLNFTLWNYTPDNTN
                     ARGDLWNNEDLSIFSRDQQTGSGDINDGGRALPAAIRPYAARVPGEPLSTRFDLSTCE
                     FQFTFRLNPAVAAPLEVFLPRYHYPNGAAIQVTRGRVQVDLPNQRLEYWPDAAETIHT
                     LTLRAA"
     gene            complement(2288..2503)
                     /locus_tag="PKL11_10105"
     CDS             complement(2288..2503)
                     /locus_tag="PKL11_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13993.1"
                     /translation="MSPETSRVLLGEDLIGNVSAYTALPAVICPSCSAKLHRIFNTTT
                     EWRYVCQSEHLLIDPTGEKAPVKISIE"
     gene            2662..3219
                     /locus_tag="PKL11_10110"
     CDS             2662..3219
                     /locus_tag="PKL11_10110"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNZ13994.1"
                     /translation="MPGKTSALTRKAILEAANRIVLEQGVDHLTLEAAAAAAGISKGG
                     LLYHFPSKEALIVGMIRAYLERFTADYNTSAEKEPDQTPGRWTRAYLETTFADNERIP
                     RLSSGLLAAIATDPSLLAPLQQTFADWVAQLSLDGIDPVTATIIRLVADGVWLVELFG
                     LTPPDAAMKARVMEALLEMSRARPA"
     gene            3281..>3727
                     /locus_tag="PKL11_10115"
     CDS             3281..>3727
                     /locus_tag="PKL11_10115"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ13995.1"
                     /translation="MNEIESLEQAIQALETQRALLGDAVVEAGLAPMRERLQVLRRQG
                     VEQQRKQVTILFADASGFTAASALQDPEEVTETMNALWQCVDGVILEYGGTIDKHIGD
                     AVMAVWGMTTTSEDDAERAVRAALSMQQAVKEANLQLGGSPLQIRIG"
BASE COUNT          710 a         1232 c         1009 g          776 t
ORIGIN      
        1 gtcacaccat ctgctggatg gcgcccttca tgatcctggg ccgcaagctg cgcaataccc
       61 tggcctggcc cgccctgcgc ctcaaagcag atccggcccg ctgcacggac tgtctgacct
      121 gcaacgccaa ctgccccatg agcctggacg tgaacggtct ggtcaaatcg ggccatatgg
      181 agcataccga gtgcattctc tgcggcagct gcgtggatgg ctgccccagc aaggccatcg
      241 tttattcttt cagcaaaggc aaataaccct gctgccgggc aatcaccgtt cattgtgatt
      301 gccccaacat caaaattgag agaggttgcc atgttccaga ttcgcaataa tcacttcatc
      361 gacggctctg gccgcgtggc gctgctgcgc ggcgtcaatc tgagcggcgc caccaagctg
      421 ccctgcattc ccgatggcgc cacctggaac ccggctggct ttcaggacgc cgttaatgtc
      481 agctttgtcg ggcgaccctt ccccctggct gaagccgatg agcatttcag ccgcctgcgt
      541 gcctgggggc tcaccttcct gcgcttcctg gtcacctggg aggccattga acatgccggc
      601 cccggcctct atgacgaagc ctacctggat tacctctacg cggtggtcaa aaaagctgcc
      661 gattatgata tctgcctgtt cattgacccc catcaggact gctggagccg ctttaccggc
      721 ggcgatggcg cccccggctg gacctttgac ctgctgggcc tggaccgcac caaattccac
      781 gctgccgggg ccgccttcaa tcatcaagac tgcggcgacc ccttgcctaa aatgatctgg
      841 acctccaact atggcaaata cgccgccgcc acgctcttca cgttgttctt tggcggtaac
      901 actttcgctc cccagaccac catacagggt gtccccatcc aggaatttct ccagggacat
      961 tacatcgccg ccatccagca ggtggccgag cgcctgcgtg aatttccgca tgtgctgggc
     1021 tacgataccc tcaatgaacc ctcccagggc ttcattggct tcccagatgc caatcaaatc
     1081 ccctttgagt ttgcttctta cggcgccctg cccacgatct atcagagcct gctgctggct
     1141 gccgggtatc cgcagactgt caaatacaag cccaacaagc tgcccctgcc ttccggtcag
     1201 acggttgtcg tcaattcggc ggggctgcgc ctctatcggg acggcaccac ccccatctgg
     1261 cagcagaacg gtgtctggga tgtcgatgca gccggcagcc ctcacctctt gcagccggat
     1321 tattttgggc gagtggctgg gcagccggtg aactttgatc aacagttctt tgcgccattt
     1381 gtgcgccgct acaccgccgc catccgttcc atccacccga acgctttgat cttcgtctct
     1441 ccaccgccgg ccgaaatgcg tataggcgcc gagggcttta ccctgtcaga cgagacgggc
     1501 cttgtctttg caccgcactg gtatgatggc atcacgctgt ccttccagaa atacctgccc
     1561 tggctcggcg tggataccgc ccatcaaccg gtgcgcctgg cgctgggacg caagaaccgc
     1621 cgccgtttca tcgcccggca gttgggcacc cacctggagc gcgcccgccg tgacctgggc
     1681 gaggccccgg tagtcatcgg tgaaactggc atccccttca acctggaggg cggccgcgcc
     1741 ttccgcaccg gcgatttttc cgcccacgtt caggccctgg acgataccat tcagggcctt
     1801 gaggccaatt tcctcaactt caccctctgg aattacaccc ccgataacac caacgcccgc
     1861 ggcgatctat ggaacaatga ggacctgtcc atcttcagcc gtgaccagca aaccggcagc
     1921 ggcgacatta atgacggcgg gcgggcgttg cctgccgcca tccgtcccta cgcagcccgc
     1981 gtccccggcg agcccctgtc cactcgcttt gacctgtcca cctgcgagtt ccaattcacc
     2041 ttccggctca acccggcggt cgcggcgccg ctggaggttt tcctgccccg ctaccattac
     2101 cccaacggcg ctgccattca ggtgacccgc ggccgcgtgc aggtggatct gcccaaccag
     2161 cgcctggaat actggcccga tgccgcagaa acaatccata ccctcacact tcgtgccgcc
     2221 tgatgtcctg atcctgattt cccccgtgga ttaacagaac agccccgcgt ttcggggctg
     2281 tttgtttcta ctcaatgctg atttttacgg gagccttctc accggtgggg tcaatcagca
     2341 gatgttcaga ctggcagaca tagcgccact cggtggtggt gttgaaaatc cggtgcagct
     2401 tggcgctgca gctggggcag atgactgccg gcagggcggt gtaggcgctg acattgccaa
     2461 tcagatcctc acctaataag acacgactgg tttcgggtga cacaattttc ctcctgtgag
     2521 acatattgga ccggcatccc aaagggctcg ccggccattt tgcggttaaa ataaccctgt
     2581 gattaatctg ctacagtatt aatactatac cgtccagacg gtataattat agcacaggtt
     2641 tgagaataaa ggaataccac catgcccggt aaaaccagcg ccctcacccg taaagccatc
     2701 ctggaagccg ccaaccgcat cgtccttgaa cagggcgtcg atcacctcac gttggaggcg
     2761 gccgcggctg cggcgggcat cagcaaaggt gggctgctgt accacttccc cagtaaagaa
     2821 gccctgattg tcggcatgat ccgggcttac ctggaacgct ttactgccga ttacaatacc
     2881 tcggctgaaa aagaacccga tcaaacccct gggcgctgga cccgtgccta ccttgaaacc
     2941 acctttgccg ataacgagcg catcccccgt ctgagctcgg gccttctggc tgccatcgcc
     3001 acggatccct ccctgctggc gcccttgcag caaacctttg ccgattgggt tgcccaactc
     3061 tctctggatg gtattgaccc cgtgaccgca accattatcc gcctggtggc cgatggcgtc
     3121 tggctggtgg aactgtttgg cctcaccccg cccgatgccg ccatgaaagc ccgggtgatg
     3181 gaagccctgc tcgaaatgtc tcgcgcccgc cccgcctagg cccggcggtc ttcatcctcg
     3241 ggtataatag atttcatccc cttctgctca ggaataatcc atgaacgaaa tcgaaagcct
     3301 cgaacaggcc attcaggctc tggaaactca gcgggccctg ctgggtgatg cggttgtgga
     3361 agccgggcta gctcccatgc gcgagcgcct gcaggtgctc cggcgccagg gcgtcgagca
     3421 gcagcgcaag caggtcacca tcctgtttgc tgatgcctcg ggcttcactg ccgcctccgc
     3481 cttgcaggac cccgaagaag tcaccgagac gatgaatgcc ctctggcagt gcgtggatgg
     3541 tgtgattctg gagtacggcg ggacgattga caagcacatc ggcgatgccg tgatggctgt
     3601 ctggggaatg accaccacca gcgaagatga cgccgagcgc gccgtgcgtg cggctctgtc
     3661 tatgcagcag gcggtcaaag aagccaacct gcagttgggc ggcagcccgc tgcaaatccg
     3721 cattggc
//
LOCUS       DAOPVS010000177         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_25136_length_3719_cov_33.421943, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000177 DAOPVS010000000
VERSION     DAOPVS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25136_length_3719_cov_33.421943"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..346)
                     /locus_tag="PKL11_10120"
     CDS             complement(104..346)
                     /locus_tag="PKL11_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNZ13996.1"
                     /translation="MINIKVLGPGCANCKRVEAAARKVVAELQLEAEIEKVTDMAEIM
                     KWPILSTPGLVINGQVVASGRIPTEAEIAAWLKQAA"
     gene            complement(367..741)
                     /locus_tag="PKL11_10125"
     CDS             complement(367..741)
                     /locus_tag="PKL11_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ13997.1"
                     /translation="MELNVVTHTQPLERQAQIFKALTHPARLAILEILRDGEHCVCHM
                     EAHLGYRQAYISQQLAVLREAGLIQDRRDGWNIFYRVSRPEVYQLIDVVAEMTGERPA
                     PAKTITGTDCPCPQCAAKRKHP"
     gene            1031..1432
                     /locus_tag="PKL11_10130"
     CDS             1031..1432
                     /locus_tag="PKL11_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ13998.1"
                     /translation="MEGKNASSQIGVQSGSVTRNHILWLLYSAAFGLALVIQILSSAL
                     TWYRTITYDYFCGNPLMGDWALWFVISLILGALVIPGGIWSIRHRKQRMGIIYRVGSL
                     LYLLSVVVMLTAGIFWGTVPDFLNFIGYAFG"
     gene            1582..2424
                     /locus_tag="PKL11_10135"
     CDS             1582..2424
                     /locus_tag="PKL11_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNZ13999.1"
                     /translation="MFQIGEFSRIACVTIDTLRHYDALGLLKPVKVDPITGYRYYSAK
                     QLQPLNRIIALKEIGLSLKEIALILQDNLSIDELRGILKVQLALAESVIEATQQRREL
                     IQARLDHLNLEEIMPAYEVTLKSVDVLTVAAIREMVPAVEVMSQRCGELFKMIADWMV
                     ANGLPFGAPMTVYHNKSYTRENIDTECAFIIPGVAGNKIALPPSPIVVRQMEAIPHAA
                     TTIVADNFYQKADGLTPAYHALAQWIEDNDYRIVGAPRELYYGSPERGDFTAEIQFPV
                     EKAE"
     gene            2558..3535
                     /locus_tag="PKL11_10140"
     CDS             2558..3535
                     /locus_tag="PKL11_10140"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNZ14000.1"
                     /translation="MESRRYRSLFWPVILIGIGVLWLLGNLGIIEPVSLGGLLRFWPV
                     LLIVAGLDMLLTRRSTWVGALMGLLVIGIIGFVLIAGPGLGWVQTPSAKTEQFTAELN
                     DAAAATIALDLAEYPAEVNALTDDDLLFDATLDHHGVAVFTDTGSAQRVLRLAYTATP
                     GTLFSFGMNEDWQIGLTPQIPLALNVDAGSGPATLNLADLNLTYLGVDAGSGPFTLML
                     PASPDTYALELDAGSGPVVMDVAPDADLTMTINCGSGPLTIDLPNDAAVRFEVREDGS
                     GPVTTPGWLPRTQHGEDDEGVWETDGYATATHRVLIIIEDQGSGPVIIK"
BASE COUNT          831 a         1018 c         1009 g          861 t
ORIGIN      
        1 gtcagcacac catccataca gccttccaat caaaatatgc gatttattga atgtctatca
       61 ttcaaaataa tgggcagctc aatcaagcgc tgccccaaaa tacttatgca gcctgcttga
      121 gccaggcagc aatttcagcc tcagtcggga tccgccccga ggcaaccacc tgcccgttga
      181 tcaccaggcc cggggtggac agaatcggcc atttcataat ctcggccata tcagtcactt
      241 tttcaatttc agcttcaagc tgcagctcgg caaccacttt gcgagccgcg gcttccacgc
      301 gcttgcagtt ggcgcagcca gggcccaaaa ctttgatatt aatcattgca aaacctccgt
      361 cacggtttaa ggatgctttc ttttggccgc acattgcggg cagggacaat ccgtcccggt
      421 gattgttttt gccggcgccg gtctttctcc ggtcatttca gccactacat ctatcagttg
      481 atagacttcg gggcgcgaca cccggtaaaa gatattccag ccatcccggc ggtcctggat
      541 cagcccggct tcgcgcagca cagccaactg ctgagagatg tacgcctggc gataacccag
      601 gtgcgcttcc atgtggcaga cgcaatgctc gccgtcgcgt aaaatctcca gaatggccag
      661 gcgggctggg tgcgtcagcg ccttgaagat ctgagcctgg cgttcaaggg gctgggtatg
      721 ggttacaaca ttcaattcca tgaatatgat tctaaggcca gtgtctacgg ctgtcaagag
      781 tgcaatgatg ttgagccacg gctttacaag actggatggc tggtgaacac aggaagttaa
      841 ggatcgcgga gcatccttgc aggacgcacg cggccctgta agggtcgcgc agcaccctta
      901 cagaaccgca tagcgtcatg agagggtata attaatctgt cgtggacaga gctaaatgcc
      961 gtacagcgga ggaagaaaaa tcaaaaccat accccgcaaa ataaaaaaac agaaaggttg
     1021 atgccagcga atggagggta aaaacgcaag ctcacaaatc ggggtgcaga gtggatccgt
     1081 aacaaggaac catatcctgt ggctgttata cagcgcagct tttgggttag cgctggttat
     1141 tcaaatttta tccagcgcac taacctggta tcgcactatt acctatgatt atttctgcgg
     1201 caatccgctg atgggtgact gggcactgtg gtttgtgatc tcgctaatcc tgggcgcact
     1261 ggtcatcccc ggcggaatct ggtcaatccg gcacagaaaa caacgtatgg ggatcattta
     1321 cagggtgggc tctttgcttt atctcttgag tgtggtcgtt atgttgacag ccggcatctt
     1381 ttggggaact gtgccagatt tcttgaattt cattggctat gcatttggat aattgcctat
     1441 aacaggcaac gacttcacgc gtcttgggcc gcagcagatg tgtttttatt catccggcag
     1501 ggctggaaga gtgttaggaa aataaattcg ttgaaaatca ccccttgact ctagggttac
     1561 ccgaggcatt acactgtgag catgttccaa ataggcgagt tttcccgaat tgcctgtgtg
     1621 acaatcgaca cactacggca ctatgacgcg cttggtttgc tcaaacccgt gaaagtggat
     1681 ccgatcacgg gctaccgcta ctacagcgca aagcagctgc agccgctcaa ccggatcatc
     1741 gcgctgaaag agatcgggtt atctttgaaa gaaattgcgc taattcttca ggataacctc
     1801 tccatagatg agctgcgtgg catcttgaaa gtgcagttgg ctttagctga aagcgttatc
     1861 gaggcaaccc aacagcgccg ggagttaatt caggctcgcc tagatcatct caatctggag
     1921 gaaattatgc ctgcctacga agtaactctg aaatccgtgg atgtactcac ggttgccgcc
     1981 attcgagaaa tggtgccggc agttgaggtc atgtctcaac gttgcggtga gcttttcaaa
     2041 atgattgccg attggatggt ggccaatggt ctgccctttg gcgcccccat gactgtgtat
     2101 cataacaaaa gctatacccg cgagaatatt gacaccgaat gtgctttcat catcccgggt
     2161 gttgcgggca acaagattgc cctgccgccc agcccaatcg ttgtgcgtca aatggaagcc
     2221 attccacacg cggccactac cattgtcgcg gacaatttct atcagaaggc ggatggcctg
     2281 acaccagcct atcatgcgct ggcgcagtgg atagaagaca acgattatcg cattgttggc
     2341 gcaccccgag aactatatta cggctcccca gagcggggag actttaccgc tgaaattcag
     2401 tttcctgttg aaaaagccga ataggcatcg gcgccaccgc tgcattcatt gatgccatgg
     2461 atcaatcccg atcacccgtg cgcccggaac ctgcgtgaaa cctcgtgcca ctttcaacgt
     2521 atatggatat gaaattcttt gatttgggag tatcacgatg gaatcacgac gctatcgctc
     2581 attattttgg ccggtaatat taatcggcat tggtgtctta tggctgctgg gcaacctggg
     2641 catcatcgaa cccgtctcgc tgggcggact gctgcgcttc tggccggtgc tgttgattgt
     2701 ggccgggctg gatatgctgc tgacacgccg ctcgacctgg gtcggcgcgc tgatgggcct
     2761 gctggtcatc ggcattattg gctttgtgct gattgccggg ccaggcctgg gctgggtgca
     2821 gacccccagc gccaagaccg agcagttcac cgcggagctc aacgacgcgg ccgcggccac
     2881 cattgccctc gatctggcgg agtaccccgc cgaagtcaac gccctgacgg acgatgattt
     2941 gctcttcgat gccacactcg atcatcacgg cgtggccgtc ttcaccgata ccggctcggc
     3001 gcagcgggtg ctgcggctgg cttacacggc cactcccggc acgctgtttt ccttcggcat
     3061 gaacgaagac tggcagattg gcctcacccc tcagattccg ctggcgctga acgtggatgc
     3121 gggctctggc ccggccacct tgaacctggc agacctgaac ctgacctacc tgggtgtgga
     3181 tgctggctcc ggcccattca ccctgatgct gcccgcctcc cccgacacct atgcgctgga
     3241 gctggatgcc gggtctggcc cggtggtgat ggatgtggcg cccgatgccg atctcaccat
     3301 gacgatcaac tgcggctctg gcccgctgac cattgacctg ccgaatgacg ccgccgtgcg
     3361 ctttgaagtg cgcgaagacg gctctggccc cgtgaccacg cccggctggc tgccccgcac
     3421 ccagcacggc gaagatgacg aaggcgtttg ggaaaccgac ggctacgcca ccgccaccca
     3481 ccgggttttg attatcatcg aagatcaggg ctctggtccg gtgattatca agtaggtttt
     3541 agcaatctgg ttttggtaaa aacatcccaa gggccattag acctttggga tgtttgcttt
     3601 tcccgggtgc agcggcggga caaaaatccc cgcgcgcggg gccaggaatc gtggtatact
     3661 cattaaaccg tattgaccag gacgagtagc cccgtcagtg cccttagaga gcagaagtc
//
LOCUS       DAOPVS010000178         3678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_25521_length_3678_cov_56.999448, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000178 DAOPVS010000000
VERSION     DAOPVS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25521_length_3678_cov_56.999448"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKL11_10145"
     CDS             complement(<1..746)
                     /locus_tag="PKL11_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ14001.1"
                     /translation="MKIAVITDIHANLLALNAVLEDIADQKPDQVICAGDMVGTSAYP
                     DAPQVWQTLQERGIPCVLGNEEARIINFHGPEPDACWRDSVQFRPLQYRARQFSSDDV
                     AALSELPITILVEGPHHQNVLVCHASPGSLHRSPFDGIDPSMAQALQSVPASVIVVGH
                     YHTRWHYHLQDQFIIMAGSVGLPFRGLLGQAEYLLLTCHGQTWDYEYRTVPYDHPAAL
                     ARVLNSTFIEQGGPIGWLMLDEALTQQDRLS"
     gene            complement(761..1420)
                     /locus_tag="PKL11_10150"
     CDS             complement(761..1420)
                     /locus_tag="PKL11_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNZ14002.1"
                     /translation="MSDQEIEAKFYLSDLPAVQRRLLLLGAQLSQPRLKETNLRLDTP
                     SGDLSAAGRTLRLRQPAPSTCNASRSDWQDDSRAAVLTYKGPQQANAEVAVRQELEVV
                     VSDVEAARRLLEALGYPVMVTYEKWRTAYRLLGVEVSLDELPYGNFVEIEGPDVARIH
                     TAADRLGLNWEKRIPASYLSLFYSLKRKLGLPMPNLTFAAFERRGITAADLGVEPADA
                     A"
     gene            complement(1465..1884)
                     /locus_tag="PKL11_10155"
     CDS             complement(1465..1884)
                     /locus_tag="PKL11_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14003.1"
                     /translation="MKPRRGHWLVMIGLFLSAGVGWARLIQALLSWQWLVTYGAEPGP
                     LYIAITGGLIGAAGLVAALGLLLRRPWGAPWVRGYALALALFTWADRLLFSRSTAAWA
                     GWPIDLILTLLLLVAAWWLLRPQKSMPIPPPANSKNQ"
     assembly_gap    1971..2070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2071..3656)
                     /locus_tag="PKL11_10160"
     CDS             complement(<2071..3656)
                     /locus_tag="PKL11_10160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ14004.1"
                     /translation="MDTAPAESTPIETPSVQPPRRRLTLPRDPALWLLLAAVLIYLVT
                     RFVGLDRFPIYFFTDEAVQTILADDFLDQGLKSYFDEVLPTYFVNGGQYNLGTSVYLQ
                     VLAAWLPRSVWVTRGVSIIVSLLAALGIGLSLRNIFKDRFAWTGVLLLTITPIWFLHS
                     RTAFEVVEATAFYAGFAYCYLMYRHVNPRYLLAAAVLAGLMFYSYSPAQLVLVTTLVL
                     LFFSDLRYHWQNRKYVLISLGIGAVCTIPYIRFLINHPTENRRHLEILHSYWIQSIPF
                     SEKLDIYLREYRRGINPFYWFAPNPPDLVRHVMKGYGHLWLPTLPLLVIGLWQAIKKF
                     KQSAYRTVLIMLVAAPTGGALAQSTALRALFYVIPAVLLMALGLNWLLEKLARPRWAY
                     VLVTVLIFALLAGSSFYMLNDSLTNGPLWFDDYGMGGMQYGGEQLFSAVAEYQAENPG
                     LKLTVTPIWANGTDVIARFFAGDPLPFEMGSVVGYIDYKLPLDDTLAFVVVPQEYKQL
                     YESGKFAEIQVDRTLYYPNG"
BASE COUNT          739 a         1017 c         1225 g          597 t
ORIGIN      
        1 gacaggcggt cttgctgcgt cagcgcctca tccagcatca accagccaat ggggccgccc
       61 tgctcaataa atgtgctgtt caacacccgg gcaagcgccg ccggatgatc atagggcacg
      121 gtgcggtatt cgtaatccca ggtctgaccg tgacaggtca gcagcaggta ttcggcctgc
      181 cccagcaagc cccggaaagg cagccccacg ctgcctgcca taataatgaa ctggtcttgc
      241 aggtgataat gccagcgggt gtgataatgc cccacaacaa tgacactggc aggcacggat
      301 tgaagcgctt gagccatcga tgggtcaatc ccatcaaaag ggctgcggtg caggctgccc
      361 ggcgaggcgt ggcagaccag cacattctgg tggtgcgggc cttcaacgag gatggtgatg
      421 ggcagctcgc tcagcgcggc aacatcatct gaggagaact ggcgggcgcg gtactgcagc
      481 gggcggaact ggacggaatc gcgccagcag gcatctggct caggcccgtg aaaattgatg
      541 atgcgcgctt cctcgttgcc cagcacacag gggatgccgc gttcctgcaa ggtctgccag
      601 acttgcgggg catcggggta ggcgctggtg cccaccatat cgccggcgca aatcacctgg
      661 tcgggttttt ggtcggcaat atcttccagc accgcgttga gggccagcag gttggcgtga
      721 atatcggtga tgacggcgat cttcataccg gggcagggct tcaggcggcg tctgccggtt
      781 ccacacccag gtcggcggcg gtgatgccgc ggcgctcaaa ggcggcaaag gtcaggttgg
      841 gcatgggcag ccccagcttg cgtttgaggc tgtaaaacag cgacaggtag ctggccggga
      901 tgcgtttttc ccagttcagg cccaggcggt cggcggcggt gtgaatgcgg gccacgtcgg
      961 ggccttcgat ttccacaaaa ttgccgtagg gcagttcatc cagcgagacc tcaaccccca
     1021 gcagacggta ggccgtgcgc cacttttcgt aggtcaccat cacggggtaa cccagcgctt
     1081 ccagcaggcg gcgggcagcc tccacatcgg agacgaccac ttccaattcc tggcggacgg
     1141 cgacttcggc gttggcctgc tgcggaccct tgtaggtcag cacggcggcg cggctgtcat
     1201 cctgccagtc ggagcgggag gcgttgcagg tggagggggc aggctggcgc aggcgcaggg
     1261 tgcgcccggc agcgctcagg tcgccggaag gggtatccag gcgcaggttg gtttctttga
     1321 gccggggctg actgagctgt gcgcccagca gcagcaggcg gcgctgcacg gcgggcaggt
     1381 cgctgaggta aaatttggct tcgatttcct ggtcactcat gggggttatc tttgtggggg
     1441 tggactgaaa acgatactct aattttactg atttttagaa ttcgcgggcg gcggaatggg
     1501 catggatttc tgggggcgca gcagccacca ggccgcgaca agcagcagca gggtcaggat
     1561 caggtcaatg ggccagccag cccaggcggc agttgagcgc gagaacagca gccggtcggc
     1621 ccaggtgaag agagccagcg ccagggcata gccgcgcacc cagggcgccc cccaggggcg
     1681 gcgcagcagc aggcccagcg cggcgaccaa tcccgcggcg ccgatcagcc cgccggtgat
     1741 ggcgatgtag agcgggccgg gttcagcgcc gtaggtcacc agccactgcc agctcaacag
     1801 ggcctgaatc aaccgcgccc agcccacccc ggcggacaga aacagcccga tcatcaccag
     1861 ccaatggccg cgccgcggct tcatcgaagc gcaatttccc agacgtgcac atgggtgggt
     1921 tcaacatcat tgaggctcaa aatttcaatc gtcaggcggc tgacgggcag nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgccgttgg ggtagtacaa cgtgcggtcc
     2101 acctgaatct cggcgaactt gccgctttcg taaagctgtt tgtattcctg gggaacgacc
     2161 acaaacgcca gggtgtcgtc cagcggcagc ttgtaatcaa tgtacccgac cacactgccc
     2221 atctcaaagg gcagcgggtc gccggcaaag aagcgcgcaa tcacatcggt gccgttggcc
     2281 cagatgggcg tgaccgtcag cttgaggccg gggttctcgg cctgatattc ggccaccgcg
     2341 ctgaaaagct gttcgccgcc gtactgcatg ccgcccatgc cgtaatcatc aaaccagagc
     2401 gggccgttgg tgagcgaatc gttgagcata tagaagctgc tgcccgccag cagcgcaaaa
     2461 atcagcaccg tgacgaggac gtaagcccag cgcggtcggg cgagcttttc cagcagccag
     2521 ttgagcccca gcgccatcag cagcaccgcg ggaatgacat agaacagcgc ccgcagcgcc
     2581 gtggactgcg ccagcgcgcc gccggtaggg gcggctacca gcataatcaa cacggtgcgg
     2641 taggccgact gcttgaattt tttgatggcc tgccacagcc caatgaccag cagcggcagg
     2701 gtgggcagcc agaggtggcc gtagcccttc atcacatggc gcaccaggtc gggcgggttg
     2761 ggcgcaaacc agtagaaggg gttgatgccg cggcgatatt cacggaggta gatatccagc
     2821 ttttcgctga aggggatgga ttggatccag taagagtgca aaatctcgag gtggcggcgg
     2881 ttttcggtgg ggtggttgat gaggaagcga atgtagggga tggtgcagac tgcgccgatg
     2941 ccgagggaaa tgaggacgta cttgcggttc tgccagtgat agcgcaggtc ggagaagaac
     3001 aacagcacca gcgtcgtcac aagcaccagt tgggcggggc tgtagctgta gaacatcagg
     3061 ccggccagca cggccgcggc cagcagatag cgcgggttga cgtggcggta catcaaatag
     3121 cagtaggcaa aaccggcgta gaaggcggtg gcctccacca cctcgaaggc agtgcgggag
     3181 tgcaggaacc agatgggcgt gatggtcagc aggagcacgc ccgtccaggc aaagcggtct
     3241 ttgaagatat tgcgcagcga caggccgatg cccagcgcgg ccagcagcga gacgatgatg
     3301 ctgacgccgc gcgtcaccca gaccgagcgc ggcagccagg ccgccagcac ctgcaaatag
     3361 accgaggtgc ccaggttgta ctgcccgccg ttgacaaaat aggtgggcag aacttcatcg
     3421 aagtagcttt tcaggccctg gtcgagaaaa tcatcggcga ggatggtttg cacggcctca
     3481 tcggtaaaga agtagatcgg gaagcgatcc aggccgacga agcgcgtgac cagatagatg
     3541 agcactgccg ccagcagcag ccagagggcg gggtcacgcg gcagggtgag gcggcggcgc
     3601 gggggttgaa cagacggggt ttcgatgggt gtggattcgg caggggcagt gtccatagca
     3661 aatcctgtgc ggggtggg
//
LOCUS       DAOPVS010000179         3653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_25754_length_3653_cov_44.206782, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000179 DAOPVS010000000
VERSION     DAOPVS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25754_length_3653_cov_44.206782"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="sufC"
                     /locus_tag="PKL11_10165"
     CDS             complement(<1..718)
                     /gene="sufC"
                     /locus_tag="PKL11_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNZ14005.1"
                     /translation="MADLIIRDLHVSINDKEIIKGFTLTIPQGEVHAIMGPNGTGKST
                     LAYTLMGHPNYTVTSGEVIFNGQNILDLEPDERSRLGLFLAFQYPVAIPGVTLANFLR
                     SAVNARRRAENPADKGIPVPEFRRMLKEKMAMLKMDPAFAGRYLNDGFSGGEKKRAEI
                     LQMAVLQPQIAVLDETDSGLDIDALRIVSEGVNTLRGPGLGVLVITHYQRILNYIRPD
                     VVHVMMGGRIVESGGADLALH"
     gene            855..1487
                     /locus_tag="PKL11_10170"
     CDS             855..1487
                     /locus_tag="PKL11_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNZ14006.1"
                     /translation="MNSTREHVLQTLLEHPHSTVSDLAEAVGINMISVRHHLNSLQAE
                     GLIAVEEERHGVGRPRLRYSLTEQGRERFPTHYLRLTNRLLEQIKESLPGPVVHRLFA
                     ELAGDIAGRYTARFAKMSFEERLNALKNILAEEGFVISWDQEGDTYRIHEIVCPYYQI
                     GQKHPEICILDQTLISKLLAVPIEKVQCVLHGAEHCTYLINQTAKSEETA"
     gene            1484..1831
                     /locus_tag="PKL11_10175"
     CDS             1484..1831
                     /locus_tag="PKL11_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNZ14007.1"
                     /translation="MRATPGTNPAVWESEAEQPELCAKLREALSAVADPEIGLNIIQL
                     GLIRNVKIEADQAIIRMILTTPFCPYGPAMLEETRQKAEAALERPVFMDLGMEAWDFG
                     MMTEDLGADWGLY"
     gene            complement(1823..2212)
                     /locus_tag="PKL11_10180"
     CDS             complement(1823..2212)
                     /locus_tag="PKL11_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNZ14008.1"
                     /translation="MPLHPETAENLERLDQLIARLPQAFVPEKAAGVDLTGVLEVHYL
                     DLDNQEQISCWTLIIKNQTCHTIEGDADQPGLRLICRLADLAAVAERQLDPLSAFMQG
                     KFTLKGSFASAMRLPPLFRINLPAGSV"
     gene            complement(2215..2568)
                     /locus_tag="PKL11_10185"
     CDS             complement(2215..2568)
                     /locus_tag="PKL11_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNZ14009.1"
                     /translation="MPTYTMDQIMEKLSGRLMLQQAAGLNATILLTVKQAGTVKSEWV
                     IVIKDQTSDVKKIEGAVEKPVNPDLTFSGEDQDLVAVFSGQLDPVRAFMMGKFTMKGN
                     MALAMRLPMLFKTTD"
     gene            2672..3649
                     /gene="corA"
                     /locus_tag="PKL11_10190"
     CDS             2672..3649
                     /gene="corA"
                     /locus_tag="PKL11_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNZ14010.1"
                     /translation="MIRSVCFHKDGSQVRNLTPEAMRSALAEPQSLLWVSLECPSDLE
                     VQNTLNDVFHFHPLAIEDCLSTGYQTPKIDDFGEYLFIVVHAVEEHQDFAELETRELD
                     LFLGKNYLITIFRDDGTSPLDSMWKRIERDERLHQFGADFLCHAVLDEVVDEYLPLID
                     KMEEEIDQLEDRVLARPEPQTLSRILDLKHAIMSLRRIISPQREVMNRLSRDDFRLID
                     RQSRIYFRDIYDHLVRIQDMTESLRDIIGGDLDIYLNSTSLRLNEVMKALTIVSTIFL
                     PLSFVAGVYGMNFHYMPELSKTWGYPMAWGIFVLIVVGMVTFFKKRKWF"
BASE COUNT          823 a          873 c         1094 g          863 t
ORIGIN      
        1 gatgcagggc caggtcggcg ccgccggatt caacaatccg accgcccatc atgacatgga
       61 ccacatcggg gcgaatgtag ttgaggatgc gttgatagtg ggtgatgacc agaacgccaa
      121 ggcccgggcc gcgcagggta ttgacgcctt cggagacgat gcgcagggcg tcaatatcga
      181 ggcctgagtc ggtctcatcc agcacggcga tttggggctg caggacggcc atttggagga
      241 tctcggcgcg tttcttttca ccgccggaga agccatcgtt taaatagcgc ccggcaaagg
      301 cggggtccat tttgagcatg gccatttttt ctttgagcat acggcggaat tctggcaccg
      361 ggataccctt gtcggcgggg ttttcagcgc ggcggcgggc attgacggct gaacgcaaaa
      421 agttcgccag cgtgacaccg gggatggcga ccgggtactg gaaggccagg aacaggccca
      481 ggcgggagcg ttcatcgggt tccaggtcaa ggatattttg accgttaaag atgacctcac
      541 cgctggtgac ggtgtagttg ggatgcccca tgagggtgta ggccagggtg gatttcccgg
      601 tgccgttggg ccccatgatg gcatgaacct caccctgtgg gatggtgagg gtgaatcctt
      661 tgatgatttc cttgtcgtta atactgacgt gcaggtcgcg aataatcagg tctgccatac
      721 tcaaactact ccttgagttt gttacgattg gtcaaattgc agcgacatta tatcacaccc
      781 ggccgcgaag gtcaatattt atcataaata tataaaatat tgactaattc aaatttatcc
      841 gttatactgg cggtatgaac agtacgcgcg aacatgtgtt gcaaaccctg ctggaacacc
      901 cccactcaac ggtcagtgac ctggctgaag cagtggggat caatatgatt tcggtgcggc
      961 accacctcaa cagcctgcaa gctgaggggt tgattgcggt ggaggaggag cgccacggcg
     1021 tggggcggcc gcggctgcgc tactcgctga ccgaacaggg ccgcgagcgc tttccaaccc
     1081 attacctgcg cctgaccaac cggctgctgg aacaaatcaa agagtcgctg ccggggccgg
     1141 ttgtccaccg gttgtttgcc gaactggcgg gtgatattgc cgggcgatat acggcccgat
     1201 ttgccaaaat gagctttgag gaacggttga atgcccttaa aaatattctg gcagaagagg
     1261 ggtttgtgat ttcatgggat caggaaggcg atacctaccg aattcacgag attgtctgcc
     1321 cctactatca gattgggcag aagcacccgg aaatttgtat actggatcaa accctgatat
     1381 ccaaattact ggcggtgccg attgaaaaag ttcaatgcgt gctgcacggt gctgaacact
     1441 gcacctatct cattaatcag actgccaaaa gcgaggaaac agcatgagag ctacccctgg
     1501 taccaacccg gctgtgtggg aatctgaggc tgaacagccc gaattatgcg ccaagctgcg
     1561 cgaagcgttg agtgcggtcg ccgaccctga gattgggctc aacatcattc agttggggtt
     1621 gatccgaaat gtgaaaattg aggcggatca ggcgatcata cggatgattt tgacgacgcc
     1681 gttctgcccg tatggaccag ccatgctgga agagacccgg caaaaagctg aagccgcgct
     1741 ggaacgcccg gtttttatgg acctgggcat ggaagcctgg gattttggga tgatgaccga
     1801 ggacctgggc gcggactggg gcctatactg agccagcggg gagattaatc cgaaagagcg
     1861 gggggaggcg cattgccgat gcaaagctgc ctttcagggt gaacttgccc tgcatgaatg
     1921 cgctgagcgg gtcaagctgc cgttctgcaa cagccgccaa gtctgccagg cggcagatca
     1981 ggcgcagccc gggctggtcg gcatcacctt cgattgtgtg gcaggtttga tttttgatga
     2041 tgagcgtcca gcaggaaatc tgctcctgat tgtccaggtc caggtaatgg acttccagga
     2101 cgccggtaag atccactccg gcggcctttt cggggacaaa agcctgtgga agtctggcaa
     2161 tcagttggtc gaggcgctcc aggttttccg ctgtttcggg atggagcggc atggtcagtc
     2221 ggtggttttg aagagcatgg gcaggcgcat ggccagggcc atgttgcctt tcatggtgaa
     2281 tttgcccatc atgaaggcac gcacggggtc gagctggcca gaaaagaccg ccaccaggtc
     2341 ttgatcttca ccgctgaagg tcaggtcggg gttgactggt ttttcaacgg caccttcgat
     2401 tttcttgaca tcgctggtct ggtctttaat cacaatcacc cactctgact ttacggtacc
     2461 agcttgttta accgtcaata agatggtggc attgagccca gcagcctgct gcagcatcag
     2521 ccggccgctc aatttttcca tgatttgatc cattgtgtaa gttggcatgg ggaaggtcct
     2581 cctgcgttaa attatacgcc agaaggggaa aaaggatata ctagaggggt tagaagttgc
     2641 ggcttttaat tcaacggcca ggagcaattt tatgattcgt tcggtatgtt ttcacaaaga
     2701 tggcagccag gtacgcaatt tgacacccga ggccatgcga agcgcactgg cagagccaca
     2761 atcgctgctg tgggtcagcc tggagtgccc atctgatctt gaagtacaga ataccctgaa
     2821 tgatgtcttt cattttcatc ctctggcgat tgaagactgt ctgagcaccg gctatcagac
     2881 gcccaagatt gacgattttg gcgagtattt gtttatcgtg gttcatgcgg ttgaggagca
     2941 tcaagatttt gcggagttgg agacgcgcga actggatttg tttctgggga agaattacct
     3001 gattacgata ttccgggatg atggcacttc gccgctggac agtatgtgga agcgaattga
     3061 gcgggatgaa cgactgcacc agtttggcgc cgattttctc tgccatgccg ttttggatga
     3121 ggtggtggac gaatacctgc cattgattga caaaatggaa gaagagattg accaactgga
     3181 agaccgagtg ctggcgcggc ctgaacctca gacattgagc cggattctcg atttgaaaca
     3241 tgcaattatg tcgctgcggc ggattatctc gccacagcgc gaggtgatga accggctgag
     3301 ccgggatgat ttccggctga ttgaccgcca gagccggatc tacttccggg atatttacga
     3361 tcatctggtg cggattcagg atatgaccga gtcgctgcgg gatatcatcg gcggcgacct
     3421 ggatatttac ctcaattcaa catcactgcg gttgaatgag gtgatgaagg ccctgaccat
     3481 tgtctcgacc attttcctgc cgctctcttt tgtagcgggc gtgtatggta tgaatttcca
     3541 ctacatgcca gagctctcca aaacctgggg gtaccccatg gcctggggca tttttgtact
     3601 gattgtggtg gggatggtga cgtttttcaa aaagcgcaag tggttttaga gaa
//
LOCUS       DAOPVS010000180         3606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_26217_length_3606_cov_63.320755, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000180 DAOPVS010000000
VERSION     DAOPVS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26217_length_3606_cov_63.320755"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKL11_10195"
     CDS             <1..360
                     /locus_tag="PKL11_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HNZ14011.1"
                     /translation="GALSGGNQQKVIIAREFSRPIQFLVASQPTRGLDVGSIEYIHGR
                     IIQKRDEGCAVLLVSPELDEVMELSDRIAVMFRGKIIAVVAADQVTKEEVGLLMAGIM
                     PENLGQRTGPRQVETTL"
     gene            379..1662
                     /locus_tag="PKL11_10200"
     CDS             379..1662
                     /locus_tag="PKL11_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ14012.1"
                     /translation="MPEQKPQPKPDVGKELLKEVTRPPKGRTFWARAGNALLIPALAV
                     LTGLLLGAVIIILTSEAVYQGFQQSFGAGMLAVWKSIADAYGAMFTGAFGNPVKFFTA
                     LGSGEAIQIRRAINPPLESLVVSTPYIFAGLAVALGFRAGVFNIGVEGQLFMGAVAAT
                     YVGYAVKGLPPYIHMPLAFLAGAAGGAIWGFIPGWLKAKTGGHEVINTIMLNWIAFRL
                     TDWLLAGPMQRPDSGGMPISPVIETSAQIPQFFGTPIRFHLGFFIALAVAFGVYWLLF
                     KTTWGFELRSVGANPSGGRYAGMNIARNTILAMALSGALAGMAGANQVLAVTRSMAMG
                     LSSGYGYDSIALALLGNSSPLGVVLASLLFGFLRNGASQMMVVSRIPIDIISIIQAFI
                     LIFIAAPAIIRSLYRMKQAKDADTGVVLSSWGGGK"
     gene            1662..2930
                     /locus_tag="PKL11_10205"
     CDS             1662..2930
                     /locus_tag="PKL11_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ14013.1"
                     /translation="MTATVLRRRLGETSPVRQRVMGIVFLVLAVLIYLLFIRTTTGDL
                     LTTFVMTPGGITQGVAPDWILPAQTTLYILAVVCAVLGGVQLARGFGRYTNWILGIVA
                     LFFVFGFIVWAAAGQSLNLGGMLSNAVLRAVPITLGALSGLLCERSGIINIAIEGMML
                     MGAMLGALVGSVTGSMWIGLLAAIAGGVLMAWVLAVLSIKYKINQIISGTVINIFATG
                     FTSYISAKFLQTYQHLNNGPIFPRWPIPLLADIPVIGPIFFNTNFFVYAMFILLIVLQ
                     IALFSTRWGLRLRSVGEHPKAADTLGINVFKTRYMATLLGGAVAGLAGAYFTLGSVGR
                     FDEMMSAGRGFIGLAAMIFGNWMPFGSFGAGMLFGFADTLGSKLTILGSTIPPEFMAM
                     LPYITTMVVLAGVVGRGHAPAAEGTPYEKE"
     gene            2934..3335
                     /locus_tag="PKL11_10210"
     CDS             2934..3335
                     /locus_tag="PKL11_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNZ14014.1"
                     /translation="MASATVRWIGGKQFVGTDSTQHSVVISTPDEGIGMKPSELLLVA
                     LASCTAVDVVEILHKKRLTLESLEIQASAEQDSEPPWAFRKIQVHYVLHGSGLTDTAV
                     SQAIQLSEEKYCSVAATVRGVAEVHTSFEIV"
     gene            3407..>3606
                     /locus_tag="PKL11_10215"
     CDS             3407..>3606
                     /locus_tag="PKL11_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14015.1"
                     /translation="MAKPASNAPKFMSLRLKLLLPILALLTVIFIVAYVGLSAFLNNT
                     VFGILDEESKTIIDSVTACLDG"
BASE COUNT          626 a         1093 c         1043 g          844 t
ORIGIN      
        1 ggggcgcttt cgggcggcaa ccagcaaaaa gtgatcattg cccgcgagtt ctcccgcccc
       61 attcagttcc tggtggcctc gcagccaacc cgcggcctgg atgtgggctc aatcgagtac
      121 atccatgggc gcatcatcca gaagcgtgat gaaggctgtg ctgtcctgct ggtctctcct
      181 gagttggacg aagtgatgga gctttccgac cgcatcgccg tcatgttccg cggcaagatc
      241 attgctgtgg tcgcggccga tcaggtgacc aaagaagaag tcggcctgct gatggccggc
      301 attatgcccg aaaatctggg ccaacggact ggcccgcgcc aggtggaaac caccctgtag
      361 aagtggaagg atatcaccgt gcctgaacaa aaacctcaac ccaaacccga tgttggtaag
      421 gaattgctga aggaagtcac gcgcccgccc aagggccgca ccttctgggc gcgtgcgggc
      481 aatgcgctgc tcatcccggc gctggcagta ttaactggcc tgctgcttgg tgccgtcatc
      541 atcatcttga ccagtgaagc cgtgtaccag ggcttccagc agtcattcgg cgctggcatg
      601 ttggctgtct ggaaatccat tgccgatgcc tacggcgcca tgttcaccgg ggcctttggc
      661 aacccggtca agttcttcac ggccctgggc agcggcgagg cgatccagat tcgccgggcc
      721 atcaacccgc cgctggagag cctggtggtc agcacgccct acatcttcgc cgggttggct
      781 gtggcgttgg gcttccgggc tggcgtcttc aacatcggtg tggaaggcca gctcttcatg
      841 ggcgctgtgg cagccaccta tgtgggctat gccgtcaagg gcctgccgcc ctatatccac
      901 atgccgttgg ctttcctggc tggcgcagcg ggtggggcaa tctggggctt catccccggc
      961 tggctcaagg ccaaaaccgg tggacatgag gtgatcaaca ccatcatgct caactggatt
     1021 gccttccgat tgaccgactg gctgcttgcc ggtcccatgc agcgccccga ttcgggcggc
     1081 atgcccatca gcccggtcat cgaaaccagc gcccaaatcc cgcagttttt tggcaccccc
     1141 atccgcttcc atctgggctt tttcattgcc ctggcggtgg cctttggggt gtactggctg
     1201 ctcttcaaaa ccacctgggg ctttgaactg cgcagcgtgg gtgccaaccc ctcgggcggc
     1261 cgctatgcgg gtatgaatat cgcccgcaat accattcttg ccatggcgct ctcgggtgct
     1321 ctggcgggca tggcaggtgc caaccaggtg ctggcagtca ctcgcagcat ggccatgggc
     1381 ctgtcctccg gctatggcta tgacagcatt gccctggcgc tgctgggcaa cagctcgccg
     1441 ctgggcgtgg tgctggcttc cctgctattt ggcttcctgc gcaacggcgc ctcgcagatg
     1501 atggttgtct cgcgcatccc gattgatatt atctccatca tccaggcctt cattctgatc
     1561 tttatcgctg ccccggcgat catccgctcg ctctatcgta tgaagcaggc caaagacgcc
     1621 gatacgggtg ttgttttgag cagttgggga ggaggtaaat aatgaccgcc accgttctac
     1681 gccgccgcct gggcgaaacc tcgccggttc gccaacgcgt gatggggatc gtgttcctcg
     1741 ttctggccgt gctgatctat ttgctgttca tccgcaccac aactggcgat cttctgacta
     1801 cctttgtgat gacccctggt ggtatcaccc agggtgtggc ccccgactgg attttgccag
     1861 cccagacaac gctttatatc ctggcggtgg tttgcgccgt ccttggcggc gttcagctcg
     1921 cccgcggctt tggccgctac accaactgga ttttggggat cgtcgcgctg ttttttgtgt
     1981 tcggcttcat cgtctgggcc gcagcgggtc aatctttgaa cctgggcggc atgttgagca
     2041 acgctgtgct gcgcgccgtg cccattacct tgggggcgct gtctggcctg ttgtgcgagc
     2101 gctcgggcat catcaatatt gccattgaag gcatgatgtt gatgggcgcc atgctgggtg
     2161 ccctggtggg cagtgtgacc ggcagcatgt ggattggcct gctggcagcc attgccggcg
     2221 gcgttttgat ggcctgggta ctggctgttc tttccattaa gtataagatc aaccagatta
     2281 tttctggcac ggtcatcaat atctttgcca ccggtttcac ctcatacatt tcggccaaat
     2341 tcctgcaaac ctaccagcac ctcaacaacg ggcccatttt cccgcgctgg cctatccccc
     2401 tgctggccga tatccccgtg attgggccaa ttttcttcaa caccaacttc ttcgtctatg
     2461 ccatgttcat cctgctgatt gtgctgcaga ttgcgctgtt ctccacccgc tggggtctgc
     2521 gcctgcgctc ggtgggtgaa caccccaagg ctgccgacac gctgggcatc aacgtcttca
     2581 aaacccgcta catggccacc ctgctgggcg gtgcggtggc gggcctggcg ggcgcctact
     2641 tcaccctcgg ctcggtgggc cgttttgatg aaatgatgtc tgccgggcgc ggctttattg
     2701 gcctggcagc gatgatcttt ggcaactgga tgccctttgg ctcgtttggc gccggcatgc
     2761 tctttggctt tgccgatacg ctgggctcca agctgaccat ccttggcagc accatcccgc
     2821 cagagtttat ggcgatgctg ccttacatca ccaccatggt tgtgctggcg ggtgtggttg
     2881 ggcgcggaca tgcccccgcc gccgaaggca caccgtacga aaaggagtaa atcatggcga
     2941 gtgcaaccgt tcgctggatt ggcggcaagc agtttgtcgg caccgattca acgcagcatt
     3001 cggtggtcat ctccacacct gatgagggca tcggcatgaa gccctcagaa ctgctgctgg
     3061 tggcattggc ctcctgcact gccgtagacg tggttgagat cctgcacaaa aaacgcctga
     3121 cgctcgaaag cctggagatc caggccagcg ccgagcagga ttccgagccg ccgtgggcat
     3181 ttcgcaaaat ccaggtgcac tatgtgctgc atggttctgg tctgaccgat accgcagtca
     3241 gtcaggccat tcagctttcc gaagaaaaat actgctcggt cgcggctacc gtgcgcggag
     3301 tcgccgaagt gcacacctcg tttgaaatcg tctaatttcc ccgtcacccg tccaatgacc
     3361 tgcaagctcc ggcttgcagg tctccatatc ttcagggaga gatcaaatgg ctaaaccagc
     3421 atccaatgcc cccaaattca tgagcctgcg gctcaaactg ctgctgccca tcctggcgct
     3481 gctcaccgtg attttcattg tggcctacgt cggtttgagt gccttcctta acaacactgt
     3541 gtttggcatt ctggacgaag aatccaagac catcatcgat tcggtgaccg cctgcctgga
     3601 tggcga
//
LOCUS       DAOPVS010000181         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_26279_length_3600_cov_55.097884, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000181 DAOPVS010000000
VERSION     DAOPVS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26279_length_3600_cov_55.097884"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1914)
                     /locus_tag="PKL11_10220"
     CDS             complement(7..1914)
                     /locus_tag="PKL11_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNZ14016.1"
                     /translation="MTSRPFTPTKLKRRMPVPSDIEIAQEAELKPITQVAEELGLLPE
                     EVEMYGPHKAKIKLEVLSRLSAVPDGKYIDVTAITPTPLGEGKTTTTVGLSQALGAHL
                     GRRVMTCIRQPSQGPTFGIKGGAAGGGYSQVIPMEDFNLHLTGDIHAITAANNLMAAA
                     IDARILHESGATDEQLFTRLFPLDKNGKRHFTRGMIARMAKLGINRTDPDELTPEERK
                     RLCRLDIDPETITWRRVLDTSDRFLRGVTVGQGPEEQEFARPTGFDITVASEIMAILA
                     LTTDLADMRYRLGQIVIGQSRGGEAINAEDLGVAGAMTVLMRDAIKPNLMQTLEGTPV
                     FVHAGPFANIAHGNSSIIADRIALKLADYVITESGFGADMGMEKFFDIKCRASGLIPN
                     VVVLVATVRALKMHGGGPKVVAGKPLDRAYIEENLDLLRAGIPNLLHHIKIARKYGIP
                     VVVAVNRFGPDTDAELELIRRAAIDEGGAEDAVVCSHWADGGPGALALAEAVVRAAEK
                     PSSFQFLYPLDWSIKRKIETIAREVYGADGVDYSELAEERIADYERLGFGGLPICMAK
                     THLSLSHDATLKGVPTGFRIPVRDIRASVGAGFLYPLLGTMTTMPGLPTRPAFMEVDL
                     DLETGRVLGLF"
     gene            complement(2041..2124)
                     /locus_tag="PKL11_10225"
     tRNA            complement(2041..2124)
                     /locus_tag="PKL11_10225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2088..2090),aa:Leu,seq:taa)
     gene            2256..2603
                     /gene="rplS"
                     /locus_tag="PKL11_10230"
     CDS             2256..2603
                     /gene="rplS"
                     /locus_tag="PKL11_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNZ14017.1"
                     /translation="MEDIIKALEAPVNPNIPHLQTGDSVSVHVKIREGTRERIQEFRG
                     TVLMVHNNGANSTFTVRRVASNGIGVERTFLTRSPRIDKVVVERHNKIRRARLFFLRT
                     RTGKSARLKQKFS"
     gene            2698..3414
                     /locus_tag="PKL11_10235"
     CDS             2698..3414
                     /locus_tag="PKL11_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNZ14018.1"
                     /translation="MHPLIGITTNRVANAKNPFHTVTLSESYLRAVQAAGGTPVLLPS
                     IAPKETWDVLWERLDGILFTGGDDVDPARFNGLPHPRVGGIDEPRDDFEIAFLQRAVK
                     EDKPFLCICRGIQVMNVALGGTLYTDIADQREGAEKHDWYPDWPRDRRSHTVKVMANS
                     RLAGLMQSDEVGVNSLHHQGIWDLAAGLQLTGTAPDGLIEAVEVPGHRFAIGVQWHPE
                     WLQDDAAMRRLFEALMAAAA"
     gene            3419..>3600
                     /locus_tag="PKL11_10240"
     CDS             3419..>3600
                     /locus_tag="PKL11_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNZ14019.1"
                     /translation="MTAAPIGIFDSGVGGLSVVRAIQRILPEHPLIYMADQAHVPYGP
                     RPLEEVRQFSESITRY"
BASE COUNT          748 a          967 c         1130 g          755 t
ORIGIN      
        1 gggaagttag aagaggccca gcacccgacc cgtttccagg tcaaggtcaa cttccataaa
       61 ggccgggcgg gtgggcaggc cgggcatggt ggtcatggtg cccaggaggg gatagaggaa
      121 acctgcccca accgaggcgc gaatatcgcg cacggggata cggaagccgg tggggacgcc
      181 tttgagggtg gcatcgtggc tgaggctgag atgggtcttg gccatgcaaa tgggcaggcc
      241 gccaaagccc aggcgctcat aatcggcaat gcgttcttcg gccagttcgg agtaatcaac
      301 accatcggcg ccatagacct cgcgggcaat ggtttcgatc ttgcgcttga tggaccaatc
      361 cagcgggtag aggaactgga agctggaggg tttttcagcg gcgcgcacca cggcttcagc
      421 caaggccagg gcgccggggc cgccatctgc ccagtgggag cagaccacag cgtcttcggc
      481 gccgccttcg tcaatcgccg cgcggcggat cagctcaagc tcggcatcgg tatcggggcc
      541 aaagcgattg acagccacca ccacgggaat gccatacttg cgggcaattt tgatgtgatg
      601 cagcagattg ggaataccgg cgcgcagcag gtccaggttt tcttcgatgt aagctcgatc
      661 caacggtttg ccagccacaa ccttagggcc gccgccgtgc atcttgaggg cccggacagt
      721 tgctaccaga acaaccacat tggggattaa accggaagcc cggcatttga tatcgaagaa
      781 cttttccatg cccatatcgg cgccaaagcc cgattcggtg atgacataat cggccagttt
      841 gagggcgatg cgatcggcga tgatggagct gttgccgtgg gcaatattgg caaaggggcc
      901 ggcatggaca aagaccgggg taccctccag ggtctgcatc aggttgggtt tgatggcgtc
      961 gcgcatcaaa acggtcatgg cgccggcaac gcccaggtct tcggcattga tggcttcacc
     1021 gccgcggctc tggccaatga caatctggcc caggcggtag cgcatatcgg caaggtcggt
     1081 ggtgagggcc aggatggcca taatctcgct ggcgacggtg atatcaaaac cggtggggcg
     1141 ggcaaattcc tgttcttcag ggccctgccc aaccgtcaca ccgcgcagga agcggtcact
     1201 ggtatcgaga acgcggcgcc aggtgatggt ttcggggtca atatccaggc ggcagaggcg
     1261 tttgcgctct tcgggggtca gttcatccgg gtctgtgcgg ttgatgccca gtttagccat
     1321 gcgggcaatc atcccgcggg tgaagtggcg cttgccgttc ttatcgagcg ggaagagacg
     1381 ggtgaaaagc tgctcatcgg tggcgccgga ttcgtgcaga atgcgggcgt caatggcggc
     1441 agccatcagg ttgttggcgg cggtaatggc gtgaatgtcg ccggtgaggt ggaggttgaa
     1501 gtcttccatg gggatcacct ggctgtaacc gccgccagcc gcgccgcctt tgatgccaaa
     1561 ggtgggcccc tggctgggct ggcggatgca ggtcatcaca cggcggccca ggtgagcgcc
     1621 cagcgcctgg ctgaggccca cggtggtggt ggttttgcct tcacccagcg gcgtgggggt
     1681 gatggcagtg acatcgatat atttgccatc cggcacagcg ctgaggcggg agaggacttc
     1741 cagcttgatt ttggccttat gtgggccgta catctccact tcttcaggca ggaggcccag
     1801 ttcttcagcc acctgggtaa tgggttttaa ttcggcttcc tgtgcaatct caatatctga
     1861 gggaacaggc atgcgccgct tcagttttgt aggggtaaat ggccgggaag tcatgaaatt
     1921 cgctcctttc agcgccagaa attaatcgtc taatctgaac ctgattatgg atgaaaatgg
     1981 gaagaaaatc aagcccgaaa gacaaaggct gcaccggatg gtacagcctc tagtcatctg
     2041 tggggcgggt gggggtcgaa cccacacagc ctctcggccg gcagatttta agtctgctgc
     2101 gtctgcctat tccgccaccg ccccaatagg tcttattgta ctcgttccag aatatccgtc
     2161 aagaccttgt ccgcaatcaa atgccgtgtt ataatctggc ctcgcgctac caaggaacac
     2221 aatattttac aatcgggtaa ggattggtta taaaaatgga agacataatt aaggctctgg
     2281 aagcccccgt aaaccccaat attccgcatc tgcagaccgg cgattcagtc agtgtgcacg
     2341 ttaagattcg cgaaggcacc cgcgaacgta ttcaggagtt ccgcggcacg gtactgatgg
     2401 tgcacaacaa cggcgccaac tcgacattta ccgttcggcg tgtggcctcc aatggcatcg
     2461 gcgtggagcg cacgttcctg acgcgctcac cccggattga caaagttgtg gtggaacgcc
     2521 ataacaaaat ccgccgcgct cgcctattct tcctgcgcac ccgcactggg aagagtgccc
     2581 gcctcaagca gaagttctcg taagagactc tgcgtggatt tgctgactca gcagggtgca
     2641 gccagatggt tgcgcccttt ttccatattc taacctgctg caaactacga ggttcccatg
     2701 caccccttga ttggaatcac aaccaaccgc gttgccaacg ccaagaatcc atttcacacc
     2761 gtgacgctgt ctgaatctta tttgcgggcg gtacaggcgg ccggcggtac gccagtgctg
     2821 ctgccatcca tcgcacccaa agagacctgg gatgttctgt gggaacggct ggatgggatt
     2881 ttatttactg gcggcgatga tgttgacccg gcgcgtttca atgggctgcc ccacccccgc
     2941 gtgggcggca ttgatgagcc gcgcgatgac tttgaaattg cctttttaca gcgtgcggtc
     3001 aaagaagaca agccattttt gtgcatctgc cgggggattc aggtgatgaa tgtggcgctg
     3061 ggtggaacgc tgtacacgga tatagcggat cagcgtgagg gagccgaaaa acacgactgg
     3121 tacccggatt ggccacgcga ccggcggtct catactgtga aggtgatggc taattcccgg
     3181 ctggcgggac tgatgcaaag cgacgaggtt ggcgtgaaca gcctgcacca ccagggcatc
     3241 tgggatctgg ctgcagggct gcaactgacc ggtacagcac cagacggctt gattgaagcg
     3301 gttgaagtgc ccgggcaccg gtttgccatt ggcgttcagt ggcacccgga atggctgcag
     3361 gatgatgcgg caatgcggcg gctctttgag gctttgatgg cggctgctgc ctgagcctat
     3421 gactgccgca ccgattggga tttttgattc gggtgtgggc gggctgtcgg tagtacgcgc
     3481 catccaaagg attctgcctg aacaccccct gatttacatg gctgaccagg cgcacgtgcc
     3541 gtatgggccg cgcccgctgg aagaagtgcg ccagttttct gaaagcatta cccgatattt
//
LOCUS       DAOPVS010000182         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_26630_length_3566_cov_59.201937, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000182 DAOPVS010000000
VERSION     DAOPVS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26630_length_3566_cov_59.201937"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKL11_10245"
     CDS             <1..526
                     /locus_tag="PKL11_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ14020.1"
                     /translation="KALRREMRVHIHAHRADDILTAVRIAEEFNLDVNIEHATEGYKI
                     ASILAEKKIGCTVGPILFSRSKYELRNMTPANPGILARAGVKVAIQTDEMSAVRYLAI
                     NAALAVREGMPEEEAVKAVTINAAEIIGVQDRVGSLEPGKDADVVIFSGHPFDYRTVA
                     EKVFVEGELVYERK"
     gene            complement(584..1399)
                     /locus_tag="PKL11_10250"
     CDS             complement(584..1399)
                     /locus_tag="PKL11_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ14021.1"
                     /translation="MNEITGTLQRQKLFGSRYVNPRTVDVWLPPAYDPAQRHAVLYMH
                     DGQNLFDPTLAYGGVDWGMDETISRMTATGELPPLIVVGVWNTAKRVQEYMPQKPLAG
                     VSARPLLAEFISKAGGAPLSDLYLRFLVEEVKPWVDANYPTITDPSHTAIMGSSMGGL
                     ISLYATVEYPQVFGRAGCVSTHWPIGGELLIEQLCAALPKPGQHKFYFDFGTRTLDAA
                     YEPFQTRWDAEIAAVGYTHNVDWLTLKFEGAEHSETSWRERVHIPLKFLLGQE"
     gene            complement(1392..2300)
                     /locus_tag="PKL11_10255"
     CDS             complement(1392..2300)
                     /locus_tag="PKL11_10255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ14022.1"
                     /translation="MSIKMRPFVGKNVLNAMTALGQTYPEGWIHSADLPYRLSSWALD
                     DPANIGLWEDDQGALLAWAVLNTPFWTMDYKLHPAAPPELYREVLGWLNGRATALAQT
                     PDGHECWFVMMPARESFARQELEAAGFASQADVGEDSWCKVFMARNPTLPVGAYPAPK
                     GFTVRPLKGEGEVAAYVEMHRAVFESKNMTIEWRARTLKRPEYRPDLDIVIEAPDGRL
                     AAFCIGWLTPGGIGQVEPLGCLAEFRRAALGRVALAEVLRRLAAAGATTICVETDTYR
                     NTAFGLYKFMDFEVVDDVIVYRKDYE"
     gene            complement(2395..>3566)
                     /locus_tag="PKL11_10260"
     CDS             complement(2395..>3566)
                     /locus_tag="PKL11_10260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ14023.1"
                     /translation="EPGFSLWLDRMPEGPMLNFNTFAFNPPIQAGIRAAGYSTPTPIQ
                     HQTIPAILDGRDVLGLAQTGTGKTAAFVLPILQRLLSGRPNKARALILAPTRELAEQT
                     HMAIRRLGEKTGLRSVTMYGGVSPQPQIRALRAGAEIIVACPGRLLDLMNQKLVYLGG
                     IEVVVLDEADRMFDMGFLPDIRRIMAALPNQRQTLLFSATMPPEIRKLADEFLHHPVT
                     VEIDTERPVDTIKQTICSVAPDRKYEALMELLPEAGNGQVLIFTRTKHRAKKLSMLLS
                     NAGISTTSLQGNLSQGQRQLAMDGFRSGRVKVMVATDIAARGLDVAKISHVINYDMPD
                     TSDAYTHRIGRTGRMRSVGAAISLVTREDMPMLRAIEKMLGHRLERHPVTEIQNA"
BASE COUNT          741 a         1107 c          971 g          747 t
ORIGIN      
        1 caaggccctg cgtcgtgaga tgcgcgtcca cattcacgcc caccgcgcgg atgatatctt
       61 gacggctgtg cgcattgccg aggagttcaa cctcgatgtc aatattgagc atgccaccga
      121 aggctacaaa atcgccagca tccttgcaga gaagaaaatc ggctgcacgg ttggccccat
      181 cctcttctcc cgctccaagt acgagctgcg caacatgacc cccgccaacc ctggcattct
      241 ggcccgcgcc ggcgtcaagg tcgccatcca gaccgatgag atgagcgccg tccgctacct
      301 ggcgatcaat gccgcgctgg ccgtgcgcga gggcatgccc gaagaagaag ccgtcaaagc
      361 cgtgaccatt aacgccgccg aaattatcgg cgttcaggat cgcgtgggca gcctggaacc
      421 cggcaaagac gccgatgtcg tgatcttcag cggccacccc tttgattacc gcaccgtcgc
      481 tgagaaggtc tttgttgaag gcgagctggt ttacgaacgc aagtaaccgg attttccctt
      541 acccgaaaat cgagggcggc atcagccgcc ctcgtctttt ttactattcc tgtcccagca
      601 aaaactttag cggaatatgc acccgctcgc gccaggatgt ttcagaatgt tcggcgccct
      661 caaacttcaa cgtcagccaa tccacattgt gggtatatcc aactgccgca atttctgcat
      721 cccaccgggt ttgaaacggt tcatacgctg catccagtgt ccgagtgcca aagtcaaaat
      781 agaatttgtg ctgccccggc ttgggcagcg ccgcgcacaa ttgctcaatc agcagttcgc
      841 cgccaatcgg ccaatgcgtt gagacacacc ccgcccgccc aaacacctgt ggatattcca
      901 ctgtcgcgta gagcgagatc agcccgccca tactggagcc cataatcgct gtatgagacg
      961 ggtcagtgat tgttgggtaa ttcgcatcta cccagggctt cacttcttcc accaggaacc
     1021 ggagatatag atctgagagc ggcgccccac cggctttgct gatgaactct gccagcagcg
     1081 gccgcgccga aacgcccgcc agcggctttt gcggcatgta ttcctgtacg cgtttggctg
     1141 tattccacac gcccaccaca atcaggggcg gcagctctcc ggtggcagtc atccggctga
     1201 tggtttcgtc catgccccag tccacgccgc cataagccag tgtggggtca aagaggtttt
     1261 gcccatcatg catgtacagg actgcatggc gttgcgccgg gtcataggct ggcggcagcc
     1321 atacgtccac tgttcgtggg ttgacgtacc gcgagccaaa caatttttga cgttggagtg
     1381 tacctgttat ctcattcata gtcttttcga tagacaatca catcatccac cacttcaaaa
     1441 tccatgaatt tatacaatcc aaaggctgtg ttacggtagg tatcggtctc aacgcagatg
     1501 gtcgtggctc ctgcggctgc cagccggcgc agcacctcag ccagcgccac ccgtcccagc
     1561 gcagcccggc gaaactctgc caggcatcct agcggctcca cctgcccaat accgcccggc
     1621 gtcagccagc caatgcagaa cgccgccagc cgcccatccg gcgcttcaat gacgatatcc
     1681 agatctgggc gatattctgg ccgttttagc gtccgcgccc gccactcgat agtcatgttc
     1741 ttgctttcaa acacagcgcg gtgcatttcc acataggctg ccacctcgcc ctcacctttc
     1801 agtggacgca cggtgaagcc cttgggcgcc gggtaggctc ccactggcag cgtcgggttg
     1861 cgggccataa acaccttgca ccaggaatct tcccccacat ccgcctgcga ggcaaatccg
     1921 gcggcttcca gttcctgtct ggcaaaactt tctcgggcag gcatcattac aaaccaacat
     1981 tcgtgcccgt ctggggtttg ggccagcgct gttgcacgcc cgttcaacca gcccagcacc
     2041 tcccggtaaa gctcaggcgg tgctgccggg tgcagcttgt aatccatcgt ccagaacggc
     2101 gtattcagca ctgcccaggc cagcagtgcg ccctggtcat cttcccacag cccaatgttc
     2161 gcaggatcat ccagcgccca ggaactgagc cggtagggca aatctgcact gtgaatccag
     2221 ccctccgggt aggtttgccc cagggcggtc atcgcgttca ggacattctt gccaacaaat
     2281 ggacgcattt tgatggacat ctgaacctcg ttctgccggc tgaaataaaa aaaggaagtc
     2341 gagggtttac cctcgacttc catggtagta caggtcaaat gaaacgtcaa gccatcaagc
     2401 gttttggatt tcagtaaccg ggtgccgctc caggcggtgc cccagcatct tttcgatcgc
     2461 acgcagcatg ggcatatcct cgcgcgtgac cagagaaatc gcggcgccga cgctgcgcat
     2521 gcgcccggtg cggccgatgc ggtgcgtata ggcatcggat gtgtccggca tgtcataatt
     2581 gatcacatgc gaaatcttcg ccacatccag cccgcgggca gcaatgtctg tggctaccat
     2641 caccttcacc cgcccgctgc ggaagccatc catcgccagt tggcgctgcc cctgcgacag
     2701 gttcccctgc agcgaggtgg tggatatgcc agcgttggaa agcagcatac tgagcttctt
     2761 ggcgcgatgc ttggtgcggg taaaaatcag cacctgtcca ttgccagctt cgggcagcag
     2821 ttccatcagc gcttcatact tacggtccgg tgcaacgctg cagatggtct gcttgatggt
     2881 atccaccggg cgttcggtat caatttctac ggtaacggga tgatgtaaga attcatccgc
     2941 cagcttgcga atttccggcg gcatggtggc cgagaatagc agtgtttgcc gttgattagg
     3001 cagagccgcc ataatccggc gaatatcggg caaaaagccc atatcgaaca ttcggtcagc
     3061 ttcatccagc accacgactt cgatgccgcc cagatagacc agtttctgat tcatcaggtc
     3121 cagcaggcgt ccggggcagg ccacaatgat ctcggctccg gcgcgcagcg cccggatttg
     3181 gggctgcggg ctgacgccgc catacatcgt cacactgcgc agtccggtct tttcgcccag
     3241 gcgtcggatt gccatgtggg tttgttcggc cagctcgcgg gtaggtgcca ggatcagcgc
     3301 ccgcgccttg ttggggcgtc cgctcagcag gcgctgcaaa atcggcagca caaacgccgc
     3361 ggttttgccg gtgccggtct gggccaggcc cagtacatcg cgcccgtcta aaatcgccgg
     3421 aatggtctga tgttgaatgg gggtcggcgt ggaatatcca gccgctcgaa tacccgcctg
     3481 gatgggcggg ttgaaagcaa acgtattgaa atttagcata ggtccttctg gcatacgatc
     3541 gagccagaga gaaaacccag gctcaa
//
LOCUS       DAOPVS010000183         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_27111_length_3521_cov_60.928159, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000183 DAOPVS010000000
VERSION     DAOPVS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27111_length_3521_cov_60.928159"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKL11_10265"
     rRNA            complement(<1..327)
                     /locus_tag="PKL11_10265"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(906..1970)
                     /locus_tag="PKL11_10270"
     CDS             complement(906..1970)
                     /locus_tag="PKL11_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14024.1"
                     /translation="MKKQVVLLLFALLVLSACTPTPAVPMTSAIPTPTAALPSPTPTT
                     PTPTLAYESPLMPITAENVNRLVAVEVPLPALAQDLHWGAFNQLLALCQPNAENNAAL
                     AAYALFPPAPTTSAEYPASLTLPPNLLAVPRHPIELALNNGNSVVTTSLDGAPLRTLE
                     VGGPVYGAAYAPDQQTLVIYLADRWEAQVWSLSTGERLTTLTGFETAAPVYTIFPGPN
                     EIAWVARGTLQLQNIQTGALAPAAHYSDFITAVSFAPLEDGALGVLVAGQLHLIRPDT
                     ATETLVLEAPGALALAFSPDGQIVAAGGSQGVSLWDVNTGALLASLPGSLTRLSFSDD
                     GTTLVTIQNDRLALLLWQPR"
     gene            complement(2091..>3521)
                     /locus_tag="PKL11_10275"
     CDS             complement(2091..>3521)
                     /locus_tag="PKL11_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNZ14025.1"
                     /translation="VVVAGYEAAYNTSTPSIWAHRWSLNDVTPPTLDGKILGEYSHNG
                     GYAQFGEIHGDHPATIGIMAHELGHDITWPDLYDTDGSSDGVGYWSIMGSGNWNYTDS
                     TRPGNSPALPDAWLKWYQGWITPTTVSGTLTNQAIPQAETNPTAFLLRPNSGGVNWEF
                     GQYSGTGEFFLVENRQLTGYDAGLPGCGLLIWHIDESVTYTNSANDNEYHPLVKLMQA
                     DGLDQLMDGGGERGDPGDPFPGSANNTAFTYSSVPNSRLYSGSDSLASVTAISAGCAA
                     TKTATLTYGGTTAASGIFLPVIVKMQPPAPVNPIRNGTFEAGVDGNWSQYSSGGWPLI
                     TQTLPGGVAPHGGSWAVWMGGAPSETAQLTQSGISMTGARYLHYWYWIASEDMCGYDY
                     AGVFVNGSLLKQYDLCSSSSTGGWVQQLLDLNAYAGTTITLTFAVTTDGSFNSNFLLD
                     DVSIGATTALQPMEPQMMFEGASAPR"
BASE COUNT          731 a          938 c         1078 g          774 t
ORIGIN      
        1 accgtgtttc agttccattg tggggggcca ccctctcagg tcccctaccc gtcatagcct
       61 tggtaggcca ttaccctacc aactagctga tgggacgcag gcccctcccg aagcgacttt
      121 cgcctttaat caccagattc taaaaccggg gatcacatgg ggtattagca gtcctttcgg
      181 actgttatcc ccctcttcgg ggcaggtcac caacgcgtta ctcacccgtt cgccactaag
      241 acagtattgc tacagtctcc gttcgacttg catgtattaa gcacgccgcc agcgttcatc
      301 ctgagccagg atcaaactct ccgctaaatt tttcaccttt tgcaaggtgt ttcttacgga
      361 tttatttatt gaacgacaga gctgttgtct tcctatcact cttcagttgt taaggtcctg
      421 cttgacttcc gagggctgga ttctaaccgc ttgtttgcgg tctgtcaagg gccaatttcg
      481 tccggtttta cccgacaaaa ttgccgatgt atctcctgtc taactgggtc aacaccggct
      541 gcttcagcgc tcgcctttga cttgaacggt cagcgtccct gctcacttgt tatcggtctg
      601 cctttttaca ggccaaaccg acctcatttg actgagggac ttttattgta ccgctcacat
      661 ctacccttgt caagggtttt ggtcgatttg attttgatca attttatcga ccgggcccct
      721 gttgggaagc tgccttttgg gctgtttggg ttttcaatga tctgctgtct atttcagcga
      781 gatcttgttg ttaccttctt gctggcaacg ggctgaatta tacgcaaacg aaaagttctg
      841 tcaaggggtt ttaagaccaa ttttgaaaat tggtctgaca actttggaaa ttggtgccat
      901 ttggttcagc gcggctgcca cagcagcaag gccagtcggt cattctggat ggtgaccagg
      961 gtggtgccgt catctgagaa gctgaggcgg gtgaggctgc ccggcaggga ggccagcagc
     1021 gcaccggtgt tcacatccca cagggagacg ccctgactgc ccccagcagc cacaatctgc
     1081 ccatcgggag aaaaggccag tgctagagcg ccgggggcct ccagcacaag cgtttctgtc
     1141 gcggtatcag gacgaatcag gtgaagctgc ccggcaacca gaacgcccag agcgccgtcc
     1201 tccaggggag caaaggacac tgcggtgata aagtcggaat agtgcgcggc aggggccagg
     1261 gcgccggtct ggatgttttg gagctgcagg gtgccccggg ctacccaggc aatctcattg
     1321 gggccgggga agatggtgta gaccggagcg gcggtctcga agccggtcag ggtggtcagg
     1381 cgctcgccgg tggacaggga ccagacctgg gcttcccagc ggtcagccag gtagatgacc
     1441 agggtttgct gatccggggc gtaggcggcg ccgtaaaccg gcccgcccac ctccagtgtg
     1501 cgcagagggg cgccatccag gctggtggtg actacagaat tgccgttgtt cagcgccagc
     1561 tcaatggggt ggcgcgggac agccagcagg ttgggcggca gcgtgaggga cgcggggtat
     1621 tcggcgctgg tggtgggcgc ggggggaaac agggcgtaag cagccagggc ggcattgttc
     1681 tcggcattgg gctggcagag cgctaagagc tggttgaagg cgccccaatg caggtcctgg
     1741 gccaatgccg ggagcggaac ttcgactgca accaggcggt taacgttttc ggcggtgatg
     1801 ggcatcaatg gggattcgta ggccagtgtg ggagttggcg tggtgggtgt gggagacggc
     1861 aatgcagccg tgggagtggg tatcgccgag gtcattggca cagccggtgt gggtgtgcag
     1921 gcagacaaaa ccagcagggc aaagagcagc agaacaactt gttttttcat cacagcctcc
     1981 tggagcgcgt ggtgttcctt cattaagacg cacgccgcgc cattgagttc ccctggggcg
     2041 aaaaaagggg agggcgactt gcgccgccct cctgcaggtt gaaaatcatt tcagcgcggg
     2101 gcgctggcgc cctcaaacat catctgaggt tccataggct gcaaagccgt ggtcgcccca
     2161 atggagacat catccagtaa gaagttgctg ttgaacgagc catctgtagt gactgcaaat
     2221 gtcagggtaa tggttgttcc ggcgtaggca tttaagtcca gtaattgctg cacccagccg
     2281 ccagtgctgc tactggagca aagatcatac tgcttgagca aggagccgtt cacaaaaacg
     2341 ccggcataat catagccaca catatcttcc gaggcgatcc agtaccagta gtgcaagtac
     2401 cgcgcgcccg tcatggaaat tccagattgg gttaactggg ctgtttcgct tggagcaccg
     2461 cccatccaaa ccgcccagga gccgccatgc ggggccacac cacctgggag ggtctgggtg
     2521 atcaaaggcc agccaccgga ggagtattga gaccagttgc catctacgcc agcctcaaag
     2581 gtgccattgc gaatgggatt gaccggggcg gggggctgca ttttgacgat gaccgggagg
     2641 aaaatgccgg atgcggcagt tgtaccgcca tatgtcagtg tggcggtttt ggtggcggca
     2701 cagccagcac tgatggcggt gacggatgcc aggctatcgg agccagagta taaccggctg
     2761 tttgggacgg agctgtaggt aaaagctgtg ttgttggcag accctgggaa gggatcaccg
     2821 ggatcacccc gctcacctcc cccatccata agttgatcca ggccatccgc ttgcatcagt
     2881 ttcaccaggg gatgatactc gttatcattg gcagaatttg tataggtgac cgattcgtca
     2941 atatgccaga tgagcaaacc gcagccaggt aggccggcat catagccggt gagctggcgg
     3001 ttttcaacca ggaagaactc gcctgtgccc gaatactggc caaattccca gtttacgccg
     3061 cccgaattgg ggcgcagcaa gaaggccgtg ggattggttt cggcctgcgg aatggcctgg
     3121 ttggtcaggg tgccgctgac cgtggtcggg gtaatccagc cctgatacca cttgagccag
     3181 gcatctggca gcgccgggga attgcctgga cgcgttgaat ccgtataatt ccagttaccc
     3241 gagcccataa ttgaccagta ccccacgcca tccgaagacc cgtcggtgtc gtataaatcg
     3301 ggccaggtga tatcgtgccc aagctcgtgg gccataatcc caatagtggc gggatggtcg
     3361 ccgtgaattt cgccaaactg ggcataaccg ccattatgtg agtactcgcc caggatcttg
     3421 ccatccaggg ttggcggggt gacatcattc aaactccagc ggtgcgccca gatgctaggg
     3481 gttgaggtgt tgtaggcggc ctcgtaaccg gcaacaacaa c
//
LOCUS       DAOPVS010000184         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_27598_length_3476_cov_33.478515, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000184 DAOPVS010000000
VERSION     DAOPVS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27598_length_3476_cov_33.478515"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..764)
                     /gene="rpe"
                     /locus_tag="PKL11_10280"
     CDS             complement(102..764)
                     /gene="rpe"
                     /locus_tag="PKL11_10280"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNZ14026.1"
                     /translation="MTELLISASILSADFGALGEAIRQAEAAGVDWIHIDVMDGHFVP
                     NITVGPVIVEACRKITHLPLDVHLMIEQPERHIEAFARAGANILTVHIENAPNIHRTL
                     QQIRALGCQTGIAINPGTTAASIEAVAPLLDMALVMSVNPGFSGQAFLPEVVEKVSAV
                     RSLLDQRCPTARVQVDGGITAETLPVMKTAGASVFVAATAIFRHPDGIAAGIRALRSA
                     AP"
     gene            complement(770..1855)
                     /locus_tag="PKL11_10285"
     CDS             complement(770..1855)
                     /locus_tag="PKL11_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNZ14027.1"
                     /translation="MQAQPAASKPILSNRYQLLQAIGTGGMAVVYRARDMLLERPVAI
                     KVLRENYSRSEDFRERFKQEAQAAANLSHPNIVTVHDFGLDQGRLFLVMEYIGGTDLK
                     AIIKQRGRLELDEAVGLMVQACAGIGYAHRAGIVHCDVKPHNMLVTPDQRLKVADFGI
                     ARALSSIKPDEQHDVIWGSPLYFSPEQAAGLPPMPASDVYSLGLVLYEMLTGQLPFQS
                     EDPVELARMHREVLPPHPRRLNPDIPPTLEQILLKVLSKEPTARYRTADQFGRVLQTF
                     GTQPEPIEARTPPAATRPATPQPQPEPTIIEPAPTTPEDTQPQRPETAPVSIDWLAVF
                     LGLLAVLAVGGLVPFWLYIWFSFNPVP"
     gene            1957..3048
                     /gene="cax"
                     /locus_tag="PKL11_10290"
     CDS             1957..3048
                     /gene="cax"
                     /locus_tag="PKL11_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HNZ14028.1"
                     /translation="MKKVWAYIKKNPLSLLLIALPLALLAELLHWGALWVFIFSALGV
                     VPMASYIGSATEALAHYTGPKIGGLLNATLGNAAELIITLVAIKAGLLDLVKASITGS
                     IIGNLLLVMGLAMVIGGARHGLQTFDRRQSTNHAILLTLAILALAIPSFFNHFIAEYS
                     PEVEGLSLGVSVVMILLYGLGILYSLKFNNSPMTHTPPVDHGKNHVKPEYTIWQAILV
                     LALATAGVVWLSELLVGAVEVVVADLGLSEFFLGIILIPIIGNVAEHLVAVQVAARNH
                     MDLSVEIAVSSSLQIALFVAPLLVFISLMMGNPLTLVFNPFELVALGAAVVITALVCF
                     DGESNWLEGAALLSIYLILGLAFFMMPVG"
     gene            3071..>3476
                     /locus_tag="PKL11_10295"
     CDS             3071..>3476
                     /locus_tag="PKL11_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14029.1"
                     /translation="MTPWVIGGALLAALLFCLAAALLLGLFRRPAAPETQPTAELTVL
                     AAPTATSVLPSIFDTPTPTPTPVEVGGISIGTYVQISGTDGAGLRLRSGAGTSYEQLF
                     LGYDSEVFKVIDGPIVNDGYTWWRLEAPYDTTR"
BASE COUNT          725 a          980 c          938 g          833 t
ORIGIN      
        1 aggtgctgtg cgccaagtgc atccgctcga tggcgaagtc ggcctaaggc taccgattcc
       61 ctgagaacac aagaatcgcg gcatttgccg tgatttttgt tttaaggtgc tgccgagcgc
      121 agggctcgga ttccggcggc aatgccatcc gggtgtctaa aaatagccgt tgccgccaca
      181 aacacactgg cgccggctgt cttcataacg ggtaatgttt ctgcagtgat gccgccatca
      241 acctgcaccc ttgctgtggg gcagcgttga tctaaaagcg accgtaccgc gctcactttt
      301 tcaaccactt cgggtaaaaa ggcctgtccc gaaaacccgg ggttcacgct catcaccagc
      361 gccatgtcta acagcggcgc gaccgcctca atgctcgcag cagttgttcc ggggttaatg
      421 gcaatgcctg tctggcagcc caaggctcga atttgctgca gagtgcggtg aatattgggc
      481 gcattttcaa tgtgaaccgt gagaatattg gctccagcgc gtgcaaaggc ctcaatgtgc
      541 cgctcgggtt gctcaatcat caggtgaaca tccagtggta agtgggtgat cttccggcag
      601 gcctcaacaa tcaccggccc aactgtaata ttggggacaa aatgaccatc catgacatca
      661 atatggatcc agtccacacc ggcagcttca gcctggcgaa tggcttcgcc cagggcgccg
      721 aaatcggcag ataaaatcga ggcagaaatc aaaagctctg tcatgggctt tatggcaccg
      781 ggttaaaact gaaccagata tagagccaga acggcaccag cccgccaacc gccagaaccg
      841 ccagcagtcc caggaacaca gccagccaat caatgcttac gggcgctgtt tcgggccgct
      901 gcggctgggt atcttcgggt gtggttggcg ccggctcaat aattgtgggc tcaggttgtg
      961 gctgtggggt tgccggtcgg gtggcagccg gtggggtgcg ggcttcaatc ggctctggct
     1021 gagtgccaaa ggtctgcagc acccgcccaa actggtcagc cgtgcgatat cgcgccgtcg
     1081 gctcttttga aagcaccttg agcagaatct gttccagcgt tggcggaata tcggggttca
     1141 gccgtcgggg gtgggggggc agcacttcgc ggtgcatccg cgccagttcc accggatctt
     1201 ctgattggaa cggtagttgc ccggtcagca tctcatataa caccaacccc agcgaataaa
     1261 catctgaagc cggcattggt ggcagcccgg cagcctgttc gggtgaaaaa tacagtggcg
     1321 agccccaaat tacatcatgt tgttcgtctg gcttaatgga agacagagcg cgcgcaatgc
     1381 caaaatcagc tactttaagt cgctggtcag gtgtaaccag catattatgc ggcttcacgt
     1441 cgcagtgaac aatcccagcc ctgtgcgcat agccaatacc ggcgcaggcc tggaccatca
     1501 atccaaccgc ctcatcaagc tccagtcgcc cgcgctgttt gatgatcgcc ttgaggtctg
     1561 ttccgccaat atattccatt accagaaaca gccgcccctg gtccaatcca aaatcgtgca
     1621 cagtgacaat attggggtgg gagaggttgg ccgcggcctg ggcttcctgc ttaaatcgct
     1681 ctctaaaatc ctcactgcgt gagtaattct cgcgcagcac cttgatcgcc accgggcgct
     1741 ccaacagcat atctctggcg cggtaaacaa ctgccatgcc gccagttcca atggcttgca
     1801 agagctggta acgattgctt aaaataggct tgctggctgc gggttgtgcc tgcataatct
     1861 ctcttccgcg attatatcat acgatataat aagcattgat gtctgatggc tgtggattga
     1921 ctcccgcttt tatcctatat tcaggagcat aagaaaatga agaaggtttg ggcttatatt
     1981 aagaaaaacc ctttgagcct cttgctgatt gcgctgccac tggccttgct ggctgaattg
     2041 ctccactggg gagcactttg ggtcttcatt ttttcagcgt tgggcgttgt gcccatggct
     2101 agttatattg gctcggctac cgaagctctg gcgcactata ccggccccaa gatcggcggc
     2161 ttgctgaacg ctaccctggg gaacgctgcc gagttgatca tcactctggt agcaattaaa
     2221 gcaggccttc tcgatctggt caaagcttca atcacgggtt ccattattgg caaccttttg
     2281 ctggttatgg gcttggctat ggtcattggc ggcgcccggc atggcttgca aacctttgac
     2341 cgccgccaat ccaccaatca tgccatcttg cttacgctgg caatcctggc gctggcaatc
     2401 ccatcatttt tcaaccattt catcgcagaa tattctcccg aggtcgaggg gctcagcctg
     2461 ggtgtttctg tggtgatgat tttgctttat ggcctgggca tactgtacag ccttaaattc
     2521 aacaacagcc ccatgacgca taccccacct gtagatcatg gcaaaaatca tgtcaaacct
     2581 gaatacacaa tctggcaggc catcctggtg ctagctctgg ctactgccgg tgtcgtctgg
     2641 ctttcggaac tgctggtcgg ggcagttgaa gtggttgttg ctgacctggg gttgtcagaa
     2701 ttcttcctgg gcatcatcct gatccccatt attggcaatg tggccgaaca tcttgttgca
     2761 gttcaggtag ccgcccgcaa tcacatggac ctgagcgttg aaattgccgt atcctccagc
     2821 ctgcaaattg ccttgtttgt ggcgccgctg ctggtcttca tcagcctgat gatgggcaat
     2881 cccctcacac tggtattcaa tccatttgaa ctggttgcgt tgggcgcagc ggttgtgatt
     2941 acagctctgg tctgctttga tggcgaatca aactggctgg agggcgcggc cctgctgtcc
     3001 atttatctga tcctgggatt ggcattcttc atgatgccgg taggataacg tggaggccat
     3061 caagtcccgg ttgaccccct gggtaattgg cggcgccctg ctggcggcgc tgctgttttg
     3121 cctggctgcc gcccttctgt tgggcctgtt ccgccggcca gccgcgccag aaactcaacc
     3181 cactgccgaa ctgaccgtgc ttgctgcgcc aacagccacc tctgtcctgc catccatttt
     3241 tgataccccc acaccaacac ccaccccggt tgaagttggc ggcatatcca tcggcacata
     3301 tgtccagatc agcggtacag atggtgcggg cctgcgcctg cgctcgggcg caggcaccag
     3361 ttatgaacag cttttcctgg gctatgattc tgaagtcttc aaagtcatcg acggccccat
     3421 cgtcaacgat ggctatacct ggtggcggct ggaagccccc tacgacacaa cccgca
//
LOCUS       DAOPVS010000185         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_28170_length_3423_cov_52.452197, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000185 DAOPVS010000000
VERSION     DAOPVS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28170_length_3423_cov_52.452197"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKL11_10300"
     CDS             <1..329
                     /locus_tag="PKL11_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNZ14030.1"
                     /translation="ALRVPTPTVSIVDFVANVERPTTKEELNAAFIEASEGALKGILG
                     VTRGEYGDPLVSSDFKGDHRSSIVDLPNTMVMGGNLVKVVTWYDNEWGYSCRTADLAK
                     LIGGKL"
     gene            357..1532
                     /locus_tag="PKL11_10305"
     CDS             357..1532
                     /locus_tag="PKL11_10305"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNZ14031.1"
                     /translation="MFSKKTIRDIDVKGKRVLVRVDFNVPLKDGVVGDDTRVRAALPT
                     IQYLLEQGAAVILASHLGRPKGGPDPKYTLKPVAEHLAGLLGKPVAFAEDCVGPAAEA
                     AAKALKPGQVLVLENTRFHPEEEKNDLELARQMASLADLYVNDAFGTAHRAHASTEGV
                     THYLPGVAGFLLEKEIQYLGQAVAEPKHPFVAILGGAKISDKIGVIRNLLGKADAVLI
                     GGGMANTFLKAQGYDVADSLVEETAVETAKELLALGAGKLHLPVDLVLADAFDAAAAH
                     KTIPLGNTPAGWRILDIGPETVKAYAAIIAGAGTVVWNGPMGVFEFPVFAEGTYGIAR
                     AVAGSQAVSIIGGGESVAAIQQSGLADKITHISTGGGASLEMLEGLVLPGVAALQDK"
     gene            1644..2459
                     /locus_tag="PKL11_10310"
     CDS             1644..2459
                     /locus_tag="PKL11_10310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNZ14032.1"
                     /translation="MKINWKLWAILAVANLIASIILLPYALALQGDAASLTGGMMPEW
                     AFNLFMLIAQAAQAVLLNAPLILLGLFLADRVGFGVPILSAWLRRESAREPARRMIKP
                     VVIIGLAGGVLLVLLSYGMSALTEAEALRLGYTIPDVALPAPWKGFLASISAGITEES
                     LLRLFLMTFLVWLGTQIWRSPDGRPQAALIWGANILAALLFGALHFMNVTMIGLPFTP
                     MIILQILLLNGSIGVAFGWLYWKYGLESAMLAHFLVDVVLHVLVPLFAPALGV"
     gene            2535..>3423
                     /locus_tag="PKL11_10315"
     CDS             2535..>3423
                     /locus_tag="PKL11_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ14033.1"
                     /translation="MSLDIEVEQLEQAIRALEAQRAVLGDAILDTTLAPMLARLAALK
                     SRVHTGAGQQRKLITVLVTDLSGFTALGEQMDAEDLSALMNNLWQRIDALLTARGGWI
                     YKHLGDAVIAIWGLETTSENDAELAVQSALDIRTEVSRLSPRAGSELPLRLHTGIGTG
                     LALIGTLGSTSERSITGEPLNDANQFVHLAASDEIVISRDTYLHVRGLYDVQPVEVEA
                     SASPCYRVLSARQRAFRLPRRGVEGVEVHTIGRASELRQLQAAWERIIGGGTASVFLL
                     SGDAGVGKTRLLYDFLNWLD"
BASE COUNT          632 a         1093 c          964 g          734 t
ORIGIN      
        1 acgccctgcg cgtccccacc cccaccgtct cgattgtgga ctttgttgcc aacgtagaac
       61 gccctaccac caaagaagaa ctcaatgcgg ccttcatcga agcctccgaa ggcgccctta
      121 agggcatcct gggcgtcacc cgcggcgaat acggcgatcc gctggtttcc tcagacttca
      181 agggcgatca ccgctcctcg attgttgacc tgcccaacac catggtcatg ggcggcaatc
      241 tggtcaaagt ggtcacctgg tacgataacg aatggggtta ctcctgccgc actgccgatc
      301 tggccaaact catcggcggc aaactctaat cccggtccgc gaatctggag cacaccatgt
      361 ttagtaagaa aaccattcgc gatattgatg tcaagggcaa gcgcgtcctg gtgcgtgtgg
      421 acttcaacgt cccgctcaag gatggcgtgg tgggtgatga tacccgcgtc cgcgcagccc
      481 tgcccaccat ccagtacctg ctggaacagg gcgcggcggt catcctggcc tcgcatctcg
      541 gtcgccccaa aggcgggccc gaccccaaat acaccctcaa acccgtggcc gaacacctgg
      601 ctggcctgct gggcaaaccc gtagcctttg cggaagactg cgttggcccc gctgccgaag
      661 cagccgccaa ggccctcaaa cccgggcagg tgctggtgct tgaaaacacc cgcttccacc
      721 cggaagaaga gaaaaacgac ctcgaactgg cccgtcagat ggccagcctg gctgacctct
      781 atgtgaacga tgcctttggc accgcacacc gcgcccatgc ctccaccgag ggcgtcaccc
      841 actacctgcc cggtgtggct ggcttcctgc tggaaaaaga aatccagtac ctcggtcagg
      901 ctgtagccga acccaaacac ccgtttgtgg caatcctggg cggcgccaaa atcagcgaca
      961 aaatcggcgt catccgcaac ctgctcggca aagccgatgc cgttctgatc ggcggcggca
     1021 tggccaacac cttcctcaag gcgcagggct atgatgtggc cgactcgctg gtcgaagaaa
     1081 ccgcggtcga aaccgccaaa gaactactgg cgctgggtgc gggcaagctg catttgccgg
     1141 tggatctcgt cctggccgat gcctttgatg ccgccgcggc ccacaagacc atccccctgg
     1201 gcaacacccc cgcgggctgg cgcatcctcg atattggccc cgaaaccgtc aaagcctacg
     1261 ctgccatcat cgctggcgct ggcactgtcg tctggaacgg cccgatgggc gtctttgaat
     1321 tcccggtctt tgccgagggc acctacggca tcgccagggc cgttgctggc agtcaggctg
     1381 tcagcatcat cggcggcggc gagtcggtgg ctgccattca gcagtctggc ctggctgata
     1441 aaatcacgca catctccacg ggcggcggcg cctccctgga aatgctggaa ggcctggtcc
     1501 tccccggcgt tgcagccctg caggacaaat agcactaccc ctcaatctca aaagacctgg
     1561 cacgggttgc caggtctttt tgatttagat ccgcttaacg cgcttaagtt atgttaaact
     1621 tgtcggtaat tcaggagaac ctcatgaaaa ttaactggaa attatgggcc attcttgcgg
     1681 ttgccaacct catcgcctca ataatattgc tgccctatgc cctggccctg cagggtgatg
     1741 ccgccagttt gaccggcggt atgatgccag aatgggcttt caacctgttc atgttgatcg
     1801 ctcaggctgc gcaggctgtt ttgctcaacg ccccgttgat ccttctgggc ctgttcctgg
     1861 cggaccgggt ggggtttggt gttcccattc tgtcggcctg gctccggcgt gagtcggccc
     1921 gtgagcccgc ccggcggatg attaagcccg tggtcatcat tggcctggct gggggtgtgc
     1981 tgctggtgct gctcagctat ggaatgtcag ccctcactga ggccgaagcg ctccggctgg
     2041 gctacaccat ccccgatgtg gctctgcccg ctccctggaa gggctttctg gcctccatct
     2101 ctgccggcat taccgaagaa tcgttgctgc gcctcttctt gatgaccttc ctcgtctggc
     2161 tgggcactca gatttggcgc tcacccgacg ggcggccaca agccgccctc atctggggcg
     2221 ccaatattct ggctgcgctg ctgttcggcg ccttgcattt tatgaacgtc accatgatcg
     2281 gcctgccctt cacgcccatg attatcttgc agatcttact gttgaacggt tctatcggcg
     2341 ttgcctttgg ctggctctac tggaaatatg gcctcgaaag cgccatgctg gcccacttcc
     2401 tggtcgacgt tgttctgcac gtcctggtcc cgttgtttgc cccggccctg ggtgtttgat
     2461 tgtgccatca aaatttagta acgcatttcg ataaatcggt ataattagtt tatcttcctg
     2521 accaaccggt gattatgtcg ctggatattg aagttgaaca gttggaacag gccatccgcg
     2581 cccttgaggc tcaacgggcg gtcttgggcg acgccattct ggacaccacc ctggcgccca
     2641 tgctggcgcg cctggctgcg ctgaaatccc gggtgcacac aggcgccggg cagcagcgca
     2701 agctgattac cgttctcgtc accgacctct cggggttcac ggccctgggt gaacaaatgg
     2761 acgctgaaga cctcagcgcc ctgatgaaca atctctggca gcggattgac gccctgctca
     2821 ccgcccgcgg cggctggatt tacaaacacc ttggcgatgc cgtaattgcc atctggggcc
     2881 tggaaaccac cagcgaaaat gatgctgaac tggcagtcca gtcagccctc gacattcgca
     2941 cagaagtcag ccgcctctcg ccgcgcgccg gctctgaact gccgctgcgc ctgcacaccg
     3001 gcattggtac cgggctggcg ctcatcggca ccctggggtc taccagcgag cgcagcatca
     3061 ccggcgagcc gttgaatgat gctaaccaat tcgtccactt ggccgccagc gatgagatcg
     3121 tcatctcgcg tgatacctat ctgcatgtac gcggtctgta tgatgtccag ccggttgaag
     3181 ttgaggcttc tgcctcaccc tgctaccgcg tgttgtctgc ccgccaacgt gccttccggc
     3241 tgccgcggcg cggtgttgaa ggagtcgaag ttcacaccat tggccgcgcc tctgaactgc
     3301 gccagcttca ggccgcctgg gagcgcatta ttggcggcgg aaccgccagc gttttcctgc
     3361 tttcgggaga tgccggagtc ggtaaaaccc gcctgctgta cgattttttg aactggctgg
     3421 acc
//
LOCUS       DAOPVS010000186         3399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_28416_length_3399_cov_33.064593, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000186 DAOPVS010000000
VERSION     DAOPVS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28416_length_3399_cov_33.064593"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKL11_10320"
     CDS             complement(<1..475)
                     /locus_tag="PKL11_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ14034.1"
                     /translation="MQIWRVDLQNQTLEQSQVPPEWEHLGGRGLSARILLDEIDPTCD
                     PLSPGNVLVFAPGLLVGHMLSSCDRISIGGKSPLTQGIKESNAGGTTGLHLARLGIKA
                     LILKNHAQSGWRILHLSTQGVRFDHADALVSMGAYQSAQWLIERYGTRNAMALIGP"
     gene            complement(479..2065)
                     /locus_tag="PKL11_10325"
     CDS             complement(479..2065)
                     /locus_tag="PKL11_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNZ14035.1"
                     /translation="MSETAAAIKQETRKLKRELTLLPLFALIYFTVCGGTFGIESLIG
                     WSGPGMALILIAVTPIVFSIPNMLMVREMNSMMPVEGGYYHWVKQAYGPFAGFMAGWM
                     NWVVSWVDVSIYPVLAAYYLGQMIPGLREGTQIAGIWVDGWVLQWLVALVIIWGISAL
                     NIRGARLTGLTANWIGIAMLVPLVLTTIVGIYNWIHGGVTISLPLLPDGQSGFSGITS
                     SLSVGLFVVMWNYMGWELPTAAGDEIVNPKRTYPLAMTLVLVAAILTYSLPVVAGLYG
                     GAGNDGRYLLWGQEEYEEGQGIGPVMAEYGVTDEKLTEWEVNPQSSVGWQFPDIAYRL
                     GEIASGVKNSPLARAMGSSVAFAAVLSMVGLFIGNSLGGTRVPFAMAEDGMMPKWMVK
                     VHPKYGTPWVAIVFCGVLFSIFSLQTFQLLVVVDVLLNMLVLLAQLFALWKLRSSHPH
                     VHRQKVPGGTLGLVLVTLGPTIFAVFAIYSQFAEEGITSISWALVAMLVGAALYFPIK
                     RYIKPGVPDVDPFVSEEETV"
     gene            complement(2130..>3399)
                     /gene="gcvPB"
                     /locus_tag="PKL11_10330"
     CDS             complement(2130..>3399)
                     /gene="gcvPB"
                     /locus_tag="PKL11_10330"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNZ14036.1"
                     /translation="YPLGSCTMKYNPKINEDTARLSGFAYTHPLQPIETVQGNLALMY
                     ELQQYLQEISGFAGCTLQPAAGAHGEFVGVMIIRAYHQSRGDAKRTKILIPDSAHGTN
                     PATSTMSGFEVVPVASDARGNVDLESLRAVCDDTVAGLMLTNPNTLGLFDEHVTDIIQ
                     IVHAAGGLVYGDGANLNALLGIARPGDIGFDIMHINLHKTFSTPHGGGGPGSGPVCVT
                     AHLVDFLPDPVVGIVEPGTADMPPLYGFVHPEKSIGRVKAFHGHFGMHVRAFTYISMH
                     GADGLRQIAEYAVLNANYLLSRLRDTYHLAYDRVCMHEFVVEGRWDDAPGVIALDISK
                     RLMDYKYHPPTNYFPLIVHEALMIEPTETESKQALDAFADVLIKIAEEAHTQPELLKE
                     APHITPIGRLDEVKAAKELVLCCWLPEKSE"
BASE COUNT          879 a          987 c          798 g          735 t
ORIGIN      
        1 ccgggccaat taacgccatg gcatttcgag tgccataacg ctcgataagc cattgtgctg
       61 attggtatgc ccccatgctg acaagcgcgt cagcatggtc aaaacgcaca ccctgtgttg
      121 acagatgcaa aatccgccat ccgctttggg cgtgattttt cagaattaga gctttgattc
      181 ccaggcgcgc cagatgcaga cctgtcgtcc ccccggcatt actctcttta attccctggg
      241 ttagtggact tttaccacct atggatatgc ggtcgcagct tgagagcata tgcccaacga
      301 ggagacccgg cgcaaagacc agcacattcc ccgggcttaa tgggtcacag gttggatcaa
      361 tttcgtccag cagaatgcgc gctgaaagac ctcttccacc caggtgctcc cattcaggcg
      421 gcacctggct ctgttccaga gtttgatttt gtaaatcaac tcgccagatt tgcatgcctc
      481 aaacagtctc ctcttctgaa acaaaaggat ccacatctgg taccccgggt ttaatatagc
      541 gtttgattgg gaaatataac gctgctccaa ccagcatggc aaccaaagcc caggatatcg
      601 aggtaattcc ctcctccgca aattgactgt agatggcaaa aacagcaaag atcgtaggcc
      661 ccagcgtaac cagcaccaat cccagggtgc ccccaggaac cttctgccga tgaacatgcg
      721 gatggctgga gcgcagtttc caaagagcaa atagctgcgc cagcaaaacc agcatattca
      781 acaagacatc aaccacaacc agcagttgga atgtctgcaa cgagaatatg gaaaaaagta
      841 cgccgcaaaa tacaatggct acccacggag tgccatattt ggggtgcacc ttgaccatcc
      901 acttgggcat catgccgtct tccgccatag caaacggcac acgcgtccct cccaggctgt
      961 ttccgatgaa caaccccacc atactgagga cagcggcaaa ggccacgctt gatcccattg
     1021 ctctggccag cggactgttc ttaacgccgc tggcaatctc acccaatcgg taagcaatgt
     1081 ctggaaattg ccaaccaacc gagctttgag gattgacttc ccattccgtc agcttttcat
     1141 cggtaacgcc atattcagcc atgacagggc caattccctg accttcttcg tattcttcct
     1201 gcccccagag cagataccga ccgtcattcc cggctccgcc atataaacca gcaaccactg
     1261 gaagcgagta cgttagaata gccgcgacca acacaagggt cattgccagt ggataagtcc
     1321 gcttgggatt cacaatttca tcacctgctg cggtgggcag ttcccagccc atataattcc
     1381 acatcacaac aaataacccc acgctcaacg agctggtgat tccgctaaaa ccgctctgcc
     1441 catcaggtaa caagggaagg gagatcgtaa cgccgccatg aatccagttg tagatcccaa
     1501 caatggtggt caataccagt gggacaagca tggcaatccc aatccagttg gcggtcaaac
     1561 ctgttaaccg cgcccctcgt atattgagcg cactgatacc ccagataata accagagcaa
     1621 ccagccattg cagcacccag ccatctaccc agatgccggc aatttgagtg ccctcgcgca
     1681 ggccggggat catctgcccc agataatagg ctgccaacac cgggtatatg gacacatcca
     1741 cccaggatac aacccagttc atccatccgg ccataaaccc ggcaaagggc ccataagcct
     1801 gcttgaccca atgataatac ccgccttcga cgggcatcat tgagttcatc tcacgcacca
     1861 tgagcatatt ggggatactg aatacgattg gcgtcacggc aatcaggatc aatgccatac
     1921 caggtcccga ccacccaatc aacgattcaa tcccaaaagt accgccacag acggtaaagt
     1981 agatcaatgc aaacaacggc aagagcgtca attctcgttt gagtttgcgg gtttcttgct
     2041 tgatagcagc agcggtttcg ctcatgatag gctcctctca aaaaagaaaa caaaaaggcc
     2101 gcctgatcag gcggcctttt atgatgaggt tactcagact tctcaggcag ccagcagcat
     2161 aacacaagct ctttggcggc cttgacttca tccagcctgc caataggcgt gatatgcgga
     2221 gcttctttga gtaattctgg ttgagtatgc gcttcttcag cgatcttgat caacacatca
     2281 gcaaaggcat ccagtgcctg tttgctttcc gtctctgtag gctcaatcat caacgcttcg
     2341 tgaacgatta aagggaagta gtttgtcggc gggtgatatt tataatccat cagccgtttg
     2401 gagatatcca acgcaatgac gcctggggcg tcatcccaac gaccttcaac aacaaactca
     2461 tgcatacaaa cgcggtcata tgccaggtga taggtgtcac gtaaccgcga aagcaggtag
     2521 tttgcattca gcaccgcata ttctgcgatc tgccgcaggc catccgcacc atgcatcgag
     2581 atgtaagtaa acgcccgcac gtgcatccca aagtgtccgt ggaatgcttt cacgcggcca
     2641 atgctctttt cgggatgcac aaagccatac agcggcggca tgtctgccgt tcccggttca
     2701 acaataccaa caaccggatc cggcaggaaa tccaccagat gcgccgtcac acagaccggc
     2761 ccagaacccg gaccgccacc gccatgtggg gttgagaaag tcttgtgcag gttgatatgc
     2821 atgatatcaa agccaatatc acccgggcga gcaatcccga gcagtgcatt caggttggcg
     2881 ccatcaccat aaactaaacc gccagccgcg tgcacaatct ggatgatatc ggtgacgtgc
     2941 tcatcaaaca accccagcgt attcgggttg gtcagcatca atccagcaac ggtgtcatcg
     3001 cacactgctc gcagagattc caggtccaca tttccccgtg catcgctcgc tactggcaca
     3061 acctcaaatc cgctcatggt ggacgtggca gggtttgtgc cgtgggctga gtccggaatg
     3121 aggattttgg tacgttttgc gtcaccgcga ctctggtgat atgcccggat aatcatcacg
     3181 cccacgaact ctccgtgcgc gcctgcagct ggctgcaggg tgcagccagc aaagccagag
     3241 atttcctgta agtattgctg gagttcatac atcaacgcca ggttgccctg aaccgtctca
     3301 atcggctgca gcggatgcgt gtacgcaaaa ccggataacc gggcagtatc ctcattgatt
     3361 ttgggattgt atttcatcgt gcaggatccc agaggatag
//
LOCUS       DAOPVS010000187         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_28822_length_3364_cov_59.317014, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000187 DAOPVS010000000
VERSION     DAOPVS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28822_length_3364_cov_59.317014"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKL11_10335"
     CDS             <1..426
                     /locus_tag="PKL11_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNZ14037.1"
                     /translation="KVISTKGEANSLAASQYHLDKAFQLNGHEAAYVAIGDEEPTQRL
                     IKKLEGVPFLVVQAGYTSQLTAMADVVLPAAIWLEQDGHYLSLEGRLQFAHKSLQAGA
                     DVRSNAEIFQNLAGCLGVTIKDDWRAALMLRQSPVTIIG"
     gene            450..992
                     /locus_tag="PKL11_10340"
     CDS             450..992
                     /locus_tag="PKL11_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNZ14038.1"
                     /translation="MGKPKIASDWLCGCAGCHMSLLDMDERLAHIVELADLRATPITD
                     LKEPDETGVDVGVLEGGVNNSTNEEVAHRMRKRCGILVALGDCAVFGGVPAMRNFFTV
                     EEALRRAYVETESTDSGGKIPDDPELCQQTRVRALSEVVPVDIHVPGCPPDADVIYYV
                     LSELAQGRRPVLKDDKLHWH"
     gene            1010..2443
                     /locus_tag="PKL11_10345"
     CDS             1010..2443
                     /locus_tag="PKL11_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNZ14039.1"
                     /translation="MVAQKITIEPVTRIEGHAKITIRMNDDGTVNHAYLHVNEFRGFE
                     KFCEGRMVFEMPYITPRICGICPVSHHLAAAKAGDAVMGCPPPRPAELLRELMHMGQV
                     VQSHGMHFFELAGPDLLLGFDRDPATRNVVGLLQANADLTVKAINLRKWGQEIIRILG
                     GRRIHPVFAIPGGVNKALTPAERDTILAGMDEAIATTQIGIQIIKDWAYQNAEDIAKF
                     GVFPTGYFGLVTPQNSLELYQGQIRLTGRDGKQLEKFNASHYLDYIAEHVENWSYLKF
                     PYYKPMGFPGGVYRVGPLGRLNNSDQIDTPLANAEHKIWKTLNGGRPVENTLHYHYAR
                     LIEVLFAIERVGVLLSDPDVMSTDLLNTRHDFQGEGVGVIEAPRGTLLHHYFAKPNGQ
                     LERVNLIVSTGHNNFAMCEAVDSVAKTYINGQEIREGLLNRVEAAVRAHDPCLSCSTH
                     AIGQMPMVVNIVDNDGNLIQTLSRGVD"
     gene            2430..2993
                     /locus_tag="PKL11_10350"
     CDS             2430..2993
                     /locus_tag="PKL11_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNZ14040.1"
                     /translation="MALINSTFSKHSDFLLSPETGRAGSPFKRTLILGYGNPDRQDDG
                     VAVHILEMLAKRLELDFDAQLTEVEDLNAPMALLFALQLMPEHAETVAVYERVCFVDA
                     HTGSVPNDLNIQTVQPVYQKSPLTHHMTPDACLSIVETISGKAPEAILVSVRGYAFQF
                     TQELSSPTQALAEEAVECILAWMNTPQ"
     gene            3009..>3364
                     /locus_tag="PKL11_10355"
     CDS             3009..>3364
                     /locus_tag="PKL11_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNZ14041.1"
                     /translation="MSTPSGSSSDALPPQEMAVKAEAVGIRKAQGTFVNLFLLGILAG
                     AYVALGGLFATTVSAGASGILPYGLTRLLMGVVFSLGLILVVVGGAELFTGNTLIAIA
                     WASGKVSTGGLLRNWA"
BASE COUNT          741 a          877 c         1000 g          746 t
ORIGIN      
        1 aaagtgatca gcaccaaggg cgaggcgaac agcctggctg cttcgcagta ccacctggat
       61 aaggcgttcc aactcaacgg tcatgaggct gcctatgtgg ctattggcga tgaagaaccg
      121 acccagcgtc tgatcaagaa gctggaaggt gtgccgttcc tggttgttca ggcgggctac
      181 acgtcacaac tcaccgccat ggcggacgtg gttctgccgg ccgcaatctg gttggagcag
      241 gacgggcact atctgagtct ggaaggccgc ttgcagtttg cccacaagtc gctgcaggca
      301 ggcgcagatg tacgcagcaa tgccgaaatt ttccagaatc tggctggctg cctgggtgtt
      361 acaattaagg atgattggcg tgccgctctg atgctgcgtc agtcgccggt caccattatt
      421 ggttaattga gcgagataag gagaattaca tgggaaaacc caagattgct tctgactggt
      481 tgtgcggctg tgcaggctgc cacatgtctc tgctggacat ggatgagcgg ctggcccata
      541 ttgtggaatt ggctgactta cgtgccaccc caatcactga tttgaaagag ccggatgaaa
      601 ccggcgtgga tgttggcgtt cttgaaggcg gcgttaataa cagcaccaat gaagaagttg
      661 cccatcggat gcgcaagcgc tgcggtattc tggttgccct gggcgactgc gctgtattcg
      721 gcggcgtgcc ggcaatgcgc aatttcttca ctgttgaaga ggccctgcgc cgggcgtatg
      781 tagagactga aagcaccgac tcgggcggca agattccgga cgacccggaa ctctgccagc
      841 agacccgggt gcgagccctg agtgaagttg tgccggttga catccatgtt ccgggatgcc
      901 cgccggatgc tgacgtgatt tattacgttc tcagcgaact ggcacaggga cgccgccctg
      961 tactcaagga tgacaagctc cactggcatt agaagtaaag aggtagaaaa tggtagcaca
     1021 aaaaattacg attgagcccg tcacccggat tgaagggcac gccaagatca ccatccgcat
     1081 gaacgatgat ggcacggtga atcacgcgta cctgcatgtg aacgaattcc gcggttttga
     1141 aaaattctgc gaaggccgca tggtctttga aatgccctac attaccccgc gcatctgcgg
     1201 catctgcccg gtgagccatc acctggcggc ggctaaagct ggcgacgcgg tgatgggctg
     1261 cccgccgccc cgcccggctg aactgctgcg cgaattgatg catatgggtc aggttgtgca
     1321 gtcgcatggc atgcacttct ttgaactagc cggccccgat ctactgctgg gcttcgaccg
     1381 cgacccagct acacgcaatg tagtaggtct gctgcaggcc aatgccgatt tgaccgtcaa
     1441 ggccatcaac ctgcgcaaat ggggccagga aatcatccgc attctgggcg gacgccgcat
     1501 tcacccggtg tttgccattc ccggcggtgt gaacaaagcg ctgaccccgg cagaacgcga
     1561 cactattctg gctggtatgg atgaagccat tgccacgacg cagattggta ttcagatcat
     1621 caaagactgg gcttaccaga acgctgaaga cattgctaag tttggcgttt tcccaaccgg
     1681 atactttggc ctggtgacac cgcagaacag cctggaactt taccagggcc agatccggct
     1741 gaccgggcgc gacggcaaac agcttgagaa gtttaatgcc tcgcattatc tggattacat
     1801 tgctgaacac gttgaaaact ggtcgtacct caagttcccg tactacaagc cgatgggctt
     1861 cccgggtggt gtgtaccgcg tgggtccgct gggacgtttg aacaattctg accagattga
     1921 cacgccgctg gcgaacgcgg agcataagat ctggaagacc ctcaacggtg gccgcccagt
     1981 tgaaaacacc ctccactatc actatgcccg cctgattgaa gtcttgtttg cgattgagcg
     2041 cgtgggcgta ctgttgagcg accctgatgt tatgagcacc gacctactga acacccggca
     2101 cgacttccag ggcgagggtg tgggcgtcat cgaagcgccg cgcggcaccc tgctgcacca
     2161 ttactttgcg aagccgaacg gccagcttga acgcgtgaac ctgatcgttt cgacgggaca
     2221 caacaacttt gcgatgtgcg aagcagttga cagcgttgcc aagacctata tcaacggcca
     2281 ggaaattcgc gaaggactgt tgaaccgggt tgaagctgct gtgcgggcgc acgacccgtg
     2341 cctgtcttgc tctacacatg ccattgggca aatgccgatg gttgtcaata ttgtggataa
     2401 cgacggcaat ctgattcaaa ccctcagccg tggcgttgat taattcgacc ttctcaaaac
     2461 attcagattt cctgctctct ccggaaacgg ggagggcagg aagtcctttt aaacgcacgc
     2521 tgatcctggg gtatggcaat ccagaccggc aggatgacgg tgtggcggtg catattctgg
     2581 aaatgctggc gaagcggctg gaactggact ttgatgcgca gttgaccgag gttgaagact
     2641 tgaatgcccc gatggcgttg ctgtttgccc tgcagttgat gcccgaacac gctgaaactg
     2701 tggccgtcta tgaacgggtt tgctttgtgg atgcacatac gggtagcgta cccaatgatc
     2761 tcaatatcca aacggttcag ccggtctatc aaaaatcacc gctgacacat catatgacac
     2821 cggatgcctg tctctcgatt gtggagacca tttcaggaaa agcgccggaa gctatcctgg
     2881 tgtcggtgcg tggatatgct tttcaattta cgcaggaatt atccagtccc acgcaggcgc
     2941 tcgcagaaga agccgttgag tgcatcctgg cgtggatgaa caccccgcaa taagcccgga
     3001 ggtttaagat gagcactccg tcaggttctt caagcgatgc tttaccgccg caagagatgg
     3061 cggtcaaagc cgaggcggtg ggtattcgga aggcacaggg gacgtttgtc aacctgtttt
     3121 tattggggat tctggcaggg gcctatgttg ccctgggtgg actgtttgcc actacggtat
     3181 cggcaggggc gagcgggata cttccatatg gactgacgcg tttgctgatg ggggttgtct
     3241 tctcgctggg attgattctg gtggtcgttg gcggggcaga gctgtttacc ggcaacacgt
     3301 taattgccat cgcctgggcc agtgggaagg tgagcaccgg cggattgctg cgcaactggg
     3361 cgat
//
LOCUS       DAOPVS010000188         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_28876_length_3359_cov_57.982748, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000188 DAOPVS010000000
VERSION     DAOPVS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28876_length_3359_cov_57.982748"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKL11_10360"
     CDS             <1..439
                     /locus_tag="PKL11_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14042.1"
                     /translation="YRGKSGKTKAYGIPLQSGLYIPGMEVEIQPCDEAGKRLTGPATM
                     KVLAATIGDPVADAPEKQPEKRYLSVTLIYRMQQPYDGENPRYGCCIPAVNGVRSSSH
                     VVDVWPCDENGGIHPGFNVSPVPVSASVILEPFRTAPGYGDSV"
     gene            738..2018
                     /gene="iscB"
                     /locus_tag="PKL11_10365"
     CDS             738..2018
                     /gene="iscB"
                     /locus_tag="PKL11_10365"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HNZ14043.1"
                     /translation="MQYVFVLDKDRKPLDPCHPARARQMLSEGRAAVIRRHPFTIILT
                     DRTQAESVVHEHRVKIDPGSKTTGLAVVREGDGKVVWAGEITHRGQVIRDALLSRRAI
                     RRGRRHRHCRYRPARFNNRRRKKGWQPPSLESRLSNVQTWVKRLLRCAVVTAISLELV
                     KFDTQSMQNPEISGVEYQQGELAGYEVREYLLEKWGRKCAYCGKTDTPLEVEHIIPHT
                     RGGTNRVSNLTLACRECNMRKGRQTAAEYGHPNIQSAARLPLKDAAAVNATRWELWRR
                     LAQIGLPVECGTGGRTKYNRVHQGLPKTHWMDAACVGRTGEKVFVPNNLTALGIKSCG
                     HGRRQMCLVDKYGFPRTSAKKSGRIYGFKTGDIVEARVPKGKYAGVHYGRVVVRASGW
                     FQIGNANVSHRGCRIVQRADHFEYTRKECGSSTR"
     gene            2051..3034
                     /locus_tag="PKL11_10370"
     CDS             2051..3034
                     /locus_tag="PKL11_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HNZ14044.1"
                     /translation="MTAMAATTTMTQPKKGWKSRSNETGDWQQQAEDKRNALMQSITD
                     AIASLAAETDEVRASETLARYFKTVSQFHRYSLHNQILIWLQKPDATLVAGYRTWQQK
                     FNRCVKKGEKGIAILAPRIYKPKEDEDSKEGGERTYEGGTVLLSTLSSEEGDQPAPRR
                     RIYFTTVYVFDVSQTEGDPLPDEPEWRERAKDLDFEQRLTAFAQSRGITVEIVDDMDG
                     AEGRSFGGKIQLLPQSGSHTFVHELAHELYAHCKPEVREQTTRQQREIEADAAAHVVC
                     QHFGIPSASANYLALWQAGKEDILGCMERVRGVAIELIEAIEGTHQHEETD"
     gene            3098..>3359
                     /locus_tag="PKL11_10375"
     CDS             3098..>3359
                     /locus_tag="PKL11_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14045.1"
                     /translation="MPEIDSHKIILEDGTEISVYALTHGSNFQPEHLAGILANVYNQY
                     GVGDFNLRLAKALLERHRTLQQSVVRAFVAVMVHISKLCAAMP"
BASE COUNT          839 a          902 c          999 g          619 t
ORIGIN      
        1 ctatcgcgga aaaagcggaa aaacaaaagc atatggtatc ccgctgcaaa gcgggcttta
       61 catccctggt atggaagtgg aaatccagcc ctgtgatgaa gccggcaagc ggctcaccgg
      121 gccggcgacg atgaaagttc tcgccgcaac cattggcgat ccggttgctg atgcacccga
      181 aaagcagcct gaaaagcggt atttgagcgt cacgctgatc taccgtatgc agcagcctta
      241 tgatggggaa aatccccgtt acgggtgctg catcccggca gttaacggtg tgcgatcatc
      301 atcacatgtg gttgatgtct ggccctgcga cgaaaacgga ggtattcacc ccggtttcaa
      361 cgtgtcaccg gtgcctgtct ccgcatccgt aatcctggag ccgttcagga ccgcacccgg
      421 ttacggggat tctgtgtgat gtctgcggcc agcctgtgga ccccggtcaa cgaccacctg
      481 ctaaagcagg tggcttgtag cggagacgcc acaagcccag gttgaccagc ctaagcccga
      541 atatcgggct acgttattgg gaaagttgaa gaacacacgt tgggatgtat tcagccagtc
      601 ccaacctctg caacgggcaa gttaaacatc gcgagggtct agcgaagtgc ttgtccggtg
      661 tactgtccaa taacattggc gaggcgaact ttacccccga aaggggagaa cactcgtaat
      721 gagaaaggag aaaaacaatg cagtatgtgt ttgttctgga caaagaccgg aaaccccttg
      781 acccgtgcca cccggcgcgg gcgcggcaga tgttgagcga gggtcgtgcg gcagtaatac
      841 gccgccaccc cttcaccatc atcctgacgg atcgaaccca ggccgaatcg gtcgtgcatg
      901 aacaccgggt caaaattgat ccagggagca aaacgaccgg cctggcggtg gtgcgtgagg
      961 gagacggcaa ggtggtatgg gccggagaga tcacccatcg gggtcaggtc attcgtgacg
     1021 ccctgctatc ccgccgggcc atccgacgag ggcggagaca ccgccactgt cgctaccgtc
     1081 cggcgcggtt caacaaccgc cggaggaaga aagggtggca gccgccgagt ctggaaagtc
     1141 gcctgtccaa cgtccaaacc tgggtcaaac gcctgctccg ctgcgcagtt gtaacggcga
     1201 tctcgctgga actggtgaag tttgacacac agtccatgca gaaccccgaa ataagcggag
     1261 tggaatatca gcaaggcgaa ctggccggat atgaagtccg ggaatatctg ctggagaagt
     1321 ggggccggaa atgcgcatat tgcggcaaaa ccgacacccc gctggaagtg gagcatatca
     1381 taccccacac caggggtggc acgaatcgag tgagcaacct gacgctggcg tgtcgggagt
     1441 gcaatatgag aaaaggccgc caaaccgcag cggagtatgg acatcccaac atccaaagtg
     1501 ccgcccgact ccccctgaaa gacgcggctg cggtcaacgc aacgcgctgg gagttatggc
     1561 gcagactggc acaaatcggc ttgccggtgg aatgtggtac aggcgggcgc acgaagtaca
     1621 accgtgtgca tcagggattg cccaagacac actggatgga tgccgcctgt gtcggtcgga
     1681 caggagaaaa agtgtttgtc ccaaacaacc tgactgccct gggaataaag tcctgcgggc
     1741 atggccggcg tcagatgtgc ttggtggata agtatggctt cccccgtaca tcggcgaaga
     1801 agtctggccg gatatatggc ttcaagacag gcgacatagt ggaagcgaga gtgccgaaag
     1861 ggaaatatgc tggcgtccat tatgggcgtg tcgttgtccg ggcgagtgga tggttccaaa
     1921 ttggcaatgc caatgtgtcc catcgcggct gtcggatagt acaacgagca gatcattttg
     1981 aatacacaag aaaggagtgc ggctcctcca cccgctgaag cggagtggtt tctgccgcaa
     2041 agaattatct atgaccgcaa tggccgcaac gaccaccatg acccagccca agaagggctg
     2101 gaaatctcga tcaaacgaaa caggcgactg gcagcagcag gctgaggata agcgcaacgc
     2161 gctgatgcag tccatcaccg atgcgattgc gtccctggct gctgaaaccg atgaagtccg
     2221 ggcgagtgaa acactggctc gctacttcaa aacggtcagt cagtttcacc gatacagcct
     2281 gcacaaccag attctcatct ggctccaaaa gccggatgcg accctggtcg ccggctaccg
     2341 gacctggcag cagaaattca atcggtgtgt aaagaaaggg gaaaagggta tcgctatcct
     2401 ggcccctcgg atttacaagc cgaaagaaga tgaagactcc aaagaaggtg gtgaacggac
     2461 ttacgaagga ggcacggttc tcctgagtac cctttcgagc gaagaaggcg atcagccggc
     2521 tccccgccgg cgcatctact ttacgacggt gtacgtcttt gatgtatccc aaaccgaggg
     2581 tgatccttta ccggatgaac ccgaatggcg ggaacgcgcc aaagaccttg actttgagca
     2641 gcgccttacg gcgtttgccc aaagccgggg aattaccgtt gaaatcgtgg atgacatgga
     2701 cggcgctgaa ggccgttcct ttggcgggaa gatccaacta ctgccccaat cgggcagcca
     2761 tacattcgtg catgaactgg cgcacgaact gtatgcgcat tgcaaacccg aagtccggga
     2821 gcaaaccacc cggcagcagc gggaaatcga agcggatgca gcggcgcatg tcgtctgcca
     2881 gcatttcggt atcccctctg ccagcgcaaa ttacctggcg ctctggcagg ccgggaaaga
     2941 ggatatcctg ggctgcatgg agcgggtccg gggagtggct atcgaattga tcgaagccat
     3001 tgaaggtaca caccagcacg aagaaactga ctagaacacg ctccctgccc tttaccgggc
     3061 ggggagcttt acaaaaataa gaaggagaat acaagaaatg cctgaaattg atagccataa
     3121 aatcattctc gaggacggca cggaaatctc cgtgtacgcc ctcacgcatg gctcaaactt
     3181 ccagcctgaa cacctggccg ggattttggc gaatgtgtac aaccaatacg gggtgggaga
     3241 cttcaacctg cgcctggcga aagcgctgct ggaacgacac cgtaccttgc agcaaagcgt
     3301 ggtgcgggct ttcgttgcgg tcatggtcca tatctcaaag ttgtgtgcgg cgatgccgt
//
LOCUS       DAOPVS010000189         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_30631_length_3219_cov_42.460493, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000189 DAOPVS010000000
VERSION     DAOPVS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30631_length_3219_cov_42.460493"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PKL11_10380"
     CDS             <1..1381
                     /locus_tag="PKL11_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ14046.1"
                     /translation="LSLTVAVTLLWSLLRSPLLYRSTTQQLRLISALTARRLQSQPDL
                     QSDKISRQLDLAAQESALRLAIINPSGQVTYDSGAGQYPALRLTDLTLSPVIQMDVPV
                     RTFRVPQSRVWFYVLQPLPGDYILVTTTPRPQQTLRGILRDEMLGPLFFSGGLALLLA
                     FIFSAFMSRWIARPLQQLAEGARSMAKGQYPILTPSGPREIFDLTQSLNDLSQQVQTS
                     QQAQRDLIGNISHELKTPITAIQGFSQAILDGTVASPAALQEAATVMSVESDRMHRLV
                     SDLLTLTRLEGGIADLHRAPVDLNALLHHAQARFSLQARQSGVELVVLSGAVPPIIGD
                     GDRLSQVLANLVDNALKYTPPGGKITLSAAAEGDWAILRITDTGPGIPADEQERIFER
                     FYQLDKSRRGGSGRGVGLGLPIAREIIRAHSGSLWVESSPGQGSVFVIKIPFVLPDDI
                     TLNQKANNA"
     gene            1374..2375
                     /locus_tag="PKL11_10385"
     CDS             1374..2375
                     /locus_tag="PKL11_10385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ14047.1"
                     /translation="MPEVSVIVPCFNEEKTIVLLLDALYRQTQPRADLEIIIADGFST
                     DRTRTVIAEYQRTHPDLSIRVVDNPARTIPSGLNAAITAAHGEFIVRLDAHSVPASDY
                     VERCLNDLKSGLGDNVGGIWEIRPGSPTRIARAISLAAAHPFGVGDASYRLGAASGPV
                     DTVPFGAYRRSLVDKIGLYDPTLLSNEDYEFNTRIRQSGGIVYLDSLIRCIYYSRATL
                     PALARQYSRYGYWKYQMLRRYPGTLRWRQALPPVFVFGMLLLALLAPWWSLAALTFLV
                     VTLLYFITLGAGSMPLARRYHDPALIYAIPLAIIVMHFCWGGGFLWSIISTVFRRTG"
     gene            2385..>3219
                     /locus_tag="PKL11_10390"
     CDS             2385..>3219
                     /locus_tag="PKL11_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14048.1"
                     /translation="MGIYTKPLKRTWRLNSGERKIILFVGDVIVSVLALMIALYFWAQ
                     SPQEWLNFSWQFLGERIPGWYYLLPVIWMLLLVELYDVDRSSNRLETFKGIAIAAVIA
                     VILYLIVYFTSEPNSLPRRGVASFILGAFLLTLGWRMLYIAIFTAPRFMRRVIIVGAG
                     RAGTTLLQVIKSTWPPPFYLVGLVDDDPEKMGAEFLDYRVLGSSADLMALIKEHQISD
                     LIFAISGEMRPETFRVLLEAEEHGVQVTTMPAVYEQLLGRVPIFLLQSDWILRSFLDQ
                     AH"
BASE COUNT          599 a          989 c          799 g          832 t
ORIGIN      
        1 cctgtcgctg accgttgctg tcacattgct ctggtcgctt ttacgcagtc cattgctgta
       61 ccgcagcaca acccagcagc ttcggcttat atctgcattg accgcccggc ggttgcaatc
      121 ccaacccgac cttcagtccg ataaaatttc ccgtcaactg gaccttgcag ctcaagaatc
      181 tgccctgcgg ttggcgatta tcaatccctc agggcaggta acctatgaca gcggcgctgg
      241 acagtacccc gcgttgcgcc ttactgatct caccctgtcc ccggtcatcc agatggatgt
      301 ccctgtccgc acatttcgcg tccctcaatc ccgcgtctgg ttttacgtac ttcaaccgct
      361 gcctggtgat tatatcctgg tcacaacaac gccgcgccca cagcaaacct tgcgcggcat
      421 cctgcgagat gaaatgttag gtccattgtt cttttcgggc ggtttggcac tgctgctggc
      481 cttcatcttc agcgccttca tgtcgcgctg gatcgctcga ccccttcaac agcttgccga
      541 aggcgcccgg tcaatggcta aaggtcagta ccccattctc accccctcgg gcccgcgcga
      601 aatatttgat ctcacccaat ccttgaacga tctgagccaa caggtgcaaa cctcccagca
      661 ggctcaacgc gacctgattg gcaacatttc gcatgaatta aaaaccccca tcaccgccat
      721 tcaaggcttt tcacaggcca ttctggatgg cactgtggcc tctcctgctg ccctgcagga
      781 agccgccacc gtgatgagcg ttgaatcaga tcgaatgcac cggctggtct ccgacctgct
      841 cactctcaca cgcttggaag gtggcatcgc agacttgcac cgcgcccccg tagatttgaa
      901 cgccctgctc caccatgctc aggcgcgctt ctccctgcaa gcccgccaat cgggcgttga
      961 attagttgtc ctgtcaggcg ccgtgccgcc aatcatcggc gatggcgacc gcctttcgca
     1021 agtcctggct aatctggtag ataatgcgct caaatacacc cctcctgggg gtaaaattac
     1081 cctcagcgct gccgcagaag gcgactgggc gatattgcgg attaccgata caggccccgg
     1141 catccccgcc gacgaacagg aacgcatctt tgaacgtttt tatcagctcg ataaatcccg
     1201 ccgcggcggt tcggggcggg gggttgggct gggtctgccc attgcccgcg aaattattcg
     1261 cgctcattcc ggctccctct gggtagaatc atctccaggc cagggcagcg tttttgtcat
     1321 caaaatcccc ttcgtccttc cagacgatat aaccctgaat caaaaggcca ataatgcctg
     1381 aagtctctgt gatagtccct tgttttaatg aagaaaagac catcgtgtta ctgctggatg
     1441 cgctttaccg ccagacccag cctcgtgcag atctggagat cattattgcc gatggcttct
     1501 ctaccgatcg aacccgtacg gtgatagctg aatatcaacg gacccacccc gacctgtcta
     1561 tccgggtggt ggataaccct gctcggacga tcccgtccgg cctgaacgcc gccattaccg
     1621 cagctcatgg agagtttatt gtccgcctgg atgcccattc ggtcccggct tcagattatg
     1681 tcgaacgctg cctcaatgat ctgaaatccg gcctggggga caatgtcggt ggcatctggg
     1741 aaattcgacc cggcagcccc acacgtattg cccgtgccat ctcactggct gctgcccacc
     1801 ccttcggcgt aggcgatgcc tcttaccggc tgggcgccgc ctctggcccg gtcgataccg
     1861 tcccgtttgg cgcctaccgc cgttctttgg tggataaaat tggtctctac gacccaaccc
     1921 ttttgagtaa tgaggactat gaatttaaca ctcgcattcg gcagtctggc ggcatcgtct
     1981 acctcgattc gctgattcgc tgtatctatt attcccgggc cacactgccg gctctggcgc
     2041 ggcagtacag ccgctacggc tactggaagt atcaaatgct gcggcgctat cccggcactt
     2101 tgcgctggcg tcaggcgctc ccgccagtat ttgtcttcgg catgttgctc ttggccctcc
     2161 tggccccctg gtggtctctg gcagccctga cctttttagt agtaaccctc ttatatttca
     2221 tcactcttgg tgctggcagc atgccgcttg ccaggcgcta tcatgatcca gcgctgattt
     2281 atgccattcc cctggcaatc atcgtcatgc atttttgctg gggtggtggc tttttgtgga
     2341 gtataatttc aaccgtcttt cgcagaacgg gttaaaatgg agctatgggc atttacacca
     2401 aacccttaaa acgtacctgg cggttgaatt ctggcgagcg caaaatcatc ctctttgttg
     2461 gcgatgtgat tgtatctgtc ctggccctga tgattgcgct ttatttctgg gctcagtctc
     2521 cccaggaatg gcttaacttt tcctggcagt tccttggaga acgcatcccc ggctggtatt
     2581 atttattgcc cgtcatctgg atgctcctgc ttgtcgaatt atatgatgtg gaccgctcca
     2641 gcaatcgcct tgagacgttc aaaggcattg ccattgctgc ggttatcgct gtaattcttt
     2701 acctgatcgt ctatttcacg tctgagccta attcactgcc ccgtcgtggt gtggccagct
     2761 ttatcctggg cgcttttcta ctcaccctgg gctggcgcat gctctacata gccattttta
     2821 ccgccccacg cttcatgcgc cgtgtgatca ttgtcggcgc tgggcgggcc ggcaccacct
     2881 tgttgcaggt catcaaatcg acctggccgc cgccctttta tctggttggc ctggtggatg
     2941 atgatcccga aaaaatgggc gccgaattcc tggattatcg cgtcttgggc tccagcgccg
     3001 atttgatggc actgatcaaa gagcaccaga tctctgacct gatttttgcc atctcgggcg
     3061 aaatgcgccc tgagacattt cgggtccttc tggaggccga agaacatggt gttcaggtca
     3121 ccaccatgcc tgccgtctat gagcagctgc tcgggcgcgt gcctatcttt ttgcttcaat
     3181 ccgattggat tctgcgctcg tttttggatc aggctcacc
//
LOCUS       DAOPVS010000190         3217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_30659_length_3217_cov_55.205250, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000190 DAOPVS010000000
VERSION     DAOPVS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30659_length_3217_cov_55.205250"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..798)
                     /locus_tag="PKL11_10395"
     CDS             complement(25..798)
                     /locus_tag="PKL11_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ14049.1"
                     /translation="MKRREIPRRFWLAGALLLAFILLPLLSLLFSSSPAALAQTLVDA
                     EVLRSLGVTFAGAGIATALGLLLGLPLAFVLARASFPGKGWVEALVDLPVIIPHTAAG
                     VALLLVFGRQGVLGRWLTPLGLYFTDNLAGIVVAMLFVSLPLLVHPARAALAQLDREV
                     EQSARVDGASGWQVFWQIMLPQAWRGVFSGALMMWARGISEFGAVVILAYHPKVAPVL
                     VFERFNGFGLDAALPVAVILVGVALLVFGSLRWLAREAG"
     gene            complement(795..1865)
                     /gene="wtpA"
                     /locus_tag="PKL11_10400"
     CDS             complement(795..1865)
                     /gene="wtpA"
                     /locus_tag="PKL11_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HNZ14050.1"
                     /translation="MKKSTLLFCLLALTLTLLGGCKPPGKTPLVVFCAGSLITPFDAL
                     EKAFEAQYPDIDVQNEFHGSIQVIRHVTELHEPIDVVATADYALIPMLMYAVNDPDTG
                     QPYADWYIRFAGNRLGLAYTPQSAYAAEITPENWTEILARPDVRVGIADPRFDAAGYR
                     ALMAYTLAQDLSDQPTLFFDQFAGQFRYPINLEPGADGSTIIHVPEVLENQTGAHVIL
                     RGASIQLIALLESGDLDYAFEYETVIQQHNLELLTLPDALNLGSPDYAAEYARATVQL
                     DFQRFASVNPVFQGAPIGYGITIPGNAPHSAAAEQYIAFLLSPEGRAVMAAAQHPLLD
                     APTANGYANLPVSLQPLVTAEP"
     gene            complement(1968..>3217)
                     /locus_tag="PKL11_10405"
     CDS             complement(1968..>3217)
                     /locus_tag="PKL11_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNZ14051.1"
                     /translation="TEGYAGTKSSTGKITVPELYRGVLLTDLLDEVDGLKSDQAIQIT
                     AEDGYAMTFSPDQVANGEFITYDPGTGDEIAAPGPLQVLLAYERAGAPLNAEEEGALR
                     LMVISPEGNQIVDGHWAIKWVSAISYKPLTADWNLELTGAITDTVDRGTFESCSTGAC
                     HQAEWTDDKAQTWTGVPLWELVGRVDDETKHGDDAFSDDAASAGYTVDVISADGYIVT
                     LDSAAIQHNDNILVAYQVNGNVLTDEDFPLRLVGTDLSKKEMAGGLVQIVVNFGAAPV
                     EPTATTAPEPTATTAVAQPTNPDAALTIKGLVTAETAWTMDELHGMEVVTLNVVHPKK
                     GDQTAEGVRLNALLDLAGLKPEATTLVITASDGYIAEAALADVRACADCLIAFNESGQ
                     LKSVMPGFESSFWVKDVVSIEVK"
BASE COUNT          605 a         1030 c         1026 g          556 t
ORIGIN      
        1 gaacctggaa cgtggaacgc ttttctaccc tgcttcccgc gccagccagc gcaggctgcc
       61 aaacaccagc agcgccaccc ccaccagaat caccgccacc ggcagcgcgg catccagccc
      121 aaagccattg aagcgctcaa acaccagcac cggcgccacc ttggggtggt aggccaaaat
      181 caccacggcc ccaaactcgc tgatgccgcg cgcccacatc atcaacgccc cgctgaagac
      241 cccgcgccag gcctgcggca gcataatctg ccagaacacc tgccagcccg aggccccatc
      301 cacccgggcc gactgctcca cctcgcggtc caactgggcc agggcggctc gcgccggatg
      361 caccagcagc ggcaggctga cgaacagcat ggccaccaca atgcccgcca gattatcggt
      421 aaagtagagg cccagcggcg tcagccagcg gcccagcacc ccctgacgcc caaagaccag
      481 cagcagcgcc acccccgccg ccgtgtgcgg gatgatcacg ggcagatcca ccagcgcctc
      541 cacccagccc ttgcccggga agctggcccg cgccagcaca aaggccagcg gcaggcccag
      601 cagcaggccc agtgcggtgg caatgcccgc cccggcaaag gtgacgccca gcgagcgcag
      661 cacctcggca tccaccaggg tttgggccag cgccgccggg ctggatgaga acagcaggct
      721 cagcagcggc agcaaaatga aggccagcag cagcgccccg gccaaccaga aacggcgggg
      781 aatctcgcgg cgcttcatgg ctcggcggtc accagcggct gcaggctgac aggcaggttg
      841 gcgtaaccgt tggcggtggg cgcatccaga agcggatgct gggcggcagc catcaccgcg
      901 cgcccctcag ggctgagcag aaaggcgata tactgctcgg cggccgcgct gtgcggcgca
      961 ttgcccggaa tggtgatgcc gtagccaatc ggcgcaccct gaaaaaccgg attcacactg
     1021 gcaaagcgct gaaaatcaag ctggacggtg gcccgggcat actcggcggc atagtcgggg
     1081 ctgcccaggt tcagcgcgtc gggcagggtc agcagttcca ggttgtgctg ctggatgacc
     1141 gtctcgtact caaaggcgta atccaggtcg ccggattcca gcagggcaat caactgaatg
     1201 ctggcgccgc gcaggatcac atgcgcgccg gtttgattct ccagcacctc tggcacatgg
     1261 atgatggtgc tgccatcggc ccccggctcc aggttgatcg ggtagcggaa ctgccccgca
     1321 aactggtcaa agaacagggt gggctggtca gacaaatcct gtgccagggt gtaagccatc
     1381 agggcgcggt agccagcggc atcgaagcgc gggtcggcaa tccccacgcg cacatcgggg
     1441 cgggccagaa tctccgtcca gttctcgggg gtaatctcag cagcgtaggc gctctggggg
     1501 gtgtaggcaa ggcccaggcg gttgcctgca aagcggatat accagtcggc gtagggctgc
     1561 ccggtgtcgg ggtcgttcac ggcatacatc agcatgggga tgagcgcata gtcggcggta
     1621 gccaccacat caatcggttc gtgcagctct gtcacatgcc ggatgacctg gatgctgccg
     1681 tgaaactcgt tctgcacatc aatatcgggg tattgggcct caaaggcttt ttccagcgcg
     1741 tcaaacgggg tgatgaggct gcccgcgcaa aagactacca gcggggtttt gcccggcggt
     1801 ttgcagccgc ccagcagggt cagggttaag gccagcaggc agaacagcag cgtagatttt
     1861 ttcatgtcag gctccaggtg ggggaaaaaa gacctcccct tcgctcccgc gccggggaag
     1921 gaaggggagg tgtgcggggg tgggctaaca gcccggcgcc cttgatttta cttgacttcg
     1981 atggaaacca cgtctttgac ccagaagctg ctttcaaagc cgggcatcac actcttgagt
     2041 tgcccggatt cgttgaaggc gatcagacag tcggcgcagg cgcgcacatc tgccagggcg
     2101 gcttcggcaa tatagccatc gctcgcggtg atgacgaggg tggtcgcttc gggtttgagg
     2161 cccgccaggt cgagcagggc attcaggcgc acaccttcgg cggtctggtc acccttcttg
     2221 gggtgcacca cgttcagcgt caccacctcc atgccgtgca gctcgtccat tgtccaggca
     2281 gtttccgccg tcaccagccc tttgatcgtc agggcggcat cggggttggt gggctgggcc
     2341 actgccgtgg tggcggtggg ttcgggggcg gtggtggctg tgggctccac tggcgcggcg
     2401 ccaaaattca ccacaatctg aaccaatccg ccagccattt ccttcttgct caggtctgtg
     2461 cctaccagtc gcagcggaaa atcttcatcc gtcagcacat tgccgttcac ctggtaagcc
     2521 accaggatat tgtcgttgtg ctggatggcg gcgctgtcga gcgtgacgat gtagccatcg
     2581 gccgagatca catccacggt gtagccggcg gatgccgcgt catctgagaa ggcatcgtcg
     2641 ccgtgtttgg tttcatcatc cacgcgaccc accagctccc agaggggcac gccggtccag
     2701 gtttgggcct tgtcgtcggt ccattcggcc tggtggcagg cgcccgtcga gcaggattca
     2761 aacgtgccgc ggtccaccgt atcggtgatg gcgccggtca gttccaggtt ccagtcggcg
     2821 gtcagcggtt tgtagctgat ggcgctgacc cacttgatgg cccagtgacc atccacaatc
     2881 tggttgccct ccgggctgat gaccatcagg cgcagggcgc cttcttcttc ggcgttcagc
     2941 ggtgcgccgg cgcgttcata agccagcagc acctgcagcg gacccggggc ggcaatttca
     3001 tcgccggtgc cggggtcata ggtgataaat tcgccgttgg caacctggtc gggcgagaag
     3061 gtcatggcgt agccatcttc tgccgtaatc tggatcgcct ggtcagactt cagcccatcc
     3121 acctcatcca gcaggtcggt gagcagcacg ccgcggtaca gctctggcac ggtgattttg
     3181 cccgtgctgc tcttggtacc ggcatagccc tcggtga
//
LOCUS       DAOPVS010000191         3214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_30697_length_3214_cov_46.264957, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000191 DAOPVS010000000
VERSION     DAOPVS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30697_length_3214_cov_46.264957"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKL11_10410"
     CDS             complement(<1..517)
                     /locus_tag="PKL11_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNZ14052.1"
                     /translation="MRRVLVAYSTNSGSTAEVAAAVAAELTAAGHAADVQPLANVTAL
                     DGYDAVVVGAPMIFGWQPAARRFLQRHQKTLAAKKVACFACAMRLTRAPGESLPPVPL
                     LLDPNLVSEPAKLGALNLKERFTTIGHYLKSMLGGAPAVQPLSVAFFGGRLEMYRLKW
                     WQAAFVMIVVQA"
     gene            complement(527..967)
                     /locus_tag="PKL11_10415"
     CDS             complement(527..967)
                     /locus_tag="PKL11_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNZ14053.1"
                     /translation="MDAKIQAFIQAKRLAVVGVSRSAQKFGTAIYTELKARGFEVYGV
                     NPQMETIAGDKCYATIAELAGKVDGVIICVQPQKAAGAIREAAAAGIQQIWLQQGSQS
                     LEATQAAREAGVSPVEGKCILMYAGEVKSIHGFHRFFARLFGQY"
     gene            complement(1104..2423)
                     /locus_tag="PKL11_10420"
     CDS             complement(1104..2423)
                     /locus_tag="PKL11_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14054.1"
                     /translation="MNEQEFKHRLLHHLAETRVPARDHPWDAVEKRLHARRQARRTLR
                     LAFAAGCVLAGVIFFLTPLGQAAAQSILSLFTRAESDVRPVPTDQQSAALVPSPTPVP
                     TQMVALQAITPGSTFWTPTPAPTRESNGSLWGLTLSEAAALAQFEPLAPAEVLAGYSL
                     ADVVYNPDPATVTQIYKFTPYQAGEMFILSQRAALPDDAVGQSAEVETFWVGDVLVET
                     VRGGWFAPAGAAVEQWENDIPVHAYRWQQNGYTFTLDFWVGDTFSPAYLTAVDRQAQI
                     EVLLGARAALPERMNLNNLESIEAVRAVSEQPVLAPTLLPEGFIFGHGVYETGRVVLI
                     YEPAAGTRYSGNASLTIFETPLNPGAAPLTYDNYPPGAVETVAIGPYSGTLVRGQMMD
                     NVFDPDAGASLVWQTETLNINLWVRYSSTYAARLAPEDLITLAGSME"
     gene            complement(2428..3036)
                     /locus_tag="PKL11_10425"
     CDS             complement(2428..3036)
                     /locus_tag="PKL11_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ14055.1"
                     /translation="MEEQHAIALLKAHDLTGLEALVDRYYLQAVRAADLIVRDTAQAE
                     DIVQAAFLHASEKIDQLASDRFGPWFMKMVVNAALKSAQKQQRLVPLTAEDDPESQPL
                     ADWLIDRRPAVEELVETAELRQSIWQALARLTPRQRAAVVLKYYLDMSEVEISQALQA
                     PRPTIKWWLYTAREKLRHWLRLTDAPHAPFEPESVSDHQEKE"
BASE COUNT          611 a          990 c         1002 g          611 t
ORIGIN      
        1 cggcctggac aacgatcatc acaaaagccg cctgccacca tttcaggcgg tacatctcca
       61 gccgcccgcc aaaaaaggcc acactgagcg gttgaacggc tggcgcgccg cccagcatcg
      121 atttgaggta gtgcccgatg gtggtgaagc gctctttgag gttcagcgcg ccaagtttgg
      181 ctggttcaga taccaggttg gggtccagca gcagcgggac gggcggcagg gactcgccgg
      241 gcgcgcgggt caggcgcatg gcacaggcaa agcaggccac ctttttggcc gccagtgttt
      301 tttgatgacg ctggaggaag cgccgcgccg ccggctgcca gccgaagatc atcggcgcgc
      361 ccaccaccac agcatcgtaa ccatccagcg cggtcacgtt tgccagcggc tgcacgtctg
      421 ccgcatgccc ggcagcggtc agctcggcgg caacggcggc ggctacctcg gcggtggagc
      481 ccgagttggt cgaataggct accagcaccc tgcgcatcgg gcgcactcag tactgaccga
      541 agagtctggc aaagaagcgg tggaagccgt gaatcgactt gacctcaccg gcgtacatca
      601 aaatacattt gccttccacc ggcgagacgc ccgcctcgcg cgcggcctgg gtggcctcga
      661 gcgactgcga accctgctgc agccagatct gctggatgcc ggcggcggca gcctcgcgga
      721 ttgccccggc agccttctgc ggctgcacac agatgatcac gccatccacc ttgcccgcca
      781 gctcggctat ggtggcgtag catttatccc cggcaattgt ttccatctgg ggattgacgc
      841 cgtacacctc gaagccgcgg gctttcaatt cggtgtagat ggccgtgccg aatttctgcg
      901 ccgagcgtga gacccctaca accgccaatc gtttggcctg aataaaggct tgaatttttg
      961 cgtccatcag aacttcctga aaattgagaa aaatgggaat gggcctatct tacacccata
     1021 cgcggttttt gtgggcctca gataatattt gaatcaaaac tgcccggaga agtctccggg
     1081 cagcgggggt ggaaacgccg gggctattcc atgctcccgg ccagggtgat gagatcttcc
     1141 ggcgccagcc gggcggcata agttgatgaa tatctgaccc agaggttgat attcagggtc
     1201 tcggtctgcc agacgagcga ggcgccagca tccgggtcaa agacattgtc catcatttga
     1261 ccgcgcacca gcgtgccgct gtaggggcca atggcaaccg tctccacggc gccgggcggg
     1321 taattgtcat aggtcagcgg ggcggcgccg gggttgaggg gcgtctcaaa gatggtcagg
     1381 ctggcattgc ccgagtaccg cgttccggcg gcaggttcgt agatcagcac cacccgcccg
     1441 gtttcgtaca ccccgtgccc gaagatgaag ccctctggca gcagggtggg cgccagcact
     1501 ggctgctcgc tgacggcgcg cacggcttcg atgctttcca ggttgttgag gttcatccgc
     1561 tcgggcaggg ccgcgcgggc acccagcagc acctcaatct gggcctgcct gtccacagcc
     1621 gtcaggtaag ccgggctgaa tgtatcgcca acccagaagt ccagcgtgaa ggtgtaacca
     1681 ttctgctgcc agcggtaggc gtggacgggg atatcgttct cccactgttc caccgcggcg
     1741 cccgccgggg caaaccagcc gccgcgcacc gtctcgacca gcacatcgcc aacccagaag
     1801 gtctctacct cggcgctctg cccaacggca tcatcgggca gggccgcgcg ctgtgagagg
     1861 atgaacatct cacccgcctg gtagggcgtg aatttataaa tttgcgtcac ggtagccggg
     1921 tcggggttgt aaaccacatc cgccaggctg tagccagcca gcacctcggc gggcgccagc
     1981 ggctcaaact gcgccagcgc cgctgcctcg ctgagggtca aaccccacag gctgccattg
     2041 gactcgcggg tgggggcagg ggtgggtgtc caaaaagtgc tgcccggcgt gatggcttgc
     2101 agcgccacca tttgcgtggg caccggggtg gggctgggca ccagcgccgc agactgttga
     2161 tcggtgggca ccgggcgcac atcgctctcg gcgcgggtga agagtgacag gatgctctgc
     2221 gcggccgcct gtcccagcgg cgtcaaaaag aagatcaccc cggcaagcac acagcctgcg
     2281 gcaaaggcca gccgcagggt gcggcgggct tggcggcgcg cgtgcaagcg cttttccacg
     2341 gcatcccagg gatgatcacg ggcggggacg cgcgtttctg ccaggtgatg cagcaggcgg
     2401 tgtttgaatt cttgttcgtt catggcctta ttccttttcc tgatgatccg aaaccgattc
     2461 gggctcgaaa ggcgcgtgag gcgcatcggt cagccgcagc cagtgacgca gtttttcccg
     2521 ggcagtgtac agccaccatt tgatggtggg gcgcggcgcc tgcagcgcct gactgatctc
     2581 gacctcgctc atgtccaggt agtatttcag caccaccgcc gctcgctgcc ggggcgtcag
     2641 ccgcgccagc gcctgccaga tgctctgccg cagttcggca gtctccacca gttcttccac
     2701 ggcagggcgc cggtcaatca accagtctgc caggggctgg ctttcggggt catcttcagc
     2761 cgtcagcggg accaggcgct gctgtttttg ggcactcttg agcgccgcat tgaccaccat
     2821 cttcataaac caggggccaa agcggtccga ggctaactgg tcaatctttt cgctggcgtg
     2881 caggaaagcc gcctggacga tatcttccgc ctgggctgta tcccggacga tcaggtcagc
     2941 ggcgcgcacg gcttgcaggt agtaacgatc caccagggct tccagcccgg tcaggtcgtg
     3001 cgctttgagc agcgcaatgg cgtgttgttc ttccatgcag ttcctttcga ggtgcaactg
     3061 aggatcctct acttatatga tccttcaggc tcgaaaaaag gcgggaaaag gccggctctc
     3121 aatttgatta taaaaccttt aaccaggcac aatttaccga aaactgctcc caaaatattc
     3181 aaagtatgat aaacttgccc ttaatcaaca gtag
//
LOCUS       DAOPVS010000192         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_30758_length_3210_cov_51.912520, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000192 DAOPVS010000000
VERSION     DAOPVS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30758_length_3210_cov_51.912520"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKL11_10430"
     CDS             <1..566
                     /locus_tag="PKL11_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNZ14056.1"
                     /translation="AWASGKVSTGGLLRNWAIVYLGNFVGSVLIALLVLWGKHYTAGG
                     GSMGLAMLSIANAKLHLGFWQAIALGVLCNLLVCLAVWLTYGARSVSDKILAIIFPIA
                     AFVAAGFEHSVANMYFIPLGLFLKEIDPAFVSNTAAQAGLDLTGMTWGNFLLKNLLPV
                     TIGNIIGGAVMVGLIYWVVYLYKRQSS"
     gene            672..1175
                     /locus_tag="PKL11_10435"
     CDS             672..1175
                     /locus_tag="PKL11_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ14057.1"
                     /translation="MVTKTNLVSTLQNIPWLLELKAPQLERLAALAQIQHLDENEVLY
                     NEGERVGCLYILLEGQISIENNIPGSGAIRILVAEALEIVGWSSMTLLVRLRNNSARA
                     ITAATLLCFDDEGLRALCEEDHHIGYLFMRRISNLVARYLLTTRLQLMEKLVEQSERH
                     LPTIGGE"
     gene            1236..1580
                     /gene="hypA"
                     /locus_tag="PKL11_10440"
     CDS             1236..1580
                     /gene="hypA"
                     /locus_tag="PKL11_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNZ14058.1"
                     /translation="MHELSVTESILNIASRHAEQAEAKRVTDIHIVVGKLSSIVDDSI
                     QFYWDMISEDTICAGATLHFQRVPARLACLDCNAEFELSAELIPCPKCGSVRVKVLAG
                     EEFWLDSIEITR"
     gene            1591..2262
                     /gene="hypB"
                     /locus_tag="PKL11_10445"
     CDS             1591..2262
                     /gene="hypB"
                     /locus_tag="PKL11_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNZ14059.1"
                     /translation="MSSIRVPVIEKIFNANDQLAVLNRKRLDEAGVFAINLMASPGAG
                     KTTLILNTIRALAPEIRIGVIEGDTAPVTIDADKVLAAGMPAVQINTGGDCHLDAPMI
                     EQGLNSLPLKELDLIIIENVGNLICPAAFQLGSHANVLIASVPEGDDKPYKYPNIYRG
                     VQVLVVTKTDLLPYVTFNMDYFRQGVEMLSPGLVTFPLSCKTGEGVAEWTNWVRQQVK
                     QKRGQ"
     gene            2259..>3210
                     /locus_tag="PKL11_10450"
     CDS             2259..>3210
                     /locus_tag="PKL11_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNZ14060.1"
                     /translation="MSPEIVGLKIRVGGIVQGVGFRPFVFGLAEQCKVTGWVRNTSSG
                     VEIEINGTTEAVSQFAETLRTSPPPLARIDRFETERISPNGAVDFQILVSQPQEGEFL
                     PISPDMTICPDCQRELFDPQDRRFRYPFINCTNCGPRFTIVKDIPYDRPKTTMADFPL
                     CPTCAGEYHDPRDRRFHAQPVACPDCGPRVWIEIDGRCAEEQEAAIQAARTRLKAGRI
                     MAIKGLGGYHLACDATNQQAVAELRQRKKRSDKPFALMAFDLAAVERHCYVSAEERSL
                     LTSRQRPIVLLERRAESLIAQEAAPGQTTLGFMLAYTPLHL"
BASE COUNT          735 a          781 c          955 g          739 t
ORIGIN      
        1 tcgcctgggc cagtgggaag gtgagcaccg gcggattgct gcgcaactgg gcgattgtgt
       61 acctgggtaa ttttgtggga tccgtcttga ttgcgctgct ggtgctgtgg ggaaagcatt
      121 acaccgcggg cggcggaagc atggggctag ccatgctgtc gattgccaac gccaagctgc
      181 acctggggtt ctggcaggcg attgccctgg gtgtattgtg taatctgctg gtttgcctgg
      241 cggtctggct gacgtatggc gctcgcagtg tgtccgataa aattctggcg attatattcc
      301 ccattgcggc ctttgtggcg gcaggatttg agcacagcgt tgccaatatg tatttcattc
      361 ccctggggtt attcctgaaa gaaatagatc cggcgtttgt gagcaataca gccgcccaag
      421 ccgggctgga cctgaccggg atgacgtggg ggaattttct gctcaagaac ttgctgcccg
      481 tgacgattgg gaatatcatc ggcggagctg tgatggttgg gctgatttac tgggtggttt
      541 atctgtacaa aaggcagtcg tcataggtga caatcgcccc ctgccgcggt gacagagtac
      601 acttcatcgg gatttaattc aatgatacaa agagagtgta ctcgctcgac tatatgattg
      661 gatcaaactt catggtcacc aaaaccaatc tggtttcaac cctccaaaac attccctggc
      721 tattagagct caaagcgccg cagcttgaac ggctggcagc gctggctcag attcagcacc
      781 ttgacgagaa tgaagtgcta tataatgaag gggaacgtgt ggggtgcctg tatattttgc
      841 tggaagggca aatctctatc gagaataata ttcccgggag cggggcaatc cgtattctgg
      901 tggctgaggc gttggagatt gtgggctggt ccagcatgac actcctggtg cggctgcgaa
      961 ataacagcgc ccgggccatt acggctgcaa cgctactgtg ctttgacgat gaaggtctgc
     1021 gcgcactgtg tgaagaagat catcacattg ggtatttgtt tatgcgccgg atcagcaatc
     1081 tggtggcccg gtatctgctg accacccggc tgcaacttat ggaaaaactg gtcgaacaat
     1141 cagaacgcca tctgccaacg attggcggcg agtgaaattc gcaccagatc agtcaagatt
     1201 gacttaattt atcttagaag tataattacg ccactatgca tgaactttcg gttactgaaa
     1261 gcatcttgaa tattgcttca cgccatgctg aacaggcaga ggcgaaaagg gttacagaca
     1321 ttcacattgt tgtgggaaag ctctcgagca ttgtggatga ctcgattcag ttctattggg
     1381 atatgatcag cgaggacacc atctgtgcgg gcgccacact gcactttcag cgtgtgccgg
     1441 cgcggttagc ctgcctggat tgtaatgcgg aatttgagct gagcgctgaa ttgatcccct
     1501 gccccaaatg cggcagcgtg cgcgtaaaag tactggctgg cgaggaattc tggctagaca
     1561 gcattgaaat cacccgttaa ggagccttga atgtcttcaa ttcgcgtacc tgtgattgaa
     1621 aaaatattta atgccaatga tcaactggcg gtcttaaacc ggaaacgact ggacgaggcc
     1681 ggtgtttttg cgattaattt gatggcatcg ccaggggcag gaaaaaccac gttgattttg
     1741 aacaccatcc gtgccctggc acctgagatt cgcatcggtg tgatagaagg cgacacggcg
     1801 ccggttacga ttgatgccga caaggtgctg gcggcaggta tgcctgcggt gcaaataaac
     1861 acgggcggag actgccacct tgatgcgccc atgattgaac aagggctcaa cagcctgccg
     1921 ttaaaagagc ttgacctgat tattattgaa aacgttggca acctgatttg cccggcagca
     1981 tttcaactgg gctcgcatgc caatgtgctg attgccagcg tgccagaagg cgatgacaaa
     2041 ccctacaaat accccaatat ttaccggggc gtgcaggtgc tggtggtgac caaaaccgat
     2101 ttgctgcctt atgtgacctt caatatggat tatttccggc agggcgtcga aatgctcagt
     2161 ccagggctgg tcaccttccc gctctcgtgc aagaccgggg aaggcgttgc cgagtggacg
     2221 aactgggtgc gccagcaggt aaagcaaaag cgggggcaat gagccctgag attgttgggc
     2281 tgaagattcg agttggcgga atcgtgcagg gggttggatt tcgccccttt gtctttggac
     2341 tggcggagca atgcaaggta acgggctggg tgcgaaatac atccagtggc gttgagattg
     2401 agatcaacgg aacgacagaa gcggtcagcc agtttgcaga aacgctgcgg acttcaccgc
     2461 caccgctggc ccggattgac cgttttgaga ccgagaggat ttcacccaat ggcgcagtgg
     2521 attttcaaat tctggtcagc cagccgcagg aaggcgaatt cttgcccatc tcgccggata
     2581 tgaccatctg cccggactgc cagcgggagt tgtttgaccc gcaagaccgg cggttcaggt
     2641 atccatttat caactgcacc aactgcgggc cgcgctttac cattgttaaa gacattccgt
     2701 atgaccgccc taaaacgacc atggcggatt ttccgttatg cccgacctgt gcgggagaat
     2761 atcacgaccc gcgcgaccgg cgatttcatg ctcagccggt ggcctgcccg gattgcgggc
     2821 cgcgggtttg gattgaaatt gacggacggt gcgccgaaga acaggaggcc gccattcaag
     2881 ccgcccgcac taggctgaaa gccgggagaa taatggcgat aaagggattg gggggatatc
     2941 acctggcgtg tgacgccacg aaccaacagg ccgtggctga gctgcggcag cgtaaaaaac
     3001 ggagcgacaa accgtttgcg ttgatggcgt ttgacctggc ggcagttgag cggcattgtt
     3061 atgtgtcagc agaggagcgc agcctgctga cctcccggca gcggcctatt gtgttgctgg
     3121 agcgcagggc agaatcgctg attgcgcagg aagccgcacc cggacaaaca accctgggat
     3181 ttatgctggc ctacaccccg ctgcacttgc
//
LOCUS       DAOPVS010000193         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_31241_length_3174_cov_58.033665, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000193 DAOPVS010000000
VERSION     DAOPVS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31241_length_3174_cov_58.033665"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1086
                     /locus_tag="PKL11_10455"
     CDS             253..1086
                     /locus_tag="PKL11_10455"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNZ14061.1"
                     /translation="MSQTNTPHFLLAHKLAGYFARLPQVEAIALGGSQTSGSPDATSD
                     IDLYVYTRGAEIPLSVRQSILAQSGGASTANLGLTYWGPGDEWVDAATGIEVDLVYFD
                     ADWMQQQINRVILDHQPSLGYTTCFWHTCRHSQSLHDPQGWFASLQTLSQQPYPEPLR
                     TNIITYNHPVLRQIIPSYFNQIEKAVNRQDLVSLNHRLAALFTSYFDILFAVNRLLHP
                     GEKRLLHFARSRCRKLPVDFDSDMEQVMTASARPQTDLLLHLTRLLDHLDALLEAEKF
                     M"
     gene            1098..>1430
                     /locus_tag="PKL11_10460"
     CDS             1098..>1430
                     /locus_tag="PKL11_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14062.1"
                     /translation="MELKRELGTPIALGRTAEIYAWQDNQVLKLFYDWFPLESIEYEA
                     QIARSISATGLPVPTVGDIIQINQRNGLLYERLNGESMFAALIHRPWNVLRYGRHLAE
                     LQADMHARS"
     assembly_gap    1431..1530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1636..3126
                     /locus_tag="PKL11_10465"
     CDS             1636..3126
                     /locus_tag="PKL11_10465"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNZ14063.1"
                     /translation="MNHRPRIQSLALIFLLLLFLSACSLSEPTAQPPVPTREVSDIPP
                     AVNSPGSDSLGDSLYAGFGNGGYDVQHYTLDLTITDVEAGEMDGIVTINARAVQDLDA
                     FNLDFIGYEISAVTVDSNPADFTRADQELTIIPARPISSGTAFTVGISYSGTPERLES
                     QAAVGRVGWIPLDDGSLVLSEPDGAATYYPVNDHPLDKAAYTFQITVPSDYEVVANGV
                     LSDTSDNGDSVTYVWQATDPMASYLTTVNIGDYDLETDESDSGVPIRNYFAATLDDDA
                     REPFARQAEMIDYFSEIFGPYPFEVYGSIVIDAEVGTALEAQTLSLYGIDQLDLDDID
                     SAEQLVAHELAHQWFGDSISVADWGDIWLNEGFATYSEGLWIEYTQGAEARDEWVMDN
                     YEYVADYLQDEAPPGTPAADNLFSERIYCQGALTLHALRLQVGDDTFFKIIQTYFDRY
                     QGGNARTADFIAVAEEISGQDLTEFFDSWLYADTIPAIPELDLSSD"
BASE COUNT          661 a          919 c          739 g          755 t
ORIGIN      
        1 caatccggtg cagtcggacc ctgactgcac cggatttatg ttattcataa aaattcagta
       61 agcccgtcct ttctgcaatg ccactgttgt gtgcctgcag attgatatta cgctgcaatc
      121 ctgtgataat cccaagaaaa agctgaaaaa tacctgagaa accagccttc agtggaaaag
      181 tgtggctccc acagtcaaaa tgcgccccgg tttatgatat gataaattat ccttcttccc
      241 gcatagtttc taatgtctca aacaaacact ccccacttcc tgctcgcaca caaactggca
      301 ggttattttg ccagacttcc ccaggttgag gcgattgccc tgggcggttc gcaaaccagc
      361 ggctcgccag atgcaacatc tgatattgac ctctatgtct acacccgcgg cgcagaaatt
      421 cccctctctg tccgccaatc gatcctggcg cagtctggcg gcgcctccac agccaacctg
      481 ggcctcacct actggggtcc gggtgatgaa tgggttgatg ccgctaccgg catcgaggtg
      541 gacctggttt attttgatgc cgattggatg cagcagcaaa tcaaccgggt aattctcgac
      601 catcagccca gcctgggcta caccacttgc ttctggcaca cctgccgcca ctcacagagc
      661 cttcacgatc cgcagggctg gtttgcatca cttcaaaccc tcagccagca gccttaccca
      721 gagcctttgc gtaccaacat tatcacctac aatcatccgg tcttgcgcca aattatccct
      781 tcctacttca accagattga aaaagcagtc aaccgtcagg accttgtcag tctcaaccat
      841 cgcctggctg cactcttcac cagctatttt gatattctct ttgctgtcaa tcgactcttg
      901 catcccggcg aaaagcgcct gctccacttt gcccggtcgc gctgccgcaa actcccggtt
      961 gattttgatt cggatatgga gcaggtcatg accgcctccg cgcgccctca aaccgatctc
     1021 ctcctgcacc tcacccgcct gctggatcac ctggatgcgc ttctggaagc cgagaagttc
     1081 atgtgaactg aaagtaaatg gaactgaaac gtgagcttgg tacccccatc gcccttggcc
     1141 gcaccgccga aatttatgcc tggcaagata atcaggtgct taagttattt tatgattggt
     1201 tccccctgga atctattgag tatgaggcgc agattgcccg ctcaatttca gctacagggc
     1261 tgcctgtgcc caccgtgggg gatatcatcc agattaatca gcgcaatgga ttgttgtatg
     1321 aacgtttgaa tggcgaatcg atgtttgcgg cacttattca tcggccctgg aacgtgctcc
     1381 gctacggccg ccaccttgcc gaacttcagg ccgatatgca tgctcgctca nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgttggctgc ccgtcgttgc tgctgctcgc
     1561 ctcagtgaag atattattga attgcaaccc tggctaattg ctcaggttgc gtctcgttat
     1621 gctgaaaggt aaaaaatgaa ccatcgcccc cgtattcaat cgcttgcctt gatcttcctg
     1681 ctcttgttat ttctatctgc gtgcagctta tctgagccaa cggctcaacc tcccgtacca
     1741 acccgtgagg tctccgatat cccccccgcc gtcaattcgc ccggctctga cagccttggt
     1801 gattctttgt atgctggctt cggcaacggc gggtatgatg tccagcatta caccctcgac
     1861 ctcaccatca cagatgttga agctggcgaa atggatggca ttgttacgat taatgcccgg
     1921 gctgtccagg atctggacgc cttcaacctg gattttatcg gttatgaaat cagcgccgtc
     1981 accgtcgatt caaacccggc tgacttcact cgtgcagacc aggaactgac cattatccct
     2041 gcccggccga tttcttccgg cactgctttt acagtcggca tctcctactc aggcactcct
     2101 gagcggctcg agtcccaggc cgcggttggg cgggtagggt ggatccctct tgacgacggc
     2161 agtctggtgc tcagcgaacc cgatggcgcc gcgacttact atccggtcaa cgaccatcca
     2221 ctcgataagg cggcttacac cttccaaatc accgtgccat cggattatga agttgttgcc
     2281 aacggcgtcc tgtctgatac ttcagacaac ggggactcag tgacctatgt ttggcaagcc
     2341 accgatccca tggcaagtta cctgacaact gtcaatatcg gtgactatga ccttgagacc
     2401 gacgaatccg attccggcgt tcccattcgt aactactttg ctgctaccct ggatgacgac
     2461 gcccgcgaac cctttgcccg gcaggctgag atgatcgact atttcagcga aatctttggc
     2521 ccctacccgt ttgaggtcta tggctccatc gtcattgacg ccgaagtcgg cactgccctg
     2581 gaggcgcaaa cgctgtctct gtacggcatt gatcagcttg acctggatga tatcgactcg
     2641 gctgagcagt tggtcgccca cgagttagcc caccagtggt tcggcgacag catctctgtc
     2701 gctgactggg gggatatctg gctgaatgag ggcttcgcca cctattctga aggcctgtgg
     2761 attgagtaca cccagggcgc cgaagcccgc gatgagtggg tgatggacaa ctatgaatac
     2821 gtcgccgact atctgcaaga cgaagccccg cccggcacac ccgcggcaga taacctgttc
     2881 tccgaaagga tttattgcca gggcgcctta accctccatg cgctgcgctt gcaggttggc
     2941 gatgatacct tcttcaaaat catccaaacc tactttgacc gttaccaggg cggcaacgcc
     3001 agaaccgccg actttatcgc cgtagccgag gaaatcagcg gccaggattt gaccgaattt
     3061 ttcgatagct ggttgtatgc cgacaccatc cccgcgatcc ccgagttgga cctgagttca
     3121 gattaatcgg cgctttgcca gcagggagat acctttgacc gtaagatttg gcat
//
LOCUS       DAOPVS010000194         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_31242_length_3174_cov_47.844181, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000194 DAOPVS010000000
VERSION     DAOPVS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31242_length_3174_cov_47.844181"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1434
                     /locus_tag="PKL11_10470"
     CDS             190..1434
                     /locus_tag="PKL11_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNZ14064.1"
                     /translation="MMNRPIEPGLLKIFKYFTSIAMAYFAILWAYAILYAGWEWSLQL
                     QLWLNFAVYLGLAGYLAWGELEQRLKQFYLPIGLAISTLFPVLNNFVYLMGPYQANID
                     IIISRSWLWLPVLLVPLVLIAWQYNLKSVIGFTVFTNGLELIVLLVVVKTINFDTIAL
                     LGVPLVRAFAFGIVGYVIGNLMETQRNQQQKLVQANVRLGQYASTLDQLATSRERNRL
                     ASELHDTLAHTLSGLAITLEAIKTVLDPQQTEATEMLEHSLQATRVGLDETRRALKAL
                     RARPLEDMGLKLALEKVSQAAAERAAIPIDFIYPAELPALPTDVEQCMYRVTQEALEN
                     MIRHAGATQAAVEVRSNQGRIELIIRDNGIGFEPSDKKLEDRFGLFGLHERAKSVAGQ
                     LEVHSQPGQGTQIKFTWEKFND"
     gene            1427..2077
                     /locus_tag="PKL11_10475"
     CDS             1427..2077
                     /locus_tag="PKL11_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ14065.1"
                     /translation="MIRVLICDDQEIVCEGLQRILDTDGEINVVGTVHNGLEALEMVE
                     RLQPDLVLMDLKMSVMNGVVATRKIRERYPEIRVLVLTTYDDDEWLFDAIRSGAAGYL
                     LKDRPREELINAIKGTMAGKTYIDPSVAGKVLINVAQNMPRQQPPVEISLSEREQEVL
                     QLMTQGLSNADIAGRLFLSEGTVRNYTSAIFSKLNVSDRTQAVILALRYGLVDLNR"
     gene            complement(2050..>3174)
                     /locus_tag="PKL11_10480"
     CDS             complement(2050..>3174)
                     /locus_tag="PKL11_10480"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HNZ14066.1"
                     /translation="ADQGGIRFYNLDNEPMLWSSTHRDVHPNPASYDEMRDRTWAYAS
                     AIKSADPSALTLGPVTWGWTAYFWSALDWADPDFPSVTPDRTAHGGLPFTAWYLQQMQ
                     AYEQANGIRILDYLDLHYYPQANGVSLSPAGNAATQALRLRSTRALWDETYADESWIA
                     DVPEGPYVRLIPRMHAWVETYYPGTKLAITEYNWGALDHINGALAQADVLGIFGREGV
                     DLAALWGPPESNQPGAFAFRMYLNYDGYHHGFGETSIQAVSTDQDRLAVYAAQRGMDA
                     ALTLMIINKTGADLVSPVALTNFAPASSAQVYRYSAQDLTRIIRQPDQPVSSTGFTAT
                     YPANTITLVVIPPLIPPQQTYLPTVLKGLPRSQRFKSTNP"
BASE COUNT          724 a          730 c          999 g          721 t
ORIGIN      
        1 tcaaagattc ggcttgtata gcgatatcca tgcggttatc tcctgagata ttgctctatc
       61 actataaggc attgcaataa ccgtggcgag atgcatgaca gcatgaaccg gtatgacggg
      121 cgccacgcgt tggaatgatg ggcgtcatca acttgtatga ggtcgtttta aatcgtgtta
      181 tgataaatca tgatgaaccg cccgattgaa cccggactgc tgaaaatatt caagtatttc
      241 accagcatag caatggctta ttttgccatt ctgtgggcat atgcgattct ttatgccggg
      301 tgggagtggt cgctgcagct tcaactctgg ttgaattttg cggtttatct ggggctggcg
      361 ggttatctgg cgtggggcga gcttgagcaa cggctcaagc agttttatct gcccattggg
      421 ctggcgatca gcacgctttt tccggtgtta aacaattttg tttatctcat ggggccctat
      481 caggccaaca ttgatatcat catcagccgc agttggctgt ggctgccggt gctgctggtg
      541 ccgctggtat taattgcctg gcaatataac ctcaagtcgg taattgggtt cacggttttc
      601 accaatgggt tggaattgat tgtgctgttg gtggtggtta aaaccatcaa ttttgatacg
      661 attgcgctgc tgggggtgcc cctggtgcgc gcctttgctt ttggaattgt gggctatgtg
      721 attggaaacc tgatggagac ccagcgcaat caacagcaaa aactggttca agccaatgtg
      781 cggctggggc aatatgccag cacattagat caactggcga ccagccggga acgtaaccga
      841 ttggcaagcg aactgcatga tacgctggcg catactttga gcggactggc gattaccctg
      901 gaggcgatca aaacagtttt ggatccccag cagaccgaag cgaccgagat gctggaacac
      961 tcgctgcagg ccacccgggt ggggctggat gaaacccgcc gcgccttgaa agccctgcgg
     1021 gcgcgcccgc ttgaagatat ggggctgaaa ctggcgctgg aaaaggtcag ccaggcggcg
     1081 gcagaacggg cagccatccc gattgacttt atctacccgg cagagctgcc ggccctgcca
     1141 accgatgtgg agcagtgcat gtatcgggtg acgcaggaag cgcttgaaaa tatgatccgg
     1201 cacgctggcg ccacgcaggc cgccgttgaa gtgcggagca atcagggacg gattgagctc
     1261 atcatccggg ataacggcat tgggtttgaa ccgtctgata aaaagcttga agaccggttt
     1321 ggattatttg gcctgcacga gcgggcaaaa tctgttgccg ggcagcttga agttcacagc
     1381 caacccggtc aaggcacaca aattaagttc acctgggaga agtttaatga ttagagtttt
     1441 gatttgtgac gaccaggaaa ttgtctgcga agggctgcag cggatccttg atacggacgg
     1501 tgaaatcaac gtggtaggga cagtgcataa cgggctggaa gcgctggaga tggttgaaag
     1561 gctgcagccg gacctggttt tgatggattt gaagatgtct gtgatgaatg gtgtggtggc
     1621 aacacgtaag attcgggagc gttatccgga gatccgcgtg ctggtactga ccacttatga
     1681 tgacgacgaa tggctgtttg atgccatccg cagcggtgcg gcgggctatt tgctcaaaga
     1741 ccggccgcgc gaagaactga tcaacgcaat caaaggaacg atggcaggga aaacttacat
     1801 tgaccccagc gttgctggaa aggtgctcat taatgtggct caaaatatgc ccaggcagca
     1861 gccgccagta gaaatctcgc tgagtgagcg ggagcaggaa gtactgcaat tgatgacgca
     1921 ggggctgtcg aatgcggata ttgccgggcg attgtttttg tctgaaggca cggtgcggaa
     1981 ttacaccagt gcgatcttca gtaagctcaa tgtatctgat agaacgcagg cggtgattct
     2041 ggcgctgcgc tatgggttgg tggatttaaa ccgttgagaa cggggcaggc ctttgagaac
     2101 tgttggcagg taggtttgct gcggggggat taagggcggg atcacgacca gggtgatggt
     2161 gtttgccgga taggtggcgg taaaaccggt tgaggaaaca ggctggtcgg gttgacggat
     2221 gatgcgggtt aaatcctgtg cgctgtagcg gtacacctgt gcgctggatg ccggtgcaaa
     2281 attggtgagt gccaccgggc tgaccagatc ggcgccggtt ttgttgatga tcatcaatgt
     2341 cagggcggca tccatgccgc gctgcgccgc ataaacagcc agccgatctt gatctgtgct
     2401 gacggcctgg atgctggttt cgccaaagcc atgatgatag ccatcgtaat tcagatacat
     2461 tctaaaggca aaggcaccgg gttgattgct ttctggcggg ccccagagag cggcgagatc
     2521 cacgccttcg cggccaaaga tgcccagaac atctgcctga gccagagcgc cgttgatatg
     2581 atccagggcg ccccagttat attcagtaat ggcgagcttt gtgccaggat aataggtctc
     2641 aacccacgca tgcatccgtg gaatcaggcg gacgtaggga ccttcgggga catcggcaat
     2701 ccaggattca tccgcatagg tttcatccca cagcgcccgg gtggagcgca gccgcaaagc
     2761 ctgggtggcg gcattgccag ccggtgaaag cgaaacgccg ttggcctggg gataatagtg
     2821 caaatcgaga taatccagga tgcgaatgcc gttggcctgt tcataggcct gcatttgttg
     2881 aaggtaccag gccgtgaaag gcaggccgcc atgggcggtg cggtctggtg tgacgctggg
     2941 gaagtcggga tcggcccagt cgagggctga ccagaagtat gctgtccagc cccaggtgac
     3001 ggggccaagc gtcagcgcag atggatcagc cgatttgatg gcagaggcat aagcccaggt
     3061 gcggtcacgc atttcatcat aagaagcagg attggggtgg acatctcgat gtgtgctgga
     3121 ccagagcatg ggctcgttat ccagattgta gaaacggatg ccgccctgat cggc
//
LOCUS       DAOPVS010000195         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_31496_length_3158_cov_57.007734, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000195 DAOPVS010000000
VERSION     DAOPVS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31496_length_3158_cov_57.007734"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="cydB"
                     /locus_tag="PKL11_10485"
     CDS             complement(<1..560)
                     /gene="cydB"
                     /locus_tag="PKL11_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNZ14067.1"
                     /translation="MDLNIVWFILIAVLFIGYFVLEGFDMGVGILLPFIAKTDIRRRV
                     VINTIGPHWDGNEVWLLTAGGAMFAAFPHWYATVFSGFYLPLFLILIGLILRGAALEF
                     RSKDENPKWRSLWDWAIFVGSLLPALLWGVAFADFILGVPVDASKIFVGTFFDLVPPL
                     ALLGGVVSLLMITFHGAIFLSLKTTEP"
     gene            complement(585..>1255)
                     /locus_tag="PKL11_10490"
     CDS             complement(585..>1255)
                     /locus_tag="PKL11_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNZ14068.1"
                     /translation="GSLGVAVIGHAQGQNTVRTQPMKMAAAEALWHTEDPAAFSLVAG
                     IDENAQQNTFALRIPGMLSFLSYNRFSGEVKGMLDLQKEFEAKHGPGNYIPPVAMTFW
                     TFRLMVGIGTLMIVLALYLVFMIWRKKPVPAKLLPIFPFAIALPFLANTFGWLLTEMG
                     RQPWVVYDQLTLSQAVSPNLTSGMVLTSLIGFTLIYALLMAADVYLLAKYAKTVPAHH
                     TARH"
     assembly_gap    1256..1355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1356..1762)
                     /locus_tag="PKL11_10495"
     CDS             complement(<1356..1762)
                     /locus_tag="PKL11_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNZ14069.1"
                     /translation="MDELTLILSRWQFAITTIYHFFFVPLTLGLGLLVAILETRYVRT
                     GQEAHKRQTQFWGKLFVINFAMGVVTGIVQEFQFGMNWSEYSRYVGDIFGAPLAIEAL
                     LAFFIESTFLGLWIFGWEKLSKKMHLAAIWLVS"
     gene            complement(1871..3091)
                     /locus_tag="PKL11_10500"
     CDS             complement(1871..3091)
                     /locus_tag="PKL11_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ14070.1"
                     /translation="MHILLIHQAFAALDEAGGTRHIEMAQQLARQGHRVTIIASPVSY
                     LTGSAGSRPAWLTREEPLPGVTILRTYTLPSIHRSFVYRVFSFLSFMFSSFWVGLGVR
                     GVDLIWGTSPPIFQGWTAWFLSRLKRVPFLFEIRDLWPAFAIAVGVLKNKTLIHLSEW
                     LERFLYSHADRIIVNSPGFIQHVTSRGARQVDLIANGADPAMFTPEARGTTFRSEFNL
                     GDKFVVLYAGAHGLSNDLGIVLQAANHLRQRSDIVFAFVGDGKEKANLQQQAIDMDLP
                     NVIFAPPASKNQMAEVLAAADACIAILKPIEMYRTTYPNKVFDYMAAGRPVILAIDGV
                     IRQVVEEAQAGIFVPPGDSFALASAVGNLADNRQLAQSMGVSGRQAIETHYLRSLWTE
                     RFMDIILAMHQARH"
BASE COUNT          813 a          769 c          896 g          580 t
ORIGIN      
        1 ggctcggtgg ttttaagcga aaggaagatg gcgccatgga atgtaatcat cagcagggag
       61 accacgccgc ccagcagggc cagcggcggg accaggtcga agaatgtgcc aacaaagatt
      121 ttgctggcat ccacgggaac gcccaggata aaatctgcaa aggccacacc ccagagcagc
      181 gctgggagga gactgccgac aaaaatcgcc cagtcccaca ggctgcgcca tttggggttt
      241 tcgtctttac tgcgaaattc cagggcggcg ccgcgcagga tgaggccaat gaggatcagg
      301 aagagcggca gatagaagcc gctgaaaacg gttgcatacc agtggggaaa agccgcgaac
      361 atggccccgc cagctgtcaa aagccagacc tcgttgccat cccagtgggg tccgatggtg
      421 ttaatcacca cccggcggcg gatatcggtt ttggcgatga agggcagcaa gatgccaacg
      481 cccatgtcaa agccttcaag gacaaagtag ccaatgaaca gcacggcaat caagataaac
      541 cagacgatat tcagatccat gattgatact cctcttggtg aaccctagtg ccgggcagta
      601 tggtgagcgg gaacagtttt ggcgtatttc gccagcaaat agacatccgc ggccatcagc
      661 aaggcgtaga tcagcgtgaa gccaatcagg ctggtgagaa ccatccctga ggtcaggttg
      721 ggtgaaactg cctgagagag cgtcagttga tcgtaaacca cccagggctg ccggcccatt
      781 tcagtgagca gccagccaaa ggtgttggcc aggaagggca aggcaattgc aaacgggaag
      841 atgggtaaga gtttggcggg cacaggtttc ttgcgccaga tcataaagac cagatagagc
      901 gccagcacaa tcatcagggt gccgatgccg accatcaaac ggaaggtcca gaaggtcatg
      961 gcaaccggcg ggatgtaatt gccgggaccg tgtttggctt caaactcttt ctgaaggtcg
     1021 agcatacctt tgacctcgcc ggagaagcgg ttgtaagaga ggaagctcaa catgcccggg
     1081 atacgcagag caaacgtatt ctgctgggcg ttttcatcga tgccagcaac cagggagaag
     1141 gcggcgggat cttcggtgtg ccacagcgct tcggcagcgg ccattttcat cggttgggtg
     1201 cggacggtat tttggccttg agcgtgaccg ataaccgcaa cgcccagaga accaannnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgga aaccagccag atggcggcta
     1381 aatgcatttt cttggagagc ttttcccagc caaaaatcca gaggcccagg aaagtggatt
     1441 caatgaagaa ggccagaagc gcctcgatgg ccaggggagc gccgaaaata tcgccaacat
     1501 agcgtgagta ttcggaccag ttcatgccaa actgaaattc ctggacgata ccagtaacca
     1561 cacccatcgc aaagttgatc acaaagagtt tgccccaaaa ctgggtctgg cgtttgtggg
     1621 cttcctgtcc ggtgcggaca tagcgggtct ctaaaatggc gaccagcaaa cccaacccca
     1681 gcgtcagcgg tacaaaaaag aagtgataga tggtggtgat ggcaaactgc caacgcgata
     1741 gaatcagggt caactcatcc atagatgcgc tcctattatc caaacgattg aatcagggat
     1801 ggttaatttt acctgtaaac cgatgaattt gacaaataaa atcaggtcag ttttcccggc
     1861 gtataaggtt ttaatgccgg gcttggtgca ttgccaggat aatgtccatg aaccgttcgg
     1921 tccaaagcga gcggaggtaa tgcgtttcaa tggcctggcg acccgaaacg cccatgctct
     1981 gcgctaactg gcggttatct gccaggtttc ccacagcact cgccagcgca aatgagtcgc
     2041 cgggagggac aaaaatgccc gcctgagcct cctctaccac ctggcggatg acgccgtcaa
     2101 tggcaaggat gaccgggcgc ccggcagcca tatagtcaaa aaccttgtta ggataggtgg
     2161 tgcgatacat ctcaatcggt ttgaggatgg caatgcaggc gtcggccgcg gctaaaacct
     2221 cagccatttg attttttgaa gccggggggg cgaaaatcac attgggtaga tccatatcaa
     2281 tagcctgctg ttggaggtta gccttttctt tgccatcgcc cacaaaggca aaaacaatat
     2341 ctgagcgctg acggaggtga ttggccgcct gaaggacaat gcccagatcg tttgagagac
     2401 catgggcacc ggcatagagc accacgaatt tatcgccaag gttgaattcg ctgcgaaaag
     2461 ttgttccgcg cgcttccggg gtgaacatgg cgggatcggc cccattggca attaagtcca
     2521 cctgccgggc gccgcgagac gtgacgtgtt gaataaagcc gggcgaattg acaatgatcc
     2581 ggtcggcgtg cgaatagaga aaccgttcca gccactcaga cagatggatg agagttttgt
     2641 ttttcaaaac accaactgcg atggcgaacg cgggccagag atcacgaatt tcaaagagga
     2701 agggaactcg tttcaggcgg gagaggaacc aggcagtcca accctgaaaa attggcggcg
     2761 atgtgcccca gatcagatca acgccgcgaa cccccaggcc aacccagaag gatgagaaca
     2821 taaagctgag aaagctgaac acacgataga caaagctgcg gtgaatggaa ggcaaggtgt
     2881 aggtacgcag gatggtgacg ccaggcaagg gttcttcgcg tgtcagccag gccgggcggc
     2941 tgccggctga gccggtaaga tagctgaccg ggctggcgat gatggtgaca cgatgcccct
     3001 gccgggcaag ctgctgcgcc atttcgatat gccgggtgcc gccagcttcg tccagagccg
     3061 caaaggcctg atgaatcagc aagatgtgca tgggggctat tgtatcttat tttaagacat
     3121 ggcaccaatt caaaagccct aatggctaca cgaaatac
//
LOCUS       DAOPVS010000196         3134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_31850_length_3134_cov_38.171484, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000196 DAOPVS010000000
VERSION     DAOPVS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31850_length_3134_cov_38.171484"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKL11_10505"
     CDS             <1..680
                     /locus_tag="PKL11_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ14071.1"
                     /translation="IVTFFCAETLVPHPDQAARPKEVLGGYDRVFSDSRFVSFVGAMV
                     LNSVASTIMWVLLSVYAVKNYGLTESMYGLIPTTNALMVVLLQIPITQITRRYKVLPV
                     LMVGATLYALGVGSVAFGDGFSAFWLSMVIMSLGELVLMPTASTYTASLAPADMRGRY
                     MSVYGLTWSAGYAIGPSLGGFLNDRFGPQFIWLGGGAVGLMAAAIFALKARRAARQAA
                     LQPANHS"
     gene            complement(667..1155)
                     /locus_tag="PKL11_10510"
     CDS             complement(667..1155)
                     /locus_tag="PKL11_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ14072.1"
                     /translation="MKTVILMRHAKSSWKDPEITDHERPLNKRGEKDAPKMGKLLKEH
                     HLCPERIFSSSALRASQTADAVAAKCDYKEEIAYLDELYLAEPQAYLDLLINLPDEME
                     RVLIVGHNPGLEGLLQILSGAVNPLPTAAVAVLHLPIRTWKALTPNIEAELAHYWRPK
                     SD"
     gene            complement(1234..2163)
                     /locus_tag="PKL11_10515"
     CDS             complement(1234..2163)
                     /locus_tag="PKL11_10515"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HNZ14073.1"
                     /translation="MPVQPFAHTYSIVARDPLSGDFGVAVQSHWFSVGSVVAWAEPGI
                     GVIATQSMVEVSYGPLGLNLLRGGKTAEQTLQALLSTDEERELRQVAMIDAQGNAATH
                     TGKRCVKQAGHLVGNGFSVQANMMLNATVWTAMYAAYETALQDPAIDLPERLLRALEA
                     AQSCGGDIRGQQSAAIKIVSGTPRVKPWTGNLMDLRVEDHPAPVAELRRLVDIHRAYD
                     YMNQGDEYLGKGDEANAMLAYAAAARLAPQIVEILFWNAVSLANVGSLEEALPIFKKV
                     FASEPNWREMLARLVEPGLIKNDPETLEKILKV"
     gene            complement(2323..>3134)
                     /locus_tag="PKL11_10520"
     CDS             complement(2323..>3134)
                     /locus_tag="PKL11_10520"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNZ14074.1"
                     /translation="PTLRGMRRLGYTPEAIREFVARAGVAKTIVARSGVFVDLALLES
                     CVRDDLNKRAPRKMAVLNPLKVVIDNYPEGQVEEFDAVNNPEDETMGTRKVPFSKVIY
                     IEQDDFREEAPPKYFRLTPGREVRLRYAYFIKCESVVKDEAGNIVEIHCTYDPTTRGG
                     DAADGRKVKATLHWVSAAHAIPAEVRIYDRLFNNPDPETSGDFIADLNPNSLIIKNAL
                     LEPSLRDAQSGDRFQFERLGYFCADWDSKPEKLLFNRTVSLKDEWAKIEKQ"
BASE COUNT          621 a          952 c          800 g          761 t
ORIGIN      
        1 ccattgttac ctttttctgc gctgaaaccc tggtgccgca ccccgatcaa gccgcgcgtc
       61 ccaaagaagt tctcggcggc tacgaccgcg ttttcagcga tagccggttt gtctcttttg
      121 tcggcgccat ggtgttgaat tctgtcgcct ccaccattat gtgggtgctg ctgtctgtgt
      181 atgccgtcaa aaattacggg ctgaccgaaa gcatgtacgg cctgatccca accaccaatg
      241 ccctcatggt ggtcttgctg caaatcccca ttactcaaat cacccgccgg tacaaggttc
      301 tcccggttct tatggtcggg gcaacccttt acgctttagg cgtaggctcc gttgcctttg
      361 gtgacggctt ctcggcgttc tggttatcaa tggtcattat gtctttgggc gaactggttc
      421 tgatgcccac cgccagcacc tatacggcca gcctggcgcc cgccgatatg cgcggccgtt
      481 acatgagcgt ctatggcctg acctggagcg ccggttacgc cattggcccc agcctgggcg
      541 gctttttgaa tgaccggttt ggcccccagt ttatctggct cggcggcggc gcggtcggac
      601 tgatggcggc cgccattttt gcgctcaagg cgcgccgggc ggcccgtcag gcagctctac
      661 aaccggctaa tcactcttag gccgccaata gtgagccagt tcggcttcaa tattgggcgt
      721 cagcgctttc catgtgcgga tgggcaggtg caatacggcc actgccgccg ttggcagcgg
      781 gttgaccgct ccgcttagga tttgcagcag cccttccaac ccggggttat ggcccacaat
      841 cagcacccgc tccatctcat ccggcaggtt gatcagtaaa tccaggtagg cctgtggttc
      901 tgccagatac agctcatcca gataagcgat ttcctctttg tagtcgcatt ttgccgccac
      961 cgcgtctgcg gtctggctgg cccgcagtgc gctggagctg aagatccgtt ccgggcaaag
     1021 atgatgctcc ttgagtaatt tccccatctt gggcgcatct ttctcgccgc gtttattcaa
     1081 tggccgctca tgatcagtaa tttcgggatc tttccaggat gattttgcat ggcgcattaa
     1141 gattacggtt ttcatcggca gcttcctttt aggttgttaa gacaacatcc gaaaacaaac
     1201 agcgacagta tatcacagtc tggttctaaa atttcagact ttaagaattt tttccagcgt
     1261 ctcagggtca tttttaatca acccgggttc caccaggcgc gccagcatct ctctccagtt
     1321 tggctcgctg gcaaagacct ttttgaaaat gggcagcgcc tcttctaggc tgccaacatt
     1381 ggccagcgat accgcattcc agaaaagaat ttccacaatt tgtggcgcca gtcgggccgc
     1441 ggcggcatag gccagcatag cattggcctc atcccccttt cccaggtatt catcgccctg
     1501 attcatataa tcataagccc ggtggatgtc caccagccgg cgcagctctg ccactggcgc
     1561 agggtgatct tccaccctca agtccatcag gttccccgtc cagggtttga cccgcggcgt
     1621 acccgagaca attttgatgg ccgcactttg ctgcccgcga atgtctccac cgcagctttg
     1681 ggccgcttcc agcgcccgca gcagtctttc gggcagatca atggctggat cttgcagcgc
     1741 ggtttcgtaa gcagcataca tcgccgtcca gacggtcgcg ttcagcatca tgttggcctg
     1801 taccgaaaac ccgttcccaa ccaggtgccc ggcctgtttg acgcaccgct tgcccgtgtg
     1861 ggtggcagca ttcccctgtg catcaatcat tgccacctgg cgcagctcgc gctcctcatc
     1921 tgtgctcaac aaggcctgca aagtctgctc ggctgttttc ccgccgcgca gcaaatttaa
     1981 ccccaacggc ccataactga cctcaaccat cgattgcgtg gcaatcacgc cgatccccgg
     2041 ttctgcccat gcaaccaccg accccaccga aaaccagtga gattgcacag caacgccaaa
     2101 atccccgctg agtgggtctc tggcaacgat agaataggtg tgggcaaacg gttgaacggg
     2161 catctatctc tccttggggg gaagtccaaa cctgacaaaa tcacatattt caaaggtatg
     2221 ctgtcatttt agcattaaaa atgtcgtgcc tctcaaatta ataccaaaag cacagcctct
     2281 aattcaggct gtgcttttta attggctcaa aattgccggc ggctattgct tttcgatctt
     2341 agcccactca tctttcaggc tcacggttcg attgaagagc agcttctccg gctttgaatc
     2401 ccagtccgcg cagaaatatc ccaggcgttc aaactggaat cgatccccgg attgtgcatc
     2461 ccgcaggctg ggttccagca gggcgttttt gatgatcagt gagttgggat tcagatcagc
     2521 aatgaagtcg ccgctggttt ccgggtcggg gttgttaaac aaccggtcat aaatccgcac
     2581 ctctgccgga atggcatgtg ccgccgacac ccagtgcagc gttgccttga ccttgcgtcc
     2641 atcggctgca tcgccgccgc gcgttgtggg gtcataagtg cagtgaatct ccacaatatt
     2701 cccggcttca tcctttacca cgctctcgca cttgataaag tacgcatagc gcagccggac
     2761 ttcgcgcccg ggcgtcaagc ggaaatactt gggcggggcc tcttcgcgga agtcgtcctg
     2821 ctcaatataa atcacctttg agaaaggcac cttgcgtgtg cccatagtct cgtcttccgg
     2881 gttattaacc gcgtcaaatt cctcaacctg accttcggga tagttgtcaa tcacaacctt
     2941 taacgggttt aacaccgcca tcttgcgggg cgcccgcttg ttcaggtcat cgcgcacgca
     3001 ggattctagc agcgccaggt ccacaaatac gcccgaccgg gcaacaatcg tcttggccac
     3061 accggcccgc gccacaaact cccgaatggc ttccggcgta tagcccaggc ggcgcatgcc
     3121 gcgcagggtg ggca
//
LOCUS       DAOPVS010000197         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_31928_length_3127_cov_53.447591, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000197 DAOPVS010000000
VERSION     DAOPVS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31928_length_3127_cov_53.447591"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKL11_10525"
     CDS             <1..569
                     /locus_tag="PKL11_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ14075.1"
                     /translation="EPERVWNAYPHELSGGMRQRVLIALALLLEPQLLILDEPTTALD
                     ILTQRNIMDVLKELRTKLDFAMVFISHDLSLAAELADRVATMYAGKVVELADVYTTFK
                     NPRHPYTIGLIKAVPTLTTHKDHVSSIPGSPPDLINMPTGCKFHPRCPLADERCKRDE
                     PPLEPTTGTDHMVACWYWKTAHETLAAK"
     gene            580..1581
                     /locus_tag="PKL11_10530"
     CDS             580..1581
                     /locus_tag="PKL11_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ14076.1"
                     /translation="MPGPMIELKNISRSFHVKRNTYIQAVDNVSLSINEGEIVCLVGE
                     SGCGKTTTGRMIVGLLNPTKGQILYRGRDILHLDKAEQKEYRLGVQLVHQDPYASLNP
                     SFTIFDTMAAPLKRHGHTHNDAETRQRVIELLEMVDLTPAADFLDKYPHQLSGGQRQR
                     VSVARALTVNPSFIVADEAVSMVDVSIRISLLNTLLRLKEKMGLAVLFITHDLALAKY
                     FAWEGRIGVMYLGRMIEVAPARQLVEKPQHPYTQALLSAIPEADPDITRHKRHIQLRS
                     MDLPSLLNLPKGCTFHPRCPLWEAGLCDTVEPELTHFKDGSAVACHVISRQREGERQ"
     gene            1863..>3127
                     /locus_tag="PKL11_10535"
     CDS             1863..>3127
                     /locus_tag="PKL11_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ14077.1"
                     /translation="MRTHKFLILVVLLALVLGACTPVATEVPTEVVTEAPTEVVTEAP
                     TEVATEVPTAVPTEAGPLPEGVANILTINLTPGLKWSDGTELTAKDLVGTYNIYWLQK
                     ASVWTYLKDVVAVDDYTVEFWISSPSPRVLRLILRANQPAPYSQYSKWMDEAAKMRAD
                     GKAADSDEGKALLDDLYAFKPETLVVYGPYVIDTEGVTEAQLELLKNPTGYNADKVAF
                     EKVLLYYGETAASVPLVLSQEIDYSTHGYTPSDVEQFQTMENIKILTGPTGTGPGLWF
                     NQNVYPLNKKEVRQAFAYIINREENATVAMGPAGRAIEYEAGFTDLQVDAWLTDETRN
                     ALNKYETDWTKAEELLTGIGFTKNADGKWLDDTGTLIDIELWVPADFADWLGSAENAA
                     QQLNAFGITTTVRGYPSAERATTQKEGKY"
BASE COUNT          722 a          839 c          886 g          680 t
ORIGIN      
        1 tggagcccga gcgggtttgg aatgcttacc cgcatgaact ctcgggaggg atgcggcagc
       61 gggtattgat tgcgctggca ctgctgcttg agcctcaact gctgattctg gacgagccca
      121 cgacagcgct ggatattttg acgcaacgaa atatcatgga tgtgctgaag gaactgcgca
      181 ccaagctgga ttttgcgatg gtctttattt cgcacgatct ttcgctggcg gcagagctgg
      241 ctgaccgggt ggcgacaatg tatgccggca aggtagtgga gctcgccgat gtgtacacca
      301 cgttcaagaa cccccggcat ccgtacacca tcgggttaat caaggctgtg ccgacattga
      361 cgactcataa agatcacgtt tcgtcgattc cgggttcgcc gccggacttg atcaatatgc
      421 cgacagggtg caaattccat ccgcgctgcc cgctggcaga tgaacgctgc aagcgggacg
      481 aaccgccgct ggagccgact acaggaaccg atcatatggt tgcctgctgg tactggaaaa
      541 ccgcccacga aaccctggct gcaaaataag aggatgccca tgcctggacc aatgattgaa
      601 ctcaaaaata tctcgcgcag ctttcatgtt aaacgcaata cctatattca ggctgtggat
      661 aatgtctcgc tcagcattaa tgagggtgag attgtctgcc tggtcggcga gtcgggctgc
      721 ggcaaaacaa ccaccgggcg gatgattgtg gggctgctaa atccgacgaa gggtcagatt
      781 ctgtatcgcg ggcgagacat tttgcacctc gataaggcgg agcagaaaga ataccggctg
      841 ggtgtgcagt tggttcatca ggacccctat gcatcgctga atccttcttt tacgatattt
      901 gatacgatgg cggcaccgct gaagcggcac gggcataccc acaatgacgc cgagacccgc
      961 caacgggtga ttgaactgct ggaaatggtt gatttgaccc ctgcggcgga ttttctggat
     1021 aaatatcccc accagttgag cggcggacag cggcagcgag tctcagttgc ccgggcgctg
     1081 acggtcaatc cttcttttat tgtggcggat gaggcggtct cgatggtgga tgtctccatc
     1141 cggatcagcc tgctaaacac actgctgcgg ctcaaagaaa agatggggct ggcggtgctg
     1201 ttcatcacac acgatctggc gctggccaag tattttgcct gggaagggcg cattggggtg
     1261 atgtacctgg ggcggatgat tgaggttgcc ccggcaagac aactggtgga aaaaccccag
     1321 cacccctaca cgcaggcttt gctgtctgcc attccggaag ctgacccgga tattacccgg
     1381 cataaacggc acattcaact gcgcagcatg gatttgccca gcctgctgaa tttgcccaaa
     1441 ggctgtacgt tccacccgcg ctgccccctc tgggaagcgg ggttgtgcga cacagtggag
     1501 ccagaattaa cgcacttcaa agatggttcg gcggtggcat gtcacgtcat cagccgccag
     1561 cgtgaaggag agcgccagtg agccctgagc taggtcggac ctggttccga gtggcttttt
     1621 tcattaccct gatgtcggca ctgttattgc tggtggtcaa gcctgggact gctgaatttg
     1681 tcataacagt gtttaccctg gcaattggat tgatctttat ggctgtgatc atcattattg
     1741 cccggaaatc aaagtaggaa aatgcaatca aaggaggtga tttctgcaag ttcacgaaac
     1801 aagttggaaa cgattgaaat ttcgacccat ttcaaatttt ttcactctca gaggagaagc
     1861 aaatgcgtac ccataagttc ttaattcttg tagtgctcct ggccctggtg ctgggcgcct
     1921 gcaccccggt tgctactgag gtcccgaccg aagttgtgac cgaagctccg accgaggtcg
     1981 tcaccgaggc cccgactgag gtggctacag aagttcctac ggctgttccg accgaagctg
     2041 gcccgctgcc cgaaggcgtt gccaatatcc tgaccatcaa cctgacccct gggctcaagt
     2101 ggagcgatgg caccgaattg accgccaagg atctggttgg cacctacaac atctactggc
     2161 tgcagaaagc ttccgtatgg acttacctga aggatgttgt ggcggttgat gattacactg
     2221 ttgaattctg gatcagcagc ccctcaccgc gtgtgctgcg cctgatcttg cgcgccaatc
     2281 agccggcccc ctactcgcag tacagcaagt ggatggacga agctgccaaa atgcgcgccg
     2341 atggcaaagc tgccgacagc gatgaaggta aagccctgct ggatgatctg tatgccttca
     2401 aacccgaaac cctggtggtc tatgggcctt acgtcattga caccgaaggc gttactgaag
     2461 ctcagcttga gctgctgaag aaccccaccg gttacaatgc cgacaaggtt gcctttgaaa
     2521 aagttctgct atactacggt gaaaccgctg cctctgtgcc gctggttctc tcgcaggaaa
     2581 ttgactacag cacgcatggt tacaccccgt ctgatgttga acaattccag accatggaaa
     2641 acatcaagat cttgaccggc ccaaccggca ccggcccggg tctgtggttc aaccagaacg
     2701 tgtatccgct gaacaagaag gaagtccgcc aggcgtttgc ctatatcatc aaccgcgaag
     2761 agaacgccac ggttgcgatg ggcccggcgg gccgcgcgat tgaatacgaa gctggtttca
     2821 ccgacctgca ggtggatgcc tggctcaccg atgagacccg caacgctctg aacaaatacg
     2881 aaaccgactg gaccaaagcc gaagaactgc tgacaggcat tggcttcacc aagaatgcag
     2941 atggcaagtg gctggatgac accggcacgc tcatcgacat tgaactgtgg gtgcccgctg
     3001 actttgcgga ctggctgggt tcggctgaaa atgccgcgca gcagttgaat gcctttggca
     3061 tcaccaccac ggtgcgcggt tatccgtctg cggaacgcgc caccacccag aaggaaggca
     3121 agtacga
//
LOCUS       DAOPVS010000198         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_32882_length_3062_cov_47.101097, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000198 DAOPVS010000000
VERSION     DAOPVS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32882_length_3062_cov_47.101097"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PKL11_10540"
     CDS             <1..1409
                     /locus_tag="PKL11_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ14078.1"
                     /translation="GRFHGPVSVRSALANSFNIPAVKALEYIGIYDNPATEWPDGLVP
                     FAQRLGITTLTRNDYGLALTLGGGDVSLLEMTGAYAVFANGGQQVPPVAITRIVDHEG
                     NVVYEYEPPEPVQALRSEHAYLISSILSDNAARTPMFGANSVLNLPFAAAAKTGTTND
                     YRDNWTIGYTPDLVTGVWVGNADYTPMQNTTGLTGAAPIWAQFMQFAVPQLTGGYPTP
                     FTRPPGVVDLVVCSVSGAQPSQWCPEQRTEIFAADQPPAPADQDLWQKVLVDTWTGLR
                     ASPICAEYTSEEFAINVTDKSAIEWIRNTDEGRAWADSLGFEEPFFFTPERECTAEDE
                     RPLLFFSNLTEGQTITESPLDVWVVVSAVKDFKEFRLDYGLGSDPADWETLVEGLTAP
                     LNQPGLIGTWDISSLPAGPITLRLHLDSDRDTYAEKVIHINVQVPTLTPTPTPTPTQT
                     PTPTPTPTVTPTKPPDEP"
     gene            complement(1540..2865)
                     /locus_tag="PKL11_10545"
     CDS             complement(1540..2865)
                     /locus_tag="PKL11_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14079.1"
                     /translation="MKKPSPLTRLSTRGWLLIVMLGAAVERLLLYFTYRPMLYKDTDA
                     YRRLAGQVLAGWADYDGSRMPGYPVFLAGLGTDETVYAVQLALGLLITLLFFYIGWRL
                     TGKGWAGALFAAAYTLNPQQVLVEADLLTETLTVFFLAVSLAALTALLCRPGPRPLWQ
                     TLALAVLAGAAAGLAALTRTLFVFLPVLAGFFLVVGWPQARRGVRWAAALTTALVGLA
                     LIGVYVNFIHQRFDIWGLSTMNGYHMMNHAGQFFEYVPDEYAGLRETYIRYRDAQQAE
                     TGSGANAIWDAIPEMQQVSGLGFTDLSRQLGQICTRLILEHPGLYLRSVSRGWVGFWK
                     APVHWRTLTGGLAGAQAAAITALRGVLMLANLIFLLGTLGLIFPRLRHRLQLNTPLYL
                     MGASVWAASIMQAMLEFGDNPRYSVPVQTLVLLLVGWGVKKVAGLRFRA"
BASE COUNT          629 a         1015 c          894 g          524 t
ORIGIN      
        1 atggccgctt ccacggcccg gtttcggtgc gctcggcgct ggccaattca tttaacatcc
       61 ctgcggtcaa ggcgctggaa tacattggca tttatgacaa ccccgccact gagtggcccg
      121 atggattggt gccgtttgcc cagcggctgg gcatcaccac gctcacccgc aatgattatg
      181 gcctggcgct gacgctgggc ggcggtgatg tgagcctgct ggagatgacc ggcgcctatg
      241 ccgtctttgc caacggcggg cagcaggtgc cgccggtagc catcactcgg attgtggatc
      301 acgaaggcaa cgtggtttac gaatatgagc cgcccgagcc cgtgcaggcg ctgcgctccg
      361 aacacgccta cctgatcagt tcgatcctct cggacaatgc cgcccgcacg ccgatgttcg
      421 gcgccaattc ggtgctcaat ctgccgtttg ccgcggccgc caagaccggc accaccaacg
      481 actaccgcga taactggacc attggctaca cccccgacct ggtgacgggc gtgtgggtgg
      541 gcaatgccga ttacaccccc atgcagaaca ccaccggcct caccggtgcg gcgcccatct
      601 gggcgcagtt tatgcagttt gccgtgccgc agctcaccgg cggctacccc acccccttca
      661 cccgcccgcc cggcgtggtg gatctggtgg tctgctcggt ctccggggcg cagccttcgc
      721 agtggtgccc cgagcagcgc accgagatct ttgctgccga ccagccgccc gcccccgccg
      781 accaggacct gtggcaaaaa gtgctggtgg atacctggac ggggctgcgc gcctcgccga
      841 tttgcgccga gtacacctcg gaggaatttg ccatcaacgt gaccgataaa tcggccattg
      901 agtggattcg caacaccgac gaaggccgcg cctgggctga ttcactgggc tttgaagaac
      961 ccttcttctt tacccccgag cgtgaatgta ctgctgaaga cgagcgcccg ctgctgttct
     1021 tctccaacct caccgagggt cagacgatca ccgaaagccc gctggatgtc tgggtggtgg
     1081 tcagcgcggt caaggatttc aaagaattcc ggctggacta tggcctgggc agcgaccccg
     1141 ccgactggga aaccctggtg gaaggcctga ccgcgcccct gaaccagccc ggtttgatcg
     1201 gcacctggga tatcagcagc ctgcccgccg ggccaatcac cctgcggctg cacctggaca
     1261 gcgaccggga tacctacgcc gagaaagtca tccacatcaa cgtgcaggtg cctacgctca
     1321 cccccacgcc tacccctacc cccacgcaga cgcccactcc cactcccacg ccaaccgtca
     1381 cccccaccaa acccccggac gaaccgtagg atttaggatt taggatttta gatttgaaat
     1441 ttgagattga ggataaagca gcggttgagc cagtttttac ccggcttaac cgctgcccta
     1501 aaacctaaat cccaaaacca aaaacctgaa tccccaaacc taagccctga atcgcaatcc
     1561 cgcaaccttc ttcacccccc agccaaccag cagcagcacc agggtttgca ccggcaccga
     1621 gtagcgcggg ttgtcgccaa attcgagcat ggcctgcata atggaggccg cccagacgct
     1681 ggcgcccatc aggtagagcg gggtgttcaa ctgcaggcga tgccgcagcc gggggaagat
     1741 cagcccgagg gtgccgagca gaaagatcag gttcgccagc atcagcacgc cgcgcagcgc
     1801 ggtgatggcc gcggcttgcg cccccgccag cccgcccgtc agcgtgcgcc agtggacggg
     1861 agccttccag aagcctaccc agccccggga taccgagcgc aggtacagcc ccggatgctc
     1921 cagaatcaac cgggtgcaga tctgccccaa ctggcgggag aggtccgtga agcccagccc
     1981 ggagacctgc tgcatctcgg ggatggcatc ccagatggcg ttggcgccgc tgccggtttc
     2041 ggcctgctgg gcgtcgcggt agcggatgta ggtctcgcgc aggccggcat attcatcggg
     2101 cacatattca aagaactgcc cggcatggtt catcatgtgg tagccgttca tcgttgaaag
     2161 cccccagatg tcgaaccgct ggtgaatgaa attgacatat accccgatca gcgccagtcc
     2221 caccagggcg gtggtcagtg cggcagccca gcgcaccccg cggcgggcct gcggccagcc
     2281 caccaccagg aagaagcccg ccagcaccgg cagaaagaca aacagcgtgc gcgtcagcgc
     2341 ggccaggccg gccgcggccc ccgccagcac cgccagcgcc agcgtctgcc agagcgggcg
     2401 cggccccgga cggcagagca gtgccgtcag tgcggccagg ctgacagcca gaaagaagac
     2461 cgtcagcgtt tcggtcagca ggtcggcttc taccagcacc tgctgcgggt tgagcgtgta
     2521 ggccgcagcg aagagcgccc cggcccagcc tttgcccgtc agccgccagc cgatgtagaa
     2581 gaacagcagt gtaatcagca ggcccagcgc caactgcacc gcgtaaacgg tctcgtcagt
     2641 gcccagcccg gccagaaaaa ccgggtaccc cggcatgcgg gagccatcgt agtcggccca
     2701 gcccgccagc acctgcccgg ccagccgccg gtaggcatcg gtatctttgt agagcatcgg
     2761 acggtaggtg aagtacagca gcaggcgttc aaccgccgcg ccgagcatca caatcagcag
     2821 ccagccgcgg gttgaaaggc gggtgagggg tgagggtttt ttcatatggg caaatcttat
     2881 cacggaatga cggccttggg ctgccgcaaa aattttacaa aaaaaagcaa aagtcacctc
     2941 ttgtattttt agcaaatcgt tatatacttc tcggctgatt gtttttttta aaaacaatcc
     3001 atcttgcatc ggagtaaaaa ctgtaccatg gatcttgatc tcgagctcga acaagccatc
     3061 gc
//
LOCUS       DAOPVS010000199         3061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_32906_length_3061_cov_51.276780, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000199 DAOPVS010000000
VERSION     DAOPVS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32906_length_3061_cov_51.276780"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKL11_10550"
     CDS             <1..332
                     /locus_tag="PKL11_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNZ14080.1"
                     /translation="STGLYGKTLLPQNGAPLRLVVPWKYGFKSIKSIVKIELVEEMPT
                     SLWMDAAPSEYGFYANVNPDVAHPRWSQATERRIGDLGRRDTLLFNGYEEAVASLYSG
                     MDLRQNY"
     gene            332..955
                     /locus_tag="PKL11_10555"
     CDS             332..955
                     /locus_tag="PKL11_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNZ14081.1"
                     /translation="MRLKFTRFQGLVHLAAFAPLVVLIIQAIADRLTANPIQAITQRT
                     GQAAVIGLLLTLACTPINTITGWRAVLTVRRALGLWAGFYALLHFLTFAMLDYGLDLR
                     QIGRAILEKPFILVGLITLLVLIPLAITSTRAAMRKMGARWKKLHRLSYLAAVLAVIH
                     YLLAAKADFAAQPLIYGSILGLLMMLRIKPVRQWFIRRRASAATAED"
     gene            complement(952..2220)
                     /locus_tag="PKL11_10560"
     CDS             complement(952..2220)
                     /locus_tag="PKL11_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ14082.1"
                     /translation="MKTTEWIEEIAASREQAATHPHPQAQPLAAFAGIHPAILQLASV
                     YSSPLLAGPPLCEELVSLVRHMFTDEQAELTRHITMPLGASAIEIAGMAERPVAEVEP
                     ILEYLANVRGVIFSLGTASWKRYFLLPLVPGVFEMVLMHTSEADLTDWHREFAARFRK
                     LFETGYFGDYGRRPVELGYYLPVREAIPAQSIALPSDHLDLVLDRYKVFGVGLCQCRL
                     SKRLTGGDACSLPMETCITFGGIAERLIQNGRLRKVTRRDVFTIKAEAEDAGLVTLML
                     AANLKPDVAGISSGMACSCCSDCCYALKVIRDFNAPGIVAAPHFRPNIKALACTACGR
                     CVSVCPVSALTIDPVARKLVINPQRCIGCGLCSRTCARHAIEMRPVPNYHHPPQTALG
                     LAAHALPNFARNVWRIWRRRTRRDAPASER"
     gene            complement(2313..>3061)
                     /locus_tag="PKL11_10565"
     CDS             complement(2313..>3061)
                     /locus_tag="PKL11_10565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ14083.1"
                     /translation="ANSKLYRLDEFRAGQNKLDALVTGELGPVQGQSLLHLQCHFGMD
                     TLSWARLGASVTGVDFSPEAINLATRLSQELNIPARFICSELYDLPAHLDEQFDIVYT
                     SYGVLTWLPDIRGWAQLVARYLRPGGVFYIAEFHPFAMVFSDAVSEPRWQVDYDYFDS
                     SPMAFPVQGSYADHTAEVKQTTSYEWQHTLGDIVTALIDAGLTIEFLHEHDFTVYEMY
                     PFVTRAEDGYWRLPPGLPRLPLMFSLRAHR"
BASE COUNT          664 a          802 c         1005 g          590 t
ORIGIN      
        1 tatccaccgg gctgtatggc aaaacgctgc tgccacaaaa cggcgcgcca ctgcgactgg
       61 tggtgccgtg gaagtacggg tttaagagca ttaagtcaat cgtcaagatt gaactggtag
      121 aggagatgcc gacctcgctg tggatggatg ctgcgcccag cgagtacggg ttttatgcca
      181 atgtgaatcc cgatgtggcg cacccgcgct ggtctcaggc gacggagcgg cggattggcg
      241 atttggggcg gcgggatacg ctgctgttca atggctacga agaggcggtt gcttcgctct
      301 acagcggcat ggatttgagg cagaattact gatgcggctg aaatttaccc gctttcaagg
      361 actggttcat ctggcagcgt ttgcgccgct ggtcgttttg attatccagg cgatagctga
      421 ccggctgacg gccaacccga ttcaggccat cacccagcgg accggacaag cggctgtgat
      481 tggtctgctg ttgacgctgg cctgtacgcc gattaacacc atcacggggt ggcgggctgt
      541 gctgaccgtc cggcgggcgc tgggattgtg ggccgggttc tatgcactgc tgcattttct
      601 gaccttcgcc atgctggatt atgggctgga tttgcggcaa attgggcgag cgatacttga
      661 aaagccgttc atcctggtgg ggctgatcac actgctggtg ttgatacccc tggcgatcac
      721 atccacccgg gcagcgatgc ggaaaatggg agcgcgctgg aagaagctgc accggctgag
      781 ctatctggct gcggtgctgg cggtgattca ttacctgctg gcagctaaag ctgattttgc
      841 cgcccagccg ctgatttacg gcagcattct gggcctatta atgatgctgc ggatcaagcc
      901 agtccggcag tggtttattc ggcggcgcgc cagcgcggca acggctgaag attaacgctc
      961 agaggccggt gcatctctgc gggtgcggcg gcgccagatg cgccagacat tgcgcgcaaa
     1021 gttaggcaac gcatgcgccg ccagccccag ggcagtttga ggcggatgat gataattggg
     1081 caccgggcgc atctcgatgg catggcgggc acaggttcgg gaacacagac cacaaccaat
     1141 gcagcgctgc gggttgatga ccagcttgcg ggccacgggg tcaatcgtca gggcgctgac
     1201 cgggcaaacc gatacacagc gtccgcaggc agtgcaggcc aacgctttga tattagggcg
     1261 gaagtgcggc gcggcgacga tgccgggggc gttaaaatcg cgaatgactt tgagggcata
     1321 acaacaatct gaacaacagg aacaggccat gccgctgctg attccggcca catcgggctt
     1381 gaggttggca gccagcatga gggtgaccag gccggcgtct tcggcttcgg ccttaattgt
     1441 gaagacatcc cggcgggtga ctttgcgcag gcggccattc tggatcaggc gctcggcgat
     1501 accgccaaag gtgatgcagg tttccatggg caggctgcag gcgtcgccgc cggtcaggcg
     1561 tttggagagg cggcactggc acagacccac gccaaagacc ttgtagcggt ccagcaccag
     1621 gtcgaggtga tcggagggca gggcgatgga ctgcgccggg atggcctcgc gcacgggcag
     1681 gtaatagccc aactccaccg gacggcggcc atagtcgccg aagtagccgg tttcaaagag
     1741 tttgcggaag cgggcagcaa attcccgatg ccagtcggtc aggtcagcct cggaggtgtg
     1801 catgagaacc atctcaaaga cgccgggaac caggggcagg agaaagtaac gcttccagga
     1861 ggccgtgcca aggctgaaga tcacccctcg gacgtttgcc aggtattcca gaatgggctc
     1921 aacttcggct acggggcgct ctgccatgcc cgcaatttca attgcagagg ctccgagcgg
     1981 catggtgata tgccgggtaa gttcagcctg ctcatcggtg aacatgtgcc gaaccaggga
     2041 gaccagctct tcgcaaagcg gcggacccgc cagaagcgga ctggaataaa cggaagccaa
     2101 ttgaagaatt gcgggatgaa tcccggcaaa tgccgccagg ggctgtgcct ggggatgggg
     2161 atgcgtggcg gcctgctcgc gcgaggccgc tatttcttca atccattcgg tggttttcat
     2221 ttcaatccaa acttaatcca aacagcctga ttgggcttat tttcattata aaagtatttg
     2281 ttaggcgagg caagccagag ttcagaatgg tcttagcggt gggcgcgcaa tgagaacatg
     2341 agcggcaggc gcggcagccc ggggggcaag cgccagtagc catcctcggc gcgggtcacg
     2401 aaggggtaca tctcgtacac ggtgaagtcg tgttcgtgca agaattcaat cgtcaggcca
     2461 gcatcaatca gcgcggtgac gatatcgccc agggtgtgct gccactcata agaggtggtt
     2521 tgcttgacct cggcggtatg atcggcataa gagccctgga cgggaaaagc catcggggaa
     2581 gagtcaaagt aatcataatc cacctgccag cggggctcgg agactgcatc tgagaaaacc
     2641 atagcaaagg ggtgaaactc tgcaatgtag aagacgccgc cggggcgcag gtaacgcgcc
     2701 acaagctgcg cccagccgcg aatatcgggc agccaggtca gcacgccgta ggaggtgtag
     2761 acgatgtcaa actgctcatc caggtgagca gggagatcgt ataactcgct gcaaataaag
     2821 cgggcgggaa tgttcagttc ctggctgagg cgggtcgcca ggttgattgc ctcgggggag
     2881 aaatctacac cggtgacaga cgcgcccaga cgagcccaac tgagggtatc catgccaaaa
     2941 tggcactgga ggtgaagcag ggattgaccc tgtacgggac ccagttcgcc ggtgaccagg
     3001 gcatcgagct tgttttgacc ggccctaaat tcatccaacc gatacagttt tgagttggca
     3061 t
//
LOCUS       DAOPVS010000200         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_33160_length_3045_cov_42.461538, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000200 DAOPVS010000000
VERSION     DAOPVS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33160_length_3045_cov_42.461538"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /gene="nuoF"
                     /locus_tag="PKL11_10570"
     CDS             <1..1418
                     /gene="nuoF"
                     /locus_tag="PKL11_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNZ14084.1"
                     /translation="LVEEHLYKGRVAAPLQASVRELSGKIAWLQARKGTLPAEQRIVL
                     ERAGVIDPTSIEDYIVHDGYAALGKALTEMTPQQVIETLSKAGLRGRGGAGFPTGTKW
                     GFVAKTPGKVKYVVCNADESEPGTFKDRLILEGDPFSIIEAMTLAGYAVGASVGYIYV
                     RGEYGLAQERLHNAIAQAEEMGFLGKNLFGTDFSFEIHVHGGAGAYICGEETALIESI
                     EGKRGEPRSRPPYPTTHGLWGKPTLVNNVETLANVAPIIRNGAEWFRSFGTPSSPGTK
                     VYTILGNTNVTGLIEVPMGVTLREVLAIYGKGMKNGANFKLAQTGGSSGSVIPASLQD
                     TPMDFDAFNRAGVALGSGALLICDEETCVVDLARVLLKFFEVESCGKCTPCRVGNERA
                     YQILTNIAEGVGTLQDLDDLLMLSDNLYQLSNCGLGQTAGTPIRDVLTYFRAEVEAHI
                     RLKVCPAGVCSMSGHKVYQIN"
     gene            1455..>3045
                     /locus_tag="PKL11_10575"
     CDS             1455..>3045
                     /locus_tag="PKL11_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNZ14085.1"
                     /translation="MINLTIDGKAISVTEGTTVLQAAREAGIEIPTLCDHPELTPYGG
                     CRLCLVEVEGARTLQPSCTLPVNNNMIVRTNTDRIKAARKFVLTLIFSERNHFCPYCQ
                     VSGGDCELQNAAYDEGMTHWPLQPNWQPYPVDASHPYIILENNRCILCRRCVRACGEL
                     VGNFTLGFEGRGARNLLVADLGVPLGESTCISCGVCVQVCPTGALIDRWSAYRGRETQ
                     VDRTDTICTGCSIGCGMTMLTRDNNLVRIEGDWEAPANDGLLCEIGRFKPMAETRHRI
                     NTPLVRKDGTLKAATWDEAMKVIADRMKPAGKKVAALVSSRMSAEALYGFKQIFAEQL
                     HSEMVTSIEEGAYTATAAKLADKMGSAYEGQMSDLVSADGILVVGTDLVKEHQVVGFL
                     VKRIAPAGVPVVLVDEGDNKLDTAANFVFKAAKGHAELALRALTAAVIRLGLNKKSVK
                     IADGLVEEFAAKAGLKAEDILEAGRALAEAEHPVFVYGKLLTAPEAGEALEALLNLAA
                     VTGAKVISTKGEANSLAASQYH"
BASE COUNT          649 a          816 c          952 g          628 t
ORIGIN      
        1 ttctggttga agagcacctc tataaaggcc gggtggcggc gccgctgcaa gcttcagtac
       61 gtgagctgag cggcaagatt gcctggctgc aagcccgcaa gggaaccctg cccgcagaac
      121 aacggattgt gctggaacga gccggggtga ttgacccaac cagtattgaa gattatattg
      181 ttcacgatgg gtatgcggcg ctgggcaagg cactgaccga aatgacgccc cagcaggtga
      241 ttgagaccct ctccaaggct ggtctgcgcg gccgcggcgg tgcgggcttc cccactggaa
      301 cgaaatgggg ctttgtagct aagacccctg gcaaagtgaa gtatgtggtc tgcaatgctg
      361 acgaaagcga gccagggact ttcaaagatc gcttgattct ggaaggcgac cccttcagta
      421 tcatcgaagc catgacgctg gcgggttatg ccgttggcgc gagcgtaggt tacatctacg
      481 tgcgcggcga atacggcctg gcgcaggagc gtttgcacaa cgcgattgca caggctgaag
      541 agatgggctt cctgggtaag aacctgtttg gtacagattt cagctttgaa atccatgtac
      601 acggcggtgc aggggcctat atctgcggcg aagagactgc cctgattgaa tccatcgagg
      661 ggaagcgtgg cgagccgcgc tcccgcccac cctacccgac cacgcacggc ctgtggggca
      721 aacccacgct ggtcaataat gtggaaacgc tggctaacgt ggcgcctatc attcgcaatg
      781 gagcggaatg gttccgcagt tttggaacgc cctccagccc gggcaccaag gtgtacacca
      841 tcctgggcaa tacgaatgtg accgggttga ttgaagtacc catgggggtt acgctgcgag
      901 aagtactggc gatctacggt aagggaatga aaaatggcgc caacttcaag ctggcgcaaa
      961 ccggcggctc aagcggatcc gtcattccgg cgagtctgca ggatacaccg atggactttg
     1021 acgcttttaa ccgggcgggg gtggcactgg gctctggcgc gctgttgatc tgcgacgaag
     1081 agacctgtgt tgtggaccta gcccgggtac tgctcaagtt ctttgaggtg gaaagctgtg
     1141 gtaagtgcac cccctgccgg gtcggcaatg agcgcgctta tcagattttg accaacattg
     1201 ctgaaggcgt tgggacgctg caagacctgg acgacctgct gatgttgagc gataacctgt
     1261 accagctttc taactgcggc ctgggtcaga ctgccggaac ccctatccga gacgtgctga
     1321 cttacttccg cgctgaagtg gaagctcata ttcgtctgaa agtctgcccg gcgggcgttt
     1381 gctcgatgag cggacataaa gtttaccaaa tcaattaacg caatcatagc gaacctgctg
     1441 aggagatgtt gagtatgatc aacctgacga ttgatggaaa agcaatttct gtgaccgaag
     1501 gcaccacggt gctgcaggca gcccgcgagg ctggcattga aatcccgacc ctgtgcgatc
     1561 accctgagct gaccccttac ggtggctgcc gtctgtgcct ggtggaagta gaaggggcgc
     1621 gcacgctgca gccctcctgc acactgccag tcaacaacaa tatgattgtg cggactaata
     1681 ctgatcggat taaggctgcg cgcaagtttg tgctgaccct gatcttttca gagcgcaatc
     1741 acttctgccc ctactgccag gtgagcggcg gcgactgcga actgcagaac gctgcctatg
     1801 acgaaggtat gacccactgg cccctgcagc caaactggca gccctacccg gtggatgctt
     1861 cacacccgta catcatcctg gaaaacaacc gctgcatcct ctgccgccgc tgcgtgcggg
     1921 cctgcggcga actggtgggc aactttacgc tcggttttga aggccgcggg gcgcgcaacc
     1981 tgctggttgc cgacctgggt gtaccgctgg gcgaaagcac ctgtatctcc tgcggcgttt
     2041 gtgtgcaggt ttgccccacc ggcgcgctga ttgaccggtg gagcgcttac cgtggtcgcg
     2101 agacccaggt ggaccgaact gatacgatct gcacgggctg ctcgattggc tgcggcatga
     2161 ccatgctgac ccgtgataat aacctggttc ggattgaggg tgactgggaa gccccagcga
     2221 acgatggttt gctgtgcgaa attggccgct ttaagccgat ggccgaaacc cggcaccgca
     2281 tcaatactcc gctggtgcgc aaagatggca cgctcaaggc cgccacctgg gatgaggcaa
     2341 tgaaagtgat tgccgaccga atgaagcccg cgggcaagaa agttgcggca ttggtttcca
     2401 gccggatgtc ggctgaggcg ctgtacggct tcaagcagat ttttgctgag caactccaca
     2461 gcgagatggt gacctcaatt gaagaaggtg cctataccgc cactgccgcc aaactggctg
     2521 ataagatggg ttctgcttat gaagggcaga tgtctgacct ggtcagcgcg gacggcattc
     2581 tggttgtggg aaccgatctg gtcaaggagc atcaggttgt gggcttcctc gtcaaacgga
     2641 ttgccccggc gggtgtgccg gttgtgttgg ttgacgaagg cgataacaag ctcgatacgg
     2701 cggctaattt tgtgttcaaa gccgctaagg gccatgctga actggcgttg cgggcactga
     2761 ctgccgcagt gatccgcctg ggcctgaaca agaaatctgt caagattgca gatggcctgg
     2821 tggaagaatt tgccgccaag gctggtttga aagccgaaga tatcctggaa gctggccgcg
     2881 ccctggctga ggctgaacac cctgtgtttg tttatggcaa gctgctgacc gctcccgagg
     2941 cgggtgaggc gcttgaggcg ctgctgaacc tggcggccgt gaccggtgcc aaagtgatca
     3001 gcaccaaggg cgaggcgaac agcctggctg cttcgcagta ccacc
//
LOCUS       DAOPVS010000201         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_33417_length_3030_cov_63.559328, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000201 DAOPVS010000000
VERSION     DAOPVS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33417_length_3030_cov_63.559328"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PKL11_10580"
     CDS             complement(<1..1241)
                     /locus_tag="PKL11_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ14086.1"
                     /translation="MPFELPVILFTDIEHSTQIWQKYPVEADAAFRTHDQIVRSAIEN
                     HGGRVIKHTGDGFFAIFESGDPLTCAIKIQQSLASQNWGPIGELRVRVSLHAGAVISR
                     TDDFFGLEISRTERLLSAGWGGQILLSPEAAQQAPLPPGAELIDLGMHLLKDLTQPQP
                     ILQLNHPDLPYQKFPALRSLSAHPNNLPPQTTPFVGRAADLAAIGAQLQTPACRLLTL
                     VGSGGTGKTRMALQVAADSIEQFAHGVYFIPLAPLATADLIAPAIGQALSIPFHNRAT
                     LDEQVIQYLGQKQLLLVMDNFEHLLDGAVLVDQLLQRSTRLKILVTSRERLNLLGEQI
                     YPLQGMRYPQQPNDAEFEEYSAVKLFLQSAQRADPAFELNADNRAALIRVCELVEGLP
                     LGIELACGWVRVLSLTEIAAE"
     gene            1346..>3030
                     /locus_tag="PKL11_10585"
     CDS             1346..>3030
                     /locus_tag="PKL11_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14087.1"
                     /translation="MRRRVILGVLLLSMILMLGLMGNGYAIVRAEDGEETPEPYVRKT
                     TLTLSFLEHEWWLVRWKDNEIGCRVFIEHEGLPSSDEILTACGTSLHNQWLATKPCAQ
                     AESGEDVSKCSGLYLHYVEAVPGERTVEVELPLPTVWISLTGCEDMDAEGRCANIPTL
                     LLSGEEPLPNEQIISIAGTINGEPFSCPGSTCEIPLQPTGSQGVEALFWAESSFGDSS
                     PEYTALVRVQPWGDFMSPDGGGSDPQRWYADVLSTQWRGGPLASCSDIWQVFPEMGGP
                     PPWLDTPAQPEDLYSSYSFYFLAGMLISNGEVDASSCPDGGLQSPTVANACGVDVAYP
                     TVVEWQNQFDQEIYQTALDTGVPAQLIKNIFSRESQLWPGIYKTYREAGLGQLTENGA
                     DTMLLWNPDFFAQFCPLVLHSSRCELGFGNLTEEEHAMLRGALVTKVNASCTDCPVGI
                     DLTQANFSVRVFSEGLLANCEQVGRIVTNITGQEPRTVSTYVDLWEFTLVNYNAGAGC
                     LTHALNEAWINGEPLDWEHVAPYLEPACQGALDYVSDISQVLSGIEPTPTSWV"
BASE COUNT          666 a          768 c          962 g          634 t
ORIGIN      
        1 atctcggcgg caatttctgt caggctgagc acccgcaccc aaccgcaggc cagctcgatg
       61 cccagcggca ggccttccac cagttcgcaa acgcggatga gggcggcgcg attatcggcg
      121 tttaattcaa aagcggggtc ggcgcgctgg gcgctctgca aaaagagctt gacggcggag
      181 tattcctcaa actcggcatc attgggctgc tgcggatatc gcatcccctg cagcgggtaa
      241 atctgttcgc cgaggaggtt caaacgctcg cgcgaggtga ccaggatttt caaccgggtg
      301 gaacgctgca aaagttgatc aaccaggaca gcgccatcca gcaggtgttc aaaattatcc
      361 attaccagca ggagctgttt ttgaccaagg tattggataa cctgctcatc cagtgtagcc
      421 cggttatgaa aaggaatact aagcgcctgg ccaatcgccg gggcgattaa atcggcagtt
      481 gccagcggcg ccagggggat gaaatagacg ccgtgagcga actgctcaat ggagtcggcg
      541 gcaacctgga gcgccatgcg ggttttgccg gtgccgcccg agccgaccag cgtcagcagg
      601 cggcaggctg gggtttgcag ttgggcaccg atagcagcca ggtcggctgc gcggccaaca
      661 aagggcgtgg tctgcggggg caggttgttg ggatgggcag agagggagcg cagcgccgga
      721 aatttctgat agggcaaatc tggatgatta agctgaagga tgggctgcgg ctgggtcagg
      781 tctttcagca ggtgcattcc cagatcaatc aattcggcgc caggaggcag gggggcctgc
      841 tgggcggcct cgggactgag cagaatctgc ccgccccaac cggcgctaag caggcgctcg
      901 gtacggctga tttcgaggcc gaaaaagtca tcagtgcggg aaatgactgc gccagcatgt
      961 aaagacaccc gcacccgcag ttcgccaatg ggcccccaat tttggctggc gagagattgt
     1021 tgaatcttaa tcgcgcaggt cagcggatcg cctgattcaa aaatggcaaa aaagccatcc
     1081 ccggtgtgtt tgattacccg accaccgtga ttttcgatgg cggaacggac aatctggtcg
     1141 tgggttctaa aggctgcgtc agcctcaacc gggtactttt gccagatttg agtggagtgc
     1201 tcaatatcgg taaacagaat gaccggcagt tcaaatggca taaggctatt atacgttatt
     1261 gcatcaaatg attgtaaaag tatgatacac ttaatttgtt ccattgattt tactggataa
     1321 cgatgagttg tagagaatga atttgatgcg ccgcagggta attctggggg tgttgctgct
     1381 ctccatgatc ctgatgctgg gattgatggg gaatggctat gccattgtgc gcgctgagga
     1441 tggggaagaa acacccgagc cctatgttcg taagaccacg ctgacgctgt catttttaga
     1501 gcatgagtgg tggttggtac gctggaaaga caacgagatt ggctgccggg tctttattga
     1561 gcatgagggt ttgccgagca gtgacgaaat tttgaccgcc tgtggaacca gcctgcataa
     1621 tcaatggctg gcaaccaagc cctgcgcgca ggcagaatcg ggggaagacg tcagcaagtg
     1681 cagcgggctg tatttgcatt acgttgaagc cgtgcccggt gaacgaacgg ttgaagtcga
     1741 gcttccgctg ccgaccgtct ggatatcgct gacgggatgc gaagatatgg atgccgaggg
     1801 gcgctgcgcc aacattccga cgctgctgct ctccggcgaa gagccgctgc cgaacgaaca
     1861 aattatcagc atcgccggga cgattaacgg cgagccgttt agctgtcccg gcagcacctg
     1921 cgaaattccc cttcagccaa ccggttcgca aggggttgag gcgctgttct gggcagaatc
     1981 cagttttgga gactccagcc cggaatacac ggcgctggtg cgggtgcagc cgtggggtga
     2041 ttttatgtcg ccggatggcg gtgggtcaga cccgcagcgc tggtatgctg atgtgctcag
     2101 cacacagtgg cgcggcggac cgctggcaag ctgctcggat atctggcagg tctttccaga
     2161 gatgggcgga ccgccgccgt ggttggatac tccggcacag ccagaagacc tgtattccag
     2221 ctattcgttt tacttcctgg cggggatgtt gatttcaaac ggcgaggtgg acgccagcag
     2281 ctgcccggat ggtgggttac aatcgcctac tgtggcgaat gcctgcgggg tggatgtggc
     2341 ttacccaacc gtggtggaat ggcagaacca gtttgaccag gaaatttatc agactgcgct
     2401 ggatactggc gtgccggcgc aattaatcaa aaatatcttc agccgggaaa gccagctttg
     2461 gccgggcatc tataagacct atcgcgaagc cggactgggg cagctgactg agaatggcgc
     2521 cgacaccatg ctgctctgga acccggattt ctttgcccag ttctgcccgc tggtgctgca
     2581 tagcagccgg tgtgaattag ggtttggcaa cttgaccgaa gaagaacatg ccatgttacg
     2641 cggcgcactg gtgaccaagg tgaacgcaag ctgcaccgac tgcccggttg ggattgacct
     2701 gacccaggca aacttcagtg tgcgggtgtt ctctgaggga ctgctggcaa actgcgagca
     2761 ggtgggacgg attgtgacca acatcaccgg gcaggagccg cgcaccgtca gcacttatgt
     2821 tgatctgtgg gaatttacgc tggtcaatta caacgccgga gccggctgcc tgacccatgc
     2881 attaaatgaa gcctggatca acggcgaacc gctggactgg gagcatgtgg cgccctacct
     2941 ggaacccgcc tgccagggcg cgctggatta tgtcagtgac atcagccagg ttttgagcgg
     3001 gattgagcca acgccgacct cgtgggtgta
//
LOCUS       DAOPVS010000202         3028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_33456_length_3028_cov_53.499159, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000202 DAOPVS010000000
VERSION     DAOPVS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33456_length_3028_cov_53.499159"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..734)
                     /locus_tag="PKL11_10590"
     CDS             complement(231..734)
                     /locus_tag="PKL11_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ14088.1"
                     /translation="MSPSDSTPTPDIHFMHITAKTMNQICKLSETLSPVQHQMVADNV
                     WSIAEAHFSESAWMRAIYADETPIGFIMTHTGSDYNDGIDCPGVFLWRLMIAAPYQGQ
                     GYGRLALEKLFRHLKAMGIPVLYTSCGQGEGSPEGFYRKLGFVPTGDSYDDEIELVLQ
                     LDQYNLP"
     gene            968..2092
                     /locus_tag="PKL11_10595"
     CDS             968..2092
                     /locus_tag="PKL11_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ14089.1"
                     /translation="MSWKRIIYAVLVVALAGVSALGGVVVGGLAVYQALRANADAPQT
                     VFSDASSAPAQVVSVNTTEYQTSVTQAAEAVGPAVVTVVGTIPGQMTFFGPTGDQTVS
                     GSGFFISNEGYILTNHHVIEDVQAIRVILADGSEQSATLVGSDQFSDLAVLKTDGSVP
                     AIVKLGNSDVLKSGESVIAIGSPLGDFKNTVTVGVVSATGRSIDTGEGYQVEDLIQTD
                     AAINQGNSGGPLVNLAGEVIGINTMIVRSSSSGTVAEGLGFAIPVNTARAVAEQIIQK
                     GYFARPKMGISWQFVTPALAARYGLPVAWGAYVTDVQANSPAQRAGLKEGDIITQFDD
                     IPLDDTQPFVNTLFTYNPGDTVTLTVMRGNTEMQVELTLE"
     gene            2295..2729
                     /locus_tag="PKL11_10600"
     CDS             2295..2729
                     /locus_tag="PKL11_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14090.1"
                     /translation="MITLSSVSWFFLVILLVEVSIYTIFAVLVFSKTSGAVCRRWKAS
                     IFSAILLGVLCQAGINGLLLIGLLTKLRLATFIQDLSGYVHLLIPGSWAMLSLITMPT
                     IILATFGIYYDFVKTDDYLIEKYWQNPKIHYGRGQKPPLLKP"
BASE COUNT          677 a          749 c          875 g          727 t
ORIGIN      
        1 tgctgatcgt ctatctgccg atcctgcatg aacccttcaa cacctacgcc ctgcccgcgg
       61 ctgactggtt gattgtgggt ggtttagcca tcaccattgt gcccgtgctg gaaattgcaa
      121 aatggatgga acgcaagggc tggctgggta aagtgagctg aggctcggct gacgatatgc
      181 ttcgggctct gtccattaaa gtgacagagc ccgtttttta tgcctgggga tcagggcaga
      241 ttgtactggt caagctgcag aaccagctca atttcatcat cataggaatc gccggtgggc
      301 acaaacccca acttgcgata aaagccctcc gggctgcctt ctccctgccc acaactggta
      361 tagagcactg gaatgcccat ggctttcaaa tgccggaaca atttttccag ggccagccga
      421 ccgtacccct gaccttgata gggtgcagca atcatcaggc gccagaggaa gacaccgggg
      481 cagtcaatgc cgtcgttgta atccgagcca gtgtgcgtca tgatgaagcc gatgggggtt
      541 tcatcggcat agatggcacg catccaggcg ctttcggaaa aatgggcttc ggcaatcgac
      601 cagacattat ccgccaccat ctgatgctga actggcgaga gggtttcgct cagcttacaa
      661 atctggttca ttgtctttgc cgtgatatgc atgaagtgaa tatcgggggt tggggttgaa
      721 tctgaggggg acatgatttc accttggtca aacaaaaaag tcttgctgga taaattttga
      781 aagctcaaat caactgaact atatcattaa agcagatggc cagaggtgat tttcgggcag
      841 ccagaaaaag ccggggatca gggcagattt aacattttct caggatttct ttggtttttt
      901 cacaggcggg atttctataa tagggtcgaa taagatcaac cattaacacg attatagtga
      961 ggtgactatg tcctggaaac gaattattta tgcggtgctg gtggtggccc tggcgggcgt
     1021 ttcggcgctg gggggtgtgg tggtgggcgg gctggctgtg tatcaggcct tgcgcgccaa
     1081 tgccgatgcg ccccagacgg ttttcagtga tgccagcagc gctccggcgc aggttgtatc
     1141 cgtcaataca acggagtacc agacctcggt tacgcaggcg gctgaggcgg ttggcccggc
     1201 ggtggtcact gtggtgggca ccattcccgg gcagatgacc ttctttgggc cgacaggcga
     1261 tcaaaccgtg agcggcagcg gcttcttcat cagcaatgag ggctatattc tgaccaatca
     1321 ccatgtgatt gaagatgtgc aggctattcg cgtcatcctg gcagatggca gcgagcaaag
     1381 cgccacgctg gtggggtcgg accagttttc tgacctggcg gtgttgaaga cggacggcag
     1441 cgttccggct attgtaaagc tgggcaattc ggatgtcctc aagtcgggtg aatcggtgat
     1501 cgccattggc tcgccgctgg gggacttcaa aaacacggtg acggtgggcg tggtcagcgc
     1561 aaccggacga tccattgaca cgggtgaggg ttaccaggtt gaggatctca tccagacgga
     1621 tgcagcgatc aaccagggca actcgggcgg accgctggtc aatctggcgg gcgaggtgat
     1681 tgggatcaac actatgattg tgcgctccag cagcagcggt accgtggctg aagggctggg
     1741 ctttgccatc ccggtcaata cggcccgggc tgtggctgag cagatcatcc agaagggtta
     1801 ctttgcccga cccaagatgg gcatcagctg gcagtttgtg accccggcgc tggcggcgcg
     1861 ctacgggctg ccggtggcgt ggggggccta tgtgacggat gtgcaggcca acagcccggc
     1921 tcagcgcgcc gggttgaagg aaggcgacat tatcacccag tttgatgaca tcccgctgga
     1981 tgatacccaa ccgtttgtca acacgctgtt cacctacaat cccggtgaca cagttaccct
     2041 gacggtaatg cgcgggaata ccgagatgca ggttgaactc acgctggagt aagcccggca
     2101 aacaaacctg atttcatctt tctcctcctc tgctgccatg cacctgaagc cgaccacgag
     2161 gctttgggtg catggcagca tttcaggaat gggtgaaacc gtggtaaacg ctcagacggc
     2221 gtgaggcctt gccgctcatc aggatgatat ataatcacta caatcatcat ttggattcag
     2281 gattgttggg aaaaatgatc accctttcaa gcgtttcatg gttttttctg gttatcctgt
     2341 tggtggaagt cagcatctat actatctttg ctgttttggt tttttcaaag accagcgggg
     2401 ccgtttgccg gcgctggaag gcctcaatat tttcagctat cctgttgggg gtattgtgtc
     2461 aggccggcat taatggattg ttgttgattg ggctgctcac gaaactgagg ctagcaacct
     2521 ttattcagga tctttccgga tatgtccacc tgctgatacc gggttcttgg gccatgcttt
     2581 cactgatcac aatgcccaca attattctgg caacctttgg gatttattat gattttgtta
     2641 agactgacga ttacctcatt gaaaaatact ggcaaaaccc aaaaattcat tatggccgcg
     2701 gacaaaaacc gccgctcctc aaaccataac caacgtcccc gagcaagaaa accaagggaa
     2761 atcgacagtc tagtgattgg caaaccatca tgccgcttaa gccgcggctt cggcaggaaa
     2821 caggttcgct gatgcaactt tctggggcga cgggcgtata ataggcgtca cgtctgacaa
     2881 tttgtcctga aaggcatcac tatggaaaca ccaccctcaa acccgaccgt gccagaacgc
     2941 tgggcgccgc gcttttttac catttgggca gggcaggctt tttcgctgat tggttcagcg
     3001 ctggtgcagt ttgccctggt gtggtgga
//
LOCUS       DAOPVS010000203         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_33762_length_3010_cov_69.672081, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000203 DAOPVS010000000
VERSION     DAOPVS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33762_length_3010_cov_69.672081"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKL11_10605"
     CDS             <1..636
                     /locus_tag="PKL11_10605"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNZ14091.1"
                     /translation="DMFYLLMSGLVAMPSGNDLISVRQRVRQSGRANLWVLPGSKETA
                     MAQTTVVALLKPINYLADLLRIFTCGENGRSSGLDAVILDTGPSLGGWLQEAAIWAAD
                     LVIAPVTPDFPSADGLRQFVETLRSLNAKGWTGKLAGVLPTMIDDRTRETRTIMGEIE
                     QTFGSLVLPGIHDATVFRECAAEGETIFEKAPVSRAAREYQTVVDAVARML"
     gene            657..1286
                     /locus_tag="PKL11_10610"
     CDS             657..1286
                     /locus_tag="PKL11_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14092.1"
                     /translation="MARRKPFETPQPTEPTPAPLAASPSATLDALAYLRLPEKKKRQR
                     SWEREHTPKSYRGVPADLRDAVRAAAENLNVTVDDLARAFLEYSLDCVSRGALQLEIR
                     YSPRVQRLSLYPFSGASWAARGWNPQPPPAPARRRKAAENARPWQQLVSYRIPDELHQ
                     QIKSQAGSDVPLGEVVTILLQHALTDYRSGVLALFPQPRMAAKVHWESA"
     gene            1556..2554
                     /locus_tag="PKL11_10615"
     CDS             1556..2554
                     /locus_tag="PKL11_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14093.1"
                     /translation="MNDDFGPTIKGVTIPETVILPPEVIQLLPWMSLAELKITIAAVA
                     RLMQVGGAEPITLSEFEMLTGLARASVVEGIERAMQRGLLTRFEISGYQGHLAYVYEI
                     QPRFIGSKIEPMKPVKDKPRQAVVDESESTTNLNLTGAAAENLTADPGEARRNNLYAR
                     LRKIGIYPKTAANLLRSNDPARVEQFLGLYPLAVQAGRAAGPGWLVTAVTDASWDPAV
                     EQADLEARLADRRTRQAEPAEAPAPAQGLPQKVLAALRQIGWNGDTAEVLEAYTRSKK
                     LVRAWLEWAATQPPEYQAARFRNGLRSGQMPPASGKRRPASSRRYVAGKYGEYINH"
     gene            2581..>3010
                     /locus_tag="PKL11_10620"
     CDS             2581..>3010
                     /locus_tag="PKL11_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14094.1"
                     /translation="MPNFTNRVHLQGFLEIDRAAIRSLGDQVCSPVLWGWLHSGTCET
                     AERHRIVISDRPAVATLEILRRVSASPQPEIMLTASLDGAVEAVALNGRPLVSIEGAL
                     LSVPGKPAVVDVKWISFLTVALNLPPDGDDYATVRSLGRVW"
BASE COUNT          569 a          954 c          975 g          512 t
ORIGIN      
        1 gacatgtttt acctgctgat gtccgggctg gttgcgatgc ccagcggcaa cgacctgatc
       61 agcgttcgcc agcgggtgcg ccagtctggg cgggccaatt tatgggtgct gccgggcagc
      121 aaagagaccg ccatggcgca gaccaccgtg gtggccctgc tgaagccgat caactacctg
      181 gctgacctgc tgcggatttt tacctgcggt gaaaacgggc gctccagcgg gctggatgcc
      241 gtgatcctgg ataccggccc cagcctgggc ggctggctgc aggaagcggc catctgggcc
      301 gccgacctgg tgattgcacc ggtgacgccc gactttcctt ccgccgatgg gctgcggcag
      361 tttgttgaga ccctgcgcag cctgaacgcc aaaggctgga ccggcaaact ggccggggtg
      421 ctgcccacca tgattgacga ccgcacccgt gagacgcgga ccattatggg cgagattgag
      481 cagaccttcg gcagcctggt gctgcccggg atccatgatg ccaccgtgtt ccgggaatgc
      541 gccgctgagg gcgaaaccat ttttgagaag gcgccggtga gccgcgccgc ccgcgaatac
      601 cagacggtgg tggacgccgt cgcccggatg ctttagccga atggaggtga atggttatgg
      661 cgcgccgcaa accctttgaa accccacagc ccacagaacc cacacccgca cccctggctg
      721 cttcgccatc ggcgaccctg gacgccctgg cttacctgcg cctgccggaa aagaaaaagc
      781 gccagcgcag ttgggagcgg gagcataccc ccaagagcta ccgcggcgtt cccgctgacc
      841 tgcgggatgc cgtgcgagcc gcagccgaaa acctgaacgt gacggtggat gacctggccc
      901 gggcctttct ggaatacagc ctggactgcg tctcccgggg agcgctgcag ttggagatcc
      961 gctacagccc gcgggtccag cggctctcgc tgtatccgtt ctcgggcgcg tcctgggctg
     1021 cccggggctg gaacccccag ccgcccccgg cgccagcccg gcgcaggaaa gccgccgaga
     1081 atgcccgacc ctggcagcag ctcgtcagct accgcatccc tgatgaactg caccagcaga
     1141 tcaagtccca ggccggaagc gatgttccgc tgggcgaagt ggtcaccatc ctgctgcagc
     1201 acgccctgac ggactaccgc tccggcgtcc tggcgctgtt cccgcagccc cgcatggcgg
     1261 ccaaagtgca ctgggagtca gcatgagcaa acaccgcaca gcccggtttc gagaaaaaca
     1321 gatggatttt ttaccggtca aaatgaacgt aagcggcatt gcaaacgtgc cgcttacgcg
     1381 ccgcaaacgt gccgcttacg ctgccgcaaa cgctgccgca aacgttgcgc cttcaggatg
     1441 ccctgtgagg cgtgttttga aaccaggatg agtattgata tccccggaaa tttgaacccg
     1501 tctgcgagcc tctcaggggt ttgcggggga aggctgtcag aaaaggattt tgcgcatgaa
     1561 cgatgatttt ggaccgacca tcaagggggt gaccatcccc gaaaccgtaa tcctcccgcc
     1621 ggaggtgatc cagctgctgc cctggatgag cctggcggag ttgaagatca ccattgccgc
     1681 ggtggcccgg ctgatgcagg tgggcggcgc tgagccaatc acactgagcg agtttgagat
     1741 gctcaccggg ctggcccggg catcggtggt tgagggcatt gagcgcgcca tgcagcgcgg
     1801 cctgctgacc cggtttgaga tcagcggcta ccaggggcac ctggcctatg tgtacgaaat
     1861 tcagcctcgt ttcatcggtt caaaaattga accgatgaaa ccagttaaag acaagccaag
     1921 acaagctgtt gttgatgaat ctgaatcaac aacaaactta aacttaactg gcgccgccgc
     1981 tgaaaatttg accgccgacc cgggagaagc ccgccgaaac aacctgtatg cccgcctgcg
     2041 caaaattggc atctacccca aaaccgcggc caatttgctg cgcagcaacg acccggcccg
     2101 ggtggagcag ttcctgggcc tgtatccgct ggcagtgcag gccggacgcg ccgctggccc
     2161 cggctggctg gtgacggcgg tgaccgatgc aagctgggac ccggccgttg agcaggccga
     2221 cctggaagcg cgcctggcgg accggcgaac ccggcaggct gaaccggccg aggcccctgc
     2281 cccggcgcag ggactgcccc agaaggtgct ggcggccctg cgccagattg gctggaacgg
     2341 cgacaccgcc gaggtgctgg aagcctacac ccgcagcaaa aagctggtgc gcgcctggct
     2401 ggaatgggct gccacccagc cgccggagta ccaggccgcc cgcttccgca acgggctgcg
     2461 ctccgggcag atgccgcccg ccagcggcaa gcgccgaccc gccagctcgc gccggtatgt
     2521 tgcgggtaag tacggcgagt atatcaacca ctaaccccac gttttgagcg aaaggaaatc
     2581 atgccaaatt ttaccaatcg ggttcacctg cagggttttc tggaaattga ccgggccgcc
     2641 atccgctccc tgggcgacca ggtctgctcg ccggttttgt ggggctggct gcactccggc
     2701 acctgtgaga cggctgaacg ccaccggatt gtgatcagtg accggccagc ggtggccacg
     2761 ctggagatcc tgcgccgggt ctcggccagc ccgcagccag agattatgct gaccgccagc
     2821 ctggacggcg ccgtggaggc cgttgcgctg aacggcaggc cgctggtgag cattgagggc
     2881 gcgctgctgt cggttccggg caagcccgcg gtggtggatg tgaagtggat ttctttcctg
     2941 acggtggcgc tgaacctgcc gccagacggc gatgactatg ccacagtgcg ctcgctgggc
     3001 cgggtgtggg
//
LOCUS       DAOPVS010000204         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_34489_length_2966_cov_35.595328, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000204 DAOPVS010000000
VERSION     DAOPVS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34489_length_2966_cov_35.595328"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /gene="glmS"
                     /locus_tag="PKL11_10625"
     CDS             <1..1760
                     /gene="glmS"
                     /locus_tag="PKL11_10625"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNZ14095.1"
                     /translation="YDSAGIAVIEAGHIQVRREAGKLSKLSALVDAQPLQGSIGVGHT
                     RWATHGEPTARNAHPHIGSTGNVVLVHNGIVENYLELRADLAADGVQFNSDTDTEVIV
                     HLIERFLSTHTSLEEAVRQALSQIKGAHGVVVLSTLEPDKMIAARIGNAGGVVIGLGE
                     DENFIASDIPAILDHTRRMMFLESHQMAVVTRSSVTVKTLSGRKVTLEEHQIGWDLVS
                     AEKGEYRHFMQKEIHEQVRSLTDTLAGRVDFDEGTIRLPELNLIPELARRINKVIITA
                     CGTAAHAGMVGKVLIEHIARVPVEVDIASEFRYRNPIVDENTAILAISQSGETADTLA
                     AMDEARRRGAIIWSVVNAIGSQAMRVADGYISMQSGPEIGVASTKAFTAPLVDLYMLA
                     VLLGELRGALPDRERRKLVSDLRLIPDLVGRCLDREAEIVQAARELRYMRNILYLGRG
                     INMPIAYEGALKLKEISYIHAEAYAAGEMKHGPIALIDRDLPVLAIAPQDPWHEKMVS
                     QIEQARARGGNVLVVATEGDERVPPLADHVFWVPETPWLLSPVVTVIPLQLFAYHIAA
                     LKGLDVDQPRNLAKSVTVE"
     gene            1832..>2966
                     /locus_tag="PKL11_10630"
     CDS             1832..>2966
                     /locus_tag="PKL11_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14096.1"
                     /translation="MTLLSRLKSLRFSDKSIPWALLIACILSYGLLIPWLGFYWDDWP
                     YILLYRTHGPLAYIPFLASDRPFSPLIHVLISSVIGDWQLGYHLLMLVIRWLAAVAFW
                     WALRAIWPQHARRLVWAPFLFAVYPGFSQQPLAVIYTLHWPNMIISILSLGWMALALR
                     HPASRRWLTLLSLTGPLSLLVMEYFFGLEILRPLLIWFVLAEQNLPPRQRLIKTLRAW
                     LPYALILTVFMVWRVAVFQFPTYSPVVLDKLQASPLLQGWRFIETALRETFVAGVQAW
                     LQPLSALGGLEIHNRLDVLYWALIVLGALAACLYLLRLKTPLSDQPTQPIQGWPVQPL
                     IAALVALFAAGLPFWATDLSVGLYFPNDRFTLPFIFGASLLAGT"
BASE COUNT          543 a          899 c          783 g          741 t
ORIGIN      
        1 gttatgattc cgctggcatt gccgtgattg aagcggggca tattcaggtt cggcgtgaag
       61 ccggtaaatt gagcaaactt tccgccctgg tggatgcaca acctttgcag ggttccatcg
      121 gcgtagggca tacccgctgg gcaacccatg gcgaacctac cgcccgcaac gctcaccctc
      181 acattggcag caccgggaat gttgtcctgg ttcacaatgg cattgtcgaa aactacctcg
      241 aactgcgcgc tgatttggcg gctgatggcg tgcaattcaa ctcggatacc gataccgagg
      301 tcattgtcca cctgattgaa cgcttcttat caacccatac atcgcttgaa gaagcggtcc
      361 gccaggccct cagtcaaatc aagggagcgc atggtgtcgt ggttctttcc actcttgagc
      421 ctgacaaaat gattgccgcc cgcattggca atgcaggtgg cgtggtgatt ggcctcggtg
      481 aagacgaaaa cttcatcgcc tccgatatcc ccgctattct ggatcacacc cgccgcatga
      541 tgttcctcga atctcaccag atggcagttg ttacccgcag cagcgtaacc gtcaaaaccc
      601 tttctggacg caaagtcacc ctcgaagaac atcaaatcgg atgggacctc gtctcggccg
      661 aaaaaggtga atatcgccac tttatgcaga aagaaatcca cgagcaggtc cgctcattga
      721 ccgatacact ggctggccgc gtggattttg acgaaggcac catccgcctg cccgaattaa
      781 acctgatccc cgaacttgct cgccgtatca acaaagtcat catcacggct tgtggcactg
      841 ctgcccacgc cgggatggtt ggcaaagtgc tgattgagca cattgcacgt gttcccgtag
      901 aggtggatat tgcttcagaa tttcggtatc gcaatccgat cgtggacgag aacactgcaa
      961 tccttgcaat cagccaatcc ggtgagactg ccgataccct tgccgccatg gacgaagccc
     1021 gccgccgcgg cgccatcatc tggtcagtgg tcaacgccat tggctctcag gccatgcgcg
     1081 tggctgatgg ctacatctcg atgcagagcg gccccgagat cggtgtggcc tctaccaagg
     1141 cgttcaccgc gccgctggta gatctgtata tgctcgccgt tttgctgggc gagctgcgcg
     1201 gagcgcttcc cgaccgcgag cgccgcaagc tggtgtcaga cctgcggttg atccccgatc
     1261 tggtgggtcg ctgcctggac cgcgaggccg aaatcgttca ggctgcccgc gagctgcgct
     1321 atatgcgcaa tatcctttat ctggggcggg ggattaacat gcccattgcc tatgaaggcg
     1381 ccctcaagct caaggaaatt tcgtatatcc atgcggaggc ctacgccgcc ggcgaaatga
     1441 agcacggccc gattgccctc attgaccgtg acttgcctgt tttagccatt gccccacagg
     1501 acccctggca tgaaaaaatg gtcagccaga ttgagcaggc ccgtgcccgc ggggggaacg
     1561 ttctcgtggt tgccaccgaa ggcgacgagc gcgtcccacc cctggccgat catgtcttct
     1621 gggttcccga aacaccctgg ctgttaagcc ccgtggtcac ggttatccca ctgcaattgt
     1681 ttgcctatca cattgccgcc ctcaaagggt tagatgtgga ccagccgcgc aatctggcga
     1741 aatccgtcac cgtagaataa agcccggtca ttagaattgc ggtatactga gggctgggtt
     1801 tggcagtctc agccctcagt tctggtgttt tatgaccctc ctctctcgcc tgaaatcact
     1861 acgcttctct gataaatcca tcccctgggc actgctgatt gcctgtatcc tcagctatgg
     1921 tcttctcatt ccctggctgg gcttctattg ggacgactgg ccttatatcc tgttgtatcg
     1981 tacccatggg ccgctggcct atatcccttt cctggcctca gaccggccct tctcgccatt
     2041 gattcatgtt ctcatctcgt ctgttattgg cgattggcag ttaggctacc acctgttgat
     2101 gctggtaatc cgctggctgg cagccgtggc tttctggtgg gcgctgcgcg ccatctggcc
     2161 tcagcatgcc cgccgcctgg tctgggcgcc attcctgttt gccgtttatc ctggttttag
     2221 ccaacagccc ctggctgtca tttataccct ccattggccg aatatgatca tcagcatcct
     2281 atcgctgggt tggatggccc tggctttacg ccaccctgcc agccgtcgct ggttgactct
     2341 cctgtcgttg accggcccac tcagtttact ggttatggaa tatttctttg gcctggagat
     2401 tttgcgcccc ctgctcatct ggttcgtcct tgcagagcag aatcttcccc cgcggcagcg
     2461 gctgattaaa actctgcgcg cctggctgcc ctacgccttg atactgactg tctttatggt
     2521 ctggcgggtg gcggttttcc aattccctac ctattcccct gttgtattgg ataaacttca
     2581 ggcttccccg ctccttcagg gctggcgctt tatcgaaact gccctgcgcg agacgtttgt
     2641 tgccggtgtg caggcctggc ttcagccatt atcggcgctc ggcggattgg aaattcacaa
     2701 ccgattggat gtcctctact gggctctgat tgtcctgggc gctctggcag cctgccttta
     2761 ccttctgcgt ctcaaaaccc cactatcaga ccagcccacg cagccgattc aaggctggcc
     2821 tgtccagccc ttgatcgccg ctctggtagc tttgtttgct gccgggctgc cgttttgggc
     2881 aaccgacctt tctgtggggt tgtattttcc caatgaccgc ttcacgctgc cctttatctt
     2941 tggcgccagt ctgctggcgg gcacgc
//
LOCUS       DAOPVS010000205         2952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_34710_length_2952_cov_50.925440, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000205 DAOPVS010000000
VERSION     DAOPVS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34710_length_2952_cov_50.925440"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1089
                     /locus_tag="PKL11_10635"
     CDS             61..1089
                     /locus_tag="PKL11_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ14097.1"
                     /translation="MNIAVLVQWLATFAWIGLVGFFVYLLVRAAQHRPIPRGGLGIMI
                     IGVVAVILTTVASGLVFIKPDERGVVISAVAPGGYRPLPLEPGLRWVIPFAESVVRYP
                     ISRQTYTMSIASFEGDISGDDSIEARTADGQKIYVDASAIYSINPAEVVNVHILWNDR
                     YTDGLVRPLARGAIRDAVSQYRVEEVVTTKRDEVVAAISEAMSTSLEENGIIMVDFIL
                     RNITYTDEYAASVEQKQIAEQLAQQAVYVVEQRKQEAEQARQVAQGQADAAVIAAQGA
                     ADARIIQAEAEARALELIADALKDRPELLTYQYITKISPNVEVMFLPNNSPFIFPLPE
                     TQTIIPAE"
     gene            1144..2205
                     /gene="selD"
                     /locus_tag="PKL11_10640"
     CDS             1144..2205
                     /gene="selD"
                     /locus_tag="PKL11_10640"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1207..1209,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNZ14098.1"
                     /translation="MNNHSPSGKQIRLTSLSNCAGUASKLNAETLAHVLRPLSQTFDP
                     AAYPDLLVGLQSPDDAAVWRLDESRALVLTTDFFTPVVDEPYDYGAIAAANSLSDIYA
                     MGGTPVLALNIAALPPDLPPELSSEILRGGAEKCRAAGVVIAGGHTVTDKEPKYGLVA
                     LGFVDPRQMLTKAGARPGDRLLLSKPLGFGVTTTALKRQQAAEADVAEAVGWMQQLNR
                     AAGELAVAHGLRAATDITGYSLLGHGWEMASASGAGLRLFYDQIPFISCARRYAEAWT
                     FPGGASDNALFYTPHVRFAPRLDEAAQMLLFDPQTSGGLLLAVPPERLSALQAQAAER
                     QQPLWEIGEVIPEEVIEVV"
     gene            2192..2872
                     /locus_tag="PKL11_10645"
     CDS             2192..2872
                     /locus_tag="PKL11_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNZ14099.1"
                     /translation="MKWSEIEYPWQVCFEEAWHAYCAGSLPIGAALFSPEGQLLFRGR
                     NHINDTEPDGRIVKGDLAHAEINVLLQINRETLTPRHCTLYTLLEPCPLCLGAFYMSG
                     VRHLSYAARDTFAGSTNLLGKTPYLSRKKIYLTGPISLLEPAIVALQVAAHLRRGFDR
                     QGWADFYAEWEATSPEGMALGVQLYESRRLQAAADAAQPISQVYADIGTALAEKSLES
                     SSSPQLET"
BASE COUNT          535 a          970 c          871 g          576 t
ORIGIN      
        1 cccccattta tggtatatta atcctgttcg attctttcat ctgttgtcag gagcaacccc
       61 atgaatatcg cggtcttggt tcaatggtta gccaccttcg cctggattgg cctggtgggc
      121 tttttcgttt atctgctggt gcgtgcggcg cagcaccgcc ccatcccgcg cggcggcctg
      181 ggcattatga ttatcggcgt ggtggcggtg attctcacca cggtggcctc ggggctggtc
      241 ttcatcaagc ccgatgagcg cggcgtggtc atttcggcgg tggcgccggg cggctaccgc
      301 cccctgccgc tggaacccgg cctgcgctgg gtcatcccgt ttgccgaatc ggtagtgcgt
      361 taccccatct cgcgccagac ctacaccatg tccatcgcct ctttcgaagg cgatatctca
      421 ggtgacgact cgattgaggc ccggactgcc gatgggcaaa aaatttatgt ggatgcctcg
      481 gccatctatt ccattaaccc ggcagaggtg gtcaatgtgc atatcctgtg gaacgaccgc
      541 tataccgatg ggctggtgcg cccgctggcc cgcggcgcta tccgcgatgc tgtctcgcag
      601 taccgcgtgg aagaggtggt tactaccaaa cgtgatgagg tcgtagccgc catctctgag
      661 gcgatgtcca ccagcctgga agaaaatggc atcatcatgg ttgattttat cctgcgcaat
      721 atcacctata ccgatgaata tgcggcctca gtggagcaga agcagattgc cgagcagttg
      781 gcgcagcagg ccgtctatgt ggtggaacaa cgcaagcagg aggctgagca ggcccgccag
      841 gtggcccagg gtcaggctga tgctgccgtc atcgccgccc agggtgcggc cgatgcccgc
      901 atcatccagg cggaggccga agcccgcgcc ctcgagctga ttgccgacgc cctcaaagac
      961 cggcccgagc tgctcaccta tcaatacatc accaaaatca gcccaaatgt tgaggtgatg
     1021 ttcttgccca acaattcacc cttcatcttc cccctgcccg aaacacaaac catcattccg
     1081 gcagagtaaa aaccgtcttg ccgccgggga cgagccatcg tccccggctt tagcatcagg
     1141 cctatgaaca atcactcccc ttccggcaaa cagatccgcc tcaccagcct ctccaactgt
     1201 gcgggttgag cgtctaaact caatgcggaa acgctggcgc acgtgctgcg ccccctgagc
     1261 cagacctttg accccgccgc ctaccccgac ctgctggtgg gcctgcagtc gcccgacgat
     1321 gccgccgtct ggcggctgga tgaatcgcgc gccctggtgc tgaccaccga cttcttcacc
     1381 cccgtggtgg atgaacccta tgattacggc gccatcgccg cggccaacag cctgtccgat
     1441 atttacgcca tgggcggcac gcccgtgctg gcgttgaata ttgccgccct gccgcccgat
     1501 ctgccgcctg aactatcctc agaaattttg cgcggcggcg ctgaaaaatg ccgcgcggcc
     1561 ggggtggtaa ttgccggcgg acataccgtg accgataaag agcccaaata cggcctggtg
     1621 gcgctgggtt ttgttgaccc ccggcagatg ctcaccaaag ccggggcgcg ccccggcgac
     1681 cggctgctgc tcagcaagcc gctgggcttt ggcgtgacca ccactgcgct caagcgccag
     1741 caggccgccg aggccgatgt ggccgaggcg gtgggctgga tgcagcagct caaccgggcg
     1801 gctggcgagc tggcggtggc gcacggactg cgcgccgcca ccgatatcac cggctacagc
     1861 ctgctgggcc acggctggga aatggcctcg gccagcggcg ccggactgcg cctgttctac
     1921 gaccagatcc cgttcatctc ctgcgcccgg cgctatgccg aggcctggac cttccccggc
     1981 ggggccagcg acaatgccct gttctacacg ccgcacgtgc gttttgcccc ccggctggat
     2041 gaggctgccc agatgctgct ctttgatccc cagaccagcg gcgggctgct gctggcggtg
     2101 ccgccagagc ggctgagcgc cctgcaggcc caggctgccg aacgccagca gcccttgtgg
     2161 gagattggcg aagtcatccc ggaggaagtc attgaagtgg tctgaaatag aatatccctg
     2221 gcaggtgtgc tttgaggaag cctggcacgc ctactgcgcc ggctcgctgc caattggcgc
     2281 ggccctgttc agcccggaag gccaactgct ctttcgcggg cgcaatcaca tcaacgacac
     2341 cgaacccgat ggacgcattg tcaagggtga cctggcccat gccgaaatca acgttttgct
     2401 gcaaatcaac cgcgaaacgc tcaccccgcg ccactgcacg ctttataccc tgctggagcc
     2461 ctgcccgctg tgcctgggcg ccttttatat gagcggtgtg cgccacctca gctacgccgc
     2521 ccgcgatacc tttgcggggt ccaccaattt gctgggcaaa acgccctacc tctcgcgcaa
     2581 gaaaatttat ctcaccggtc ccatcagctt gctggagcct gcgattgtgg cgctgcaggt
     2641 ggcggcgcac ctgcgccgcg gctttgaccg ccagggctgg gccgattttt acgccgaatg
     2701 ggaggccacc tcgccagagg gcatggccct gggcgtgcag ctctacgaga gcaggcggct
     2761 gcaagctgcc gccgatgccg cccagcccat cagccaggtc tatgcagaca ttggcacggc
     2821 gctggcggaa aaatcattgg aatcatccag ttcaccccaa cttgaaacgt gaaacatgaa
     2881 acctgaaaca tttgctcctg accccctcga attttcttcc cgccgctcgc cggtgtatgg
     2941 gcgcgggggc at
//
LOCUS       DAOPVS010000206         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_34845_length_2943_cov_51.616690, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000206 DAOPVS010000000
VERSION     DAOPVS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34845_length_2943_cov_51.616690"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..2758)
                     /gene="mutS"
                     /locus_tag="PKL11_10650"
     CDS             complement(194..2758)
                     /gene="mutS"
                     /locus_tag="PKL11_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ14100.1"
                     /translation="MTTDDVTPIRQQYLEIKRQYPDAILFFRLGDFYETFDQDAEITS
                     RELDIVLTSRNVAKGARVPMAGIPYHAVENYLARLIDKGYHVAICEQVGDQPTRGLFP
                     RQVVRVVTPGTLIEPGLLHSDRNNYLACVVMLDERAGIAYADITTGEFGVTELEGAEL
                     LNPLRAELMRLNAAEVLMPETLALPDGWPGHVTRWPGWRFEPGRCQEALLRQFQAGSL
                     DGFGLRGMTLAIRAAGAIVQYLQETQAAALKLLTGLATYSLDEFMTLDAPTRRNLELT
                     ETIRTGEVQGSLLGVLDYTVTPMGKRLIRQWVSKPLLNLAAIDQRQNGVQTFVDDGML
                     RAELRTSLRGLNDLERLTNRVLSGHATPRDLTALRATLRALPNIRALLPEGGPLQGLM
                     QALNPCEEELGLLEAAISDEPPATLQNTGIIRPGYSAELDHIVEASRHAREWIANLEA
                     VERERTGIKTLKVGYNKVFGYYIEITRANADMAPSDYIRKQTLVNAERYITPEMKEYE
                     ALVLNAEDRIREVETRLFREICAALGAGSTRLLGSARALAALDTLAALAEAAAIGGYT
                     RPRLANDETLEIHEGRHPVVERLLSGGTRFVPNDAIFENGEVVRLITGPNMSGKSTFL
                     RQAALIVLMAQIGSFVPAASATIGLVDRIFTRIGAQDEIHAGQSTFMVEMIEAANILH
                     HATPRSLLILDEIGRGTSTYDGVSIAWAVVEYIHNHPRLRARTLFATHYHELTQLSDL
                     LPGVRNYNVAVSESEGQVVFLHRIVPGGADRSYGIHVAQLAGLPRSVILRAGEIMAQL
                     EASSGRAVKINPEAAQQMALFPETNPLLDELRALDMNTVSPIEALNKLYEWQKKYL"
BASE COUNT          604 a          969 c          709 g          661 t
ORIGIN      
        1 cggtctgggt ttgacttcag ttctgctccc ggctgcggcc ttactgggtt atgccatact
       61 gttccttggt ctgtccacct ggcgcttcca ccgcagtgca agtgcttgaa aacttaaggg
      121 ttcggcattc aattgagcgt cgaaccctta agttttgctt caaatgatcg aaaacctgac
      181 cgagtagctg tggctagagg tatttctttt gccactcata cagcttgttc aaagcctcaa
      241 ttggcgaaac ggtattcata tccagcgctc tcaactcatc cagcagcggg ttggtttccg
      301 ggaacaaggc catctgttgc gccgcttccg ggttgatctt cacagctcgc cccgaagagg
      361 cttctaactg tgccataatc tcgcccgccc gcagaatcac cgaccgcggc agccccgcca
      421 actgcgccac atgaatccca taagatcggt cggccccgcc cggaacaatc cgatgcagga
      481 ataccacctg cccttcagat tcgctgaccg ccacattgta attccgcacc cccggcagca
      541 ggtcgctgag ctgcgtcagt tcgtggtaat gcgtggcaaa cagcgtgcgc gcccgcagcc
      601 gcgggtgatt gtggatatac tccaccactg cccacgcaat cgaaacgcca tcataagtgc
      661 tcgtcccgcg tccgatctcg tccaggatga gcaggctgcg cggcgtggca tgatgcagaa
      721 tattagccgc ctcaatcatc tcaaccataa aggtggactg ccccgcgtga atttcatcct
      781 gcgccccaat tcgggtaaaa atccggtcca ccagcccaat ggtggctgaa gctgccggca
      841 caaaactgcc aatctgcgcc atcaacacaa tcagcgccgc ctgccgcagg aaggtgcttt
      901 taccgctcat attcggcccg gtaatcaacc gcaccacttc gccgttctca aagatggcat
      961 cattgggcac aaaccgcgtg cctccgctca atagccgttc aaccactgga tgccgccctt
     1021 cgtgaatttc cagcgtttca tcgtttgcca gcctgggtcg ggtgtacccg ccaatcgctg
     1081 ccgcttctgc cagtgctgcc agcgtatcca gtgctgccag tgcccgtgcc gagcccagca
     1141 gccgggtgct gccagccccc agcgccgcac agatctcgcg gaacaaccgg gtttcaacct
     1201 cgcggatgcg gtcttccgcg ttcaacacca gcgcctcata ctccttcatt tccggtgtaa
     1261 tatagcgttc tgcattcacc agcgtctgct tgcggatgta atcactgggt gccatgtctg
     1321 cgtttgcgcg tgtaatttca atataatagc caaaaacttt gttgtaccca acttttagcg
     1381 ttttgattcc tgtccgctcg cgctccaccg cctccaggtt cgcaatccat tcgcgcgcgt
     1441 gccggctggc ttccacaata tggtccagct ctgccgaata tcccgggcgg atgatgcccg
     1501 tgttttgcaa tgtggctggc ggctcatcgc tgatggccgc ttccagcagc cccagttcct
     1561 cttcacaagg gttcaatgcc tgcatcaacc cctgcagcgg cccgccttca ggcaataatg
     1621 cccggatgtt gggcagtgcc cgcagtgtcg cccgcagtgc agtcaggtcg cgtggggtgg
     1681 cgtgtccgct caatacccgg ttcgtcagcc gctccaggtc attcaacccc cgcagactgg
     1741 tgcgcagttc agctcgcagc atcccatcat ccacaaaagt ttgaacgcca ttttggcgtt
     1801 gatcaatcgc tgccaggttc agcagcggct tgctcaccca ctgccggatt aatcgcttgc
     1861 ccatcggggt caccgtataa tccagcaccc ccagcagcga tccctgaacc tcaccggttc
     1921 gaatggtttc ggtcaattcc agattgcgcc gggtcggcgc atccagcgtc ataaactcat
     1981 ccaggctgta ggttgccagc ccggtcagta gcttgagtgc agccgcctgg gtctcctgca
     2041 ggtattgcac aattgcccct gccgctcgaa tagccagcgt cattccgcgc agtccaaacc
     2101 catccagcga tcctgcctga aactgccgca gcagggcttc ctggcaccgc cccggttcaa
     2161 accgccaccc cggccagcgg gtcacgtgcc ccggccagcc atctggcagc gccagtgtct
     2221 cgggcatcag cacctcggcc gcattcaatc gcatcagttc cgcccgcagc ggattcaata
     2281 actcggcgcc ctccagttct gtgacgccaa actctcccgt ggtaatgtcg gcatacgcaa
     2341 tcccggcgcg ctcatccagc atgaccacac aggccagata gttattgcgg tcgctgtgca
     2401 gcagccctgg ctcgatcagc gtgccggggg taaccacccg caccacctgc cgcgggaaca
     2461 acccccgcgt gggctggtct cccacctgct cgcaaatcgc cacatggtat cccttatcaa
     2521 tcagccgcgc tagataattt tccaccgcat ggtatggaat ccctgccatc ggcacccgcg
     2581 cccccttggc cacattgcgt gaggtcagca caatatccaa ctctcggctg gtaatttctg
     2641 catcctggtc aaaggtctca taaaaatcac ccagccggaa gaacaaaatc gcatccggat
     2701 actgccgctt gatttctaga tattgttgcc gaatcggggt tacatcatca gttgtcatag
     2761 atagtccagt ataataattt gagtaacttt gtgaaaaaac cgttggcctc agaaagccct
     2821 ttaaccgatc ctaaatccta aatcccaaat cctaaatcct aaatcccaaa tcctaaatcc
     2881 taaatcttaa atctttcacg gccacttaac ttcccgcagc ccgcttgcgc tattgaatgc
     2941 gta
//
LOCUS       DAOPVS010000207         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_36003_length_2874_cov_38.473572, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000207 DAOPVS010000000
VERSION     DAOPVS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36003_length_2874_cov_38.473572"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKL11_10655"
     CDS             <1..191
                     /locus_tag="PKL11_10655"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNZ14101.1"
                     /translation="VAIHSTYWHNNYGEPMSHGCVNVRPDDARFVYRWSLPQSPYDDG
                     KIEVSGYENVTRVIVREE"
     gene            196..918
                     /locus_tag="PKL11_10660"
     CDS             196..918
                     /locus_tag="PKL11_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNZ14102.1"
                     /translation="MAADVTIGLAFLAGLASFLSPCVLALVPAYVSYLSGRAIRSNQA
                     DAQPDRWLTLWHGFAFVLGFSVVFILIGLAASALGALLYDIRNWLARLGGLIVLLFGL
                     HLTGLLRLPFLEYDLRPQSRLDRQGSFVSSFLMGIVFSAGWSPCTGPILGAIATLAMN
                     TANLPLGALLLTAYSLGLAVPFLAAALSIGWVTTALRRYQKLLHFIEIAMGIILIVVG
                     VMMLLGIFQQLALLAPGVDFGL"
     gene            928..1956
                     /locus_tag="PKL11_10665"
     CDS             928..1956
                     /locus_tag="PKL11_10665"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNZ14103.1"
                     /translation="MTITATLYYKPGDEDRTFEIKQTLADLQASMPHELILMDGTKYP
                     AVGVEPDTQLPVLQIGPYQLKGQFTPQEVEVALRAAQDRESALQRIDGSALASTGITV
                     TNGDRLSLWLSKHYMWVIILSLAVYAGLPFLAPVLMKTGAAAPARIIYKIYTPLCHQL
                     AFRSWFLFGEQSFYPSELAEMDNVISYEAWIGKEGIRLSEDPSTSNAFIIQARDTLGD
                     ERMGYKVALCQRDVAIYGSMMLFGILFAITGRRWKGLSWKWWLIIGIVPIALDGGSQL
                     PGLLSWLPFSLPIRESTPFLRTLTGTLFGIMTAWYLFPVIEESMRETRQMVDWKYAQA
                     RRTREVVS"
     gene            1956..2732
                     /gene="pgeF"
                     /locus_tag="PKL11_10670"
     CDS             1956..2732
                     /gene="pgeF"
                     /locus_tag="PKL11_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNZ14104.1"
                     /translation="MAWRDYGELRAYEFETLPVLHGFFTRRGGVSPAPWESLNHGGTV
                     GDIRANVVENRRRVFAAIGRPVESIFDVWQVHSATVVVTDAPRPLDQPHQSADAIITN
                     NPEITLMMRFADCVPVMFYDPKKQVVGIAHAGWQGTVKKIASATVLAMQKHYGCLPAD
                     IWAGIGPSIGPDHYEVGTDVIEQVRQSFGARSEEILISVNGHTHLNLWLANKIALNES
                     DVNTVSIAEECTACHLEDWYSHRGEHGKAGRFGAVLALED"
BASE COUNT          605 a          717 c          779 g          773 t
ORIGIN      
        1 gcgttgccat ccactctact tactggcata acaattatgg cgaaccaatg tcgcatggct
       61 gcgtgaatgt ccgcccagat gatgcccgat ttgtttatcg atggtcattg ccacaatcgc
      121 catacgacga tggcaaaatt gaggtgtctg gatatgagaa tgtaacccgc gtgatcgtgc
      181 gggaggaata ggcgcatggc tgccgatgtg accatcgggt tagcgtttct ggctggcctg
      241 gcatctttcc tttctccctg cgtccttgcg cttgttccgg cctatgtcag ctatctcagc
      301 gggcgcgcca ttcgctccaa tcaggctgac gcacaacctg accgctggct gacgctctgg
      361 catggctttg cttttgtgct gggatttagc gtggtcttta tcttaatcgg attggccgcc
      421 tctgctcttg gcgctctgct gtatgatatc cgtaactggt tggcccggtt gggtggcctc
      481 attgttttat tgtttggcct gcatctgacc ggtctgctgc gcctgccttt tctggaatac
      541 gatctgcgcc cgcaatcccg tctggatcgt cagggcagct ttgtctcatc gtttctgatg
      601 ggcattgtat tctctgcggg ttggtcgccg tgcactggcc caattctggg tgcgattgcg
      661 actctggcaa tgaatactgc caacctcccg ctgggcgcct tattattgac agcctattcc
      721 ctaggcctgg ctgtcccatt tttagctgcg gcgctaagta tcggctgggt cacaactgca
      781 ctgcgtaggt atcaaaaact gcttcacttt attgaaatcg caatggggat tatattgatt
      841 gtagtgggcg tgatgatgtt gttgggtatt tttcaacaat tggcattgct ggcgcccggc
      901 gttgattttg gtttatgaga ggcattaatg accattactg caacattata ttacaaacct
      961 ggtgacgaag atcgcacctt tgaaatcaag caaactcttg cggatttaca ggccagtatg
     1021 ccgcacgaac ttatattgat ggatggaacg aagtaccctg cggttggtgt tgaacccgat
     1081 acccagttgc cggttttaca aattggccct tatcaactga aaggccagtt cacgcctcag
     1141 gaggttgagg ttgcactcag ggcggcccaa gatcgagaat cggcgctcca acggattgat
     1201 ggcagcgccc ttgcgtctac tggcatcacc gtgacgaacg gtgatcgcct gagcctctgg
     1261 ttaagcaagc attatatgtg ggtcattatt ctgtccctgg ccgtgtatgc aggtctgccg
     1321 ttcctggccc ccgtgttaat gaaaactggc gctgctgctc ctgcccgaat aatttacaaa
     1381 atctacacac ctctgtgcca ccaattagcc ttccgctcct ggtttttatt tggggaacag
     1441 agcttctacc ccagcgaact ggctgaaatg gataacgtca tttcatacga agcctggatt
     1501 ggcaaggaag gcatccgctt atcagaggac ccgtccactt caaacgcgtt tattattcaa
     1561 gcgcgggata cgctgggcga tgaacgtatg gggtataaag tggctctgtg ccagcgcgat
     1621 gtggcgattt atggcagtat gatgttattc ggcattctgt ttgcaataac cggccgtcgc
     1681 tggaaagggc tttcctggaa gtggtggttg attattggca tcgtcccgat agccttggat
     1741 ggcggatccc agctaccagg cctgctgagc tggctgcctt tctccttgcc gattcgtgag
     1801 agcacaccct ttttgcgcac tttgaccggg acactgtttg gcataatgac cgcctggtat
     1861 ttattcccgg ttattgaaga aagcatgcgc gaaacccgcc agatggtgga ctggaaatat
     1921 gcgcaagccc ggcgtactcg cgaggttgtt tcctgatggc ctggcgggat tacggcgaac
     1981 tgcgtgcata cgaatttgag accctcccgg tcttacatgg ctttttcacg cgacgcgggg
     2041 gcgtcagccc ggcgccctgg gaatcgctca atcatggcgg cactgttggt gatattcgcg
     2101 ccaacgtggt cgaaaaccgg cggcgggttt ttgcggcaat tggtcgtccc gtagaatcta
     2161 tatttgatgt gtggcaggtc catagtgcaa cagtggttgt cacagatgcc ccgcgcccat
     2221 tggatcaacc acatcaatca gcagacgcaa tcattaccaa taatccagaa atcactctga
     2281 tgatgcgctt tgcagattgt gtgccggtga tgttttatga tccaaagaaa caggttgtcg
     2341 gtattgccca tgctggctgg cagggcactg tcaagaaaat tgcatctgcc accgtgttgg
     2401 ccatgcaaaa gcattatggc tgtcttccgg cagacatctg ggctggcatt ggcccttcaa
     2461 ttggaccaga tcactacgaa gtaggtacgg atgtgatcga gcaagtccgc cagtcttttg
     2521 gggcgcgttc agaagagatc ttgatctcag ttaatggcca tacgcattta aacctctggc
     2581 tggcaaacaa gatagccctg aatgaatccg acgttaatac tgtgagtatt gccgaggagt
     2641 gtaccgcctg ccatttggaa gattggtatt ctcaccgcgg cgagcacggt aaagccgggc
     2701 gttttggagc tgtattggca ttggaggatt gatcagatga ctgcattggt tgatcaaatt
     2761 cgcgaaaata ttagtcgagt gcaaacccgt atcgataagg ccgaaacccg ctccggacgc
     2821 cagccgggtt cggtaaaact ggttgtcgta accaaagcac aacccattac cgtt
//
LOCUS       DAOPVS010000208         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_36204_length_2863_cov_51.858618, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000208 DAOPVS010000000
VERSION     DAOPVS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36204_length_2863_cov_51.858618"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PKL11_10675"
     CDS             complement(<1..1200)
                     /locus_tag="PKL11_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14105.1"
                     /translation="MQTPNRIVRLHPGEFPLVLILGLVLLVNSLTMQLGGIVAVSGFL
                     NTGGINSILIVYLVDYALIFLSGVFQSLIVDRFDRTKLVKAMALLFALVYLVLRLLFW
                     LGAPDWLNYAITFVFAEQQFVFFPLVFWVLANDLYSMEQSKRLFPLISSLSFVGELIG
                     IGLAIAAPVIMLALNVSIEEVLTLNAILYLGAWLSLHFGLRGLKPRTTVRQKQNLRET
                     LVEGWDFVCNVDSFRYLMYVIIALAASWLMLEFRFLGITDQTYPDQFSYQRFFSIFRL
                     VLALGGFAMQALFTSRLLAAMDLRRAFFILPAAALIGAVLMLVFPGATPVIAGIGIYM
                     LARLTIHDSAIKSLESLIPEERRGRASTFLDGYLPAVGVIGGVLLTGVVVLIGQWTQT
                     DLHWLYLV"
     gene            complement(1213..1971)
                     /locus_tag="PKL11_10680"
     CDS             complement(1213..1971)
                     /locus_tag="PKL11_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNZ14106.1"
                     /translation="MGKIVSVHSFRGGTGKSNSTANLAAQLALLGKCVGIVDTDIQSP
                     GIHVLFNLQEEAMVHSLNDYLWGKCEIQDAAMDVTSNLGVPISGKIWLIPSSIKTGEI
                     VRVLREGYDVSLLNDGFQKLVEELNLDVLLIDTHPGLNEETLLSIAISDVLIIIMRPD
                     QQDYQGTAVTLDIARKLGVARIALLVNKIPQVFDLADVRARVERIYQIEVAGLLPHSD
                     DMMALASAGVFSIKFPDHPISKIYRQVVTQQILG"
     gene            complement(1990..2388)
                     /locus_tag="PKL11_10685"
     CDS             complement(1990..2388)
                     /locus_tag="PKL11_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14107.1"
                     /translation="MGGVFDRLQQRLSVQKREEGITALELAELPPNLRKLMRLMLREI
                     EMDYPAICAAIEAMPESGRLKKDELDQALAELTKQNWLQRTVRSGIATYQVNLRRKAG
                     VTLPPGIWTALDERIAQSKQTPTEPETKES"
     gene            complement(2410..2538)
                     /locus_tag="PKL11_10690"
     CDS             complement(2410..2538)
                     /locus_tag="PKL11_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14108.1"
                     /translation="MPSFLAMFLGGDNTPKGLAVANVIWCGECEPQPEPIPMPELF"
     gene            complement(2647..>2863)
                     /locus_tag="PKL11_10695"
     CDS             complement(2647..>2863)
                     /locus_tag="PKL11_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14109.1"
                     /translation="MMLVVNKLLEKYPPEETRAYVEQTYGVPVAAVLPLSEDMADLGS
                     ADLFSLCHPEHRWSRGIQQVADKLLAL"
BASE COUNT          703 a          767 c          810 g          583 t
ORIGIN      
        1 caccagatag agccagtgca ggtctgtctg cgtccattga ccaatcaaca ccactactcc
       61 ggtcagcagc acaccgccaa tcacacccac tgccggcagg tagccatcta aaaatgtgct
      121 ggcacggccg cggcgttctt cggggatcag cgactcgagc gacttgatgg ctgaatcgtg
      181 gatggtgagg cgggccagca tgtagatgcc aatgccggca atgacaggtg tggcgccggg
      241 gaagaccagc atcaacacag cgccaatcaa tgccgctgcc ggcaggataa agaaggcgcg
      301 gcgtaaatcc atggctgcca gcagccggct ggtgaagagg gcctgcatgg caaagccgcc
      361 cagggctaac acaagccgga agatgctgaa gaaacgctga tagctgaact ggtccgggta
      421 ggtttggtcg gtaatcccga ggaagcggaa ttccagcatc agccagctgg cggccagggc
      481 gatgatgaca tacatcaagt agcggaagga gtcgacattg cagacaaagt cccagccttc
      541 caccagggtc tcgcgcaggt tttgtttttg ccggacggtg gtacggggtt tgaggccccg
      601 caagccaaag tgcagcgaca accaggcacc caggtaaagg atggcattga gggttagcac
      661 ctcttcgatg gagacattca gcgccagcat gataactggg gcagcaatgg ccaggccaat
      721 gccaatcagt tcgcccacaa agctgagact ggagatgagt gggaacaggc gtttggactg
      781 ctccatgctg tacaggtcat ttgccagcac ccagaaaacc agcgggaaaa agacaaattg
      841 ctgctcagca aacacaaagg tgatggcata gttcaaccag tcgggagcgc ccagccagaa
      901 gagcagccgc aagaccagat agaccagcgc aaagagcagc gccatggctt tgaccagttt
      961 ggtacggtca aaccgatcta caatgaggga ttgaaaaacg ccagacagga agatgagcgc
     1021 ataatccacc agatagacaa tcaaaatgga attgatgccg ccggtgttca agaaaccact
     1081 gaccgccaca atgcccccaa gctgcatggt caacgaattg accagcagca ccagccccag
     1141 gatcagcacc aggggaaact cgcctggatg gagacgaacg atgcggttag gggtctgcat
     1201 agatgctcct gactaaccta agatttgttg ggtcacaact tgtcgataaa tcttgctaat
     1261 gggatggtcg gggaatttga tgctgaagac gcccgcagag gccaaagcca tcatatcatc
     1321 cgaatggggc agcaaaccag ccacttcaat ctggtaaatg cgttcaaccc gggcccggac
     1381 atctgccagg tcgaaaacct gagggatctt attgaccaac agggcgatgc gcgccacgcc
     1441 cagcttgcgg gcaatatcca gcgttacggc agtgccctga taatcctgtt gatcggggcg
     1501 catgatgatg atcaggacat cggagatggc aatggagagc agggtttctt cgttgaggcc
     1561 cggatgggtg tcgatcaaca gcacatccag attcaattcc tctacaagct tctgaaagcc
     1621 atcattgagc aggctgacgt cgtagccttc gcgcagcacc cggacgatct caccggtttt
     1681 gatgctggat gggataagcc aaatttttcc agaaatcggg acgcccaggt ttgacgtcac
     1741 atccatggcg gcatcctgga tctcgcactt gccccagaga tagtcattca aggaatgcac
     1801 catggcttct tcctgcagat tgaagagcac atggatgcct ggcgactgga tatcggtatc
     1861 cacaatgcca acacatttgc ccagcaacgc aagttgggca gccagattag cggtagagtt
     1921 tgatttgcct gtgccgccgc ggaatgaatg tacggatacg attttgccca taagattttc
     1981 ctccaatctt tatgattctt tggtttcggg ttcggtcggg gtttgtttgc tctgggcaat
     2041 gcgttcatcc agagccgtcc agatgccagg aggcagggtc accccggctt tgcgccgcag
     2101 gttgacctga taggtggcaa tgccactgcg aacggtgcgc tgcagccagt tttgtttggt
     2161 cagctcagcc agagcctgat ccagttcgtc tttcttcaag cgcccagatt ctggcatggc
     2221 ttcaatggcg gcgcaaatgg ctggataatc catctcgatt tcacgcagca tcagccgcat
     2281 cagtttgcgc aggtttggcg gcagttcagc cagttccagc gcggtaatgc cttcctcgcg
     2341 cttctgaacg ctcaaacgct gctgtaagcg atcaaatact ccgcccatga tacgtacctc
     2401 gcttcctggt tagaacaact ctggcatggg gatgggctcc ggttggggct cacattcgcc
     2461 acaccagatg acattggcaa cggccagtcc cttgggcgtg ttatcgccgc ccaggaacat
     2521 cgccagaaaa gacggcataa atttggcgtg atctttacat acaccgcggc cgcaaaaata
     2581 gcaggtggct cgcgccggtt catcacaatg ccagcataac atagggggtt cctctaattt
     2641 ccgggctcaa agcgccagta atttatcggc tacttgctgg ataccccgcg accacctgtg
     2701 ttcaggatgg cagagggaga agaggtcggc gctgcccagg tcggccatgt cttcgctcag
     2761 tggcagaaca gcggccacag gaacgccgta ggtctgttcg acatatgcgc gggtttcttc
     2821 gggggggtat ttttccagca gtttattaac caccagcatc ata
//
LOCUS       DAOPVS010000209         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_36519_length_2844_cov_77.999283, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000209 DAOPVS010000000
VERSION     DAOPVS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36519_length_2844_cov_77.999283"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..565
                     /locus_tag="PKL11_10700"
     CDS             257..565
                     /locus_tag="PKL11_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ14110.1"
                     /translation="MKLQEFRRQLEENPEYLKAAEELHLQLLLADAVLKGRLSKGWSQ
                     TQLAEAVGTKQANISRIEAGLANPTLSLIRKILQALDLDLKIQPAAPKPEYTSNSVKQ
                     "
     gene            complement(852..1043)
                     /locus_tag="PKL11_10705"
     CDS             complement(852..1043)
                     /locus_tag="PKL11_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14111.1"
                     /translation="MIYKHNFTLPEELAADIADQGFEFMPELLRLVINAAMQMERQKY
                     LGVEHLRVSIDSHSSLSVH"
     gene            complement(1266..1502)
                     /locus_tag="PKL11_10710"
     CDS             complement(1266..1502)
                     /locus_tag="PKL11_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14112.1"
                     /translation="MKRTGRPAQVIPPIEVHVHLRLRPGEDDDLIAFFRNPEMRCMAR
                     AVKAALRMGGMPSSPAETPMVDDGAGDALADLLL"
     gene            complement(1499..2488)
                     /locus_tag="PKL11_10715"
     CDS             complement(1499..2488)
                     /locus_tag="PKL11_10715"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HNZ14113.1"
                     /translation="MTEALFFGEDLGAGANKLYGPNGGLQLQAIVAADTGQPVTQLLG
                     FRSHKPPLKIGIGGMNFYVGPGAHNWGRGIENLDYERMTGVPETRAIVYGILTRYAQQ
                     YGPLEAPLHLIVGLPLEPLSGEQAQANADAVRRWLIGEHNWEADGQPCSIQVDEVKVT
                     SQPSGALFDYLLDDTGQFIPTRRAHFNQEVGIVSVGFNTLELLTVSDRTVVQGMTAGR
                     TLGVRRLLELLNGENLHTLGELDTLLRAGRLDVRQALPVWAREITGQIEKTWGNRWKR
                     FAQIIIVGGGALLLRDALIDRFNGRLHIPDDPVMAISRGLYKLARQQANRRKA"
     gene            complement(2481..2687)
                     /locus_tag="PKL11_10720"
     CDS             complement(2481..2687)
                     /locus_tag="PKL11_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14114.1"
                     /translation="MTTQETREDGTLVLLVHLTLKPGHDDALIQLVRSAPRRLLARRV
                     REAMHKGLTQPIKSNSEGKAEHHD"
BASE COUNT          659 a          827 c          705 g          653 t
ORIGIN      
        1 ggtcgcctga tggttatacc agacctacca ccctttcggg tagaagttac agaaagaatt
       61 ttacgctaac ttgcaaaatt gaccgaacat cctgattgta tatttcttca ttggtcatta
      121 cccgcgtagc cgcatctaaa gaataaaacg ccacctgtca aaaccggttt cccccattcg
      181 agcaggatta ggttttgtca gcaatcccat caacgcccac agtaagcagt atataattag
      241 aaagcatgag atcaaaatga aacttcaaga gttccgccgg caattagaag aaaaccccga
      301 gtatctcaaa gctgccgaag aactgcatct gcaattactg ctggctgatg ctgtcctgaa
      361 agggcgatta agcaaaggct ggtcacaaac ccagcttgcc gaagccgtgg gcaccaaaca
      421 ggccaacatc tcccgaattg aagccggcct ggcaaaccca acgctttcac tcatccgtaa
      481 aatcttgcag gcgctcgacc tggatctgaa aatccagcct gccgccccca aacccgaata
      541 cacctcaaac tcagtcaaac aataacaaat cataacgcac aaacctttag catccggaaa
      601 taatcgtcat ttataggccg ccctactttt agcagagtag ggcggcctac atttgggtat
      661 ctcagaaagc ataggacgcc ctataaccca cgtttcgcac ctgtgggtag gcatttttag
      721 gaatttcttg taagcttggg ggatgagcgc tgaaccaaaa tatgaaaccc aacggataga
      781 ccaccttggg atagtggctg gcgtctgcca ggaaatatcc ctgatcgaac agatcgacag
      841 gcaagtcaag gctagtgaac ggaaagtgag ctgtggctgt caatagacac acgtaaatgc
      901 tcgaccccta gatatttctg ccgttccatt tgcatggccg cattgatcac caggcgcagt
      961 aattccggca taaactcgaa tccctggtca gcaatatctg ccgccaattc ctctggtagg
     1021 gtaaaattat gtttgtagat catggttcct cctttgtggt ttggtcacct taaggataac
     1081 ctgatctaca ctttctaagt ggagttcctc ttcagtttcc ctgcgtccct ctcgtagatt
     1141 tgcctttcat attgctcgct ccaaaaaatt ggcatgtttt cctcgctctt cttgtaagta
     1201 cccaaaaact ggatttttta gctaagggaa ccagaaaccg ggcgggagtt tctggttccc
     1261 aatcattaca gcagtagatc cgccagcgca tcgccagcgc catcatccac catgggtgtt
     1321 tcagcagggg atgatggcat tccgcccatc cgcagggctg ctttgaccgc ccgcgccatg
     1381 cagcgcatct ctggattgcg aaaaaacgca atcaggtcgt cgtcctcgcc gggccgcagg
     1441 cgcaggtgaa catgcacctc gatgggcggg ataacttgcg ccgggcgccc ggtccgcttc
     1501 atgctttcct ccggttggct tgctgccggg ccagtttgta cagcccgcgg ctgatcgcca
     1561 tcaccggatc gtccgggata tgcagccgcc cgttgaagcg gtcaatcaat gcatctcgca
     1621 gcagcagcgc tccaccgccc acgatgatga tttgtgcaaa ccgcttccac cggttgcccc
     1681 aggttttttc gatttgcccg gtgatctcgc gtgcccagac cggcagcgcc tggcggacat
     1741 ccagccgccc agcccgcagc agggtatcca gctcgcccag ggtgtgcaga ttctcgccgt
     1801 tgagcagttc cagcagccgc cgcacaccca gcgttcggcc agccgtcata ccctgcacca
     1861 ccgtccggtc actgacggtc agcagttcca gtgtgttgaa acccaccgag acgatcccca
     1921 cttcttgatt gaagtgcgcc cgccgggttg gaatgaactg cccggtatca tccagcaggt
     1981 aatcaaacaa agcccccgac ggttgcgagg tgactttgac ttcatccacc tggatagagc
     2041 agggctgtcc atcggcctcc cagttgtgtt cgccaatcag ccagcggcgc actgcgtccg
     2101 cgttggcctg cgcctgttca cccgagaggg gctccagcgg caaacccacg atcagatgca
     2161 gcggagcttc gagcggccca tactgctggg catagcgggt cagaatgcca tagacaatgg
     2221 cgcgggtttc cggcacaccg gtcatccgct cgtaatcgag attttcaatc ccgcgacccc
     2281 agttgtgcgc gccagggcct acgtagaaat tcatcccgcc aatcccaatt ttgagcggcg
     2341 gcttgtggct gcgaaaaccg agcagctgcg tcaccggctg cccagtatcc gccgcgacaa
     2401 tggcctggag ttgaaggccg ccgtttggcc cgtataattt gttggcgcct gcgccaagat
     2461 cttcaccgaa aaacaaagct tcagtcatga tgttctgcct ttccttctga gtttgatttg
     2521 atgggttgtg tcagtccctt gtgcatcgct tcacgcactc gcctggccag cagccgccgt
     2581 ggcgcgctgc ggaccaattg aatgagcgcg tcgtcatgcc caggtttgag tgtgagatgg
     2641 acaagcaaga ctaatgtgcc gtcctcgcgc gtttcttgtg ttgtcattgc gacctcctga
     2701 aaaagttggc cgcacccaaa cgaaaggggg aaccccactc gactgccact gtgtggacga
     2761 ttcgtgcaat attcgtgcaa taggcatgga atctatgtaa aatatgcact ctgtggactg
     2821 ttttatcccc catatatact atct
//
LOCUS       DAOPVS010000210         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_36607_length_2839_cov_54.534842, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000210 DAOPVS010000000
VERSION     DAOPVS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36607_length_2839_cov_54.534842"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..587)
                     /locus_tag="PKL11_10725"
     CDS             complement(84..587)
                     /locus_tag="PKL11_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNZ14115.1"
                     /translation="MNGSVLVLNANYEPINICSLQRALGLILMEKASLVANGRGVVHT
                     PGRAYPCPSVIRLDYMIHRPRPHLKLTRQEVFRRDHETCQYCGKHTHNLTIDHIVPRH
                     LGGRHIWTNVAAACPACNHRKGGRMLTEAGMRLLHVPQEPPSSVSYIFGRYLPENTEW
                     EPYLSGW"
     gene            701..>1154
                     /locus_tag="PKL11_10730"
     CDS             701..>1154
                     /locus_tag="PKL11_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ14116.1"
                     /translation="MKVIITGGTGLIGRALGQSLVARGHQVWALTRSPQSAHLPSGIE
                     PVGWDGETPHGWLPLVEQADAVINLAGENIGAGRWSLERKARILNSRLNAGRAVVQAI
                     QAASHRPHILIQASAVGYYGPSDNTLLTESSPAGQDYLAQVAVAWEKST"
     assembly_gap    1155..1254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1255..1479
                     /locus_tag="PKL11_10735"
     CDS             <1255..1479
                     /locus_tag="PKL11_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HNZ14117.1"
                     /translation="PQPLSQSDFGRVLGRVLRRPYWLPAPAFALRLALGEMSTLVLDG
                     QRAVPSRLLELGYRFAYPDLEPALNQILKT"
     gene            1539..2750
                     /gene="tyrS"
                     /locus_tag="PKL11_10740"
     CDS             1539..2750
                     /gene="tyrS"
                     /locus_tag="PKL11_10740"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNZ14118.1"
                     /translation="MNIEEQVAYLMQGTEYGDESLRRTMEQELRERLIEAQKEGRPLR
                     VYCGYDPRKADLHLGHTVTMRKLRQFQELGHEVTFVVGTYTSLIGDPSDKDVLRPRLV
                     QEEVEENARTYAEQAFRILDRERTIIRYNAEWLAGITFEQLIHLASNFTIQQFLTREA
                     FRNRWDRNDPIYLHEFFYSIMQAYDAYTLKADVQVGGTDQMFNIVTASRKLMTYLGAK
                     PNIGIILAILPGTDGVVKMSKSLGNHIPINTDAADMYGKMMSIPDMAMPSFARLVTRW
                     TPEQIKAWETGVQSGSLHPRDAKMQLAQEVTASFYGEAEAEQAQQVFVRMFQQREVPT
                     EMGTFALQPGQSVVDVIVAAGLAASKSEARRLVQQKGVRLDGAVLEDPLAPFPHAGVL
                     QVGRRHYLRIV"
BASE COUNT          588 a          840 c          717 g          594 t
ORIGIN      
        1 atccatctcg cgaccgatct gatggaaatc acgctggttt agtgctcctt gccattaagt
       61 aaaatggcaa acatattgaa ccgttaccac ccggataaat aaggctccca ctcagtattt
      121 tcaggcaggt agcgcccaaa aatataactg actgacgaag gcggttcttg cggaacatgc
      181 agcagccgca tcccagcctc ggtcagcatc cgcccgccct tgcgatgatt gcaggccggg
      241 caggccgcag ccacattggt ccaaatatgc cggccgccca ggtgccgcgg cacaatatga
      301 tcaatcgtca agttgtgggt atgcttgccg cagtattggc aggtttcatg gtcgcgccgg
      361 aacacctcct ggcgggtcag tttgaggtgc ggtcgcggtc tgtgaatcat gtaatccaat
      421 cgtatcaccg aagggcaggg gtaagcccgc cctggcgtgt ggaccacacc gcgcccattg
      481 gccaccaggc tggccttttc cattaagatt aaccccaggg cgcgctgcag cgagcagatg
      541 ttgatcggct cataattggc gttgagtacc agcacactcc cgttcatcgt gaataccctc
      601 tgcacattat agcacgcctc tgtataatga tttctacgga atagacaaac gcttcaggtt
      661 caaatcccgt ataattcaat tatcaatact ggaggcgatt atgaaagtca tcatcaccgg
      721 cggcacaggt ttaatcggtc gggctctggg gcaaagtctg gttgcccgcg ggcatcaggt
      781 ttgggcgctg acccgttccc ctcaatctgc ccatttacct tctggtatag aacctgttgg
      841 ctgggatggt gagacgccgc atggctggct gccgcttgta gagcaagccg atgcggtaat
      901 taacctcgcc ggtgaaaaca tcggcgccgg gcgctggtcg ctggagcgca aagcccgcat
      961 cctcaacagc cgcctgaatg cgggtcgggc tgttgttcag gcgattcagg cggctagcca
     1021 ccgccctcat atactgattc aggcctcagc agtcggctat tatggcccca gcgacaatac
     1081 cttactaacc gaatcctcgc ccgccgggca ggattatttg gcccaggttg cggttgcctg
     1141 ggaaaaatca accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccag
     1261 ccgctgtctc agtctgattt tggccgggtg ctggggcgcg tcttgcggcg accctactgg
     1321 ctgcctgctc ccgcctttgc cctgcgcctt gccctcggtg agatgagcac cctcgtcctc
     1381 gatggtcagc gtgctgttcc atcgcgcctg ctcgaactcg gctaccgctt tgcctatccc
     1441 gatctcgaac ccgccctcaa ccagatcctg aaaacctgaa aatccccgtt ccccaaaaca
     1501 gcaatcctga tgatataatc gccgttcagg aggctgttat gaacattgaa gaacaagtcg
     1561 catacttaat gcagggcacc gaatatggtg acgaatccct gcgccgcacg atggagcagg
     1621 agctgcgtga gcgtttgatt gaagctcaga aagaaggccg cccattgcgc gtttattgcg
     1681 gctatgatcc tcgcaaagcc gacctccatc tgggccacac cgtcaccatg cgcaagctcc
     1741 gccagttcca ggaactgggc cacgaagtga cctttgtggt tggcacctac acttctttga
     1801 tcggcgatcc ttccgacaaa gacgttttgc gcccgcgcct cgttcaggaa gaagtggaag
     1861 aaaacgctcg cacctatgct gagcaggctt tccgcatcct ggatcgtgag cgcaccatca
     1921 ttcgttacaa cgccgaatgg ctggctggca ttaccttcga acaactcatt catctggcat
     1981 ctaatttcac cattcagcag ttcctcaccc gcgaggcctt ccgcaaccgc tgggatcgca
     2041 acgaccccat ctacctgcat gagttcttct actccattat gcaggcttat gatgcgtaca
     2101 ccctcaaggc cgatgtgcag gtgggcggca ccgatcagat gttcaacatc gtcaccgcct
     2161 cacgcaagct gatgacgtat ctcggcgcca agcccaacat cggcatcatt ctggcaattt
     2221 tgcccggcac cgatggcgtg gtgaagatga gtaaatccct cggcaaccac atccccatca
     2281 atacggatgc tgccgatatg tacggcaaaa tgatgagcat ccccgatatg gccatgccat
     2341 cctttgcccg cctggtcacc cgctggaccc ccgaacaaat caaagcctgg gagaccggtg
     2401 tgcaatctgg cagcctgcac ccccgcgatg ctaaaatgca gcttgctcag gaagtgaccg
     2461 cgtcattcta cggcgaagcc gaagctgagc aggctcagca ggtctttgtg cgtatgttcc
     2521 agcagcgtga agtccccacc gaaatgggta cttttgctct acagcccggc caatccgtcg
     2581 ttgatgtgat tgtcgctgcc ggtttggccg ccagcaagag cgaagcccgc cgcctggtac
     2641 agcagaaagg cgtccgcctg gacggtgcag tcctggaaga tccgctggct cccttcccac
     2701 acgctggcgt tctgcaagtt ggccggcgcc actacctgcg catcgtctga tcgcaccgcc
     2761 gcagggcatc cataaataaa tccgcatacg gcagcccggg tgaagcatct tcccgggctg
     2821 cttcaatgca ataccgcac
//
LOCUS       DAOPVS010000211         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_36892_length_2824_cov_32.346334, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000211 DAOPVS010000000
VERSION     DAOPVS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36892_length_2824_cov_32.346334"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PKL11_10745"
     CDS             <1..1542
                     /locus_tag="PKL11_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNZ14119.1"
                     /translation="IQRVMGQLTAHMKGQTPHFECEYRALHDNGSYRWVLVRGLATRD
                     EKQQAVRMAGSMSDITARKAIEEQLSHDAMHDTLTGLPNRAYVIEQLQRCMEICRRRP
                     TYLAALLLLDLDRFKVVNDSLGHLCGDELLVSVGERIRDCLRPGDVVGRFGGDEFAVL
                     LEDLHNPGEVAVVARRLLEALEKPFKIEGREVFTSASIGITMGNGDYERPEDIIRDAD
                     TAMNRAKAKGRARYQIYDVEMHASSISQLELETDLRRAIERQELRVYYQPIISLQTRR
                     ITSLEALVRWQHPRRGLTLPGEFMPLAEDTGLVLPIGEWVLRTALTDLKVWREVGFKD
                     LQVAVNVSVRQLESQDLIGLVKSALKDTGLPAQALQLEITESAAMHDVEQTAKILDVL
                     GKMGIDISIDDFGTSYSSLGYLNRFPVRHLKIAQTFTRNIPDRRDDTAITHAIIALAH
                     ALKLTVIAEGVENERQLATLIPLGCEQIQGYMISRPQPADAIEQVLNESPKNLKIREL
                     PSRLN"
     gene            complement(1539..>2824)
                     /locus_tag="PKL11_10750"
     CDS             complement(1539..>2824)
                     /locus_tag="PKL11_10750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNZ14120.1"
                     /translation="VMNTPVMRQAAESGRPVIVADAAAVIRQQSSEHMQWVRSLATIP
                     IQHQGVTIGFINLQSATPNAFSANQSDTLQAYANQAALALQNARLFETVRENAHRLSI
                     LNEIGRIAISAPSLEALLDGLANRVQSLFGMDAAYITLWDEANHRPIPASASGQQGAA
                     YSATEVVNGEVTLTESVLNARRILIVEDAYHSSYISSRIARHFNEASIMGIPLIVGDQ
                     KLGALIMGYTQPHKFTPDEVSLAEQAGRQIALAVAKAELVAEVQRLAVTDDLMGLYNY
                     RGLMDLGAQEVDRAQRYGRPLSAIMMDIDRFKTLNDTYSHIVGNEILCQLAERCRQVV
                     RDADIVTRYGGDELCILLVETDDVTALHVAERIRQSVAGAPFLVSVGAIPATVSLGVS
                     ALKPGETLNQLIARADRGLYHAKEGGRNRAVFEAD"
BASE COUNT          645 a          703 c          888 g          588 t
ORIGIN      
        1 attcaacgcg tgatggggca actgaccgcc catatgaagg gccaaacacc gcacttcgag
       61 tgcgagtatc gggcactgca cgacaacggt tcataccgct gggtgctggt gcgcgggctg
      121 gcaacccggg atgagaagca gcaggctgtg cgcatggctg gctcgatgag tgatattacg
      181 gcacgcaaag ccattgagga gcagttgagc cacgacgcga tgcacgatac gctgaccggg
      241 ctacccaacc gggcttatgt gattgagcaa ctgcaacgct gcatggaaat ttgccgccgc
      301 cgccccactt acctggcagc attattgctg ctggacctgg accgattcaa agttgtgaac
      361 gacagtctgg ggcatttgtg cggcgacgaa ctactggtat cggttggcga gcggattcgg
      421 gattgcctgc gccccggcga tgtggttggg cgattcggcg gcgatgagtt tgcggtgctg
      481 ctggaagatt tgcataaccc aggcgaagtg gccgtggtgg cccggcggct gctggaagct
      541 cttgagaagc cattcaagat tgaagggcgg gaggtgttca cctcggcctc aattgggatc
      601 acaatgggca atggcgatta tgaacgcccg gaagatatta ttcgggatgc ggatacggcg
      661 atgaaccggg ccaaggcaaa gggccgggcg cgctatcaga tttatgatgt agagatgcat
      721 gcatcatcca tctcgcagct tgaactggaa accgatttgc ggcgggcaat tgagcggcag
      781 gaactgcggg tatactacca gccgattata tcgctgcaaa cgcggcggat cacctcgctg
      841 gaggcgctgg tgcgctggca gcatccccgc cgcgggttga cgctgcccgg tgaatttatg
      901 cctctggctg aagatacagg gctggtgctg ccgattggag aatgggtttt aaggactgct
      961 ctgactgatt tgaaggtctg gcgtgaggta gggtttaagg acctgcaggt tgccgtgaat
     1021 gtatctgtgc ggcagttgga gagccaggat ttaattgggc tggtaaaatc ggcgcttaaa
     1081 gatactggat taccggcaca agcactgcaa ttggagatta ccgagagcgc ggcaatgcat
     1141 gacgtggaac agacggccaa gatcctggat gtgctgggga aaatgggaat tgatatttca
     1201 attgatgatt ttggaaccag ctactcatcg ctgggttacc tgaaccgctt cccggtcaga
     1261 cacttgaaga ttgcccaaac ctttacccgg aatattccgg accggcgcga tgacacggcg
     1321 attacccatg caatcattgc gctggcgcat gcgttgaagc tgaccgtgat tgctgaaggg
     1381 gttgaaaatg agcggcaact ggcaacgctg attccgctgg gatgcgagca aattcaaggt
     1441 tatatgatca gccggccaca gccagccgat gcgattgagc aagttctgaa tgagagcccc
     1501 aaaaatttaa agatccggga actgccatcc aggctgaatt aatcggcttc aaagacggcc
     1561 cggttgcgcc cgccctcttt ggcatgatag aggccacggt cggcgcgggc gatgagctga
     1621 ttgagggttt cgcctggttt gagggcggaa acccccaggc tgaccgttgc cggaatggcg
     1681 cctacactga ccaggaacgg cgccccggcg accgactggc ggatgcgctc ggcaacatgc
     1741 aaagcggtca cgtcatcggt ttcaaccaga agaatgcaaa gctcatcacc gccatagcgg
     1801 gtgacgatat cggcatcacg cactacctga cggcagcgtt cggcaagctg gcataagatt
     1861 tcgttgccga cgatatgaga ataggtgtcg ttcagggtct taaagcggtc aatatccatc
     1921 atgatggctg ataaaggacg accgtaacgc tgggcgcggt caacctcctg ggcgccgaga
     1981 tccatcaggc cgcggtagtt gtacagaccc atcaaatcat cggtaacggc caggcgctga
     2041 acctcggcga ccagctcggc cttggccacg gccagagcaa tctggcgccc ggcctgttca
     2101 gcaagagaga cctcgtcggg ggtaaatttg tggggctggg tatagcccat aatcaaagcg
     2161 cctaattttt gatcaccaac aatcaggggg attcccatga ttgaagcttc attaaaatgg
     2221 cgggcaatcc gagagctgat atacgatgag tggtaggcgt cttccacaat caggatccgc
     2281 cgggcgttaa gcaccgattc ggtcagcgta acttcgccgt tgaccacttc tgttgctgaa
     2341 taggcggccc cctgctgacc agaggcagaa gcggggatgg ggcggtgatt ggcttcatcc
     2401 cacaatgtga tgtaggcggc atccatacca aagagggatt gaacccggtt agccagaccg
     2461 tctaataaag cttccagcga aggcgccgat atggctatac gacctatttc atttagaatg
     2521 gacaggcggt gggcattttc tcggacggtc tcgaacaggc gcgcgttttg caaggccagg
     2581 gccgcctggt tggcgtaggc ttgaagcgta tcggactgat tggcagaaaa cgcattggga
     2641 gtggcacttt gaagattgat gaagccaatg gtgacgcctt ggtgttgaat tgggatggtg
     2701 gccagcgagc gcacccactg catatgctca gagctttgct ggcggataac agctgcggca
     2761 tcggccacga tgaccgggcg gcccgattcg gcagcctggc gcatgacagg tgtgttcata
     2821 acgg
//
LOCUS       DAOPVS010000212         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_36915_length_2823_cov_40.740968, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000212 DAOPVS010000000
VERSION     DAOPVS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36915_length_2823_cov_40.740968"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="PKL11_10755"
     CDS             <1..1976
                     /locus_tag="PKL11_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNZ14121.1"
                     /translation="SEGYHLMVEAGTGTGKSYAYLVPAALWALQNNARVVISTNTITL
                     QDQLIKKDIPDLKAALNVNLQAAVLKGRSNYLCPRRLAILRQRGPETPEQMRLLAKVL
                     VWLHEGGQGDRAEINLNGSERLVWSSLSAEDEGCKAEVCMSRTGGACPFFISRQAANS
                     AHLLIVNHALLLSDSVTGNRVLPEYNYLIVDEGHHIEDATTGALSYRVTQADMERLLR
                     ELGGSSSGILGRILTQLKAVLRPSDFAGVHQTIDRLTDLGFRAEYDFKQFFQAVFDFL
                     EAMREGQPMGTYSQQVRIIPATRNQPAWMDVEVAWDSTRETLNLLLNLAGQFHKDISG
                     LGEMMPEELEDALGSLATVHHRLTEADTYLSSLASDPDERFIYWAELQPNGGRMALQI
                     APLHIGPLMEQYLWHEKASVILTSATLTTNGEFDYMRSRLNADEADELVVGSPFDYES
                     AALLYLANDIPEPSDPSYQRAVEQAILNLAKATGGRMLALFTSYAQLKRTSQAIAPAL
                     AEQNIEVYEQGEGASANTLLETFRLSERAVLLGTRAFWEGVDIPGEALSVLVIVKLPF
                     DVPSDPIVAARSETFEDPFSEYSIPEAILRFRQGFGRLIRTQSDRGVVAVLDRRLLTK
                     RYGKMFIESLPECAKKVASVRDLPATAARWLNL"
     gene            2091..2543
                     /locus_tag="PKL11_10760"
     CDS             2091..2543
                     /locus_tag="PKL11_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14122.1"
                     /translation="MPSTPAYLQRIEAARSGTNPAVVCRVASGWVFLANMQYLSGYCI
                     LQSDPVVESINALEHEQRTAFLGDMAVVGDAILEVTGAYRINYAIMGNSEPVLHAHIV
                     PRYLSEPEELLHGLPWSYPQAQMDAVGFDPGRDQALMQKLAQAIQKRQ"
     gene            complement(2552..>2823)
                     /locus_tag="PKL11_10765"
     CDS             complement(2552..>2823)
                     /locus_tag="PKL11_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ14123.1"
                     /translation="GRIAIRGEVEEDQIIVQVSDTGLGIPQLDLSYVFDKFYRGSNVT
                     GDVHGVGLGLAIVRSIVENHGGRIWVESTPNQGSIFTVVLPLAQL"
BASE COUNT          611 a          761 c          861 g          590 t
ORIGIN      
        1 tatcagaagg ctatcacctg atggtggagg ctgggaccgg cacgggcaag tcttacgcct
       61 acctggtgcc ggcagcgctc tgggcattgc aaaataatgc ccgggtggtc atttcgacca
      121 ataccatcac actgcaagat cagttaatca agaaagatat tccggatctg aaggccgcac
      181 tgaacgtgaa cctgcaggcc gccgtgttga aaggccgatc caactatctt tgcccgcgcc
      241 ggttggctat tttgcggcag cgcggccccg aaacccccga acagatgcgc ctgctggcaa
      301 aggtgctggt ctggctgcat gagggcggtc agggagaccg ggcagaaatc aacctgaacg
      361 ggtcggagcg tttggtctgg tccagtttgt cggcggaaga tgagggctgc aaagccgaag
      421 tgtgcatgag ccgcaccggt ggagcctgcc cgtttttcat ttccaggcag gcggccaact
      481 cagcgcacct gttgattgtc aaccatgcgc tgctgctgtc ggattcggtg acgggaaacc
      541 gggtgctgcc cgaatacaat tatctgattg tggatgaagg gcatcatata gaagatgcca
      601 ctaccggggc attgagctac cgggtgacgc aggccgatat ggaacggctg ctgcgagagc
      661 tgggcggaag ttccagcggg attttggggc ggattctgac gcaattgaag gcggtgctgc
      721 gcccttctga ttttgcgggg gtgcatcaga ccattgaccg gttgaccgac ctgggcttcc
      781 gggcggagta tgatttcaag cagttcttcc aggcggtttt tgattttctg gaagcgatgc
      841 gcgaggggca gccgatgggc acctacagcc agcaggtgcg catcattccg gcaacccgca
      901 accagccagc gtggatggat gtggaagtgg cctgggattc gacacgcgag acattgaatt
      961 tgctgctcaa cctggccggg caatttcata aagacatcag cgggctgggc gagatgatgc
     1021 ccgaagagtt ggaagatgcg ctgggcagcc tggcaactgt ccatcaccgg ttgacggagg
     1081 cggacactta cctgtcttca ctggcctctg acccggacga gcgtttcatt tactgggcag
     1141 agctgcagcc caacggcggg cggatggcgc tgcaaattgc gccgctgcat attgggcccc
     1201 tgatggagca gtatttgtgg cacgaaaaag ccagcgtgat cctgacctcg gcaacactga
     1261 ccaccaacgg tgagtttgat tatatgcgca gccgcctgaa tgccgatgaa gctgatgaac
     1321 tggtggttgg ttcgccattt gattacgaat cggcggcgct gctttatctg gcaaatgata
     1381 ttcctgagcc atctgacccg tcttaccagc gggcggttga acaggccatt ttgaacctgg
     1441 ctaaggctac cggcgggcgg atgctggcgt tgttcacctc ttacgcgcag ctcaaacgca
     1501 cctcacaggc aattgccccg gcgctggccg aacaaaatat cgaagtctat gagcagggcg
     1561 aaggcgcatc tgccaacacg ctgctggaaa ccttccggct gtctgagcgg gcggtgctgc
     1621 tgggcacgcg ggccttctgg gaaggcgtgg atattcccgg cgaggcgctt tcggtgctgg
     1681 tgattgtcaa actgcctttt gatgtaccct ctgacccgat tgtggcggcg cgctcagaaa
     1741 cttttgaaga tcccttctct gaatacagca tccccgaagc gattttgcgc ttccgtcagg
     1801 gctttgggcg gctaatccgc acccagtctg atcgcggggt ggtggctgtg ctggaccgcc
     1861 gcctgctgac caagcgctac ggcaagatgt tcatcgagtc gctgccggag tgcgccaaaa
     1921 aagttgcttc ggtgcgagac ctgccagcaa cggcggcacg atggctgaac ctgtaaattg
     1981 acttaagtac gcccaaggag ccgtgagatg gcgtcaaaag aaaaagtgca cctgaaccgg
     2041 tttgctgccg ccaatctggc acaccggatt atccgactgg agctgtaata atgccatcca
     2101 cgccagctta tttacaacga attgaagcag cacgtagcgg aaccaatccg gcggtggttt
     2161 gccgggtggc ctcgggctgg gtctttctgg caaacatgca gtacctgagt ggttactgca
     2221 tcctgcaatc tgatccagtg gttgaatcga tcaacgcgct ggagcacgaa caacgcacgg
     2281 catttctcgg cgacatggca gtggtgggcg atgcgatatt agaagtgacc ggcgcctacc
     2341 ggattaacta tgccatcatg ggcaactcgg agccggtgct gcacgcacac attgtccccc
     2401 gctacctgag cgagcctgaa gagctgcttc acggcttgcc atggtcatat ccacaggcac
     2461 agatggacgc ggtggggttc gacccggggc gcgatcaggc attgatgcag aaattggccc
     2521 aggccattca gaaacggcag taaaagtgta actacaactg cgccagcggc agaacaacgg
     2581 tgaagatgct gccctgattg ggcgtggatt cgacccagat gcgcccgccg tgattttcta
     2641 caatagagcg gacaatagcc aagccaaggc ccacgccgtg aacatcaccg gtgacgttgc
     2701 tgccgcggta aaacttgtcg aagacatagc tcaagtccaa ctgcgggata cccaggcctg
     2761 tatcactgac ctgcacaata atttgatctt cttccacttc gccgcggatg gcaatgcggc
     2821 cag
//
LOCUS       DAOPVS010000213         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_37367_length_2797_cov_63.273523, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000213 DAOPVS010000000
VERSION     DAOPVS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37367_length_2797_cov_63.273523"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..629)
                     /locus_tag="PKL11_10770"
     CDS             complement(3..629)
                     /locus_tag="PKL11_10770"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNZ14124.1"
                     /translation="MNLDLIILIIAYLGVGAGAVSGVFEARRKDMDIVGATTVALITA
                     LGGGTLRDMLLGRGPVFWVKEPGYPLVGFAVAILTFYSTRLLKLTARSILIPDALALG
                     VFTVMGAARALEQGTAVFIAALMGMVTGVFGGVLRDVTCNEIPTIFIKNTELYATCSF
                     VGAWVYIGLERAGAQLWVATVASILVTFAFRIISSRFNWRLPEPRSQV"
     gene            complement(687..1094)
                     /locus_tag="PKL11_10775"
     CDS             complement(687..1094)
                     /locus_tag="PKL11_10775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNZ14125.1"
                     /translation="MSACLFCDIVHQRCPARIIYQTETVICFLPRVPEAYGHTLIVPK
                     KHVPDIYAASETLLGELMAAAKKLALHYRTQIQATGVNLLHASGAAAQQSVPHFHLHL
                     LPRFDQDGLNAWPQLPKADFDLDALLQKLQLGE"
     gene            complement(1091..1327)
                     /locus_tag="PKL11_10780"
     CDS             complement(1091..1327)
                     /locus_tag="PKL11_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14126.1"
                     /translation="MVKTIRQVMAVIGVMCIGGILGFVLGALIGGNFATGFRFNGVQG
                     YEATAQIGFFLGALVGLVASGWRSIKRQARWEGR"
     gene            1596..2657
                     /locus_tag="PKL11_10785"
     CDS             1596..2657
                     /locus_tag="PKL11_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14127.1"
                     /translation="MKAKKLLYILVIALFLLTAAACGGDDYEAETETEVEETETIDAE
                     TEETETEEGEAEDTSDDAAASVDTDACNAFLADFFVEDDYSSGDDSEDGEDEGEDEEE
                     DGEDEGEDGEDDDEIILVTYQVNGDEISNPQEESVSDDLIALQQDTETQQEVWDYFIN
                     FMPAEQRERISEFIIFTDGQENVLAAVDQLSVTPDAWSLEIDIADTDDKAELTYTLLH
                     EYGHIMTLNNTQLTTTGGSCTTYQSDEDCTTEGSYLNQFYQAFWTDIYFEWEAAYADD
                     TLDAFYEQYSDQFLTDYAATEPDEDIAESWLYFIISEPPAGDTIAEQKILFFYDFPEL
                     VSLRDEIRSNLCLYLMDEE"
BASE COUNT          646 a          816 c          823 g          512 t
ORIGIN      
        1 gttcacacct gcgagcgcgg ctcgggcagg cgccagttga agcgggaaga aatgatgcga
       61 aaggcaaagg tcaccagaat gctggccacc gtggccaccc agagctgggc gccggcgcgc
      121 tccagcccga tatagaccca ggcgcctaca aacgagcagg tggcatacag ctcggtattt
      181 ttaatgaaga tggtggggat ctcgttgcag gtcacatcgc gcagcacgcc gccaaacacc
      241 cccgtgacca tgcccatcag cgcggcgatg aagaccgcgg tgccctgctc cagcgcccgg
      301 gcggcgccca ttacggtgaa gacgcccagc gccagcgcat cggggatcag gatggagcgg
      361 gcggtcagtt tgagcaggcg ggtggagtag aaggtgagga tggccaccgc aaaccccacc
      421 agcggatacc ccggctcttt gacccaaaag accgggccgc gccccagcag catatcgcgc
      481 agggtgccgc cgcccagcgc ggtgatcagg gcgacggtgg tggcgccgac aatgtccata
      541 tctttgcggc gcgcctcaaa cacgcccgaa accgccccgg cgcccacgcc caggtaggcg
      601 atgatgagga taatcaggtc gagattcatg gacgcgatta tactccgatt gccagacaga
      661 gaaaaatcga ataccccggc gctgttttac tccccgagct gaagcttttg cagcagcgcg
      721 tccagatcaa aatccgcttt tggaagctga ggccaggcgt tcaggccgtc ctggtcaaag
      781 cgcgggagca ggtgcaggtg aaaatgcggc accgattgct gcgccgccgc gccactggcg
      841 tgcagcagat tgacccccgt ggcctggatc tgggttcgat agtgcagcgc caactttttg
      901 gcggcagcca tcagttctcc cagcagtgtc tcagatgccg cgtaaatatc gggcacgtgt
      961 tttttgggca caatcaaggt atgcccgtag gcctcgggca cccggggcaa aaaacagatc
     1021 accgtctcgg tctgataaat aatccgggcg gggcagcgtt ggtggacaat gtcgcagaaa
     1081 aggcaggcgc tcatcgcccc tcccagcggg cctggcgttt gatgctccgc cacccgctgg
     1141 caaccagccc aaccagtgcc cccaggaaaa agccaatctg tgcggtggcc tcgtagccct
     1201 gcaccccatt aaagcggaag ccggtggcaa agttcccgcc gatcagcgcg ccgagcacaa
     1261 aacccagaat accgccgatg cacatgacac cgatgacggc cattacctgt cggattgttt
     1321 tgaccatggg cggggttctc ctctctgatt cctgataatt atgccacaaa agcggatttt
     1381 gttaaccccg agatgccgcc gcgccacggc ttatcttctc tggagtgcgg gtcgcacagc
     1441 acagatgatg ccggctaaag cctgcactcc aagaaagaag ccgctcaatt tgggacgact
     1501 gtccagacaa ttatgctatg attattccat ctctggctct gcccgcagcc cggacaggcc
     1561 cttcccaaaa cctgttttct gataaggata cccccatgaa agccaagaaa ctgctgtaca
     1621 tcctggtgat tgctctgttt ctgctgactg ccgcagcctg cggcggcgat gattatgagg
     1681 ccgagaccga aaccgaggtc gaagagaccg agacgattga cgctgaaacc gaagagaccg
     1741 aaacggagga aggcgaggcg gaagacacct ccgatgatgc cgccgcatcg gtggatacgg
     1801 acgcctgtaa tgcctttttg gcagacttct ttgtggagga tgattattcg agcggcgacg
     1861 actcggaaga tggcgaagac gagggggaag atgaggaaga ggacggtgag gacgagggcg
     1921 aggatggcga ggatgatgac gaaatcatcc tggtcaccta tcaggtcaat ggcgatgaga
     1981 tctcaaatcc gcaggaagaa tcggtgtctg atgatctgat tgccctgcag caggataccg
     2041 aaacgcagca agaggtctgg gattatttca tcaactttat gccggccgaa cagcgggagc
     2101 ggatttcaga gttcatcatc ttcaccgatg ggcaggagaa cgttctggca gccgtggatc
     2161 agctcagcgt gacgccggat gcctggtcgc tggaaattga tattgccgat acagacgaca
     2221 aagccgagct gacctacacc ctgctccacg agtacgggca catcatgacc ctgaacaaca
     2281 cccagctcac caccacgggc ggcagctgca ccacctatca atcagatgaa gactgcacca
     2341 ccgaaggctc ttacctcaac cagttctacc aggccttctg gaccgacatc tattttgaat
     2401 gggaagccgc ctatgccgac gacaccctgg atgcgttcta cgagcagtat tccgaccagt
     2461 tcctgacgga ctatgccgcc accgagccgg acgaagatat tgccgaatcc tggctgtact
     2521 tcatcatctc cgagccgccg gcaggcgata ccattgccga gcagaagatc ctcttcttct
     2581 acgacttccc cgaactggtc agcctgcgag acgagattcg cagtaacctg tgcctgtacc
     2641 tcatggatga ggaatagcca ccgggatatt ggatcggcgc aaagggttga gccagccgcc
     2701 ctttgcgccg atggttaggt gagggcaaat ctcccgcgcc tatggcgcag gcgtgggcgt
     2761 ggtcaactgt ccggttgacc agtcgtagaa ctggtaa
//
LOCUS       DAOPVS010000214         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_37424_length_2794_cov_58.302665, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000214 DAOPVS010000000
VERSION     DAOPVS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37424_length_2794_cov_58.302665"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..826
                     /locus_tag="PKL11_10790"
     CDS             83..826
                     /locus_tag="PKL11_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14128.1"
                     /translation="MEELSTLTNILVDALIYLANGGLLLVYLLASNLPAIVSLASAGF
                     VFLVMDSEVQRRAGFRPQRGGPMGQPGLPERRSVLAQAMDNEGSAGAQPRTAQILTGI
                     VFALWMIAQTGMAAPVPWIGAAMWLVGVIVLLVMPNHQRFNQLWFVKTGLAVYALLVI
                     GSRIYLAYATQLSPEQWAGIIGTAESAAAIIANTKGNVTTIILWALWLVAPLGYFSLL
                     VQQLFVNPMNLVSPLAGAQDVLKRIRERV"
     gene            1118..1471
                     /locus_tag="PKL11_10795"
     CDS             1118..1471
                     /locus_tag="PKL11_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14129.1"
                     /translation="MYDLNLNSVCFVGPFPDWAGQVAIALVVALVALVITLTVVMATT
                     AARIAGSFRKTGKLPESLMKFAEARQPLREHKHIHYSVAATEQMEREQQAAATGPIPL
                     DEAGYDFYGIDSGMN"
     gene            1596..>2794
                     /locus_tag="PKL11_10800"
     CDS             1596..>2794
                     /locus_tag="PKL11_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14130.1"
                     /translation="MVIAALKDIRIPWLTEKTGKKSSQPLIPDDLQLMDNGIAFNEVN
                     DTLDYLWYAVEEKDNGRVYRGFRVVRLLELKFIPLDARADAGLLQKMKSVLRSLYGAK
                     VSFVYLTAGIFTDPAVGIIQCYGVSVFAKDLEEAAKLSANALSALKSAMTGAFRQMKL
                     SPLTPETGQWIFGAMADMKHALVVVGQPDPRENAKGGSQALFNNPLTTGNSAAQQYSL
                     QQNEILFRGMSTLKEDFLFLVMTSPIALSDISEMLIGLAESTSTWASWQNGVRGASFG
                     ISLPAILSGALMQSSAQGFGESEGTSHSDGTAHSTGAAHTEGEAHTEGSAYTVGRTHS
                     VGESTTVTSGVATSDIHSVSEGEAHSESSGVADGTSSSSGTSGSHTQSSGQSDSVGVG
                     VNAGASLG"
BASE COUNT          636 a          755 c          827 g          576 t
ORIGIN      
        1 ttcgcagtcg ttatagatta agacggctaa tgtatggccg tttttcgttt taacctcccg
       61 gaaaggagcc gggcaagccc ttatggaaga attatcaacc ctgacgaata ttcttgtaga
      121 tgcgctgatc taccttgcca acggcgggct gctgctggtt tacctgctgg cgagcaacct
      181 gccggcgatt gtaagcctgg catcagcggg ctttgtcttt ctggtcatgg acagtgaagt
      241 gcagcgtcgg gcaggctttc ggccacagcg cggcggcccg atggggcagc ctggactgcc
      301 ggaacgccgg agtgtgctgg cgcaggcaat ggacaacgaa ggctcagccg gagcgcagcc
      361 gcgcaccgct caaattctga ccgggattgt ctttgccctg tggatgatcg cccaaaccgg
      421 gatggcagcg ccggtgccct ggattggagc ggccatgtgg ctggtgggcg tcattgtcct
      481 gctggtcatg ccgaatcatc agcggtttaa ccagttatgg tttgtgaaga cgggtctggc
      541 ggtgtacgcc ctgctggtga ttggcagccg aatctacctg gcgtatgcca cccagctatc
      601 gccggaacaa tgggccggca tcattggtac tgcagaaagc gcagctgcga ttattgccaa
      661 caccaaaggc aacgttacca cgatcatcct gtgggcgttg tggctggtag cccctttggg
      721 atacttctcg ctgctggtgc agcagttatt tgtcaacccg atgaacctgg tcagcccgct
      781 ggccggcgcg caggatgtcc tgaagcggat tcgagaacgt gtgtaactga acttttacaa
      841 ccgcccttgt ggatgatggt aaggcaggtt cgactcctgc cggggcattt tatatctgac
      901 ctgaatctcc ccaaaaaagc gcatcggatc ggacctgaac tgatccgagt agcaacagga
      961 gatcggcaga tacataaagt tggaatgcct ggtttctgca aagaacaccc ggcgaaataa
     1021 gcaagaaaac aatttttatc cctcaacaag tgaccaacgg gaaaggcggc cagaagcgca
     1081 agcgcccgga ggtcaaaaca aatcacaagg agaaaaaatg tacgacctga accttaactc
     1141 tgtatgtttt gttggcccat tccccgattg ggccggccag gttgccatcg ctctggtggt
     1201 ggcactggtg gcactcgtga tcaccctgac agttgtaatg gcgaccacgg ctgcgaggat
     1261 tgctggcagc ttccgtaaaa ccggcaagct cccggaatcg cttatgaaat tcgctgaggc
     1321 tcgtcagcct ctgcgggaac ataagcacat ccattactcg gtggcggcca ccgagcagat
     1381 ggagagggaa caacaggccg ccgctaccgg accaattcct ctggatgagg ccgggtacga
     1441 tttctacggg attgacagcg gcatgaacta gccgcaatca atccacctcc caaaaactac
     1501 gtcaccccca tgcggggtgg cgcagggggg cggctcacga aagtggacct tccccctgcg
     1561 ctacccggcg cgaaagaaaa aggagttttg aaccaatggt aatagccgcg ctcaaagaca
     1621 ttcgaatacc ctggctgact gaaaagacgg gaaagaagtc aagtcagccg ttgattccgg
     1681 atgacctgca actgatggac aacgggatcg ccttcaacga ggtcaacgac acactggatt
     1741 atctctggta cgccgtcgaa gaaaaggata acgggcgggt gtatcgcggt tttcgggtgg
     1801 tgcggctgct ggaactgaaa ttcatccccc tggatgcccg cgctgacgcc ggccttttac
     1861 agaagatgaa atccgtcctg cgctcattgt acggagcaaa ggtatcgttt gtgtacctga
     1921 ctgccggcat cttcaccgat cctgcggtgg gcattatcca atgctacggt gtctcggtat
     1981 ttgcaaaaga ccttgaagaa gcggccaaac tttcggcaaa cgccctgagc gcgttgaaat
     2041 cagccatgac cggtgcgttc cggcagatga aattatcccc cctgactcct gaaaccggcc
     2101 agtggatttt tggcgcaatg gctgacatga aacacgccct ggttgtcgtg gggcagccag
     2161 atccgcgcga gaacgccaaa ggcggcagcc aggcattatt caacaatccc ctgaccacag
     2221 gaaacagcgc cgcgcagcag tacagcttgc agcagaacga aatcctgttt cgcggcatga
     2281 gtacgctgaa agaggacttt ctattcctgg taatgacttc cccaattgcc ctgtccgaca
     2341 tcagcgaaat gctgatcggc ctggctgaga gtaccagcac ctgggcctcc tggcagaatg
     2401 gtgtgcgggg agcctcgttt gggatcagcc tgccggcgat attgagcggc gcattgatgc
     2461 agtcatcggc gcagggcttt ggggagagcg aaggcacgtc gcatagcgac ggtacagcac
     2521 actcgactgg tgcggcccat acggaaggcg aggcgcatac tgaggggtct gcgtacaccg
     2581 taggccggac gcactcggtt ggcgagtcca ccaccgtcac cagcggcgta gccacttctg
     2641 atattcattc cgtttcagaa ggcgaggccc attctgaaag cagcggtgta gcggatggca
     2701 cgagttcctc tagcggcacc agcgggagcc atacacagtc cagcggtcag tcagatagcg
     2761 ttggcgttgg tgtgaatgcc ggtgcaagcc tggg
//
LOCUS       DAOPVS010000215         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_37584_length_2785_cov_46.376190, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000215 DAOPVS010000000
VERSION     DAOPVS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37584_length_2785_cov_46.376190"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PKL11_10805"
     CDS             complement(<1..1142)
                     /locus_tag="PKL11_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ14131.1"
                     /translation="MYTDPINVDLEARRCLRRILPGMESKLAGQIAADPQMWAIFKER
                     LGLHLPALFRLYHHLYARRYDFFYHFENLLYSLADAAFSRSEVLRQLDQEREAQPDWF
                     QSNQMVGGVCYVDLFADDLIGLKSKIPYFKELGLTYLHLMPLFKMPEGENDGGYAVSS
                     FREVHAPLGSMQQLASLAHELREQGISLVLDLIINHTSDEHEWVQQAKTGDPDYREMY
                     GIFPDRTGPDAYEKHLRDIFPDEHPGAFTWSEAMQSWIWTTFHSYQWDLNYANPMVFN
                     KMAEEFLFLANQGIEIFRLDAVAFIWKQLDTGCENLPEAHMILQGFNALTRIAAPAVL
                     FKSEAIVHPDDVVKYISPQECQLSYNPLLMALLWNTLATRDIGLLA"
     gene            complement(1155..>2785)
                     /gene="treS"
                     /locus_tag="PKL11_10810"
     CDS             complement(1155..>2785)
                     /gene="treS"
                     /locus_tag="PKL11_10810"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNZ14132.1"
                     /translation="KGYLMSTSLPWYKTAVFYELYVRAFNDSNGDGWGDLQGVIEKLD
                     YLRDLGVDCIWLLPIAPSPLRDDGYDVSDYCDIHPMYGKMDDFRRLVKAVHARGLRLL
                     VELVPNHTSDQHAWFQASRDPKHPEHETYKDYYVWSETPERYQGARIIFLDTEKSNWS
                     YDPVRKAYYWHRFFSHQPDLNYDNPAVQRAMLRTIQFWIDEGVDGIRVDAPPYLFERE
                     GTNCENLPETHLFLKRLRAFVEAYAPGTMLLSEANQWPEDVIHYFGTGDEMHMNFHFP
                     LMPRLYMALAKADRAPIEAILERTPALPEVCQWGTFLRCHDELTLEMVTPDERQWMWE
                     TYAPDARMRLNLGIRRRLAPLMNNDPRKIMLLNSMLFTMGGSPFLYYGDEIGMGDNIW
                     LDDRNGVRTPMQWHDGLNGGFSNAAPEKLYAPVISAAPFEYATVNVARQEHNPESLLN
                     KTRHMIATRRQHPVFGAGSFRWADVGSDAVAAYWREYQESQMLVLNNLSEQAMTVSIE
                     TRPGGYVNVLDQKPFNGQQIRLEPYQYLWLAPQG"
BASE COUNT          633 a          838 c          681 g          633 t
ORIGIN      
        1 tgcgccagca agccgatatc gcgggttgcc agggtattcc acagcagcgc catcagcagg
       61 gggttataag agagctggca ttcctggggt gaaatgtatt taaccacatc atccggatgc
      121 acaatcgctt cagacttgaa caacactgcc ggagcagcaa tccgcgtcag cgcgttgaac
      181 ccttgcaaaa tcatgtgcgc ctctggcagg ttttcacatc ccgtatccag ttgcttccag
      241 ataaacgcca ccgcatccag cctgaagatt tcaatccctt gatttgccag gaacagaaat
      301 tcttcggcca tcttattaaa gaccatcggg ttggcataat tcaaatccca ctggtaagag
      361 tggaacgtcg tccaaatcca gctttgcatc gcttcagacc aggtgaacgc tcccggatgt
      421 tcgtccggga aaatatcccg caaatgtttt tcgtaggcat cgggccccgt ccgatccggg
      481 aagatgccgt acatttcccg gtaatccggg tcgccggttt tggcttgctg cacccattca
      541 tgctcgtccg aggtgtgatt gataatcaaa tccagcacca ggctgatgcc ctgttcgcgc
      601 agttcgtgtg ccagactggc cagttgctgc atactgccca gcggcgcatg aacctcccga
      661 aagctgctga cggcataacc gccatcattt tcgccttctg gcatcttgaa caagggcatc
      721 aaatgcaggt atgtcagccc cagctctttg aagtagggga tcttgctctt cagcccgatc
      781 aggtcatctg caaacagatc cacatagcag accccgccca ccatctggtt gctctgaaac
      841 caatctggct gggcttcccg ctcctggtct agctgcctga gcacctcgct gcgcgaaaat
      901 gccgcatcag ccaggctgta gagcaaattc tcgaaatgat aaaagaaatc ataccgccgg
      961 gcatacaggt gatgatacag ccgaaacagc gctggcaggt gcaggcccag ccgctctttg
     1021 aatattgccc acatctgcgg gtcagcggca atctgcccgg ccaattttga ttccatcccg
     1081 ggcaaaatcc gccgcaggca gcgcctggct tccagatcaa cattaatcgg atcggtgtac
     1141 atgagtccgc tcctttaccc ttgcggggca agccatagat actgatacgg ttccagtcgg
     1201 atttgttggc cattaaacgg cttctgatcc agcacattca catacccgcc cggtctggtt
     1261 tcgatactga cagtcattgc ctgctccgat aaattgttca aaaccagcat ctggctttcc
     1321 tgatattcac gccagtaagc cgccaccgcg tctgacccca catccgccca gcggaagctc
     1381 cccgcgccaa acaccgggtg ctgccttcgg gtggcaatca tatggcgggt cttatttagc
     1441 agcgattccg ggttatgctc ctgtcgagcg acattgactg tggcatattc gaagggcgcc
     1501 gcactaatta ccggggcata cagtttttct ggcgcggcat tagaaaagcc gccattcagc
     1561 ccatcgtgcc actgcatggg ggtgcgtacc ccattgcggt catccaacca gatattatcc
     1621 cccattccaa tctcatcgcc atagtacaaa aatggcgacc cgcccatggt gaaaagcatc
     1681 gagttcaaca gcataatctt gcgtggatca ttattcatta gcggcgccaa ccgccgccgg
     1741 atgcccaggt tcagccgcat ccgcgcgtcc ggtgcatagg tttcccacat ccactggcgc
     1801 tcatcgggcg tcaccatctc cagcgttagc tcatcatggc agcgcaggaa cgtgccccac
     1861 tggcatacct cgggcagcgc cggtgttcgt tctaaaatcg cctcaatcgg ggctcgatct
     1921 gccttcgcca gtgccatgta gagccggggc atcagtggga aatggaaatt catatgcatt
     1981 tcatcccctg tcccaaaata gtgaatcaca tcctctggcc actggttggc ttctgaaagc
     2041 agcatcgtcc ccggtgcata agcctccaca aatgcgcgca aacgcttcaa aaacaagtga
     2101 gtctctggca ggttttcgca gttggtgcct tcccgttcaa acaggtacgg cggcgcatcc
     2161 acgcgaattc catccacgcc ctcatcaatc caaaactgaa tggtgcgcag catcgcgcgc
     2221 tgcaccgcgg gattatcgta gttcaaatct ggctggtggc tgaaaaagcg gtgccagtaa
     2281 taggccttgc gcaccgggtc gtagctccag ttcgattttt ccgtatccag aaagatgatc
     2341 cgggcgccct ggtagcgctc tggcgtctcg ctccagacat aataatcttt atacgtctcg
     2401 tgttccgggt gcttggggtc gcgtgaagcc tggaaccagg catgttggtc tgaggtgtgg
     2461 tttggcacca actccaccag cagccgcaaa ccccgggcat gcaccgcctt gaccagccgc
     2521 cgaaaatcgt ccatcttgcc atacattgga tgaatatcac agtaatcgct cacatcatac
     2581 ccatcatcgc gcagcggtga tggcgcaatc ggcagcagcc agatgcagtc cacccccaga
     2641 tcacgtaaat aatccagctt ttcaatcaca ccctgcaaat ccccccagcc gtcgccgtta
     2701 ctgtcgttga atgcgcgtac atataactcg taaaacactg ctgtcttata ccaggggaga
     2761 gaggtgctca taagatatcc tttcc
//
LOCUS       DAOPVS010000216         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_37847_length_2772_cov_39.994847, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000216 DAOPVS010000000
VERSION     DAOPVS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37847_length_2772_cov_39.994847"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKL11_10815"
     CDS             <1..784
                     /locus_tag="PKL11_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNZ14133.1"
                     /translation="PLPTQRKIAAILSAYDDLIENNTRRIRILEAMAQAIYREWFVNF
                     RYPGHEGVRLIESPLGPIPEGWEVKKLGDLVGCIRKSTEPGPHLEELRYIPIDLIPRK
                     SLSIIESLPGDQAKSSLLMFDENDVLFGAMRPYFHKVSIAPFNGVTRSTCFVLRPIEP
                     CYLAYSLMTLFQTSTIEFATNHSQGGTIPYVVWDNSMSEMPVINPPISVINPFEQILH
                     PIIWTFKRHYFRQNNLRQQRDLLLPRLISGELDVEALEIPTL"
     gene            824..>2772
                     /locus_tag="PKL11_10820"
     CDS             824..>2772
                     /locus_tag="PKL11_10820"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNZ14134.1"
                     /translation="MDYSEDRLIEQPAIARLQSLGWQAANCYQESFGPQGSLGRQTSA
                     EVVLQPRLLAALRRLNPDLPESLLALAADELSRDRSALSPVEANRQVYLLIRHGVLVT
                     ARDEDGAERTERVQVIDWTNPQNNDFFLASQLWISGEVYKRRADLIGFVNGLPLVFIE
                     LKAAHKAVKAAYDGNLRDYKNTIPQLFWYNAIIILSNGSESRIGSLSATWEHFAEWKK
                     INSEGEEGLISLETMLRGVCDPEKLLDLIENFTLYEDAKGGLRKIIAKNHQMLGVNAA
                     FQSIQQIRENQGKLGVFWHTQGSGKSYSMVFFSQKVLRKLPGNWTFLVVTDRVELDDQ
                     IYKNFANCGVVTEPEHSVRASSGDHLQDLLREDHRFLFTLIQKFRIDNGHTYPQLSER
                     RDIIVMTDEAHRSQYDVLALNMRNALPNAAFLAFTGTPLMAGEEKTRQVFGDYVSIYN
                     FKQSVDDQATVPLYYENRIPELQLTNPNLNQDMADLLDDADLDEGQESRLEREFSREY
                     HLITRNDRLEKIAADIVQHFLGRGFPGKAMVISIDKATAVKMHDKVRAHWARELAALE
                     AELETCADEDRPALQARRDYMKSTDMAVVVSQGQNEEEDLRAKGAEIMQHRRRMQQED
                     LATKFKDPSDPFRIVFVCAMWMTGF"
BASE COUNT          645 a          795 c          788 g          544 t
ORIGIN      
        1 ccctctcccc acgcagcgga agattgcggc gattctgtcg gcgtatgacg atttgattga
       61 gaataacacg cgccgcatcc gtattctgga ggcgatggcg caggcgattt accgcgagtg
      121 gtttgtgaat tttcgctacc ccgggcatga gggggtgcgc ctgatcgagt cgccgctggg
      181 tcctatcccg gaggggtggg aggtaaaaaa actcggtgac ttagtgggtt gcattagaaa
      241 atctacagaa ccaggtcccc acctagaaga acttcgctat attcctattg acttgatacc
      301 cagaaagagt ttatccatca tcgaatcatt gccaggagat caggcaaaaa gcagcttact
      361 tatgttcgat gaaaacgatg tattgtttgg cgcaatgagg ccatattttc acaaagtatc
      421 tatagcacct tttaatgggg tcacaagaag tacatgcttt gtattacgac caattgaacc
      481 atgttacctt gcatattcat taatgacact attccaaact agtactattg aatttgccac
      541 caatcactct cagggcggaa caattcctta tgttgtttgg gacaattcaa tgtcggagat
      601 gcctgtaatc aaccctccta tctccgttat caatcctttt gagcaaatcc tgcatcctat
      661 tatttggact ttcaagaggc attattttag acaaaataat cttcgccaac agcgcgacct
      721 gctgctgccg cggctgatca gcggcgagct ggacgtggaa gccctggaga tccccaccct
      781 gtaagcggcg cctaccccct ttttgcggca gagagaaaaa gccatggact attctgaaga
      841 ccgcctgatt gaacaacccg ccattgcccg gctgcaaagc ctgggctggc aggcggctaa
      901 ctgctaccag gaaagctttg gcccccaagg cagcctgggc cggcaaacct cggccgaggt
      961 ggtgctgcag ccccggctgc tggcggcgct gcgccggctg aaccccgacc tgcccgagag
     1021 cctgctggcg ctggcagccg atgagctgag ccgtgaccgc agcgcgctga gcccggtgga
     1081 agccaaccgc caggtgtacc tgctgatccg gcacggggtg ctggtgaccg cccgggatga
     1141 agacggcgcc gagcgcaccg agcgcgtgca ggtgattgac tggaccaacc cccaaaataa
     1201 cgacttcttc ctggcctcgc agttgtggat ctcgggcgag gtgtataagc gccgggcaga
     1261 cctgattggt tttgtcaacg ggctgccgct ggtcttcatc gaactgaagg cagcgcataa
     1321 ggcggtcaaa gccgcgtacg acggcaacct gcgggactat aagaacacca tcccgcagct
     1381 cttctggtac aacgccatca tcatcctgtc taacggcagc gaaagccgca ttggcagcct
     1441 ttcggccacc tgggagcatt ttgccgagtg gaagaaaatc aacagcgagg gggaagaggg
     1501 cctcatctcg ctggagacca tgctgcgggg cgtgtgcgac cccgaaaaac tgctcgacct
     1561 gattgagaac tttaccctgt atgaagacgc caagggcggg ctgcgcaaga tcatcgccaa
     1621 gaatcatcaa atgctggggg tcaacgccgc cttccagtcc attcagcaaa tccgcgagaa
     1681 tcagggcaag ctgggggtct tctggcatac gcagggcagc ggcaaatctt actcgatggt
     1741 gtttttctcg cagaaagtgc tgcgcaaact gcccggcaac tggacctttc tggtggtgac
     1801 cgaccgggtg gaactggacg accagatcta caagaatttt gccaactgcg gcgtggtcac
     1861 cgagccagag cacagcgtgc gcgccagcag cggcgaccat ctgcaagacc tgctgcggga
     1921 agatcaccgc ttcttgttca ccctgattca gaaattccgc attgataacg gacacaccta
     1981 cccgcagctc tcggagcggc gcgacatcat tgtgatgacg gatgaggccc accgcagcca
     2041 gtacgacgtg ctggcgctga acatgcgcaa cgccctgccc aatgcggcct tcctggcctt
     2101 tacgggcacc ccgctgatgg ccggcgaaga aaagacccgc caggtctttg gcgattacgt
     2161 cagcatctac aacttcaaac aatcggtgga cgaccaggcc accgtgccgc tgtattacga
     2221 aaaccgcatc cccgaactgc agctcaccaa ccccaacctc aaccaggaca tggccgacct
     2281 gctggatgac gccgacctgg acgaagggca ggagagccgg ctggaacggg agttcagccg
     2341 ggaatatcac ctgatcaccc gcaacgaccg gctggagaag attgccgcag acattgtgca
     2401 gcacttcctg gggcggggct tccctggcaa ggccatggtc atctctattg ataaggccac
     2461 cgccgtcaag atgcacgaca aagtgcgcgc ccactgggcg cgggagctgg cagccctgga
     2521 agccgaactg gagacctgcg ccgacgaaga ccgcccggcg ctgcaagccc ggcgggatta
     2581 catgaagtcc accgatatgg cggtggtggt ttcgcagggc cagaacgaag aggaagacct
     2641 gcgggccaaa ggcgccgaga ttatgcagca ccgccggcgg atgcagcagg aagacctggc
     2701 gaccaaattc aaagacccca gcgatccctt ccggattgta tttgtctgtg cgatgtggat
     2761 gaccggcttt ga
//
LOCUS       DAOPVS010000217         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_38162_length_2757_cov_58.873797, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000217 DAOPVS010000000
VERSION     DAOPVS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38162_length_2757_cov_58.873797"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKL11_10825"
     CDS             complement(<1..271)
                     /locus_tag="PKL11_10825"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNZ14135.1"
                     /translation="MATANTCCAWLKKPSGGSTMRILLFGKTGQLGWELQRTLPCLGE
                     VTALDYPEVDFSHPESLRTVVRSAAPDVVINAVAYTNVDKAEIEPE"
     gene            complement(219..1097)
                     /gene="rfbA"
                     /locus_tag="PKL11_10830"
     CDS             complement(219..1097)
                     /gene="rfbA"
                     /locus_tag="PKL11_10830"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNZ14136.1"
                     /translation="MKGIILAGGKGTRLYPITLAVSKQMLPVYDKPMIYYPLSMLMLA
                     NIREILVISTPEDLPGFRRLLGSGEQWGLKFEYAEQAQPRGLAEAFIIGQKFIDNQPA
                     CMILGDNIFYGHGLPAKLSQAAQITEGATVFAYPVRDPERYGVVDFDENFKALSIEEK
                     PKNPRSNYAVPGIYFYDQEVSRIAASLKPSPRGELEITDLNRIYLEQGKLRVEVLGRG
                     VAWLDAGTHESLLQASTFVQTVEERQGMMISCPEEIAYRMGFITLDQLRQQGELMAGN
                     GYGQYLLRLAQEALRR"
     gene            complement(1094..2176)
                     /gene="rfbB"
                     /locus_tag="PKL11_10835"
     CDS             complement(1094..2176)
                     /gene="rfbB"
                     /locus_tag="PKL11_10835"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNZ14137.1"
                     /translation="MKNVLVTGGAGFIGSNFIRYLLKQDPAVQIVNLDALTYAGSLEN
                     LNDLPDFNRHHFVQGDICDRSLVDDLLHQYQIDTIVHFAAETHVDRSILGPGQFIQTN
                     IIGTFTLLEAARQMWLTEKLLPLEGVRFHHVSTDEVFGTLEPNDPPFCETTPYSPNSP
                     YSASKAASDHLVRSYWHTYGLPISITNCTNNYGPAQFPEKLIPLMVLNARDGKPLPVY
                     GDGMQIRDWLYVEDHCEAIHAVITRGQPGETYAVGGNNQPPNIEIIHTICDIMDELQP
                     GEHSPHASLIKHVTDRPGHDRRYAMDIAKIQTELGWQPRQSLKTGLLHTVRWYLDHPE
                     WVTAVHQQSDLQSWMAKNYGNRGGNQ"
     gene            complement(2192..2740)
                     /gene="rfbC"
                     /locus_tag="PKL11_10840"
     CDS             complement(2192..2740)
                     /gene="rfbC"
                     /locus_tag="PKL11_10840"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNZ14138.1"
                     /translation="MKFVATDLPGVVIVEPQVFGDARGFFMETYQECVFAEAGLPTHF
                     VQDNHSGSRQGILRGLHYQIRQPQGKLVRAVAGEVFDVAVDLRKSSPYFGKWFGLVLS
                     AENKRMLWVPPGFGHGYYVLSEWAEFVYKTTDFYAPQWERTLRWDDPQVGVDWPLVAG
                     QPPLLSTKDAQGAALAEAEVFE"
BASE COUNT          561 a          740 c          868 g          588 t
ORIGIN      
        1 gttcaggctc gatctcagct ttatccacat tggtataggc cactgcgtta atcacaacat
       61 cgggggcggc ggagcgcacc acggtgcgca agctttcggg gtgagaaaaa tcgacctcgg
      121 ggtagtccag ggcagtgact tcgcccaggc agggcaaagt gcgctggagt tcccagccga
      181 gctgaccggt tttgccaaag agcaggatgc gcatggtgct accgccggag ggcttcttga
      241 gccaggcgca gcaggtattg gccgtagcca ttgccagcca tcagttcgcc ctgctggcgc
      301 agttggtcga gggtgatgaa gcccatgcgg taggcgattt cttcggggca ggagatcatc
      361 ataccctggc gttcttccac cgtctggacg aatgtggaag cctgcagcaa cgattcgtgc
      421 gtaccggcgt ccagccaggc cacgccgcgc cccagcactt cgacgcgcag tttgccctgc
      481 tcgaggtaaa tgcggttgag gtcggtaatc tccagttcgc cgcgcggcga gggtttgagg
      541 ctggccgcaa tgcggctgac ttcctggtca tagaaataga tgccgggaac ggcatagttg
      601 gagcgcgggt tcttgggttt ttcttcaatg ctgagggcct tgaagttctc atcgaagtcc
      661 accacgccgt aacgctcggg atctcgcacg gggtaggcga agacggtggc gccctcggtg
      721 atctgcgccg cctggctgag tttggcgggc aggccgtgtc catagaagat gttatcgccc
      781 aggatcatgc aggcgggttg attatcaatg aatttctgac cgatgatgaa ggcctcggcc
      841 aggccgcggg gctgggcttg ctcggcatat tcaaatttga gcccccactg ttcgccgctg
      901 cccagcaggc gccgaaaacc gggcaggtct tcgggtgtgc tgatgaccag aatttcgcga
      961 atgttcgcca gcatcagcat ggacaggggg tagtagatca tcggcttgtc atagacgggc
     1021 agcatttgtt tgctgaccgc cagggtgatg gggtacaggc gggtgccttt gccgccagca
     1081 agaataatgc ctttcattgg ttgcctcccc gattgccata atttttcgcc atccagcttt
     1141 gcaggtccga ctgctgatgg acagcggtca cccattcggg atggtcgaga taccagcgca
     1201 ctgtgtgcag cagcccggtc ttcaaagatt ggcggggctg ccagcccagt tcggtttgga
     1261 ttttggcgat atccatggca tagcggcggt catgtccggg gcggtcggtc acatgtttga
     1321 tcagagacgc atgcggtgaa tgctcgccgg gttgaagttc gtccatgata tcgcaaatgg
     1381 tgtggatgat ttcaatattt gggggctggt tgttgccgcc tacggcgtag gtttcgccag
     1441 gttggccgcg ggtaatcacg gcatggatcg cctcgcagtg gtcttcaaca tacagccagt
     1501 ctcggatttg catgccgtcg ccataaacgg gcaagggctt gccatcgcgg gcgttcaaca
     1561 ccatgagggg gataagtttt tcggggaact gcgccgggcc atagttgttg gtgcagttgg
     1621 taatgctgat cggcaggccg taggtgtgcc agtatgaacg caccagatga tcggatgccg
     1681 ccttagaggc agaatagggc gagttgggtg agtagggcgt ggtctcgcag aagggcgggt
     1741 cgttgggctc gagggtgcca aagacttcgt cggtggacac atggtggaag cgcacccctt
     1801 ccagcgggag gagcttctcg gtcagccaca tctggcgggc ggcttccagc agggtgaacg
     1861 tgccaatgat gttggtctgg atgaactgcc cggggccgag gatggagcgg tccacatggg
     1921 tttcggcggc aaagtggacg atggtatcaa tctggtactg gtggagcaaa tcatccacca
     1981 gggagcggtc gcagatatcg ccctggacga agtgatgccg gttgaagtct ggcagatcgt
     2041 tcaggttttc caggctgccg gcgtaggtca gggcatccag attgacgatc tgaacggcag
     2101 gatcctgctt gagcagataa cggatgaaat tggagccgat gaacccggcg ccacctgtga
     2161 ccagcacatt tttcatagaa tactcctcgg tttattcaaa aacttcggct tcagccaggg
     2221 ctgccccctg ggcatctttg gtcgaaagca gtggaggctg cccggccacc aaaggccaat
     2281 ccacgcccac ctggggatca tcccaacgca gggtgcgttc ccactgcggg gcgtaaaaat
     2341 cggtggtttt atagacaaat tcagcccact cactgagcac atagtagccg tgcccaaaac
     2401 cgggcggcac ccataacatg cgtttatttt ccgccgagag caccaggccg aaccatttgc
     2461 caaaataggg cgagcttttg cgcaggtcaa ctgccacgtc gaaaacttca cctgccacgg
     2521 cgcgcaccag tttgccctgc ggctgccgga tctggtaatg caggccgcgc aagatgccct
     2581 ggcggctgcc cgagtgatta tcctggacaa agtgagtggg cagacccgcc tcggcaaaga
     2641 cgcattcttg ataggtttcc ataaaaaagc cgcgcgcatc gccaaagact tgaggctcaa
     2701 caatcacaac ccccggcaga tcagtcgcca caaacttcat actgcatcac cctcacc
//
LOCUS       DAOPVS010000218         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_38559_length_2737_cov_49.990306, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000218 DAOPVS010000000
VERSION     DAOPVS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38559_length_2737_cov_49.990306"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..593)
                     /locus_tag="PKL11_10845"
     CDS             complement(36..593)
                     /locus_tag="PKL11_10845"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ14139.1"
                     /translation="MSDTHLIYPDNVIQTHRFQYCPICQAPLKRDALFADNLLHTHCA
                     ACGWIQLVSNCICVAAVVRHNGGIVVIQPPDGDGAGFPAGIVEYGENPQDTIIRETLE
                     ETGLVVEIVRDLGWSYVSYEDFPGPTVYILFEVQSVGGELKEGDEGPVQVYALKDLPQ
                     ISPKRHGSYMARKRFLEQMDKAEKA"
     gene            811..1104
                     /locus_tag="PKL11_10850"
     CDS             811..1104
                     /locus_tag="PKL11_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14140.1"
                     /translation="MPGDEEISTVYDEGKEAVVTLFHRMVGLLAARVQTLEDWVSNNN
                     RNNGKSPSSDRLPKPRVEVTEHQVKIKRYPHCGAENRAPFPEGMLIDYACHNY"
     gene            1137..1928
                     /locus_tag="PKL11_10855"
     CDS             1137..1928
                     /locus_tag="PKL11_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNZ14141.1"
                     /translation="MTQLAATYSPYLHELLGQKKVSLDAIEFTPWQSISSLPKILPDL
                     TDYLSYFHGSNLTARMALLPGSRLKLSKALHLTRARWFSTHISLLPPGWTWLSLRFGI
                     YLPPLPVEWLLSRFIRNVERLKSEYSSIPLLLENMPVEPRRVYKHLKSPVLIREVLEK
                     TGCGLLLDLPHARIAAEAFGMETTEYIQQLPLDRVVEIHTSGPGRQQNGTLIDKHCEM
                     LSEDYELLNWASAQTPVQLVTLEYIKDQDGLYRQLLKLSHLIRQK"
     gene            2066..2653
                     /locus_tag="PKL11_10860"
     CDS             2066..2653
                     /locus_tag="PKL11_10860"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNZ14142.1"
                     /translation="MTEIIAADFYNREVVTVARDLLGLRLVRRWQGQRLSGVIIETEA
                     YRGEDDLACHAHRGQTKRNAVMYGPPGHAYIYFTYGMHWCLNCVTGPAGYPAAVLIRA
                     LLPVEGLEQIAAHRAGVKPALWCAGPARLTRALAIDGALNGCDLTSPPGDLWIESAAL
                     PATAEIVTGPRVGINYAPEPWRSMEWNFKLVEVHP"
BASE COUNT          662 a          719 c          686 g          670 t
ORIGIN      
        1 cataggttca ttatacaggc ggtttggggc gctgattaag ctttttcagc tttgtccatt
       61 tgctccagaa atctctttcg agccatatag ctgccatgac gtttgggtga aatctgtggt
      121 agatctttga gggcatacac ctgaacaggg ccctcatcgc cttctttgag ttctccgcca
      181 acagattgca cttcaaagag gatataaacc gtggggccgg gaaagtcttc ataagagaca
      241 tacgaccagc ccaaatcacg cacaatttca accaccagcc cggtttcttc gagcgtttct
      301 cgaataattg tgtcctgtgg gttttcgcca tattccacaa ttccagcggg gaaccccgcg
      361 ccatcgccat cgggcggttg aatgaccaca atgccgccgt tgtgcctgac gactgcagcc
      421 acacagatgc agttcgaaac aagttgaatc cagccgcagg ctgcgcagtg ggtatgcagg
      481 aggttgtcag caaatagcgc atctcttttg agtggagcct ggcatattgg gcagtattga
      541 aaacgatgcg tctggatgac gttatccggg tagatcaaat gggtatctga catggttcct
      601 caaactgatc atacagaaga atgggataca gcaatcatac cgtaattaac ctcaaagcag
      661 aagttttgga ttcattccac gggagttgaa gggacgcgaa gatggcataa ataatctttg
      721 tttgcaactg ttcaagcata aatctaggcg cccataaaca ttgtcaaaat cgccaaacat
      781 aagcacaatt ataggatgga gccattccaa ttgcccggtg atgaagaaat aagcacggtg
      841 tacgatgaag gtaaagaagc agtagttacg ttatttcatc gaatggtagg cctattagct
      901 gcgcgggtac aaacattgga agattgggtt tcaaacaaca atcgcaacaa tggcaagtca
      961 ccatccagtg atagattacc taaaccacga gtggaggtga cggaacatca ggtgaagatc
     1021 aagcgttatc cacattgcgg ggccgaaaat cgcgcacctt ttcctgaagg gatgttgatt
     1081 gattacgctt gccataacta ttgaacgctt accaccctga gagaaggatc ggttaaatga
     1141 cccaactagc tgcgacatac tctccttacc ttcacgaact actcggccaa aagaaagttt
     1201 ccttggatgc tattgagttt actccctggc agagtatctc ttcgctgccg aaaattctac
     1261 ctgatctgac tgattattta tcgtatttcc acggatccaa tttgacagcc cggatggcgc
     1321 tgttacctgg ttcacgcttg aagctctcga aagctctcca cctaactcgc gccagatggt
     1381 tttcgaccca tatttctctc ttgccgcccg gttggacatg gttgtcttta cgctttggta
     1441 tttatttacc acctcttcct gtagaatggt tgttgtccag gtttattcgt aatgtcgagc
     1501 gtttaaagag tgaatatagt agcatccctc tacttctgga aaacatgccg gttgaaccgc
     1561 gccgcgtcta caaacacctg aagagtcctg tacttatccg agaagttcta gagaaaaccg
     1621 gttgtgggct tctccttgat ttaccccatg cgcgtattgc ggcggaagca tttggtatgg
     1681 aaacaacaga atatatccag caactcccac ttgaccgggt tgttgagatc catacgagcg
     1741 gacccggacg tcagcagaac ggaaccttga tcgataaaca ttgtgaaatg ctttctgaag
     1801 attacgaact actcaattgg gcatcggctc aaactcctgt gcagttggtg acacttgaat
     1861 atatcaagga ccaggacggc ttgtataggc agctattgaa actttcccat ctgataagac
     1921 aaaagtaaat tcatagcttc attaatttac agtctggcta gttacctttg tttttattgc
     1981 acaaccttct gtaagcacag ccacaatcct acgctttttg aggattaccg ttcaggggcc
     2041 gagttgggcg tggtagaatc gacgtatgac cgagatcatt gcggcagatt tttacaatcg
     2101 ggaagtggtg acggtggcgc gagacttgct gggcctgcgt ctggtacgcc gctggcaggg
     2161 gcagcgcctc agcggggtga ttatcgagac cgaggcctac cgcggcgaag atgacctggc
     2221 ctgccacgct caccgcggtc agaccaaacg caacgcggtg atgtacggcc cgcccggaca
     2281 tgcctacatt tacttcacct acggcatgca ctggtgcctc aactgcgtca ccggcccggc
     2341 gggctacccc gccgccgtgc tgattcgcgc cctgctgccc gtagagggcc tggagcagat
     2401 tgccgcccat cgcgcgggcg tcaaaccggc gctgtggtgc gccggccccg cccgcctgac
     2461 ccgcgccctg gcgattgacg gcgccctcaa cggctgcgac ctgaccagcc cccctggtga
     2521 cctctggatt gaatctgctg cgctccccgc cacggcggag attgtcaccg gtccgcgcgt
     2581 gggcatcaac tacgcccccg aaccctggcg ctcgatggaa tggaatttta aacttgttga
     2641 ggtgcatcca tgacccccac ccccatcttc atttacggcg ctggtgggca cggcaagacg
     2701 cttgccgacc tgctgcgcgc cctgccagat tacgacc
//
LOCUS       DAOPVS010000219         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_38871_length_2721_cov_53.815079, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000219 DAOPVS010000000
VERSION     DAOPVS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38871_length_2721_cov_53.815079"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2721
                     /locus_tag="PKL11_10865"
     CDS             <1..>2721
                     /locus_tag="PKL11_10865"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNZ14143.1"
                     /translation="AHFLERLSKEISQHGTLHVLRKGIKDLGCKFQLAYFPPASGLNP
                     DIQRQYQGNVFSVVRQLKYSHTSENSLDLAIFLNGLPIFTAELKNPFNGQTVENAIRQ
                     YRTTRDHREPLFAYRRCLAHFAVDPELVFFATHLEGEKTRFIPFNQGYNLGAGNPPAW
                     RGFATAYLWEQVWARDSLLNLIQYFIHEYEEEDESGKKTGKRILIFPRYHQLDAVRRL
                     IADARQKGAGQHYLIQHSAGSGKSNSIAWLAHQLSVLHNAQNERVFDSVVVITDRRIL
                     DRQLQATVRQFEQTLGTVETITENSQQLKHALEAGKNIIVTTLQKFPVISEQIGSLPG
                     QHYAIIIDEAHSSQSGEATRHLSTVLAAGSLEEAEKQDTPAAEDDIEDRILTEIRRRG
                     QLPNASYFAFTATPKATTLELFGTRNTGGASAPFSLYPMRQAIEENFILDVLQNYTTF
                     KSYWSLLKKVQDDPAYDRSKATRLLMSFVDLHEHTIGKKAEVIVEHFVGQVQGRIGGH
                     AKAMIVTRSRLHAVRYKQAVDAYLKQHGYPFRALVAFSGTVKDGGIEYTESGMNGIPE
                     TQTAATFKLDEYRILIVAEKFQTGFDQPLLHTMYVDKRLKGLHAVQTLSRLNRVCPGK
                     AETFVLDFANDAEDIQKAFEPYYERTILSESTDPNLLYDLQTRLDQSGIYSTAEVDAF
                     AKIYYSNKATQAKLYAALAAVLERFKALDKDARAEFRNTLSDFVRLYAFLSQIATFAD
                     PDLEKLYVFGRLLLRRLPSDKDKLPLEVQQAIDLESWRLERTSSGKIDLQRGQGEIAP
                     EDPLNNSMRPNPELEALSQIIQELNQRFGTDFSSEDRLVIRQLEDHLAAIPMLEQTVR
                     VNTPENAMLTFREVLENQLNEMIDTHFKFYNQVNTNPDFAELLTRFLFDRF"
BASE COUNT          638 a          918 c          673 g          492 t
ORIGIN      
        1 gcccacttct tagagcgcct cagcaaagag atcagccagc acggcaccct gcatgtgctg
       61 cgcaagggca tcaaagacct gggctgcaaa ttccaactgg cttattttcc ccccgccagc
      121 ggcctcaacc cggacattca gcgccagtac cagggcaacg tcttcagcgt ggtgcgccag
      181 ctcaaataca gccacaccag cgaaaacagc ctggacctgg caatcttcct caacggcctg
      241 cccatcttta ccgccgaact caagaacccc ttcaacggcc aaacggttga aaacgccatc
      301 cggcagtacc gcaccacccg cgaccaccgt gaacccctct ttgcctaccg gcgctgcctg
      361 gcgcactttg ccgtagaccc cgagttggtt ttctttgcca cccatctgga aggcgaaaag
      421 acccgcttca tccccttcaa ccagggctat aacctgggcg ccggcaaccc gcccgcctgg
      481 cgcggctttg ccaccgccta cctgtgggag caggtctggg cgcgcgacag cctgctcaac
      541 ctgatccagt atttcatcca cgaatacgaa gaggaagacg aatccggcaa aaagaccggc
      601 aagcgcatcc tcatcttccc ccgctaccac cagttagatg ccgtccgccg cctgatcgcc
      661 gatgcccgtc agaagggcgc cggccagcat tacctcatcc agcactcggc cggcagcggc
      721 aaaagcaatt ccattgcctg gctggcgcac cagctttcgg tgctgcacaa cgcccagaac
      781 gagcgcgtct ttgattcggt ggtggtcatc accgaccggc gcattttgga ccggcagttg
      841 caggccacgg tgcgccagtt tgagcaaacc ctgggcactg tcgaaaccat caccgagaac
      901 tcacagcagc tcaaacacgc cctcgaagcg ggtaaaaaca tcatcgtcac caccctgcaa
      961 aaattcccgg tcatttcaga gcagatcggc agcctgccgg gccagcatta tgccatcatc
     1021 attgatgagg cccactcctc gcagtcgggc gaggccaccc gccacctcag caccgtgctg
     1081 gctgccggca gcctggaaga agccgaaaag caagataccc ccgcggctga agatgacatc
     1141 gaagaccgca tcctgactga aattcgccgc cgggggcagt tgcccaatgc cagctacttt
     1201 gcctttactg ccacccccaa agccacaacc ctggaactgt ttggcacccg caacacgggc
     1261 ggcgcctcgg cgcctttcag cctgtacccc atgcgccagg ccattgaaga aaatttcatc
     1321 ctcgacgtgc tgcagaacta caccaccttc aaatcttact ggagcctgct caaaaaggtc
     1381 caggacgacc ccgcctatga tcgcagcaaa gccacccgtc tgctgatgtc ttttgtggat
     1441 ttgcacgaac acaccattgg caaaaaagcc gaagtgattg tggagcattt tgtcgggcag
     1501 gtgcaggggc gcattggggg gcacgccaaa gcaatgatcg tcacccgttc ccggctgcac
     1561 gccgtgcgct acaaacaggc ggtagatgcc tacttgaagc agcatggcta tcccttccgg
     1621 gcgctggtgg ccttctcggg caccgtcaaa gatggcggga ttgaatatac cgagagcggc
     1681 atgaacggca tccccgaaac gcagaccgcc gccaccttca agctggatga ataccggatt
     1741 ttgattgtgg ccgaaaaatt ccagaccggc ttcgatcagc ccctgctgca caccatgtat
     1801 gtggataaac gcctcaaagg cctgcatgcg gtgcagaccc tctcgcggct caaccgggtt
     1861 tgccccggca aggccgaaac ctttgtgctc gactttgcca acgacgccga agatattcaa
     1921 aaagccttcg agccgtatta cgagcggacg atcctcagcg aatccaccga ccccaacctg
     1981 ctctacgacc tgcaaacccg tctggatcag agcggcatct attccaccgc cgaggtggac
     2041 gcctttgcca aaatctatta cagcaacaaa gccacccagg ccaaactcta tgccgccctg
     2101 gcagccgtgc tggagcgatt taaagccctg gataaagatg cccgcgccga atttcgcaac
     2161 accctttcag actttgtgcg gctgtatgcc ttcctgtcgc aaattgccac ctttgccgac
     2221 cccgacctgg aaaagctgta tgtctttggg cggctgctgc tgcgccggct gccatctgat
     2281 aaagacaagc tgcccttaga agttcagcag gccattgacc tggaatcgtg gcggctggaa
     2341 agaaccagca gcggcaaaat tgacttgcag cgcggtcagg gcgaaattgc ccccgaagac
     2401 ccgctcaaca acagcatgcg ccccaacccc gaactggagg cgctctcaca gatcatccag
     2461 gaactgaacc agcgtttcgg caccgacttc agctcagaag accggctggt catccgccaa
     2521 ttggaagacc acctggcggc catccccatg ctggagcaaa ccgtccgcgt caacaccccc
     2581 gaaaacgcca tgctgacctt tagagaagtg ttggaaaacc agctcaacga aatgatcgac
     2641 acccacttca aattctacaa ccaggtcaac accaaccccg acttcgccga actgctcacc
     2701 cgcttcctct tcgaccgctt c
//
LOCUS       DAOPVS010000220         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_39738_length_2679_cov_61.590701, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000220 DAOPVS010000000
VERSION     DAOPVS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39738_length_2679_cov_61.590701"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PKL11_10870"
     CDS             complement(<1..974)
                     /locus_tag="PKL11_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14144.1"
                     /translation="MSLARRGTWFNLYPLLWGAYPLLALAAVNLGQFDALALVRPLLL
                     SLLLAGLLLIVARRCTRDPHRAALLAIFWLSLFYTYGHVYTALEGSPLGHHRWLGLIW
                     LVLGALGTGLILRRLRNPQPASQFAAVLGVILLFLALGQMALFGLRQAQTRRATADQP
                     VMLKITPTSPDIYYIILDSYSRADMLQTYYGYDNSAFIAQLEALGFYVPPCAQSNYAL
                     TALSLSSTLNMDYLETYAADLADQNANWSHLGEYIQHSAVRTALEAQGYTTVSLESGV
                     PWNEWTDAGLYLSQTGSALANPNAREQISAFEQLYLRTTALRIATETG"
     gene            complement(971..2362)
                     /locus_tag="PKL11_10875"
     CDS             complement(971..2362)
                     /locus_tag="PKL11_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14145.1"
                     /translation="MAKSEARVAASFRDPAGFLFRREGLLYRQVNRAGEADYRLLMDS
                     GLYAALTKAGLLIPHTEVDVPPAEPDLAAVIIQPEAVGFISYPYEWSFSQLQDAALVT
                     LAIQRRALDRGLSLKDASAYNIQFQRGRPRLIDTLSFERYTEGTPWTAYRQFCQHFLA
                     PLALMAKRDIRLNQLLRIYIDGVPLDLASELLPGSTRLNLALLTHIHAHAGAQKRYAD
                     RGGEVKSSTAAASFSKNALLGLIDSLEAVVKSLRWKPAGTEWADYYDATNYTRAAFDA
                     KSERVASFLAQADPHIVWDLGANNGVFSRLAMARGAQTIAFDIDPAAVEQNYRQCRSA
                     KETLMLPLVMDLTNPSPALGWNHQERQSLAGRGPADLAMALALVHHLAIANNVPLPQL
                     AEFFAGLSEWLIIEFVPKGDSQVDRLLSSRVDIFPDYTPEGFEAAFASAFTITAKEPI
                     PDSRRILYLMKRR"
     gene            complement(2355..2579)
                     /locus_tag="PKL11_10880"
     CDS             complement(2355..2579)
                     /locus_tag="PKL11_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14146.1"
                     /translation="MTFFAPLAHGAAYLDPGSGSLLLQMLIALFLGAGFTISVYWKKI
                     KRFFKKGADEPAETIEIPEDTHETGGENNG"
BASE COUNT          539 a          763 c          900 g          477 t
ORIGIN      
        1 tgcccggttt cggtcgcgat gcgcagggcg gtggtgcgca ggtagagctg ctcaaaggcg
       61 ctgatttgct cccgggcgtt tggatttgcc agcgcgctgc cggtctgcga caggtagagc
      121 ccggcgtcgg tccactcgtt ccacggcacg ccgctctcca ggctgacggt ggtgtagccc
      181 tgcgcttcca gggcagtccg cacagcgctg tgctgaatat actcgcccag gtgagaccag
      241 ttggcgttct ggtctgccag gtcggcggcg taggtctcaa gatagtccat gttcagggtt
      301 gaagacagcg agagcgccgt gagcgcatag ttgctctgcg cacagggcgg cacatagaag
      361 cccagtgctt ccagttgagc gatgaaggcc gagttatcgt acccgtagta ggtttgcagc
      421 atatcggcgc gcgagtagct gtcgaggata atataataaa tatcgggcga ggttggggtg
      481 atcttcagca tgacgggctg gtcagcggtc gcgcggcggg tttgtgcctg ccgcaggcca
      541 aacaacgcca tttgccccag tgccaggaaa agcaaaatca cccccagcac agcggcaaac
      601 tggctggcag gttgaggatt gcgcagccgc cgcagaatca gcccggtgcc cagggccccc
      661 agcaccagcc agatgaggcc cagccagcgg tgatgcccca gcgggctgcc ctccagcgcc
      721 gtatagacat gcccgtaggt gtagaacagg ctcagccaga aaatcgccag cagggctgcc
      781 cggtgcggat cgcgggtgca gcggcgcgcc acaatcaaca gcaggccggc cagcagcagc
      841 gaaagcaaaa gagggcgcac cagcgccagc gcatcgaact gtcccagatt gacagccgcc
      901 agcgccagca gtggatacgc cccccacagc agcggatata aattaaacca ggtccccctg
      961 cgggcaaggc tcatcgccgc ttcatcaaat agaggatgcg gcgcgagtcg ggaatgggct
     1021 ctttggcagt gatggtgaag gcggaggcga aggccgcttc aaagccttcg ggggtgtagt
     1081 cggggaagat atctacgcgg ctggacagca aacggtccac ctgagagtcg cccttgggca
     1141 caaactcgat gatcaaccat tcgctcagtc cggcaaaaaa ttcggccagt tggggcagcg
     1201 gcacgttgtt ggcaatcgcc agatgatgca ccagcgccag cgccattgcc aggtcggcgg
     1261 ggccgcgccc ggccaaagac tgccgctcct ggtggttcca gcccagcgcc gggctggggt
     1321 tggtcaggtc catcaccagc ggcagcatca gggtctcttt ggcgctgcgg cactggcggt
     1381 agttttgctc caccgcggcg gggtcaatat caaaggcaat ggtttgcgcg ccgcgggcca
     1441 tggccagccg ggaaaagacg ccgttattgg cgcccaggtc ccagacgatg tgcgggtcgg
     1501 cctgcgccag gaaagacgcc acccgttcgc tcttggcgtc aaaggcggcg cgggtgtagt
     1561 tggtggcatc gtaataatcg gcccactcgg tgccggcggg cttccagcgc agcgacttga
     1621 ccacggcttc caggctgtcg atcagcccca gcagggcatt tttgctgaag ctggctgccg
     1681 ccgtagagga tttgacctcg ccgccgcggt cagcgtagcg tttttgagcg cctgcatggg
     1741 cgtgaatgtg cgtgagcagc gccaggttga ggcgggtgct gccgggcagc agttcgctgg
     1801 ccaggtcgag cggcacgcca tcaatgtaga tgcgcagcag ttggttcagg cggatatcgc
     1861 gtttggccat cagcgccagc ggagccagaa aatgctggca gaactggcgg taggccgtcc
     1921 agggggtgcc ttcggtgtaa cgctcaaaag agagcgtatc aatcaggcga gggcggccgc
     1981 gctggaactg gatgttgtag gcgctggcat ctttgagcga gaggccgcga tccagcgccc
     2041 ggcgttgaat cgccagggtg accagggcag cgtcttgaag ctggctgaag ctccactcat
     2101 acgggtaaga aataaagccc accgcctcgg gctggatgat taccgccgcc aggtcgggct
     2161 cggcgggggg cacatcgact tcggtgtgcg ggatgagcag cccggctttg gtcagggcgg
     2221 catagaggcc cgaatccatc agcaggcggt agtcggcctc gccggcgcgg ttcacctgac
     2281 ggtaaagcag cccttcacgg cgaaagagaa agccagccgg gtcacgaaac gaagcggcca
     2341 cacgcgcttc tgatttagcc attgttctcc ccgccggtct cgtgggtatc ttcgggaatt
     2401 tcgatggtct cggcgggttc gtcggcgccc ttcttgaaga accgtttgat cttcttccag
     2461 taaacgctga tggtaaaacc ggcgccgagg aagagggcaa tcaacatttg taagagcagg
     2521 ctgccggaac cggggtcaag gtaggcagcg ccatgggcaa ggggggcaaa aaaagtcatt
     2581 gagcgtactc ctgttacaga ttggcgacag gcttgatgac gatatcccaa taaagttctg
     2641 ccgcaccgaa ttgggggtat tataccaccg gattaaaag
//
LOCUS       DAOPVS010000221         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_40451_length_2645_cov_40.637452, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000221 DAOPVS010000000
VERSION     DAOPVS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40451_length_2645_cov_40.637452"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..1311)
                     /locus_tag="PKL11_10885"
     CDS             complement(472..1311)
                     /locus_tag="PKL11_10885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HNZ14147.1"
                     /translation="MVLKLSFILTVLIQIGVPLGLGFWLARRAANAGVFTRKAAWGIF
                     GVGVLTFIGSQVVHLPLLAGLTALFKLPGMWQPSAAFAPWFNAITLGLLAGLCEEPAR
                     WIGFKLLRAKARPRPAALLAGAGHGGIESIFIGLMVLVNFVTMLAVQAAINSGASLPA
                     ELELLKAQAGLFWDTPWHHVLAGGVERLAAITLHISLSVLVWRGVTAARGGGWWLAGA
                     ILLHGLVDMLAVASSQIWQWPVWSIEALVAALALGTAGLALWAWRTAPRPEPPLAAEP
                     VEP"
     gene            complement(1412..2170)
                     /locus_tag="PKL11_10890"
     CDS             complement(1412..2170)
                     /locus_tag="PKL11_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ14148.1"
                     /translation="MNFLTALRKEWLEQRRTRRLLIVIIVFVAFGLSSPLMAKFMPEV
                     MKSFPETAAIASMFPDPTAADAVGQYLKNMSQFGLLLAVLLSMGAVTNEKERGTAALM
                     LTKPLPRGSFLAAKAVALLITFGVGVALAGLGGYYYTYVLFEPLPVGGWLALNGLLLA
                     YIFVYVAITLLASTLARSQVAAGGIAFGLAILLGIFEAIPTTAKLMPAKLLAWGTQAA
                     LGSGETAWGALAVCLGLALAALLGAWLSLRKQEL"
     gene            complement(2167..>2645)
                     /locus_tag="PKL11_10895"
     CDS             complement(2167..>2645)
                     /locus_tag="PKL11_10895"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HNZ14149.1"
                     /translation="LDEPVSALDPAGRKDVLGLIEGLRGTNSALRGNCTVFMSTHILA
                     DVERICDSVAVIDHGRLLAQGRRDELIERYATPALRVEAAAGAAEAFAAWAAGLNALP
                     WVSAVTVDGLTATVQVTDLPLAQRELLRLAVAADLTLERYELVKPTLEDVFLRIVA"
BASE COUNT          515 a          898 c          812 g          420 t
ORIGIN      
        1 cgtctggtca ttgggtaaaa acagtatttt ggtcattcga taataataga acttatgttc
       61 tggacttatt atccggcatt ttgtgcgcga tgtcaaggtg taattcgttc cacgttccag
      121 gtacgaggag aggttccacg ttccaggttc caggggcgcg gaaggagtta agccaggggt
      181 gcggcgcatc cgactgttgt ggcgttgtag gggcggacac ataggtccgc ccctacaagc
      241 atacgtttta ccatcggcgg cggctaaagc ggactatgcc ggagggagtc ggcgcagccg
      301 acttgctctt taccagccgg ggatttcaat ccccggcgga ttttctggcg ctgcgcgagt
      361 tgcagttaga gcagggttaa ccaggtgcag gtatcttgca catttttgat gaaacatccc
      421 ccatctgatt aacaaaaaac cgggtgcgga ttgcccgcac ccggcccgtt atcagggttc
      481 aaccggctcg gccgccagcg gcggctcggg ccgcggcgcc gtgcgccagg cccacagggc
      541 cagccccgcc gtgcccagcg ccagggcagc caccagcgcc tcgatggacc agaccggcca
      601 ctgccagatc tgcgaggagg ccaccgccag catatccaca aggccgtgca gtaaaatggc
      661 ccccgccagc caccacccgc cgccccgcgc cgcagtcacg ccgcgccaga ccagcacact
      721 cagcgagatg tgcagggtga tcgccgccag gcgctccacg ccgcccgcca gcacatggtg
      781 ccagggcgta tcccagaaca gcccggcctg cgccttgagg agttccagct cggcgggcag
      841 gctggcgccg ctgttgatgg ccgcctgcac ggccagcatg gtgacaaaat tgaccagcac
      901 catcagcccg atgaagatgc tttcaatgcc gccgtgcccg gccccggcca gcagcgccgc
      961 cgggcgcggg cgggctttgg cccgcagcag cttgaagccg atccagcggg cgggctcttc
     1021 acacagcccg gccagcagcc ccagcgtgat ggcgttgaac cacggcgcaa aagccgccga
     1081 aggctgccac atgcccggca gcttgaacag cgccgtcagc ccggccagca gggggaggtg
     1141 caccacctgc gagccgatga acgtgagcac gcccacgcca aagatgcccc aggccgcctt
     1201 gcgggtgaag accccggcgt tggccgcccg ccgcgccagc cagaagccca gccccagcgg
     1261 cacgccgatt tgaatgagaa ccgtcagaat aaaacttaac ttgagaacca tgatcaactc
     1321 ctatgtttca cgtgaaacaa tttttgtgtt acttgcgcct gttagccggg gcgcaccgga
     1381 acctggaacg tggaacctgg cacgaacttt ctcacagttc ctgtttgcgc agggacagcc
     1441 aggcgcccag cagcgcggcc agggccagcc ccaggcagac ggccagggcg ccccaggctg
     1501 tttcgccgct gcccagcgcg gcctgcgtgc cccaggccag cagtttggcg ggcatcagtt
     1561 tggcggtggt cggaatggct tcaaaaatgc ccagcaggat cgccagccca aaggcaatgc
     1621 cgcccgccgc cacctgcgac cgggccagcg tgctggccag cagggtgatg gccacataga
     1681 cgaagatata ggccagcagc aggccgttca gcgccagcca gccgcccacg ggcagcggct
     1741 cgaacagcac gtaggtgtaa tagtagccgc ccagcccggc cagcgccacg cccacgccaa
     1801 aggtgatcag cagcgccacg gccttggccg ccagaaaact gccgcgcggc agcggtttgg
     1861 tcagcatcag cgccgcggtg ccgcgctctt tctcgttggt cacggcgccc atgctcaaca
     1921 gcaccgccag cagcagccca aactggctca tgttcttcag gtactggccc acggcatcgg
     1981 cggcggtggg gtcggggaac atggacgcaa ttgccgccgt ctccgggaag gatttcatca
     2041 cctctggcat aaactttgcc atcagcggcg aagacagccc aaacgccacg aagacgatga
     2101 tcacaatcaa caggcggcgg gtgcgccgct gttccagcca ttctttgcgt agtgcagtta
     2161 gaaagttcat gccacaatcc tcaaaaacac gtcttccagc gtcggtttga ccagctcgta
     2221 acgttccagc gtcaggtcgg cggctacagc cagccgcagc agctcgcgct gggccagcgg
     2281 caggtcggtc acctgcacgg tggcggtcag cccgtccacc gtcacagcgc tgacccaggg
     2341 cagggcgttc agcccggcag cccaggccgc aaaggcctca gccgcaccgg ctgccgcctc
     2401 cacccgcagg gcgggcgtgg cgtagcgctc aatcagctcg tcccggcggc cctgggccag
     2461 cagccgcccg tgatctatca ccgccacgct gtcgcaaatc cgctccacgt cggccaggat
     2521 atgggtggac atgaagaccg tgcagttgcc tctcagcgca ctgttagtgc cgcgcagccc
     2581 ttcaatcagc cccagcacat ccttgcgtcc ggcggggtcc agcgcgctga cgggttcatc
     2641 cagca
//
LOCUS       DAOPVS010000222         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_40452_length_2645_cov_33.540927, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000222 DAOPVS010000000
VERSION     DAOPVS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40452_length_2645_cov_33.540927"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..415)
                     /locus_tag="PKL11_10900"
     CDS             complement(92..415)
                     /locus_tag="PKL11_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14150.1"
                     /translation="MTNHSGASFWRRPGTRPGWWAVGLGLAYGVLSLINSAVFMRLSE
                     DLPWRQTVLPIYGIVMLLCGLAAGLVGAIAIIRQHERSWLVWLTVLAGALVVAFLLGE
                     FLIPH"
     gene            complement(561..1073)
                     /locus_tag="PKL11_10905"
     CDS             complement(561..1073)
                     /locus_tag="PKL11_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ14151.1"
                     /translation="MNIRLAETNDAPVLAGLVQGFRDALAALRDPYGVLRSSSPADLE
                     AARAELADYIEKRFPIYVAEDASGVLAGYLVCRVDGDVVWAESLYVRPEYRRQGVGSA
                     LYAAAEHLARSLGGDTPYNWVDPNNAAMIRFLQQRGYTVLNLIELRRPAPGESLPNRV
                     NVGAFEFEGH"
     gene            complement(1105..1662)
                     /locus_tag="PKL11_10910"
     CDS             complement(1105..1662)
                     /locus_tag="PKL11_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14152.1"
                     /translation="MKKRWILLGLVLFQTILTGCASKTPQLPDECLAQNMLISKEDIG
                     ALEEYALFSPMSDEPANSATISFQDPGLGSTFIIQHYDRNIESAYDNYSEFAFQTDEH
                     FGPWIRPGDLTFESTAADEFEYRCGRVWDEIHCIYVARYRNYLVDFGLIIGPEDFSLE
                     NFESGMAAIDREAAECFAALEEATP"
     gene            complement(1678..2262)
                     /locus_tag="PKL11_10915"
     CDS             complement(1678..2262)
                     /locus_tag="PKL11_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14153.1"
                     /translation="MKPYSEADFEKNISKVYFSVFANEDPYENPLQPGMRYRLLLYTF
                     SYGLNDYEPWLNPVVKTMKDLNEDGFYVTVLGPLNSIEEARHWYVPIEEAGTFMNTTY
                     PRENAIYSINGHWGIIGSDEEHGVAGGNAIFYNNLLSAVSDWDERLDMFLRAWKRFYL
                     EKRGNLDISWLPPLLTHIYGPVKTQSLLRKMDLD"
BASE COUNT          625 a          734 c          663 g          623 t
ORIGIN      
        1 aggctgtcaa aacctatcgg aaaattgcga tagctatcag gatgggtttt aggggttggg
       61 ttttgggata ttataaccgc ggatgagggc tctaatgcgg gatcaggaac tctcccagga
      121 gaaacgccac caccagcgca cctgccagca ccgtcaacca gaccagccag gaccgctcgt
      181 gctggcggat gatggcgatt gcgccgacaa ggccggcggc cagcccgcac agcagcatca
      241 cgatcccgta gatgggcagc acggtttgcc gccagggcag atcctctgac aggcgcatga
      301 acaccgcaga attgatgagg ctcagcaccc cgtaggcaag gcccaggcca actgcccacc
      361 agcccggccg ggtgcccgga cgccgccaaa acgaagctcc tgaatggttt gtcatttgga
      421 cctccagctt aattgcaccg ttgcctctga cctgaagcca ggcagcttga tccgcgtctg
      481 aattcattat acatcggcga gacttgaccg tttgaccagc atgcgttggg tttcgaagcc
      541 gctcacctgc cctgcgctca tcaatgcccc tcaaactcaa atgcgcccac attcacccgg
      601 ttgggcaggc tttcgccggg ggcgggtcgg cgcagttcga tcaggttcag cacggtgtag
      661 ccgcgctgct gcaaaaagcg gatcatggcg gcgttgttgg ggtccaccca gttatagggc
      721 gtatcaccgc ccagtgagcg ggccaggtgc tcggcggcgg catacagcgc cgagcccacc
      781 ccctgccggc ggtattccgg ccgcacatac agcgattctg cccagactac gtccccgtcc
      841 acccggcaga ccaggtagcc cgccagcacg ccggacgcat cctcggccac atagatcggg
      901 aagcgctttt cgatatagtc tgccagctcg gcgcgggcgg cttcaaggtc agccgggctg
      961 gagcttcgca gcaccccata agggtcgcgc agcgccgcca gcgcgtctct gaagccctgc
     1021 accaggccag ccagcacggg tgcatcgttg gtttctgcca ggcgaatatt catatcacca
     1081 tccttttcat caccgcgttg ggggtcaggg ggtggcctct tcgagggccg caaagcactc
     1141 ggctgcttcc cggtcaatgg cagccatgcc agattcaaag ttttccagac taaaatcctc
     1201 agggccaatt atgagcccaa aatcaaccag ataattccga taacgcgcta cgtatatgca
     1261 atggatttca tcccataccc gtccacatcg atattcgaac tcgtcagccg ctgtgctttc
     1321 aaatgttaga tcaccgggtc tgatccaagg cccaaagtgt tcatctgttt gaaatgcaaa
     1381 ttcagaataa ttatcatatg cagattcaat gtttctatca taatgttgta tgataaaggt
     1441 tgatccaaga cccggatctt gaaaactaat cgttgctgaa tttgcgggtt catcggacat
     1501 cggcgaaaat agcgcatatt cttctaatgc cccgatatct tctttggaaa ttagcatatt
     1561 ttgcgccaga cattcatctg gcagttgtgg cgttttggaa gcacaccccg tcaggatggt
     1621 ttgaaacaaa acaaggccaa gcaaaatcca tcgttttttc attttgggca ttttccttca
     1681 atcaagatcc atttttcgga gcaatgattg cgttttgaca ggcccataga tatgtgtcag
     1741 caaaggtggc aaccaactaa tatcaaggtt gcctctcttt tcaaggtaga aacgtttcca
     1801 ggctcgtaaa aacatatcta ggcgctcatc ccaatcggaa acggcgctga gtaaattgtt
     1861 gtaaaatatt gcgttccccc ctgccacgcc atgctcttcg tcagatccaa tgatccccca
     1921 gtgtccattg attgaataaa tcgcattctc tctaggatag gttgtattca tgaaagttcc
     1981 cgcctcttca atcggcacat accaatgacg ggcttcttcg atagagttaa gtgggcctag
     2041 cacagtcaca taaaaaccat cttcattcaa gtctttcatc gtttttacaa ccggatttag
     2101 ccacggttcg tagtcattta gcccatagct gaaagtatat aaaagcaaac gataacgcat
     2161 gccgggttga agtggattct cgtagggatc ctcgttggca aataccgaga agtaaacttt
     2221 gctgatattt ttctcaaaat cggcttcgga atatggtttc atggttgaat cttattctct
     2281 tcgcgtctca tggtcacatc agcccatgcc agtcaaacca cccgtgaaca ggatgccttg
     2341 attctagact cggcggtgag atttgtcaaa tagcgtttaa cctttcacgt ttcgggattc
     2401 tggtgcgacg acccgaatgg gtcgcgcagc atccttgcgg ggcgctacac agacgcaaat
     2461 aaacgtaagg gtgctgcgcg actttgcgtc ttggcgttgg cttcttaaac tgcaactcag
     2521 ggcgcacagc ctgttcaggc tgcccgtcca caggtgcgcc cctacagaag aaccgccgtc
     2581 attcagcgct ctaaaattct agaacacctg cacagtgggg cggaagttca gttcgtcaat
     2641 caggg
//
LOCUS       DAOPVS010000223         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_40547_length_2641_cov_58.908353, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000223 DAOPVS010000000
VERSION     DAOPVS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40547_length_2641_cov_58.908353"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PKL11_10920"
     CDS             <1..654
                     /locus_tag="PKL11_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNZ14154.1"
                     /translation="PLAGRKVVVTAGGTQEALDPVRVLTNRSSGKQGYALAQAAIDLG
                     AEVTLITAPTVLAPPIGATVRRVVNAAEMLTTVLQETEAADALIMAAAVADFRPKSIA
                     VQKMKKSGGLPHVELEPTDDILAAVARRRAETGFPHRTVGFAAETEALLTNANVKLAA
                     KRLDMIAANDVSAPDAGFEVDTNRVVLLFADGRQESLEKRSKAEIAEIIMQHVVEWF"
     gene            654..1883
                     /locus_tag="PKL11_10925"
     CDS             654..1883
                     /locus_tag="PKL11_10925"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNZ14155.1"
                     /translation="MRRWWVLGWLLLLCLVGCSGLSVLVEPLPTAAVLMPSPIPPTPV
                     WSATPLPTVIPTPTASITPTLMPVGTLPAGYEATPRPNVIGVLPFPADVNPLTGLRVS
                     NPEVLEHRPLLIKISNYPRRGRPHAGLGQADFVFDYYIGEGLDRFLAVFYSQQPTNIG
                     PLRSGRLVDAQLTDFYGGVLVYRGADPVVDDDIVATLPARSIADAPCPAICDGYDAYK
                     TSTFVSVPGLRDWMAAKGWDDSRQDLNGMVFSEAAPQSSLTADMLGVQFSNINRTEWY
                     YDPTTGKYARYYESDVTEDEFVMVQSIDRNTGNQVEFDNLIILFAEYIEYKETLHNVY
                     MNTNFAGQPALFFRDGRAVEGTWRITGKQDPIIFTDANGIAYPLKPGVTWVYILGLAS
                     TVEQVEPGHWEADFDLP"
     gene            1896..2621
                     /locus_tag="PKL11_10930"
     CDS             1896..2621
                     /locus_tag="PKL11_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ14156.1"
                     /translation="MRILVMSDIHANLSALEAVLNDAGTVDAVWCLGDLVGYGPNPNE
                     CVQRIQELPNLVCVQGNHDAAALGKIDTEAFNNEAGQALRWTKTALSTSSLAFLDSLP
                     EKQVVDLVTLTHGSPRRPIWEYILDTYTAALNLLALETPYGFVGHSHHPVVFMVNGED
                     AVTYELPEADQVVEMPPLAIFNPGSVGQPRDHDPRAAYAIYEPEIRRWTQRRIAYDIV
                     ETQNRIRAAELPLRFAVRLVEGS"
BASE COUNT          548 a          659 c          870 g          564 t
ORIGIN      
        1 ccattggccg ggcgcaaggt ggtggttacg gcggggggaa cccaggaggc gctggacccg
       61 gtgcgggtgc tgaccaaccg gtcatcgggc aagcagggct atgcgctggc acaggcagcc
      121 attgacctgg gcgccgaggt tacattgatt actgccccaa ccgttttggc acccccgata
      181 ggagccaccg tgcggcgggt ggtcaatgcg gctgagatgt tgaccacggt tttgcaggaa
      241 accgaggcgg cagatgcctt gattatggca gcagccgtgg ccgattttcg gcccaagtcg
      301 attgctgtac agaagatgaa aaagagcggc ggactgccgc atgtggagtt ggaaccaaca
      361 gatgatattc tggcggcggt ggcccggcgc cgcgctgaga ccgggtttcc gcaccgaacg
      421 gttggatttg cggcggagac tgaagctctg ttgaccaatg ccaacgtcaa gctggcggcg
      481 aaacggctgg atatgattgc ggccaatgac gtcagcgcac cggatgccgg gtttgaagta
      541 gataccaacc gggtggtgct gctgtttgcc gatgggcggc aggaaagtct ggagaagcgc
      601 agcaaggcag agatcgctga aattattatg caacacgtgg tcgaatggtt ttgatgcgcc
      661 gctggtgggt gctgggctgg ctgctgctgt tgtgtctggt gggatgcagc ggattgagcg
      721 tgctggttga gccgctgcca acagcggcag tcttgatgcc atctccgatc ccccctaccc
      781 cggtgtggtc tgccacgccg ctgcccacgg tgatcccaac gcctacagcc agcatcacac
      841 ccacgctgat gcccgtgggg acgctgccgg ccgggtacga ggcaacgccc cggcccaatg
      901 tgatcggcgt gctgccgttt ccggcagatg tgaatccact gaccgggtta agggtaagca
      961 atccggaggt gctggagcac cgtccgctgt taatcaaaat atcgaattat ccccggcgcg
     1021 ggcggccaca tgcagggctg ggacaggccg attttgtttt tgattattat attggcgagg
     1081 gactggatcg ttttctggcg gtcttttaca gccagcagcc caccaatatt gggccgctgc
     1141 gctcggggcg gctggtggat gcgcagttga ctgattttta cggcggagtg ctggtgtacc
     1201 ggggggcaga cccggtggtg gatgatgata ttgttgccac gctgccggcg cgctcgattg
     1261 ccgatgctcc ctgcccggca atttgcgatg gttatgatgc ctataagacc agcacatttg
     1321 tcagtgtgcc gggcctgcga gactggatgg cggccaaggg ctgggatgat tcacggcaag
     1381 acctgaatgg gatggttttt tcagaggccg caccgcagag cagcctgacg gctgatatgc
     1441 tgggggtgca gttcagcaat atcaaccgga cagagtggta ttatgacccc acaaccggga
     1501 aatatgcccg atattacgaa agtgatgtga cggaagatga atttgtgatg gtacaatcca
     1561 ttgaccgtaa cacgggcaat caggtagaat ttgataatct gatcatcctg ttcgccgaat
     1621 acatcgagta caaagaaacc ttgcacaacg tttatatgaa caccaatttt gccggacagc
     1681 cagccctgtt cttccgagac gggcgggcgg tagaaggcac ctggcgcatc actggcaagc
     1741 aggacccgat tatctttacc gatgccaatg gcatcgctta tccattaaaa cccggggtaa
     1801 cctgggtgta tatcctgggg ctggcatcga ccgttgaaca ggttgaaccg gggcactggg
     1861 aagctgattt tgatctgccc tgaggggaga cctacatgcg cattctggta atgtctgata
     1921 ttcatgcgaa cctttcggcg ctggaagcgg tgctgaacga tgccgggact gttgatgcag
     1981 tgtggtgtct gggtgatctg gtgggatatg ggccgaatcc gaatgagtgc gtgcagcgca
     2041 ttcaagagtt gcccaacctg gtttgcgtcc aggggaatca cgacgcggct gcgctgggga
     2101 agattgatac tgaggcattt aataatgaag ccggacaggc gctgcgctgg acaaagacag
     2161 cgctgagtac cagctcgctg gcgtttttgg acagcctgcc cgaaaagcag gtggtggatc
     2221 tggtgacgct gacacacggc agcccgcgcc gccccatctg ggagtatatt ctggatactt
     2281 ataccgcggc gctcaatttg ctggcgctgg aaacgccgta tgggtttgtg ggccattcac
     2341 accatccggt ggtgttcatg gtgaacggcg aggatgcggt gacatacgaa ctgccggagg
     2401 cagaccaggt ggttgaaatg ccgccactgg cgattttcaa tccggggtcg gtggggcagc
     2461 ccagagatca cgacccgcgg gcagcttatg ccatttatga gcctgagatt cggcggtgga
     2521 cgcaacggcg gattgcctat gatattgtgg aaacgcaaaa ccgcattcga gcggcggaac
     2581 tgccgctgcg gtttgccgtg cgactggtgg aggggagttg aggatttagg aatgaccaat
     2641 g
//
LOCUS       DAOPVS010000224         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_40979_length_2620_cov_44.109162, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000224 DAOPVS010000000
VERSION     DAOPVS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40979_length_2620_cov_44.109162"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PKL11_10935"
     CDS             <1..1236
                     /locus_tag="PKL11_10935"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14157.1"
                     /translation="FSVNADKLALAQQYLISALAAPDMMMADWELDRLVFQYYVLSEM
                     GAPDLDINAFFALRDRLSPSAQALTALMLSTNGNSEQARTLLSDLEANALRSATGVHW
                     QDHDTGWYNFSTPEYATAAVVFALARLDPASPLVNDAVRYLAAHRRPHGWGSIYQNAW
                     VLTALTDVMIANGEALAEYDYTVALNDVPLAEGSVSGADLGSSTTATAPLDQLDPVNP
                     NALLFSHGEGNGRLYYRAYLQVYQAVEDAEPVNRGISISRSYIMAAEDCRLPECTRIN
                     GVTLNDTESVIRVVLDVTVPEDMYYVAIEDFIPAGAEVVNVGLKTTQMGGDDPNQLYD
                     PADPYRAGWGWWYFDDPQISDSGIRWMAPYLPAGSYTVTYRFLPTQAGEYRVLPARAW
                     MYFFPEVEGRTGGDIFDIR"
     gene            1530..2039
                     /locus_tag="PKL11_10940"
     CDS             1530..2039
                     /locus_tag="PKL11_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14158.1"
                     /translation="MSEEDQMNQQPDTVGVVYQQLCKSHRAIGDFRARLLVCLPLATA
                     GALILMEPLSDKTRLLLGIFGFLVSLGLYFYEFLANLRCEHLIKVGKQLEAQLAINGV
                     YTERPTGLVGFVNEAYLARVVYPVVLAAWAYSAFSPTGPVLSVIMALAVLALFFFIPR
                     LLKLGQWPI"
     gene            2064..2510
                     /locus_tag="PKL11_10945"
     CDS             2064..2510
                     /locus_tag="PKL11_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ14159.1"
                     /translation="MKHIRNSVKAIIIQNNSLLCIRKIDELGFYYLLPGGGQDKNETF
                     IEAVERECLEELGAAVHVGQLRYIREYIGEHHEFRETDHNHQIEFMFECTLCAEPDLQ
                     KASHCDEGQDGIEWVSLDSQTHRVYPKVLLERIRQGYAEVYWGDIN"
BASE COUNT          545 a          728 c          789 g          558 t
ORIGIN      
        1 ttctcggtca atgccgacaa gctggcgctg gcccagcagt acctgatcag tgcgctggcg
       61 gcgccggata tgatgatggc ggattgggaa ctggaccgcc tggtcttcca gtactatgtg
      121 ctgagcgaaa tgggtgcccc cgacctggac atcaacgcgt tctttgcact gcgtgaccgg
      181 ctgagcccct cggcccaggc actgactgcc ctgatgcttt caaccaacgg aaacagcgaa
      241 caggcccgca cactgctctc tgacctggag gccaacgccc tgcgctcggc aaccggggtg
      301 cactggcagg atcatgatac gggctggtac aacttctcca cgccggaata tgccaccgca
      361 gccgtagtct ttgcactggc ccggctggac ccggcctcgc cgctggtgaa tgatgccgtg
      421 cgctacctgg cggctcaccg gcgcccgcac ggctgggggt cgatctatca aaacgcctgg
      481 gtgctgaccg ccctgaccga tgtgatgatt gccaacggcg aagcgctggc ggagtatgat
      541 tacaccgtgg cgctgaatga tgtgccgctg gctgaaggca gcgtcagtgg ggcagacctg
      601 ggcagcagca ccacggccac cgcgccgttg gatcagcttg acccggtaaa ccccaatgcg
      661 ctgctgttct cgcacggcga gggcaatgga cggttatatt accgtgcgta tctgcaggtc
      721 tatcaggcgg tggaagatgc cgaaccggtc aaccggggca tcagcatctc ccgcagctac
      781 atcatggcgg cggaagactg ccgcctgccg gaatgcaccc gcatcaacgg cgtgacgctg
      841 aacgacaccg agtcggtgat tcgcgtggtg ctggatgtga cggtgccaga ggatatgtat
      901 tacgttgcga tcgaagactt catccccgcg ggtgcggagg tagtgaatgt aggtctgaag
      961 accacacaaa tgggtggcga cgatccgaat cagctctatg acccggcaga tccctaccgc
     1021 gcgggctggg gctggtggta cttcgatgat ccgcagatca gcgactcggg catccgctgg
     1081 atggcgcctt acctgcccgc cggcagttac acggtcactt accgcttcct gcccacccag
     1141 gcaggcgagt accgcgtgct gcccgcccgg gcgtggatgt acttcttccc cgaggttgag
     1201 gggcgcaccg gcggcgatat cttcgatatc cggtaatctg ggttaacttg atccgggcga
     1261 ggcgtaccgc agattgggcg ggtgcctcgc ccgggattgt tttgctgttc gggcagttgc
     1321 agaggcaaag ccttctacaa tcaccgagaa atgaaatcct ttaccctgtg aagtcatcaa
     1381 aatacggggt gggccgccgg ggattaaaat ccccggctga taaaagggca agtcggctgc
     1441 gccgactccg gtggtagaat aaaggaagct attaaactac ccaaacaagc actgcctgtg
     1501 cactgtttga tggttgaaga aggtcaacca tgtctgaaga agatcaaatg aaccaacagc
     1561 ccgacacggt cggggttgtg taccagcagc tttgcaagag tcatcgagcc attggtgatt
     1621 ttagagccag gcttctggtc tgtctgccgc tggctaccgc aggcgccctc atattgatgg
     1681 aaccgctcag cgacaaaacc cggctgctgc tgggaatttt cgggtttttg gtcagcctcg
     1741 ggctgtattt ctacgagttt cttgccaatc tgcgctgtga gcatctgatc aaagtgggca
     1801 agcagttgga agcgcagctt gccatcaacg gggtgtacac cgagcgcccc actgggctgg
     1861 tggggtttgt caatgaggct tacctcgccc gcgtcgttta cccggttgtg ctggctgcct
     1921 gggcctacag cgcgtttagc cccaccggac cggtgctgag tgtcatcatg gcactggctg
     1981 tgctggcgct tttctttttc atcccacgcc tgttaaagct cgggcagtgg ccgatttaag
     2041 cgagatagcc taagattgac actatgaaac acatcagaaa ctccgttaaa gccatcatca
     2101 tccaaaacaa cagcctgctg tgcattcgca agattgatga attgggcttt tattacctcc
     2161 tgccgggcgg cgggcaggat aaaaacgaga cgttcatcga agccgttgaa cgcgaatgcc
     2221 tggaagaact gggcgcggcg gttcatgtgg gccagctcag gtatattcgc gagtacattg
     2281 gggaacatca cgagtttcgt gaaaccgacc acaaccacca gatcgagttt atgtttgagt
     2341 gcacgttgtg cgccgaaccc gaccttcaaa aagccagcca ctgcgatgaa gggcaggatg
     2401 ggattgagtg ggtcagtctg gacagtcaaa cccaccgggt gtaccccaaa gtgctgctgg
     2461 agcgcatcag gcaggggtat gcggaagtgt attggggcga tatcaattag ggccttatga
     2521 acgcagcctg ccagcctgtc aacttggcgg atattcagtg cattgttttt gatttcggct
     2581 ttacgctctc atcagatttg tatttcaagg cagccccgcc
//
LOCUS       DAOPVS010000225         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_41025_length_2618_cov_57.231760, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000225 DAOPVS010000000
VERSION     DAOPVS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41025_length_2618_cov_57.231760"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PKL11_10950"
     CDS             <1..1215
                     /locus_tag="PKL11_10950"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ14160.1"
                     /translation="QAFSLIGSALVQFALVWWMTSQTGSATVLATATLFALLPQIILG
                     PFVGALVDRWNRRLIMILADSGIALATGVLIYLFATEQIQVWHVYVILLIRSLGGAFH
                     GPAMTASTSLMVPPQHLARLAGLNQTLQGLISIFAPPLGAMLIGLLPLEGVLAIDIGT
                     AALAVLPLLFIPIPRPPRQTAQDNGTAAKTSYWQDLAQGFRYVVKWPGLLGLILMAML
                     LNFLLVPSSSFMPLVVTQIFNKGVVELGWTETLFGVGMIVGGLTLGAWGGFKRKIITS
                     LVGIIGISLGVILIGVAPGNMFFLLLVANFILGATQVFANGPLGAIFQSTIEHDMQGR
                     VFSLINAGATAMMPLSLMVAGPMSDWLGMRIWYLIGGGLCILVTIIAFFTPAIVTIEE
                     QRKAVAVPQEEI"
     gene            1215..1982
                     /locus_tag="PKL11_10955"
     CDS             1215..1982
                     /locus_tag="PKL11_10955"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNZ14161.1"
                     /translation="MFKNAIVRTPCPAITEGITTANLGKPDYAAALEQHAAYIEALKA
                     CGVAVTILEADPAYPDSCFVEDTAVLAERVAVITNPGALTRKGEEKKVEAALRQFYTP
                     EQIKHISAPGTLEGGDVMRVGDHFYVGLSARTNAEGARQFFEHLNAYGYTGSVVQMSE
                     MLHLKTGLAYLENNLLLTAGEFVGHPVFASFNKIEIDLADAYSANCIYVNGTVLIPAG
                     YPATRAKIEAAGLKTLAVDTSEFRKIDGGLSCLSLRF"
     gene            complement(1979..>2618)
                     /locus_tag="PKL11_10960"
     CDS             complement(1979..>2618)
                     /locus_tag="PKL11_10960"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ14162.1"
                     /translation="TEVPQHRVNLSEYWMGRTPVTNAQYAVFTLSTGAKAPEHWRSNQ
                     PPQALLDHPVVFVSWYEAAAYCEWLSQQSGRRVSLPTEAQWEKAARGTDGRIYPWGDE
                     APDAARCNFNRSNQTTPVGQYSPLGDSPYGCVDLVGNVLEWCADWYEDNYYLCSPADN
                     PPGPASGERRVLRGSSWFDSAPFVRAARRACTRPDFSLYLWGFRCVLVNQPT"
BASE COUNT          494 a          744 c          824 g          556 t
ORIGIN      
        1 caggcttttt cgctgattgg ttcagcgctg gtgcagtttg ccctggtgtg gtggatgacc
       61 agccagacag gctcggctac cgtgctggca accgccacgc tgtttgccct gctgccgcag
      121 atcatcctgg ggccttttgt gggcgcgctg gtggaccgct ggaaccggcg gctgatcatg
      181 attctggctg acagcggcat tgccctggcc acgggcgtgt tgatctacct gtttgccacg
      241 gagcagattc aggtctggca tgtttatgtc atcctgctca tccggtcgct gggcggagct
      301 tttcacggcc cagctatgac ggcctccacc tcgttgatgg tgccgccgca gcacctggca
      361 cgcctggcgg gtttgaacca aaccctgcag gggctgatca gcatctttgc gccgccgctg
      421 ggtgccatgc tgattggcct gctgccgctg gaaggggtgc tggcgattga tatcggcact
      481 gcggctctgg ctgtactgcc gctgctgttc atcccgatcc cgcggccgcc ccgccagacc
      541 gcgcaggaca acggcacagc cgctaaaacc agctactggc aggacctggc tcagggcttt
      601 cggtatgtgg tcaaatggcc ggggctgctg ggattgattt tgatggccat gctgctcaac
      661 ttcctgctcg tgccttccag ctcctttatg ccgctggtgg tgacccagat cttcaacaag
      721 ggcgtggtgg aattgggttg gactgaaacc ctgtttgggg tgggcatgat tgtgggcggc
      781 ctgacgctgg gcgcctgggg tgggttcaag cggaagatta ttacatcact ggtcgggatc
      841 attggcatca gcctgggcgt gattttgatt ggcgtggcac cggggaatat gttcttcctg
      901 ctgctggtgg ccaactttat cctgggagcg acccaggtat ttgccaatgg gccgctgggg
      961 gccatcttcc agagcacaat tgaacatgac atgcaggggc gggtcttttc gctgatcaat
     1021 gcgggggcaa ccgcgatgat gcccctgagc ctgatggtgg ctggccccat gtcggactgg
     1081 ctggggatgc gcatctggta tttgattggc ggcgggctgt gtattctggt gacgataatt
     1141 gccttcttta cgccggcaat cgtcacgatt gaagagcagc gcaaagccgt ggcagtgccg
     1201 caggaggaaa tttgatgttc aaaaacgcaa ttgttcggac accctgcccg gccatcaccg
     1261 agggcatcac aacggccaac ctgggcaagc ccgactatgc cgccgccctg gaacaacatg
     1321 ccgcttacat tgaagctttg aaggcttgcg gtgtggcagt gactattctg gaggcagacc
     1381 cggcctaccc cgactcttgt tttgtggaag atacggcggt gctggctgag cgcgtagccg
     1441 tgatcaccaa cccgggcgca ctcacgcgca agggcgagga aaagaaggtc gaagcggcgc
     1501 tgcggcagtt ttacacccct gagcagatca agcacatctc ggcgccgggc accctggagg
     1561 gcggcgatgt gatgcgcgtg ggggatcact tttatgtggg cttatcggcg cgcaccaatg
     1621 cagaaggcgc ccggcagttc tttgaacacc tgaatgccta cggttacacc ggttcagtgg
     1681 ttcaaatgag tgaaatgctg cacctgaaaa ccgggctggc ttacctggaa aacaatctgc
     1741 tgctgacagc cggggagttt gtggggcacc cggtttttgc cagcttcaac aaaattgaaa
     1801 ttgacctggc ggatgcctat tcagcaaact gcatctacgt caacggcacg gtgttgatcc
     1861 cggcaggtta cccggccacc cgagccaaga tagaagctgc gggcctcaaa acgctggcgg
     1921 tggatacatc agaatttcgc aaaatagatg gcgggctgag ctgcctgtca ctgcggttct
     1981 aggtcggttg attgacaagc acacaacgaa agccccacag gtagagcgag aagtcggggc
     2041 gggtacaagc gcgccgcgcc gcacgcacaa acggcgctga atcgaaccag gagctgccgc
     2101 gcaggacgcg ccgctccccg gaggcgggcc cggggggatt atccgcaggg gagcaaaggt
     2161 aatagttatc ctcgtaccaa tccgcgcacc attcgaggac atttccgacc agatccacgc
     2221 agccgtaagg gctgtcgccc agagggctgt actgccccac cggggtggtc tgattgcttc
     2281 gattgaaatt gcagcgagcc gcatccggcg cctcatcgcc ccacggatag atacgtccat
     2341 cagttccgcg ggcggctttt tcccactgcg cctcggtggg caagctgacc cttctgccgg
     2401 actgttgact cagccattca caataggcag cagcctcata ccagctcaca aataccaccg
     2461 gatgatccag cagggcttgc gggggctggt tactgcgcca gtgttctggc gctttggcgc
     2521 ccgtggacag ggtgaatacg gcatactgcg cattggtcac cggggtgcgc cccatccaat
     2581 actctgacag gttcacacgg tgctgcggca cctcggta
//
LOCUS       DAOPVS010000226         2591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_41675_length_2591_cov_64.318612, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000226 DAOPVS010000000
VERSION     DAOPVS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41675_length_2591_cov_64.318612"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..613)
                     /locus_tag="PKL11_10965"
     CDS             complement(167..613)
                     /locus_tag="PKL11_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNZ14163.1"
                     /translation="MLVRYDPFREAFAMRRAMNRALQSVMDTPADVDEPQMSWSLALD
                     VVEQDEAFLIKATVPGINPDDLEITFQDNLLTIRGEITEDKDLTEGKLHLRERRCGAF
                     SRTVKLPGQITADKIEARYEAGVLTLRLPKAEEMKPRKIAVQVAKK"
     gene            851..1885
                     /locus_tag="PKL11_10970"
     CDS             851..1885
                     /locus_tag="PKL11_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNZ14164.1"
                     /translation="MELKQLHEIPLFRNLTEGDLREVSLLLEEVEIEPGAVFMHEGDA
                     GDKLYIIVDGQVEIVKALGTPQELPLLHCQPGDFIGEMSLINPDGLRTASACCNGPAR
                     LLVLHQKDFNRLMETRPAVAMVILRELSTRLRETSNAVIHRLQQANDELTHAYDMTLE
                     SWALALALRDNETESHTRRVADLTTHLAAELGVRGEDLTHIRRGAILHDIGKIFVPDS
                     ILLKPGPLTDEEWKVMRMHPVYAQQILAPVAFLQKSVEVPYNHHERWDGTGYPRGVKG
                     EAIPLGARIFAVIDVWDALVSDRPYRKALPEEMVLDYLKQQSASHFDPQILQAFLDLH
                     QSGTLHEHTA"
     gene            1889..>2591
                     /locus_tag="PKL11_10975"
     CDS             1889..>2591
                     /locus_tag="PKL11_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNZ14165.1"
                     /translation="MHIRVGLENEFEGRSLAWALDYPGCFAYGPDASTALVRVVPALI
                     AYQDWVGRHTTSSWLTNLGDFDVRLSEVWQVYRLNEDYETIPQGGYEVNAWFQSDWLP
                     LTRAEVRRAAQLLEWTRADLLSVTADLTDAQLDAERPGQRWSIRGILAHVATAEWWYL
                     DRLGLAGGERAALPKDPLARLAAVRTRLLAALPALEGLEQVAGKQGELWSPRKLVRRA
                     LWHERDHVNHILELKL"
BASE COUNT          504 a          763 c          812 g          512 t
ORIGIN      
        1 ctaaagcgga ctcagccaaa cgcagtcggc gcagccgact tgctctttgc atgcggctct
       61 ttacggggtg ctgtgcggcc cttctgacgg caaaacaaac ccagcccgca ctctgggagg
      121 ggagatgcgg gctgggggtt gggtgccgct tgtgcggcga gaagggttat ttcttggcga
      181 cctgcacggc gatcttgcgg ggcttcatct cttcggcttt gggcagccgc agggtcagca
      241 caccggcctc gtagcgggct tcgatcttgt cagcggtaat ctgccccggc agcttgacgg
      301 tgcggctgaa agcaccgcag cgccgctcac gcagatgcag cttgccctcg gtcaggtctt
      361 tgtcttcggt aatttcaccg cgaatggtca gcagattatc ctggaaggtg atctccagat
      421 catcggggtt gatgcccggc acagtggcct tgatcaggaa cgcttcgtcc tgctcaacca
      481 cgtcgagagc cagactccag ctcatctgcg gttcatcaac atctgcgggg gtgtccatca
      541 cggactgcag cgcccggttc atggcccggc gcatggcaaa cgcttcacgg aaaggatcgt
      601 atcgaactaa catggcagcc tcctgcttgt gtctaatgtt tggttatttc tgcggtcagc
      661 acgtctcttt ctgctgacag ccttatgata gcccgggcct gtaagaatca aatcaacaaa
      721 atattggaaa aatatcagag ttgtaagctt aaaaaacttg tcattcgcgg cgcttcatgc
      781 taggatgaag gcataaatgc agcagcgggt aagcagctgt ttatgcattg atttaacgga
      841 gtataaaagt atggaactca aacaactgca cgagatccca ttatttcgta atttaaccga
      901 aggcgatctg cgcgaagtat cgctgttatt ggaagaggtt gaaattgagc cgggggcggt
      961 cttcatgcac gaaggcgacg ccggcgacaa actctatatc attgtggacg ggcaggtaga
     1021 gattgtgaag gcgctgggca ccccccagga gctgccgctg ctgcactgcc agcccggtga
     1081 ttttatcggc gagatgtcgc tcatcaaccc cgatgggctg cgcactgcca gcgcctgctg
     1141 caacggcccc gcccgcctgc tggtgctgca tcaaaaagat tttaaccgcc tgatggaaac
     1201 ccgccccgcc gtggccatgg tcattctgcg ggagctgtcc acccggctgc gtgagaccag
     1261 caacgccgtg atccaccgct tgcagcaggc caacgacgaa ctgacccacg cctacgatat
     1321 gacgctggaa agctgggcgc tggccctggc gctgcgcgat aacgagaccg aaagccacac
     1381 ccgccgggtg gccgacctga ccacccacct ggctgcggaa ctgggcgtgc gcggcgaaga
     1441 cctgacgcac atccgccgcg gcgccatctt gcatgatatt ggcaaaatct tcgtccccga
     1501 ttccatcctg ctcaagccgg ggccgctgac cgatgaagaa tggaaggtga tgcgcatgca
     1561 cccggtctat gcccagcaga ttctggcgcc ggtggccttc ctgcaaaaat cggtggaggt
     1621 gccctacaac caccacgagc gctgggatgg caccggctac ccgcgcggcg tcaagggcga
     1681 ggccatcccg ctgggggcgc gcatttttgc tgtgattgat gtgtgggatg cgctggtcag
     1741 tgaccgcccc taccgcaagg ctctgcccga agaaatggtg ctggattacc tgaagcagca
     1801 gagcgcctcg cactttgacc cgcagatttt gcaggccttt ttagacctgc atcagagcgg
     1861 cacgctgcac gaacatactg cataaaccat gcacatccgc gtggggctgg aaaacgagtt
     1921 tgaaggccgc tcgctggcct gggcgctgga ttatcccggc tgttttgcct acggccccga
     1981 tgccagcaca gccctggtgc gggtggtgcc cgccctgatc gcctaccagg actgggtggg
     2041 gcggcatacg accagctcgt ggctgaccaa cctgggcgat tttgacgtgc gcctgagcga
     2101 ggtctggcag gtctatcgcc tgaacgaaga ttatgagacg attccgcagg gcggctatga
     2161 ggtgaatgcc tggttccaat cagactggct gccgctgacc cgcgccgagg tgcgccgcgc
     2221 cgcccaactg ctcgagtgga cccgcgccga cctgctgtcg gtcacggccg acctgaccga
     2281 cgcccaactg gacgccgaac gtcccggtca gcgttggagc attcgcggca ttctggcgca
     2341 tgtggctacg gctgaatggt ggtacctgga ccggctgggg ctggcggggg gtgagcgtgc
     2401 cgccctgccc aaagatccac tggcgcggct ggcggcggtg cggacgcggc tgctggcagc
     2461 cctgcccgcg ctggaaggcc tggagcaggt ggccggcaaa cagggcgaac tgtggagccc
     2521 gcgcaagctg gtgcgccggg cgctgtggca cgagcgcgac catgtgaatc atattttgga
     2581 gttgaagctg g
//
LOCUS       DAOPVS010000227         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_41810_length_2586_cov_52.963651, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000227 DAOPVS010000000
VERSION     DAOPVS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41810_length_2586_cov_52.963651"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..2453)
                     /locus_tag="PKL11_10980"
     CDS             complement(150..2453)
                     /locus_tag="PKL11_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNZ14166.1"
                     /translation="MSDPNNKRRAALYGRVSTEMQAGEDHFSIEAQLAEMHEYCERKG
                     YQVVGEFVDRGISGSQRSRPQLDNLMALASNRQFDVLIVHDLSRLSRTVYHTLDILQD
                     LGKWNITFASVKDPDFDFTDPAKQFFLIILAAMNQYFLTQLRLHTAKSKNQRARVGLY
                     NASVPPYGYARGEDPRQPFTINEEEARAVRFIYEQYATGRYSHQQIADLLAAAGFRPR
                     PSPRRRGETPTVRQQFSKETVTDILKNPFYTGRVGYRIKHGKAKEIYEGRHEPLISLD
                     LWERAQQAAQNRQQASRSAQAVYRPYLLSNLAYCDVCHRKLRSQHATGGGSYYRETSY
                     QRGFADCPNQKSGVRTELVDRQIHAIVEMIELPADWQAEIGEQLVDTAEIENIERQRT
                     RLQAEMRRMREMYRRGEYDDDVDEFHGIMKNLRRQLDQLPAERPDDSLYAVMQSIQSL
                     RDIWADASADDQRDLFRLMFQEVVVDVANARVVSLKPDPVMLPIVRQMPLLRESDWGV
                     FVPAWPPELAAQVLSVPYHPPLATLPEQTAALPFIAASPFQPSVTGARIAPQISQALE
                     RCESVNPQGWRVVQVTHPGAAPLPADLRKWPGARSELISPAQFAGLAPGSVDVLVTQY
                     LFWDAALSDQPAALAEQIARVHQVLAPGGVWLIQEPLPANSQSHWIYLALPRLADWVQ
                     GRFVDAAALYRALQTAEFEIGFDYAAAYQPITAALARTLIADRAGLLARAPLDDTADL
                     AARLPADPAAIIPSEFAFCTVWCCKAR"
BASE COUNT          514 a          732 c          854 g          486 t
ORIGIN      
        1 cacagtaccc actctctgcc gggcctgttt ttccgaacag gcccggcttt gtcactcatt
       61 tatgttcttc tcaagttaga gttcacgacc ttgcacggag gagcgatcaa ttagctgaag
      121 aactcttcca ctggataaat gcgccagggt cacctggcct tgcagcacca gacggtgcag
      181 aaggcaaatt cggaggggat gatggcggcc gggtcggcgg ggaggcgggc ggcgaggtcg
      241 gcggtatcat ccaggggggc gcgggccagc aggccggcac ggtcggcgat gagggtgcgc
      301 gccagggcgg cggtgatggg ctgataggcg gccgcatagt cgaatccgat ttcgaactcg
      361 gcggtttgca gcgcccggta gagcgcggcg gcatcgacaa accggccctg aacccaatct
      421 gccagccggg gcagggccag gtaaatccag tgcgattggc tgttggccgg cagcggctcc
      481 tggatcagcc agacgccgcc gggggccaga acctgatgca cccgggcgat ctgttcagcg
      541 agcgcggcgg gctgatcgga gagggcggca tcccagaaca gatactgcgt gaccagcaca
      601 tccacactgc ccggggccag cccggcaaac tgggccggcg agatgagttc actgcgggca
      661 ccgggccact tgcgcaggtc agcgggcaac ggggccgcgc cgggatgcgt gacctgaacg
      721 acccgccaac cctgcgggtt gactgactcg cagcgttcga gcgcctggct gatttgaggc
      781 gcgatgcgag cgccggtgac ggagggctgg aagggcgagg cggcaataaa cggcagggca
      841 gccgtttgtt cgggcagggt ggcgagcggc ggatggtagg gcaccgacag cacctgggcg
      901 gccagttcgg gcggccaggc cgggacaaaa acgccccagt ccgattcgcg cagcaggggc
      961 atctgccgca cgattggcaa catcaccggg tcgggtttga ggctgaccac ccgggcgttg
     1021 gcaacgtcaa caaccacctc ctggaacatc aaccggaaca ggtcgcgctg gtcatcggca
     1081 gaggcatcgg cccagatatc ccgcagcgac tggatggact gcatcacggc gtagaggctg
     1141 tcatcggggc gctcggcggg gagctggtct aactgccggc gcaggttttt catgatgccg
     1201 tgaaactcat ccacatcgtc atcatactcg ccgcggcggt acatctcacg catgcggcgc
     1261 atctcggcct gcaggcgggt gcgctggcgc tcgatgtttt cgatttcggc agtatcaacc
     1321 aactgttcac cgatctcggc ctgccagtcg gcgggcagtt cgatcatctc gacgatggca
     1381 tgaatctggc ggtcaaccag ctcggtgcgc acccccgatt tttggttggg acagtcggcg
     1441 aacccgcgct ggtaagacgt ttcgcggtaa tagctgccgc cgccggtggc gtgctgggag
     1501 cgcagcttgc gatggcagac atcacagtag gccaggttgg acagcaggta tggccggtaa
     1561 acggcctgcg ccgaccggga ggcctgctgc cgattttgag ccgcctgctg ggcgcgttcc
     1621 cacagatcca gggaaatcaa cggctcatgc ctgccctcgt aaatttcttt cgccttgcca
     1681 tgtttaatcc gatacccaac ccggcccgta taaaacgggt ttttgagaat gtcggtcacg
     1741 gtctctttag agaactgctg ccgcacggtc ggggtttcac cgcgccggcg cgggctgggc
     1801 cggggccgaa agccagcggc ggccagaagg tcggcgattt gctggtgcga atagcgcccg
     1861 gtggcgtact gctcatagat gaaccggacg gcgcgggctt cttcctcatt gatggtaaag
     1921 ggctggcggg ggtcttcacc ccgggcatag ccatacggcg gcaccgatgc gttatagagc
     1981 cccacacggg cgcgttggtt tttcgatttg gcggtgtgga gccggagctg cgtcaaaaaa
     2041 tactggttca tggctgccag gatgatgaga aaaaattgtt tagccgggtc ggtgaaatcg
     2101 aaatctggat ccttgaccga cgcaaaggtg atgttccact tgcccaggtc ttgcagaata
     2161 tccagcgtgt gatacaccgt gcgggagagc cgtgacagat catgcacgat caaaacatcg
     2221 aactgcctgt tgctggccag ggccatcaga ttatccagtt gcgggcggga gcgctgtgaa
     2281 cctgaaattc cccggtccac aaactcgcca accacctgat agcccttgcg ctcacaatac
     2341 tcatgcatct cggccaactg ggcctcgatg gagaaatgat cttcgccggc ctgcatttcg
     2401 gtagagacac ggccatacaa agcggcgcgg cgcttgttgt taggatcaga catgggaaaa
     2461 cctccagttt gattgaatag attctatcgt aaaacatcat ttgatggttc gggattgaac
     2521 gaggagctac tgcgaacaat ggcgattgtt agcagtcaat ttttgcacag cgggcaaaag
     2581 gctgcg
//
LOCUS       DAOPVS010000228         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_42436_length_2562_cov_30.926207, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000228 DAOPVS010000000
VERSION     DAOPVS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42436_length_2562_cov_30.926207"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..>2562
                     /locus_tag="PKL11_10985"
     CDS             199..>2562
                     /locus_tag="PKL11_10985"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNZ14167.1"
                     /translation="MPNITEAQTRRQLIDPALEKAGWYLRDHSRVKLEMPVAGYDTAP
                     WDGVTDYCLCRENGEVLAVVEAKRTVNNPQLAEAQGVYYAREIAPHQSFTPFVFLTNG
                     HEIYFLDVDRSPRRLVAGFFSPADLENLLFIRQQGAPPAQMPINNQIVDHPYQHEAIR
                     RVSEALEQGRRKALLVMATGTGKTRTAMGLIDVLLRANQARKILFVADRDALVEQAIN
                     KGFKKHIPHEPCTRLHSWNLDTSNRLYAVTLQTLSNCFEQFTPGFFDLIIFDEVHRSI
                     FNKFNEVLEYFDARMIGLTATPAQYVDRNTFNAFGCYDGKPTYLYSYDQAIADGYLVD
                     YELYAAQTHFQRQGIHGIDLSEEDRNALIEQGIDPDEIDYSGSELESVVTVPDTERRQ
                     WEEIMQVCLKDASGLPGKTIVFAITQEHALRLETTFNEMYPHYAGLVSVITHKSEYRG
                     TRIDEFTHRSKPRIAISVDMLDTGIDVPEVVNLVFMKPVQSPIKLHQMIGRGTRSDAA
                     CEQRDWLPDGHKTHFMVMDFWENNFSKDAVEVVDTSVPVLVTIFNTRLKLLEYYLDKP
                     SDPQYQRTIADLRAMLARIPTASFSVKRIFPQIESAWQDSFWSYLLTPGKLDFLRMQV
                     GPLLRLAPDVDVAAETFTSKVERLKLFLLTGKETTAIIQSIAEDVASLPQFVYEDPQL
                     APLTRLCAPTVLAQATPAQLDAVHTGLAGQMKYKRRQRSFLTLDLQDYIAISGYIMLT
                     QTGEQMYVTEYRRQVEQRILDLVADNPAIQAIQRGQPVDDWQLLELER"
BASE COUNT          577 a          834 c          610 g          541 t
ORIGIN      
        1 cgccctctgc acgagggcgt ggattgaaat gtgaccgccc tcggtgattt gctgaccgga
       61 ttcccccccc tgccgccgcc tcaactctca ccgaccagca tttgtacttt ttcaaattct
      121 ggtataagat tcacctatca cacaccgcct gagcatcccg ccaggcattt tgccatcaga
      181 ccattcgggg aacccctaat gccaaacatc accgaggcgc aaacccgcag acaactgatt
      241 gatcccgccc ttgaaaaagc cggctggtat ctccgcgatc acagccgcgt caagcttgaa
      301 atgccggttg ccgggtatga taccgcgccc tgggacggcg ttacagatta ctgcctctgc
      361 cgcgaaaatg gtgaagtgct ggcagtggtt gaagccaaac gaaccgtcaa taacccccaa
      421 ctggctgaag cccagggcgt ctattatgct cgcgaaattg ccccgcatca gagcttcacc
      481 ccctttgtct tcctgaccaa cggccacgaa atttactttt tagatgtaga ccgctcgcct
      541 cgccgtctgg tggcaggctt cttctcgccg gcagacctgg aaaacctgtt gttcatccgc
      601 cagcagggtg ccccgcccgc acaaatgccc atcaacaatc aaattgtaga tcatccctat
      661 cagcacgagg ctatccggcg ggtcagcgaa gcgttggagc agggccggcg caaagccctg
      721 ctggtaatgg ccaccggcac cggcaaaacg cgcacggcaa tgggtctgat tgatgtcttg
      781 ctgcgcgcca accaggcccg caaaatcctt tttgtcgccg accgtgatgc ccttgtggaa
      841 caggccatca ataaaggctt caaaaagcat atcccgcacg aaccctgcac ccgcctgcac
      901 tcctggaatc tggatacctc caaccgcctg tatgccgtaa ccctgcaaac tctcagcaac
      961 tgtttcgagc agttcacccc cggcttcttt gatctgatca tctttgatga agtccaccgc
     1021 tcaattttca ataaattcaa cgaggtgttg gagtattttg atgcccgcat gatcggcctg
     1081 acggccaccc ccgcccagta tgtagaccgc aacaccttta atgcctttgg ctgctacgat
     1141 ggcaaaccca cctacctcta ttcttatgat caggccattg ccgatggcta cctggtagat
     1201 tacgaactct atgccgccca gacccatttt cagcggcagg gaattcatgg aatcgactta
     1261 agcgaggaag atcgcaatgc cctgattgaa cagggcattg atcccgatga aattgattac
     1321 tcaggttcag aactggagtc tgtggtcaca gtcccagaca cagaacgccg ccagtgggaa
     1381 gaaatcatgc aggtctgcct caaagatgcc tccggactgc ccggcaagac cattgtcttc
     1441 gccatcaccc aggaacacgc cctgcgcctc gaaaccacct tcaacgagat gtacccgcac
     1501 tatgccggcc ttgtatcggt catcacccac aaatcggaat accgcggcac acggattgac
     1561 gaatttaccc atcgttcaaa accgcgcata gccatttcgg tggatatgct cgataccggc
     1621 atagacgtgc ctgaagtggt caatctggtt ttcatgaagc ccgttcagtc gcccatcaag
     1681 ctgcatcaaa tgattggccg cggcacccgc tcagatgccg cctgtgagca gcgcgactgg
     1741 ctgcccgatg gacataaaac ccacttcatg gtcatggatt tctgggaaaa caacttcagc
     1801 aaagatgccg tcgaagtggt tgatacctcg gtgcctgtgc tggtgacgat cttcaacacc
     1861 cgcctcaagc tgcttgaata ttacctggat aaaccctccg acccacagta ccagcgcacc
     1921 attgccgacc tgcgcgccat gctggcgcgc atccccacgg cttctttcag cgtcaagcgc
     1981 atcttccccc agattgaatc agcctggcag gattccttct ggtcgtatct gctcacccct
     2041 ggcaagctcg atttcctgcg gatgcaggtt ggcccgctgc tgcgcctggc ccccgatgtg
     2101 gatgtggctg ccgagacctt caccagcaag gtcgagcgcc tcaaattatt cctcctcacc
     2161 ggcaaagaaa ccaccgccat catccaatcc attgccgaag atgtcgccag cctgccgcag
     2221 tttgtctatg aagaccccca gcttgccccg ttgacccgcc tttgtgcgcc caccgtgctg
     2281 gcccaggcca cccccgccca gttggatgca gttcacaccg gtctggccgg ccagatgaaa
     2341 tacaagcgcc gccagcgctc cttcctcacc ctcgacctgc aagactacat cgccatcagc
     2401 ggctacatta tgctgaccca gaccggcgaa cagatgtatg tgaccgaata ccgccgccag
     2461 gtcgagcagc gcattttaga cctggtagcc gataatcccg ccatccaggc cattcagcgc
     2521 ggccagcccg tagatgactg gcagctcctc gaactggaac gc
//
LOCUS       DAOPVS010000229         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_42458_length_2561_cov_53.434557, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000229 DAOPVS010000000
VERSION     DAOPVS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42458_length_2561_cov_53.434557"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKL11_10990"
     CDS             complement(<1..664)
                     /locus_tag="PKL11_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14168.1"
                     /translation="MAELHPDSGVVDFAVREHRSLWLDAWRRLIASKTARLGMFIVAV
                     FVLSAVLAHFFWKYDPTVDLDYSLKLNPPTLKATGEIPMPHPFGTDKLGRDIFRRTIH
                     GGWNSLRVGIVAVSISLLIGGLLGLFAGFYESIHMIRWERILLMAAIGLALGALPAWI
                     AIQWYQAVLFMALGVASVLTVNVLTGKLWRILIFSGAGLLIGGGLGLIAAPMVGLVCG
                     VLG"
     gene            complement(673..1722)
                     /locus_tag="PKL11_10995"
     CDS             complement(673..1722)
                     /locus_tag="PKL11_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ14169.1"
                     /translation="MGRYILRRFISVLPTLLGVAFIIFMFQRLIPGDPAVAMLGEHAT
                     QENVERIREQFGLNRPAFLDREALRNGDLAGFFDSQFMRYLGRLIRLDLGTSIHRRIP
                     VSETLAERFPATLELALLSIFIALLVGLPVGIASASRRNSLLDGVTMVGSLIGVSMPI
                     FWLGLMEIMLFAIFLNWLPTGGRLTVNFDANITHLLLIDTLLKGNLPAFWDAFKHIIM
                     PALALATIPMAIIARMTRSSMLDVLQEDYIRTAKAKGLVERVVLYRHALKNAFLPVIT
                     IIGIQIGSLLAGAVLTETIFSWPGIGKWVYDSILARDYPIVQGVTLLIAIIFLLVNLL
                     VDLSYALLDPRIKYD"
     gene            complement(1807..>2561)
                     /locus_tag="PKL11_11000"
     CDS             complement(1807..>2561)
                     /locus_tag="PKL11_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ14170.1"
                     /translation="AVAHAIDKEGLIKNFFGDYGTVATNLLPPLVWGHNDAVEDWAYD
                     PELSRQLLAEAGFPDGLSEVTIAEDIMDADGNVVYKAGDVIPLRLYYMPVTRFYFPAA
                     KEIGEAAAANLVAAGFNVELYLEGDWPTYLGSRRNGLLVGLYFLGWGGDNGDPDNFTG
                     YFFGSGTEPIKREGWYQNAELAELLQEAAVTPDYATREALYKQAEQMMHDDVTRVWLG
                     HNNTPLIFLKSIKGYVPQPVGADYFEFVEIVP"
BASE COUNT          637 a          683 c          781 g          460 t
ORIGIN      
        1 ggcccagaac gccgcagacc agaccaacca tgggggcggc aatcaacccc agcccgccgc
       61 caatgagcag gccagcgccg gagaagatga ggatgcgcca taatttgccc gtcaatacat
      121 taaccgtcaa aaccgaggcg acccccagcg ccatgaacag gacggcctga taccattgaa
      181 tggcaatcca ggcgggcagg gcgcccaacg ccaggccgat cgcggccatc agcaagatgc
      241 gctcccagcg aatcatgtgg atactttcgt aaaaaccggc aaataacccc agcagaccgc
      301 cgataagcag cgaaatgctt acggcaacga tacccacacg gagcgagttc cagccgccgt
      361 ggatggtgcg gcggaagatg tcgcgcccca gcttgtcggt accgaagggg tgaggcatgg
      421 gaatttcacc cgttgccttc aaggtgggag gatttagttt taaggagtaa tcaagatcca
      481 ctgtgggatc gtatttccaa aagaagtggg ccagcaccgc agaaagcaca aaaaccgcca
      541 caatgaacat gcccagccgg gcagttttgc tggcaatcag gcgccgccag gcgtccagcc
      601 agagtgaacg gtgctcgcgt actgcaaaat caacaacgcc gctgtcagga tgtaattctg
      661 ccatgtgtat cctcagtcgt acttgatgcg tggatcgagc aaggcataag acagatccac
      721 aagcaggtta accaacagga agataatcgc gatcagcagg gtgacaccct gcacaatggg
      781 ataatcgcgg gccagaatgg agtcataaac ccatttgcca atgccgggcc aggagaaaat
      841 tgtttctgtc agaacggcac ccgccagcag agagccaatt tgaatgccga tgatggtgat
      901 gactggcagg aaggcgttct tgagcgcatg gcgataaagc acgacccgct caaccaggcc
      961 tttggcttta gccgtgcgga tgtagtcttc ctgcaggacg tccaacatgc tggagcgcgt
     1021 catgcgggcg atgatggcca tggggatggt agccagcgcc agggcaggca tgatgatgtg
     1081 cttgaatgca tcccaaaagg cggggaggtt gcctttcaag agtgtatcta tcaataacag
     1141 gtgagtaatg ttggcatcaa agttaaccgt gaggcggccg ccagttggca gccagttgag
     1201 gaagatggcg aagagcatga tctccatcaa ccccagccag aagattggca tggatacgcc
     1261 aatcagcgag ccaaccatag taacgccatc gagcagtgag ttgcgccgag atgctgaggc
     1321 tatgcccacc gggaggccga ccaacagcgc gatgaaaatg gataacagcg ccagttccaa
     1381 tgtggccggg aagcgctcag ccagcgtttc agacacggga attcggcggt ggatggaggt
     1441 gcccagatca aggcggatca ggcggcccag gtagcgcata aactggctgt cgaaaaagcc
     1501 agccaggtcg ccattgcgca gcgcttcgcg atctaaaaag gctggacggt tgaggccgaa
     1561 ctgttcgcga atgcgttcca cgttctcctg ggtggcatgc tcaccgagca ttgccacagc
     1621 aggatcaccg gggatgaggc gctggaacat aaagataatg aaggcaacgc caagcagggt
     1681 gggcaagaca gagataaacc ggcgcaggat gtagcgtccc aagtggggct cctctcttac
     1741 tgagtggcct ggggggaaga tccccccagg ccagcaatgc gtcaatgaat ccgttcggga
     1801 cggatattag ggaacgatct ccacaaactc gaagtagtca gcgccgaccg gttggggcac
     1861 ataacccttg atgctcttca agaagatcaa cggggtgttg ttgtggccca gccagacacg
     1921 ggtgacatcg tcatgcatca tctgctcagc ctgtttgtac agggcttcgc gggtggcata
     1981 atcgggggtt acagcggctt cctgcaggag ttcagccagt tcggcgttct ggtaccaacc
     2041 ctcacgtttg atgggttcag tgccggagcc gaagaagtag ccggtgaagt tgtcggggtc
     2101 gccattatca ccgccccagc cgaggaagta caggccaacc aggaggccat tgcggcggga
     2161 accgaggtat gtcggccagt cgccttcgag gtagagctcg acattgaagc cagcagctac
     2221 caagttagcg gcagcagcct cgccaatttc tttggcggcg gggaagtaga agcgggtcac
     2281 gggcatgtag tagaggcgca gcggaatgac atcgccggct ttgtagacca cattgccatc
     2341 ggcatccatg atgtcttcag caatggtgac ttcgctcagg ccatccggga aaccggcttc
     2401 ggccagcagc tggcgtgaaa gctcggggtc gtaggcccaa tcttcaaccg catcgttgtg
     2461 gccccagacc agcggaggca gcaggttggt ggcaaccgtg ccgtaatcgc caaagaagtt
     2521 cttaatcagg ccttctttgt caatggcatg ggcaacggct t
//
LOCUS       DAOPVS010000230         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_43211_length_2529_cov_43.176233, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000230 DAOPVS010000000
VERSION     DAOPVS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43211_length_2529_cov_43.176233"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /gene="priA"
                     /locus_tag="PKL11_11005"
     CDS             <1..2206
                     /gene="priA"
                     /locus_tag="PKL11_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNZ14171.1"
                     /translation="LMLPPGLSQQADTLYSLHTAPPSPAAPLTAFQQKLIALLQQRGP
                     LRGRQLTAAFPRQDWKKSASTLVRRGVLLARPVLPQPQIRPKTARTVQLACRPEEIEP
                     RLAEVGRSGSPAQQRRLSALRFLAREGAPVQVAWTYANSGCTSADLAKLAEAGLVLLG
                     ETEIWRDPLEAITYVLQEPPVLTADQQAVWAKVQTAVRAASSSQPQPPCLLHGVTGSG
                     KTEIYLRAVEETLRQNRQAIILVPEIALTPQTVRRFMARFPGRVGLVHSRLSPGERYD
                     TWRRARSGELDVIVGPRSALFAPLARLGLIVLDECHDGSYDQDDNPPSYHAVSAALTY
                     ARLTGSVIILGSATPEITQRYQAQEQGWTVLSLPNRILAHREQVSLPEGSAQSPDVTS
                     LPLPPVTIVDMRQELQMGNRSIFSRELTANLERALSAGQQAILFLNRRGSATYVFCRA
                     CGHVLKCPRCDLPLTYHVNQSGLLCHTCGYTRQMPAKCPQCKSDQIRQYGAGTERVER
                     EVQERFPAARTLRLDAETVQQKGSHEIILSHFVHHRADILIGTQMLAKGLDLPLVTFV
                     GVVLADVGLNFPDYRAGERGFQLLSQVVGRAGRSPLGGRAVIQTFNPDHYAIQLAALH
                     DYDSFYEQELEYRRQIQYPPFTRLARLEYRHARPERAEAEACSLAARLSDWLLQGGFA
                     ATDLIGPVPCFYDRVNGEYRWQIIVRGPNPAAVLRGRSLENWRIEIDPASLL"
     gene            complement(2260..>2529)
                     /locus_tag="PKL11_11010"
     CDS             complement(2260..>2529)
                     /locus_tag="PKL11_11010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ14172.1"
                     /translation="DAVRAADSGMVVYAGWIGGGYGNMVMVDHGNGYQTLYAHLSYVN
                     VGCGQPVYQGNLLGGAGSTGNSTGPHLHFEVRYMGGFINPWYVLP"
BASE COUNT          451 a          875 c          697 g          506 t
ORIGIN      
        1 cctcatgctc cctcccggcc tcagtcagca ggccgatacc ctttacagcc tgcatactgc
       61 accgccttcc ccggcagccc cactgactgc ctttcagcaa aagctgattg ccctgctgca
      121 gcagcgcggt ccgctgcgcg gccgtcagtt gacggcggcc tttccccgcc aggactggaa
      181 gaagtctgcc tcaaccctgg tgcgccgcgg cgtgctgctg gcgcgtccgg ttttacccca
      241 gccgcaaatc cgccccaaga ccgcccgcac agttcagctt gcctgccgcc cggaggagat
      301 tgagccccgt ctggctgaag ttggtcgttc gggcagcccg gcgcagcagc gccgcctgtc
      361 agcgttgcgg tttctggcgc gcgagggcgc cccggttcag gtcgcctgga cgtatgccaa
      421 ctcgggctgt accagcgccg acctggcaaa acttgccgaa gccggtctgg tgctcctggg
      481 tgaaaccgaa atctggcgtg acccgctgga agcgatcacc tatgtcctgc aagagccccc
      541 tgtcttgacg gcagatcaac aggcggtttg ggctaaagta cagacagccg tcagggctgc
      601 atccagcagt caaccgcagc ccccctgcct gctgcatggc gtcacgggtt cgggtaaaac
      661 cgaaatctac ctgcgcgcgg ttgaagaaac cctgcgccag aaccgtcagg ccatcattct
      721 ggtgcccgaa attgccctca ccccgcaaac cgtgcgccgc tttatggcgc gtttcccggg
      781 ccgcgtgggc ctggtgcatt cacgcctttc acccggcgag cgttatgaca cctggcgccg
      841 cgcccgctcc ggcgagctgg atgtcattgt tggcccacgc tcggcgcttt ttgctccgct
      901 ggcccgcctg ggcttgattg tgttggatga atgtcacgac ggctcgtacg accaggacga
      961 taatccgccc tcctatcatg cggtttctgc cgccctgacc tacgcccgcc tgacaggcag
     1021 tgtcatcatt ttgggctctg ccacacccga aatcactcag cgctaccagg ctcaagaaca
     1081 aggctggacg gtgttgagcc tgcctaaccg cattctagcc caccgtgaac aggtcagcct
     1141 gccggagggt tcagcgcaat ctcctgatgt cacctctctg cccctgcccc cggtaaccat
     1201 cgtggacatg cgccaggaac tgcagatggg caaccgctct atctttagcc gtgaactgac
     1261 tgccaatctg gagcgcgccc tgagcgccgg ccagcaggcc attttattcc tcaaccgccg
     1321 cggcagcgcc acctatgttt tctgccgcgc ctgtgggcat gtgctcaaat gcccgcgctg
     1381 cgacctgccc ctgacctatc acgtcaatca atccgggctg ctgtgccaca cctgcggcta
     1441 cactcgccag atgccggcaa aatgtccaca gtgcaaaagc gaccagatcc gccagtacgg
     1501 cgccggcacc gaacgcgtgg agcgtgaggt acaggagcgg tttcccgccg cgcgtaccct
     1561 gcggctggat gctgaaaccg tccagcaaaa aggctcgcat gaaatcatcc tctcccattt
     1621 tgtccaccac cgtgccgata tcctcatcgg cacgcaaatg ctcgccaaag ggttagacct
     1681 gcccctggtc accttcgtgg gcgtggttct ggcggatgtg ggcctcaact ttccggatta
     1741 tcgcgccggt gaacgtggtt ttcaactcct ctcgcaggtt gtggggcgcg ccgggcgcag
     1801 tccgctgggg gggcgggccg tcattcagac tttcaatccc gatcattacg ccatccagct
     1861 tgccgccctc catgattatg acagcttcta cgagcaggaa ttggaatacc gccgccagat
     1921 tcaatacccc cccttcactc gccttgcccg cctggagtac cgccacgccc gacccgaacg
     1981 cgccgaagcc gaggcgtgca gcttggcggc ccggctctcc gattggctgc tgcagggggg
     2041 ctttgccgcc acagatctga ttggtcccgt gccctgtttt tatgatcgcg ttaacggaga
     2101 ataccgctgg cagattattg tgcgcggccc caacccggca gccgtgctgc gcggtcgctc
     2161 cctggaaaac tggcgcatcg aaattgatcc cgccagcctg ttataaaaat aatcccctcc
     2221 tgaatatcgc caggagggga tttgttctat cgtctcattc tatggcagta cataccacgg
     2281 gttgatgaac ccgcccatgt accgcacttc aaagtgcagg tgtggcccgg tcgagttgcc
     2341 cgtgctgcct gcgccgccca gcaaattgcc ctggtacacc ggctgaccgc agcccacgtt
     2401 cacatagctc aggtgagcat acagcgtttg atagccgttg ccatgatcca ccatcaccat
     2461 gttgccatac ccgccgccaa tccagccggc atagaccacc atgccgctgt ccgcagcccg
     2521 caccgcatc
//
LOCUS       DAOPVS010000231         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_43233_length_2528_cov_30.629600, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000231 DAOPVS010000000
VERSION     DAOPVS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43233_length_2528_cov_30.629600"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2089)
                     /locus_tag="PKL11_11015"
     CDS             complement(<1..2089)
                     /locus_tag="PKL11_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HNZ14173.1"
                     /translation="MKKSLLSIFILVAFLLACIPPLPTQAAEDLIIYADALQNGWGNW
                     SWGAAIDPNNAAPVHSGEASIAVTITEQWGALYLGNNTPLSTSGYSGIRFWIHGGAAG
                     GQSISFHLMDDDNRIHISPQPNTWQEFTIPWADLGNPTEINSLVWQDDLGTSHPIFYV
                     DDISLVGDDSTPTPTPPPGAGPVLTIDAAADRHPISRDIYGMNFADEDLAAELRLPVR
                     RWGGNATTRYNWQTDTSNQAMDWYFENYQESPSHSRVTQFIEQDQRTSTHTILTMPLI
                     GWTPNDNPQACGFSITKYGPQQDADWSWRPDCGNGVLTGGQTITGNDPLDTSTLIGPE
                     FITDWINHLISAYGRADQGGIRFYNLDNEPMLWSSTHRDVHPNPASYDEMRDRTWAYA
                     AAIKTADPSALTLGPVTWGWTAYFWSALDWADPDFPTVTPDRTAHGGLPFTAWYLQQM
                     QAYEQANGIRILDYLDLHYYPQANGVSLSPAGNAATQALRLRSTRALWDETYADESWI
                     ADVPEGPYVRLIPRMHTWVETYYPGTKLAITEYNWGALDHINGALAQADVLGIFGREG
                     VDLAALWGPPESNQPGAFAFRMYLNYDGYHHGFGETSIQAVSTDQDQLAVYAAQRGAD
                     AALTLMIINKTGADLVSPVALTNFVPASSAQVYSYSAQDLTCIIRQPDQPVSSTGFTA
                     TYPANTITLVVIPP"
     gene            2245..>2528
                     /locus_tag="PKL11_11020"
     CDS             2245..>2528
                     /locus_tag="PKL11_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14174.1"
                     /translation="MPIDTLPGWRLWLARLLIAWTLVINLDAAVAFIIKAEAFAPGFE
                     LSGAPGAGMMRGMGILFLMWNVPYIVALIHPGKHRVSLYEAIVMQFIGVVG"
BASE COUNT          565 a          610 c          796 g          557 t
ORIGIN      
        1 agggcgggat cacgaccagg gtgatggtgt ttgccggata ggtggcggta aaacctgttg
       61 aagaaacagg ctgatcgggc tggcggatga tgcaggttaa atcctgcgcg ctgtagctgt
      121 acacctgtgc gctggatgcc gggacgaaat tggtgagggc taccgggctg accagatcgg
      181 cgccagtttt gttgatgatc atcaaggtca gggcagcatc agcgccacgc tgcgccgcat
      241 agacggcaag ctgatcttga tcggtgctga cagcctggat gctggtttcg ccaaagccat
      301 gatgatatcc atcgtaattc aggtacatcc taaaagcaaa agcgccgggc tgattgcttt
      361 ccggcgggcc ccagagagcg gcaagatcaa cgccttcgcg gccaaagatg cccagcacat
      421 ctgcctgagc cagggcacca ttgatatgat ccagagcgcc ccagttgtat tcggtgatgg
      481 cgagctttgt accgggataa taagtctcaa cccacgtatg catccgtgga atcaggcgga
      541 catagggacc ttcggggaca tcggcaatcc aggattcatc cgcataggtt tcatcccaca
      601 gcgcccgggt ggagcgcagc cgcaaagcct gggtggcggc attgccagcc ggtgaaagcg
      661 aaacgccgtt ggcctgggga taatagtgca aatcgagata atccaggatg cgaatgccgt
      721 tggcctgttc ataggcctgc atttgttgaa ggtaccaggc cgtgaaaggc aggccgccat
      781 gggcggtgcg gtctggcgtg acagtgggga agtcgggatc ggcccaatcg agggcggacc
      841 agaagtatgc tgtccagccc caggtgacgg ggccaagcgt cagcgcagac gggtcagccg
      901 ttttgatggc ggcggcataa gcccaggtac ggtcacgcat ttcatcataa gaagcaggat
      961 tgggatggac atctcgatgt gtgctggacc agagcatagg ctcgttatcc agattgtaga
     1021 aacggatgcc gccctgatcg gcgcggccat aggcactgat cagatgatta atccaatcgg
     1081 tgatgaattc ggggccaatg agggtgctgg tatccagcgg atcgtttccg gtaatcgttt
     1141 gaccgccggt caagacacca ttgccgcaat ccgggcgcca gctccaatcg gcatcctgct
     1201 gagggccata tttggtgatg ctgaagccgc aggcctgggg attatcatta ggcgtccaac
     1261 cgataagggg catggtcaaa atagtgtggg tggaggtgcg ctggtcttgc tcaataaact
     1321 gcgtgacccg agaatgactg gggctctcct ggtaattttc aaaataccag tccatggcct
     1381 gattggaggt atcggtttgc cagttgtagc gggtggtggc attgccgccc cagcggcgta
     1441 ccggcaagcg aagctcggca gcgaggtctt catcggcaaa gttcatgcca tagatatccc
     1501 ggctgatggg gtgacgatca gccgcagcat caatggtcaa cacggggccg gcgccgggtg
     1561 gcggtgtggg cgtgggggtg ctgtcgtcac caaccaggga tatatcgtcc acataaaaaa
     1621 tgggatgact ggtacccaga tcatcctgcc agaccaggct gttaatttct gtgggattgc
     1681 ccagatcggc ccaggggatg gtaaattctt gccaggtatt gggttgaggc gaaatgtgga
     1741 tgcgattgtc gtcgtccatc aaatggaagc tgatgctctg gcctccggcg gcgccaccgt
     1801 gaatccagaa gcggatgccg gaatagccgc tggtgctgag cggcgtgtta ttgccaagat
     1861 ataaagcacc ccattgctct gtaattgtaa cagcgatgga tgcttcgccg ctgtggacag
     1921 gggcagcgtt gttcggatct atcgctgcac cccaggacca gttaccccag ccattttgta
     1981 gggcatcggc gtagatgatc aaatcttcag cggcctgggt gggtaaaggc ggaatgcaag
     2041 ccagcaaaaa agcgaccaat ataaaaatag aaagcaagct ctttttcaaa atacacctca
     2101 gatcaggtcg agttattggc tcggtggaat tgtctaaaca ataactctat tataaacaat
     2161 ctgtcaatcc agagtccggt tacgactgca atggggtaaa atgaattgat gtctgcgaat
     2221 ggcccactcg cttctgacaa gataatgccg attgacacgc tgccgggctg gcggctgtgg
     2281 ctggcgcggc tcttgattgc ctggacgctg gtgatcaatc tggatgctgc ggtagcattc
     2341 attatcaagg ccgaagcctt tgcaccgggt tttgagctat cgggggcgcc gggggcgggc
     2401 atgatgcgcg ggatggggat tttgtttctg atgtggaatg tgccttatat tgtggcgctg
     2461 attcaccctg gcaaacaccg tgtgtcgctt tatgaagcca ttgtgatgca gttcattggc
     2521 gtggttgg
//
LOCUS       DAOPVS010000232         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_43683_length_2509_cov_33.179299, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000232 DAOPVS010000000
VERSION     DAOPVS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43683_length_2509_cov_33.179299"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="PKL11_11025"
     CDS             <1..1802
                     /locus_tag="PKL11_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNZ14175.1"
                     /translation="IPIVGGVLVGLINQYLIGSEKLHGTAAVMQSVALAGGRLRYQKG
                     PIRAIAAVLSIGMGASVGPEDPSVQIGANLGSMLGQKARVSDERMRTLVAAGAASAIA
                     AAFNAPIAGVFFALEIILGEIVGSSLSMILIAAVTSSVLTQAISGSQPAFQIPAYAFN
                     SVWELPLYLGLGLLAGPVSALYVRLVYKMQDIYRGWQVPRWVKTGSAGLIVGLVGLFL
                     PNIFGVGYETIGEILNVHDIALWMLFALMAAKLVLTPISIGGGFVGGVFAPALFIGAT
                     LGGGFGMIAALLLPGLQVNPAAFALVGMAAILAGAVHAPLTAAILLFEMTNDYRIILP
                     LMFAVAVSLLISQRIQKDSIYMMGLARSGIRLDRGRDVEVMSAVTVGDAMHPATETLD
                     EDLSAVEAADKLYQSRHHGLPVVDKDGWLVGILTQQDIERRTGDRVRDICTRDLETAF
                     PDETLNIALERMSQRDVGRLPVVLRENPRKLIGVLRRADVIHAYNVALTRRTAQRHQE
                     QAVRLDALTPARVEIINVRVEFGSASAGKQLSDIPFPRDCVIASLQRGSKVFIPRGAT
                     VIKPGDVLVVAAQGTARDAVIRLCQKFTDSEPE"
     gene            complement(1812..>2509)
                     /locus_tag="PKL11_11030"
     CDS             complement(1812..>2509)
                     /locus_tag="PKL11_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ14176.1"
                     /translation="EKPLVTTYRHAAELIDLAKQNQTLLMEALWTRCLPAFQQARTWV
                     ETGRIGSVKLITADFHHAVPFDPDSRLFNPRLAGGSLFDLGIYPISFATGILNEHPTK
                     ITGLAQLAPSGVDESASLALHFPSGALASLTCGFSVNAAGPARIYGAAGQIVVENCFG
                     PQRCELYDAHGSLIESFAEPTPDGFIYQIRHCVGLFQQGQIESNLIPWKDSLASAAIF
                     DALCSQWGIYSSP"
BASE COUNT          503 a          584 c          850 g          572 t
ORIGIN      
        1 tcatccccat cgtgggcggg gtgctggtag ggctcatcaa ccagtatttg attgggagcg
       61 agaaactgca tggtacggcg gcagtaatgc agtcagttgc gctggcgggc gggcggctcc
      121 ggtatcagaa agggcccatc cgggcgattg cggctgttct ctccattgga atgggagcgt
      181 cagttggtcc agaggatcca tcggtgcaga ttggggcaaa tctgggttcg atgctggggc
      241 aaaaggcgcg ggtgtctgat gagcgcatgc gcacactggt ggctgcgggt gcggcaagcg
      301 caattgcggc agccttcaat gcgccgattg cgggagtgtt ctttgcgctg gagatcattc
      361 tgggtgaaat tgtggggagt tctttgagca tgatcctgat tgcagccgtg acctcgtctg
      421 tgctgacgca ggcgatatcg ggaagccagc ccgcatttca gattccggcc tatgctttca
      481 attctgtctg ggagcttccg ctttacctgg gcctggggct gctggcgggg cccgtctcag
      541 cgctatatgt gcggttggtg tacaaaatgc aggacatcta tcgaggctgg caggtgccga
      601 gatgggtcaa gacgggcagc gccgggctga ttgtgggcct cgtgggtctt tttctgccga
      661 atatttttgg ggttgggtat gagaccatcg gggaaatttt gaacgttcat gatattgccc
      721 tatggatgct gtttgcgctc atggcggcaa agcttgtttt aaccccgatc agcattggag
      781 gcgggtttgt cggcggtgtt tttgccccgg ccttgtttat tggagcaacg ctggggggtg
      841 ggtttggaat gattgcggcg ctgctgttgc cgggattgca ggttaacccg gccgcatttg
      901 ctctggtggg catggcagct attctggctg gtgcggtgca tgcgcccctg accgcggcga
      961 ttttgctgtt tgaaatgacg aacgattatc ggattatcct gccgttgatg ttcgcagtag
     1021 cagtgagcct gttgatttca caacggattc aaaaggactc gatatatatg atggggttgg
     1081 cacgttccgg catccggctg gaccgcgggc gagatgtaga agtgatgagc gctgttactg
     1141 tgggtgacgc catgcatccg gcaaccgaga ctctggacga agacttgagc gcagtggagg
     1201 cagcagataa gctctatcaa agccggcacc atgggctgcc agtggttgat aaggatggct
     1261 ggctggtggg gattctgaca cagcaagata ttgagcgccg gacgggggac agggttcgtg
     1321 atatttgcac gcgagacctg gagactgcct ttccggatga aactctgaat attgcgctgg
     1381 aacgcatgag ccaaagggat gtggggcgcc ttccggtggt gctgcgggaa aatccgcgta
     1441 agctgattgg agttttgcgg cgggcagatg tgattcacgc atataatgtg gcgctgacgc
     1501 ggcggacagc tcagcggcat caggagcagg cggttcgtct ggatgcgctg accccggcac
     1561 gtgttgagat tatcaacgtc agggtggaat ttggctctgc cagcgcgggg aaacaactga
     1621 gcgacatacc ttttccacgg gactgtgtga ttgccagcct gcagcgcggc agcaaggtgt
     1681 tcataccgcg gggggcaacg gtgatcaagc cgggggatgt actggttgtt gcggctcagg
     1741 gaacagcccg cgacgcggtt atccggctgt gccagaaatt tacagattca gaaccagaat
     1801 aaacaacgcg ttcaggggct tgaatagatg ccccactgag aacacaaggc atcaaaaatg
     1861 gcggcgctgg ccagtgaatc tttccagggg attagattgc tttcaatttg accctgttga
     1921 aacagcccga cacagtggcg gatctgatag ataaaaccat cgggcgtggg ttcggcaaag
     1981 ctttcaatca ggctgccatg ggcatcatac aattcgcagc gttgagggcc aaagcaattt
     2041 tcaaccacaa tctgtccggc cgcgccataa attctggctg ggccagccgc attgacgctg
     2101 aagccgcagg tcaggctggc gagagcgccc gagggaaagt gcagggcgag cgaggcggat
     2161 tcgtccacgc cgctgggggc aagctgggcc agcccggtta tttttgtggg atgctcgttc
     2221 aggataccag tggcaaagct gatgggatag atgcccaggt caaacaggct gccacctgcc
     2281 aggcgcggat tgaacaggcg gctgtcgggg tcaaaaggca cggcatggtg aaagtcggct
     2341 gtaatcagtt taacggaacc gattctaccg gtttcaaccc aggtgcgggc ctgctgaaaa
     2401 gccgggaggc agcgcgtcca gagggcttcc atcaggaggg tttgattctg ttttgcgagg
     2461 tcgatgagtt cggccgcgtg gcggtaggtt gtgacaagcg gtttttcgc
//
LOCUS       DAOPVS010000233         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_45026_length_2458_cov_61.164378, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000233 DAOPVS010000000
VERSION     DAOPVS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45026_length_2458_cov_61.164378"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="PKL11_11035"
     CDS             complement(<1..2071)
                     /locus_tag="PKL11_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNZ14177.1"
                     /translation="MPLLWLSLAFLAGLGAAAALGAGLWFWAGLSLAALALSILEARF
                     RPAWRWLAHWRRFGRILAGLVLLAFCLGGLRYVLHQPAWQPRDLAYYNDSGSLRLVGV
                     ISAPPDLRDASVLVRVRVEHLTSDSAPDKPVRGVAQVFLLPGDWRYGDRLELIGEPQT
                     PGENDEFSYRDYLAGQGIHSVLYQPRVRLLAHNAGNPLLSAIYSLRASAHARINQLYP
                     QPEAALLSGILLGLESDIPADVQAAFRVTGTAHIIAISGFNISILSGLFLLLFSRLLP
                     RPWAALAAVLTITAYTILVGADPPVTRAAIMGAVGLGGLLLGRRQVGINSLAFTAAVM
                     SLFNPLLPWNPSFQLSFTATLGMILYAGPFQSAFANWAERRLGERWARRLAAPVGEYL
                     LTTLAAQLTTLPVIWAQFRQISLVSLIANPLILPPQPLVMTLGGVSVIAALIFLPLGR
                     LLAAITLPLLSYTIHLAEWLAGAPLAAIPLSDAPWFFAALFYASLLFLTFGRRQPRLR
                     RWLKPATQIVLAALLAGLLWRGALSAPDGRLHLTALGLDGGRAVLIQTPGGASILVDA
                     GPKTTLLSDALGRRLPLFDRSLDAVILTDRSAAALAGLPRTLERYPAAQIYWNAQSRA
                     AARRVETSLRALAVPTAPLLPGQAWDLGDGARLTVLADSPTGTALLVEYGAFRALLPG
                     NFAPADLP"
     gene            complement(2076..>2458)
                     /locus_tag="PKL11_11040"
     CDS             complement(2076..>2458)
                     /locus_tag="PKL11_11040"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNZ14178.1"
                     /translation="QPWRMLMLALQGACAAGILITFGQTGALAFLGLDALWIPQARQA
                     PPILRTDGLYRWVRHPVYTLSLLALYLMPWMTWNWLALALGATLYITLALPLEESKLI
                     DEFGEAYVTYRQRVPALLPYRLPK"
BASE COUNT          549 a          700 c          901 g          308 t
ORIGIN      
        1 cgggcaggtc ggcgggggcg aaattgcccg ggagcagggc gcggaaagcg ccgtattcga
       61 ccaataacgc ggtgccggtg ggggaatcag ccagaacggt caggcgagcg ccatcgccca
      121 ggtcccaggc ctggccgggc agcaggggcg ccgtgggaac agcgagggcg cgcaggctgg
      181 tttctacccg gcgagccgcg gcgcgggatt gggcattcca gtaaatctga gccgcggggt
      241 agcgttcgag ggtgcgcggc aggccagcca gggcagcagc cgagcggtcg gtgagaatga
      301 cggcgtccag agagcgatca aagaggggca ggcggcggcc cagcgcatcg gagagcaggg
      361 tcgttttagg gcccgcatcc accaggattg aggcgccgcc aggcgtctgg atgaggactg
      421 cccgaccgcc atccaggcct agagcggtga gatgcagccg accgtccggc gccgacaatg
      481 cgccgcgcca gagcaagcca gccaggagcg cggccagcac aatttgggtg gcgggtttaa
      541 gccagcgccg caggcggggc tggcggcggc caaacgtcag gaagagcagt gaggcgtaga
      601 aaagcgccgc aaaaaaccag ggggcatcac tcaatgggat ggccgccagc ggcgcgcctg
      661 ccagccattc agccaggtgg atggtgtaac tgagcagcgg cagggtgatg gcggcgagca
      721 ggcggcccag gggaaggaaa atcagggcgg cgataacaga gaccccgccc agggtcatca
      781 ccagcggctg cgggggaaga atcaacgggt tggcaatcag gctgacgagg gaaatctggc
      841 ggaactgtgc ccagataacc ggcagggtgg tgagctgagc cgccagggtg gtgagcaaat
      901 actcacccac cggagcagcc aggcggcgcg cccagcgctc acccaggcgc cgttcagccc
      961 aatttgcaaa cgcagattgg aagggtccgg cgtataaaat catgccgagt gtggcggtga
     1021 aggaaagttg aaagctggga ttccacggca gcagtggatt aaagaggctc atcaccgcgg
     1081 cggtgaaggc caggctgttg atgcccacct gccgccgccc cagcagcagc ccaccaagac
     1141 ccaccgctcc catgatcgcc gcgcgcgtga cgggtgggtc ggcgccgacc aggatggtgt
     1201 aggcagtgat ggtgagcact gccgccaggg ctgcccaggg acgcggcagc aggcgggaga
     1261 acagcagcag aaacagacca ctgaggatgg aaatgttgaa gccggagatg gcgatgatgt
     1321 gagcggtacc ggtgacccgg aaagccgcct ggacatccgc cgggatgtcg ctttcgaggc
     1381 cgaggagaat gcccgaaagc aaagcagcct cgggctgggg atagagttga ttgatgcggg
     1441 catgggcgct cgcgcgcaag ctgtagattg cggatagaag gggattgccc gcgttgtgcg
     1501 ccagcaggcg gacgcgcggc tgataaagca ccgaatgaat gccctgaccg gcgagataat
     1561 cccggtagga aaattcatca ttttcgccgg gggtttgagg ttcgccgata agctccagac
     1621 ggtcgccgta gcgccaatct ccgggaagca gaaagacctg ggccacgccg cgcacgggtt
     1681 tatccggggc ggaatccgag gtcaggtgct ccacccgaac acgtaccaga accgaggcgt
     1741 cacgcagatc gggcggggct gaaatgactc ccaccagacg caggctgccc gaatcgttat
     1801 agtaggccag gtcccgcggc tgccaggcgg gctgatggag gacataacgc agtccgccca
     1861 ggcagaacgc cagcagaacc aggcccgcca gaattcgtcc aaagcgccgc cagtgcgcca
     1921 gccagcgcca ggcgggccgg aaacgagcct ccaggatgga cagggccagc gccgccaaac
     1981 tcagacctgc ccagaaccac aggcctgcgc cgagcgcggc tgcggcgccc agaccagcca
     2041 gaaacgcgag ggaaagccag agcagcggca tggggttact tcggcaagcg gtagggcagc
     2101 agggccggaa cgcgctgacg ataggtcaca taggcctcgc cgaattcgtc aatcagtttg
     2161 gattcttcaa gcggaagagc cagcgtaata taaagggtgg cgccaagagc cagcgcaagc
     2221 cagttccagg tcatccaggg catgaggtag agggcgagga gtgaaagggt atagaccggg
     2281 tggcgcaccc agcggtagag gccatcagtg cgcaggatgg gtggggcttg ccgggcctga
     2341 gggatccaga gggcatccag gccgaggaag gctaaagcac ccgtctgtcc aaaggtaatg
     2401 aggatgcccg cggcgcaggc gccctggagc gccagcatca acatgcgcca gggttggg
//
LOCUS       DAOPVS010000234         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_45191_length_2452_cov_53.508552, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000234 DAOPVS010000000
VERSION     DAOPVS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45191_length_2452_cov_53.508552"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PKL11_11045"
     CDS             complement(<1..1673)
                     /locus_tag="PKL11_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14179.1"
                     /translation="MKTKLVFLAIILVLAVSLTGCGKTAVPTDAPAAQVTEAPAATPM
                     PNGPALGDPIRNEAGGYAFRPIPDYSIETDGAAVMLLAPDGSLDRGPAIMLMGSNGEF
                     LNGLTAADVLTQFVGEMPFDMGAVEALSPQGVDGATAELTGKSESQGLLGRAAVLMPT
                     ADQALLMIGVAPADRWEALAPYFEAILNSIAFFEPNAAAGQTPVEAGELSLPTGYEEY
                     LAIELLSPVQDVITRLGEPTQTLSGHPYREYMLQSTIYRFQYADAAIEVIANPGGQVV
                     YKALKSIPEGRLPTTDQQAQVQPGMTLSEVEGALGEGYLFTAYVSAQDTSKQYAVYAW
                     PGSTGEGQFAVMLLGDAVIKTLLDVPGIEPAADNDTTHPRVARLLTPPTNSYESVVMA
                     NPDYVKFVNLPLGSTLDEIKAVFGEPARETVSPDGAAIKYITYIYNSATAGEFGFKIK
                     QADPNGLLLQDKDGQLIHKYASYLKEDSDMRTRHVLQVKKDLTLEEIARIMGGEGRIY
                     EEKYDDTGVIITTYFWFGTQYSTGVSALFREGEMTAYQINSSAQDDRYEL"
     gene            complement(1851..2432)
                     /locus_tag="PKL11_11050"
     CDS             complement(1851..2432)
                     /locus_tag="PKL11_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14180.1"
                     /translation="MKIKSALLLTLLVLAIGLSGCGQIVATPEAPTATPTPTGPTMGD
                     RLINNPGGFSFLSIPDYTLQADGAAVMMLAPEADVNEGPAIVLMGAVDETLTGLTAAD
                     ELPMFIEGTPFEMGEIKVITLNGLDGATVELTGSAESGGLVGRAAVLVPAPNRAFLMM
                     GVAPAERWDELAVYFEAVLGSVAFFDPAAGSTP"
BASE COUNT          499 a          767 c          640 g          546 t
ORIGIN      
        1 agctcatacc gatcatcctg cgccgagctg ttgatttgat aggcggtcat ctcgccttct
       61 cgaaacagtg ccgaaacgcc tgtggaatac tgcgtgccaa accagaagta ggttgtgatg
      121 atgacgccag tatcatcata tttttcctca tagatgcgcc cctctccgcc catgatgcgg
      181 gcaatctctt ccagcgtcag atctttttta acctgcaaga catgccgggt gcgcatatcg
      241 ctgtcctctt tgaggtaaga ggcatatttg tgaatcaact gtccatcctt atcctgcagc
      301 agcagcccat tggggtccgc ctgcttgatc ttgaacccaa actcaccggc tgtcgcactg
      361 ttatagatgt aagtaatgta cttgatcgcc gccccgtccg gtgagactgt ttcgcgcgcc
      421 ggttcaccaa acaccgcctt gatctcatcc agtgtgctgc ccagcggcag gttcacaaat
      481 ttgacataat ccgggttggc catcactacg ctttcataac tgttggtggg cggcgtcagc
      541 agccgggcca cccgcgggtg ggtggtatcg ttatcggctg caggctcaat tccgggcaca
      601 tccagcagcg tcttgatgac ggcatctccc agcagcatga ccgcaaattg cccttcgcca
      661 gtgctgcccg gccaggcata caccgcatac tgcttgctgg tatcctgcgc actcacatag
      721 gctgtgaaca gatagccttc acccagcgcg ccctccactt cactcagcgt catgccgggc
      781 tgcacctgcg cctgctggtc tgtggtgggc agcctgccct cgggaatgct ctttagcgcc
      841 ttatacacca cctgtccgcc cgggttggca atcacttcaa tcgccgcgtc ggcatactgg
      901 aagcggtaga ttgtggactg gagcatatac tcacggtaag ggtgcccgct cagtgtttgg
      961 gttggctccc ccagccgtgt gatgacatcc tgcaccgggc tgagcagttc aattgccaga
     1021 tattcctcat acccggttgg cagcgagagc tctccggcct caactggcgt ttggccggca
     1081 gcggcattgg gttcaaaaaa cgcaatgctg ttcaaaattg cctcaaaata gggcgccagc
     1141 gcttcccagc ggtcggccgg ggccaccccg atcattaata atgcctggtc tgctgtgggc
     1201 atcagaacgg cggcccgtcc cagcagtccc tggctttcag atttgccggt cagctccgct
     1261 gtggcgccat ccactccctg aggcgaaagg gcctccacgg cccccatatc aaagggcatt
     1321 tcgcccacaa actgggtcag cacatcagcc gcagtcaggc cattcagaaa ctcgccattt
     1381 gagcccatca gcattatggc tgggccgcgg tccaggctgc catccggcgc cagcagcatc
     1441 acagccgccc catctgtctc aatagaataa tctgggatcg gacgaaacgc atacccgcct
     1501 gcctcattgc ggatcggatc gcccagtgcc ggtccattcg gcattggggt ggcagccggg
     1561 gcctcggtga cctgcgctgc cggggcatcg gtgggcactg ctgtcttccc gcagcccgtc
     1621 aggctgaccg ccagaaccag aattatcgcc agaaatacaa gctttgtctt catcgaaaat
     1681 atcctttcat aaaacatcga acggattgcg cctgcctcaa cagcacacgc cccaaaagta
     1741 cactttgagt ttagcctccc agcgtcaaaa aaacgtcaaa aatgcccgct ggttacagcg
     1801 ggcattcagg ttcatgtatc tgcaaagcga tttttatcgc caggtgtaga tcagggtgtg
     1861 cttcctgcag ccgggtcaaa aaacgctact gagcccagca ccgcttcaaa atagaccgcc
     1921 aactcgtccc agcgctctgc gggagccact cccatcatca agaaagcccg gtttggtgct
     1981 ggcaccagca cggccgcgcg tcccaccagg ccgccgcttt ctgccgaccc tgtcagttca
     2041 accgtggccc catccagccc gttcagcgtg atgaccttaa tttcacccat ttcaaatggt
     2101 gtgccttcga taaacatggg cagttcgtcc gctgcggtga gccccgtcag ggtctcatcc
     2161 accgcgccca tcaacacaat cgccgggcct tcgttcacgt cggcctcggg cgccagcatc
     2221 atcaccgctg ccccatccgc ctgcagggtg taatcaggga tggataggaa gctgaatccc
     2281 cccggattat tgatcaaccg atcgcccatg gttggcccgg tgggggtggg ggttgccgtt
     2341 ggtgcttcag gcgtggcaac aatttgaccg cagccgctta agccaattgc cagaacgagc
     2401 agggtcagga gcagtgccga tttgattttc atttgatatt atccttatta at
//
LOCUS       DAOPVS010000235         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_45299_length_2448_cov_58.904722, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000235 DAOPVS010000000
VERSION     DAOPVS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45299_length_2448_cov_58.904722"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKL11_11055"
     CDS             <1..786
                     /locus_tag="PKL11_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ14181.1"
                     /translation="RFFDTAEVYGQGQSELILGKLLKTTDQPLRVATKFMPFPWQLSR
                     RVLLRHLRASLKRLDLWKVSLYQMHFPNPPVSVETWMEAMAEAVHNGLTEAVGVSNYD
                     RSQMQRAFDTLARQGISLASNQVEYHLLDRRIETSGMLQQCRELGVTVIAYSPLAQGM
                     LTGKYTAENPPRGFRAGKYTRKTLAAIKPLVDTLKKIGSDHAGKTAAQVALNWTICKG
                     TVPIPGAKNLAQAEQNAGAMGWRLTDEEVARLDELSERVTKAD"
     gene            843..1040
                     /locus_tag="PKL11_11060"
     CDS             843..1040
                     /locus_tag="PKL11_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNZ14182.1"
                     /translation="MEATRALRRSRSNRWVAGVCGGLGEFFGIAPGWFRLAFFLMLLP
                     GGIPGLVIYLICMIIIPPARA"
     gene            complement(1094..1306)
                     /locus_tag="PKL11_11065"
     CDS             complement(1094..1306)
                     /locus_tag="PKL11_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNZ14183.1"
                     /translation="MIKKVFRILDMECPACAMRLEGLEDDVPGIRQVSASYHKQTMQV
                     EFDEQVLSEAEIIAAAQKLGYTARVE"
     gene            complement(1303..2397)
                     /locus_tag="PKL11_11070"
     CDS             complement(1303..2397)
                     /locus_tag="PKL11_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNZ14184.1"
                     /translation="MNIWIAFITGLTAGGLSCLAVQGGLLASSLAVQLEQDLQQGGKN
                     GQRSHQIARPILFFLLAKLIAYTLLGFLLGLLGSVLQLTPMMRAVLLIAIGIFMVGNG
                     LRMLNVHPIFRYFAIQPPAKFTRYLRRKAKGGNASTISAVFLGALTVLIPCGVTQSMM
                     ALAISTGNPWEGAAILSAFILGTSPVFFAAFYFAAKLGAKLERAFMTIVAVVVLILGL
                     VSIESGLVLSGSPISFSRLTRPLFAQQASEETPLPTPNISPLLNPPGVDPAYTPTPAP
                     DGVITLNVLNSGYSPNSQVVPANQDLELHLITQKTYSCARAFVIPDLNVEVMLPETGD
                     VAVEIPAQPAGTVLRYACSMGMYSGELVFE"
BASE COUNT          553 a          732 c          642 g          521 t
ORIGIN      
        1 cgcttctttg acacggcaga agtttatgga caggggcagt ctgaactgat cctcgggaaa
       61 ttgctcaaaa cgaccgatca gcctttgcgt gtagccacta aattcatgcc cttcccctgg
      121 cagctcagcc gccgcgtctt gctgcgccat ttgcgggcca gcctcaaacg cctggattta
      181 tggaaggtca gcctgtacca aatgcacttt cccaatcccc cggtcagcgt cgaaacctgg
      241 atggaggcca tggctgaggc cgtccacaat ggcctgaccg aagcagtggg ggtgtccaac
      301 tatgaccgct ctcaaatgca gcgcgcgttt gataccctcg cgcgccaggg tatctcgctg
      361 gcttccaacc aggtggaata tcatctcttg gatcgccgca tcgaaaccag tggcatgttg
      421 cagcagtgcc gtgaacttgg cgtaacggtc attgcctaca gtcctctggc ccagggcatg
      481 ttgacgggca aatatactgc cgaaaatccc ccgcgcggct ttcgcgccgg caaatacacc
      541 cgtaaaacgc tggctgccat taaacccctt gtggacacgc tcaagaaaat tggctcagac
      601 catgccggca aaactgcggc tcaggtggcc ctcaactgga ccatctgcaa gggaactgta
      661 ccgattcccg gcgccaagaa cctggcccag gccgagcaga acgcgggcgc aatgggctgg
      721 cgcttgaccg atgaggaagt cgcccgtctg gatgaactca gcgagcgtgt gaccaaagcc
      781 gactgacccc cgcaacttac cccgctggct tgcgtatgta ttctctgaaa ggagttttac
      841 acatggaagc cacgcgtgct ttacgtcgtt cacggtcaaa ccgttgggtc gcaggtgttt
      901 gcggcggcct cggtgagttt tttggcatcg ctccgggttg gttccggctg gcgtttttcc
      961 tcatgctcct gcctggcggt attcctggcc tggtcatcta cctgatctgc atgatcatca
     1021 ttcccccggc gcgggcatag atccctcccg cgcgtacagg cggattggat gctggtcgtc
     1081 caatccgcct tttttattcc acccgggcgg tgtatcccag cttttgagcc gccgcaataa
     1141 tctctgcctc actgagaacc tgctcgtcaa actcaacctg catggtctgt ttgtgataac
     1201 tggcgctgac ctgccggatt cccggaacgt catcttccag cccttccagt cgcatggcac
     1261 aggccgggca ttccatatcc aaaatccgaa agaccttctt aatcattcga aaaccaattc
     1321 tccactgtac attcccatcg agcaggcata ccgcagcact gttccggcgg gttgggccgg
     1381 gatttccacc gccacatccc cggtttcagg caacatcacc tcaacattca aatccggaat
     1441 gacgaaagcc cgcgcacagg aatatgtttt ttgagtgatc aggtgcagtt ccaaatcctg
     1501 gttggctggc accacctgcg agttggggct gtatccgctg ttcaagacat tcagcgtgat
     1561 caccccatcc ggcgccgggg tgggggtgta agctggatca acccccggcg gattaagcag
     1621 cgggctgatg tttggcgtgg gaaggggcgt ttcctcgctg gcctgttgtg caaacagcgg
     1681 tcgggtcagt ctggagaacg aaatcggtga cccggataac accaggccgc tttcaataga
     1741 aaccagccct aaaatcagca ccaccacagc cacaatcgtc atgaaagcgc gctccagctt
     1801 ggcccccagc ttggcggcaa aataaaaagc ggcgaagaaa accgggctgg ttcccaggat
     1861 gaaggccgag agaatcgccg ctccctccca ggggttgcca gtgctaattg ccagcgccat
     1921 catgctttgc gtcacaccgc acgggattaa gaccgtcaaa gcgcccagaa atacggcgct
     1981 gatggttgat gcattgccgc ctttagcctt gcgccgcagg taacgcgtga acttggctgg
     2041 cggctgaatg gcaaaatagc gaaaaatggg atggacattc aacatgcgca acccattccc
     2101 caccatgaaa atgccaattg caatcagaag gacagcccgc atcatcggcg taagttgcag
     2161 caccgaccct aacagcccca gcagaaaacc cagcagggtg taggcaatca acttggccag
     2221 caggaagaac aggattgggc gggcaatctg atgagaacgc tgaccatttt tgccgccttg
     2281 ctgtaaatcc tgctccaact gtaccgccag cgaacttgcc agcagcccgc cttgaaccgc
     2341 caggcagctc aacccgccag cagtcaaccc ggtaataaat gcgatccaaa tgttcatctt
     2401 tatcctttat tgataatccg ataaaatata tcttattata gagccctg
//
LOCUS       DAOPVS010000236         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_45394_length_2445_cov_33.538494, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000236 DAOPVS010000000
VERSION     DAOPVS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45394_length_2445_cov_33.538494"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKL11_11075"
     CDS             complement(<1..646)
                     /locus_tag="PKL11_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ14185.1"
                     /translation="MGRLIVLGTANAVANEHHENTHFVLLVGERAIMVDCVGNPVIRL
                     RQVGIDFNQVTDLILTHFHPDHVSGMPLLLMDMWLMQRKRPLHIYGLKHTIDRAITTM
                     TLYEWETWPNFFPVIFHRLPDEEKSAILDEPEVRIFASPGEHHIPTIGIRIEFPNIAR
                     SVAYSSDTEPCDAIARLAENVDVLIHEASGASVGHSSAAQAGEVAQRAGAQQLYL"
     gene            complement(673..2091)
                     /locus_tag="PKL11_11080"
     CDS             complement(673..2091)
                     /locus_tag="PKL11_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNZ14186.1"
                     /translation="MLKSLYPVYVIAHREIRDQFRDWRIVFPIIILTILFPFLMNYTA
                     QEMLGFVGKYGGEIVGERLIPFLLMIVGFFPSSFSLIIALETFVGEKERGSIEPLLST
                     PLEDWQLYLGKLSSAVVPPLFGSFLGMTVYLTGLAIGRAPLPDGEMMTLIILLTIVQA
                     VMMVSGAVVVSCQATSVRAANLLSSFIIIPVTLLIQGESMLMFWGNYGTLWMAFFGVI
                     LLTFLLMRMGLAYFRREELLGREIDVLNLRWGWMTFWQAFTGGAKNIWQWYRRVIPKT
                     LAALRLPFVIVFLMGAAGVAIGFSLVRQFPIIAPPEMFTNLEERLGLLTEAFKPGTFG
                     PVLMIFGNNLRVMALALLLGIFSFAVLGVLPIIVTLAVVGYILGILGLNGLPAGAFFA
                     GLILPHAWLEIPAALLATAAVLRSGALLATPAQGKKIGQVWLESLAVWAQMMIGIVIP
                     ALFIAAMIEVWITPHLASLILR"
     gene            complement(2084..>2445)
                     /locus_tag="PKL11_11085"
     CDS             complement(2084..>2445)
                     /locus_tag="PKL11_11085"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HNZ14187.1"
                     /translation="TEAEDLADQIAIIQRGRIIFNDTSANLKRRLLGAPEYEARLAGV
                     VNGWEPTNIPGVSIVQRASDVLRFRVEDPQSANPLLLEQMIAHRLPIVSFQEVHRSLE
                     DAYLEAVRQAAAEVPHA"
BASE COUNT          644 a          617 c          737 g          447 t
ORIGIN      
        1 ccaggtaaag ctgctgcgcg ccagcacgct gggcaacctc acccgcttga gccgctgatg
       61 aatgtcccac agaggcgcca ctggcctcat gaatgaggac atctacattt tcggcaaggc
      121 gggcaatggc gtcgcatggt tcggtatctg aggaataggc cacggagcgg gcaatgtttg
      181 gaaattcgat gcgaatgccg atggttggaa tatggtgttc gccaggagag gcaaaaatac
      241 gtacttcggg ctcatcgagg atggcggatt tttcttcatc cggcaggcgg tgaaaaatga
      301 ccgggaaaaa gttgggccag gtttcccatt catagagcgt catagtggta atggcacggt
      361 cgatggtgtg tttgaggcca tagatatgca gcgggcgctt gcgctgcatg agccacatgt
      421 ccatcaacag gaggggcatg ccggagacat ggtcaggatg gaagtgagtc aagatgagat
      481 cggtcacctg gttgaagtca atgccaacct gacgcaaacg gattacgggg ttgcccacgc
      541 agtccaccat aattgcgcgt tcgccaacca gcagcacgaa gtgagtgttt tcgtggtgtt
      601 cattggcaac tgcgttagct gtgcctaaaa caatcaagcg ccccatagga tcgcgtctcc
      661 tcgctcttta ttttaacgca aaattaaaga tgcgaggtgc ggagttatcc aaacttcgat
      721 cattgcagca ataaacaggg cgggaatcac gattccaatc atcatttgcg cccagactgc
      781 caatgattcg agccagacct ggccaatctt tttgccctgg gcgggggtcg ccagcaaggc
      841 gcctgaacgc aatacggctg cggttgccag aagggcagca gggatttcga gccaggcgtg
      901 gggcagaatg aggccagcga aaaaagcacc cgcaggcaga ccgttcaaac cgaggatgcc
      961 cagaatgtag ccgacaacag ccagcgtgac aatgatgggc aagacaccca agaccgcaaa
     1021 tgagaaaatg cccagcagca aagccagtgc catgacccgc aggttgttgc caaagatcat
     1081 cagcaccggg ccgaaagtgc cgggtttgaa ggcttcagta agcaggccga ggcgttcttc
     1141 gagattggtg aacatttcgg gcggagcaat aatagggaac tgacgcacca gggagaaacc
     1201 gatggcaaca cccgcagcgc ccataagaaa gacaatcaca aatggcaagc gcaaagcagc
     1261 cagtgttttg gggatgaccc ggcgatacca ctgccagatg tttttggcgc cgcctgtgaa
     1321 agcctgccag aaggtcatcc aaccccagcg caggttgagg acatcaatct cacgccccag
     1381 cagctcttca cggcggaagt atgccaggcc catgcgcatc agcaggaagg tgagtagaat
     1441 gacgccaaag aaggccatcc agagggtgcc ataattgccc cagaacatca acatggattc
     1501 gccctggatc agcagggtaa cggggataat aatgaagctg gacaacaggt tggcagcccg
     1561 cacagaggtg gcctgacagg aaacaaccac ggcgcccgaa accatcatga cagcctggac
     1621 gatggtcaat aaaatgatga gggtcatcat ttcgccatcg ggcaaggggg cgcggccaat
     1681 tgccagtcca gtcagataga ccgtcatgcc gaggaagctg ccaaagagcg gaggaaccac
     1741 cgccgaggaa agcttgccga gatacaactg ccagtcttcc agcggggtgc tgagcagcgg
     1801 ttcgatggag ccgcgttctt tttcaccaac aaaggtttcc agggcaataa tcagcgagaa
     1861 ggagctgggg aaaaagccca caatcatcag caggaagggg atcaaacgct ccccgacaat
     1921 ttcaccgccg tacttgccga caaagcccag catttcctgg gcagtgtaat tcatcaggaa
     1981 tgggaagagg atggtgagga tgatgatggg gaagacaatg cgccagtcac ggaactggtc
     2041 acggatttcg cggtgggcaa taacatagac cggatagagg gatttaagca tggggcacct
     2101 cggcggcggc ctgacgaacg gcctcaaggt aggcatcttc aagactgcga tgaacctcct
     2161 ggaaagagac aatcggcagg cgatgggcga tcatttgctc aagaagcagc gggttagcag
     2221 attggggatc ttcaacccga aatctgagga catcacttgc gcgctgaacg atggagacgc
     2281 ccggaatgtt ggtgggttcc caaccattga ccacgccagc caggcgggcc tcgtattcgg
     2341 gagcgcccag caggcgccgt ttgaggttgg cggatgtatc gttaaagatg atgcgcccgc
     2401 gctggatgat ggcaatttga tctgccagat cttcggcttc ggtga
//
LOCUS       DAOPVS010000237         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_45501_length_2441_cov_53.874267, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000237 DAOPVS010000000
VERSION     DAOPVS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45501_length_2441_cov_53.874267"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKL11_11090"
     CDS             complement(<1..857)
                     /locus_tag="PKL11_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNZ14188.1"
                     /translation="MANETPVIIRTRREAARPKQFTFTADQVRTAGLEAGLQQYRQQA
                     WQAFSATPLPDTSQEAWRRTDLRSLPVERFHLIQPDSPALPPAPKSLLKPLADDAPGG
                     QMILTPTGAQVTLNPDLAAQGVIFTDLRTAERQHPALLSRLIGQVVRADEGKFSALTA
                     ALAQTGVLLYVPSGVQVAQPLHSILWAPGVDLAHISHVIVWLEAGASATFIHESASPT
                     EPGPALHAGIVEVQVGPGANLRFVELQSFGEHVWNFTHERARVEQDGNLEWIFGAIGG
                     HLTKNFTDL"
     gene            complement(886..2298)
                     /gene="sufB"
                     /locus_tag="PKL11_11095"
     CDS             complement(886..2298)
                     /gene="sufB"
                     /locus_tag="PKL11_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNZ14189.1"
                     /translation="MSDKPQSDILNELTDYQYGFSDPDTSVFKTRRGLDSDVVRQISA
                     LKGEPEWMLEFRLRALEHYDARPMPSWGADISGLNLDDIYYYVRPTDSQGRTWDDVPD
                     TIKNTFDRLGIPEAERKFLAGVGAQYESEMVYHSIQEHLEKQGVIFLSIEDGLRQHPD
                     LFRQYFGTVVPIEDNKFAALNSAVWSGGSFVYVPPGVKVDLPLQAYFRLNMASIGQFE
                     RSLIIADEGAQVHYVEGCTAPQYSEASFHSGVIEIVVRKGARVRYTTIQNWSNNVYNL
                     VTQRARVEENGTMEWVDCNLGSKVTMKYPSCYLMGPGAHGEILSMAFAGPGQQQDAGG
                     KMVHFAPHTSSKITSKSISKGGGRSSYRGLLRVHKGAEHVRSNVVCDALLLDPQSRSD
                     TYPYIEVDEQDVTIGHEATVSKVGEEQLFYLMSRGLTQEEATTMVVSGFIEPLVKELP
                     MEYAVEMNRLIQLQMEGSVG"
BASE COUNT          515 a          663 c          754 g          509 t
ORIGIN      
        1 tctagatcgg tgaagttttt ggtcagatga ccgccaatgg cgccaaagat ccattccaga
       61 ttgccatcct gctccacgcg agcgcgctcg tgggtaaagt tccaaacatg ttcgccaaag
      121 gattgcagct cgacaaagcg gaggttggcg cccgggccta cctgaacttc gacaatgccg
      181 gcgtgcagcg ccgggccggg ctcggtgggg gaggcagatt cgtgaataaa ggtagctgaa
      241 gcgccagcct ccagccagac gatgacatgt gagatgtgcg ccagatccac accgggcgcc
      301 cagagaatgc tgtgcaaagg ctgggcaacc tgaacaccac tcggcacata gagcagcacg
      361 ccggtttggg ccagggccgc cgtcagagcg ctgaatttgc cttcgtctgc ccggacgacc
      421 tgcccgatca ggcggctgag cagggcaggg tgctgccgtt ctgcggtgcg caggtcggta
      481 aagatgacgc cctgggctgc caggtcgggg ttcaaagtca cctgggcacc ggtgggggtc
      541 aaaatcattt gaccgccggg ggcatcatcg gcgaggggtt tgagcagtga tttgggcgcc
      601 ggcggcagcg ccgggctgtc gggctggata agatggaagc gctccacagg gagggagcgc
      661 aaatcggtac gccgccaggc ttcctgagag gtatcgggca gcggggtcgc tgaaaatgcc
      721 tgccaggctt gctgacggta ttgctgcaga ccagcttcca ggccagccgt gcgaacctga
      781 tcggcagtaa aggtgaattg ttttggacgg gcagcttcgc gccgcgtccg gatgatgaca
      841 ggggtttcgt ttgccatgct tgttcctatt atcccattcc ggcggctaac cgaccgaccc
      901 ttccatttga agctggatca ggcggttcat ttcaacggca tattccatcg gcagttcttt
      961 gaccagtggt tcgatgaagc ccgagacgac catggtggtg gcctcttctt gagtcagacc
     1021 gcggctcatc agatagaaca actgctcttc gccgacttta gagacggtgg cctcgtgccc
     1081 gatggtgaca tcctgctcgt caacttcaat gtaagggtaa gtatcggagc gggactgtgg
     1141 gtcaaggagc agggcatcgc agaccacatt ggagcgcacg tgctcggcac ctttatgcac
     1201 gcggagcaga ccgcgatagg aagaccgccc gccgcccttg ctgatggact tggaagtgat
     1261 tttgctgctg gtgtggggtg caaagtggac catcttgccg ccagcatcct gctgctgacc
     1321 agggccggca aaggccatgg agaggatttc gccatgagcg ccggggccca tcagatagca
     1381 ggaggggtat ttcatcgtca ctttggaacc caggttgcaa tccacccatt ccatggtgcc
     1441 gttttcttcc acgcgggcgc gctgggtaac caaattgtaa acgttgttcg accagttctg
     1501 gatggtggta tagcggacgc gggcgccttt gcgcaccacg atttcaatta cgccactgtg
     1561 gaaagaagcc tcgctgtact gcggagcggt gcagccttcg acataatgga cctgggcgcc
     1621 ttcatcggca atgatgaggg agcgctcaaa ctgaccgatt gaggccatat tcaagcggaa
     1681 gtaagcctgc aggggcaggt ccactttgac gcccgggggg acatagacaa aggagccccc
     1741 agaccagacc gcgctgttga gggcggcgaa cttattatcc tcaatgggaa caacggtgcc
     1801 aaagtactgg cggaaaaggt cgggatgctg gcgcaggccg tcttcgatgc tcaagaagat
     1861 gacgccctgc ttttcaaggt gctcttgaat gctgtgatag accatttccg attcatactg
     1921 ggcgccaaca cctgccagga atttgcgctc agcctcgggg atgcccaggc gatcaaaggt
     1981 gtttttgatg gtatcgggca catcatccca ggtacgcccc tgagaatcgg tgggacgcac
     2041 gtagtaatag atatcatcca ggttcaggcc ggaaatatcg gccccccagg atggcatggg
     2101 gcgcgcatcg tagtgttcca gcgcccgcaa gcgaaattca agcatccatt cgggttcgcc
     2161 cttcagggca gaaatctggc ggacgacatc gctgtcgagg cctctgcgcg ttttgaagac
     2221 cgaagtgtct ggatcactaa atccgtactg gtaatcggtt aattcgttta atatatctga
     2281 ctgcggctta tcgctcattg ctacctccag tatttggggc cagactacgc agcaggtgat
     2341 tcattggcca atttttcacg aacccagtcg taaccgtgtt cttcaagatg cagggccagg
     2401 tcggcgccgc cggattcaac aatccgaccg cccatcatga c
//
LOCUS       DAOPVS010000238         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_45657_length_2435_cov_56.073109, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000238 DAOPVS010000000
VERSION     DAOPVS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45657_length_2435_cov_56.073109"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1790
                     /locus_tag="PKL11_11100"
     CDS             63..1790
                     /locus_tag="PKL11_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNZ14190.1"
                     /translation="MYLTPDNSIRQTQSRDLVEIHLPDGRTFEGPRGLPVGIFLRALP
                     EWDNPPIVGAVINDELRELTYPIDIESRVRPVTMAEADGARIYRRSITFLLEAAFEEL
                     FPNDDLIVDHSVASGGFFCWLDGRAPLTTAELAHLENRMREIVAADLPLERHTVPLEE
                     AIAYFQSKDYDDKVRLLKYRQRKELVLYRLLHHQDYHHGYMVPSTGFLRWFTLTLLGT
                     GFVLQYPRRNAPTELLPMPEYPKLLNTFRQYEQWLTRLGIASVGALNDSIVAGRAREI
                     ILVAEALHEQQISDIAQQIVERVNQARIVLIAGPSSSGKTTFSKRLAVQLLSQGLSPF
                     PLEMDNYFVDRDRTPRDEDGRFDFEALEALDTVRLQSDLQHLLAGEKVQLPHYNFHTG
                     MSEPGEWVQLAPDQLVILEGIHGLNPRLLPDIPSAQTFRIYVSCLTQLNLDRYNRIST
                     TDTRLIRRIVRDARERGYTAKDTIGRWDMVRRGEKRHIFPYQEYADAMFNSAQVVELS
                     VLRPLAEPLLRQVPFGHPEYIEAKRLLSFLQWFQPIEADLIPGNSILQEFLGGSSLKA
                     FKLWEHNNG"
     gene            complement(1869..>2435)
                     /locus_tag="PKL11_11105"
     CDS             complement(1869..>2435)
                     /locus_tag="PKL11_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ14191.1"
                     /translation="LTEDGVETIFATNHLGVFLMTRLLLDVLKSSAPSRIITVASKGL
                     TLYPALEIEFDNLNGEHKFSTQHAYYHSKQAQVMFTYDLAERLKGSGVTVNCVRVTNV
                     AIPDERLTHLPKWLLKIYQLKRSMAITPEQQAETYVYLAADPAVQDITGGYWDEHNQM
                     VRSNAKSYNREMWRRLWDVSSKLTGLEQ"
BASE COUNT          488 a          828 c          585 g          534 t
ORIGIN      
        1 gtcttttcct gtggtatact atccgtcccg ttggacggga taactcactt taagggatgg
       61 ctatgtacct tacgccagat aattctattc gacaaaccca atcgcgcgac ctggtagaaa
      121 ttcatctgcc cgatggccgc acctttgaag gcccgcgcgg cctgcctgtg ggcatcttcc
      181 tgcgcgccct gcccgaatgg gataacccgc ccattgtcgg cgccgtcatc aatgacgaac
      241 tgcgcgaact gacctacccc atcgatatcg aatcccgcgt tcgccccgtc accatggccg
      301 aagccgatgg cgcccgcatt taccgccgct ccatcacctt cctgctcgaa gccgcctttg
      361 aagagctgtt ccccaatgac gacctgattg tggatcactc agtcgcctcg ggcggcttct
      421 tctgctggct cgatggccgc gctccgctga ccaccgccga actcgcccac ctcgaaaacc
      481 gcatgcgcga aatcgttgcc gccgacctgc ccctggagcg ccacaccgtc ccactggaag
      541 aggccatcgc ctacttccaa tccaaggact atgatgataa ggttcgcctg ctcaaatacc
      601 gccagcgtaa agaactggtg ctctaccgcc tgcttcatca tcaggattac caccacggct
      661 acatggtgcc ttccaccggc ttcctgcgct ggttcaccct gacgctgctc ggtacgggct
      721 ttgtactgca gtacccccgc cgcaacgcgc cgactgaact gctccccatg cccgaatatc
      781 ccaagctgct caacaccttc cgccaatatg agcagtggct cacccgcctg ggcatcgcca
      841 gcgttggcgc cctcaacgat tccatcgtcg ccggccgcgc ccgtgagatc atcctggtgg
      901 ctgaggccct ccacgaacag caaatttcag acatcgccca gcaaatcgtt gaacgcgtca
      961 atcaggcccg catagtcctg atcgccggac cgtcctcctc cggcaaaacc actttctcca
     1021 agcgtctggc tgtgcagctc ctttcccagg ggctttcccc cttcccgctc gagatggata
     1081 actattttgt agatcgagac cgcaccccgc gagatgaaga tggtcgtttt gacttcgaag
     1141 cgctcgaagc cctggatacc gtccgcctgc agtccgatct ccagcacctg cttgctggtg
     1201 agaaggttca actcccgcat tacaacttcc acaccggcat gagcgaaccc ggcgaatggg
     1261 tgcagttggc ccctgaccaa ctggtgatcc tcgaaggcat tcacggcctc aacccccgcc
     1321 tgctgcccga tatcccatcc gcccagactt tccgcatcta cgtctcctgc cttacgcagc
     1381 taaacctgga tcgctacaac cgcatctcca ccaccgatac ccgcctgatc cgccgcattg
     1441 tgcgcgatgc ccgtgagcgc ggctacaccg ccaaagatac cattggccgc tgggatatgg
     1501 ttcgccgcgg tgaaaaacgc cacatcttcc cctaccagga atatgccgat gccatgttca
     1561 actcggccca ggtcgttgag ctttcggtcc tgcgtcccct tgccgaaccg ctgctccgcc
     1621 aggtcccctt tggtcacccg gaatacatcg aagccaagcg cctgctgagc ttcttgcagt
     1681 ggttccaacc catcgaagca gatctcatcc ccggcaactc gatcctgcaa gaattccttg
     1741 gcggctccag cctcaaggcc ttcaaactct gggaacacaa caacggctaa cctgccctcg
     1801 acgggtagat aatgcaacca caccgccctg gcaatttatt cgccagggcg gtttttgtct
     1861 ggctggactt actgctccag cccggtcagt ttgctgctca catcccacag ccgccgccac
     1921 atctctcgat tgtatgattt ggcattcgat cgcaccattt gattatgttc atcccagtac
     1981 ccaccggtga tatcctgaac ggccggatca gccgccagat aaacataagt ctcggcctgc
     2041 tgttccgggg tgatggccat gctccgcttg agctgataga ttttcagcag ccatttgggc
     2101 agatgggtca gccgttcgtc tggaatagcc acattcgtca cgcgcacaca gttgaccgtt
     2161 acgccgctgc ctttcagccg ttcagccagg tcataggtaa acatcacctg cgcctgtttg
     2221 gaatgataat aagcgtgctg cgtgctgaac ttgtgctcgc cgttcaggtt gtcaaattca
     2281 atctctagag cagggtacag ggtcaatccc ttggatgcca cagtgataat tcggctgggc
     2341 gcgctgctct tgagcacatc cagcagcagc cgggtcatca aaaatacgcc caggtggttg
     2401 gtcgcaaaga tggtttcaac cccatcctcg gtcaa
//
LOCUS       DAOPVS010000239         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_46857_length_2392_cov_44.917415, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000239 DAOPVS010000000
VERSION     DAOPVS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46857_length_2392_cov_44.917415"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKL11_11110"
     CDS             complement(<1..670)
                     /locus_tag="PKL11_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNZ14192.1"
                     /translation="MTKRILNWDGCTNARDLGGLHTLDGRQTRWQAVVRSDTPSRLTA
                     AGWSALYAYGIRTIITLRTHGQTEPELNFSPPQPGLAIVPVEIEDITDQAFLQQWAVT
                     ELWSTPLYYKDALQRWPDRHAAAISAIAQAQPGGVLFHCARGNDRTGIIALLLLTLAG
                     VTPDEIIADYQLSPDPCRDELLARQQSSVQAALLGALAGLNVTDYLLQGGASQADLSA
                     LRQRL"
     gene            complement(891..1187)
                     /locus_tag="PKL11_11115"
     CDS             complement(891..1187)
                     /locus_tag="PKL11_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ14193.1"
                     /translation="MKAELFAELVASVHEGAAILKDERPAARTFNLGPMDIKNIREGF
                     NLTQEQFAALLGISVRTLHNWEQGRRVPEGPAMVLLRVAEKHPEAVLDVVRQIS"
     gene            complement(1184..1501)
                     /locus_tag="PKL11_11120"
     CDS             complement(1184..1501)
                     /locus_tag="PKL11_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ14194.1"
                     /translation="MIIVETSIFTRQVLALLSDEEYRQLQAALLLKPDLGVVIPGGGG
                     LRKMRWALPGRGKRGGARVIYYWAVVQDKILMLVIYAKNEQDDLTPDQIKILRQIITE
                     EYP"
     gene            complement(1582..2280)
                     /locus_tag="PKL11_11125"
     CDS             complement(1582..2280)
                     /locus_tag="PKL11_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNZ14195.1"
                     /translation="MAINKTEKPGCLSKIFPFLRQRPSAVDSPIPQSVPKELPYRLRD
                     DFLSPAEFSFYKVLTNLLGTHFVVQSKVRLADIFYVARPNENRAFFNQISVKHLDFLV
                     CDPVTMQPLLGIELDDSSHQRSDRQERDDFVVEVFKVAKLPLLQIPVRREYNQREIAA
                     LVVPILKEKLQSAAAPLSETPTQSAGVPVCPKCGIPMILRTVSQGENQGKPFYGCRNY
                     PSCRQMKPFMPSKS"
BASE COUNT          570 a          611 c          657 g          554 t
ORIGIN      
        1 gaaggcgctg gcgaagggcg gataaatctg cctggctggc gccaccctgc agcagatagt
       61 ctgtaacatt cagaccggca agggcaccca gcaaggcagc ctgaacagag gactgctggc
      121 gcgccagcag ttcatcccgg caggggtcgg ggctcagttg ataatcggcg atgatctcgt
      181 ccggggtgac gccggcaagg gtcagcagca gcagggcaat gatgcctgta cggtcattcc
      241 cacgggcaca atggaaaagc acgccccccg gctgcgcctg cgcaatggcc gaaatggcgg
      301 ctgcgtgccg atcaggccag cgctgcagtg catctttgta atagagcggg gtagaccaga
      361 gttctgtgac cgcccactgc tgcaaaaacg cttgatcggt aatgtcttca atttcaaccg
      421 gcacgatggc aaggcctggc tgcggcgggg agaagttgag ttcgggctct gtctgaccat
      481 gggtgcgcag tgtgatgatt gtccggatgc cgtaggcata gagcgccgac cagcccgcgg
      541 ccgtgagcct ggatggggta tctgaacgga caacagcctg ccagcgcgtc tggcggccat
      601 ccagagtatg cagaccaccc aggtcgcgcg cattggtgca gccatcccag tttagaatcc
      661 gtttagtcat ggatcggctt ctcctatacc gggtatatca tcatcaggct gtggggaggg
      721 cgcgtggaat gcgccagata tttttattct acatcagatt gaatcttggg cagggatttt
      781 tgagccttaa gttaaaccct gactggcaca aagggttaaa cagccaaccg cccgggtccg
      841 ggcggttgag gagagggtag gtgtcgttac ccggtttgcc agagctcacg tcaggagatt
      901 tggcgcacaa catccagcac ggcttcggga tgtttttcag ctacacgcag caagaccatg
      961 gcaggacctt ctgggacgcg ccgcccttgc tcccagttat gcagggtgcg cacgctgata
     1021 cccagcagcg cggcaaattg ttcctgggtc agattaaagc cttcgcgaat atttttgata
     1081 tccatagggc cgagattgaa agtccgcgct gccgggcgct catccttcag aattgcggcg
     1141 ccctcgtgta cactggcaac cagttcggca aaaagttcag ctttcatggg tactcctctg
     1201 tgatgatttg acgcagaatc ttgatctgat cgggcgttaa atcatcctgt tcattcttag
     1261 catagatcac caacattagt atcttgtctt gcactacagc ccaataataa atgactcgag
     1321 cgccgccacg cttgccgcgt ccgggcaaag cccagcgcat ttttcgcaag ccaccaccgc
     1381 caggtataac gactcccaaa tctggtttga gaagcaaagc agcttgcagt tggcgatact
     1441 cttcatcaga aagcagtgcc aaaacctggc gagtaaagat tgaggtttcg acgatgatca
     1501 caccgatttc ctccctgtta ttatacgtca ttggcgtagt tatgtcaagt cgtcttcaaa
     1561 aactaattgc atcggcattt cttatgattt tgaaggcatg aagggtttca tctgccggca
     1621 gctcggatag ttacggcagc cgtaaaatgg cttgccctga ttttcgccct gcgaaaccgt
     1681 gcgcagaatc ataggaatgc cgcatttagg gcaaacaggc acaccagcag attgcgttgg
     1741 agtctctgac aacggagcag cagccgactg cagcttctct ttcaaaatag gcacaaccag
     1801 cgcggcaatt tctcgttgat tgtattctcg tcggacgggt atctgtaaaa gtggtaattt
     1861 tgcgaccttg aaaacctcaa ccacgaagtc atcgcgttcc tgacgatcac tgcgttgatg
     1921 gcttgagtcg tccagttcga tgcccaagag tggttgcata gttacgggat cgcagaccag
     1981 aaaatcaagg tgcttgacag aaatctgatt aaaaaatgcc cggttttcgt ttgggcgggc
     2041 aacatagaat atatctgcca gtctgacttt ggattgcacc acaaagtgcg taccgagaag
     2101 attggtcagc accttgtaaa aagagaactc tgccggggaa agaaaatcat cccgcaaacg
     2161 atatggcaat tcttttggca cactttgcgg gattgggcta tccacagcgg aaggccgttg
     2221 ccttagaaat gggaaaattt tactgagaca accaggtttt tctgttttgt taattgccat
     2281 atggcgtcac ctccccaaac atatttacag tttcagcgtc aagtaattat acccattatt
     2341 aaaccccaaa cgccctcgag gtgagggcgt tcttctggcg gggagggcgg ga
//
LOCUS       DAOPVS010000240         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_47237_length_2378_cov_53.288420, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000240 DAOPVS010000000
VERSION     DAOPVS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47237_length_2378_cov_53.288420"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..520
                     /locus_tag="PKL11_11130"
     CDS             80..520
                     /locus_tag="PKL11_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14196.1"
                     /translation="MTITPTRFLTLSEASRRYGIDWKQLQQLIATGTIRAAEIRGEVI
                     VNESEVRAQIQRREDLPEYRQFGHLSGVAIWIGEASRKYNVPHPTLSRWKDKGLIKIL
                     GQESNKILLDEQDVAYCAYIYHQDGGQGKRIFADDGRPYINKPR"
     gene            1104..1478
                     /locus_tag="PKL11_11135"
     CDS             1104..1478
                     /locus_tag="PKL11_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ14197.1"
                     /translation="MINLQVIGNLGKDPELRYLPDGQPVARFSIAANRQWKDAAGKKY
                     SETLWVEVSAFGKLAEVCTQHLKKGRQVFVTGRPSLHTYQSKTGEPIASLRLTAASIE
                     FLGGKPDSAEPDAEDADAEIPF"
     gene            1481..1714
                     /locus_tag="PKL11_11140"
     CDS             1481..1714
                     /locus_tag="PKL11_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14198.1"
                     /translation="MARGSPYAWKVVQDPTRVGYDPGLFYGHLFRLSDIQVTDCWPEG
                     TVFRHTQTGDEIEVLGGRSVKKEVRSCAEVLQA"
     gene            1881..2159
                     /locus_tag="PKL11_11145"
     CDS             1881..2159
                     /locus_tag="PKL11_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14199.1"
                     /translation="MTTTIRFNQYRCQAVGLSIVQARCLHLAETQPTDAEMRQAFAGL
                     AQALAKCGLMELAAGRWRLTDAGRSKLEVCRSGARPAPRPDLTGSAAQ"
     gene            2156..>2378
                     /locus_tag="PKL11_11150"
     CDS             2156..>2378
                     /locus_tag="PKL11_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNZ14200.1"
                     /translation="MTAKIITIATHKGGVGKTTSAVNLAHGLALLGKQTLVVDADPQA
                     HAGTFLACSPAPDMFYLLMSGLVAMPSGND"
BASE COUNT          518 a          694 c          698 g          468 t
ORIGIN      
        1 cagagcgggc ggctgttaac cgctaggttc caggttcgag tcctggtcgg ggagcctttt
       61 ccttataagg ccaaatgaga tgacgataac cccgactcgc tttctcacac tttctgaagc
      121 ctctcgcagg tacggcattg actggaagca gttgcagcag ttgattgcga ctggtacaat
      181 tcgcgccgcc gaaattcgtg gtgaagtaat tgtgaacgag tccgaagtgc gcgcccagat
      241 tcaacgccgt gaagacctgc cggaataccg gcagtttggg catttaagcg gcgtggccat
      301 ctggatcggc gaagcatccc gcaagtacaa cgtcccacac ccaaccctct cacgctggaa
      361 ggacaagggg ctgatcaaga tcctgggaca ggaatccaac aagattctgc tggacgagca
      421 ggatgtggcc tactgcgctt atatctacca tcaggatggg ggacagggaa agcgcatttt
      481 tgccgatgac ggcaggccgt acatcaacaa accccgctaa agaaccgaaa taaggctggc
      541 agcgaccagc cttatttttt gcgccgaggt taacaaattg gcaaacctgt acggattttc
      601 gccgcgcgcc atcgaacata ttgacaaact tgtacggatt atgctatact ccgcctagca
      661 agtcagacgt cttctcgaca atttcccata ccccccattt ccctgtgggt gatgatcaga
      721 cagggtaaaa gcaagcggca gtcctttgct tgctgattca atctggcgaa agccgctcgt
      781 cgataacaac tctgcaagat gaacgcaggg ctgttgtttt cacgcacctt gacacagacc
      841 gtacctggga gcccaggcgg acagtcggat gcccaatcca atgggtccct tcatccggct
      901 gcggaggttc aaatccttca ctcccacctg gcaaatctct ggaggctgct gcctcaccag
      961 cacagagagg aagcccgatg accctgctac catcgggaaa cagccagggt gacagatcgg
     1021 agagacgatc aacccaggca aggcgggcag ctaactgctg ctgattcacc ggccgttggc
     1081 cggaaaacat tttcggagga cacatgatca acttgcaagt catcggtaat ctgggtaagg
     1141 atccagaact gcgctatctg ccagatggac agcccgtggc gcgtttttca attgctgcca
     1201 accgccagtg gaaagatgcc gctggcaaaa aatattccga aacgctctgg gtcgaggttt
     1261 cggcgtttgg caagctggct gaggtttgca cccagcattt gaagaagggc cgccaggtct
     1321 ttgtgaccgg ccgtccttcg ctccatacct atcagtccaa aaccggcgaa ccgattgcca
     1381 gtctccggct cacggcggcc agcattgagt ttctgggcgg caagcccgat tctgcagagc
     1441 cggacgctga agatgccgat gcggagatcc cgttctagcc atggccaggg gcagtcccta
     1501 cgcctggaag gttgtgcaag atcccacccg ggtggggtat gaccccggcc tgttttacgg
     1561 gcatctgttc cgcctgtctg atattcaggt gacagactgc tggccggagg gaacggtgtt
     1621 tcggcatacg caaaccggcg acgagatcga ggtgctggga ggcaggtctg tcaaaaaaga
     1681 ggtgcgctcg tgcgccgaag ttctgcaggc ctgatcattc cagaggcttt ggcctgccgc
     1741 gtctcagcac aggccgcccg ggttactaat cgaaactgct ggatgttgtg atgggttttg
     1801 cgctctgcct gccgcaggca ccggcaggca gaccagagga cccatcctct aattcacatc
     1861 aggagagagg ctttgacatc atgaccacca ccatccgctt caaccagtac cgctgccagg
     1921 cggttggcct gagtattgtg caggctcgct gcctgcacct ggcagaaacc cagccgacag
     1981 atgccgagat gcgccaggcg tttgctggcc tggcacaggc cctggctaag tgcggcctga
     2041 tggagctggc tgcgggtcgg tggcggctga cagacgccgg acgctccaaa ctggaagtct
     2101 gccggagcgg cgcccgcccg gccccgcgcc cagacctgac cgggagcgcg gcgcaatgac
     2161 cgcgaaaatc atcaccattg ccacgcacaa gggcggggtc ggcaagacga cctccgccgt
     2221 caacctggcg cacggactgg ccctgctggg caagcagacc ctggtggtgg atgccgatcc
     2281 gcaggcccat gcggggacct tcctggcctg cagtccggcg ccggacatgt tttacctgct
     2341 gatgtccggg ctggttgcga tgcccagcgg caacgacc
//
LOCUS       DAOPVS010000241         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_47388_length_2373_cov_46.081536, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000241 DAOPVS010000000
VERSION     DAOPVS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47388_length_2373_cov_46.081536"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1308)
                     /locus_tag="PKL11_11155"
     CDS             complement(70..1308)
                     /locus_tag="PKL11_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ14201.1"
                     /translation="MKIFKFGQPALLFLLATIIDGTIYSAWSLFFNFFILSRGYDQTF
                     LGMVNAAPSFAALVLGIPLGMLSDRLGRRRAMLLGLLIYSLGALGQLLAPGRSLLLLA
                     SLTAGTGNTLYYLSQAPFMMKASTPQNRAMLFSLNFGLITLAGALGSILAGSLPPMLA
                     AVLQVAEKHVSVYQTILFGAVAIGSLSLIPIFLIREPADPQPTAFGVQPEKQSIFKVL
                     LRPVVLKLFIPNILIGLGAAILIPYQNIFFDGRFGTSASALGQLFGVSALLTGIGSVI
                     GPILERPLGGKIRTVVLTQAGSLAFLLLMGFSPYFSLAVVGFLVRAMLMNMASPLYTA
                     FSMEQIEPHDQGAVNSLLNISWTAGWAVGPIISGVVQQRWGFTPLFVATAVLYSLAIL
                     LTWWFFVRRAPVLTTQPTQA"
     gene            complement(1320..1928)
                     /locus_tag="PKL11_11160"
     CDS             complement(1320..1928)
                     /locus_tag="PKL11_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNZ14202.1"
                     /translation="MTIRALIFDMGGVLLRTEDHQPRLKLSGDLGIDYDGLMKLAFGG
                     ESARRATIGEITEEEHWEIVRQQIKKSPAEMAAWCEQFWTADKHDTELIQFIGNLRSR
                     LRTGLLSNAWSGARSAITRRFPGTLDVFDVTIFSAEVGMAKPDAAIYHHILKLMDIEP
                     HEAIFVDDFIENIQAARQLGMVGVHFHDSLQARQDVIAALNH"
     gene            complement(2092..>2373)
                     /locus_tag="PKL11_11165"
     CDS             complement(2092..>2373)
                     /locus_tag="PKL11_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ14203.1"
                     /translation="STDEDAEMGWVQTLGVRRPWRKQGLGLALLHHAFGELYRRGRHA
                     VGLGVDASSLTGATRLYERAGMRVVRQFNSYAKVLRPGIELGTETVTVE"
BASE COUNT          585 a          645 c          723 g          420 t
ORIGIN      
        1 agcattcacc cggtcgggca catgggtatc ggagaccacg ccaatcaaca ggggggcagc
       61 gggcatcgtt caggcctggg tgggctgagt tgttagcacc ggggcccgcc ggacaaagaa
      121 ccaccaggtt agaaggatgg cgagactgta gagcacagcc gtggcaacga aaagcggcgt
      181 aaagccccaa cgctgctgca ccacgcccga aatgatgggg ccaaccgccc agcctgccgt
      241 ccacgagatg ttgagcagac tattgaccgc gccctgatcg tggggctcaa tttgctccat
      301 tgagaaggcc gtatagagcg gggaggccat attcattaac attgcgcgga caagaaagcc
      361 aacgaccgcc agggagaaat aaggggaaaa gcccatcagg agcaggaaag ccaggctgcc
      421 cgcctgagtg agaaccaccg tgcggatttt accgcccagg ggccgctcga ggatggggcc
      481 aatgacactg ccaatgccgg tgaggagggc tgagacgcca aagagctgtc ccagcgcaga
      541 tgccgaggta ccgaaacgac catcaaaaaa gatattctga tacgggatca gaatcgccgc
      601 accaaggcca atcaggatgt tagggatgaa gagcttgagt acaaccggac gcagcaggac
      661 tttgaagatg gattgctttt caggctggac gccaaaggca gtgggctgag ggtcggctgg
      721 ttcgcgaatc aagaagatgg ggatcagcga gagcgagcca atggccacag cgccgaagag
      781 gatggtctga tagacggaga cgtgcttctc agctacctgc aagacagccg ccagcattgg
      841 cggcagtgag cccgccagaa tactgcccag cgcgccggcc aacgtaatca gaccgaaatt
      901 caggctgaag agcatggccc ggttctgcgg ggtagaggcc ttcatcataa agggcgcctg
      961 gctcagataa tacagcgtgt tcccagtacc ggctgtcagg ctggctaaca gcagcaagga
     1021 ccgaccaggg gcgagtagct gcccaagagc ccccagtgag taaatcagca ggcccagcag
     1081 catggcccgg cggcggccca gccggtcaga gagcatgccg aggggaatgc ccaataccag
     1141 cgcagcaaac gaaggggcgg cgttcaccat ccccagaaag gtctgatcat agccgcggct
     1201 tagaatgaaa aaattgaaga acaacgacca ggcgctgtag atcgtgccat caataatggt
     1261 ggcaagcaaa aacaggagcg ccggttgtcc gaatttgaag attttcacac aacctcaaat
     1321 caatggttta atgcagcaat cacatcctgg cgagcctgca gcgaatcatg gaaatgaaca
     1381 cctaccatgc ccaactgccg ggcagcctgg atgttttcaa taaagtcatc cacaaagatc
     1441 gcttcatggg gttcaatatc catcagcttg aggatgtggt gataaatggc ggcatcgggt
     1501 ttggccatgc ccacttctgc tgagaagatg gtcacgtcaa agacatccag ggtgccgggg
     1561 aaacggcggg tgatggctga acgggcaccc gaccaggcgt tgctgagcag gccggtgcgg
     1621 agccgggagc gtaaattgcc aataaattga atcagttcag tgtcgtgctt gtcagccgtc
     1681 cagaactgct cacaccaggc ggccatttcg gccggagatt tcttgatctg ctggcggaca
     1741 atttcccagt gctcttcttc ggtaatttca ccgatggtag cccgccgggc tgattcgccg
     1801 ccaaaggcca gtttcatcag gccatcataa tcaattccca gatcgcctga tagtttgagg
     1861 cggggttggt ggtcttcggt gcgcaggagg acaccgccca tgtcaaagat aagtgcccgg
     1921 attgtcatgt ctttcgcatc ctcgttttct tagattaatg ctggcaagta taacacaggg
     1981 aatcggcagc ctatcaggcc cgcattccaa agaaagttgg atcatccggg tgaccccgcg
     2041 gctgtccgac aggcgggatt gactgcctgc cggacaggat gcggggaagt tttattcaac
     2101 ggttaccgtt tcagtgccca gttcgatgcc ggggcgcaga actttggcat agctgttgaa
     2161 ctggcgcacc acgcgcatgc cggcgcgttc gtagaggcgg gtggcgccag tcaggctgga
     2221 ggcatcaacc cccaggccaa ccgcatggcg gccgcggcgg tacaactcac cgaaggcatg
     2281 gtggagcagc gccagtccca gaccctgctt gcgccagggg cggcgcacac ccagagtctg
     2341 cacccagccc atttctgcat cttcatcagt aga
//
LOCUS       DAOPVS010000242         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_47640_length_2365_cov_48.493939, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000242 DAOPVS010000000
VERSION     DAOPVS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47640_length_2365_cov_48.493939"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..901)
                     /locus_tag="PKL11_11170"
     CDS             complement(101..901)
                     /locus_tag="PKL11_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ14204.1"
                     /translation="MLITSTSNPQIKQIRHLRDRKARQETGLFYMEGLRIVTEALQQG
                     LTLQQLIVAPELLVSTVGQQAALEQARRGAPVLEVSAEVFRSFSLKDGPQGIAAIAHQ
                     RWSKLNEEKPEPLDCWVALDSVADPGNLGTILRTHDSVGGQGVILLDQSTDPYDPTAL
                     RASMGAAFSQRLIRATFAEFITWAQHWNLPIVGAAGGSETDYHAYHYPTPLVLLMGSE
                     RQGLQPHHLSACSAVVAIPMQGRSDSLNLAVATAVILYEIYNQRRASA"
     gene            complement(904..1401)
                     /gene="ruvC"
                     /locus_tag="PKL11_11175"
     CDS             complement(904..1401)
                     /gene="ruvC"
                     /locus_tag="PKL11_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ14205.1"
                     /translation="MLALGIDPGTAITGYGLVRETADGLAMVDYGVITTPAGESLEKR
                     LLLLYQRLTEYLLLHRPESAAVEKLFFQRNVTTALAVGHARGVVLLALAQAGLSVAEY
                     TPLEVKQAVAGYGGADKHQMQEMTRALLGLSDIPRPDDAADALGIAICHLSTGRYHAL
                     LDQGS"
     gene            complement(1408..2160)
                     /locus_tag="PKL11_11180"
     CDS             complement(1408..2160)
                     /locus_tag="PKL11_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ14206.1"
                     /translation="MSGHSHWATIKRKKGAGDAKRGQLFTRLTREIVMAAREGGGDAD
                     GNARLRIAVEKARAQNMPKDNIERAIKRGTGDSKDGNIYEQIMYEGYGPNGVAVIIEC
                     VTENRNRTVADLRHLLSRSGGNLGESGSVAWQFKRVVSFTVAGTNDFDKVFEAALEGG
                     ADDVTQEAEDIEIVGPVEAFKTLSDALRKANIQIEEGGIRMIPNQEMELSVEQTIQAM
                     RFIEGVEDLDDVQNVYSNLLVTEEALAAMESA"
BASE COUNT          515 a          645 c          687 g          518 t
ORIGIN      
        1 cgcaaggtaa gcggaacaaa gacccgcagt cccatgccgc ccagtcgaat agtgagataa
       61 tcttccccgc cgcccatgac atggccttcc aggtaagcga tcatgcggat gccctcctct
      121 ggttgtagat ttcatacagg ataacagcag tggcaacagc taaattaagt gaatcgctgc
      181 ggccctgcat ggggatagcc accaccgctg agcaagccga cagatggtgc ggctgcaagc
      241 cctggcgttc gctgcccatg agcaagacca ggggggtggg gtagtgatag gcatgataat
      301 ccgtctcgga gccgccagca gcgccaacaa tcggcaggtt ccagtgctgc gcccaggtaa
      361 taaactcggc aaaggtggcg cggatcagcc gctggctgaa ggcggccccc attgacgcgc
      421 gcaaggcggt tggatcgtaa ggatccgtag attgatctaa taagatgacg ccctgcccac
      481 caactgaatc gtgagtgcgc aggatggttc ccaggttgcc ggggtcggca acggaatcaa
      541 gagcgaccca gcaatctaag ggctcgggct tttcttcatt tagtttactc cagcgttgat
      601 gagcaatggc agcaataccc tgcgggccat ctttgagcga gaaggagcgg aaaacctctg
      661 cgctgacttc cagcaccggg gcgccgcggc gagcctgctc aagcgcagct tgctgcccca
      721 cggtgctgac caggagctcg ggggcaacga ttaattgctg gagggtaagg ccttgctgga
      781 gcgcctcggt gacgatgcgc aaaccctcca tatagaacag accggtttcc tgacgcgctt
      841 tgcggtcgcg caggtggcga atttgtttaa tttgcgggtt ggatgtgctg gtgatcaaca
      901 tggtcaggat ccttgatcca gcaaggcatg atagcgcccg gttgagaggt gacagattgc
      961 gatgcccagc gcgtcggcgg catcatcggg gcgtgggatg tctgaaaggc ccagcaaggc
     1021 acgggtcatt tcctgcatct ggtgcttatc ggcaccaccg tagccggcaa cagcctgctt
     1081 gacctccaga ggggtgtatt cagccaccga caagcccgcc tgagccaggg ctagtaaaac
     1141 cacaccgcgg gcatggccca ctgccagggc agtggttaca ttccgttgaa aaaaaagctt
     1201 ctccacagcg gcactttcgg gccggtggag aagcaggtat tcagtcaagc gctgatagag
     1261 cagcaataaa cgtttttcca gggattcacc ggcgggcgtg gtaatgacac cataatccac
     1321 catagcaagg ccgtcggcgg tctcacggac caggccatag ccagtgatgg cggtgccggg
     1381 gtcaatcccc agtgccaaca tggctgttta ggcagactcc atggcagcga gggcttcttc
     1441 agtcacaagc aggttggaat agacgttctg aacatcatcg aggtcttcaa cgccttcaat
     1501 aaagcgcatg gcctggatgg tctgttccac gctcagttcc atttcttgat tggggatcat
     1561 gcggatgccg ccttcctcaa tctggatatt ggctttgcgc agggcgtcgg agagcgtttt
     1621 gaaggcctca accgggccaa caatttcaat gtcttcggct tcctgggtca catcgtcagc
     1681 gccaccttcg agggcagctt caaagacttt atcaaagtca ttggtgcctg ccacagtgaa
     1741 ggagaccacg cgcttgaact gccaggccac cgagccggac tcgccgaggt tgccgcccga
     1801 gcgggagagc aggtgccgga ggtcggccac tgtgcgattg cggttttcgg tcacgcattc
     1861 gatgattaca gccacgccgt tagggccata accttcgtac atgatctgtt cgtatatgtt
     1921 gccatctttg ctgtcgccgg tgccgcgctt gatggcgcgt tcgatgttat ctttcggcat
     1981 gttctgagcg cgagcttttt caacagcaat gcggagccgg gcgttgccgt ccgcgtcgcc
     2041 gccgccttcg cgcgcagcca taacgatttc acgggttaag cgggtgaaga gttgtccacg
     2101 ttttgcatca ccagcgccct tcttgcgttt gattgttgcc caatgggaat gaccagacat
     2161 tcttgccagc tccttaatca gaggaatata caaagattcg ccccattata ccactatcat
     2221 ctggttcgga cgaaattaaa ccaggaattt tttctggatt taaggctatc taaatgacgg
     2281 gccacaaacc caatgctact gtttgcaaac agtgaagtct ccgaggactg gcggcggctt
     2341 tgcatttttg taagtcatca ttttt
//
LOCUS       DAOPVS010000243         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_49045_length_2318_cov_60.696863, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000243 DAOPVS010000000
VERSION     DAOPVS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49045_length_2318_cov_60.696863"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..850)
                     /locus_tag="PKL11_11185"
     CDS             complement(200..850)
                     /locus_tag="PKL11_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14207.1"
                     /translation="MKNSKFALLTLAGLLFVLLIGGCIPIESVDTEITLQTRERWQMG
                     LDITVAPDSGMTVAQLENEMLSQFNTQTLQSQGIQVNSSAEEDKDGRIIIQLIFEGQG
                     LDLLNQTLSGGSTILREDRQGGRDVIVFEMAPVMQFGSMDFQLTLKGARVISSNGVEQ
                     GGNQVTWTNPTQSMQAVLAKPALFDTLPVILLIVGGILAFVGLIGLLTTLGSRRVG"
     gene            complement(856..1662)
                     /locus_tag="PKL11_11190"
     CDS             complement(856..1662)
                     /locus_tag="PKL11_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HNZ14208.1"
                     /translation="MPKEFEEEPIEQPQPRPEVPPLSRRGRQSIQGVSPAPEAAADDA
                     ALPDAKVPANDFKNPPEPVTEDLKPERPAYKNLDKTELSGLVQAASENPADGSLERNN
                     SQLYRNMEVFYGPHPAGYDRHHLIPVDVAQNHPAMQTAARLGYDINNPNNAIYLPDTA
                     SLAAQTGEPLHTKETHSYDRVVQAELDDVWTRYEHQRVKTGSSLAMADYLKASDQQTA
                     FLQDIQHVENSIRQKLVSRDLFITESDPNYKPPTDYKKLDKKQPENRIKV"
     gene            complement(1678..>2318)
                     /locus_tag="PKL11_11195"
     CDS             complement(1678..>2318)
                     /locus_tag="PKL11_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNZ14209.1"
                     /translation="VVRMIVAGIHGGYEWNTVDLANQLAMDFREDTLIVPSDTTLYIL
                     ANLNPDGYANYWGQYAGRPNANGVDLNRNWDSSWQSDWPLDGCFSAIKLNGGTVPFSE
                     PETQALAQFLLDHSVDALISYHSQMAAIYATGDATLDPAADNLAQTLAAVSGYQYPPP
                     WSACVYTGQLVDWAVDQGIAAVTVELTNHDDTDLEINRRLVTAFLAWRRPAE"
BASE COUNT          538 a          589 c          612 g          579 t
ORIGIN      
        1 ccggtctggt caccggactc aacgcagctt gcttatgcta gctgggatgg caagatcttt
       61 atcgtcagct ttgacgggag tgagattcgt aaaattgagc cattccatga tgatgccgcg
      121 ctgctggggt gggtcgtcat aaaaggcgga gaggttgtca gataggcgcc agggggccgc
      181 gctaaagttc atcatctcat tacccgaccc ggcggctgcc cagggtagtg agaagcccga
      241 tcaagccaac gaatgctaag atgcctccaa caatcagaag aatgacgggc agtgtgtcga
      301 aaagcgcagg tttagccagg acagcctgca tggattgtgt gggattggtc caggtaactt
      361 gattgccacc ctgctccacg ccgttgctgg aaataacccg ggcacctttc agcgtcaatt
      421 gaaagtccat gctcccgaat tgcatcaccg gtgccatttc aaacacaatg acatcgcgtc
      481 cgccttgccg atcttcacgc aagatggtcg aaccaccgga aagagtctga ttcaataaat
      541 ccaggccctg accttcaaag atcaattgga tgataatccg gccatcttta tcttcctcgg
      601 cgcttgaatt tacctgaatg ccctgagatt gcagggtttg cgtattaaac tgactcagca
      661 tctcgttttc aagttgagcc acggtcatcc cagaatcggg ggccacagtg atatcgagac
      721 ccatctgcca gcgctcccga gtttgaaggg tgatctctgt gtcaaccgat tcaattggaa
      781 tgcagccgcc gatcagaagc acgaacagca agccagccag ggtcagcaaa gcgaatttgg
      841 agtttttcat gttggctaca cctttattct gttctcgggt tgttttttgt caagtttctt
      901 gtagtcggtg ggcggtttat agttcggatc tgattcggtg ataaagagat cacgcgaaac
      961 cagtttctgc cggatggaat tctcgacgtg ctggatatcc tgtaaaaaag cggtctgttg
     1021 gtcactggct ttcagataat ctgccattgc aagagagctg ccagttttga cccgctgatg
     1081 ctcgtaacgc gtccagacat catccaactc tgcctggact acccgatcat aagagtgggt
     1141 ctcttttgta tgcagcggtt cccccgtctg cgcagccaga gaagccgtat cgggaaggta
     1201 aattgcgtta ttgggattat taatatcata gcccagccgg gctgccgttt gcattgccgg
     1261 gtgattttgg gccacatcca ccgggatcag atgatgacgg tcataacctg ccggatgggg
     1321 gccgtaaaaa acctccatat tgcgataaag ttgggaatta ttgcgttcta gagatccatc
     1381 tgcagggttt tcgcttgcag cttgaacaag gccagataat tcggttttat ccaaattttt
     1441 gtaggctggc cgttctggtt taagatcttc agttactggt tcagggggat tcttaaaatc
     1501 atttgcaggg actttggcgt ctggcagagc agcatcatca gcagccgcct ccggggcagg
     1561 agatacgcct tgaatacttt ggcgcccccg acgactgagt ggaggcactt ctggcctggg
     1621 ttgaggttgt tcaattggtt cttcttcaaa ttctttcggc attgctatac ccaattttta
     1681 ttcggctggc cggcgccatg ccagaaaggc cgtcaccaga cggcggttaa tctcaagatc
     1741 cgtatcatca tggttcgtca attcaactgt tactgcggca ataccctgat ctaccgccca
     1801 atccaccaac tgcccggtat agacacaggc tgaccagggc ggcggatact gataaccact
     1861 gactgcggcc agggtctggg ccaggttatc tgcggcaggg tcgagggttg catctcctgt
     1921 ggcataaatc gccgccatct gactatggta gctaatcaac gcatctacag agtgatcaag
     1981 caagaattga gccagggcct gggtctcggg ttctgagaag ggcaccgttc cgccattcag
     2041 tttgatggcg ctgaagcatc catccaacgg ccaatcggac tgccagctgg aatcccagtt
     2101 gcggtttaaa tccacaccat tggcattcgg gcgtccggca tactgtcccc aataattggc
     2161 atagccgtcc ggattaagat tggcaaggat atagagcgtg gtatcggagg gaacaattaa
     2221 tgtgtcctca cgaaaatcca tggccagttg attggccagg tccaccgtgt tccactcata
     2281 accgccgtga atacccgcca caatcatccg caccacgg
//
LOCUS       DAOPVS010000244         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_49303_length_2309_cov_13.341171, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000244 DAOPVS010000000
VERSION     DAOPVS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49303_length_2309_cov_13.341171"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..318
                     /locus_tag="PKL11_11200"
     CDS             58..318
                     /locus_tag="PKL11_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNZ14210.1"
                     /translation="MTLTISPKGWVVIPAELRKKYHLHPGQVVQVVDYGGVLALVPAA
                     ADPICHAAGLLHTPEDSLTEALLAERAAEKAREDKLDLSDAR"
     gene            308..724
                     /locus_tag="PKL11_11205"
     CDS             308..724
                     /locus_tag="PKL11_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNZ14211.1"
                     /translation="MPAEKPGYVLDSFALLAHLQKEGGQPRMVELLQQAQRGQCRLWM
                     SLINLGEVCYLVERRRGLRAVQLMLADVQSLPIEMAPANQEAVLAAAHLNAGFPISYA
                     DAFAAATAQMHHAILLTGDPEFKALGELLQVEWLAG"
     gene            complement(889..1551)
                     /locus_tag="PKL11_11210"
     CDS             complement(889..1551)
                     /locus_tag="PKL11_11210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ14212.1"
                     /translation="MTVPILLYHHINDEQDNRYTVSTEDFAAQMQQLHALGYQSITIA
                     DLSHVITQGGELPERPIIITFDDGNLDIYQKAMPIMQQYGYVGVMYIVANRLESDDFL
                     HASELQNMVALGWEIGNHSMTHSDLTAEGVDLYFEMVESRVRIERATGSDVTSFAYPF
                     GVINETVVQKIYEYGYFSAVGLGTGWEQSFNNLYYLTRREVMGEASVEEFTALLPWVQ
                     RP"
BASE COUNT          515 a          598 c          633 g          563 t
ORIGIN      
        1 ccaatttttc cagaacgcgt tatgctattt ctgaaaaagt ataacgcgga ggcgatcatg
       61 actcttacta tctcccccaa aggctgggtc gtcattccag ccgaattgcg caagaaatac
      121 cacctgcatc ccggccaggt tgtccaggtg gtggattatg gcggcgtgct ggccctggtg
      181 ccggccgccg ccgacccaat ctgccatgct gccgggctgt tgcacacacc agaagactcg
      241 ctgaccgaag cgttgctggc ggaacgcgcc gctgaaaagg cccgtgaaga taaactggat
      301 ttgtccgatg cccgctgaaa aacctggcta cgtgctggac agttttgcgc tgctggctca
      361 cttgcaaaaa gagggcggcc agcccaggat ggtggagctc ttgcagcagg cccagcgcgg
      421 ccaatgccgc ctgtggatgt cgctgatcaa ccttggcgag gtatgctacc tcgttgagcg
      481 ccggcgcggc ttgcgcgcgg ttcaacttat gctggcagat gttcagagcc tgccgattga
      541 aatggcgccc gcgaaccagg aagcggtgct ggcggctgcg catctcaatg ccggttttcc
      601 catttcgtac gccgatgctt tcgccgccgc gacagcccag atgcatcacg ccatcctgct
      661 gaccggcgac ccagagttca aagcgctggg cgaactgctt caggttgaat ggctggctgg
      721 ctaatactgc aggtatggga gatacagaat ctaatttgat aacagtgccg ctgggggtaa
      781 cctattgttt tgatgattcc catgcatcgt ctgtgaccag atcggtctga ttttactacg
      841 gaacacctct ggaatatcgt gtgacctgac ggctgtccat ttgggagttt atggccgctg
      901 cacccatgga agcagcgctg taaattcctc cactgaagcc tcacccataa cctcccgccg
      961 ggtcaggtaa tataagttgt tgaaggactg ttcccatccc gttcccaaac caacggcaga
     1021 aaaataaccg tattcataga ttttttgaac cacggtttca ttgatcaccc caaacgggta
     1081 ggcaaatgag gtgacatcac tacccgttgc ccgttcaatt cgaacgcgag attccaccat
     1141 ttcaaaatat aaatccaccc cttcagcagt caggtcgctg tgggtcatgc tatgattgcc
     1201 gatttcccat cccagggcaa ccatattctg taattctgat gcatgcaaaa aatcatcaga
     1261 ttccaatcgg ttggcgacaa tatacatgac cccaacgtag ccgtactgct gcatgatggg
     1321 catcgctttt tgatagatat ccagattccc gtcatcaaat gtgatgatga tgggcctttc
     1381 aggcagttcg cccccctggg tgatgacgtg cgataaatca gcgatggtga tgctctggta
     1441 tcccagggcg tgcaattgtt gcatttgggc tgcgaaatcc tctgtactga cggtataacg
     1501 attgtcttgt tcatcattaa tatggtgata aagtaaaatc ggcacggtca ctggcccagg
     1561 gcgatatgtt gcccaggtgg cagtgggcgt tggggtcgcg gtcggcgttg ctgtcggagt
     1621 cagggtggcc gtcggggtca gggtcggcag cggggtgctg gttgatgtgg ccgtcgcggt
     1681 cagggtcaac gttggtgttg gcgtttctgt ggctggtccg gccaggactg actgcagcgt
     1741 gcacccccag agcagtattg aactgccgaa tagacatccc acaatggcca atatccgcag
     1801 acgcatgatt cctcctgagt aagctggggc ttgaaggtgc actcgatctc acgtgattgc
     1861 aagagggccc cacagccgaa gccaatcttc tgattgatat aatcgtcaat attccataac
     1921 cgactgcaca aagcgatgcc ggattgtaat tgcagtttgt ggagactccc aaacaattac
     1981 agggcgctgc cattttacga ctattccagg cgggctacaa gggctaaaaa tcatgagtgg
     2041 gagggcagct gcgtctatat ccatgaatgg atggttgcct tcggggactc ttcagtttgg
     2101 tgtcgctcga taaccattca ctgagaccag gggtttttga cgggattgtg caagatgctg
     2161 atggttttcc agcagttgcc tctggttaat cccgtgccgg atcaaacgat aattttaggt
     2221 tttggtccat tatggattgc cacgttgagg actgcgctca tggaaaaaac acctcaccgc
     2281 aacccgggtg taaattctgc gtataccta
//
LOCUS       DAOPVS010000245         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_50337_length_2277_cov_44.693969, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000245 DAOPVS010000000
VERSION     DAOPVS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50337_length_2277_cov_44.693969"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKL11_11215"
     CDS             <1..311
                     /locus_tag="PKL11_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14213.1"
                     /translation="RVIVEEDSYGIPPGQPVPADVRLAAFMALPLETQRQVIMLIDYF
                     GNILNQGIGEMDMPFGFRPVGEWQALFAAQGFTVTQTIVQGFQRGLFNQTCHVWFVLD
                     "
     gene            372..1907
                     /locus_tag="PKL11_11220"
     CDS             372..1907
                     /locus_tag="PKL11_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNZ14214.1"
                     /translation="MTARRYPPRKFGARREQRQVEDLLSRFVRASPTAISVATRADGR
                     YLNVNDGFLQLFGYSRAEVIGHSATELNLWVDPADRSRVLAAFQSQKNLANLEARFRT
                     RTGEIITGLISVEDIILHGQECILVMLTDITGRKQAEDLLLASYNQLAEASDLARSLS
                     EILTPEEVYERLEQVTRRILPDISYVYISRYHPADQIITCVYAANEQQRLDLAQFSPI
                     PLAPPGQGTQSQVIHTRQPLVVNDFSERIPQMRVYHQTDPQRQTAASAYVPMVAHGQV
                     LGLFQVQSFTPGRFSQSDTLILSLLGNTAAIALDNARLFGDLLEAYDGIIEAWGRVLE
                     LRDQDTEGHTRRVTDLTVQVGRRMGLDETALANLRRGALLHDIGKLAIPDRVLLKPGP
                     LDADEWDLMRKHPLYAYQMLQPLAFLQPVTDIPLCHHERWDGEGYPHGLIGTDIPLPA
                     RIFAVVDVWDALNSARPYRPAWPQPEIITYLQAHAGTRFDPDVVRVFLELLETETGLP
                     PQP"
     gene            complement(1904..>2277)
                     /locus_tag="PKL11_11225"
     CDS             complement(1904..>2277)
                     /locus_tag="PKL11_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14215.1"
                     /translation="GAVILPLRSEQIGAHRARQQARINRGMMLGMILIILLTVVFMRP
                     IAQFFASLGNLGMAAPLFIALAVSFLALGLMAFVSALLAKLTPGPALQSAAELHQQAA
                     AALQPVQVELPDFEKDETAAG"
BASE COUNT          431 a          745 c          675 g          426 t
ORIGIN      
        1 gccgcgtgat tgtggaggaa gacagctacg gcattccgcc gggccagcct gtgccggccg
       61 acgtgcggct ggccgccttt atggcgctgc cgctcgagac ccagcgccag gtgatcatgt
      121 tgatagacta tttcggcaac atcctcaacc agggcattgg cgagatggat atgccctttg
      181 gcttccggcc ggtgggggag tggcaggcgc tgtttgccgc ccagggcttc accgtcaccc
      241 agaccatcgt ccagggcttt cagcgcgggc tgttcaatca gacctgccat gtgtggtttg
      301 tgttggattg aaatcttccc ccggcagttg atttgatttt ttagcgatta aacgctatga
      361 tttaacgtat tatgaccgcc cgccgatacc ccccgcgaaa atttggcgcc cgccgggaac
      421 agcgccaggt tgaagacctg ctgtctcggt ttgtgcgcgc cagccccact gccatcagcg
      481 tggcgacccg tgccgatggg cgctatttga atgtcaatga cggctttttg caattattcg
      541 gctacagccg ggccgaggtg attggtcaca gcgcaaccga attgaacctg tgggttgatc
      601 ccgcagaccg cagccgggtc ttggctgctt ttcagagcca gaaaaacctt gccaatctgg
      661 aggcgcgctt ccgcacccgc accggcgaaa tcatcaccgg gctgatctcg gtggaagaca
      721 tcatcctgca cggacaggaa tgtattctgg tcatgctgac ggatatcacc gggcgcaaac
      781 aggccgaaga cctgctgctg gccagctaca atcaactggc cgaagccagc gacctggccc
      841 gctcgctgtc tgaaattctg acccccgaag aagtctatga acggctggag caggtaaccc
      901 gccggatact gcccgacatc agctatgtgt acatttcccg ctaccacccc gccgaccaga
      961 tcattacctg cgtctatgcg gccaacgagc agcagcgcct ggacctggca cagttttcgc
     1021 ccatcccgct ggcgccgccc ggtcagggca cccagagtca ggtcatccac acccgccagc
     1081 ccctggtggt gaacgatttc agcgagcgca tcccgcaaat gcgcgtctat caccagaccg
     1141 acccgcagcg ccagaccgcc gcctcagcct atgtgcccat ggtggcgcac gggcaggtgc
     1201 tggggttgtt ccaggtgcag agctttaccc cggggcgctt cagccaaagc gataccctga
     1261 tcctcagcct gctgggcaat accgcggcca tcgccctgga taatgcccgc ctgtttggcg
     1321 acctgctgga agcctacgac ggcatcattg aagcctgggg gcgggtgctg gaactgcgcg
     1381 atcaggatac cgaaggccac acacgccgcg tcaccgacct caccgtgcag gtggggcgcc
     1441 gcatggggct ggatgaaacc gccctggcca acctgcgccg cggcgcactg ctgcacgata
     1501 tcggcaagct ggccatcccc gaccgggtgc tgctcaagcc ggggccgctg gacgccgatg
     1561 agtgggattt gatgcgcaag cacccgctgt atgcctatca gatgctgcag ccgttggcct
     1621 ttttgcagcc cgtgaccgat attcccctct gtcatcacga gcgctgggat ggcgagggct
     1681 acccgcacgg cctgatcggc accgacattc cactgccggc ccgcattttc gccgtggtgg
     1741 atgtgtggga cgcgctcaac tctgcccgtc cctaccgccc ggcctggcct cagcccgaaa
     1801 tcatcaccta cctgcaggcg cacgccggca cccgttttga tccggatgtg gtgcgcgtct
     1861 tcctggaact gctggaaacg gaaaccgggc tgcccccgca gccctaaccc gcggcggttt
     1921 cgtctttctc aaaatcaggc agttcaacct gaaccggctg caaggccgct gccgcctgct
     1981 ggtgcagctc ggcggcgctc tgcagcgccg ggccgggcgt cagcttggct aacagcgcac
     2041 tgacaaaagc catcagcccc agcgccagaa agctcacagc cagggcaatg aacaggggcg
     2101 ccgccatacc caggttgccc aggctggcga aaaactgcgc aatggggcgc atgaagacca
     2161 ccgtcaatag aatgatgaga atcatgccca gcatcatccc gcggttgatc cgcgcctgct
     2221 gacgggcgcg gtgtgcgcca atttgctcag agcgcagcgg taaaatcacg gccccgg
//
LOCUS       DAOPVS010000246         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_50579_length_2270_cov_60.967043, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000246 DAOPVS010000000
VERSION     DAOPVS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50579_length_2270_cov_60.967043"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKL11_11230"
     CDS             <1..638
                     /locus_tag="PKL11_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14216.1"
                     /translation="HFKGWTLGGLVFQALLFGGAGLLVLLLNPAPAALLIPAGLAAYP
                     FVRVRSKANTVRKQAVRALPEAAGLIAAELAASVPVETALVRAAALPGPLAILIQESV
                     ENARATGRPLFSRKPVRGAMVEVFAETRLPQLIAFATQLDLVAVKGVGGAELMSEIAR
                     SLGQEYRGRLVMEVEKLDSRLMVMVSLFFFIPFVALLLGALMIPTLQMMGG"
     gene            909..1817
                     /locus_tag="PKL11_11235"
     CDS             909..1817
                     /locus_tag="PKL11_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNZ14217.1"
                     /translation="MILRIRYLVLVALLALGIYAGGQLLPLASGDPVSLTAAPTPSQA
                     LTTQTAVPTPTRAAATPQPTPTRQQPTPAPDLAPTCARIDALDEAERTLALSQLTWAP
                     DYAGAVVLPSCNLAGENTPRAVVLHYTRGSLVGAVSRFQSPNEASAHYVVDRDGRVYQ
                     MIPERYGAYHASCRGERSYCQAACPICEDEQGHFLEPYSQSIGIEIVNWGPVGDPAAF
                     TEYPLYEDYNQAYGYRYWEDYSPEQVAAVKALVEDICARWNIPLDPAHVFGHARVNKK
                     ADPGPALNLFWDRYGEPPREAIWPQP"
     gene            1802..2242
                     /locus_tag="PKL11_11240"
     CDS             1802..2242
                     /locus_tag="PKL11_11240"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNZ14218.1"
                     /translation="MAAALIFGLTAVWLSVCAWSDWRTRQVSNWLTLPAVPVFLALRL
                     AEVGEPAPVTVTVMAVGAFALFWYFNAMGGADVKALIAMSLYHPGLALAALLGSLLWA
                     FILKRLHKSDLSRRWAGFPGFALGMGVIETGVLINLILKTWSKP"
BASE COUNT          351 a          676 c          759 g          484 t
ORIGIN      
        1 ggcatttcaa aggctggacc ctgggcggtc tggtgtttca ggccctgctg ttcggcggtg
       61 caggtctgct ggtgctgctg ctcaaccctg cgcctgctgc gcttctgatt ccggcagggc
      121 tggcggcgta tccgtttgta cgggtgcggt cgaaagccaa taccgtccgc aaacaggcgg
      181 tgcgcgctct gccggaagcc gccgggctga ttgccgctga actggcggcc agtgtgccgg
      241 tggaaacagc cctggtgcgg gcagccgccc tgccgggacc gctggcgatc ctgattcagg
      301 agtcggtgga gaatgcccgc gccaccggac gcccgctgtt ttctcgcaag ccggtgcgcg
      361 gcgccatggt ggaggtgttc gccgagacac gtttgcccca gctgattgcc tttgccaccc
      421 agcttgatct ggtggcggtg aaaggcgttg gcggcgccga gttgatgagc gagatcgccc
      481 gctcgctggg gcaggaatac cgcggccggt tggtgatgga ggtggaaaag ctggattccc
      541 gcctgatggt gatggtttcg ctgttcttct tcattccctt tgtggcgctg ctcctggggg
      601 ccttgatgat ccccaccctg cagatgatgg gcggctgacg tggcgctcga ctggtatttc
      661 ttgctgggcg ctgcggccgg agctgccgcc ggactgctga tccgcaaggc catttaccat
      721 gcggtgcgca gccaggagga tgacgaccca tgacgctggc tgtgttctct ctggtggtgg
      781 gctttgtgtt tggcatcagc tgcggttgga tcctgggcca ggcgctcgat tttatgggtt
      841 cttaggaggt gacggttgat tgacctgatt tcaggttttc ggacattgat cccgggcagg
      901 cgtgtgacat gatcctgcgg attcgttatc tggttctggt tgcgctttta gcgctgggca
      961 tctacgccgg tgggcagttg ctgccgctgg cctctggaga cccggtcagc ctgacagcgg
     1021 cgcccacgcc cagccaggct ttgaccacgc aaacagccgt tccgacgccc acccgggcag
     1081 cggccacacc gcagcccacg ccgaccaggc agcagccaac cccggcgcct gatctggcgc
     1141 cgacctgtgc ccggattgat gccctggacg aggctgagcg caccctggcg ctgtctcaac
     1201 tgacctgggc gccggattat gccggagccg tggtgctgcc atcctgcaat ctggcaggcg
     1261 agaatacacc ccgcgccgtg gtgctgcact acacccgcgg cagtctggtt ggcgccgtga
     1321 gccgcttcca atcccccaac gaggcctcgg cgcattatgt ggtggatcgg gatggaagag
     1381 tttatcagat gatccccgag cgctacggcg cttaccatgc atcctgccgg ggggagcgga
     1441 gctactgcca ggcagcctgc ccgatctgcg aggatgagca gggtcacttt ttggagccgt
     1501 acagccagag catcgggatc gagatcgtca actgggggcc ggtgggcgac ccggcagcct
     1561 ttaccgaata cccactgtat gaagattaca accaggctta cggctaccgc tactgggagg
     1621 attactcgcc ggagcaggtg gcggctgtga aagcgctggt agaggatatc tgcgcccggt
     1681 ggaacattcc gctggacccg gcgcatgtgt ttggacatgc gcgggtcaat aagaaggccg
     1741 accccggccc ggcgctcaat ctgttctggg atcgctacgg cgagccgccc cgcgaggcga
     1801 tatggccgca gccctgatct ttggcctgac tgctgtatgg ctgagcgttt gcgcctggtc
     1861 agactggcgc acccggcagg tctccaactg gctgacgctg cccgccgtgc cggtctttct
     1921 ggcgctgcgc cttgctgaag tgggcgagcc agccccggtc acggtgacgg tgatggcggt
     1981 gggcgccttt gcgctgttct ggtacttcaa tgccatgggc ggggccgatg tcaaagcgct
     2041 gatagccatg tcgctgtacc atcctggcct ggcgctggct gctctgctgg gcagcctgct
     2101 gtgggccttc attctcaaac gcctgcacaa atccgatctc tcccgccgct gggccgggtt
     2161 ccccggcttt gccctgggaa tgggtgtgat agaaaccggt gtgttgatca atcttattct
     2221 aaaaacctgg agtaaaccat gagcaaatat gtcattgaca tgggcatgct
//
LOCUS       DAOPVS010000247         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_50580_length_2270_cov_58.626185, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000247 DAOPVS010000000
VERSION     DAOPVS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50580_length_2270_cov_58.626185"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKL11_11245"
     CDS             <1..684
                     /locus_tag="PKL11_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ14219.1"
                     /translation="LTAGIISGLVLFFGASLQQVGIVETTAGNAGFITGLYVVIVPVL
                     GLFLRQRTGWATWLGAALAAVGLYLLSVTAQFTMGRGDAFVLIGAFFWAIHVLVIGYF
                     SRRVDPLWLSIIQFLTCAILSGVTAFTTESIHPALIWTARWPILYGGLLSVGVAYTLQ
                     VISQQKAHPAHAAILLSLESVFAALGGWLIMGEQLSARGLLGCGLMLAGMLISQLAAF
                     RPSAARTPA"
     gene            718..1752
                     /locus_tag="PKL11_11250"
     CDS             718..1752
                     /locus_tag="PKL11_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14220.1"
                     /translation="MKKPGFYLISLALLLTLLAGCNLPTGLPGVYTPAALQTPVPGTP
                     LYLPLIERQTSETPTSTVAVQVELPTPTTPPPTPTPEGLYSGPITDPERTGQTLSPDE
                     WWDWPVLPVVSDRARQIYFWGLAHGTDPNRFSKIGDCQVIRQYFLGVFDDPTLYRLGP
                     HEDLIPTIDHFHGSWDRVSMSVRTGFNVASVLTAINADAAQCLPGEIPLECEFRIHNP
                     SIAIISMETWTADRPVELYEQYLRQIVEFTIGRGVVPILATKADNLEGNHAINAAIVR
                     VAYDYDIPLWNWWRVADALPDHGLTSDGFHLTHASNFFDDESAMEAAWPNRNLSGLQA
                     IDVVYRELNR"
BASE COUNT          434 a          688 c          565 g          583 t
ORIGIN      
        1 ctcaccgcgg gcataatctc aggcctggtg ctctttttcg gggcctcgct tcagcaggtc
       61 ggcattgttg aaaccactgc tggtaacgct ggctttatca ccggtctgta tgtggtcatt
      121 gtgccggttc tcggcctctt cctgcgccag cgaaccggct gggccacctg gctgggtgct
      181 gccctggccg cagttggcct ctaccttttg agcgttacgg cgcaattcac catgggccgc
      241 ggcgatgctt ttgttttgat tggcgccttt ttctgggcca tccatgtcct ggtaatcggt
      301 tacttttccc gccgggtgga tccgctctgg ctctccatca tccaatttct tacctgcgct
      361 atcctcagcg gcgtgactgc cttcaccact gagtcaattc atcccgccct catttggacc
      421 gcccgctggc cgattttgta tggcggttta ttgtccgtcg gtgtggccta caccttacag
      481 gtgatctcac agcaaaaagc ccatccggcg cacgcggcca tcctgctcag cctggaatcg
      541 gtctttgccg ccctgggcgg ctggctgatc atgggcgaac agctctcagc gcgtggtctc
      601 ctgggctgcg gtctgatgct ggctggaatg ttgatctctc agctagctgc ctttcgcccg
      661 tctgctgcca gaacccctgc ttaacctcat aggatataat tactaccagg aggccgtatg
      721 aaaaaacctg gtttttatct gatatcgctt gccctgttac tcaccctgct tgctggctgt
      781 aatctgccca ccgggctgcc cggagtctac accccggcgg ctctccagac gcctgtgccc
      841 ggaacccctc tataccttcc tctgattgag cgtcagactt cagaaacgcc cacctcaacg
      901 gttgccgttc aggttgaact tcctaccccc acaacgccgc cccccacacc gacgccagag
      961 ggcttatact ctggccccat cacagacccg gaacgcactg gccaaaccct ctcgccagat
     1021 gaatggtggg attggcccgt tctgccagtc gtcagtgacc gggcgcgcca gatttacttt
     1081 tggggtctgg ctcacggcac tgatccgaac cgcttttcaa aaattggcga ttgtcaggtc
     1141 atccgccaat atttcttggg cgtcttcgac gacccaactt tatatcgcct tggcccacat
     1201 gaagatttaa tccccaccat tgaccatttc catggtagtt gggatcgggt ttctatgtct
     1261 gttcgaacgg gtttcaatgt ggcttcggtc ttgaccgcaa tcaacgccga tgccgcccag
     1321 tgtctgcccg gcgaaattcc tctggaatgc gagtttcgca ttcataaccc ctccattgcc
     1381 attatcagca tggaaacctg gactgccgat cgtcctgttg aactttacga acaatacctg
     1441 cgccagattg tagaatttac catcgggcgc ggggttgtcc ccattcttgc caccaaagcc
     1501 gacaatcttg aaggcaacca tgccatcaat gccgccattg tacgcgtggc atatgattat
     1561 gatatccctc tctggaactg gtggcgcgta gccgatgccc tgcccgacca cggcttaacg
     1621 tcagatggat tccatctgac gcacgcttcc aacttttttg atgatgaatc tgcaatggaa
     1681 gcagcctggc caaaccgcaa tcttagcggt ttgcaggcga tagatgtcgt ttatcgggaa
     1741 ctgaatcgct gattaacgaa tgggataaat aagattaata gcaactcgca tagactttgg
     1801 acgctgctct actcgcacaa ccttcccggc caggtttaat ggctcgggtc ggtctgcccg
     1861 cgtaaattgc agcttgattt cgtccccgat gtggacatca aattctgggg taagccggag
     1921 cagtagcccg caggcgctga cgttgactgt tgtggcggat ccactcattg gctgaaccag
     1981 ctttacgggc tgcttgatcg cgaatcggtt agcccgtctt cgttcaggag agcctatttg
     2041 ggtcgaagca cacaccgcag aatcctcctt cgcgccgagc gcaatatcat atgttactat
     2101 agcacaaaat gcccccctct gcgcactgag ttttaatccg ttactcataa acccccgcac
     2161 ttctgatcaa gtgcgggggt tcaatgacaa tttgaaagcc tggtgttcta tttgcttgcc
     2221 agccgggcgc gcacatcaat ggcaaatgcc ccgcccttgc ctggcagcgc
//
LOCUS       DAOPVS010000248         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_50581_length_2270_cov_46.980587, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000248 DAOPVS010000000
VERSION     DAOPVS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50581_length_2270_cov_46.980587"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..707)
                     /locus_tag="PKL11_11255"
     CDS             complement(69..707)
                     /locus_tag="PKL11_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14221.1"
                     /translation="MSILNQLSSQVGDRTEYANRRVAALCLQDPNLIDAIAAGLEGSN
                     AALMGDCAEVLTLVAEQHPEWAAPHAEALAARLSHKNTRVRWEAMHALALIAMRTPEV
                     IAPRLSTLGELIQQDASVIVRDYAVDAVGHYAAAGPQAAEAAYPWLVSALSVWNGKHA
                     GHALTGLIHAARHLPDRHTQLAAIADDYARSERAVIRKAAKGLQKALKGLPG"
     gene            821..1630
                     /gene="surE"
                     /locus_tag="PKL11_11260"
     CDS             821..1630
                     /gene="surE"
                     /locus_tag="PKL11_11260"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNZ14222.1"
                     /translation="MKRKQILLTNDDGILSPGLWAAAEALGALGFVTVVAPRRQYSSA
                     GRSLPNDSDGRIERQHLQIGTQTWEVYAVGGSPAQAVQHALLEVMPQPPDLVVSGINY
                     GENVGTGVTISGTVGAALEAAAMGIPGLAVSMQLAVVEDFLSYSRSVDFSAAAHFTAQ
                     IAERMLAAPLPPDVDVLKVEVPLGATPQTPWRITRQSRHRYYDPYPEREGDWSQPGHI
                     RARVNVQPGEVTPDSDVHTLYFDRLVSVTPLSLEMTSRVDLGTLDQQLRRP"
     gene            1627..>2270
                     /locus_tag="PKL11_11265"
     CDS             1627..>2270
                     /locus_tag="PKL11_11265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ14223.1"
                     /translation="MTLPPPAETFRRLCCNPAVLDFASATLTEAYCTAAPDHEQPMRA
                     LMDAAFALLRTTAADPARFATALHTLSNDWFAPGGSQPWFKAVYLDYKQRIKPPVRYA
                     RLDPHLRGQAVLDFGCGNGLTALILAQNGWQVQLADVLDYRSPAARGLPFTPLGAETH
                     LPYPDNAFDTALVFAVLHHVQPAALLPLVGELRRVSRRVIVEEDSYGIPPGQPVP"
BASE COUNT          382 a          791 c          683 g          414 t
ORIGIN      
        1 cgggccatct accagcgcgg tgaggcaaaa atcaagcact tcaccaccga aaaggtctag
       61 gggctgcctc accccggcag gcccttgagc gccttctgca agcctttggc cgccttgcga
      121 atcacagccc gctcgctgcg ggcgtaatcg tcggctatcg ccgcaagctg cgtgtgccgg
      181 tctggcaggt gcctggctgc atggatcagc ccggtcagcg cgtgcccggc atgtttgcca
      241 ttccagaccg aaagcgcgct caccagccag ggataggccg cctcggcagc ctgcggcccg
      301 gcggcggcgt aatggcccac cgcatcaacc gcataatcgc gcacaatcac gctcgcatcc
      361 tgctggatga gttcccccag ggtggacagc cgcggtgcaa tcacctccgg cgtccgcatg
      421 gcaatcagtg ccagcgcgtg catggcttcc cagcgcaccc gcgtgttttt atggctcaac
      481 cgcgcggcca gggcttcggc atggggcgca gcccattcgg gatgctgctc agccaccagc
      541 gtcagcacct cggcgcagtc acccatcagg gctgcattgg agccttcaag cccggcagca
      601 atcgcatcaa tcaaattggg gtcttgcagg cacagcgccg ccacccggcg gttggcatac
      661 tcggtgcggt cacccacctg cgaagataac tggttgagaa tggacatggc tcgcctcctg
      721 ctgcttgcac acaactgtta gttgcaataa aacggctctg gaatgattat atcccgcccg
      781 acaccgtttc taaggtaaaa ttatcgccag aaagtgaccc atgaaacgca aacaaatctt
      841 actcaccaat gatgatggta ttctctcgcc cgggctctgg gctgccgccg aggcgctggg
      901 cgccctgggc ttcgtcactg tggtggcgcc gcgccgccag tattccagcg cggggcgctc
      961 gctgcccaac gactctgatg gccggattga gcgccagcac ctgcaaattg gcacccagac
     1021 ctgggaggtc tatgccgtgg gcggctcgcc ggcccaggcc gtgcagcatg ccctgctgga
     1081 agtgatgccc cagccccccg acctggtggt ttcgggcatc aattatggcg aaaacgttgg
     1141 caccggcgtg accatttcgg gcacggtggg tgccgcgctg gaagccgccg ccatgggcat
     1201 ccccgggttg gcggtttcga tgcagcttgc cgtggtagaa gactttttaa gctactcccg
     1261 ctcggtggat ttttcggccg cggcgcactt taccgcacag atcgctgagc gcatgttggc
     1321 cgcgcccctg ccccccgatg tggacgtgtt aaaggtagag gtgccgctcg gcgccacccc
     1381 ccagaccccc tggcgtatca cccgccagtc acgccaccgc tattacgacc cctaccccga
     1441 gcgcgagggc gactggagcc agccggggca tattcgggcg cgcgtcaatg tgcagcccgg
     1501 tgaggtgacc cccgacagtg atgtgcatac cctttacttt gaccggctgg tctcggtgac
     1561 cccgctgagc ctggagatga cctcgcgcgt agacctgggc acgctcgatc aacagctgcg
     1621 ccgcccatga ccctgccgcc ccctgccgag accttccgcc gcctgtgctg caaccccgcc
     1681 gtgctggact ttgccagcgc cacactgaca gaggcctact gcaccgccgc gccggatcac
     1741 gagcagccca tgcgtgcgct gatggacgcg gcctttgcac tgctgcgcac caccgccgcc
     1801 gaccccgccc gctttgccac cgcgctgcat accctgtcaa acgactggtt tgctccgggc
     1861 ggctcacagc cctggttcaa ggcggtttat ctggattaca aacagcgcat caagccgccg
     1921 gtccgctatg cccggcttga cccgcacctg cgcggccagg cggtgctcga ttttggctgt
     1981 ggcaacggcc tgacggcgct gattctggca cagaacggct ggcaggtgca gcttgccgac
     2041 gtgctggatt accgcagccc tgccgcgcgc ggcctgccct tcacgccgct gggcgccgag
     2101 acgcacctgc cctaccccga taacgccttt gataccgccc tggtatttgc cgtactgcac
     2161 catgttcagc ccgcagcctt gctgccgctg gtgggcgaac tgcgccgggt gagccgccgc
     2221 gtgattgtgg aggaagacag ctacggcatt ccgccgggcc agcctgtgcc
//
LOCUS       DAOPVS010000249         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_50975_length_2259_cov_60.556261, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000249 DAOPVS010000000
VERSION     DAOPVS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50975_length_2259_cov_60.556261"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="PKL11_11270"
     CDS             <1..1551
                     /locus_tag="PKL11_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNZ14224.1"
                     /translation="QRQVNIRTAELARSEEKYRLLVENASEAITVICDGKLVFANSRI
                     YNLTGYPPEQLAHHSLWNIIHPDDRAIVIDRYERRISGEDILSQYPIRIITADGATRY
                     VLIQSVRIEWEGRLAALSLYTDITDRVLAEKELERQLSRLDALRAVDMAILSSEALPI
                     VLSALLENLSSQLQVDAADILMLNETESTLEWVAGVGFNEYLTEEVALRYPLAPYFAG
                     QVAMQKIPRLLVNLTATDPQMLLPPARSAGFVLYCALPLVAKGEVKGVMEIYHRKQIE
                     MEADCLGFLRTMSDQAAIAIDNAILFASLQRTNQDLLEAYDATIRGWARALDLRNSEA
                     GDHTRRLVNFTLNLASRLGIPIEQWEHIRRGVILHDIGKMAVPDEILLKPGPLTPEEW
                     EVMRRHPQYAHDWMSHIPFLQPAMDIPYGHHERWDGSGYPLGLKGEEIPLAARIFAVV
                     DVWDALTHDRVYHSAWAHNEALTYLETNSGKLFDPQVIDVFIQYLEEETQAQSDGAAK
                     QPHQEAED"
     gene            complement(1554..2240)
                     /locus_tag="PKL11_11275"
     CDS             complement(1554..2240)
                     /locus_tag="PKL11_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14225.1"
                     /translation="MKALKSILLVVIVLSMAALACDLPELPFGNGTEEPNTNPIPVDG
                     GNDVLFKDDFSKTGSGWDRYDDDGSVTDYADGGYRILVGNENWYNWANPYKSFTDVRV
                     EVDVTKQASPDGDAGIICRYVDTSNFYFLRITTDGYYAISKLVDGTETTLGAGEYTLT
                     DAVNTGVGEVNHVRADCVGSTLTLYVNGEKLEQVTDSTFDSGDVGLLGGSFETGGVDL
                     LFDNFIVYQP"
BASE COUNT          498 a          678 c          574 g          509 t
ORIGIN      
        1 caacgccagg tgaatattcg caccgccgaa ctggcccgca gcgaggaaaa ataccgcctg
       61 ctggtagaaa acgccagcga ggccatcacc gtcatctgtg atggcaaatt ggtttttgcc
      121 aattcgcgca tttacaacct taccggctat ccgcctgaac aactggcgca tcacagcctg
      181 tggaatatca ttcatcccga tgaccgggcg atcgtcatag atcgttatga gcgccgcata
      241 tccggcgaag atattctgtc gcagtatccc atccgcatca taaccgccga tggcgccacg
      301 cggtacgtgc tgatccaatc agtgcgcatc gaatgggagg gccgcctggc agccctcagc
      361 ctttacaccg atattaccga ccgtgtcctg gctgaaaaag aactggaacg gcagctctcc
      421 cgcctggatg ccctgcgcgc agttgatatg gctattctga gcagcgaagc gcttcccatt
      481 gttttaagcg ccctgctgga aaacctctcc agtcaattgc aggtggatgc cgctgatatt
      541 ctgatgctta acgaaacgga atcaaccctg gaatgggttg ccggcgttgg cttcaacgaa
      601 tatctgaccg aggaggtagc cctgcgttat ccgcttgcac cttattttgc gggtcaggta
      661 gccatgcaga agatcccccg cctgctggtc aatcttactg ccacagaccc ccaaatgctg
      721 ctgccccctg cccgctcggc tggctttgtc ctttactgcg ccctgcccct ggttgccaaa
      781 ggcgaagtca agggcgtgat ggaaatttac caccgtaagc agatagagat ggaggcagat
      841 tgccttggct tcttgcggac catgtcagat caggctgcca ttgccattga taatgccatc
      901 ttatttgcca gcttgcagcg caccaatcag gacttactcg aagcttacga tgccaccatt
      961 cgcggctggg cgcgggcgct tgacctgcgt aactctgaag ccggagatca tacccggcgg
     1021 ctggtcaact ttacccttaa cctcgccagc cggctgggaa tccccatcga gcagtgggag
     1081 catattcgcc gcggcgtcat cctgcacgat attggcaaaa tggccgttcc agatgagatt
     1141 ctgctcaaac cgggcccact gacccccgaa gaatgggagg tcatgcgccg ccatccccag
     1201 tacgcccatg attggatgtc gcacatcccc ttccttcagc ccgccatgga tattccctat
     1261 gggcaccacg agcgctggga tggctctggc tatccgctgg gcctcaaagg cgaagaaatc
     1321 ccgctggctg cccgcatctt tgctgtggtt gatgtctggg atgccctcac ccacgaccgg
     1381 gtctatcact ccgcctgggc gcataatgag gccttaactt atcttgagac taattccggc
     1441 aaattatttg acccccaggt gattgatgtg tttatccagt atcttgagga agaaacccaa
     1501 gctcagtctg atggggctgc aaagcagccc catcaggaag cagaagactg agtttacggc
     1561 tgatagacaa taaaattgtc aaacagcagg tcaacgccgc cggtctcaaa cgagccgccc
     1621 agcaggccca catcgcccga atcaaacgtg ctgtcagtta cctgctccag cttctcgcca
     1681 ttcacataca gcgtcagcgt gctgcctacg cagtctgccc gcacgtgatt gacctctcca
     1741 acgcccgtat tgaccgcatc ggttaacgta tattctccgg cgcccagggt tgtttcggta
     1801 ccatcaacca gtttcgagat cgcgtaatag ccatcggtgg taatccgcag gaagtagaag
     1861 ttggaggtat ctacataccg gcagataatg cccgcgtcgc catcaggact ggcctgcttg
     1921 gttacatcca cttccacccg cacatcggtg aagcttttgt acggattggc ccagttgtac
     1981 cagttttcgt tcccaaccag aatccggtag ccgccatcgg cataatcggt cactgagccg
     2041 tcgtcgtcat accgatccca gccgctgccg gtctttgaga aatcgtcctt gaaaagcaca
     2101 tcattcccgc cgtcaacagg gatggggttt gtgttgggct cttcggtgcc attgccaaaa
     2161 ggcagttccg gcaggtcgca ggccagggcg gccatgctga gcacaatcac aaccagcaaa
     2221 atcgacttta acgctttcat tattacactc cttgtgtca
//
LOCUS       DAOPVS010000250         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_51074_length_2256_cov_59.144025, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000250 DAOPVS010000000
VERSION     DAOPVS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51074_length_2256_cov_59.144025"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..509
                     /locus_tag="PKL11_11280"
     CDS             111..509
                     /locus_tag="PKL11_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ14226.1"
                     /translation="MELQLDLDLRSDVPVYLQIVEQVQRQVLSGQLRPNDQLPTVRQL
                     AAQWRINFNTVARAYRLLDEAGLISTQHGRGTFILEPADPAGQEHLRQAALASLIARA
                     WDEAARLGYTPTEITAAFNEEHQRRLRADH"
     gene            536..1483
                     /locus_tag="PKL11_11285"
     CDS             536..1483
                     /locus_tag="PKL11_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNZ14227.1"
                     /translation="MQPKTSTNVTFTRKTEPGFNALSGALMTVCLLIGIIGAVLMDGR
                     VPDGWIAVFAIFFILLGLFFFYALRVARQWEKVIVLRLGRFLGLRGPGVFWLIPLLDT
                     AAAWIDQRVMVTPFNAEKTLTRDTVPVNVDAVLFWVVWDAEKAALEVEDYRSAIAWAA
                     QTALREIIGQMTLADILVGRDKMDAELQAIIDQRTAPWGITVQSVEIRDVIIPSDLEA
                     AMSRQAQAERERQARVILGESEMQIAESFASASQAYINNPTALHLRAMNMLFEGLKEK
                     GAMVIVPSSAVDTMNLGATSGLISLAQNADRQAKEDEQS"
     gene            1548..1754
                     /locus_tag="PKL11_11290"
     CDS             1548..1754
                     /locus_tag="PKL11_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HNZ14228.1"
                     /translation="MDFDIQRYLPLLIPIALLQLGLMIYALVDLIRRQRTKGPKWMWA
                     LIILLINFIGPVVYLLAGREEDEA"
     gene            1751..>2256
                     /locus_tag="PKL11_11295"
     CDS             1751..>2256
                     /locus_tag="PKL11_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ14229.1"
                     /translation="MSAIQAQGLRKAFGPFVALDGLSLTVEPGAVFGFLGPNGAGKTT
                     TIRILAGLARADAGQVSVAGVDLRADGHLLSRRVGYLPEEPAFYPWMSAVEFLDYVGR
                     LYGQTPAERKTRTAELLELTGLTEARKRRVGGYSRGMRQRLGLAQALYHRPEVLLLDE
                     PVSALDPAG"
BASE COUNT          418 a          712 c          701 g          425 t
ORIGIN      
        1 gcaccgccgc acctgaaacc tgaaacttga aacctgaaac atcttgacat ttctgtaaaa
       61 cgatgtacaa tgatacaaag atacaatcat acaatcaagg agattggctg gtggaactgc
      121 aattggattt ggatctgcgc tcagatgtgc cggtgtatct gcaaattgtg gagcaggtgc
      181 agcggcaggt gctcagcggt cagctgcgcc ccaacgacca actgcccacc gtgcgccaac
      241 tggcggcaca gtggcgcatc aacttcaaca cggtggcgcg ggcttaccgc ctgctggatg
      301 aggccgggct gatctccacc cagcacgggc gcggcacctt cattctcgaa cccgccgacc
      361 ccgccgggca ggagcatctg cgtcaggcgg ccctggccag cctgattgcc cgcgcctggg
      421 atgaggcggc ccgcctgggg tacaccccca ccgagatcac cgccgctttt aacgaggaac
      481 atcagcgccg actgcgcgct gaccactaac tttttttgtt tttttggagg atcgaatgca
      541 acctaagact tctaccaatg tgaccttcac ccgcaagacc gagcccggct tcaacgccct
      601 cagcggcgcg ctgatgacgg tgtgtctgct gatcggcatc atcggcgccg tgctgatgga
      661 cggccgtgtg cccgatggct ggattgcggt ctttgccatc ttcttcatcc tgctggggct
      721 gttcttcttc tacgcgctgc gggtggcccg ccagtgggaa aaggtgattg tgctgcggct
      781 ggggcgcttt ttggggctgc gcggcccggg cgtcttctgg ctgatcccgc tgctggatac
      841 tgccgccgcc tggattgatc aacgtgtgat ggtgaccccc ttcaacgcgg aaaagaccct
      901 cacccgcgat accgtgcccg tcaatgtgga cgccgtgctg ttctgggtgg tgtgggatgc
      961 cgaaaaggcc gccctggagg tggaagatta ccgcagcgcc attgcctggg ctgcccaaac
     1021 cgccctgcgc gagatcatcg gtcagatgac gctggccgac attctggtcg gccgcgataa
     1081 gatggatgcc gaactgcagg cgatcattga tcagcgcacc gccccctggg gcatcaccgt
     1141 gcagtcggtt gagatccggg atgtaatcat ccccagcgac ctcgaagccg ccatgagccg
     1201 ccaggcccag gccgagcgcg aacgccaggc ccgtgtgatt ttgggcgaaa gcgaaatgca
     1261 aattgccgaa agctttgcct cagcctcgca ggcctacatc aacaacccca ccgccctgca
     1321 cctgcgcgcc atgaacatgc tgttcgaggg cctcaaagaa aagggcgcca tggtgattgt
     1381 gccctcctcg gccgttgata cgatgaacct gggcgccacc agcggcctga tctcgctggc
     1441 gcagaacgcc gaccgccagg ccaaagaaga cgaacagtcg taaccgctaa ccaccgcccg
     1501 cgggctggtc aaaacaaccc gcgggcaatc gtttcaagga gtttgaaatg gatttcgaca
     1561 ttcaacgata cctgcccctg ctcatcccca ttgccctgct gcagctgggg ctgatgattt
     1621 atgccctggt ggacctgatc cggcggcagc gcaccaaagg ccccaaatgg atgtgggcgc
     1681 tgattatcct gctgatcaat ttcatcgggc cggtggtgta cctgctggcc gggcgagagg
     1741 aagacgaagc atgagcgcca ttcaagcgca gggactgcgc aaggccttcg gcccctttgt
     1801 ggcgctggac ggcctttctc tgacggtgga acccggcgcg gtgttcggct ttttgggccc
     1861 caacggcgcc ggcaagacca ccaccatccg cattctggcg gggctggcgc gggccgatgc
     1921 cggccaggtg agcgtggccg gggtggatct gcgcgccgat gggcatctgt tgagccgccg
     1981 ggtgggctac ctgcccgaag aacccgcctt ttacccctgg atgtcggcgg tggagtttct
     2041 ggattatgtg gggcggctct acgggcagac gcccgccgag cgcaaaaccc gcaccgccga
     2101 actgctggaa ctgaccggcc tgaccgaggc ccgcaagcgc cgcgtgggcg gctattcccg
     2161 cggcatgcgc cagcggctgg ggctggccca ggcactgtat caccgtcccg aggtgctgct
     2221 gctggatgaa cccgtcagcg cgctggaccc cgccgg
//
LOCUS       DAOPVS010000251         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_51075_length_2256_cov_23.493412, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000251 DAOPVS010000000
VERSION     DAOPVS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51075_length_2256_cov_23.493412"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..189
                     /locus_tag="PKL11_11300"
     tRNA            116..189
                     /locus_tag="PKL11_11300"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Asp,seq:gtc)
     gene            1446..1796
                     /locus_tag="PKL11_11305"
     CDS             1446..1796
                     /locus_tag="PKL11_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14230.1"
                     /translation="MRLVELIQRGQQPMQVAVEAVNLIHHNHLKAPGLRIGHQPFQCR
                     AVAVFLGRQPRIGVYLNHLPALLLGISLNIDGLRIQAVPIHLFTHRYPPVTGSALDIF
                     HTHPPFFGYNQVAQ"
     gene            1839..2252
                     /locus_tag="PKL11_11310"
     CDS             1839..2252
                     /locus_tag="PKL11_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ14231.1"
                     /translation="MALPIAKARRLTRQKDFDPMPLINRLRELLAATKDSYREASLKA
                     GLDHQAVRRILEGQRPAMHICILLADYFNINPNELLFLADWPPLRAFEIYEPRAEHLP
                     PEALEVALDIAKIPDADTRRQVADAIRILLKKYFA"
BASE COUNT          470 a          676 c          586 g          524 t
ORIGIN      
        1 ctggaagccc cctacgacac aacccgcagc ggctgggctg ctgcaaatta tttggcaatt
       61 gtctctgaaa atccttgacg ccaccccaaa ggcatgataa actattggcc tgttcggggg
      121 cgtggtgtag cggtcaaaca tgcggccctg tcaaggccga gatcgcgggt tcgaatcccg
      181 tcgctcccgc tgctacaaaa aacctctccc tctgggacga ggttttttgt tagttgaggc
      241 gcaaacgcag cagttggttc gatgcccacg attgcgttgc ctcttacgaa gagcgcttcg
      301 agtactagtc ggagcgcttt tcgcgtctca attgttaatg atggtgacaa tccattatct
      361 gaaccgtcaa acatctctaa tgctattcta ttagcattgt cgcaaaattg caaggggttt
      421 gtgtgaccaa tttcatcgaa tcttgctttc tcttgattat acacgtttgg ctccaactcg
      481 ccggccagat acagcgcctg tacgcgctga aaacgcgctt tagcctgttc gtaatgctca
      541 gctaatgctt tctgccgggc aatttcattg gggtcatgca gaatctttcc tagccactgg
      601 aagaccacct tctcgatgac tttagcattg accagccgct gcgggcagtc atgggtatgc
      661 tcaatttgac cggcatcccg atagtagcgg tagccattgg cagaaacccc gcgcatggtg
      721 ctgccgcagt acccgcagcg gatgatcccg ctgagcggga agatgcgcgc cgggtaattg
      781 ggcccggtac ggggtgtatt tgccatgact actcgaatgg cattcacttc tttgaagact
      841 tcataatcca ccagtgccgg gtgttgggca tcaaacagct caatatggtg tgaccgccgg
      901 cgtttgatgc cgttctcatt caccccgtag taggggattt tcccggcata cagcacccgc
      961 tgcagcatcg tacgaacagc ctcgcggccc agcgggccag gtgggcagtg ccccggccgg
     1021 cccttgctgc ggaagtgcac catttggccg ttccaaccag gcacttcagt gctgttgatc
     1081 aggtcagcaa tttgcgcatc tgaactttgc ccggtggcat attctgcaaa tgccagcttg
     1141 atgatctgac tctcgatcgg gtgggggata atcttgccct tttcggtgca gcagtccggt
     1201 tgaccgtagc gggggcagta atccgggcca ttgggatcgg tgcaggcgga gcagttgccc
     1261 cggcagtaac ccagcgggat gctgccgttc agcaggccct tgcgggcccg ttgcagcttc
     1321 cctttacggg tttcctgccc caggttttga atatagatct cggccagcat ccccagcacg
     1381 gtcagggtca gcttgcccca gggcgaggta aagtccagcc gttcctggac tgaggcaaat
     1441 cccacatgcg cctggttgag ctgatccagc gcggtcagca acccatgcag gtggcggtag
     1501 aagcggtcaa tcttatccac cacaatcacc tcaaagcgcc cggcctgcgc atcggccatc
     1561 aaccgttcca gtgccgggcg gtggctgtct ttcttggcag acaaccccgc atcggtgtat
     1621 atctcaacca cctcccagcc ttgctgttgg gcataagttt gaatattgac ggcctgcgca
     1681 tccaggctgt gcccatccac ctgttcacgc atcgataccc gccggtaacc ggcagcgcgt
     1741 tggatatttt tcatacccat cctccattct ttggatataa tcaagtagcg caatgattgc
     1801 gctcatggac taatcataca caagtttgag gaatttctat ggctctgcca attgcaaaag
     1861 cacgccgcct gacccgtcaa aaagactttg acccaatgcc gcttatcaac cggctgagag
     1921 aactgctggc cgcaaccaaa gacagctacc gcgaagcctc acttaaagcg gggcttgatc
     1981 atcaggcggt ccgccgcatc cttgaaggcc agcgcccggc catgcacatt tgcatcctcc
     2041 tggctgatta tttcaacatc aacccgaacg aactgctgtt tttggccgat tggccccccc
     2101 tgcgcgcctt tgaaatttac gaaccccgcg ccgaacacct gccccccgaa gcgctggaag
     2161 tggccctcga cattgccaaa atccccgacg ccgacacccg ccgccaggtc gccgacgcca
     2221 tccgcatcct gctgaaaaaa tatttcgcct gacctg
//
LOCUS       DAOPVS010000252         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_51364_length_2248_cov_23.932513, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000252 DAOPVS010000000
VERSION     DAOPVS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51364_length_2248_cov_23.932513"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..>2248
                     /locus_tag="PKL11_11315"
     CDS             208..>2248
                     /locus_tag="PKL11_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNZ14232.1"
                     /translation="MKKLLSFKPALVLSLLMAVFFNAITTPPLIYAQSPEPPTATEGE
                     TGQAQPEVSAVILGENTAGPQIDFGVIPLGGSGCTPAPGRWLLRASLPEAVYGNGVVS
                     DGTYIYSLGGFSSTAGLSDQFMRFDPQTNQWIDIPSAPTLVYAGLAVFAEGKIFLFGG
                     VDASAPISLVQIYTIATGTWSNGANMPAPRQQMGGGYYDGKIYLAGGFESDSVGSASN
                     QTWEYAPSSNSWAVKATMPAALAGPGSGIIGGALYLAGGRDSAGTSLNTAYRYDIASD
                     AWASIAALPNAVNYPGSAVYGQRLWIFGGGDPFLNDETGVENETESLSFTQIYNPHTN
                     SWSSGPSQNLARSFQGGAVVQNQIFSVGGYPGGMWSSNVVEAATQNPLKILMIYADNR
                     PPSVLQFLLMSQPGVGQVDVISANSSVIGAGTMMAYDVVVIFSNASFSNDVAQGDQIA
                     DYIDMGGAVVAFNFSFSSGYQLAGRWITDGYTPFNLSTGNAFSDVSLGSYVTGHPLMA
                     GVNSLNAYYHTNVSLAPGSGLVATWSDGTLAMAVNGQAVGVNAYVGEVPKNWSGDFAA
                     LIANAGYWLRDGNGFCIHQACTTPENITGALTDSDYTQTGRLARYDPASTCAAPTSCP
                     GPYNSTETHYDAYAFMNNTPATQCVTITLDPKGCVDTHYLQSAAYNGFLSNDLCAN"
BASE COUNT          493 a          572 c          641 g          542 t
ORIGIN      
        1 acgatgtcgc catggcgggt tcgatcacca tgacggggtt ggtggtgcag taagtttgta
       61 acgacaagcg tgctttgcca gccagatggg cctggaatga ttcggtgccc atctgtattt
      121 gcgcgaccat tcaccgactg gacgtataat cggcatatct tcgggcgcgc gttggcccat
      181 tccctctaac ctcaaggagg cattttaatg aaaaagttat tgtcgtttaa gcccgcgctt
      241 gtgctgagcc tgttgatggc tgtgttcttc aacgccatca caaccccacc gctgatttac
      301 gcccaatctc ctgaacctcc gacggcgacc gaaggtgaaa ctgggcaagc acagcccgag
      361 gtaagtgcag ttatcctggg tgaaaataca gccgggccac agatcgattt tggggttatt
      421 cccctgggcg ggtcgggctg cactccagcg ccggggcgct ggctcttgcg ggcgtcgctg
      481 cccgaggcag tttatggcaa tggtgtggtc agtgatggca cttatattta ctcgctggga
      541 gggttttcat ctacagcagg tttaagcgac caatttatgc gttttgatcc acagacaaac
      601 cagtggattg atatcccgtc agcaccaacc ctggtgtatg ccggtctggc tgtgtttgcc
      661 gaggggaaaa tttttctgtt tggcggcgtg gacgcctcag cgcccataag tctggttcaa
      721 atttacacga ttgcaaccgg aacctggtca aacggggcaa acatgcctgc accgcgccag
      781 cagatgggcg gcggatatta cgatggcaaa atttatcttg ccggcggatt tgaaagcgac
      841 agcgtaggtt cggcgagcaa tcaaacctgg gaatatgcgc cgtccagtaa cagttgggca
      901 gtcaaagcga caatgccggc cgccctggcc ggaccagggt ctgggatcat tggtggggcc
      961 ttatatcttg ccgggggacg tgacagcgcc gggacgtctt tgaacaccgc ttaccgttat
     1021 gatattgcca gtgatgcctg ggccagtatc gctgcactgc caaacgcggt taattatccg
     1081 ggcagcgcag tgtatggtca aagattatgg attttcggcg ggggcgatcc atttttgaac
     1141 gacgagacag gcgttgaaaa tgagactgaa agcctgagct ttacccagat ctataatccc
     1201 cataccaaca gttggagctc agggcccagc cagaatctag cgcgctcttt ccagggtggg
     1261 gcggttgtgc agaatcagat cttttcagtt ggcggttacc caggcggtat gtggagttca
     1321 aacgtcgttg aagcagccac ccagaacccg ttgaagattc tgatgatcta tgctgataac
     1381 aggccaccca gtgtgctgca atttttattg atgtctcaac ccggagttgg gcaggttgat
     1441 gtcatcagcg caaacagcag cgtcattggt gccgggacca tgatggccta tgatgttgtg
     1501 gtgatcttct ccaacgcctc gtttagtaac gatgtggccc agggtgatca aattgcagat
     1561 tacatcgaca tgggcggggc ggtggtggcg tttaattttt cattttcaag tggataccaa
     1621 ctggctggcc gttggattac cgatggatat accccgttta atttatcaac cggcaacgct
     1681 ttcagcgatg tctctctggg cagctatgtg accggacacc cgttaatggc aggcgtgaat
     1741 tcgttgaacg cctactatca taccaatgta tcgttggcgc ctggcagcgg gttggtggcg
     1801 acctggagcg atggaactct ggcaatggct gtgaacgggc aggctgtggg tgtcaatgcc
     1861 tatgtcggcg aggttcccaa aaactggagc ggcgatttcg cggctctgat tgccaatgcg
     1921 ggatactggc tgcgggacgg gaacggtttt tgcattcatc aggcctgcac cactccagaa
     1981 aacattaccg gagcactgac tgacagcgac tacacgcaaa ccggacgctt ggcacgatat
     2041 gatccggcga gtacctgtgc tgcgccaaca tcctgtccgg ggccctataa ttctacagaa
     2101 acgcactatg acgcctatgc gtttatgaat aacacccccg ccacccagtg tgtgaccatc
     2161 acacttgacc ccaaaggctg tgtggatacc cattaccttc agagcgcggc ctacaatggg
     2221 ttcctgtcta acgatctctg tgccaatt
//
LOCUS       DAOPVS010000253         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_52039_length_2228_cov_52.698573, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000253 DAOPVS010000000
VERSION     DAOPVS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52039_length_2228_cov_52.698573"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKL11_11320"
     CDS             complement(<1..412)
                     /locus_tag="PKL11_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14233.1"
                     /translation="MTPNLTWERLELKLRNPFRLSYGVTDTREAFWIRLAEDAGWGEG
                     TIAPYYKIDPKDMMATWEAAAQKTEPLPNTVADIPEWVGKEGPVTARCAIDLALYDRI
                     GKAQGVPLFRLLGVPRPRPMPTSFTIAIDTPEAMA"
     gene            complement(495..1277)
                     /locus_tag="PKL11_11325"
     CDS             complement(495..1277)
                     /locus_tag="PKL11_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14234.1"
                     /translation="MPGWRTDWSVRMLAAGLMLLILLSGCTPAATELPPTPTSTLTPT
                     TTPTVVWFPATATPSPQPTPQPSPTSEVRPALGEVIFSDGFTSAGDWPVGSLPAGNIA
                     LGDRRLTLAISQAGGTLSALRGQPALQNGYIEITAAPSLCRSADQYGLLFRAASVMDT
                     YRLLLRCDGQARLERLNNGRIKILQDWLPSADIPQGAPGTARIGIWMQGTEIKIFVND
                     AFQFSVTDDGFSSGQMGLFARSGGDTAVSVAFSGLTVWALAP"
     gene            complement(1258..>2228)
                     /locus_tag="PKL11_11330"
     CDS             complement(1258..>2228)
                     /locus_tag="PKL11_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNZ14235.1"
                     /translation="HRALDAAQERARGQLQIGTTGRGIGPAYTDKAARRGLRLLDTLD
                     EATLNERLRNLLVDSNRMLTAWGAEPLDVKAAIDDYLPAALALRPYLTDTGLLLHQAL
                     KAGARVLAEGAQGTLLDLDLGTYPFVTSSSTTTAGVLPGLGLGMLAPGRVIGVTKAFQ
                     TRVGSGPFPTEEQDETMSTRLRGTGANPWDEFGTTTGRPRRVGWLDGVLLRYAVRVNG
                     LTELVLTKLDILSGLPVIRICTAYREAGQRVDELPLGPANLAPFEPVYEEMPGWNEDV
                     SRVRRWQDLPAAARAYVQRVEAISGAAVSLVSVGPEREQIIARLAN"
BASE COUNT          449 a          725 c          627 g          427 t
ORIGIN      
        1 gcgccatcgc ttcgggcgtg tcaatggcaa tggtgaagga ggtcggcatg gggcggggcc
       61 ggggaacccc cagcagccgg aacagcggca cgccttgcgc tttcccaatc cggtcataca
      121 gcgccaggtc aatcgcacag cgcgccgtca cggggccttc tttgcccacc cattccggaa
      181 tatccgccac ggtattgggg agcggctcgg ttttttgggc cgcagcttcc caggtcgcca
      241 tcatgtcttt tgggtcaatt ttataataag gcgcaatcgt accttcgccc cagccagcat
      301 cttcagccag ccgaatccag aacgcttctc gggtatcggt aaccccatag gagagccgaa
      361 acgggttgcg cagcttgagt tcaagacgtt cccaggttaa atttggtgtc atggggaatt
      421 tcctttgcta ttgggggaag gtttaaccga aaatgatgat atcacgattg agatttttgg
      481 gcaagtcaat tttcttatgg cgccagcgcc cacaccgtca atccggagaa ggcaacactg
      541 acagccgtat caccgccaga acgcgcaaac aatcccatct gcccgctgga aaatccatca
      601 tcggtaacgc tgaattgaaa ggcatcattc acaaagatct tgatctcagt gccctgcatc
      661 cagatgccaa tccgggctgt gcctggcgcc ccctgtggaa tatctgcact tggcagccaa
      721 tcctgtagaa ttttaatccg cccattgttc agcctttcca ggcgagcctg cccatcacag
      781 cgcagcagca gccggtaagt atccatcacg gaagccgccc ggaacaacag cccatactga
      841 tccgcgctgc ggcacaggct gggtgcggcg gtgatctcaa tatagccatt ctgcagcgca
      901 ggctgacccc gcagtgcaga aagggttcct cccgcctgag agattgccaa cgtcaagcgg
      961 cggtctccca gggcaatatt accggctggc aggctgccca ccggccaatc gccagcgctg
     1021 gtgaacccat cgctgaaaat gacttcgccc agtgccggcc ggacctcgga tgtcggcgat
     1081 ggctgaggtg tgggctgcgg gctgggagta gcggttgccg ggaaccacac cactgtgggt
     1141 gtggtcgttg gggtcagggt gctggttggt gtgggcggca gttcagttgc cgctggcgtg
     1201 cagccactca gcaggatcaa cagcatcaac cctgctgcca gcatccgcac cgaccaatca
     1261 gttcgccagc cgggcaatga tttgctcccg ttctggcccc accgaaacca gtgagaccgc
     1321 cgccccgctg atcgcttcaa cccgctgcac ataagcccgt gccgccgcgg gtaaatcctg
     1381 ccaccgccgc acccggctca cgtcctcatt ccatccgggc atctcctcat atacaggttc
     1441 aaatggcgcc aggttggctg gccccagggg cagttcatct acgcgctgcc cggcttcgcg
     1501 ataggctgta caaatccgaa tcaccggcag cccgctcaaa atatccagct tggtcaaaac
     1561 cagctcggtc aatccattca cccggacggc atagcgcagc agcacaccat ccagccaacc
     1621 cacgcggcgc gggcgcccgg tggtggtgcc aaactcgtcc cagggattgg cgcccgtccc
     1681 ccgcagccgc gtagacatgg tctcatcctg ttcttcagtc gggaaaggcc cagacccaac
     1741 ccgggtctga aaggccttcg tcacaccaat cacgcgtcct ggcgccagca ttcccaatcc
     1801 caacccgggc agtacacccg ccgtggtggt gctggaactg gtcacaaaag gataagtccc
     1861 caggtccagg tccagcaagg tgccctgggc gccttctgcc agcacacggg cgccagcttt
     1921 caaggcctga tgcagtaaca ggcccgtatc ggtcagatag gggcgcagtg ccagcgccgc
     1981 tggcaaataa tcatcaatgg cggccttcac atctagcggc tcggcccccc aggctgtcaa
     2041 catccggttg gaatccacca gcaggtttcg caggcgctca ttgagggtcg cctcgtccag
     2101 cgtatctagc agccgcagcc cgcgccgggc ggctttgtcg gtataagccg gtccaatccc
     2161 ccgtccagtt gtaccaattt gaagctgccc gcgcgcccgt tcctgggcgg catccagcgc
     2221 ccggtgtg
//
LOCUS       DAOPVS010000254         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_52396_length_2218_cov_47.246879, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000254 DAOPVS010000000
VERSION     DAOPVS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52396_length_2218_cov_47.246879"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKL11_11335"
     CDS             complement(<1..185)
                     /locus_tag="PKL11_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14236.1"
                     /translation="MKPPGDRTAERAAGSNCITRVERVGGVGFGRVGAARQVAPLPLE
                     PRTQTAALRGWFAFLLR"
     gene            189..>2218
                     /locus_tag="PKL11_11340"
     CDS             189..>2218
                     /locus_tag="PKL11_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ14237.1"
                     /translation="MKIRFSPLLALVLILALSLSGAAPAQAQPRAAVPVDPVVQEILD
                     DITQDQIITYTHQLAGDTPIFVDGANYTLTSRNTYSGTDIQKATNFVGQHMAALGLDV
                     EYHTWGGATYPNVIGEIPGLVNPDQIYIIGAHLDDMPSSGRAPGADDNASGSVATLLA
                     ADLLSQYDWGCTIRFAFWTGEEQGLNGSDAYATRSAGLNESIISYLNLDMVAYNTIGS
                     ANGIDLRYNPNITGSQALAQLFADVIDAYDLNLVPDIGTTLAGGSDHESFWDQDYNAI
                     LAIEDQSDFNPYYHTANDTVAHVDPAYYTEFVKASIATLVHESGCLLTEGVGTLSGTV
                     TDAATSAPIPGTTITLQGTSGSPITLTADASGSYTQNLLEDTYTASVWAYGYEPVVVE
                     NIVITADQTTTQDFALTLAETHTLSGTVSDANSSAPLSANITVDGSPETAVTDPATGF
                     YSLELPPGTYTLHVTSAGHQAAAREVLMADADQTQNFSLPPLPPVLLVDDDQNNPNLQ
                     TYYTGALDQISVAYDVWDVAAAGAPSADDLAGYCMVIWYTGQPSSGTFSSSDETAVAA
                     YLDGGGRFFLSSYEYLYERDLTTFGQNYLHIGSYTDDVSLTNPTGVTGDPIGDGLGPY
                     SLTKPTGWTATLWTDNVNKDASAGAGTPFKWDGTGNTNSVRYENATFKTAFIA"
BASE COUNT          454 a          735 c          592 g          437 t
ORIGIN      
        1 ttccttaaca aaaacgcaaa ccacccccgg agggcggcgg tttgcgttct gggttccagg
       61 ggcaatggcg ccacctggcg tgccgcgccc acgcgcccaa aaccaacacc cccaacccgc
      121 tccacgcgcg tgatacaatt agacccggcg gcgcgttctg ccgtcctatc ccccggaggt
      181 ttcatactat gaagattcgt ttttctcccc tgctggcgct ggtgttgatc ctggcgttaa
      241 gcctgtcggg cgcggccccg gcccaggcgc agccccgcgc ggccgtaccg gttgacccgg
      301 tggttcaaga gattctggat gatattaccc aggatcagat catcacctac acccaccagt
      361 tggctggcga cacccccatc tttgtggatg gtgcaaacta taccctgacc tcccgcaata
      421 cgtacagcgg aactgacatt caaaaggcca ccaattttgt tggtcagcat atggctgccc
      481 tgggcctgga tgtggaatat cacacctggg gcggggccac ctaccccaac gtgattggcg
      541 agatccccgg cctggtcaat cccgatcaga tttacatcat cggcgcgcac ctggatgata
      601 tgcccagctc tggccgggcc cccggcgccg atgataatgc cagcggttcg gtagccaccc
      661 tgctggctgc cgacctgctc tcgcagtacg attggggctg caccatccgc tttgccttct
      721 ggaccggtga ggaacagggg ctgaacggca gcgatgctta tgccacccgc tccgccgggc
      781 taaacgagag catcatctcc tatcttaatc tggacatggt tgcttacaat accattggct
      841 ctgccaacgg aattgatctg cgctacaatc ccaatatcac tggcagccag gcgctggcgc
      901 agcttttcgc cgatgtgatt gatgcctatg acctgaacct ggtgcccgat attggcacca
      961 ccctggctgg cggcagtgat cacgaatcat tctgggatca ggattacaat gccattctgg
     1021 cgattgaaga tcaatctgac ttcaacccct attatcatac cgccaatgat accgtggcgc
     1081 atgttgatcc ggcctactat accgaatttg tgaaggcctc catcgccacc cttgtgcatg
     1141 aaagcggctg tctgctgacc gaaggcgtgg gcaccctcag cggcaccgtg accgatgccg
     1201 ccaccagcgc gcccatcccc ggcacaacca tcaccctgca gggcaccagc ggctcaccca
     1261 tcacgctcac cgcagatgcc agcggcagtt acacccagaa cctgctggaa gatacctaca
     1321 ccgccagcgt ctgggcttat ggctacgagc cggtggtggt tgaaaacatc gtcatcaccg
     1381 ccgaccagac caccacgcag gattttgccc tgaccctggc cgagacgcat acgctctcgg
     1441 gcacggtgag cgatgcgaac agcagcgcgc cgctgtctgc caacatcacc gtggatggct
     1501 cgcccgaaac ggccgtcacc gacccggcga cgggctttta cagcctggaa ctgccgcccg
     1561 gcacctacac cctgcatgtg acctcggccg ggcatcaggc tgccgcgcgt gaggttctga
     1621 tggctgacgc cgaccagacc caaaattttt ccttaccccc gctcccgccc gtattactgg
     1681 tggacgacga ccagaacaac cccaacctgc aaacctacta cacgggcgcc ctcgatcaga
     1741 tcagtgtagc gtacgacgtg tgggacgtgg ctgccgcggg ggctccttcc gctgatgacc
     1801 tggccgggta ctgcatggtg atctggtaca ccgggcagcc ctcttcaggc accttctcca
     1861 gcagcgatga aacggcagtg gcggcctacc tggatggcgg cgggcgtttc ttcctctcta
     1921 gctatgagta cctttacgag cgcgacctga ccacgtttgg gcagaactac ctgcatattg
     1981 gcagttacac tgatgatgtg tcgctcacca atccaaccgg cgtgactggc gaccccatcg
     2041 gcgatggtct gggcccgtac tcattaacca aacccaccgg ctggacagcc accctgtgga
     2101 cagataacgt taacaaagat gcctctgccg gcgccggcac accgttcaaa tgggacggaa
     2161 ctggcaatac caacagtgtg cgctatgaaa atgccacctt caagacggcc ttcatcgc
//
LOCUS       DAOPVS010000255         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_52500_length_2215_cov_42.475926, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000255 DAOPVS010000000
VERSION     DAOPVS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52500_length_2215_cov_42.475926"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKL11_11345"
     CDS             complement(<1..494)
                     /locus_tag="PKL11_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14238.1"
                     /translation="MKPIHFSLRTVLLAAALLLLIPGVTVLAQEIITSAEPGYVSPEA
                     AFAPNPADPDYIDPALNAADPNVTYYYTFVAGSTLRPRDSTADWHIDSSGGCLYTNAS
                     GEIFNVDLQLPDGATIEYLRLYYYDTSASDSRAWVTSYDGAGNYDDLITVASTGDTGY
                     GNTL"
     gene            complement(507..1106)
                     /locus_tag="PKL11_11350"
     CDS             complement(507..1106)
                     /locus_tag="PKL11_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14239.1"
                     /translation="MKLKRWQFILSFALLAGLLISAVGSALANNTAPAAAPALVRSEN
                     SVLAPHPYYYLFVPGTALRPRESGAPWAADGGAGCIYSTTSSGVVFNVDIQLPEGAGI
                     DYLRIFYNDTSASDSIAWVTSYNGAGGYDDLVNVSSTGSSGFGTSLSDFTRVIVHNAN
                     VPLVLNWRPNVAGSTMQLCGLRIAYHMPDYYTYLPSILR"
     gene            complement(1139..1903)
                     /locus_tag="PKL11_11355"
     CDS             complement(1139..1903)
                     /locus_tag="PKL11_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14240.1"
                     /translation="MKHVRFSLRTALILAALLLIVPGITVLAQEIISSSEPGYTAPVQ
                     ALPEQIIGDDPDEDGYTGPITMADPNADIPHSGDGAEVDLSQFETDGEPQPDEDAYYD
                     QPGVNATVDAGWSHFYYMYVAGATLRQRVSGNNWHTDGSGGCVYTDNTFNILKLDAQF
                     PEGARIDYLRIYYYDTSASDSTAWVTSYNGAGDLTNLTNVSSFDDTGYGTNLGAYLGH
                     VVDNFTNPYVLNWQPWVADNTMQLCGLRVAYRLPTP"
BASE COUNT          501 a          579 c          662 g          473 t
ORIGIN      
        1 ctgagcgtgt tgccatagcc ggtgtcgcca gtggaggcaa cggtaatcaa atcatcatag
       61 tttcctgcac catcgtaaga agtcacccag gcgcggctgt cgctcgccga ggtgtcgtag
      121 taatagaggc gcaggtactc aatggtggcg ccatcgggca gctgcaaatc gacattgaag
      181 atttcacctg acgcatttgt atagaggcag ccgccgcttg aatcaatgtg ccagtcagca
      241 gtactgtcgc gcggccgcag ggtggaaccc gccacaaagg tgtaataata ggtgacgttg
      301 ggatcggcag cattgagggc cgggtcaata taatcggggt cggcggggtt gggggcaaaa
      361 gccgcctcgg ggctcacata gcctggctcg gcgctggtga tgatctcttg agccagcacg
      421 gtcacgccgg ggatgagcag cagcagggcg gcggcgagca aaacagtacg cagtgagaaa
      481 tgaatgggtt tcatggggtc atctccttag cgcagaatgc tgggcagata ggtgtagtaa
      541 tctggcatgt gataggcgat ccgcagcccg caaagctgca tggtgctgcc agccacattg
      601 ggccgccagt tcagcaccag cggcacattg gcgttatgca caatcacgcg tgtgaagtcg
      661 ctcaaactgg tgccgaagcc gctggagccg gtggaactga cattgacaag gtcatcatat
      721 ccacctgcgc cattgtaaga ggtcacccag gcaatagaat ctgaggccga ggtgtcatta
      781 tagaatatgc gcagataatc aatgccagcc ccttccggaa gctgtatatc tacattgaaa
      841 acgacgccgc tgctggttgt tgaatagata cagccggcgc cgccatcagc ggcccagggg
      901 gcgccgcttt cgcgagggcg cagggctgta cctggcacaa acaagtaata gtaggggtgc
      961 ggcgccagca cactgttctc cgagcgcacc agggcagggg cggctgcggg ggcggtgttg
     1021 ttggccaggg cgctgcccac tgccgaaatc aacagaccgg ccagcagggc aaagctcaaa
     1081 atgaactgcc atcttttaag tttcatgagg ttgctccttc aaattctgtt gatcggaatc
     1141 agggtgtggg caggcggtag gccacccgca aaccgcaaag ctgcatcgta ttatcggcaa
     1201 cccacggctg ccagttcagc acgtaggggt tagtaaagtt atccaccaca tggcccagat
     1261 aggcgcccaa attggttccg taaccggtat cgtcgaagga cgaaacattg gtcaggttgg
     1321 tcaggtcgcc tgccccgtta tacgaggtga cccaggcggt gctgtcgctg gctgaagtgt
     1381 catagtaata gatccgcagg tagtcaatcc gggcgccttc agggaactgg gcgtccagtt
     1441 tgaggatatt aaacgtatta tccgtgtaca cacagccgcc actgccatca gtgtgccagt
     1501 tattaccgct gacgcgctgc cgcagggtgg ccccggcaac atacatgtag tagaagtggc
     1561 tccagcccgc gtcgacggtg gcgttcacgc cgggctggtc gtagtaggcg tcttcatcgg
     1621 gctggggttc gccatcggtt tcaaactgcg aaaggtccac ctccgcccca tcgccgctgt
     1681 gagggatatc agcattgggg tcagccatgg tgatgggccc ggtgtaacca tcttcatccg
     1741 ggtcgtcgcc gatgatctgc tcaggcaggg cctgcaccgg ggccgtatag cccggctcag
     1801 aactgctgat gatctcttgc gccagcaccg taatgcccgg aacaatcagc agcagggctg
     1861 ccagaatgag cgcggtgcgc agcgaaaaac ggacgtgctt cataaatctt cctccttcgg
     1921 ccccggaggg gccaaaacga aacgaagtat tcaaagaatg agcagggggc aaacgcccgc
     1981 tgctcattaa tctaagatta ataatacatc aattttatca agaatacaat cccttatctc
     2041 gggtattgag gtaattattg tcttatttat atcgttttag cgcccccttt ttggggcggc
     2101 aggttgaatt ctggttgaat gcgttgttta ggtaccctgc ccacccgaat tcttggccct
     2161 gctttgcttg acgggttgta tcaattaaga tacgatactc cttatggacg aactg
//
LOCUS       DAOPVS010000256         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_52660_length_2211_cov_57.993506, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000256 DAOPVS010000000
VERSION     DAOPVS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52660_length_2211_cov_57.993506"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PKL11_11360"
     CDS             <1..1064
                     /locus_tag="PKL11_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ14241.1"
                     /translation="RGAGLGPAITFLFVGPAINILAITYTGATIGMDIAIARLVLSIA
                     FGIIIGLIMAWIFRKEETAREAEMTASGVFDQTARVKPAVWVLFLLLVAVLIVGTLQV
                     GLFTKVYASIHLPFDPASGFVAFLSTLNLTVQGALLILMLVVIGITAWQGFEKIFDGF
                     NAWTYIAFAMIALTILIAAPKEDIGSLVIGINGRFIGELVLLAAIGVVAGRSFTRDEL
                     AGWIFETWKFVKQIFPLLIVGVFLAGIVKVIIPEAWVRTIAGQNTLWANLVGVIFGVF
                     MYFPTLVEVPVAKMFLDLGMARGPLLAYLLADPELSLQSILVLNGVMGKKKTAIYVGL
                     VALFSTLAGYLFGLAIAGL"
     gene            1118..1501
                     /locus_tag="PKL11_11365"
     CDS             1118..1501
                     /locus_tag="PKL11_11365"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HNZ14242.1"
                     /translation="MDCCSTSCSSSNTPTHNPEIGKTAERLAALLKQTAEYQEFVRLA
                     SLVNQDPDVRRVLLEIRQQQMMYSDPAGASVEALQAELETLPAVQAYRSAEAAVKTLF
                     QTVDRAIGAAAGVEFAPNALRSGCG"
     gene            1571..1930
                     /locus_tag="PKL11_11370"
     CDS             1571..1930
                     /locus_tag="PKL11_11370"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HNZ14243.1"
                     /translation="MELMAEITQTAQELGALLGAEASVNTYLTLVETVQQNAETAALE
                     ARFDQLFQKLAEQEQLGQPLDRGELDEYYALKRQVQAHPLIIERNDQHTAIQALFAET
                     AQRMTTILGFDYTAFAR"
     gene            complement(1979..>2211)
                     /locus_tag="PKL11_11375"
     CDS             complement(1979..>2211)
                     /locus_tag="PKL11_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14244.1"
                     /translation="KKTTTLSFTAASNNFELAIAVAVAVFGINSSEAFTAVIGPLVEV
                     PVMIGLVNVAFWLQRKYFGQQVEASKPGPDLG"
BASE COUNT          447 a          615 c          637 g          512 t
ORIGIN      
        1 agcgcggcgc cgggcttggc ccggcgatca ccttcctgtt tgtgggcccg gcgatcaaca
       61 ttctggccat cacctacact ggcgcaacca tcgggatgga tattgccatc gcgcgcctgg
      121 tcttatccat cgcctttggc atcatcattg gtctgatcat ggcctggatc ttcagaaaag
      181 aggaaaccgc gcgcgaggct gaaatgacgg ccagcggcgt atttgatcaa acagccaggg
      241 tgaagccagc cgtgtgggtg ctgtttcttt tactggtggc ggtattgatt gtcggcaccc
      301 tgcaggtggg tctgttcaca aaggtatatg ccagcatcca tttgcccttt gacccggcaa
      361 gcggcttcgt tgccttctta tcaacgctga acttaaccgt tcagggcgct ctgctgattc
      421 tgatgctggt ggtcattggc attaccgcct ggcaaggctt tgagaagatt tttgacggct
      481 tcaatgcctg gacatatatc gcctttgcaa tgattgcgct gacgatcttg attgccgcac
      541 cgaaagagga catcggctcg ctggtgattg gcatcaacgg gcgcttcatc ggtgagctgg
      601 ttttgctggc tgccattggg gttgtggctg gccgatcatt cacccgggat gaactggcgg
      661 gctggatttt tgagacctgg aagttcgtca agcaaatctt cccgctgctg attgtgggcg
      721 tctttcttgc cggaatcgtc aaggtcatca ttcccgaagc ctgggtgcgt accattgccg
      781 ggcaaaacac cctctgggcc aacctggtcg gtgtgatctt tggggtgttt atgtactttc
      841 ccaccctggt ggaagtgccg gtggctaaaa tgttcctgga cctgggcatg gcccgcggcc
      901 cgctgctggc ttacctgctg gctgaccccg aactatcgct gcaatccatt ctggtgctga
      961 acggggtgat ggggaagaag aaaaccgcca tttatgtcgg gctggtggct ctattcagca
     1021 ccctcgccgg atacctgttt ggcctggcga ttgcagggtt ataatggggc tggcgcaagc
     1081 cgcccgtaga tcatcattga ctgaaaggat tttgcgaatg gattgttgtt ctacatcttg
     1141 ctcttcatca aatacaccca cccataaccc ggaaattgga aaaaccgccg aacgcctggc
     1201 tgcgctgctc aagcagactg ctgaatatca ggagtttgtg cgcctggcca gcctggtcaa
     1261 ccaggacccc gatgtccggc gcgttttatt ggaaatccgc cagcagcaaa tgatgtactc
     1321 ggacccggcg ggggcctctg ttgaagccct gcaggccgag ctggagaccc tgcccgctgt
     1381 gcaggcctac cgctctgccg aggctgccgt taaaacgctg ttccagacgg tggaccgggc
     1441 cattggcgca gctgccgggg tagaatttgc ccccaacgcg ctgcgcagcg gctgtggctg
     1501 agggggttga aattgggtca ggtctgaccc acccacctca aaccaccgaa ttaatcagga
     1561 gtacatcatc atggaattaa tggctgagat tacacaaacc gctcaagagc tgggcgccct
     1621 gctgggcgct gaagccagcg tcaatactta tttgactctg gtcgagaccg tgcagcagaa
     1681 tgccgaaacg gccgcgctgg aggcccgctt cgatcaattg tttcaaaaac tggcggagca
     1741 ggaacagctt ggccagcccc tggaccgggg agaactggac gaatattacg ccttgaaacg
     1801 tcaggtgcag gcgcacccgt tgattattga acgcaatgat cagcacacgg ccatccaggc
     1861 cctgtttgcc gaaaccgcgc agcggatgac aacgattttg gggtttgatt acaccgcttt
     1921 tgcacgttga acccatcaaa cggggcggga gtagtttttc ttccgccccg tagagttgct
     1981 aaccgagatc cggcccaggc ttgctggcct caacctgctg cccaaagtac ttgcgctgca
     2041 gccagaaagc cacattcacc agcccgatca tcactggcac ctctaccagc gggccaatca
     2101 ccgcggtaaa ggcctcgcta gaattgatgc cgaacaccgc caccgccacg gcaatcgcca
     2161 gctcaaagtt gttgctggcg gctgtgaatg agagcgtggt ggtctttttg t
//
LOCUS       DAOPVS010000257         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_52819_length_2207_cov_72.678439, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000257 DAOPVS010000000
VERSION     DAOPVS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52819_length_2207_cov_72.678439"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PKL11_11380"
     CDS             complement(<1..1295)
                     /locus_tag="PKL11_11380"
                     /inference="COORDINATES: protein motif:HMM:NF006385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HNZ14245.1"
                     /translation="MLINHTVTQTMRFLTHRYLNLQPTPLRQVPPPQPNRAYMLYAHV
                     PFCESLCPYCSFNRFLFQEGRARAYFQQLRAEMRLVASLGYRFQTLYIGGGTPTVLVD
                     ELIQTIELAHQLFPLTEVSCETNPNHMTLENARRLSPYIQRMSVGVQSFNDHQLKHML
                     RYDRFGSGADILRQLALVQNQFKFLNIDLIFNLPGQTEAQLLDDVAQACRSGANQITF
                     YPLMSSPSVSQAMRKTLGQPSYTNERRYYDLIVSAMQDRFQLSTVWNFGQADPSLPAE
                     DAMIDEYIINYAEYVGLGSGSFSYLDGHLYVNTFSVNEYARQVEGGQMALTAQASFSR
                     REQMLYRFMMDLFGLRLDKAKFRQEFGTAVDAALAPEIALLALAGAFEQNDRTALTLS
                     ARGRYLLLIMMREFFVGMNTVRDQARAALPESERLQLCAT"
     gene            1582..>2207
                     /gene="ygiD"
                     /locus_tag="PKL11_11385"
     CDS             1582..>2207
                     /gene="ygiD"
                     /locus_tag="PKL11_11385"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNZ14246.1"
                     /translation="MPEESRMPALFVGHGSPVNAIEDNPYSQTWRSLGRSLPRPRAIL
                     CISAHWETPQTEVTAMPQPRTIHDFGGFPNALYEAKYPAPGSPWLAEAVQQTLAPLPV
                     GLNQDWGLDHGCWSVLMQMYPQADIPVAQLSLDYTQPPQYHYDLGRKLAPLREQGVLI
                     LGSGNIVHNLRRVVVPGNNYERFNEPFGLDWALEAQALFKKLIDADDHA"
BASE COUNT          479 a          570 c          721 g          437 t
ORIGIN      
        1 gtggcgcaaa gctgcaggcg ttcagattcc ggcagggcgg cgcgggcctg gtctcggacg
       61 gtgttcatgc ccacgaagaa ttcacgcatc atgatcagca gcaggtagcg cccgcgggcg
      121 ctgagggtga gggcggtgcg gtcattctgt tcaaaggcgc cggccagtgc cagcagggca
      181 atttcgggcg ccagggcagc atccaccgcc gtgccgaact cctgccggaa tttggccttg
      241 tcgagccgca ggccaaagag gtccatcatg aagcggtaga gcatctgttc gcggcggctg
      301 aaggaggcct gggccgtcag ggccatctgt ccgccttcca cctggcgggc atactcattg
      361 accgagaagg tgttgacata caggtggcca tccaggtagc tgaacgagcc ggagccaagg
      421 cccacatatt cggcataatt gatgatgtat tcatctatca tggcgtcctc tgcgggcaag
      481 gaggggtcgg cctggccaaa attccagacg gttgaaagct gaaaccgatc ctgcatggcg
      541 gagacaatca ggtcgtaata gcggcgctcg ttggtgtagc tgggctgccc cagggttttg
      601 cgcatggcct gtgaaaccga gggggaactc atcagcgggt aaaaggtgat ctggttggcg
      661 ccgctgcggc aggcctgagc cacgtcgtcg agcagttggg cttcggtttg gcccggcagg
      721 ttgaagatca ggtcaatgtt taaaaatttg aactgattct gcaccagggc taactggcgc
      781 aagatatcgg cgccggagcc aaagcggtca taacgcagca tgtgttttag ctggtgatcg
      841 ttaaagctct gcacccccac cgacatgcgt tgaatatagg ggctgagccg gcgggcattt
      901 tcgagcgtca tatgattggg gttggtctcg caggagactt cggtcagcgg gaagagctgg
      961 tgtgccagtt cgatggtctg gatcagttca tccaccagca cggtgggggt gccgccgccg
     1021 atatagaggg tttgaaaacg gtagcccagg ctggctacca ggcgcatttc ggcccgcaat
     1081 tgctggaagt aggcccgggc gcggccctcc tgaaagagaa agcgattaaa ggaacagtag
     1141 gggcagaggc tctcgcagaa cggcacatgg gcatacagca tataggcgcg gttgggctgc
     1201 gggggcggca cctgccgcag cggggtgggc tgcaagttga gatagcggtg cgtcaaaaag
     1261 cgcatggtct gggtgacggt gtgattgatc agcatagggc ggcatccttg gggcagcagg
     1321 cagagaaaag agactgccgg gttcagacag tctctgcgcg cagggcattc ttccggcagg
     1381 cagtgacaaa tcggcaaaaa ccggtcaaat ttgacaaatt ttattcaaat tacagacttt
     1441 aatataccac caatgatgcg ggtgtgaagc atcaatccaa actgcttaca aaaccgataa
     1501 gctgccgggg cagggcagga tttttgataa aatcgatttg atttataatg taaactaacc
     1561 cttgaccctg gaggtgctac catgccagaa gaatcccgaa tgccggcttt gtttgtgggg
     1621 cacggcagcc cagtgaatgc aattgaagat aacccctatt cgcaaacctg gcggagcctg
     1681 gggcgcagcc tgccccggcc cagggccatt ctgtgcatct cggcgcactg ggagacgccc
     1741 cagaccgaag tgaccgccat gccgcagccg cgcaccattc acgattttgg cgggttcccc
     1801 aatgcacttt atgaggccaa gtacccggcg ccgggcagcc cctggctggc agaggctgtg
     1861 cagcaaaccc tggcgcccct gcccgtgggc ttaaaccagg actggggact ggatcacggc
     1921 tgctggagtg tgttgatgca gatgtacccg caggccgata ttcccgtagc gcagttgagc
     1981 ctggattaca cccagccgcc gcaatatcat tatgacctgg ggcgcaaact ggcgccgctg
     2041 cgggagcagg gggtgctgat tctgggcagc ggcaatattg tgcataacct gcggcgggtg
     2101 gtggtgccgg ggaataatta cgagcgcttc aacgagccgt ttgggttgga ttgggcgctg
     2161 gaagctcagg cactgtttaa aaagctgatc gatgccgatg accatgc
//
LOCUS       DAOPVS010000258         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_52852_length_2206_cov_57.716411, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000258 DAOPVS010000000
VERSION     DAOPVS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52852_length_2206_cov_57.716411"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKL11_11390"
     CDS             complement(<1..615)
                     /locus_tag="PKL11_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNZ14247.1"
                     /translation="MEFEFLPLSGADNASGAVVVIDVVRAFTTAAYAFAAGASAITVT
                     GEISAALALQQSIPGARLMGENDGNLIPGFDYGNSPEQVQAQDLRQVHLIQRTSAGTQ
                     GLVRAVHASTLLAASLVVASATAEHLRRLSPPRVTFIATGLGADGWGDEDLACAEYIA
                     ALLRGESPDIPALQQRIRRSPVGQRMVAPGQTTFWPGDLTLCLAV"
     gene            complement(623..2173)
                     /locus_tag="PKL11_11395"
     CDS             complement(623..2173)
                     /locus_tag="PKL11_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNZ14248.1"
                     /translation="MTLLSVLVALLPIILIFLLLTVRRTAADIAGLIGWVATVLVAWL
                     YFQTPLQVTFLASLSGLFASLPIALVVATSIFQITVMMETGAMARVVALLKTIAPRDQ
                     VVQIMLINVGAGTLLAALGSTPVSILPPILLALGYTPFVAIALPAIGYDALCTYALLG
                     TPVVVFSNFVGLPVTEVGGYFARYMPIISTCIAFGMLWIVGRWKLVWKGLVPTLIAGL
                     TAGLIAIGMNAAGLVTLTGVAAGLGVILVMLAYLKLTGRPILDRSVQTDADRVAETRL
                     SLWAALSPWLILTLFSLIINTPALPFFDLLFKQLAMPLEIIPGAPEKLRVFWQAYFWI
                     LISTLIALPFMKTTRSQLAASLRKWSKRAPRPMLAAGVFFAIAYVINHSGKTLDWQLV
                     DPNLNMVQLLAGAAADGFGRLYPLVSPFLGLLAGFISGSEASALAMLTQINLSTAERI
                     GAIGLLVAASSGIGGGLASVISPAKLQNAAASIDRIGEESRVIFTAFVISIVITIIAA
                     VLTLIWAF"
BASE COUNT          560 a          595 c          728 g          323 t
ORIGIN      
        1 gactgccagg cagagggtta gatcgccggg ccagaaggtg gtctggccgg gggcgaccat
       61 ccgctgcccc accggggaac ggcgaatgcg ctgctgaagc gcaggaatat caggggattc
      121 accgcgcagc agtgcggcga tatattcagc acaggccagg tcttcatcgc cccagccatc
      181 agcgcccagg ccagtggcga taaacgttac gcggggcgga gacaggcggc ggagatgctc
      241 tgcagtggcg gaggcaacca ccaggctggc agccagcaag gtgctggcat ggaccgcgcg
      301 caccaggccc tgggtgcccg ccgaggtgcg ttgaatcaga tgcacctggc gcagatcttg
      361 agcctgcacc tgctcgggcg agttgccata atcaaaaccg gggatgaggt tgccatcatt
      421 ctcgcccatc aggcgagcgc cggggatgga ctgctgcaag gccagggcgg cgctaatctc
      481 accggtgacg gtgatggcag atgcgccagc ggcgaaggca taggcggcgg tagtaaaggc
      541 gcggacaacg tcaatgacca ctaccgcgcc cgaggcatta tcggcgccag agaggggtaa
      601 aaactcaaat tccatcagaa cctcagaaag cccagatgag ggtcagtaca gcggctatga
      661 tggtgatgac aatggagatc acaaaagctg taaaaatcac ccgcgactct tcgccaatgc
      721 ggtcaatgct ggcggcagca ttttgcagct tggccgggga gatgaccgag gccagaccgc
      781 cgccaatacc gctggaggca gccaccagca atccaatagc gccaatgcgc tcggcggtgg
      841 acaggttgat ctgcgtcaac atggccaggg ccgaagcctc ggaaccgctg ataaacccgg
      901 ccagcagacc gagaaacggt gaaaccaagg gatagagccg cccaaagcca tcagcggcgg
      961 cgcctgccag caactgcacc atgttcaggt tggggtccac aagctgccaa tcgagggttt
     1021 tgccggaatg attgatgaca taggcaatgg caaagaaaac gccagccgcc agcatggggc
     1081 gcggggcacg tttggaccac ttacgcagag aggctgccaa ctgcgaccgg gtggttttca
     1141 taaagggcag ggcaatcagt gtgctgatca ggatccagaa ataggcctgc cagaagacgc
     1201 gcagtttttc gggtgccccg gggataatct ccaggggcat ggctaactgc ttgaagagca
     1261 gatcaaagaa tggtagggca ggggtattga ttatgaggga aaagagcgtc aaaatgagcc
     1321 agggggagag cgccgcccag agcgagaggc gggtttcggc gacgcggtca gcgtcggttt
     1381 ggaccgagcg atccaggatg gggcgaccag tgagcttgag ataggccagc atcaccagaa
     1441 tgactcccaa tccggcagcc acacccgtta gagtgaccag gccggcagca ttcatgccga
     1501 tggcaatcag gccagcggta agcccggcaa tgagggtggg aaccaggccc ttccagacga
     1561 gcttccagcg gccaacaatc cacaacatgc cgaaggcgat acaggtgctg atgatgggca
     1621 tgtagcgggc aaagtagccg ccgacttcgg tgaccggcaa acccacgaaa ttggagaaaa
     1681 ccaccaccgg cgtgcccagc agggcgtagg tacagagggc atcatagccg atggcgggca
     1741 gggcaatggc cacaaagggg gtataaccta aggccagcaa gatgggcggt aaaatggaga
     1801 ccggggtgga acccaatgca gccagcaaag tgcccgcgcc gacattgatc agcataatct
     1861 ggacaacctg atcgcgcggg gcgatggtct tgagaagtgc aaccacgcga gccattgcgc
     1921 ctgtttccat catgaccgtg atctggaaga tggaggtggc gaccaccagg gcaatgggca
     1981 gcgaggcgaa caagccggac agactggcca ggaatgtgac ctggagcggc gtctggaagt
     2041 agagccaggc caccaatacc gtagccaccc agccaatcag accggcaata tcggcagcgg
     2101 tgcggcggac ggtcagcagc agaaaaatca aaatgatggg caataaggcc actaaaacgg
     2161 aaagcagggt catgaagttg tccttaaccg caggatgggc tgatta
//
LOCUS       DAOPVS010000259         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_53613_length_2186_cov_28.955889, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000259 DAOPVS010000000
VERSION     DAOPVS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53613_length_2186_cov_28.955889"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1071)
                     /gene="ccmA"
                     /locus_tag="PKL11_11400"
     CDS             complement(169..1071)
                     /gene="ccmA"
                     /locus_tag="PKL11_11400"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNZ14249.1"
                     /translation="MNPVILAEHLTRRFGDFVAVDDVSFAVEAGQVVGCLGPNGSGKT
                     TTIRMLLGLLRPSAGAARVLGFDCVSQAEALRARVGYMSQKFALYQELTARENLAFYA
                     GLYGVTDRSRVDEILAELELTPAADQPAQALSAGWKQRLALATALVHRPSLLILDEPT
                     SGVDPVARRTFWDLIYDRAAQGITVLVTTHYMDEAEYCERLAVMRSGRLLALDTPAAL
                     KAGLPGPVWQTAAAPLDTALAALRALPGVLRVGLDSDTLRVITRPEVTAADLQRALTA
                     AGASASQVAPAAPTLEDVFLMLAG"
     gene            complement(1068..2003)
                     /locus_tag="PKL11_11405"
     CDS             complement(1068..2003)
                     /locus_tag="PKL11_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ14250.1"
                     /translation="MALLEATDLHKSFGETHALAGVNLTLAAGEIHGLAGPDGAGKTT
                     LLRLLCGALRPDSGSVTVCGEPLAHRPEQARAHLGYLSQRFSLYEDLTVLENLRFFAE
                     LRGLPTARWLPRSEWALNFVGLAPFSGRLAGQLSGGMKQKLGLATALIAAPEILLLDE
                     PTTGVDPVTRQDFWQLILQLVQTEHIGVLLSTPYMDEASRCHRVSFLRRGQLIASDTP
                     AALRARLAGQVVELRGEPVGALLGLARADADVTSARRFGDALRLGVRPGTAAAVCVRL
                     SQSIPAGGGALAAMAPAEPLLEDVFIYYATLEAQP"
BASE COUNT          410 a          771 c          703 g          302 t
ORIGIN      
        1 gccaaagtga tcaaagaatg gaacaccaaa ctgcaggctg ccggggcagt cgtcacaccc
       61 acggcagaga ttgcgtttgg ataaatgctc ccggtgtggt aacgagaggc gcccgcggca
      121 ccccgcgccc aacaatccta aatcataaat ccaaaatctt aaatcccctc atccagccag
      181 catcaaaaac acgtcttcca gggtgggggc ggcgggggca acctggctgg cagaggcccc
      241 ggcagccgtg agggcgcgct gcaggtcggc ggcggtgacc tcggggcggg tgatgacccg
      301 cagcgtatca ctgtccagcc ccacgcgcag cacccccggc agggcgcgca gcgccgccag
      361 cgccgtatcc agcggcgccg cggccgtctg ccagaccggc cccggcagcc cggccttcag
      421 cgccgcgggc gtatccagcg ccagcagccg cccggagcgc atcaccgcca ggcgctcgca
      481 gtactcggcc tcgtccatgt agtgagtggt caccagcacg gtgatgccct gcgccgcccg
      541 gtcgtaaatc agatcccaga aggtgcggcg cgccaccggg tctaccccac tggtcggctc
      601 atccagaatc aacaggctgg ggcggtgcac cagagcagtg gccagcgcca ggcgctgctt
      661 ccaacccgct gaaagcgcct gggcaggctg gtcggcggcg ggagtcagct ccagctcggc
      721 caaaatctca tccacccggc tgcgatcggt cacgccgtac agcccggcgt agaaggccag
      781 gttttcgcgg gcggtcaatt cctgatagag cgcaaatttc tgcgacatat agcccacccg
      841 ggcgcgcagc gcctcggcct gagagacgca gtcaaacccc agcacccgcg ccgccccggc
      901 gctgggtcgc agcagcccca gcagcatccg gatggtggtg gttttgccgg agccgttggg
      961 ccccaggcag cccaccacct gcccggcctc caccgcaaac gagacatcat ccacggccac
     1021 aaagtcgcca aagcggcggg tcagatgctc ggcaaggatc acggggttca tggctgggcc
     1081 tccagggtgg cgtagtaaat gaagacatct tccaacagcg gctcggcggg ggccatagcc
     1141 gccagcgcgc caccccccgc ggggatgctc tggctgaggc gcacacacac ggccgcggcc
     1201 gtgccggggc gcacccccag gcgcagggca tcgccaaagc gccgggcgct ggtcacatca
     1261 gcatcggcgc gagccagccc cagcagcgcc cccaccggct cgccgcgcag ctccaccacc
     1321 tgcccggcca ggcgcgcccg cagcgcagcc ggtgtatctg aagcaatcaa ctggccgcgg
     1381 cgcaaaaaac tcacccggtg acagcggctg gcctcgtcca tatagggcgt gctgagcagc
     1441 accccgatat gttcggtctg caccaactgc aaaatgagct gccagaaatc ctggcgggtc
     1501 acggggtcaa ccccggtggt gggttcatcc agcaacagta tctccggtgc ggcaatcaat
     1561 gcggtagcca gccccagctt ctgcttcatg ccccccgaaa gctgccccgc cagtcgtccg
     1621 ctgaacggcg ccagccccac aaaattgagc gcccattctg agcgcggcag ccagcgggcg
     1681 gtgggcaggc cgcgcagttc ggcaaagaag cgcagatttt ccagcacggt caggtcttcg
     1741 tagagcgaga accgctgcga aaggtagccc aggtgcgccc gggcctgctc cgggcggtgg
     1801 gccagcggct cgccgcagac ggtcaccgat ccggagtcgg ggcgcagcgc gccgcacaac
     1861 agccgcagca gggtggtctt gccggcgcca tcgggcccgg ccagcccgtg aatctcgccc
     1921 gcagcgagcg tcagattcac acctgccagg gcgtgcgtct cgccaaaaga tttatgcaga
     1981 tcggtggctt ccagcagcgc catggggatg atcctctact cgcccagccg cacatcggcg
     2041 ggcatgccgg gcttcagccg cccgtcggag ttgggcagcg ccaaccggac ggcatagacc
     2101 gtgttgcggc ggctctgcgc ggtttgcacg ttgcgcggcg taaattcggc tgaatcggcg
     2161 atatagacca cctcgccagt aaaggt
//
LOCUS       DAOPVS010000260         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_53657_length_2185_cov_47.037089, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000260 DAOPVS010000000
VERSION     DAOPVS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53657_length_2185_cov_47.037089"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKL11_11410"
     CDS             <1..362
                     /locus_tag="PKL11_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HNZ14251.1"
                     /translation="TEAEDLADQIAIIQRGRIIFNDTSANLKRRLLGAPEYEARLAGI
                     VNGWEPTNIPGVSIVQRTSDVLRFRVEDPQSANPLLLEQMIAHRLPVVSFHEVHRSLE
                     DAYLEAVRQAAAEVPHA"
     gene            355..1773
                     /locus_tag="PKL11_11415"
     CDS             355..1773
                     /locus_tag="PKL11_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNZ14252.1"
                     /translation="MLKSLYPVYVIAHREIRDQFRDWRIVFPIIILTILFPFLMNYTA
                     QEMLGFVGKYGGEIVGERLIPFLLMIVGFFPSSFSLIIALETFVGEKERGSIEPLLST
                     PLEDWQLYLGKLSSAVVPPLFGSFLGMTVYLTGLAIGRAPLPDGEMMTLIILLTIVQA
                     VMMVSGAVVVSCQATSVRAANLLSSFIIIPVTLLIQGESMLMFWGNYGTLWMAFFGVI
                     LLTFLLMRMGLAYFRREELLGREIDVLNLRWGWMTFWQAFTGGAKNVWQWYRRVIPKT
                     LAALRLPFVIVFLMGAAGVAIGFSLVRQFPITAPPEMFTNLEERLGLLTEAFKPGTFG
                     PVLMVFGNNLRVMALALLLGIFSFAVLGVLPIIVTLAVVGYILGILGLNGLPAGSFFA
                     ALILPHAWLEIPAALLATAAVLRLGALLATPAQGKKIGQVWLESVAVWAQIMIGIVIP
                     ALFIAAMIEVWITPHLASLILR"
     gene            1800..>2185
                     /locus_tag="PKL11_11420"
     CDS             1800..>2185
                     /locus_tag="PKL11_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ14253.1"
                     /translation="MGRLIVLGTANAVANEHHENTHFVLLVGERAIMVDCVGNPVIRL
                     RQVGIDFNQVTDLILTHFHPDHVSGMPLLLMDMWLMQRKRPLHIYGLKHTIDRAITTM
                     TLYEWETWPNFFPVIFHRLPDEEKAT"
BASE COUNT          401 a          663 c          551 g          570 t
ORIGIN      
        1 tcaccgaagc cgaagatctg gcagatcaaa ttgccatcat ccagcgcggg cgcattatct
       61 ttaacgatac atccgccaat ctcaaacggc gcctgctggg cgctcccgaa tacgaggccc
      121 gcctggctgg catagtcaat ggttgggaac ccaccaatat tccgggcgtt tccatcgttc
      181 agcgcacaag cgatgtcctc agatttcggg ttgaagaccc ccaatcagcc aacccactgc
      241 ttctcgagca aatgatcgcc catcgcctgc ccgttgtctc tttccatgag gttcaccgca
      301 gcctcgaaga tgcctacctt gaggccgttc gtcaggccgc cgccgaggtg ccccatgctt
      361 aaatccctct atccggtcta tgttattgcc caccgcgaaa tccgtgacca gttccgtgac
      421 tggcgcattg tcttccccat catcatcctc accatcctct tcccattcct gatgaattac
      481 actgcccagg aaatgctggg ctttgtcggc aagtacggcg gtgaaattgt cggggagcgt
      541 ttgatcccct tcctgctgat gattgtgggc tttttcccca gctccttctc gctgattatt
      601 gccctggaaa cctttgttgg tgaaaaagaa cgcggctcca tcgaaccgct gctcagcacc
      661 ccgctggaag actggcagtt gtatctcggc aagctttcct cggcggtggt tcctccgctc
      721 tttggcagct tcctcggcat gacggtctat ctgactggac tggcaattgg ccgcgccccc
      781 ttgcccgatg gcgaaatgat gaccctcatc attttattga ccatcgtcca ggctgtcatg
      841 atggtttcgg gcgccgtggt tgtttcctgt caggccacct ctgtgcgggc tgccaacctg
      901 ttgtccagct tcattattat ccccgttacc ctgctgatcc agggcgaatc catgttgatg
      961 ttctggggca attatggcac cctgtggatg gccttctttg gcgtcatttt gctcaccttc
     1021 ctgctgatgc gcatgggcct ggcttacttc cgccgtgaag agctgctggg ccgtgaaatt
     1081 gatgtcctca atctgcgctg gggttggatg actttctggc aggccttcac gggcggcgcc
     1141 aaaaacgtct ggcagtggta tcgccgggtc atcccaaaaa cgctggccgc tttgcgcctg
     1201 ccgtttgtga tcgtctttct catgggcgct gcgggcgttg ccattggttt ctccctggtg
     1261 cgccagttcc ccattaccgc cccgcctgaa atgttcacca atctcgaaga acgcctcggc
     1321 ctgcttactg aagccttcaa gcccggcact ttcggcccgg tactgatggt ttttggcaac
     1381 aacttgcggg tcatggcgct ggctttgctg ctgggcattt tctcattcgc agtcttgggt
     1441 gtcttgccca ttattgtcac gctggctgtg gtcggctaca ttctgggcat ccttggattg
     1501 aacggcctgc ccgcaggctc tttcttcgcc gccctcattt tgccccacgc ctggctcgaa
     1561 atccctgctg cgctcctggc aaccgcagcc gtattgcgtt taggcgcctt actggcgacc
     1621 ccggcccagg gcaaaaagat cggccaggtc tggctcgaat cggtggcagt ctgggcgcaa
     1681 ataatgatcg gaatcgtgat tcccgccctg tttattgccg caatgatcga agtttggata
     1741 actccgcacc tcgcatcttt aattttgcgt taaaataaag agcgaggaga cgcgatccta
     1801 tggggcgctt gattgtttta ggcacagcta acgcagttgc caatgaacac cacgaaaaca
     1861 ctcacttcgt gctgctggtt ggcgaacgcg caattatggt ggactgcgtg ggcaaccccg
     1921 taatccgttt acgccaggtt ggcattgact tcaaccaggt gaccgatctc atcttaaccc
     1981 acttccaccc tgaccatgtt tcagggatgc cccttttatt gatggatatg tggctcatgc
     2041 agcgcaagcg cccgctgcat atctatggtc tcaaacatac catcgaccgc gccattacta
     2101 ccatgacgct ctacgagtgg gaaacctggc ccaacttctt ccccgtcatt tttcatcgcc
     2161 tgccggatga agaaaaagct accat
//
LOCUS       DAOPVS010000261         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_54209_length_2169_cov_56.140492, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000261 DAOPVS010000000
VERSION     DAOPVS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54209_length_2169_cov_56.140492"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKL11_11425"
     CDS             <1..576
                     /locus_tag="PKL11_11425"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNZ14254.1"
                     /translation="AFGISFGLMAFTTFVYDTLDICTRLGRYIIQELTGFKSWAGKIF
                     ATLLTGGVPLLLMLITLKDAAGNPVPAWKMFWTTFGASNQLLAGLALIGITVWLLNTA
                     KFKKAWIFTFVPALFMFIMSTWALIKMFLTYTVVEGVFAFPAGAQIIIPIACLIYIVL
                     AIWMAVETTLAFSRSKNNPSAPAEALGAPAK"
     gene            785..>2169
                     /locus_tag="PKL11_11430"
     CDS             785..>2169
                     /locus_tag="PKL11_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14255.1"
                     /translation="MKKRIQFTDLTLRDGNQSIAATRMTREQSLRVLKMIDEAGFQAM
                     ELWGGAILDSCVRFLNEDPWERLEAFTLMLGGPQKIQALLRGQNLFAYQPYADDLVIA
                     FVKQAVASGIGIMRMFDALNDERNLITALLATKAYGAKAEAAISYTTSPVHTIDYFVR
                     YAQKLEDMGADQLAIKDMAGLLHPKDAVALIRELRAHTTLPLALHSHTTTGVGLLNGV
                     IGMVYGVDSIDTAITPFAGGSSHPPVELLIVFAEALGLDHGLNKRLLLKIQAELFKIF
                     EELKNTIPYYGKFYRPVDFRDVNSRQVDDILRLVANPTTDNIESALKISHQMLADLGY
                     PSHDDRIFQSQIPGGMLSNLHNQLKQMGQPEMMDALMAEIPHVRRDVGYVPLVTPTSQ
                     IVGSQAAFNLISGERYALASDEFRMLLRGEFGRTPVPPNPEVVRRVLGDNESPLKYRP
                     ASYLPPVLEDT"
BASE COUNT          456 a          685 c          509 g          519 t
ORIGIN      
        1 gcctttggca tctccttcgg tttgatggcc ttcaccacct tcgtctatga caccctggat
       61 atctgcaccc gcctgggtcg ctacatcatc caggaactca ccggtttcaa gagctgggct
      121 ggtaagatct ttgccacgct cctcaccggc ggcgttcccc tgcttctgat gctcatcacc
      181 ctcaaggatg ctgctggcaa ccccgtgcct gcctggaaaa tgttctggac cacctttggc
      241 gcttccaacc agcttcttgc cggccttgcc ctcatcggca tcaccgtctg gctgctcaac
      301 acagccaaat ttaagaaagc ctggatcttc accttcgtcc cggcgctctt catgttcatt
      361 atgagcacct gggctctgat caagatgttc ctgacctata ctgtggttga aggcgtcttt
      421 gccttccccg cgggcgctca gatcatcatc cccatcgcct gcttgatcta catcgtcctg
      481 gcgatctgga tggctgtgga aaccaccctg gctttctctc gcagcaagaa taacccctct
      541 gccccagctg aagcccttgg cgctcctgcc aaataatcgc tgattacagt attgctcaat
      601 ataaccgcct gcccggtttt gggcaggcgg tttattatat taatcatgcg taagaatcgt
      661 gtaaaatcgc accctttcac ccggtagccc ggttataatg taaatagagc ttgaagtctc
      721 tctgcattaa ccgtcacagc gcccggcgct gtatctcact taacatgatt taacagggtg
      781 aaaaatgaaa aaacgaatcc aatttactga cctgacgctg cgcgacggca atcagtcgat
      841 tgccgctacc cgcatgacgc gtgaacaatc cctgcgcgtg ctcaaaatga ttgacgaagc
      901 tggttttcaa gccatggaac tctggggcgg cgcaattctg gatagctgtg ttcgcttcct
      961 gaacgaagac ccctgggaac gccttgaggc ctttaccctc atgctgggcg gcccccaaaa
     1021 aattcaggcc ctgctgcgcg gccaaaacct gtttgcctac cagccctatg cagatgacct
     1081 ggtcatcgcc ttcgtcaaac aagccgtggc ctcggggatt ggcatcatgc gcatgttcga
     1141 tgctctgaac gatgaacgca acctcatcac tgccttgctc gctaccaaag cctatggcgc
     1201 taaagccgaa gctgccatct cctacaccac cagtccggtt cacaccattg attacttcgt
     1261 ccggtatgct caaaagcttg aagacatggg cgccgatcaa ctggccatca aagatatggc
     1321 tggtttgctg caccccaaag acgccgttgc gctcattcgt gaactgcgcg cccacaccac
     1381 gctgcccctc gcactacatt ctcataccac caccggggtt ggccttctca atggtgtgat
     1441 tggcatggtc tatggcgttg actccatcga cactgccatc accccctttg ctggcggcag
     1501 ctctcaccca cccgtagagc tcttgattgt ctttgccgaa gccctgggcc tggatcacgg
     1561 cctgaacaag cgcctgctcc tcaaaatcca ggccgaactc tttaagattt ttgaggaatt
     1621 gaaaaacacc attccctatt acggcaagtt ctaccgcccg gtggatttcc gtgatgtgaa
     1681 cagccgccag gtggatgata ttctgcggct ggttgctaac cccaccaccg ataatatcga
     1741 atcggccctc aaaatctctc accagatgtt ggccgacctg ggctatccca gtcacgatga
     1801 ccgcatcttc cagtctcaaa tccccggcgg catgttgagc aatttgcaca accagctcaa
     1861 acagatgggg cagcctgaga tgatggatgc cctgatggct gagattcccc atgtgcggcg
     1921 cgatgtaggc tacgtcccgc tggtcacccc caccagccag attgtcggct cacaggccgc
     1981 cttcaatctg atttcgggtg agcgctatgc cctcgcctca gatgaattcc gcatgttact
     2041 gcgcggcgaa tttggccgca cccccgttcc ccccaatcca gaggtggttc gccgggtgtt
     2101 gggtgacaac gaatccccgc tgaagtaccg cccggcatca tacctgcctc ccgtgctgga
     2161 agacactta
//
LOCUS       DAOPVS010000262         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_54719_length_2156_cov_38.956211, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000262 DAOPVS010000000
VERSION     DAOPVS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54719_length_2156_cov_38.956211"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..631)
                     /gene="thpR"
                     /locus_tag="PKL11_11435"
     CDS             complement(47..631)
                     /gene="thpR"
                     /locus_tag="PKL11_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNZ14256.1"
                     /translation="MSEIRAFIAIELPEAIHKALEELSRKLQLEIPSGVRWVATENQH
                     LTLKFLGESAPGKLQLLSRSLPAALRTHAPVTFSVGGLGAFPSLRRPRVLWVGIQAGP
                     ELAELHKAVEMAAVGLGYAAEDKAFSPHLTLGRAQQHLPPRQIEQIADGLSRVSAQRL
                     GSLTADSVCLFKSDLRPEGPIYTPLARFKIGNAG"
     gene            732..908
                     /gene="ssrS"
                     /locus_tag="PKL11_11440"
     ncRNA           732..908
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKL11_11440"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1037..1699
                     /locus_tag="PKL11_11445"
     CDS             1037..1699
                     /locus_tag="PKL11_11445"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNZ14257.1"
                     /translation="MSSMFQLVVRAGNLTGQSFALDRPEIVLGRDLASDIVVTDAEVS
                     RRHARIFLKGTSYVLEDLGSTNGTFVNGQRLTGPYILQPGEYINLGENATIEFVAVEI
                     DPDATLISSNLRPAAAPLTPEPAYAPPPEQPAPLKPMPAPMPAPAAPQPAYVGQAPAQ
                     PSPVTNPPARKKMSWIVWLLIALGVLILGCILLLVIIDVANLWCTLGGSLFNSIWPGS
                     CP"
     gene            1715..>2156
                     /locus_tag="PKL11_11450"
     CDS             1715..>2156
                     /locus_tag="PKL11_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNZ14258.1"
                     /translation="MGKSKSEPQQNNPSPPSVNPAPQPDQNSSPLWGNNVKLIVGLTL
                     MAILAGLLIRFRNLIGPLLVAFILAFLLHPIAAWLQKRLNSKWRLSVNLVYLVLLILL
                     IGSLTLGGFALVEQVQNLISFVQRQIAAFPAWFDKITATPLIIGP"
BASE COUNT          468 a          650 c          496 g          542 t
ORIGIN      
        1 tcacctcata agaattgatt tgggtatatt ttagcataaa tctcaatcat ccggcattcc
       61 ctatcttaaa tctcgccagt ggagtgtaga ttggcccttc gggccttaaa tcgcttttga
      121 acaggcacac actgtcggct gtaaggctgc ccagacgctg tgccgaaacc cggctgaggc
      181 catccgcgat ttgctcaatt tgccggggtg gcaggtgctg ttgagcccgc cccagtgtca
      241 ggtgaggtga aaaagcctta tcttcagccg catatcccag ccctaccgct gccatttcta
      301 ccgctttgtg cagttctgcc agctcaggcc cggcctgaat acccacccac aacacccgcg
      361 ggcggcgcag actcggaaag gctcccaacc caccgacgga aaacgttacc ggtgcatgtg
      421 ttcgcaaagc cgctggcagc gaccgactta acaactgcag cttgcctggc gccgactcgc
      481 ccagaaattt gagcgtaagg tgttgatttt ccgttgccac ccagcgcaca ccgctcggaa
      541 tttcaagttg cagcttgcgg cttagttctt caagggcttt atggattgct tccggtagtt
      601 caatggcaat aaaagctcgg atttcactca tcaggtttcc ttatgatcaa atctacactt
      661 cttttcatta tagcaaactt gtcgccattc gtaatcctct acacatttgc ccgcgaatgt
      721 ggtataagaa aagtgccctg ctgtgttcgt gatgcagcat gaccggtttt gagccaacac
      781 tttttaattg ggaccctcta ttatatagat gacgcgatag gcctcggcca aggcgaagtc
      841 tatgtagcgg aacccagcct gcgcaagcag gttcaaaact ttgcagcaca cggcatggtg
      901 gggcgctttt ttcaagatca agatcaacgg ccataaccgt tgatttttgt ttccttccag
      961 cgattgacat tcatccacaa tgacattaaa ctaactttat agccatcttt aaaatttctc
     1021 attcaggagc aatccaatgt catcaatgtt tcaactggtg gtgcgtgccg gcaatttgac
     1081 cgggcagtct ttcgctctgg atcgcccaga aattgtttta ggacgagatt tagccagcga
     1141 cattgtcgtc acagacgccg aagtctcccg ccgccacgcc cgcatcttcc tcaaaggcac
     1201 cagttatgtg ctggaagacc tcggttccac caacggcacc tttgtcaatg gtcaacggct
     1261 gactggccca tatatcctgc agcctggcga gtatattaac ctgggcgaaa acgcaaccat
     1321 cgaatttgtc gctgtagaaa tcgatccaga cgccacatta atcagcagca acctgcgccc
     1381 cgctgcggcc ccgctcacac ccgaaccagc atatgcaccc ccgccagagc agcccgcacc
     1441 gctgaaaccc atgccagcgc caatgcctgc cccggccgcg cctcagcctg cctatgttgg
     1501 acaggccccc gcacagccat cccctgtgac caacccgccc gcccgtaaga aaatgtcctg
     1561 gattgtctgg ttgctgatcg cactgggtgt cttgatcttg ggctgcattc tcctgctggt
     1621 catcattgat gttgccaacc tctggtgcac cctgggcggc agcctcttca acagcatctg
     1681 gcccggctct tgcccgtaaa gtgggttata tcctgtggga aaatccaaat ctgaacccca
     1741 gcaaaataat ccatcaccgc cttctgtgaa tccggctcct caacccgatc agaacagttc
     1801 cccactctgg ggcaacaacg tcaagctgat agttggtctg acgttgatgg caatcctggc
     1861 gggcctgctc attcgattcc gcaatctcat tggcccgctc ctggttgcct ttatcctggc
     1921 cttccttctt caccctattg ctgcctggct gcaaaaacgc ctcaattcca agtggcggct
     1981 ctctgtcaat ttggtttacc tggtcttact catcttgctg attgggtcat tgaccttggg
     2041 cggttttgcg ctcgtagagc aggtccaaaa cctgatcagt ttcgtccagc gtcagatcgc
     2101 cgccttcccc gcctggtttg ataaaatcac tgccacgccg ctcattattg gcccct
//
LOCUS       DAOPVS010000263         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_55136_length_2145_cov_57.054545, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000263 DAOPVS010000000
VERSION     DAOPVS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55136_length_2145_cov_57.054545"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /gene="aspS"
                     /locus_tag="PKL11_11455"
     CDS             complement(<1..1316)
                     /gene="aspS"
                     /locus_tag="PKL11_11455"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNZ14259.1"
                     /translation="MERSMIGSLRQAIGQKVTISGFLQTLRDQKKMQFLIIRDTTGVV
                     QVFHWKANDPALAEFISTLTPETAVTITGTVADNPSVKLNGMEVQLESLRLENLAETP
                     LPLDLFAEALPPVDYRMDWRYLDLRRPASQLIFQVQTTAEMAMREYWIQHNFMEIHTP
                     KLLSAPSESGAELFEVDYFERKAYLAQSPQFYKQMAMAAGFDRVFEIGPVFRADPSFT
                     ARHMTEFTGVDMEISWIESHEDVMAFMEGWLQYVYQRVADAHGQAIKEVLGYELVVPT
                     LPFPRIPMAEAIEILKARGYELPADKKGDIDPTGERMLGQYVKETYNHDFVFVTDWPI
                     SVRPFYHMRYADNPNLTMSFDLLACGLEITTGAQREHRVEVLKAQALEKGLTLEGIQF
                     YLDFFRYGCPPHGGFGLGLSRLLMVMLGIQNIRETVYLFRGPTRLTP"
     gene            complement(1406..2059)
                     /locus_tag="PKL11_11460"
     CDS             complement(1406..2059)
                     /locus_tag="PKL11_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14260.1"
                     /translation="MKNKGLWTGLIILTLIFAAFWCALGVASLHLLPAPPDVSALPIL
                     DPEPTYTPDDLFVMLIRLEDLPAEPKQVTGPYYDDLGYKRNYIDHVNVQFTGGLVPND
                     WTYSHNIYQFGSELTAQDDYASALSRLVGFSAPAAEALPPLRADEFNLVCTEIASRAS
                     PYCIWTARYDTLVVELMAWLEPEHLSFEDLQAMIGVLDERISGIIQDGGTRPWGQDF"
BASE COUNT          442 a          585 c          686 g          432 t
ORIGIN      
        1 ggcgtcaggc gggtggggcc gcggaagagg tacacggttt cgcggatgtt ctggatgccc
       61 agcatcacca tcagcaggcg ggagaggccc agcccaaagc cgccgtgcgg cgggcagcca
      121 tagcggaaga aatcgaggta gaactgaatg ccttccagcg tcaggccttt ttcgagcgcc
      181 tgggccttca atacctccac gcggtgctcg cgctgtgcgc cggtggtgat ctccaggccg
      241 caggccagca ggtcgaagct catggtcagg ttggggttgt cggcatagcg catatgatag
      301 aacgggcgca cgctgatcgg ccaatcggtg acgaagacaa agtcgtgatt gtaggtttct
      361 ttcacatact ggcccagcat ccgctcgccg gtggggtcaa tatcgccctt tttgtcggca
      421 ggcagctcgt agccgcgcgc tttgaggatt tcgatggcct cagccatggg gatgcgcggg
      481 aagggcaggg tgggcaccac cagctcgtag cccagcacct ctttgatcgc ctgtccgtgg
      541 gcatcggcca cgcgctgata gacgtactgc agccagcctt ccataaaggc catcacatct
      601 tcgtgcgatt cgatccacga gatttccata tccacgccgg tgaactcggt catgtggcgg
      661 gcggtgaaag acgggtcggc gcggaagacc gggccaattt caaacacacg gtcaaaacca
      721 gcggccatgg ccatctgctt gtagaactgc ggcgactgcg ccaggtaggc cttgcgctca
      781 aaataatcca cctcaaacag ctcggcgccg ctttcgctgg gcgcgctgag cagcttgggg
      841 gtgtgaattt ccataaaatt gtgctggatc cagtattcgc gcatcgccat ttcggcggtg
      901 gtctgcacct ggaagatcaa ctggctggcg gggcggcgca gatccaggta gcgccagtcc
      961 atgcggtaat cgacgggggg cagggcctcg gcaaacaggt cgaggggcag cggggtttcg
     1021 gccaggttct ccagccgcag cgattcgagc tgcacttcca tgccgttcag tttgacggag
     1081 gggttatcgg ccacggtgcc ggtaatggtg acggcggttt cgggcgtcag cgttgagata
     1141 aactcggcca gggcggggtc gttggccttc cagtggaaga cctgcaccac gcccgtggta
     1201 tcacggatga ttaaaaattg catcttcttc tggtcgcgca gggtttgcag gaaaccagaa
     1261 atcgtcactt tctgaccaat ggcctgtcgc aggctgccaa tcattgagcg ttccatagat
     1321 aaatctctcc aatcgaatta acaattggaa taattatagc cccaatcgcg ggaattttta
     1381 ctttgaaatt gccgattcgc gaaagctaaa aatcctggcc ccaggggcgg gtgccgccat
     1441 cctggatgat gccggagatg cgttcatcca acacgccgat catggcctgc agatcctcaa
     1501 aagagaggtg ctccggctcc agccaggcca tcagctcgac caccagcgta tcgtagcgcg
     1561 ccgtccagat gcagtagggg ctggcgcggc tggcaatctc ggtgcagacc aggttgaact
     1621 catcggcgcg cagcgggggc agggcctcgg ctgcgggcgc cgaaaagccc accaaccggc
     1681 tcagcgcgct ggcataatcg tcttgagctg tgagttctga gccaaactgg tagatgttgt
     1741 ggctgtaggt ccaatcgttg ggcaccaggc cgccggtgaa ctggacattg acatggtcaa
     1801 tgtaattgcg cttgtagccc aggtcatcat aatacggccc ggtgacttgt ttaggctcgg
     1861 cgggcaggtc ttccagccgg atgagcatga cgaacaggtc atcgggagtg taggttggct
     1921 cggggtccag gatgggcagg gcgctgacat ctggcggggc gggcagcagg tgcaggctcg
     1981 ccacccccag ggcgcaccaa aacgcggcga agatgagggt caaaatgatc aggccagtcc
     2041 agagaccttt gtttttcatt ggcttctcca atgtgagacg ttgaggttgc agatgccacc
     2101 catgggttaa ttatacccgg ataataaaat caagcagggc ggggg
//
LOCUS       DAOPVS010000264         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_55595_length_2133_cov_58.183349, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000264 DAOPVS010000000
VERSION     DAOPVS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55595_length_2133_cov_58.183349"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKL11_11465"
     CDS             complement(<1..605)
                     /locus_tag="PKL11_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14261.1"
                     /translation="MKRTLSTLFSFLLLAGLVFGLLQPVSAMGTTPQATDKIETAVLQ
                     KIEATGSSDFVIVMAEQADLSAAYAITDWVARGDYVVETLKATAERTQKSVIAMLEKE
                     GLNYQSFFSGNEVQVYGGGNRVVLALAALPEVSHIRYPRTVHINPTVNLSPVTVEAKD
                     EINALDWGITDTNADEFWTTFGNQGTGILVANIDTGVQWDHS"
     gene            839..>2133
                     /locus_tag="PKL11_11470"
     CDS             839..>2133
                     /locus_tag="PKL11_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14262.1"
                     /translation="MTDEKQSSIFCLDGCPAGLRHLLPGAAANSATPTPLQTGACIPN
                     ALALTTGDLETLRAEILAQGGCVVHLFPPHALMGRLPRRVPGATIIRGEANVDRIQAS
                     HGAEAALAAAVWNANVIHPQPQAAPSDTALPLTGDVIRLPEVQALINAQMPQAAPGYY
                     DASEFMAGRIVVGIILPESNGVQEPSTEDWDLVRISQVVTEIQAGMDWWNTTNPQGNL
                     EFVYDIHSQVPSVYEPINWGWPSDNLWISDTLGRLGYASGDWVERSYAYLNAVRTENN
                     ADWAVVIFVADSLKDTDGIFNNGQYFGYTYGDPPLVVMTYDNDGWGIGNMNSVTAHEF
                     GHDFGAGDEYCSPGYACCSCSERLGYLGVYNTNCEAGCYSTPNGCTTCVQITCLMRQG
                     NVSSGLCEISRGQVGMRDTDGDGRWDPVDTFAQFTLTAQP"
BASE COUNT          413 a          636 c          638 g          446 t
ORIGIN      
        1 gagtggtccc actgcacgcc ggtatcaata ttggcgacca gaatgcccgt gccctggttg
       61 ccaaaggtgg tccagaactc atcggcattg gtgtcggtaa tcccccagtc cagggcgttg
      121 atttcgtcct tcgcttcgac ggtgaccggg gagaggttga cggtggggtt gatgtggacc
      181 gtgcgcggat agcggatgtg gctcacttcc ggcagggctg ccagcgccag taccacgcgg
      241 ttgccgccgc cgtacacttg cacctcgttg ccagagaaga aggattgata gttcaggcct
      301 tctttttcca gcatggcgat gaccgatttc tgggtgcgtt cggcggtggc tttgagggtc
      361 tccaccacat agtcgccgcg cgccacccag tcggtgatgg cgtaagcagc gctcaggtcg
      421 gcctgttcgg ccattacaat cacaaaatct gagctgccag tagcttcgat tttttgcagt
      481 acggcagttt cgattttgtc ggttgcctga ggggtcgttc ccattgcgct gacaggctga
      541 agcaggccaa agaccagccc ggccagcagc aggaacgaaa acagggttga gagagtgcgc
      601 ttcatacgat ctccttagat atgggacaat gaaacaaggg ggaagaatca gcgtacgccc
      661 gtacgctaca gaccattgta aaattaaatc cagattaata caagagggtt tataattatg
      721 ccgtttggca agctgcgcgg ccctttacag ggcgctgcgc ggtcctttac tggcaccccc
      781 attgttttca attgcctgct tttgcccggc aatttagatt tcccccattc attcgaggtt
      841 gactgatgaa aagcaaagct ctattttttg tcttgatggc tgccctgctg ggctgcgcca
      901 tcttcttccc ggcgcggcgg ccaactcggc cacccccacg ccgctgcaaa ccggcgcctg
      961 catccccaat gccctggcgc tgaccaccgg cgacctggag accctgcgcg ccgagatcct
     1021 ggcccagggc ggctgcgtgg tgcatctctt cccgccgcac gccctgatgg gccgcctgcc
     1081 gcggcgtgtg cccggcgcca ccatcatccg cggcgaggcc aacgtggacc gcattcaggc
     1141 cagccacggc gctgaggccg cccttgcagc tgccgtctgg aatgccaatg tgatccaccc
     1201 gcagccccag gctgccccca gcgacaccgc cctgccgctg acgggggatg tcatccggct
     1261 gccagaggtg caggccctga tcaatgcaca aatgccgcag gccgccccgg gctattatga
     1321 tgccagtgaa tttatggccg ggcgcattgt ggtgggcatc atcctgcctg aaagcaacgg
     1381 cgtgcaggag ccttccaccg aagactggga cctggtgcgc atcagccagg tggtgaccga
     1441 aattcaggcc ggcatggact ggtggaatac caccaacccg cagggcaacc tggaatttgt
     1501 gtatgacatt cacagtcagg tgcccagtgt ttacgaacca attaactggg gctggcccag
     1561 cgacaacctg tggatcagcg atacgctggg acggctgggc tatgccagcg gtgattgggt
     1621 ggaacgttcc tacgcctatc tgaacgccgt gcgcacagaa aacaacgccg actgggctgt
     1681 ggtgattttt gtggccgaca gcctcaaaga taccgatggc attttcaata atggtcagta
     1741 ctttggctat acttacggcg acccgccgct ggtggtgatg acctatgaca acgacggctg
     1801 gggcattggc aatatgaaca gcgtcaccgc ccacgaattt gggcacgatt ttggcgccgg
     1861 agacgagtac tgctcgccgg ggtatgcctg ctgctcctgc tccgagcgcc tgggctatct
     1921 gggcgtttat aacaccaact gcgaggccgg gtgctattca acccccaacg gctgcaccac
     1981 ctgtgtccag atcacctgcc tgatgcgcca gggcaatgtc agctcgggcc tctgtgaaat
     2041 cagccggggg caggtgggca tgcgggatac tgatggcgat gggcgctggg acccggtgga
     2101 tacctttgcc cagttcaccc tcaccgccca gcc
//
LOCUS       DAOPVS010000265         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_56388_length_2112_cov_56.749635, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000265 DAOPVS010000000
VERSION     DAOPVS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56388_length_2112_cov_56.749635"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1275
                     /locus_tag="PKL11_11475"
     CDS             133..1275
                     /locus_tag="PKL11_11475"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ14263.1"
                     /translation="MINRPFPIDQIHQALRRSPITALLGPRQCGKTTLARVVAQGQPA
                     TFFDLESVPDQRRLQNPNLVLADLTGLVVLDEIQSMPEIFNVLRVRVDRPDNQARYLI
                     LGSASPLVVKHISESLAGRVEFVELSGLRLDEVGISDWQSLWLSGGFPRSYLASNETD
                     SLAWREGFIRTFLERDIPQLGINIPAAAMRRFWMMLAHYHGQTWNASELGRSLGINDK
                     TVRYYLDILTGTYMVRQLQPWYENLNKRQVKAPKVYFRDSGLLHALLGISDWNGLLGH
                     PKVGASWEGFVIEQVLQLIQPPTAYYWGVHGSAELDLFYILNGKRYGIEVKFSEAPEI
                     TASMHVALQDLKLDHLWVIHPGSAGYPVHERITVLPISQLAKLQSV"
     gene            1288..2049
                     /locus_tag="PKL11_11480"
     CDS             1288..2049
                     /locus_tag="PKL11_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNZ14264.1"
                     /translation="MPPFPILDFDPDRNAIINPPPAKPGEELPARVVPCFFQDVLRDL
                     KAAGRLTQIYELRSELGPVPVYRLEGDHPAVTVYHAGLGAPLAAGLLEEIIGLGGRYF
                     IACGGCGVLDGAHDVGHLFLVTHAVRDEGMSYHYLPPEAPALASPRAVAALEATLKRH
                     DLPIHPTKTWTTDAFYRETPARRDRRVAEGCEVVEMEAAAFFAVAAFRGVEFGQLLYG
                     GDVVIPDGWDRRGWHTRGDIRLNLFELAAEAVLQI"
BASE COUNT          421 a          595 c          615 g          481 t
ORIGIN      
        1 tgatggcaca gcgctttgcc gccgtgcccg gcatcgaatg gcgcggctgg ccggcagatt
       61 agggcgggtc aaaagagcag tactcatgaa atctcggatt gtaattccga aatttcatga
      121 taaaattaaa gcatgattaa tcgccccttt cccattgacc aaattcatca agccttgcgc
      181 cgttcaccaa ttacagcgtt gttggggccg cgtcagtgcg gcaaaacaac gctagctcgg
      241 gtagtggcac aggggcaacc cgccaccttc ttcgacctcg aatcggtacc ggatcagcgg
      301 cggctgcaaa accccaacct ggtgctggct gacctgaccg ggctggtggt actggatgaa
      361 attcaatcca tgccggagat attcaatgtg ttgcgcgtgc gggttgatcg accagacaat
      421 caggcgcgct acttgatcct gggcagtgct tctccactgg tggttaaaca tatttccgaa
      481 tctttggccg gccgggtaga gtttgttgag ctttcaggtc tgaggctgga tgaggtcggt
      541 atatcagact ggcagtcgtt gtggctgagc ggcggctttc cacgcagtta tctggcttca
      601 aatgaaaccg acagcctggc ctggcgtgag ggcttcattc gaaccttcct ggaaagggac
      661 atacctcaac tagggatcaa cattccggcg gcagccatgc gccggttctg gatgatgctg
      721 gcccactacc atggtcaaac ctggaatgcc tctgaattgg gacgttcact gggcattaac
      781 gacaaaacgg tgcgctatta cctggatatt ctaacgggta cctatatggt tcggcagtta
      841 cagccctggt atgaaaactt gaacaagcgt caggttaagg cacccaaagt ctattttcgt
      901 gactcggggc ttttacatgc cctgcttgga atttcagatt ggaatggctt gttggggcat
      961 cccaaagtgg gggcctcatg ggaaggcttt gtgattgaac aggtcttaca gcttatccaa
     1021 ccgccaaccg cctactactg gggggtacac ggcagcgccg agttggattt gttctacatc
     1081 ttaaacggca aacgctatgg gattgaggtg aaattttctg aggcgccaga aatcacggct
     1141 tcaatgcatg ttgccctgca agaccttaag ttagatcatt tatgggtcat tcatccaggc
     1201 agcgctggct acccggtaca tgaacgcatt acggttttgc ccatctcaca gttagcaaag
     1261 ttacaatccg tctaggagcc catccccatg ccccccttcc ccatcctgga ttttgacccc
     1321 gaccgcaatg ccatcatcaa cccgcctccc gccaaacccg gcgaggaact gcccgcgcgg
     1381 gtggtgccgt gcttcttcca ggatgtgctg cgagatttga aagccgccgg ccgcctgacc
     1441 cagatctacg agctgcgctc agagctgggc ccggtgccgg tttaccggct ggaaggcgac
     1501 catcccgcgg tgacggtcta tcacgcgggg ctgggcgcgc cgctggctgc cggcctgctg
     1561 gaagagatca tcggcctggg cgggcggtat tttatcgcct gcggcggctg cggcgtgttg
     1621 gatggcgctc acgatgtggg gcatcttttt ctggtgaccc atgccgtgcg cgacgagggc
     1681 atgtcttacc attatctgcc gcccgaagcc ccggcgcttg ccagcccgcg cgccgttgcc
     1741 gcgctggaag ctaccctcaa gcgccatgac ctgcccatcc atcccaccaa aacctggacg
     1801 accgatgcat tctaccggga gacgcccgcc cgccgggacc ggcgcgtggc agagggctgt
     1861 gaggtggtcg aaatggaagc cgctgccttt tttgccgtgg cagccttccg gggcgtggaa
     1921 tttgggcagc tgctctacgg cggcgatgtg gtcatccccg atggttggga ccggcgcggc
     1981 tggcataccc gcggcgatat tcgactcaac ctgtttgaac tggcggcaga agcggtttta
     2041 cagatttaat atcaccccat tccggttaaa cacggaaggc cgtccttgcg ggcggccttc
     2101 gtttttgggc tg
//
LOCUS       DAOPVS010000266         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_57118_length_2093_cov_38.161923, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000266 DAOPVS010000000
VERSION     DAOPVS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57118_length_2093_cov_38.161923"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKL11_11485"
     CDS             <1..276
                     /locus_tag="PKL11_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14265.1"
                     /translation="LIIRAGWEWGMRLHNYLLVSAFLLIALIALLGLPWSLAWRAFLA
                     FPIAIYQIWQIWSISQGAPPNWKILRLTAVATVAMILYQWIIFLWTL"
     gene            292..1527
                     /locus_tag="PKL11_11490"
     CDS             292..1527
                     /locus_tag="PKL11_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNZ14266.1"
                     /translation="MPEFLTLVPPAEALNRLLDNLPLGPIGVETLPVPAALDRVVAQT
                     ILAVEPLPAFSRSTVDGYAVLAPDTHGASETLPAYLKILGEVSMGITPQFRIARGGAA
                     IIHTGGMLPPGADAVVMLEYTQQARPGEIEILRPVASGENLLLPGEDVQPGQVLIQAG
                     HSLRPAEMGGLMAQGLTTVVVSRRPRVAIISSGDELISPDRTPAPGQVRDINSYSLAA
                     LVQKHGGEPVLCGIVPDRPEALAEELRRVLPEADLTLVTAGSSASTRDFTARVIQQIG
                     SPGVLVHGVNVKPGKPTILAVCDGKPIIGLPGNPVSALVIAELFVAPVIHRLLGRIPP
                     QVGSIQARLTANLPSQAGREDYFPVRLTAQSGTLQAEPIFFKSNLIFSLSSADGLVCI
                     PADTTGLAAGEWVDVRMML"
     gene            1541..>2093
                     /locus_tag="PKL11_11495"
     CDS             1541..>2093
                     /locus_tag="PKL11_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNZ14267.1"
                     /translation="MSVYLTDIPLAEAQSCLLAALKAAGLDRILGIETIPLDEQALGR
                     VLAEPAWARMSSPHYHASAMDGFALRAEDTRGAQPSAPVTLAVNEQTIYVDTGDPLPD
                     WANAVIPIELVESLDDLTQIAADVRQPHNIRIRAAVTPWSHVRPMGEDIVATQLVLPA
                     GQVLRPVDLGALAAGGFTELRVSR"
BASE COUNT          382 a          644 c          561 g          506 t
ORIGIN      
        1 ttgatcattc gcgccggctg ggagtggggg atgcgccttc ataactacct gctggtttcg
       61 gcctttttgc tgattgccct gatcgcgttg cttggtctgc cgtggtcatt ggcctggcgg
      121 gcgttcctgg cgtttcccat tgcgatctat cagatctggc aaatttggtc aatctcccag
      181 ggcgctccgc ccaactggaa aatacttcgt ctgacggctg tggcaacagt ggcaatgatt
      241 ttatatcagt ggattatctt cttgtggacg ctctaataca ggattaaccc gatgcccgaa
      301 tttttgaccc ttgtccctcc cgccgaagcg ctgaatcgcc tgttggataa tctccctctg
      361 ggacctattg gagtggaaac acttcccgtt cctgctgcgc tggaccgcgt agtcgcccaa
      421 accatccttg ccgttgagcc cttgcctgcc ttttcgcgca gtacggttga tggttacgca
      481 gttctggccc cagacaccca tggcgccagc gaaacactcc ccgcttactt aaagattctg
      541 ggggaggtca gcatggggat taccccgcaa tttcgtatag ccagaggggg cgcggccatt
      601 atccatacag ggggcatgct tccccccggc gcagacgccg tcgtgatgct ggaatacact
      661 caacaggctc gtcccggtga aattgaaatc ctgcggcctg tcgcttctgg cgagaatctc
      721 ctgctgccgg gggaggatgt tcaacccgga caggttttaa tccaagccgg tcattccctc
      781 cgcccggctg aaatgggcgg gttgatggct cagggcctga ccaccgtggt tgtctctagg
      841 cgcccacggg tcgccattat ttccagcgga gacgaactga tttctcctga tcgcacgccc
      901 gctcccggtc aggtgcgcga tattaacagc tattctctcg ctgcgctggt gcaaaagcat
      961 ggcggcgagc cggttttatg cggcattgtc cccgaccgtc ctgaagcact ggctgaagaa
     1021 cttcgccgcg tcctccccga agctgacctg acattggtaa ccgccggttc atcggccagc
     1081 acgcgtgatt ttacagcccg cgtcatacag caaattggat cacccggcgt cctggtgcat
     1141 ggtgtcaatg tcaaaccggg caaacccacc atcctcgctg tctgtgatgg caaacccatt
     1201 attggcctgc ccggcaaccc ggtcagcgcg ttggtcattg ccgagctttt cgtcgccccg
     1261 gttattcacc gtctattggg gcgcatcccc cctcaggttg gatccattca ggctcgcctg
     1321 actgccaatc tcccatcgca agccgggcgt gaagactatt tcccggttcg cctgacggcc
     1381 cagtctggca cgcttcaagc tgaacccatc ttcttcaaga gcaatctgat attttcactc
     1441 tctagtgctg atggcctggt ctgtattcct gccgatacca ctggcctcgc cgctggcgaa
     1501 tgggtggatg tgcggatgat gctctaacaa aggatgccct atgtcagttt atttgaccga
     1561 tattcccctt gctgaagcgc aatcttgcct tctcgccgcc ctcaaagctg ctggactgga
     1621 ccgcatcctg ggtattgaga ccatcccgct cgatgagcag gctctgggcc gcgtcctggc
     1681 tgagccagcc tgggcacgca tgtcctcacc ccattaccac gcctctgcaa tggatggttt
     1741 tgcacttcga gcggaagata cccgtggagc acagccatcg gcaccggtaa cgctggcagt
     1801 caatgaacaa actatctatg tcgatactgg cgatccgctc cccgattggg ccaatgccgt
     1861 gatcccaatt gaactggtcg aatcattaga tgatcttaca cagatagctg cagacgttcg
     1921 ccagccgcat aacattcgta ttcgcgctgc cgtaaccccc tggagtcatg ttcgcccaat
     1981 gggcgaggac attgtcgcca cgcaattggt gctgccggcc gggcaggtcc tccgcccagt
     2041 ggacctgggc gctcttgctg ccggtggttt tactgaactc cgcgtcagcc gcc
//
LOCUS       DAOPVS010000267         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_57709_length_2078_cov_49.258527, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000267 DAOPVS010000000
VERSION     DAOPVS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57709_length_2078_cov_49.258527"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKL11_11500"
     CDS             complement(<1..623)
                     /locus_tag="PKL11_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ14268.1"
                     /translation="MRSINLNRTISYFKSLKGEYPRQFWLLFWGMLISATGSSMIWPF
                     LMIYVGDRLNLPLSSITFLTAVNALAGLSSFFVGPLVDRLGRKWVMAISLAMNGIGYI
                     LMSQAHTLGQFAALMALQGAFNPLYRMGSDAMIADLIPPEKRTDAYGLLRMVHNAGIA
                     LGPSMGGLIASQSYTLAFILAAASLLIYSAIVTFFCAETLVPHPDQAA"
     gene            741..962
                     /locus_tag="PKL11_11505"
     CDS             741..962
                     /locus_tag="PKL11_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNZ14269.1"
                     /translation="MLPEDLLAILRCPACVKETGGQLTLVKDSWLVCQDCGRKYPIVD
                     DIPVMLIDQGDRWRETSVDALPVPPPKMD"
     gene            973..>2078
                     /locus_tag="PKL11_11510"
     CDS             973..>2078
                     /locus_tag="PKL11_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ14270.1"
                     /translation="MGRENLQMARKKRVSLDRTSLIVLVVFIVLAIITAIVTFNFVRD
                     LVTGWSITPIDGVVINNPTAQPGGTVTGPTVPAPQGALQPVDSGPEALDWDGNTRVNI
                     LLMGLDYRDWEAGEYPRSDTMLLATVDPVTKTAGLLSIPRDLWVNIPGYDYAKINTAY
                     YLGEANKLPGGGPGLAIQTVEDFLGVPIHFYAQVDFTAFVRLVDEIGGVKIKVEEELT
                     IDPVGDSPKVHLTPGTYTLNGELALAYARNRKTDGGDFDRARRTQQVIMAIRDRVLQF
                     NMLPTLVAKAPILYEEVKAGVRTNMTLDQAIRLAALLQSLPAESIHNAVIGPGQVEPA
                     KSPDGLDILIPYPDQIRLVRDEIFSETGGPAAPVV"
BASE COUNT          491 a          525 c          635 g          427 t
ORIGIN      
        1 gcggcttgat cggggtgcgg caccagggtt tcagcgcaga aaaaggtaac aatggcgctg
       61 tatatgagca ggctggctgc agccaaaatg aaggccagcg tataggattg ggaagcaatc
      121 agaccgccca tggagggacc cagtgcaatg cctgcgttat gaaccatgcg cagcaggcca
      181 taggcatcgg tgcgtttttc gggcgggatc agatcagcga tcatggcgtc tgagcccatg
      241 cggtagaggg gattgaaggc gccctgcagc gccatcagag cggcgaattg acccagggta
      301 tgggcctgac tcatcagaat atagccgatg ccattcatcg ccaggctgat ggccatgacc
      361 catttgcggc caagccgatc taccagaggg ccgacaaaga aagatgacaa accagccaat
      421 gcgttgaccg ccgtcagaaa tgtgatggaa gacagcggca ggttcaggcg gtctccgacg
      481 tagatcatca gaaatggcca gatcatgctg ctgccggtgg cgctgatcaa catgccccag
      541 aagaggagcc agaactggcg gggatattcc cccttcaaag acttgaaata agaaattgta
      601 cgattaaggt ttatggagcg catggtttga cgataagcta acgaacctgg gatattggca
      661 cggatattgt accatgggga agtatcccgg ctcgtggtac aatagggaag agttttttaa
      721 tccaccaata tggaggcacc atgctccctg aagatttatt agccattctg cgctgcccgg
      781 cctgtgtgaa agaaaccggt ggtcaattga ccctggttaa agacagctgg ctggtatgtc
      841 aggattgcgg gcgtaaatac ccgattgttg acgatattcc tgtcatgttg attgaccagg
      901 gagaccgctg gcgcgaaaca agcgttgatg cgctgccagt tcccccaccc aagatggatt
      961 aatcaggccc atatgggacg cgaaaatctt caaatggccc gcaaaaagcg ggtaagcctg
     1021 gaccgcacca gcttgattgt gctggttgtc tttattgttc tggccatcat cacggcgatt
     1081 gtgacattca attttgtgcg cgatctggtg acgggctgga gtattacgcc cattgatggc
     1141 gtggtgatca ataaccccac agcacagccc ggcggcaccg ttacgggccc aactgtgcca
     1201 gccccgcaag gcgctctgca gccggttgat tctggcccgg aagcgctgga ctgggacggc
     1261 aatacgcggg tgaatatcct gctgatgggg ttggattatc gcgattggga agcaggcgaa
     1321 tatccgcgct cagacaccat gctgctggcg acagttgatc cggtgacaaa aacagccgga
     1381 ctgctgtcta ttccgcggga tttgtgggtg aatattcccg ggtatgacta tgccaaaatc
     1441 aacacggcat attacctggg cgaggcgaat aaactgcccg gcggtggtcc gggactggcc
     1501 attcaaaccg tggaagattt cctgggggtg ccgattcact tttacgcgca ggtggacttt
     1561 acggcctttg tgcggctggt ggatgaaatt ggcggcgtga agatcaaggt ggaagaagaa
     1621 ctgacaatcg acccggttgg ggattcaccc aaggttcacc tgacaccggg aacttatacc
     1681 ctgaacgggg agctggcgct ggcctatgcc cgcaaccgga agacggatgg cggcgacttt
     1741 gaccgcgccc gccgcaccca gcaggtgatt atggcgattc gtgatcgggt gctgcagttc
     1801 aatatgctgc cgacactggt tgccaaggca ccgattttgt acgaagaagt caaagcgggt
     1861 gtgcgcacaa acatgaccct cgaccaggca atccgcctgg cggcactgct gcaaagcctg
     1921 ccggctgaaa gcatccacaa cgcggtgatt gggcccgggc aggtggaacc ggcaaaatcg
     1981 cctgatggtc tggatatctt gatcccctac cccgatcaga ttcggctggt gcgggatgag
     2041 attttcagcg aaacaggcgg cccggcagcg ccggtggt
//
LOCUS       DAOPVS010000268         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_60090_length_2025_cov_32.662944, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000268 DAOPVS010000000
VERSION     DAOPVS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60090_length_2025_cov_32.662944"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKL11_11515"
     CDS             complement(<1..884)
                     /locus_tag="PKL11_11515"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HNZ14271.1"
                     /translation="MREKLCGTGQNFTKILLAQTGLIVRIFLKTKVLISGVYLSLHLR
                     VKMLPFVEKCLSTQLAELGRFAFPVSQIIMTALSAVDPRIATRRALESACPDFSQDPA
                     GRLLLLAFGKAAFPMTMGALDVLAGRFSDGVVVSKHIPSDAGALPASFRLLLGAHPVP
                     DERSQAAGLATLGFLSNLTDRDRVICLISGGGSALLTYPVDSLTLTELQQLTHQLLAC
                     GASITEINTLRKHLDRVKGGGLARAAAPARLDTFILSDVMGNPIDAIASGPTAPDPTT
                     YAEAWSILEKYHLESIAPA"
     gene            847..1404
                     /locus_tag="PKL11_11520"
     CDS             847..1404
                     /locus_tag="PKL11_11520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ14272.1"
                     /translation="MKFWPVPHSFSRTLPAEGDAGCFWQDRGDRRHAGVDIYAPAESD
                     VVAIEDGVVRSVELFTSPELRTYWNNTYAIVVQNSVGRVIRYAEMKSACVQPGDLVKA
                     GQKIGQVGQVLEPDRIDHTAPGYIQALKSNGVASMLHLEVHAGLPLDDEQYLGGNYFN
                     RPQPQGLINPMVVLDEFVDNHPQKG"
     gene            1488..>2025
                     /locus_tag="PKL11_11525"
     CDS             1488..>2025
                     /locus_tag="PKL11_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNZ14273.1"
                     /translation="MNVTNLDSLYRPKSIAVVGASNTPGKIGFTVVDNLLKSGYEGKI
                     FPINPKDPEVQGLKAYASVLAVPEAIDAAVIVVPASMAEQVTEDCGKKGVKALIVIAS
                     GFSEVGNRELEDRVVAIAKRYNMRILGPNIVGTLSNSDKMNASFAPFLPFAGKAALIS
                     QSGALLIAIDASTYIRGIG"
BASE COUNT          503 a          479 c          586 g          457 t
ORIGIN      
        1 gcgggggcaa ttgattccag gtgatatttt tccaaaatcg accaggcttc ggcgtaggtt
       61 gtggggtcgg gcgcggtggg accggaagca atggcatcga tggggttgcc catgacatcc
      121 gagaggatga aagtatccaa tcttgctgga gcagcggcgc gagccagacc tccgcctttg
      181 acacggtcga ggtgtttgcg taatgtgttg atttcggtga tggatgcgcc acaagcgagc
      241 aactgatggg ttagctgctg cagttcggta agggtaaggc tatcaaccgg ataggtgagc
      301 aaagcggaac cgccgccgga aatgagacaa atcacgcggt cccggtcagt gagattggat
      361 agaaaaccaa gagtggcgag acccgccgcc tgactgcgct catctggcac ggggtgggcg
      421 cccaggagta agcgaaaaga cgctggcaaa gcgccagcgt ctgatgggat atgcttggat
      481 acaaccacac catcagaaaa gcgaccggca aggacatcca gagcccccat ggtcattgga
      541 aaagcggctt ttccaaacgc cagtagcaac aatcgaccag cagggtcttg agagaaatct
      601 gggcaggcgc tttcaagcgc gcggcgggtt gcaatccggg gatcaactgc ggataaggcg
      661 gtcataatga tctgcgacac ggggaaagca aaccttccca gttcggcaag ttgagtggat
      721 aagcattttt caacaaaagg taacattttt accctcaaat gtaatgataa atatacaccc
      781 gaaatcagga cttttgtttt taaaaatatg cgtacaataa gaccggtctg cgcgaggaga
      841 attttagtga aattctggcc tgttccacat agcttttccc gcactttacc ggctgaaggc
      901 gatgccggtt gtttctggca agaccgggga gatcggcgcc acgcaggggt ggatatttat
      961 gcgcccgccg aaagcgatgt ggttgccatt gaagatggtg ttgtgcggag cgtggaactc
     1021 tttacatcgc ccgaactgcg cacgtactgg aacaatactt atgctattgt agtccagaat
     1081 tctgtcgggc gggttattcg atacgcagaa atgaaatctg cctgcgtgca acccggcgat
     1141 ttggtgaaag ccgggcagaa aattgggcag gttggacagg tgctggagcc ggatcgaatt
     1201 gatcacacgg cgccgggata catccaggcg cttaagtcta atggggttgc atccatgctc
     1261 catttggagg tgcatgccgg gctgccactg gatgatgagc agtatctggg cggcaattat
     1321 ttcaatcgtc cccaacccca gggcttaatc aacccgatgg ttgttttaga cgaattcgtt
     1381 gataaccacc cgcaaaaagg ctaatggcgg gttttgaggt atactatacc tttggtttaa
     1441 tctgataaaa gaaaaagttt ctttaatacc tttcaggagg atcaagcgtg aacgtaacaa
     1501 atctggatag tctctatcga cccaagtcaa ttgccgtggt gggcgcctcg aacaccccgg
     1561 ggaagattgg cttcaccgtg gtagataacc tgctcaagag cgggtacgag ggcaaaatct
     1621 tcccgattaa ccccaaagat cccgaagttc agggactgaa ggcctatgcc tctgttctgg
     1681 cagtgcccga agcaattgat gcggcagtga ttgttgtgcc cgcgagcatg gcagaacagg
     1741 ttactgaaga ctgcggtaaa aaaggcgtga aggctctgat tgtgattgcc tcgggcttct
     1801 ccgaagtggg caaccgcgaa ctggaagacc gggttgtggc gattgccaaa cgctacaata
     1861 tgcgcatcct gggcccgaac attgtgggca ccctgtctaa ctcagacaaa atgaatgcct
     1921 cgtttgcccc cttcctaccc tttgctggta aggccgccct gatttcgcaa agcggtgcgc
     1981 ttttgatcgc cattgatgcc tccacttata tccgcgggat tggtt
//
LOCUS       DAOPVS010000269         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_60424_length_2018_cov_46.996943, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000269 DAOPVS010000000
VERSION     DAOPVS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60424_length_2018_cov_46.996943"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKL11_11530"
     CDS             <1..212
                     /locus_tag="PKL11_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14274.1"
                     /translation="DVEQAMKPGYSTASEAVREMGFGAGMGLVNISRCTDSMTLESTV
                     GKGTRLRMKIFLAQQETVGEGYPAN"
     gene            224..568
                     /locus_tag="PKL11_11535"
     CDS             224..568
                     /locus_tag="PKL11_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14275.1"
                     /translation="MNLETIIKDLELSVLTERQDFSKITPSSGYTSDLLSCVMAGAPK
                     HSIWVTLQAHGNIIAVAALLDLSAIIVTEGAMPDPATLVKANEEGITILATPKPTFEI
                     VGRLWEMGLRAE"
     gene            589..1338
                     /locus_tag="PKL11_11540"
     CDS             589..1338
                     /locus_tag="PKL11_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14276.1"
                     /translation="MKLIRAELHVHTVLSPCAEIEMLPPLIVQEAVDREIQLIAITDH
                     NATGNIAAVQKAAEGLPLTVLPGMELQTREEVHNLCLFETLEETAVFQEWVNKHLPDL
                     LNRPDYFGEQLLVDENGDFVEKEPRLLLTSADVSIDEACAKVHEIGGIFIPAHVNRTA
                     NGLLPTLGFVPEGICLEALEISKHLKPDEARRKFPQIAGYPLIQSGDVHRLDDFIGVN
                     QFEIESPTLAEIKLALRGQEGRQYRILSRTI"
     gene            1496..2011
                     /locus_tag="PKL11_11545"
     CDS             1496..2011
                     /locus_tag="PKL11_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ14277.1"
                     /translation="MPNILDKIPANDPLNDPEKKRLIDEILEANKNLPGATMVALNEL
                     QSQIGFVSEPMQQYVAQKLRVPVSQVHGVISFYSFFTTQPRGKHTIKFCLGTACYVGG
                     VPQLMEKAKQLLGIEAGQTTPDGNITLEVCRCVGACSQAPVIVVDEHVHGRVKPNKFP
                     QLVRSVQEPEA"
BASE COUNT          514 a          496 c          544 g          464 t
ORIGIN      
        1 cggatgtgga gcaggcgatg aagccggggt attccacagc cagcgaagca gtgcgggaaa
       61 tgggctttgg ggccggcatg ggcctggtca atatttctcg ctgcaccgac agcatgacac
      121 tggaatcaac cgtgggcaaa ggcacccgcc tgcgcatgaa aatcttcctc gctcaacagg
      181 agactgttgg cgagggctac cccgccaatt aggagatgcc tgaatgaatc ttgaaacgat
      241 tatcaaagac ctggaattgt ctgtcttgac tgaacgccag gatttttcga aaattacgcc
      301 atcatcaggt tacacttcgg atttacttag ctgtgtgatg gccggagcgc ccaagcacag
      361 catctgggtg actctgcagg cgcatggcaa tattattgcc gtggcagcac tgctggattt
      421 gagcgctatc atcgtgaccg aaggcgcaat gcccgaccca gccacactgg ttaaagctaa
      481 cgaagaaggg atcactatcc tggctacccc aaaacccaca tttgaaattg ttggccggct
      541 gtgggagatg gggctgcggg ctgaataagc ccgaccctga caatgggctt gaagctgatt
      601 cgcgctgaac tgcacgttca taccgtgctg tcgccctgcg cagagattga aatgctgccg
      661 ccattgattg tgcaggaagc ggtagatcga gaaattcaac tgattgcgat tactgaccat
      721 aacgcaaccg ggaatattgc tgcggtgcag aaagccgccg aaggattgcc attaacggta
      781 ctgccaggca tggagcttca gacccgggaa gaagttcaca atttatgctt gtttgaaaca
      841 cttgaagaaa ctgccgtgtt tcaagaatgg gtgaataaac acctgccaga tctactcaac
      901 cgcccggatt attttggcga gcagttactg gtggatgaaa acggcgattt tgtggagaag
      961 gagccgcggt tgttattgac ctcagcagat gtttcgattg atgaagcctg tgccaaagtt
     1021 catgaaattg gtggtatatt catacctgca catgtcaatc gaaccgctaa cgggctgctg
     1081 ccgactctgg gatttgtacc agaagggatc tgcctggagg cgctggaaat ttccaagcat
     1141 ttgaaacccg atgaagcccg gcgtaagttt ccgcaaattg ccgggtaccc gctcatccaa
     1201 agtggtgatg tgcaccggct ggatgatttt attggtgtga accagtttga gattgaatcg
     1261 cccactctgg cagagatcaa gctggcgctg cgcggacaag aaggccgcca ataccggata
     1321 ctgtctcgca caatataggt gttttttccc catttatcgc atttgacact tagcaggaat
     1381 tatcaatgac atttgataat tggcagattg aggctgcaag gttacacttt aggcgattag
     1441 gcttaggcaa tttgcacaga actaatctga ttaatatctc acgatggagc gaggaatgcc
     1501 aaacatctta gataaaattc cagcaaacga ccctttgaac gaccccgaaa agaagcgctt
     1561 gattgatgag attctggaag ccaacaagaa tctccctggc gccaccatgg ttgcgctgaa
     1621 tgagctgcaa agccagatcg ggtttgtttc ggagcccatg cagcagtatg tggctcagaa
     1681 actgcgtgtg cctgtcagcc aggtgcacgg cgtgatttcg ttttactctt tcttcaccac
     1741 ccagccccgc ggcaaacaca ccatcaaatt ctgcctggga accgcctgct acgtcggcgg
     1801 cgtgccgcaa ttaatggaaa aagccaagca actgttagga attgaagccg ggcagacaac
     1861 ccccgatggc aacatcaccc tggaagtttg ccgctgcgtt ggtgcatgca gccaggcccc
     1921 ggttattgtg gttgacgagc acgttcacgg gcgcgtcaaa ccaaacaaat tcccgcagtt
     1981 ggtccgctca gttcaggaac cggaagcgta atttgctg
//
LOCUS       DAOPVS010000270         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_60750_length_2011_cov_34.164110, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000270 DAOPVS010000000
VERSION     DAOPVS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60750_length_2011_cov_34.164110"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKL11_11550"
     CDS             complement(<1..425)
                     /locus_tag="PKL11_11550"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNZ14278.1"
                     /translation="MDFVKDIELIQGRSTHKLAFRLDAFLRRTLDILAALGGLLFLAP
                     FFAVIAIVIRRDSPGPVFYRGPRVGLHGKEFGILKFRTMYECPESYAGSAVTTSGDSR
                     ITPLGRWLRDSKINELPQLWNVLVGEMSLVGPRPEDPKLA"
     gene            complement(466..1344)
                     /locus_tag="PKL11_11555"
     CDS             complement(466..1344)
                     /locus_tag="PKL11_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ14279.1"
                     /translation="MTIITPPEQCAHCPHLGLETDPQTHYVVADELHFCHQVSPTQPV
                     NLDYQTRYCLTPDFALCSGYVMGWDRHLPRDVQRKSQHKWDGLQKVLRILAVLLVLII
                     FGALIYIASVTLPDPVAGHSLLTTRTTTPTDTPTPSLTPSMTPLPSHTPTITRTPTQT
                     LTPTPSPTGFYTSTPGPFLETPFGTRQCLVHQVAEGDTIERLSVRYKTTKDVLDAINT
                     AQITPLWIGAYLVICPNQTEVTDLPSFSTQMLSQRILVSDLASQTGIPESDIIAWNNV
                     ETDWLEAGRWVILPQP"
     gene            complement(1362..>2011)
                     /locus_tag="PKL11_11560"
     CDS             complement(1362..>2011)
                     /locus_tag="PKL11_11560"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNZ14280.1"
                     /translation="PVNTLLITSSEPGEGKTSVISNLAVVMAQTGRQVALLDCDLRHP
                     TVHKRFGLQNTDGITHLFQSEQHLNGSCQKTKIENLSILTTGPLPPNPAELLGSRRME
                     TILGMVRENADFVLIDTPPTLAVTDATVLAPIVDGVILVVYPGETHASSARHMVEQLR
                     RVNARVLGVVLNNLNMRSGRYGYHYHYYRSYSAYQDYYGSSGKKRSRSKVTSEVE"
BASE COUNT          486 a          472 c          609 g          444 t
ORIGIN      
        1 gcaagtttag ggtcttcggg acgcggccca accagcgaca tttctccaac cagcacattc
       61 cagagctgcg gaagttcgtt tatcttcgag tcacgcagcc agcggcccag gggggtaatt
      121 cggctgtcgc cgctggttgt aaccgctgaa cccgcatagc tttctggaca ttcatacatg
      181 gttcggaatt ttaagatgcc aaattctttg ccatgaagac cgacccgagg gccgcggtaa
      241 aaaacggggc cgggagaatc gcgccggatg acgatggcaa tgacagcaaa aaacggggcc
      301 aggaagagca atcctcccaa agccgccaga atatccaggg tgcgtcgtaa gaaagcatcc
      361 agccggaaag ccagcttatg ggttgagcga ccttgaataa gttctatgtc ttttacaaaa
      421 tccatgttcg tttccataat atttccccca tcgggcacaa tctatctaag gctggggcag
      481 tatgacccaa cgcccggctt caagccaatc tgtttcgaca ttattccagg caatgatatc
      541 ggactcggga attccggtct gcgacgcaag gtccgagacg agaatgcgtt gggacagcat
      601 ctgtgtggaa aagctgggca aatcagtgac ctcggtttgg ttcgggcaaa tgaccaggta
      661 ggcgccaatc cacagcggtg taatctgagc tgtattgatg gcatccagga catctttggt
      721 ggttttgtaa cggacagaaa ggcgttcaat cgtatcgccc tcggccacct gatgcaccag
      781 gcactgccgg gttccaaacg gggtttcaag gaatgggccg ggtgtggagg tgtaaaagcc
      841 ggttggcgag ggcgtgggag taagcgtttg ggttggtgtg cgggtgattg tgggggtatg
      901 agaaggcagc ggcgtcatgg agggtgtcag gctgggggta ggcgtatctg ttggggttgt
      961 ggttcgggtg gtgagaagcg aatggccggc aacggggtct ggcagggtaa cactggcgat
     1021 atagatcaag gcgccgaaga taatcaatac cagcaggacg gcaaggatcc ttagcacttt
     1081 ctggagcccg tcccatttgt gctgtgattt acgctgcaca tcccggggca gatgccgatc
     1141 ccagcccatg acataaccgg agcagagggc aaaatctggc gtcaggcagt agcgggtctg
     1201 ataatccagg ttgacaggtt gggttgggga aacctgatgg caaaaatgca attcatcggc
     1261 taccacataa tgggtttggg gatcagtctc caaacccagg tgaggacagt gagcgcattg
     1321 ttctggaggg gtgatgatgg tcatggcggc cgataaatcg gttattcgac ctcggaagta
     1381 actttagagc gggaacgttt tttaccgctg gagccgtaat aatcctggta agccgaataa
     1441 cttcgataat agtgatagtg ataaccgtac cgtccgctgc gcatattaag attgttcaag
     1501 acaacgccca gcacacgggc attcacacgc cgcaactgct caaccatatg gcgggctgaa
     1561 ctggcatggg tctctccagg ataaacaacc agaataaccc catccacaat gggcgccaac
     1621 acggtcgcgt ccgttacagc gagggtaggc ggtgtatcaa tcaggacaaa atcggcgttt
     1681 tcgcgaacca tgccaaggat ggtttccatg cggcgagaac ccaatagttc tgccggattg
     1741 gggggcagcg gaccggttgt caatatactc aagttttcaa tcttggtttt ctgacaggag
     1801 ccattcaggt gctgctcgga ctggaataaa tgggtgatgc catcggtatt ctgtaaacca
     1861 aagcgcttat ggacggttgg gtgacgcagg tcacaatcca acagggctac ctgacgcccg
     1921 gtttgggcca tgacgacagc cagattggag atgacactgg ttttgccctc tccgggttcc
     1981 gaactggtta taaggagtgt gttgacgggg c
//
LOCUS       DAOPVS010000271         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_61183_length_2001_cov_55.548304, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000271 DAOPVS010000000
VERSION     DAOPVS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61183_length_2001_cov_55.548304"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1345
                     /locus_tag="PKL11_11565"
     CDS             77..1345
                     /locus_tag="PKL11_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ14281.1"
                     /translation="MPDLTSQLLPQLEARRLNGLDASPTAVYPAYQGQSLVNLPGTIC
                     KLLGAEPLGAPALAPEWLEPLGERYQHVVMLLVDGLGLQYFQRFLDTAPWKDWLNEAA
                     LLPLTSVTPSTTATALTTLWTGATPAEHGILGYEMWLKEYGMVSNMILHCGMTSAGDV
                     GGLRRSGFDPLTFLPVPTLGGHLKAQEVKVSAFTHQSIAYSGLSQMLHRDAELMAYKT
                     LNDLWVTLSDWLDTHADERTYTYVYWGDIDELSHRYSPDDIRPALEFATFAFTLRSFV
                     QQRQMTSHHDTLMLMTADHGQIHTPRQEAFDLRNHPKFTACLTMGPTGENRLPYLFLR
                     SGMEAQAEDYIQKTWPGMFRVFPTETILQSGLMGDDIYALTPQRMGDRVVVPQDHAYW
                     WWANKDNPLQGRHGGLTAQEMLVPLFALPL"
     gene            complement(1442..>2001)
                     /locus_tag="PKL11_11570"
     CDS             complement(1442..>2001)
                     /locus_tag="PKL11_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14282.1"
                     /translation="PMLQWLWSFLKNLFQLLVVGALAVWLIPTWLNRTATQAQKKPFQ
                     SALAGLLAIPAVYIAALVVAGLIILVAIVLGLLALGGLSNLVTGVSFALLAIVVTAFT
                     FLVSLGSKVIVAFLFGRLLMERAFPQAANRNWWALGAGVLVYALVASIPLIGWVIALL
                     VTLVGVGAVWFLYRSHVEAEEIPAL"
BASE COUNT          464 a          531 c          624 g          382 t
ORIGIN      
        1 cagccagtat attgaagcgc tgctgccagg acgataaaac cttttactct ttacagaacg
       61 tttatggaga aatttaatgc ctgacctgac ctcacaatta ttaccgcaac tggaagcgcg
      121 acgactcaat gggctggatg ccagcccaac ggcagtttat ccggcctatc agggacaatc
      181 gctggttaat ttgccaggga ccatctgcaa actgttaggt gcagagccgc tgggcgcccc
      241 ggcgctggcg ccagaatggc tggaaccgct gggggagcgg tatcaacatg tggttatgct
      301 gctggtggat gggttggggc tgcaatattt tcagcgattt ttggatacgg cgccctggaa
      361 ggattggctc aatgaggctg cgctgctgcc gctgacatcg gtaacgccca gcaccacagc
      421 aaccgcgctg accacgctct ggaccggggc aacaccagcc gagcacggga ttttgggcta
      481 tgagatgtgg ctcaaagagt atggtatggt cagcaatatg atcctgcact gtggcatgac
      541 ctcagccggg gatgtgggcg ggctgcggcg cagcgggttt gacccgctga cctttctgcc
      601 ggtgccaacc ctgggggggc atctgaaagc gcaggaggtt aaggtttcag catttactca
      661 ccagagcatt gcctattcgg ggctgtcgca gatgctgcac cgcgatgctg aattgatggc
      721 gtacaagacc ctcaacgatc tatgggtgac gctttcggac tggctggaca cccatgcgga
      781 tgaacgcacc tacacttatg tgtactgggg cgatatcgat gaactatcgc accgttacag
      841 cccggatgat attcgcccgg cgttggagtt tgccacgttt gcattcaccc tgcggagctt
      901 tgtgcagcag cggcagatga cgtctcatca tgataccctg atgctgatga ctgcggatca
      961 cgggcaaatt cacacaccgc gacaggaagc gtttgatctg cgcaaccatc cgaaatttac
     1021 ggcctgcctg acgatggggc ccaccggcga gaaccggctg ccatatctgt tcctgcggtc
     1081 gggaatggag gcacaggcgg aagactacat ccaaaagacg tggccgggga tgttccgcgt
     1141 cttcccgacc gagacgattc tgcaatccgg gctgatggga gatgacatct acgcgctgac
     1201 cccgcagcgg atgggcgacc gggtggtggt gccgcaggat catgcgtact ggtggtgggc
     1261 gaataaggat aacccattgc agggcaggca cggcgggctg acggcgcagg agatgctggt
     1321 gccactgttt gcgctgccgc tttgagatgc taccggagcg ctgaatagtt actggtaaaa
     1381 aacaatccgg agcgggccat acggcaaccg ctccggattt tgttcaattc aaatgtgggg
     1441 gttagagggc tggaatttct tcggcctcaa catgggagcg gtaaagaaac cagaccgcgc
     1501 ccacgcctac cagggtcacc aggagggcaa tcacccagcc gatgagcgga atggaggcaa
     1561 cgagggcata taccagaaca ccggcgccga gcgcccacca gttgcggttt gcagcctgcg
     1621 ggaaggcccg ttccatcaga aggcggccaa acaggaaagc aacgatgact ttgctgccca
     1681 gcgagacgag gaaagtgaag gctgtgacca caatggcgag gagcgcaaag ctgacacccg
     1741 tcaccagatt actgaggccg cccagggcta acaggcccag aacaatggcg acaaggatga
     1801 tcaaacctgc aactaccaga gcggcgatat aaactgccgg gattgccaac aaaccagcca
     1861 gggccgactg gaagggtttc ttctgagcct gcgtggcagt gcggttaagc caggtgggga
     1921 tcaaccagac agccagggca cccacgacca ggagttggaa gaggtttttg aggaagctcc
     1981 acagccattg gagcatgggc g
//
LOCUS       DAOPVS010000272         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_61533_length_1993_cov_64.153251, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000272 DAOPVS010000000
VERSION     DAOPVS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61533_length_1993_cov_64.153251"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="PKL11_11575"
     CDS             complement(<1..1917)
                     /locus_tag="PKL11_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14283.1"
                     /translation="MKLTKRKVLLGLVMLGIILFGCVQARASQPAGALPTPSQTSTQT
                     AAPTASVTPRPTPTAVPSATPSPTPTPVSLPVQFGAPLPDLALEPITAGSIDRLTVVL
                     ERPVVNRLIAAWDGRVTLSVGSYLDVQRSDGSTLRLDSVIGDPILTQPDGRFVLVVGA
                     RQVQVFDARTGALTYERDIALIETGAPVLADELAALSSDGRYLALSLPGSEIVEVIDL
                     KSGDTAAELPGSDPQWAGHGPLFSPQGRWLVIERGGAGILYDTSDWSQAGAFAYLPYN
                     ARRWAWAFSPDESRFVQAVGQQMIVWSLADLKRTDWFSGVPNICELVFSADSRYLAVI
                     DDGCGGPTPDNLETAQVFDTSTGRAVDSPVQQELTSAGVQVIDGHLDISMPEQNEDLK
                     LTVGTQFELPLDNNTIWMVNPVNNPWLICEYTISTGELSCGSEMDVLNYESDSAGFGG
                     WSLDKSLILNTGRYYCGIYDLKTDTAQSYAAAWCSGVFSPDGAYAAVEHSSVQVYAVE
                     SRQVLYQSGSSYKINRDTYSLTDESLVYVTSDLNVMTLNWRSLITGETRAVPLDISEF
                     EFVSPPPKAGTFLRSMAVTTSSSLAAMVTTNGELLIVDAQSGELLYGERLMDSAFWVR
                     FSPDEKLLAVFGNGG"
BASE COUNT          419 a          628 c          581 g          365 t
ORIGIN      
        1 ccccccattg ccaaacacgg ccagcagctt ttcgtctggc gaaaaccgca cccagaaggc
       61 cgagtccatc agccgctcac cgtacagcag ctcgcccgac tgcgcatcca cgatcagcag
      121 ttctccgttt gtcgtgacca tagccgccag gctggatgat gtcgttaccg ccatactgcg
      181 caggaatgtc ccggctttag gcggtggact tacaaattca aactcgctga tatctaaagg
      241 aaccgcccgc gtttctcctg tgatcaggct gcgccagttc agggtcatga cgttcaaatc
      301 ggaagtgaca tacaccaggc tttcgtcagt cagggaataa gtatcccggt tgatcttata
      361 actggatccc gattgatata agacctgtct gctttcaacc gcgtaaacct ggacgcttga
      421 atgctcaacg gccgcatagg cgccgtcagg cgaaaataca ccactacacc aggctgctgc
      481 ataggactgc gcggtatccg ttttgagatc ataaatcccg cagtaataac gacccgtatt
      541 caaaatgagc gatttatcga ggctccaccc gccaaagcca gcggaatcgg actcgtaatt
      601 caggacatcc atttctgagc cgcaagataa ttcgccggtg gatatcgtgt actcgcagat
      661 cagccacgga ttattgaccg ggttgaccat ccaaatggta ttgttgtcca gcggcagctc
      721 gaactgcgtg ccaactgtca gcttgagatc ctcgttttgc tctggcattg agatatccag
      781 atgtccgtca atcacctgaa cccctgccga agtcagctcc tgctgaaccg gcgaatcgac
      841 cgcccggcca gttgacgtat caaacacctg ggcggtctcc aggttatccg gcgttggacc
      901 gccgcagccg tcatcaatca cggccaggta gcggctgtcg gctgaaaaca ccagctcgca
      961 gatgttggga accccgctga accaatctgt ccgcttgagg tccgccagcg accagacgat
     1021 catctgctgg cccaccgcct ggacgaagcg ggactcgtcc ggcgagaagg cccaggccca
     1081 gcggcgggca ttgtagggca ggtaggcaaa cgcgcccgcc tgcgaccagt cggaggtgtc
     1141 gtaaaggatg cccgcgccgc cccgctcaat gaccagccag cggccctgcg gggaaaacaa
     1201 cggcccatgc ccggcccact gcggatcact gcccggcagc tctgccgccg tatcgcccga
     1261 tttcaggtca atcacttcca cgatctctga tccgggcagc gacagcgcca ggtaccgccc
     1321 atctgaagac agggcagcca gctcatccgc cagcaccggg gcgccggttt cgatcagcgc
     1381 aatgtcacgc tcataggtca gggcgccggt gcgcgcgtca aagacctgca cctgcctggc
     1441 gcccaccacc agcacaaagc gcccatcggg ctgagtgaga atgggatcgc caatcacgct
     1501 gtccagccgc agggtggatc catcgctccg ttgaacgtcc agataggagc cgacagacag
     1561 ggtcacgcgg ccgtcccagg cggcgatcag ccggttgacc accgggcgct ccagcaccac
     1621 agtcagccgg tcaatgctcc cggcggtgat gggctccagg gccaggtcgg gcagcggcgc
     1681 gccgaactgc accggcaggg acaccggcgt gggtgtgggt gagggtgtgg cagacggcac
     1741 agccgtgggg gttggcctgg gggtgaccga ggcggttggc gcggccgttt gagttgaggt
     1801 ctgtgatggc gtgggcaggg cacccgcggg ttgagaggcc cgggcctgaa cacagccgaa
     1861 cagaatgatc cccagcatca ccagacccaa cagcaccttg cgctttgtaa gcttcattgt
     1921 gatttcccgt cagccccttt gcggctgcct gcacagatta tcacagggca gagcggtttg
     1981 tcaataggcg cgc
//
LOCUS       DAOPVS010000273         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_61541_length_1993_cov_6.552116, whole genome shotgun sequence.
ACCESSION   DAOPVS010000273 DAOPVS010000000
VERSION     DAOPVS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61541_length_1993_cov_6.552116"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1002..1751
                     /locus_tag="PKL11_11580"
     CDS             1002..1751
                     /locus_tag="PKL11_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNZ14284.1"
                     /translation="MTLVCVPAGDFLMGSTDTDQYASGNEKPQHTVYLDAYWIDQTEV
                     TNTMFGYFVAATGYRTTAEEEGWGYAYDPDYRWGTNGVSGANWMHPSGPSSDLVGKDD
                     HPVVQVTWEDAVAYCNWAGRQLPTEAQWEKAARGTDGRLYPWGNESPDFNRVNRRGGG
                     TTVVGNYPAGASPYGALDMAGNVWELTSDWYDAAYYSSQTDWRNPAGPTSGEYRVERG
                     GGYSISAARVRIIPGFSYSSRGFRCILNAVP"
BASE COUNT          459 a          561 c          557 g          416 t
ORIGIN      
        1 gcgacatcaa gccgcagaac atcatgatca gccccaaaga tggggccgtg ctgaccgatt
       61 ttggcctggt acgggcgatg gaggcgggca gcattggcac ccgcaccgga gccttgatgg
      121 gtaccccggc ctacatggcg ccggaaattt ggcagggcga accggctggg ccagcggccg
      181 atcaatattc cctggcctgt gtgctggtgg agatgctgac cggccggccg ctgtttgccg
      241 gccccacccc gccggcggtg atgcggcagc atttgttgga aggcccgcgc ctgccggaaa
      301 ccttgcgccc ggcctggctc accaatgtga tccggcgggc attggcacaa gacccggcag
      361 atcgctttcc ggacatcccg gcatttgtgg ctgcattaaa gcagcctgat ccactcgcgg
      421 atgcacgatc tgaggtacgc atatcagacg agcaacctgt tttggaacca gagaaaccgg
      481 ccgctaattt gccacatact tcagcaggct tgtccccgga tgcgcctgta cactcagaac
      541 ctttgccggc tgctgccctg aaagctgcat catcccagtc agccacccca ccgccgcccg
      601 attacccacg ctcttctacc caggataggc caaaacccgg ggatcacccc cgcaaaacat
      661 cccccatttc ccgccccagg ctgccagttt gggccattgc gctcatctgt ttgggtggat
      721 ttgcactcct gacgatttta ggcccggggc tcgtcaggac cttgactggt acaccgggca
      781 ccccagcgac ttccgtggcg gttgctaatc cgacgaatac gtctcctcct ccagcgacgg
      841 ctacagttaa accgacaact agacctacaa ccaagccaac tgctaaaccg accagtaagc
      901 cgaccacaaa accgaccagc aaaccgacgg caacgacaga tccaacaatt cccccggcct
      961 gcactgaaat tgggcagatc tggatttcgc cgattgatgg catgaccctg gtctgtgtgc
     1021 cggcgggcga ttttcttatg ggatcgacag atacagatca atatgccagt ggcaatgaaa
     1081 agccccagca cacggtatat ctggatgcct actggattga tcagaccgaa gtgacgaata
     1141 ccatgtttgg ctattttgtg gcggcaaccg gttaccgcac cactgcagaa gaggaaggtt
     1201 ggggatatgc gtatgaccca gactatcgtt ggggaacaaa cggggtttct ggagccaact
     1261 ggatgcatcc atctgggcca agcagtgatt tggttggaaa agacgatcat cccgtagttc
     1321 aagtcacttg ggaggatgca gtggcctatt gtaactgggc gggacgacaa ctgccaactg
     1381 aagcgcagtg ggaaaaagcc gcccggggaa cggatggaag gttgtatcct tggggtaatg
     1441 aatcacctga tttcaacaga gtaaataggc ggggtggtgg taccactgtt gtgggtaatt
     1501 acccggccgg ggcaagcccc tacggtgcgt tggacatggc gggtaatgtc tgggagttga
     1561 cctcggattg gtatgatgcg gcctattatt ccagtcagac cgattggcgt aatccagctg
     1621 ggccaaccag tggtgaatat cgcgtggagc gcgggggggg ctatagtata tccgccgcac
     1681 gcgtccggat aatacctggt ttcagttact ctagtcgggg cttccgctgc atccttaacg
     1741 cagtgccttg aaagaactca ccttaatttc ctctttctga gtctgaatac gtgactgaaa
     1801 atcacaattt ttgaaagtac acaaggagcg tttcctgaca attatcctgt ctgctcaggt
     1861 agcctgagac tgggcgtgcg gggcgaccgg gcatgtcaga ataagtccga caaagcatcc
     1921 caacccagca cgggattcgc agaaactgcc ccggtttttt aatcaactca atgatgccaa
     1981 aatgatacaa taa
//
LOCUS       DAOPVS010000274         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_61989_length_1984_cov_66.447382, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000274 DAOPVS010000000
VERSION     DAOPVS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61989_length_1984_cov_66.447382"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKL11_11585"
     CDS             <1..276
                     /locus_tag="PKL11_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14285.1"
                     /translation="SEIRWTVTRGLAIYGLMGLAYAAYRHLLIPFMAADPSLAQGQNY
                     LGILTTIGLYVIPVGFLVLIVQRIWAHPPLGSSTDEMLYLYRARGKK"
     gene            276..>1984
                     /locus_tag="PKL11_11590"
     CDS             276..>1984
                     /locus_tag="PKL11_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ14286.1"
                     /translation="MTAPVITPGLSILDNGLNIDSGQFQYAWFRLNTPSGERWRCVTL
                     TELVSVPLSMNDDADTLGRQRALVRGLYNAGVEFLYLAMGIFQPEHIGIVQMYGSAGE
                     GATEAEAARQALAGLTAVRAGLANFPQSLTAAPDLNRVRWYLDFVSTRASAIRILLGQ
                     PDPRSSVRSTGRDGGLPESGDDDLALEQNELLFRGMAKIRQSFVFQVTARYIGRQSLT
                     HTLTQIAEVSSNIASRRRGSINIGFSLGIPLMAALSHQVAGSQSAADSQSQSTADGVS
                     HGWGQSHTESESSTVSGATTIGEAHTVSHSSTTGGSISHGTSVSDGVSASTAHSQVET
                     ESSSHTEASGGSTSVSSMVGGGSSSQVSGGTSLTNTESSGTSTSTGTSSGETVANSHS
                     ASLSNGLNVGGSAIIASAGVSYGLTESHGLTMGTSVGESSAVGASEGTSTAEGSSTGW
                     SEGQSSSWGESSGSSSSWSSADTTGHSSSSGVTESQGTSHTEGQSETVSQMWSSSSGE
                     AHTTSRAETSGWAHSRGTADGVSENWGQTHMAGQSAGQAQSRSIAQGWGSSLSTGLMP
                     GMN"
BASE COUNT          368 a          681 c          627 g          308 t
ORIGIN      
        1 tctgaaatcc gctggacggt cacgcgcggc ctggcgatct acggcctgat gggcctggcc
       61 tacgccgctt accggcatct gctgatcccc ttcatggcag ccgacccatc cctggcgcag
      121 gggcagaact acctgggcat cctgaccacc atcggcctgt acgtcatccc ggtgggcttt
      181 ctcgtgctga tcgtccagcg catctgggcg cacccgccgc tgggcagctc aaccgatgag
      241 atgctttacc tgtatcgagc ccgagggaag aaataatgac cgcaccggtg atcacccccg
      301 gcctgtccat tctggacaac ggcctgaaca tcgacagcgg ccagttccag tatgcctggt
      361 ttcggctgaa cacccccagc ggcgaacgct ggcgctgtgt caccctgacc gaactggttt
      421 cggtgccgct gtccatgaac gacgatgcgg acaccctcgg caggcagcgc gccctggtgc
      481 gcggcctgta caacgccggg gtggaatttc tgtacctggc gatgggcatc ttccagccgg
      541 agcatatcgg cattgtgcag atgtacggct ctgccgggga gggtgccacc gaggcagaag
      601 ctgcccggca ggccctggcc gggctgactg ccgtgcgcgc cggactggcg aacttcccgc
      661 agtccctgac cgctgcgccc gatctgaacc gggtgcgctg gtatctggat tttgtctcca
      721 cccgcgccag cgccatccgc atcctgctgg gtcagcccga cccccgcagc tcggtccgca
      781 gcacaggccg ggacggcggc ctgccggagt ctggcgacga cgacctggcc ctggagcaga
      841 atgagctgct cttccggggc atggcgaaga tccgccagtc gttcgtattc caggttaccg
      901 cccgctacat cgggcggcag tccctgacgc acaccctgac ccagatcgcc gaggtgtctt
      961 caaacatcgc ctcgcggcgg cggggcagca tcaacatcgg cttcagcctg ggcatcccgt
     1021 taatggcggc gctgtcgcat caggtggcag gcagccagag tgcggctgat tcccaatctc
     1081 aaagcactgc cgatggcgtc agccacggct ggggccaatc gcacaccgaa agcgagtcca
     1141 gcacggtcag cggcgccacc accatcggag aggcccacac cgtcagccat tccagcacca
     1201 ccggcggcag catctcgcac ggcaccagcg tcagcgatgg cgtctcggcc agcacggcgc
     1261 acagccaggt ggagacggag tcctccagtc ataccgaggc cagcggcggc agcaccagcg
     1321 tcagcagcat ggttggcggc ggctcgagca gtcaggtcag cggcggaaca tccctgacca
     1381 acaccgaaag cagcggcaca tccaccagca ccggaaccag ctctggcgaa acggttgcca
     1441 actcgcattc cgccagcctg agcaacggcc tcaacgtggg cggcagcgcc atcatcgcgt
     1501 cggcgggcgt cagctacggg ctgaccgaaa gccacggcct gacgatggga acctcggtgg
     1561 gtgaatccag cgcagttggc gcctcggagg gcacctccac cgcggaaggt tcttccacgg
     1621 gctggtcaga aggccagtca tcgagctggg gcgagagcag cgggtcgtct tcaagctggt
     1681 caagcgccga taccaccggg cattccagca gttccggcgt caccgaatcc cagggcacct
     1741 cacacacgga aggccaatca gaaaccgtca gccagatgtg gtcttcctct tccggcgaag
     1801 cgcacaccac cagtcgggca gaaaccagcg gctgggcgca cagccgcgga acggcagacg
     1861 gcgtctcaga aaactggggc cagactcata tggcggggca gtctgccggg caggcgcagt
     1921 cgcgctccat tgcccagggc tggggctcca gcctgtccac cggcctgatg ccggggatga
     1981 acat
//
LOCUS       DAOPVS010000275         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_61990_length_1984_cov_63.978227, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000275 DAOPVS010000000
VERSION     DAOPVS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61990_length_1984_cov_63.978227"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1500
                     /gene="rho"
                     /locus_tag="PKL11_11595"
     CDS             226..1500
                     /gene="rho"
                     /locus_tag="PKL11_11595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNZ14287.1"
                     /translation="MNIFDLQTASLADLRQKGRELNIPNANRLKKENLIVRIAQAEGG
                     PEGEEVRGGVLEIMPEGIGFLRPNYQVGAEDVYVSQAQLRRYELRNGDLIIGAARPPR
                     EGEKHYGLLKVDSINGQDPETARSRPNFESLTPIFPDKRFDLETERTILSTRMINLVA
                     PIGRGQRGMIVSPPKAGKTTILKQLANAISSQYPEVHLIMALIGERPEEVTDMDRSVD
                     AEVVASTFDEPVTAHVRMAEITLERAKRLVEMGRDVVILMDSLTRLARAYNLVVNPSG
                     RTLSGGMDPSALYPPKRFFGAARNIEEGGSLTILATALVDTGSRLDDVVYEEFKGTGN
                     MELHLSRRLQERRVFPAIDMERSSTRREDLLLGPDILQRAWLMRRMYMQMITQPPSGA
                     GMDPAVATEAIIQRMGRSRNNQEFLQSLTEEQ"
     gene            1497..>1984
                     /locus_tag="PKL11_11600"
     CDS             1497..>1984
                     /locus_tag="PKL11_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ14288.1"
                     /translation="MSLAKSPKPETPAAADKRNWLGWLLWGLAALMAAVMVFLLVQRF
                     TGPVEAAYTDPDETIEEALPNVEAPLFAPAPEMAGISRPVTPRTIISERGRESVVEYT
                     VEAGDSIFGIAESFDIQPESLLWANAATLNDDPHMIEIGLTLKVPPTDGIVYEWKDGD
                     TV"
BASE COUNT          439 a          521 c          607 g          417 t
ORIGIN      
        1 gggggttgcc aatcatatga ttttcacagc cgcggcgttt gcatctcctt cgggggcgtg
       61 ttataatcca tgtgttcgtt ttcagtgctg cctgagcgcg cctgagtggg taggggcgcg
      121 attgcaggta gatccttcac ctcattgcaa ttccatacaa ttaaagctcc agccctgaac
      181 cggcgctgga gactatgagt ctgtcagata ggcggattaa cgctcatgaa catttttgat
      241 ctccaaactg catcactcgc tgatttgcgc caaaaggggc gagaactcaa catccccaat
      301 gctaaccgtt tgaaaaaaga aaacctgatt gtacgcattg cccaggccga gggcggccca
      361 gagggggaag aagtgcgcgg cggggtgctg gaaattatgc cggagggcat tggctttctg
      421 cgccccaatt atcaggtggg ggcagaggat gtttatgtgt ctcaggcgca gctgcggcgc
      481 tatgaactgc gcaacggcga tctgatcatc ggtgcggcgc gaccgccgcg cgaaggcgaa
      541 aaacattatg ggctgctgaa agtggacagc atcaatgggc aggacccaga aacagcccgc
      601 agccggccca attttgagag cctgaccccg atttttccag ataaacgctt tgacctggaa
      661 actgagcgca caattctctc cacccggatg atcaatctgg tggcgccgat tggccgcggg
      721 cagcgcggaa tgatcgtctc accgcccaag gctggcaaga caacgatttt gaaacagctt
      781 gccaatgcca tttcatccca gtacccggaa gtgcacctga ttatggcctt gattggcgag
      841 cgcccggaag aagtgacgga tatggaccgc tcggtggatg ccgaggtggt ggcttcgaca
      901 tttgacgagc cggttacggc gcatgtgcgc atggcagaga tcacgctgga acgcgcaaaa
      961 cggctggtgg aaatgggccg ggatgtggta atattaatgg attcgctgac ccgcctggca
     1021 cgggcttaca acctggtggt gaacccatcg gggcgcacgc tctcgggcgg catggatcct
     1081 tcggcgttgt acccgcccaa acgctttttt ggtgcggcgc gcaacattga agaaggcggc
     1141 tcgttgacca ttctggcaac ggcgctggtg gataccggct cacggctgga cgatgtggtt
     1201 tacgaggaat ttaaaggtac gggcaatatg gagctgcatc tgtcgcgccg attgcaagaa
     1261 cggcgcgtct tcccggcaat tgacatggaa cgctcctcga cccgacggga agatttgttg
     1321 ttaggccccg atattttgca gcgtgcctgg ctgatgcggc ggatgtacat gcaaatgatc
     1381 acccagccgc cctcaggcgc aggcatggac ccggcagtcg ccaccgaagc catcatccaa
     1441 cgcatgggac ggagccgcaa caaccaggaa tttttacaga gtttgacgga ggaacagtga
     1501 gtttagcgaa atctcccaaa ccagaaacgc cggccgcggc agacaagcgg aactggctgg
     1561 gctggttgct ctgggggctg gcggccctga tggccgcggt gatggtcttt ttactggttc
     1621 aacgctttac cggcccggtt gaggctgctt acaccgaccc cgatgagacg attgaagagg
     1681 cgctgccgaa tgtggaagcg ccactgtttg cacctgcgcc cgagatggcg gggatctctc
     1741 gacctgtgac cccccgaacc atcatttcag aacgggggcg cgagagtgtg gtggaatata
     1801 cagttgaggc cggcgattcc atttttggaa ttgccgaaag ctttgatatt caacccgaga
     1861 gcctgctgtg ggccaacgcc gccactttga atgatgaccc ccacatgatt gaaatcgggc
     1921 tgaccctgaa ggtgcccccc accgatggga ttgtttatga atggaaagat ggcgatacgg
     1981 ttga
//
LOCUS       DAOPVS010000276         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_62762_length_1968_cov_46.193936, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000276 DAOPVS010000000
VERSION     DAOPVS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62762_length_1968_cov_46.193936"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..>1968)
                     /locus_tag="PKL11_11605"
     CDS             complement(29..>1968)
                     /locus_tag="PKL11_11605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ14289.1"
                     /translation="PMKRLEAKLEPWIRSAWTGNLLGWAGILFYLVQAWSNAFTLRSS
                     LDEGNFLVKGYLFATGIFSPYQPYGPWVNKMPLSFLAPGWAQALFDPGLRTGRYFALA
                     LQVLLILGLWLLIRRETGRGWAAAAVWALALAPTQNKIYSMVISQGLTACLLVWTLYF
                     TIGEKRNRWQLLTGSILAGLVVMTRENMLPVVPFLVVYVFWQHGWRMGIVALIGSLLS
                     LGIGHAIFWPDILRNWAKWIPAAVTPFLDPWRVPTAGERTYIQSAGSWFDQLLIAFEG
                     LRIHFAALAGALVAGLLWPRQNQWKQRSHFRAALVLLALLIVLTAAHLWASVGQDYCV
                     YCFPAYLAFFSPLGLIVIVLVLANFQPVLPRRLWRVVGILGAVLIVLIITVGISLSAY
                     SEIARLSAYPAVSDALFHLEVPRMSNGAIQPGTTELWQLFSNKFGLSFEDTRLVLFPR
                     LVAGLAGLLFGLLVLLVSAILTPVLQRRQQPVSFFAVALIAMLLLGTIFSPTSLLAGG
                     QYSFDCESNAVATFESAGRELSHRIASDDQIYWIGSGGVSPAVLLYLPAANIYPPQLN
                     GSYSYRSGGDADELYRYGYWNETLKADWMNASDILLVETRLVEKMQPELDAYQWLPSG
                     APILIANCKMPIYLSVFEKNR"
BASE COUNT          551 a          517 c          552 g          348 t
ORIGIN      
        1 ttatttcggt ctcgggtgtg gtcatgaatt acctgttctt ttcaaagacg ctgaggtaaa
       61 tcggcatctt gcaattagcg atcaatatgg gggcgccgga aggcagccat tgataagcat
      121 ccagttcggg ctgcattttc tcaaccaggc gggtttcgac caacaggata tcggaagcat
      181 tcatccaatc ggccttcagg gtctcgttcc agtagccata gcggtagagt tcatcagcat
      241 cgccgcccga gcggtaggag taagagccgt taagctgcgg cgggtagatg ttagctgccg
      301 gtaagtaaag caaaacagcc ggagagacgc cgccgctgcc aatccagtag atttgatcat
      361 cggaggcgat ccgatgcgag agttcgcgtc cagcggattc aaaggtggct acggcattgc
      421 tctcgcaatc gaaagaatac tgtcctcctg ccaacaggga ggttggagag aagatggtcc
      481 caagcagtaa cattgcgatc aaagcaactg caaaaaacga aactggctgc tgtcgtcgct
      541 gcaggacagg ggtcaaaatg gcgctgacaa gaaggactag caagccgaac agtaagccag
      601 ccagccccgc cacaagccgc gggaaaagga ccaggcgggt atcttcaaat gagaggccga
      661 atttattaga aaacagctgc cagagttctg ttgttccggg ctgaattgcg ccattgctca
      721 tgcgcggcac ttcaagatga aacagtgcgt ctgagactgc gggataggca ctcaggcggg
      781 caatttctga ataggcgctc aggctgatgc ccacagtgat gataagcaca atcaaaacag
      841 cacccaggat acccaccaca cgccataatc ggcggggcag caccggctga aaatttgcca
      901 gcaccaagac aattacgatc aaacccagcg gcgaaaagaa tgccaggtaa gccggaaaac
      961 aatagacaca ataatcctga ccgaccgagg cccagagatg tgctgcggtc aggactatga
     1021 gaagggcaag caacacaagc gccgcgcgga aatggctgcg ctgcttccat tggttttgac
     1081 gcggccaaag caaaccagcg accaaggcgc cagccagggc ggcaaaatgg atgcgaaggc
     1141 cttcaaaagc aatgaggagc tggtcaaacc aactgccggc agactggata taggtacgtt
     1201 cacccgccgt agggacacgc cagggatcaa gaaatggcgt taccgctgcc ggaatccatt
     1261 ttgcccagtt acgcaaaata tcgggccaga agatggcatg accaatgccg agggacagca
     1321 agctgccaat caaggcgacg atgcccatgc gccagccatg ctgccagaaa acgtaaacca
     1381 ccaggaaggg cactaccggg agcatgtttt cgcgcgtcat gactaccaga ccagcaagaa
     1441 tgctgccggt taagagctgc caccgattgc gtttttcgcc gatagtgaaa tacaaggtcc
     1501 agaccagcag acaagctgtg aggccttgag aaatgaccat gctgtaaatt ttattctggg
     1561 ttggggcaag ggccagcgcc caaacggctg cagcagccca gccgcgcccg gtctcacgac
     1621 ggataagcag ccaaaggcca aggatcagta aaacctgcaa tgccagcgca aaatatcgtc
     1681 ccgtgcgcaa gccggggtca aacagggctt gggcccagcc tggagccaaa aacgaaagcg
     1741 gcatcttatt gacccaggga ccgtaaggct gataaggaga aaagatgccc gtcgcgaaaa
     1801 ggtaaccttt gaccaaaaag ttgccttcgt ccagtgatga gcgcagggta aaggcattgc
     1861 tccaggcctg aaccagatag aataatatgc ccgcccaccc cagcaggttt ccagtccagg
     1921 ctgaacggat ccaaggttcc agctttgctt ccaatcgttt cataggcg
//
LOCUS       DAOPVS010000277         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_63571_length_1951_cov_38.225738, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000277 DAOPVS010000000
VERSION     DAOPVS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63571_length_1951_cov_38.225738"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PKL11_11610"
     CDS             <1..1358
                     /locus_tag="PKL11_11610"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ14290.1"
                     /translation="AHGGERSREYTGLVAQHLELAGETVRASILLLQAGQQAAQHFAN
                     SEALQYLDRALALTPAANQSQRFEILMAQENVLNLLGHRATQVSEALDVLQSLAEEMG
                     SPNALAAAALRRSSFAEINGDYPTAIQAGRTAVEQGRLAGNPAQEAEGYYLWGRGLWR
                     QGRLDEALESMDRALEKARPAGVLPVQAGALRTMGAIQFHQGNYSQVFTIYEEAIKLY
                     TEIGDRRGYGSAVNNLGDINRQLGNYARAREYFEQALHTSREIGERWSECIGLLNLCL
                     VAQHQNDPQAALDYAQQALNLAQESGYRSAESFALVGKGNALLALDRLDEALASIQAS
                     IDLRTEMGEQNMVMESRAAMARLRLAQHNLPSALEQVEIILNYLQQNSVDGADEPFRI
                     YLTCCHVLQAAADARYPTVLQEALRLLQAQADRITDPDLHQSFLCNVAAHMELVRLAA
                     A"
     gene            complement(1428..1823)
                     /locus_tag="PKL11_11615"
     CDS             complement(1428..1823)
                     /locus_tag="PKL11_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14291.1"
                     /translation="MWGNKKTEFWANLAPIAFLLIGLGCDWITVLFKNSMTADLSINR
                     NLIIGLQILVNLLFAAAGFGMIQISKVKWTIWGYGVGLIAAVFGLIYPFLPLNIPGLM
                     RIVVSYINLAGAFLAVLLAAKAFTRKQND"
BASE COUNT          423 a          622 c          517 g          389 t
ORIGIN      
        1 tggcccatgg cggcgagcgc agccgggagt acaccggcct ggttgcccag caccttgagc
       61 tggctggcga aaccgtccgc gcctccatcc tcctgctgca ggccggccag caggctgccc
      121 aacactttgc caattctgag gcccttcaat acctcgaccg tgccctggcc ttgactccgg
      181 cagccaacca gtctcagcgg tttgaaattc tgatggccca ggaaaatgtc ctcaatttac
      241 tcggtcaccg cgccacccag gtgagcgaag cgctggatgt actgcaatcc ctggctgaag
      301 aaatgggctc gcccaatgct ttggccgccg ccgcactgcg gcgttccagc tttgccgaaa
      361 tcaacggcga ctatcccacc gccatccagg ccggacgcac cgccgttgag cagggccgcc
      421 tggcgggcaa ccccgcccag gaggccgagg gctactacct gtggggccgg gggctgtggc
      481 gccagggccg cctggatgag gctttggaaa gcatggaccg agctctcgaa aaagctcgcc
      541 ctgccggggt gctgcccgtg caggctggcg ctttgcgcac catgggcgcc attcaatttc
      601 accagggcaa ttattcccag gtgttcacca tctatgaaga ggccatcaag ctttatactg
      661 aaattggcga ccgccgtggc tacggcagcg ccgtgaataa cctgggcgat atcaatcgcc
      721 agttgggcaa ttacgcccgt gcccgcgaat actttgaaca ggcgctgcac accagccggg
      781 aaattggcga gcgctggagc gaatgtattg gcctgctcaa cctctgcctg gtggcccagc
      841 atcaaaatga cccccaggcc gcgctggatt atgcccagca agccctcaac ctggcccagg
      901 aatcgggcta ccgctcggct gaatccttcg ctcttgtggg caagggcaat gcgctgctgg
      961 cgctggatcg tctggatgaa gccttggctt ccatccaggc ctccattgac ctgcgaacag
     1021 aaatgggcga acagaacatg gtgatggaat ctcgcgcggc aatggcgcgt ctgcggctgg
     1081 cccagcataa tctcccctct gctctggaac aggtggaaat tattctgaac tacctgcagc
     1141 aaaattcagt ggatggcgcc gatgaaccct tccgcattta cctgacctgc tgccatgttt
     1201 tgcaggccgc ggcagatgcg cgctacccca ccgtgctgca ggaggctttg cgcctgctcc
     1261 aggctcaggc cgaccgcatt accgaccccg acctgcacca atccttcctg tgcaatgttg
     1321 ctgcccacat ggaactggtt cggctggccg cggcttagcc cttaaaccgg taagttttgc
     1381 tggaagacct tcaatcgtta gaaatccggc ccacccaatc ggtggagcta atcgttttgc
     1441 tttcgagtga atgctttggc tgccagcagc accgccaaaa aggcgcccgc cagattgata
     1501 taagaaacca caatccgcat cagacctgga atattcaagg gaaggaatgg gtagatcaga
     1561 ccgaatactg cggcaatcaa gcccaccccg tacccccaga ttgtccattt gaccttgctg
     1621 atctgtatca tcccaaaccc ggccgcagca aacaacagat tcaccaaaat ttgaaggccg
     1681 ataatcaaat tcctgttgat gctcaaatca gccgtcattg aatttttaaa cagtactgta
     1741 atccaatcac atccaaggcc tataagcagg aaagcgattg gagccagatt ggcccaaaat
     1801 tctgtctttt tattccccca catttattcc cccacactgc gcggcagcga gaagcagatg
     1861 cgcgcaccct gccccacttc cggattgacc cagattcgcc cgccgtgcgc ctcaatcacc
     1921 gcgcgcacca ggtagagccc caatccagcg c
//
LOCUS       DAOPVS010000278         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_64378_length_1935_cov_46.866489, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000278 DAOPVS010000000
VERSION     DAOPVS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64378_length_1935_cov_46.866489"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1737)
                     /locus_tag="PKL11_11620"
     CDS             complement(67..1737)
                     /locus_tag="PKL11_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNZ14292.1"
                     /translation="MLKHPLRFLPIALALAWAVDLFFWNKPLGLSFTLWTLLILGGGF
                     YLIHAEGRRIHWSGWALGALALALSAAPLLRLEELTRFLNVFLTLLALGLSILTARTG
                     YWLYYRLVDWITGGFELIGAAFTRATDIFRSGPAPVGEDDQPVAKPASPWRKLWSVAW
                     RVVLGLMLAFPIVLVLGALLASADPIFANKLEGLLEIFKIDNLPEYIFRFFYVAVLAY
                     LFGGLLVHAAAPHKQAERPDPQKPWMKGFLGPIETGVILAAVDVLFAAFVIVQFGYFF
                     GGATANINATGYTFSEYAVKGFNELVTVAILSLGLYLTLSLINRRETVNHQRSFTWFS
                     VLLFGLVLVMLVSSWYRLGLYEDAYGFTRLRTYTHLFIPWLGALLVVVILLEIFKRRG
                     HFGLALWLFSIGFALTLTLVNVDGFIVRQNTARAVTDGEFSVVSDGGSGGRWLADPDT
                     FDFGYLSNLSHDAVPALLNEYQQDHPAAVRDLLGAQLACRAAAMQTADPRPWIAYNLG
                     EARARQILLDPANQALWSEYPVSHPESWKYEVEVNGETLNCNSWGYAD"
BASE COUNT          440 a          579 c          624 g          292 t
ORIGIN      
        1 tgttccgagg ggttgagggt gctgccgcca aacggctcaa agccggtgat cagcaggcgc
       61 agcggttcag tcggcatagc cccaggaatt gcagttcagc gtctcgccgt ttacttcgac
      121 ctcatatttc caggattcag gatggctgac ggggtactcg ctccacaggg cctgattggc
      181 ggggtcgagc aaaatctggc gggcccgggc ttcgccaagg ttataagcaa tccaggggcg
      241 cgggtcggcg gtttgcatcg cggccgcgcg gcaggccaac tgcgcgccca gcaggtcccg
      301 caccgcagcg gggtggtcct gctgatattc gttcaacagc gccggcacgg catcgtgcga
      361 gagattggag agatagccaa aatcaaaggt gtctgggtcg gccaaccacc gcccgccact
      421 gcctccatct gaaaccacgg agaactcgcc atccgtcacg gcgcgagcgg tgttctggcg
      481 gacgataaag ccatccacat tgacgagggt cagggtcagg gcaaagccaa tcgagaagag
      541 ccacagcgcc aggccaaagt ggccgcggcg tttgaagatc tcgagcagaa tgacgacgac
      601 cagcagggcg cccagccagg ggatgaagag atgcgtgtag gtgcgcaggc gggtgaagcc
      661 gtaggcgtct tcatacagcc ccagccggta ccacgaagag accagcatga ccagcaccag
      721 cccaaacagc agcaccgaga accaggtgaa gctgcgctgg tggttgacgg tctcgcgccg
      781 gttgatcagg ctgagggtca ggtacagccc caggctgagg atggccaccg tcaccagctc
      841 gttgaagccc ttgacggcat actccgagaa ggtgtagccc gtggcgttaa tgttggccgt
      901 ggcgccgcca aagaagtagc caaactgcac aatcacaaag gcggcaaaca gcacatccac
      961 cgccgccagg atcacgccgg tttcaatcgg ccccagaaag cccttcatcc agggtttttg
     1021 cgggtcgggg cgctcagcct gtttgtgcgg cgcggcggca tgcaccagca gcccgccgaa
     1081 caggtaggcc agcactgcca catagaagaa gcggaagatg tactccggca ggttgtcaat
     1141 cttgaagatt tccagcaggc cttccagttt gttggcaaag atcgggtcgg cgctggccag
     1201 cagggcgccc agcaccagca caatcgggaa ggccagcatc aagcccagaa ccacccgcca
     1261 ggccacgctc cacagcttgc gccacggcga agcgggtttg gccacaggct ggtcgtcttc
     1321 gcccaccggg gccggcccac tgcggaagat atcggtggcg cgggtaaagg ccgcgccgat
     1381 cagctcaaag ccgccggtga tccaatcaac caggcggtag tagagccagt agccggtgcg
     1441 ggcagtcagg atgctgaggc ccagcgccag cagcgtcaga aagacgttga ggaagcgggt
     1501 cagttcttcc aggcgcagca gcggcgcggc cgaaagggcc agcgccagcg cgcccagcgc
     1561 ccagccagac cagtgaatgc gccgcccttc cgcgtggatc aaatagaagc cgccgccgag
     1621 gatcagcagc gtccacaggg tgaaggacag gcccagcggc ttgttccaga agaacaggtc
     1681 cacggcccag gccagcgcca gagcaattgg cagaaaacgc aacggatgtt tcaacataag
     1741 gggaatgcct ccagggcagg gatgtcgagc ttataggccc gatgatagac gggcaaggct
     1801 gtcaaaacct atcggaaaat tgcgatagct atcaggatgg gttttggaat attataaccg
     1861 cggtgcgggg tctctaatgg gggatcagga actctcccag gagaaatacc accaccagcg
     1921 cccccgccag caccg
//
LOCUS       DAOPVS010000279         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_64533_length_1932_cov_56.204049, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000279 DAOPVS010000000
VERSION     DAOPVS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64533_length_1932_cov_56.204049"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PKL11_11625"
     CDS             complement(<1..805)
                     /locus_tag="PKL11_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14293.1"
                     /translation="MNHILDIQFPAPFIARPAVLDDAEKATDLFNAISQLLIGANEAI
                     AESVRYYWQTPGFNLETDVLVVFTPDGRLIGEAEFWDTGEPHVHYHGETYVHPDFVNQ
                     GLEIALIAWTEARARRSVGKAPAEALVKIAQGTYDQDHVLKTILQSQGYQYERSFYQM
                     RIEGLTTPPPAPILPDGLIIRAMNPDTEFDAVVDAVADSFQDHWGFVPQPRDERLAQW
                     RHRAAHNPRYDPSLWFVAMDGQEIAAIALCYASTDEDAEMGWVQTLGVRR"
     gene            complement(824..1651)
                     /locus_tag="PKL11_11630"
     CDS             complement(824..1651)
                     /locus_tag="PKL11_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ14294.1"
                     /translation="MASVTLQMRASLAFVERNFNLIKRYWSWELVWLIYSIADCLAVS
                     FIGLGMGALGNATAINTQYMVIYLLIGTLVWRFLSTIFYWITELVSIERWEGTIEYTL
                     MAPIRRFTHMAGQTVFALLYGLAFTGVVLLVTSLVFKIDLSQANLWGGLVMLVAGSFS
                     FVGISIMASVLPLLFPERGAQMTHVFIALLLLVSGVYYPVSVLPGALKALAAYSPATY
                     VLEGVRLALLEGASLASLWPKIWPVLLMGAVLIPLGLFVFQLGERYAKKTGKLHRNG"
     gene            complement(1660..>1932)
                     /locus_tag="PKL11_11635"
     CDS             complement(1660..>1932)
                     /locus_tag="PKL11_11635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNZ14295.1"
                     /translation="AIGEPECWSQGYGADAMRVLMRYAFMELNLHRVNLDVFADNPRA
                     IKSYENAGFVQEGTARAMLCRDGRRWDVVYMGILRSDWDKLHGSPG"
BASE COUNT          455 a          532 c          600 g          345 t
ORIGIN      
        1 ggcggcgcac acccagagtc tgcacccagc ccatttctgc atcttcatca gtagaagcgt
       61 agcagagggc aatggcagca atctcctgac catccatggc gacgaaccac agggaggggt
      121 cgtagcgcgg gttgtgggcg gcgcggtggc gccactgagc cagacgctcg tcgcggggtt
      181 gaggcacaaa gccccagtga tcctggaaag aatcggcaac ggcgtccacc acggcatcaa
      241 actcggtgtc ggggttcatg gcgcggatta tcaggccatc tggcagaatg ggcgcgggtg
      301 gcggggtggt caggccttca atgcgcatct ggtagaagga gcgctcatac tgatagccct
      361 gcgactgcag gatggttttc agtacgtggt cctggtcata ggtgccctgg gcaattttga
      421 ccagggcctc ggcaggggct tttccaacag aacgccgggc gcgggcttcg gtccaggcaa
      481 tcagagcgat ttcgaggcct tgattgacaa agtcgggatg gacataggtt tcgccgtggt
      541 aatgcacatg cggttcaccg gtatcccaaa actcagcttc gccaatcagc cggccatcgg
      601 gtgtgaagac gaccagcaca tcggtttcca ggttgaagcc cggggtctgc cagtagtagc
      661 gcacactttc agcaatggct tcgttggcgc cgattaagag ctgggaaatg gcattgaaca
      721 gatcggtggc cttttcggca tcatccagca cagccggacg agcgataaag ggggccggga
      781 attgaatatc aagaatgtgg ttcatcatga actccttaaa aaatcagcca ttgcggtgaa
      841 gcttgccggt tttcttggca tagcgttcac ccagttgaaa aacgaacagg ccaagcggaa
      901 tcaagactgc gcccatgagc aggacaggcc agattttagg ccacaacgat gcaagggatg
      961 cgccctccaa caaggccagg cgaacccctt ccagaacata agtggcgggg ctgtaagcgg
     1021 ccagcgcctt gagcgcaccg ggcagcacac tgacggggta atagacgccc gaaacaagca
     1081 gcagcagtgc gatgaagacg tgcgtcatct gggcgccgcg ctcggggaag agcagcggca
     1141 gcacagaggc catgatgctg atgccgacaa acgagaagct gccggcgacc agcatcacca
     1201 acccgcccca gagattggcc tgactgaggt caatcttgaa gaccagggag gtgaccagca
     1261 gaacaacccc ggtgaaggcc aggccataga gcagcgcaaa gacggtctgc ccggccatgt
     1321 gggtgaagcg gcggatgggg gccatcaggg tgtattcgat ggtgccttcc cagcgctcaa
     1381 tgctgaccag ttccgtgatc cagtagaaga tggtggacag aaagcgccag accagcgtgc
     1441 caatgagcag gtagatgacc atatactggg tgttgatggc ggtggcgttc cccaacgcac
     1501 ccatgcccag gccaatgaag ctgacggcca ggcagtcggc gatggaatag atcagccaga
     1561 ccagttccca ggaccagtag cgtttgatga ggttgaagtt gcgctccaca aaagccagtg
     1621 aggcgcgcat ttgtagtgtt accgaggcca taatggctcc tatccggggc tcccatgcag
     1681 tttgtcccaa tcactgcgca gaatacccat atagacgaca tcccaccgcc ggccatcgcg
     1741 gcaaagcatg gcacgggcgg tgccttcctg cacaaacccg gcgttttcat aggacttgat
     1801 cgcgcggggg ttatcggcaa aaacatccag gttgacacgg tgcaggttca attccatgaa
     1861 ggcataacgc atcagcacac gcatggcgtc tgcgccgtag ccctggctcc aacattcggg
     1921 ctcgccgatg gc
//
LOCUS       DAOPVS010000280         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_65139_length_1920_cov_27.252547, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000280 DAOPVS010000000
VERSION     DAOPVS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65139_length_1920_cov_27.252547"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKL11_11640"
     CDS             complement(<1..286)
                     /locus_tag="PKL11_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14296.1"
                     /translation="MTKIPKPIFWGMLVIVLTMAACNFPVQPGGQVGNPVWINDPNAT
                     VTPTPFQPMPPTPESPNEMVIPTADAYTPVQTPTAPSADNLIKILVLGSDM"
     gene            complement(330..1799)
                     /locus_tag="PKL11_11645"
     CDS             complement(330..1799)
                     /locus_tag="PKL11_11645"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ14297.1"
                     /translation="MKSLKSILGGLASALITGLIALGALTLSITEGNMWLPAPTEAGP
                     EVTTAAPGETLAIETLSANPTQAGETQILPTPTLPLTAPTTMPGCLPPQGWVQHYIQP
                     GETLADLASQYQITQDTLRSANCLFTDTLLPNTFIYLPPGQIILPTATATTIPCGPPR
                     GWIQITLSAGSTLYSLSRDYNVSVYELQLANCMGYSTQLYAGAKFWVPNVPTRTPTAT
                     EVPLPTHTPPPPTVTLTPTVPSVYGVSLTPAADAQSGDPGTNVSYVLVLTNTGNVSDT
                     YTVSTSGASWTSVAAPSTVGPLAAGAMQNITVTVSIPAGAAGGSTDTVTVTAVSQASG
                     AASDSAALTTTANTLAIYGVSLSPAVDSASGSPGAEVLYTLTVTNTGNTSDYFDLNVS
                     GAAWTTTIAPMTVGPLGAGASEEITVSVSIPDDASNGTTDTATVTAVSQGDTTATATA
                     NLTTTASIKAGLGLPIQWAETGTFPATTASHNTDSRQAL"
BASE COUNT          360 a          548 c          563 g          449 t
ORIGIN      
        1 gcatgtctga ccccagcacg aggattttga tcaggttatc tgcacttggt gctgttggag
       61 tctgcaccgg ggtgtatgca tccgccgttg ggatgaccat ctcgttgggg ctttctggcg
      121 ttggcggcat gggttgaaac ggcgttggcg ttaccgttgc atttgggtcg ttaatccaca
      181 ccgggttgcc cacctgtcct cccggctgaa ctggaaaatt acaggccgcc attgttaaaa
      241 caatgaccag catcccccaa aaaatgggct tgggtatttt agtcatagtg cgcgcaaatc
      301 ctatccacta attctatcgc cggacgcggt tacagcgcct gtcgcgagtc tgtattatga
      361 gacgctgtcg ttgcaggaaa tgttccagtt tcagcccact ggatgggcaa acccaatcct
      421 gctttgatgc tggcggtggt cgtcaggttt gccgttgctg tggctgtagt atccccctga
      481 gatacggctg tcactgtagc cgtgtctgtg gtcccatttg acgcatcatc tggaatgctc
      541 acgctgacag taatctcctc gcttgcgccg gcacccagcg gcccaaccgt catgggcgca
      601 atcgtggtag tccacgccgc cccactgacg ttcagatcaa aataatcgct ggtgttacca
      661 gtattcgtca cagtcagggt gtagagtacc tccgcacccg ggcttcccga tgccgagtcc
      721 acagccggac tcagcgacac tccatagatt gccagggtat tggctgtggt cgtcagggct
      781 gccgaatcgg atgctgcccc actggcttgc gataccgctg tcacggtcac tgtatcggtg
      841 cttccacctg ccgcgcctgc cggaatgctg actgtgacgg tgatattctg cattgcccct
      901 gctgccagcg gccccacggt agatggcgcc gccacgctcg tccagcttgc cccgctggtc
      961 gagacggtgt aagtgtcgct gacattcccc gtattggtca aaaccagcac atagctgacg
     1021 tttgtgcccg gatcgccact ttgtgcatcg gccgccgggg ttaaagaaac cccataaacc
     1081 gatggaaccg tcggcgtcaa agtcactgtg gggggcggag gggtatgagt aggcagtggc
     1141 acttccgtcg cggtgggggt gcgggttggc acattcggca cccagaactt ggccccggca
     1201 taaagctgcg tgctgtatcc catacagttt gccagttgca gttcataaac tgaaacattg
     1261 taatcccggc tcaagctgta gagcgtgctt cccgcgctta acgtaatctg aatccacccg
     1321 cgcggcgggc cgcagggaat ggttgtggca gttgcggtgg gcaaaataat ctgcccgggc
     1381 ggcaggtaga taaatgtatt gggcagcagc gtgtcggtga ataagcaatt ggcgcttcgc
     1441 agcgtatcct gcgtaatctg atattggctt gccaaatctg ccagggtttc accgggctgg
     1501 atataatgct gcacccagcc ttgcgggggc aggcagcctg gcatggtggt cggcgccgtc
     1561 aaaggcagcg tgggggtggg cagaatctgc gtctctcctg cctgggttgg gttggcggac
     1621 agggtttcaa ttgccagcgt ctcgcccggt gcggcggttg tcacctccgg tccggcttcg
     1681 gttggcgctg gcagccacat attcccctcg gtgattgaaa gcgtcagcgc tcccagggca
     1741 atcaaccctg tgatcagcgc cgatgccaaa ccacccagga tagacttcag cgatttcatt
     1801 cgcctagaaa tcttccaatc tgccggggtt tttcaatcgg cagcagcacg ctgaaggtcg
     1861 aactttgtcc aatttcgctc tcaacccaga tgcgcccgcc gtgcgcctca accagcgctt
//
LOCUS       DAOPVS010000281         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_66379_length_1897_cov_43.704669, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000281 DAOPVS010000000
VERSION     DAOPVS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66379_length_1897_cov_43.704669"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKL11_11650"
     CDS             <1..467
                     /locus_tag="PKL11_11650"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNZ14298.1"
                     /translation="SITGDTWMIAGQTVRTTAATEIKDGATVGDNVKVHATLQADGTY
                     LAREIEKSMGGGSPTETPGGSEVEFYGVVASITGDTWMIAGQTVRTTAATEIKDGATV
                     GDNVKVHATLQADGTYLAREIEIDTDNGGDDNGNDDNGGDDNSGDDDDDDDD"
     gene            582..1106
                     /locus_tag="PKL11_11655"
     CDS             582..1106
                     /locus_tag="PKL11_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ14299.1"
                     /translation="MNSELLTTPQALGQLHDEHYPAVYRYLAYRLGDPALSEDLAAET
                     FLRLLAALRKPLTRVDNPRAWLLGTAHHLAMDALRRKYRRREEDLADHESLPAHHSPE
                     AAAETAQTLRQVRQAMRRLTADQQHVLALRFTQELSLEETAQMMKKSLSAVKVIQFRA
                     LAALRRLLLEADIR"
     gene            1103..>1897
                     /locus_tag="PKL11_11660"
     CDS             1103..>1897
                     /locus_tag="PKL11_11660"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HNZ14300.1"
                     /translation="MKTPDFSTHLDAALNQLNAGQDLEQILAAHPAQAAELRPLLQAA
                     RSARLPAAAQPDRLAQNRSRARFLAAAQSQTHRRASFSLGRLALNALAALLLVIVTLG
                     LGGLASVQALPGDSLYPVKQALERAQLALSSGAQNRLTLEEQFDSRHAAEVEQLIVRG
                     SSGQMVSFAGVLTLGAVDGFWVAGIPLQLSPDLLASAFNLMGAYVELDGTLLPDGHVQ
                     VSQMQLRLFNLQGRLQQTSASTWLVDGVRVEIAPQTQILGQPQAGQR"
BASE COUNT          382 a          624 c          535 g          356 t
ORIGIN      
        1 cctccatcac aggcgatacc tggatgattg ccggtcagac tgtgcgcacc accgccgcca
       61 ccgaaatcaa agacggcgcc accgtgggcg ataacgtcaa agttcacgcc accctgcagg
      121 ccgatggcac ctacctggcc cgcgaaattg aaaaatcaat gggtggcggc agccccactg
      181 aaacccctgg cggcagtgaa gtcgaattct acggcgtcgt tgcttcaatc acaggcgata
      241 cctggatgat cgccggtcag actgtgcgca ccaccgccgc caccgaaatc aaagacggcg
      301 ccaccgtggg cgataacgtc aaagttcacg ccaccctgca ggccgatggc acctacctgg
      361 cccgcgaaat cgagattgac actgataatg gcggtgacga caatggcaat gacgacaacg
      421 gtggtgacga caacagcggc gacgatgacg atgatgatga cgactagccg ttggatttct
      481 ggcaccttac cagactgaga ttgaaattga gatgctcctc cggcaaatca ctaccggagg
      541 agcatccccg ctccgacttt ttgacggtat actcaatgct gatgaactcc gaactgttga
      601 ccacccccca ggcgctgggc caactgcatg acgagcatta ccccgccgtg taccgctacc
      661 tggcctaccg cctgggcgac ccggcattga gcgaagacct ggcggctgaa acctttttgc
      721 ggcttttagc tgctctgcgc aaaccgctga cgcgcgttga taacccccgg gcctggctgc
      781 tgggcaccgc ccaccatctg gccatggatg ccctgcggcg caaataccgc cgccgcgaag
      841 aagatctggc cgatcacgaa tcgttacccg cccaccactc ccccgaagcc gccgccgaaa
      901 ccgcccaaac cctgcgccag gttcgccagg ccatgcgccg cctgactgcc gatcaacagc
      961 atgtgctggc cctgcgcttt actcaagagc tctcgcttga agagacggcc caaatgatga
     1021 aaaaatcgct gagtgccgtc aaggtcatcc aattccgcgc ccttgccgcg ctgcgccgat
     1081 tgttactgga agctgatatc cgatgaaaac ccctgatttt tccacccatc tggatgctgc
     1141 gcttaatcaa cttaacgcgg ggcaagacct ggagcaaatt ctggctgccc accccgccca
     1201 ggcggctgaa ctgcgcccct tgctgcaggc cgcgcgctca gcccggctgc ccgcggcagc
     1261 ccaacccgac cggctggccc agaatcgcag tcgcgcccgg tttttagccg cggctcagtc
     1321 acaaacccac cgcagagcct ccttttcatt gggacggctg gcactcaatg ccctggcggc
     1381 tttgctgctg gtcatcgtca ccctgggcct gggcgggctg gcctctgtcc aggcgctgcc
     1441 cggtgattca ctttacccgg tcaaacaggc cttggagcgg gctcaattgg cgctttccag
     1501 cggcgcgcag aaccgcctga cgcttgaaga gcagtttgac agccgtcacg cggccgaagt
     1561 tgagcagttg attgtccgcg gcagcagcgg tcaaatggtc agctttgccg gcgtcttgac
     1621 cctgggcgcc gtagatggct tctgggtggc tgggatcccc ctgcagcttt ccccagatct
     1681 gttagccagc gcctttaacc tgatgggcgc ctatgtggaa ttggacggca cgctgctgcc
     1741 cgatggtcat gttcaggtca gtcagatgca gctccgcctg tttaatcttc aggggcgtct
     1801 ccagcagacc agcgcatcaa cctggctggt ggatggcgtg cgggttgaga ttgccccgca
     1861 aacgcagatt ttgggccagc cccaggcggg ccaaagg
//
LOCUS       DAOPVS010000282         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_66623_length_1892_cov_54.271639, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000282 DAOPVS010000000
VERSION     DAOPVS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66623_length_1892_cov_54.271639"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1892
                     /locus_tag="PKL11_11665"
     CDS             <1..>1892
                     /locus_tag="PKL11_11665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNZ14301.1"
                     /translation="LIRDAFRRSKYQDIILPFTVLRRIDCVLEPTKTAVLQAHARYKS
                     ELPNLDPVLRKASGFVFYNTSPFTFERLTDDPANLAANLQAYINGFSDNMREVIEKFD
                     LRNTIAKLDEAGLLFLVVERFKNIDLHPDQVDNHAMGTIFEELIRRFNEALDENPGEH
                     FTPREIIHLMVDLLVGPENGKLLEPYRVIKVADPACGSGGMLTLAKTRLLAANPTLQV
                     YLYGQEVNPETFAICKADLYMKSPDGKDAENIAYGSTLSAEGHPNERFNYQLANPPYG
                     KEWKVDEEKVRQEAAKPGGRFSAGLPRVSDGQLLFLQHMLSRMAPPEDGGGRVAIVMN
                     GSPLFTGDAGSGESEIRRWILQNDWLEAIIALPEQLFYNTGIATYIWVLSNRKAEHRR
                     NQVQLIDATAIWTSMRKSMGDKRREITNDHIAEIYQLYARFESNERCKIFATTDFGYR
                     KVTIERPLRLNFEATPERLQRLTEQPAFAGLAQSRKKDPAEKASEDAAGRARQAQILA
                     QLARLPQTRFTDRPAFEAALDALGAKFEAPIRKAILTALSERDETAAICYAKDGSPEP
                     DPDLRDTENVPLGEDIETYFAREVAPHLPDAWINTTLCDPQDGRVGRVGYEINFNRYF
                     YTYQPPR"
BASE COUNT          398 a          598 c          528 g          368 t
ORIGIN      
        1 ctgatccgcg atgcttttcg ccgcagcaag tatcaagata tcatcctgcc gttcacggtg
       61 ctgcgccgca ttgactgcgt gctggagccg accaagaccg ctgtgctgca ggcccatgcc
      121 cgatacaaga gtgaactgcc caatcttgac cccgtgctgc gcaaggcttc gggctttgtt
      181 ttctacaata cctcgccctt tacctttgag cgcctgaccg atgaccccgc caacctggcg
      241 gccaatctgc aggcctatat caacggcttc agcgataata tgcgtgaggt gattgaaaag
      301 tttgacctgc gcaacaccat cgccaagctg gatgaggccg ggctgctctt tctggtggtc
      361 gagcgcttca aaaatattga tctgcacccc gaccaggtgg acaaccacgc gatgggcacc
      421 atcttcgagg aactgatccg ccgctttaac gaggcgctgg acgagaaccc cggcgaacac
      481 tttacgccgc gcgagattat tcacctgatg gtggatctgc tggttggccc tgaaaatggc
      541 aagctgctcg aaccctaccg cgtgatcaaa gtggccgacc cggcttgcgg ctctggcggc
      601 atgttgacgc tggccaaaac ccgcctgctt gcggccaacc ccaccctgca ggtgtacctt
      661 tacgggcaag aggtcaaccc cgagacgttt gccatctgta aagctgacct gtatatgaag
      721 agccccgacg gcaaagatgc cgagaatatc gcctatggca gcacattatc tgctgagggc
      781 caccccaatg agcgcttcaa ttaccagctt gccaacccgc cttatggcaa agagtggaag
      841 gtggatgagg aaaaagtgcg gcaggaagcc gccaaacccg gtgggcgctt cagcgccggg
      901 ctgccgcggg tgagtgatgg gcagctgctc tttttgcagc acatgctcag ccgcatggcg
      961 ccgccagagg atggcggcgg ccgcgttgcc attgtgatga acggttcgcc gctctttacc
     1021 ggcgatgccg gcagcggcga aagtgaaatc cggcgttgga ttttgcagaa cgactggctc
     1081 gaagccatca ttgccctgcc cgagcagctt ttctacaaca ccggcatcgc cacctatatc
     1141 tgggtgctta gcaaccgcaa ggctgaacac cggcgcaacc aggtgcagtt gattgacgcg
     1201 accgccattt ggacatccat gcgcaagagc atgggcgaca aacgccgtga gatcaccaac
     1261 gaccatatcg ccgaaattta ccagctttat gcgcgctttg aatccaatga gcgttgtaag
     1321 atctttgcca ccaccgattt tggctaccgc aaagtgacca ttgagcgccc cctgcgcctg
     1381 aattttgagg ccacccccga gcgtttgcag cgcctgaccg agcagccggc ctttgccggg
     1441 ctggcgcagt ctcgcaagaa agaccccgcc gaaaaagcca gcgaagatgc cgccggacgc
     1501 gcccgccagg cccagatttt ggcgcagttg gcgcgtttgc cgcaaacccg ctttaccgac
     1561 cgccccgcct ttgaagccgc cctggatgcc ctgggcgcta aattcgaggc ccccatccgc
     1621 aaggccattt tgaccgccct gtctgagcgg gatgaaactg ccgccatttg ttacgccaaa
     1681 gacggcagcc ccgagcccga ccccgacctg cgtgataccg agaatgtgcc cctgggcgaa
     1741 gacattgaga cctattttgc ccgtgaggtg gccccgcacc tgcccgatgc ctggatcaac
     1801 accaccctgt gtgacccgca ggatggccgc gtgggccggg tgggctacga gatcaacttc
     1861 aaccgctatt tttacaccta ccagccgccg cg
//
LOCUS       DAOPVS010000283         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_67492_length_1876_cov_48.334981, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000283 DAOPVS010000000
VERSION     DAOPVS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67492_length_1876_cov_48.334981"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..694
                     /locus_tag="PKL11_11670"
     CDS             71..694
                     /locus_tag="PKL11_11670"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HNZ14302.1"
                     /translation="MVAIYHITHQNNLANILATGGLWCDAQTSANGLRPTEIAHANIK
                     ERRSRRRVPVSMDGVLADYVPFYFCPRSPMLYSIHHGYVTAYQDGQAPLLHLRTSTEA
                     VADCELRFAFTDGHATMALSRYFDDLTDLNQLDWEVINSRYWSDTASDNDRKRRKQAE
                     FLVYRFFPWNLVQEIGVINRQMADVVTNLIQNQAHQPIVSICRNWYY"
     gene            710..1741
                     /locus_tag="PKL11_11675"
     CDS             710..1741
                     /locus_tag="PKL11_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNZ14303.1"
                     /translation="MIKLVSGNLLEAQVEALVNTVNTVGVMGKGVALQFRETFPENYK
                     AYQKACRLNEVQIGKMFVFTTGLLSLPRYIINFPTKRHWRDKSRLEDIEAGLFDLVQV
                     IKQHNIHSLALPPLGCGAGGLNWNDVRPLIEQALSGLTDTTVLLYPPAGSPRPDEIRP
                     QTPKADLNLGRAALVYLVDQYALPAHRLTLLEVQKLMYFLQEAGQPLKLNYVRQLYGP
                     YAENLNHVLQRLEGHYLSGYGDRTGRATIRAVPGAKAEAETFLSEDPETLQRLKDVLK
                     LIEGFETPYGMELLSTVHWLARENPAVKTDYQLAVQGFESWNRRKREHFRPEHIKVAW
                     ERLRQQGWI"
BASE COUNT          470 a          504 c          475 g          427 t
ORIGIN      
        1 taccgcgccg ccctcatctc ggccgcggtc accgggaaaa tggatgtaca caggcctgca
       61 ggataaccca atggtcgcga tttaccatat tacgcatcaa aataaccttg ccaatatcct
      121 tgcgacgggt ggtctgtggt gcgatgccca aacatcagcg aatggcctcc gaccgacaga
      181 aattgcccat gcaaacatca aagaacgccg ctcccggcgc agggtgccgg tttctatgga
      241 tggtgtattg gctgattatg tgccttttta tttctgcccg cgctcgccca tgctctattc
      301 aattcatcat ggttatgtaa cagcttatca ggacgggcag gccccgctat tacatctcag
      361 gacctcaacc gaggctgtgg ctgattgcga actgcggttt gcctttaccg atgggcatgc
      421 aacgatggcg ctcagccgct attttgatga cctgaccgac ctgaatcaac tggattggga
      481 ggtcatcaac tcgcggtatt ggagcgatac tgcctcggac aacgatcgga agcgccgcaa
      541 acaggccgaa tttctggtat atcgcttttt cccctggaat ctggtgcaag aaattggggt
      601 tatcaaccgg caaatggcag atgttgtgac caacctcatc caaaaccagg cccatcagcc
      661 gattgtcagc atctgcagaa attggtatta ttaaacaaag gcagtcccca tgataaagct
      721 tgtttctggc aatttacttg aagcgcaggt cgaagccctg gtcaacaccg ttaacactgt
      781 aggcgtgatg ggcaagggcg ttgctttgca attccgcgaa acctttcccg aaaattacaa
      841 agcgtatcaa aaagcttgtc ggctgaacga agtgcagatc ggcaaaatgt tcgtcttcac
      901 caccgggctg ctcagtctac cgcgttacat catcaacttt cctaccaagc gccactggcg
      961 cgataaatct cgtctggaag atattgaagc cgggttgttt gacctggtgc aggtgatcaa
     1021 gcagcacaat attcattcgc tggccctgcc gccgctgggc tgcggtgctg gcggtctcaa
     1081 ctggaatgac gtgcgcccat tgattgagca ggctctcagc gggctaacag acacaaccgt
     1141 tctgctttac ccaccggctg gctcaccccg acccgatgaa attagacccc aaacaccaaa
     1201 ggcagatcta aacctcgggc gggcggcgct tgtatatctg gtagatcaat atgcactgcc
     1261 ggcccaccgc ttgactttgt tggaagttca aaaactgatg tatttcttgc aggaagccgg
     1321 gcaacccctg aaattgaatt acgtccgaca actttatggc ccctacgccg aaaacctgaa
     1381 tcatgtattg cagcgcctgg aagggcacta cctttcaggt tatggcgatc ggaccggacg
     1441 ggctactatt cgcgctgtgc caggggcaaa agccgaagcc gaaacatttt taagcgagga
     1501 tcccgagacc ctgcaacgcc tgaaagacgt tctaaaattg attgaggggt ttgaaacgcc
     1561 ctacggcatg gaactgctct ctacagtcca ctggttggcg cgggagaatc cggccgtaaa
     1621 aaccgattac cagcttgctg tacaaggttt tgaaagttgg aaccgccgca aacgcgagca
     1681 ttttcgccca gagcatatca aggttgcctg ggaaagacta cgccaacagg ggtggatcta
     1741 aacttaacct ggcgcagaca ttcagatttt tcttgacgtc accgtcacct ggcaatcaga
     1801 ccggggaaaa ggcaggggct aatgcccaca atctctgaaa agaattttga agaaaccatc
     1861 gaatccgccc tgctga
//
LOCUS       DAOPVS010000284         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_67719_length_1872_cov_54.104568, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000284 DAOPVS010000000
VERSION     DAOPVS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67719_length_1872_cov_54.104568"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1869
                     /gene="uvrC"
                     /locus_tag="PKL11_11680"
     CDS             <1..1869
                     /gene="uvrC"
                     /locus_tag="PKL11_11680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNZ14304.1"
                     /translation="APMKAIPERIQQILDTLPTKPGCYIMHNAAGEIIYVGKAINLRN
                     RVRSYFHDSAGHEAKTRQLVRQIADIEWIVVGSELEALILEMNLIKKHRPRFNVRLKD
                     DKRYPYIKVHWNTPFPKVTVTRQMVEDGARYYGPYTSVWAVHQTLDVLRRIFPYLTCD
                     REITGKDARACLYFDIKLCSGPCIGVVDQAAYRAMIDDLCQFLEGRTEAVVERLRTQM
                     EQAAEDLRFERAAALRDQITAIDRVVEKQKVVSSEKIDSDVIAMARSEGEACVQIFFI
                     RGGKLIGREYFILEGAAEAADSEVIGQFLKQFYAEAATVPCQVMLPNEIEEARIIEQW
                     LSSRPNCSKVEISVPRQGPAQELMEMAAENATETLRALRAQWEADTNRQTEALAELQQ
                     ALRLSEPPNRIECYDISNTQGTASVGSMVVFEQGVPAKGMYRRFNIQTVQGPDDFASM
                     EEVLTRRFRRWQTLQTRAEAPGDKPDPSFARLPDLLIVDGGKGQLARAVAVLERFELT
                     GRVPVVGLAKQQEELFLPGQRLSVMLPRHSQGLYLVQRIRDEAHRFAITAHRKARTRQ
                     GLASRLDAIPGIGPARRKQLLQRFGSLEGIQQASLEDLMSLKGITEEIARHLKDNL"
BASE COUNT          369 a          615 c          585 g          303 t
ORIGIN      
        1 gcgcctatga aggccattcc cgagcgcatc cagcagattc tggataccct gcccaccaag
       61 cccggctgct acatcatgca caacgccgcc ggcgaaatta tctatgtggg caaggccatc
      121 aacctgcgca accgggtgcg ctcttacttc cacgacagcg ccgggcacga ggccaaaacc
      181 cgccagctcg tccgccagat cgccgacatc gaatggattg tggtcggctc ggagctggaa
      241 gcgctgatcc tggaaatgaa cctgatcaag aagcaccgcc cgcgcttcaa cgtccggctg
      301 aaagacgaca aacgctaccc ctacatcaag gttcactgga acaccccctt ccccaaggtc
      361 accgtcaccc ggcagatggt ggaagacggc gcccgctact acgggcccta taccagcgtc
      421 tgggcagtgc atcagaccct cgatgtgctg cggcgcatct tcccctacct gacctgtgac
      481 cgtgagatca ccggcaaaga tgcccgcgcc tgcctgtact ttgacatcaa actgtgcagc
      541 gggccgtgca ttggcgtggt cgatcaggcc gcctaccggg cgatgattga cgacctgtgc
      601 cagtttttgg aaggccgcac cgaggcagtg gtggagcgcc tgcgcacgca aatggagcag
      661 gccgccgaag acctgcgctt tgagcgcgcc gccgccctgc gcgatcagat caccgcaatt
      721 gaccgggtgg tggaaaagca gaaagtggtc tccagcgaga agatcgactc ggatgtgatt
      781 gccatggccc gctctgaagg cgaggcctgc gtgcagattt tcttcatccg cggcggcaag
      841 ctgattgggc gtgaatattt catcctggag ggcgcggccg aggccgccga cagcgaggtg
      901 attgggcagt tcctcaagca gttctacgcc gaggcggcca ccgtgccctg ccaggtgatg
      961 ctgcccaacg agatcgaaga ggcccgcatc atcgagcagt ggctgagcag ccgccccaac
     1021 tgcagcaagg tggagatcag cgtgccgcgc cagggccccg cccaggagct gatggaaatg
     1081 gccgccgaaa acgccaccga aacgctgcgc gccctgcgcg cccagtggga ggccgacacc
     1141 aaccgtcaga ccgaggcgct ggcagagctg cagcaggccc tgcgcctgag cgaaccgccc
     1201 aaccgcatcg aatgttacga catctccaac acccagggca cggcctcggt gggcagcatg
     1261 gtggtttttg agcagggcgt gcccgccaag ggcatgtacc gccgcttcaa cattcaaacc
     1321 gtgcaggggc cggatgattt tgccagcatg gaagaagtgc tgacgcgccg cttccgccgc
     1381 tggcagaccc tgcaaacccg tgccgaggcc cccggcgaca agcccgaccc ctcttttgcg
     1441 cgcctgcccg atctgctgat tgtggacggc ggcaaggggc agctggcgcg ggcggtggct
     1501 gtgctggagc gctttgagct gaccggccgc gtgccggtgg tggggctggc caaacagcag
     1561 gaagagctct tcctgccggg gcagcgcctc tcggtgatgc tgccgcgcca ttcgcagggg
     1621 ctctacctgg ttcagcgcat ccgagatgag gcccaccgct tcgccatcac cgcacaccgc
     1681 aaagcccgca cccgtcaggg gctggcctcg cggctcgatg ccatccccgg catcggcccg
     1741 gcccgccgca agcagctgct gcagcgcttt ggcagcctgg agggcattca gcaggcctca
     1801 ctggaagatc tgatgagcct gaaaggcatc accgaggaaa ttgcccggca tttgaaggat
     1861 aatttatgag gc
//
LOCUS       DAOPVS010000285         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_67958_length_1868_cov_57.301710, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000285 DAOPVS010000000
VERSION     DAOPVS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67958_length_1868_cov_57.301710"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..753
                     /locus_tag="PKL11_11685"
     CDS             166..753
                     /locus_tag="PKL11_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNZ14305.1"
                     /translation="MIQSGFTSLQTLTQAVITLAPLTPIPVEQLIARPVALWAPNWLL
                     LTSGDFAAGHYNTMTVAWGTLGMMWNLPVAQVVVRPVRYTYEFMEQYPTFTLTAFPGK
                     YRKALTLLGARSGRGFDKIAAAGLTPIAASQAAAPSFAEATLLIECRKIYSQDFDPAR
                     FLDARIPRQYPQKDYHRVYYGEILAVSGTEQYLAP"
     gene            889..>1868
                     /locus_tag="PKL11_11690"
     CDS             889..>1868
                     /locus_tag="PKL11_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ14306.1"
                     /translation="MTTETVQKTKLLTPLLLLFMVTMILANLGGSMYGPLEALYLKDL
                     GAGIGQIGLFYTLSQIIPLALQILGGWISDSLGRLRAIAIGSVVGVLVYIPLLFATRW
                     EWVLVASAFSAVTRSLIGPSFDAFIADHSAPENRARLFGITQTLFGIVSVVGPIAGGY
                     LVEWINFKGMLAVAAALYLIATFMRIGMAREAARGHEANPAALTFSSLKTNLGAMVVL
                     ILAGGLFTWILITDGVRDIFFGMSMSFLSVYMQDIASLTISQIGFMNSIFGLAMMAVM
                     IPAGWLADKVGERVNIAISFLLLAFSIGMIALIPPQSPAWVYAIGWTIAG"
BASE COUNT          343 a          646 c          453 g          426 t
ORIGIN      
        1 agcggctacg agcgatcaac cttcttaaac gggagttttt tcattcagct tcaagatggc
       61 tgggtcttca tgcccgaaca ggccttcccc gaactggtgg ggcatgccat gtcgttctat
      121 catctggagg gcgttcccta gccgctcaag acccagaatc caggtgtgat acaatcaggc
      181 ttcacctcat tacagaccct tacacaggca gtcatcacct tggccccgct cacacccatt
      241 cctgtcgaac aattgatcgc ccgtccggtt gccctgtggg ccccaaactg gctgctgctc
      301 accagcggcg attttgccgc cgggcattac aacaccatga ccgttgcctg gggcaccctc
      361 ggcatgatgt ggaatctacc cgtcgcccag gtggtggtcc gtccagtgcg ctacacctac
      421 gaattcatgg agcagtaccc caccttcacc ctcaccgcct tccccggcaa ataccgcaag
      481 gcgctcaccc tgctgggcgc ccggtctggc cgcggttttg ataaaattgc cgccgctggc
      541 ctcaccccca tcgccgcctc gcaggcagcc gccccgtcct ttgccgaagc caccctgctc
      601 atcgaatgtc gcaaaatcta ctcacaagat tttgaccccg cccgcttcct ggatgcccgc
      661 atcccgcgcc agtatcccca gaaggactat caccgggtct attacggcga aattcttgcc
      721 gtcagcggca cagagcagta tctcgccccg taaaaatcac cctgccatcc tgcccgcgcc
      781 cgccaggcca gattgtaaaa aatccgcgca cttcctcaaa aaacgcagta tttgcattat
      841 gattacacct tgcgtttaat cctcgtcacc tgccaggtag ccgccccaat gacaactgaa
      901 accgttcaaa aaaccaaatt attaaccccg ctcctgctcc tgtttatggt caccatgatc
      961 ctggcgaacc tgggcggcag catgtatggc ccgctggaag ccctttacct caaagacctg
     1021 ggcgctggca tcgggcaaat tggcctcttc tacaccctct cgcagatcat cccgctggcc
     1081 ctccagatcc tgggcggctg gatttcagat tccctgggcc gcctgcgggc catcgccatc
     1141 ggcagtgtgg tgggcgtttt ggtctatatt ccgctgctgt tcgccacccg ctgggaatgg
     1201 gtgctggtag cttccgcctt cagcgccgtc acccgctcgc tcatcggtcc cagctttgat
     1261 gccttcattg ccgaccattc cgcccccgaa aaccgcgccc gcctcttcgg catcacccag
     1321 accttgttcg gcatcgtctc cgtagtcggt cccattgccg gcggctatct ggtcgaatgg
     1381 attaatttca aaggcatgct ggctgtagcc gccgcgctct acctcatcgc cacattcatg
     1441 cgcattggca tggcccgcga agccgcccgc ggtcacgagg ccaaccccgc cgcgctgacc
     1501 ttcagcagcc tcaaaaccaa cctgggcgcc atggttgtcc tgattctggc tggcggcctg
     1561 ttcacctgga ttctcattac cgatggcgtg cgggatattt tctttggcat gtccatgagc
     1621 ttcctctccg tctatatgca ggatattgcc agcctcacca tcagtcaaat tggattcatg
     1681 aacagcattt tcggcctcgc catgatggcc gtcatgatcc ctgctggctg gctggccgat
     1741 aaggttggcg agcgcgtcaa cattgccatc tcgttcctgc tgctggcctt ctccatcggc
     1801 atgatcgcgc tcatcccgcc ccagagcccg gcctgggtct atgccatcgg ctggaccatt
     1861 gccggcat
//
LOCUS       DAOPVS010000286         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_68602_length_1857_cov_43.354051, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000286 DAOPVS010000000
VERSION     DAOPVS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68602_length_1857_cov_43.354051"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKL11_11695"
     CDS             <1..980
                     /locus_tag="PKL11_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ14307.1"
                     /translation="VQNLTKTYRVPIRPEGLSASLKSLLRPRYSEVTAVNALSFSIEP
                     GAMVGLIGPNGAGKTTTLKMLSGLLHPSAGTGTVAGHVPWQRHPDYLRRISMVMGNKS
                     QMLWDIPPMDSFRVLSEVYAVPPAEFRRTLDELIALLDLQELLTKPVRTLSLGERMKC
                     ELTASLLHRPTVLFLDEPTLGLDVSMQLRLRRFLAEYNQRSGVTIILTSHYMADVLAL
                     CPRVILIHHGRLLYDGELNGLAHKLLPFKMLRITLGSETVSPENLPPLPPGAEVAEIE
                     SNILTLRVPRQDAPAITARLLQTLPVADLTVEDPPIEAVIDQIYTEGAA"
     gene            977..1765
                     /locus_tag="PKL11_11700"
     CDS             977..1765
                     /locus_tag="PKL11_11700"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNZ14308.1"
                     /translation="MIRSGLALVRGLWLSWLQYRSFFFILAFGWMISPLIYLFVWSTA
                     AGDSSIAGLTRGEFVAYYLVLIIVNQVTYAQTNWTVGDLIRYGQMNHILLRPIPPIYD
                     ALASEFAGKVVYLIFDIPIVALLALILRPEMDFHLINGLAFIPALFLAWALRFFWGYW
                     LALLAFWATRADALLALQDSLIFLLAGQVAPVALLPGLMQTFAKILPFRYMVAFPVEV
                     LTGQLPPAELVVGFAAQAGWLIIAFALYVILWRAGLRRYTAVGG"
BASE COUNT          307 a          650 c          464 g          436 t
ORIGIN      
        1 aggttcaaaa cctcaccaaa acctaccgcg tcccaatccg ccccgagggg ttatccgcct
       61 cattgaaaag cctgctgcgc cctcgctaca gtgaggtcac cgcagtcaat gctctcagct
      121 tctccatcga gcctggcgcc atggtcggtc tcatcggtcc caatggcgcc ggcaagacca
      181 ccacactcaa aatgctctct ggcttgctgc atccctccgc cggaacagga accgttgccg
      241 ggcatgttcc gtggcagcgc caccccgatt atctgcgccg catcagcatg gtcatgggca
      301 ataaaagcca gatgctctgg gatatccccc ccatggactc tttccgcgtc ctcagcgaag
      361 tctatgctgt gccccccgcc gagtttcgcc gtacgctgga cgaactgatt gccctgctcg
      421 acctgcagga actgctgacc aaacccgtac gcaccctctc gctgggggag cgcatgaagt
      481 gcgagctgac cgccagcctg ctccaccgtc ccaccgtctt attccttgat gaacccaccc
      541 tcggccttga tgtttcgatg cagctccgcc tccgccgctt tctcgccgaa tacaaccagc
      601 gcagcggcgt gaccatcatc ctcaccagtc actacatggc cgatgtcctg gccctctgcc
      661 cgcgcgtcat cctgattcac cacggccggc tgctctacga tggcgaatta aacgggctgg
      721 cccacaagct gctgcccttc aaaatgctgc gcatcaccct cggctcagaa accgtttctc
      781 ccgaaaacct gcccccgctg ccgcccggcg ccgaagtcgc cgaaattgaa tccaacatcc
      841 tgacgctgcg cgttccccgt caggacgcgc ccgccatcac cgcccgcctg ctgcaaaccc
      901 tgcctgtcgc cgacctgacg gtcgaagatc cccccatcga agctgtcatt gaccagattt
      961 acacggaagg tgccgcatga tccgcagtgg cttggccctc gttcgcggcc tgtggctgtc
     1021 ctggctgcaa taccgcagct tcttcttcat cctcgccttt ggctggatga tttccccgct
     1081 gatctatctg ttcgtctggt ccaccgccgc cggtgatagt agcattgctg gcctcacccg
     1141 tggcgagttt gtggcctact atctggtgct gatcatcgtt aatcaggtca cctatgccca
     1201 gaccaactgg accgttggcg acctgatccg ctacgggcag atgaatcaca tcttgctgcg
     1261 tcccatcccg ccaatctatg atgccctggc ttccgagttc gcaggcaagg tggtctatct
     1321 gatttttgat atccccattg ttgccctgct ggcattaatc ctgcgccccg agatggattt
     1381 tcacctgatc aatggcttgg ctttcatccc ggcgcttttc ttggcctggg ctttgaggtt
     1441 cttctggggc tactggctgg ccctgctggc cttctgggcc acccgtgccg atgccctcct
     1501 cgcgctccaa gactcattga tcttcctgct tgccgggcag gtggcgccag tggccctgct
     1561 ccccggcctc atgcagacct tcgccaaaat cctgcccttc cgctacatgg tggctttccc
     1621 cgtcgaagtg ctgacgggtc agcttccccc ggcagagctg gtggttggtt ttgctgcaca
     1681 ggctggctgg ctgattatag ccttcgccct gtatgttatc ctctggcgcg ctggcttgcg
     1741 ccgctacact gcagtaggag gttgaaatgc gcccctttcg tctgatcgcc gctttcatgc
     1801 gggcttcctt tcaagaggaa tccgcctacc gcgccaattt tttcatcagc ctgctca
//
LOCUS       DAOPVS010000287         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_69139_length_1848_cov_43.433352, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000287 DAOPVS010000000
VERSION     DAOPVS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69139_length_1848_cov_43.433352"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1848)
                     /locus_tag="PKL11_11705"
     CDS             complement(170..>1848)
                     /locus_tag="PKL11_11705"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNZ14309.1"
                     /translation="EFELPGVADYWYDPINISFSPDGQYFAASHSKGEDVYEFKLWQT
                     HDWSLVQAQSSFDCFDFSPDGQSIVVSDSIQSEILIYRISDWTLTARQKIQDDWRTPT
                     FMSVIYSEDGQKVIASPYKQTLQVFDATDLTPLYHLGVEYMINTVGLDRITHKTCDAA
                     LIDSCRGLYTPRIISTEALRRVPAHNAIAALYRGDIDWQIQLYSLEDGSPLHQYDATG
                     ATGFDFSPDGRFLAISHDDTGTVDIWDTQAEQLVSTLTAFNSPVKALQFSSNGAWLAT
                     TNGAAMTFIRRMQDGAVLRGIAGVVGFSPSGEQIMTGLPNGQVTVEAISGAQPGGLVT
                     ETGALEAVAYSHNGQLVFSSTSECNLRVRQANDGSLIRTLEPPRISDEIVSSPVRVPV
                     TRLLSSPDGQYLFGSDEFGTLTIWNLADGSQVRLNTVPTEQGGLFRSPLSMALSPDGQ
                     TLAFADLESIVLYSPARQQILAMIPTPQSGAQAPFDITPVAFSPDGSLLVVGVSDGSL
                     YVLNAQTLEIIRRFQAHDNPGGLLALAFSPDGRLLATSSQDGTIRLWGIR"
BASE COUNT          434 a          519 c          501 g          394 t
ORIGIN      
        1 cactggtcat cctgggggcc tggcggcggc agcaacacca gccctcaaag tagggaggag
       61 agggttccac gttccaggtt cgccgcagcc gtggcggtct gctatccctg ggagcagctt
      121 ctataaatgg gatagttaca gcctggttgg gtacggccca ggctcatccc taacggatgc
      181 cccataaccg gatggtgcca tcctggctgc tggttgccag caggcgcccg tctggcgaaa
      241 aggccagcgc cagcaggccg ccgggattat catgcgcctg gaaccgccgg atgatttcca
      301 atgtttgggc attaagcaca tagaggctgc catcagaaac gcccacgacc agcaggctgc
      361 cgtcagggga gaaggcaacc ggagttatgt cgaaaggcgc ttgggccccg ctttgaggtg
      421 ttggaatcat cgccaggatt tgctgcctgg ccgggctgta tagcacaatc gattccaggt
      481 cggcaaaagc cagcgtctgc ccatccggtg acagcgccat gctcagcggc gagcggaata
      541 accccccttg ctctgttgga acggtattca agcgcacctg gctcccatcc gccagattcc
      601 aaatcgtcag cgtgccaaat tcatcagagc cgaacaggta ctgcccatca ggtgaactga
      661 gcaaacgggt taccggaacc cgaacgggag acgacacaat ctcatccgaa attctggggg
      721 gctccagggt acggatgaga gacccatcat tggcctgccg gacacgcagg ttacactccg
      781 aagtggatga aaaaacgagc tgcccattgt gtgaataagc cactgcctcc aaagcgcccg
      841 tttcagttac cagcccgccg ggctgcgccc ccgagatcgc ctcaacggtc acctgtccgt
      901 tcggcaaacc ggtcataatc tgttcgccgg acggcgaaaa ccccacaacg ccagcgatgc
      961 cacgcagcac cgccccatcc tgcatccggc ggatgaatgt catggctgct ccattggttg
     1021 tcgccagcca ggcgccattt gacgagaact gcagggcctt taccggagaa ttgaacgctg
     1081 tcagcgtgct gacaagctgc tcggcttgcg tatcccagat atccaccgtc ccggtatcgt
     1141 catggctgat ggccaggaat cgcccatctg gcgaaaaatc gaatcctgtg gccccagttg
     1201 catcgtattg atgaaggggt gatccatctt ccagactgta caattggatt tgccagtcaa
     1261 tatcgccccg gtatagggcc gcaatcgcat tatgggcagg gacccgtcga agcgcctctg
     1321 tgcttatgat tcttggggta tataggccgc ggcaactgtc aatcagggcc gcatcacagg
     1381 ttttatgggt tatgcggtca agacctacag tgttaatcat atactcaacg ccaagatgat
     1441 aaagtggcgt caagtccgtc gcatcgaaga cttgcagcgt ttgtttgtaa gggctggcaa
     1501 taactttctg tccatcttca gaataaatta cgctcatgaa tgtcggcgtt cgccaatcgt
     1561 cttggatttt ctgccgcgcc gtaagggtcc aatctgaaat tcggtaaatc agaatttcag
     1621 attgaatact atcgctgacg acgatggatt gcccatccgg agagaaatca aagcagtcaa
     1681 aactggactg ggcctgcaca aggctccaat catgtgtttg ccagagttta aattcatata
     1741 cgtcttcccc tttggaatgg gaagcggcaa aatactgccc atctggcgaa aatgaaatat
     1801 taatggggtc ataccaataa tctgccacac ctggcagctc aaattcct
//
LOCUS       DAOPVS010000288         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_69717_length_1838_cov_49.038699, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000288 DAOPVS010000000
VERSION     DAOPVS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69717_length_1838_cov_49.038699"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="PKL11_11710"
     CDS             <1..1757
                     /locus_tag="PKL11_11710"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ14310.1"
                     /translation="LACGWVRVLSLTEIAAEIEKSLDFLNTGQRNLPERHRSLRGVFD
                     YSWQLLDNPRRQALRRLSVFHNPAERAAAQTVAGATLADLSALLDKSLLRRTAAGYYD
                     MHPLIRQYAGERLISNPVEDQATHQAHCNWFVSAAKDWQSDLSGPDQGQVLEAIERVQ
                     QDLRAAWDWAVQQSDCGALKVLLQPLYRFYTVRARYGEGLAALRSALPMVEKIPSQPT
                     LRLLLMNRIAALLICQDNYTEAEPIARAALLLARQLDVPAEIAFSLIQLAVGHWMSGD
                     YEEATSAYHASLEIANQSGAQTMRLEALNGLGKTSWATGQSDQATDYFETGLALARQM
                     NAPLQIAHSLDLLGVVARDSGNFSRAMQCFEEAINILRPLQAPTRLAYALNHLGGVYG
                     ISGQMDRAISTLNESLTMAREINEQRLTAYTLSDLGAMYMNMDQYDQAETSQQDARNI
                     FHQIGDVFGEAIVETSLADLALRRGDKATSARFVCSALKIGLETGILYRLEGLLVTGA
                     ILLQRRGLFQPALQILETIQEQIKTQSGEKVDLTPLIAEITAELDEDAIHQAQVAARS
                     LDFESAARLLQQQLCDAP"
BASE COUNT          386 a          559 c          491 g          402 t
ORIGIN      
        1 agctggcctg cggttgggtg cgggtgctca gcctgacaga aattgccgcc gagattgaga
       61 aaagcctcga tttcctgaac actggccaac gcaacctgcc tgaacggcac cgcagcctgc
      121 gcggtgtctt cgactactcc tggcagctcc tggataatcc ccgccgtcag gctcttcggc
      181 gattgtccgt tttccataac cctgcagaac gcgccgccgc ccagaccgtt gccggagcca
      241 ccctggccga tctttccgcc ctgcttgata aatcgctgct gcgccgcacc gctgccggct
      301 attatgacat gcaccccctc atccgtcagt acgccggcga acgcttgata tcaaaccctg
      361 tagaagatca ggccactcat caggcgcact gtaactggtt tgtttcagcc gccaaggatt
      421 ggcaatctga cctcagcggc cccgaccagg gtcaggtttt ggaagccatt gagcgcgtac
      481 aacaagattt gcgcgccgcc tgggattggg ccgtgcagca atccgattgt ggcgcactga
      541 aagtgttatt gcaacccctc taccgcttct atactgtccg ggcgcgctac ggagagggtc
      601 tcgccgctct tcggtcagcg ctgccaatgg ttgagaaaat ccccagccag cccaccctgc
      661 ggctcttgct gatgaatcgc atcgcagcct tacttatctg tcaagataat tacactgaag
      721 ccgagcccat tgcccgggca gccttgctgc tggcgcgcca gttggatgtg cctgcagaaa
      781 tcgccttcag cttaatccag cttgcggtgg ggcattggat gagcggcgac tatgaagaag
      841 ccacctcagc ttatcatgcc agcctggaga tcgccaatca gtctggcgca caaaccatgc
      901 gcctcgaggc gctcaatggg ctgggcaaga catcctgggc taccgggcag tcagatcagg
      961 ctacagatta ttttgaaact ggcctggcgt tggcccgcca gatgaatgcc ccgcttcaga
     1021 ttgcccacag cctggattta ctgggcgtcg ttgcccgtga ttcgggaaac ttctcccgcg
     1081 caatgcaatg cttcgaagaa gccatcaaca tactgcgccc tttgcaggcg ccaactcgcc
     1141 tggcttacgc gctgaatcat ctgggcggcg tgtatgggat cagtggtcag atggatcggg
     1201 ccatttcaac cttgaacgaa tccttaacca tggctcgcga aatcaatgag cagcgcttga
     1261 ccgcttacac gctttcagac cttggcgcta tgtatatgaa tatggatcaa tatgaccagg
     1321 ccgaaaccag tcagcaagat gcccggaata tattccatca gattggtgat gtttttggcg
     1381 aagcaattgt cgagaccagc cttgccgacc tggcactgcg ccggggcgat aaggcgactt
     1441 cagccaggtt cgtttgttcg gcgctcaaaa ttggtctgga gacgggcatc ctctaccgcc
     1501 tggagggctt gctggtcacc ggggccattc tcctgcagcg tcggggcctg tttcaaccag
     1561 ccctccagat actggaaacc atccaggaac aaatcaaaac ccaatctggc gaaaaagtgg
     1621 atctaacccc tctgattgct gaaattaccg ccgagctgga cgaagatgca attcaccagg
     1681 cccaggttgc cgcgcgctcg ctcgactttg aatccgccgc ccggcttctt cagcagcagc
     1741 tttgtgatgc cccttaaaca gcagccccgc cctcaattgc actgggcggg ctgtgttata
     1801 atttctgtgc cgaaggtggg aatcgaaccc acactcct
//
LOCUS       DAOPVS010000289         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_70208_length_1830_cov_33.554366, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000289 DAOPVS010000000
VERSION     DAOPVS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70208_length_1830_cov_33.554366"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKL11_11715"
     CDS             <1..322
                     /locus_tag="PKL11_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNZ14311.1"
                     /translation="AMGMLLRIKPIIVLRDGLLQMSEKVRTRSQSLDHIIQRTRERIK
                     NQPVDLAIVHAAAPEAAQELLQKVRQFFNVRDVIVTQLSLPVAANLGRGAVGIVAYPV
                     LEEG"
     gene            325..555
                     /locus_tag="PKL11_11720"
     CDS             325..555
                     /locus_tag="PKL11_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14312.1"
                     /translation="MGSADSSIDALEASLRSSLRTIQPDPVFVGHLHERLIARPVGPS
                     MEARAPSLPLFALLGATLMGLLFIWLYRRSRD"
     gene            complement(558..1181)
                     /locus_tag="PKL11_11725"
     CDS             complement(558..1181)
                     /locus_tag="PKL11_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNZ14313.1"
                     /translation="MTRQAGKSYNDGMFSQSTRETIQAHLESAQQSRQAGNEGRARVS
                     ARLAAAESIRAYVRAQRLVIPATSAYDLLLWLRDLPTASEAIRRSAEALTLRVDIHFK
                     LPTGVDLIAETQNLVELLEQESSMTETGEIVIYGTSWCGHSRRSRGIFDQNGIAYRWV
                     DIDQDMEGRKFVESVNHGNRSVPTIVFPDGSVLTEPSDMTLRAKLGL"
     gene            1258..1737
                     /locus_tag="PKL11_11730"
     CDS             1258..1737
                     /locus_tag="PKL11_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ14314.1"
                     /translation="MSEARQVAHQLLHTIPLVMRNTAARMRVAAAGLEPPHFRLLGLL
                     SRRSYTLGELAEQQSVKPATISKTIATFVERGWVQRREVPHDRRLVQVEITPEGRQVI
                     KDAHQQMEAEVTTLLAGLSPQELDQLQVGLSLLQRLCDTPDDPWPCKFESDLADPEE"
BASE COUNT          370 a          579 c          457 g          424 t
ORIGIN      
        1 cgcaatgggg atgttgctgc gcattaaacc catcatcgtg ctgcgggatg gcctgttgca
       61 gatgtctgaa aaagtgcgta cccgcagcca gtcgctagat cacatcatcc aacgcacccg
      121 tgaacgcatc aaaaaccagc ctgtggacct ggcaattgtc cacgccgccg cgcccgaagc
      181 cgcccaggaa ctgctgcaga aggtgcgcca gttctttaat gtgcgtgatg tcattgtcac
      241 ccagctttca ctgcccgtgg ctgcaaattt gggccgcggt gctgttggca ttgttgccta
      301 ccccgtttta gaggagggat aaccgtggga tctgctgatt catccatcga cgccctggaa
      361 gccagcctgc gcagctcgct tcgaaccatc caacccgacc ctgtatttgt cgggcatttg
      421 cacgaacgtc tgattgcccg tccggttggc ccctcaatgg aggcccgcgc gcccagcctg
      481 ccattgtttg ccctgctcgg cgcaaccctg atgggcctgc tttttatttg gctttaccgc
      541 cgctcccgcg attagcgtta cagccccagc ttagctcgca gcgtcatgtc cgaaggctca
      601 gttagaacgc tcccatccgg aaacacaatc gtcggcacac tgcggtttcc gtgattgacg
      661 ctttcaacaa actttcgccc ttccatatcc tggtcaatat ccacccagcg gtaggcaatc
      721 ccgttctgat caaaaatgcc ccgtgaccgc cgcgaatgac cgcaccagct ggttccataa
      781 atcacaatct cacctgtctc tgtcatgctt gactcctgtt ctaataattc caccaggttt
      841 tgagtttctg caattaagtc caccccggtg ggtagtttga agtggatatc cacacgcagt
      901 gtcagcgctt cggccgatcg ccggatggcc tcggaggctg tcggcaggtc tcgcagccac
      961 agcagcaaat cgtacgcgct ggtcgccgga atgaccaatc gctgcgcccg cacatacgcc
     1021 cgaatggatt cggccgcggc cagccgcgca ctcacgcgtg cccggccctc attgcctgcc
     1081 tgtcgggatt gttgcgctga ttctaaatgc gcctgaatcg tctcccgcgt ggattgactg
     1141 aacatgccat cattgtagct tttgccagct tgccttgtca agcgcactgt tcaacctctt
     1201 gacaggcaaa atatccctga tataatttcg cactgctaaa tattaggagt acgaaacatg
     1261 tcagaagccc gtcaagttgc tcatcaattg ctgcatacca tccctttggt catgcgcaac
     1321 accgcggccc ggatgcgggt tgccgcagcc ggccttgagc ccccgcattt ccgcctgttg
     1381 ggactgttgt cccgccgttc ctacaccctc ggtgaacttg ccgagcagca gtccgtcaag
     1441 ccagccacga tttcaaaaac cattgccacg tttgtcgagc gcggctgggt ccagcggcgc
     1501 gaggtgcccc acgaccgccg gctcgttcag gtcgaaatca ccccggaggg ccgccaggtg
     1561 ataaaagatg cccatcagca gatggaggcc gaggtgacca ccttgcttgc cggtctatca
     1621 ccccaggaat tggatcaact tcaagtgggc ctgtctttgc tgcagcgcct gtgcgatacc
     1681 cccgatgacc cctggccctg caagtttgaa tccgaccttg ccgaccctga agaataatat
     1741 ctatctgata acctcgtaat cttgccgatt gtgttacaag gagccccaca ttcatgctta
     1801 aactgttcaa atatctgaag ccttacctcg
//
LOCUS       DAOPVS010000290         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_71678_length_1806_cov_61.701314, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000290 DAOPVS010000000
VERSION     DAOPVS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71678_length_1806_cov_61.701314"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1198)
                     /locus_tag="PKL11_11735"
     CDS             complement(14..1198)
                     /locus_tag="PKL11_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ14315.1"
                     /translation="MANPGRRRRSGETLSMLLEKADVQGYLTTDDLMEACPDVSGDAE
                     RLEAIMVALRHRGVDILDRDNEASFPEDESLLDVADDAYPASAMESVSSDDTIGLYLK
                     EMSRVPLLNMDEELSLARQIEQGKQARRLLERCNSRLTPARRHELEQQAEAAVTAREH
                     LIKANTRLVVSIAKRYMGRGVPFLDLIQEGNLGLMKAVEKYEYQRGFRFSTYATWWIR
                     QTISRAIADQGRTIRVPVHMVDRIRLLYRQTHELEQQLGRIPTSDELAGAMQVSINKV
                     EWMQRVSWLPLSLESPVNEDEEDSELGMFVEDQQTPTPTQSAYELLLREKIEEVLETL
                     PAREARILRLRFGLENGHTYTLEEVGQKFGLTRERIRQIESKALRRLRHPRRARQLKE
                     YL"
     gene            complement(1259..1723)
                     /locus_tag="PKL11_11740"
     CDS             complement(1259..1723)
                     /locus_tag="PKL11_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNZ14316.1"
                     /translation="MKQPFPFRVNIGFLLNQPIGTRRDIHFDVPAFQLSIDLEAQAIT
                     GVTRLTRTGQGILVDSQFDASVPADCVRCLVNFLQPLHISFTDLFAFNPDQATESGLL
                     VPDDGNIDLAPLVMEYLILEIPIQPICRPECKGLCPVCGADLNTAPCEHSAA"
BASE COUNT          341 a          527 c          548 g          390 t
ORIGIN      
        1 tgaaagctga aacttacaaa tattctttca actgccgggc gcggcggggg tgccgcaggc
       61 ggcgcagggc tttgctttca atctggcgaa tgcgttcacg cgtcaggcca aacttttgcc
      121 ccacttcttc cagggtgtag gtgtggccgt tctccagccc aaagcgcagc cgcagaatgc
      181 gcgcctcgcg ggcgggcagc gtttcgagca cttcttcaat cttttcccgc agcagcagct
      241 cgtaggcgct ctgggtgggc gtgggcgtct gctgatcttc tacaaacatg cccagttcgc
      301 tgtcttcttc gtcttcgttc acggggcttt ccagcgaaag cggcagccac gagacccgct
      361 gcatccactc caccttgttg atggagacct gcatggcgcc ggccagctca tcgctggtgg
      421 ggatgcgccc caactgctgc tccagctcgt gcgtctggcg gtacagcagg cggatgcggt
      481 ccaccatgtg gaccgggacg cggatggtgc ggccctggtc ggcaatggcg cgcgagatgg
      541 tctggcggat ccaccaggtg gcataggtgg aaaagcggaa gccgcgctga tattcgtatt
      601 tttccacggc cttcatcagg cccaggttgc cctcttgaat cagatcgagg aagggcacgc
      661 cgcggcccat gtagcgcttg gcaatgctga ccaccagccg ggtgttggcc ttaatcagat
      721 gctcgcgggc ggtcacggcg gcctcagcct gctgctccag ttcatggcgg cgggcgggcg
      781 tcaggcggct gttgcagcgt tccagcagac ggcgggcctg cttgccctgc tcaatttggc
      841 gggccagcga aagctcctcg tccatattca acagcggcac gcgcgacatc tctttgaggt
      901 agagcccaat ggtatcgtct gaagagaccg attccatcgc actggcgggg taggcgtcat
      961 cggccacatc cagcaggctt tcatcttccg ggaagctggc ctcgttgtcg cggtccagaa
     1021 tatccacccc gcggtggcgc agcgccacca taatcgcctc cagccgctcg gcatcccccg
     1081 acacatcggg acatgcttcc atcaaatcat ctgtcgtcaa gtagccctgc acgtctgctt
     1141 tttccagcag catgctcagg gtttcgccgc ttcttctccg cctgcctggg ttagccattc
     1201 tacaagtcct cccgatggtt ttctccgcct gcgtcgtttc tcggcatagt ggctccgctc
     1261 aagccgccga gtgttcgcag ggcgcagtgt tgaggtcggc tccgcaaacc gggcacagcc
     1321 ccttacattc ggggcggcag atcggttgga tggggatttc gaggatgagg tattccatca
     1381 ccaggggagc caggtcaata ttgccgtcgt ccggcaccag cagccccgat tcagtcgcct
     1441 gatcggggtt aaaggcaaac aggtctgtaa aagatatatg caaaggttgc aagaaattga
     1501 ccaggcagcg cacgcagtca gcgggcacgc tggcatcaaa ctggctgtcc accaggattc
     1561 cctgaccggt gcgggtcaac cgggtgacgc cggtgatggc ctgcgcttca aggtcaatgg
     1621 agagctggaa agcggggaca tcaaaatgga tgtcgcgccg cgtgccaata ggctgattca
     1681 ataaaaagcc gatgttgact cggaagggga agggttgttt cactgtttag tagggattgc
     1741 ccgaagatta atagcccata atgcgtatat attatagcat ggcgattcgg gcaagtcaat
     1801 tattta
//
LOCUS       DAOPVS010000291         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_72787_length_1789_cov_35.513264, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000291 DAOPVS010000000
VERSION     DAOPVS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72787_length_1789_cov_35.513264"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKL11_11745"
     CDS             <1..942
                     /locus_tag="PKL11_11745"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNZ14317.1"
                     /translation="QQGRADTLTRVTLLPQPGDHWLYAIQPGGCGEQLNPLTELDVIF
                     PVLHGSFGEDGTLQGLLEMANIAYVGAGVLGSALGMDKAVFKDVMRTCGVPVLNSVTV
                     TRQQIRQGLNDVIAAAEQMSAYPLFTKPANLGSSVGITRCRGRADLVEGLQEAARYDR
                     RILVEQGLDHAREIEVSVLGNEQPQVSVPGEILPCDDFYSYEAKYLDDRSRLVIPADL
                     PAEVTAQVQAYALTAYQAVDCAGMARADFLLEPKTLAIYLNEINTIPGFTQISMYPKL
                     WQASGLPYPALVDRLIELALERKAENDLTEREYRRAA"
     gene            939..>1789
                     /locus_tag="PKL11_11750"
     CDS             939..>1789
                     /locus_tag="PKL11_11750"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNZ14318.1"
                     /translation="MNLIRKSELSRAEQIRQRRTQTSTPSEPKTPARPRPVTQTRTRA
                     ENPAPRRTSATPTRRQAAATPSRTRPVVARGGYGAPIVQQARTKARRQLYYNISATGV
                     EVRLPSLPIIRPDWRMLSGALAVALFVAIMLIWNAPSFQVGLIQVEGLQRVTADELNA
                     VLGLQGMSVLDLDVNELNQKLAVAFPELTNVRVNVSLPAQVTLSVTERQPILTWVYGD
                     KTLWVDGNGIIIPARGEISDIPVVIATDAPPILLESEAAVEGAQLEVLPAAFEEALLN
                     NWGRQVDP"
BASE COUNT          354 a          522 c          562 g          351 t
ORIGIN      
        1 caacagggcc gggccgacac attgacgcgc gtcacgctgc tgccccaacc cggcgatcac
       61 tggctgtatg ccattcagcc gggcggctgt ggtgaacaat tgaaccccct gacagaactg
      121 gacgtcattt ttccggtgct gcatggcagt tttggcgagg atggcaccct gcaagggctg
      181 ctggaaatgg ccaatatcgc ctatgtgggg gcaggtgtgc tgggctcggc gctgggcatg
      241 gataaagctg tgttcaaaga tgtgatgcga acctgcggtg tgcccgtgct gaattcggta
      301 accgtcaccc ggcagcaaat ccggcagggc ctgaacgatg tcatcgctgc cgctgagcag
      361 atgtcggctt acccgttgtt taccaaaccc gccaacctgg gttcttctgt gggcattacc
      421 cgctgccggg gccgtgccga tctggtggaa gggctgcaag aggccgcccg ctatgaccgg
      481 cggattctgg ttgagcaggg gctggatcac gcccgggaaa ttgaagtgag cgtgctgggc
      541 aacgaacagc cgcaggtttc ggttcccggg gaaatactgc cctgcgatga tttttacagc
      601 tacgaagcca aatatctgga tgaccgctcc cgcctggtca ttccggctga tttaccggcg
      661 gaggttacgg ctcaagttca ggcctacgcg ctcactgcct atcaagcggt tgactgcgcc
      721 ggtatggccc gggcagattt tctgctggag ccgaagacgc tggcaattta tttgaacgaa
      781 atcaatacca ttccggggtt cacccagatc agtatgtacc ccaagttgtg gcaggccagc
      841 ggcctgccct acccggcgct ggtggatcgc ttaattgaac tcgcgctgga acgcaaagcc
      901 gaaaacgacc tgaccgagcg cgaatacagg agggctgcat gaatcttatc cgtaaatccg
      961 aactgagccg ggctgagcaa attcgccagc ggcgaacgca aaccagtact cccagcgagc
     1021 ccaaaactcc ggcacgcccc cgcccggtga cgcagacgcg cacccgggct gaaaacccgg
     1081 caccgcgccg cacaagcgcc accccaacgc gacggcaggc agctgccacg ccgagccgca
     1141 cccggccggt ggtggcgcgc ggcggttacg gcgccccgat tgtgcagcag gcccgcacca
     1201 aggcccgccg gcagctctac tacaatatca gtgcgacggg cgtggaagtg cgcctgcctt
     1261 cactgccaat tattcgccct gactggcgga tgctttcggg cgcgctggct gtggccttgt
     1321 ttgtagctat tatgctgatc tggaatgcgc cgagcttcca ggttgggctt atccaggtgg
     1381 aagggcttca gcgggtcact gctgatgagc ttaatgccgt cctggggtta cagggcatgt
     1441 cggtgctgga cctggatgta aatgagctta atcaaaagct ggcggtggct ttcccagagc
     1501 tgaccaatgt gcgggtcaat gtgagcctgc cagcccaggt caccctgtcg gtcactgagc
     1561 ggcagcccat tctgacctgg gtctatggcg ataagacgct gtgggttgac gggaatggca
     1621 ttatcatccc ggcacgcggc gagatcagcg atattccggt ggtgattgcc accgacgccc
     1681 cgcccatttt gctggaatca gaagcagcgg tggaaggcgc tcagttggaa gtattgccag
     1741 ccgcttttga agaagcgctg ctgaacaact ggggccggca ggtggaccc
//
LOCUS       DAOPVS010000292         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_73810_length_1773_cov_47.380093, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000292 DAOPVS010000000
VERSION     DAOPVS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73810_length_1773_cov_47.380093"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKL11_11755"
     CDS             complement(<1..425)
                     /locus_tag="PKL11_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14319.1"
                     /translation="MSDQPPEPIQLPPPGFWQRIKLWFKQPAVLTTLRILALVVVVGL
                     SIWLVLNRERIESLAAFGYPGIFLVSALLNASLFLPLPTGVVISMMGAVFNPYLVAIA
                     AGSGSTLGEVSGYLAGFSGQPVMEKLDRQRKLEYWIKRYG"
     gene            complement(422..1066)
                     /gene="udk"
                     /locus_tag="PKL11_11760"
     CDS             complement(422..1066)
                     /gene="udk"
                     /locus_tag="PKL11_11760"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNZ14320.1"
                     /translation="MHTPESPLVIGIAGGSGSGKTTVANVILQRVGSHRIAYLAHDAY
                     YRELTGLPPVQRAQVNFDHPNSLENELLVQHIQQLKAWQPVEMPVYDFTCHARTGQTV
                     HVEPQRVILVEGILIFAERALRELFDVKIFVDTDADIRFIRRLQRDIAERGRTTEMVI
                     RQYYATVRPMHLEFVEPSKRYADVIIPEGGHNAVAMDMVIARIESLLAPGGIRS"
     gene            1199..>1773
                     /locus_tag="PKL11_11765"
     CDS             1199..>1773
                     /locus_tag="PKL11_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14321.1"
                     /translation="MLIYFGDPERGNVLRKPDTYLFQLIPTIHASGDGKFNPVGGSTG
                     GVTQMRPTLSKGLSVFLAAMFIAALAFGLSPAGAVEAATNSWVAQLPAGSPIYTQTVR
                     VWMNSDTVPGETAGVEIHNITAGTWTKFMGTYDNTSYPGANWYVDLPALAAGTQVEYQ
                     LFVRGTDSVDYGFSGFNWNYVVQNLPNTVYV"
BASE COUNT          440 a          446 c          529 g          358 t
ORIGIN      
        1 ccatagcgtt tgatccagta ttccagcttg cgctggcggt ccagtttttc catcacaggc
       61 tgaccgctga aacccgccag gtagcccgag acttcaccca gagtggagcc agagcccgcg
      121 gcaatggcga ccaggtaggg attaaaaacc gcgcccatca tggaaatgac cacaccggtg
      181 ggcaggggca aaaagagcga ggcattcagt aaggctgaga ccagaaaaat gccagggtaa
      241 ccaaatgcag ccagactctc aatgcgttca cggttaagca ccagccagat ggacagtccc
      301 accaccacaa ccaacgccag gatgcgcagg gtggtcagga cagcgggctg cttgaaccag
      361 agcttaatcc gctgccagaa gccaggcgga gggagttgga tgggctcggg gggttgatcg
      421 ctcatgagcg gatgccgccg ggggcgagca gcgattcaat ccgggcaatg accatatcca
      481 tggcaacagc gttatgaccg ccttcgggga tgatgacatc ggcatagcgc ttggatggtt
      541 cgacaaattc cagatgcatg gggcggacag tggcgtaata ttgcctaatg accatctcgg
      601 tggtgcggcc gcgctcagca atatcccgct gaaggcggcg aataaagcgg atgtcggcat
      661 cggtatcgac aaagattttg acatcaaaga gctcgcgtaa ggcacgctcg gcaaagatca
      721 aaataccttc aacaaggatg acccgctgtg gctcgacatg gaccgtctgc ccggtacggg
      781 catggcaggt gaaatcatag acgggcattt cgacaggctg ccaggctttg agctgttgaa
      841 tgtgctggac cagcagttcg ttttccagcg agttggggtg gtcaaagttg acctgggcgc
      901 gctgcacagg aggcaggccg gtcagttcac gatagtaggc atcatgggcg aggtaggcta
      961 tccgatggct gcccacccgc tggaggatga cattggcgac ggtggttttg cctgagccag
     1021 agccgccagc gataccgatg acaaggggag attcgggcgt atgcatatca aaattatagc
     1081 acgggatggg cactctaaaa attttaaggt aattccaaag taaatgtaag ttgaatatgg
     1141 attttcgccg tgctataatc actgtacagg caagtcgcaa gaagatcaac atcaggggtt
     1201 gctcatctac tttggagacc cagaaagggg aaatgtactc agaaagcctg acacatattt
     1261 gtttcagttg atcccgacca tccatgcaag tggggatggc aaatttaacc cggttggtgg
     1321 ttcaacagga ggagtgacac agatgagacc aacactctca aaagggctgt ccgttttttt
     1381 ggcagccatg tttattgcag cactggcatt cgggctctcc ccggctggtg cagttgaggc
     1441 ggcgacaaac agctgggtgg cccagcttcc cgctggcagt ccaatttaca cccagactgt
     1501 tcgcgtgtgg atgaacagcg atacggttcc gggtgaaact gccggcgtgg agatccacaa
     1561 catcaccgcc ggcacgtgga ccaaattcat gggcacctac gataacacca gttacccggg
     1621 tgccaactgg tatgtggacc ttcccgcgtt ggcggcaggc acacaagttg agtaccagct
     1681 gttcgtgcgc ggcactgaca gcgtggatta tggattcagc gggttcaact ggaactatgt
     1741 ggttcagaat ctgccgaaca ccgtttacgt cga
//
LOCUS       DAOPVS010000293         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_73812_length_1773_cov_40.480210, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000293 DAOPVS010000000
VERSION     DAOPVS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73812_length_1773_cov_40.480210"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKL11_11770"
     CDS             complement(<1..325)
                     /locus_tag="PKL11_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14322.1"
                     /translation="MSSVVIGILLAVILLVLLRRRGPAAGEAAIKPTPMRWGVFACGL
                     LALLTLAVAWAWPRVPAETVAARPFGVSVVFTVATVILGGVSLARRDRGWPTWAGLIA
                     GLIPAV"
     gene            complement(485..>1773)
                     /locus_tag="PKL11_11775"
     CDS             complement(485..>1773)
                     /locus_tag="PKL11_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14323.1"
                     /translation="AYGTIVTWLDANANGVMEPGELPLPGVKVWPLEMDMTFYGSTDR
                     NGEARIFKFRPGCCDHCWEDVDAVAEAPEGYIPTTPTSIELNGPDETYYFGFVADPDI
                     PTPTPYAPKLSCVTYPLEGTLHNLVTASDGTLWVGLSFSVAQFDSQQNQFVYYKSPKV
                     LDNLLLVDRDNTVWIRSLDGRLAALHDSEWTKFNNDSLLTASILSLGSTSDGTVWFVD
                     RLSQDIFVSFNPNSDEWRAYYDEHEQVFPGFQAVPFNNGTTWFAAFDYTERELSLTDE
                     SFTWEIAPRHVFSSDEQIEMPWLWPGDSLMDSNGTLWFSYSYHLINFVPNTGVWNTYD
                     IPDSSENYRDAGSAQNAMSPDQSIWFVTRVTYHSDNSPIIWRYFPDNGGSWLSYDDRD
                     IGLGDIEYDLIAFTGDGKLWLAEEYGQSVYSCEVLR"
BASE COUNT          440 a          493 c          468 g          372 t
ORIGIN      
        1 aaacggccgg gatcagcccg gcaatcagac cggcccaggt gggccagccg cggtcacggc
       61 gcgccaggct gacgccgccc aggatcaccg tggcaactgt aaagacgacc gaaaccccaa
      121 acggccgcgc ggcaacggtt tcagcgggaa cgcgcggcca ggcccaggca actgccaggg
      181 tgagcagcgc cagcaggccg caggcaaaga cgccccaacg catcggggtg ggtttgatgg
      241 ccgcctcacc ggcagccggc ccccggcggc gcaagagcac cagcagaatg accgccagta
      301 aaatccctat cacaacagat gacatcggac tgcctccttg aaaaataagg gccccaccgt
      361 tctgatggtt gcagcagtgc gtgctgcgtg gaactgtcat tgagcggaat tggggtttgg
      421 cccgggctat ataaaattat aaccccgcgt ccctcgcccg gtgacggtgg gcgtggcctg
      481 acttttacct caagacctca caggaatata ccgactggcc atactcttct gccagccaca
      541 atttcccatc gccggtaaat gcaatcagat catattcgat gtcgcccagg ccaatgtctc
      601 tgtcatcgta actcagccag gaccccccgt tgtctgggaa atagcgccag atgatagggg
      661 aattgtctga atggtacgtg accctggtga caaaccagat ggattgatcc gggctcatcg
      721 cattctgcgc cgagccagca tctctataat tctcacttga atcgggaata tcatatgtgt
      781 tccaaacgcc tgtattgggt acaaagttga tcaaatggta gctgtatgaa aaccatagtg
      841 tgccgttaga atccatcaag ctatcgccgg gccacaacca gggcatttca atctgttcgt
      901 cacttgaaaa gacatggcgc ggagcgattt cccaggtaaa ggattcgtcg gttaagctca
      961 gctctctttc agtgtaatca aaagcggcaa accaggttgt gccattgttg aacgggacag
     1021 cctgaaaacc cggaaacact tgctcatgct catcataata cgcacgccat tcatcagagt
     1081 tcggattgaa actgacaaag atatcttgtg acaaacgatc gacaaaccac acagtgccat
     1141 cagaggtgct gcccaggctt aaaatactcg cggtcaagag gctgtcgtta ttaaatttcg
     1201 tccattcaga atcgtgtaag gcggccaagc gtccatcaag cgatcttatc cagactgtat
     1261 tgtcgcggtc taccagcaga agattatcca gcaccttggg gcttttataa taaacgaact
     1321 ggttttgctg gctgtcaaac tgcgcaacac tgaaagacag gcccacccag agcgtcccat
     1381 ctgacgcagt caccaggtta tgaagtgtgc cttccagtgg ataagtgacg caggaaagct
     1441 tgggggcata gggggttggg gttggaatgt ctggatcagc cacaaagccg aagtaatagg
     1501 tttcatcggg gccatttaac tcgatgctgg ttggtgtggt ggggatatat ccttcgggcg
     1561 cttcagcaac cgcatcaacg tcttcccagc aatgatcaca gcagcccggt ctaaatttaa
     1621 atatccgggc ttcgccattt cgatctgtcg agccatagaa ggtcatatcc atctccaacg
     1681 gccagacttt aacccctggc aggggcagct cgcccggctc catcacccca ttggcgttgg
     1741 cgtcgagcca ggtaacgatc gtcccatacg cgc
//
LOCUS       DAOPVS010000294         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_76274_length_1735_cov_59.367857, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000294 DAOPVS010000000
VERSION     DAOPVS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76274_length_1735_cov_59.367857"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..760
                     /locus_tag="PKL11_11780"
     CDS             188..760
                     /locus_tag="PKL11_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNZ14324.1"
                     /translation="MSPASVITISRQMGSLGCEAASQAASALGFEVYQRELINQAARL
                     AGAPEAALAELDELGLLGLRPTPEETRAYLEAVRTVVTELAQRGRVIIIGRAGQTILQ
                     AFPGALHVRMVAPLEIRAQRIAEQKHVSLPAAMAQAQASDRNRKNYLRRNYQIRWDDP
                     ELYDLTLNTAHLTAEAAAEIICLAARQYIC"
     gene            784..1170
                     /locus_tag="PKL11_11785"
     CDS             784..1170
                     /locus_tag="PKL11_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14325.1"
                     /translation="MIAAQNTPPHFAHPAEETFARILDFYGIQWQYEPQTFPLEVDAE
                     GHILEAFTPDFYLPEQDLFIELTTLRPALATHKNRKLRRMAELYPQIRIKLLKRREMR
                     DLMIKFGLVDEAQSIQGTEAQVNGVK"
     gene            1170..1721
                     /gene="hpt"
                     /locus_tag="PKL11_11790"
     CDS             1170..1721
                     /gene="hpt"
                     /locus_tag="PKL11_11790"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNZ14326.1"
                     /translation="MMDLSGYGTLGEDIKEIVISEAQIQQRVKALGAQISQDYAEKNP
                     ILVGVLRGVVFFMADLLRAITIPVELDFLAVSRYKAQMGGVRLLKDLDFPIEGRHVLF
                     VEDLIDTGLTLNYLLRSLRERQPASLEVCALFSKTENRLIEIPLRYKGFDLPDRFVVG
                     YGLDHRERYRNLPFIGLLKPNAL"
BASE COUNT          388 a          415 c          532 g          400 t
ORIGIN      
        1 agcttgtttt gaccggccct aaattcatcc aaccgataca gttttgagtt ggcattgata
       61 tgggcccact catcccaaag cgagcgattg gcattgcgat attcatccat ggcggcctcg
      121 ttgtgtgtaa gttgaggcaa agtataacat cattcaatca gggggacatg ctataattaa
      181 tttgaccatg tcccctgctt cagtcatcac tatatcacgg caaatgggca gcctgggctg
      241 cgaagccgcc agccaggcag catcagcgct cggttttgag gtctatcaac gcgagttaat
      301 caaccaggcg gcgcggcttg ccggcgcgcc tgaggccgcg ctggcagaac ttgatgagtt
      361 gggtttgctg ggactgcgcc ccaccccgga agaaacccgg gcgtacctgg aagccgtgcg
      421 tacagtcgtc acggagttgg ctcagcgcgg gcgggtcatt attatcgggc gggcaggcca
      481 aacgatcctg caggcgtttc cgggggcgct gcatgtccgc atggtggcac cgctggaaat
      541 tcgggcacag cggattgcag aacagaagca tgtttcgctg ccggcggcaa tggctcaggc
      601 gcaggcaagc gaccgaaacc gcaaaaatta cctcaggcgg aattatcaaa tccgctggga
      661 tgaccccgag ctatacgatc tgacgctgaa cacagcgcac ctgaccgcgg aggcggcagc
      721 cgaaatcatc tgtctggctg cccgacaata tatctgttaa ttgcattaca gggagttgat
      781 tgcttgatcg ctgcacagaa caccccaccc cactttgcgc accctgctga agagactttt
      841 gcccgtattc tggattttta tgggatccaa tggcagtatg aaccgcagac atttccgctg
      901 gaagtggatg ccgaggggca tattcttgaa gcctttacgc cagattttta tctgccggag
      961 caggatttgt ttattgaatt gaccacgctg cggccagccc tggcaacgca taaaaaccgc
     1021 aagctgcggc gaatggccga actttacccc cagattcgca tcaagctgtt gaagcggcgg
     1081 gagatgcggg atttgatgat caagtttggg ctggtggatg aggcacagtc gattcagggg
     1141 accgaggccc aggttaatgg agtgaaatga tgatggatct ttcaggttac gggacgttgg
     1201 gagaggacat taaagaaatc gtcatctctg aggcgcaaat tcagcagcgg gtgaaggcgc
     1261 tgggggcgca gatttcgcaa gattatgcag agaagaaccc aattctggtg ggggtgctgc
     1321 ggggggtggt gttttttatg gcggatttgc tgcgggccat caccattccg gtggagctgg
     1381 attttttggc ggtttcgcgc tacaaggcac agatgggcgg ggtgcggtta ttgaaagacc
     1441 tggattttcc cattgagggg cggcatgtat tatttgtgga agacctgatt gatacgggat
     1501 tgacgttgaa ttatttgctg cgcagcctgc gagagcggca gccagccagc ctggaggtct
     1561 gtgcgctgtt cagtaaaaca gaaaaccggt tgattgaaat tccgttacgg tataaggggt
     1621 ttgacctgcc ggaccggttt gtggtggggt atggattgga tcatcgcgag cggtaccgca
     1681 acctgccttt catcgggctg ctgaaaccca atgccctgta aagaccggat tgggg
//
LOCUS       DAOPVS010000295         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_77122_length_1723_cov_56.562950, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000295 DAOPVS010000000
VERSION     DAOPVS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77122_length_1723_cov_56.562950"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKL11_11795"
     CDS             complement(<1..629)
                     /locus_tag="PKL11_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ14327.1"
                     /translation="MIWAEDLTKVFDDFTAVNSVHLDVQPGQVLVLLGPNGAGKTTTV
                     RMLTSILIPTRGQAKVAGYDVVTHPDKVRASVGVLTEHHGLYSRLSALEYLEFYARLY
                     GMDASIRLPRINNMLEQFGLAEARNRRCGQFSKGMRQKLALARALLHEPPVLLLDEPT
                     SAMDPESAFMVRNAIRSMRSTHRTIVLCTHNLTEAEDLADQIAIIQRGR"
     gene            complement(661..1680)
                     /gene="lgt"
                     /locus_tag="PKL11_11800"
     CDS             complement(661..1680)
                     /gene="lgt"
                     /locus_tag="PKL11_11800"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ14328.1"
                     /translation="MITADRYGISIAPDLLMGFLLGLFLIGIFFTVVLPYLQERKTNP
                     AAKLSIDWFSTGITLLVIVAGWFAFKKFGVDKVNIRFYGVIIMSGVVGAALLAAHRAP
                     RFGQNSDTVWDMIIWLLVGGVLGARLWHVLTPPPSMVEAGITTQWYFQHPLEMLMIWR
                     GGLGIVGGVAGGFLALFIYTRIKKLSLLSWTDIAAPGLLLAQAVGRWGNFINQELYGA
                     PTNLPWKLFIEPANRLPEYASVEYYHPLFLYESLWNLLSMGILFWLEKRFKHVFKPGD
                     SFFIYLILYPITRFTLEFLRLDASLLAGINANQWLMVIVIAFAAAMLIIRHRKSPAPA
                     EPSAV"
BASE COUNT          455 a          445 c          498 g          325 t
ORIGIN      
        1 atgcgcccgc gctggatgat ggcaatttga tctgccagat cttcggcttc ggtgaggtta
       61 tgcgtacata acacaatggt gcggtgggta gagcgcatgc tgcgaatggc attgcgcacc
      121 atgaaggcgc tttcggggtc catggctgag gtgggctcat ccaacagcaa gacagggggc
      181 tcgtgcagaa gcgcccgggc aagcgccaat ttttgacgca tgccttttga aaactgaccg
      241 cagcgccgat tgcgggcttc ggccaggcca aactgttcca gcatgttatt gatgcgggga
      301 agccggatgg aggcatccat gccgtacagc cgggcgtaaa attccagata ttccagagca
      361 ctgaggcgag agtagaggcc gtggtgttct gtgaggacgc caaccgaagc gcggactttg
      421 tcgggatggg tgaccacgtc atacccggcg actttggcct gaccgcgggt tggaatcagg
      481 attgaggtca acatgcggac ggtagtcgtc ttgccggcgc cattagggcc cagcaagacc
      541 agaacctggc cgggctgaac atccaaatgt acactattga cggcggtgaa atcatcaaac
      601 actttagtca aatcttcagc ccagatcata ttggcctcgc tttctctaaa aaacagtaaa
      661 tcagaccgcc gagggctcgg cgggggcagg tgatttgcgg tgccggataa tcaacatggc
      721 ggcggcaaaa gcaatcacga tcaccatgag ccactgattg gcgttaattc cggcaagcag
      781 cgacgcatcc agccggagga attccagcgt gaagcgcgtg atgggataca gaatcaagta
      841 aatgaagaag gagtcaccgg gcttgaaaac gtgtttaaag cgtttttcga gccagaacaa
      901 gatacccatt gagagtaaat tccacagcga ttcgtacagg aaaagcgggt ggtagtattc
      961 cactgaagcg tactcaggga gacgattcgc cggctcaatg aacagcttcc agggcaagtt
     1021 ggtgggggcg ccatagagtt cctgattaat gaaattgccc cagcgcccga ccgcctgcgc
     1081 cagcagcaga cccggagcgg cgatatccgt ccaggagagc aggctaagtt ttttgatgcg
     1141 ggtgtaaatg aacagggcca ggaagccgcc agcaacccca cccacaatgc ccaggccgcc
     1201 ccgccagatc atcagcattt ccaggggatg ttgaaagtac cactgcgtgg tgatgcccgc
     1261 ttcgaccatg gagggcggcg gggtgagcac atgccagaga cgggcgccca acaccccacc
     1321 gaccaggagc cagataatca tatcccagac ggtatcagag ttttgcccaa agcgcggggc
     1381 gcgatgtgca gccagcaagg cagcgccaac aacgcccgac atgatgatga caccatagaa
     1441 gcggatgttc actttatcga caccaaattt tttgaaggca aaccaaccag caacaataac
     1501 caacagggta atgccggttg aaaaccaatc aatgctcagt ttggcggcgg ggttggtttt
     1561 gcgctcctgc aaataaggca gcaccactgt gaaaaagatg ccgatcagga acaaacccag
     1621 cagaaacccc ataagaagat caggggcaat ggaaatgcca taacgatctg cagtaatcat
     1681 ctagtcagaa cctccaccca gattgggctt ctttgattat aag
//
LOCUS       DAOPVS010000296         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_77358_length_1720_cov_59.847447, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000296 DAOPVS010000000
VERSION     DAOPVS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77358_length_1720_cov_59.847447"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKL11_11805"
     CDS             <1..931
                     /locus_tag="PKL11_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ14329.1"
                     /translation="VNIFPIFESKPYFNDKIIIMQIREIQAKTILSTVPQPDPIFGLK
                     YNLNLYRGCQHQCIYCDSRSECYQIEDFNHEVLVKANALELLDQELRRKRVKGIIGTG
                     SMNDPYMPLEAERHLTRGALGLIARYGFGVHVLTKSTLVLRDLDLLQNISQAYAAVTF
                     TITTADDALSRQLEPGAPPSSERFAAIRQLAAQGLYTGVSLMPVLPFLEDTPENITAI
                     VHQAAEAGAQYIIPWISVTLRNRQRDYYYQQLDRLFPGLRQHYERAYGDAYHCEPPQA
                     HRLYTLLKEICAQVGLDTAIKPYQPPPAQPTLF"
     gene            983..>1720
                     /locus_tag="PKL11_11810"
     CDS             983..>1720
                     /locus_tag="PKL11_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14330.1"
                     /translation="MIAIVNGKVYTITHGTLEKGVVLIEGKKIVEVGENVTIPAGTQV
                     IDAAGKVVFPGLIDAHCHAGIFNDGVGWEHADGNETSDPITPHLRALDAVHPEDLAFP
                     DIISAGVTTILTGPGSANLIGGQWICIKTAPRASIEEMILQEPAGMKMALGENPRRVY
                     GSHDRAPSTRMANAGELRAALVRAQNYMQKWAQYETDLLAYEQKLAAGEADIKPPVPP
                     ERDLKMEALGKALRREMRVHIHAHRADD"
BASE COUNT          387 a          531 c          439 g          363 t
ORIGIN      
        1 tgtcaatatt ttccctattt tcgaatccaa accgtatttc aatgataaga taatcatcat
       61 gcagatccgt gaaatccagg ctaaaaccat tctgagcaca gtgcctcagc ccgaccccat
      121 ctttggtttg aagtataacc tcaacctgta tcggggctgc cagcatcagt gcatctactg
      181 cgattcgcgc agtgaatgtt accagatcga agattttaat cacgaagtgc tcgtcaaagc
      241 caatgctctg gaattgctgg atcaagagct gcgccgtaag cgggtgaaag gcatcattgg
      301 caccggctcg atgaatgacc cctatatgcc gttggaggct gagcgccatt tgacgcgggg
      361 cgcgctgggc ctgatagccc ggtatggttt tggcgttcac gttctgacga aatccaccct
      421 ggttctgcga gaccttgatc tgctgcaaaa catcagccag gcatatgccg ccgtcacctt
      481 caccatcacc accgcagacg atgccctctc ccgccagctt gaacccggcg ccccgcccag
      541 ttcggagcga tttgctgcca ttcggcagct ggccgcgcag gggctttaca ctggcgtttc
      601 gctgatgccc gtgctgccgt tcctggaaga tacccccgaa aatatcaccg ccatcgtcca
      661 ccaggcagca gaggctggcg cccagtacat catcccctgg atatccgtca ccctgcgcaa
      721 tcgtcagcgc gactactact atcaacagtt agaccgtctc tttcccggac tgcgccagca
      781 ctatgagcgc gcttatggcg atgcctatca ctgtgaaccg ccgcaagccc accgcctcta
      841 cacactgctg aaagaaatct gcgcccaggt tgggttggat accgccatca aaccttatca
      901 gccgccgcct gcccagccaa cacttttcta attttgtgat atactcggca ttagacaaat
      961 atctaatgac gaggagcttc gcatgattgc aattgtaaac ggcaaagtct acacaatcac
     1021 ccacggcact ctggaaaaag gcgtcgtgct gattgagggg aaaaagattg ttgaagttgg
     1081 cgaaaatgtg accatccctg ccggcactca ggtcattgat gccgctggca aagtggtctt
     1141 ccccggcttg attgatgccc actgccatgc tggcatcttc aatgacggtg ttggctggga
     1201 acatgccgat ggcaatgaaa cctccgatcc catcaccccg cacctgcgcg ccctggatgc
     1261 cgtgcatccc gaagacctgg cttttccgga tattatttcg gccggcgtca ccaccatcct
     1321 caccggccct ggctctgcca atctgatcgg cgggcagtgg atttgcatca agacggctcc
     1381 ccgtgcctca atagaagaaa tgatcctgca ggaaccggca ggcatgaaaa tggccctggg
     1441 cgagaacccg cgccgtgtct atggcagcca cgaccgggcg ccttccaccc gcatggcgaa
     1501 tgccggcgag ctgcgcgccg ccctggtgcg ggcgcagaat tacatgcaga aatgggccca
     1561 atacgaaacc gacctgctcg cttatgagca gaagcttgcc gctggcgaag cagatatcaa
     1621 accccctgtg ccgccggaac gcgacctcaa gatggaagcc ctgggcaagg ccctgcgtcg
     1681 tgagatgcgc gtccacattc acgcccaccg cgcggatgat
//
LOCUS       DAOPVS010000297         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_77568_length_1717_cov_41.794224, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000297 DAOPVS010000000
VERSION     DAOPVS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77568_length_1717_cov_41.794224"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKL11_11815"
     CDS             complement(<1..482)
                     /locus_tag="PKL11_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14331.1"
                     /translation="MTTPSGYLERLSKVKPIRVVAVYAVIVTLVAITLTRIPDSDPFL
                     ILLTAPILVSAAIFPRWVYLVEIGILIIATLGVTSHIVQNFSTSLITTSMVALATIIA
                     SEIILRFTTARRRLAESLQSEKRLFVGGPVVVFRWEPGDGLVASYVSPNIIDQWGYSA
                     "
     gene            703..>1717
                     /gene="hutU"
                     /locus_tag="PKL11_11820"
     CDS             703..>1717
                     /gene="hutU"
                     /locus_tag="PKL11_11820"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNZ14332.1"
                     /translation="MPGTRVVRAPRGTQLACKSWLTEAVYRMIQNNLDPEVAERPEDL
                     VVYGGRGQAARSWEAFDAILDTLRNLENDETLLVQSGKPVVVFKSHPDAPRVLIANSN
                     LVPHWATQSHFDDLAARGLIMYGQMTAGSWIYIGSQGIVQGTYETLASLAVQKGWNSL
                     AGKFVLTAGLGGMGGAQPLAITMNGGVGLCVEVDPARAHRRQEIGYVDLVVDTLEEAM
                     TLVEEACQQQKPLSIALIGNAAEIYPELVLRGVIPDVVTDQTPAHDVLFYVPAGLSVA
                     EADDLRRSDPQEYANRSMASMARHVEAMLEFQRRGAEVFDYGNNLRQRAFDYGVQNAF
                     DFPG"
BASE COUNT          394 a          494 c          433 g          396 t
ORIGIN      
        1 tccgccgaat atccccattg gtcaatgata ttgggtgaaa catagctggc aaccagccca
       61 tcccccggct cccagcggaa tacaaccact ggcccgccca caaacagccg cttttcagat
      121 tgcagcgatt ctgccaatcg ccgtcttgct gtcgtaaagc gcagaatgat ctccgaggca
      181 ataatcgttg ccagtgccac catcgaagtg gtgatcaacg aggttgaaaa attctgaaca
      241 atatgcgatg taacccccag agtcgcaatg attaaaattc caatttcaac cagataaacc
      301 caccgcggaa aaattgccgc gctcaccagg atgggcgccg tcaataaaat taaaaatggg
      361 tccgagtctg gtatccgtgt caatgtaatc gctacaagcg tcacaatgac ggcatacaca
      421 gccacaaccc ggatcggctt taccttgctt agtcgttcaa gataacctga tggagttgtc
      481 ataagtccag gattgcctga gaagaaaagc tgacatcatt atagcgtaaa atccgttctg
      541 gtgggctgtt caacccggcc gatttccagg taaatgtgcc cccggaaaaa taaccctcat
      601 atgaccattg ccgtgattaa ccgtgacatg cagttgataa gcaggttatg atttagcgta
      661 taattagccc agaacacttt acttattcag gaggaattgc tcatgcccgg tacccgtgtg
      721 gtgcgtgctc cgcgaggcac gcagcttgct tgtaaatcct ggttgactga ggcggtctat
      781 cgcatgattc agaacaacct ggaccccgaa gtggccgaac gccccgaaga cctggtggtt
      841 tatggcggcc gcggtcaggc tgcccgcagt tgggaagcct ttgatgccat tttggatacc
      901 ctgcgcaatc tggaaaatga tgagaccctc ctggttcaat cgggtaagcc ggtggttgtt
      961 ttcaaatcac accccgatgc cccccgcgtg ctgattgcca attctaacct tgtccctcac
     1021 tgggccaccc agtcccactt cgatgacctg gctgctcgcg gcctgatcat gtatggccaa
     1081 atgaccgccg gttcctggat ttatatcggc tcccagggca ttgtccaggg gacctatgag
     1141 accctggctt ctctggcagt tcaaaaaggc tggaactcac tggctggcaa atttgttctc
     1201 accgctggcc tgggcggcat gggcggcgcc cagccgcttg ccattaccat gaacggtggc
     1261 gttggcctgt gtgtagaagt tgaccccgcc cgcgcccacc gccgccagga aattggctac
     1321 gttgatctgg ttgtggacac gcttgaagaa gccatgacgc tggttgaaga agcctgccag
     1381 cagcaaaaac cgctgtctat tgcactgatc ggcaacgccg ccgaaatcta tcccgagttg
     1441 gtcctccgcg gcgtcatccc cgacgtagtc accgaccaga cgcccgctca cgatgtcctc
     1501 ttctatgttc ctgcaggcct cagcgttgcc gaagccgatg acctgcgccg ctctgatcct
     1561 caagaatatg ccaaccgttc aatggcctca atggctcgcc atgtcgaagc tatgctggag
     1621 ttccagcgcc gcggcgctga ggtgtttgat tacggcaata atctccgcca gcgcgccttt
     1681 gattatggcg ttcaaaatgc ctttgatttc cccggct
//
LOCUS       DAOPVS010000298         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_79126_length_1695_cov_47.982927, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000298 DAOPVS010000000
VERSION     DAOPVS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79126_length_1695_cov_47.982927"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKL11_11825"
     CDS             <1..797
                     /locus_tag="PKL11_11825"
                     /inference="COORDINATES: protein motif:HMM:NF021895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14333.1"
                     /translation="EARPIVVHVMETLAASHNLDDPKAKSEIAARVLPLIEDIPNAVE
                     RDDYRQRLARLLRVDERALTGSATTPATRPARRRPNLPGPRQTSSALADASPARLARS
                     LEAHCLRLLMRRPELVYKLDRMLQQGQLRRFSPEDFEQADFQTLARLLQEALGQDGEE
                     PQTYLQTHLPEDLEETAAALAAPITPNEPADDRMMEDLTRSLLKLRLVRISEGLQQLR
                     FLQEEMQQESGAAGLNPYQEIIMQYAQTRSRLDQALGKPQMGDQAA"
     gene            complement(794..>1695)
                     /locus_tag="PKL11_11830"
     CDS             complement(794..>1695)
                     /locus_tag="PKL11_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNZ14334.1"
                     /translation="DEESKTIIDSVTACLDGDMMAEMNADYGPGVESIAPGDVTDERY
                     FTIMDCLDDAHQYNPRAELFTYQQNANGELMVMVDAVATWDDNESYWLNDPYWIEDDI
                     EQEFMENGLVESDLGGMLFEEEDGNWYETFAPVKTSSGAPAGGIGLYMKANDLVERLE
                     ALNTWMILAFIVIYALVVALVFYITGRVTRRLITLSVASDRVAAGDYTPLNLPDLKLD
                     DEADRLAVAFNAMMDKVRVREETLQARVTQLEIQIDESRKAQQVSEIVDTEFFQDLAG
                     RAAAMRQRRQKPQDESGPTEPKA"
BASE COUNT          345 a          520 c          513 g          317 t
ORIGIN      
        1 ccgaggcgcg cccgattgtg gtgcatgtga tggaaacgct ggcagccagc cacaatctgg
       61 atgaccccaa ggccaagagc gagattgcag cccgggtgct gccactgatt gaagacatcc
      121 ccaacgcggt ggaacgggac gattaccggc agcggctggc ccgcctgttg cgtgtggatg
      181 agcgcgccct gaccggttcg gccaccacgc ctgccacccg cccagcccgc cgccggccca
      241 acctgcccgg gccccggcag acctcatccg ccctggctga tgccagcccc gcccggctgg
      301 cgcgctcgct ggaggcgcac tgcctgcggc tgctgatgcg ccgcccagag ctggtgtata
      361 agctggatcg gatgctgcag caggggcagc tgcggcgatt ctcacccgag gactttgaac
      421 aggccgattt tcaaaccctg gcccggctgc tgcaagaagc gctgggacaa gacggtgaag
      481 aaccccagac ttatctgcaa acgcacctgc ccgaagacct ggaagaaacc gccgccgcgc
      541 tggcggcgcc gattacgcca aacgagcctg ccgatgaccg catgatggaa gacctgaccc
      601 gcagtctgtt gaagctgcgg ctggtgcgca tcagcgaagg gctgcagcag ctgcgctttt
      661 tgcaggagga aatgcagcaa gagagcggag cggcagggct caacccctat caagagatca
      721 ttatgcagta tgcgcaaacc cgcagccgcc tggaccaggc gctgggcaag ccgcagatgg
      781 gcgatcaggc cgcttaagct ttgggctcgg tggggccgct ttcgtcctgg ggtttctgac
      841 ggcgctgacg catggcagcc gcgcggccag ccagatcctg gaagaattcg gtatccacaa
      901 tttcagaaac ctgctgagct ttgcgggact catcaatctg gatttccaac tgggtaacac
      961 gcgcttgcag ggtttcttca cgcacacgga ctttatccat catcgcgttg aaggccaccg
     1021 ccaggcggtc ggcctcgtca tccagcttga ggtcgggcag gttcaggggg gtgtaatcgc
     1081 cagcagccac acggtctgag gccacgctga gggtgatcaa ccgccgggtg acgcgcccgg
     1141 tgatgtagaa aaccagcgcc acaaccaggg catagatgac gataaacgcc aggatcatcc
     1201 aggtatttaa ggcctccagg cgttccacca ggtcgttggc cttcatgtag agaccaatac
     1261 cgcccgcagg ggcgccgctg gaggtcttga cgggggcgaa ggtctcatac cagttgccat
     1321 cttcttcttc aaacaacata ccacccaggt cagattcgac cagaccgttt tccatgaatt
     1381 cctgctcaat atcatcttca atccagtagg ggtcattgag ccagtaagat tcgttgtcgt
     1441 cccaggtggc cactgcatcc accatgacca tcagctcgcc gttggcgttt tgctgatagg
     1501 tgaacagctc ggcgcgcggg ttgtattgat gggcatcatc caggcagtcc atgatggtga
     1561 agtagcgttc atcggttaca tcaccgggag caatggactc caccccgggg ccgtaatcgg
     1621 cgttcatttc ggccatcatg tcgccatcca ggcaggcggt caccgaatcg atgatggtct
     1681 tggattcttc gtcca
//
LOCUS       DAOPVS010000299         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_81567_length_1663_cov_41.991294, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000299 DAOPVS010000000
VERSION     DAOPVS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81567_length_1663_cov_41.991294"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKL11_11835"
     CDS             complement(<1..526)
                     /locus_tag="PKL11_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ14335.1"
                     /translation="MRFFDLVSIIASNLSRRKGRVVLTAIGVVIGTAAVVLLVSLAIG
                     LQQSATSQLYGISDLRRIEVYPNYDTGGGGGGGGVVYSDKGQIPGSSEAKLLTPKALE
                     ELAALPGVEYVIPRDYLYYETHIVSGKLETWGQIIGIGTDNLANLGDTAETGVTTLER
                     GTAIIGGWAARNFYD"
     gene            complement(538..>1663)
                     /locus_tag="PKL11_11840"
     CDS             complement(538..>1663)
                     /locus_tag="PKL11_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14336.1"
                     /translation="DINFHQPMLADTDLWGRTLGVITMTLSYTNNDGTISYSENFVLT
                     IDIQTPNWVAATATPTISPLVPRPQIVVDSYASDVDPLQPGTIFNLTLNLRNLGNADA
                     RAVSMVLGGGVTLDASGTPQPGGVSGSGSDLSTFAPLGSSNVIYLGDLLAANTTSTTM
                     QLIVNVSANPGAYPLKLAFVYTDPKNNRLLDDQVITLLIYSLPQLDVNFYTDPGIFFS
                     NSMNMLPLQVTNLGRKSAVLGNITVTSENADISNNTALVGALEPGGYFTLDAQVMPYA
                     AGPLDLTVTINYTDDFNMVRTVTQTLTVNVEEMYIEPIPGENGEPPIIEPEPETFWDK
                     VLRFFKGLFGLDSAEPTPEPIPAETFPEDQPIEPVPGGKG"
BASE COUNT          332 a          489 c          480 g          362 t
ORIGIN      
        1 ggtcataaaa gttacgcgcc gcccagccgc caatgatggc ggtgccgcgc tccagcgtgg
       61 ttacccctgt ttcggccgta tcgcccaggt tcgccaggtt gtcggtgcca atcccaataa
      121 tctgccccca ggtttccagt tttcctgaaa caatgtgggt ttcataatat aggtagtcgc
      181 gagggatcac gtattccacc ccgggcaggg ccgccagttc ttccagcgct ttcggcgtca
      241 gcagcttggc ctcggatgaa cccgggattt gacccttatc gctgtagaca acgccgccgc
      301 ctccccctcc gccgccggtg tcatagttgg ggtaaacctc aatccggcgc aggtcgctga
      361 tgccgtaaag ctggctggtg gcgctttgct gcaggccaat tgccagcgag acgagcagca
      421 cgactgccgc cgtgccaatg acgactccaa tggccgtcag aaccacccgc cccttgcggc
      481 ggctcaggtt gctggcaatg atgctgacca ggtcaaagaa acgcatacgt gcagccttta
      541 tcctttgccg cccggaacgg gttcaatcgg ctgatcttcg gggaaggtct ctgctgggat
      601 gggttcggga gtcggctcgg cgctgtccag cccaaacagg cccttgaaga atcgcagcac
      661 cttatcccag aaagtttcgg gttccggctc aataatgggc ggctcgccat tttcaccggg
      721 gatcggttca atatacattt cttcaacatt taccgtcagc gtttgtgtca ccgtgcgaac
      781 catgttgaaa tcatccgtat agttgatggt caccgtcagg tcaagcgggc cagcggcata
      841 gggcatcacc tgggcatcca gcgtaaagta gccgccgggt tccagtgcgc cgaccagcgc
      901 ggtgttattg ctgatgtcag cgttctctga ggtcacagtg atgttgccca gcaccgcgct
      961 cttacgcccc aggttggtca cctgcaaggg cagcatattc attgaattgg agaagaaaat
     1021 tcccgggtct gtgtagaagt tcacatccaa ctggggcagc gaatagatca gcagcgtaat
     1081 cacctggtca tccagcaggc ggttgttctt cgggtcggtg tacacaaacg ccagtttgag
     1141 cgggtaggcc cccgggttgg cagatacatt aacaatcaac tgcatggtcg tgctggttgt
     1201 gtttgctgcc agcaggtcac ccagatagat tacgttggaa ctgcccagcg gcgcaaaagt
     1261 tgaaagatca ctgccgctgc ccgaaacccc gcccggctgc ggcgtgccag aagcatccag
     1321 cgtcacgccg ccgcccagaa ccatcgagac cgcccgcgca tcggcattgc ccaggttgcg
     1381 caggttcagc gtcaggttga agattgtgcc gggctgcagc gggtccacat cgctggcgta
     1441 gctgtccacc acaatctgcg ggcggggcac cagcggtgag atggtggggg tagccgtagc
     1501 tgccacccag ttgggcgtct gaatatcaat cgtcagcaca aaattctcgc tgtaactgat
     1561 tgtgccatcg ttattcgtgt agctcagcgt catggtaatc acgcccagcg tccggcccca
     1621 caggtccgta tctgccagca tcggctgatg gaagttgata tct
//
LOCUS       DAOPVS010000300         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_82459_length_1651_cov_58.828947, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000300 DAOPVS010000000
VERSION     DAOPVS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82459_length_1651_cov_58.828947"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..805)
                     /locus_tag="PKL11_11845"
     CDS             complement(68..805)
                     /locus_tag="PKL11_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ14337.1"
                     /translation="MSLVETQQLTKVYGQGETEVLALDHVNLKIEAGEFVAVMGPSGC
                     GKSTLLHLVGGLDRPTEGSVILNGHNLAALDDTRLTTVRRQQIGFIFQFFNLIPVLSA
                     QENVSLPLVLDGARAGTAAQKATDWLTRLGLEDQRHRRPDQLSGGQQQRVAIARALIS
                     EPALVLADEPTGNLDSRAGDEVAGLLRRVVDEWGRSILMVTHDPRIAAYADRIIFLKD
                     GRVIDETRLNAASVGEADRVKEKVEAL"
     gene            complement(827..1420)
                     /locus_tag="PKL11_11850"
     CDS             complement(827..1420)
                     /locus_tag="PKL11_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ14338.1"
                     /translation="MTVRHVILGLLAQQPRHGYELHAAFQAISGGQELWDVKPAQVYT
                     TLARLEESGLVVQEGTSQDAGPEKRIYALTETGQAELRTWLLSSIPSDAQRDAFYVKL
                     MLCLALGDVNPRTVIQVQRGSLYQELHRVTALRQSADPHTELARMLFLDKVVMHLEAD
                     LRWLDMIEARLDDVQQQPLPQPERRLRGRPPKQISTD"
BASE COUNT          341 a          444 c          510 g          356 t
ORIGIN      
        1 agggttgtga gggtggtgcg gagcttatgc cccgatagat aacgccaggc taacttccat
       61 gcgacgctca tagcgcctcc actttctctt tcacacgatc ggcttcgccc acactggcgg
      121 cgttcaaacg ggtttcatca atgacgcggc catccttgag gaagatgatg cgatcggcat
      181 aagcggcaat ccgggggtcg tgggtgacca tcaggatgct gcggccccac tcatctacca
      241 cccggcgcag caggccagcg acctcgtcgc cggcgcggga atccaggttg ccagtgggtt
      301 catcggccag gacaagggcg ggttcactga tcagggcacg ggcaatggca acccgctgct
      361 gctggccccc cgagagctgg tcggggcggc ggtggcgctg gtcttccaga cccaggcggg
      421 tcagccaatc ggtggctttt tgagcggcag tgccggccct ggcgccatcg agcaccaacg
      481 gcagagagac attctcttga gctgataaga ccggaatcag gttgaagaat tggaaaatga
      541 agccgatttg ctggcggcga acggtagtca ggcgggtgtc gtccagggcg gcgaggttgt
      601 ggccgttgag gattaccgag ccctctgtgg ggcggtcgag cccgcccacc agatggagca
      661 gagtagattt gccgcagccg ctcgggccca tcacggcgac aaattcaccg gcttcgattt
      721 ttagattcac atgatccagg gcgaggactt cggtttcacc ctgaccatag accttggtga
      781 gttgttgtgt ttctaccagc gacatattga tacctccatg aaaaaattaa tcggttgaga
      841 tttgcttcgg cgggcggccg cgcaagcggc gttcgggttg cggcaaaggc tgctgctgca
      901 catcgtccaa acgggcttca atcatgtcca accagcgcaa atcggcttcc aggtgcatga
      961 cgactttatc caaaaagagc attctggcca gctcggtgtg tgggtcggcg gactgacgga
     1021 gagcggttac ccggtgcagt tcttgataca ggctgccacg ctgtacctgg atgaccgtgc
     1081 ggggattgac atcacccagg gcgaggcaga gcatcagttt gacgtagaag gcgtcacgct
     1141 gggcatctga cgggatgctg ctgagcagcc aggtgcgcag ttcggcctga ccggtttcgg
     1201 tgagggcata gatgcgcttt tcggggccgg cgtcctggct ggtgccttcc tgaaccacca
     1261 gaccgctctc ttcgagacgg gcgagcgtgg tgtaaacctg ggcgggtttg acatcccaaa
     1321 gctcctggcc accagaaata gcctgaaaag cggcgtgcaa ttcgtagcca tgccgcggct
     1381 gctgcgctaa caaacccagg ataacgtgac ggactgtcat ataataaatc tattctacat
     1441 gatactagat ttatcatatc cctggatttg tcgtttgtca agagggttca ggtggaatcc
     1501 gaaactgttt ggtagaatta acaccgatga aacgaactgg aaatactccc ctgcccggag
     1561 aacgcctcaa attggtgcgg tttctgttgc atccaatcac tgtgctggca attggattgt
     1621 ttgccaccgc agcggtgctc atcttttggc g
//
LOCUS       DAOPVS010000301         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_83079_length_1643_cov_38.035264, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000301 DAOPVS010000000
VERSION     DAOPVS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83079_length_1643_cov_38.035264"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKL11_11855"
     CDS             <1..596
                     /locus_tag="PKL11_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ14339.1"
                     /translation="VRGLWVTFKHFITTYIEDLRRGPRFYKTPAGIEKRRRSDTTGIF
                     TVQYPEEKLPVPEEFRYLPFLVYDENADGSRAVRCTACGICSKVCPPQCISIVRSSDP
                     ATGKPVASPAEFYIDVDICMNCGFCAEFCPFDAIRMDHDYEVASVDRIAHHIFDLNRL
                     LKPASYYASIRPTNAAREDEARAAKAAAKKPGAETAA"
     gene            598..924
                     /locus_tag="PKL11_11860"
     CDS             598..924
                     /locus_tag="PKL11_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14340.1"
                     /translation="MYNEDTELIFPLRVVPSLRSLRSDSWSALVDQIASATADPLDQL
                     AFTLMMVRMASCASCGADSFRAMRGCTQCARQTVRRFRGSDADLLVQFQQYRIEVQHH
                     LQKQAA"
     gene            953..>1643
                     /gene="ricT"
                     /locus_tag="PKL11_11865"
     CDS             953..>1643
                     /gene="ricT"
                     /locus_tag="PKL11_11865"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNZ14341.1"
                     /translation="MTETEQPFVVGVRFCKVGKIYHFDAALIPDLKIGDPVVVETARG
                     WQLGEVASLVSDSNAPFEGGLKSVERRATPRDLLQRQLWQSREPEVLEAARQRARELH
                     LVGAKIVAVEFSFEGSRLSILYSTETEEKVDMKSLRQDMQRTFSPSQVELRQIGPRDA
                     AKIMGGMGACGLEMRCCTRFLTDFSSISIRMAKEQGISLTPPEITGMCGRLRCCLIYE
                     YQQYVDARQGLP"
BASE COUNT          354 a          495 c          419 g          375 t
ORIGIN      
        1 ttgtcagagg tctctgggtt accttcaaac actttatcac cacctatatt gaagacttgc
       61 gccgcggccc ccggttttat aaaacccctg ctggcattga aaaacgccga cgcagtgaca
      121 ccaccggcat cttcaccgtc caatatcccg aggaaaagct gcccgtcccc gaagaattcc
      181 gttatctacc cttccttgtg tatgatgaaa atgccgatgg ctcccgtgcc gtacgctgca
      241 cggcctgcgg catttgctca aaagtctgtc ccccgcagtg catttccatc gtccgcagtt
      301 cagatccggc caccggcaaa ccggttgctt ccccggccga attctatatc gatgtggata
      361 tctgcatgaa ctgcggcttc tgcgccgaat tctgtccctt tgatgccatc agaatggatc
      421 atgattacga ggtcgccagt gtggatcgga tagcccatca tatctttgat ctgaaccgcc
      481 tgctcaaacc ggcgtcatat tacgccagca ttcgacccac caacgcagcc cgtgaagatg
      541 aagcccgggc tgccaaagcc gccgccaaaa agcccggcgc agaaacagca gcctgaaatg
      601 tataacgaag ataccgaact catcttcccg cttcgcgttg ttcccagcct gcgcagtttg
      661 cgcagcgatt cctggtcagc cctggtggat cagattgctt cagccacagc cgatcccctc
      721 gatcaactgg ccttcacctt gatgatggtt cgcatggcaa gctgcgcctc gtgcggcgcc
      781 gattcgttcc gtgctatgcg cggatgcacc cagtgcgccc gccaaactgt tcgtcgattt
      841 cgcggcagcg atgctgattt actggtacaa ttccagcagt accggattga ggtacaacac
      901 cacttacaaa aacaggccgc ctgacggtct ttcttagaaa ggatttttat caatgaccga
      961 gacagaacaa ccatttgtgg ttggcgttcg gttttgcaag gttggcaaaa tctatcattt
     1021 tgacgctgct ctgattcccg acctgaagat cggtgacccc gtagtggtcg agacggcgcg
     1081 cggctggcag ttaggtgaag ttgcctctct ggttagcgat tcaaacgccc catttgaagg
     1141 tggtttgaaa agtgttgagc gccgcgccac cccgcgtgac ctgcttcaac ggcagctctg
     1201 gcaaagccgt gagcccgagg ttttggaggc cgcccgtcag cgggcccgtg aactccatct
     1261 tgtgggtgca aaaatcgttg ccgtagaatt cagctttgaa ggctcgcgat taagcatcct
     1321 ctacagcacc gaaaccgaag aaaaggtgga catgaagtcc ctccgccagg acatgcagcg
     1381 caccttcagc ccctcccagg tagaacttcg gcagatcggc ccccgtgatg ccgccaaaat
     1441 tatgggtggt atgggcgcct gcggcctcga aatgcgctgc tgcacccgct ttcttactga
     1501 tttcagctct atttccatcc gcatggcgaa agaacagggc atttcgctca ccccgccaga
     1561 gatcacgggc atgtgtgggc gcctgcgctg ctgcctcatc tacgaatacc agcagtatgt
     1621 ggatgcccgt cagggcttgc cca
//
LOCUS       DAOPVS010000302         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_83787_length_1634_cov_45.088664, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000302 DAOPVS010000000
VERSION     DAOPVS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83787_length_1634_cov_45.088664"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKL11_11870"
     CDS             <1..356
                     /locus_tag="PKL11_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNZ14342.1"
                     /translation="LSRDDFRLIDRQSRIYFRDIYDHLVRIQDMTESLRDIIGGDLDI
                     YLNSTSLRLNEVMKALTIVSTIFLPLSFVAGVYGMNFQHMPELSKTWGYPMAWGIFVL
                     IVVGMVTFFKKRKWF"
     gene            complement(353..1447)
                     /locus_tag="PKL11_11875"
     CDS             complement(353..1447)
                     /locus_tag="PKL11_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14343.1"
                     /translation="MGLPDLAPDANLLAVFESQGITSEWDQIALCLAALLDALSAANQ
                     PPTAETLSAAIPWAQSAAWPGGKVDYIARFSGLQPWLLPGIAPAESVLIACQRGEGAA
                     LFPALHQHPLLSDLLGHTRDFSQSWPQRVFQSYVLGNRIFEQVYGQSAPLNSRAYLGA
                     YDRSLLLKRFQERILAAQSSGSIHGAIYTARPSLLPSDLAATLPGYAPEAEMAQVIVG
                     LEPLPLVGYGGLRHLSERLGLHPDTLVKPSAAQALAAVGAALGAPVWQALRWAYALLA
                     RSEPALPPLPGDPPAEIRLPAALTIHIFEDSPIGILAGQRAAEILTGVGCTVKLRAWG
                     IASHPAKIKALESVGAAVCPDINAALSRIL"
BASE COUNT          359 a          415 c          557 g          303 t
ORIGIN      
        1 ggctgagccg ggatgatttc cggctgattg accgccagag ccggatctac ttccgagata
       61 tttacgatca cctggtacgg attcaggata tgaccgagtc gctgcgggat atcattggcg
      121 gcgacctgga tatttacctc aattccacgt ctctgcgatt gaacgaagta atgaaggccc
      181 tgaccatcgt ctcgaccatt ttcctgccgc tctcttttgt ggcgggcgtg tatggtatga
      241 attttcaaca catgccagag ctctcaaaaa cctggggcta tcccatggca tgggggattt
      301 ttgtgctgat tgtggtgggg atggtgacgt ttttcaaaaa gcgcaagtgg ttttagagaa
      361 tacgggagag ggcggcgtta atatcgggac aaaccgccgc accgaccgat tcaagcgctt
      421 tgatctttgc cgggtggctg gcaatgcccc aggcgcgtaa cttgaccgtg caccccaccc
      481 cggtcagaat ttcggctgcg cgctgaccgg ccaaaatgcc aatgggggaa tcttcgaaga
      541 tgtggatggt gagcgcggcc ggaaggcgga tctcggcggg cgggtcaccg gggagcgggg
      601 gcagtgcagg ctctgaccga gccagcagag cataggccca gcgcaaagcc tgccagaccg
      661 gcgcacccag ggctgcgcca acagccgcca gcgcctgagc ggcagagggc ttgaccaggg
      721 tatcgggatg caggccgagg cgctcgctga gatggcgtaa gccgccgtaa cccaccaacg
      781 ggaggggctc caggccgacg atgacctggg ccatttcagc ttcgggggca tagccgggca
      841 gggtggcagc caggtcagaa ggcagcaggg aggggcgggc ggtgtagatg gccccgtgaa
      901 tactgcccga ggattgagcc gcgaggattc tttcctgaaa ccgtttgagc agcaggctgc
      961 gatcataagc gccgagatag gcgcgggaat tcaatggcgc agactggcca tacacttgct
     1021 caaaaatacg attgcccaac acgtaagact ggaaaacacg ctgaggccag gattggctaa
     1081 agtcgcgggt gtggcctaat agatctgaca gaagggggtg ttggtgcaag gcggggaaga
     1141 gcgctgcgcc ttcgccacgc tgacaggcga tcagaacgga ttcagcgggg gcaattccgg
     1201 gcagcagcca gggctgcagg cctgaaaacc gggcgatata atccaccttg ccgccgggcc
     1261 aggctgccga ttgcgcccag ggaatggcgg cagacagcgt ctcggctgtg ggtggttgat
     1321 tggcggcgga aagcgcatcc agcaaggcag ccagacagag cgcaatttgg tcccactcgc
     1381 tggtgatacc ctgtgactca aagaccgcca gaagattggc gtcgggggcc aggtcgggca
     1441 ggcccatctg gtggcagaag aatcgaactg tatcaaaaac ggcctgctga tagccgcccg
     1501 gttggatgag gacgccatcc aggtcaaaga gggccagggt tgttgagggg ggcagggttg
     1561 ttgagggggg cagggggtgg cgtgatgcct cagattaaaa tgttacttta gaagtattgt
     1621 tgcctcaaat aaga
//
LOCUS       DAOPVS010000303         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_84444_length_1626_cov_40.284532, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000303 DAOPVS010000000
VERSION     DAOPVS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84444_length_1626_cov_40.284532"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..671
                     /locus_tag="PKL11_11880"
     CDS             144..671
                     /locus_tag="PKL11_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14344.1"
                     /translation="MKQNFVLQFDRKEIPDWAEKYSTLTDLEKKIEAEIAPGARARGY
                     YTREDFLDLCYWKTPRTKPLVRSNTTETIQELTRLALSKPHERLRIETLTYLRGVGWP
                     TASVLLHFGYDNLYPILDFRALWSLGLEKPPSQYDFDFWMDYTHYCRQLATEVNVSMR
                     TLDRALWEYSKQNQK"
     gene            697..1026
                     /locus_tag="PKL11_11885"
     CDS             697..1026
                     /locus_tag="PKL11_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14345.1"
                     /translation="MKIHVEIYIAARLLAQNHPTFSPQDLINFIQREFGDERPGVRTH
                     ATAVCIANAPLHHPNGCNYLWRVGHGEMRLFQPSQDLPHPDRVNAPTQPQRADMPAKY
                     LHLLRPE"
     gene            1101..>1626
                     /locus_tag="PKL11_11890"
     CDS             1101..>1626
                     /locus_tag="PKL11_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14346.1"
                     /translation="MGDIHTGDFSNINNSIINIGSGNVEVHIPELKLIPHQLPDPPAD
                     FVGRAAELDELCAAVQTQGALICGLTGLGGVGKTALGLVLASRLKAHYPDGQLYIHLR
                     GASNNPVTPAAALEQLIRAFEPVARLPEDVDQLAAIYRTLLTGKHILVFLDDARDAAQ
                     VRALLPPRPALAIVT"
BASE COUNT          367 a          496 c          415 g          348 t
ORIGIN      
        1 ccgcgcctat cgtccctggc gcctggatga tatcctgcgg ccttaccggt aaccgacctg
       61 aaccaggttt ctccattgca taactgaatc cttacccgct gtataataaa gtcagaaaat
      121 gatggatcaa aaggtggtgt gttatgaagc aaaactttgt gctgcaattt gaccgaaaag
      181 aaattccgga ctgggcagaa aagtattcaa ccttgaccga cctggagaaa aagattgaag
      241 ccgaaattgc tcccggagcg cgtgcgcgcg gctattacac ccgggaagat tttttggacc
      301 tgtgctattg gaagacgccg cgcaccaaac cgctggtacg aagcaacacg accgaaacga
      361 tccaggaact cacacggttg gcgctttcaa agccccacga gcgtctgcgc attgaaacac
      421 tgacctatct ccggggtgtc ggctggccca cagcctcggt tctgttgcac tttgggtatg
      481 acaacctcta ccccatcctg gattttcgcg ccctctggtc gctgggattg gaaaagccgc
      541 cttcccagta cgactttgat ttttggatgg actataccca ctactgccgg cagttggcga
      601 ccgaggtcaa cgtctccatg cgcaccctgg accgtgcgtt gtgggagtat tcaaaacaaa
      661 atcaaaagtg acctaccgga ggaggaacca agaataatga aaatccatgt tgaaatctat
      721 atcgctgccc gcctcctggc gcaaaaccac cccaccttct cgccgcaaga tttgatcaac
      781 ttcatccagc gtgaatttgg cgatgaacga cctggtgtcc gcacacacgc aactgctgtt
      841 tgtattgcta atgctcctct ccaccatcca aacggttgta actatctttg gcgcgttggg
      901 cacggcgaaa tgcgcctttt ccaacccagt caagatctgc cacatccaga ccgcgttaat
      961 gcccccacac agccgcagcg tgctgatatg cctgccaaat atttgcacct cctgcggcca
     1021 gagtaagcaa ctttctataa caatgccgaa tggtatgcgt aaccgagctt ttatgacccg
     1081 acaccggtat aattaacgcc atgggcgata ttcacacggg cgatttctcc aatatcaaca
     1141 acagcatcat caacattggc tcgggcaatg ttgaagttca catccccgaa ctgaaattga
     1201 ttccgcatca gctccccgac ccgccggcgg actttgtggg ccgggccgcc gaactggacg
     1261 agctctgcgc cgcggtgcag acccagggcg cactgatctg cgggctgacc ggcctgggcg
     1321 gggttggcaa gaccgccctc ggtctggtgc tggcaagccg cctgaaggcc cactaccccg
     1381 acgggcagct ttacatccat ttgcgcgggg cttccaacaa tcccgtgacg cccgctgcgg
     1441 cgctggagca gttgatccgc gcctttgaac ccgtggcccg cctgcccgaa gacgtagacc
     1501 agcttgccgc catttaccgc accctgctga cgggcaagca tatcctggtc tttctggatg
     1561 atgcccgcga tgcggcccag gtgcgggccc tgctgccgcc ccgcccggcg ctggcgattg
     1621 tgacca
//
LOCUS       DAOPVS010000304         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_85608_length_1612_cov_63.676301, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000304 DAOPVS010000000
VERSION     DAOPVS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85608_length_1612_cov_63.676301"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKL11_11895"
     CDS             complement(<1..185)
                     /locus_tag="PKL11_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNZ14347.1"
                     /translation="MSKRIPETTKNPRERAFLVSAAVRSEPGLLSLEDSLAELRLLSD
                     TAGIDVVGEATQNLEKP"
     gene            complement(194..1387)
                     /locus_tag="PKL11_11900"
     CDS             complement(194..1387)
                     /locus_tag="PKL11_11900"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNZ14348.1"
                     /translation="MTTQTSFIRPADRIASFKPYFFASLVQKINGLKASGMDVIRIDM
                     GSPDLPPADFIVDALEKSARKANTHGYSPNGGTPAFREAIANYYKTRFNVDLDPKSET
                     LQLIGSKEGLFNLSEVLLNPGDVSLVPDPGYPVYSASGIIAGAEVVYLPLNRETGFLP
                     DLDAIPEETARRAKILWLNYPNNPTGAIAPMAFLEKAVAFAKKYEIVLAHDAPYTDVC
                     FDGYRAPSILEIPGAKDVAVEFNSLSKTYNMAGWRLGMAVGNAQVINYLHTFKSQVDS
                     SQFQPILDAGIAALAGDQSWLEERNEIYQHRRDVVLQGLRAAGFTVDTPPAAIYVWAQ
                     LPAGEKDSITFCNRLLEETGVSTTPGIVYGQHGEGYLRISLGTATHRIEEAMERITRW
                     VKAKK"
     gene            complement(1412..>1612)
                     /locus_tag="PKL11_11905"
     CDS             complement(1412..>1612)
                     /locus_tag="PKL11_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ14349.1"
                     /translation="KFKGGGPGLGLHICKGIVEAHGGAIWVESPGCDEHHCPGSTFHV
                     LLPLLSEPPDDKLARLFADHKL"
BASE COUNT          351 a          448 c          416 g          397 t
ORIGIN      
        1 tggggcttct ccaggttttg ggttgcctcg ccaaccacat caattccagc cgtgtctgag
       61 agcagacgta gttcagccag agaatcttca agcgagagca acccgggttc gctgcgaacg
      121 gctgcactga caagaaacgc ccgttcgcgc ggatttttag ttgtttcagg tattcgtttt
      181 gacatttatc ctatcatttc ttggctttta cccaccgggt aatgcgttcc atcgcttctt
      241 caatgcgatg ggtcgcggta cccagtgata tgcgcagata accttcaccg tgttggccat
      301 aaacaatccc cggtgtcgtg ctgacgccgg tttcttccaa caaacggttg cagaaagtaa
      361 tgctgtcctt ttcaccagcc ggcaattgcg cccagacgta aatggccgct ggcggcgtat
      421 ccacggtgaa tccagccgcc cgcaggccct gaagcacaac atcgcgccgg tgctggtaga
      481 tttcgttgcg ttcttccagc cagctctgat cgcccgccag ggctgcaatc cccgcatcca
      541 aaatgggctg gaattgcgag ctgtccacct ggcttttaaa ggtatgtaga taattaatca
      601 cctgtgcgtt acctactgcc atgcccaatc gccaccccgc catgttatag gtcttggaca
      661 gcgagttgaa ttcaaccgcc acgtctttgg ctcccggaat ctccaggatg ctgggcgccc
      721 ggtaaccgtc aaagcagaca tcggtgtacg gtgcgtcatg tgccagcaca atttcatact
      781 tcttggcaaa cgctactgct ttttccaaaa acgccattgg cgcaatagct ccggttggat
      841 tgttggggta attgagccac aaaatcttgg cgcgccgggc cgtttcttcg gggatggcgt
      901 ccagatctgg cagaaagccc gtctcgcggt tcaatggcag ataaaccacc tcggccccgg
      961 caataattcc gctggctgaa tacactggat agccagggtc aggaaccagc gaaacgtctc
     1021 ccggattcag cagcacttcg ctcaagttga acaggccttc cttagaaccg atcaattgca
     1081 gcgtttcact tttggggtcc aggtcaacgt tgaagcgtgt cttgtagtag ttggcgatcg
     1141 cttcccgaaa agcgggcgtg ccaccgttag gcgaatatcc atgcgtgttg gccttgcggg
     1201 cagatttctc cagggcatcc acaatgaaat ccgccggcgg cagatcgggc gaacccatgt
     1261 caatccggat gacatccatt ccgctggctt ttaacccgtt aattttttgc accagtgatg
     1321 caaaaaagta gggtttgaac gaggcgattc gatctgccgg acgaataaat gaggtttgag
     1381 tggtcatggg tctccctttt aatgatttga tttagagttt atggtcggca aagagccgcg
     1441 ccagtttatc atctggcggc tcacttaaca atggcaaaag cacgtggaag gttgagcccg
     1501 ggcagtgatg ctcatcgcag cccggcgatt ctacccagat tgctcctcca tgcgcttcta
     1561 caataccttt gcaaatatgc agccccagcc ccggaccgcc gcccttgaat tt
//
LOCUS       DAOPVS010000305         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_85944_length_1608_cov_62.808113, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000305 DAOPVS010000000
VERSION     DAOPVS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85944_length_1608_cov_62.808113"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..1355
                     /locus_tag="PKL11_11910"
     CDS             384..1355
                     /locus_tag="PKL11_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNZ14350.1"
                     /translation="MSLSSISNQINHALGAVTWSAEEIADFQQHTGAASVKKSLRPAG
                     DPTRPLPVILGVGTRTAYFQSAVCFFQRAEALSGRGLLVDLLTPEIIQQTLDAFYQAQ
                     APGSLAKLVAAIGKVYAGCRKLGWARQPNPITPQLREYIKGIADGPGVRSPRFGYQFT
                     DAERIVAYLTERSSRFALPAELALRCGLREDEIAGLRGQDIDLARSCLHVTGKGGKVR
                     TVPMPTDIAARLNPSMQYQFTPSSAWRSAFRQTVARAAKALGIQLTGVHRLRANFAQQ
                     RYLRLRQDGLDDQAARLEISHLLGHNRLDVTYHYIPDGFSEEDAAKE"
BASE COUNT          344 a          458 c          444 g          362 t
ORIGIN      
        1 gaattcaaaa ctcggtacag catggactct gtgaatgatt ttgcggattg acccgtatta
       61 atcgactcga tatcataaag ataagctcga cgcgcttgcg gcgtcaaaaa aattagtcgg
      121 ctgcggtcaa tttagaaacc aatttttttt aaatttttat ccttgctctg tcggttgttc
      181 gtgccgccct ccctggcggt gacccgtaga gcccagtccg gaggcgctta cccaattatt
      241 ggggtgggag gccggcagga agccagcgcc aacgattgca aaattctcct gcggtacgcc
      301 aggcacctgg cgaacggctc aacagcaacc atgcttaacc tagaaacgtc tggaaaccca
      361 gtttgcagcg aacggagaac tgtatgtcgt tatcttccat ctcgaaccaa atcaatcacg
      421 cgctcggggc agtcacctgg agcgctgaag agattgctga tttccagcag cacacaggag
      481 cggcctcggt taaaaaatca ctgcgcccag cgggtgaccc aacccgcccg ctgccggtca
      541 tcctgggtgt gggcacccgc acggcttact ttcaatcggc agtctgcttt tttcaacgcg
      601 ctgaggcttt gtctggccgg ggcttgctgg tcgatctgct gacccctgaa atcatccaac
      661 aaacgctgga cgcattttac caggcgcagg cgcctggttc gctggcaaag ctggtagcgg
      721 caatcggcaa agtttatgcc ggctgccgca agctgggctg ggcacgccaa cccaacccca
      781 tcaccccaca acttcgagaa tacatcaagg gcattgctga tgggccgggg gttcgctccc
      841 cccggtttgg ctatcagttt actgatgccg agcgcattgt tgcctacctg accgagcgca
      901 gctcgcggtt tgcactgccg gctgagctgg cactgcgctg cggtctgcgt gaagacgaga
      961 ttgccggcct gcgcggtcag gacattgatc tggctcgcag ctgcctgcac gtcaccggca
     1021 aaggcggcaa ggttcgcaca gtgcccatgc cgacggatat cgccgcccgg ctcaatcctt
     1081 cgatgcagta tcaattcacc cccagctcag cctggcgctc tgcttttcgt cagacagtgg
     1141 cgcgggccgc caaagccctg ggcattcagc ttacaggcgt tcaccgcctg cgcgccaatt
     1201 ttgcccagca gcgctatttg cgtctgcgcc aggatggcct ggatgatcag gccgcccgcc
     1261 tggaaatttc tcacctgctg ggacataacc gcctggatgt cacctatcat tacattccgg
     1321 acggattcag cgaggaggac gccgcgaaag agtaatcttg ttggtctgag tttgttcaat
     1381 ccggattccc ggaaatgtcc ctaatgccaa cggcacaggg aaaggagtaa aaatggataa
     1441 gcataatatt gatctgatgg acgagacttc atgctgggct tgtcacacga tttgcatcat
     1501 tgaagggctg gatttgccgg aatcgcgcgt ggcagacgag ttgcgcctgg tttatgatta
     1561 cgcttcgatt gaatatgaaa attgccctgg cagcgccttc tggcagcg
//
LOCUS       DAOPVS010000306         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_86718_length_1599_cov_59.873705, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000306 DAOPVS010000000
VERSION     DAOPVS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86718_length_1599_cov_59.873705"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1599
                     /locus_tag="PKL11_11915"
     CDS             <1..>1599
                     /locus_tag="PKL11_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ14351.1"
                     /translation="VKEANLQLGGSPLQIRIGLHSGMAFLGAVGAAGEYTVMGDTVNT
                     ASRLQHIAPVGGVILSNETYQLVRGIFDVQPLDPVRLEGKTEPMMAYLALRAKQRAFR
                     MSTRGLEGIRTRMIGRAAELFILQNALQSVMTASRHQVITVIGDAGIGKSRLLYEFEN
                     WLELQSMPIRYFKGRASPETQRLPYGLLRNMFAFRFQINETDSPAAARQKFEQGVAAV
                     LSTLDPEQAAIRAHFIGQLIGFDFSASPYLQGVDAQQLRNRAYLYLTEFVRAAAQLTM
                     VLFLEDIHWADDSSLNLLTTLLSADFDRSLLVVCLARPTLYERYADWGAGLPRHTPLR
                     LNALSRDDSRLLVTEILQKAGQVPEVLREMVVGGAEGNPFYVEELIKMLIEDGVIVKG
                     EEEWQIDATRLVQVRVPGTLAGVLQARLDSLGPAERHCLQQASVIGRVFWDDALAHLA
                     GEGIDLTTALQTLVQRELIFQRPVSVFAGAHEYTFKHALLREVTYESVLKRDRRIYHA
                     RAAEWLLAHGGERSREYTGLVAQHL"
BASE COUNT          292 a          524 c          462 g          321 t
ORIGIN      
        1 ggtcaaagaa gccaacctgc agttgggcgg cagcccgctg caaatccgca ttggcctgca
       61 ctctggcatg gctttcctgg gtgcggtcgg cgccgcgggg gaatataccg tcatgggcga
      121 tactgtcaat acagccagcc gcctgcagca cattgccccg gttggcggcg tgattttatc
      181 caacgaaacc tatcaactgg tgcgcggtat atttgatgtc caacccctgg acccggtgcg
      241 cctggaaggt aaaaccgaac ccatgatggc ctacctggcg ctgcgtgcca aacagcgggc
      301 ctttcgcatg agcacccgtg gtttggaggg catccgcacc cgcatgattg gccgcgccgc
      361 cgaacttttc attttgcaga atgcgctcca gtccgtaatg acggccagcc gtcatcaggt
      421 tatcacggtc attggcgatg ccggcattgg taaatcccgc ctgctctatg agtttgaaaa
      481 ctggctggaa ctgcaatcca tgcccattcg ctactttaag ggtcgtgcca gccctgaaac
      541 ccagcgtctg ccctacggcc tgctgcgcaa catgtttgcg tttcgcttcc aaatcaatga
      601 aaccgattcg cctgccgccg cacgccagaa gtttgagcag ggggtggctg ctgttctcag
      661 caccctggat cccgaacagg ctgccattcg ggcgcatttc atcggccaat tgatcggctt
      721 tgatttcagc gccagcccct atctgcaggg cgtagatgcc cagcagctgc gcaaccgcgc
      781 ctacctttac ctgacggagt ttgtgcgcgc cgcggcccaa ctgaccatgg tgctgttcct
      841 cgaagacatc cactgggccg atgacagctc cctgaacctg ctcaccacgc tcctgagcgc
      901 cgattttgac cgctcgctgc tggtggtctg cctggcccgc cccaccctct acgagcgcta
      961 tgccgattgg ggcgccgggc tgccccgcca tacgcccctc cgcctgaatg ccctctcacg
     1021 ggatgacagc cgcctgctgg tgaccgagat cctccagaaa gccggtcagg tgcccgaggt
     1081 tttgcgtgaa atggtggtcg gcggggccga aggcaacccc ttctacgtcg aagaactgat
     1141 caaaatgctg attgaagatg gcgtcatcgt caaaggcgaa gaagaatggc agattgacgc
     1201 cacccgtctg gttcaggtgc gcgtccccgg cactctggca ggcgttttgc aggcccgcct
     1261 cgacagcctg ggcccggctg agcgccactg cctgcagcag gcctcggtaa tcggccgcgt
     1321 cttctgggat gatgccctgg cgcacctggc cggcgaaggc attgatctga ccactgccct
     1381 gcaaaccctg gtgcagcgtg agctgatctt ccagcgcccg gtcagtgtct ttgcgggcgc
     1441 ccacgaatac accttcaaac acgccctgct gcgtgaggtc acctatgaaa gcgtcctcaa
     1501 gcgcgaccgc cgcatctatc acgcccgcgc ggccgaatgg ctgctggccc atggcggcga
     1561 gcgcagccgg gagtacaccg gcctggttgc ccagcacct
//
LOCUS       DAOPVS010000307         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_88004_length_1584_cov_59.246566, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000307 DAOPVS010000000
VERSION     DAOPVS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88004_length_1584_cov_59.246566"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PKL11_11920"
     CDS             <1..1040
                     /locus_tag="PKL11_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ14352.1"
                     /translation="ITFLFVGPAINILAITYTGATIGMDIAISRLVLSIAFGIIIGLI
                     MAWIFRKEETAREAEMTASGVFDQTARVKPAVWVLFLLLVAVLIVGTLQVGLFTKVYA
                     SIHLPFDPASGFVAFLSTLNLTVQGALLILMLVVIGITAWQGFEKIFDGFNAWTYIAF
                     AMIALTILIAAPKEDIGSLVIGINGRFIGELVLLAAIGVVAGRSFTRDELAGWIFETW
                     KFVKQIFPLLIVGVFLAGIVKVIIPEAWVRTIAGQNTLWANLVGVIFGVFMYFPTLVE
                     VPVAKMFLDLGMARGPLLAYLLADPELSLQSILVLNGVMGKKKTAIYVGLVALFSTLA
                     GYLFGLAIAGL"
     gene            1094..1477
                     /locus_tag="PKL11_11925"
     CDS             1094..1477
                     /locus_tag="PKL11_11925"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HNZ14353.1"
                     /translation="MDCCSTSCSSSNTPTHNPEIGKTAERLAALLKQTAEYQEFVRLA
                     SLVNQDPDVRRILLEIRQQQMMYSDPAGTSVEALQAELETLPAVQAYRTAEAAVKTLF
                     QTVDRAIGAAAGVEFAPNALRSGCG"
BASE COUNT          315 a          425 c          454 g          390 t
ORIGIN      
        1 cgatcacctt cctgtttgtg ggcccggcga tcaacattct ggccatcacc tacaccggcg
       61 caaccatcgg gatggatatt gccatctcgc gcctggtctt atccatcgcc tttggcatca
      121 tcattggtct gatcatggcc tggatcttca gaaaagagga aaccgcgcgc gaggctgaaa
      181 tgacggccag cggcgtattt gatcaaacag ccagggtgaa gccagccgtg tgggtgctgt
      241 ttcttttact ggtggcggta ttgattgtcg gcaccctgca ggtgggtctg ttcacaaagg
      301 tatatgccag catccatttg ccctttgacc cggcaagcgg cttcgttgcc ttcttatcaa
      361 cgctgaactt aaccgttcag ggcgctctgc tgattctgat gctggtggtc attggcatta
      421 ccgcctggca aggctttgag aagatttttg acggcttcaa tgcctggaca tatatcgcct
      481 ttgcaatgat tgcgctgacg atcttgattg ccgcaccgaa agaggacatc ggctcgctgg
      541 tgattggcat caacgggcgc ttcatcggtg agctggtttt gctggctgcc attggggttg
      601 tggctggccg atcattcacc cgggatgaac tggcgggctg gatttttgag acctggaagt
      661 tcgtcaagca aatcttcccg ctgctgattg tgggcgtctt tcttgccgga atcgtcaagg
      721 tcatcattcc cgaagcctgg gtgcgtacca ttgccgggca aaacaccctc tgggccaacc
      781 tggtcggtgt gatctttggg gtgtttatgt actttcccac cctggtggaa gtgccggtgg
      841 ctaaaatgtt cctggacctg ggcatggccc gcggcccgct gctggcttac ctgctggctg
      901 accccgaact atcgctgcaa tccattctgg tgctgaacgg ggtgatgggg aagaagaaaa
      961 ccgccattta tgtcgggctg gtggctctat tcagcaccct cgccggatac ctgtttggcc
     1021 tggcgattgc agggttataa tggggctggc gcaagccgcc cgtagatcat cattgactga
     1081 aaggattttg cgaatggatt gttgttctac atcttgctct tcatcaaata cacccaccca
     1141 taacccggaa attggaaaaa ccgccgaacg cctggctgcg ctgctcaagc agactgctga
     1201 atatcaggag tttgtgcgcc tggccagcct ggtcaaccag gaccccgatg tccggcgtat
     1261 tttattggag atccgccagc aacaaatgat gtattcggac ccggcgggga cctctgttga
     1321 ggccttgcag gccgagctgg agaccctgcc cgccgtgcag gcctaccgca ctgccgaggc
     1381 tgccgttaaa acgctgttcc agacggtaga ccgggcaatt ggcgcggctg ccggggtaga
     1441 atttgccccc aatgcactgc gcagcggctg tggttgaggg ggctgaaatt gggtcaggtc
     1501 tgacccactc accctctaac caccgaatga atcaggagta catcatcatg gaattaatgg
     1561 ctgagattac acaaaccgct caag
//
LOCUS       DAOPVS010000308         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_88721_length_1576_cov_40.466798, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000308 DAOPVS010000000
VERSION     DAOPVS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88721_length_1576_cov_40.466798"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKL11_11930"
     CDS             complement(<1..229)
                     /locus_tag="PKL11_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ14354.1"
                     /translation="MDIAIQAESLTKIYPNQRGLPALRDLDLTIPAGILFGLVGPDGA
                     GKTTALRILSSVMLPTSGAVHVLGYDTRRQPD"
     gene            388..>1576
                     /locus_tag="PKL11_11935"
     CDS             388..>1576
                     /locus_tag="PKL11_11935"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNZ14355.1"
                     /translation="MMNRPIEPGLLKIFKYFTSIAMAYFAILWAYAILYAGWEWSLQL
                     QLWLNFAVYLGLAGYLAWGELEQRLKQFYLPIGLAISTLFPVLNNFVYLMGPYQANID
                     IIISRSWLWLPVLLVPLVLIAWQYNLKSVIGFTVFTNGLELIVLLVVVKTINFDTIAL
                     LGVPLVRAFAFGIVGYVIGNLMETQRNQQQKLVQANVRLGQYASTLDQLATSRERNRL
                     ASELHDTLAHTLSGLAITLEAIKTVLDPQQTEATEMLEHSLQATRVGLDETRRALKAL
                     RARPLEDMGLKLALEKVSQAAAERAAIPIDFIYPAELPALPTDVEQCMYRVTQEALEN
                     MVRHASATQAAVEVRSNEGQIELIIRDNGIGFEPSEKKLEDRFGLFGLHERAKSVAGQ
                     LEVH"
BASE COUNT          359 a          372 c          475 g          370 t
ORIGIN      
        1 gatctggctg ccgacgggta tcatagccca acacatgaac tgcgccgctg gtgggaagca
       61 tgaccgatga aagaatgcgc agggcggtgg tcttaccggc gccatcgggg ccaaccagcc
      121 caaacaagat gcccgccggg atggtcagat caaggtcacg cagggcgggc agcccgcgtt
      181 gattggggta gattttggtc aaagattcag cttgtatagc gatatccatg cggttatctc
      241 ctgcgatatt gctctatcac tataaggcat tgcgataacc gtggcgagat gcaagacagc
      301 atgaaacggt atgacgggcg ccacgcgttg gaatgatggg cgtcatcaac ttgtatgagg
      361 tcgttttaaa tcgtgttatg ataaatcatg atgaaccgcc cgattgaacc cggactgctg
      421 aaaatattca agtatttcac cagcatagca atggcttatt ttgccattct gtgggcatat
      481 gcgattcttt atgccgggtg ggagtggtcg ctgcagcttc aactctggtt gaattttgcg
      541 gtttatctgg ggctggcggg ttatctggcg tggggcgagc ttgagcaacg gctcaagcag
      601 ttttatctgc ccattgggct ggcgatcagc acgctttttc cggtgttaaa caattttgtt
      661 tatctcatgg ggccctatca ggccaacatt gatatcatca tcagccgcag ttggctgtgg
      721 ctgccggtgc tgctggtgcc gctggtatta attgcctggc aatataacct caagtcggta
      781 attgggttca cggttttcac caatgggttg gaattgattg tgctgttggt ggtggttaaa
      841 accatcaatt ttgatacgat tgcactgctg ggggtgcccc tggtgcgcgc ctttgctttt
      901 ggaattgtgg gctacgtgat tggaaacttg atggagaccc agcgcaatca acagcaaaaa
      961 ctggttcaag ccaatgtacg gttggggcaa tatgccagca cattagatca actagcgacc
     1021 agccgggaac gcaaccggct ggcaagcgaa ctgcatgata cgctggcgca tactttgagc
     1081 ggtttggcga ttaccctgga ggcgatcaaa acagttctgg acccccagca gaccgaagcg
     1141 accgagatgc tggaacactc gctgcaggcc acccgggtgg ggctggatga aacccgccgc
     1201 gccttgaaag ccctgcgggc gcgcccgctt gaagatatgg ggctgaaact ggcgctggaa
     1261 aaggtcagtc aggcggcggc agaacgggca gccatcccga ttgattttat ctacccggca
     1321 gagctgccgg ccctgccaac tgatgtggaa cagtgcatgt atcgggtgac gcaggaagcg
     1381 cttgaaaata tggtccggca tgccagcgcc acgcaggctg ccgttgaagt gcggagcaat
     1441 gaggggcaga ttgagctcat catccgggat aacggcattg ggtttgaacc gtctgaaaaa
     1501 aagcttgaag accggtttgg attatttggc ctgcacgagc gggcaaaatc tgttgccggg
     1561 cagcttgaag ttcaca
//
LOCUS       DAOPVS010000309         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_89504_length_1567_cov_64.229497, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000309 DAOPVS010000000
VERSION     DAOPVS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89504_length_1567_cov_64.229497"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1338)
                     /locus_tag="PKL11_11940"
     CDS             complement(10..1338)
                     /locus_tag="PKL11_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNZ14356.1"
                     /translation="MTEDMELSKRELDILRLVATGASNKEIAQALFISPNTVKVHLRN
                     IFGKIGAASRTEATLYAVRSGLVEGAPVQPAPEVDEAEEALPIPEPEVVVVIPPKPTF
                     WRRPGILIAAAVVLLAVLGVGGWQIWKATRPTPTPEITSAPLTGITVQPRWQTAESIP
                     TASSGQASAIYEQDVYLIAGQTEAGVTGEVWAYQLREKTWTPRTAKPTPVSGAQAALL
                     SEHIYVPGGLDVSGKPTAVLEVYDPRLDSWEALAPMPRALSGYALAGLEGRLYLFGGW
                     DGEKFSTDVYVYDPDNNQWETRQPLPEARSAASAVAAGNKIFLVGGTNGEKLLTDLLV
                     YFPTREENGEDPWEVRTSLPAGRSNAGAALIADGIYLVGGGEPPLLYMIAEDQWTELE
                     APPQPLGAGVTVQVWENLLHVFGGSVEARPSDQHFTYQALYTLTLPVINR"
     gene            1493..1564
                     /locus_tag="PKL11_11945"
     tRNA            1493..1564
                     /locus_tag="PKL11_11945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1525..1527,aa:Ile,seq:cat)
BASE COUNT          324 a          478 c          415 g          350 t
ORIGIN      
        1 ggttgttttt tatcggttaa tcactggcag cgtcagggtg taaagcgcct ggtaggtgaa
       61 atgctggtcg ctgggtcggg cttccacaga accgccaaac acatgcagca ggttttccca
      121 gacctgcacc gttacacctg cgcccaatgg ctgcgggggc gcttccaact ctgtccattg
      181 gtcctcggca atcatgtaca gcaggggtgg ttcaccgccg ccaaccaggt aaatgccatc
      241 ggcaatcagc gccgcgcccg cattggatcg gcctgccggc aggctggtcc gcacttccca
      301 cggatcttcc ccattttcct ctcgggttgg gaagtagacc agtaaatccg tcagcagttt
      361 ttccccattg gtgccaccaa ccagaaagat cttgtttccg gcggcaacag cgctggctgc
      421 gctgcgggct tctggcagtg gctggcgggt ttcccattga ttattatccg ggtcataaac
      481 atatacatcg gtgctgaact tttcaccatc ccagccgccg aacaggtaca ggcggccttc
      541 cagccctgcc agtgcatatc cgctcagtgc cctgggcatc ggcgccagtg cttcccagct
      601 gtccaaacgg ggatcataga cttccagcac ggccgtaggc tttccgctga catccagccc
      661 tcccggcaca taaatatgtt cgctgagcag cgccgcctga gcgccgctca caggggttgg
      721 tttggcagtt cggggcgtcc aggtcttctc gcgcagttgg taggcccaaa cctcaccggt
      781 gaccccggct tcagtttgcc cggcaatcaa atacacatcc tgttcgtaaa tagccgaagc
      841 ctgcccgctg ctggcagtag ggatagattc agcagtttgc cagcgtggct gcacagtgat
      901 gccggtcagg ggggcgctgg taatctcagg tgtcggggta gggcgggtgg ccttccagat
      961 ctgccagccg ccaaccccca gcacagccag cagcacaact gccgcagcga tgagtattcc
     1021 aggtcgcctc cagaaagttg gcttcggtgg aatgaccacc actacttctg gctccggtat
     1081 gggcagagcc tcttcggctt catctacttc aggtgccggc tgaactggcg ccccctcaac
     1141 cagaccgctg cgcaccgcgt aaagggtggc ctctgtgcgt gaagccgccc caattttccc
     1201 gaaaatattg cgcaaatgca ccttgaccgt attcgggctg ataaacaacg cctgagcaat
     1261 ctccttattg ctcgcccccg ttgccaccag gcgtagaata tccaattctc gtttgctgag
     1321 ttccatgtcc tcggtcatag cgctccaatt ataacccagc ccagcctcca gcgtatgata
     1381 taatagcgcg agattagagg taagtgataa cttgaagatc acaggcctga taatttgccc
     1441 gtttcttcat atcataaatc tcaaatctca aatctaaaat caaaaattgc aggggcctgt
     1501 agctcagtgg cagagcgcgc ggctcataac cgcttggtcg atggttcgaa cccatccagg
     1561 cccacaa
//
LOCUS       DAOPVS010000310         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_90181_length_1560_cov_66.828571, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000310 DAOPVS010000000
VERSION     DAOPVS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90181_length_1560_cov_66.828571"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..441
                     /locus_tag="PKL11_11950"
     CDS             64..441
                     /locus_tag="PKL11_11950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNZ14357.1"
                     /translation="MSDQRKTERKKLIKFTPVYEYQKNRVLGYLGDLTLQGAMMLGEK
                     PMEVNQTLTLVIEFHETPETPATRVVQPARVAWCRQEKDSSFFNTGFEFIDLTADNQM
                     IIEAILARYQFRREPLDLFDEPR"
     gene            471..983
                     /locus_tag="PKL11_11955"
     CDS             471..983
                     /locus_tag="PKL11_11955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNZ14358.1"
                     /translation="MPQPALLVIDVQRGLFQKSTPIYHAETLLDNILALVSRAQAAGA
                     PVIYVQHCAERDLAKGTDAWQLHPRLAPRPTDPLVFKEHGNAFEATPLQEILTARRVD
                     RLVACGLVTHGCVKATCLGALALGYPVTLAADAHSSYSQKADQLIKDWNAKLQAAGAV
                     VRPTTEIVFG"
     gene            1077..>1560
                     /locus_tag="PKL11_11960"
     CDS             1077..>1560
                     /locus_tag="PKL11_11960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNZ14359.1"
                     /translation="MIDSALLVIDVQYGLFHESTPIYKAKTLLDNVLVLISRAQAAGV
                     PVIYVQHCEEEGLVKDTEAWQLHPRLAPRPTDPLVFKEYGNAFKATPLQEILTARGVK
                     RLVLCGLMTPYCVQSTCLGALALGYPVTLAADAHSTNGEQVAKVIKEWNTKLQAAGAV
                     V"
BASE COUNT          346 a          452 c          453 g          309 t
ORIGIN      
        1 gcggatattc atacaatgat tatgacaatc ggtgacaccc gccctgaggt ttggaggccg
       61 cgcatgagtg atcaacggaa gactgaacgt aaaaagctga tcaaatttac accggtttac
      121 gagtaccaga aaaaccgggt gctgggatac ctgggagacc tgaccctgca aggggcgatg
      181 atgctcggcg agaagccgat ggaggtcaac cagacgctga cgctggtgat tgagtttcac
      241 gaaacgcccg agacgcccgc cacgcgggtg gttcaaccgg cgcgggtggc ctggtgccgc
      301 caggagaaag actcttcttt cttcaacacc ggctttgaat ttattgacct gaccgccgac
      361 aatcagatga ttatcgaggc cattctggct cgctaccagt tccggcgcga gccgctggat
      421 ctgtttgacg aaccgcgtta aaccaatact cttcggaaag tgagccatcc atgccgcagc
      481 cggctttgct ggtcattgac gttcagcgcg gattgttcca gaaatccacg cccatctatc
      541 atgccgaaac cctgctcgat aatatcctcg ccctggtcag ccgggcgcag gctgccggcg
      601 cgccggtgat ctatgtgcag cactgcgccg agcgtgacct ggcgaaaggc accgacgcct
      661 ggcagttaca cccccggctg gcgccgcgtc ccaccgaccc gctggtgttc aaggaacacg
      721 gcaacgcctt tgaagccacc cccttgcagg aaattctgac cgcccgcagg gtggatcggc
      781 tggtggcctg cggcctggtg actcatggct gcgtcaaagc cacctgcctg ggcgcgctgg
      841 cgctgggcta tccggtcacg ctggcggcag atgcacacag cagctacagc caaaaggccg
      901 atcagttgat taaggattgg aacgccaagc tccaggccgc gggggcagtt gtcaggccga
      961 cgaccgaaat cgtgtttggg taaatgcccc gatatgagtt gtgattttca acggcatcag
     1021 ggatataatc cagccagacc gcgcaaaacc cggatatttt tctacatcga ggtaacatga
     1081 ttgattctgc attgctggtc attgacgttc agtacggact gtttcacgaa tccacgccca
     1141 tctataaagc caaaaccctg ctcgacaatg tccttgtcct gatcagccgg gcgcaggctg
     1201 ccggtgtgcc ggtgatttat gtgcagcatt gtgaagagga aggcctggta aaagatacgg
     1261 aagcctggca attacacccc cggctggcgc cgcgtcccac cgacccgctg gtattcaaag
     1321 aatatggcaa cgcctttaaa gccacccccc tgcaggaaat tctgaccgct cgcggggtga
     1381 agcggctggt gttgtgcggc ctgatgaccc cgtactgcgt tcagtccacc tgcctgggtg
     1441 cgctggcgct gggctatccg gtcacactgg cggcagacgc gcacagtacc aatggcgaac
     1501 aggttgccaa agtgatcaaa gaatggaaca ccaaactgca ggctgccggg gcagtcgtca
//
LOCUS       DAOPVS010000311         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_91613_length_1544_cov_41.953660, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000311 DAOPVS010000000
VERSION     DAOPVS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91613_length_1544_cov_41.953660"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKL11_11965"
     CDS             complement(<1..482)
                     /locus_tag="PKL11_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ14360.1"
                     /translation="MNIAILVQWLATFAWIGLVGFFIYLLVRAAQHRPIPRGGVGILI
                     IGIVAVLLTTVASGLVFIKPDERGVVISAVAPGGYRPLPLEPGLRWIIPFAETVERYP
                     ISRQTYTMSIAPSEGSIQGDDSIEARTADGQKIYVDASVIYSINPAEVVNVHILWNDR
                     "
     gene            complement(820..1173)
                     /locus_tag="PKL11_11970"
     CDS             complement(820..1173)
                     /locus_tag="PKL11_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14361.1"
                     /translation="MNNETSESPLLRYLKSTRFLTNGIIAWAVILALLGGIFIQYGLK
                     KPLPDLYLDVVTFGVAVLGVFASLVIIIRKEMPLRHGLASIRGWLAQVAGVLGLLIWG
                     GVAYYGLTQILGALP"
     gene            complement(1213..>1544)
                     /locus_tag="PKL11_11975"
     CDS             complement(1213..>1544)
                     /locus_tag="PKL11_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14362.1"
                     /translation="EKVKQTTSTPEYLTGRFLDLGVLLAFAMGGLWSLITRSRVPDLY
                     LNLSALFLGGLGVWTNIYVMIYQQARVRHRTIHGQAARLLGGLGILLWLGVGSWGMAS
                     LLEPLLH"
BASE COUNT          388 a          451 c          429 g          276 t
ORIGIN      
        1 tagcggtcgt tccacaggat atgcacattg accacctctg ccgggttgat ggaatagatg
       61 accgaggcat ctacatagat tttttgccca tcggcagtcc gggcctcaat tgagtcgtcg
      121 ccctggattg aaccctcaga aggtgcaatg gacatggtgt aagtctggcg ggagatgggg
      181 tagcgctcca cggtttccgc aaatgggatg atccagcgca ggccaggctc cagtggcagg
      241 gggcggtagc cgcccggcgc caccgccgaa atgaccacgc cgcgctcatc gggtttgatg
      301 aagaccagcc ccgaggccac cgtggtgagc agcaccgcaa cgatgccaat aatcaaaatg
      361 cccaccccac cgcgcgggat ggggcggtgc tgcgccgcgc gcaccagcag ataaataaaa
      421 aagcccacca ggccaatcca ggcgaaggtg gctaaccatt gaaccaagat cgcaatattc
      481 atgaggttgc tcctgaaaac ggatgaaaga atcaaacagg attaatatac cataaatggg
      541 gggcaggatt tggaatttta gattttagat ttgagatttg ggattatggc agtggggtaa
      601 gagagtttga gccgcattca acgccgcctg acccaaaacc aagcccgcta aagcagactc
      661 atccagacga agtcggcgca gccgacttga tctttccata gccggggatt tcaatccccg
      721 gaggtttggc ccgggcacgg gcccctaccc ggaactgacc gaatcaaccg gcaggccaaa
      781 cggactgccg cccgtggaac ctggaacgaa tataaacccc tacggcagtg cgccgaggat
      841 ttgtgtcaga ccgtaataag ccaccccacc ccagatgagc agccccagca cacccgccac
      901 ctgagccagc cagcctcgga tgctggccaa gccgtgacgc agcggcattt ctttgcggat
      961 gatgattacc aggctggcaa acacccccag aactgccacg ccaaaagtca ccacgtccag
     1021 atacaggtcg ggcagcggct ttttgagccc atattgaata aagatcccac ccagcagcgc
     1081 cagaatcaca gcccaggcaa tgatgccgtt ggtcaggaaa cgggtgcttt tgagatagcg
     1141 cagcagtgga ctttcggagg tttcgttgtt cataatcggg tatcctttgg atgcaagttc
     1201 aacagcctga ttttaatgca gaagcggctc gagaagcgaa gccatccccc aactgcccac
     1261 gcccagccag agcagaatgc ccagcccgcc cagcaaccgg gccgcctgcc cgtggatggt
     1321 tctatggcgc acgcgcgcct gttgatatat catgacataa atattggtcc acacacccag
     1381 cccgcccaga aagagcgccg acaggttcag gtacagatcg ggaacccgac tgcgggtgat
     1441 gagcgaccac aacccgccca ttgcaaaagc cagcagcaca cccaggtcca ggaagcggcc
     1501 tgtaaggtat tccggggtgg aggtcgtctg cttgaccttt tcaa
//
LOCUS       DAOPVS010000312         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_91868_length_1541_cov_37.726110, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000312 DAOPVS010000000
VERSION     DAOPVS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91868_length_1541_cov_37.726110"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /gene="hypE"
                     /locus_tag="PKL11_11980"
     CDS             complement(<1..1072)
                     /gene="hypE"
                     /locus_tag="PKL11_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNZ14363.1"
                     /translation="MTQLPNFEGPVCPMPLQHNETIVIGHGSGGRMTHDLIQKVFLPR
                     LSGGQPGSGNDFAGAGLPAPAGLNGRLAVSTDSHIVKPLFFPGGDIGRLAVCGTVNDV
                     SMSGATPLYLTAGFILEEGLPMATLEKVLISMQDAAAEAGVQIIAGDTKVVEKGAADG
                     LFINTAGIGWIPAGQSIGGEMARPGDVVLISGNLGDHGIAVLAARGELGFETDVQSDV
                     APLNGLIQTVLEAAPQVHVLRDPTRGGLATTLNEIAHQSNVNITIEEKRIPVQPAVRA
                     ASEMLGFDPLYIANEGKLIVIVPAEQAEAALAAMRGHPYGRNAVRIGEVKAAPERRVL
                     LHTLIGGHRILDMLAGEMLPRIC"
     gene            complement(1081..>1541)
                     /locus_tag="PKL11_11985"
     CDS             complement(1081..>1541)
                     /locus_tag="PKL11_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNZ14364.1"
                     /translation="YHIPIVVTGFEPLDLVQGILMTVQQLEKGTCEVQNAYPRLVTEE
                     GNRAAQAIIQKVFEPCDRNWRGIGMIPMSGWGLRPELSDFDAEQRFEVAGIQTQESPL
                     CMAGLVLQGLKKPSECPAFGTLCTPQNPLGAPMVSSEGACAAYFRYHRPD"
BASE COUNT          327 a          493 c          379 g          342 t
ORIGIN      
        1 agcaaatcct gggcagcatt tcacctgcca gcatatccag tatccggtgc ccgccaatca
       61 gcgtatgcag cagcacccgc cgctctggcg ctgccttgac ttctccaatc cgcaccgcat
      121 tccgcccata cgggtgcccc cgcatggctg ccagcgccgc ttcggcttgc tcagccggta
      181 caatgacaat caatttacct tcattcgcaa tgtagagtgg gtcaaatccc agcatctcgc
      241 tggctgctcg cacagcaggt tgaaccggga tgcgcttctc ctcaattgta atgtttacat
      301 tgctctggtg tgcaatttcg ttcagtgtgg ttgccagtcc gccgcgagtg gggtctctca
      361 aaacatgaac ctgcggcgct gcctccagca cggtttgaat cagcccattc agcggcgcca
      421 catccgactg cacgtcggtt tcaaagccaa gttcaccccg cgccgccaac acggcaatgc
      481 cgtgatctcc caggttcccg gagattaaca ccacatcgcc cgggcgggcc atttcacccc
      541 caatggattg tcccgcgggt atccagccaa tgcctgcagt gttgataaac aaaccgtctg
      601 cggcgccttt ttcaaccacc ttggtatccc cggcgatgat ttgtaccccg gcttctgctg
      661 ccgcatcctg cattgaaatc agcacttttt ccagggttgc catgggcagc ccctcttcaa
      721 gaataaaccc ggctgtcagg tagagcgggg tggcgccaga catgctcaca tcgttgacgg
      781 ttccgcagac cgccagccgt ccaatgtcac caccagggaa gaacaacggt ttcacaatat
      841 gcgaatctgt cgaaactgcc agccgcccat tcagccctgc cggtgctggt aagcctgctc
      901 cagcaaaatc attgccagat cccggctgtc ccccggacaa tcgcggtaaa aagactttct
      961 gaatcaagtc atgggtcata cgcccgccgc tgccatgtcc gatcacaatt gtctcattgt
     1021 gctgtaatgg cattgggcag accgggcctt caaaattggg taattgggtc attgcgctcc
     1081 tcaatccggg cgatgatagc ggaaataagc tgcacaggcg ccttctgatg agaccatcgg
     1141 cgctcccagc ggattttgcg gggtacacag tgtcccaaat gccgggcatt ctgagggctt
     1201 ctttaaccct tgcagcacca gacccgccat acacagcggc gactcttgcg tttgaatccc
     1261 cgcgacctca aagcgctgct cagcatcaaa atcagatagc tctggacgca gcccccagcc
     1321 gctcatgggg atcattccaa taccgcgcca gttgcggtca cacggctcaa atactttttg
     1381 aatgatcgcc tgtgccgccc ggttgccttc ttcggtaacc agccgcgggt aagcattttg
     1441 aacctcacag gtgccttttt caagctgctg cacggtcatc aaaatgccct gaaccaggtc
     1501 cagcggctca aatccggtaa ccacaatcgg aatgtggtac t
//
LOCUS       DAOPVS010000313         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_92904_length_1530_cov_39.785085, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000313 DAOPVS010000000
VERSION     DAOPVS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92904_length_1530_cov_39.785085"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PKL11_11990"
     CDS             <1..1409
                     /locus_tag="PKL11_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ14365.1"
                     /translation="GRFHGPVSVRSALANSFNIPAVKALEYIGIYDNPATEWPDGLVP
                     FAQRLGITTLTRNDYGLALTLGGGDVSLLEMTGAYAVFANGGQQVPPVAITRIVDHEG
                     NVVYEYEPPEPVQALRSEHAYLISSILSDNAARTPMFGANSVLNLPFAAAAKTGTTND
                     YRDNWTIGYTPDLVTGVWVGNADYTPMQNTTGLTGAAPIWAQFMQFAVPQLTGGYPTP
                     FTRPPGVVDLVVCSVSGAQPSQWCPEQRTEIFAADQPPAPADQDLWQKVLVDTWTGLR
                     ASPICAEYTSEEFAINVTDKSAIEWIRNTDEGRAWADSLGFEEPFFFTPERECTAEDE
                     RPLLFFSNLTEGQTITESPLDVWVVVSAVKDFKEFRLDYGLGSDPADWETLVEGLTAP
                     LNQPGLIGTWDISSLPAGPITLRLHLDSDRDTYAEKIIHINVQVPTLTPTPTPTPTQT
                     PTPTPTPTTTPTKPPDEP"
BASE COUNT          303 a          523 c          425 g          279 t
ORIGIN      
        1 atggccgctt ccacggcccg gtttcggtgc gctcggcgct ggccaattca tttaatatcc
       61 ccgcggtcaa ggcgctggaa tacattggca tttacgataa ccccgccacc gagtggcctg
      121 atgggttggt gccgtttgcc cagcggctgg gcatcaccac gctcacccgt aatgattacg
      181 gcctggcgct gacgctgggc ggcggtgatg tgagcctgct ggagatgact ggcgcctacg
      241 ccgtctttgc caacggcggg cagcaggtgc cgccggtagc catcacccgg attgtggatc
      301 acgaaggcaa cgtggtctat gaatatgaac cccccgagcc tgtgcaggcg ctgcgctccg
      361 aacacgccta cctgatcagt tcgatcctct cggacaatgc cgcccgcacg ccgatgttcg
      421 gcgccaattc ggtgctcaat ctgccgtttg ccgcggccgc caagaccggc accaccaacg
      481 actaccgcga taactggacc attggctaca cccccgacct ggtgacgggc gtgtgggtgg
      541 gcaatgccga ttacaccccc atgcagaaca ccaccggcct caccggtgcg gcgcccatct
      601 gggcgcagtt tatgcagttt gccgtgccgc agctcaccgg cggctacccc acccccttca
      661 cccgcccgcc cggcgtggtg gatctggtgg tctgctcggt ctccggggcg cagccttcgc
      721 agtggtgccc cgagcagcgc accgagatct ttgctgccga ccagccgccc gcccccgccg
      781 accaggacct gtggcaaaaa gtgctggtgg atacctggac ggggctgcgc gcctcgccga
      841 tttgcgccga gtacacctcg gaggaatttg ccatcaacgt gaccgataaa tcggccattg
      901 agtggattcg caacaccgac gaaggccgcg cctgggctga ttcactgggc tttgaagaac
      961 ccttcttctt tacccccgag cgtgaatgta ctgctgaaga cgagcgcccg ctgctgttct
     1021 tctccaacct caccgagggt cagacgatca ccgaaagccc gctggatgtc tgggtggtgg
     1081 tcagcgcggt caaggatttc aaagaattcc ggctggacta tggcctgggc agcgaccccg
     1141 ccgactggga aaccctggtg gaaggcctga ccgcgcccct gaaccagccc ggtttgatcg
     1201 gcacctggga tatcagcagc ctgcccgccg ggccaatcac cctgcggctg cacctggaca
     1261 gcgatcggga cacctacgcc gaaaaaatca ttcacatcaa cgtgcaggtg cctacgctca
     1321 cccccacacc tacccccacc cccacgcaaa cgcccacccc tacccccacg ccaaccacca
     1381 cccccaccaa accgccggac gaaccgtagg atttggaatt ttagatttga aatttgagat
     1441 ttgagattga ggataaagca gcggttgagc cagtatttac ccggcttaac cgctgcccta
     1501 aaacctgaat cccaaaacct aaaacccaga
//
LOCUS       DAOPVS010000314         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1386
            NODE_93927_length_1520_cov_63.286007, whole genome shotgun
            sequence.
ACCESSION   DAOPVS010000314 DAOPVS010000000
VERSION     DAOPVS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854591
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:45:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,400
            CDSs (total)                      :: 2,369
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93927_length_1520_cov_63.286007"
                     /isolate="AMR_MDS_1386"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKL11_11995"
     CDS             complement(<1..689)
                     /locus_tag="PKL11_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ14366.1"
                     /translation="MLTIFTAPKPFTNPHIDLIQRNAIRSWVALGPEVEVLLIGEEEG
                     LAEAARELGVRHLPQVQRNDQGTPLIPSIFNLARQETDKPYLAYVNADVILLPDVLEA
                     TRAAAAQARRFLMVGQRWDLDVRAPLDYPAGWPERLHAEVAQRGRLHPPAGSDYFIFP
                     RECFTDIPDLTVGRAGWDNWMIYAARRNGWRVIDGTDDVMIIHQDHDYSHLPGGQPHY
                     RQPETFENIRLA"
     gene            complement(692..>1520)
                     /locus_tag="PKL11_12000"
     CDS             complement(692..>1520)
                     /locus_tag="PKL11_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNZ14367.1"
                     /translation="LISPQHAKVEYNQPMTYPRTIAIIQARMSSSRLPGKVLLDLGGQ
                     PALAWVIQRARRARLLSGVLTATTTDPADDPIAELCARLDTPCFRGHPFDVLDRYYQA
                     ARSAEAEAIIRITADCPLIDPAQIDELIAAFFEQGVDFACNRLPPPWHRTYPIGLDLE
                     MCSFAGLERAWREATLKHEREHVLPYFYDEPGRFKVLQLNAEADYGHLRWTLDTPADL
                     EVLRVIFEHFRTQPDFSWRDVLAFWQANPQLAERNAAIQHKTMTDVDDRYQPDPGEK"
BASE COUNT          292 a          457 c          488 g          283 t
ORIGIN      
        1 gccagccgga tgttctcaaa cgtctcgggc tgacggtagt ggggctgccc gccgggcagg
       61 tggctgtaat cgtgatcctg gtgaataatc attacatcat cggtgccgtc aatcacccgc
      121 cagccgttgc gccgggcggc gtaaatcatc cagttatccc agccggcgcg gcccaccgtc
      181 aggtctggaa tatccgtaaa gcactcgcgc gggaagatga aataatcgct gcctgccggg
      241 gggtgcagcc ggccgcgctg ggctacctcg gcgtgcaggc gctctggcca gcctgccgga
      301 taatccaacg gggcgcgcac gtccaggtcc cagcgctgcc ccaccatcaa aaagcgccgg
      361 gcttgcgccg ccgcggcgcg ggtggcttcc agcacatcgg gcagcagaat cacatcagca
      421 ttgacgtagg ccaggtaggg cttgtcggtc tcctgccggg ccaggttgaa aatcgaggga
      481 atcagcgggg tgccctggtc gttgcgctgc acctgcggca aatgccgcac gcccagctcg
      541 cgggcagctt ctgccagccc ttcttcctcg ccaatcagca gcacttccac ttcaggcccc
      601 agcgccaccc atgagcggat ggcgttgcgc tgaatcaggt caatgtgtgg gttggtgaag
      661 ggcttgggcg cggtaaaaat cgtcagcatg gctatttctc cccggggtca ggctgatagc
      721 ggtcatccac atcggtcatg gttttgtgtt gaatcgccgc gttgcgttcg gcaagctgcg
      781 gattggcctg ccagaaggcc agtacatccc gccagctaaa atctggctgt gttctgaagt
      841 gctcaaagat aacgcgcaat acctccagat cggccggggt atccagcgtc cagcgcaggt
      901 gcccataatc ggcttcagca ttgagctgca gcaccttgaa gcgccccggc tcgtcataaa
      961 aatagggcag cacatgctcg cgctcatgct tcagcgtggc ttcacgccag gcgcgttcca
     1021 gcccggcaaa gctgcacatt tccaggtcca ggccaatggg gtaggtgcgg tgccagggcg
     1081 gcggcaggcg gttgcaggca aaatcgacgc cctgctcaaa gaatgcggca atcaactcat
     1141 caatctgggc cggatctatc aacgggcagt ctgcggtgat gcggatgatg gcctcggctt
     1201 cagccgagcg ggccgcctgg tagtagcgat ccagcacgtc aaaggggtgc ccgcggaaac
     1261 agggcgtatc caaccgggca cataattcgg caatagggtc atccgccggg tcggtggtgg
     1321 tggctgtcag cacaccgctc agcaggcggg cgcgccgggc gcgttgaatc acccaggcca
     1381 gcgccggctg accgcccagg tcgagcagca ctttgcccgg cagccgggag gagctcatac
     1441 gagcttgaat gatggcaatg gtgcgaggat aggtcatggg ctgattgtac tcaacttttg
     1501 cgtgctgtgg cgaaatcaat
//
